julilili42/papers huggingface dataset:
Europium bis(dimethylsilyl)amides including mixed-valent Eu3[N(SiHMe2)2]6[μ-N(SiHMe2)2]2
2014-09-12

Trivalent Eu[N(SiHMe2)2]3(THF)2 can easily be synthesized by applying a routine salt metathesis protocol (EuCl3(THF)2 and 3 equiv. of Li[N(SiHMe2)2] in n-hexane) which crystallizes isotypically to its analogues of the rare-earth metal series (space group P21/c). Transsilylamination of Eu[N(SiMe3)2]2(THF)2 with a slight excess of HN(SiHMe2)2 in n-hexane-THF yields the divalent trinuclear compound Eu{[μ-N(SiHMe2)2]2Eu[N(SiHMe2)2](THF)}2, the solid-state structure of which differs significantly from the samarium and ytterbium analogues by showing three unique molecules in the asymmetric unit of which one is related to the two others by an inversion. Using crude Eu[N(SiMe3)2]3 in transsilylamination reactions with HN(SiHMe2)2 in n-hexane afforded n-hexane-insoluble trivalent ate complexes {MEu[N(SiHMe2)2]4}n (M = Na, K) depending on the synthesis conditions of Eu[N(SiMe3)2]3. Performing the transsilylamination of Eu[N(SiMe3)2]3 with a large excess of HN(SiHMe2)2 at elevated temperatures gave reproducibly the donor-free, mixed-valent, trinuclear compound Eu(II){[μ-N(SiHMe2)2]Eu(III)[N(SiHMe2)2]3}2 in good yield.
Brutalization Revisited: The Case of Russia
2014-09-12

In the case of Russia, the existence of a relationship between lost war, revolution, and Civil War is obvious. Unlike the German case, however, it has not been investigated in any detail. The Great War is often seen as the ‘forgotten war’ in the shadow of the Revolution of 1917 and the Civil War of 1918–21. Inspired by George Mosse’s work on Germany, this article investigates the connections between these conflicts. It argues that it is not easy to reconstruct the direct linkages between the fronts of an industrialized war to the violence of revolution and civil war. Rather, enduring traditions were transferred, transformed, and radicalized: the style of rule, the result of an absent political and patriotic consensus between the heterogeneous communities of communication, and dispositions to violence among the decisive sectors of the population, not only the soldiers and the revolutionaries, but also the peasants and workers longing for an egalitarian and just economy. The main role of the war was that it destroyed the old state and set these pre-existing violent tendencies free, which began to feed on themselves.
Direct Comparison of Targeted MRI-Guided Biopsy with Systematic Transrectal Ultrasound-Guided Biopsy in Patients with Previous Negative Prostate Biopsies
2014-09-13

&lt;b&gt;&lt;i&gt;Objective:&lt;/i&gt;&lt;/b&gt; To directly compare the diagnostic performance of targeted MRI-guided biopsy (MR-GB) and systematic transrectal ultrasound-guided biopsy (TRUS-GB). &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; Thirty-five patients with at least one negative TRUS-GB, persistently elevated or rising prostate-specific antigen and a lesion suspicious for prostate cancer (PC) on multiparametric MRI (mpMRI) scored by using the Prostate Imaging Reporting and Data System (PI-RADS) were included. A median of three targeted biopsies per lesion were obtained and systematic TRUS-GB was performed subsequently by an independent urologist without knowledge of the MRI findings. Definite pathology reports were analyzed for anatomical location and criteria of clinical significance. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; The tumor detection rate was significantly higher with MR-GB compared with TRUS-GB (16/35, 46% and 8/35, 23%, respectively, p &lt; 0.05). MR-GB detected PC in all patients with positive TRUS-GB. All tumors detected by MR-GB exhibited at least one criterion of clinical significance. PC lesions showed a significantly higher PI-RADS sum score compared with benign lesions. &lt;b&gt;&lt;i&gt;Conclusions:&lt;/i&gt;&lt;/b&gt; MR-GB is more effective compared with TRUS-GB in detecting clinically significant PC in men after previous negative TRUS-GB. PI-RADS scores give additional information and could be part of the decision-making process when considering retrial biopsy. Additional systematic biopsy can be omitted in patients undergoing targeted MR-GB.
Fc-Optimized NKG2D–Fc Constructs Induce NK Cell Antibody-Dependent Cellular Cytotoxicity against Breast Cancer Cells Independently of HER2/neu Expression Status
2014-09-13

Abstract The ability of NK cells to mediate Ab-dependent cellular cytotoxicity (ADCC) largely contributes to the clinical success of antitumor Abs, including trastuzumab, which is approved for the treatment of breast cancer with HER2/neu overexpression. Notably, only ∼25% of breast cancer patients overexpress HER2/neu. Moreover, HER2/neu is expressed on healthy cells, and trastuzumab application is associated with side effects. In contrast, the ligands of the activating immunoreceptor NKG2D (NKG2DL) are selectively expressed on malignant cells. In this study, we took advantage of the tumor-associated expression of NKG2DL by using them as target Ags for NKG2D-IgG1 fusion proteins optimized by amino acid exchange S239D/I332E in their Fc part. Compared to constructs with wild-type Fc parts, fusion proteins carrying the S239D/I332E modification (NKG2D–Fc–ADCC) mediated highly enhanced degranulation, ADCC, and IFN-γ production of NK cells in response to breast cancer cells. NKG2D–Fc–ADCC substantially enhanced NK reactivity also against HER2/neu-low targets that were unaffected by trastuzumab, as both compounds mediated their immunostimulatory effects in strict dependence of target Ag expression levels. Thus, in line with the hierarchically organized potential of the various activating receptors governing NK reactivity and due to its highly increased affinity to CD16, NKG2D–Fc–ADCC potently enhances NK cell reactivity despite the inevitable reduction of activating signals upon binding to NKG2DL. Due to the tumor-restricted expression of NKG2DL, NKG2D–Fc–ADCC may constitute an attractive means for immunotherapy especially of HER2/neu-low or -negative breast cancer.
Twinship, incest, and twincest in the Harry Potter universe
2014-09-15

Among the large group of Harry Potter fans who write their own stories about the boy wizard, his friends, and his foes, and publish them on the Internet, some are interested in the exploration of the erotic and romantic bond between identical twins. Because the Harry Potter saga features two sets of identical twin pairs of different gender—the Weasley brothers and the Patil sisters—the series not only provides a unique playground for the recipients in terms of the possibilities for twincest stories; more importantly, it offers ample opportunity for researchers to examine how fans actually use such pairings. In this essay, the examination of twin relationships as portrayed within Rowling's works, the movies, and in twincest fan fiction are confronted with each other to outline how Rowling's different concepts of the sibling pairs and the author's general ongoing interest in doubling motifs is consistently expanded by fan fiction writers to discuss the complex relationship between source and fan text.
The big-fish-little-pond effect: Generalizability of social comparison processes over two age cohorts from Western, Asian, and Middle Eastern Islamic countries.
2014-09-15

Extensive support for the seemingly paradoxical negative effects of school- and class-average achievement on academic self-concept (ASC)—the big-fish-little-pond effect (BFLPE)—is based largely on secondary students in Western countries or on cross-cultural Program for International Student Assessment studies. There is little research testing the generalizability of this frame of reference effect based on social comparison theory to primary school students and or to matched samples of primary and secondary students from different countries. Using multigroup–multilevel latent variable models, we found support for developmental and cross-cultural generalizability of the BFLPE based on Trends in International Mathematics and Science Study data; positive effects of individual student achievement and the negative effects of class-average achievement on ASC were significant for each of the 26 groups (nationally representative samples of 4th- and 8th-grade students from 13 diverse countries; 117,321 students from 6,499 classes).
Novel insights into the molecular pathogenesis ofCYP4V2‐associated Bietti's retinal dystrophy
2014-09-15

Bietti's crystalline dystrophy (BCD) is a rare, autosomal recessive retinal degenerative disease associated with mutations in CYP4V2. In this study, we describe the genetic and clinical findings in 19 unrelated BCD patients recruited from five international retinal dystrophy clinics. Patients underwent ophthalmic examinations and were screened for CYP4V2 mutations by Sanger sequencing and quantitative polymerase chain reaction (qPCR) copy number variation screening. Eight CYP4V2 mutations were found in 10/19 patients, including three patients in whom only monoallelic mutations were detected. Four novel mutations were identified: c.604G>A; p.(Glu202Lys), c.242C>G; p.(Thr81Arg), c.604+4A>G; p.(?), and c.1249dup; p.(Thr417Asnfs*2). In addition, we identified a heterozygous paternally inherited genomic deletion of at least 3.8 Mb, encompassing the complete CYP4V2 gene and several other genes, which is novel. Clinically, patients demonstrated phenotypic variability, predominantly showing choroidal sclerosis, attenuated vessels, and crystalline deposits of varying degrees of severity. To our knowledge, our study reports the first heterozygous CYP4V2 deletion and hence a novel mutational mechanism underlying BCD. Our results emphasize the importance of copy number screening in BCD. Finally, the identification of CYP4V2-negative patients with indistinguishable phenotypes from CYP4V2-positive patients might suggest the presence of mutations outside the coding regions of CYP4V2, or locus heterogeneity, which is unreported so far.
A Membrane‐Bound Prenyltransferase Catalyzes the O‐Prenylation of 1,6‐Dihydroxyphenazine in the Marine Bacterium Streptomyces sp. CNQ‐509
2014-09-15

Abstract Streptomyces sp. CNQ‐509 produces the rare O‐prenylated phenazines marinophenazines A and B. To identify the enzyme catalyzing the O‐prenyl transfer in marinophenazine biosynthesis, we sequenced the genome of S. sp. CNQ‐509. This led to the identification of two genomic loci harboring putative phenazine biosynthesis genes. The first locus contains orthologues for all seven genes involved in phenazine‐1‐carboxylic acid biosynthesis in pseudomonads. The second locus contains two known phenazine biosynthesis genes and a putative prenyltransferase gene termed cnqPT1. cnqPT1 codes for a membrane protein with sequence similarity to the prenyltransferase UbiA of ubiquinone biosynthesis. The enzyme CnqPT1 was identified as a 1,6‐dihydroxyphenazine geranyltransferase, which catalyzes the CO bond formation between C‐1 of the geranyl moiety and O‐6 of the phenazine scaffold. CnqPT1 is the first example of a prenyltransferase catalyzing O‐prenyl transfer to a phenazine.
The behavioural economics of competitive balance: implications for league policy and championship management
2014-09-15

Abstract:&#13;\\n\\t&#13;\\nThe theory of competitive balance represents one of the core concepts of sports economics. Based upon an international research project analyzing the perception of competitive balance by consumers (Pawlowski 2013a, 2013b; Pawlowski &amp; Budzinski 2013, 2014), we argue in this paper that behavioural explanations of competitive balance may offer additional insights for selected sports economics and, in particular, sports policy problems, complementing the standard view on competitive balance. After summarizing the standard analysis of competitive balance in sports economics concerning theory, policy and empirical record in chapter 2, we report the main theoretical and empirical insights from our research project (chapter 3, closely drawing on the respective publications). In addition to providing a more comprehensive picture of the behavioural economics of competitive balance, we add a discussion of sports policy implications (chapter 4). While perceived competitive balance is found to matter, there are rather narrow conditions for sports policy interventions or restrictive regulations of competition by the league management (or sports associations). Furthermore, it is not the balance of the overall league that matters. Instead, it is sufficient or even advantageous if the most relevant subcompetitions, like the race for the championship or the fight against relegation, are balanced among a narrow oligopoly of contenders.
Phenotypic and Genotypic Characterization of Daptomycin-Resistant Methicillin-Resistant Staphylococcus aureus Strains: Relative Roles of mprF and dlt Operons
2014-09-16

Development of in vivo daptomycin resistance (DAP-R) among Staphylococcus aureus clinical isolates, in association with clinical treatment failures, has become a major therapeutic problem. This issue is especially relevant to methicillin-resistant S. aureus (MRSA) strains in the context of invasive endovascular infections. In the current study, we used three well-characterized and clinically-derived DAP-susceptible (DAP-S) vs. resistant (DAP-R) MRSA strain-pairs to elucidate potential genotypic mechanisms of the DAP-R phenotype. In comparison to the DAP-S parental strains, DAP-R isolates demonstrated (i) altered expression of two key determinants of net positive surface charge, either during exponential or stationary growth phases (i.e., dysregulation of dltA and mprF), (ii) a significant increase in the D-alanylated wall teichoic acid (WTA) content in DAP-R strains, reflecting DltA gain-in-function; (iii) heightened elaboration of lysinylated-phosphatidylglyderol (L-PG) in DAP-R strains, reflecting MprF gain-in-function; (iv) increased cell membrane (CM) fluidity, and (v) significantly reduced susceptibility to prototypic cationic host defense peptides of platelet and leukocyte origins. In the tested DAP-R strains, genes conferring positive surface charge were dysregulated, and their functionality altered. However, there were no correlations between relative surface positive charge or cell wall thickness and the observed DAP-R phenotype. Thus, charge repulsion mechanisms via altered surface charge may not be sufficient to explain the DAP-R outcome. Instead, changes in the compositional or biophysical order of the DAP CM target of such DAP-R strains (i.e., increased fluidity) may be essential to this phenotype. Taken together, DAP-R in S. aureus appears to involve multi-factorial and strain-specific adaptive mechanisms.
Century-scale methylome stability in a recently diverged Arabidopsis thaliana lineage
2014-09-16

There has been much excitement about the possibility that exposure to specific environments can induce an ecological memory in the form of whole-sale, genome-wide epigenetic changes that are maintained over many generations. In the model plant Arabidopsis thaliana, numerous heritable DNA methylation differences have been identified in greenhouse-grown isogenic lines, but it remains unknown how natural, highly variable environments affect the rate and spectrum of such changes. Here we present detailed methylome analyses in a geographically dispersed A. thaliana population that constitutes a collection of near-isogenic lines, diverged for at least a century from a common ancestor. We observed little DNA methylation divergence whole-genome wide. Nonetheless, methylome variation largely reflected genetic distance, and was in many aspects similar to that of lines raised in uniform conditions. Thus, even when plants are grown in varying and diverse natural sites, genome-wide epigenetic variation accumulates in a clock-like manner, and epigenetic divergence thus parallels the pattern of genome-wide DNA sequence divergence.
The Influence of a Single Transition Metal Atom on the Reactivity of Main Group Metal Clusters in the Gas Phase
2014-09-16

Abstract Collision induced dissociation experiments of the metalloid clusters [(CO) 5 CrGe 9 R 3 ] – 2 and [(CO) 3 CrGe 9 R 3 ] – 3 are presented, showing that 2 can lose Cr(CO) 5 or CO to give [Ge 9 R 3 ] – 1 or 3 , respectively. Further dissociation from 3 leads first of all to the metalloid cluster [CrGe 9 R 3 ] – 4 , from which different elimination routes start. Thereby significant differences with respect to the chromium free cluster [Ge 9 R 3 ] – 1 are observed, where beside C–H also Si–C and Si–Si bond dissociation at low threshold energy take place, showing that 4 , with its additional chromium atom, is an ideal model system for a single atom catalyst.
Visual exploration of dirty activity sensor and emotional state data from psychological experiments
2014-09-16

We present a system for visual exploration of time-dependent activity sensor and emotion state data gained from psychological experiments. The real data has several quality problems such as missing or implausible observations as well as data inconsistencies. Therefore, our system automatically pre-processes the data. It then shows the results in an interactive interface. Two interactive views allow for gaining insights into children's sleep time patterns, activity during sleep and emotional changes over multiple nights. We developed and tested the system together with psychological experts. We show a use case applying our system to real-world data gained in a children study.
Cardiovascular magnetization transfer ratio imaging compared with histology: A postmortem study
2014-09-16

Cardiovascular magnetization transfer ratio (MTR) imaging by steady state free precession is a promising imaging method to assess microstructural changes within the myocardium. Hence, MTR imaging was correlated to histological analysis. Three postmortem cases were selected based on a suspicion of myocardial infarction. MTR and T 2 ‐weighted ( T 2w ) imaging was performed, followed by autopsy and histological analysis. All tissue abnormalities, identified by autopsy or histology, were retrospectively selected on visually matched MTR and T 2w images, and corresponding MTR values compared with normal appearing tissue. Regions of elevated MTR (up to approximately 20%, as compared to normal tissue), appearing hypo‐intense in T 2w ‐images, revealed the presence of fibrous tissue in microscopic histological analysis. Macroscopic observation (autopsy) described scar tissue only in one case. Regions of reduced MTR (up to approximately 20%) corresponded either to (i) the presence of edema, appearing hyperintense in T 2w ‐images and confirmed by autopsy, or to (ii) inflammatory granulocyte infiltration at a microscopic level, appearing as hypo‐intense T 2w ‐signal, but not observed by autopsy. Findings from cardiovascular MTR imaging corresponded to histology results. In contrast to T 2w ‐imaging, MTR imaging discriminated between normal myocardium, scar tissue and regions of acute myocardial infarction in all three cases. J. Magn. Reson. Imaging 2014;40:915–919 . © 2013 Wiley Periodicals, Inc.
Two Distinct Interneuron Circuits in Human Motor Cortex Are Linked to Different Subsets of Physiological and Behavioral Plasticity
2014-09-17

How does a single brain region participate in multiple behaviors? Here we argue that two separate interneuron circuits in the primary motor cortex (M1) contribute differently to two varieties of physiological and behavioral plasticity. To test this in human brain noninvasively, we used transcranial magnetic stimulation (TMS) of M1 hand area to activate two independent sets of synaptic inputs to corticospinal neurons by changing the direction of current induced in the brain: posterior-to-anterior current (PA inputs) and anterior-to-posterior current (AP inputs). We demonstrate that excitability changes produced by repetitive activation of AP inputs depend on cerebellar activity and selectively alter model-based motor learning. In contrast, the changes observed with repetitive stimulation of PA inputs are independent of cerebellar activity and specifically modulate model-free motor learning. The findings are highly suggestive that separate circuits in M1 subserve different forms of motor learning.
Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues
2014-09-17

Bacteria possess protein serine/threonine and tyrosine kinases which resemble eukaryal kinases in their capacity to phosphorylate multiple substrates. We hypothesized that the analogy might extend further, and bacterial kinases may also undergo mutual phosphorylation and activation, which is currently considered as a hallmark of eukaryal kinase networks. In order to test this hypothesis, we explored the capacity of all members of four different classes of serine/threonine and tyrosine kinases present in the firmicute model organism Bacillus subtilis to phosphorylate each other in vitro and interact with each other in vivo. The interactomics data suggested a high degree of connectivity among all types of kinases, while phosphorylation assays revealed equally wide-spread cross-phosphorylation events. Our findings suggest that the Hanks-type kinases PrkC, PrkD, and YabT exhibit the highest capacity to phosphorylate other B. subtilis kinases, while the BY-kinase PtkA and the two-component-like kinases RsbW and SpoIIAB show the highest propensity to be phosphorylated by other kinases. Analysis of phosphorylated residues on several selected recipient kinases suggests that most cross-phosphorylation events concern key regulatory residues. Therefore, cross-phosphorylation events are very likely to influence the capacity of recipient kinases to phosphorylate substrates downstream in the signal transduction cascade. We therefore conclude that bacterial serine/threonine and tyrosine kinases probably engage in a network-type behavior previously described only in eukaryal cells.
Utility and Diagnostic Pitfalls of SOX11 Monoclonal Antibodies in Mantle Cell Lymphoma and Other Lymphoproliferative Disorders
2014-09-17

Mantle cell lymphoma (MCL) is classically characterized by t(11;14) leading to cyclin D1 overexpression. Recently the transcription factor SOX11 has been discovered to be expressed in most MCL, including cyclin D1-negative cases. In this study we assess the performance of 2 commercially available monoclonal antibodies, Atlas Antibodies (Stockholm, Sweden) clone CLO142 and Cell Marque (Rocklin, CA) clone MRQ-58, for SOX11 immunohistochemistry in MCL, both cyclin D1 positive and cyclin D1 negative, as well as in cases of other small B-cell lymphoproliferative disorders, diffuse large B-cell lymphomas (DLBCLs), Burkitt lymphomas, and lymphoblastic leukemia/lymphomas. We also performed Western blots to further characterize the antibody specificity. Both antibodies show reliable, clear nuclear staining in MCL with variable specificity. However, the MRQ-58 antibody was more specific for MCL than CLO142, which showed considerably more nonspecific staining, especially in DLBCLs (59% positive vs. 4% positive with MRQ-58). In addition we reconfirmed the utility of SOX11 IHC for identifying cases of cyclin D1-negative blastoid MCL. However, we also identified cases of SOX11-positive DLBCL and splenic marginal zone lymphoma. Although SOX11 IHC is a powerful, and relatively accessible, tool to identify MCLs with variant immunophenotypes and/or morphology, these latter 2 cases highlight the need for strict criteria for interpreting SOX11 staining.