jimnoneill/paper-to-field-training huggingface dataset:
Dilated aorta in CNOT3-related neurodevelopmental disorder: ‘expanding’ the phenotype

Introduction Neurodevelopmental disorders (NDDs) comprise conditions that emerge during the child’s development and contribute significantly to global health and economic burdens. De novo variants in CNOT3 have been linked to NDDs and understanding the genotype–phenotype relationship between CNOT3 and NDDs will aid in improving diagnosis and management. Methods In this study, we report a case of a patient with CNOT3 -related NDD who presented with progressive aortic dilatation, a feature not reported previously. Results Our patient presented with intellectual disorder, dysmorphic facial features, and cardiac anomalies, notably progressive aortic dilatation – a novel finding in CNOT3 -related NDD. Genetic testing identified a de novo 6.3 kbp intragenic deletion in CNOT3 , providing a possible genetic basis for her condition. Conclusion This study presents the first case of CNOT3 -related NDD in Southeast Asia, expanding the phenotype to include progressive aortic dilatation and suggesting merit in cardiac surveillance of patients with CNOT3 -related NDD. It also emphasizes the importance of genetic testing in diagnosing complex NDD cases as well as reanalysis of ‘negative’ cases using advanced sequencing technologies to uncover potential hidden genetic etiologies in undiagnosed NDDs.

Topic: Congenital heart defects research
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Smart Agriculture Farming with Image Capturing Module

Agriculture is an important factor for the wellbeing of a country. Though India is not the largest agriculture nation in the world, it contributes a considerable amount to the world's agriculture production. But due to various factors, the growth rate is dropping in recent times. This is one of the main reasons for the need of development in the field of agriculture. With the recent technological improvements, the field of agriculture can be automated with the help of Internet of Things (IoT), Wireless Sensor Networks (WSN), Cloud Computing & Big Data Analysis. The current agriculture network systems do not match with the speed and thus the storage capacity of various other factors also come into play. For this to improve, the researchers suggest for Agriculture as a Service (AaaS) model which is basically an Application as a Service. IoT helps to share the data which can be processed using Big Data techniques by storing into a cloud. This is the modern approach to the Agricultural Networking System problem which will improve the Quality of Service (QoS). This paper focuses on building such a system with the camera module. This module helps to capture the images which can be used to analyze the type of leaf and if it is affected by any disease. The information regarding the same can be sent to the users' mobile using the GSM technology.

Topic: Smart Agriculture and AI
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Lesbians, Feminism, and Psychoanalysis

Get a feminist perspective on important changes in psychoanalysis! Lesbians, Feminism, and Psychoanalysis: The Second Wave examines recent changes in psychoanalysis that have opened the door for new perspectives on same-sex desire. Authors from a variety of disciplines and theoretical orientations combine feminism with psychoanalytic and postmodern theories to celebrate diversity in gender and sexual experience. This collection of lesbian-affirmative writings addresses transference and countertransference, gender subjectivities, privilege and racism, therapist homophobia, and violence in lesbian relationships. In the past decade, psychoanalysis has undergone changes in clinical theory that have led to views on human sexuality that are less focused on what is "normal" and therapy practices that resist attempts to fit individuals into prescribed developmental models. Lesbians, Feminism, and Psychoanalysis presents a variety of backgrounds (psychiatry, psychology, and social work), analytic training (formal institute training, study groups, supervision), and theoretical perspectives (self-psychology, object relations, relational psychoanalysis, feminist theory, queer theory, postmodernism, Lacanian theory) unified by the healing power of psychoanalytically informed theory and practice. The book is divided into three sections—"Community: Personal and Political," "Ongoing Clinical Issues," and "New Thinking on Sexuality and Gender," addressing lesbian tomboy development, the queering of relational psychoanalysis, how attachment theory and intersubjectivity can contribute to newer gender theory, and including: interviews with lesbian psychoanalytic foremothers Joanne Spina, Lee Crespi, and Judy Levitz Dr. Darla Bjork's account of her journey to becoming an openly lesbian therapist contrasting views on transference and countertransference from gay and lesbian therapists and much more! Lesbians, Feminism, and Psychoanalysis: The Second Wave is an essential practical resource for clinicians and a vital classroom tool for academics working in psychology, social work, psychoanalysis, gender and women's studies, queer studies, and lesbian and gay studies.

