julilili42/papers huggingface dataset:
How Learners’ Visuospatial Ability and Different Ways of Changing the Perspective Influence Learning About Movements in Desktop and Immersive Virtual Reality Environments
2024-06-22

Abstract Virtual reality (VR) applications are developing rapidly, becoming more and more affordable, and offer various advantages for learning contexts. Dynamic visualizations are generally suitable for depicting continuous processes (e.g., different movement patterns), and particularly dynamic virtual 3D-objects can provide different perspectives on the movements. The present study investigated through a low immersive (desktop “VR”, Study 1) and a high immersive virtual environment (immersive VR; Study 2) the effectiveness of different interaction formats to view 3D-objects from different perspectives. Participants controlled either the orientation of the 3D-objects (Study 1, mouse interaction; Study 2, hand interaction via VR controllers) or their viewpoint in relation to the 3D-objects (Study 1, camera position; Study 2, position of participants’ own body). Additionally, the moderating influence of learners’ visuospatial ability was addressed. Dependent variables were pictorial recognition (easy, medium, difficult), factual knowledge, presence, and motion sickness. Results showed that higher-visuospatial-ability learners outperformed lower-visuospatial-ability learners. In Study 1, higher-visuospatial-ability learners showed higher recognition performance (difficult items) by controlling the camera position, whereas lower-visuospatial-ability learners suffered from this interaction format. In Study 2, higher-visuospatial-ability learners achieved better recognition performance (easy items) by controlling the 3D-models, whereas lower-visuospatial-ability learners tended to profit from moving around the 3D-objects (medium items). The immersive VR yielded more presence and higher motion sickness. This study clearly shows that different interaction formats to view 3D-objects from multiple perspectives in Desktop-VR are not transferable on a one-to-one basis into immersive VR. The results and implications for the design of virtual learning environments are discussed.
Long-term single molecule localization microscopy uncovers dynamic co-assembly of Lrp6 and Ror2 into Wnt-signalosomes
2024-06-22

Abstract The conserved Wnt signaling has been classified as two categories of canonical and noncanonical Wnt signaling. With a high promiscuity of Wnt signaling, how receptors from the two distinct pathways re-arrange in multi-protein signalosomes remains elusive. We here developed single-molecule tracking and localization microscopy based on labeling with reversibly binding nanobodies (rbTALM) for imaging receptor dynamics in the plasma membrane for extended time periods. To this end, we engineered nanobody-tag pairs with fine-tuned binding stabilities ensuring single-molecule tracking with high fidelity, yet continuous exchange of photobleached labels. Multicolor rbTALM imaging enabled simultaneous tracking and super-resolution imaging of three different Wnt co-receptors in the same cell for more than one hour at video rate. Time-lapse correlation analyses uncovered cooperative association of canonical and noncanonical Wnt co-receptors into a common, hybrid Wnt signalosome, demonstrating the exciting possibilities of rbTALM imaging for exploring nanoscale dynamics across millisecond to hour timescales.
Long-term perceptual priors drive confidence bias that favors prior-congruent evidence
2024-06-22

Abstract According to the Bayesian framework, both our perceptual decisions and confidence about those decisions are based on the precision-weighted integration of prior expectations and incoming sensory information. While it is generally assumed that priors influence both decisions and confidence in the same way, previous work has found priors to have a stronger impact at the confidence level, challenging this assumption. However, these patterns were found for high-level probabilistic expectations that are flexibly induced in the task context. It remains unclear whether this generalizes to low-level perceptual priors that are naturally formed through long term exposure. Here we investigated human participants’ confidence in decisions made under the influence of a long-term perceptual prior: the slow-motion prior. Participants viewed tilted moving-line stimuli for which the slow-motion prior biases the perceived motion direction. On each trial, they made two consecutive motion direction decisions followed by a confidence decision. We contrasted two conditions – one in which the prior impacted discrimination performance, and one in which it did not. We found a confidence bias favoring the condition in which the prior influenced discrimination decisions, even after accounting for performance differences. Computational modeling revealed this effect to be best explained by confidence using the prior-congruent evidence as an additional cue, beyond the posterior evidence used in the perceptual decision. This is in agreement with a confirmatory confidence bias favoring evidence congruent with low-level perceptual priors, revealing that, in line with high-level expectations, even long-term priors have a greater influence on the metacognitive level than on perceptual decisions. Author Summary Prior expectations play a critical role in shaping not only the perceptual inferences that we make, but also how confident we feel about those inferences. Bayesian confidence models capture that role, but assume priors to influence both decisions and confidence in the same way. Against this assumption, previous work has found dissociations in the influence of priors on decisions and confidence. However, that work has focussed only on high-level probabilistic priors, rather than the low-level perceptual priors that constrain our processing across many naturalistic situations. Here, we examine whether such dissociations arise under the influence of a low-level perceptual prior that naturally affects humans’ perception of motion, namely, the expectation that objects move slowly. We reveal evidence for such a dissociation: prior-congruent evidence impacts confidence to a greater extent than perceptual decisions. This suggests the existence of an implicit confidence bias favoring information that confirms prior beliefs, even in the case of long-term perceptual priors.
The impact of high‐IgE levels on metabolome and microbiome in experimental allergic enteritis
2024-06-23

Abstract Background The pathological mechanism of the gastrointestinal forms of food allergies is less understood in comparison to other clinical phenotypes, such as asthma and anaphylaxis Importantly, high‐IgE levels are a poor prognostic factor in gastrointestinal allergies. Methods This study investigated how high‐IgE levels influence the development of intestinal inflammation and the metabolome in allergic enteritis (AE), using IgE knock‐in (IgEki) mice expressing high levels of IgE. In addition, correlation of the altered metabolome with gut microbiome was analysed. Results Ovalbumin‐sensitized and egg‐white diet‐fed (OVA/EW) BALB/c WT mice developed moderate AE, whereas OVA/EW IgEki mice induced more aggravated intestinal inflammation with enhanced eosinophil accumulation. Untargeted metabolomics detected the increased levels of N‐tau‐methylhistamine and 2,3‐butanediol, and reduced levels of butyric acid in faeces and/or sera of OVA/EW IgEki mice, which was accompanied with reduced Clostridium and increased Lactobacillus at the genus level. Non‐sensitized and egg‐white diet‐fed (NC/EW) WT mice did not exhibit any signs of AE, whereas NC/EW IgEki mice developed marginal degrees of AE. Compared to NC/EW WT mice, enhanced levels of lysophospholipids, sphinganine and sphingosine were detected in serum and faecal samples of NC/EW IgEki mice. In addition, several associations of altered metabolome with gut microbiome—for example Akkermansia with lysophosphatidylserine—were detected. Conclusions Our results suggest that high‐IgE levels alter intestinal and systemic levels of endogenous and microbiota‐associated metabolites in experimental AE. This study contributes to deepening the knowledge of molecular mechanisms for the development of AE and provides clues to advance diagnostic and therapeutic strategies of allergic diseases.
Large-scale universality in quantum reaction-diffusion from Keldysh field theory
2024-06-24

We consider the quantum reaction-diffusion dynamics in d spatial dimensions of a Fermi gas subject to binary annihilation reactions A+A→∅. These systems display collective nonequilibrium long-time behavior, which is signalled by an algebraic decay of the particle density. Building on the Keldysh formalism, we devise a field theoretical approach for the reaction-limited regime, where annihilation reactions are scarce. Combining a perturbative expansion of the dissipative interaction with Euler-hydrodynamic scaling limit, we derive a description in terms of a large-scale universal kinetic equation. Our approach shows how the time-dependent generalized Gibbs ensemble assumption, which is often employed for treating low-dimensional nonequilibrium dissipative systems, emerges from systematic diagrammatics. It also allows us to exactly compute - for arbitrary spatial dimension - the decay exponent of the particle density. The latter is based on the large-scale description of the quantum dynamics and it differs from the mean-field prediction even in dimension larger than one. We moreover consider spatially inhomogeneous setups involving an external potential. In confined systems the density decay is accelerated towards the mean-field algebraic behavior, while for deconfined scenarios the power-law decay is replaced by a slower nonalgebraic decay.
Fungal Saprotrophic Promotion and Plant Pathogenic Suppression under Ditch-Buried Straw Return with Appropriate Burial Amount and Depth
2024-06-24

