julilili42/papers huggingface dataset:
Impact of Four Protein Additives in Cryogels on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells
2019-08-07

Human adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) have great potential for bone tissue engineering. Cryogels, mimicking the three-dimensional structure of spongy bone, represent ideal carriers for these cells. We developed poly(2-hydroxyethyl methacrylate) cryogels, containing hydroxyapatite to mimic inorganic bone matrix. Cryogels were additionally supplemented with different types of proteins, namely collagen (Coll), platelet-rich plasma (PRP), immune cells-conditioned medium (CM), and RGD peptides (RGD). The different protein components did not affect scaffolds’ porosity or water-uptake capacity, but altered pore size and stiffness. Stiffness was highest in scaffolds with PRP (82.3 kPa), followed by Coll (55.3 kPa), CM (45.6 kPa), and RGD (32.8 kPa). Scaffolds with PRP, CM, and Coll had the largest pore diameters (~60 μm). Ad-MSCs were osteogenically differentiated on these scaffolds for 14 days. Cell attachment and survival rates were comparable for all four scaffolds. Runx2 and osteocalcin levels only increased in Ad-MSCs on Coll, PRP and CM cryogels. Osterix levels increased slightly in Ad-MSCs differentiated on Coll and PRP cryogels. With differentiation alkaline phosphatase activity decreased under all four conditions. In summary, besides Coll cryogel our PRP cryogel constitutes as an especially suitable carrier for bone tissue engineering. This is of special interest, as this scaffold can be generated with patients’ PRP.
Platelet dysfunction caused by a novel thromboxane A2 receptor mutation and congenital thrombocytopenia in a case of mild bleeding
2019-08-07

Chronic hemorrhagic diathesis in patients showing normal levels of plasmatic clotting factors strongly suggests for congenital platelet disorders. We report on a pediatric patient (male, 3 years, D1) with mild bleeding. A sibling (D2), his mother (D3) and father (D4) were included for laboratory investigation. Platelet counts in D1, D2 and D4 indicated mild thrombocytopenia (100 Gpt/L). D1 and D3 platelets showed significantly diminished aggregation response on arachidonic acid and U46619 stimulation. Immunostaining for platelet proteins on blood smears of D1 and D2 indicated defects in ß1-tubulin. Exon sequencing of <i>TBXA2R</i> and <i>TUBB1</i> revealed heterozygosity for the novel <i>TBXA2R*c.908T>C</i> (p.L303P) mutation in D1 and D3. <i>TUBB1</i> was either wild type (D2, D3) or heterozygous (D1, D4) for the common polymorphism <i>TUBB1*c.920G>A</i> (rs6070697; p.R307H). In conclusion, the bleeding phenotype of the index patient can be explained by a diminished platelet function caused by the <i>TBXA2R*c.908T>C</i> mutation inherited from the mother and a mild thrombocytopenia with unknown molecular basis that is inherited from the father.
The Verbal Interaction Social Threat Task: A New Paradigm Investigating the Effects of Social Rejection in Men and Women
2019-08-07

