jimnoneill/paper-to-field-training huggingface dataset:
Identification of a Potential Mitotic Function for the Mammalian Nup50

Mitosis is a conserved process in which the genetic material, DNA, is equally segregated between two daughter cells. DNA is contained in the nucleus of the eukaryotic cell and surrounded by the nuclear envelope. Multi protein complexes known as the Nuclear Pore Complexes (NPCs) embed within the nuclear envelope and regulate the transport of molecules in and out of the nucleus. Surprisingly, in Aspergillus nidulans, the model system used in my study, a nuclear pore complex protein Nup2 undergoes a unique translocation to chromatin during mitosis and is essential for proper mitotic progression. Interestingly, the Nup2 homolog in higher eukaryotes, Nup50, undergoes the same translocation. Therefore, the purpose of this study is to test whether Nup50 can translocate onto chromatin in Aspergillus nidulans and complement the mitotic function of Nup2. In order to test this hypothesis, the Nup50 gene was integrated into A. nidulans using homologous recombination. Four way fusion PCR was used to generate a DNA cassette that contains the Nup50 gene fused to EGFP2 marker and its expression under control of the inducible promoter alcA. Once Nup50 was introduced into A. nidulans, Nup2 was deleted in the background. So far, we have discovered that Nup50 is present in the nucleus at interphase and disperses throughout the cell during mitosis in the absence or presence of the Aspergillus nidulans Nup2. Results showed that Nup50 cannot complement Nup2’s function perhaps because it is not located at either the NPCs of mitotic chromatin. Furthermore, mmNup50 is a much smaller protein than Nup2 and it lacks a specific domain which is present in Nup2, termed domain 4. Domain 4 has been shown to be sufficient and necessary for Nup2’s location to the pore and DNA. This suggests that introducing domain 4 into mmNup50 might tether it to the pore at interphase and mitotic chromatin. We plan to create a version of Nup50 that carries domain 4 and replace the endogenous An-Nup2 with this version. We can then ask whether this version of Nup50 can complement Nup2’s function. This study may provide evidence that the novel essential mitotic function of the A. nidulans Nup2 is conserved in humans. Knowledge gained from these experiments might help us identify a potentially novel essential function of the mammalian Nucleoporin 50 in mitosis, which is important to understanding the initiation and progression of human diseases that can arise from defective mitosis such as cancer.

Topic: Hermeneutics and Narrative Identity
Subfield: Philosophy
Field: Arts and Humanities
Domain: Social Sciences
Improving immunity against chronic virus infection using an autoimmunity associated Ptpn22 allele.

Abstract Dysfunctional dendritic cells (DC) lead to inadequate T cell activation causing chronic virus infection. Enhancing both DC and T cell function can prevent chronic virus infection. An approach to enhance immune function is to harness the immune-activating phenotype driven by the autoimmunity-associated allele of PTPN22, which is expressed in all immune cells. We previously found that mice expressing the Ptpn22 autoimmunity-associated allele (Ptpn22-R619W) successfully clear Lymphocytic choriomeningitis virus clone 13 (LCMV-cl13) unlike wildtype (WT) mice. This correlates with a more immunostimulatory DC phenotype and enhanced T cell function. However, the mechanism Ptpn22-R619W enhances DC and T cell function remains unclear. Using our CRISPR/cas9 generated Ptpn22-R619W mice, we addressed the hypothesis that Ptpn22-R619W increases DC immunostimulatory proteins which increases anti-viral T cell activation during virus infection. Post infection, Ptpn22-R619W DCs have reduced infection, increased CD86, and decreased PD-L1 expression both in vivo and ex vivo. Additionally, Ptpn22-R619W DCs have a differential gene expression profile. Also, Ptpn22-R619W mice have enhanced CD8 T cell function against the LCMV-cl13 minor epitope, GP276, but not the major epitope. Lastly, co-culture experiments demonstrate that Ptpn22-R619W in both DC and T cells is necessary to enhance T cell function. These results show how Ptpn22-R619W prevents DC and T cell dysfunction during virus infection.

