Château d'Amboise
Her childhood was spent in the French royal nursery of the Château de Saint-Germain-en-Laye with her sisters Elisabeth and Claude, under the care of Charlotte de Vienne, baronne de Courton, "a wise and virtuous lady greatly attached to the Catholic religion". After her sisters' weddings, Margaret grew up in the Château d'Amboise with her brothers Henry and Francis. During her childhood, her brother Charles IX gave her the nickname of "Margot".