石狩川
Attempts to stockpile botulinum toxin proved unsuccessful. Seiichi Endo – one of the members tasked with acquiring botulinum toxin – collected soil samples from the Ishikari River, and attempted to produce the toxin using three capacity fermenters. In total, around 50 batches of of a crude broth were producedhowever, the cult did not attempt to purify the broth (which mostly would have consisted of bacterial cultivation media; one member even fell into one of the fermenters and nearly drowned, but otherwise suffered no ill effects).