Polymerase chain reaction
Polymerase chain reaction (PCR) combined with restriction enzyme digestion of an amplified ribosomal DNA fragment, are now used to characterize "T. yallundae" isolates. Novartis produces a polymerase chain reaction (PCR) diagnostic tool that provides cereal growers with an efficient means for checking the progression of eyespot disease in cereals. The tool reveals the presence and extent of disease before symptoms develop and can identify the different eyespot strains including the W-type ("Tapesia yallundae"') and R-type ("Tapesia acuformis").