Topic: Diabetes and associated disorders
Subfield: Genetics
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
An Empirical Study of the Factors of Teleworking and the Moderating Effect of Work Colleague Support

The purpose of the present study was to examine the relationships between motivation factors and attitude towards teleworking and explore the moderating effect of work colleague support on that relationship. For this, the present study collected data from 343 IT professionals in South Korean through a survey method. In the results, first, employees who perceived more advantages accruing from teleworking to themselves have a more favorable attitude towards teleworking. And, employees who perceived more disadvantages accruing from teleworking to themselves will have a less favorable attitude towards teleworking. Second, negative relationship between disadvantages of teleworking and attitude towards teleworking is weaker for participants who get more work colleague support rather than less one. However, work colleague support was found to have no significance on the relationship between advantages of teleworking and attitude towards teleworking.

Topic: Work-Family Balance Challenges
Subfield: Sociology and Political Science
Field: Social Sciences
Domain: Social Sciences
“Forget Me Not”: Free Black Women and Sentimentality: Color Plate 3.

Friendship albums, blank volumes with decorative covers, emerged around 1825 as part of a growing market of women's print culture. Sentimentalism, the chief vocabulary of the friendship album genre, targeted white women consumers and largely ignored black women. However, freeborn African Americans rigorously engaged sentimental literature in the pages of the friendship album, recasting this artifact as a specimen of antebellum black print culture. This essay explores the production of African American friendship albums and examines artistic and literary contributions by freeborn black women writers.

Topic: Literature: history, themes, analysis
Subfield: Literature and Literary Theory
Field: Arts and Humanities
Domain: Social Sciences
Cerebral Autoregulation in Children During Sevoflurane Anaesthesia

Survey of Anesthesiology: February 2004 - Volume 48 - Issue 1 - p 15-16 doi: 10.1097/01.sa.0000108436.59995.aa

Topic: Anesthesia and Neurotoxicity Research
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Whole brain structural covariance with ADOS-SI scores as a result of direct between-group contrasts.

&lt;p&gt;Statistical parametric maps depict brain regions in which gray matter intensity covaried with ADOS Social Impairment (ADOS-SI) score differently between groups. In controls, ADOS-SI scores covaried with frontal regions overlapping with SN including medial frontal wall, anterior cingulate, and frontoinsular cortex. In contrast, in autistic subjects ADOS-SI covaried with posterior brain regions including cuneus, precuneus, parieto-occipital regions, and temporoparietal cortex. scMRI data are T-statistic maps (p&lt;0.05, inclusively masked to the network global map for both groups at p&lt;0.01 FWE) displayed on the average anatomical template of all subjects. scMRI maps from both groups overlaid on a single anatomic volume reveal distinct between-group differences. The left side of the image corresponds to the right side of the brain. SN, salience network.&lt;/p&gt;

Topic: Agricultural Practices and Plant Genetics
Subfield: Plant Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
A theoretical study of the inviscid hypersonic flow about a conical flat-top wing-body combination

16 Slattery, R. E. and Clay, W. G., turbulent wake of hypersonic bodies, ARS Preprint 2673-62 (1962). 17 Lykoudis, P. S., growth of the hypersonic turbulent wake behind blunt and slender bodies, The Rand Corp. Memo. RM-3270-PR (January 1963). 18 Pallone, A. J., Erdos, I. I., Eckerman, J., and McKay, W., Hypersonic laminar wakes and transition studies, AIAA Preprint 63-171 (1963). 19 Lees, L., Hypersonic wakes and trails, ARS Preprint 2662-62(1962). 20 Demetriades, A. and Gold, H., Transition to turbulence in the hypersonic wake of blunt-bluff bodies, ARS J. 32, 14201421 (1962). 21 Demetriades, A. and Gold, H., Correlation of blunt-bluff body wake transition data, Graduate Aeronautical Lab., California Institute of Technology Internal Memo. 12 (September 1962).

