26. (1) Where a discharger is required to perform a seven-day fathead minnow growth inhibition test, the discharger shall perform the test according to the procedure described in the Environment Canada publication entitled “Biological Test Method: Test of Larval Growth and Survival Using Fathead Minnows”, as amended from time to time.  O. Reg. 240/07, s. 6. (2) Where a discharger is required to perform a seven-day Ceriodaphnia dubia
reproduction inhibition and survivability test, the discharger shall perform the test according to the procedure described in the Environment Canada publication entitled “Biological Test Method: Test of Reproduction and Survival Using the Cladoceran Ceriodaphnia dubia”, as amended from time to time.  O. Reg. 240/07, s. 6. (3) On one day in each semi-annual period, on a day on which samples are picked up at the plant under subsection 20 (1), each discharger shall collect and immediately pick up a grab sample from each non-event process effluent sampling point at the discharger’s plant and shall perform a seven-day fathead minnow growth inhibition test and a seven-day Ceriodaphnia dubia reproduction inhibition and survivability test on each sample.  O. Reg. 215/95, s. 26 (3). (4) On one day in each semi-annual period, on a day on which samples are picked up at the plant for analysis under subsection 21 (12), each discharger shall, (a) collect and immediately pick up a grab sample from the event process effluent stream of the type described in paragraph 2 of subsection 1 (3) at the discharger’s plant; and (b) perform a seven-day fathead minnow growth inhibition test and a seven-day Ceriodaphnia dubia
reproduction inhibition and survivability test on the sample.  O. Reg. 215/95, s. 26 (4). (5) Instead of collecting a grab sample from the sampling point on the stream as required by subsection (4), a discharger may collect a grab sample from a radioactive liquid waste management system tank at the plant immediately before a discharge into the stream.  O. Reg. 215/95, s. 26 (5). (6) There shall be an interval of at least 90 days between successive pick up days at the plant under subsections (3) and (4).  O. Reg. 215/95, s. 26 (6). (7) All samples picked up under subsections (3) and (4) in a semi-annual period shall be picked up on the same day in the semi-annual period.  O. Reg. 215/95, s. 26 (7). (8) A discharger need not collect a sample from a sampling point in accordance with subsections (3) and (4) until 12 consecutive monthly rainbow trout acute lethality tests and 12 consecutive monthly Daphnia magna acute lethality tests performed on samples collected at the sampling point at a discharger’s plant result in mortality for no more than 50 per cent of the test organisms in 100 per cent effluent.  O. Reg. 215/95, s. 26 (8). Monitoring — Building Effluent — Quarterly

27. (1) Each discharger shall, on one day in each quarter, pick up a grab sample at each building effluent sampling point at the discharger’s plant and shall analyze each sample for the following parameters: 1. Total Suspended Solids (TSS), referred to as Analytical Test Group 8 in the Ministry of the Environment publication entitled “Protocol for the Sampling and Analysis of Industrial/Municipal Wastewater”, as amended from time to time. 2. Oil and grease, referred to in Analytical Test Group 25 in the Ministry of the Environment publication entitled “Protocol for the Sampling and Analysis of Industrial/Municipal Wastewater”, as amended from time to time.  O. Reg. 215/95, s. 27 (1); O. Reg. 240/07, s. 7. (2) There shall be an interval of at least 45 days between successive pick up days at the plant under subsection (1).  O. Reg. 215/95, s. 27 (2). PART VI   EFFLUENT VOLUME Flow Measurement