jimnoneill/paper-to-field-training huggingface dataset:
<i>In Situ</i> Monitoring of Intracellular ATP Variation Based on a Thermoregulated Polymer Nanocomposite

It is necessary to develop reliable chemiluminescence strategies for determination of intracellular adenosine triphosphate (ATP), which is vital in life science and clinical diagnosis. However, the current chemiluminescence methods based on firefly luciferase suffered from low delivery efficiency, unsatisfied targeting performance, and autohydrolysis in living biosystem. To circumvent these drawbacks, a thermoresponsive polymer nanocomposite modified with firefly luciferase and ATP aptamer (PFLNC@aptamer) was fabricated, which targeted ATP and determined the intracellular ATP levels via measuring the chemiluminescence signals at different temperatures. The PFLNC@aptamer exhibited capability for the enzymolysis efficiency regulation, increased 21.0% with temperature change from 37.0 to 25.0 °C. The ATP detection limit was 3.3 nM with a linear relationship from 10.0 nM to 0.1 mM. Moreover, the thermoresponsive nanocomposite could also effectively avoid the interference during delivering firefly luciferase into the living cells and effectively discriminate ATP via the immobilized ATP aptamer, which further confirmed its reliability for practical applications. It paves a specific avenue for effective intracellular ATP monitoring in fundamental and applied research.

Topic: Advanced biosensing and bioanalysis techniques
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
FLEUR-DE-SEINE

Performer: YVES MONTAND; Bob Castella et ses Rythmes Writer: Emile Spencer; Disle et E. Jouillot Accompagnement. Digitized at 78 revolutions per minute. Four stylii were used to transfer this record. They are 3.5mil truncated eliptical, 2.3mil truncated conical, 2.8mil truncated conical, 3.3mil truncated conical. These were recorded flat and then also equalized with Turnover: 300.0, Rolloff: -10.0. The preferred versions suggested by an audio engineer at George Blood, L.P. have been copied to have the more friendly filenames. Matrix number: KI 10995 Catalog number: DO 2035 Other IDs from the record include: KI 10995

Topic: Magnetic confinement fusion research
Subfield: Nuclear and High Energy Physics
Field: Physics and Astronomy
Domain: Physical Sciences
Observations on the structure and the nature of ‘<i>NR</i> bodies’ found in the testes of bed-bug

1. NR bodies show special affinity for neutral red. They are rounded or irregular in outline, measuring 0·25–12·5µ in diameter. 2. In fixed preparations NR bodies appear as spherical and vacuolated structures. 3. RNA is present in abundance in mature NR bodies, but while DNA is present in variable amount, the Feulgen technique shows that in their very young stage NR bodies are rich in DNA. 4. Glycogen is absent from the NR bodies, but Hale's technique provides evidence that the hyaluronic acid type of polysaccharide is present in them. 5. By Gomori's modified technique the presence of alkaline phosphatase in the NR body was demonstrated. 6. The mature NR bodies multiply by a process of budding. Small buds or spherules rich in DNA infect fresh spermatogonial cells, the latter being converted into the ‘pathological cells’ of Hertig.

Topic: Hemiptera Insect Studies
Subfield: Ecology, Evolution, Behavior and Systematics
Field: Agricultural and Biological Sciences
Domain: Life Sciences
THE POSSIBILITY OF EDUCATION ABOUT RELIGIOUS CULTURE IN PUBLIC SCHOOLS