In recent years, digital communication and social media have taken an indispensable role in human society. Social interactions are no longer bound to real-life encounters, but more often happen from behind a screen. Mimicking an online communication platform, we developed a new, fMRI compatible, social threat paradigm to investigate sex differences in reactions to social rejection. During the Verbal Interaction Social Threat Task (VISTTA), participants initiate 30 short conversations by selecting one of four predefined opening sentences. Two computerized interlocutors respond to the opening sentence mostly with negative comments and rejections toward the participant, which should induce social-evaluative threat. Physiological and subjective responses were measured, before, during, and after the VISTTA in 61 (29 male and 32 female) first year students who received either mostly negative (<i>n</i> = 31; threat group) or neutral comments (<i>n</i> = 30; control group). Two-level behavioral validation included social threat-induced mood changes in participants, and interlocutor evaluation. The latter consisted of multiple variables such as "willingness to cooperate" after every conversation, an overall fairness evaluation of interlocutors, and evaluations per reaction indicating how positive or negative it was received. We acquired additional physiological measures including cortisol assays via saliva samples, heart rate, and blood pressure. Confirming our hypotheses, peer rejection and exclusion during the VISTTA led to less willingness to cooperate and lower fairness evaluation of interlocutors. It also induced feelings of anger and surprise and lower happiness in the social-threat group. Women showed overall higher emotion ratings compared to men. Contrary to our <i>a priori</i> hypothesis, the VISTTA did not induce cortisol and heart rate increases. However, the stable cortisol response in women in the threat group does not follow the circadian decline and might reflect an endocrinological response. The decline in cortisol response in men in both the threat and control group could indicate faster habituation to the VISTTA. Taken together, these findings indicate effects of social-evaluative threat on a behavioral level, and more moderate effects on the emotional and physiological level. Sex differences in affective and cortisol responses may indicate that women are more susceptible for the social-evaluative threat than men. With a realistic implementation of verbal, interactive, and social components, the VISTTA is designed as an fMRI paradigm that can be applied to elucidate the neural representation of social-evaluative threat.
In Vitro Immunodetection of Prothymosin Alpha in Normal and Pathological Conditions
2019-08-07

Prothymosin alpha (ProT&amp;#945;) is a highly acidic polypeptide, ubiquitously expressed in almost all mammalian cells and tissues and consisting of 109 amino acids in humans. ProT&amp;#945; is known to act both, intracellularly, as an anti-apoptotic and proliferation mediator, and extracellularly, as a biologic response modifier mediating immune responses similar to molecules termed as “alarmins”. Antibodies and immunochemical techniques for ProT&amp;#945; have played a leading role in the investigation of the biological role of ProT&amp;#945;, several aspects of which still remain unknown and contributed to unraveling the diagnostic and therapeutic potential of the polypeptide. This review deals with the so far reported antibodies along with the related immunodetection methodology for ProT&amp;#945; (immunoassays as well as immunohistochemical, immunocytological, immunoblotting, and immunoprecipitation techniques) and its application to biological samples of interest (tissue extracts and sections, cells, cell lysates and cell culture supernatants, body fluids), in health and disease states. In this context, literature information is critically discussed, and some concluding remarks are presented.
The Phenotypic Variation of a Parkin‐Related Parkinson's Disease Family and the Role of Heterozygosity
2019-08-07

ABSTRACT Background Parkinson's disease (PD) is a common neurodegenerative disorder with both sporadic occurrence and Mendelian heredity, as it is true for autosomal recessive parkin ‐related PD (PARK‐ parkin ). Parkin‐ related PD is characterized by early onset, slow progression, frequent lower limb dystonia, and a robust response to levodopa. Clinicians are increasingly confronted with heterozygous PD patients mimicking dominant inheritance. Nevertheless, the exact clinical implications of heterozygosity are not fully understood. Cases We present an illustrative PARK‐ parkin family with 2 affected sisters (compound heterozygous) and their father (heterozygous). One sister expresses the classical phenotype, whereas the other has isolated jerky tremor. The father has left‐sided action tremor of the hand with some dystonic posturing without clear bradykinesia and normal DaTSCAN. Conclusion This case series illustrates the phenotypic variability in parkin ‐related PD with 1 classical phenotype and 1 patient with isolated jerky tremor. Unilateral hand tremor of the heterozygous father could mislead genetic testing by mimicking dominant inheritance.
The global decline of freshwater megafauna
2019-08-08

Abstract Freshwater ecosystems are among the most diverse and dynamic ecosystems on Earth. At the same time, they are among the most threatened ecosystems but remain underrepresented in biodiversity research and conservation efforts. The rate of decline of vertebrate populations is much higher in freshwaters than in terrestrial or marine realms. Freshwater megafauna (i.e., freshwater animals that can reach a body mass ≥30 kg) are intrinsically prone to extinction due to their large body size, complex habitat requirements and slow life‐history strategies such as long life span and late maturity. However, population trends and distribution changes of freshwater megafauna, at continental or global scales, remain unclear. In the present study, we compiled population data of 126 freshwater megafauna species globally from the Living Planet Database and available literature, and distribution data of 44 species inhabiting Europe and the United States from literature and databases of the International Union for Conservation of Nature and NatureServe. We quantified changes in population abundance and distribution range of freshwater megafauna species. Globally, freshwater megafauna populations declined by 88% from 1970 to 2012, with the highest declines in the Indomalaya and Palearctic realms (−99% and −97%, respectively). Among taxonomic groups, mega‐fishes exhibited the greatest global decline (−94%). In addition, freshwater megafauna experienced major range contractions. For example, distribution ranges of 42% of all freshwater megafauna species in Europe contracted by more than 40% of historical areas. We highlight the various sources of uncertainty in tracking changes in populations and distributions of freshwater megafauna, such as the lack of monitoring data and taxonomic and spatial biases. The detected trends emphasize the critical plight of freshwater megafauna globally and highlight the broader need for concerted, targeted and timely conservation of freshwater biodiversity.
Untargeted Metabolomics Reveals Molecular Effects of Ketogenic Diet on Healthy and Tumor Xenograft Mouse Models
2019-08-08

The application of ketogenic diet (KD) (high fat/low carbohydrate/adequate protein) as an auxiliary cancer therapy is a field of growing attention. KD provides sufficient energy supply for healthy cells, while possibly impairing energy production in highly glycolytic tumor cells. Moreover, KD regulates insulin and tumor related growth factors (like insulin growth factor-1, IGF-1). In order to provide molecular evidence for the proposed additional inhibition of tumor growth when combining chemotherapy with KD, we applied untargeted quantitative metabolome analysis on a spontaneous breast cancer xenograft mouse model, using MDA-MB-468 cells. Healthy mice and mice bearing breast cancer xenografts and receiving cyclophosphamide chemotherapy were compared after treatment with control diet and KD. Metabolomic profiling was performed on plasma samples, applying high-performance liquid chromatography coupled to tandem mass spectrometry. Statistical analysis revealed metabolic fingerprints comprising numerous significantly regulated features in the group of mice bearing breast cancer. This fingerprint disappeared after treatment with KD, resulting in recovery to the metabolic status observed in healthy mice receiving control diet. Moreover, amino acid metabolism as well as fatty acid transport were found to be affected by both the tumor and the applied KD. Our results provide clear evidence of a significant molecular effect of adjuvant KD in the context of tumor growth inhibition and suggest additional mechanisms of tumor suppression beyond the proposed constrain in energy supply of tumor cells.
Auxiliary living donor liver transplantation combined with two-stage hepatectomy for unresectable colorectal liver metastases
2019-08-08

Purpose of review To review the role of liver transplantation for unresectable colorectal liver metastases (u-CRLM) and to describe the intial experience with auxiliary living donor liver transplantation combined with two-stage hepatectomy for u-CRLM (i.e. living donor RAPID). Recent findings Patients affected with u-CRLM have a poor prognosis with 5 years overall survival (OS) rate less than 10% under standard modern chemotherapy.There is an actual international consensus that liver transplantation for u-CRLM represents a viable option in highly selected patients with OS rate at 5 years up to 80% notwithstanding high recurrence rates. Due to the scarcity of whole liver graft from deceased donors, the RAPID procedure (i.e. resection and partial liver segment 2–3 transplantation from deceased donors with delayed total hepatectomy) has been introduced as possible alternative. The RAPID procedure represents the most actual and modern fusion of the two most challenging procedures of modern hepatobiliary and liver transplant surgery: that is auxiliary partial orthotopic liver transplantation and associating liver partition and portal vein ligation for staged hepatectomy. Although the deceased donor-RAPID procedure may show promising results, the basic problem of scarcity of organs from deceased donors and mainly the lack of splittable organs still remains. Summary The living donor RAPID, based on transplantation of left lateral segments from living donor, may represent the way out to this problem. It is feasible and safe (for both donor and recipient), but characterized by a very challenging high-end transplantological procedure.
Molecular Evolution of Extensively Drug-Resistant (XDR) Pseudomonas aeruginosa Strains From Patients and Hospital Environment in a Prolonged Outbreak
2019-08-08

