123 ASSESSMENT OF MOLECULAR DIFFERENCES BETWEEN EARLY AND LATELY DEVELOPED RENAL CELL CARCINOMA
2011-04-01

You have accessJournal of UrologyKidney Cancer: Basic Research1 Apr 2011123 ASSESSMENT OF MOLECULAR DIFFERENCES BETWEEN EARLY AND LATELY DEVELOPED RENAL CELL CARCINOMA Fabian Janke, Susanne Fuessel, Joerg Hofmann, Matthias Meinhardt, Arndt Hartmann, Bastian Gunawan, Christine G. Stoehr, Sabine Brookman-May, Frank Becker, Jens Bedke, Karen Bluemke, Paolo Fornara, Mieczyslaw Gajda, Volker Jung, Axel S. Merseburger, Arne Strauss, Bernhard Walter, Heiko Wunderlich, Manfred P. Wirth, and Kerstin Junker Fabian JankeFabian Janke Dresden, Germany More articles by this author , Susanne FuesselSusanne Fuessel Dresden, Germany More articles by this author , Joerg HofmannJoerg Hofmann Dresden, Germany More articles by this author , Matthias MeinhardtMatthias Meinhardt Dresden, Germany More articles by this author , Arndt HartmannArndt Hartmann Erlangen, Germany More articles by this author , Bastian GunawanBastian Gunawan Goettingen, Germany More articles by this author , Christine G. StoehrChristine G. Stoehr Erlangen, Germany More articles by this author , Sabine Brookman-MaySabine Brookman-May Regensburg, Germany More articles by this author , Frank BeckerFrank Becker Homburg, Germany More articles by this author , Jens BedkeJens Bedke Tuebingen, Germany More articles by this author , Karen BluemkeKaren Bluemke Halle, Germany More articles by this author , Paolo FornaraPaolo Fornara Halle, Germany More articles by this author , Mieczyslaw GajdaMieczyslaw Gajda Jena, Germany More articles by this author , Volker JungVolker Jung Homburg, Germany More articles by this author , Axel S. MerseburgerAxel S. Merseburger Hannover, Germany More articles by this author , Arne StraussArne Strauss Goettingen, Germany More articles by this author , Bernhard WalterBernhard Walter Erlangen, Germany More articles by this author , Heiko WunderlichHeiko Wunderlich Jena, Germany More articles by this author , Manfred P. WirthManfred P. Wirth Dresden, Germany More articles by this author , and Kerstin JunkerKerstin Junker Jena, Germany More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.189AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Aim of the study was to identify molecular factors that might determine whether renal cell carcinoma (RCC) develop early or rather lately during lifetime. For this, several well-known prognostic markers were selected, such as the inhibitors of apoptosis survivin and XIAP, the proliferation marker Ki-67, carbonic anhydrase 9 (CA9) and microvessel density (MVD). Expression levels of the growth factor receptors KDR, PDGFRA and PDGFRB were additionally evaluated in the group of young (≤35 years) and of old RCC patients (≥80 years). METHODS Tissues specimens of 205 RCC patients at the age of ≤35 years (n=58) or ≥80 years (n=147) who underwent nephrectomy between 1990 and 2006 were collected at pathology and urology departments of the network partners. Tissue microarrays generated from representative tumor areas were histopathologically reviewed by two uropathologists according to the 2002 TNM & the 2004 WHO classification. Immunohistochemical stainings were assessed separately for nuclear and cytoplasmatic markers. The MVD was analyzed by counting CD34-positive vessels. Expression levels were determined using suitable scoring systems and dichotomized using the median as cut-off value for statistical analyses. Each marker was evaluated in dependence on tumor stage, WHO grading and RCC subtype. Detailed statistics were performed for clear-cell RCC (ccRCC) that represented the predominant subtype in both age groups. RESULTS Expression behavior of all markers clearly differed between ccRCC and the other RCC subtypes. Due to the low percentage of other subtypes reliable statistics were only possible for ccRCC. Significantly lower CA9 expression was observed in ccRCC samples from young patients. MVD was significantly higher in specimens of the young ccRCC patients. XIAP showed no differential expression whereas survivin displayed an increased nuclear staining in high grade ccRCC of young patients and Ki-67 in high grade ccRCC of old patients. None of the markers showed an association to tumor stage. Expression of KDR, PDGRFA and PDGFRB was not significantly altered in relation to age, T stage or grade. CONCLUSIONS MVD and CA9 are the only markers with a statistically significant difference in immunohistochemical staining between the tumor tissues of the examined age groups. Since it is expected that RCC patients with a low CA9 expression have a less favorable prognosis, the lower CA9 expression levels in the group of young patients could be understood as an indicator for the aggressiveness of ccRCC with an early-onset. This has to be proven in the future on the basis of follow-up data. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e52 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Fabian Janke Dresden, Germany More articles by this author Susanne Fuessel Dresden, Germany More articles by this author Joerg Hofmann Dresden, Germany More articles by this author Matthias Meinhardt Dresden, Germany More articles by this author Arndt Hartmann Erlangen, Germany More articles by this author Bastian Gunawan Goettingen, Germany More articles by this author Christine G. Stoehr Erlangen, Germany More articles by this author Sabine Brookman-May Regensburg, Germany More articles by this author Frank Becker Homburg, Germany More articles by this author Jens Bedke Tuebingen, Germany More articles by this author Karen Bluemke Halle, Germany More articles by this author Paolo Fornara Halle, Germany More articles by this author Mieczyslaw Gajda Jena, Germany More articles by this author Volker Jung Homburg, Germany More articles by this author Axel S. Merseburger Hannover, Germany More articles by this author Arne Strauss Goettingen, Germany More articles by this author Bernhard Walter Erlangen, Germany More articles by this author Heiko Wunderlich Jena, Germany More articles by this author Manfred P. Wirth Dresden, Germany More articles by this author Kerstin Junker Jena, Germany More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...