jimnoneill/paper-to-field-training huggingface dataset:
Functions of Yeast Hsp40 Chaperone Sis1p Dispensable for Prion Propagation but Important for Prion Curing and Protection From Prion Toxicity

Abstract Replication of amyloid-based yeast prions [PSI+], [URE3], and [PIN+] depends on the protein disaggregation machinery that includes Hsp104, Hsp70, and Hsp40 molecular chaperones. Yet, overexpressing Hsp104 cures cells of [PSI+] prions. An Hsp70 mutant (Ssa1-21p) antagonizes propagation of [PSI+] in a manner resembling elevated Hsp104. The major cytosolic Hsp40 Sis1p is the only Hsp40 required for replication of these prions, but its role in [PSI+] curing is unknown. Here we find that all nonessential functional regions of Sis1p are dispensable for [PSI+] propagation, suggesting that other Hsp40’s might provide Hsp40 functions required for [PSI+] replication. Conversely, several Sis1p functions were important for promoting antiprion effects of both Ssa1-21p and Hsp104, which implies a link between the antiprion effects of these chaperones and suggests that Sis1p is a specific Hsp40 important for [PSI+] curing. These contrasting findings suggest that the functions of Hsp104 that are important for propagation and elimination of [PSI+] are either distinct or specified by different Hsp40's. This work also uncovered a growth inhibition caused by [PSI+] when certain functions of Sis1p were absent, suggesting that Sis1p protects cells from cytotoxicity caused by [PSI+] prions.

Topic: Prion Diseases and Protein Misfolding
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
[Factors affecting nosocomial infection in the surgery setting at the Hospital of Point "G"].

Nosocomial infections are a common and increasing problem globally, and particularly in Africa. The significant economic burden of these infections on the health care system as well as their impact on patient morbidity and mortality is well recognised within the medical communities.The goal of this study was to investigate factors that influence nosocomial infection in all in-patients from the surgery wards (surgery ward A and B, Gynoeco-obstetrics, urology and intensive care) at the national hospital of Point G in Bamako, Mali. We report the number of nosocomial infection during a prospective study between June 2003 and January 2004. Of 1043 in-patients, 102 had had a nosocomial infection with a global prevalence of 9.7% (8.0-11.4). Of 1024 patients with surgery, 101 had had a nosocomial infection, a post-surgery nosocomial infection rate of 9.8% (8.1-11.5). We observed different site of nosocomial infection such as suppuration sup-aponevrotics (41.2%), parietal infection sub-aponevrotics (32.4%), urinary infection (17.6%) and organ suppuration (3.9%). Other nosocomial infection observed were pneumonia (2.9%) and catheterisation (2.0%). The nosocomial infection rate was 10.3% in male while it was 7.3% in female. The difference between male and female was not statistically significative (chi2 = 2.33, p = 0.12). Nosocomial infection was more prevalent in patients after emergency surgery (15.1%) than in scheduled surgery patients (8.5%) (chi2 = 8.15, p = 0.004). The classes III and IV of ALTEMEIER had the higher proportion of nosocomial infection (35.9%) against 4.8% for the classes I and II (chi2 = 144.95, p < 0.001). The patients with ASA score I had a lower nosocomial infection rate than patients from the intensive care unit or patients of Class II + III + IV (chi2 = 13.2, p = 0.001). Patients classified according to the National Nosocomial Infection Surveillance System (NNISS) with a score 0 had a nosocomial infection rate less than patients classified as NNISS score 1, 2 or 3 (chi2 = 82.0, p < 0.001). The study results underline the need for further investigations of the role of microbial agents and antimicrobial resistance in the outcome of patients with nosocomial infection.

