575 NANOTECHNOLOGY PSA MEASUREMENTS CORRELATE WITH PROSTATE CANCER PATHOLOGY AND RESPONSE TO ADJUVANT TREATMENT

You have accessJournal of UrologyProstate Cancer: Advanced I1 Apr 2010575 NANOTECHNOLOGY PSA MEASUREMENTS CORRELATE WITH PROSTATE CANCER PATHOLOGY AND RESPONSE TO ADJUVANT TREATMENT Lee C. Zhao, Dae Y. Kim, Brian Le, Hannah Alphs, Brian T. Helfand, Donghui Kan, Norm D. Smith, Chad A. Mirkin, C. Shad Thaxton, Stacy Loeb, and William J. Catalona Lee C. ZhaoLee C. Zhao Chicago, IL More articles by this author , Dae Y. KimDae Y. Kim Chicago, IL More articles by this author , Brian LeBrian Le Chicago, IL More articles by this author , Hannah AlphsHannah Alphs Chicago, IL More articles by this author , Brian T. HelfandBrian T. Helfand Chicago, IL More articles by this author , Donghui KanDonghui Kan Chicago, IL More articles by this author , Norm D. SmithNorm D. Smith Chicago, IL More articles by this author , Chad A. MirkinChad A. Mirkin Evanston, IL More articles by this author , C. Shad ThaxtonC. Shad Thaxton Chicago, IL More articles by this author , Stacy LoebStacy Loeb Baltimore, MD More articles by this author , and William J. CatalonaWilliam J. Catalona Chicago, IL More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.834AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We recently described a nanotechnology-enabled PSA research-use-only assay (nPSA) that is 2 orders of magnitude more sensitive for the detection of PSA than conventional PSA immunoassays (cPSA). A potential clinical application of such an assay is the early detection of biochemical progression after radical prostatectomy (RP) and the subsequent monitoring of secondary treatment response. Accordingly, by using a commercial nPSA assay (Nanosphere, Inc.), we sought to evaluate the nPSA profiles in patients with favorable pathologic characteristics and those with unfavorable characteristics at RP, including men who underwent adjuvant radiation treatment (XRT). METHODS The nPSA assay was used in 188 prospectively banked serum specimens from patients with cPSA values ≤0.1 ng/mL following RP. Initial nPSA values following treatment (RP ± adjuvant XRT) were compared between patients with favorable versus unfavorable pathologic characteristics, and in those with unfavorable pathology who underwent immediate XRT. Favorable characteristics were defined as: organ-confined disease, Gleason <4+3=7, and negative surgical margins. Unfavorable disease was defined as extracapsular tumor extension with positive surgical margins, seminal vesicle invasion, lymph node metastases, or a Gleason grade ≥4+3=7. RESULTS Initial nPSA values were significantly lower for patients with favorable versus unfavorable pathology with a mean value of 5.8 vs. 10.1 pg/mL (p=0.016, Table). Further, patients with unfavorable pathology who received immediate XRT had a significantly lower mean nPSA (1.7 vs. 10.1 pg/mL, p<0.001). Table 1. Post-Treatment nPSA Levels (pg/mL) Mean Med Min Max Favorable (n=70) 5.8 3.8 <0.5 36.8 Unfavorable without adjuvant XRT (n=98) 10.1 3.8 <0.5 91.6 Unfavorable with adjuvant XRT (n = 20) 1.7 1 <0.5 6.4 CONCLUSIONS Among post-prostatectomy patients with an undetectable cPSA, initial nPSA measurements were higher in those with adverse pathology. The finding of lower nPSA among patients with adverse features who received adjuvant XRT suggests a pelvic source of PSA in these cases. These results suggest that nPSA may be used to stratify patients according to their risk of cancer recurrence and to quantify their response to adjuvant therapy. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e225-e226 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Lee C. Zhao Chicago, IL More articles by this author Dae Y. Kim Chicago, IL More articles by this author Brian Le Chicago, IL More articles by this author Hannah Alphs Chicago, IL More articles by this author Brian T. Helfand Chicago, IL More articles by this author Donghui Kan Chicago, IL More articles by this author Norm D. Smith Chicago, IL More articles by this author Chad A. Mirkin Evanston, IL More articles by this author C. Shad Thaxton Chicago, IL More articles by this author Stacy Loeb Baltimore, MD More articles by this author William J. Catalona Chicago, IL More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

Topic: Advanced biosensing and bioanalysis techniques
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences