julilili42/papers huggingface dataset:
Alterations in connexin expression in the bladder of patients with urge symptoms
2005-08-11

OBJECTIVE To compare the formation of gap junctions between detrusor smooth muscle cells in situ and the distribution of connexin (Cx)40, Cx43 and Cx45 expressions in bladder biopsies from a control group (with bladder tumour) and from patients with urge symptoms, as smooth muscle cells of the human detrusor muscle communicate via gap junctions and express several connexin subtypes, alterations of which may be involved in the causes of lower urinary tract symptoms. MATERIALS AND METHODS Connexin expression is prominent in myofibroblast‐like cells, supposedly involved in afferent signalling pathways of the bladder. Their strategic position directly beneath the urothelium suggests they are a link between urothelial ATP signalling during bladder filling and afferent Aδ‐fibre stimulation for co‐ordination of bladder tonus and initialization of the micturition reflex. Modification of their coupling characteristics may have profound impact on bladder sensation. Bladder tissue probes of patients undergoing cystectomy or transurethral tumour resection for bladder cancer were used as controls. Tissue samples from patients with severe idiopathic urge symptoms were taken for exclusion diagnostics of interstitial cystitis (IC) and carcinoma in situ . The formation of functional syncytia between detrusor smooth muscle cells were examined in dye‐coupling experiments by injecting with Lucifer Yellow. The morphology and structure of gap junctions were assessed by transmission electron microscopy and immunogold labelling of Cx43 and Cx45. The expression of connexin subtypes Cx40, Cx43 and Cx45 was compared by indirect immunofluorescence, and confocal laser scanning microscopy used for semiquantitative analysis. RESULTS There was dye coupling between smooth muscle cells of the detrusor in situ . Electron microscopy and immunogold labelling showed very small gap junctional plaques. These findings were confirmed by confocal immunofluorescence. Semiquantitative analyses showed significantly higher Cx43 expression in the detrusor muscle, and a tendency to higher Cx45 expression in the suburothelial layer associated with urge symptoms, whereas Cx40 expression was unaffected. CONCLUSIONS Smooth muscle cells of the human detrusor muscle are coupled by classical gap junctions, forming limited local functional syncytia. Both Cx43 and Cx45 are expressed at low levels in normal detrusor. Up‐regulation of Cx43 in patients with urge incontinence supports the possibility of functional changes in the syncytial properties of detrusor smooth muscle cells in this condition. In addition, the observed increase of Cx45 in the myofibroblast cell layer supports the idea that alterations in sensory signalling are also involved. Comparison with previous reports implies that the pathophysiology of urgency is distinct from that of the unstable bladder and other forms of incontinence.
Identification of classical swine fever virus protein E2 as a target for cytotoxic T cells by using mRNA-transfected antigen-presenting cells
2005-08-12

Vaccination of pigs against Classical swine fever virus (CSFV) by using live-virus vaccines induces early protection before detectable humoral immune responses. Immunological analyses indicate that this is associated with T-cell activation, underlining the importance of targeting cytotoxic T-lymphocyte (CTL) responses for vaccine improvement. Antigen-presenting cells (APCs) transfected with mRNA encoding structural protein E2 or non-structural viral proteins NS3–NS4A were used to identify viral genes encoding CTL epitopes. Monocyte-derived dendritic cells (DCs) and fibrocytes served as the APCs. In vitro translation of the mRNA and microscopic analysis of transfected cells demonstrated that E2 and NS3–NS4A could be identified. APCs transfected with either of the mRNA molecules restimulated CSFV-specific T cells to produce gamma interferon and specific cytotoxic activity against CSFV-infected target cells. The presence of CTL epitopes on E2 was confirmed by using d/d-haplotype MAX cells expressing E2 constitutively as target cells in d/d-haplotype CTL assays. A potent CTL activity against E2 was detected early (1–3 weeks) after CSFV challenge. This work corroborates the existence of CTL epitopes within the non-structural protein domain NS3–NS4A of CSFV. Furthermore, epitopes on the E2 protein can also now be classified as targets for CTLs, having important implications for vaccine design, especially subunit vaccines. As for the use of mRNA-transfected APCs, this represents a simple and efficient method to identify viral genes encoding CTL epitopes in outbred populations.
Symmetry-projected variational approach to the one-dimensional Hubbard model
2005-08-12

We apply a variational method devised for the nuclear many--body problem to the 1-dimensional Hubbard--model with nearest neighbor hopping and periodic boundary conditions. The test wave function consist for each state out of a single Hartree--Fock determinant mixing all the sites (or momenta) as well as the spin--projections of the electrons. Total spin and linear momentum are restored by projection methods before the variation. It is demonstrated that this approach reproduces the results of exact diagonalisations for half--filled N=12 and N=14 lattices not only for the energies and occupation numbers of the ground but also of the lowest excited states rather well. Furthermore, a system of 10 electrons in a N=12 lattice is investigated and, finally, a N=30 lattice is studied. In addition to energies and occupation numbers we present the spectral functions computed with the help of the symmetry--projected wave functions, too. Also here nice agreement with the exact results (where available) is reached.
Chemokines in onchocerciasis patients after a single dose of ivermectin
2005-08-12

Summary Ivermectin treatment will effectively diminish microfilariae (Mf) of Onchocerca volvulus in the skin of patients, but therapy is associated with adverse host inflammatory responses. To investigate the association of proinflammatory chemokines with the intensity of infection and clinical adverse reactions, chemokine serum levels were measured in patients following ivermectin treatment (100 μg/kg, 150 μg/kg or 200 μg/kg) or placebo. The density of O. volvulus Mf per mg skin decreased by 85%, 97%, 97% and 90% at day 3, at month 3, month 6 and at 1 year post-ivermectin. The cutaneous T cell-attracting chemokine (CTACK/CCL27) was found highly elevated in onchocerciasis patients compared to infection-free European controls (P = 0·0004) and it did not change following ivermectin or placebo to 1 year post-therapy. The chemokine RANTES/CCL5 (regulated on activated and normally T cell-expressed) was similarly high in onchocerciasis patients and infection-free European controls; the RANTES/CCL5 levels did not change following treatment until 6 months post-therapy but were slightly elevated at 1 year post-therapy (P &amp;lt; 0·02). In contrast, the Th2-type chemoattractants, thymus and activation regulated chemokine (TARC/CCL17) and macrophage-derived chemokine (MDC/CCL22), were activated at 3 days post-ivermectin (P &amp;lt; 0·0001) to return to pretreatment or lower levels thereafter. The Th1-type chemoattractants, macrophage inflammatory protein (MIP)-1α/CCL3 and MIP-1β/CCL4 were low before ivermectin treatment, but following clearance of microfilariae of O. volvulus their levels increased from 6 months post-therapy onwards (for both at 12 months post-therapy, P &amp;lt; 0·0001). The adverse reaction scores (RS) in treated patients increased significantly on day 3 (P &amp;lt; 0·02) while it remained unchanged in those who received placebo (P = 0·22); RS interacted with the microfilarial density (P = 0·01), but not with the dose of ivermectin or with the serum levels of MIP-1α/CCL3, MIP-1β/CCL4, TARC/CCL17, MDC/CCL22 and CTACK/CCL27. Our observations suggest that following ivermectin, macrophages as well as memory Th2-type lymphocytes and B cells, attracted and activated by MDC/CCL22, TARC/CCL17 and CTACK/CCL27, may contribute to dermal immune responses and O. volvulus Mf killing and clearance. The transient changes of TARC/CCL17 and MDC/CCL22 were not associated with clinical adverse responses, and the later rise of MIP-1α/CCL3 and MIP-1β/CCL4 showed a reactivation of Type 1 immune responses associated with persistent low levels of O. volvulus microfilariae and an expiring O. volvulus infection.
High energy phosphates in postischemic myocardium with reduced or normalized function
2005-08-13