Fungi as heterotrophs are key participants in the decomposition of organic materials and the transformation of nutrients in agroecosystems. Ditch-buried straw return as a novel conservation management strategy can improve soil fertility and alter hydrothermal processes. However, how ditch-buried straw return strategies affect the soil fungal community is still unclear. Herein, a 7-year field trial was conducted to test the influences of burial depth (0, 10, 20, 30, and 40 cm) and the amount of ditch-buried straw (half, full, double) on the diversity, composition, and predicted functions of a soil fungal community, as well as the activities of carbon-degraded enzymes. Under the full amount of straw burial, the abundance of phylum Ascomycota was 7.5% higher as compared to other burial amount treatments. This further increased the activity of cellobiohydrolase by 32%, as revealed by the positive correlation between Ascomycota and cellobiohydrolase. With deeper straw burial, however, the abundance of Ascomycota and β-D-glucopyranoside activity decreased. Moreover, genus Alternaria and Fusarium increased while Mortierella decreased with straw burial amount and depth. FUNgild prediction showed that plant fungal pathogens were 1- to 2-fold higher, whilst arbuscular mycorrhizal fungi were 64% lower under straw buried with double the amount and at a depth of 40 cm. Collectively, these findings suggest that ditch-buried straw return with a full amount and buried at a depth less than 30 cm could improve soil nutrient cycles and health and may be beneficial to subsequent crop production.
Design, Synthesis, Antitumor, and Antiplasmodial Evaluation of New 7-Chloroquinoline–Benzimidazole Hybrids
2024-06-24

Newly synthesized 7-chloro-4-aminoquinoline–benzimidazole hybrids were characterized by NMR and elemental analysis. Compounds were tested for their effects on the growth of the non-tumor cell line MRC-5 (human fetal lung fibroblasts) and carcinoma (HeLa and CaCo-2), leukemia, and lymphoma (Hut78, THP-1, and HL-60) cell lines. The obtained results, expressed as the concentration at which 50% inhibition of cell growth is achieved (IC50 value), show that the tested compounds affect cell growth differently depending on the cell line and the applied dose (IC50 ranged from 0.2 to &gt;100 μM). Also, the antiplasmodial activity of these hybrids was evaluated against two P. falciparum strains (Pf3D7 and PfDd2). The tested compounds showed potent antiplasmodial activity, against both strains, at nanomolar concentrations. Quantitative structure–activity relationship (QSAR) analysis resulted in predictive models for antiplasmodial activity against the 3D7 strain (R2 = 0.886; Rext2 = 0.937; F = 41.589) and Dd2 strain (R2 = 0.859; Rext2 = 0.878; F = 32.525) of P. falciparum. QSAR models identified the structural features of these favorable effects on antiplasmodial activities.
Early occurrence of lion (Panthera spelaea) at the Middle Pleistocene Acheulean site of Notarchirico (MIS 16, Italy)
2024-06-24

Abstract The long sedimentary sequence of Notarchirico has yielded evidence of one of the earliest Acheulean manifestations in Europe and of recurrent hominin occupation, spanning from the end of the interglacial MIS 17 to the glacial MIS 16 (~695–610 ka). Here, we report the new discovery of a lion, Panthera spelaea , from the site, based on a metatarsal from layer A. This part of the sequence dates to ~660–612 ka (MIS 16, 40 Ar/ 39 Ar age). Therefore, Notarchirico's lion represents the earliest confirmed occurrence of the species in southwestern Europe, although older findings are known from adjacent areas. Lions and several other large mammal species dispersed into Europe during the Early–Middle Pleistocene Transition, which also witnessed the spread of the Acheulean. Ecological and behavioural adaptability was probably key, for hominins and other species, to cope with the intense and recurrent environmental fluctuations that occurred during this period.
Proactive reward in conflict tasks: Does it only enhance general performance or also modulate conflict effects?
2024-06-24