Topic: Psychotherapy Techniques and Applications
Subfield: Clinical Psychology
Field: Psychology
Domain: Social Sciences
CCDC 761953: Experimental Crystal Structure Determination

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Topic: Crystallization and Solubility Studies
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Anticancer effects of piperine-free Piper nigrum extract on cholangiocarcinoma cell lines

Pharmacognosy Magazine,2020,16,68,28-38.DOI:10.4103/pm.pm_288_19Published:March 2020Type:Original ArticleAuthors:Aman Tedasen, Araya Khoka, Siribhorn Madla, Somchai Sriwiriyajan, and Potchanapond Graidist Author(s) affiliations:Aman Tedasen1, Araya Khoka2, Siribhorn Madla3, Somchai Sriwiriyajan2, Potchanapond Graidist1 1 Department of Biomedical Sciences, Faculty of Medicine; The Excellent Research Laboratory of Cancer Molecular Biology, Prince of Songkla University, Hat Yai, Songkhla, Thailand 2 Department of Pharmacology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand 3 Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand Abstract:Background: Black pepper (Piper nigrum L.) is widely used as a traditional medicine, including usage for pain relief, fevers, as well as an anticancer agent. Previously, we reported that piperine-free P. nigrum extract (PFPE) inhibited breast cancer in vitro and in vivo. Objective: In this present study, we explored the anticancer effects of PFPE on cholangiocarcinoma (CCA). Materials and Methods: 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to analyze cytotoxic potential of PFPE whereas deoxyribonucleic acid (DNA) fragmentation followed by Western blot analysis were used. Results: PFPE composed of alkaloid, flavonoid, amide, lignans, opioid, and steroid. This crude extract represented cytotoxic effect against CCA cells which stronger than dichloromethane P. nigrum crude extract and piperine, especially on KKU-M213 (median inhibition concentration [IC50] at 13.70 μg/ml) and TFK-1 (IC50at 15.30 μg/ml). Interestingly, PFPE showed lower cytotoxicity against normal human cholangiocyte MMNK-1 cells (IC50at 19.65 μg/ml) than KKU-M213 and TFK-1 cells. Then, the molecular mechanisms of PFPE were firstly evaluated by DNA fragmentation followed by Western blot analysis. The degradation of DNA was observed on KKU-M213 and TFK-1 cells after treatment with PFPE at day 2. Then, proliferation proteins including topoisomerase II, AKT8 virus oncogene cellular homolog, avian myelocytomatosis virus oncogene cellular homolog, cyclin D1, signal transducer and activator of transcription 3, cyclooxygenase-2, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) were decreased and p21 was increased. Furthermore, apoptotic proteins, such as tumor protein p53, Bcl-2-associated X protein, and p53 upregulated modulator of apoptosis were upregulated. Meanwhile, antiapoptotic protein B-cell lymphoma 2 was down-regulated. Conclusion: These results indicated that PFPE inhibited CCA through the down-regulation of cell proliferation and induction of apoptosis pathway. Keywords:Anticancer, Apoptosis, cell proliferation, cholangiocarcinoma, Piper nigrumView:PDF (2.33 MB)

Topic: Piperaceae Chemical and Biological Studies
Subfield: Pharmacology
Field: Pharmacology, Toxicology and Pharmaceutics
Domain: Life Sciences
Effects of a Combination of Berberis aristata, Silybum marianum and Monacolin on Lipid Profile in Subjects at Low Cardiovascular Risk; A Double-Blind, Randomized, Placebo-Controlled Trial

