jimnoneill/paper-to-field-training huggingface dataset:
Immunogenicity of a Bovine Herpesvirus 1 Glycoprotein D DNA Vaccine Complexed with Bovine Neutrophil Beta-Defensin 3

ABSTRACT Protective efficacy against bovine herpesvirus 1 (BoHV-1) has been demonstrated to be induced by a plasmid encoding bovine neutrophil beta-defensin 3 (BNBD3) as a fusion construct with truncated glycoprotein D (tgD). However, in spite of the increased cell-mediated immune responses induced by this DNA vaccine, the clinical responses of BoHV-1-challenged cattle were not reduced over those observed in animals vaccinated with the plasmid encoding tgD alone; this might have been because the vaccine failed to improve humoral responses. We hypothesized that an alternative vaccine design strategy that utilized the DNA vaccine pMASIA-tgD as a complex with BNBD3 might improve humoral responses while maintaining robust Th1-type cell-mediated responses. C57BL/6 mice were vaccinated with pMASIA-tgD complexed with 0, 0.01875, 0.1875, or 1.875 nmol of a stable synthesized analog of BNBD3 (aBNBD3). The best results were seen in mice immunized with the vaccine composed of pMASIA-tgD complexed to 0.1875 nmol aBNBD3. In this group, humoral responses were improved, as evidenced by increased virus neutralization, tgD-specific early IgG1, and later IgG2a titers, while the strong cell-mediated immune responses, measured based on specific gamma interferon (IFN-γ)-secreting cells, were maintained relative to pMASIA-tgD. Modulation of the immune response might have been due in part to the effect of BNBD3 on dendritic cells (DCs). In vitro studies showed that murine bone marrow-derived DCs (BMDCs) pretreated with aBNBD3 were activated, as evidenced by CD11c downregulation, and were functionally mature, as shown by increased allostimulatory ability. Native, synthetic, and analog forms of BNBD3 were equally capable of inducing functional maturation of BMDCs.

Topic: Antimicrobial Peptides and Activities
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
DETERMINING KINETIC PARAMETERS FOR THERMAL INACTIVATION OF HEATRESISTANT AND HEAT‐ LABILE ISOZYMES FROM THERMAL DESTRUCTION CURVES

ABSTRACT A simple method is proposed to analyze the kinetics of thermal inactivation of enzyme systems which consist of two groups differing in their thermal stability. Using this approach kinetic parameters for thermal destruction for each fraction (the heat labile and heat resistant fraction) can be estimated directly from thermal treatment data. However, to determine the relative concentration of the fractions within the enzyme system, additional information concerning the enzyme assay used to determine enzyme activity is required. Commercially available horseradish peroxidase was used to test the applicability of the method. Analysis of data generated from thermal treatments of the peroxidase using the proposed method were found to be satisfactory. E a values of 21 kcal/mole and 34 kcal/mole were obtained for the heat resistant and heat labile horseradish peroxidase isozymes, respectively. D 82c values were 42 min and 1.2 min, respectively.

Topic: Enzyme-mediated dye degradation
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Diffraction efficiency of bacteriorhodopsin films for holography containing bacteriorhodopsin wildtype BRWT and its variants BRD85E and BRD96N

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDiffraction efficiency of bacteriorhodopsin films for holography containing bacteriorhodopsin wildtype BRWT and its variants BRD85E and BRD96NNorbert Hampp, Andreas Popp, Christoph Braeuchle, and Dieter OesterheltCite this: J. Phys. Chem. 1992, 96, 11, 4679–4685Publication Date (Print):May 1, 1992Publication History Published online1 May 2002Published inissue 1 May 1992https://pubs.acs.org/doi/10.1021/j100190a098https://doi.org/10.1021/j100190a098research-articleACS PublicationsRequest reuse permissionsArticle Views105Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts

Topic: Photoreceptor and optogenetics research
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Fingerprint Database Optimization Method for Indoor Localization Based on Neighbor Mean Filter

