julilili42/papers huggingface dataset:
Regulatory phosphorylation event of phosphoglucomutase 1 tunes its activity to regulate glycogen metabolism
2022-05-05

Regulation of glycogen metabolism is of vital importance in organisms of all three kingdoms of life. Although the pathways involved in glycogen synthesis and degradation are well known, many regulatory aspects around the metabolism of this polysaccharide remain undeciphered. Here, we used the unicellular cyanobacterium Synechocystis as a model to investigate how glycogen metabolism is regulated in nitrogen‐starved dormant cells, which entirely rely on glycogen catabolism to resume growth upon nitrogen repletion. We identified phosphoglucomutase 1 (PGM1) as a key regulatory point in glycogen metabolism, and post‐translational modification as an essential mechanism for controlling its activity. We could show that PGM1 is phosphorylated ata residue in the regulatory latch domain (Ser 47) during nitrogen starvation, which inhibits its activity. Inactivation of PGM1 by phosphorylation at Ser 47 prevents premature degradation of the glycogen stores and appears to be essential for survival of Synechocystis in the dormant state. Remarkably, this regulatory mechanism seems to be evolutionary conserved in PGM1 enzymes, from bacteria to humans.
Os acromiale: prevalence and associated patient-related factors—a population-based study of three thousand and fifty participants
2022-05-05

Abstract Purpose The presence of os acromiale is of clinical relevance before performing shoulder surgery but ethnic differences and little information regarding associated factors seem to be present. Population-based studies to clarify these topics are essential so the purpose of this study was to assess the prevalence, anatomy, and associations of os acromiale in a general adult population. Methods Both shoulders of 3050 participants from the population-based Study of Health in Pomerania (SHIP) were assessed on magnetic resonance imaging (MRI). Associations with the os acromiale were calculated for sex, age, body height, body weight, and heavy mechanical oscillations on the upper extremity. Results In total, 1.9% (58/3050) had an os acromiale, while 21 were unilateral left, 23 were unilateral right, and 14 were bilateral. Sixty-eight meso-acromions, three pre-acromions, and one meta-acromion were detected. Os acromiale were more frequent in men (right side: p = 0.037, left side: p = 0.005). Overall, no differences in sides ( p = 0.808), to participants’ age (right: p = 0.993, left: p = 0.499), body height (right side: p = 0.241, left side: p = 0.154), and the exposure to heavy mechanical oscillations on the upper extremity (right: p = 0.054, left: p = 0.117) were detected. Conclusion Our results support the genetic theory for the aetiology of the os acromiale due to the lower prevalence of the os acromiale in north-eastern Germany compared to the worldwide prevalence (1.9 to 7%) and the lacking association to lifestyle, age, gender, or sides. Additionally, it is important to be aware of possible os acromiale before surgery.
Mycothiol Peroxidase Activity as a Part of the Self-Resistance Mechanisms against the Antitumor Antibiotic Cosmomycin D
2022-05-05

Anthracycline compounds are DNA intercalating agents widely used in cancer chemotherapeutic protocols. This work focused on the self-resistance mechanisms developed by the cosmomycin-producing bacterium Streptomyces olindensis .
Logarithmic negativity in out-of-equilibrium open free-fermion chains: An exactly solvable case
2022-05-05

We derive the quasiparticle picture for the fermionic logarithmic negativity in a tight-binding chain subject to gain and loss dissipation. We focus on the dynamics after the quantum quench from the fermionic Néel state. We consider the negativity between both adjacent and disjoint intervals embedded in an infinite chain. Our result holds in the standard hydrodynamic limit of large subsystems and long times, with their ratio fixed. Additionally, we consider the weakly-dissipative limit, in which the dissipation rates are inversely proportional to the size of the intervals. We show that the negativity is proportional to the number of entangled pairs of quasiparticles that are shared between the two intervals, as is the case for the mutual information. Crucially, in contrast with the unitary case, the negativity content of quasiparticles is not given by the Rényi entropy with Rényi index 1/2, and it is in general not easily related to thermodynamic quantities.
Di-(2-ethylhexyl) phthalate substitutes accelerate human adipogenesis through PPARγ activation and cause oxidative stress and impaired metabolic homeostasis in mature adipocytes
2022-05-06

The obesity pandemic is presumed to be accelerated by endocrine disruptors such as phthalate-plasticizers, which interfere with adipose tissue function. With the restriction of the plasticizer di-(2-ethylhexyl)-phthalate (DEHP), the search for safe substitutes gained importance. Focusing on the master regulator of adipogenesis and adipose tissue functionality, the peroxisome proliferator-activated receptor gamma (PPARγ), we evaluated 20 alternative plasticizers as well as their metabolites for binding to and activation of PPARγ and assessed effects on adipocyte lipid accumulation. Among several compounds that showed interaction with PPARγ, the metabolites MINCH, MHINP, and OH-MPHP of the plasticizers DINCH, DINP, and DPHP exerted the highest adipogenic potential in human adipocytes. These metabolites and their parent plasticizers were further analyzed in human preadipocytes and mature adipocytes using cellular assays and global proteomics. In preadipocytes, the plasticizer metabolites significantly increased lipid accumulation, enhanced leptin and adipsin secretion, and upregulated adipogenesis-associated markers and pathways, in a similar pattern to the PPARγ agonist rosiglitazone. Proteomics of mature adipocytes revealed that both, the plasticizers and their metabolites, induced oxidative stress, disturbed lipid storage, impaired metabolic homeostasis, and led to proinflammatory and insulin resistance promoting adipokine secretion. In conclusion, the plasticizer metabolites enhanced preadipocyte differentiation, at least partly mediated by PPARγ activation and, together with their parent plasticizers, affected the functionality of mature adipocytes similar to reported effects of a high-fat diet. This highlights the need to further investigate the currently used plasticizer alternatives for potential associations with obesity and the metabolic syndrome.
Role of Heterojunctions of Core–Shell Heterostructures in Gas Sensing
2022-05-06

Heterostructures made from metal oxide semiconductors (MOS) are fundamental for the development of high-performance gas sensors. Since their importance in real applications, a thorough understanding of the transduction mechanism is vital, whether it is related to a heterojunction or simply to the shell and core materials. A better understanding of the sensing response of heterostructured nanomaterials requires the engineering of heterojunctions with well-defined core and shell layers. Here, we introduce a series of prototypes CNT-<sub>n</sub>MOS, CNT-<sub>p</sub>MOS, CNT-<sub>p</sub>MOS-<sub><i>n</i></sub>MOS, and CNT-<sub><i>n</i></sub>MOS-<sub>p</sub>MOS hierarchical core-shell heterostructures (CSHS) permitting us to directly relate the sensing response to the MOS shell or to the p-n heterojunction. The carbon nanotubes are here used as highly conductive substrates permitting operation of the devices at relatively low temperature and are not involved in the sensing response. NiO and SnO<sub>2</sub> are selected as representative p- and n-type MOS, respectively, and the response of a set of samples is studied toward hydrogen considered as model analyte. The CNT-<sub>n,p</sub>MOS CSHS exhibit response related to the <sub>n,p</sub>MOS-shell layer. On the other hand, the CNT-<sub>p</sub>MOS-<sub>n</sub>MOS and CNT-<sub>n</sub>MOS-<sub>p</sub>MOS CSHS show sensing responses, which in certain cases are governed by the heterojunctions between <sub>n</sub>MOS and <sub>p</sub>MOS and strongly depends on the thickness of the MOS layers. Due to the fundamental nature of this study, these findings are important for the development of next generation gas sensing devices.
Polarimetric radar reveals the spatial distribution of ice fabric at domes and divides in East Antarctica
2022-05-06

Abstract. Ice crystals are mechanically and dielectrically anisotropic. They progressively align under cumulative deformation, forming an ice-crystal-orientation fabric that, in turn, impacts ice deformation. However, almost all the observations of ice fabric are from ice core analysis, and its influence on the ice flow is unclear. Here, we present a non-linear inverse approach to process co- and cross-polarized phase-sensitive radar data. We estimate the continuous depth profile of georeferenced ice fabric orientation along with the reflection ratio and horizontal anisotropy of the ice column. Our method approximates the complete second-order orientation tensor and all the ice fabric eigenvalues. As a result, we infer the vertical ice fabric anisotropy, which is an essential factor to better understand ice deformation using anisotropic ice flow models. The approach is validated at two Antarctic ice core sites (EPICA (European Project for Ice Coring in Antarctica) Dome C and EPICA Dronning Maud Land) in contrasting flow regimes. Spatial variability in ice fabric characteristics in the dome-to-flank transition near Dome C is quantified with 20 more sites located along with a 36 km long cross-section. Local horizontal anisotropy increases under the dome summit and decreases away from the dome summit. We suggest that this is a consequence of the non-linear rheology of ice, also known as the Raymond effect. On larger spatial scales, horizontal anisotropy increases with increasing distance from the dome. At most of the sites, the main driver of ice fabric evolution is vertical compression, yet our data show that the horizontal distribution of the ice fabric is consistent with the present horizontal flow. This method uses polarimetric-radar data, which are suitable for profiling radar applications and are able to constrain ice fabric distribution on a spatial scale comparable to ice flow observations and models.
Manipulating Picosecond Photoresponse in van der Waals Heterostructure Photodetectors
2022-05-06

Abstract Self‐powered ultrafast 2D photodetectors have demonstrated great potential in imaging, sensing, and communication. Understanding the intrinsic ultrafast charge carrier generation and separation processes is essential for achieving high‐performance devices. However, probing and manipulating the ultrafast photoresponse is limited either by the temporal resolution of the conventional methods or the required sophisticated device configurations. Here, van der Waals heterostructure photodetectors are constructed based on MoS 2 /WSe 2 p–n and n–n junctions and manipulate the picosecond photoresponse by combining photovoltaic (PV) and photothermoelectric (PTE) effects. Taking time‐resolved photocurrent (TRPC) measurements, a TRPC peak at zero time delay is observed with decay time down to 4 ps in the n–n junction device, in contrast to the TRPC dip in the p–n junction and pure WSe 2 devices, indicating an opposite current polarity between PV and PTE. More importantly, with an ultrafast photocurrent modulation, a transition from a TRPC peak to a TRPC dip is realized, and detailed carrier transport dynamics are analyzed. This study provides a deeper understanding of the ultrafast photocurrent generation mechanism in van der Waals heterostructures and offers a new perspective in instruction for designing more efficient self‐powered photodetectors.
Interplay between family background and labour-market conditions in shaping students’ occupational status expectations
2022-05-06

This study examines the relevance of labour-market conditions for individual occupational status expectations. We are particularly interested in students' status expectations in the final stages of their school careers. Occupational expectations are an important basis for adolescents' biographical decisions and corresponding transitions to vocational training and employment. By anticipating their likely labour-market situation, adolescents adjust their occupational choices; however, this anticipation is strongly moderated by their family background. We demonstrate this interaction using the example of school students in Germany's secondary education's tracked system. We link survey data from the German National Educational Panel Study to regionalised administrative data on unemployment. We find expectations for higher-status occupations in poor regional labour markets among students in non-academic school tracks. In contrast, students in the academic track aspire to lower status occupations in poor regional labour markets. In both cases, higher parental occupational status mitigates the impact of labour-market conditions on individual occupational status expectations. Our results indicate a greater awareness of structural conditions in the region among adolescents who are equipped with fewer familial resources.
Performance Differences in Male Youth Basketball Players According to Selection Status and Playing Position: An Evaluation of the Basketball Learning and Performance Assessment Instrument
2022-05-06

The Basketball Learning and Performance Assessment Instrument (BALPAI) has been initially developed and evaluated to assess the performance of students or youth basketball players on the entry level. As it is currently the only observational instrument that allows an overall assessment of players’ in-game performance, it might represent a valuable tool for talent identification and development purposes. To investigate this potential field of application, this study aimed to evaluate the BALPAI regarding reliability and diagnostic validity when assessing youth basketball players within a competitive setting. The study sample comprised N = 54 male youth players ( M age = 14.36 ± 0.33 years) of five regional selection teams (Point Guards, PG: n = 19; Shooting Guards and Small Forwards, SG/SF: n = 21; and Power Forwards and Centers, PF/C: n = 14) that competed at the annual U15 national selection tournament of the German Basketball Federation ( n = 24 selected; n = 30 non-selected). A total of 1997 ball-bound actions from five games were evaluated with BALPAI. The inter-rater reliability was assessed for technical execution, decision making, and final efficacy. The diagnostic validity of the instrument was examined via mean group comparisons of the players’ offensive game involvement and performance regarding both selection-dependent and position-dependent differences. The inter-rater reliability was confirmed for all performance-related components ( κ adj ≥ 0.51) while diagnostic validity was established only for specific the BALPAI variables. The selection-dependent analysis demonstrated higher offensive game involvement of selected players in all categories ( p &amp;lt; 0.05, 0.27 ≤ Φ ≤ 0.40) as well as better performance in shooting and receiving ( p &amp;lt; 0.05, 0.23 ≤ Φ ≤ 0.24). Within the positional groups, the strongest effects were demonstrated among PG ( p &amp;lt; 0.05, 0.46 ≤ Φ ≤ 0.60). The position-dependent analysis revealed that PG are more involved in total ball-bound actions ( p &amp;lt; 0.05; 0.34 ≤ Φ ≤ 0.53), passing ( p &amp;lt; 0.001; 0.55 ≤ Φ ≤ 0.67), and dribbling ( p &amp;lt; 0.05, 0.45 ≤ Φ ≤ 0.69) compared to players in other positions. Further differences between players according to selection status and playing position were not detected. The results of this evaluation indicate that the instrument, in its current form, is not yet applicable in competitive youth basketball. The findings highlight the importance of optimizing BALPAI for reliable and valid performance assessments in this context. Future studies should investigate the application of stricter and position-specific criteria to use the observational tool for talent identification and development purposes.
Optimal control for a coupled spin-polarized current and magnetization system
2022-05-06

Abstract This paper is devoted to an optimal control problem of a coupled spin drift-diffusion Landau–Lifshitz–Gilbert system describing the interplay of magnetization and spin accumulation in magnetic-nonmagnetic multilayer structures, where the control is given by the electric current density. A variational approach is used to prove the existence of an optimal control. The first-order necessary optimality system for the optimal solution is derived in one space-dimension via Lagrange multiplier method. Numerical examples are reported to validate the theoretical findings.
Major Allergen Content in Allergen Immunotherapy Products: The Limited Value of Numbers
2022-05-06

The prevalence of allergic disorders has increased drastically over the last 50 years to the extent that they can be considered epidemic. At present, allergen-specific immunotherapy (AIT) is the only therapy that targets the underlying cause of allergic disorders, and evidence of its superiority is based on data accumulated from clinical trials and observational studies demonstrating efficacy and safety. However, several aspects remain unresolved, such as harmonization and standardization of manufacturing and quantification procedures across manufacturers, homogeneous reporting of strength, and the establishment of international reference standards for many allergens. This article discusses issues related to the measurement of major allergen content in AIT extracts, raising the question of whether comparison of products from different manufacturers is an appropriate basis for selecting a specific AIT product. Allergen standardization in immunotherapy products is critical for ensuring quality and, thereby, safety and efficacy. However, lack of harmonization in manufacturing processes, allergen quantification (methodologies and references), national regulatory differences, clinical practice, and labeling shows that the comparison of AIT products based solely on major allergen amounts is not rational and, in fact, impossible. Moreover, when rating the information given for a specific product, it is necessary to take into account further inherent characteristics of products and their application in clinical practice, such as the state of extract modification, addition of adjuvant or adjuvant system, route of administration (sublingual/ subcutaneous), and cumulative dose as per posology (including the volume per administration). Finally, only convincing clinical data can serve as the basis for product-specific evaluation and cross-product comparability of individual products.
Enhanced expression of p21 promotes sensitivity of melanoma cells towards targeted therapies
2022-05-06

Metastatic melanoma patients benefit from the approved targeted BRAF inhibitor (BRAFi) therapy. Despite the great progress in the therapeutic approach to combat metastatic melanoma, fast emerging drug resistance in patients limits its long-term efficacy. In this study, we aimed to unravel the role of the p53 target gene CDKN1A/p21 in the response of melanoma cells towards BRAFi. We show that p53 activation increases BRAFi sensitivity in a synergistic manner exclusively in cells with a high expression of CDKN1A/p21. In a similar way, high expression of p21 was associated with a better response towards the mouse double minute 2 inhibitor (MDM2i) compared to those with low p21 expression. Indeed, p21 knockdown decreased the sensitivity towards both targeted therapies. The results indicate that the sensitivity of melanoma cells towards targeted therapies (BRAFi and MDM2i) is dependent on the p21 protein level in the cells. In addition to that, we found that p53 negatively regulates p73 expression; however, p73 seems not to have an influence on p53 expression. These findings offer new potential strategies for the treatment improvement of melanoma patients with high basal p21 levels with BRAFi by increasing treatment efficacy using combination therapies with p53 activating substances, which are able to further increase p21 expression levels. Furthermore, the data suggest that the expression and induction level of p21 could be used as a predictive biomarker in melanoma patients to forecast the outcome of a treatment with p53 activating substances and BRAFi. All in all, this manuscript shows the distinct role of p53 family members and its impact on melanoma therapy. In future, individualized treatment regimens based on p21 basal and induction levels could help melanoma patients with limited treatment options.
Role of ABO Blood Group in SARS-CoV-2 Infection in Households
2022-05-06

An association between certain ABO/Rh blood groups and susceptibility to SARS-CoV-2 infection has been proposed for adults, although this remains controversial. In children and adolescents, the relationship is unclear due to a lack of robust data. Here, we investigated the association of ABO/Rh blood groups and SARS-CoV-2 in a multi-center study comprising 163 households with 281 children and 355 adults and at least one SARS-CoV-2 seropositive individual as determined by three independent assays as a proxy for previous infection. In line with previous findings, we found a higher frequency of blood group A (+ 6%) and a lower frequency of blood group O (−6%) among the SARS-CoV-2 seropositive adults compared to the seronegative ones. This trend was not seen in children. In contrast, SARS-CoV-2 seropositive children had a significantly lower frequency of Rh-positive blood groups. ABO compatibility did not seem to play a role in SARS-CoV-2 transmission within the families. A correction for family clusters was performed and estimated fixed effects of the blood group on the risk of SARS-CoV-2 seropositivity and symptomatic infection were determined. Although we found a different distribution of blood groups in seropositive individuals compared to the reference population, the risk of SARS-CoV-2 seropositivity or symptomatic infection was not increased in children or in adults with blood group A or AB versus O or B. Increasing age was the only parameter positively correlating with the risk of SARS-CoV-2 infection. In conclusion, specific ABO/Rh blood groups and ABO compatibility appear not to predispose for SARS-CoV-2 susceptibility in children.