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"Twinkle Twinkle" was released as a CD single on June 13, 2012 through Sony Music Associated Records. It also includes the B-side track, "First Kiss". An anime version of "Twinkle Twinkle" was used as the ending theme song of the Naruto spin-off, "Naruto SD: Rock Lee and his Ninja Pals" aired on TV Tokyo. Sony Music Japan released the official music video of "Twinkle Twinkle" on May 11.