Margaret of Valois
Nicot served as the French ambassador in Lisbon, Portugal from 1547 to 1559 under Henry II, under king Francis II from 1559 to 1560, and from 1560 to 1574 under Charles IX. As a 29-year-old in 1559, he was sent from France to Portugal to negotiate the marriage of six-year-old princess Margaret of Valois to five-year-old King Sebastian of Portugal.