Hijikata Toshizō
Serizawa's erratic and disruptive behavior in Kyoto eventually led to Matsudaira Katamori of Aizu giving the Shinsengumi an order to assassinate Serizawa and his group. On October 19, 1863, Niimi Nishiki, a member of the Serizawa faction was forced by Yamanami Keisuke and Hijikata Toshizō to commit seppuku for breaking regulations. On October 30 (or October 28), a few selected Shinsengumi members led by Hijikata went into the Yagi Gennojō's house and assassinated Serizawa, his woman Oume, and Hirayama Goro, with Hirama Jūsuke been the only survivor fled that night. All this infighting left Kondō as leader. Three months later, Noguchi Kenji was ordered to commit seppuku for an unknown reason.