julilili42/papers huggingface dataset:
The Indonesian prefixes PE- and PEN-: A study in productivity and allomorphy
2019-02-07

This study examines two nominalizing prefixes in Indonesian: PE- and PEN-, which derive nouns from verbs with a range of meanings similar to that found in -er suffix in English. The prefix PE- is form-invariant, whereas PEN- has several nasal allomorphs. Given their similarity in form and function, the question arises of whether PE- and PEN- are allomorphs. We conducted a corpus-based analysis of their productivity, using the written Indonesian corpus in the Leipzig Corpora Collection. In this corpus, PEN- is apparently more productive than PE-. Interestingly, the frequency of words with PEN- correlates significantly with the productivity of the corresponding base verbs. In addition, PEN- is more integrated into the verbal system; verbs that have PEN- are part of larger verb families. PEN- attaches almost exclusively to verbs and creates nouns denoting agents and instruments. By contrast, PE- creates nouns denoting agents and patients and attaches not only to verbs but also to nouns and adjectives. For derived words with PE-, there is no significant correlation between the frequency of the nominalization and the frequency of its base. PE- also does not participate in the linearity of the productivity of the allomorphs of base and derived words that characterizes PEN-. Words with PE- are also more often input to further reduplication and inflectional variants than is the case for PEN-. This corpus-based research thus illustrates that affixes can have different qualitative and quantitative properties, although at first blush they look like allomorphs. Our analyses justify their treatment in the Indonesian literature as separate prefixes.
The Biology and Therapeutic Applications of Red Blood Cell Extracellular Vesicles
2019-02-07

This chapter focuses on the biology of red blood cell extracellular vesicles (RBCEVs) in normal and diseased conditions, and the potential application of RBCEVs in treatment. Extracellular vesicles (EVs) refer to membranous vesicles secreted by cells into the extracellular environment. EV biology belongs to a rapidly developing field in biomedical sciences. EVs represent a natural mode of cell-to-cell communication, which makes them suitable for delivery of therapeutic agents, such as nucleic acids and proteins, in the body. In particular, RBCEVs feature a wide range of benefits in drug delivery as compared to extracellular vesicles derived from other cell types. In comparison to other delivery systems currently available, RBCEVs are nontoxic, low immunogenic, conveniently obtainable, and easy to use and store. Therefore, RBCEVs boast promising and exceptional advantages in overcoming various limitations of conventional therapeutics.
A fronto-temporo-parietal network disambiguates potential objects of joint attention
2019-02-07

Abstract We use the other’s gaze direction to identify her/his object of interest and to shift our attention to the same object, i.e. to establish joint attention. However, gaze direction may not be sufficient to unambiguously identify the object of interest as the other’s gaze may hit more than one object. In this case, the observer must use a priori information to disambiguate the object choice. Using fMRI, we suggest that the disambiguation is based on a 3-component network. A first component, the well-known ‘gaze following patch’ in the posterior STS is activated by gaze following per se. BOLD activity here is determined exclusively by the usage of gaze direction and is independent of the need to disambiguate the relevant object. On the other hand, BOLD activity revealing a priori information for the disambiguation and starting early enough to this end is confined to a patch of cortex at the inferior frontal junction. Finally, BOLD activity reflecting the convergence of both, a priori information and gaze direction, needed to shift attention to a particular object location is confined to the posterior parietal cortex.
Effect of Ocriplasmin on objectively assessed retinal function after treatment of vitreomacular diseases
2019-02-07

Abstract Background The scope of the study was the functional assessment of possible effects of intravitreally injected ocriplasmin using electroretinographical ( ERG ) examinations. Methods Seven subjects suffered from pathologies within the label of ocriplasmin (Jetrea ® ) were examined in the study. An extended international society for clinical electrophysiology of vision ERG protocol was used for baseline recordings (before treatment) and was repeated for comparison 3 months after treatment with intravitreally injected ocriplasmin. All subjects were examined using 6 steps and a 9 Hz flicker protocol as scotopic flicker response under dark‐adapted conditions. Under light‐adapted conditions, two single‐flash responses (standard flash and On/Off protocol) and a 31 Hz flicker response was used for functional assessment. Results A significant reduction of amplitudes was found for the lowest stimulation intensity (0.0001 cd.s/m2) under dark‐adapted conditions after treatment (p = 0.027). All other parameters were not significantly different after treatment including the scotopic flicker response. Under light‐adapted state, the a‐ and b‐wave amplitudes were not significantly altered after treatment for single‐flash and flicker responses. The On‐ and Off‐responses were not significantly different between baseline and after the treatment. Conclusions One single significant difference of all examined parameters mirrors a difference due to chance, even if a negative effect of proteolytic enzymes on laminin and fibronectin of retinal cells cannot be entirely excluded. Nevertheless, this result should be respected in order to ensure a safe use of ocriplasmin for patients with vitreoretinal disorders.
Phosphine-Stabilized Digermavinylidene
2019-02-08

An intramolecular germylene-phosphine Lewis pair (1) was reacted with germanium dichloride to give in 92% yield a phosphine adduct of a chloro substituted germyl-germylene (2). After reduction of this dichloride with strong reductants like the Mg(I) reagent {(<sup>Mes</sup>Nacnac)Mg}<sub>2</sub> (72% yield) or Na (52% yield) a phosphine stabilized digermavinylidene (3) was isolated as crystalline material [<sup>Mes</sup>Nacnac = {[(Mes)NC(Me)]<sub>2</sub>CH}<sup>-</sup>, Mes = 2,4,6-Me<sub>3</sub>C<sub>6</sub>H<sub>2</sub>]. The electronic structure of the digermavinylidene was investigated by density functional theory calculations. In reaction with adamantylphosphaalkyne the product of a [2+2] cycloaddition was characterized (4). Adamantylazide abstracts at room temperature a germanium atom from the digermavinylidene and a tetrameric organogermanium nitride (5) was isolated as colorless crystals.
Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
2019-02-08

Abstract Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the performance of PfHRP2-based RDTs. A total of 400 samples collected from uncomplicated malaria cases from Kenya were investigated for the amino acid repeat profiles in exon 2 of pfhrp2 and pfhrp3 genes. In addition, PfHRP2 levels were measured in 96 individuals with uncomplicated malaria. We observed a unique distribution pattern of amino acid repeats both in the PfHRP2 and PfHRP3. 228 PfHRP2 and 124 PfHRP3 different amino acid sequences were identified. Of this, 214 (94%) PfHRP2 and 81 (65%) PfHRP3 amino acid sequences occurred only once. Thirty-nine new PfHRP2 and 20 new PfHRP3 amino acid repeat types were identified. PfHRP2 levels were not correlated with parasitemia or the number of PfHRP2 repeat types. This study shows the variability of PfHRP2, PfHRP3 and PfHRP2 concentration among uncomplicated malaria cases. These findings will be useful to understand the performance of PfHRP2-based RDTs in Kenya.
Torsional Osteotomy
2019-02-08

Abstract This article provides an overview of symptomatic torsional deformities of the lower extremity, and operative treatment techniques are described in detail. A definition of torsion versus rotation as well as information to physical examination and the relevance of radiological evaluation is given. Based on current literature and the own personal experience of the authors in osteotomies, surgical techniques at the proximal and at the distal femur, as well as at the tibia are presented.
Quantum waveguides with magnetic fields
2019-02-08

We study generalized quantum waveguides in the presence of moderate and strong external magnetic fields. Applying recent results on the adiabatic limit of the connection Laplacian we show how to construct and compute effective Hamiltonians that allow, in particular, for a detailed spectral analysis of magnetic waveguide Hamiltonians. We apply our general construction to a number of explicit examples, most of which are not covered by previous results.
Clinical spectrum of STX1B -related epileptic disorders
2019-02-09

These data expand the genetic and phenotypic spectrum of <i>STX1B</i>-related epilepsies to a diverse range of epilepsies that span the International League Against Epilepsy classification. Variants in <i>STX1B</i> are protean and contribute to many different epilepsy phenotypes, similar to <i>SCN1A</i>, the most important gene associated with fever-associated epilepsies.
Targeting CD39 Toward Activated Platelets Reduces Systemic Inflammation and Improves Survival in Sepsis: A Preclinical Pilot Study*
2019-02-09

