julilili42/papers huggingface dataset:
Combination of Single-Grain Fission-Track Chronology and Morphological Analysis of Detrital Zircon Crystals in Provenance Studies: Sources of the Macigno Formation (Apennines, Italy)
2001-07-01

Fission track (FT) analyses on unannealed detrital min- erals provide a powerful tool both for refining provenance models de- rived from traditional methods and for collecting information about erosion rates of the source area. Their power is increased if they are coupled with the study of zircon morphology. This combination of methods is applied to the Chattian-Aquitanian (25-23 Ma) Macigno turbidite complex. Basin-fill patterns and petrographical studies con- sistently identify the uplifting western Central Alps as the main source region for the Macigno Formation. Most zircon grains fall into a young age cluster ( ; 40-30 Ma), de- rived from a rapidly exhuming crystalline source region with a high cooling rate. Within this cluster, two age subgroups can be distin- guished at 30 and 40 Ma. In the younger subgroup, the zircon mor- phology supports the presence of two main populations: (1) from ig- neous rocks (S-type euhedral zircons), which appear to be partly de- rived from airborne tuffs; and (2) from metasedimentary units. In huge volumes of these metamorphic rocks, mica Ar-Ar and zircon fission- track thermochronometers have been reset, because of high geothermal gradients in the vicinity of the Periadriatic intrusives in mid-Oligocene times. At the present surface of the Alps, zircon FT ages around and slightly less than 30 Ma are reported in the Sesia-Lanzo zone, the Gran Paradiso Massif, the Upper Pennine nappes, the Monte Rosa Massif, and the Dent Blanche complex. The older subgroup of the Tertiary zircons (40 Ma) may have been supplied by metamorphic and mig- matitic rocks affected by an Eocene high-temperature phase. A Late Cretaceous age cluster ( ; 70-60 Ma) is related to cooling after the main Austroalpine metamorphic event at 110-100 Ma. Most of the recently exposed Austroalpine nappe complex displays mica cooling ages and zircon FT ages between 95-70 Ma and 99-55 Ma, respectively. Finally, an ill-defined Jurassic age cluster, with a mean in Late Ju- rassic times, is related to rift-shoulder heating of the Austroalpine/ South-Alpine crystalline basement due to rifting of the Pennine oceanic domain. Presently, the Silvretta nappe complex, situated at the western termination of the Austroalpine realm, and the South-Alpine basement west of the Canavese Line, display similar zircon FT ages. Therefore, a westward continuation of the Silvretta complex prior to deep Neo- gene erosion is suggested.
Oxygenator thrombosis: worst case after development of an abnormal pressure gradient - incidence and pathway
2001-07-01

The development of an abnormally high pressure gradient (APG) before the membrane oxygenator (MO) is a complication that occurs during some extracorporeal circulation (ECC) procedures. The present study deals with the incidence of an APG and discusses a probable causative pathway by comparing surface-coated and uncoated oxygenation systems. Five thousand six hundred and seventeen adult ECCs were carried out (2581 without and 3036 with surface coatings). The incidence of an APG, therefore, amounted to 0.03% in the group with coated systems and 4.3% in the uncoated group. In addition, an in vitro study demonstrated significantly reduced adhesion and activation of platelets and leucocytes when the surfaces of the MOs were coated with heparin or polypeptides. The advantages of coating surfaces of ECC devices possibly depend on the selective adsorption of particular plasma proteins. These will presumably form a biocompatible membrane on the surface, and minimize pathological deposit of fibrin, platelets and other blood cells, and, therefore, implicate the prevention of an oxygenator failure.
Excretion of two proteases by the ectomycorrhizal fungus Amanita muscaria
2001-07-01

Abstract In the soils of mature forests, nitrogen availability is mainly the result of litter decomposition. Thus protein degradation is of major interest for nutrition. Two aspartic proteases were excreted in a pH‐dependent manner by the ectomycorrhizal fungus Amanita muscaria grown in liquid culture. AmProt1 with a molecular mass of approximately 45 kDa was mainly present at pH values up to 5·4, whereas the excretion of AmProt2 with a molecular mass of about 90 kDa was only detectable at pH values between pH 5·4 and 6·3. In addition, the pH optima of both enzymes differed significantly. AmProt1 had a narrow pH optimum around 3, whereas AmProt2 had a broad pH optimum between 3 and 5·5 and a higher affinity to the substrate methylcasein. One cDNA‐clone ( AmProt1 *) that presumably encodes AmProt1 was identified. Like AmProt1, this cDNA was expressed in a pH‐dependent manner. In addition, carbohydrate and to a lesser extent nitrogen depletion significantly enhanced AmProt1 * expression. In fully developed Populus hyb./ A. muscaria ectomycorrhizas the expression of AmProt1 * was significantly higher in hyphae of the Hartig net compared with those of the fungal sheath. The role of AmProt1 and AmProt2 for fungal physiology and competitiveness is discussed.
The Steady‐State Dipole‐Flow Test for Characterization of Hydraulic Conductivity Statistics in a Highly Permeable Aquifer: Horkheimer Insel Site, Germany
2001-07-01

Abstract Over the last decade the dipole‐flow test (DFT) evolved from the general idea of using recirculatory flow to evaluate aquifer properties, to the development of prototype instrumentation and feasibility studies, to a reliable tool for characterization of aquifer heterogeneity. The DFT involves the interpretation of head in recirculatory flow between injection and extraction sections (chambers) in a single well isolated from each other by a multipacker system. In this study, the steady‐state dipole flow test (DFT) has been used to characterize the statistics of horizontal hydraulic conductivity (K r ) of the highly permeable, heterogeneous, and thin aquifer at the Horkheimer Insel site, Germany. In previous studies, K r estimates were based on the steady‐state head difference between chambers. A new by‐chamber interpretation is proposed that is based on drawdown within each individual chamber. This interpretation yields more detailed information on structure of heterogeneity of the aquifer without introducing complexity into the analysis. The DFT results indicate that K r ranges from 49 to 6000 m/day (mean In K r [(m/s)]∼—4, and variance of In K r [(m/s)] ∼ 1–2). Descriptive statistics from the DFT compare well with those from previous field and laboratory tests (pumping, borehole flowmeter, and permeameter tests and grain‐size analysis) at this site. It is shown that the role of confining boundaries in the DFT interpretation is negligible even in this case of a thin (&lt; 4 m thick) aquifer. This study demonstrates the flexibility of the DFT and expands the potential application of this method to a wide range of hydrogeologic settings.
Evaluation of Glaucomatous Visual Field Loss with Locally Condensed Grids Using Fundus-Oriented Perimetry (FOP)
2001-07-01

Purpose We compared detection rates of glaucomatous visual field defects (VFDs) between a conventional rectangular stimulus grid and locally condensed test point arrangements in morphologically suspicious regions. Methods Humphrey Field Analyzer model 630 (HFA I, program 30-2 with a rectangular 6° × 6° grid) was used as the conventional perimetric method. Individual local test-point condensation was realized by fundus-oriented perimetry (FOP) on the Tuebingen Computer Campimeter (TCC). Results Of a total of 66 glaucoma patients, or suspected sufferers, 23 showed normal findings and 27 showed pathological findings with both methods. In 15 cases we found normal visual fields in HFA 30-2, whereas FOP revealed early glaucomatous functional damage. Only one case showed pathological HFA results, while FOP was normal. Detection rates of VFDs significantly differed between the two methods (p &lt; 0.001; sign test). Conclusions FOP, using individually condensed test grids, significantly increases detection rates of glaucomatous VFDs in morphologically suspicuous areas compared with a conventional HFA 30-2 technique using equidistant rectangular (6° × 6°) test point arrangements.
Thermoradiotherapy for locally recurrent breast cancer with skin involvement
2001-07-01

This study showed that, in extensively pre-treated patients with locally recurrent breast cancer, local tumour control after thermoradiotherapy depended on tumour resectability, response of macroscopic tumour to thermoradiotherapy, and total irradiation dose.
Na+/HCO3- cotransport in normal and cystic fibrosis intestine.
2001-07-01

In a search for the HCO(3)(-) supply mechanisms to the enterocyte we cloned and sequenced an intestinal subtype of the Na(+)HCO(3)(-) cotransporter isoform I (dNBC1), which turned out to be identical to the pancreatic NBC1 subtype (pNBC1). Within the intestine, we found particularly high NBC1 expression levels in the duodenum and proximal colon. Experiments with stripped rabbit duodenum in Ussing-chambers revealed that Na(+)HCO(3)(-) cotransport (NBC) and CO(2) hydration/Na(+)/H(+) exchange were equally important duodenal HCO(3)(-) supply pathways and were both upregulated during cAMP-mediated secretion. In the proximal colon, however, HCO(3)(-) secretion was low but NBC1 expression even higher than in the duodenum. Ussing-chamber experiments with an NBC-specific inhibitor revealed that NBC, coupled to basolateral Cl(-)/HCO(3)(-) exchange, was an important alternative Cl(-) supply pathway to Na(+)K(+)2Cl(-) cotransport (NKCC) during cAMP-stimulated colonic Cl(-) secretion. To investigate the functional integrity of anion uptake pathways in the absence of cystic fibrosis transmembrane conductance regulator (CFTR), we fluorometrically assessed NBC and NKCC transport rates and cell volume before and during forskolin-stimulation in isolated colonic crypts from normal and CFTR (-/-) mice. Although forskolin stimulation decreased cell volume only in normal, not in CFTR (-/-) crypts, it activated NBC and NKCC to a similar degree in both normal and CFTR (-/-) crypts. We conclude that, depending on the intestinal segment, NBC1 plays an important role in basolateral HCO(3)(-) or Cl(-) uptake. Expression and activation by cAMP is preserved in CFTR (-/-) intestine.
Synthesis and Bioactivities of Naturally Occurring Anthraquinones: Isochrysophanol, Isozyganein, ω-Hydroxyisochrysophanol and Morindaparvin
2001-07-01

Isochrysophanol, isozyganein, ω-hydroxyisochrysophanol, and morindaparvin are naturally occurring substituted anthraquinones. We report the synthesis of these compounds as well as selected biological activities.
Effect of the Pattern of Elevated Free Fatty Acids on Insulin Sensitivity and Insulin Secretion in Healthy Humans
2001-07-01

In order to investigate whether the pattern of elevated free fatty acids (FFAs) has any effect on insulin sensitivity and insulin secretion in humans, we produced 2 distinct serum FFA patterns (PT 1 and 2) by infusing 6 healthy volunteers with 2 different lipid emulsions plus heparin for 24 hours. A hyperglycemic clamp (approx. 8 mM, 140 min) was performed before and 5 and 24 hours after both lipid infusions to determine insulin sensitivity and insulin secretion simultaneously. Total FFAs had increased comparably by 24 hours (2020+/-268 microM in PT 1) and (1812+/-154 microM in PT 2, p =0.24). Serum PT 1 contained 66% saturated FFAs plus monoenes and 34% polyenes, while PT 2 contained 80% saturated FFAs plus monoenes and 20% polyenes. Thus, the ratio of polyunsaturated to saturated plus monoenes was about 0.5 in PT 1 vs. 0.25 in PT 2. In PT 1, the insulin sensitivity index (ISI) decreased by 20 +/- 7% and 27 +/- 10% from basal after 5 and 24 hours, respectively. In PT 2, the ISI decreased significantly more after 5 (41+/-7%, p = 0.008) and 24 hours (52+/-6%, p = 0.005). In contrast, different phases of insulin secretion did not change during the lipid infusion and did not vary between the two FFA profiles. In conclusion, these findings provide preliminary evidence that the composition of elevated serum FFAs influenced insulin sensitivity in humans. The FFA pattern low in polyunsaturated FFAs reduced insulin sensitivity more than the pattern high in polyunsaturated FFAs. In contrast, no effect on insulin secretion was observed.
Postprandial neuronal activation in the nucleus of the solitary tract is partly mediated by CCK-A receptors
2001-07-01

CCK-A receptors and neurons of the nucleus of the solitary tract (NTS) are involved in the regulation of food intake, and in rats, there is evidence for involvement of an intestinal vagal afferent pathway. Studies investigating the role of CCK-A receptors in activation of NTS neurons using highly selective CCK-A receptor agonists and antagonists have yielded conflicting data. In the present study, we investigated CCK-induced and postprandial activation of NTS neurons, together with food intake studies, in CCK-A receptor-deficient Otsuka Long-Evans Tokushima fatty (OLETF) rats. Activated NTS neurons were detected using immunohistological staining for c-Fos protein. Exogenous CCK increased the number of c-Fos protein-positive cells in the NTS of Sprague-Dawley and CCK-A receptor-intact Long-Evans Tokushima Otsuka (LETO) rats but had no effect in CCK-A receptor-deficient OLETF rats. Food intake-induced c-Fos protein expression in NTS neurons was significantly reduced in CCK-A receptor-deficient OLETF rats compared with Sprague-Dawley or LETO rats. Postprandial c-Fos protein expression in the NTS was also significantly decreased after pretreatment with the CCK-A receptor antagonist MK329 after both short- and long-term fasting periods. Exogenous CCK decreased cumulative food intake in Sprague-Dawley and LETO rats but not in OLETF rats. These data demonstrate that CCK-A receptors are involved in the CCK- and food-induced c-Fos protein expression in the NTS. Taken together with the results of the food intake studies, this suggests that activation of CCK-A receptors is involved in the postprandial activation of NTS neurons and in the regulation of food intake.
Nuclear Peroxisome Proliferator-Activated Receptors α and γ Have Opposing Effects on Monocyte Chemotaxis in Endometriosis
2001-07-01