Mitochondrial damage and decreased levels of high energy phosphates are well-known findings in reversibly injured postischemic myocardium (=stunning). Using an isovolumetrically beating isolated rat heart preparation (Langendorff perfusion apparatus) we investigated the effect of postischemic positive inotropic stimulation on the myocardial high energy phosphate content. During postischemic functional steady state with moderately reduced left ventricular developed pressure (118.7 mmHg after 30 minutes of ischemia vs 136.1 mmHg preischemic), dopamine in a dose to reestablish preischemic function was administered for a 20-minute period. Data were compared with postischemic hearts without positive inotropic stimulation: ATP was 2.5 vs 2.5 (μmol/g ww; phosphocreatine was 5.5 vs 6.7 μmol/g ww. These findings indicate that reversibly injured postischemic myocardium can be stimulated for a prolonged period without loss of high-energy phosphates. Therefore, a failure of the energy-generating apparatus is not the reason for the reversible postischemic dysfunction.
Activation of Vγ9Vδ2 T Cells by NKG2D
2005-08-15

Abstract Human Vγ9Vδ2 T cells recognize phosphorylated nonpeptide Ags (so called phosphoantigens), certain tumor cells, and cells treated with aminobisphosphonates. NKG2D, an activating receptor for NK cells, has been described as a potent costimulatory receptor in the Ag-specific activation of γδ and CD8 T cells. This study provides evidence that Vγ9Vδ2 T cells may also be directly activated by NKG2D. Culture of PBMC with immobilized NKG2D-specific mAb or NKG2D ligand MHC class I related protein A (MICA) induces the up-regulation of CD69 and CD25 in NK and Vγ9Vδ2 but not in CD8 T cells. Furthermore, NKG2D triggers the production of TNF-α but not of IFN-γ, as well as the release of cytolytic granules by Vγ9Vδ2 T cells. Purified Vγ9Vδ2 T cells kill MICA-transfected RMA mouse cells but not control cells. Finally, DAP10, which mediates NKG2D signaling in human NK cells, was detected in resting and activated Vγ9Vδ2 T cells. These remarkable similarities in NKG2D function in NK and Vγ9Vδ2 T cells may open new perspectives for Vγ9Vδ2 T cell-based immunotherapy, e.g., by Ag-independent killing of NKG2D ligand-expressing tumors.
Stylized augmented reality for improved immersion
2005-08-16

The ultimate goal of augmented reality is to provide the user with a view of the surroundings enriched by virtual objects. Practically all augmented reality systems rely on standard real-time rendering methods for generating the images of virtual scene elements. Although such conventional computer graphics algorithms are fast, they often fail to produce sufficiently realistic renderings. The use of simple lighting and shading methods, as well as the lack of knowledge about actual lighting conditions in the real surroundings, cause virtual objects to appear artificial. In this paper, we propose an entirely novel approach for generating augmented reality images in video see-through systems. Our method is based on the idea of applying stylization techniques for reducing the visual realism of both the camera image and the virtual graphical objects. A special painterly image filter is applied to the camera video stream. The virtual scene elements are generated using a non-photorealistic rendering method. Since both the camera image and the virtual objects are stylized in a corresponding "cartoon-like" or "sketch-like" way, they appear very similar. As a result, the graphical objects seem to be an actual part of the real surroundings. We describe both the new painterly filter for the camera image and the non-photorealistic rendering method for virtual scene elements, which has been adapted for this purpose. Both are fast enough for generating augmented reality images in real-time and are highly customizable. The results obtained using our method, are very promising and show that it improves immersion in augmented reality.
Investigations on the mechanism of silicon etching with chlorine-trifluoride
2005-08-16

We investigated chlorine trifluoride (ClF3) etching of silicon with a patterned oxide mask layer prepared by e-beam lithography. The mask apertures varied from 0.1μmto300μm. This enables to adjust the flow rate of ClF3 molecules into the etched cavity leading to a strong variation of the ClF3 abundance at the silicon surface. A crucial dependence of the etch rate on the aperture area was observed revealing a maximum of the etch rate for a specific ClF3 abundance. A physical description of the etch process is developed in order to distinguish between different mechanisms within the etching process. At low ClF3 abundance the etch rate is limited due to a lack of ClF3. For high abundance the etch rate is assumed to be hampered by a diffusion like transport of ClF3 molecules through a fluorosilyl layer formed on the silicon surface. It can be shown that the etch rate of silicon with ClF3 is not limited by the chemical reaction at high ClF3 abundance. Additionally, we observed a change in etching behavior from isotropic to anisotropic with a strong correlation to the etching regime.
PPAR-γ agonists inhibit toll-like receptor-mediated activation of dendritic cells via the MAP kinase and NF-κB pathways
2005-08-17

Dendritic cells (DCs) play an important role in initiating and maintaining primary immune responses. However, mechanisms involved in the resolution of these responses are elusive. We analyzed the effects of 15d-PGJ2 and the synthetic peroxisome proliferator-activated receptor (PPAR)-γ ligand troglitazone (TGZ) on the immunogenicity of human monocyte-derived DCs upon stimulation with toll-like receptor (TLR) ligands. Activation of PPAR-γ resulted in a reduced stimulation of DCs via the TLR ligands 2, 3, 4, and 7, characterized by down-regulation of costimulatory and adhesion molecules and reduced secretion of cytokines and chemokines involved in T-lymphocyte activation and recruitment. MCP-1 (monocyte chemotactic protein-1) production was increased due to PPAR-γ activation. Furthermore, TGZ-treated DCs showed a significantly reduced capacity to stimulate T-cell proliferation, emphasizing the inhibitory effect of PPAR-γ activation on TLR-induced DC maturation. Western blot analyses revealed that these inhibitory effects on TLR-induced DC activation were mediated via inhibition of the NF-κB and mitogen-activated protein (MAP) kinase pathways while not affecting the PI3 kinase/Akt signaling. Our data demonstrate that inhibition of the MAP kinase and NF-κB pathways is critically involved in the regulation of TLR and PPAR-γ-mediated signaling in DCs.
Influence of general self‐efficacy on the effects of a school‐based universal primary prevention program of depressive symptoms in adolescents: a randomized and controlled follow‐up study
2005-08-17

Background: Depressive disorders in adolescents are a widespread and increasing problem. Prevention seems a promising and feasible approach. Methods: We designed a cognitive‐behavioral school‐based universal primary prevention program and followed 347 eighth‐grade students participating in a randomized controlled trial for three months. Results: In line with our hypothesis, participants in the prevention program remained on a low level of depressive symptoms, having strong social networks. The control group showed increasing depressive symptoms and a reduced social network. Contrary to our expectations, students low in self‐efficacy benefited more from the program than high self‐efficient students. Social network did not mediate the relationship between participation in the prevention program and changes in depressive symptoms. Conclusions: Our results show that the prevention program had favorable effects. Further research is needed to explore the impact of self‐efficacy on the effects of prevention programs.
In vitro study on the immunological effect of bromelain and trypsin on mononuclear cells from humans.
2005-08-17

These results indicate that bromelain - but not trypsin - activates macrophages/monocytes and type 1 cells independently from the underlying disease and may stimulate, therefore, the innate as well as the adaptive immune system.
First Generation and Trapping of a Dehydrometallophthalocyanine Starting from Triazole‐Functionalized Zinc Phthalocyanine
2005-08-17

Abstract Direct 1 N ‐amination of the triazole‐fused zinc phthalocyanine 2 and oxidation of the formed amino derivative 3 resulted in the generation of the very reactive intermediate, the dehydrometallophthalocyanine 4 , which was not known previously. The latter was trapped in situ with different dienes, for example, furan, tetraphenylcyclopentadienone, and anthracene to form the corresponding Diels–Alder adducts. The products were characterized by 1 H and 13 C‐dept135 NMR, and UV/Vis spectroscopy, MALDI‐TOF mass spectrometry, and elemental analysis, which are fully in agreement with their structure. The developed synthetic procedure opens a simple and versatile pathway towards unsymmetrical peripheral modification of phthalocyanines, which is readily applicable to the micromol scale and is important for the design of new interesting Pc‐based systems.
Spatial orientation in echolocating harbour porpoises (Phocoena phocoena)
2005-08-18

SUMMARY Studies concerning the echolocation behaviour of odontocetes focus mainly on target detection and discrimination, either in stationary animals or in animals approaching a specific target. We present the first data on the use of echolocation for spatial orientation or navigation. Synchronised video and high-frequency recordings were made of two harbour porpoises trained to swim from one position to another across an outdoor pool in order to correlate swimming and echolocation behaviour. Both porpoises showed a clear range-locking behaviour on specific positions near the end of the pool, as indicated by a decrease in click interval with decreasing distance. The decrease in click interval followed the two-way-transit time, which is the time interval between the outgoing click and the received echo from the focal object. This suggests that the porpoises used focal objects as landmarks. The lag time, defined as the time between the arrival of an echo from a landmark and the emission of the next click, was task specific. The lag time was longer for difficult tasks (26–36 ms) and shorter for simpler tasks(14–19 ms), with some individual differences between the two animals. Our results suggest that echolocation by odontocetes is used not only for target detection, localisation and classification but also for spatial orientation.
Quantitative analysis of lung and tumour mobility: comparison of two time-resolved MRI sequences
2005-08-18