Objectives: Sepsis is associated with a systemic inflammatory reaction, which can result in a life-endangering organ dysfunction. Pro-inflammatory responses during sepsis are characterized by increased activation of leukocytes and platelets, formation of platelet-neutrophil aggregates, and cytokine production. Sequestration of platelet-neutrophil aggregates in the microvasculature contributes to tissue damage during sepsis. At present no effective therapeutic strategy to ameliorate these events is available. In this preclinical pilot study, a novel anti-inflammatory approach was evaluated, which targets nucleoside triphosphate hydrolase activity toward activated platelets via a recombinant fusion protein combining a single-chain antibody against activated glycoprotein IIb/IIIa and the extracellular domain of CD39 (targ-CD39). Design: Experimental animal study and cell culture study. Setting: University-based experimental laboratory. Subjects: Human dermal microvascular endothelial cells 1, human platelets and neutrophils, and C57BL/6NCrl mice. Interventions: Platelet-leukocyte-endothelium interactions were evaluated under inflammatory conditions in vitro and in a murine lipopolysaccharide-induced sepsis model in vivo. The outcome of polymicrobial sepsis was evaluated in a murine cecal ligation and puncture model. To evaluate the anti-inflammatory potential of activated platelet targeted nucleoside triphosphate hydrolase activity, we employed a potato apyrase in vitro and in vivo, as well as targ-CD39 and as a control, nontarg-CD39 in vivo. Measurements and Main Results: Under conditions of sepsis, agents with nucleoside triphosphate hydrolase activity decreased platelet-leukocyte-endothelium interaction, transcription of pro-inflammatory cytokines, microvascular platelet-neutrophil aggregate sequestration, activation marker expression on platelets and neutrophils contained in these aggregates, leukocyte extravasation, and organ damage. Targ-CD39 had the strongest effect on these variables and retained hemostasis in contrast to nontarg-CD39 and potato apyrase. Most importantly, targ-CD39 improved survival in the cecal ligation and puncture model to a stronger extent then nontarg-CD39 and potato apyrase. Conclusions: Targeting nucleoside triphosphate hydrolase activity (CD39) toward activated platelets is a promising new treatment concept to decrease systemic inflammation and mortality of sepsis. This innovative therapeutic approach warrants further development toward clinical application.
A Workflow to Define, Map and Name a Carbonatite- or Alkaline Igneous-Associated REE-HFSE Mineral System: A Case Study from SW Germany
2019-02-09

Security of supply of “hi-tech” raw materials (including the rare earth elements (REE) and some high-field-strength elements (HFSEs)) is a concern for the European Union. Exploration and research projects mostly focus on deposit- to outcrop-scale description of carbonatite- and alkaline igneous-associated REE-HFSE mineralization. The REE-HFSE mineral system concept and approach are at a nascent stage, so developed further here. However, before applying the mineral system approach to a chosen REE-HFSE metallogenic province its mineral system extent first needs defining and mapping. This shifts a mineral system project’s foundation from the mineral system concept to a province’s mineral system extent. The mapped extent is required to investigate systematically the pathways and potential trap locations along which the REE-HFSE mass may be distributed. A workflow is presented to standardize the 4-D definition of a REE-HFSE mineral system at province-scale: (a) Identify and hierarchically organize a mineral system’s genetically related sub-divisions and deposits, (b) map its known and possible maximum extents, (c) name it, (d) discern its size (known mineral endowment), and (e) assess the favorability of the critical components to prioritize further investigations. The workflow is designed to generate process-based perspective and improve predictive targeting effectiveness along under-evaluated plays of any mineral system, for the future risking, comparing and ranking of REE-HFSE provinces and plays.
Stent‐retriever thrombectomy in the treatment of infants with acute thrombosis of the superior vena cava and innominate vein
2019-02-09

Abstract Objectives To describe the efficacy and safety of stent‐retriever thrombectomy in infants with thrombosis of the superior vena cava (SVC) and innominate vein. Background Thrombosis of the SVC and of the innominate vein is a potentially life threatening complication in infants during intensive care treatment following major surgical procedures. To avoid reoperations, we evaluated interventional revascularization by stent‐retriever thrombectomy. Methods From 2015 to 2017, five infants were diagnosed with acute thrombosis of the SVC and innominate vein following major cardiac or pediatric surgery. Using a femoral venous access and 4 or 5 French guiding catheters stent‐retriever systems (4/20 mm or 6/30 mm) were placed into the thrombus and retrieved under suction. We aimed to revascularize not only the SVC but also the innominate, jugular, and subclavian veins. Results Following repeated stent retrieving manoeuvers, we were able to reestablish flow in the major veins of all patients. Due to significant residual thrombotic material, we decided to perform additional balloon dilatation of the SVC and innominate vein in 3/5 patients. There were no complications related to the procedure and none of our patients required blood transfusion. Following the intervention, the patients received treatment with low‐molecular‐weight heparin. Interventional treatment achieved persistent patency of the SVC and innominate vein in all patients. Conclusion Stent‐retriever thrombectomy is a safe and effective method for interventional treatment of acute thrombosis of the central veins in infants. Due to the large amount of thrombotic material, it is frequently required to combine this method with balloon compression of residual thrombotic material.
Generation of two isogenic iPSC lines with either a heterozygous or a homozygous E280A mutation in the PSEN1 gene
2019-02-10

Alzheimer's disease (AD) is the most common form of dementia. Mutations in the gene PSEN1 encoding Presenilin1 are known to cause familial forms of AD with early age of onset. The most common mutation in the PSEN1 gene is the E280A mutation. iPSCs are an optimal choice for modeling AD, as they can be differentiated in vitro into neural cells. Here, we report the generation of two isogenic iPSC lines with either a homozygous or a heterozygous E280A mutation in the PSEN1 gene. The mutation was introduced into an iPSC line from a healthy individual using the CRISPR-Cas9 technology. Resource table.
Seeking certainty about Intolerance of Uncertainty: Addressing old and new issues through the Intolerance of Uncertainty Scale-Revised
2019-02-11

Intolerance of Uncertainty is a trans-diagnostic process that spans a range of emotional disorders and it is usually measured through the Intolerance of Uncertainty Scale-12. The current study aims at investigating some issues in the assessment of Intolerance of Uncertainty (IU) through the Italian Intolerance of Uncertainty Scale-Revised, a measure adapted from the Intolerance of Uncertainty Scale-12 to assess IU across the lifespan. In particular we address the factor structure among a large community sample, measurement invariance across gender, age, and over time, together with reliability and validity of the overall scale and its subscales. The questionnaire was administered to community (N = 761; mean age = 35.86 ± 14.01 years) and undergraduate (N = 163; mean age = 21.16 ± 2.64 years) participants, together with other self-report measures assessing constructs theoretically related to IU. The application of a bifactor model shows that the Italian Intolerance of Uncertainty Scale-Revised possesses a robust general factor, thus supporting the use of the unit-weighted total score of the questionnaire as a measure of the construct. Furthermore, measurement invariance across gender, age, and over time is supported. Finally, the Italian Intolerance of Uncertainty Scale-Revised appears to possess adequate reliability and validity. These findings support the unidimensionality of the measure, a conceptually reasonable result in line with the trans-diagnostic nature of Intolerance of Uncertainty. In addition, this study and comparison with published factor structures of the Intolerance of Uncertainty Scale-12 and of the Intolerance of Uncertainty Scale-Revised identify some issues for the internal structure of the measure. In particular, concern is expressed for the Prospective IU subscale. In light of the promising psychometric properties, the use of the Italian Intolerance of Uncertainty Scale-Revised as a univocal measure is encouraged in both research and clinical practice.
Detection of a quasi-periodic oscillation in γ-ray light curve of the high redshift blazar B2 1520+31
2019-02-11

We detected a possible quasi-periodic oscillation (QPO) of similar to 71 d in the 0.1-300 GeV gamma-ray Fermi-Large Area Telescope light curve of the high-redshift flat spectrum radio quasar B2 1520+31. We identify and confirm that quasi-period by Lomb-Scargle periodogram and weighted wavelet Z-transform analyses. Using this QPO period, and assuming it originates from accretion disc fluctuations at the innermost stable circular orbit, we estimate the central supermassive black hole mass to range between similar to 5.4 x 10(9) M-circle dot for a non-rotating black hole and similar to 3.4 x 10(10) M-circle dot for a maximally rotating black hole. We briefly discuss other possible radio-loud active galactic nuclei emission models capable of producing a gamma-ray QPO of such a period in a blazar.
ARR22 overexpression can suppress plant Two-Component Regulatory Systems
2019-02-11

