Russell's paradox
In the presence of the axiom schema of separation, Russell's paradox becomes a proof that there is no set of all sets. The axiom of regularity together with the axiom of pairing also prohibit such a universal set. However, Russell's paradox yields a proof that there is no "set of all sets" using the axiom schema of separation alone, without any additional axioms. In particular, ZF without the axiom of regularity already prohibits such a universal set.