Abstract In the present study, we investigated the influence of performance-contingent reward prospects on task performance across three visual conflict tasks with manual responses (Experiments 1 &amp; 2: Simon and Stroop tasks; Experiment 3: Simon and Eriksen flanker task) using block-wise (Experiment 1) and trial-wise (Experiments 2 &amp; 3) manipulations to signal the possibility of reward. Across all experiments, task performance (in reaction time and/or error rates) generally improved in reward compared with no-reward conditions in each conflict task. However, there was, if any, little evidence that the reward manipulation modulated the size of the mean conflict effects, and there was also no evidence for conflict-specific effects of reward when controlling for time-varying fluctuations in conflict processing via distributional analyses (delta plots). Thus, the results provide no evidence for conflict-specific accounts and instead favor performance-general accounts, where reward anticipation leads to overall performance improvements without affecting conflict effects. We discuss possible implications for how proactive control might modulate the interplay between target- and distractor-processing in conflict tasks.
Coping with the COVID-19 Pandemic by Using Media: Extending the Coping Goodness-of-Fit Hypothesis to Media Use
2024-06-24

During the COVID-19 pandemic, people had to cope with stress in two different ways: engaging in problem-focused behaviors (e.g. seeking information) and regulating their emotions. In particular, during lockdowns, media played an important role in these coping processes. This short-term longitudinal study during the first stage of the COVID-19 pandemic in April 2020 in Germany (N = 348) tested how coping strategies related to two categories of coping effectiveness outcomes, situation-addressing outcomes (preventive behaviors and knowledge) and mental health outcomes (stress and perceived coping efficacy). We moreover tested which kind of media use is related to effective coping derived from a combination of the coping goodness-of-fit hypothesis and mood management theory. The study demonstrated that information seeking during the uncertain pandemic circumstances might have been a double-edged sword: Increased use of the coping strategy information seeking was related to increased stress but also to enhanced knowledge and better adherence to behavioral guidelines, however only on the cross-sectional level. Additionally, our results showed that using positive media content might have helped media users to reduce stress and to adhere to behavioral guidelines. Thus, a combination of seeking information at predefined, limited time points and using positive media content might have been a fruitful way of using media for coping during this situation of crisis.
Model matchmaking via the Solve-RD Rare Disease Models & Mechanisms Network (RDMM-Europe)
2024-06-24

In biomedical research, particularly for rare diseases (RDs), there is a critical need for model organisms to unravel the mechanistic basis of diseases, perform biomarker studies and develop potential therapeutic interventions. Within Solve-RD, an EU-funded research project with the aim of solving large numbers of previously unsolved RDs, the European Rare Disease Models &amp;amp; Mechanisms Network (RDMM-Europe) has been established.
MEK-inhibitor treatment reduces the induction of regulatory T cells in mice after influenza A virus infection
2024-06-24

Regulatory T cells (Tregs) play a crucial and complex role in balancing the immune response to viral infection. Primarily, they serve to regulate the immune response by limiting the expression of proinflammatory cytokines, reducing inflammation in infected tissue, and limiting virus-specific T cell responses. But excessive activity of Tregs can also be detrimental and hinder the ability to effectively clear viral infection, leading to prolonged disease and potential worsening of disease severity. Not much is known about the impact of Tregs during severe influenza. In the present study, we show that CD4 + /CD25 + FoxP3 + Tregs are strongly involved in disease progression during influenza A virus (IAV) infection in mice. By comparing sublethal with lethal dose infection in vivo , we found that not the viral load but an increased number of CD4 + /CD25 + FoxP3 + Tregs may impair the immune response by suppressing virus specific CD8 + T cells and favors disease progression. Moreover, the transfer of induced Tregs into mice with mild disease symptoms had a negative and prolonged effect on disease outcome, emphasizing their importance for pathogenesis. Furthermore, treatment with MEK-inhibitors resulted in a significant reduction of induced Tregs in vitro and in vivo and positively influenced the progression of the disease. Our results demonstrate that CD4 + /CD25 + FoxP3 + Tregs are involved in the pathogenesis of severe influenza and indicate the potential of the MEK-inhibitor zapnometinib to modulate CD4 + /CD25 + FoxP3 + Tregs. Thus, making MEK-inhibitors even more promising for the treatment of severe influenza virus infections.
Antimicrobial Evaluation of Two Polycyclic Polyprenylated Acylphloroglucinol Compounds: PPAP23 and PPAP53
2024-06-24

