julilili42/papers huggingface dataset:
Exercise Intensity and Duration Affect Blood Soluble HSP72
2005-09-01

Soluble heat shock protein 72 (sHSP72) is suggested to play a role as a signalling molecule in the immune response to exercise. We were interested in whether duration and intensity of endurance running affect the level of inducible sHSP72 in the plasma/serum of endurance athletes. In the first part of the study, the influence of a continuous treadmill run of 60 min (CR) with an intensity of 75 % VO2max, a long treadmill run of 120 min (LR) with an intensity of 60 % VO2max, an extensive interval training program (IT; 10 x 1000 m, ca. 35 min, VO2max 88 %), and a competitive marathon run (MA) within 260 +/- 39 min (VO2max ca. 65 %) on the release of sHSP72 into the peripheral blood was tested. Blood samples were drawn before and directly after exercise, as well as 0.5, 1, 3, 24 h after exercise to determine sHSP72 levels. Secondly, we compared the effects of two exercise bouts with identical duration (23.7 +/- 7 min) but different intensities (Exhaustive exercise (ET) at 80 % VO2max vs. moderate exercise (MT) at 60 % VO2max) on sHSP72 concentration. The sHSP72 levels in plasma/serum were analyzed using an enzyme immunoassay specific for inducible HSP72 (Stressgen,Victoria, Canada). Early, significant increases of sHSP72 were detected immediately after all types of exercise with highest levels after MA. ET induced significantly higher levels of sHSP72 compared with MT. Long-lasting, competitive endurance exercise induced a more pronounced response of sHSP compared with more intensive but shorter exercise. Exercise intensity was also an important influencing factor. A duration- and intensity-dependent role for sHSP72 in the exercise-induced changes of the immune response may be assumed.
Effects of Imatinib on Normal Hematopoiesis and Immune Activation
2005-09-01

The selective tyrosine kinase inhibitor imatinib (Glivec; Novartis International, Basel, Switzerland, http://www.glivec.com/content/home.jsp) is increasingly used for the treatment of Philadelphia chromosome-positive leukemias and other malignancies. In principle, the drug is well tolerated and clinical side effects are mostly moderate. However, it was shown that imatinib can affect the function of normal, nonmalignant cells, resulting in myelosuppression in treated patients. Recently, it has been demonstrated that imatinib might affect mobilization, proliferation, and differentiation of hematopoietic progenitor cells while leaving hematopoietic stem cells unaffected. Furthermore, in several in vitro studies and animal models, it was demonstrated that imatinib can affect the function and differentiation of antigen-presenting cells and inhibit the effector functions of T lymphocytes. Moreover, the induction of specific cytotoxic T cells seems to be impaired in chronic myeloid leukemia (CML) patients treated with imatinib compared with patients receiving interferon-alpha. This is of importance because some of the therapeutic effects in the treatment of patients with CML are mediated by the induction of leukemia-specific T-cell responses. Further studies investigating the effects of imatinib on normal hematopoiesis are of interest as they might lead to a better understanding of the clinically observed side effects and also might help identify new therapeutic applications of the drug, possibly in Bcr-Abl-negative myeloproliferative disorders and potentially as an immunomodulatory agent.
Effects of MPEP on expression of food‐, MDMA‐ or amphetamine‐conditioned place preference in rats
2005-09-01

Abstract Recent studies have revealed the effectiveness of 2‐methyl‐6‐(phenylethynyl)pyridine (MPEP), a highly selective antagonist of metabotropic glutamate receptors subtype 5 (mGluR5), in conditioned drug reward. In a previous study we showed that MPEP blocks expression of context‐conditioned morphine‐ but not cocaine reward in the rat. The present study now examines the effectiveness of MPEP in the expression of context‐conditioned food, MDMA (‘ecstasy?) or amphetamine reward. Therefore, three groups of rats were conditioned either to food, MDMA or amphetamine in the conditioned place preference (CPP) paradigm. After conditioning, CPP expression and locomotion were determined simultaneously in the presence and absence of the respective reward (i.e. food or drug), or after application of 50?mg/kg MPEP (the dose that was most effective in reducing morphine CPP expression in our previous study). As a result, MPEP reduced locomotion in all groups. Furthermore, only expression of amphetamine CPP was inhibited by MPEP, while expression of food and MDMA CPP was not affected, suggesting that the MPEP‐induced inhibition of amphetamine CPP expression was not causally linked to the reduction of locomotion. Overall, we conclude that MPEP reduces expression of context‐conditioned amphetamine but not MDMA or food reward.
Modeling the influence of epikarst evolution on karst aquifer genesis: A time‐variant recharge boundary condition for joint karst‐epikarst development
2005-09-01

The epikarst, a zone of increased weathering near the land surface, determines the distribution of recharge to a karst aquifer in both space and time. It links climatic and near‐surface geological conditions with the karstification of a limestone aquifer, defining both the hydraulic and the chemical boundary conditions for the development of the karst system. Realistic modeling of the epikarst is therefore a prerequisite for the simulation of karst aquifer genesis. A conceptual model of the joint karst‐epikarst evolution is presented in this paper. An epikarst module is developed and implemented in a numerical continuum‐discrete conduit flow model for karst genesis, which accounts for the joint evolution of the epikarst and the main karstic conduit network under unconfined conditions. The influence of epikarst genesis on the evolution of the underlying karst aquifer is investigated in four scenarios. It is found that only the interaction of epikarst and initial heterogeneity in the underlying carbonate rock leads to the development of a dendritic cave system. If no heterogeneity in the initial conduit network or in the recharge distribution is included, maze‐type caves develop.
Perceived Dynamics of Static Images Enables Emotional Attribution
2005-09-01

Perception of intentions and dispositions of others is an essential ingredient of adaptive daily-life social behaviour. Dynamics of moving images leads to veridical perception of social attributes. Anecdotal observations in art, science, and popular culture indicate that dynamic imbalance can be revealed in static images. Here, we ask whether perceived dynamics of abstract figures is related to emotional attribution. Participants first estimated instability of geometric shapes rotated in 15° steps in the image plane, and then rated the intensity of basic emotions that can be ascribed to the figures. We found no substantial link between the deviation of the figures from the vertical orientation and perceived instability. Irrespective of shape, a strong positive correlation was found between negative emotions and perceived instability. By contrast, positive emotions were inversely linked with deviation of the figure from vertical orientation. The work demonstrates for the first time that dynamics conveyed by static images enables specific emotional attributions, and agrees well with the assumption that neural networks for production of movements and understanding the dispositions of others are intimately linked. The findings are also of importance for exploring the ability to reveal social properties through dynamics in normal and abnormal development, for example in patients with early brain injury or autistic spectrum disorders.
Rapid Identification of Staphylococcus aureus in Blood Cultures by a Combination of Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes and Flow Cytometry
2005-09-01

ABSTRACT Fluorescence in situ hybridization (FISH) using peptide nucleic acid probes (PNAs) allows the identification of Staphylococcus aureus from human blood culture samples. We present data revealing that the combination of PNA FISH and flow cytometry is a possible approach for the noncultural identification of staphylococci in blood cultures.
Human Parvovirus B19: A New Emerging Pathogen of Inflammatory Cardiomyopathy
2005-09-01

Summary The human parvovirus B19 (PVB19), an erythrovirus causing diverse clinical manifestations ranging from asymptomatic or mild to more severe outcomes such as hydrops fetalis, is the only known human pathogenic parvovirus so far. Although enteroviruses have long been considered the most common cause of inflammatory cardiomyopathy, PVB19 is emerging as a important candidate. Recent studies have indicated an association of PVB19 with paediatric and adult inflammatory cardiac disease. However, whether or not PVB19 has an impact on inflammatory cardiomyopathy in adult patients is still unclear. The first hints for a possible aetiopathogenetic role of the PVB19‐infection and the development of cardiac dysfunction were demonstrated by molecular biology utilizing in situ hybridization (ISH) and polymerase chain reaction (PCR). According to available evidence, PVB19‐associated inflammatory cardiomyopathy is characterized by infection of endothelial cells of small intracardiac arterioles and venules, which may be associated with endothelial dysfunction, impairment of myocardial microcirculation, and penetration of inflammatory cells into the myocardium.
Cell microarrays
2005-09-01

In the postgenomic era, DNA and protein arrays are increasing the speed at which knowledge is gathered on gene expression in cells and tissues. At the same time, researchers realize that a miniaturized and parallelized analysis of whole cells may equally expedite the acquisition of data describing cellular properties and function. Researchers are starting to explore means of generating and using cell microarrays to investigate cells at higher throughput. In this initial phase of exploration, cell microarrays are being developed for various cellular analyses including the effects of gene expression, cellular reactions to the biomolecular environment, and profiling of cell surface molecules. This article will provide an overview of different types of eukaryotic cell microarrays described to date, how they are generated, and their fields of application.
New Unsymmetrical Zinc‐Phthalocyanine Conjugated with One Azo‐Dye Moiety: Synthesis via Opening the Fused Triazole Ring and Spectral Properties
2005-09-01

Abstract A new method for the preparation of compounds containing an azo‐dye moiety via opening the activated triazole ring upon coupling with nucleophiles was successfully applied to a mono‐triazole‐fused phthalocyaninato zinc complex 1 . The prepared unsymmetrical zinc‐phthalocyanine 3 conjugated with a 2‐hydroxy‐1‐naphthylazo moiety in the periphery was characterized by means of UV/Vis‐, FTIR‐, 1 H and 13 C NMR spectroscopy, MALDI‐TOF spectrometry, and elemental analysis, and the data support its structure. The assignment of signals in the 1 H and 13 C NMR spectra of compound 3 was based on the data from 2‐D CH‐COSY and HC‐HMBC measurements (C–H coupling across one bond and long‐range H–C coupling). The compound exhibits interesting spectroscopic properties, indicating high acidity of the hydroxy group. This phthalocyanine behaves as a strongly solvatochromic compound and can exist in different forms depending on the concentration and nature of solvent. Additionally, photodecomposition of 3 in chloroform and tetrahydrofuran was found to proceed via different pathways. (© Wiley‐VCH Verlag GmbH &amp; Co. KGaA, 69451 Weinheim, Germany, 2005)
Dibaryon model for nuclear force and the properties of the 3N system
2005-09-01

The dibaryon model for interaction, which implies the formation of an\\nintermediate six-quark bag dressed by a -field, is applied to the \\nsystem, where it results in a new three-body force of scalar nature between the\\nsix-quark bag and a third nucleon. A new multicomponent formalism is developed\\nto describe three-body systems with nonstatic pairwise interactions and\\nnon-nucleonic degrees of freedom. Precise variational calculations of \\nbound states are carried out in the dressed-bag model including the new scalar\\nthree-body force. The unified coupling constants and form factors for and\\n force operators are used in the present approach, in a sharp contrast to\\nconventional meson-exchange models. It is shown that this three-body force\\ngives at least half the total binding energy, while the weight of\\nnon-nucleonic components in the H and He wavefunctions can exceed 10%.\\nThe new force model provides a very good description of bound states with\\na reasonable magnitude of the coupling constant. A new Coulomb \\nforce between the third nucleon and dibaryon is found to be very important for\\na correct description of the Coulomb energy and r.m.s. charge radius in He.\\nIn view of the new results for Coulomb displacement energy obtained here for\\nA=3 nuclei, an explanation for the long-term Nolen--Schiffer paradox in nuclear\\nphysics is suggested. The role of the charge-symmetry-breaking effects in the\\nnuclear force is discussed.\\n
A comparison of the aging and apoptotic transcriptome of
2005-09-01

In this paper, we present the results of global transcript analysis by the microarray technique of senescent and apoptotic yeast cells. We compared young daughter and old mother cells isolated by elutriation centrifugation, and non-apoptotic and apoptotic cells induced either by a temperature shift of the cdc48(S565G) temperature-sensitive mutant or of the orc2-1 temperature-sensitive mutant. The majority of all genes found to be differentially regulated in these three physiological situations was upregulated, indicating that a cellular death process was initiated rather than an unspecific shut-down of gene expression due to immediate killing. The functional classes of genes upregulated in all three conditions were largely the same, although individual genes were in many cases not identical. The largest group of genes involved were nuclear genes coding for mitochondrial components or functions, which is understandable given the fact that apoptosis can be triggered by mitochondrially generated oxygen radicals and that mitochondria play an important role in the execution of apoptosis. Other functional classes consisted of genes involved in DNA damage response, in cell cycle regulation and checkpoints, in DNA repair, and in membrane lipid and cell wall synthesis. These functional classes represent the response of the cell to the known cellular insults, which occur during aging and apoptosis. As we have shown previously, final-stage senescent yeast mother cells (of the wild-type) are apoptotic.
MAGED4 – expression in renal cell carcinoma and identification of an HLA-A*25-restricted MHC class I ligand from solid tumor tissue
2005-09-01

MAGE derived HLA ligands have repeatedly been shown to elicit T-cell responses against tumor cells. In renal cell carcinoma (RCC), however, only few T-cell epitopes from cancer testis antigens have been described. To identify potential candidates, we applied a combined approach of microarray/qPCR expression analysis and sequencing of HLA ligands from RCC by mass spectrometry. We analyzed the expression of 21 MAGE genes in ten RCC samples and two glioblastoma samples and could identify the first MHC class I ligand NIGDEALIGRW from MAGED4 presented by HLA-A*25 on RCC solid tumor tissue. MAGED4 was expressed in 30% of RCC and both glioblastoma samples. Among the other MAGE family members only MAGEB2 and -C1 and the broadly expressed MAGED1, -D2, -F1 and -H1 were expressed in RCC. Ligands from MAGED4 could thus be interesting tumor-associated antigens in a subset of RCC, even though the identified ligand is presented by a rather rare allele.
Laparoscopic Retroperitoneal Lymph-Node Dissection with the Waterjet Is Technically Feasible and Safe in Testis-Cancer Patient
2005-09-01

The waterjet allows the safe and complete removal of lymphatic tissue, leaving vulnerable anatomic structures intact. It can decrease the learning curve of laparoscopic RPLND and contribute to better acceptance of this procedure.
Convergence results for compound Poisson distributions and applications to the standard Luria–Delbrück distribution
2005-09-01

We provide a scaling for compound Poisson distributions that leads (under certain conditions on the Fourier transform) to a weak convergence result as the parameter of the distribution tends to infinity. We show that the limiting probability measure belongs to the class of stable Cauchy laws with Fourier transform t ↦ exp(− c | t |− i at log| t |). We apply this convergence result to the standard discrete Luria–Delbrück distribution and derive an integral representation for the corresponding limiting density, as an alternative to that found in a closely related paper of Kepler and Oprea. Moreover, we verify local convergence and we derive an integral representation for the distribution function of the limiting continuous Luria–Delbrück distribution.
CYSTICERCOSIS IN CHRONIC PSYCHIATRIC INPATIENTS FROM A VENEZUELAN COMMUNITY
2005-09-01

Cysticercosis due to Taenia solium infection is endemic in developing countries of the Americas, Asia, and Africa. This study was designed to establish the prevalence of cysticercosis in 158 inpatients of a psychiatric institution in the state of Tachira (Venezuela) and in 127 healthy control subjects. Positive blood tests for cysticercosis by Western blotting were recorded in 18.35% of the patients and in 1.57% of the controls. Individuals with mental retardation were found to carry an increased risk of cysticercosis (RR: 2.92; 1.22 &lt; 2.92 &gt; 7.0; P &lt; 0.05) compared with patients with other psychiatric disorders. Taeniasis by Taenia spp. was not demonstrated in the patient group, although a high incidence of infection by other helminths (95.1%) was detected. The high prevalence of cysticercosis in the psychiatric inpatient group, compared with healthy individuals, and the lack of a differential diagnosis of neurocysticercosis suggest cerebral cysticercosis in a large proportion of these patients. Cysticercosis could be the origin of the psychiatric disorders of these patients and may also be due to contact with the parasite in an environment with poor hygiene conditions and a deficient health care system.
A large spin-up rate measured with INTEGRAL in the high mass X-ray binary pulsar SAX J2103.5+4545
2005-09-01

\\n The High Mass X-ray Binary Pulsar SAX J2103.5+4545 has been observed with INTEGRAL several times during the last outburst in 2002–2004. We report a comprehensive study of all INTEGRAL observations, allowing a study of the pulse period evolution during the recent outburst. \\nWe measured a very rapid spin-up episode, lasting ~130 days, which decreased the pulse period\\nby ~1.8 s. The spin-up rate, ~ -1.5 10-7 s s-1, \\nis the largest ever measured for SAX J2103.5+4545, and it is among the\\nfastest for an accreting pulsar. The pulse profile shows evidence for\\ntemporal variability, apparently not related to the source flux \\nor to the orbital phase. The X-ray spectrum is hard and there is significant emission up to\\n150 keV. A new derivation of the orbital period, based on RXTE data, is also reported.\\n\\n\\n
Derivate der 1,3-Dimethyl-5-methylen-barbitursüure / Derivatives of 1,3-Dimethyl-5-methylenebarbituric Acid
2005-09-01

1,3-Dimethylbarbituric acid (1, R = Me) reacts with aqueous formaldehyde in the presence of pyridine to give 1,3-dimethyl-2,4,6-trioxo-5-pyridinomethyl-1,3-perhydrodiazin-5-ylpyridinium ylide (6) in good yield. From 6 and 2,3-dihydro-1,3-diisopropyl-4,5-dimethylimidazol- 2-ylidene (7), the zwitterionic imidazolium derivative 1,3-dimethyl-2,4,6-trioxo-5-(1,3-diisopropyl- 4,5-dimethylimidazoliomethyl)-1,3-perhydrodiazin-5-yl-imidazolium ylide (8) is obtained. Similarly, the phosphonium compound 1,3-dimethyl-2,4,6-trioxo-5-triphenylphosphoniomethyl-1,3- perhydrodiazin-5-y l-phosphonium ylide (9) is obtained from 6 and PPh 3 . The crystal structures of 8 and 9 are reported.
Intraconal Grease-Gun Injury: A Therapeutic Dilemma
2005-09-01

The case of a 31-year-old man with an accidental grease-gun injury to his left orbital region is presented. CT and MRI showed a well-delineated intraconal cyst in the superior aspect of the left orbit surrounded by a significant inflammatory response. The patient was followed for 11 months. Diplopia was not reported at any time, and visual acuity, visual fields, and all other examinations remained normal. Uneventful surgical removal was performed at the patient's request. Histopathologic examination demonstrated a typical picture of lipogranulomatous inflammation. In a review of the accessible literature, only four cases of intraorbital grease-gun injuries were detected; all of them submitted to early surgical removal. We conclude, however, that an intraconal oil/grease cyst can be carefully monitored and--in the absence of symptoms--must not necessarily be removed.
ASSESSMENT OF A SIMPLIFIED METHOD FOR COUNTING LEUKOCYTIC MALARIA PIGMENT
2005-09-01

Severe and fatal malaria is associated with the increased presence of malaria hemozoin in peripheral phagocytes. Large studies of this relationship are hampered by the fact that identifying and counting phagocytes on thick blood smears is time consuming. Distinguishing which mononuclear cells are monocytes and which granulocytes are neutrophils requires time and careful training. In this study, we evaluated a simplified method in which only the proportions of hemozoin-containing mononuclear cells and granulocytes are counted. Thick blood films from 471 Gabonese children with malaria were evaluated. We found a linear relationship and a strong correlation between the proportions of hemozoin-containing monocytes versus mononuclear cells (r = 0.85) and neutrophils versus polymorphonuclear cells (r = 0.93), respectively. The two methods had similar predictive values, as estimated by receiver operating characteristics curves. This simplified method can be used to estimate the amount of extra-erythrocytic pigment in peripheral blood, and we suggest that it may be particularly suitable for very large studies.
A NEW FOSSIL HOVERFLY (INSECTA, DIPTERA: SYRPHIDAE) FROM THE RANDECK MAAR (EARLY MIOCENE, SOUTH-WEST GERMANY)
2005-09-01

Abstract: Helophilus nothobombus sp. nov. (Diptera: Syrphidae) is described from Early Miocene lake sediments of the Randeck Maar (Swabian Alb, south-west Germany) and compared to other fossil species of the genus. Helophilus primarius Germar, 1837 and Helophilus villeneuvi Théobald, 1937 do not show diagnostic features that allow assignment to the genus Helophilus or even to the tribe Eristalini. Helophilus miocaenicus (Stackelberg, 1925) and H. nothobombus thus are the only plausible fossil species of the genus. The rat-tailed maggots of Helophilus most probably were part of the biocoenosis of the shores of the Randeck Maar lake. The frequency of larvae of Stratiomyidae shows that there were also suitable habitats for species of Helophilus and related genera.