The aim of this study was to evaluate the efficacy and safety of an anti-hypercholesterolemic agent containing Berberis aristata, Silybum marianum and monacolin K and KA in a sample of Caucasian patients at low cardiovascular risk according to Framingham score. The primary outcome was to evaluate the effects of this nutraceutical combination on lipid profile; the secondary outcome was to evaluate the effect on some inflammatory markers, in particular high sensitivity C-reactive protein and tumor necrosis factor-α interleukin-6. One hundred and forty-three patients were randomized to placebo or Berberol® K, once a day, during the dinner, for 3 months, in a randomized, double-blind, placebo-controlled trial. We recorded a significant reduction of fasting plasma glucose with Berberol® K compared to placebo (−12.2%, p &lt; 0.05). Moreover, we recorded an increase of fasting plasma insulin with Berberol® K both compared to baseline and to placebo (+9.9%, p &lt; 0.05). Accordingly, the homeostasis model assessment (HOMA) index obtained after treatment with Berberol® K was lower than the one in the placebo group (−2.8%, p &lt; 0.05). No variations of lipid profile were observed with placebo, while there was a significant decrease of total cholesterol (−20.5%, p &lt; 0.05), triglycerides (−17.7%, p &lt; 0.05), and low density lipoprotein (LDL) cholestero (−27.8%, p &lt; 0.05) with Berberol® K, compared to placebo. There was a decrease of high sensitivity C-reactive protein (−30.8%, p &lt; 0.05), and interleukin-6 (−25.0%, p &lt; 0.05), with Berberol® K compared to placebo. In conclusion, combining different hypocholesterolemic nutraceutical agents such as Berberis aristata, Silybum marianum and monacolin K and KA could be effective and safe to obtain a reduction of lipid profile and an improvement of inflammatory parameters.

Topic: Berberine and alkaloids research
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Improved Gecko Inspired Dry Adhesives Applied to the Packaging of MEMS

An alternative method of manipulating fragile MEMS devices during fabrication has been developed. A specialized manual or robotic end effector utilizing a gecko inspired adhesive can replace the slow and costly manual tweezers used by the MEMS foundry Micralyne Inc. Improving the adhesive fabrication techniques greatly increased adhesion strength, which was tested with a custom programmed apparatus. An antistatic, nanoparticle-polymer composite was developed for the adhesive material to prevent static discharge. The improved adhesive was then used with a specially designed end effector that contacts only the bonding pads or perimeter of the MEMS device, preventing damage to sensitive central structures. Using special techniques that only rely on shearing motions, the adhesive is engaged and disengaged to move the MEMS device between common carriers such as tacky Gel-Paks. This is the first part of a project to implement such adhesives into commercial manufacturing of MEMS or other sensitive electronic devices.

Topic: Nanofabrication and Lithography Techniques
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
ROTATIONAL ACETABULAR OSTEOTOMY FOR SYMPTOMATIC HIP DYSPLASIA IN PATIENTS YOUNGER THAN 21 YEARS

Adolescent and young adult patients with a history of DDH and residual hip dysplasia tend to have an early presentation of symptoms. In these patients, a severe acetabular dysplasia, aspheric femor...

Topic: Hip disorders and treatments
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
A FRAMEWORK TO EVALUATE UNIVERSITY STUDENTS' USE OF DERIVE SOFTWARE TO COMPREHEND AND APPLY THE CONCEPT OF DEFINITE INTEGRAL

This study documents the type of mathematical competence that first year university students develop as a result of working on series of problems that involve definite integral concepts. During the problem solving sessions students had the opportunity of using DERIVE software to solve the tasks, that were specially designed (Camacho, Depool and Santos, 2004), to comprehend and apply definite integral concepts. In this report, we sketch a framework to evaluate the students’ mathematical competences that emerge during the sessions and value the importance, for instructors, to think of the curriculum contents in terms of problems or activities that guide the students’ learning trajectories within CAS (Computer Algebra Systems) environment.