Polycyclic polyprenylated acylphloroglucinols (PPAPs) comprise a large group of compounds of mostly plant origin. The best-known compound is hyperforin from St. John&amp;#039;s wort with its antidepressant, antitumor and antimicrobial properties. Chemical synthesis of PPAP variants allows the generation of compounds with improved activity and compatibility. Here we studied two synthetic PPAP-derivatives, PPAP 23 and PPAP 53, as to their antimicrobial activity. While PPAP 23 is insoluble in water, PPAP 53, the sodium salt, is water-soluble. In vitro, both compounds exhibited good and comparable activity against methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus faecium. Both compounds had no adverse effects on Galleria mellonella wax moth larvae, even at high concentrations. However, they were unable to protect the larvae from infection with S. aureus because components of the larval coelom neutralized the antimicrobial activity; a similar neutralizing effect was also seen with serum albumin. In a septic arthritis model triggered by S. aureus PPAP23 decreased the formation of abscesses and bacterial load in kidneys, and in a mouse skin abscess model topical treatment with PPAP 53 resulted in an approximately two-fold reduction in S. aureus counts. PPAP 23 and PPAP 53 also showed high growth inhibiting activity against anaerobic Gram-positive pathogenic gut bacteria such as Clostridium perfringens and Clostridium difficile. Based on these results, we envision potential application of PPAP 23 and PPAP 53 in the topical treatment of wound infections with Gram+ pathogens, and their oral use in intestinal infections with C. difficile.
Ultrafast Brain MRI at 3 T for MS: Evaluation of a 51-Second Deep-Learning-Enhanced T2-EPI-FLAIR Sequence
2024-06-24

Magnetic resonance (MR) image acquisitions are usually time-consuming, limiting utilization in neuroimaging. For multiple sclerosis (MS) patients, MR imaging plays a major role in drug therapy decision-making. The purpose of this study was to evaluate whether an ultrafast, T2-weighted (T2w), deep-learning-enhanced (DL), echo-planar-imaging-based (EPI) fluid-attenuated inversion recovery (FLAIR) sequence (FLAIRUF) that has been targeting neurological emergencies so far might even be an option to detect MS lesions of the brain compared to conventional FLAIR sequences. Therefore, 17 MS patients were enrolled prospectively in this exploratory study. Standard MR protocols and ultrafast acquisitions were conducted at 3 tesla (T), including three-dimensional (3D)-FLAIR, turbo/fast spin echo (TSE)-FLAIR, and FLAIRUF. Inflammatory lesions were grouped by size and location. Lesion conspicuity and image quality were rated on an ordinal five-point Likert scale, and lesion detection rates were calculated. Statistical analyses were performed to compare results. Altogether, 568 different lesions were found. Data indicated no significant differences in lesion detection (sensitivity and positive predictive value [PPV]) between FLAIRUF and axially reconstructed 3D-FLAIR (lesion size ≥ 3 mm × ≥ 2 mm) and no differences in sensitivity between FLAIRUF and TSE-FLAIR (lesion size ≥ 3 mm total). Lesion conspicuity in FLAIRUF was similar in all brain regions except for superior conspicuity in the occipital lobe and inferior conspicuity in central brain regions. Further findings include location-dependent limitations of signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) as well as artifacts such as spatial distortions in FLAIRUF. In conclusion, FLAIRUF could potentially be an expedient alternative to conventional methods for brain imaging in MS patients since the acquisition can be performed in a fraction of time while maintaining good image quality.