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Agersø is located 3 km off the coast of Zealand, with ferry departures to Agersø By leaving from Stignæs Harbour. It is located between the Great Belt and Smålandsfarvandet. The island has a total area of 684, which includes former island of Egholm north of Agersø, which has now grown together with Agersø. Most of Agersø is cultivated, though there are sizable beach meadows on the southern parts of the island. Several amphibians rare in Denmark are found on Agersø. These include European fire-bellied toad, natterjack toad and European green toad. Breeding birds on the island include great black-backed gull, common gull, European herring gull, common eider, mallard, northern shoveler, northern pintail and gadwall.