Topic: Healthcare Systems and Practices
Subfield: General Health Professions
Field: Health Professions
Domain: Health Sciences
Screening, Recruitment, and Baseline Characteristics for the Strategies to Reduce Injuries and Develop Confidence in Elders (STRIDE) Study

BACKGROUND: We describe the recruitment of participants for Strategies to Reduce Injuries and Develop Confidence in Elders (STRIDE), a large pragmatic cluster randomized trial that is testing the effectiveness of a multifactorial intervention to prevent serious fall injuries. Eligible persons were 70 years or older, community-living, and at increased risk for serious fall injuries. The modified goal was to recruit 5,322 participants over 20 months from 86 primary care practices within 10 diverse health care systems across the United States. METHODS: The at-risk population was identified using two distinct but complementary screening strategies that included three questions administered centrally via the mail (nine sites) or in the clinic (one site), while recruitment was completed centrally by staff at Yale. RESULTS: For central screening, 226,603 letters mailed to 135,118 patients yielded 28,719 positive screens (12.7% of those mailed and 46.5% of the 61,729 returned). In the clinic, 22,537 screens were completed, leading to 5,732 positive screens (25.4%). Of the 34,451 patients who screened positive for high risk of serious fall injuries, 31,872 were sent a recruitment packet and, of these, 5,451 (17.1%) were enrolled over 20 months (mean age: 80 years; 62% female). The participation rate was 34.0% among eligible patients. The enrollment yields were 3.6% (vs 5% projected) for each patient screened centrally, despite multiple screens, and 10.5% (vs 33.9% projected) for each positive clinic screen. CONCLUSIONS: Despite lower-than-expected yields, the STRIDE Study exceeded its modified recruitment goal. If the STRIDE intervention is found to be effective, the two distinct strategies for identifying a high-risk population of older persons could be implemented by most health care systems.

Topic: Balance, Gait, and Falls Prevention
Subfield: Physical Therapy, Sports Therapy and Rehabilitation
Field: Health Professions
Domain: Health Sciences
Prospective study of germline and somatic alterations for early onset lung cancer patients (EOLUNG MASTER protocol).

TPS9122 Background: Lung cancer (LC) is a rare disease among young adults. Polymorphisms in genes involved in carcinogenic metabolism of tobacco and/or xenobiotic metabolism have been identified in those patients. The growing use of next generation sequencing (NGS) unravelled germline mutations in genes associated with hereditary cancer in patients with LC. However, germline DNA mutations have not been systematically studied in young adults with LC. Early onset LC patients are likely to harbor actionable somatic mutations. Broad hybrid NGS can identify actionable genomic alterations in LC patients deemed driver negative by routine molecular analysis. Trial Design: This is a multicentre, prospective, single-cohort study to determine whether young patients with LC harbor germline mutations and to evaluate the impact of NGS using Foundation One platform on the therapeutic options and overall survival for this specific patient population. Major inclusion criteria: 1) patients already or newly diagnosed of non-small cell lung cancer at any stage at age &lt; 51; 2) to have sufficient tumor sample to perform standard molecular diagnosis ( EGFR/ALK/ROS1/BRAF); 3) to provide written informed consent for the study. Methods: After signing the informed consent form, family history of cancer and smoking history will be collected and a peripheral blood sample will be obtained. Germline targeted sequencing will be carried out in our centre using a customized hereditary cancer panel (I2HCP) of 136 genes designed to cover the coding exons and intron-exon boundaries of genes associated with moderate or high risk of hereditary cancer. Patients with clinical criteria for hereditary cancer or harboring pathogenic or probably pathogenic germline alteration will be referred to the genetic counselling unit. Patients will follow the usual clinical pathway for biomarker analysis and in case of remaining tumour material it will be used for conducting comprehensive genomic profiling through Foundation One platform. When tumour material available will not be sufficient to perform NGS, a tumour re-biopsy will be considered. Enrolment begun on June 2018 and, to date, a total of 41 patients have been included in the study.

Topic: Cancer Genomics and Diagnostics
Subfield: Cancer Research
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Peripheral blood leucocyte subpopulations in patients splenectomized for trauma.

Monoclonal antibodies were used to type leucocyte subpopulations in peripheral blood mononuclear leucocytes (MNL) from patients who had had spleens removed following trauma. The proportion of OKT3+ (T) cell and OKT4+ ('T helper/inducer') cell was significantly decreased in splenectomized subjects. While a decrease was also observed for the OKT8+ ('T suppressor/cytotoxic') cells, this was not significant. The ratio of OKT4+/OKT8+ cells was significantly decreased in the splenectomized group. Interestingly, the absolute numbers of OKT3+, OKT4+ and OKT8+ cells were increased. The % of B lymphocytes (identified as Ig+ and FMC1+ cells) was significantly increased in patients. The proportion of MHC Class II+ cells (FMC4+) was also increased although not significantly. A marked increase in % of monocytes (FMC33+) was observed in patients. The changes in proportion of regulatory T cells and monocytes may in part explain the depressed mitogen responses of MNL from splenectomized subjects.

