julilili42/papers huggingface dataset:
A Composite Osteomusculocutaneous Free Flap from the Medial Femoral Condyle for Reconstruction of Complex Defects
2011-03-18

This combined anatomic and clinical study illustrates the first experiences of an osteomyocutaneous flap from the medial femoral condyle for reconstruction of composite tissue defects. We analyzed the anatomic consistency and the vascular distribution of this flap and showed that muscle tissue can easily be added as a composite flap. Twenty-one flaps were harvested from fresh adult cadavers with careful identification of the origin and the course of the three different branches of the descending genicular artery. The corresponding skin areas and muscle portion were identified. The clinical application of this flap was described for closure of complex calcaneal defects. The cadaveric study presented a constant pedicle length and diameter of the arteries, combined with a constant venous drainage. Furthermore, the medial condyle provided a corticocancellous segment and separate vascularity for skin and muscle portions. In the case reports, satisfying results of bone union and soft tissue contouring were achieved. The medial femur condyle region is a reliable donor site for composite flaps, providing a good corticocancellous bony structure and a separate skin paddle, as well as a muscle portion. Its vascular distribution shows anatomic consistency. Despite long-term atrophy of muscle transplants, we believe the additional muscle tissue improves the reconstruction results and provides better soft tissue contouring.
Simultaneous multi-wavelength campaign on PKS 2005-489 in a high state
2011-03-18

The high-frequency peaked BL Lac object PKS 2005-489 was the target of a multi-wavelength campaign with simultaneous observations in the TeV γ-ray (H.E.S.S.), GeV γ-ray (Fermi/LAT), X-ray (RXTE, Swift), UV (Swift) and optical (ATOM, Swift) bands. This campaign was carried out during a high flux state in the synchrotron regime. The flux in the optical and X-ray bands reached the level of the historical maxima. The hard GeV spectrum observed with Fermi/LAT connects well to the very high energy (VHE, E &gt; 100 GeV) spectrum measured with H.E.S.S.
Depression comorbidity in spinocerebellar ataxia
2011-03-21

Abstract This is a description of the prevalence and profile of depressive symptoms in dominant spinocerebellar ataxia (SCA). Depressive symptoms were assessed in a convenience sample of 526 genetically confirmed and clinically affected patients (117 SCA1, 163 SCA2, 139 SCA3, and 107 SCA6) using the Patient Health Questionnaire (PHQ). In addition, depressive status according to the examiner and the use of antidepressants was recorded. Depression self‐assessment was compared with an interview‐based psychiatric assessment in a subset of 26 patients. Depression prevalence estimates were 17.1% according to the PHQ algorithm and 15.4% when assessed clinically. The sensitivity of clinical impression compared with PHQ classification was low (0.35), whereas diagnostic accuracy of PHQ compared with psychiatric interview in the subset was high. Antidepressants were used by 17.7% of patients and in &gt;10% of patients without current clinically relevant depressive symptoms. Depression profile in SCA did not differ from a sample of patients with major depressive disorder except for the movement‐related item. Neither depression prevalence nor use of antidepressants differed between genetic subtypes, with only sleep disturbance more common in SCA3. In a multivariate analysis, ataxia severity and female sex independently predicted depressive status in SCA. The PHQ algorithmic classification is appropriate for use in SCA but should stimulate further psychiatric evaluation if depression is indicated. Despite a higher risk for depression with more severe disease, the relation of depressive symptoms to SCA neurodegeneration remains to be shown. © 2011 Movement Disorder Society
Retinal nerve fiber layer loss in multiple system atrophy
2011-03-21

Additional Supporting Information may be found in the online version of this article. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
True language-level SQL debugging
2011-03-21

We demonstrate Habitat, a declarative observational debugger for SQL. Habitat facilitates true language-level (not: plan-level) debugging of, probably flawed, SQL queries that yield unexpected results. Users may mark arbitrary SQL subexpressions---ranging from literals, over fragments of predicates, to entire subquery blocks---to observe whether these evaluate as expected.
When movement patterns turn into events: Implications for the recognition of spatial configurations from different viewpoints
2011-03-21

In the present paper, we examine the spatial representation of dynamic scenes. Particularly, we focus on the question whether recognition performance is viewpoint dependent or viewpoint invariant. In two experiments, the processing of events with regard to their spatial dimension was investigated. Dynamic scenes were varied in a manner that either retained or suppressed their event characteristics. It could be shown that the meaningful variant of the scene led to a viewpoint independent recognition performance, whereas the meaningless variant led to a viewpoint dependent recognition performance. Taken together, the experiments demonstrate that the event character of dynamic scenes enables viewers to encode the scene in a highly economic way and, therefore, has profound implications for the quality of spatial representation in terms of its abstract, viewpoint independent character.
Conserved and Unique Features of the Maize (Zea mays L.) Root Hair Proteome
2011-03-22

Root hairs are unicellular extensions of specialized epidermis cells. Under limiting conditions, they significantly increase the water and nutrient uptake capacity of plants by enlarging their root surface. Thus far, little is known about the initiation and growth of root hairs in the monocot model species maize. To gain a first insight into the protein composition of these specialized cells, the 2573 most abundant proteins of maize root hairs attached to four-day-old primary roots of the inbred line B73 were identified by combining 1DE with nanoLC-MS/MS in a shotgun proteomic experiment. Among the identified proteins, homologues of 252 proteins have been previously associated with root hair formation and development in other species. Comparison of the root hair reference proteome of the monocot species maize with the previously published root hair proteome of the dicot species soybean revealed conserved, but also unique, protein functions in root hairs of these two major groups of flowering plants.
Interaction of the general transcription factor TnrA with the PII‐like protein GlnK and glutamine synthetase in Bacillus subtilis
2011-03-22

TnrA is a master transcription factor regulating nitrogen metabolism in Bacillus subtilis under conditions of nitrogen limitation. When the preferred nitrogen source is in excess, feedback‐inhibited glutamine synthetase (GS) has been shown to bind TnrA and disable its activity. In cells grown with an energetically unfavorable nitrogen source such as nitrate, TnrA is fully membrane‐bound via a complex of AmtB and GlnK, which are the transmembrane ammonium transporter and its cognate regulator, respectively, originally termed NrgA and NrgB. The complete removal of nitrate from the medium leads to rapid degradation of TnrA in wild‐type cells. In contrast, in AmtB‐deficient or GlnK‐deficient strains, TnrA is neither membrane‐bound nor degraded in response to nitrate depletion. Here, we show that TnrA forms either a stable soluble complex with GlnK in the absence of AmtB, or constitutively binds to GS in the absence of GlnK. In vitro , the TnrA C‐terminus is responsible for interactions with either GS or GlnK, and this region appears also to mediate proteolysis, suggesting that binding of GlnK or GS protects TnrA from degradation. Surface plasmon resonance detection assays have demonstrated that GS binds to TnrA not only in its feedback‐inhibited form, but also in its non‐feedback‐inhibited form, although less efficiently. TnrA binding to GlnK or GS responds differentially to adenylate nucleotide levels, with ATP weakening interactions with both partners. Structured digital abstract tnrA binds to glnK by surface plasmon resonance ( View interaction ) GS binds to tnrA by pull down ( View interaction ) tnrA binds to glnK by pull down ( View interaction ) tnrA binds to GS by pull down ( View interaction ) GS physically interacts with tnrA by anti bait coimmunoprecipitation ( View interaction ) glnK binds to tnrA by pull down ( View interaction ) glnK physically interacts with tnrA by anti bait coimmunoprecipitation ( View interaction ) tnrA physically interacts with GS by anti bait coimmunoprecipitation ( View interaction ) tnrA physically interacts with glnK by anti bait coimmunoprecipitation ( View interaction ) tnrA binds to tnrA by cross‐linking study ( View interaction ) <j
Genetic patterns in the Lathyrus pannonicus complex (Fabaceae) reflect ecological differentiation rather than biogeography and traditional subspecific division
2011-03-22