Topic: Fluid Dynamics and Turbulent Flows
Subfield: Computational Mechanics
Field: Engineering
Domain: Physical Sciences
Effect of Autoclave Sterilisation on the Dimensional Stability and Tear Strength of Three Silicone Impression Materials

Objectives: There is a risk of spreading infectious material via non-sterile impressions. The aim of this study was to compare the effect of autoclave sterilisation on dimensional stability and tear strength of impression materials. Methods: Twenty-four specimens were produced using a standard ruled test block for each of three impression materials (Affinis, Aquasil and Speedex) to test dimensional stability. Thirty tear strips for each material were prepared for the tear test (ISO 34-1). Specimens were randomly allocated to 3 different groups (autoclaved, disinfected or untreated). A non-contact scanner was used to analyse dimensional change and a universal testing machine was used to determine tear strengths. Results: There were no significant differences in the test dimensions measured for any of the three impression materials following autoclave treatment compared to the disinfected or untreated control groups. The tear strengths were not adversely affected by autoclave sterilisation at 134°C. Conclusion: These addition and condensation-cured silicone impression materials can be steam autoclaved without adverse effects on dimensional accuracy or tear strength.

Topic: Dental materials and restorations
Subfield: Orthodontics
Field: Dentistry
Domain: Health Sciences
Artist unknown :Sketch of Kawiti's pa, Ruapekapeka / compiled from sketches by Capt. Marlow, Lieut Leeds and J P du Moulin. - [London? lithographer unknown, 1846?]

Plan view of Ruapekapeka pa, with elevations around the edge, showing a cross-section of the pa with its tunnels and trenches, and details along the bottom 
Ink annotations


Compare version published in: United service journal, v. 3, Oct 1846, p. 252 (see Photo file print collection)


In: Maori war sketches acquired by Alexander Turnbull


Compare also ink version (with slight differences) in Cartographic Collection at MapColl-832.11hkm/1846/Acc.29011 
Quantity: 1 b&w art print(s). 
Physical Description: Lithograph, b&w, 244 x 279 mm

Topic: Pacific and Southeast Asian Studies
Subfield: Geography, Planning and Development
Field: Social Sciences
Domain: Social Sciences
Beaver herbivory and its effect on cottonwood trees: influence of flooding along matched regulated and unregulated rivers

Abstract We compared beaver ( Castor canadensis ) foraging patterns on Fremont cottonwood ( Populus deltoides subsp. wislizenii ) saplings and the probability of saplings being cut on a 10 km reach of the flow‐regulated Green River and a 8.6 km reach of the free‐flowing Yampa River in northwestern Colorado. We measured the abundance and density of cottonwood on each reach and followed the fates of individually marked saplings in three patches of cottonwood on the Yampa River and two patches on the Green River. Two natural floods on the Yampa River and one controlled flood on the Green River between May 1998 and November 1999 allowed us to assess the effect of flooding on beaver herbivory. Independent of beaver herbivory, flow regulation on the Green River has caused a decrease in number of cottonwood patches per kilometre of river, area of patches per kilometre, and average stem density within cottonwood patches. The number of saplings cut per beaver colony was three times lower on the Green River than on the Yampa River but the probability of a sapling being cut by a beaver was still higher on the Green River because of lower sapling density there. Controlled flooding appeared to increase the rate of foraging on the Green River by inundating patches of cottonwood, which enhanced access by beaver. Our results suggest regulation can magnify the impact of beaver on cottonwood through interrelated effects on plant spatial distribution and cottonwood density, with the result that beaver herbivory will need to be considered in plans to enhance cottonwood populations along regulated rivers. Published in 2002 by John Wiley &amp; Sons, Ltd.

Topic: Ecology and biodiversity studies
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Guanyl Nucleotide Regulation of Human Chorionic Gonadotropin Binding to Rat Luteal Tissue*