Topic: Abdominal Trauma and Injuries
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Circulating SOD2 Is a Candidate Response Biomarker for Neoadjuvant Therapy in Breast Cancer

There is a great need for non-invasive tools that inform of an early molecular response to cancer therapeutic treatment. Here, we tested the hypothesis that proteolytically resistant proteins could be candidate circulating tumor biomarkers for cancer therapy. Proteins resistant to proteolysis are drastically under-sampled by current proteomic workflows. These proteins could be reliable sensors for the response to therapy since they are likely to stay longer in circulation. We selected manganese superoxide dismutase (SOD2), a mitochondrial redox enzyme, from a screening of proteolytic resistant proteins in breast cancer (BC). First, we confirmed the robustness of SOD2 and determined that its proteolytic resistance is mediated by its quaternary protein structure. We also proved that the release of SOD2 upon chemotherapy treatment correlates with cell death in BC cells. Then, after confirming that SOD2 is very stable in human serum, we sought to measure its circulating levels in a cohort of BC patients undergoing neoadjuvant therapy. The results showed that circulating levels of SOD2 increased when patients responded to the treatment according to the tumor shrinkage during neoadjuvant chemotherapy. Therefore, the measurement of SOD2 levels in plasma could improve the non-invasive monitoring of the therapeutic treatment in breast cancer patients. The identification of circulating biomarkers linked to the tumor cell death induced by treatment could be useful for monitoring the action of the large number of cancer drugs currently used in clinics. We envision that our approach could help uncover candidate tumor biomarkers to measure a tumor’s response to cancer therapy in real time by sampling the tumor throughout the course of treatment.

Topic: Advanced Proteomics Techniques and Applications
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
POM of 2326 archetypes in Maven central repository

This dataset contains <em>pom.xml </em>files of 2326 archetypes in Maven central repository. The format of file name of each <em>pom.xml</em> is "&lt;groupId&gt;=&lt;artifactId&gt;=&lt;version&gt;=&lt;orginal file name&gt;", where "." in groupId is replace as "=". For example, the file name "am=ik=archetype=spring-boot-blank-archetype=0.9.0=spring-boot-blank-archetype-0.9.0.pom" represents: groupId: am.ik.archetype artifactId: spring-boot-blank-archetype version: 0.9.0 pom.xml original filename: spring-boot-blank-archetype-0.9.0.pom

Topic: Ancient Egypt and Archaeology
Subfield: Archeology
Field: Arts and Humanities
Domain: Social Sciences
PALAEOGRAPHY AND ITS USES

PALAEOGRAPHY AND ITS USES Get access A. SOUTER A. SOUTER Search for other works by this author on: Oxford Academic Google Scholar The Journal of Theological Studies, Volume os-IV, Issue 16, July 1903, Pages 506–516, https://doi.org/10.1093/jts/os-IV.16.506 Published: 01 July 1903

Topic: Historical, Religious, and Philosophical Studies
Subfield: Archeology
Field: Arts and Humanities
Domain: Social Sciences
Silver Nanoparticles Conjugate Attenuates Highly Active Antiretroviral Therapy-Induced Hippocampal Nissl Substance and Cognitive Deficits in Diabetic Rats

Background. The application of nanomedicine to antiretroviral drug delivery holds promise in reducing the comorbidities related to long-term systemic exposure to highly active antiretroviral therapy (HAART). However, the safety of drugs loaded with silver nanoparticles has been debatable. This study is aimed at evaluating the effects of HAART-loaded silver nanoparticles (HAART-AgNPs) on the behavioural assessment, biochemical indices, morphological, and morphometric of the hippocampus in diabetic Sprague-Dawley rats. Methods. Conjugated HAART-AgNPs were characterized using FTIR spectroscopy, UV spectrophotometer, HR-TEM, SEM, and EDX for absorbance peaks, size and morphology, and elemental components. Forty-eight male SD rats ( <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mn>250</a:mn> <a:mo>±</a:mo> <a:mn>13</a:mn> </a:math> g) were divided into nondiabetic and diabetic groups. Each group was subdivided into ( <c:math xmlns:c="http://www.w3.org/1998/Math/MathML" id="M2"> <c:mi>n</c:mi> <c:mo>=</c:mo> <c:mn>8</c:mn> </c:math> ) A (nondiabetic+vehicle), B (nondiabetic+HAART), C (nondiabetic+HAART-AgNPs), D (diabetic+vehicle), E (diabetic+HAART), and F (diabetic+HAART-AgNPs). Morris water maze, Y-maze test, and weekly blood glucose levels were carried out. Following the last dose of 8-week treatment, the rats were anaesthetized and euthanized. Brain tissues were carefully removed and postfixed for Nissl staining histology. Results. 1.5 M concentration of HAART-AgNPs showed nanoparticle size 20.3 nm with spherical shape. HAART-AgNPs revealed 16.89% of silver and other elemental components of HAART. The diabetic control rats showed a significant increase in blood glucose, reduced spatial learning, positive hippocampal Nissl-stained cells, and a significant decrease in GSH and SOD levels. However, administration of HAART-AgNPs to diabetic rats significantly reduced blood glucose level, improved spatial learning, biochemical indices, and enhanced memory compared to diabetic control. Interestingly, diabetic HAART-AgNP-treated rats showed a significantly improved memory, increased GSH, SOD, and number of positive Nissl-stained neurons compared to diabetic-treated HAART only. Conclusion. Administration of HAART to diabetic rats aggravates the complications of diabetes and promotes neurotoxic effects on the experimental rats, while HAART-loaded silver nanoparticle (HAART-AgNP) alleviates diabetes-induced neurotoxicity.

Topic: HIV-related health complications and treatments
Subfield: Emergency Medicine
Field: Medicine
Domain: Health Sciences
A contribution to the taxonomy, cytogenetics and reproductive biology of the genus Aclerda Signoret (Homoptera, Coccinea, Aclerdidae)

This dataset contains the digitized treatments in Plazi based on the original journal article Gavrilov-Zimin, I. A. (2012): A contribution to the taxonomy, cytogenetics and reproductive biology of the genus Aclerda Signoret (Homoptera, Coccinea, Aclerdidae). CompCytogen 6: 389-395, DOI: http://dx.doi.org/10.3897/CompCytogen.v6i4.4320, URL: http://dx.doi.org/10.3897/CompCytogen.v6i4.4320

Topic: Research on scale insects
Subfield: Insect Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Effects of Different Tillage Measures on Soil Microbes and Enzymatic Activity

To reveal the effects of different tillage measures on soil microbes, enzyme activities and nutrients, eight different treatments were combined with different tillage methods to study the effects of different tillage measures on soil microbes, enzyme activities and nutrients. The results showed that in the flowering stage, the number of bacteria in the soil was higher than that in PT1 (ploughing + early ridging), RT1 (rotary tillage + early ridge) decreased by 22.6%. PT2 (ploughing + early subsoiling) was more than RT2 (rotary tillage + early subsoiling), PT3 (ploughing + conventional subsoiling) than RT3 (rotary tillage + conventional subsoiling), PCK (rotary tillage + conventional ridging) than RCK (rotary tillage + conventional ridging) increased by 13%, 22.9%, and 3.5%, respectively. The bacteria, fungi and actinomycetes in the whole rotary tillage treatment (rotary tillage) were higher than the tillage treatment (ploughing). Except for the invertase, the urease, phosphatase and catalase activities of the ploughing treatment were higher than those of the rotary tillage. When entering the pod-forming period, the number of soil bacteria, fungi and actinomycetes in rotary tillage were higher than tillage, the soil sucrase activity in rotary tillage was higher than that in ploughing. RT1 was significantly higher than PT1 and PT2 by 11.1% and 11.7%, but the soil urease, phosphatase and catalase activities were higher in ploughing than rotary tillage. The available nitrogen and the available potassium were opposite. At the stage of tillage, the number of bacteria, fungi, actinomycetes, urease activities and sucrase activities in the rotary tillage were higher than ploughing, while the activities of phosphatase and catalase were higher under ploughing. In the mature stage, the soil urease and catalase activities were higher in the tillage treatment, while the phosphatase and sucrase activities were opposite. On the whole, advance inter-tillage improved the soil environment to some extent on both tillage measures, especially T2 (subsoiling).