\nIn Japan, religious education is usually divided into three categories; education about religions or religious knowledge, education to inculcate religious sentiment, and sectarian or confessional education. Education about religion can be taught at public schools, while confessional education is prohibited. Long discussions have been held regarding the inculcation of religious sentiment in postwar Japan. Some insist that it should be taught even at public schools, and others oppose this claim mainly based on the reflection of the influence of State Shinto in the prewar period, when the state and religion (Shrine Shinto) were deeply interconnected. The Basic Law on Education was revised in December, 2006, soon after the inauguration of the Abe cabinet. The article concerning religious education was moderated slightly with the words “general learning regarding religion” added to the sentence. However, as Japanese society has tended to avoid discussions on religious education in the postwar period, it might be quite difficult to establish a new education plan based on the former perspectives, especially regarding the inculcation of religious sentiment. The idea of education in religious culture has been introduced to seek for a new perspective on the problem. This perspective aims to promote a deeper understanding of the Japanese people’s own religious culture, as well as that of foreign nations. According to this plan, such religious education could be introduced even at public schools. Surveys and other research data from in recent years indicate that religious culture education would be far more acceptable to people, including students, than education for the “inculcation of religious sentiment.” Moreover, in the age of globalization, this type of religious education seems to be necessary for countries other than Japan as well. As a matter of fact, similar attempts can be observed in the U.S., the U.K., Australia, and other countries. These nations seem to share the following common problems: influence of globalization, influence of the information age (especially the Internet), and the “cults” problem.\n

Topic: Education and Islamic Studies
Subfield: Education
Field: Social Sciences
Domain: Social Sciences
Risk Assessment - Factors Likely to Affect System Implementation

8-9 February 2005 This paper looks at the connection between system failure and a lack of investment in education and training. It reviews the evidence, starting with a Report by the Royal Academy of Engineering and the British Computer Society, The Challenges of Complex IT Project (2004). It then goes on to consider why education and training is such a low priority. The conclusion is that the National Programme for IT will not succeed unless resources are ring fenced for education and training.

Topic: Hermeneutics and Narrative Identity
Subfield: Philosophy
Field: Arts and Humanities
Domain: Social Sciences
Particle-Swarm Optimization in Antenna Design: Optimization of Log-Periodic Dipole Arrays

This paper presents a short tutorial and overview of optimization algorithms based on particle-swarm schemes, and their application to solving electromagnetic problems. As a practical example, a particle-swarm optimization (PSO) tool has been applied in conjunction with the Numerical Electromagnetics Code (NEC) to get the design curves of optimized log-periodic dipole arrays (LPDAs). The graphs show the performance of several radiation parameters (directive gain, front-to-back ratio, bandwidth, SWR, and half-power beamwidth in the E and H planes) as a function of typical design parameters of log-periodic dipole arrays (geometrical parameters and characteristics of the feed). Examples of optimized antennas are given, and their performance is compared to that of standard log-periodic dipole arrays.

Topic: Antenna Design and Optimization
Subfield: Aerospace Engineering
Field: Engineering
Domain: Physical Sciences
Dual-Domain Single Image De-Raining Using Conditional Generative Adversarial Network

This paper presents a novel method for a single image rain streak removal problem which exploits the spatial as well as wavelet transformed coefficients of the rainy images. The proposed method adopts the Conditional Generative Adversarial Network [1] framework and consists of two following networks: Generator and Discriminator. The generator model receives the input from both spatial, frequency domain of the rainy image and yields five de-rained image candidates. A Deep Residual Network [2] has been used to merge these derained candidates and predict a single de-rained image. To ensure the visual quality of the de-rained image, Perceptual loss function [3] in addition to adversarial training has been incorporated. Extensive experiments on the synthetic and realworld rainy images dataset reveal an improvement over the existing state-of-the-art methods [4], [5] by ~ 1.08%, 2.57% in Structural Similarity Index [6] and ~ 7.39%, 9.95% in Peak signal-to-noise ratio respectively.

Topic: Image Enhancement Techniques
Subfield: Computer Vision and Pattern Recognition
Field: Computer Science
Domain: Physical Sciences
Analysis of kinetic models for chalcopyrite flotation: effect of operating parameters

In this research, the influence of pH, particle size, the type of collector and frother and their concentration was studied on the flotation kinetic parameters, rate constant and ultimate recovery. In this regard, three different kinetic flotation models such as Classical model, Klimpel model, and fully mixed reactor model were applied to evaluate the batch flotation results. The results showed that the fully mixed reactor model gave the best fit to the experimental data. In the second stage, variation of flotation rate constants and ultimate recoveries are investigated with respect to particle size and chemical conditions. It was found that the rate constant (k) and ultimate recovery (R∞) were strongly dependent on pH and types of collector and their frother and dosages. It was also observed that best values of R∞, K, separation efficiency, and selectivity index are obtained in the presence of a mixture of isopropyl-n-ethyl thionocarbamate (C4132) and Flomin C7240 (a mixture of 10–20 wt% sodium alkyl dithiophosphate and 20–30 wt% sodium mercaptobenzothiozole) as the collector and a mixture of polypropylene glycol (A65) and methyl isobutyl carbinol as the frother and dosages of 40 g/ton for both reagents, respectively.

