jimnoneill/paper-to-field-training huggingface dataset:
Quantitative Analysis of Gene Expression During Calcium Homeostasis in E. coli

Despite a wealth of knowledge regarding calcium’s role as secondary messenger in eukaryotic cells, relatively little is known about calcium homeostasis in bacteria. Our study employing molecular genetics and transcriptome analysis of calcium homeostasis previously exhibited a calcium regulation of gene expression in elevated or depleted calcium throughout E. coli genome. Here we report the results of our subsequent investigation into quantitative analysis using quantitative reverse transcriptase PCR (qRT-PCR) of gene expression in subsets of genes from wild-type and mutant strains with addition of calcium and chelation of calcium. Data from qRT-PCR reveal very high upregulation of genes yjeE and secA indicating their possible role as overactive calcium efflux systems in E. coli. Calcium is highly likely to be involved in stress response as evidenced by upregulation of marB in transposon mutants and by 10-fold induction of rpoS in elevated calcium condition in mutants defective in calcium transport.&#x0D; Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 47-54

Topic: Bacterial Genetics and Biotechnology
Subfield: Genetics
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
A Computer Program Package For Use With the Southern Pine Seed Orchard Inventory-Monitoring System

Southern pine seed orchards covering more than 10,000 acres currently produce over 160,000 pounds of improved seed having a potential of 1 billion seedlings annually. By the year 2000, annual seed production · is expected to reach 500,000 pounds. Seed orchards not only represent potential for improved growth, wood quality and pest resistance, but they also represent a large capital investment in orchard establishment and equipment and the ~ignificant annual costs of orchard maintenance, protection and harvesting. Since the immediate goal of the seed orchard investment is the annual production of cones and seed, the task of the seed orchard manager could be greatly lightened by a system that will forecast annual cone and seed crops and monitor production efficiency. Such a system has been developed by Bramlett and Godbee (1982). In the Inventory- Moni taring System (IMS) a set of sample trees are chosen from the seed orchard population. Then, based on the survival of cones on tagged sample branches in each sample tree, the expected number of cones and seed from the orchard can be predicted as early as 18 months prior to cone harvest. Bramlett and Godbee (1982) detailed various procedures for the selection of sample trees as well as methods for choosing sample branches and conducting flower, cone let and cone counts. Besides providing guidelines for data collection in the orchard, the authors also defined the variables used in the calculation of predicted cone and seed yields, including cone efficiency, seed potential, seed efficiency, extraction efficiency and germination efficiency. They showed how to compute (or update) the values of these variables and how to apply them in models to calculate predicted bushels of cones, predicted pounds of seed, predicted number of seedlings and other predicted values for the orchard. In addition to these predicted values, Bramlett and Godbee (1982) also demonstrated how the IMS can be used by the orchard manager to evaluate orchard productivity, identify the factors reducing yields, and formulate corrective action, including fertilization and pest management. \nBecause a great deal of record-keeping and repetitive mathematical operations are involved in the IMS, it is ideally suited to computerization. A computerized version of the IMS not only has the advantage of efficient data storage and manipulation, but it also makes possible the application of more sophisticated mathematical models as they become available, and facilitates the utilization of productivity data accumulated from year to year to improve the accuracy\nof the system.

Topic: Plant Physiology and Cultivation Studies
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
虫草保健品中13种核苷类成分的UHPLC-ESI-MS/MS定量分析及评价

目的:对香港和大陆市场上销售的8种常见虫草保健品进行核苷类成分的定量分析及评价。方法:采用冰浴超声提取法与UHPLC-ESI-MS/MS分析法, 快速检测虫草保健品中的13种核苷类成分。结果:虫草保健品中被测核苷类成分的含量明显高于天然冬虫夏草药材。这些保健品绝大部分组成为冬虫夏草菌丝体粉, 而并非是由天然冬虫夏草经直接粉碎加工而成。结论:通过对13种核苷类成分的含量测定, 比较市场上以虫草药材或虫草菌丝体为原料制备的保健品质量, 可以反映部分已经上市销售的虫草类保健品在核苷类成分种类及含量之间的差异性。Objective: To investigte the nucleoside compounds from eight kinds of common Cordyceps health care products which were sold in Hongkong and mainland markets. Methods: A combination of ultrasonic extraction in an ice bath and UHPLC-ESI-MS / MS analysis method were used for fast simultaneous determination of 13 nucleosides in the health care products of Cordyceps. Results: The content of targeted nucleoside compounds in health care products were significantly higher than natural medicinal materials of Cordyceps sinensis. The components of health care products mostly were Cordyceps sinensis mycelium powder, rather than from the natural medicinal materials of Cordyceps sinensis by crushing and processing directly. Conclusion: To determine the nucleosides components in Cordyceps, and compared the quality of health care products which were made from the medicinal materials and mycelium of Cordyceps sinensis in the market, which results may reflect the differences between nucleosides types and contents which have sold in the market of Cordyceps health care products.

Topic: Fungal Biology and Applications
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Co-creating Norwegian DigiPer: a digital tool including peer support for managing mental, physical, and social health in serious mental illness

<title>Abstract</title> Background People with serious mental illness (SMI) often encounter physical health issues, a lack of focus on self-management, and persistent symptoms of mental illness. Strategies to empower people with SMI to self-manage their mental, physical, and social health are needed. The PeerTECH application is an American digital peer support intervention that can help manage health issues and promote self-management skills for people with SMI. This study aims to co-create a Norwegian version of PeerTECH, DigiPer, with service users, peer support workers, and professionals from Norwegian community mental healthcare. Methods This study employs a qualitative design using principles of co-creation to develop the Norwegian DigiPer. We conducted workshops, videoconference demonstrations, and application testing with key stakeholders in the co-creation process. The data collected were analyzed using thematic analysis. Results Five themes and ten subthemes were developed in the data analysis. The first theme reflected feedback and challenges in DigiPer functioning, with the subthemes of <italic>Usability in DigiPer</italic> and <italic>Managing complexity in DigiPer classes.</italic> The second theme focused on the peer support worker's role, with subthemes of <italic>recognizing and addressing user challenges</italic> and <italic>balancing self-responsibility and support.</italic> The third theme focused on the content of DigiPer in addressing day-to-day challenges, with subthemes of <italic>supporting personal growth and goal setting</italic> and <italic>DigiPer as a tool to build supportive networks</italic>. The fourth theme highlighted the use of DigiPer to encourage a healthy lifestyle, with subthemes of <italic>addressing substance use and smoking habits</italic> and <italic>improving sleep and stress management.</italic> The fifth theme provided a holistic view of health, with subthemes of <italic>connecting users to relevant services</italic> and <italic>a need for support to overcome financial challenges.</italic> Conclusions DigiPer has good usability among community mental healthcare stakeholders and has proper functionality, which is relevant to the Norwegian context. Although challenges exist, all participants perceived DigiPer as a meaningful and promising tool to help people with SMI improve their self-management of their mental, physical, and social health. Future studies should assess the accessibility and feasibility of the Norwegian DigiPer.

Topic: Mental Health and Patient Involvement
Subfield: General Health Professions
Field: Health Professions
Domain: Health Sciences
Time- and dose-dependent differential upregulation of three genes by 17β-estradiol in endothelial cells

The purpose of this study was to identify genetic targets in the vasculature for estrogen by profiling genes expressed in female human aortic endothelial cells exposed to various doses of 17β-estradiol at differing concentrations and for differing periods of time. Our approach employed a RT-PCR-based cloning strategy of DNA differential display analysis, with differential expression verified by semiquantitative PCR performed with gene-specific primers. A significant increase in mRNA expression in response to 17β-estradiol was observed for the following three genes: aldose reductase (3.4-fold), caspase homologue-α protein (4.2-fold), and plasminogen activator inhibitor-1 intron e (2.3-fold). For all three upregulated genes, estradiol-induced upregulation occurred with a similar time course and temporally clustered to the first 24 h after hormone treatment. In addition, the effect of estradiol dose on gene expression was consistent and occurred at physiological concentrations. Our results describe previously uncharacterized estradiol-sensitive time- and dose-dependent regulation of genes with potential importance to vascular function in human endothelial cells.

Topic: Estrogen and related hormone effects
Subfield: Genetics
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Plasma Catecholamines Assay: Comparison Between Fluorimetric and Radioenzymatic Methods

Plasma catecholamines were simultaneously measured in duplicate plasma samples by the fluorimetric method of Renzini et al. (1970) Clin. Chim. Acta 39, 587-594) and by the radioenzymatic method of Da Prada & Zurcher ((1976) Life Sci. 19, 1161-1174). The correlation of noradrenaline and adrenaline plasma concentrations determined by the two methods were, respectively, r = 0.95 (p less than 0.001) and r = 0.75 (p less than 0.01). The fluorimetric method was less sensitive, but more economical and less time-consuming than the radioenzymatic method. The fluorimetric method is still of value for measuring plasma catecholamines in man.

Topic: Ion channel regulation and function
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Development of Career Development Inventory for Elementary student

일자리의 생성·소멸을 동반하는 직업은 변화된 기술환경 속에서 새롭게 생겨나기도 하고, 때로는 사라지는 경우도 있다. 초등학교 진로교육이 시대변화에 따른 보조를 맞 추기 위해서는 최신 산업기술의 발달로 인하여 생겨난 직업용어, 학교진로교육 목표를 반영한 검사도구가 마련되어야 한다. 진로교육의 목표, 내용, 측정이 동일선상에서 정 렬되어야 학생들의 실질적 발달과업에 초점을 맞춘 검사를 제대로 수행할 수 있다. 따 라서 이 연구의 목적은 산업발달에 따른 최신 직업용어 및 진로교육 목표를 요인설정 에 반영한 초등학생 진로발달 검사도구를 개발하는 데에 있다. 진로교육 프로그램의 효과성 측정에 실질적 도움을 주고자 현장교사들의 피드백을 반영하여 본 검사도구를 설계·제작하였다. 설문대상은 경기도 Y시에 소재하고 있는 8개의 초등학교 고학년 군 이며, 총 231개의 수집한 설문자료를 최종 분석에 활용하였다. 타당성 검토를 위하여 예비조사를 실시한 후, 적재요인이 부족하거나 설명량이 부족한 문항들을 수정·제거하 였다. 2차 전문가들의 의견을 반영하여 3개의 구인(자기이해, 진로탐색, 진로계획), 18 개의 최종 검사문항을 개발하였다. 이 검사도구의 신뢰도 및 타당도를 탐색적·확인적 요인분석을 통해 검증한 결과 각 하위요인은 .81이상의 신뢰도를 확인시켜주었으며 수 렴타당도, 판별타당도, 동시타당도 결과값은 수용할만한 수준을 보여주었다. 모쪼록 이 검사도구가 초등학교 진로교육 현장에서 실용적이면서도 간편하게 사용되기를 기 대하는 바이다.

Topic: Education and Learning Interventions
Subfield: Information Systems
Field: Computer Science
Domain: Physical Sciences
Excavation and Discovery

Habachi recounted their experience during the excavation and discovery of antiquities. He was very excited because he was armed with all he needed: a site, the money, and the diggers. He was anticipating the idea of finding wonderful things as they dug. The Fayoumis had traveled to Aswan on the night train. Few of the men had ever been so far south and Habachi started the journey with them in the third-class compartment. The fact that Habachi did indeed expect to find wonderful monuments was lost on the men. However, not all the monuments that they expected to find were found. But still, Habachi and his men were hopeful. Later on, his men unearthed the pavement of a chapel containing a portable shrine. Excavation continued, and each day, the diggers were finding several new objects.

Topic: Archaeology and Historical Studies
Subfield: Archeology
Field: Arts and Humanities
Domain: Social Sciences
Evaluation of Fracture Toughness on 800 MPa Grade HSLA Welds in Construction Machinery

The fracture toughness was measured at series of test temperature for an 800MPa grade HSLA steel and welds. Effect of welding processing on welds fracture toughness was discussed. The evident effect of rolling direction on fracture toughness is found. The fracture toughness for LT direction is much higher than that for TL direction. Base metal is in the catalogue of upper shelf ductile fracture above -60℃, ductile-brittle fracture mode transition occurs in the crack propagation at -80℃ . Fracture toughness of both shielded metal arc welding and gas metal arc welding is almost the same. Even through the fracture toughness of weld metals is higher than that of base metal at ambient temperature, the fracture toughness of weld metals is rapid decreased with decreasing test temperature. Fracture mode transition occurs at -20℃. This must be given attention in the service period of welded structures. The results will be valuable for the fracture evaluation of large scale construction machinery.

Topic: Fatigue and fracture mechanics
Subfield: Mechanics of Materials
Field: Engineering
Domain: Physical Sciences
Nitrogen Balance in the Post-Operative Patient Receiving Parenteral Nutrition

Nitrogen balance was measured in patients receiving balanced parenteral nutrition, most of whom were recovering from major surgery involving the alimentary tract. Their pre-operative nutritional status was found to be an important factor in relation to the nitrogen balance that was induced. Positive nitrogen balance did not occur in patients with little or no pre-operative weight loss, but when the weight loss was more than 6 kg. positive or very small negative nitrogen balances were readily induced. The urine creatine/creatinine ratio which is an indirect index of muscle metabolic activity can be used to anticipate the nitrogen balance that may be achieved in a post-operative patient receiving parenteral nutrition.

Topic: Clinical Nutrition and Gastroenterology
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Relationship Between Family Support and Adherence to Antiretroviral Medication in Patients with HIV at Bhayangkara Hospital, Indramayu, Indonesia

Introduction: Adherence to antiretroviral (ARV) treatment is one of the most critical factors in the successful management of HIV patients. Family support significantly influences treatment adherence, as family members are closest to the patient and are expected to provide motivation and monitor adherence to ARVs. This study aimed to determine the relationship between family support and ARV adherence among HIV patients. Methods: This study employed an analytic design with a cross-sectional approach. The population comprised HIV patients undergoing treatment in the Treatment Support Care Room (PDP) at Bhayangkara Indramayu Hospital, with an average monthly visit rate of 375 patients. A sample of 190 respondents was selected using the Z formula. The sampling technique employed purposive sampling based on inclusion and exclusion criteria. Bivariate analysis was performed using the chi-square test. Results: The findings revealed that 97 respondents (51.1%) reported good family support, and 146 respondents (76.8%) were compliant with taking ARV medication. The statistical analysis showed a p-value of 0.006 (α &lt; 0.05). Conclusion: There is a relationship between family support and adherence to ARV medication in HIV patients. It is recommended for nurses to educate families to provide support to HIV patients in ARV treatment.

Topic: HIV/AIDS Research and Interventions
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Do Patients Drink Enough Water? Actual Pure Water Intake Compared to the Theoretical Daily Rules of Drinking Eight 8-Ounce Glasses and Drinking Half Your Body Weight in Ounces

Water is vital for virtually every bodily process, but many people don’t drink enough water. We assessed how much actual water, on average, was drank by 100 consecutive patients from a well-ness clinic. The average water intake was about five 8-ounce glasses of water a day. When compared to the “drink eight glasses of water a day” rule, our sample was 3 glasses short. When compared to the “drink half your body weight in ounces” rule, our sample was 6 glasses short. Chronic, unintentional dehydration is so common that it may be better to consider many “dehydration diseases” such as asthma and allergies as well as non-infectious conditions and chronic pains to be identified as “indicators of body thirst” and not the conditions that today are considered “diseases of unknown etiology”. Physiologically there are parameters of dehydration that can be measured prior to one feeling “thirsty”, and therefore, simply drinking “ad libitum” or by natural instinct may not be adequate. Patients need to be told to drink more water and to keep a mental daily tally to be sure to optimize their hydration status to better their health.

Topic: Thermoregulation and physiological responses
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Stress‐Induced Dark Line Defect Formation in GaAlAs : Si LED's

Homojunction, graded bandgap LED's have been demonstrated to be highly reliable. During accelerated aging (30 mA forward bias, 250°C), these LED's generally degrade slowly without the formation of dark lines or dark spots. It has recently been found, however, that some LED's show a rapid reduction of light output even when aged without current bias at 200°C. <110> oriented dark lines appeared in the electroluminescence image of heavily degraded devices. To determine the source of the DLD's, the material quality of degraded LED's and unaged LED's from the same wafer was assessed using etch pitting and transmission cathodoluminescence. Examination of the devices revealed the presence of small pyramids, composed mainly of silicon, on the p‐surface of some of the LED's; no unusual features were found on the remaining LED's. A dislocation network surrounding each pyramid was found to be initially present. Due to bonding and thermal stresses applied to the pyramids, these networks enlarged with aging along the {111} planes and appeared as <110> DLD's in the electroluminescence image upon reaching the p‐n junction. In degraded LED's from pyramid‐free regions of the wafer with pyramids, <110> DLD's initiate at dicing damage and regions of the p‐surface subject to high bonding stress, i.e. edges and corners. The growth of these DLD's is identical to those initiating at pyramids. A similar study of LED's from a wafer that is totally free of pyramids shows that DLD's are not formed in devices that degrade slowly.

Topic: Semiconductor materials and devices
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
A Kind, Humane and Perfectly Honest Man

From A Brief Memoir of the Late Mr Thackeray (Edinburgh, 1864) pp. 17–31. This Memoir, a pamphlet reprinting Hannay's obituary of Thackeray in the Edinburgh Courant, of which he was Editor, was much admired and quoted at the time; Anthony Trollope for instance called it 'the only good attempt of its kind that I have seen'. It was further reprinted in Hannay's Characters and Criticisms (Edinburgh, 1865). Hannay (1827–73), a Scotsman, became a reporter, journalist and miscellaneous writer in 1845, after some unhappy years in the Navy. In 1848 he met Thackeray, whose works he had ardently admired and praised, and he greatly valued his friendship with the novelist, dedicating to him his own novel King Dobbs (1849). In 1852 he was engaged to annotate The English Humourists and see the book through the press; for Thackeray's cordial letter inviting him to do this (not in LPP) see George J. Worth, James Hannay (Lawrence, Kan., 1964) p. 96. Thackeray's patronage was valuable in other ways — he introduced him to the Quarterly Review, for instance — and Hannay reviewed Thackeray's novels in sundry periodicals warmly and intelligently though not uncritically. 'Quite better than the ordinary critics', said Thackeray of one notice (LPP, iii, 469). His Studies on Thackeray (1869) is a valuable pioneer assessment. Carlyle was the other contemporary whom he greatly admired. He was a staunch Conservative.KeywordsQuarterly ReviewGood AttemptOrdinary CriticRhetorical PowerTolerant LiberalityThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Topic: Irish and British Studies
Subfield: Sociology and Political Science
Field: Social Sciences
Domain: Social Sciences
The Production and Differentiation of Secondary Xylem in Xanthium pensylvanicum

The removal of leaves and buds from the shoot of Xanthium seedlings caused the cessation of xylem fiber differentiation in all internodes while allowing the production of cambial derivatives to continue toward both the xylem and the phloem. The potential xylem fibers developed into parenchymatous cells with thin cell walls. The vessels developed normally except for their small size. Cambial derivatives and vessels were produced linearly with time in intact plants (6.1 cells per file/day and 9.7 vessels per vascular bundle/day) and in decapitated plants (2.2 cells per file/day and 5.5 vessels per vascular bundle/day). Fiber production was linear with time in intact plants (163 fibers per vascular bundle/day) and did not occur in decapitated plants. When a single leaf was allowed to develop from a lateral bud of a decapitated plant, xylem fiber differentiation was restored for a period of time corresponding to the period of rapid expansion of the leaf blade. When the leaf passed the phase of rapid expansion, it no longer had an inductive effect on xylem fiber differentiation.

Topic: Allelopathy and phytotoxic interactions
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Interactive Calibration and Visual Programming of Reconfigurable Robotic Workcells

Small and Medium Enterprises can utilize robotic assembly solutions to improve their productivity. This is especially true if the assembly cells can be reconfigured to accommodate smaller batch sizes and flexible tasks. The Horizon 2020 project "ReconCell" aims to develop such a reconfigurable work cell. We created a Digital Twin of this reconfigurable robot cell in order to follow the Industry 4.0 approach. Users can visually model and develop different assembly processes for the digital reconfigurable robot cell, as well as execute them in simulation. To be able to transfer the simulated processes to hardware, the Digital Twin has to be consistent with the setup of the real robot cell: It needs to be thoroughly calibrated before it can be used. In this contribution, we present methods to conduct such a calibration interactively, and use the resulting consistency to conduct virtual commissioning of simulated assembly processes and transfer such processes to a physical robot cell.

Topic: Manufacturing Process and Optimization
Subfield: Industrial and Manufacturing Engineering
Field: Engineering
Domain: Physical Sciences