MP7-08 ESTABLISHMENT OF IN VIVO SMALL ANIMAL MODELS TO DETERMINE CELL MOBILITY AND VIABILITY AND IMAGING OF INJECTED MESENCHYMAL STEM CELLS IN THE PIG SPHINCTER
2014-03-28

You have accessJournal of UrologyImaging/Radiology II1 Apr 2014MP7-08 ESTABLISHMENT OF IN VIVO SMALL ANIMAL MODELS TO DETERMINE CELL MOBILITY AND VIABILITY AND IMAGING OF INJECTED MESENCHYMAL STEM CELLS IN THE PIG SPHINCTER Karl-Dietrich Sievert, Susanne Will, Julia G. Mannheim, Kerstin Fuchs, Jörg Schmehl, Gerd Grözinger, Rüdiger Bantleon, Martin Vaegler, Petros Martirosian, Fritz Schick, Claus D. Claussen, Bernd J. Pichler, and Ulrich Kramer Karl-Dietrich SievertKarl-Dietrich Sievert More articles by this author , Susanne WillSusanne Will More articles by this author , Julia G. MannheimJulia G. Mannheim More articles by this author , Kerstin FuchsKerstin Fuchs More articles by this author , Jörg SchmehlJörg Schmehl More articles by this author , Gerd GrözingerGerd Grözinger More articles by this author , Rüdiger BantleonRüdiger Bantleon More articles by this author , Martin VaeglerMartin Vaegler More articles by this author , Petros MartirosianPetros Martirosian More articles by this author , Fritz SchickFritz Schick More articles by this author , Claus D. ClaussenClaus D. Claussen More articles by this author , Bernd J. PichlerBernd J. Pichler More articles by this author , and Ulrich KramerUlrich Kramer More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.331AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To strengthen the physiological function of the urethral sphincter, different aspects of cell-based treatments were investigated. The aim was to develop and provide preclinical and clinical-translational imaging platforms (Positron Emission Tomography, Optical Imaging and Magnetic Resonance Imaging (MRI)). For cell detection iron (Resovist), fluorescent (Cy5) and radioactive ([64Cu]PTSM) markers were applied to establish methods that enable visualization of therapeutic mesenchymal stem cells (MSC) in vitro and in vivo using rat and minipig animal models. Examinations of the porcine sphincter were applied with high resolution MRI, after iron cell labeling. METHODS In vitro titrations of different cell amounts were performed: 0.031 – 1.0 mio adipose tissue-derived stem cells (ADSC) from two patients were labeled with Cy5. The viability of the labeled cells was evaluated for ten days to determine the labeling influence on the cells. Complementary experiments with [64Cu] PTSM were also performed over two days. For the examination of the female minipig animal model, a 3T MR scanner was used. The anatomy of urethra and sphincter was acquired using a high resolution T2-weighted turbo spin echo sequence. A gradient echo sequence with multiple echo times was utilized to identify and verify locations of iron particles, based on visual inspection. The distribution of the injected cells was then correlated with histological findings. RESULTS Measurements of the minipig sphincter muscle, three weeks and three months after MSC injection, showed clearly visible iron deposits inside the sphincter muscle. Additionally, iron depositions could be verified even six months after stem cell injection. The comparison of MRI and tissue sections showed preeminent correlation of size and location of iron labeled cells. The optical signal decreased over time due to ongoing proliferation of cells. However, cell viability with [64Cu] PTSM or Cy5 due to the labeling was not yet determined. Application of 0.5 – 1.0 mio cells was found to be ideal for future experiments. CONCLUSIONS Further Cy5 and [64Cu] PTSM experiments, using MSC from bone marrow and placenta, are planned to investigate the efficiency of different stem cell types. Against initial expectations iron deposits were detectable six months after injection in the minipig animal model, so further MRI measurements should be applied. A method for quantitative analysis of iron deposition could be useful for the monitoring of cell proliferation and will be developed in the future. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e68 Advertisement Copyright & Permissions© 2014Metrics Author Information Karl-Dietrich Sievert More articles by this author Susanne Will More articles by this author Julia G. Mannheim More articles by this author Kerstin Fuchs More articles by this author Jörg Schmehl More articles by this author Gerd Grözinger More articles by this author Rüdiger Bantleon More articles by this author Martin Vaegler More articles by this author Petros Martirosian More articles by this author Fritz Schick More articles by this author Claus D. Claussen More articles by this author Bernd J. Pichler More articles by this author Ulrich Kramer More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...