jimnoneill/paper-to-field-training huggingface dataset:
Human Atrial Natriuretic Peptide Gene Delivery Reduces Blood Pressure in Hypertensive Rats

Abstract Chronic infusion of atrial natriuretic peptide (ANP) has been shown to cause natriuresis, diuresis, and hypotension in rats and humans. We explored the effect of a continuous supply of ANP by somatic ANP delivery on genetically hypertensive rats. A DNA construct containing the human ANP gene fused to the Rous sarcoma virus 3′-long terminal repeat (RSV-LTR) was injected intravenously into spontaneously hypertensive rats (SHR) through the tail vein. Expression of human ANP in SHR was identified in the heart, lung, and kidney by radioimmunoassay and reverse transcription–polymerase chain reaction followed by Southern blot analysis. A single injection of naked ANP plasmid DNA (12.3 kb) caused a significant reduction of systemic blood pressure in young SHR (4 weeks old), and the effect continued for 7 weeks. The differences were significant at 1 to 2 weeks (n=6, P &lt;.05) and 3 to 6 weeks after injection (n=6, P &lt;.01) A maximal blood pressure reduction of 21 mm Hg in young SHR was observed 5 weeks after injection with ANP DNA (159.4±3.02 mm Hg, mean±SEM, n=6) compared with SHR injected with vector DNA alone (180.2±3.02 mm Hg, mean±SEM; n=6; P &lt;.01). Somatic gene delivery of human ANP DNA had no effect on the blood pressure of adult SHR (12 weeks old). After ANP gene delivery, there were significant increases in urinary volume and urinary potassium output (n=6, P &lt;.05) but not in body weight, heart rate, water intake, urinary sodium output, urinary creatinine, and urinary protein. Antibodies to human ANP or plasmid ANP DNA were not detected in rat sera. These results indicate that somatic delivery of the human ANP gene induces a sustained reduction of systemic blood pressure in young hypertensive rats and raise the feasibility of using ANP gene therapy for the treatment of human hypertension.

Topic: Membrane-based Ion Separation Techniques
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Prostitution Control in America: Rethinking Public Policy

Examination of policies regarding prostitution in the United States, including criminalization, decriminalization, and an alternative recommended by the author, a involving continued enforcement against street prostitution (coupled with greater municipal support services to help street prostitutes exit) and de facto decriminalization of indoor prostitution. It is argued that the two-track policy has advantages over blanket criminalization of all types of prostitution in that it involves more efficient use of the criminal justice system, is consistent with public preferences, and satisfies the harm-reduction principle.

Topic: Sex work and related issues
Subfield: Sociology and Political Science
Field: Social Sciences
Domain: Social Sciences
Results of Reconstruction of Orbital Wall Fracture With Bioresorbable Plate

Purpose: To investigate the long-term results and complications of orbital wall fracture reconstruction using the bioabsorbable orbital implant, Macropore orbital floor liner. Methods: This retrospective study included patients who underwent the reconstruction of an orbital wall fracture using Macropore orbital floor liner and completed a postoperative follow-up longer than 6 months. The enophthalmic values as well as the data of ocular movement and diplopia was collected from the medical records of each patient and analyzed. Results: A total of 35 patients were evaluated with an average follow-up period of 14.0 months. The average enophthalmic value of 29 patients, whose reconstruction was primarily indicated from enophthalmic tissue, was 0.90 mm at the last follow-up. No significant progression of enophthalmos was observed at postoperative 12, 18 and 24 months when compared with the enophthalmic value at postoperative 6 months. All 15 patients who have had the limitation of ocular movement or diplopia preoperatively resolved completely or improved to the degree that no clinically significant limitation or diplopia further existed. No complications such as dislocation of implant, infection, and aggravation of ocular limitation were observed during the follow-up period. Conclusions: Macropore orbital floor liner may be regarded as a useful implant in reconstruction of orbital wall fracture with no significant implant-related complications during its absorption.

Topic: Facial Trauma and Fracture Management
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Integrated approach for the comprehensive characterization of lipoproteins from human plasma using FPLC and nano-HPLC-tandem mass spectrometry

The implication of the various lipoprotein classes in the development of atherosclerotic cardiovascular disease has served to focus a great deal of attention on these particles over the past half-century. Using knowledge gained by the sequencing of the human genome, recent research efforts have been directed toward the elucidation of the proteomes of several lipoprotein subclasses. One of the challenges of such proteomic experimentation is the ability to initially isolate plasma lipoproteins subsequent to their analysis by mass spectrometry. Although several methods for the isolation of plasma lipoproteins are available, the most commonly utilized techniques require large sample volumes and may cause destruction and dissociation of lipoprotein particle-associated proteins. Fast protein liquid chromatography (FPLC) is a nondenaturing technique that has been validated for the isolation of plasma lipoproteins from relatively small sample volumes. In this study, we present the use of FPLC in conjunction with nano-HPLC-ESI-tandem mass spectrometry as a new integrated methodology suitable for the proteomic analysis of human lipoprotein fractions. Results from our analysis show that only 200 μl of human plasma suffices for the isolation of whole high density lipoprotein (HDL) and the identification of the majority of all known HDL-associated proteins using mass spectrometry of the resulting fractions.

Topic: Cancer, Lipids, and Metabolism
Subfield: Cancer Research
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
P1‐261: Identification of An Exonic Splicing Enhancer Variation in Exon 5 of the Alzheimer Disease Associated PICALM Gene

Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by memory and cognitive impairment and is the leading cause of dementia in the elderly. A growing number of genome wide association studies and subsequent replication studies have been recently published on late onset AD (LOAD), these studies identified several new susceptibility genes including phosphatidylinositol-binding clathrin assembly protein (PICALM) on chromosome 11. The aim of this study was to examine the entire coding sequence of PICALM to determine if the association could be explained by any previously undetected sequence variation. Such variation might explain, in whole or in part, the observed association. Therefore, we sequenced 48 cases and 48 controls homozygote for the risk allele for rs3851179 and of mixed APOE status. In this dataset, we did not find any new polymorphisms. However, rs592297 a known coding SNP that is part of an exonic splice enhancer region in exon 5 is in strong linkage disequilibrium with rs3851179. This SNP has altered heterozygosity and likely requires further study.

Topic: RNA Research and Splicing
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Modes of Address in Pictorial Art: An Eye Movement Study of Manet's <i>Bar at the Folies-Bergère</i>

Art-historical accounts of the last 200 years identify developments in the types, or “modes,” of address that a picture can present to a viewer as critical to the experience and evaluation of paintings. The authors focus on “anti-theatrical” theories of pictorial address and the complex and innovative “double relation” of absorption and acknowledgment introduced by the painter Edouard Manet. They report a case study of Manet's A Bar at the Folies-Bergère investigating expert and novice spectators' eye movements and utterances in response to the painting to find evidence that viewers seek resolution of the complex “double relation” that the theories describe.

Topic: Multisensory perception and integration
Subfield: Experimental and Cognitive Psychology
Field: Psychology
Domain: Social Sciences
Hereditary Thrombotic Thrombocytopenic Purpura: Microangiopathic Hemolytic Anemia, Thrombocytopenia, and Renal Insufficiency Occurring in Consecutive Generations

Microangiopathic hemolytic anemia, thrombocytopenia, and renal insufficiency occurred in a mother an daughter when each was in her third decade. The mother had prominent neurological findings, fever, and expired from renal insufficiency. Her daughter's only major symptom was renal insufficiency. The similarity of the mother's illness to thrombotic thrombocytopenic purpura and of the daughter's to adult hemolytic uremic syndrome suggests that these diseases are variants of a single clinical disorder, while their occurrence in direct descendents strengthens the postulate that in selected instances a genetic predisposition to these disorders may be important.

Topic: Complement system in diseases
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Differentiating and Synthesizing Socially Responsible Consumption: A Multi-Level Conceptual Framework and Future Research Directions

Important societal and environmental issues need to be addressed in the current day. The study of socially responsible consumption (SRC) has emerged as a discipline that prepares academics and professionals to act as communities capable of addressing such issues. We aim to extend the current knowledge of SRC in the business and management domains by first differentiating the concept from other interchangeably used concepts such as ‘ethical consumption,” ‘green consumption,” ‘socially conscious consumption,’ and ‘sustainable consumption,’ followed by a systematic literature review approach. We show that SRC is conceptually distinct and encompasses the broadest scope compared to the four similar concepts within the business and management domains. Furthermore, a systematic review of 64 articles published over the past decade (2013–2023) has revealed various themes related to consumers, consumption, firms, and products. We discuss these themes from both macro and micro perspectives and develop a multi-level conceptual framework to advance the holistic understanding of SRC in business and management domains. Additionally, we outline future research avenues for each theme at both the macro and micro levels as well as in terms of theory, context, and methods. Ultimately, this study enriches the knowledge of SRC in the business and management domains by (A) differentiating the construct and defining its conceptual boundaries, (B) synthesizing SRC research and identifying emerging research themes at both macro and micro levels, and (C) offering a multi-level conceptual framework and potential future research directions (FRDs).

