Reverse transcriptase
The genome of the virus has been cloned and cDNA probes are available for diagnosis. Reverse transcriptase polymerase chain reaction (RT-PCR) methods have been developed for detection of TSV and are very sensitive. Real-time techniques allow for quantification of the virus. The IQ2000TM TSV detection system, a RT-PCR method, is said to have a detection limit of 10 copies per reaction.