24. (1) A person who engages in aquaculture without a licence is exempt from subsection 47 (1) of the Act if the person engages in aquaculture in any of the following circumstances: 1.  The person cultures fish in an aquarium for personal use. 2.  The person cultures fish in an aquarium for the purpose of engaging in the aquarium trade. 3.  Subject to subsection (1.1), the person cultures fish in an aquarium located at an educational institution or facility or at a zoo for one of the following purposes: i.  to exhibit the fish at the educational institution or facility or at the zoo, ii.  to carry out research at the educational institution or facility or at the zoo, iii.  in the case of fish cultured in an aquarium at an educational institution or facility other than a post-secondary institution, to raise the fish in a classroom for stocking purposes as part of a program to assist in the conservation of that species in Ontario. 4.  Subject to subsection (1.1), the person cultures fish in an aquarium located at premises that are generally open to the public for the sole purpose of exhibiting the fish to the public at the premises. O. Reg. 324/13, s. 2 (1); O. Reg. 759/20, s. 3 (1). (1.1) A person who engages in aquaculture in the circumstances described in paragraph 3 or 4 of subsection (1) is not exempt from subsection 47 (1) of the Act unless the following conditions are met: 1.  Subject to paragraph 2, all wastewater and effluent from the aquarium must be, i.  discharged into a municipal wastewater treatment system, or ii.  disinfected by a process that removes or inactivates pathogens such as viruses, bacteria and protozoa in water. 2.  In the case of fish cultured in an aquarium for the purposes of carrying on research under subparagraph 3 ii of subsection (1), all wastewater and effluent from the aquarium must be disinfected by a process that removes or inactivates pathogens such as viruses, bacteria and protozoa in water. 3.  Any fish that are cultured in the aquarium and subsequently die must not be deposited in natural waters. 4.  Any fish used in research must be humanely euthanized upon the completion of the research unless the fish will be used for display or educational purposes at the educational institution or facility or at the zoo at which the research was carried out. O. Reg. 324/13, s. 2 (1); O. Reg. 759/20, s. 3 (2). (2) Subsection 51 (1) of the Act does not apply to the buying or selling of goldfish, koi (Cyprinus carpio) and the species of tilapia set out in Schedule B if these fish are bought or sold for the purpose of the aquarium trade.  O. Reg. 113/04, s. 1. (3) In this section, “aquarium” means an artificial container used to culture fish with no inflow or outflow directly connected to natural waters; (“aquarium”) “aquarium trade” means the culturing, buying or selling of aquarium, tropical or ornamental fish for the pet or hobby market; (“aquariophilie”) “educational institution or facility” means a daycare, nursery school, primary school, high school, private school or post-secondary institution. (“établissement d’enseignement”) “research” means the use of fish in connection with studies, investigation and teaching in any field of knowledge, and without limiting the generality of the foregoing, includes the use of fish for the performance of tests, and diagnosis of disease and the production and testing of preparations intended for use in the diagnosis, prevention and treatment of any disease or condition; («travaux de recherche») “zoo” means a place that is an accredited member of Canada’s Accredited Zoos and Aquariums and at which fish are kept in captivity for display to the public and for conservation, educational or scientific purposes. («zoo») O. Reg. 664/98, s. 24 (3); O. Reg. 324/13, s. 2 (2, 3); O. Reg. 759/20, s. 3 (3). Fish Stocking Licences