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Pinole Creek supports a mostly native fish assemblage including rainbow/steelhead trout, California roach ("Lavinia symmetricus"), Sacramento sucker ("Catostomus occidentalis"), Threespine stickleback ("Gasterosteus aculeatus"), Prickly sculpin ("Cottus asper"). Mosquitofish ("Gambusia affinis") and Common carp ("Cyprinus carpio") are nonnative fishes found predominately in the lower section of Pinole Creek, below Interstate 80.