Southwest Area Fleet
Upon commissioning, "Ro-110" was attached to the Sasebo Naval District. She was reassigned to Submarine Squadron 11 on 10 July 1943. On 10 November 1943 she was reassigned to Submarine Division 30 in Submarine Squadron 8 in the Southwest Area Fleet, and she departed Sasebo, Japan, that day and moved to Tachibana Bay on the coast of Kyushu. On 12 November 1944 she got underway from Tachibana Bay bound for Penang in Japanese-occupied British Malaya, which she reached on 24 November 1943.