In plants, several developmental processes are co-coordinated by cytokinins via phosphorylation dependent processes of the Two-Component System (TCS). An outstanding challenge is to track phosphorelay flow from cytokinin perception to its molecular outputs, of which gene activation plays a major role. To address this issue, a kinetic-based reporter system was expounded to track TCS phosphorelay activity in vivo that can distinguish between basal and cytokinin dependent effects of overexpressed TCS members. The TCS phosphorelay can be positively activated by cytokinin and inhibited by pharmaceuticals or naturally interfering components. In this case we took advantage of the phosphohistidine-phosphatase Arabidopsis Response Regulator (ARR) 22 and investigated its phosphocompetition with other TCS members in regulating promoters of ARR5 and WUS in Arabidopsis thaliana cell culture protoplasts. In congruency with the proposed function of ARR22, overexpression of ARR22 blocked the activation of all B-type ARRs in this study in a TCS dependent manner. Furthermore, this effect could not be mimicked by A-type response regulator overexpression or compensated by AHP overexpression. Compared to other reporter assays, ours mimicked effects previously observed only in transgenic plants for all of the TCS proteins studied, suggesting that it is possible to expose phosphocompetition. Thus, our approach can be used to investigate gene signaling networks involving the TCS by leveraging ARR22 as a TCS inhibitor along with B-type ARR overexpression.
World’s Fastest Brain-Computer Interface: Combining EEG2Code with Deep Learning
2019-02-11

Abstract In this paper, we present a Brain-Computer Interface (BCI) that is able to reach an information transfer rate (ITR) of more than 1200 bit/min using non-invasively recorded EEG signals. By combining the EEG2Code method with deep learning, we present an extremely powerful approach for decoding visual information from EEG. This approach can either be used in a passive BCI setting to predict properties of a visual stimulus the person is viewing, or it can be used to actively control a BCI spelling application. The presented approach was tested in both scenarios and achieved an average ITR of 701 bit/min in the passive BCI approach with the best subject achieving an online ITR of 1237 bit/min. The presented BCI is more than three times faster than the previously fastest BCI and allows to discriminate 500,000 different visual stimuli based on 2 seconds of EEG data with an accuracy of up to 100 %. When using the approach in an asynchronous BCI for spelling, we achieved an average utility rate of 175 bit/min, which corresponds to an average of 35 error-free letters per minute. As we observe a ceiling effect where more powerful approaches for brain signal decoding do not translate into better BCI control anymore, we discuss if BCI research has reached a point where the performance of non-invasive BCI control cannot be substantially improved anymore.
Ancient RNA from Late Pleistocene permafrost and historical canids shows tissue-specific transcriptome survival
2019-02-11

Abstract While sequencing ancient DNA from archaeological material is now commonplace, very few attempts to sequence ancient transcriptomes have been made, even from typically stable deposition environments such as permafrost. This is presumably due to assumptions that RNA completely degrades relatively quickly, particularly when dealing with autolytic, nuclease-rich mammalian tissues. However, given the recent successes in sequencing ancient RNA (aRNA) from various sources including plants and animals, we suspect that these assumptions may be incorrect or exaggerated. To challenge the underlying dogma, we generated shotgun RNA data from sources that might normally be dismissed for such study. Here we present aRNA data generated from two historical wolf skins, and permafrost-preserved liver tissue of a 14,300-year-old Pleistocene canid. Not only is the latter the oldest RNA ever to be sequenced, but also shows evidence of biologically relevant tissue-specificity and close similarity to equivalent data derived from modern-day control tissue. Other hallmarks of RNA-seq data such as exon-exon junction presence and high endogenous ribosomal RNA content confirms our data’s authenticity. By performing independent technical replicates using two high-throughput sequencing platforms, we show not only that aRNA can survive for extended periods in mammalian tissues, but also that it has potential for tissue identification, and possibly further uses such as in vivo genome activity and adaptation, when sequenced using this technology.