Potential energy
ATP producing transporters run in the opposite direction of ATP Utilizing transporters. These proteins transport ions from high to low concentration with the gradient but in the process ATP is formed. Potential energy in the form of the concentration gradient is used to generate ATP. In animals, this ATP synthesis takes place in the mitochondria using F- type ATPase otherwise known as ATP synthase. This process utilizes the electron transport chain in a process called oxidative phosphorylation. V-type ATPase serves the opposite function as F-type ATPase and is used in plants to hydrolyze ATP to create a proton gradient. Examples of this are lysosomes that use V-type ATPase acidify vesicles or plant vacuoles during process of photosynthesis in the chloroplasts. This process can be regulated through various methods such as pH.