1304 NOVEL BOURGEONAL FRAGRANCE CONJUGATES FOR THE DETECTION OF PROSTATE CANCER
2012-04-01

You have accessJournal of UrologyProstate Cancer: Basic Research II1 Apr 20121304 NOVEL BOURGEONAL FRAGRANCE CONJUGATES FOR THE DETECTION OF PROSTATE CANCER Stefan Heckl, Alexander Sturzu, Sumbla Sheik, Uwe Klose, Hartmut Echner, Thomas Kalbacher, Martin Deeg, Marius Horger, Joerg Hennenlotter, Thomas Naegele, Tilman Todenhöfer, Arnulf Stenzl, Ulrike Ernemann, and Christian Schwentner Stefan HecklStefan Heckl Tuebingen, Germany More articles by this author , Alexander SturzuAlexander Sturzu Tuebingen, Germany More articles by this author , Sumbla SheikSumbla Sheik Tuebingen, Germany More articles by this author , Uwe KloseUwe Klose Tuebingen, Germany More articles by this author , Hartmut EchnerHartmut Echner Tuebingen, Germany More articles by this author , Thomas KalbacherThomas Kalbacher Tuebingen, Germany More articles by this author , Martin DeegMartin Deeg Tuebingen, Germany More articles by this author , Marius HorgerMarius Horger Tuebingen, Germany More articles by this author , Joerg HennenlotterJoerg Hennenlotter Tuebingen, Germany More articles by this author , Thomas NaegeleThomas Naegele Tuebingen, Germany More articles by this author , Tilman TodenhöferTilman Todenhöfer Tuebingen, Germany More articles by this author , Arnulf StenzlArnulf Stenzl Tuebingen, Germany More articles by this author , Ulrike ErnemannUlrike Ernemann Tuebingen, Germany More articles by this author , and Christian SchwentnerChristian Schwentner Tuebingen, Germany More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1651AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The current methods for detection of prostate cancer and prostate cancer lymph node metastases are unsatisfactory. Currently, no means of identifying metastasized lymph nodes other than biopsies is available. A prerequisite for potential imaging methods are markers which are exclusively found on prostate cancer cells. To complement the established markers like e.g. PCA3 – a noncoding mRNA sequence – and PSA – a serine protease – we investigated the ectopically expressed G-protein coupled olfactory receptor OR1D2 as a possible target for prostate-specific detection with its agonist bourgeonal which has been conjugated to two different fluorescent dyes. METHODS We performed mRNA expression analysis of the OR1D2 receptor mRNA by reverse transcriptase polymerase chain reaction on LNCaP prostate carcinoma cells and three other non-prostate derived carcinoma cell lines. Additionally, we used confocal laser scanning microscopy of in vitro cell culture and in vivo tumor xenografts on mice as well as flow cytometry to investigate uptake of fluorescent-dye-bound OR1D2-ligand bourgeonal into LNCaP prostate carcinoma, U373 glioma, U2OS osteosarcoma and Colo205 colon carcinoma cells. RESULTS We could confirm OR1D2 receptor mRNA overexpression as well as stronger uptake of both bourgeonal conjugates in vitro and in vivo for LNCaP cells compared to the non-prostate derived cell lines. Cytoplasmic accumulation and no adverse effects after in vitro and in vivo application of the conjugates were observed. CONCLUSIONS The conjugates represent a platform for the development of future prostate-specific imaging applications, e.g. detection of metastasized lymph nodes during surgery by intraoperative laser examination. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e529 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Stefan Heckl Tuebingen, Germany More articles by this author Alexander Sturzu Tuebingen, Germany More articles by this author Sumbla Sheik Tuebingen, Germany More articles by this author Uwe Klose Tuebingen, Germany More articles by this author Hartmut Echner Tuebingen, Germany More articles by this author Thomas Kalbacher Tuebingen, Germany More articles by this author Martin Deeg Tuebingen, Germany More articles by this author Marius Horger Tuebingen, Germany More articles by this author Joerg Hennenlotter Tuebingen, Germany More articles by this author Thomas Naegele Tuebingen, Germany More articles by this author Tilman Todenhöfer Tuebingen, Germany More articles by this author Arnulf Stenzl Tuebingen, Germany More articles by this author Ulrike Ernemann Tuebingen, Germany More articles by this author Christian Schwentner Tuebingen, Germany More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...