Alkaline phosphatase
In normal stem cells, the activity of alkaline phosphatase activity is lowered upon differentiation. Trichostatin A causes the cells to maintain the activity of alkaline phosphatase. Trichostatin A can cause reversible inhibition of mammalian histone deacetylase, which leads to the inhibition of cell growth Significant increase in alkaline phosphatase extinction was observed when Trichostatin A was withdrawn after three days. Alkaline phosphatase activity correlates with the morphology changes. Initial deacetylation of histone is required for embryonic stem cell differentiation.