jimnoneill/paper-to-field-training huggingface dataset:
Rapid and Simple Procedure for Homogenizing Leaf Tissues Suitable for Mini‐Midi‐Scale DNA Extraction in Rice

We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3+/-1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 microg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.

Topic: Plant Disease Resistance and Genetics
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Determinants of Foreign Direct Investment in services sector of Pakistan: An econometrics approach

This study examines the key determinants of FDI inflows in Services sector of Pakistan, using time series data (quarterly) for the period of 1996Q1-2008Q4. To check the stationarity of the data, ADF test has been applied. Co-integration and Error Correction Model (ECM) is used for estimation of the data. This study found that Gross Domestic Fixed Capital formation (GDFCF), Inflation Rate(INF), Current Account Balance(CAB), Exchange Rate (ER), and Per Capita Income (PC) are main factors of FDI inflows in services sector of Pakistan. This study reveals that GDFCF, INF and PC are found statistically significant with positive signs. CAB and ER are also statistically significant with negative sign. While, trade openness (TO) is found statistically insignificant but with negative sign.

Topic: Global Financial Crisis and Policies
Subfield: Finance
Field: Economics, Econometrics and Finance
Domain: Social Sciences
Evaluation of cotton polyphenols as factors of resistance to root-knot nematode and Fusarium wilt

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvaluation of cotton polyphenols as factors of resistance to root-knot nematode and Fusarium wiltPaul A. Hedin, Raymond L. Shepherd, and A. J. Kappelman Jr.Cite this: J. Agric. Food Chem. 1984, 32, 3, 633–638Publication Date (Print):May 1, 1984Publication History Published online1 May 2002Published inissue 1 May 1984https://pubs.acs.org/doi/10.1021/jf00123a052https://doi.org/10.1021/jf00123a052research-articleACS PublicationsRequest reuse permissionsArticle Views99Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

Topic: Plant Disease Management Techniques
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Teeth in a day. The Branemark Novum system.

The Branemark Novum system is a one-day treatment of the edentulous or periodontally hopeless mandibular dentition. There are four drill templates and eight drill guides that precisely position three implants which are totally parallel and level. A prefabricated lower bar is placed on the three implants, and an upper bar fits on the lower bar. The restorative dentist has previously selected denture teeth and recorded the vertical dimension of occlusion. The case is waxed up, adjusted, processed and delivered in one day.

Topic: Dental Implant Techniques and Outcomes
Subfield: Oral Surgery
Field: Dentistry
Domain: Health Sciences
Data from A Phase Ib/II Study of Pepinemab in Combination with Avelumab in Advanced Non–Small Cell Lung Cancer

&lt;div&gt;AbstractPurpose:&lt;p&gt;The CLASSICAL-Lung clinical trial tested the combination of pepinemab, an IgG4 humanized mAb targeting semaphorin 4D, with the PD-L1 inhibitor avelumab to assess the effects of coupling increased T-cell infiltration and reversal of immune suppression via pepinemab with sustained T-cell activation via checkpoint inhibition.&lt;/p&gt;Patients and Methods:&lt;p&gt;This phase Ib/II, single-arm study was designed to evaluate the safety, tolerability, and efficacy of pepinemab in combination with avelumab in 62 patients with advanced non–small cell lung cancer (NSCLC), including immunotherapy-naïve (ION) patients and patients whose tumors progressed following anti-PD-1/L1 monotherapy (IOF). The main objectives were to evaluate safety/tolerability, establish a recommended phase 2 dose (RP2D), obtain a preliminary evaluation of antitumor activity, and investigate candidate biomarker activity.&lt;/p&gt;Results:&lt;p&gt;The combination was well tolerated with no major safety signals identified. Pepinemab, 10 mg/kg with avelumab, 10 mg/kg, every 2 weeks, was selected as the RP2D. Among 21 evaluable ION patients, 5 patients experienced partial responses (PR), 4 patients evidenced clinical benefit ≥1 year, and the disease control rate (DCR) was 81%. Notably, overall response rate with the combination therapy was higher than previously reported for single-agent avelumab in the PD-L1-negative/low population. Among 29 evaluable IOF patients, the combination resulted in a DCR of 59%, including 2 PR and 7 patients with durable clinical benefit of ≥23 weeks. Biomarker analysis of biopsies demonstrated increased CD8 T-cell density correlating with RECIST response criteria.&lt;/p&gt;Conclusions:&lt;p&gt;The combination of pepinemab with avelumab was well tolerated in NSCLC and showed signs of antitumor activity in immunotherapy-resistant and PD-L1-negative/low tumors.&lt;/p&gt;&lt;/div&gt;

Topic: Toxin Mechanisms and Immunotoxins
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Applications of Multidimensional Scaling to Research in the Health Professions

Multidimensional scaling (MDS) is a procedure for transforming similarity data such as correlations into a graph or map. Although MDS is similar to factor analysis in function and purpose, it is more flexible and often produces results that are easier to interpret. This article discusses MDS and its potential use for research and evaluation in the health-related professions. The first half of the article presents an overview of MDS, the types of data that can be used as input, and issues related to interpretation of results. The second half describes various applications of MDS, including the development of a working taxonomy of nursing specialty practice, the identification of the dimensions underlying physician performance, and the classification of different types of psychotherapies examined in the metaanalysisperformedby Smith and Glass (1977). The article concludes with a brief discussion on how MDS relates to factor analysis, discriminant analysis, and cluster analysis.

Topic: Community Health and Development
Subfield: General Health Professions
Field: Health Professions
Domain: Health Sciences
Magnetic-field effect on the photoactivation reaction of <i>Escherichia coli</i> DNA photolyase

One of the two principal hypotheses put forward to explain the primary magnetoreception event underlying the magnetic compass sense of migratory birds is based on a magnetically sensitive chemical reaction. It has been proposed that a spin-correlated radical pair is produced photochemically in a cryptochrome and that the rates and yields of the subsequent chemical reactions depend on the orientation of the protein in the Earth's magnetic field. The suitability of cryptochrome for this purpose has been argued, in part, by analogy with DNA photolyase, although no effects of applied magnetic fields have yet been reported for any member of the cryptochrome/photolyase family. Here, we demonstrate a magnetic-field effect on the photochemical yield of a flavin–tryptophan radical pair in Escherichia coli photolyase. This result provides a proof of principle that photolyases, and most likely by extension also cryptochromes, have the fundamental properties needed to form the basis of a magnetic compass.

Topic: Photoreceptor and optogenetics research
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Studies on the biochemical mode of action of EGYT-475, a new antidepressant.

We studied the mode of action of N-benzyl-piperazine-picolinylfumarate (EGYT-475) and of its metabolite N-benzyl-piperazine (EGYT-2760) in CFY rats. It was found that EGYT-475 had no uptake-inhibitory effect but EGYT-2760 inhibited the high-affinity uptake of 3H-noradrenaline, 3H-dopamine and especially that of 3H-serotonin both in vitro and ex vivo. Neither of the two compounds changed the serotonin turnover. Only EGYT-2760 evoked hyperthermia in rats at a high ambient temperature (28 degrees C). This effect was abolished by cyproheptadine but not by amitriptyline. EGYT-2760 antagonized serotonin-induced contractions of the stomach fundus but it was inactive in inhibiting the serotonin-induced platelet aggregation. Our results suggest that EGYT-2760, an active metabolite of EGYT-475, has a central serotoninomimetic action which involves 5-HT uptake-inhibition and 5-HT1 receptor agonistic effect.

Topic: Neurotransmitter Receptor Influence on Behavior
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Millerigobius_J1735.tifX-ray data set

X-ray data set used in "Skeletal traits and otoliths can unravel the relationships within European Gobiidae (Gobius lineage sensu lato)" Bettina Reichenbacher, Jasna Vukić, Radek Šanda, Ulrich K. Schliewen, Hamid R. Esmaeili, Abderrahmane Kassar, IN: Zoological Journal of the Linnean Society, 2023, XX, 1–32 https://doi.org/10.1093/zoolinnean/zlad058 <br> scale bar of X-ray images = 1 cm

Topic: Geomagnetism and Paleomagnetism Studies
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Diagnosis of the propensity of the driver to dangerous (aggressive) driving

The article is devoted to the study of the phenomenon of &amp;#34;dangerous driving&amp;#34;, as well as the assessment of the propensity to aggressive management style. Diagnostic tools made personal questionnaire assessing the reliability of the driver. Based on the study characteristics such as discipline, self-control, responsibility, aggressiveness, risk tolerance, desire for self-assertion, energy, arrogance, anxiety is projected the style of the vehicle control (Style). Empirically it is proved that the indicator of the Style allows to statistically significantly difference between drivers that differ reliable style of driving, and drivers with dangerous style of driving. The proposed approach can be applied in the framework of professional psychological selection of professionals to the professions of risk, and the admission of drivers to management of special vehicle. The widespread use of the technique is in driving with individualization of training and the correction style control vehicle.

Topic: Technology and Human Factors in Education and Health
Subfield: Public Health, Environmental and Occupational Health
Field: Medicine
Domain: Health Sciences
Comment on egusphere-2022-501

<strong class="journal-contentHeaderColor">Abstract.</strong> The specific surface area (SSA) of snow is directly measured by X-ray computed tomography or indirectly using the reflectance of near-infrared light. The IceCube is a well-established spectroscopic instrument using a near-infrared wavelength of 1310 nm. We compared the SSA of six snow types measured with both instruments. The IceCube measured significantly higher values with a relative percentage difference between 20 to 52 % for snow types with an SSA between 5 to 25 m<sup>2</sup> kg<sup>&minus;1</sup>. There is no significant difference for snow with an SSA between 30 to 80 m<sup>2</sup> kg<sup>&minus;1</sup>. The difference is statistically significant between snow types but not uniquely related to the SSA. We suspected that artificially created particles were the source of the difference. These were sampled, measured and counted. Numerical simulations with radiation transfer solver TARTES confirm the observation.