The peroxisome proliferator-activated receptors (PPARs) alpha and gamma are nuclear receptors that play important roles in inflammatory diseases like ulcerative colitis and arthritis. In this study, we examined the possible role of PPARs in macrophage attraction into the peritoneal cavity of patients with endometriosis. We identified PPAR-alpha and -gamma messenger RNA by RT-PCR and protein by immunoblotting of lysates of peritoneal macrophages and monocytic U937 cells. Using immunocytochemistry, we localized PPAR-alpha and -gamma within the nuclei of both cell types. Monocyte chemotactic activity of peritoneal fluid from patients with endometriosis was quantified in Boyden chambers. Migration of U937 cells was increased by WY 14643 and reduced by rosiglitazone. Peritoneal fluid from patients with endometriosis activated U937 cells transiently transfected with a PPAR-alpha/GAL4 luciferase reporter. By contrast, peritoneal fluid did not cause significant activation of PPAR-gamma/GAL4 constructs. The U937 cells transiently transfected with a PPAR response element luciferase reporter showed disease stage-dependent up-regulation when treated with peritoneal fluid from patients with endometriosis. Treatment with peritoneal fluid from healthy controls down-regulated PPAR response element transactivation. We conclude that peritoneal fluid of endometriosis patients contains activators of PPAR-alpha that stimulate macrophage chemotaxis. Inhibitors of PPAR-alpha or activators of PPAR-gamma could be developed for the treatment of inflammation associated with endometriosis.
Determination of thecis-trans isomerization barrier of severalL-peptidyl-L-proline dipeptides by dynamic capillary electrophoresis and computer simulation
2001-07-01

Dynamic capillary electrophoresis (DCE) and computer simulation of the elution profiles with the theoretical plate and the stochastic model has been applied to determine the isomerization barriers of the three dipeptides L-alanyl-L-proline, L-leucyl-L-proline, and L-phenylalanyl-L-proline. The separation of the rotational cis-trans isomers has been performed in an aqueous 70 mM borate buffer at pH 9.5. Interconversion profiles featuring plateau formation and peak broadening were observed. To determine the rate constants k1 and k(-1) of the cis-trans isomerization in dynamic capillary electrophoresis, equations have been derived for the theoretical plate model and stochastic model. The electropherograms were simulated with the ChromWin software which uses the experimental data plateau height h(plateau), peak width at half height Wh, the total migration times of the cis-trans isomers tR and the electroosmotic break-through time t0 as well as the peak ratio [cis]/[trans]. From temperature-dependent measurements, the rate constants k1 and k(-1) and the kinetic activation parameters deltaG#, deltaH# and deltaS# of the cis-trans isomerization of the three dipeptides were obtained.
Enantiomer separation by nonaqueous and aqueous capillary electrochromatography on cyclodextrin stationary phases
2001-07-01

Native beta- and gamma-cyclodextrin bound to silica (ChiraDex-beta and ChiraDex-gamma) were packed into capillaries and used for enantiomer separation by capillary electrochromatography (CEC) under aqueous and nonaqueous conditions. Negatively charged analytes (dansyl-amino acids) were resolved into their enantiomers by nonaqueous CEC (NA-CEC). The addition of a small amount of water to the nonaqueous mobile phase enhanced the enantioselectivity but increased the elution time. The choice of the background electrolyte (BGE) determined the direction of the electroosmotic flow (EOF). With 2-(N-morpholino) ethanesulfonic acid (MES) or triethylammonium acetate (TEAA) as BGE an inverse EOF (anodic EOF) was observed while with phosphate a cathodic EOF was found. The apparent pH (pH*), the concentration of the BGE, and the nature of the mobile phase strongly influenced the elution time, the theoretical plate number and the chiral separation factor of racemic analytes.
Molecular Characterization of the 4′-Phosphopantothenoylcysteine Decarboxylase Domain of Bacterial Dfp Flavoproteins
2001-07-01

The NH(2)-terminal domain of the bacterial flavoprotein Dfp catalyzes the decarboxylation of (R)-4'-phospho-N-pantothenoylcysteine to 4'-phosphopantetheine, a key step in coenzyme A biosynthesis. Dfp proteins, LanD proteins (for example EpiD, which is involved in epidermin biosynthesis), and the salt tolerance protein AtHAL3a from Arabidopsis thaliana are homooligomeric flavin-containing Cys decarboxylases (HFCD protein family). The crystal structure of the peptidyl-cysteine decarboxylase EpiD complexed with a pentapeptide substrate has recently been determined. The peptide is bound by an NH(2)-terminal substrate binding helix, residue Asn(117), which contacts the cysteine residue of the substrate, and a COOH-terminal substrate recognition clamp. The conserved motif G-G/S-I-A-X-Y-K of the Dfp proteins aligns partly with the substrate binding helix of EpiD. Point mutations within this motif resulted in loss of coenzyme binding (G14S) or in significant decrease of Dfp activity (G15A, I16L, A17D, K20N, K20Q). Exchange of Asn(125) of Dfp, which corresponds to Asn(117) of EpiD, and exchange of Cys(158), which is within the proposed substrate recognition clamp of Dfp, led to inactivity of the enzyme. Molecular analysis of the conditional lethality of the Escherichia coli dfp-707 mutant revealed that the single point mutation G11D of Dfp is related to decreased amounts of soluble Dfp protein at 37 degrees C.
Colacosiphon: a new genus described for a mycoparasitic fungus
2001-07-01

An undescribed heterobasidiomycetous fungus was found in cultures originating from bark beetles placed onto a natural medium. Spores were borne apically on extraordinarily long, filiform, aseptate structures arising from swollen hyphal cells. Within these cells, parasitic interactions with hyphae of an unidentified ascomycete by colacosomes were found. The septal pores of the parasitic fungus were simple and associated with microbodies containing inclusions similar to those of Woronin bodies. The fungus is closely related to Cryptomycocolax abnormis, the single species of the hitherto monotypic order Cryptomycocolacales. The undescribed fungus differs from C. abnormis by the presence of only one type of colacosome, the absence of clamps, and the absence of a transversely septate stage during sporogenesis. A new genus and species is proposed for the undescribed fungus: Colacosiphon filiformis. Since it is not clear whether the unusual sporogenous structures are conidiophores or basidia, the new taxon was preliminarily considered as anamorphic.
Impaired Non-Esterified Fatty Acid Suppression is Associated with Endothelial Dysfunction in Insulin Resistant Subjects
2001-07-01

In a recent study, we found a significant association between insulin resistance (IR) and disturbed flow-associated (endothelial-dependent) vasodilation in first-degree relatives of subjects with type 2 diabetes. However, the mechanisms linking insulin resistance and endothelial dysfunction (ED) have not been fully elucidated. Experimental data have pointed out that non-esterified fatty acids (NEFA) have a modulating effect on NO-synthase activity, and therefore on endothelial function. The aim of our study was to evaluate whether insulin resistance associated impaired NEFA suppression is present in subjects with ED. We examined 53 first-degree relatives (FDR) of patients with type 2 diabetes (32f, 21 m, mean age 35 years). Endothelial function was measured as flow-associated vasodilation (FAD%) of the brachial artery. Insulin sensitivity was evaluated with a standard hyperinsulinemic glucose clamp (insulin infusion rate of 1 mU/kg/min). While under fasting conditions, NEFA did not differ between groups with high or low FAD (0.415+/-0.033 vs. 0.394 +/- 0.040 mmol/l; p = n. s.), reduced FAD% was significantly associated with higher non-esterified fatty acids concentrations during steady state of the glucose clamp (0.072+/-0.022 vs. 0.039+/-0.016mmol/l; p=0.04). This association was independent of insulin levels under fasting conditions and during the glucose clamp. In conclusion, our results reveal a significant association between endothelial dysfunction and impaired non-esterified fatty acid suppression in insulin resistant subjects. As insulin resistance of lipolysis is a feature of the insulin resistance syndrome, these results suggest that elevated NEFA concentrations could play a role linking endothelial dysfunction and insulin resistance in vivo.
Cooper pair and spin fluctuations in underdoped high-temperature superconductors
2001-07-01

We have calculated the quasiparticle self-energy Σ(k,ω), spectral function N(k,ω), and density of states N(ω) due to exchange of spin and d-wave pair fluctuations above Tc in the FLEX (fluctuation exchange) approximation. We find that for T → Tc a pseudogap evolves in N(ω) which is similar to the structure caused by the superconducting d-wave gap below Tc. A satellite peak occurs in N(k,ω) for k near (π,0) which is caused by a peak in − ImΣ(k,ω). Since these effects become relatively weak at higher temperatures T ≃ 2Tc, however, we have to conclude that fluctuations of the pair amplitude within this spin fluctuation pairing scenario cannot explain the observed pseudogap behavior well above Tc in underdoped cuprates.