PD8-10 URETHROPLASTY OF INDUCED URETHRAL STRICTURE WITH SEEDED COLLAGEN TYPE I CELL CARRIERS IN LONG-TERM MINIPIGS
2014-03-28

You have accessJournal of UrologyStem Cell Research1 Apr 2014PD8-10 URETHROPLASTY OF INDUCED URETHRAL STRICTURE WITH SEEDED COLLAGEN TYPE I CELL CARRIERS IN LONG-TERM MINIPIGS Lisa Daum, Martin Vaegler, Sabine Maurer, Jens Mundhenk, Bastian Amend, and Karl-Dietrich Sievert Lisa DaumLisa Daum More articles by this author , Martin VaeglerMartin Vaegler More articles by this author , Sabine MaurerSabine Maurer More articles by this author , Jens MundhenkJens Mundhenk More articles by this author , Bastian AmendBastian Amend More articles by this author , and Karl-Dietrich SievertKarl-Dietrich Sievert More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.804AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Autologous matrix-stabilized urothelium offers an interesting therapeutic option for lower urinary tract reconstruction, particularly for the treatment of long and complicated urethral strictures. Previous studies showed that collagen matrices increase the stability of cell-based implants and reveal excellent in vivo biocompatibility. In this study, collagen type I cell carriers (CCC) were seeded with autologous urothelial cells and evaluated for urethroplasty in minipigs over a period of six months. METHODS Urethral strictures were induced by thermocoagulation in eight minipigs together with bladder tissue harvesting for isolation of autologous porcine urothelial cells(PUC). PUC were expanded, labelled by PKH26, seeded onto CCC in high density prior to induction of stratification. Three weeks after stricture induction urethroplasty was performed in all minipigs with urothelium matrix constructs. After six months, urethral tissues were extracted and examined histologically. Pancytokeratin, CK-20, and p63 served to confirm urothelial differentiation while E-Cadherin and ZO-1 verified the presence of intact cell-cell junctions. Urethrographic analysis was also performed to assess the integrity of the urethra before and after urethroplasty and potential stricture recurrence. RESULTS All animals tolerated the required interventions and recovered quickly without clinical impairment. The CCC was characterized by excellent stability and suturability when manipulated and applied during the surgery procedure. Urothelium matrix constructs integrated well into the host tissue without inflammation. None of the animals showed symptoms of rejection or stricture recurrence. In all tissue sections of the minipigs, transplanted cells could be detected via PKH26 fluorescence, although it diminished after six months. Immunofluorescence analysis confirmed the epithelial phenotype, junction formation and ongoing differentiation two weeks post-op. Contrast x-ray imaging did not reveal any differences in urethral constitution prior to and after application of the seeded matrices. CONCLUSIONS The study demonstrated that urethroplasty in a large animal model using CCC seeded with autologous PUC was feasible. Neither short-term nor long-term complications like stricture recurrence appeared. Confirmed characteristics of CCC such as excellent suitability and biodegradability were noted and the application of this industrial manufactured collagen type I matrix for urologic reconstruction purposes can be recommended for clinical use. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e220 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Lisa Daum More articles by this author Martin Vaegler More articles by this author Sabine Maurer More articles by this author Jens Mundhenk More articles by this author Bastian Amend More articles by this author Karl-Dietrich Sievert More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...