Topic: Soil Carbon and Nitrogen Dynamics
Subfield: Soil Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
The hinge‐1 domain of Flna is not necessary for diverse physiological functions in mice

Abstract Introduction The filamins are cytoskeletal binding proteins that dynamically crosslink actin into orthogonal networks or bundle it into stress fibres. The domain structure of filamin proteins is very well characterised, with an N‐terminal actin‐binding region, followed by 24 immunoglobulin‐like repeat units. The repeat domains are separated into distinct segments by two regions of low‐complexity known as hinge‐1 and hinge‐2. The role of hinge‐1 especially has been proposed to be essential for protein function as it provides flexibility to the otherwise rigid protein, and is a target for cleavage by calpain. Hinge‐1 protects cells from otherwise destructive forces, and the products of calpain cleavage are involved in critical cellular signalling processes, such as survival during hypoxia. Pathogenic variants in FLNA encoding Filamin A, including those that remove the hinge‐1 domain, cause a wide range of survivable developmental disorders. In contrast, complete loss of function of this gene is embryonic lethal in human and mouse. Methods and Results In this study, we show that removing filamin A hinge‐1 from mouse ( Flna ΔH1 ), while preserving its expression level leads to no obvious developmental phenotype. Detailed characterisation of the skeletons of Flna ΔH1 mice showed no skeletal phenotype reminiscent of that found in the FLNA‐ causing skeletal dysplasia. Furthermore, nuclear functions of FLNA are maintained with loss of Filamin A hinge‐1. Conclusion We conclude that hinge‐1 is dispensable for filamin A protein function during development over the murine lifespan.

Topic: Calpain Protease Function and Regulation
Subfield: Cell Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Nursing FORM) 2023(2024,Nana College of Nursing Sciences, Anyingba ADMISSION Form is out call (O8O3664O134 or O8O3664O134) Post-Basic Nursing form, Midwifery  candidates must fulfil the following criteria: Candidates Call O8O3664O134 must possess five credits (Mathematics, English, Chemistry, Biology, Physics) in WAEC, NECO, NABTEB or a combination of WAEC and NECO. Please come with a photocopy of your results. All candidates should submit a handwritten application letter addressed to the Principal of the Candidates are required to provide four recent passport photographs.

Nursing FORM) 2023(2024,Nana College of Nursing Sciences, Anyingba ADMISSION Form is out call (O8O3664O134 or O8O3664O134) Post-Basic Nursing form, Midwifery candidates must fulfil the following criteria: Candidates Call O8O3664O134 must possess five credits (Mathematics, English, Chemistry, Biology, Physics) in WAEC, NECO, NABTEB or a combination of WAEC and NECO. Please come with a photocopy of your results. All candidates should submit a handwritten application letter addressed to the Principal of the Candidates are required to provide four recent passport photographs.

Topic: Hermeneutics and Narrative Identity
Subfield: Philosophy
Field: Arts and Humanities
Domain: Social Sciences
Diverging effects of subjective prospect values of uncertain time and money

Studies from behavioral economics show that the subjective prospect value of money has diminishing sensitivity to losses/gains, represented by an S-shape, and this has been applied in representing the subjective prospect value of time in many transportation studies such as travel behavior modeling and network equilibrium. In this study, we demonstrate that the prospect value of time has an increasing sensitivity to losses/gains and can be represented by an Ϩ-shape, which contrasts that of money. We further explain the rationality of this surprising finding based on psychological and behavioral theories and discuss extensive practical implications. The correlations between sensitivities to gains and losses in terms of magnitude are revealed as well to shed light on potential underlying correlated behavioral principles. Substantial loss-aversion features are observed in the empirical analysis, supporting endowment effects. Implications of the findings on decision-making and other areas that utilize time as a key indicator have been discussed. The findings may revolutionize many research areas that utilize time as a key indicator such as transportation engineering.

Topic: Economic and Environmental Valuation
Subfield: Economics and Econometrics
Field: Economics, Econometrics and Finance
Domain: Social Sciences