The endangered European relict species complex Lathyrus pannonicus shows distinct morphological variation, reflected by the number of subspecies recognized, and complicated patterns of genetic variation. The traditionally recognized subspecies appear to possess different ecological preferences and disjunct distributions, particularly in the western range of the species. In this study, L. pannonicus was investigated by the correlation of distance matrices based on phytosociological, ecological, molecular and morphological data. Ecological characteristics of selected stands of L. pannonicus throughout Europe were assessed using 'Ellenberg values' of all the constituent taxa in phytosociological relevés. Genetic distances were calculated using recently developed methods to analyse high degrees of intra-individual nuclear-encoded internal transcribed spacer variability. We found that the remarkable genetic (and morphological) diversity in L. pannonicus could not be explained solely by the fragmentation of the distributional range. Instead, patterns of morphological and genetic differentiation were a reflection of the moisture regime in the sampled stands. Two major lineages could be identified: (1) a lineage adapted to dry conditions (Ellenberg indicator F-value ≤ 3.5) and (2) a lineage preferring moist conditions (F-value ≥ 4.5). Although both lineages occurred in close proximity in the Pannonian area, they appeared to be reproductively isolated in general. Further data are needed to determine whether these genetically and ecologically defined lineages, or ecospecies, within the L. pannonicus species complex can be formalized as (Linnaean) species or subspecies.
Gravitational wave asteroseismology with fast rotating neutron stars
2011-03-23

We investigate damping and growth times of the f-mode for rapidly rotating stars and a variety of different polytropic equations of state in the Cowling approximation. We discuss the differences in the eigenfunctions of co- and counterrotating modes and compute the damping times of the f-mode for several EoS and all rotation rates up to the Kepler-limit. This is the first study of the damping/growth time of this type of oscillations for fast rotating neutron stars in a general relativistic framework. We use these frequencies and damping/growth times to create robust empirical formulae which can be used for gravitational wave asteroseismology. The estimation of the damping/growth time is based on the quadrupole formula and our results agree very well with Newtonian ones in the appropriate limit.
Cellular FLICE-inhibitory Protein (cFLIP) Isoforms Block CD95- and TRAIL Death Receptor-induced Gene Induction Irrespective of Processing of Caspase-8 or cFLIP in the Death-inducing Signaling Complex
2011-03-23

Death receptors (DRs) induce apoptosis but also stimulate proinflammatory "non-apoptotic" signaling (e.g. NF-κB and mitogen-activated protein kinase (MAPK) activation) and inhibit distinct steps of DR-activated maturation of procaspase-8. To examine whether isoforms of cellular FLIP (cFLIP) or its cleavage products differentially regulate DR signaling, we established HaCaT cells expressing cFLIP(S), cFLIP(L), or mutants of cFLIP(L) (cFLIP(D376N) and cFLIP(p43)). cFLIP variants blocked TRAIL- and CD95L-induced apoptosis, but the cleavage pattern of caspase-8 in the death inducing signaling complex was different: cFLIP(L) induced processing of caspase-8 to the p43/41 fragments irrespective of cFLIP cleavage. cFLIP(S) or cFLIP(p43) blocked procaspase-8 cleavage. Analyzing non-apoptotic signaling pathways, we found that TRAIL and CD95L activate JNK and p38 within 15 min. cFLIP variants and different caspase inhibitors blocked late death ligand-induced JNK or p38 MAPK activation suggesting that these responses are secondary to cell death. cFLIP isoforms/mutants also blocked death ligand-mediated gene induction of CXCL-8 (IL-8). Knockdown of caspase-8 fully suppressed apoptotic and non-apoptotic signaling. Knockdown of cFLIP isoforms in primary human keratinocytes enhanced CD95L- and TRAIL-induced NF-κB activation, and JNK and p38 activation, underscoring the regulatory role of cFLIP for these DR-mediated signals. Whereas the presence of caspase-8 is critical for apoptotic and non-apoptotic signaling, cFLIP isoforms are potent inhibitors of TRAIL- and CD95L-induced apoptosis, NF-κB activation, and the late JNK and p38 MAPK activation. cFLIP-mediated inhibition of CD95 and TRAIL DR could be of crucial importance during keratinocyte skin carcinogenesis and for the activation of innate and/or adaptive immune responses triggered by DR activation in the skin.
Irritable bowel syndrome symptoms among German students
2011-03-23

IBS is a common syndrome among German university students and goes along with impaired health-related QoL. According to our data, students reporting recurrent abdominal pain in childhood are especially at risk for IBS.
Sensorimotor rhythm-based brain–computer interface training: the impact on motor cortical responsiveness
2011-03-24

The main purpose of electroencephalography (EEG)-based brain-computer interface (BCI) technology is to provide an alternative channel to support communication and control when motor pathways are interrupted. Despite the considerable amount of research focused on the improvement of EEG signal detection and translation into output commands, little is known about how learning to operate a BCI device may affect brain plasticity. This study investigated if and how sensorimotor rhythm-based BCI training would induce persistent functional changes in motor cortex, as assessed with transcranial magnetic stimulation (TMS) and high-density EEG. Motor imagery (MI)-based BCI training in naïve participants led to a significant increase in motor cortical excitability, as revealed by post-training TMS mapping of the hand muscle's cortical representation; peak amplitude and volume of the motor evoked potentials recorded from the opponens pollicis muscle were significantly higher only in those subjects who develop a MI strategy based on imagination of hand grasping to successfully control a computer cursor. Furthermore, analysis of the functional brain networks constructed using a connectivity matrix between scalp electrodes revealed a significant decrease in the global efficiency index for the higher-beta frequency range (22-29 Hz), indicating that the brain network changes its topology with practice of hand grasping MI. Our findings build the neurophysiological basis for the use of non-invasive BCI technology for monitoring and guidance of motor imagery-dependent brain plasticity and thus may render BCI a viable tool for post-stroke rehabilitation.
Workload measurement in a communication application operated through a P300-based brain–computer interface
2011-03-24

Advancing the brain-computer interface (BCI) towards practical applications in technology-based assistive solutions for people with disabilities requires coping with problems of accessibility and usability to increase user acceptance and satisfaction. The main objective of this study was to introduce a usability-oriented approach in the assessment of BCI technology development by focusing on evaluation of the user's subjective workload and satisfaction. The secondary aim was to compare two applications for a P300-based BCI. Eight healthy subjects were asked to use an assistive technology solution which integrates the P300-based BCI with commercially available software under two conditions--visual stimuli needed to evoke the P300 response were either overlaid onto the application's graphical user interface or presented on a separate screen. The two conditions were compared for effectiveness (level of performance), efficiency (subjective workload measured by means of NASA-TXL) and satisfaction of the user. Although no significant difference in usability could be detected between the two conditions, the methodology proved to be an effective tool to highlight weaknesses in the technical solution.
Insulin Sensitivity and Liver Fat: Role of Iron Load
2011-03-24

HIL explains part of the variation between LF and IS. The mechanism by which iron load induces insulin resistance is possibly independent of the pathways involved in insulin resistance induced by fatty liver disease.
Magnetic field-induced reactions on the surface of chloroaluminum phthalocyanine thin films
2011-03-24

The μ-(oxo)bis[phthalocyaninato] aluminum(III) (AlPc)2O films, with the crystallites oriented preferably in one direction, were obtained via chemical transformation of chloroaluminum(III) phthalocyanine AlClPc film upon its annealing in magnetic field. A comparative analysis of the influence of postdeposition annealing without and under applied magnetic field of 1 T on composition and morphology of AlClPc films has been carried out. The chemical transformation of AlClPc to (AlPc)2O on the substrate surface is studied by the methods of UV-vis and infrared spectroscopies, Raman, x-ray photoelectron spectroscopy as well as atomic force microscopy. Two interesting effects were observed upon heating the AlClPc films in magnetic field of 1 T. First, the temperature of the chemical transformation of AlClPc to (AlPc)2O decreased from 300 °C to 200 °C when magnetic field was applied during postdeposition annealing. Second, the formation of (AlPc)2O films with elongated crystallites with a preferential orientation was observed. The heating of (AlPc)2O films in a magnetic field at the same conditions did not demonstrate any effect on the structure and morphology of these films.
Determinants of Central Processing Order in Psychological Refractory Period Paradigms: Central Arrival Times, Detection Times, or Preparation?
2011-03-24

Three psychological refractory period (PRP) experiments were conducted to assess the effect of central arrival times at the bottleneck on task order scheduling. In Experiment 1, a visual first task (plus–minus symbol discrimination) was combined with an auditory second task (left–right tone judgement) in a standard PRP paradigm with constant task order. In Experiment 2, the order of the tasks varied unpredictably. In Experiment 3, visual–auditory dual-task trials were randomly mixed with single-task trials. To dissociate central arrival times from stimulus detection times, the perceptual stage of the visual task was extended using stimulus degradation. Most importantly, no evidence for a first-come, first-served principle at the central bottleneck was found with the employed paradigms. Instead, the results indicated that preparation (Experiment 1) and the detection times of the stimuli (Experiments 2 and 3) were the main determinants of central processing order in the present study. In the light of previous research, the results indicate that central processing order can be influenced by various factors. The interplay between these factors seems to depend highly on the conditions and requirements of the employed experimental paradigm.
Disrupting the EMMPRIN (CD147)–Cyclophilin A Interaction Reduces Infarct Size and Preserves Systolic Function After Myocardial Ischemia and Reperfusion
2011-03-25

Objective— Inflammation and proteolysis crucially contribute to myocardial ischemia and reperfusion injury. The extracellular matrix metalloproteinase inducer EMMPRIN (CD147) and its ligand cyclophilin A (CyPA) may be involved in both processes. The aim of the study was to characterize the role of the CD147 and CyPA interplay in myocardial ischemia/reperfusion (I/R) injury. Methods and Results— Immunohistochemistry showed enhanced expression of CD147 and CyPA in myocardial sections from human autopsies of patients who had died from acute myocardial infarction and from mice at 24 hours after I/R. At 24 hours and 7 days after I/R, the infarct size was reduced in CD147 +/− mice vs CD147 +/+ mice (C57Bl/6), in mice (C57Bl/6) treated with monoclonal antibody anti-CD147 vs control monoclonal antibody, and in CyPA −/− mice vs CyPA +/+ mice (129S6/SvEv), all of which are associated with reduced monocyte and neutrophil recruitment at 24 hours and with a preserved systolic function at 7 days. The combination of CyPA −/− mice with anti-CD147 treatment did not yield further protection compared with either inhibition strategy alone. In vitro, treatment with CyPA induced monocyte chemotaxis in a CD147- and phosphatidylinositol 3-kinase–dependent manner and induced monocyte rolling and adhesion to endothelium (human umbilical vein endothelial cells) under flow in a CD147-dependent manner. Conclusion— CD147 and its ligand CyPA are inflammatory mediators after myocardial ischemia and reperfusion and represent potential targets to prevent myocardial I/R injury.
Implicit, Predictive Timing Draws upon the Same Scalar Representation of Time as Explicit Timing
2011-03-25

It is not yet known whether the scalar properties of explicit timing are also displayed by more implicit, predictive forms of timing. We investigated whether performance in both explicit and predictive timing tasks conformed to the two psychophysical properties of scalar timing: the Psychophysical law and Weber's law. Our explicit temporal generalization task required overt estimation of the duration of an empty interval bounded by visual markers, whereas our temporal expectancy task presented visual stimuli at temporally predictable intervals, which facilitated motor preparation thus speeding target detection. The Psychophysical Law and Weber's Law were modeled, respectively, by (1) the functional dependence between mean subjective time and real time (2) the linearity of the relationship between timing variability and duration. Results showed that performance for predictive, as well as explicit, timing conformed to both psychophysical properties of interval timing. Both tasks showed the same linear relationship between subjective and real time, demonstrating that the same representational mechanism is engaged whether it is transferred into an overt estimate of duration or used to optimise sensorimotor behavior. Moreover, variability increased with increasing duration during both tasks, consistent with a scalar representation of time in both predictive and explicit timing. However, timing variability was greater during predictive timing, at least for durations greater than 200 msec, and ascribable to temporal, rather than non-temporal, mechanisms engaged by the task. These results suggest that although the same internal representation of time was used in both tasks, its external manifestation varied as a function of temporal task goals.