Wi-Fi is widely used for network communication in our daily life here and there. In addition to communication, it is also the reference information that is widely used in indoor positioning, such as localization based on RSSI (Radio Signal Strength Indicator) fingerprint database and range based localization. However, the broadcasting signals from wireless access point suffers from the complex indoor environment, such as multi-path fading and non-homogeneous environment. And the received signals' strength at the mobile terminal is instable, which must influence the positioning precision in indoor localization. Although these factors are existed, localization based on RSSI fingerprint database are still used widely indoors. There are 2 stages in the RSSI fingerprint database localization, offline fingerprint database building and online positioning. And the quality of both fingerprint database and real time RSSI values influence the precision of localization. However, the RSSI based indoor localization algorithms existed are almost conducted on the original fingerprint database which is not optimized. Some abnormal RSSI values in fingerprint database must destroy the distribution of the RSSI road map and result in low precision. In order to decrease the influence of original data containing bizarre RSSI possibly, filtering technology should be applied for the database. Considering the geographic correlation among RSSI values in road map, the neighbor mean filter (NMF) is introduced to optimize the fingerprint database in this paper. The idea of NMF is simply to replace each pixel value in an image with the mean value of its neighbors, excluding itself. Thus, it has the advantage for filtering the bizarre RSSI values in fingerprint database. In the paper, the introduction of NMF algorithm is described firstly, then, the bizarre value of RSSI fingerprint database is analyzed and the optimization on fingerprint database with NMF is narrated, especially, the method of determining threshold for optimization. Second, the indoor localization with optimized fingerprint database is put forward, which includes the architecture for RSSI fingerprint database localization, cosine calculation of RSSI vectors and their similarity judgement, and the localization based on optimized fingerprint database. Through these measures, the abnormal RSSI values found by NMF method and replaced with mean RSSI values could make the fingerprint database more accurate for the indoor positioning. In the end, with Java programming language, the positioning system based on optimized fingerprint database is developed in Eclipse environment. Then both original RSSI fingerprint database and the database filtered are tested and experimented in the positioning system, and the result shows that the optimized RSSI fingerprint database can increase the accurate of indoor localization.

Topic: Indoor and Outdoor Localization Technologies
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
GENE EXPRESSION ANALYSIS OF AUTOPHAGY GENES IN POLYCYTHEMIA VERA PATIENTS

Objectives: There is an increasing interest in the correlation between autophagy/mitophagy processes in myeloproliferative neoplasms like polycythemia vera. Especially the Department of Immunology and Medical Genetics at the University of Split, School of Medicine conducts intensive research in this field. The aim of the study was to analyze the expression of autophagy/mitophagy related genes in PV patients in order to find out, if a difference is observable compared to a control group. 
Materials and methods: We used blood samples from eight PV patients to test the expression of seven autophagy/mitophagy related genes via qPCR. We then differentiated into JAK2 positive and negative patients and analyzed accordingly.
Results: After performing qPCR and data analysis, we could see that some genes in JAK2 positive PV patients were expressed more and some less than in the leukemia control. The results were not statistically significant. 
Conclusion: The decreased expression of some autophagy and mitophagy related genes in PV patients could be an indicator for the importance of auto- and mitophagy in PV. Especially Nix and Bnip3, which both showed a decreased expression, might be indicative, because they already have been shown to play a crucial role in cancer and heart disease. Further studies are needed to evaluate the significance of this relationship.

Topic: Myeloproliferative Neoplasms: Diagnosis and Treatment
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Prevalence of Helicobacter pylori infection and atrophic gastritis in patients with dyspeptic symptoms in Myanmar

AIM: To survey the detailed analyses for Helicobacter\ pylori (H. pylori ) infection and gastric mucosal status in Myanmar.<br> METHODS: A total of 252 volunteers with dyspeptic symptoms (155 female and 97 male; mean age of 43.6 ± 14.2 years) was participated in Yangon and Mandalay. The status of H. pylori infection was determined based on 5 different tests including rapid urease test, culture, histology, immunohistochemistry and serology. Histological scores were evaluated according to the update Sydney system and the Operative Link for Gastritis Assessment system. Pepsinogen (PG) Ⅰ and PG Ⅱ were measured using enzyme-linked immunosorbent assays.

Topic: Helicobacter pylori-related gastroenterology studies
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Types of job Match, Overeducation and Labour Mobility

Overeducation is one of the most important mechanisms for labour market adjustment when there is an excess supply of highly skilled workers. However, there is much debate about the consequences of this phenomena and the short- and long-term effects for both the overeducated worker and the economy as a whole. This book contributes to our understanding of recent developments in the research on overeducation by providing a detailed overview of the pertinent theoretical and policy issues.

Topic: Migration, Ethnicity, and Economy
Subfield: Sociology and Political Science
Field: Social Sciences
Domain: Social Sciences
Integral three-dimensional imaging with digital reconstruction

A computed three-dimensional (3-D) display system based on integral imaging is presented. The 3-D image is reconstructed by numerical processing of an optically observed image array formed by a microlens array. The algorithm for reconstructing 3-D images is robust, and it enables us to obtain the images viewed from arbitrary directions. This computer-based image retrieval makes it possible to improve qualities of the image such as contrast, brightness, and resolution by numerical techniques. Also, this method eliminates the need for special purpose optical equipment such as high-quality liquid-crystal display and micro-optics components to display the 3-D images. We present experimental results of 3-D image reconstruction to test and verify the performance of the algorithms and the imaging system.

Topic: Advanced Optical Imaging Technologies
Subfield: Media Technology
Field: Engineering
Domain: Physical Sciences
XVII.—The Geology of Gigha

Gigha is a little-visited island off the western coast of Kintyre and between the latter and the island of Islay. It lies about 5¼ miles south-west of West Loch Tarbert, and a little over 1½ miles from the nearest point of Kintyre where the mainland runs out for 1½ miles in the low, sandy, raised beach of Runahaorine (figs. 1 and 10). The island is 6 miles in length from north-east to south-west, and has a greatest breadth of 2 miles. All round it are innumerable smaller isles, all elongated in the same direction as Gigha. Of these the most important are Cara, 1 mile from north-east to south-west by ½ mile in breadth, and Gigalum, ½ mile by ⅕ mile, and Craro, which is smaller still (figs. 2 and 10). The island forming Ardminish Point, on the eastern coast of Gigha, and Eilean Garbh, at the north-eastern end, will be dealt with here as if they were part of the main island, since at low tide they are connected with it by sandy isthmuses.

Topic: Invertebrate Taxonomy and Ecology
Subfield: Ecology, Evolution, Behavior and Systematics
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Basic Information About Larotrectinib (Vitrakvi)

Larotrectinib is a TRK inhibitor that works against cancers anywhere in the body with NTRK gene fusions. It is the first cancer drug which to be designed from the start to fight a specific genetic mutation, not a traditional cancer type.

Topic: Hermeneutics and Narrative Identity
Subfield: Philosophy
Field: Arts and Humanities
Domain: Social Sciences
Exercise Effects on Health-Related Physical Fitness of Individuals with an Intellectual Disability: A Meta-Analysis

A meta-analysis was conducted to determine the effects of exercise on health-related physical fitness of individuals with an intellectual disability. The data came from 21 individual studies yielding 100 effect sizes (ESs). Large effects were demonstrated for muscular and cardiovascular endurance, moderate for muscular strength, and small for flexibility. No significant effects were found for body composition. Document source and program length influenced muscular and cardiovascular endurance outcomes, as published studies and longer programs produced larger ESs. In addition, program type influenced muscular strength (resistance programs produced larger ESs than combined programs), and program frequency influenced flexibility (higher frequency programs had larger ESs than lower frequency programs). It was concluded that additional research is needed to investigate means to improve body composition, flexibility, and muscular strength. Future studies should upgrade their standards for reporting appropriate statistical information and information related to sample and exercise prescription components.

Topic: Down syndrome and intellectual disability research
Subfield: Public Health, Environmental and Occupational Health
Field: Medicine
Domain: Health Sciences
New York: Roundtable 7, 1992

NYPD patrol services bureau disorder control plan; Police department questionnair fromy the office of the special advisor to the board of police commissioners city of Los Angeles - NYPD; NYPD patrol guide re rapid mobilization, emergency mobilization of off-duty members of service, and use of tear gas; Memo re interview of representatives of NYPD from Richard A Rosenthal and Donald Etra to comparisons study group; Overview of the NYPD by commissioner Lee P Brown, 1992. PART OF SERIES: Materials in the series pertain to the relationship between police officials and the communities they serve in the context of riot prevention and control. Included are interviews and roundtable discussions with law enforcement personnel from several major cities, pamphlets and newsletters produced by the School of Criminal Justice at Michigan State University, several reports and studies pertaining to civil disorder, and a series of articles about community-based policing reproduced by the Traffic Institute. PART OF SUBSERIES: Interviews with police officials from several major jurisdictions about their departments' operations.

Topic: Microbial Natural Products and Biosynthesis
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Hyper-Local Content Is Key—Especially Social Media: A Cross-Country Comparison of Mobile Content in Brazil, China, India, and Nigeria

Until recently, smartphones have been used exclusively in the developed world, but they are now increasingly making inroads in Latin America, Africa, and Asia.Consumers across emerging markets are keen to use mobile apps, but they face significant barriers.For instance, more than one-third of consumers in developing nations find content too expensive, and app stores, which usually are driven by mobile networks or brands such as Google and Apple, require access to credit or banking facilities.Translation presents another major obstacle; many mobile users cannot find the content they are searching for-or any content at all-in their local language.Consumers in emerging markets expect to interact primarily with highly localized mobile social networks and health services, followed by music and entertainment content, and then gaming.This is not to say that news and business content are less important-consumer interaction with all types of content must be taken seriously in order to understand attitudes toward and perceptions of mobile.Upstream's "The Next Mobile Frontier Report"-a survey of mobile consumers in Brazil, China, India, Nigeria (and Vietnam)-ranked content preferences as follows: social networking (82%), music (81%), news (78%), gaming (65%), lifestyle (54%), books (53%), business or financial services (46%), educa-

Topic: Digital Marketing and Social Media
Subfield: Sociology and Political Science
Field: Social Sciences
Domain: Social Sciences
Brain glutathione in vascular mild cognitive impairment and cognitive correlates

Abstract Background Oxidative stress (OS) has been implicated in age‐related neurodegeneration and may be important in prodromal states such as vascular mild cognitive impairment (vMCI). Higher peripheral OS is reported in vMCI patients; however, the role of central antioxidant defenses in vMCI and their correlation to cognition is unclear. Glutathione (GSH) is a major brain antioxidant, and the current study assessed brain GSH in possible vMCI vs. controls. Methods Possible vMCI patients (1 standard deviation (SD) below population norms in verbal memory, executive function (EF), processing speed, or working memory, age 55‐85, and currently enrolled in a 6‐month exercise rehabilitation program due to having 2 or more vascular risk factors or previous vascular event) and cognitively‐normal (CN) controls were recruited. All participants received 1H magnetic resonance spectroscopy (MEscher–GArwood Point Resolved Spectroscopy) to quantify brain GSH at baseline in the anterior cingulate (AC) and occipital lobe (OL). Spectroscopic analysis was completed using the Gannet toolkit (vers. 3.1) in Matlab (vers. 2020b). Results In 43 participants (mVCI n = 22, CN n = 21), AC‐GSH (I.U. ± SD) was higher in mVCI (1.96 ± 0.29) compared to CN (1.64 ± 0.48) (F (1,29.7) = 6.9, p = .01); this difference remained after correcting for cerebrospinal fluid (CSF) volume (F (1,28.4) = 6.5, p = .02), and controlling for age and sex (B [SE] = 0.33 [0.11], p = .007). There was no difference in OL‐GSH before or after correcting for CSF volume. Higher AC‐GSH, but not OL‐GSH, was correlated with poorer global cognition (Montreal Cognitive Assessment ‐Full score, B [SE] = 2.15, p = .03), and poorer EF performance (B [SE] = ‐0.67 [0.25], p &lt; .001). These relationships remained significant after correcting for CSF volume. Conclusion The current study suggests an upregulation of AC glutathione in vMCI, which may reflect a compensatory increase in antioxidants as a response to oxidative stress challenges reported in these patients. Higher brain GSH in the AC region is correlated with poorer global cognition and executive function performance, suggesting a link between local brain antioxidant response and disease‐relevant cognitive domains.

Topic: Sulfur Compounds in Biology
Subfield: Biochemistry
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Polyneuritis and refractory headache overlap with synovitis, acne, pustulosis, hyperostosis and osteitis syndrome

Abstract The primary clinical manifestations of synovitis, acne, pustulosis, hyperostosis and osteitis syndrome are skin lesions and osteitis. We describe a 53‐year‐old woman patient suffering from overlapping refractory headache and polyneuritis (cranial nerves I, II and VII ). She complained of headaches, which recurred every month, followed by bilateral anosmia and left vision loss approximately 1 year later. Prednisolone therapy improved her symptoms; however, her refractory headache, pain in the right shoulder and left hip joint, and rash recurred 1 year after prednisolone withdrawal. Also, the patient presented with left sensorineural deafness and extensive acne on her body, and bone scintigraphy and bone biopsy showed synovitis, acne, pustulosis, hyperostosis and osteitis syndrome. After treatment, her symptoms improved without recurrence. Although synovitis, acne, pustulosis, hyperostosis and osteitis syndrome induced by polyneuritis is rare, it should be considered when clinicians encounter patients presenting with headache and polyneuritis of unknown cause.

Topic: Osteomyelitis and Bone Disorders Research
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Simultaneous detection of two subtypes of extracellular vesicles using ultrabright fluorescent nanosphere-based test strips

In recent years, the cargo profiles of extracellular vesicles (EVs), which were inherited from their parent cells, have emerged as a reliable biomarker for liquid biopsy (LB) in disease diagnosis, prognosis, and treatment monitoring.

Topic: Extracellular vesicles in disease
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
The Commissions Proposal for a Common System of Financial Transaction Tax: A Legal Appraisal

The Commission's proposal for a Directive on a common system of financial transaction tax (FTT) is the first EU proposal for a broadbased FTT. It is proposed to be implemented in the EU by 1 January 2014. The FTT is to be applied to financial transactions with financial instruments where a financial institution is party to the transaction. In this legal appraisal of the proposal, key issues arising in the proposal are discussed, foremost concerning the scope of the tax. The proposal is benchmarked against key design features identified by commentators - the susceptibility to migration to financial transactions not covered by the FTT and to foreign territories. It is concluded that the scope is broad enough with regard to the coverage of financial transactions and that the definition of financial institutions is almost too broad. Migration to other territories will be possible but not as easy to realize as under the abolished Swedish FTT. It is also concluded that there are a number of legal uncertainties in the proposal that need to be clarified.

Topic: Corporate Taxation and Avoidance
Subfield: Accounting
Field: Business, Management and Accounting
Domain: Social Sciences