julilili42/papers huggingface dataset:
Transplanting gravid lizards to high elevation alters maternal and embryonic oxygen physiology, but not reproductive success or hatchling phenotype
2019-01-01

Increased global temperatures have opened previously inhospitable habitats, such as at higher elevations. However, the reduction of oxygen partial pressure with increase in elevation represents an important physiological constraint that may limit colonization of such habitats, even if the thermal niche is appropriate. To test the mechanisms underlying the response to ecologically-relevant levels of hypoxia, we performed a translocation experiment with the common wall lizard (Podarcis muralis), a widespread European lizard amenable to establishing populations outside its natural range. We investigated the impacts of hypoxia on the oxygen physiology and reproductive output of gravid common wall lizards and the subsequent development and morphology of their offspring. Lowland females transplanted to high elevations increased their haematocrit and haemoglobin concentration within days and maintained routine metabolism compared to lizards kept at native elevations. However, transplanted lizards suffered from increased reactive oxygen metabolite production near the oviposition date, suggesting a cost of reproduction at high elevation. Transplanted females and females native to different elevations did not differ in reproductive output (clutch size, egg mass, relative clutch mass, or embryonic stage at oviposition) or in post-oviposition body condition. Developing embryos reduced heart rates and prolonged incubation times at high elevations within the native range and at extreme high elevations beyond the current range, but this reduced oxygen availability did not affect metabolic rate, hatching success, or hatchling size. These results suggest that this opportunistic colonizer is capable of successfully responding to novel environmental constraints in these important life-history stages.
Differences in novel food response betweenPongoandPan
2019-01-01

The diversity of great ape diets requires behavioral flexibility. Consequently, the exploration of potentially novel food sources is supposedly beneficial, but simultaneously, apes show high neophobia to prevent harmful and poisonous food intake. Social information, such as presence of group members or observations of non‐naïve, experienced individuals have been demonstrated to affect the acceptance of novel food items in primates. Sociality may have evolutionary effects on the response of apes to novel foods. Here we assess the social information hypothesis , which predicts that selection favors higher neophobia in species where social information is abundant. We report the results from 134 great apes housed in multiple facilities from four closely related species that naturally differ in their degree of sociality: Pongo pygmaeus , Pongo abelii , Pan troglodytes and Pan paniscus . We examined individuals’ reactions to novel foods when alone, which enabled us to detect any inherent differences and revealed significant distinctions between species. Chimpanzees and bonobos, that are naturally exposed to higher amounts of social information, were less likely to consume novel foods alone (showed higher neophobia) than the two more solitary orangutan species. Chimpanzees were especially cautious and showed higher explorative behaviors before tasting novel food than other species. Age influenced neophobia as younger individuals of all species took longer to taste novel foods than adults did.
The Oncolytic Herpes Simplex Virus Talimogene Laherparepvec Shows Promising Efficacy in Neuroendocrine Cancer Cell Lines
2019-01-01

Metastatic neuroendocrine cancer still constitutes a palliative situation, lacking promising treatment options. Oncolytic virotherapy, a novel type of virus-based immunotherapy, lyses tumor cells using genetically engineered viruses thereby activating the immune system to induce an optimized antitumor response which could bring down tumor masses to a stage of minimal residual tumor disease. The oncolytic vector talimogene laherparepvec (T-VEC, herpes simplex virus [HSV] type 1) has already shown excellent safety profiles in clinical studies and has become the first ever FDA/EMA-approved oncolytic virus (OV). This work presents a first preclinical assessment of this state-of-the-art OV, using a panel of human neuroendocrine tumor/neuroendocrine carcinoma (NET/NEC) cell lines. Cytotoxicity, transgene expression, and viral replication patterns were studied. Furthermore, the antiproliferative activity was compared to the one of mTOR inhibitor Everolimus and also interactions between the OV and Everolimus were evaluated. Moreover, virostatic effects of ganciclovir (GCV) on replication of T-VEC were assessed and electron microscopic pictures were taken to comprehend viral envelopment and details of the replication cycle of T-VEC in human neuroendocrine cancer. It could be shown that T-VEC infects, replicates in, and lyses human NET/NEC cells exhibiting high oncolytic efficiencies already at quite low virus concentrations. Interestingly, Everolimus was not found to have any relevant impact on rates of viral replication, but no additive effects could be proved using a combinatorial therapy regimen. On the other hand, GCV was shown to be able to limit replication of T-VEC, thus establishing an important safety feature for future treatments of NET/NEC patients. Taken together, T-VEC opens up a promising novel treatment option for NET/NEC patients, warranting its further preclinical and clinical development.
Sn20(SitBu3)10Cl2– the largest metalloid group 14 cluster shows a raspberry-like arrangement of smaller units
2019-01-01

The reaction of a metastable Sn(<sc>i</sc>)Cl solution with NaSi<sup>t</sup>Bu<sub>3</sub>leads to Sn<sub>20</sub>(Si<sup>t</sup>Bu<sub>3</sub>)<sub>10</sub>C<sub>l2</sub>, where a raspberry-like cluster of cluster arrangement is realized, giving further insight into the formation process of a metalloid tin cluster from molecular precursors.
Comparison of Material-mediated Bone Regeneration Capacities of Sintered and Non-sintered Xenogeneic Bone Substitutes via 2D and 3D Data
2019-01-01

Osteoconductivity index is a reliable measurement parameter for determining the healing capacities of BSM. The observed differences between both measurement methods could be assigned to the resolution capacity of μ-CT data that did not allow for a precise interface distinction between both BSM and bone tissue. Histomorphometrical data based on histological slides still allow for a more exact evaluation.
Imaging fibrosis in inflammatory diseases: targeting the exposed extracellular matrix
2019-01-01

In a variety of diseases, from benign to life-threatening ones, inflammation plays a major role. Monitoring the intensity and extent of a multifaceted inflammatory process has become a cornerstone in diagnostics and therapy monitoring. However, the current tools lack the ability to provide insight into one of its most crucial aspects, namely, the alteration of the extracellular matrix (ECM). Using a radiolabeled platelet glycoprotein VI-based ECM-targeting fusion protein (GPVI-Fc), we investigated how binding of GPVI-Fc on fibrous tissue could uncover the progression of several inflammatory disease models at different stages (rheumatoid arthritis, cutaneous delayed-type hypersensitivity, lung inflammation and experimental autoimmune encephalomyelitis). <b><i>Methods:</i></b> The fusion protein GPVI-Fc was covalently linked to 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) and subsequently labeled with <sup>64</sup>Cu. We analyzed noninvasively <i>in vivo</i><sup>64</sup>Cu-GPVI-Fc accumulation in murine cutaneous delayed-type hypersensitivity, anti-glucose-6-phosphate isomerase serum-induced rheumatoid arthritis, lipopolysaccharide-induced lung inflammation and an experimental autoimmune encephalomyelitis model. Static and dynamic Positron Emission Tomography (PET) of the radiotracer distribution was performed <i>in vivo</i>, with <i>ex vivo</i> autoradiography confirmation, yielding quantitative accumulation and a distribution map of <sup>64</sup>Cu-GPVI-Fc. <i>Ex vivo</i> tissue histological staining was performed on harvested samples to highlight the fusion protein binding to collagen I, II and III, fibronectin and fibrinogen as well as the morphology of excised tissue. <b><i>Results:</i></b><sup>64</sup>Cu-GPVI-Fc showed a several-fold increased uptake in inflamed tissue compared to control tissue, particularly in the RA model, with a peak 24 h after radiotracer injection of up to half the injected dose. Blocking and isotype control experiments indicated a target-driven accumulation of the radiotracer in the case of chronic inflammation. Histological analysis confirmed a prolonged accumulation at the inflammation site, with a pronounced colocalization with the different components of the ECM (collagen III and fibronectin notably). Binding of the fusion protein appeared to be specific to the ECM but unspecific to particular components. <b><i>Conclusion:</i></b> Imaging of <sup>64</sup>Cu-GPVI-Fc accumulation in the ECM matrix appears to be a promising candidate for monitoring chronic inflammation. By binding to exposed fibrous tissue (collagen, fibronectin, etc.) after extravasation, a new insight is provided into the fibrotic events resulting from a prolonged inflammatory state.
Higher-Faster-Farther: Maximum Performance Tests in the Assessment of Neurogenic Speech Impairment
2019-01-01

