2058 POTENTIAL OF ODORANT RECEPTORS IN PROSTATE CANCER IMAGING
2010-03-23

You have accessJournal of UrologyProstate Cancer: Markers I1 Apr 20102058 POTENTIAL OF ODORANT RECEPTORS IN PROSTATE CANCER IMAGING Alexander Sturzu, Christian Schwentner, Joerg Hennenlotter, Hartmut Echner, Arnulf Stenzl, and Stefan Heckl Alexander SturzuAlexander Sturzu , Christian SchwentnerChristian Schwentner , Joerg HennenlotterJoerg Hennenlotter , Hartmut EchnerHartmut Echner , Arnulf StenzlArnulf Stenzl , and Stefan HecklStefan Heckl View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2105AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Imaging of prostate cancer is very unsatisfactory. Therefore, early diagnosis of prostate cancer and the detection of metastases still remains a great challenge. Recently, olfactory receptors have been detected in healthy and malignant prostate tissue. If conjugated to a marker-substance, olfactory receptor ligands would represent candidates for markers of not only olfactory cells but also prostate tissue. Such a conjugate could be of great value for the early detection of prostate cancer metastases. METHODS We coupled sulforhodamine, a fluorescence dye, to undecylic aldehyde, the antagonist of the lily-of-the-valley fragrance receptor. By using confocal laser scanning microscopy and flow cytometry we examined the uptake of this conjugate into various different human cell lines. One healthy prostate cell line (CC2555), two different prostate carcinoma cell lines (LNCaP, PC3), and five other carcinoma cell lines (Colo 205, U 373, U2OS, HeLa, Jurkat) were investigated. CD1 nude mice bearing human PC3 prostate carcinoma xenografts were injected with the conjugate. Rhodamine fluorescence of mouse organ frozen sections was evaluated by confocal laser scanning microscopy. RESULTS When comparing the seven human cell lines, the conjugate was preferentially taken up by the cytoplasm of healthy and malignant prostate cells. The mice showed high conjugate uptake into the xenografts, but much lower uptake into the mouse organs. After 3 hr of circulation, efflux could be observed in the xenograft sections. Xenograft touch prints confirmed in vivo intracellular accumulation. CONCLUSIONS These observations indicate that undecyclic acid, the antagonist of lily-of-the-valley fragrance receptor has potential for the development of prostate cancer-specific imaging agents. These may be used for the detection of both, organ-confined as well as metatstatic prostate cancer. This conjugate may be therefore valuable in the diagnosis of prostate cancer and its metastases. Tuebingen, Germany© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e800 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.Metrics Author Information Alexander Sturzu More articles by this author Christian Schwentner More articles by this author Joerg Hennenlotter More articles by this author Hartmut Echner More articles by this author Arnulf Stenzl More articles by this author Stefan Heckl More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...