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A church at the site initially was built in the 4th century over the house of consul Lucius Fabius Cilo. Possibly the ancient "Titulus Tigridae", the basilica was consecrated to St Balbina (died c. 130) in circa the year 600 by Pope Gregory I. It underwent many revisions since then, including by Pope Gregory III in 751, Pope Paul II in 1464, and by Cardinal Pompeo Arrigoni in 1600. Initially affiliated with Augustinians, the allegiance changed to the secular priests of Naples by Pope Innocent XII.