The purpose of this study was to describe the use of parallel imaging technique (PAT) using dynamic MRI in lung and tumour mobility during the breathing cycle. 20 patients with stage I non-small cell lung carcinoma were investigated using two dynamic gradient echo sequences with PAT (TrueFISP (fast imaging with steady precession), and fast low angle shot (FLASH). Craniocaudal distance from the apex to the diaphragm of the thorax and tumour mobility during the breathing cycle were measured. Signal-to-noise ratio (SNR) of the tumour was determined. In spite of the different temporal resolutions both trueFISP and FLASH sequence proved to be adequate to continuously measure lung motion and tumour mobility. SNR of the tumour was significantly higher using the trueFISP sequence than FLASH sequence (20.7+/-3.6 vs 5.8+/-2.3, p<0.01). Mobility of the tumour bearing hemithorax was significantly lower compared with the non-tumour bearing hemithorax (p<0.05). Dynamic MRI using PAT allows for continuous quantitative documentation of tumour mobility and lung motion. Because of the higher SNR, trueFISP sequence provides a better delineation of intrapulmonary lesions with a sufficient temporal resolution.
Extraventricular neurocytoma presenting with intratumoral hemorrhage.
2005-08-18

A case of extraventricular neurocytoma with spontaneous intratumoral hemorrhage is reported. A 47-year-old man presented with sudden left-sided hemiparesis. Magnetic resonance imaging revealed a right parietal subcortical mass with intratumoral hemorrhagic transformation and without contact to the ventricular system. After complete microsurgical removal, the tumor was histologically diagnosed as neurocytoma. Usually, the term "central neurocytoma" is restricted to neurocytic neoplasms arising within the cerebral ventricles. In the majority of the cases, these slow-growing, generally circumscribed lesions become symptomatic by obstructive hydrocephalus. Hemorrhagic onset is sporadically reported in the literature. In contrast to central neurocytomas, neurocytic lesions located within the brain parenchyma, so-called "extraventricular neurocytomas" are very uncommon. To the knowledge of the authors, this is the first case of an extraventricular neurocytoma with histological classic features presenting with intratumoral hemorrhage in adults.
X-RED: a satellite mission concept to detect early universe gamma ray bursts
2005-08-18

Gamma ray bursts (GRBs) are the most energetic eruptions known in the\\nUniverse. Instruments such as Compton-GRO/BATSE and the GRB monitor on BeppoSAX\\nhave detected more than 2700 GRBs and, although observational confirmation is\\nstill required, it is now generally accepted that many of these bursts are\\nassociated with the collapse of rapidly spinning massive stars to form black\\nholes. Consequently, since first generation stars are expected to be very\\nmassive, GRBs are likely to have occurred in significant numbers at early\\nepochs. X-red is a space mission concept designed to detect these extremely\\nhigh redshifted GRBs, in order to probe the nature of the first generation of\\nstars and hence the time of reionisation of the early Universe. We demonstrate\\nthat the gamma and x-ray luminosities of typical GRBs render them detectable up\\nto extremely high redshifts (z~10-30), but that current missions such as HETE2\\nand SWIFT operate outside the observational range for detection of high\\nredshift GRB afterglows. Therefore, to redress this, we present a complete\\nmission design from the science case to the mission architecture and payload,\\nthe latter comprising three instruments, namely wide field x-ray cameras to\\ndetect high redshift gamma-rays, an x-ray focussing telescope to determine\\naccurate coordinates and extract spectra, and an infrared spectrograph to\\nobserve the high redshift optical afterglow. The mission is expected to detect\\nand identify for the first time GRBs with z &gt; 10, thereby providing constraints\\non properties of the first generation of stars and the history of the early\\nUniverse.\\n