지쿠젠 국
France was the last European country to embrace variolation. It was not until an outbreak of smallpox in Paris in 1752 nearly killed the heir to the French throne that the public embraced the practice after seeing the prince variolated. Similarly in Japan, Chinese merchant Li Jen-Shan proposed the method of traditional Chinese intranasal variolation after a severe smallpox outbreak in Nagasaki in 1744. This led Japanese physician to variolate children using a human smallpox vaccination method during an outbreak in Chikuzen Province from 1789 to 1790. There were no deaths among the children, and they all appeared to be protected.