Topic: Minerals Flotation and Separation Techniques
Subfield: Water Science and Technology
Field: Environmental Science
Domain: Physical Sciences
Corporate governance and profitability. Value relevance of compliance to corporate governance best practice in Italian listed SMEs

The debate on the relation between corporate governance quality, firm value and performance is on the top since last years. Literature research shows segmented evidence on the topic depending on the market observed, the sample considered, the statistical model adopted and specific characteristics considered. So that, the question is still open. The paper analyses the relation between corporate governance characteristics as noted in Italian code for listed firms and profitability focusing on industrial listed small-medium entities (SMEs) in Italy in the fiscal year 2011. Results identify negative association between 'good' corporate governance and profitability, both for ROS and EBITDA/sales ratio.

Topic: Corporate Finance and Governance
Subfield: Accounting
Field: Business, Management and Accounting
Domain: Social Sciences
Liquid\nMetal-Based Magnetorheological Fluid with a\nLarge Magnetocaloric Effect

Herein,\nliquid metal and Mn&lt;sub&gt;0.6&lt;/sub&gt;Fe&lt;sub&gt;0.4&lt;/sub&gt;NiGe&lt;sub&gt;0.54&lt;/sub&gt;Si&lt;sub&gt;0.46&lt;/sub&gt; particles with a large magnetocaloric\neffect are adopted to prepare a novel magnetocaloric suspension named\nliquid metal-based magnetorheological fluid (LM&lt;sup&gt;2&lt;/sup&gt;RF), which\ncan well solve the problems of brittleness, low thermal conductivity,\nand poor machinability in classical magnetocaloric materials. The\nlow melting point and high boiling point of liquid metal could significantly\nwiden the operating temperature range of such a fluid. As a carrier,\nthe high thermal conductivity, low viscosity, and large density of\nliquid metal display advantageous to heat transfer. The maximum loading\nfraction is 19.5 wt %, while LM&lt;sup&gt;2&lt;/sup&gt;RF features the liquid\nstate. A series of tests are conducted to investigate the alloying\nbehavior in LM&lt;sup&gt;2&lt;/sup&gt;RF. It is found that galinstan will react\nwith Mn&lt;sub&gt;0.6&lt;/sub&gt;Fe&lt;sub&gt;0.4&lt;/sub&gt;NiGe&lt;sub&gt;0.54&lt;/sub&gt;Si&lt;sub&gt;0.46&lt;/sub&gt; particles and form MnGa alloy. However, the reaction rate is very\nslow and the generated MnGa alloy is passivating. Consequently, the\nquantity of MnGa alloy is too sparse to affect the magnetocaloric\nperformance of LM&lt;sup&gt;2&lt;/sup&gt;RF. Overall, the LM&lt;sup&gt;2&lt;/sup&gt;RF exhibits\na large MCE at around room temperature with a lower magnetic hysteresis\nloss, and the transition temperature (&lt;i&gt;T&lt;/i&gt;&lt;sub&gt;m&lt;/sub&gt;) remains constant in 60 days. This work demonstrates the outstanding\nperformance of LM&lt;sup&gt;2&lt;/sup&gt;RF and provides a promising alternative\nto MCE materials for practical magnetic refrigeration.

Topic: Vibration Control and Rheological Fluids
Subfield: Civil and Structural Engineering
Field: Engineering
Domain: Physical Sciences
Tax Evasion and Monopoly Output Decisions with Endogenous Probability of Detection

The profit tax evasion and monopoly output decisions are examined in the uncerAbstract tainty model with endogenous probability of detection. When a rational but amoral, profit-understated firm in advance considers the probability of detection and punishment, the optimal output rate and the optimal cost-overstating factor will be deliberately determined The analysis shows that the effect of profit tax may be either production in excess, or less than, the conventional monopoly output level. The result suggests that the profit tax cannot be relied on for reducing the monopoly distortion.

Topic: Taxation and Compliance Studies
Subfield: Economics and Econometrics
Field: Economics, Econometrics and Finance
Domain: Social Sciences
O2‐03–01: Exploring β‐secretase activity in lipid raft microdomains

β-amyloid (Aβ) peptides are generated from amyloid precursor protein (APP) by the sequential proteolysis mediated by BACE1 and γ-secretase. Previously, biochemical fractionation and antibody co-patching methods revealed that a significant fraction of BACE1 is present in cholesterol-rich membrane microdomains, termed lipid rafts. Disruption of BACE1 association with lipid rafts by cholesterol depletion inhibits or elevates Aβ production depending on the extent of cholesterol depletion. The intricate interplay between cholesterol levels, APP trafficking and BACE1 processing is not clearly understood because cholesterol depletion has pleiotrophic effects on Golgi morphology, vesicular trafficking, and membrane bulk fluidity. We explored the function of BACE1 in raft and non-raft microdomains without altering the cellular cholesterol level. We generated stable cell lines expressing mutant BACE1 lacking the sites of S-palmitoylation, a post-translational modification responsible for targeting a variety of cytosolic and transmembrane proteins to lipid rafts. Fractionation studies showed near complete displacement of this BACE1 mutant from the lipid raft microdomains. Further characterization revealed that the subcellular localization, stability and cell surface arrival of S-palmitoylation-deficient BACE1 was similar to wild type BACE1. Surprisingly, predominant non-raft localization of BACE1 variant did not have any functional influence on BACE1 processing of APP in stably transfected BACE1-/- fibroblast or N2a cell lines. Our results indicate that lipid raft residence of BACE1 is not obligatory for cleavage of APP, and that BACE1 can efficiently cleave APP in both raft and non-raft microdomains.

Topic: Alzheimer's disease research and treatments
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Lactiferous vessel detection from microscopic cross-sectional images

This paper presents the methods to detect and segment lactiferous vessels or rubber latex vessels from gray scale microscopic cross-sectional images using polynomial curve-fitting with maximum and minimum stationary points. Polynomial curve-fitting is used to detect the location of lactiferous vessels from an image of a non-dyed cross-sectional slice which was taken by a digital camera through microscope lens. The lactiferous vessels are then segmented from an image using maximum and minimum stationary points with morphological closing operation. Two species of rubber trees of age between one to two years old are sampled namely, RRIM600 and RRIT251. Two data sets contain 30 microscopic cross-sectional images of one-year old rubber tree's stems from each species are used in the experiments and the results reveal that most of the lactiferous vessel areas can be segmented correctly.

Topic: Remote Sensing and LiDAR Applications
Subfield: Environmental Engineering
Field: Environmental Science
Domain: Physical Sciences
A complication of percutaneous cannulation of the internal jugular vein

Reviews of the use of the technique of percutaneous cannulation of the internal jugular vein for central venous pressure monitoring have indicated that it is free from serious complications. A patient is reported here in whom the ascending cervical artery was damaged during attempted cannulation of the internal jugular vein prior to aortic valve replacement. Haemorrhage from this site after the operation led initially to an extrapleural haematoma and soon afterwards to a haemothorax, which proved fatal despite immediate resuscitation and exploration.

Topic: Central Venous Catheters and Hemodialysis
Subfield: Emergency Medical Services
Field: Health Professions
Domain: Health Sciences
[Doxapram in stable obstructive chronic respiratory insufficiency. A prospective study on 15 cases (author's transl)].

The oral form of Doxapram, a respiratory analeptic drug with central action, was tested prospectively and successively, the subject being his own control, in 15 patients with stable obstructive chronic respiratory insufficiency (OCRI), but not infected and with a high PaCO2. The efficiency of the product was judged notable nine times, medium four times; only twice was its action judged inefficient. Retrospectively, it was not possible to define the group OCRI with high PaCO2, responding or not to the oral form of Doxapram. In these conditions, the utility of prescribing this molecule is subjected to the control of gasometric efficiency before and after absorption. The frequent efficiency of the product leads to the consideration of a regular use in some obstructive chronic respiratory insufficiency.

Topic: Chronic Obstructive Pulmonary Disease (COPD) Research
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Angiosarcoma of penis

A 29-year-old male patient presented with proliferative lesion in the glans penis without any inguinal lymphadenopathy. The biopsy showed a highly vascular malignant lesion. Subsequent metastasis work-up was negative. Partial amputation of the penis with close follow-up was performed. Final histopathology was consistent with angiosarcoma of the penis.

Topic: Vascular Tumors and Angiosarcomas
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Screening of ligninolytic enzymes produced by Ganoderma lucidum in solid residues from the Amazon

White rot fungi have one of the most efficient oxidative lignin degradation systems, and present an enzymatic complex that is responsible for digesting lignocellulosic matter. The aim of this study was to evaluate the production of laccase and total peroxidases by Ganoderma lucidum via solid-state fermentation using açaí seeds and marupá (Simarouba amara) sawdust unsupplemented and supplemented with wheat, corn and rice bran. G. lucidum was inoculated in substrates prepared with the residues and incubated at 25 ºC. Enzymatic extractions were performed on the culture substrates every 2 days for 30 days. The enzymatic activities were determined using ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), with the addition of the enzymatic cofactor H2O2 for total peroxidases. The laccase activity was higher in the supplemented residues, with emphasis on the açaí-based substrate on the 16th day of cultivation, while in marupá the maximum activity was on the 6th day. In the unsupplemented açaí residue, the maximum peak of activity was on the 8th day and, in marupá, no fungal growth was observed. As for total peroxidases, G. lucidum cultivated in the supplemented açaí substrates showed activity peaks on the 8th, 12th, 16th and 28th day, and on 6th and 12th day under unsupplemented conditions. While, in the marupá, total peroxidase activity was observed only on the 6th day of cultivation. Thus, G. lucidum showed potential for producing laccases and total peroxidases, with the substrate supplementation inducing the synthesis of these enzymes.

Topic: Enzyme-mediated dye degradation
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
The Thesis Submitted for the Degree of Master of Science Plant Breeding and Biotechnology

Correction of drought-tolerant wheat varieties is one of the goals of corrective programs. Grain yield in drought stress conditions depends on many phonological, morphological and physiological characteristics. Induction of mutation is an effective way to increase the genetic diversity of plants, especially for traits with low genetic diversity. In this experiment, in order to study the effect of different dose of gamma rays (control, 150, 200 and 250 G) on morphological, physiological and NAC and MYB gene expression in four different cultivars of wheat, Sistan (Afshian wheat, Bolanian coffee E, white whites and camel teeth) under drought stress conditions, an experiment was conducted in a research greenhouse of the Faculty of Agriculture, University of Zabol. Firstly, irradiated
wheat seeds from a Sistan field research center were cultivated in a pot in a factorial arrangement in a completely randomized design with three replications.
After emergence of seedlings to the 4-5 stage, drought stress was applied at 5 levels (5, 10, 15, 20, 25 percent). After harvesting, the traits were morphological traits (stem length, root length, root number, leaf length, leaf width, leaf number, fresh weight of the shoot, aerobic weight of the shoot, root fresh weight, root dry
weight) Antioxidant enzymes (polyphenol oxidase, catalase, guaiacol peroxidase, ascorbate peroxidase), photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoid and total), as well as the pattern of expression of NAC and MYB genes were studied. And the results indicated that different gamma-ray dosages had a significant effect on the promotion of most traits related to drought tolerance in
wheat, so that by increasing the dose of gamma treatments and drought stress, the resistance levels of the samples Compared to control, the highest expression of the
studied genes was observed in 250g radionuclide treatment and drought stress of 5% crop capacity. Also, among the cultivars studied, the camel's genotype showed a better response to drought and irradiation treatments, which was introduced as a resistant cultivar in this experiment.

Topic: Plant Genetic and Mutation Studies
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences