julilili42/papers huggingface dataset:
Mobility and growth in confined spaces are important mechanisms for the establishment of Bacillus subtilis in the rhizosphere
2024-08-06

The rhizosphere hosts complex and abundant microbiomes whose structure and composition are now well described by metagenomic studies. However, the dynamic mechanisms that enable micro-organisms to establish along a growing plant root are poorly characterized. Here, we studied how a motile bacterium utilizes the microhabitats created by soil pore space to establish in the proximity of plant roots. We have established a model system consisting of Bacillus subtilis and lettuce seedlings co-inoculated in transparent soil microcosms. We carried out live imaging experiments and developed image analysis pipelines to quantify the abundance of the bacterium as a function of time and position in the pore space. Results showed that the establishment of the bacterium in the rhizosphere follows a precise sequence of events where small islands of mobile bacteria were first seen forming near the root tip within the first 12–24 h of inoculation. Biofilm was then seen forming on the root epidermis at distances of about 700–1000 μm from the tip. Bacteria accumulated predominantly in confined pore spaces within 200 μm from the root or the surface of a particle. Using probabilistic models, we could map the complete sequence of events and propose a conceptual model of bacterial establishment in the pore space. This study therefore advances our understanding of the respective role of growth and mobility in the efficient colonization of bacteria in the rhizosphere.
Anatomical and electrophysiological analysis of the fasciola cinerea of the mouse hippocampus
2024-08-06

Abstract The hippocampus is considered essential for several forms of declarative memory, including spatial and social memory. Despite the extensive research of the classic subfields of the hippocampus, the fasciola cinerea (FC)—a medially located structure within the hippocampal formation—has remained largely unexplored. In the present study, we performed a morpho‐functional characterization of principal neurons in the mouse FC. Using in vivo juxtacellular recording of single neurons, we found that FC neurons are distinct from neighboring CA1 pyramidal cells, both morphologically and electrophysiologically. Specifically, FC neurons displayed non‐pyramidal morphology and granule cell‐like apical dendrites. Compared to neighboring CA1 pyramidal neurons, FC neurons exhibited more regular in vivo firing patterns and a lower tendency to fire spikes at short interspike intervals. Furthermore, tracing experiments revealed that the FC receives inputs from the lateral but not the medial entorhinal cortex and CA3, and it provides a major intra‐hippocampal projection to the septal CA2 and sparser inputs to the distal CA1. Overall, our results indicate that the FC is a morphologically and electrophysiologically distinct subfield of the hippocampal formation; given the established role of CA2 in social memory and seizure initiation, the unique efferent intra‐hippocampal connectivity of the FC points to possible roles in social cognition and temporal lobe epilepsy.
On the importance of diversity in ecological research
2024-08-06

Societal Impact Statement Protecting diversity is critical to maintaining functional and adaptive ecosystems. However, research groups that explore these relationships tend to lack diversity themselves. To address the challenges of our changing world, it is crucial to consider a wide range of perspectives and knowledge. We propose that just as greater diversity in ecosystems relates to a variety of functions and, consequently, enhances resilience, stability, and efficiency, a comparable diversity within research teams results in a broader spectrum of creative endeavors. Thus, it is important to re‐evaluate our scientific communities' openness toward greater diversity to meet the diverse problems of our changing world. Summary Ecosystems face multiple threats, jeopardizing natural and human well‐being. Biodiversity loss can increase ecosystem vulnerability to global change, risking abrupt and irreversible changes. Protecting biodiversity can prevent ecosystems from crossing tipping points, preserving their functions and resilience, which sustain life on our planet. In addressing these challenges, society demands creativity, innovation, and diverse perspectives. Yet, our societal structures often neglect the full range of voices and experiences, limiting problem‐solving. Understanding how research teams with diverse demographics may stimulate creative solutions to global change challenges may help. In this context, we emphasize examples from plant ecology, where the advantages of diverse ecosystems are well recognized, and we draw an analogy of these features to the innovation of research teams. In ecosystems, biodiversity can maximize productivity and function but can also serve as a protective “insurance” against disruptions. Similarly, in a time of multifaceted environmental changes, diverse research groups play a critical and analogous role, resembling the biodiversity within ecosystems. Diverse research teams not only bring complementary strengths, fostering creative problem‐solving by embracing various perspectives, but also drive scientific innovation by integrating a wide range of research topics (e.g., ecosystems, taxa, and conservation priorities). These human‐centered dynamics are congruent with the mechanisms in ecosystems, with a diversity of solutions promoting resilience, stability, and efficiency, all of which ultimately provide a counterbalancing effect on global change. Much like biodiverse ecosystems, a diverse spectrum of knowledge, and expertise within the scientific community can enrich a dynamic landscape of innovation.
Salt induced slowdown of kinetics and dynamics during thermal gelation of egg-yolk
2024-08-06

We investigated the effect of the NaCl concentration (0.3–2M) on the structure and dynamics of hen egg yolk at room temperature and during thermal gelation at temperatures in the range of 66–90 °C utilizing low-dose x-ray photon correlation spectroscopy in ultra-small angle x-ray scattering geometry. With an increase in the salt concentration, we observe progressive structural and dynamic changes at room temperature, indicating the disruption of yolk components such as yolk-granules and yolk-plasma proteins. Temperature- and salt-dependent structural and dynamic investigations suggest a delay in the gel formation and aggregation of yolk low-density lipoproteins with increasing ionic strength. However, the time–temperature superposition relationship observed in all samples suggests an identical mechanism underlying protein aggregation–gelation with a temperature-dependent reaction rate. The sol–gel transition time extracted from kinetic and dynamic information follows Arrhenius’s behavior, and the activation energy (460 kJ/mol) is found to be independent of the salt concentration.
From party to private property: Early battles over press ownership in post-wall Germany
2024-08-06

This article provides a historical case study analysis of a press system in transition and the role of ownership therein. More specifically, the article looks at early battles over press ownership fought by West German publishing houses in socialist East Germany after the fall of the Berlin Wall. By means of early joint-venture agreements and financial investments, these publishers entered alliances with newspapers long before the latter were officially privatized by the Treuhandanstalt—a semi-public institution responsible for privatizing the entire economy of the German Democratic Republic (GDR). To make this point, the article first sketches some relevant theoretical concepts, then introduces the GDR press system, and finally shows how pre-emptive joint-venture deals became the key to privatizing an entire press system. By uncovering the underlying dynamics of media in transition, this article adds a historical current to the understanding of more recent transformations in media and communications.
Surgical Outcomes in Octogenarians with Heart Failure and Reduced Ejection Fraction following Isolated Coronary Artery Bypass Grafting—A Propensity Score Matched Analysis
2024-08-06

Background/Objectives: The demographic shift towards an aging population necessitates a reevaluation of surgical interventions like coronary artery bypass grafting (CABG) in octogenarians. This study aims to elucidate the outcomes of CABG in octogenarians with heart failure and reduced ejection fraction (HFrEF), a group traditionally considered at high risk for such procedures. Methods: Conducted across four academic hospitals in Germany from 2017 to 2023, this retrospective multicenter study assessed 100 patients (50 octogenarians ≥80 years and 50 non-octogenarians &lt;80 years) with HFrEF undergoing isolated CABG. Through propensity score matching, the study aimed to compare the incidence of major adverse cardiac and cerebrovascular events (MACCEs), as well as other clinical endpoints, between the two groups. Statistical analyses included chi-square, ANOVA, Mann–Whitney U test, Cox regression, and logistic regression, aiming to identify significant differences in outcomes. Results: The study revealed no significant difference in the combined incidence of MACCEs between octogenarians and non-octogenarians (OR: 0.790, 95% CI: 0.174–3.576, p = 0.759). Mortality rates were similar across groups (7% each, p = 1.000), as were occurrences of postoperative myocardial infarction (2% each, p = 1.000) and stroke (3% total). Secondary outcomes like delirium (17% total, no significant age group difference, p = 0.755), acute kidney injury (18% total, p = 0.664), and the need for dialysis (14% total, p = 1.000) also showed no differences between age groups. Interestingly, non-octogenarians required more packed red blood cells during their stay (p = 0.008), while other postoperative care metrics, such as hospital and ICU length of stay and ventilation hours, were comparable across groups. Conclusion: This multicenter study highlights that CABG is a viable and safe surgical option for octogenarians with HFrEF, challenging prior assumptions about the elevated risks associated with performing this procedure in older patients. The absence of significant differences in the incidence of MACCEs and other postoperative complications across age groups emphasizes the importance of careful patient selection and perioperative management. These findings advocate for a more inclusive approach to surgical treatment for octogenarians with HFrEF, suggesting that age alone should not be a determinant for CABG eligibility. This study contributes critical insights into optimizing care for a high-risk demographic, indicating a need for tailored guidelines that accommodate the aging population with complex cardiac conditions.
African Socialism and Secular State Formation
2024-08-06

Abstract In order to trace pathways of secularisation and secularity in Africa this paper highlights a particular movement that carried great ideological weight at the time of most countries’ independence in the 1950s and 60s, namely African socialism. The development toward state secularism was structurally very similar throughout the continent independently of whether political leaders opted for the ‘West’ or the ‘East’ in the cold war. However, in opposition to Soviet ideology, African Socialism was famously antiatheist. With the wish to fend off Marxist atheism as a supposedly necessary aspect of socialism, ideologues in African socialism were among the few politicians in Africa even to address the place of religion in a secular state at all. The roots of African socialism can be traced to US-American Pan-Africanism as well as the interconnected colonial opposition movement grounded in Marxist anti-imperialism. Another argument focusses on the education of some prominent state leaders, such as Nyerere, Nkrumah, Touré and Senghor, to explain the importance of Christian mission schools and Islamic madrasahs as points of access to social, intellectual, and institutional participation in global anti-colonial movements. In the framework of one-party politics, state leaders called on (Pan-)African traditions, but ‘de-mystified’ them (Touré) in order to enhance African Socialism ‘as belief’ (Nyerere). In conclusion it is argued that state secularism in Africa at the time of independence, as demonstrated most visibly in African Socialism, is more about suppressing and/or balancing the traditional powers of religious leaders than about a fundamental critique of a religious way of life. In turn, the implicit association of socialism and Marxism with atheism needs further scrutiny in a global perspective.
Multiple Secularities in Africa – An Introduction
2024-08-06

Abstract Raising timely and urgent questions about the forms, scope and boundaries of religious authority and practice, this article offers novel ways for the study of secularities and secularisms in contemporary African societies. In recent scholarly debates on secularity, Africa has been marginal. Part of the reason, it was suggested was that African ways of being in, and knowing, the world lay outside the religious-secular divide. We contest such positions. Secularism was clearly part of modernists colonial ideologies that called for the eradication of African beliefs described as backward and irrational. We find that the colonial encounter had a powerful historical impact, essentializing and othering African societies as marked by holistic indigenous cultures rather than differentiated religions. We suggest that the complex interplay of different African and European cultures has simultaneously shaped the social construction and historical development of multiple secularities. We propose that the concept of multiple secularities provides creative avenues to rethink religion, political authority and belonging. We consider secularities as contested arrangements of religious and other spheres whose dynamics include processes of de-differentiation and de-secularization.
Deglaciation and abrupt events in a coupled comprehensive atmosphere–ocean–ice sheet–solid earth model
2024-08-07

Abstract. During the last 20,000 years the climate of the earth has changed from a state much colder than today with large ice sheets in North America and Northwest Eurasia to its present state. The fully-interactive simulation of this transition represents a hitherto unsolved challenge for state-of-the-art climate models. We use a novel coupled comprehensive atmosphere–ocean–vegetation– ice sheet–solid earth model to simulate the transient climate evolution from the last glacial maximum to preindustrial times. The model considers dynamical changes of the glacier mask, land–sea mask and river routing. An ensemble of transient model simulations successfully captures the main features of the last deglaciation, as depicted by proxy estimates. In addition, our model simulates a series of abrupt climate changes, which can be attributed to different drivers. Abrupt cooling events during the glacial and the first half of the deglaciation are caused by Heinrich-event like ice-sheet surges, which are part of the model generated internal variability. We show that the timing of these surges depends on the initial state and the model parameters. Abrupt events during the second half of the deglaciation are caused by a long-term shift in the sign of the Arctic freshwater budget, changes in river routing and/or the opening of ocean passages.
Multiparametric Cardiac Magnetic Resonance Imaging to Discriminate Endomyocardial Biopsy-Proven Chronic Myocarditis From Healed Myocarditis
2024-08-07

A multiparametric CMR protocol allows detection of ongoing myocardial inflammation and discrimination of chronic myocarditis from healed myocarditis, with segmental T<sub>2</sub> mapping and biventricular strain analysis showing higher diagnostic accuracy compared with T<sub>1</sub> mapping, extracellular volume fraction, and LGE. The use of biomarkers (troponin or C-reactive protein) may improve specificity.
Impairment of brain function in a mouse model of Alzheimer's disease during the pre-depositing phase: The role of α7 nicotinic acetylcholine receptors
2024-08-07

Alzheimer's disease (AD) is an age-dependent incurable neurodegenerative disorder accompanied by neuroinflammation, amyloid accumulation, and memory impairment. It begins decades before the first clinical symptoms appear, and identifying early biomarkers is key for developing disease-modifying therapies. We show now in a mouse model of AD that before any amyloid deposition the brains of 1.5-month-old mice contain increased levels of pro-inflammatory cytokines IL-1β and IL-6, decreased levels of nicotinic acetylcholine receptors (nAChRs) in the brain and brain mitochondria and increased amounts of α7 nAChR-bound Aβ<sub>1-42</sub>, along with impaired episodic memory and increased risk of apoptosis. Both acute (1-week-long) and chronic (4-month-long) treatments with α7-selective agonist PNU282987, starting at 1.5 months of age, were well tolerated. The acute treatment did not affect the levels of soluble Aβ<sub>1-42</sub> but consistently upregulated the α7 nAChR expression, decreased the level of α7-Aβ<sub>1-42</sub> complexes, and improved episodic memory of 1.5-month-old mice. The chronic treatment, covering the disease development phase, strongly upregulated the expression of all abundant brain nAChRs, reduced both free and α7-coupled Aβ<sub>1-42</sub> within the brain, had anti-inflammatory and antiapoptotic effects, and potently upregulated cognition, thus identifying α7 nAChRs as both early biomarker and potent therapeutic target for fighting this devastating disease.
Five-year follow-up after a single US-guided high intensity focused ultrasound treatment of breast fibroadenoma
2024-08-07

Abstract The aim of this study was to evaluate the long-term efficacy of a single ultrasound-guided high-intensity focused ultrasound (US-HIFU) treatment in patients with breast fibroadenoma (FA) in terms of volume and pain reduction as well as palpation findings. From december 2013 until november 2014 27 women with a symptomatic FA were treated in one HIFU-session. Follow-up visits were performed after 7 days, 6 months and 1, 2, 3 and 5 years with clinical examination and ultrasound. One year after the procedure, a core needle biopsy of the residual lesion was offered. There was a significant volume reduction 6 months after HIFU from 1083.10 to 347.13 mm 3 (p &lt; 0.0001) with a mean volume reduction ratio (VRR) of 61.63%. Thereafter the FAs showed a further, but no longer significant decrease in size. One patient with an initial incomplete ablation and histologically confirmed persistent vital cells after 1 year showed a strong regrowth after 3 years. Excluding this patient from analysis, the mean VRR at months 12, 24, 36, and 60 was 86.44%, 94.44%, 94.90%, and 97.85%, respectively. Before HIFU, 59.26% of the patients had pain (22.33/100 VAS) which decreased to 6.56/100 after 12 months and remained reduced over the 5 year follow up period. A decrease in palpability from 85.19 to 7.69% was observed within 24 months. A single HIFU intervention let to a substantial reduction in size, pain, and palpability with its most potential effect during the first 12 months. Subsequently, the observed effect remained stable over a 5 year follow up period. Incomplete initial treatment was associated with the risk of regrowth.
Isotopic and trace element record of changing metal source contributions to tropical freshwater Lake Naivasha (Kenya)
2024-08-07

Lake Naivasha, Kenya's second-largest freshwater body is a wetland of international ecological importance and currently subjected to unprecedented anthropogenic influence. The study aims to chronologically reconstruct the main human activities and background weathering reactions that govern metal mobilizations into the lake and their potentially adverse effects on its ecological status. We combine extensive geochemical analyses (major, trace elements, Zn-Pb isotope ratios) in a dated lake sediment record and catchment rocks with remote sensing techniques. Downcore geochemical variations reflect natural ecosystem destabilizations occurring as early as the first half of the 20th century. These coincide with changes towards less radiogenic Pb-isotope values which persist towards the top of the core (<sup>206</sup>Pb/<sup>207</sup>Pb = 1.243 at core base ∼1843, to <sup>206</sup>Pb/<sup>207</sup>Pb = 1.225 at ∼1978). We interpret the land-clearance for agricultural purposes on the Aberdare Range and documented early aviation activities as possible vectors of this early Pb-isotope excursion. The overlapping Pb-isotope signatures between sediment sources and anthropogenic contributions challenges a straightforward deconvolution of the two. Our conservative model calculations suggest, nevertheless, that an addition of up to ∼1.8 % Pb-gasoline influx to the total Pb flux, peaking in the 1980s is able to explain the Pb distribution trend. Homogeneous Zn-isotope compositions in sediments deposited until ∼1970s (δ<sup>66/64</sup>Zn = 0.216-0.225 ‰) do not follow major hydro-climatic events or anthropogenic forcing but likely reflect lake-specific natural cycling. Subsequent higher variations to both heavier (up to δ<sup>66/64</sup>Zn = 0.242 ± 0.005 ‰) and lighter (down to δ<sup>66/64</sup>Zn = 0.184 ± 0.003 ‰) Zn-isotope values are contemporaneous with intensification of large-scale horticultural industry in the catchment. Together with supporting indicators, the lighter Zn-isotope compositions in youngest analysed sediments (21st century) are attributable to increased biological productivity (algal blooms) and ongoing lake eutrophication. Our study demonstrates the applicability of the heavy metal isotope tool to reconstruct human influences on lake environments with complex geological settings such as the East African Rift System.
Elevational Variation in and Environmental Determinants of Fungal Diversity in Forest Ecosystems of Korean Peninsula
2024-08-07

Exploring species diversity along elevational gradients is important for understanding the underlying mechanisms. Our study focused on analyzing the species diversity of fungal communities and their subcommunities at different trophic and taxonomic levels across three high mountains of the Korean Peninsula, each situated in a different climatic zone. Using high-throughput sequencing, we aimed to assess fungal diversity patterns and investigate the primary environmental factors influencing fungal diversity. Our results indicate that soil fungal diversity exhibits different elevational distribution patterns on different mountains, highlighting the combined effects of climate, soil properties, and geographic topology. Notably, the total and available phosphorus contents in the soil emerged as key determinants in explaining the differences in diversity attributed to soil properties. Despite the varied responses of fungal diversity to elevational gradients among different trophic guilds and taxonomic levels, their primary environmental determinants remained remarkably consistent. In particular, total and available phosphorus contents showed significant correlations with the diversity of the majority of the trophic guilds and taxonomic levels. Our study reveals the absence of a uniform diversity pattern along elevational gradients, underscoring the general sensitivity of fungi to soil conditions. By enriching our understanding of fungal diversity dynamics, this research enhances our comprehension of the formation and maintenance of elevational fungal diversity and the response of microbial communities in mountain ecosystems to climate change. This study provides valuable insights for future ecological studies of similar biotic communities.