In this study, we aimed to elucidate a prolonged outbreak of extensively drug-resistant (XDR) <i>Pseudomonas aeruginosa</i>, at two adjacent hospitals over a time course of 4 years. Since all strains exhibited a similar antibiotic susceptibility pattern and carried the carbapenemase gene bla<sub>VIM</sub>, a monoclonal outbreak was assumed. To shed light on the intra-hospital evolution of these strains over time, whole genome sequence (WGS) analysis of 100 clinical and environmental outbreak strains was employed. Phylogenetic analysis of the core genome revealed the outbreak to be polyclonal, rather than monoclonal as initially suggested. The vast majority of strains fell into one of two major clusters, composed of 27 and 59 strains, and their accessory genome each revealed over 400 and 600 accessory genes, respectively, thus indicating an unexpectedly high structural diversity among phylogenetically clustered strains. Further analyses focused on the cluster with 59 strains, representing the hospital from which both clinical and environmental strains were available. Our investigation clearly shows both accumulation and loss of genes occur very frequently over time, as reflected by analysis of protein enrichment as well as functional enrichment. In addition, we investigated adaptation through single nucleotide polymorphisms (SNPs). Among the genes affected by SNPs, there are a multidrug efflux pump (<i>mexZ</i>) and a mercury detoxification operon (<i>merR</i>) with deleterious mutations, potentially leading to loss of repression with resistance against antibiotics and disinfectants. Our results not only confirm WGS to be a powerful tool for epidemiologic analyses, but also provide insights into molecular evolution during an XDR <i>P. aeruginosa</i> hospital outbreak. Genome mutation unveiled a striking genetic plasticity on an unexpectedly high level, mostly driven by horizontal gene transfer. Our study adds valuable information to the molecular understanding of "real-world" Intra-hospital <i>P. aeruginosa</i> evolution and is a step forward toward more personalized medicine in infection control.
Phosphorus Pool Composition in Soils and Sediments of Transitional Ecotones under the Influence of Agriculture
2019-08-08

Transitional ecotones such as vegetated buffer strips, stream banks, and streambeds retain phosphorus (P) in the immediate surroundings of farmland. Yet the fate of P in these ecotones remains unclear. Our objectives were to (i) test the difference in the P pool composition of soil and sediment between sites surrounded by agriculture and forestry and (ii) test whether specific P pools differ among transitional ecotones. Phosphorus pools (by a modified Hedley fractionation scheme) and the degree of P saturation (DPS) were determined in 33 soil and sediment samples from eight farmland and three forest sites. At farmland sites, total P in soil and sediment was more than twofold higher as compared to forestry sites. The proportion of labile inorganic P (Pi) and the DPS were significantly larger in transitional ecotones close to farmland. We further used normalized values for comparing the respective ecotones at the sites. The deviation of each transitional ecotone relative to the respective site average revealed that the normalized total P concentration and proportions of labile and moderately labile Pi were significantly smaller in bed sediment adjacent to farmland as compared to respective stream bank and buffer strip soil, whereas the stable Ca‐Pi proportion was larger. The results reflected a decreased Pi sorption capacity in combination with Pi desorption and transfer of Pi into secondary Ca‐Pi minerals in bed sediment. In summary, the influence of agriculture increases labile P pools in soil and sediment, which are then subject to a succession of dynamic processes resulting in a partial loss of Pi to the aqueous phase as well as fixation of Pi in the Ca‐Pi pool. Core Ideas Agriculture has a pronounced impact on P status and pools in transitional ecotones. Labile Pi and degree of P saturation significantly increased in buffer strips, stream banks, and beds. We found more Ca‐Pi but less labile P in streambeds than in stream banks and buffer strips. There is presumable transfer of labile P into the Ca‐Pi pool but also in the dissolved phase. Further knowledge of P pool changes is needed to manage legacy P in catchments.
Decoding subcategories of human bodies from both body- and face-responsive cortical regions
2019-08-08

Our visual system can easily categorize objects (e.g. faces vs. bodies) and further differentiate them into subcategories (e.g. male vs. female). This ability is particularly important for objects of social significance, such as human faces and bodies. While many studies have demonstrated category selectivity to faces and bodies in the brain, how subcategories of faces and bodies are represented remains unclear. Here, we investigated how the brain encodes two prominent subcategories shared by both faces and bodies, sex and weight, and whether neural responses to these subcategories rely on low-level visual, high-level visual or semantic similarity. We recorded brain activity with fMRI while participants viewed faces and bodies that varied in sex, weight, and image size. The results showed that the sex of bodies can be decoded from both body- and face-responsive brain areas, with the former exhibiting more consistent size-invariant decoding than the latter. Body weight could also be decoded in face-responsive areas and in distributed body-responsive areas, and this decoding was also invariant to image size. The weight of faces could be decoded from the fusiform body area (FBA), and weight could be decoded across face and body stimuli in the extrastriate body area (EBA) and a distributed body-responsive area. The sex of well-controlled faces (e.g. excluding hairstyles) could not be decoded from face- or body-responsive regions. These results demonstrate that both face- and body-responsive brain regions encode information that can distinguish the sex and weight of bodies. Moreover, the neural patterns corresponding to sex and weight were invariant to image size and could sometimes generalize across face and body stimuli, suggesting that such subcategorical information is encoded with a high-level visual or semantic code.
Stability of the co-orbital resonance under dissipation
2019-08-08

Despite the existence of co-orbital bodies in the solar system, and the prediction of the formation of co-orbital planets by planetary system formation models, no co-orbital exoplanets (also called trojans) have been detected thus far. In this paper we investigate how a pair of co-orbital exoplanets would fare during their migration in a protoplanetary disc. To this end, we computed a stability criterion of the Lagrangian equilibria L 4 and L 5 under generic dissipation and slow mass evolution. Depending on the strength and shape of these perturbations, the system can either evolve towards the Lagrangian equilibrium, or tend to increase its amplitude of libration, possibly all the way to horseshoe orbits or even exiting the resonance. We estimated the various terms of our criterion using a set of hydrodynamical simulations, and show that the dynamical coupling between the disc perturbations and both planets have a significant impact on the stability: the structures induced by each planet in the disc perturb the dissipative forces applied on the other planets over each libration cycle. Amongst our results on the stability of co-orbitals, several are of interest to constrain the observability of such configurations: long-distance inward migration and smaller leading planets tend to increase the libration amplitude around the Lagrangian equilibria, while leading massive planets and belonging to a resonant chain tend to stabilise it. We also show that, depending on the strength of the dissipative forces, both the inclination and the eccentricity of the smaller of the two co-orbitals can be significantly increased during the inward migration of the co-orbital pair, which can have a significant impact on the detectability by transit of such configurations.
The impact of language contact on the Quechua varieties of Northern Peru
2019-08-08

Abstract Studies of language contact in the Central Andes of Peru and Bolivia have focused strongly on the present-day contact situation between Quechua and Spanish, and the intricate and multilayered contact relationship between the Quechua and Aymara lineages. There are fewer studies of the influence of Quechua on minor non-Quechua languages of the Andes, and still fewer studies which, conversely, explore the influence of non-Quechua languages on Quechua. Focusing on the lexicon, this article explores the impact of the complex linguistic ecology of Northern Peru on the five Quechua varieties of that region—Lambayeque, Cajamarca, Chachapoyas, San Martín and Ancash Quechua. The study identifies lexical items that lack clear Quechua etymologies in the relevant varieties and carries out external comparisons of these items with the vocabulary of the non-Quechua languages of Northern Peru to identify possible sources. Results show that borrowing is mostly localized: that is, whereas influence from Amazonian lowland languages is almost exclusively found in the eastern varieties of Chachapoyas and San Martín, highland Quechua varieties have typically borrowed from neighboring highland languages.