Guanyl nucleotides are known to have a dual effect on most hormone-sensitive adenylate cyclase systems, regulating activation of the adenylate cyclase enzyme and binding of hormone to receptor. In the ovary, guanyl nucleotides have been shown to be required for hCG stimulation of luteal adenylate cyclase, but no effect on binding has been observed. Evidence has been obtained which suggests that guanyl nucleotide regulation of hCG binding is masked in most luteal membrane preparations by the presence of a large excess of unregulated receptor. A highly purified urea-washed membrane fraction has been prepared. Binding of hCG to this preparation was decreased (approximately 45%) in the presence of 5'-guanylylimidodiphosphate (GMPPnP). The maximal effect of GMPPnP occurred at 10 microM, with the half-maximal effect at 0.2 microM. These levels compare favorably with the GTP concentrations required for hCG stimulation of luteal adenylate cyclase. Analysis of equilibrium binding experiments showed that GMPPnP acted by reducing the number of binding sites by 45%. However, kinetic experiments suggested that this effect was due to a significant decrease in the affinity of a fraction of the hCG-binding sites. Association of hCG and its receptor was unaffected by the presence of GMPPnP (100 microM), whereas the dissociation of 40-50% of bound hormone was significantly accelerated (30-fold) by its presence. The guanyl nucleotide effect required the presence of MG+2; other divalent cations (Ca+2, Mn+2, and Co+2 could not be substituted. The ratio of beta-adrenergic to hCG-binding sites in the urea-washed heavy membrane preparation was elevated, suggesting that a sizable fraction of uncoupled hCG receptor had been removed. The results show that hCG binding to its luteal receptor is modulated by guanyl nucleotide and suggest that the modulation only occurs in those receptors that are directly coupled to the adenylate cyclase enzyme.

Topic: Coagulation, Bradykinin, Polyphosphates, and Angioedema
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
An investigation of the role of brand image on consumer loyalty

This paper presents an investigation on the role of brand image on customer loyalty on rubber industry. The proposed study designs a questionnaire in Likert scale consists of 27 questions, distributes it among some Iranian experts in rubber industry and analyzes it based on principal component analysis. During the survey, the number questions are reduced to 23. Cronbach alpha is calculated as 0.812 and Kaiser-Meyer-Olkin Measure of Sampling Adequacy and Approx. Chi-Square are 0.671 and 2375, respectively. Based on the results of our survey, we have derived six factors including penetration strategy, infrastructure characteristics, competitive pricing, target marketing strategy, communication strategy and market characteristics.

Topic: Customer Service Quality and Loyalty
Subfield: Organizational Behavior and Human Resource Management
Field: Business, Management and Accounting
Domain: Social Sciences
Studies on plant absorption of fe‐humate

Abstract Humic acid extracted from a Brunizem soil was purified using cationic and anionic exchange resins and EDTA. The purified humic acid was passed through a column of weakly acid cation exchange resin saturated with iron labelled with 59Fe. The Fe‐humate was eluted from the column at pH 2.7. The rate of iron absorption by excised barley roots from solutions of Fe‐humate was studied. Iron absorption by the roots remains steady during the first eight hours. Absorption from solutions of different concentrations showed the presence of a single pattern between 7.14 x 10‐7 M to 2.6 x l0‐4 M iron concentration range. Metabolic inhibitors and low temperature produced only minor effects. Absorption of iron by roots of entire sunflower plants and transport to the shoot was also studied. The results showed that absorption and transport of iron from Fe‐humate was lower than the absorption and transport of iron from FeEDTA or FeCl3. Absorption of iron from Fe‐humate by leaves really occurs but no transport out of the application point was observed.

Topic: Soil Carbon and Nitrogen Dynamics
Subfield: Soil Science
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Screening for Pancreatic Adenocarcinoma Using Signals From Web Search Logs: Feasibility Study and Results

Introduction: People’s online activities can yield clues about their emerging health conditions. We performed an intensive study to explore the feasibility of using anonymized Web query logs to screen for the emergence of pancreatic adenocarcinoma. The methods used statistical analyses of large-scale anonymized search logs considering the symptom queries from millions of people, with the potential application of warning individual searchers about the value of seeking attention from health care professionals. Methods: We identified searchers in logs of online search activity who issued special queries that are suggestive of a recent diagnosis of pancreatic adenocarcinoma. We then went back many months before these landmark queries were made, to examine patterns of symptoms, which were expressed as searches about concerning symptoms. We built statistical classifiers that predicted the future appearance of the landmark queries based on patterns of signals seen in search logs. Results: We found that signals about patterns of queries in search logs can predict the future appearance of queries that are highly suggestive of a diagnosis of pancreatic adenocarcinoma. We showed specifically that we can identify 5% to 15% of cases, while preserving extremely low false-positive rates (0.00001 to 0.0001). Conclusion: Signals in search logs show the possibilities of predicting a forthcoming diagnosis of pancreatic adenocarcinoma from combinations of subtle temporal signals revealed in the queries of searchers.

Topic: Gene expression and cancer classification
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Deep Learning for Screen-Shot Image Demoiréing: A Survey

Image demoir&amp;#x00E9;ing is an important image processing technology in computer vision, used to remove the moir&amp;#x00E9; from images and improve the image quality. In recent years, the image demoir&amp;#x00E9;ing technique based on the deep learning method has gained more attention and achieved good results, but it still has some limitations. This paper aims to provide a review and perspective on the recent advances in deep learning-based image demoir&amp;#x00E9;ing techniques. First, the definition and production principle of the image moir&amp;#x00E9; pattern are given. Common datasets and image quality evalution methods in demoir&amp;#x00E9;ing studies are analyzed. Then two internationally famous competitions in image demoir&amp;#x00E9;ing are introduced. Second, the research status of the supervised demoir&amp;#x00E9;ing technique is summarized from four dimensions: sampling method, model network design, baseline model, and training learning strategy. Recent progress made by the mainstream model of unsupervised deep learning in the field of image demoir&amp;#x00E9;ing is summarized. The typical application of the image demoir&amp;#x00E9;ing technique in panel defect detection and digital radiography is analyzed. The performance and the image quality of the above mentioned models based on different data sets are evaluated in detail. Finally, this paper analyzes and forelocks the problems to be solved in the coming years.

Topic: Industrial Vision Systems and Defect Detection
Subfield: Industrial and Manufacturing Engineering
Field: Engineering
Domain: Physical Sciences
Polyhedral Transformations of Explicitly Parallel Programs

The polyhedral model is a powerful algebraic framework that has enabled significant advances to analyses and transformations of sequential affine (sub)programs, relative to traditional AST-based approaches. However, given the rapid growth of parallel software, there is a need for increased experiences with using polyhedral frameworks for analysis and transformations of explicitly parallel programs. An interesting side effect of supporting explicitly parallel programs is that doing so can also enable analysis and transformation of programs with unanalyzable data accesses within a polyhedral framework, since explicit parallelism can often mitigate the imprecision that accompanies unanalyzable data accesses arising from a variety of sources, including unrestricted pointer aliasing, unknown function calls, and certain classes of non-affine constructs. In this paper, we address the problem of extending polyhedral frameworks to enable analysis and transformation of programs that contain both explicit parallelism and unanalyzable data accesses. A summary of our approach is as follows. As in past work, we first enable conservative dependence analysis of a given region of code; for simplicity, we use an approach based on dummy variables that can work with any polyhedral tool that supports access functions. After obtaining conservative dependences, we use the Fourier-Motzkin elimination method to remove all dummy variables. Next, we identify happens-before relations from the explicitly parallel constructs, and subtract their complement from the conservative dependences. The resulting set of dependences can then be passed on to a polyhedral transformation tool, such as PLuTo, to enable transformation of explicitly-parallel programs with unanalyzable data accesses. To motivate our approach, we studied 18 explicitly-parallel OpenMP benchmarks from the Rodinia benchmark suite, and found that these benchmarks use six classes of non-affine constructs that are commonly found in parallel scientific applications:1) Non-affine subscript expressions, 2) Indirect array subscripts, 3) Use of structs, 4) Calls to user-defined functions, 5) Non-affine loop bounds, and 6) Non-affineif

Topic: Parallel Computing and Optimization Techniques
Subfield: Hardware and Architecture
Field: Computer Science
Domain: Physical Sciences
Notes o N the checklist of e phydridae (Diptera) of Slovakia

New records and other interesting findings of shore flies (Ephydri - dae) from Slovakia are presented here. Two species (dorsata Sten- hammar, 1844 and Ochthera manicata (Fabricius, 1794)) were reported for a first time from Slovakia and another seven species ( Dichaeta caudata (Fallen, 1813), Hydrellia albilabris (Meigen, 1830), Limnellia quadrata (Fallen, 1813), Pa- racoenia fumosa (Stenhammar, 1844), Parydra (Parydra) aquila (Fallen, 1813), Pelina aenea (Fallen, 1813), and Pelina nitens Loew, 1873) were confirmed from Slovakia by complete and reliable records.

Topic: Diptera species taxonomy and behavior
Subfield: Ecology, Evolution, Behavior and Systematics
Field: Agricultural and Biological Sciences
Domain: Life Sciences