Topic: Mathematics Education and Teaching Techniques
Subfield: Education
Field: Social Sciences
Domain: Social Sciences
Radiation safety management for diagnostic radiation generators and employees in animal hospitals in Korea

A nationwide survey on radiation safety management in Korean animal hospitals was conducted.By 2013, 53 radiation generators were registered as veterinary medical devices (41 X-ray generators and 12 computed tomography scanners).Additionally there were six approved laboratories for radiation equipment and protection facility, and five approved laboratories for radiation exposure of employees, respectively.By March 2013, 2,030 out of 3,829 animal hospitals operated radiation-generating devices.Among these devices, 389 (19.2%) out of 2,030 were not labeled with the model name and 746 (36.7%) were not labeled with production dates.Thus, most veterinary X-ray generators were outdated (42.6%) and needed replacements.When periodic inspections of 2,018 animal hospitals were performed after revision of the Veterinarians Act in 2011, the hospitals were found to be equipped with appropriate radiation generators and protection facilities.Among 2,545 employees exposed to radiation at the hospitals, 93.9% were veterinarians, 4.3% were animal nurse technicians, and 18% held other positions.Among 169 employees supervised by administrators, none of those had a weekly maximum operating load that exceeded 10 mA•min.This study suggests that the radiation safety management system of animal hospitals was general good.

Topic: Radiation Dose and Imaging
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
CHARACTERIZATION OF THE AUXIN SYNTHESIS GENES OF ERWINIA HERBICOLA PV. GYPSOPHILAE

We present the DNA sequence of some 3590 base pairs from the virulence plasmid of the crown-gall-forming bacterium Erwinia herbicola pv. gypsophilae strain PD713. This region includes the entire transcription unit of an operon encompassing the IAA synthesis genes. These genes, designated iaaM and iaaH , reside on a 78-MDa native plasmid. The sequence analysis indicates that the operon contains two open reading frames of 562 ( iaaM ) and 460 amino acids ( iaaH ), corresponding to proteins with molecular weight of 62,473 and 49,300 daltons, respectively. When cloned and expressed in minicell-producing E. coli , this sequence encodes production of two proteins, 49 and 62 kDa. Both genes show moderate to high homology to IAA synthesis genes from other plant-associated bacteria. Analysis by primer extension of the transcription initiation site located it 63 bases upstream of the translation initiation codon.

Topic: Plant Pathogenic Bacteria Studies
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
In Response

Although we are aware that Staphylococcus enterocolitis (SEC) can occur after antibiotic use and that it is an easily overlooked cause of nosocomial enterocolitis and sepsis, we disagree with Drs. Naughton and Mannion's opinion that our case is more likely caused by SEC than due to foreign body–associated infection.1 Most cases associated with SEC present with large volume, “cholera-like” diarrhea associated with patchy necrotizing segmental gangrene and bowel wall thickening.2 The exploratory laparotomy findings included normal-appearing large colon with stool in the descending and sigmoid colon with no evidence of ischemic colitis, perforation, or diverticulosis, and the small bowel appeared normal except for an isolated 2-inch transmural focal area of ischemia in the jejunum associated with hemorrhagic rugose mucosa per surgical pathology report. Furthermore, SEC associated with antibiotic use is typically due to flouroquinolones, with the median duration of antimicrobial therapy being 7 days. The fact that this patient received 3 doses of cefazolin makes this scenario less likely.3 Clinically, our patient was in septic shock, had positive blood cultures for Staphylococcus aureus, positive cultures for staphylococcus from the neck abscess, and isolated small intestinal perforation associated with ischemia. The indigo scan showed no signs of infection at the surgical site. Although we would rather attribute this patient's postoperative complication to a source such as SEC, we reiterate our conclusion that a more likely cause of the bacteremia was infection at the interscalene catheter site.4 Steven R. Clendenen, MD Christopher B. Robards, MD Department of Anesthesiology Mayo Clinic Florida Jacksonville, Florida [email protected]

Topic: Clostridium difficile and Clostridium perfringens research
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Creating a Change Management Model for Business Process Implementation

Implementation of business processes, or any form of new working methods, is crucial for a company to adapt to its environment or follow its vision by realising the company strategy. Cargotec set out to unify its business processes in 2007. Unifying offering development processes was one crucial part of this. In order to succeed in the large scale global change the company wanted to study possible change management models to be used during the changes.&#13;\n&#13;\nThe research goes through process theories in order to clarify the nature of the change. Various change management models are presented and change as a phenomenon is studied from various perspectives to create a change management model that would be most suitable for Cargotec and its business process implementation. Furthermore, issues such as factors increasing the feeling of control in change, whether change can be planned and led as a process and what is the proper amount of stakeholder participation in planning and executing the changes are studied. As a conclusion a change management model reaching vertically through the organisation and incorporating flexibility is presented.&#13;\n&#13;\nThe changes are studied as a case study from the perspective of a participant observer. The findings are presented and analysed in order to see if the change management model would be applicable in similar changes in the future. The process implementation was not done according to the model in the studied organisation. That is, by managing the change systematically on every level of the organisation and establishing a sponsorship spine for efficient communication. The problems in the chosen approach were discussed with the process owner and the conclusion was that most of the problems would have been avoided by working according to the change management model. It remains to be seen how the model yields results in the upcoming change projects.&#13;\n&#13;\nThe study achieved what it was meant to. A model for managing change was created for Cargotec and through the study a powerful insight to problems emerging during process changes is gained.

Topic: Hermeneutics and Narrative Identity
Subfield: Philosophy
Field: Arts and Humanities
Domain: Social Sciences
Old Testament Stories: Their purpose and their art

Judging from repetitious appearances of her marital arms in the painted line-endings, the Psalter-Hours John Rylands Library Latin MS 117 probably belonged to Jeanne of Flanders (c.1272–1333), daughter of Count Robert III of Flanders and in 1288 second wife to Enguerrand IV of Coucy. Yet the line-endings also contain some 1,800 diminutive painted escutcheons, many of which refer to other members of the local nobility active during the 1280s. This study, based on an exhaustive survey of the total heraldic and codicological evidence, suggests that the majority of the extant Psalter predated the Hours and that the two parts were combined after the 1288 marriage. The ‘completed’ manuscript bears witness to major events that unfolded in and around the Coucy barony over the course of the decade. It suggests a complex relationship between Jeanne of Flanders and a lesser member of the local nobility, a certain Marien of Moÿ, who may have served as her attendant.

Topic: Medieval Literature and History
Subfield: Classics
Field: Arts and Humanities
Domain: Social Sciences
Photodynamic therapy laser system

Photodynamic therapy (PDT) treatment is a new treatment for tumour and Dermatology. With the successful development of the second-generation photosensitizer and the significant manifestations in clinics, PDT has shown a more extensive application potentials. To activate the photosensitizer, in this paper, we present a GaAs-based diode laser system with a wavelength of 635 nm. In this system, to prolong the working life-time of the diode lasers, we use specific feedback algorithm to control the current and the temperature of the diode laser with high precision. The clinic results show an excellent effect in the treatment of Condyloma combined with 5-ALA.

Topic: Photodynamic Therapy Research Studies
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Improving Learning Outcomes to Hit The Ball In Castle Games Through Play Methods in Class Iv Students of Waesama 17 Private Elementary School

The purpose of this research is to improve the learning outcomes of hitting the ball in a baseball game through the playing method for fourth graders of Waesama 17 Private Elementary School. The research method used in this research is to use classroom action research. The subjects of this study were 18 grade IV students of Waesama Private Elementary School 17. After processing and analyzing the data, the results of the first cycle were obtained based on the table of learning outcomes in the first cycle, there were 11 students who had completed 61.1%, while 7 students had not completed 38.8%. Cycle II based on the recapitulation table of student learning completeness in cycle II there were 18 students who completed or (100%) and there were no students who did not complete or (0%). From the results of the analysis and calculation of the data, it can be concluded that the playing method is effective for increasing the learning outcomes of hitting the ball in a baseball game for fourth graders of Waesama 17 Private Elementary School.

Topic: Sports and Physical Education Research
Subfield: Physical Therapy, Sports Therapy and Rehabilitation
Field: Health Professions
Domain: Health Sciences