julilili42/papers huggingface dataset:
Viral Recognition and Evasion in Plants
2024-07-22

Viruses, causal agents of devastating diseases in plants, are obligate intracellular pathogens composed of a nucleic acid genome and a limited number of viral proteins. The diversity of plant viruses, their diminutive molecular nature, and their symplastic localization pose challenges to understanding the interplay between these pathogens and their hosts in the currently accepted framework of plant innate immunity. It is clear, nevertheless, that plants can recognize the presence of a virus and activate antiviral immune responses, although our knowledge of the breadth of invasion signals and the underpinning sensing events is far from complete. Below, I discuss some of the demonstrated or hypothesized mechanisms enabling viral recognition in plants, the step preceding the onset of antiviral immunity, as well as the strategies viruses have evolved to evade or suppress their detection.
Contribution of El Niño Southern Oscillation (ENSO) Diversity to Low‐Frequency Changes in ENSO Variance
2024-07-22

Abstract El Niño Southern Oscillation (ENSO) diversity is characterized based on the longitudinal location of maximum sea surface temperature anomalies (SSTA) and amplitude in the tropical Pacific, as Central Pacific events are typically weaker than Eastern Pacific events. SSTA pattern and intensity undergo low‐frequency modulations, affecting ENSO prediction skill and remote impacts, and resulting in low‐frequency changes in ENSO variance. Yet, how different ENSO types contribute to these decadal variance changes remains unclear. Here, we decompose the low‐frequency changes of ENSO variance into contributions from ENSO diversity categories. We propose a fuzzy clustering of monthly SSTA to allow for non‐binary event category memberships, where each event can belong to different clusters. Our approach identifies two La Niña and three El Niño categories and shows that the major shift of ENSO variance in the mid‐1970s was associated with an increasing likelihood of strong La Niña and extreme El Niño events.
The Spectrum of CAR Cellular Effectors: Modes of Action in Anti-Tumor Immunity
2024-07-22

Chimeric antigen receptor-T cells have spearheaded the field of adoptive cell therapy and have shown remarkable results in treating hematological neoplasia. Because of the different biology of solid tumors compared to hematological tumors, response rates of CAR-T cells could not be transferred to solid entities yet. CAR engineering has added co-stimulatory domains, transgenic cytokines and switch receptors to improve performance and persistence in a hostile tumor microenvironment, but because of the inherent cell type limitations of CAR-T cells, including HLA incompatibility, toxicities (cytokine release syndrome, neurotoxicity) and high costs due to the logistically challenging preparation process for autologous cells, the use of alternative immune cells is gaining traction. NK cells and γδ T cells that do not need HLA compatibility or macrophages and dendritic cells with additional properties such as phagocytosis or antigen presentation are increasingly seen as cellular vehicles with potential for application. As these cells possess distinct properties, clinicians and researchers need a thorough understanding of their peculiarities and commonalities. This review will compare these different cell types and their specific modes of action seen upon CAR activation.
Believe It or Not? Investigating the Credibility of Voice Assistants in the Context of Social Roles and Relationship Types
2024-07-22

Recent advancements in artificial intelligence have empowered voice assistants to comprehend user input and provide contextually relevant responses more effectively. This paper aims to explore people's relationship with voice assistants, examining factors like the sense of power, control, closeness, and parasocial relationships, and how relationship types relate to the credibility of voice assistants. To investigate this, we built on the foundation of interpersonal relationships and applied parasocial relationship theory as a framework for understanding the human–agent relationship. We conducted an online survey (N = 393). Our results show that people mainly perceive their relationship with voice assistants as either a servant–master relationship, student–teacher relationship, or equal partnership. This study highlights that voice assistants' mimicry of human characteristics can make them more than tools—they can also be perceived as servants, teachers, or equal partners. Notably, a significant association exists between relationship type and credibility judgments towards voice assistants.
The role of FraI in cell–cell communication and differentiation in the hormogonia-forming cyanobacterium Nostoc punctiforme
2024-07-22

ABSTRACT Multicellular cyanobacteria, like Nostoc punctiforme, rely on septal junctions for cell–cell communication, which is crucial for coordinating various physiological processes including differentiation of N 2 -fixing heterocysts, spore-like akinetes, and hormogonia—short, motile filaments important for dispersal. In this study, we functionally characterize a protein, encoded by gene Npun_F4142, which in a random mutagenesis approach, initially showed a motility-related function. The reconstructed Npun_F4142 knockout mutant exhibits further distinct phenotypic traits, including altered hormogonia formation with significant reduced motility, inability to differentiate heterocysts and filament fragmentation. For that reason, we named the protein FraI (fragmentation phenotype). The mutant displays severely impaired cell–cell communication, due to almost complete absence of the nanopore array in the septal cell wall, which is an essential part of the septal junctions. Despite lack of communication, hormogonia in the Δ fraI mutant maintain motility and phototactic behavior, even though less pronounced than the wild type (WT). This suggests an alternative mechanism for coordinated movement beyond septal junctions. Our study underscores the significance of FraI in nanopore formation and cell differentiation, and provides additional evidence for the importance of septal junction formation and communication in various differentiation traits of cyanobacteria. The findings contribute to a deeper understanding of the regulatory networks governing multicellular cyanobacterial behavior, with implications for broader insights into microbial multicellularity. IMPORTANCE The filament-forming cyanobacterium Nostoc punctiforme serves as a valuable model for studying cell differentiation, including the formation of nitrogen-fixing heterocysts and hormogonia. Hormogonia filaments play a crucial role in dispersal and plant colonization, providing a nitrogen source through atmospheric nitrogen fixation, thus holding promise for fertilizer-free agriculture. The coordination among the hormogonia cells enabling uniform movement toward the positive signal remains poorly understood. This study investigates the role of septal junction-mediated communication in hormogonia differentiation and motility, by studying a Δ fraI mutant with significantly impaired communication. Surprisingly, impaired communication does not abolish synchronized filament movement, suggesting an alternative coordination mechanism. These findings deepen our understanding of cyanobacterial biology and have broader implications for multicellular behavior in prokaryotes.
An ambition at a crossroads: Transiting out of the game in amateur and semi-professional football in Nigeria
2024-07-22

Thousands of youths play in amateur and semi-professional clubs in Nigeria with the hope of achieving a professional contract or transnational migration through football. For the majority, however, the social and spatial mobility that is hoped for is never realised. This means that they would have to terminate their pursuit and transition into alternative adulthood pathways. Through semi-structured interviews with former players, this study explored how athletes in amateur and semi-professional football in Nigeria experience and navigate the termination process and transition out of the game. The findings revealed that career termination may develop in a non-linear trajectory as athletes consider their chances of a professional career, age and the obligations of adulthood and intergenerational care. Generally, the athletes navigated the process without significant support from their clubs or other institutions. However, they made use of the social capital and relationships built from their involvement in football (e.g. going into coaching) or leveraged familial ties to secure alternative livelihood opportunities. While participants in this study showed a proactive quest narrative to rebuild their lives away from football, some reported experiences of a chaos narrative among former colleagues, especially manifesting in alcoholism. The findings of this study underscored the need for frameworks for dual career planning for athletes alongside their athletic education and making provisions for psychosocial support for players transitioning out of the game.
A juvenile pleurosaurid (Lepidosauria: Rhynchocephalia) from the Tithonian of the Mörnsheim Formation, Germany
2024-07-22

Abstract Late Jurassic rhynchocephalians from the Solnhofen Archipelago have been known for almost two centuries. The number of specimens and taxa is constantly increasing, but little is known about the ontogeny of these animals. The well‐documented marine taxon Pleurosaurus is one of such cases. With over 15 described (and many more undescribed) specimens, there were no unambiguous juveniles so far. Some authors have argued that Acrosaurus , another common component of the Solnhofen Archipelago herpetofauna, might represent an early ontogenetic stage of Pleurosaurus , but the lack of proper descriptions for this taxon makes this assignment tentative, at best. Here, we describe the first unambiguous post‐hatchling juvenile of Pleurosaurus and tentatively attribute it to Pleurosaurus cf. P. ginsburgi . The new specimen comes from the Lower Tithonian of the Mörnsheim Formation, Germany. This specimen is small, disarticulated, and incomplete, but preserves several of its craniomandibular bones and presacral vertebrae. It shares with Pleurosaurus a set of diagnostic features, such as an elongated and triangular skull, a low anterior flange in its dentition, and an elongated axial skeleton. It can be identified as a juvenile due to the presence of an unworn dentition, well‐spaced posteriormost dentary teeth, a large gap between the last teeth and the coronoid process of the dentary, and poorly ossified vertebrae with unfused neural arches. Acrosaurus shares many anatomical features with both this specimen and Pleurosaurus , which could indicate that the two genera are indeed synonyms. The early ontogenetic stage inferred for the new Pleurosaurus specimen argues for an even earlier ontogenetic placement for specimens referred to Acrosaurus , the latter possibly pertaining to hatchlings.
Cultural Diversity Climate in School: A Meta-Analytic Review of Its Relationships With Intergroup, Academic, and Socioemotional Outcomes
2024-07-22

This first-of-its-kind meta-analysis (N = 79 studies; 56,552 students; k = 640 effects) provides acomprehensive assessment of five cultural diversity climate approaches that capture differentways of addressing cultural diversity in K-12 schools. We examined how intergroup contacttheory’s optimal contact conditions, multiculturalism climate, colorblind climate, criticalconsciousness climate, and polyculturalism climate were associated with children’s andadolescents’ intergroup outcomes (intergroup attitudes, cross-group friendships, experienceddiscrimination), academic outcomes (academic achievement, motivation, engagement), andsocioemotional outcomes (belonging, well-being). Results from meta-analytic random-effectsmodels revealed the largest and most consistent effects for optimal contact conditions, withsmall-to-medium-sized effects and significant relationships with all outcomes. Multiculturalismclimate was significantly and positively related to intergroup attitudes, achievement, motivation,and belonging (mostly, these were small effect sizes). Critical consciousness climate (small effectsizes) and polyculturalism climate (small-to-medium effect sizes) were correlated with bothacademic and socioemotional outcomes. Colorblind climate was not significantly associated withany outcomes. Moderator analyses revealed that contact conditions exhibited larger effects insecondary education compared with primary education and in the US compared with Europe. Thepercentage of majority group members moderated some relationships (e.g., contact conditionshad smaller effects when there were more majority group members in the sample). Significantlylarger effects emerged for student-reported colorblind climate measures than for teacher-reportedmeasures. Overall, this meta-analysis provides a highly nuanced view of the most robust evidencefor the associations between cultural diversity climate and outcomes that are critical for positivechild and youth development to date.
Glyphosate contamination in European rivers not from herbicide application?
2024-07-23

The most widely used herbicide glyphosate contaminates surface waters around the globe. Both agriculture and urban applications are discussed as sources for glyphosate. To better delineate these sources, we investigated long-term time series of concentrations of glyphosate and its main transformation product aminomethylphosphonic acid (AMPA) in a large meta-analysis of about 100 sites in the USA and Europe. The U.S. data reveal pulses of glyphosate and AMPA when the discharge of the river is high, likely indicating mobilization by rain after herbicide application. In contrast, European concentration patterns of glyphosate and AMPA show a typical cyclic-seasonal component in their concentration patterns, correlating with patterns of wastewater markers such as pharmaceuticals, which is consistent with the frequent detection of these compounds in wastewater treatment plants. Our large meta-analysis clearly shows that for more than a decade, municipal wastewater was a very important source of glyphosate. In addition, European river water data show rather high and constant base mass fluxes of glyphosate all over the year, not expected from herbicide application. From our meta-analysis, we define criteria for a source of glyphosate, which was hidden so far. AMPA is known to be a transformation product not only of glyphosate but also of aminopolyphosphonates used as antiscalants in many applications. As they are used in laundry detergents in Europe but not in the USA, we hypothesize that glyphosate may also be a transformation product of aminopolyphosphonates.
The structure and mechanics of the cell cortex depend on the location and adhesion state
2024-07-23

Cells exist in different phenotypes and can transition between them. A phenotype may be characterized by many different aspects. Here, we focus on the example of whether the cell is adhered or suspended and choose particular parameters related to the structure and mechanics of the actin cortex. The cortex is essential to cell mechanics, morphology, and function, such as for adhesion, migration, and division of animal cells. To predict and control cellular functions and prevent malfunctioning, it is necessary to understand the actin cortex. The structure of the cortex governs cell mechanics; however, the relationship between the architecture and mechanics of the cortex is not yet well enough understood to be able to predict one from the other. Therefore, we quantitatively measured structural and mechanical cortex parameters, including cortical thickness, cortex mesh size, actin bundling, and cortex stiffness. These measurements required developing a combination of measurement techniques in scanning electron, expansion, confocal, and atomic force microscopy. We found that the structure and mechanics of the cortex of cells in interphase are different depending on whether the cell is suspended or adhered. We deduced general correlations between structural and mechanical properties and show how these findings can be explained within the framework of semiflexible polymer network theory. We tested the model predictions by perturbing the properties of the actin within the cortex using compounds. Our work provides an important step toward predictions of cell mechanics from cortical structures and suggests how cortex remodeling between different phenotypes impacts the mechanical properties of cells.
Ubiquitin-Proteasome System in the Different Stages of Dominantly Inherited Alzheimer’s Disease
2024-07-23

<title>Abstract</title> This study explored the role of the ubiquitin-proteasome system (UPS) in dominantly inherited Alzheimer’s disease (DIAD) by examining changes in cerebrospinal fluid (CSF) levels of UPS proteins along with disease progression, AD imaging biomarkers (PiB PET, tau PET), neurodegeneration imaging measures (MRI, FDG PET), and Clinical Dementia Rating® (CDR®). Using the SOMAscan assay, we detected subtle increases in specific ubiquitin enzymes associated with proteostasis in mutation carriers (MCs) up to two decades before the estimated symptom onset. This was followed by more pronounced elevations of UPS-activating enzymes, including E2 and E3 proteins, and ubiquitin-related modifiers. Our findings also demonstrated consistent correlations between UPS proteins and CSF biomarkers such as Aβ42/40 ratio, total tau, various phosphorylated tau species to total tau ratios (ptau181/T181, ptauT205/T205, ptauS202/S202, ptauT217/T217), and MTBR-tau243, alongside Neurofilament light chain (NfL) and the CDR®. Notably, a positive association was observed with imaging markers (PiB PET, tau PET) and a negative correlation with markers of neurodegeneration (FDG PET, MRI), highlighting a significant link between UPS dysregulation and neurodegenerative processes. The correlations suggest that the increase in multiple UPS proteins with rising tau levels and tau-tangle associated markers, indicating a potential role for the UPS in relation to misfolded tau/neurofibrillary tangles (NFTs) and symptom onset. These findings indicate that elevated CSF UPS proteins in DIAD MCs could serve as early indicators of disease progression and suggest a link between UPS dysregulation and amyloid plaque, tau tangles formation, implicating the UPS as a potential therapeutic target in AD pathogenesis.
Recurring Transient Tooth Pain as Newly Described Symptom of Migratory Loiasis: A Mixed-Methods Study in Rural Gabon
2024-07-23

Loiasis, a filarial pathogen exclusively endemic in central and western Africa, causes a wide spectrum of symptoms. Understanding the breadth of its clinical manifestations is of importance for adequate patient care and to understand its disease burden. Recurring transient pain in the oral cavity was reported as a self-perceived symptom of loiasis in in-depth interviews of patients in a high transmission region in Gabon. Pain was described as stabbing in character and transient for a few days in its temporal course. A quantitative epidemiological survey indicated that transient tooth pain was experienced by 22% of patients infected with Loa loa . Among those individuals, it was exclusively reported by patients suffering from migratory loiasis (24%). Similar findings have been previously described for other filarial pathogens, indicating that transient swellings of the periodontium and the soft tissue of the oral cavity may explain this symptom reported by patients with migratory loiasis.
Couplings of torsional and shear oscillations in a neutron star crust
2024-07-23

Mature neutron stars are thought to be sufficiently cold that nuclei in the outer layers freeze, solidifying a crust. Crustal elasticity allows the star to support a set of seismic modes, such as torsional oscillations. These axial-parity modes can couple to the polar sector in a number of ways, for example via rotation or a magnetic field. Even in a static, spherically symmetric star, however, these modes can couple at nonlinear order. In this study, such couplings in the crust are examined for the first time: we derive the axisymmetric perturbation equations for second-order axial eigenfunctions, which are sourced by axial-polar couplings at first order, and solve the resulting equations in the time domain. Through our studies, we find that the second-order spectrum contains additional oscillation modes, not predicted by the linear analysis with either axial or polar perturbations, which can be excited to relatively large amplitudes.
Ultrasound-guided localization of the radial nerve along the humerus: providing reference points for safer upper arm surgery
2024-07-23

Abstract Purpose The close proximity of the radial nerve to the humerus poses a risk during upper arm surgery. Although the general course of the radial nerve is well-known, its exact position in relation to anatomical reference points remains poorly investigated. This study aimed to develop a standardized protocol for the sonographic and clinical identification of the radial nerve in the upper arm. The ultimate goal is to assist surgeons in avoiding iatrogenic radial nerve palsy. Methods A total of 76 measurements were performed in 38 volunteers (both sides). Ultrasound measurements were performed using a linear transducer (10 MHz) to identify the radial nerve at two key points: RD (where the radial nerve crosses the dorsal surface of the humerus) and RL (where the radial nerve crosses the lateral aspect of the humerus). Distances from specific reference points (acromion, lateral epicondyle, medial epicondyle, olecranon fossa) to RD and RL were measured, and the angle between the course of the nerve and the humeral axis was recorded. Humeral length was defined as the distance between the posterodorsal corner of the acromion and the lateral epicondyle. Results The distance from the lateral epicondyle to RD was on average 15.5 cm ± 1.3, corresponding to 50% of the humeral length. The distance from the lateral epicondyle to RL was on average 6.7 cm ± 0.8, corresponding to 21% of the humeral length. The course of the nerve between RD and RL showed an average angulation of 37° to the anatomical axis of the humerus. Gender, BMI, dominant hand, and arm thickness did not correlate with the distances to RD or RL. Measurements were consistent between the left and right side. Conclusion The radial nerve can typically be identified by employing a 1/2 and 1/5 ratio on the dorsal and lateral aspects of the humerus. Due to slight variations in individual anatomy, the utilization of ultrasound-assisted visualization presents a valuable and straightforward approach to mitigate the risk of iatrogenic radial nerve palsy during upper arm surgery. This study introduces an easy and fast protocol for this purpose.