土佐派
Masanobu trained his sons Kanō Motonobu (1476–1559) and the younger Yukinobu (or Utanosuke). Motonobu is usually credited with establishing the school's distinctive technique and style, or rather different styles, which brought a firmer line and stronger outlines to paintings using Chinese conventions. Less interest was taken in subtle effects of atmospheric recession that in the Chinese models, and elements in the composition tend to be placed at the front of the picture space, often achieving decorative effects in a distinctively Japanese way. Motonobu married the daughter of Tosa Mitsunobu, the head of the Tosa school, which continued the classic Japanese yamato-e style of largely narrative and religious subjects, and Kanō paintings subsequently also included more traditional Japanese subjects typical of that school.