&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Maximum performance tests are widely used in dysarthria assessment. From a theoretical perspective, the motor demands of such tasks differ from those of speaking. Therefore, their validity as measures of dysarthric impairment needs to be established empirically. &lt;b&gt;&lt;i&gt;Patients and Methods:&lt;/i&gt;&lt;/b&gt; Maximum phonation time (MPT) and maximum syllable repetition rate (MRR) were compared with sentence reading/repetition tasks. In study 1, 130 patients with neurologic movement disorders and 130 healthy control participants were examined. Presence/severity of dysarthria was measured using psychometrically standardized auditory scales. In study 2, 16 healthy volunteers participated in an experiment designed to examine the intraspeaker variability of MPT, MRR, and sentence repetition across eight trials. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; Study 1: MPT made no reasonable contribution to the diagnosis of dysarthria or of any specific dimension of perceived speech impairment. MRR correlated with overall speech impairment but turned out to be an insensitive and highly unspecific statistical marker, afflicted with aetiology-specific errors. Study 2: compared with sentence repetition, both MPT and MRR demonstrated highly increased within-subject inconsistencies. &lt;b&gt;&lt;i&gt;Conclusion:&lt;/i&gt;&lt;/b&gt; The validity of MPT and MRR tasks as measures of dysarthria is still unsettled.
Predicted Indirectly ReCognizable HLA Epitopes (PIRCHE) Are Associated with Poorer Outcome after Single Mismatch Unrelated Donor Stem Cell Transplantation: A Study of the Cooperative Transplant Study Group (KTS) of the German Group for Bone Marrow and Stem Cell Transplantation (DAG-KBT)
2019-01-01

There is no established standard for selection of mismatched unrelated donors. Indirect recognition of HLA mismatches can be predicted using the model of “Predicted Indirectly ReCognizable HLA Epitopes” (PIRCHE). We performed a multicenter retrospective study evaluating the impact PIRCHE on outcome after allogeneic stem cell transplantation (allo-HSCT) from single mismatched (HLA 9/10 matched) unrelated donors. The study cohort included 424 adult recipients of HLA 9/10 matched unrelated donor transplants (9/10 MUD), treated for AML or MDS at 6 transplant centers across Germany. Detection of PIRCHE was associated with lower overall survival (OS) (47 vs. 57%, &lt;i&gt;p&lt;/i&gt; = 0.04), higher non-relapse mortality (NRM) (32 vs. 20%, &lt;i&gt;p&lt;/i&gt; = 0.05), and higher incidence of chronic graft-versus-host disease (GVHD) (49 vs. 31%, &lt;i&gt;p&lt;/i&gt; = 0.04) at 2 years. Cumulative incidence of acute GVHD grade 2–4 at 6 months was not significantly different (30 vs. 23%, &lt;i&gt;p&lt;/i&gt; = 0.2). OS for 9/10 MUD with no PIRCHE was similar to 10/10 MUD (57 vs. 55%). In multivariate analysis, PIRCHE retained negative impact on OS (RR 1.5, 95% CI 1.0–2.1, &lt;i&gt;p&lt;/i&gt; = 0.03) and NRM (RR 1.7, 95% CI 1.0–2.9, &lt;i&gt;p&lt;/i&gt; = 0.03). To the best of our knowledge, for the first time, we show the association of PIRCHE and survival outcome after allo-HSCT. The PIRCHE model, if validated in an independent cohort, may allow selection of permissible HLA mismatches that enable improved transplant outcome.
Strong second-harmonic generation from Au–Al heterodimers
2019-01-01

Strong second-harmonic generation from Au–Al heterodimers with a nanogap is observed and predicted, mainly attributed to coupling of plasmonic resonance in the fundamental field and synergistic driving from constituting nanoantennas in the SH field.
Comparison of two devices to simulate vision with intraocular lenses
2019-01-01

The assessment of the interdevice analysis for the Rassow telescope and the VirtIOL device revealed only a minor agreement between the two devices. In order to investigate vision with IOLs prior to a clinical validation, the use of the VirtIOL device is recommended.
Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats
2019-01-01

Neuropathic pain after spinal cord injury (SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy; nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells (fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium (sham) or fNSCs extracted from telencephalic vesicles (TV group) or the ventral medulla (VM group) of E/14 embryos. Sensory (von Frey filaments and hot plate) and motor (the Basso, Beattie, Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and VM groups at 4 and 5 weeks after transplantation, respectively. Moreover, mechanical allodynia improved in both the TV and VM groups at 8 weeks. No significant motor recovery was observed in the TV or VM groups compared with sham. Stereological analyses showed that ~70% of TV and VM cells differentiated into NeuN<sup>+</sup> neurons, with a high proportion of enkephalinergic and GABAergic cells in the TV group and enkephalinergic and serotoninergic cells in the VM group. Our study suggests that neuronal precursors from TV and VM, once implanted into the injured spinal cord, maturate into different neuronal subtypes, mainly GABAergic, serotoninergic, and enkephalinergic, and all subtypes alleviate pain, despite no significant motor recovery. The study was approved by the Animal Ethics Committee of the Medical School of the University of São Paulo (protocol number 033/14) on March 4, 2016.
Weak Associations of Morningness-Eveningness and Stability with Skin Temperature and Cortisol Levels
2019-01-01

Differences in daytime preferences can be described on the dimension of morningness-eveningness (continuous) or circadian typology (categorical) and are associated with our physiological functioning, which is reflected in body temperature and cortisol levels in the morning. The purpose of the present study was to explore the relationship between morningness-eveningness, stability and physiological markers (body temperature and cortisol) based on a three-dimensional conceptualization of morningness-eveningness using the Morningness-Eveningness-Stability Scale improved (MESSi). In contrast to previously used unidimensional measures, the MESSi determines circadian typology and its amplitude in three dimensions: Morning affect (MA), Eveningness (EV) and Stability/Distinctness (DI). Furthermore, the differences of the cortisol levels between weekday and weekend were examined. The sample (<i>N</i> = 42) consisted of extreme chronotypes (age 18-54 years; <i>M</i> = 24.8 years, <i>SD</i> = 5.83; 22 morning types [5 men and 17 women] and 20 evening types [8 men and 12 women]). The participants were asked to measure their skin temperature for one week and sample four saliva probes for cortisol determination. Morning types showed a better fit in the actual temperature data to the approximating data as compared to Evening types and showed a higher overall temperature. The Stability/Distinctness (DI) component of the MESSi was negatively correlated with the nadir. Morning types also showed higher cortisol levels than Evening types immediately after awakening. The cortisol levels were higher on a weekday compared to the weekend. To conclude, the present findings demonstrate that the skin temperature is weakly associated with morningness-eveningness and the stability of the circadian phase.
Compositional Generalization in Image Captioning
2019-01-01

Image captioning models are usually evaluated on their ability to describe a held-out set of images, not on their ability to generalize to unseen concepts. We study the problem of compositional generalization, which measures how well a model composes unseen combinations of concepts when describing images. State-of-the-art image captioning models show poor generalization performance on this task. We propose a multi-task model to address the poor performance, that combines caption generation and image--sentence ranking, and uses a decoding mechanism that re-ranks the captions according their similarity to the image. This model is substantially better at generalizing to unseen combinations of concepts compared to state-of-the-art captioning models.
Urban resilience in the face of fossil fuel dependency: the case of Rio de Janeiro’s urban mobility
2019-01-01

Abstract Long-term scenarios for mobility within cities usually neglect the energy supply challenge and how the implied risks affect urban mobility services. High levels of private transport and fossil fuel dependency tend to prevail in urban agglomerations of modern cities in many parts of the world. The resilience approach supports a new perspective on transportation solutions, not only based on how to consume less energy or emit less CO2, but how vulnerable urban mobility is in face of a fossil threat. This paper aims to assess the vulnerability of urban mobility in the face of fossil fuel threats under a social and geographical scope. We apply a case study with the city of Rio de Janeiro, confronting city inhabitants with a price increase of gasoline and oil-based public transportation. We found that more than 50% of the districts of Rio de Janeiro presents low-medium or low level of resilience of urban mobility. Furthermore, they are in areas with lower accessibility to metro stations and more citizens with reduced income levels.
Antitumour activity of Helix hemocyanin against bladder carcinoma permanent cell lines
2019-01-01

Hemocyanins are oxygen-transporting glycoproteins in molluscs and arthropods. In this study, we assayed the biomacromolecules from the molluscs Helix lucorum (HlH), Rapana venosa (RvH) and Megatura crenulata (KLH) including their functional units (FUs), for therapy of bladder cancer permanent cells. In vitro studies antitumour activities of these proteins were performed with bladder cancer permanent cell line CAL-29 and the normal urothelial cell line HL 10/29 in comparison to doxorubicin. The obtained results showed that the human tumour CAL-29 cell line is sensitive to the action of the tested hemocyanins and their isoforms. We observed a dose- and time-dependent inhibition of tumour cell growth after incubation with native HlH and two FUs (βc-HlH-a and FU βc-HlH-h); and of particular significance, FU βc-HlH-h showed a surprisingly stronger effect than that the doxorubicin-treated cells. Cells treated with βc-HlH-h, showed both, apoptotic and necrotic cells. In addition, two-dimensional polyacrylamide gel electrophoresis (PAGE) found for differential up-regulation of several proteins after hemocyanin treatment. Eight different down-regulated and two up-regulated proteins were identified, which may be associated with the apoptosis pathway. No inhibition of the normal urothelial cell line HL 10/29 was observed after treatment with HlH and its isoforms. The most effective inhibition of CAL-29 tumour cells was observed after treatment with βc-HlH-h, probably caused by a specific oligosaccharide structure of HlH with methylated hexoses. These results suggest that hemocyanin glycosylation plays an important role in its anticancer activity.
Microstructural analysis of Greenland ice using a cryogenic scanning electron microscope equipped with an electron backscatter diffraction detector
2019-01-01

Mass loss from ice sheets contributes to global sea level rise, and accelerated ice flow to the oceans is one of the major causes of rapid ice sheet mass loss. To understand flow dynamics of polar ice sheets, we need to understand deformation mechanisms of the polycrystalline ice in ice sheets. Laboratory experiments have shown that deformation of polycrystalline ice occurs largely by dislocation glide, which mainly depends on crystal orientation distribution. Grain size and impurities are also important factors that determine ice deformation mechanisms. Compared with ice formed during interglacial periods, ice formed during glacial periods, especially ice that forms cloudy bands, exhibits finer grain sizes and higher impurity concentrations. A previous report suggests the deformation rate of ice containing cloudy bands is higher than that of ice without cloudy bands. To examine the microstructures and deformation histories of ice in cloudy bands, we applied the electron backscatter diffraction (EBSD) technique to samples from the Greenland Ice Sheet using an environmental scanning electron microscope (ESEM) equipped with cold stages. Prior to the EBSD analysis, we optimised our ESEM/EBSD system and performed angular error assessment using artificial ice. In terms of c-and a-axis orientation distributions and grain orientation spread, we found little difference between samples taken from a cloudy band and those taken from an adjacent layer of clear ice. However, subgrain boundary density and orientation gradients were higher in the cloudy band, suggesting that there are more dislocations in the cloudy band than in the clear ice layer.