1305 THE GASTRIN/CHOLECYSTOKININ-B RECEPTOR ON PROSTATE CELLS – A NOVEL TARGET FOR BIFUNCTIONAL PROSTATE CANCER IMAGING
2012-04-01

You have accessJournal of UrologyProstate Cancer: Basic Research II1 Apr 20121305 THE GASTRIN/CHOLECYSTOKININ-B RECEPTOR ON PROSTATE CELLS – A NOVEL TARGET FOR BIFUNCTIONAL PROSTATE CANCER IMAGING Stefan Heckl, Alexander Sturzu, Sumbla Sheik, Uwe Klose, Hartmut Echner, Thomas Kalbacher, Martin Deeg, Marius Horger, Joerg Hennenlotter, Thomas Naegele, Tilman Todenhöfer, Arnulf Stenzl, Ulrike Ernemann, and Christian Schwentner Stefan HecklStefan Heckl Tuebingen, Germany More articles by this author , Alexander SturzuAlexander Sturzu Tuebingen, Germany More articles by this author , Sumbla SheikSumbla Sheik Tuebingen, Germany More articles by this author , Uwe KloseUwe Klose Tuebingen, Germany More articles by this author , Hartmut EchnerHartmut Echner Tuebingen, Germany More articles by this author , Thomas KalbacherThomas Kalbacher Tuebingen, Germany More articles by this author , Martin DeegMartin Deeg Tuebingen, Germany More articles by this author , Marius HorgerMarius Horger Tuebingen, Germany More articles by this author , Joerg HennenlotterJoerg Hennenlotter Tuebingen, Germany More articles by this author , Thomas NaegeleThomas Naegele Tuebingen, Germany More articles by this author , Tilman TodenhöferTilman Todenhöfer Tuebingen, Germany More articles by this author , Arnulf StenzlArnulf Stenzl Tuebingen, Germany More articles by this author , Ulrike ErnemannUlrike Ernemann Tuebingen, Germany More articles by this author , and Christian SchwentnerChristian Schwentner Tuebingen, Germany More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1652AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The means of identifying prostate carcinoma and its metastases are limited. The contrast agents used in magnetic resonance imaging clinical diagnostics are not taken up into the tumor cells, but only accumulate in the interstitial space of the highly vascularized tumor. METHODS We examined the gastrin/cholecystokinin-B receptor as a possible target for prostate-specific detection using the N-terminal seven amino acid sequence of the gastrin peptide hormone. The correct sequence and a scrambled control sequence were coupled to the fluorescent dye rhodamine and the magnetic resonance imaging contrast agent gadolinium (Gd)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). Expression analysis of the gastrin receptor mRNA was performed by reverse transcriptase polymerase chain reaction on PC3 prostate carcinoma cells, U373 glioma, U2OS osteosarcoma and Colo205 colon carcinoma cells. After having confirmed elevated expression of gastrin receptor in PC3 cells and very low expression of the receptor in Colo205 cells, these two cell lines were used to create tumor xenografts on nude mice for in vivo experiments. RESULTS Confocal lasers scanning microscopy and magnetic resonance imaging showed a high uptake of the correct conjugate into the PC3 xenografts. Uptake of the scrambled conjugate into the PC3 xenografts was much weaker while the Colo205 xenografts showed no marked uptake of any of the conjugates. In vitro experiments comparing the correct and scrambled conjugates on PC3 cells by magnetic resonance relaxometry and fluorescence-activated cell sorting confirmed markedly higher uptake of the correct conjugate. CONCLUSIONS The investigations show that the gastrin receptor is a promising tumor cell surface target for future prostate-cancer-specific imaging applications. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e529 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Stefan Heckl Tuebingen, Germany More articles by this author Alexander Sturzu Tuebingen, Germany More articles by this author Sumbla Sheik Tuebingen, Germany More articles by this author Uwe Klose Tuebingen, Germany More articles by this author Hartmut Echner Tuebingen, Germany More articles by this author Thomas Kalbacher Tuebingen, Germany More articles by this author Martin Deeg Tuebingen, Germany More articles by this author Marius Horger Tuebingen, Germany More articles by this author Joerg Hennenlotter Tuebingen, Germany More articles by this author Thomas Naegele Tuebingen, Germany More articles by this author Tilman Todenhöfer Tuebingen, Germany More articles by this author Arnulf Stenzl Tuebingen, Germany More articles by this author Ulrike Ernemann Tuebingen, Germany More articles by this author Christian Schwentner Tuebingen, Germany More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...