Topic: Cryospheric studies and observations
Subfield: Atmospheric Science
Field: Earth and Planetary Sciences
Domain: Physical Sciences
Investigation of somatic variants associated with seed content in grapevine

Two different mechanisms are involved in grape seedlessness, namely parthenocarpy and stenospermocarpy. In parthenocarpic conditions fruit usually develops from the ovary in the absence of fertilization yielding small berries that completely lack seeds, whereas in stenospermocarpy pollination and fertilization take place normally, but seed development aborts at an early stage after fertilization and berry size at harvest is reduced. Most cultivated seedless grapes exhibit the Sultanina-derived stenospermocarpy whereas different sources of seedlessness have been much less investigated and exploited.&#13;\nHere we report the characterization of several somatic variants for seed content discovered in the grapevine germplasm collections at FEM and CNR-IPSP. These accessions were evaluated for fruit and seed set in open-pollinated, self-pollinated, and emasculated conditions.&#13;\nSingle nucleotide polymorphism (SNP) candidates between seeded and seedless mutants were identified by using the Vitis20K SNP array and RNA-Seq-based variant calling. The latter was applied to the transcriptome comparison between Sangiovese and Corinto Nero cultivars previously reported by Nwafor et al (2014).

Topic: Hermeneutics and Narrative Identity
Subfield: Philosophy
Field: Arts and Humanities
Domain: Social Sciences
The efficacy of some pour-on insecticides for the control of long-nosed sucking lice<i>(Linognathus vituli)</i>on cattle

Sheet music for "Does it Pay to Raise Your Boy to be a Solider?," a patriotic, military readiness song published before the United States entered the First World War. The story of the song is told through "tearjerker lyrics" of a son lost to war and a grieving mother. The cover features a facsimile of a letter from Theodore Roosevelt commenting on the poem that comprises the song's lyrics.

Topic: Insect and Pesticide Research
Subfield: Insect Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Identification of Chromosomal Regions and Candidate Genes for Round leaf Locus in Cucumis melo L.

Leaf morphology plays a crucial role in plant classification and provides a significant model for studying plant diversity while directly impacting photosynthetic efficiency. In the case of melons, leaf shape not only influences production and classification but also represents a key genetic trait that requires further exploration. In this study, we utilized forward genetics to pinpoint a recessive locus, dubbed Cmrl (Round leaf), which is responsible for regulating melon leaf shape. Through bulked segregant analysis sequencing and extensive evaluation of a two-year F2 population, we successfully mapped the Cmrl locus to a 537.07 kb region on chromosome 8 of the melon genome. Subsequent genetic fine-mapping efforts, leveraging a larger F2 population encompassing 1322 plants and incorporating F2:3 phenotypic data, further refined the locus to an 80.27 kb interval housing five candidate genes. Promoter analysis and coding sequence cloning confirmed that one of these candidates, MELO3C019152.2 (Cmppr encoding a pentatricopeptide repeat-containing family protein, Cmppr), stands out as a strong candidate gene for the Cmrl locus. Notably, comparisons of Cmrl expressions across various stages of leaf development and different leaf regions suggest a pivotal role of Cmrl in the morphogenesis of melon leaves.

Topic: Advances in Cucurbitaceae Research
Subfield: Genetics
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
ESSENTIALISM AND RIGIDITY

Michael Della Rocca has recently argued that Kripkean essentialism is subtly self‐defeating: to defend it, certain modal intuitions must be reconstrued in terms of similarity, but reconstruing them in this way threatens the principled rejection of similarity comparisons on which Kripke's essentialism depends. Della Rocca holds that Kripke's strategy must assume the necessity of identity, and that the necessity of identity already presupposes essentialism, which renders the defence circular. Against this, I argue that the necessity of identity may be accepted independently; therefore no circularity need arise. I also argue that Della Rocca fails to rebut an objection raised by Stephen Yablo.

Topic: Philosophy and Theoretical Science
Subfield: Experimental and Cognitive Psychology
Field: Psychology
Domain: Social Sciences
Market chain analysis of teff and wheat production in Halaba Special Woreda, southern Ethiopia

This research attempted to analyze the market chain of teff and wheat in Halaba with specific objective of assessing the structure-conduct-performance of teff and wheat marketing, the various marketing channels, the institutional support services of extension, input supply, and credit and analyzing the determinants of teff and wheat supply. Primary data were collected from160 teff and wheat producers and 43 grain traders based on two stage random sampling method. Multiple linear regression model was employed to estimate the determinants of teff and wheat supply. The results of the study indicated that out of the total teff and wheat produced by sample farmers, 86.2% of teff and 49.2% of wheat were marketed. Urban assemblers, regional wholesalers and regional retailers bought 40%, 37.4% and 16.5% the teff marketed respectively. Likewise, wholesalers and urban assemblers bought 45.1% and 43.8% of wheat marketed respectively. Alaba Qulito sample market was inefficient, characterized by oligopolistic market structure. The major barrier to enter into the market was shortage of capital. Licensing and years of trading experience did not hinder entry into teff and wheat trading activities. Moreover, the markets were overwhelmed by information asymmetry with low degree of market transparency. Although trading of teff and wheat is profitable across all sample farmers and traders, problems like oligopolistic market structure and information asymmetry made the trading business uncompetitive and inefficient. Among the different variables hypothesized to determine the supply of teff and wheat, econometric result showed that four variables such as quantity produced, access to market information, access to extension service and sex of the household head significantly affected the volume of teff supplied to the market. Moreover, three variables namely quantity produced, access to credit and price of other (pepper) crop significantly affected volume of teff supplied to the market. As hypothesised, all variables took a sign as expected. The study recommends providing policies that improve teff and wheat production capacity by identifying new technologies create stable demand for surplus production would enhance farmers’ decision in marketable surplus. Strengthening Institutions that convey reliable and timely market xv information required by all market participant. Strengthening the existing extension system through training in a way to serve grassroots level producers in all aspect is important. The number of farmers and traders who accessed credit is very limited; therefore, financial institutions should design a mechanism to address the challenges of financial access to smallholder farmers and traders. Eventually, policies that strengthen the bargaining power of cooperative are vital in order to reduce the market inefficiency created due to oligopolistic nature of market structure.

Topic: Agriculture and Rural Development Research
Subfield: Ecology, Evolution, Behavior and Systematics
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Age of menarche in girls with cystic fibrosis.

Malnutrition, delayed growth and puberty are commonly observed in children suffering from cystic fibrosis. The aim of this study was to assess the age of menarche in girls with CF using status quo analysis. The relationship between types of CFTR mutations and onset of the first menstruation was also evaluated. Material was based on somatic data gathered from medical history records of 47 girls with cystic fibrosis, aged 11-18 years. All girls were patients of the Mother and Child Institute in Warsaw (Poland). Results: The age of menarche in the girls in the study group was 14.65+/-1.21 years. In comparison with the healthy child population, girls with cystic fibrosis experienced menarche with 2 years' delay. Menstruating girls were found to be statistically older and taller than their non-menstruating consorts. Regarding body mass and BMI, a marked tendency towards higher parameter values was noted in the menstruating group, although the differences did not reach statistical significance. A significant relationship between onset of menarche and type of CFTR mutation was found. Girls with cystic fibrosis enter puberty later than their peers, in spite of intensive medical care. The issue of growth and puberty in children with CF requires further detailed investigation under clinical and auxological aspects.

Topic: Cystic Fibrosis Research Advances
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
agent vosaroxin

Purpose: Vosaroxin is a first in class naphthyridine analog structurally related to quinolone antibacterials, that intercalates DNA and inhibits topoisomerase II. Vosaroxin is not a P-glycoprotein receptor substrate and its activity is independent of p53, thus evading common drug resistance mechanisms. To evaluate vosaroxin as a clinically applicable radiation sensitizer, we investigated its effects on tumor cell radiosensitivity in vitro and in vivo. Methods: Vosaroxin’s effect on post-irradiation sensitivity of U251, DU145, and MiaPaca-2 cells was assessed by clonogenic assay. Subsequent mechanistic and in vivo studies were performed with U251 cells. Cell cycle distribution and G2 checkpoint integrity was analyzed by flow cytometry. DNA damage and repair was evaluated by a high throughput gamma-H2AX assay. Apoptosis was assessed by flow cytometry. Mitotic catastrophe was assessed by microscopic evidence of fragmented nuclei by immunofluorescence. In vivo radiosensitization was measured by subcutaneous tumor growth delay. Results: 50-100 nmol/L treatment with vosaroxin resulted in radiosensitization of all 3 cell lines tested with a dose enhancement factor of 1.20 to 1.51 measured at a surviving fraction of 0.1. The maximal dose enhancement was seen in U251 cells treated with 75 nmol/L vosaroxin (DEF 1.51). Vosaroxin exposure did not change cell cycle distribution prior to irradiation nor alter G2 checkpoint integrity after irradiation. No difference was seen in the

Topic: Cancer therapeutics and mechanisms
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences