MP1-16 DETECTION OF APOPTOTIC AND NON-APOPTOTIC DISSEMINATED TUMOR CELLS IN THE BONE MARROW AT THE TIME OF RADICAL PROSTATECTOMY
2015-03-31

You have accessJournal of UrologyProstate Cancer: Markers I1 Apr 2015MP1-16 DETECTION OF APOPTOTIC AND NON-APOPTOTIC DISSEMINATED TUMOR CELLS IN THE BONE MARROW AT THE TIME OF RADICAL PROSTATECTOMY Tilman Todenhöfer, Jörg Hennenlotter, Frank Faber, Ursula Kühs, Tanja Fehm, Diethelm Wallwiener, David Schilling, Georgios Gakis, Simone Bier, Johannes Mischinger, Stefan Aufderklamm, Arnulf Stenzl, and Christian Schwentner Tilman TodenhöferTilman Todenhöfer More articles by this author , Jörg HennenlotterJörg Hennenlotter More articles by this author , Frank FaberFrank Faber More articles by this author , Ursula KühsUrsula Kühs More articles by this author , Tanja FehmTanja Fehm More articles by this author , Diethelm WallwienerDiethelm Wallwiener More articles by this author , David SchillingDavid Schilling More articles by this author , Georgios GakisGeorgios Gakis More articles by this author , Simone BierSimone Bier More articles by this author , Johannes MischingerJohannes Mischinger More articles by this author , Stefan AufderklammStefan Aufderklamm More articles by this author , Arnulf StenzlArnulf Stenzl More articles by this author , and Christian SchwentnerChristian Schwentner More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.179AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES In a high proportion of patients with clinically localized malignancies, disseminated tumor cells (DTC) can be detected in the bone marrow (BM). Their role in prostate cancer (PC) is discussed controversially and their prognostic value is not clear. The aim of the present study was to assess the prognostic value of apoptotic and non apoptotic DTCs in the BM of PC patients with a high risk of disease recurrence. METHODS 248 patients with clinically localized PC undergoing radical prostatectomy with features of increased risk of recurrence (PSA ≥10 ng/ml or Gleason score ≥7b or pT ≥3) underwent intraoperative BM aspiration biopsy of the iliac crest. Presence of DTCs was evaluated by immunocytochemistry for cytokeratines and the apoptosis marker caspase-cleaved cytokeratin 18 (M30). Results of immunocytochemistry were correlated with clinical and pathological parameters and clinical outcome of the patients. RESULTS In 47 of 248 (19.0%) patients, DTCs were detected at time of radical prostatectomy. Expression of the apoptosis marker M30 was present in DTCs of 17 of these 47 patients (36.2%). No correlation between the presence of DTCs and tumor stage, nodal stage, prostate-specific antigen, or Gleason score was observed. After a median-follow-up of 58 months (23–76), no differences in biochemical recurrence, development of metastases and cancer-specific death were observed between patients with and without DTCs. Expression of M30 by DTCs did not correlate with histopathologic parameters and outcome. CONCLUSIONS For the first time in patients with PC, DTCs in BM were evaluated for immunocytological features indicating apoptosis. The presence of apoptotic or non-apoptotic DTCs at the time of prostatectomy does not influence clinical outcome in this single-centre cohort of PC patients with increased risk for disease recurrence. Due to conflicting data on their prognostic value, the determination of DTCs in BM aspirates of patients with PC cannot be recommended as standard procedure. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e7 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tilman Todenhöfer More articles by this author Jörg Hennenlotter More articles by this author Frank Faber More articles by this author Ursula Kühs More articles by this author Tanja Fehm More articles by this author Diethelm Wallwiener More articles by this author David Schilling More articles by this author Georgios Gakis More articles by this author Simone Bier More articles by this author Johannes Mischinger More articles by this author Stefan Aufderklamm More articles by this author Arnulf Stenzl More articles by this author Christian Schwentner More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...