MP40-09 ESTABLISHMENT OF A NEW LARGE ANIMAL MODEL FOR STRESS URINARY INCONTINENCE USING GERMAN LANDRACE PIGS
2017-04-01

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Female Incontinence: Therapy I1 Apr 2017MP40-09 ESTABLISHMENT OF A NEW LARGE ANIMAL MODEL FOR STRESS URINARY INCONTINENCE USING GERMAN LANDRACE PIGS Anika Albrecht, Alexandra Kelp, Bastian Amend, Mario Kluender, Arnulf Stenzl, and Wilhelm K. Aicher Anika AlbrechtAnika Albrecht More articles by this author , Alexandra KelpAlexandra Kelp More articles by this author , Bastian AmendBastian Amend More articles by this author , Mario KluenderMario Kluender More articles by this author , Arnulf StenzlArnulf Stenzl More articles by this author , and Wilhelm K. AicherWilhelm K. Aicher More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.1256AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Stress urinary incontinence (SUI) is a serious problem amongst many women especially after giving child birth. In order to find a better treatment we established a large animal model displaying features of this disease. After evaluation of these animals the long-term goal will be to include this model in different preclinical projects to explore improved or novel methods for SUI diagnosis and therapy. METHODS In this study, 9 female German landrace pigs (approx. 20 kg) were included. To induce incontinence group 1 (3 pigs) underwent dilatation of the bladder sphincter for 5 minutes using an 18 Chr balloon catheter filled with 20 ml liquid. Group 2 was treaded with an electro cautery by applying four distinct spots 1 cm from the bladder neck (10 sec, 16 Watt). Group 3 received a combination of dilatation and distal cauterisation (approx. 5 cm from the bladder neck). After 3 weeks standard (s-UPP) and high-definition urethral pressure profilometry (HD-UPP) were performed, the animals were sacrificed and the bladder tissue was harvested for cryo sectioning. AZAN and antibody stainings with reagents to desmin and fast myosin were performed to compare urethra from treated animals with control tissue. RESULTS Immediately after treatment animals of all three groups showed a lower s-UPP or HD-UPP compared to the profiles obtained bevor surgical intervention. Only the urethral wall pressure of animals that received dilatation and cauterisation remained reduced after 3 weeks of observation. Animals from group 1 and 2 regenerated just fine and the UPP was even higher than before surgical intervention. AZAN staining and antibody stainings with desmin and fast myosin showed some morphological differences of the muscle tissue in animals of group 3 compared to group 1, 2 and the control. Additionally, signs of electro cautery were clearly visible in group 3. CONCLUSIONS The study demonstrated that only dilatation combined with distal cauterisation of the urethra led to incontinent animals, whereas dilatation or proximal cauterisation alone showed no differences in the UPP after 3 weeks of observation. This animal model may represent a good source for further experiments to investigate improved methods for diagnosis or novel therapies for a damaged sphincter muscle. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e526 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Anika Albrecht More articles by this author Alexandra Kelp More articles by this author Bastian Amend More articles by this author Mario Kluender More articles by this author Arnulf Stenzl More articles by this author Wilhelm K. Aicher More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...