julilili42/papers huggingface dataset:
A Comparison Of Il-1β, E2 (Pge2), Pai-2 and Tnf-Α Levels in Gingival Crevicular Fluid (Gcf) and Peri-Implant Crevicular Fluid (Picf) from Groups of Patients with Healthy Teeth, Healthy Implants, Periodontitis and Peri-Implantitis
2017-01-01

Objectives:The aim of this study was to analyse simultaneously four markers in gingival crevicular fluid (GCF) and peri-implant crevicular fluid (PICF) from 41 patients in four different groups over a period of six months in order to establish profiles indicating quantitative ratio and potential correlations. Material and Methods:We compared levels of interleukin-1 beta (IL-1ß), prostaglandin E2 (PGE2), specific plasminogen activator inhibitor 2 (PAI-2) and tumor necrosis factor-alpha (TNF-α) in GCF and PICF from four groups of patients: healthy teeth (n=10), healthy implants (n=10), periodontitis (n=11) and peri-implantitis (n=10).Clinical parameters including bacterial flora were quantified by PCR-analysis.The concentration in GCF/PICF of IL-1β, PAI-2, E2 (PGE2) was determined by ELISA, TNF-α by Western blot analysis.Results: Healthy teeth showed significantly higher levels of IL-1β than healthy implants.The mean level of E2 (PGE2) for healthy teeth was twice as high as for healthy implants.Periodontitis and peri-implantitis sites showed highly increased secretion of IL-1ß and E2 (PGE2) resulting in significantly higher levels of IL-1ß in periodontits than in peri-implantitis.Highest TNF-α levels showed in the peri-implantitis group, lowest in healthy teeth.Lowest concentrations of PAI-2 appeared in peri-implantitis, highest in healthy implants. Conclusions:Within the limits of the study it could be hypothesized that the significantly higher levels of IL-1ß and E2 (PGE2) for healthy teeth than for healthy implants could in part be due to the lack of periodontal ligament cells around implants.Another possible explanation could be the anti-inflammatory property of titanium surfaces of implants.
Maintaining a Science Gateway - Lessons Learned from MoSGrid
2017-01-01

We here present the experiences collected maintaining and updating the MoSGrid science gateway over the past years. Insights are provided on a technical and organizational level useful for the design and operation of science gateways in general. The specific challenges faced and solved are considered to be valuable for other communities.
Analysis of N-acetylmuramic acid-6-phosphate (MurNAc-6P) Accumulation by HPLC-MS
2017-01-01

We describe here in detail a high-performance liquid chromatography-mass spectrometry (HPLC-MS)-based method to determine <i>N</i>-acetylmuramic acid-6-phosphate (MurNAc-6P) in bacterial cell extracts. The method can be applied to both Gram-negative and Gram-positive bacteria, and as an example we use <i>Escherichia coli</i> cells in this study. Wild type and mutant cells are grown for a defined time in a medium of choice and harvested by centrifugation. Then the cells are disintegrated and soluble cell extracts are generated. After removal of proteins by precipitation with acetone, the extracts are analyzed by HPLC-MS. Base peak chromatograms of wild type and mutant cell extracts are used to determine a differential ion spectrum that reveals differences in the MurNAc-6P content of the two samples. Determination of peak areas of extracted chromatograms of MurNAc-6P ((M-H)- = 372.070 <i>m/z</i> in negative ion mode) allows quantifying MurNAc-6P levels, that are used to calculate recycling rates of the MurNAc-content of peptidoglycan.
Seven Hellenes and One Christian in the Endless Peace Treaty of 532
2017-01-01

The so-called Endless Peace treaty, signed between Rome and Persia in 532, contained several provisions that regulated issues of population transfer. According to the famous evidence of Agathias of Myrina, in the treaty there was also a clause guaranteeing safety from persecution and the tolerance of religious beliefs in the territory of the Roman Empire for the seven Neoplatonic philosophers returning from their Persian emigration. The present article proposes a re-evaluation of the clause mentioned by Agathias by extracting parallel information from an East-Syriac hagiographical source: an anonymous account of martyrdom of the high-profile Persian Christian convert Mar Grigor. The study deconstructs Agathias' evidence regarding the circumstances of the philosophers' emigration and return, and examines the available set of “conventional” sources on how the Endless Peace treaty regulated the status of different categories of displaced people. The investigation proceeds with an analysis of the Martyrdom of Mar Grigor, arguing for the importance of the East-Syriac hagiographical account for a comprehensive reconstruction of the conditions of the Endless Peace agreement. Assessing information provided by the Martyrdom of Mar Grigor and other available data, the author reveals the high relevance of the East-Syriac evidence for the discussion of the so-called clause of protection. The scope of the article is to demonstrate, for the first time in historiography, that the clause, included in the treaty to protect the seven Hellenic philosophers upon their return to the Christian Roman Empire, was not unilateral. It is suggested that the same diplomatic agreement contained a similar promise of safe conduct for the Christian Persian general, Pīrān-Gušnasp / Mar Grigor, coming back from Roman captivity to Zoroastrian Persia.
Better Complexity Bounds for Cost Register Automata
2017-01-01

Cost register automata (CRAs) are one-way finite automata whose transitions have the side effect that a register is set to the result of applying a state-dependent semiring operation to a pair of registers. Here it is shown that CRAs over the tropical semiring (N U {infinity},\\\\min,+) can simulate polynomial time computation, proving along the way that a naturally defined width-k circuit value problem over the tropical semiring is P-complete. &#13;\\nThen the copyless variant of the CRA, requiring that semiring operations be applied to distinct registers, is shown no more powerful than NC^1 when the semiring is (Z,+,x) or (Gamma^*,max,concat). This relates questions left open in recent work on the complexity of CRA-computable functions to long-standing class separation conjectures in complexity theory, such as NC versus P and NC^1 versus GapNC^1.
Exploiting the sequence diversity of TALE-like repeats to vary the strength of dTALE-promoter interactions
2017-01-01

Abstract Designer transcription activator-like effectors (dTALEs) are programmable transcription factors used to regulate user-defined promoters. The TALE DNA-binding domain is a tandem series of amino acid repeats that each bind one DNA base. Each repeat is 33–35 amino acids long. A residue in the center of each repeat is responsible for defining DNA base specificity and is referred to as the base specificying residue (BSR). Other repeat residues are termed non-BSRs and can contribute to TALE DNA affinity in a non-base-specific manner. Previous dTALE engineering efforts have focused on BSRs. Non-BSRs have received less attention, perhaps because there is almost no non-BSR sequence diversity in natural TALEs. However, more sequence diverse, TALE-like proteins are found in diverse bacterial clades. Here, we show that natural non-BSR sequence diversity of TALEs and TALE-likes can be used to modify DNA-binding strength in a new form of dTALE repeat array that we term variable sequence TALEs (VarSeTALEs). We generated VarSeTALE repeat modules through random assembly of repeat sequences from different origins, while holding BSR composition, and thus base preference, constant. We used two different VarSeTALE design approaches combing either whole repeats from different TALE-like sources (inter-repeat VarSeTALEs) or repeat subunits corresponding to secondary structural elements (intra-repeat VarSeTALEs). VarSeTALE proteins were assayed in bacteria, plant protoplasts and leaf tissues. In each case, VarSeTALEs activated or repressed promoters with a range of activities. Our results indicate that natural non-BSR diversity can be used to diversify the binding strengths of dTALE repeat arrays while keeping target sequences constant.
Actuator development at IAAT for the Cherenkov telescope array medium size telescopes
2017-01-01

The Cherenkov Telescope Array (CTA) will be the future observatory for TeV\\ngamma-ray astronomy. In order to increase the sensitivity and to extend the\\nenergy coverage beyond the capabilities of current facilities, its design\\nconcept features telescopes of three different size classes. Based on the\\nexperience from H.E.S.S. phase II, the Institute for Astronomy and Astrophysics\\nT\\\\"ubingen (IAAT) develops actuators for the mirror control system of the CTA\\nMedium Size Telescopes (MSTs). The goals of this effort are durability, high\\nprecision, and mechanical stability under all environmental conditions. Up to\\nnow, several revisions were developed and the corresponding prototypes were\\nextensively tested. In this contribution our latest design revision proposed\\nfor the CTA MSTs are presented.\\n
An efficient test facility for the Cherenkov telescope array FlashCam readout electronics production
2017-01-01

The Cherenkov Telescope Array (CTA) is the planned next-generation instrument for ground-based gamma-ray astronomy, currently under preparation by a world-wide consortium. The FlashCam group is preparing a photomultiplier-based camera for the Medium Size Telescopes of CTA, with a fully digital Readout System (ROS). For the forthcoming mass production of a substantial number of cameras, efficient test routines for all components are currently under development. We report here on a test facility for the ROS components. A test setup and routines have been developed and an early version of that setup has successfully been used to test a significant fraction of the ROS for the FlashCam camera prototype in January 2016. The test setup with its components and interface, as well as first results, are presented here.
German Wartime Anglophobic Propaganda in France, 1914–1945
2017-01-01

This article explores Anglophobia as a topic in German wartime propaganda aimed at military and civilian communities of France. Anti-British topics were at the centre of a large campaign of propaganda designed to undermine French morale during the two world wars. This study will investigate the goals, the content, and the effects of Anglophobia in France to determine the relation between these two campaigns of psychological warfare. It will be argued that the Nazis and the Vichy regime almost entirely replicated the original production of Anglophobic propaganda in the occupied territories of France during the First World War. This article will also show that Anglophobia almost invariably failed to convince the French population.
Effects of Antimalarial Tafenoquine on Blood Platelet Activity and Survival
2017-01-01

Background/Aims: The 8-aminoquinoline tafenoquine has been shown to be effective against Plasmodia, Leishmania and Trypanosoma. The substance is at least in part effective by triggering apoptosis of the parasites. Moreover, tafenoquine has been shown to trigger eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. The effect of tafenoquine on eryptosis is in part due to stimulation of Ca2+ entry and oxidative stress. Ca2+ entry is a critical event in the activation of blood platelets by thrombin and collagen related peptide (CRP). The present study explored, whether tafenoquine influences Ca2+ entry, activation and apoptosis of blood platelets. Methods: Platelets isolated from wild-type mice were exposed for 30 minutes to tafenoquine (2.5 μg/ml) without or with an additional treatment with thrombin (0.01 U/ml) or CRP (2 μg/ml or 5 μg/ml). Flow cytometry was employed to estimate cytosolic Ca2+-activity ([Ca2+]&lt;Sub&gt;i&lt;/Sub&gt;) from Fluo-3 fluorescence, platelet degranulation from P-selectin abundance, integrin activation from α&lt;Sub&gt;IIb&lt;/Sub&gt;β&lt;Sub&gt;3&lt;/Sub&gt; integrin abundance, phosphatidylserine abundance from annexin-V-binding, relative platelet volume from forward scatter, reactive oxygen species (ROS) from DCF fluorescence, caspase 3 activity with an active caspase-3 Staining kit, and aggregation utilizing staining with CD9-APC and CD9-PE. Results: Both, thrombin (0.01 U/ml) and CRP (2 μg/ml or 5 μg/ml), significantly increased [Ca2+]&lt;Sub&gt;i&lt;/Sub&gt;, P-selectin abundance, active α&lt;Sub&gt;IIb&lt;/Sub&gt;β&lt;Sub&gt;3&lt;/Sub&gt; integrin, and annexin-V-binding, and both significantly decreased platelet volume, activated caspase 3 and stimulated aggregation. Administration of tafenoquine (2.5 μg/ml, 30 min) significantly decreased [Ca2+]&lt;Sub&gt;i&lt;/Sub&gt; both, in the absence and presence of thrombin and CRP. Tafenoquine significantly blunted the effect of thrombin and CRP on [Ca2+]&lt;Sub&gt;i&lt;/Sub&gt;, P-selectin abundance, and active α&lt;Sub&gt;IIb&lt;/Sub&gt;β&lt;Sub&gt;3&lt;/Sub&gt; integrin, but significantly increased ROS and annexin-V-binding, significantly augmented the effect of thrombin on caspase 3 activity and platelet volume and significantly enhanced platelet aggregation. Conclusions: Tafenoquine counteracts thrombin and CRP induced increase of cytosolic Ca2+ activity and platelet activation, but enhances platelet apoptosis and platelet aggregation.
Protein Expression Protocol for an Adenylate Cyclase Anchored by a Vibrio Quorum Sensing Receptor
2017-01-01

The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (<i>Vibrio harveyi</i>) has recently been reported ( Beltz <i>et al.</i>, 2016 ). This protocol describes the expression and membrane preparation for these chimeric proteins.
Resting energy expenditure in girls with Turner syndrome
2017-01-01

Abstract Background: Knowledge concerning energy metabolism in Turner syndrome (TS) is lacking. We compared the resting energy expenditure per fat-free mass (REE/FFM) in TS with other girls with short stature treated with growth hormone (GH) and age-related controls. Methods: We measured prospectively REE by spirometry under fasting conditions in the morning in 85 short prepubertal girls at the start of GH treatment. Diagnoses were TS (n=20), GH deficiency (GHD) (n=38) and small for gestational age (SGA) short stature (n=27). Additionally, 20 age-related controls were studied. Mean ages were 8.3 (TS), 7.1 (GHD), 6.9 (SGA) and 8.5 years (controls). Mean heights were −2.90 (TS), −3.32 (GHD), −3.69 (SGA) and −0.03 standard deviation scores (SDS) (controls). FFM was measured by bioelectrical impedance analysis (BIA). Results: At the start of GH girls with TS showed insignificantly higher REE per FFM (REE/FFM) (mean±SD; 65±9 kcal/kg×day) than did the other female patients (62±9 kcal/kg×day) (p&gt;0.23). The healthy controls had significantly lower REE/FFM (35±4 kcal/kg×day) (p&lt;0.001). Follow-up examination of the patients after 6 or 12 months revealed decreasing REE/FFM in TS (62±9 kcal/kg×day) resulting in comparable REE/FFM in all three patient groups. Conclusions: At baseline short girls with TS had insignificantly higher REE/FFM than short children with SGA or GHD, but in follow-up this difference was not detectable any more. Future studies are necessary to understand this observation.
Photon andπ0pairs production in proton-nucleus and deuteron-nucleus interactions. Results of experiments on internal beams of the Nuclotron
2017-01-01

The results of experiments on photon and π0 pairs production in interactions of 5.5 GeV/c protons, 2.75 and 3.83 GeV/c per nucleon deuterons with carbon and copper nuclei are presented. As previously reported (see. [1] and references therein), along with π0 and η mesons, a resonance structure in the invariant mass spectrum of two photons at Mγγ = 360 ± 7 ± 9 MeV is observed in the reaction dC → γ + γ + X at momentum 2.75 GeV/c per nucleon. Here are some results of testing the signal and results of statistical analysis of the experimental data which allow to explain the lack of appearance of the signal in other experiments [2, 3] and make predictions for possible appearances of the signal in upcoming experiments.
Electroproduction of lightest nucleon resonances up to Q*2=12 GeV*2 in quark models at light front
2017-01-01

\\nThe lightest nucleon resonances are described at light front as mixed states of the 3q cluster (“quark core”) possessing a definite value of the inner orbital momentum L = 0,1 and a hadron molecular state, N+σ or Λ+K. Helicity amplitudes of the resonance electroproduction off the proton are calculated at large Q2 up to 12 GeV2 and compared to the last CLAS data. At this basis we have estimated the probability of quark core in lightest nucleon resonances and predicted the high Q2 behaviour of the resonance electrocoupling.\\n
Design, Implementation and Operation of a Reading Center Platform for Clinical Studies
2017-01-01

Clinical reading centers provide expertise for consistent, centralized analysis of medical data gathered in a distributed context. Accordingly, appropriate software solutions are required for the involved communication and data management processes. In this work, an analysis of general requirements and essential architectural and software design considerations for reading center information systems is provided. The identified patterns have been applied to the implementation of the reading center platform which is currently operated at the Center of Ophthalmology of the University Hospital of T&amp;uuml;bingen.
The stringy Euler number of Calabi–Yau hypersurfaces in toric varieties and the Mavlyutov duality
2017-01-01

We show that minimal models of nondegenerated hypersufaces defined by Laurent polynomials with a -dimensional Newton polytope are Calabi-Yau varieties if and only if the Fine interior of consists of a single lattice point. We give a combinatorial formula for computing the stringy Euler number of . This formula allows to test mirror symmetry in cases when is not a reflexive polytope. In particular we apply this formula to pairs of lattice polytopes that appear in the Mavlyutov's generalization of the polar duality for reflexive polytopes. Some examples of Mavlyutov's dual pairs show that the stringy Euler numbers of the corresponding Calabi-Yau varieties and may not satisfy the expected topological mirror symmetry test: . This shows the necessity of an additional condition on Mavlyutov's pairs .
Algorithm-based arterial blood sampling recognition increasing safety in point-of-care diagnostics
2017-01-01

To detect blood withdrawal for patients with arterial blood pressure monitoring to increase patient safety and provide better sample dating.Blood pressure information obtained from a patient monitor was fed as a real-time data stream to an experimental medical framework. This framework was connected to an analytical application which observes changes in systolic, diastolic and mean pressure to determine anomalies in the continuous data stream. Detection was based on an increased mean blood pressure caused by the closing of the withdrawal three-way tap and an absence of systolic and diastolic measurements during this manipulation. For evaluation of the proposed algorithm, measured data from animal studies in healthy pigs were used.Using this novel approach for processing real-time measurement data of arterial pressure monitoring, the exact time of blood withdrawal could be successfully detected retrospectively and in real-time. The algorithm was able to detect 422 of 434 (97%) blood withdrawals for blood gas analysis in the retrospective analysis of 7 study trials. Additionally, 64 sampling events for other procedures like laboratory and activated clotting time analyses were detected. The proposed algorithm achieved a sensitivity of 0.97, a precision of 0.96 and an F1 score of 0.97.Arterial blood pressure monitoring data can be used to perform an accurate identification of individual blood samplings in order to reduce sample mix-ups and thereby increase patient safety.
A Unified Method for Placing Problems in Polylogarithmic Depth.
2017-01-01

In this work we consider the term evaluation problem which is, given a term over some algebra and a valid input to the term, computing the value of the term on that input. In contrast to previous methods we allow the algebra to be completely general and consider the problem of obtaining an efficient upper bound for this problem. Many variants of the problems where the algebra is well behaved have been studied. For example, the problem over the Boolean semiring or over the semiring (N,+,*). We extend this line of work. Our efficient term evaluation algorithm then serves as a tool for obtaining polylogarithmic depth upper bounds for various well-studied problems. To demonstrate the utility of our result we show new bounds and reprove known results for a large spectrum of problems. In particular, the applications of the algorithm we consider include (but are not restricted to) arithmetic formula evaluation, word problems for tree and visibly pushdown automata, and various problems related to bounded tree-width and clique-width graphs.
Freiräume
2017-01-01

The essays collected in this book are written from a phenomenological and hermeneutical perspective. As philosophical explorations they refer to subject matters given to perception and intuition, and their concepts are reflected within the interpretation of texts.
Sensor Fusion for Semantic Place Labeling
2017-01-01

In order to share knowledge about road situations vehicle-to-vehicle (V2V) communication is used. Autonomous driving vehicles are able to drive and park themself without driver interactions or presence, but are still inefficient about the drivers needs as they don't anticipate the users' behaviour. For instance, if a user wants to stop for quick grocery shopping, there is no need looking for long term parking in far distance, a short-term parking zone near the grocery shop would be adequate. To enable autonomous cars to make such decisions, they could benefit from awareness of their drivers' context. Knowledge about a users' activities and position may help to retrieve context information. To be able to describe the meaning of a visited place for user, we introduce a variant of semantic place labeling based on various sensor data. Data sourced by, e.g. smart-phones or vehicles, is taken into account for gathering personalized context information, including Bluetooth, motion activity, status data and WLAN, and also to compensate for potential inaccuracies. For the classification of place types, over 80 features are generated for each stop. Thereby, geographic data is enriched with point of interest (POI)-information from different location-based context providers. In our experiments, we classify semantic categories of locations using parameter optimized multi-class and smart binary classifiers. An overall accuracy of 88.55% correctly classified stops is achieved using END classifier. A classification without GPS data yields an accuracy of 85.37%, demonstrating that alternative smartphone data can largely compensate for inaccurate localizations based on the fact of 88.55% accuracy, where GPS data was used. Knowing the semantics of a location, the provided context can be used to further personalize autonomous vehicles.