julilili42/papers huggingface dataset:
Disk fragmentation around a massive protostar: Comparison of two 3D codes
2023-02-17

Context . Most massive stars are located in multiple stellar systems. The modeling of disk fragmentation, a mechanism that may plausibly lead to stellar multiplicity, relies on parallel 3D simulation codes whose agreement remains to be evaluated. Aims . Cartesian adaptive-mesh refinement (AMR) and spherical codes have frequently been used in the past decade to study massive star formation. We aim to study how the details of collapse and disk fragmentation depend on these codes. Methods . Using the Cartesian AMR code RAMSES within its self-gravity radiation-hydrodynamical framework, we compared disk fragmentation in a centrally condensed protostellar system to the findings of earlier studies performed on a grid in spherical coordinates using PLUTO . Results . To perform the code comparison, two RAMSES runs were considered, effectively giving qualitatively distinct pictures. On the one hand, when allowing for unlimited sink particle creation with no initial sink, Toomre instability and subsequent gas fragmentation leads to a multiple stellar system whose multiplicity is affected by the grid when triggering fragmentation and via numerically assisted mergers. On the other hand, using a unique, central, fixed-sink particle, a centrally-condensed system forms that is similar to that reported by PLUTO . Hence, the RAMSES-PLUTO comparison was performed with the latter and an agreement between the two codes is found as to the first rotationally supported disk formation, the presence of an accretion shock onto it, and the first fragmentation phase. Gaseous fragments form. The properties of the fragments (i.e., number, mass, and temperature) are dictated by local thermodynamics and are in agreement between the two codes given that the system has entered a highly nonlinear phase. Over the simulations, the stellar accretion rate is made of accretion bursts and continuous accretion on the same order of magnitude. As a minor difference between both codes, the dynamics of the fragments causes the disk structure to be sub-Keplerian in RAMSES , whereas it is found to be Keplerian, thus reaching quiescence, in PLUTO . We attribute this discrepancy to the central star being twice less massive in RAMSES because of the different stellar accretion subgrid models in use - rather than the potential grid effects. Conclusions . In a centrally condensed system, the agreement between RAMSES and PLUTO regarding many of the collapse properties and fragmentation process is good. In contrast, fragmentation occurring in the innermost region and given specific numerical choices (use of sink particles, grid, etc.) have a crucial impact when similar but smooth initial conditions are employed. These aspects prove more crucial than the choice of code, with regard to the system being multiple or centrally condensed.
Pharmacological Inhibition of Glycoprotein VI- and Integrin α2β1-Induced Thrombus Formation Modulated by the Collagen Type
2023-02-17

Background In secondary cardiovascular disease prevention, treatments blocking platelet-derived secondary mediators pose a risk of bleeding. Pharmacological interference of the interaction of platelets with exposed vascular collagens is an attractive alternative, with clinical trials ongoing. Antagonists of the collagen receptors, glycoprotein VI (GPVI), and integrin α2β1, include recombinant GPVI-Fc dimer construct Revacept, 9O12 mAb based on the GPVI-blocking reagent Glenzocimab, Syk tyrosine-kinase inhibitor PRT-060318, and anti-α2β1 mAb 6F1. No direct comparison has been made of the antithrombic potential of these drugs. Methods Using a multiparameter whole-blood microfluidic assay, we compared the effects of Revacept, 9O12-Fab, PRT-060318, or 6F1 mAb intervention with vascular collagens and collagen-related substrates with varying dependencies on GPVI and α2β1. To inform on Revacept binding to collagen, we used fluorescent-labelled anti-GPVI nanobody-28. Results and Conclusion In this first comparison of four inhibitors of platelet–collagen interactions with antithrombotic potential, we find that at arterial shear rate: (1) the thrombus-inhibiting effect of Revacept was restricted to highly GPVI-activating surfaces; (2) 9O12-Fab consistently but partly inhibited thrombus size on all surfaces; (3) effects of GPVI-directed interventions were surpassed by Syk inhibition; and (4) α2β1-directed intervention with 6F1 mAb was strongest for collagens where Revacept and 9O12-Fab were limitedly effective. Our data hence reveal a distinct pharmacological profile for GPVI-binding competition (Revacept), GPVI receptor blockage (9O12-Fab), GPVI signaling (PRT-060318), and α2β1 blockage (6F1 mAb) in flow-dependent thrombus formation, depending on the platelet-activating potential of the collagen substrate. This work thus points to additive antithrombotic action mechanisms of the investigated drugs.
A standardised protocol for blood and cerebrospinal fluid collection and processing for biomarker research in ataxia
2023-02-17

Abstract The European Spinocerebellar Ataxia Type 3/Machado‐Joseph Disease Initiative (ESMI) is a consortium established with the ambition to set up the largest European longitudinal trial‐ready cohort of Spinocerebellar Ataxia Type 3/Machado‐Joseph Disease (SCA3/MJD), the most common autosomal dominantly inherited ataxia worldwide. A major focus of ESMI has been the identification of SCA3/MJD biomarkers to enable future interventional studies. As biosample collection and processing variables significantly impact the outcomes of biomarkers studies, biosampling procedures standardisation was done previously to study visit initiation. Here, we describe the ESMI consensus biosampling protocol, developed within the scope of ESMI, that ultimately might be translated to other neurodegenerative disorders, particularly ataxias, being the first step to protocol harmonisation in the field.
The massless single off-shell scalar box integral — branch cut structure and all-order epsilon expansion
2023-02-17

A bstract We investigate the single off-shell scalar box integral with massless internal lines in dimensional regularization. A special emphasis is given to higher orders in the dimensional regularization parameter epsilon, its branch cut structure, and kinematic limits. Common representations of the box integral introduce superficial branch cuts, which we eliminate to all orders in the epsilon expansion. In the literature so far a satisfactory solution for this issue only exists up to finite order in the epsilon expansion. However, for certain calculations at NNLO in perturbation theory, higher orders in epsilon of this integral are required. In this paper, we present results to all orders in epsilon in terms of single-valued polylogarithms and explicitly determine the real and imaginary part of the box integral in all kinematic regions.
Shared pattern of impaired social communication and cognitive ability in the youth brain across diagnostic boundaries
2023-02-17

These results reveal dimensions of brain-behaviour associations across diagnostic boundaries, highlighting potent disorder-general patterns as the most prominent. In addition to providing biologically informed patterns of relevant behavioural factors for mental illness, this contributes to a growing body of evidence in favour of transdiagnostic approaches to prevention and intervention.
Ubuntu and the Problem of Belonging
2023-02-17

ABSTRACTThis paper proposes an original ubuntu-inspired account of human-animal moral status for engaging the problem of belongingness—the ethico-ecological community view. This account embodies two integrated features: locatedness and relationality. While locatedness prompts us to attend to the embeddedness of beings in the built and natural environment, relationality allows the discussion to focus on human-nonhuman interdependencies. I argue that a deep sense of both features prompts us to move the moral status conversation away from capacities to a non-capacity-based approach, thereby helping to disrupt unjustified human moral superiority over animals in a way that commits us to environmental flourishing.KEYWORDS: Belongingenvironmentmoral statusUbuntu AcknowledgmentsI wish to thank the Eberhard Karls University of Tubingen (UT) for supporting my research with a fellowship. I also wish to appreciate Dr. Niels Weidtmann and other colleagues at the College of Fellows, UT for their feedback on an earlier draft of this article.Disclosure StatementNo potential conflict of interest was reported by the author.Notes1. The notion of belonging here points us to the individuals and groups that must be treated as deserving moral consideration—all things being equal.2. I use 'location' in a purely geographical site, independent of what we feel about it. I should emphasize here that I am neither proposing nor defending Aristotle's conflation of both concepts. I only draw on his view to point out that a being is always situated in a site. This is what I mean by the term 'locatedness'.
Acute deletion of the central MR/GR steroid receptor correlates with changes in LTP, auditory neural gain, and GC-A cGMP signaling
2023-02-17

The complex mechanism by which stress can affect sensory processes such as hearing is still poorly understood. In a previous study, the mineralocorticoid (MR) and/or glucocorticoid receptor (GR) were deleted in frontal brain regions but not cochlear regions using a CaMKIIα-based tamoxifen-inducible Cre ERT2 /loxP approach. These mice exhibit either a diminished (MR TMX cKO) or disinhibited (GR TMX cKO) auditory nerve activity. In the present study, we observed that mice differentially were (MR TMX cKO) or were not (GR TMX cKO) able to compensate for altered auditory nerve activity in the central auditory pathway. As previous findings demonstrated a link between central auditory compensation and memory-dependent adaptation processes, we analyzed hippocampal paired-pulse facilitation (PPF) and long-term potentiation (LTP). To determine which molecular mechanisms may impact differences in synaptic plasticity, we analyzed Arc/Arg3.1, known to control AMPA receptor trafficking, as well as regulators of tissue perfusion and energy consumption (NO-GC and GC-A). We observed that the changes in PPF of MR TMX cKOs mirrored the changes in their auditory nerve activity, whereas changes in the LTP of MR TMX cKOs and GR TMX cKOs mirrored instead the changes in their central compensation capacity. Enhanced GR expression levels in MR TMX cKOs suggest that MRs typically suppress GR expression. We observed that hippocampal LTP, GC-A mRNA expression levels, and ABR wave IV/I ratio were all enhanced in animals with elevated GR (MR TMX cKOs) but were all lower or not mobilized in animals with impaired GR expression levels (GR TMX cKOs and MRGR TMX cKOs). This suggests that GC-A may link LTP and auditory neural gain through GR-dependent processes. In addition, enhanced NO-GC expression levels in MR, GR, and MRGR TMX cKOs suggest that both receptors suppress NO-GC; on the other hand, elevated Arc/Arg3.1 levels in MR TMX cKOs and MRGR TMX cKOs but not GR TMX cKOs suggest that MR suppresses Arc/Arg3.1 expression levels. Conclusively, MR through GR inhibition may define the threshold for hemodynamic responses for LTP and auditory neural gain associated with GC-A.
The Female Pelvis Is Associated with a Lateralized Ischium and a Reduced Ischiofemoral Space
2023-02-17

Background: Pelvi-femoral conflicts are increasingly recognized for their explanatory role in the pathology of extra-articular hip impingement. Ischiofemoral impingement (IFI) is a type of impingement between the femur and the ischium that causes high femoral antetorsion and valgus femoral neck orientation. It is unknown whether obstetric adaptation of the female pelvis renders the female hip at a higher risk of sustaining IFI. The aim of this study was to determine the influence of the pelvic morphology on the ischiofemoral space (IFS). Methods: Plain radiographs of healthy individuals with no symptomatic hip disease were obtained in a functional standing position in a standardized manner and utilized for measurement of the interischial and ischiofemoral widths, subpubic angle, and centrum collum diaphyseal (CCD) angle. Linear regression was performed to determine the influence of morphometric measures on the ischiofemoral space. Results: Sixty-five radiographs (34 females and 31 males) were included. The cohort was stratified according to gender. Significant gender-related differences were noted regarding the ischiofemoral distance (31% increase in males, p &lt; 0.001), pubic-arc angle (30% increased in females, p &lt; 0.001), and the interischial space (7% increase in females, p &lt; 0.001). CCD did not significantly differ between genders (p = 0.2). Factors influencing the IFS include the pubic-arc angle (β = −0.01 (CI −0.02–−0.00), p = 0.003), interischial distance (β = −0.11 (CI −0.23–−0.00), p = 0.049) and CCD (β = −0.06 (CI −0.09–−0.04), p &lt; 0.001). Conclusions: Obstetric adaptation is associated with an increased subpubic angle that shifts the ischia laterally and away from the symphysis. The resultant reduction in the ischiofemoral space renders the female pelvis at a higher risk for a pelvi-femoral conflict, or more precisely, an ischiofemoral conflict, due to the reduced ischiofemoral space of the hip. The CCD angle of the femur was shown not to be gender specific. However, the CCD angle demonstrates an influence on the ischiofemoral space, rendering the proximal femur a target for corresponding osteotomies.
How to do a 3D uterus ultrasound?
2023-02-17

Abstract Three-dimensional (3D) ultrasound is an invaluable tool in the detection and evaluation of many uterine anomalies and improves upon the traditional approach of two-dimensional (2D) ultrasonography. We aim to describe an easy way of assessing the uterine coronal plane using the basic three-dimensional ultrasound in everyday gynecological practice.
K2S Challenge: From Undersampled K-Space to Automatic Segmentation
2023-02-18

Magnetic Resonance Imaging (MRI) offers strong soft tissue contrast but suffers from long acquisition times and requires tedious annotation from radiologists. Traditionally, these challenges have been addressed separately with reconstruction and image analysis algorithms. To see if performance could be improved by treating both as end-to-end, we hosted the K2S challenge, in which challenge participants segmented knee bones and cartilage from 8× undersampled k-space. We curated the 300-patient K2S dataset of multicoil raw k-space and radiologist quality-checked segmentations. 87 teams registered for the challenge and there were 12 submissions, varying in methodologies from serial reconstruction and segmentation to end-to-end networks to another that eschewed a reconstruction algorithm altogether. Four teams produced strong submissions, with the winner having a weighted Dice Similarity Coefficient of 0.910 ± 0.021 across knee bones and cartilage. Interestingly, there was no correlation between reconstruction and segmentation metrics. Further analysis showed the top four submissions were suitable for downstream biomarker analysis, largely preserving cartilage thicknesses and key bone shape features with respect to ground truth. K2S thus showed the value in considering reconstruction and image analysis as end-to-end tasks, as this leaves room for optimization while more realistically reflecting the long-term use case of tools being developed by the MR community.
Inhaled prostacyclin therapy in the acute respiratory distress syndrome: a randomized controlled multicenter trial
2023-02-18

Abstract Background Acute respiratory distress syndrome (ARDS) results in significant hypoxia, and ARDS is the central pathology of COVID-19. Inhaled prostacyclin has been proposed as a therapy for ARDS, but data regarding its role in this syndrome are unavailable. Therefore, we investigated whether inhaled prostacyclin would affect the oxygenation and survival of patients suffering from ARDS. Methods We performed a prospective randomized controlled single-blind multicenter trial across Germany. The trial was conducted from March 2019 with final follow-up on 12th of August 2021. Patients with moderate to severe ARDS were included and randomized to receive either inhaled prostacyclin (3 times/day for 5 days) or sodium chloride (Placebo). The primary outcome was the oxygenation index in the intervention and control groups on Day 5 of therapy. Secondary outcomes were mortality, secondary organ failure, disease severity and adverse events. Results Of 707 patients approached 150 patients were randomized to receive inhaled prostacyclin (n = 73) or sodium chloride (n = 77). Data from 144 patients were analyzed. The baseline PaO 2 /FiO 2 ratio did not differ between groups. The primary analysis of the study was negative, and prostacyclin improved oxygenation by 20 mmHg more than Placebo (p = 0.17). Secondary analysis showed that the oxygenation was significantly improved in patients with ARDS who were COVID-19-positive (34 mmHg, p = 0.04). Mortality did not differ between groups. Secondary organ failure and adverse events were similar in the intervention and control groups. Conclusions The primary result of our study was negative. Our data suggest that inhaled prostacyclin might be beneficial treatment in patients with COVID-19 induced ARDS. Trial registration: The study was approved by the Institutional Review Board of the Research Ethics Committee of the University of Tübingen (899/2018AMG1) and the corresponding ethical review boards of all participating centers. The trial was also approved by the Federal Institute for Drugs and Medical Devices (BfArM, EudraCT No. 2016003168-37) and registered at clinicaltrials.gov (NCT03111212) on April 6th 2017.
Interplay between autophagy and proteasome during protein turnover
2023-02-19

Protein homeostasis is epitomized by an equilibrium between protein biosynthesis and degradation: the 'life and death' of proteins. Approximately one-third of newly synthesized proteins are degraded. As such, protein turnover is required to maintain cellular integrity and survival. Autophagy and the ubiquitin-proteasome system (UPS) are the two principal degradation pathways in eukaryotes. Both pathways orchestrate many cellular processes during development and upon environmental stimuli. Ubiquitination of degradation targets is used as a 'death' signal by both processes. Recent findings revealed a direct functional link between both pathways. Here, we summarize key findings in the field of protein homeostasis, with an emphasis on the newly revealed crosstalk between both degradation machineries and how it is decided which pathway facilitates target degradation.
Bright and Photostable TADF‐Emitting Zirconium(IV) Pyridinedipyrrolide Complexes: Efficient Dyes for Decay Time‐Based Temperature Sensing and Imaging
2023-02-19

Abstract Luminescence thermometry represents a technique of choice for measurements in small objects and imaging of temperature distribution. However, most state‐of‐the‐art luminescent probes are limited in spectral characteristics, brightness, photostability, and sensitivity. Molecular thermometers of the new generation utilizing air and moisture‐stable zirconium(IV) pyridinedipyrrolide complexes can address all these limitations. The dyes emit pure thermally activated delayed fluorescence without any prompt fluorescence and show a unique combination of attractive features: a) visible light excitation and emission in the orange/red region, b) high luminescence brightness (quantum yields ≈0.5 in toluene and 0.8–1.0 in polystyrene matrix), c) excellent photostability, d) suitability for two‐photon excitation and e) mono‐exponential decay on the order of tens to hundreds of microseconds with strongly temperature‐dependent lifetimes (between −2.5 and −2.9% K −1 in polystyrene at 25 °C). Immobilization in gas‐blocking polymers yields sensing materials for self‐referenced decay time read‐out that are manufactured in two common formats: planar optodes and water‐dispersible nanoparticles. Positively charged nanoparticles are demonstrated to be suitable for nanothermometry in live cells and multicellular spheroids. Negatively charged nanoparticles represent advanced analytical tools for imaging temperature gradients in samples of small volumes such as microfluidic devices.
Ticks and Rickettsiae Associated with Wild Animals Sold in Bush Meat Markets in Cameroon
2023-02-19

Ticks are obligate blood-sucking parasites of wild animals and transmit many zoonotic microorganisms that can spread to domesticated animals and then to humans. In Cameroon, little is known about tick diversity among wildlife, especially for animals which are hunted for human consumption. Therefore, this survey was undertaken to investigate tick and Rickettsia species diversity parasitizing the wild animals sold in bush meat markets in Cameroon. In total, 686 ticks were collected and identified to the species level based on morphology, and some were genetically analyzed using the 16S rRNA gene. Eighteen tick species belonging to five genera were identified: Amblyomma spp. (Amblyomma compressum, Amblyomma flavomaculatum, and Amblyomma variegatum), Haemaphysalis spp. (Haemaphysalis camicasi, Haemaphysalis houyi, Haemaphysalis leachi, and Haemaphysalis parmata), Hyalomma spp. (Hyalomma nitidum, Hyalomma rufipes, and Hyalomma truncatum), Ixodes spp. (Ixodes rasus and Ixodes moreli), and Rhipicephalus spp. (Rhipicephalus guilhoni, Rhipicephalus moucheti, Rhipicephalus muhsamae, Rhipicephalus microplus, Rhipicephalus camicasi, and Rhipicephalus linnaei). In terms of Rickettsia important for public health, two Rickettsia spp., namely Rickettsia aeschlimannii and Rickettsia africae, were detected in Hyalomma spp. and Amblyomma spp., respectively. Distinct tick–pathogen patterns were present for divergent sequences of R. africae associated with exclusively A. variegatum vectors (type strain) versus vectors comprising A. compressum, A. flavomaculatum, and A. variegatum. This suggests possible effects of vector species population dynamics on pathogen population circulation dynamics. Furthermore, Candidatus Rickettsia africaustralis was detected for the first time in Cameroon in I. rasus. This study highlights the high diversity of ticks among wildlife sold in bush meat markets in Cameroon.
Feasibility, efficacy, and functional relevance of automated auditory closed‐loop suppression of slow‐wave sleep in humans
2023-02-19

Summary Slow‐wave sleep (SWS) is a fundamental physiological process, and its modulation is of interest for basic science and clinical applications. However, automatised protocols for the suppression of SWS are lacking. We describe the development of a novel protocol for the automated detection (based on the whole head topography of frontal slow waves) and suppression of SWS (through closed‐loop modulated randomised pulsed noise), and assessed the feasibility, efficacy and functional relevance compared to sham stimulation in 15 healthy young adults in a repeated‐measure sleep laboratory study. Auditory compared to sham stimulation resulted in a highly significant reduction of SWS by 30% without affecting total sleep time. The reduction of SWS was associated with an increase in lighter non‐rapid eye movement sleep and a shift of slow‐wave activity towards the end of the night, indicative of a homeostatic response and functional relevance. Still, cumulative slow‐wave activity across the night was significantly reduced by 23%. Undisturbed sleep led to an evening to morning reduction of wake electroencephalographic theta activity, thought to reflect synaptic downscaling during SWS, while suppression of SWS inhibited this dissipation. We provide evidence for the feasibility, efficacy, and functional relevance of a novel fully automated protocol for SWS suppression based on auditory closed‐loop stimulation. Future work is needed to further test for functional relevance and potential clinical applications.