DNA polymerase
The enzyme linked antibodies inactivate the Taq DNA polymerase. The antibodies link and bind to the polymerase, preventing early DNA amplification which could occur at lower temperatures. Once the optimal annealing temperature is met, the antibodies will begin to degrade and dissociate, releasing the Taq DNA polymerase into the reaction and allowing the amplification process to start. Platinum Taq DNA polymerase and AccuStart Taq DNA polymerase ( both developed by Ayoub Rashtchian at Life technologies and Quanta BioSciences, respectively) are examples of commercially available antibody based hot start Taq DNA polymerases. These Taq DNA polymerase are precomplexed with a mixture of monoclonal antibodies specific to Taq DNA polymerase.