Topic: Environmental Sustainability in Business
Subfield: Marketing
Field: Business, Management and Accounting
Domain: Social Sciences
Decolonization of Academia: Is the Word Latinx a Form of Colonization?

Abstract There has been debate for some years around the term Latinx and how members of the community identify with it. Originally, the term emerged in academia as a gender-neutral option to counter the term Latino/a or Latin@ and to try to establish a differentiation from the general term of Hispanic that many consider to not be representative of the community. However, the inclusion and adoption of the term has generated some debate. For example, members of the community have different views on if the term is applicable in a Spanish speaking context, in which cases some might advocate for the term Latiné as being the linguistically gender-neutral word created by those in Spanish speaking countries. Several studies have been conducted around the use and understanding of the term in different contexts, however, there has not been an extensive exploration of the topic in engineering. The purpose of this work is to explore how engineering students -who identify as Latinx/a/o/é at a large research university engage with the term Latinx. We conducted a survey to ask students about their understanding and adoption of the term Latinx, their preferred term, and their familiarity with different terms such as Latin@, Latin*, Latinu, Latini, Hispano, etc. The survey was administered online and data were analyzed using descriptive statistics to map students' preferences and a thematic analysis approach to identify emerging themes in the open-ended questions (e.g., why do you identify (or not) with the term Latinx?) Our results provide a better understanding of engineering students' perceptions around various identifying terms and we engage critically in a discussion of the importance of understanding how members of the community perceive themselves. We provide implications for research, practice, and administration.

Topic: Higher Education Research Studies
Subfield: Education
Field: Social Sciences
Domain: Social Sciences
Teyl by Using Songs and Games in Students’ Learning Motivation

This study aimed at finding out whether the use of songs and games in teaching English to young learners give an effect in students' learning motivation at MI Brawijaya Trowulan, Mojokerto. In order to achieve the goal of the study, quasi experimental design was utilized. In this study was used 2 classes as the sample, they are experimental group and control group. The questionnaires were used to collect the data. Then the data were analyzed by using Analysis of Covariance (ANCOVA) through SPSS 24. In the process of English teaching learning, the researcher applied 2 songs which are “Jobs Song” and “Eat and Drink All Day Long” and 2 games which are “Suit Game” and “The Mime Game”. The study revealed that TEYL by using songs and games could make young learners enjoy and feel fun the learning process, enthusiastic, become confident to perform, and involved to be participant. Consequently, the result of this study has enhanced the students’ learning motivation.

Topic: Educational Methods and Outcomes
Subfield: Education
Field: Social Sciences
Domain: Social Sciences
CCDC 1855964: Experimental Crystal Structure Determination

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Topic: Crystallization and Solubility Studies
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Towards sub-nyquist cognitive radar

&lt;p&gt;Cognitive radar (CR) has recently been considered as a natural next step for traditional radar. The cognitive property assumes both transmitter and receiver to be able to dynamically adapt to environment changes. In this work, we propose to exploit sub-Nyquist sampling methods that have been originally proposed to reduce the sampling rate bottleneck at the receiver. For CR, we extend this approach by only transmitting the spectral bands that are to be sampled and processed on the receiver side. By considering transmitted and received pulses with dynamic support composed of several narrow bands, we pave the way to sub-Nyquist CR. Both software and hardware simulations demonstrate dynamic transmitted signal support, still allowing for delayDoppler recovery.&lt;/p&gt;

Topic: Radar Systems and Signal Processing
Subfield: Aerospace Engineering
Field: Engineering
Domain: Physical Sciences
Preliminary Experimental Evaluation of a Miniaturized Hall Thruster

A miniaturized 50 W Hall thruster has been designed and manufactured at the Massachusetts Institude of Technology based on the apriori developed scaling model. The thruster has been experimentally tested for operation and the collected data were compared to those for existing larger Argon-operated devices. Qualitative comparison showed good match in performance as well as similarity in general trends. Detailed thrust measurements will be performed to quantitatively assess the microthruster performance to verify the scaling model.

Topic: Plasma Diagnostics and Applications
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Sequential reduced‐intensity chemotherapy for allogeneic hematopoietic stem cell transplantation in acute myeloid leukemia with rare cytogenetic abnormalities transformed from Fanconi anemia

Table S1. Studies of FA patients with advanced myeloid malignancy who did or did not receive chemotherapy prior to HSCT Table S2. Cases of pediatric acute myeloid leukemia with RUNX1-CBFA2T3 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

Topic: Acute Myeloid Leukemia Research
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
A HYPOTHESIS BEARING ON DISEASE THERAPY

To the Editor: —I desire to advance a hypothesis as a serviceable conception of disease processes. Certain organisms or the toxins elaborated by them or poisons otherwise produced initiate specific processes in certain structures. These processes, if unchecked, run a definite course, and tend to produce characteristic symptoms. In many individuals so afflicted there comes a time in which the injured structures are sensitized not only to the specific proteins elaborated by the causal organisms, but also to allied proteins elaborated by other organisms, and perhaps to allied bodies chemically produced. In such instances the structural change already initiated tends to run as it would if the controlled original cause were still acting. Thus a tabetic, as a result of vigorous antisyphilitic treatment, may become symptomless, and his cerebrospinal fluid may be brought to normal; but if he should suffer from an infection, as acute appendicitis, the patient's chief and

Topic: Syphilis Diagnosis and Treatment
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Online color camera calibration

This paper presents a practical approach to online color camera calibration under changing illumination conditions. Calibration is based on a color camera model and color constancy. In the first stage of the calibration a reconstruction of the current illuminant is performed based on an image taken from a scene with a calibrated color set. Using the updated power spectrum distribution of the current illumination the output camera values are corrected. Based on the corrected camera output values reconstruction of the reflectance spectrum of a random color object is performed and color features of the object can be determined under a required illuminant color temperature. The simulation experiments of the algorithm and results of tests on real images are discussed and the performance of the calibration is evaluated. The approach provides a real time color camera calibration procedure for many applications of machine vision.

Topic: Color Science and Applications
Subfield: Atomic and Molecular Physics, and Optics
Field: Physics and Astronomy
Domain: Physical Sciences
Right Field, Left Field: some creative approaches to teaching script

In this article I review what I perceive to be two distinct historic approaches to teaching script-writing. The first, reflective and distanced in quality, treating the writing process as something which can be guided and developed by discourse and argument; the second, experiential and writer-centred, seeking to support the intuitive, less tangible side of the creative process. Employing case studies taken from my own teaching experience, I come to the conclusion that it is important, especially given current trends and pressures in film education, to find ways of nurturing the experiential side. However I show that it is possible, in the best writing workshops to create situations where both approaches can contribute and creatively intertwine, reflecting in important ways the nature of the film-writing process itself.

Topic: Artistic and Creative Research
Subfield: Visual Arts and Performing Arts
Field: Arts and Humanities
Domain: Social Sciences
Variability analysis for noisy physiological signals: A simulation study

Physiological monitoring is prone to artifacts originating from various sources such as motion, device malfunction, and interference. The artifact occurrence not only elevates false alarm rates in clinics but also complicates data analysis in research. When techniques to characterize signal dynamics and the underlying physiology are applied (e.g., heart rate variability), noise and artifacts can produce misleading results that describe the signal artifacts more than the physiology. Signal quality metrics can be applied to identify signal segments with noise and artifacts that would otherwise lead analyses to produce non-physiologic or misleading results. In this study we utilized simulated electrocardiogram signals and artifacts to demonstrate effects of noise on heart rate variability frequency domain methods. We then used these simulations to assess an automated artifact correction algorithm that included a signal quality index comparing electrocardiogram beats to a beat template. Simulation results show that the proposed algorithm can significantly improve estimation of signal spectra in presence of various artifacts. This algorithm can be applied to automatically clean real world physiological time series before conducting variability analysis.

Topic: Healthcare Technology and Patient Monitoring
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
<i>Drosophila melanogaster</i>Activating Transcription Factor 4 Regulates Glycolysis During Endoplasmic Reticulum Stress

Abstract Endoplasmic reticulum (ER) stress results from an imbalance between the load of proteins entering the secretory pathway and the ability of the ER to fold and process them. The response to ER stress is mediated by a collection of signaling pathways termed the unfolded protein response, which plays important roles in development and disease. Here we show that in Drosophila melanogaster S2 cells, ER stress induces a coordinated change in the expression of genes involved in carbon metabolism. Genes encoding enzymes that carry out glycolysis were up-regulated, whereas genes encoding proteins in the tricarboxylic acid cycle and respiratory chain complexes were down-regulated. The unfolded protein response transcription factor Atf4 was necessary for the up-regulation of glycolytic enzymes and Lactate dehydrogenase (Ldh). Furthermore, Atf4 binding motifs in promoters for these genes could partially account for their regulation during ER stress. Finally, flies up-regulated Ldh and produced more lactate when subjected to ER stress. Together, these results suggest that Atf4 mediates a shift from a metabolism based on oxidative phosphorylation to one more heavily reliant on glycolysis, reminiscent of aerobic glycolysis or the Warburg effect observed in cancer and other proliferative cells.

Topic: Endoplasmic Reticulum Stress and Disease
Subfield: Cell Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences