28. (1) Where a discharger is required to perform a seven-day fathead minnow growth inhibition test, the discharger shall perform the test according to the procedure described in the Environment Canada publication entitled “Biological Test Method: Test of Larval Growth and Survival Using Fathead Minnows”, as amended from time to time.  O. Reg. 234/07, s. 7. (2) Where a discharger is required to perform a seven-day Ceriodaphnia dubia reproduction inhibition and survivability test, the discharger shall perform the test according to the procedure described in the Environment Canada publication entitled “Biological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia”, as amended from time to time.  O. Reg. 234/07, s. 7. (3) On one day in each semi-annual period, on a day on which samples are picked up at the plant under subsection 23 (1), each discharger shall collect and immediately pick up a grab sample from each process effluent sampling point at the discharger’s plant, and shall perform a seven-day fathead minnow growth inhibition test and a seven-day
Ceriodaphnia dubia reproduction inhibition and survivability test on each sample.  O. Reg. 560/94, s. 28 (3). (4) There shall be an interval of at least 90 days between successive pick-up days at the plant under subsection (3).  O. Reg. 560/94, s. 28 (4). (5) All samples picked up under subsection (3) in a semi-annual period shall be picked up on the same day in the semi-annual period.  O. Reg. 560/94, s. 28 (5). (6) A discharger need not collect a sample from a sampling point in accordance with subsection (3) until 12 consecutive monthly rainbow trout acute lethality tests and 12 consecutive monthly Daphnia magna acute lethality tests performed on samples collected at the sampling point at a discharger’s plant result in mortality for no more than 50 per cent of the test organisms in 100 per cent effluent.  O. Reg. 560/94, s. 28 (6). Monitoring — Cooling Water Effluent — Weekly Assessment

29. (1) Each discharger shall, on one day in each week, pick up a set of samples collected at each cooling water effluent sampling point at the discharger’s plant and shall analyze each set of samples for each Schedule 2 assessment parameter. (2) There shall be an interval of at least four days between successive pick-up days at the plant under subsection (1). (3) All samples picked up under subsection (1) in a week shall be picked up on the same day in the week.  O. Reg. 560/94, s. 29. Monitoring — Overflow Effluent — Assessment

30. (1) Each discharger shall, during each eight-hour period in which overflow effluent is discharged from the plant, collect a grab sample at each overflow effluent sampling point at the discharger’s plant and shall analyze each sample for each Schedule 3 assessment parameter. (2) Each grab sample collected under subsection (1) shall be picked up within four hours of when it was collected.  O. Reg. 560/94, s. 30. PART VI   EFFLUENT VOLUME Flow Measurement

31. (1) Subject to subsection (7), for the purposes of this section, a volume of effluent for a stream for a day is the volume that flowed past the sampling point established under Part II on the stream during the 24-hour period preceding the pick-up of the first sample picked up from the stream for the day. (2) Each discharger shall determine in cubic metres a daily volume of effluent for each process effluent stream at the discharger’s plant for each day on which a sample is collected under this Regulation from the stream. (3) Each discharger shall use flow measurement methods that allow the daily volumes for process effluent streams to be determined to an accuracy of within plus or minus 15 per cent. (4) Each discharger shall determine in cubic metres a daily volume of effluent for each cooling water effluent stream at the discharger’s plant for each day on which a sample is collected under this Regulation from the stream. (5) Each discharger shall use flow measurement methods that allow the daily volumes for cooling water effluent streams to be determined to an accuracy of within plus or minus 20 per cent. (6) Each discharger shall determine in cubic metres a volume of effluent for each overflow effluent monitoring stream at the discharger’s plant for each eight-hour period for which a sample is collected under this Regulation from the stream. (7) For the purposes of subsection (6), a volume of effluent for an overflow effluent monitoring stream is the volume that flowed past the overflow effluent sampling point on the stream during the eight-hour period. (8) Each discharger shall use flow measurement methods that allow the volumes for overflow effluent streams to be determined to an accuracy of within plus or minus 20 per cent. (9) Each discharger shall, no later than the day that is 90 days after the date on which this Regulation begins to apply to the plant, determine by calibration or confirm by means of a certified report of a registered professional engineer of the Province of Ontario that each flow measurement method used under subsection (2) meets the accuracy requirements of subsection (3), that each flow measurement method used under subsection (4) meets the accuracy requirements of subsection (5), and that each flow measurement method used under subsection (6) meets the accuracy requirements of subsection (8). (10) Where a discharger uses a new flow measurement method or alters an existing flow measurement method, the discharger shall determine by calibration or confirm by means of a certified report of a registered professional engineer of the Province of Ontario that each new or altered flow measurement method meets the accuracy requirements of subsection (3), (5) or (8), as the case may be, within two weeks after the day on which the new or altered method or system is used. (11) Each discharger shall develop and implement a maintenance schedule and a calibration schedule for each flow measurement system installed at the discharger’s plant and shall maintain each flow measurement system according to good operating practices. (12) Each discharger shall use reasonable efforts to set up each flow measurement system used for the purposes of this section in a way that permits inspection by a provincial officer.  O. Reg. 560/94, s. 31. Calculation of Plant Volumes

32. (1) Each discharger shall calculate, in cubic metres, a daily process effluent plant volume for each day. (2) For the purposes of subsection (1), a process effluent plant volume for a day is the sum of the daily process effluent volumes determined under section 31 for the day. (3) Each discharger shall calculate, in cubic metres, a monthly average process effluent plant volume for each month, by taking the arithmetic mean of the daily process effluent plant volumes calculated under subsection (1) for the month. (4) Each discharger shall calculate, in cubic metres, a daily cooling water effluent plant volume for each day. (5) For the purposes of subsection (4), a cooling water effluent plant volume for a day is the sum of the daily cooling water volumes determined under section 31 for the day. (6) Each discharger shall calculate, in cubic metres, a monthly average cooling water effluent plant volume for each month, by taking the arithmetic mean of the daily cooling water effluent plant volumes calculated under subsection (4) for the month.  O. Reg. 560/94, s. 32. PART VII   STORM WATER CONTROL STUDY Storm Water Control Study