jimnoneill/paper-to-field-training huggingface dataset:
(Digital Presentation) An Integrated Platform for Immune Profiling and Cytokine Monitoring

In the realm of cancer immunotherapy, the pivotal role of immune cells in driving efficacy, and the delicate balance maintained by cytokines between efficacy and potentially life-threatening side effects, cannot be overstated. Hence, precise in situ immune profiling and close scrutiny of critical cytokines are imperative for assessing the features associated with immunotherapeutic interventions. Unfortunately, existing in vitro assessment methods suffer from drawbacks such as being endpoint, time-intensive, and demanding large sample volumes. To address these limitations, we present an innovative solution: an integrated real-time 3D vascularized blood-brain barrier (BBB) chip. This cutting-edge platform faithfully replicates the BBB environment infiltrated by immune T cells while simultaneously enabling the monitoring of immune profiling. Crucially, it facilitates the real-time detection of cytokines during CAR T cell interactions with various cell types. This comprehensive monitoring encompasses crucial aspects such as T cell and monocyte migration, cancer cell recognition, immune cell activation, and cytokine and neurotransmitter dynamics. Furthermore, the chip allows for the evaluation of the dynamics of therapeutics aimed at managing side effects associated with immunotherapy. A key highlight of our approach is the ability to detect cytokines directly within the biological milieu. This method exhibits an impressive detection range spanning from 1 to 10,000 pg/mL, requiring only a minimal sample volume of 4 μL. The innovative sensing mechanism utilizes pillar microarrays, where anti-cytokine capture antibodies (CAbs) immobilized as the primary layer efficiently capture cytokines. Paired detection antibodies (DAbs) then facilitate signal detection, enabling simultaneous detection of multiple cytokines within small sample sizes through parallel processing. By providing a means to effectively monitor the dynamics of immune therapy-induced factor release without perturbing the cancer microenvironment, our integrated chip empowers researchers to delve deeper into the intricacies of immunotherapy while enhancing its safety profile. This advancement holds significant promise for improving the outcomes of cancer treatment, ushering in a new era of precision and efficacy in cancer immunotherapy.

Topic: Advanced Biosensing Techniques and Applications
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
First Field Trial of a Coiled Tubing for Exploration Drilling

ABSTRACT Within the scope of comprehensive efforts to reduce the cost of exploration drilling, Elf Aquitaine has performed with Dowell Schlumberger a first trial of open-hole coiled tubing drilling in the Paris Basin. The purpose of this trial was to determine the feasibility of using coiled tubing drilling units instead of conventional rotary rigs to drill slim hole exploration wells. The feasibility has been verified and applies to some specific prospects. A second trial is planned in 1992 in the same basin.

Topic: Drilling and Well Engineering
Subfield: Ocean Engineering
Field: Engineering
Domain: Physical Sciences
ProcessingWindow Queries in Wireless Sensor Networks

The existing query processing techniques for sensor networks rely on a network infrastructure for query propagation and data collection. However, such an infrastructure is very susceptible to network topology transients that widely exist in sensor networks. In this paper, we propose an infrastructure-free window query processing technique for sensor networks, called itinerary-based window query execution (IWQE), in which query propagation and data collection are combined into one single stage and executed along a well-designed itinerary inside a query window. We study the parameters for setting up an itinerary (e.g., width and route) and incorporate into IWQE three data collection schemes based on different performance trade-offs. Finally we demonstrate, by extensive simulations, the superior energy-time efficiency, robustness, and accuracy of IWQE over the current state-of-the-art techniques in supporting window queries under various network conditions.

Topic: Energy Efficient Wireless Sensor Networks
Subfield: Computer Networks and Communications
Field: Computer Science
Domain: Physical Sciences
0795 Implementation of the Pediatric Craniofacial Screening Tool for Sleep Disordered Breathing (PCSS): An Observational Pilot Study

Abstract Introduction Pediatric sleep-disordered breathing (SDB) is highly prevalent and associated with craniofacial risk factors not routinely assessed. We developed the Pediatric Craniofacial Screening Tool for Sleep (PCSS) to assess which craniofacial risk factors are closely correlated with SDB symptoms in children. Methods Pilot study of children ages 5-12y recruited from eight dental sites across the country to participate in the PCSS screening by dental providers. The primary outcome was SDB measured by the validated PSQ-SRBD questionnaire (22 items, range 0-1, and &amp;gt;0.33 signifying increased SDB risk). The predictors included PCSS total score and individual items comprising of 21 craniofacial features. Neighborhood socioeconomic status (NSES) was characterized by using the Area Deprivation Index (ADI). Correlation and regression analyses modeled the association between PSQ-SRBD and total PCSS score. To assess inter-rater reliability, a subset of subjects had a second PCSS screen by a second dental provider. Results The sample included 97 children (94%-Non-Hispanic-White, 4% Hispanic, 2% other; 49%-female), with a mean age of 8.6±2.2. PSQ-SRBD scores were elevated in 20 (20.6%) participants. Three children were reported to overweight (on item specific question of PSQ-SRBD scale). Children with elevated PSQ-SRBD (&amp;gt;0.33) had lower NSES compared to children with PSQ-SRBD scores &amp;lt; 0.33. PSQ-SRBD was associated with total PCSS score (OR=1.17 CI[1.01-1.36], p=0.033) and individual features including, narrow vaulted palate (OR=3.22, CI[0.94-10.93], p=0.03), forward head posture (OR=3.13, CI[1.11, 9.30], p=0.03), tongue thrust (OR=7.04, CI[1.20, 47.67], p=0.03), tongue tie (OR=7.58, CI[1.88, 33.58], p=0.005) and heart-shaped tongue (OR=11.21, CI[1.81, 98.11], p=0.01) after adjustment with age, sex, race and ethnicity. The correlation between total PCSS score and PSQ-SRBD was low (R=0.39, p= &amp;lt; 0.01), however in subjects with at least one positive craniofacial feature, the correlation between significant PCSS features and PSQ-SRBD was moderate (R=0.54, p= &amp;lt; 0.01). There was high inter-rater reliability (kappa=0.75) in a subset of 53 subjects with second PCSS screens. Conclusion Multiple craniofacial features assessed during routine dental visits were associated with increased risk for pediatric SDB. This study points to the utility of a craniofacial scale to assess SDB risk in children. This pilot study will be expanded to include a more diverse patient population. Support (if any)

Topic: Cleft Lip and Palate Research
Subfield: Genetics
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
MO761: Real-Time Prediction of Intradialytic Hypotension using Machine Learning and Cloud Computing Infrastructure

Abstract BACKGROUND AND AIMS Intradialytic hypotension (IDH) is a frequent complication of chronic hemodialysis that could lead to serious clinical outcomes and can be difficult to predict. We used machine learning (ML) and cloud computing infrastructure to develop and evaluate a model that enables real-time IDH prediction. METHOD Data were analyzed from six dialysis clinics (Fresenius Kidney Care, Waltham, MA, United States) between January 2020 and November 2020. Data included demographic, clinical, treatment and laboratory were obtained from the electronic health record. The intradialytic data such as systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate and blood flow rate were transferred in real time to Amazon Web Services (AWS, Amazon Web Services, Inc., Seattle, WA, United States) using secure Internet of Things software. Data were randomly divided into model training (80%) and testing (20%) sets. IDH was defined as SBP less than 90 mmHg. A ML tool known as an extreme gradient boosting algorithm (XGBoost) was used. Each time when an intradialytic SBP was measured, the model, which was hosted in AWS, would enable real-time prediction (in less than a minute) of patients who were at risk of IDH within the next 15–75 min. Area under the receiver operating characteristic curve (AUROC) was used to measure the model's performance. Shapley values were calculated to derive variables that were considered highly predictive of IDH. RESULTS We obtained data from 42 546 hemodialysis sessions involving 693 patients and 355 693 intradialytic observations. IDH occurred in 17% of the treatments. Our model reasonably predicted IDH, achieving an AUROC of 0.89. Top three predictors of IDH within the next 15–75 min were last intradialytic SBP, IDH rate of the last 10 treatments and mean nadir SBP of the last 10 treatments (Fig. 1). CONCLUSION Our study clearly shows the possibility of predicting IDH within the next 15–75 min using ML and cloud computing infrastructure, it suggests that the tool has the potential to assist clinicians to intervene proactively in patients at risk for IDH in real time.

Topic: Dialysis and Renal Disease Management
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Pengaruh Pengendalian Biaya, Fungsi Penjualan Terhadap Peningkatan Laba Perusahaan Bisnis Era Covid-19

Profit is the difference between revenue and total costs (implicit costs as well as explicit costs). Implicit costs include the opportunity costs incurred when the firm chooses to use a particular factor of production. Profit in accounting is the difference between the sales price and production costs. Profit is the element of most concern because the earnings figure is expected to be sufficient to represent the company's overall performance. Profits include three levels, namely: semantics, syntax and pragmatics. Then the sales target is to compare the profitability between products with different sizes and also the profitability between one sales area and another. This research focuses on cost control, sales function to increase PT Freeport Indonesia's profit. The population in this study are related units within PT Freeport Indonesia, namely the mining unit, production unit, warehouse unit, sales unit, accounting unit in Papua. These five units are part of the accounting-related department of PT Freeport Indonesia in Papua. The sample collection method is purposive sampling by taking samples from related units. The data analysis technique used in this study was SEM PLS. The results of this study indicate that cost control and sales functions have an effect on increasing the company profit in the Era Covid-19 of PT Freeport Indonesia.Laba adalah selisih antara pendapatan dengan total biaya (biaya implisit maupun biaya eksplisit). Biaya implisit termasuk biaya kesempatan yang terjadi ketika perusahaan memilih untuk menggunakan factor produksi tertentu. Laba dalam akuntansi adalah selisih antara harga penjualan dengan biaya produksi. Laba merupakan elemen yang paling menjadi perhatian karena angka laba diharapkan cukup untuk merepresentasi kinerja perusahaan secara keseluruhan. Laba meliputi tiga tataran, yaitu: semantik, sintaktik, dan pragmatik. Kemudian target penjualan adalah dengan membandingkan profitabilitas antar produk – produk dengan ukuran yang berbeda dan juga profitabilitas antara daerah penjualan yang satu dengan daerah pemasaran yang lainnya. Penelitian ini focus pada pengendalian biaya, fungsi penjualan untuk peningkatkan laba PT Freeport Indonesia. Populasi dalam penelitian ini adalah unit-unit terkait didalam PT Freeport Indonesia yaitu pada unit bagian tambang, unit produksi, unit gudang, unit penjualan, unit akuntansi di Papua. Kelima unit ini adalah bagian terkait akuntansi pada PT Freeport Indonesia di Papua. Metode pengumpulan sampel adalah purposive sampling dengan mengambil sampel pada pada unit terkait. Teknik analisis data yang digunakan didalam penelitian ini memakai SEM PLS. Hasil penelitian ini menunjukan bahwa pengendalian biaya, fungsi penjualan berpengaruh dalam peningkatkan laba perusahaan PT Freeport Indonesia Era Covid-19.

Topic: Management and Optimization Techniques
Subfield: Strategy and Management
Field: Business, Management and Accounting
Domain: Social Sciences
RIVALRY BETWEEN CHINA, USA AND RUSSIA IN GEOPOLITICS

Today, on the world stage, there is the concept of "Cold War 2.0", which, as usual, is characterized by the confrontation between the United States and China. However, there is also a third party in this conflict, which also takes an active part in global projects - the Russian Federation. In the 21st century, three powerful powers are trying to divide the sphere of their influence on the world stage, and each of them has its own goals and methods of achievement. This article will consider both points of rivalry between powers and points of cooperation, since these two concepts are close to each other.

Topic: Global Political and Economic Relations
Subfield: Political Science and International Relations
Field: Social Sciences
Domain: Social Sciences
Morphology of cells grown in the CFU-GEMM tissue culture assay from mononuclear cells obtained from peripheral blood and bone marrow of normal volunteers.

In ten healthy volunteers studies were done to assess the morphology, immunocytology, and cytochemical properties of mononuclear hematopoietic stem cells isolated from normal human peripheral blood and bone marrow grown in semisolid matrix mixed lineage colony-forming unit (CFU-GEMM) culture. In three volunteers, peripheral blood and bone marrow samples were collected simultaneously; the progeny were remarkably similar in each of the three paired cultures, although macrophages were more numerous in the bone marrow cultures. Three peripheral blood samples were cultured following frozen storage with 10% DMSO at-150 degrees C for approximately 5 months. Megakaryopoiesis was present in each case, demonstrating the full hematopoietic potential of previously frozen peripheral blood mononuclear cells. Four other peripheral blood samples and one bone marrow sample were cultured and, in each case, cells of all hematopoietic cell lines were present. Bone marrow, peripheral blood mononuclear cells, and previously frozen peripheral blood mononuclear cells grown in the CFU-GEMM tissue culture assay showed the presence of granulocyte-macrophage, erythroid, and megakaryocytic cell lines.

Topic: Hematopoietic Stem Cell Transplantation
Subfield: Hematology
Field: Medicine
Domain: Health Sciences
In-home family-focused reunification: A six-year follow-up of a successful experiment

The services provided to 120 children (62 experimental and 58 control) by state welfare agencies were followed for six years. When identified initially, all of the children were in out-of-home placements. After a 12-month follow-up of an experimental 90-day intensive in-home, family-based intervention, 75 percent of the children in the experimental group were reunified with their families, compared with 49 percent of the children in the control group who received routine child welfare services. Over six years, using state computer databases, it was determined that the children in the experimental group required less supervision time, lived at home longer, and were in less-restrictive placements than those in the control group. At the time all public agency involvement was terminated, two-thirds of the experimental families were classified as “stabilized,” compared with approximately one-third of the control group. The experimental treatment had a substantial effect on families, which continued throughout the six-year follow-up period.

Topic: Homelessness and Social Issues
Subfield: General Health Professions
Field: Health Professions
Domain: Health Sciences
Prevalence of malocclusion in public school students in the mixed dentition phase and its association with early loss of deciduous teeth

ABSTRACT Objective: To determine the prevalence of malocclusion and its association with the early loss of deciduous teeth and other factors in children in the mixed dentition phase, aged six to eight years, enrolled in public schools in southern Brazil. Methods: A cross-sectional study was conducted with a representative sample of 528 children from municipal public schools in 2009. Data collection involved a clinical examination for the determination of early tooth loss, dental caries, tongue pressure and malocclusion (outcome), as well as the administration of a questionnaire. Poisson regression analysis with robust variance was used to model the association between malocclusion and the independent variables. Results: The prevalence of malocclusion and early tooth loss was 69.1% and 21.8%, respectively. In the raw data analysis, malocclusion was associated with age, early tooth loss, dental caries and tongue pressure. After the adjustment, the likelihood of malocclusion was greater among children older than eight years, those who exerted tongue pressure on the teeth and those with early tooth loss. The likelihood of malocclusion was 24% greater among children with early tooth loss, compared to those without tooth loss. Conclusion: The early loss of deciduous teeth was associated with the occurrence of malocclusion in the children studied.

Topic: Engineering Technology and Methodologies
Subfield: Industrial and Manufacturing Engineering
Field: Engineering
Domain: Physical Sciences
The Safety of Amiodarone for Heart Failure Patients with Atrial Fibrillation

Background: The purpose of this study was to investigate the difference of the safety of oral amiodarone in heart failure patients by presence of atrial fibrillation. Methods: The subjects were 75 patients in whom the oral amiodarone was administrated for the treatment of tachyarrhythmia and with anamnesis of heart failure. We compared the background, the frequency of side effect and taking period between group 1 (with atrila fibrillation, n=46) and group 2 (without atrial fibrillation, n=29). Results: In Group 1, significantly age (69±7 vs. 60±13 years old: p<0.0001) was high and diameter of LA (47±10 vs. 40±6 mm: p<0.0001) was large. But there was no significant difference of LV ejection fractions (mean 34±11%), diameter of LV (mean 60±9 mm), ratio of taking β-blocker (84%), ACE/ARB (79%), dose of amiodarone (mean 150±60 mg/day), and taking period (mean 31±35 months: 0.1–183 months) between two groups. The frequency of side effect was 9% (4/46) in group 1 and 17% (5/29) in group 2, there was no significant difference. The side effect were three interstitial pneumonia (4%), one respiratory symptom, one pancytopenia, one eosinophilia, one bradycardia, one liver dysfunction, one hypothyroidism. Conclusion: There was no difference of the frequency of side effect and taking period of oral amiodarone in heart failure patients by presence of atrial fibrillation.

Topic: Cardiac electrophysiology and arrhythmias
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
[Study on the clinical significance of plasma adrenomedullin and brain natriuretic polypeptide in the patients with chronic cor pulmonale on highland].

The study suggests that ADM and BNP are involved in the pathophysiological process of HACCP in the patients and may play a compensatory role in the disease.

Topic: Neonatal Respiratory Health Research
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Improved Extraction of Saturated Fatty Acids but not Omega‐3 Fatty Acids from Sheep Red Blood Cells Using a One‐Step Extraction Procedure

Abstract Several methods are available to extract total lipid and methylate fatty acids from a range of samples including red blood cells (RBC). Fatty acid analysis of human RBC can be undertaken using a two‐step extraction and methylation or a combined one‐step extraction and methylation procedure. The lipid composition of sheep RBC differs significantly from that of humans and may affect their extraction. The purpose of the current study was to examine the efficiency of extraction of lipid and detection of fatty acids from sheep RBC using a one‐step procedure. Fatty acids were analysed using a one‐step extraction and methylation procedure using methanol:toluene and acetyl chloride in comparison with a two‐step procedure involving extraction of lipid using chloroform:methanol and separate methylation. Concentrations of saturated fatty acids including C16:0 and C18:0 were significantly higher (42.6 and 33.9 % respectively) following extraction using the one‐step procedure compared with the two‐step procedure. However, concentrations of some polyunsaturated fatty acids, including C20:5n‐3 and C22:6n‐3 were not significantly different between either procedure. The improved detection of fatty acids may be related to the ability of different solvents to extract different lipid fractions. The differential extraction of lipids and detection of fatty acids from sheep RBC may have important implications in studies examining the effect of dietary treatment on the possible health benefits of fatty acids.

Topic: Fatty Acid Research and Health
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
The Study on Quality Evaluation System of Graduate Education in the Era of Big Data

This paper proposes to carry out the quality evaluation of graduate education based on big data technology and construct the quality evaluation index system of graduate education based on big data. It also proposes to use modern information technology to continuously collect and deeply analyze the relevant data in the graduate education system, intuitively present the state of graduate education, and provide objective basis for multi subject value judgment and scientific decision-making.

Topic: Big Data Technologies and Applications
Subfield: Information Systems and Management
Field: Decision Sciences
Domain: Social Sciences
Role of carrier-based biofertilizer in reclamation of saline soil and wheat growth

Two plant growth promoting rhizobacteria (PGPR), Pseudomonas moraviensis and Bacillus cereus, were used as bioinoculants on wheat, applied alone and in combination. Ground maize straw and sugarcane husk were used as carriers. Experiment was conducted for two consecutive years (2010 and 2011) under axenic conditions in the greenhouse of Quaid-e-Azam University, Islamabad. Sodium chloride (NaCl) (150 mM) was applied with irrigated water after 7 and 14 days of seed germination. Measurements made 40 days after sowing (DAS) revealed that P. moraviensis and B. cereus have better survival efficiency (as evidenced by higher colony forming units (CFUs)) in the carriers. The substantial increase in CFU of both PGPR was also observed in the soil at 57 DAS. Coinoculation of PGPR with both the carrier materials significantly decreased electrical conductivity (EC) and Na+ content of soil over control. The N, P, K+, Ca+, and Mg+ contents were 30–40% higher in soil, and 30–45% higher in leaves. Coinoculation of PGPR with carriers significantly increased chlorophyll, protein, sugar, phytohormone contents, and antioxidant activities of leaves. The application of biofertilizers improved the yield of wheat by 15–25% over control. It is inferred that the carriers assisted PGPR for long-time survival, and the formulation was applicable in promoting crop production under salt stress.

Topic: Plant-Microbe Interactions and Immunity
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences