jimnoneill/paper-to-field-training huggingface dataset:
Influence of lipoperoxidation on the generation of mitochondrial membrane potential

Mitochondria are the main site of ATP synthesis, which requires the buildup of a membrane potential (ΔΨ). Reactive oxygen species (ROS) generated during electron transport can damage electron transport chain (ETC) components. Unsaturated lipids from mitochondrial membranes are the main targets of ROS attack, leading to ETC dysfunction due to membrane disruption. The hypothesis of this work is that lipoperoxidation resistance may also confer resistance to other mitochondrial parameters (i.e. ΔΨ generation) under oxidative stress conditions. We used the yeast S. cerevisiae as model due to the intrinsic resistance of yeast to lipoperoxidation. Also, the sensitivity of yeast to lipoperoxidation can be enhanced through incorporation of linolenic acid (C18:3) to membranes. Our results shown that in −C18:3 cells, the cytoplasmic and mitochondrial ΔΨ was observed even at concentrations of Fe 2+ up to 50 μM, while in +C18:3 cells, lower Fe 2+ concentrations inhibited ΔΨ buildup. As comparison, ΔΨ from rat liver mitochondria, which are prone to lipoperoxidation, was also inhibited by Fe 2+ treatment. These results suggest that membranes resistant to lipoperoxidation preserve its structural integrity even under strong oxidative conditions and allow a better function of mitochondrial ETC than the observed in lipoperoxidation‐prone mitochondria.

Topic: Mitochondrial Function and Pathology
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
[Construction and identification of lentiviral vector mediated GATA-3 gene RNA interference in mouse].

<b>Objective:</b>The aim of this study was to construct and identify a lentiviral vector for RNA interference of mouse GATA-3.<b>Method:</b>The coding region of the GATA-3 gene sequences were analyzed for a single-stranded oligo design and synthesis.PCR was performed for splicing reaction of synthetic oligo,which was synthesized into the lentiviral vector and transformed into the competent cell DH5α.An expression vector shRNA-GATA-3 was constructed after sequencing and identifying the gene sequence of the recombinant clones.Accoding to the target gene sequence ,four pairs are as following: siRNA-685 group(LV3-GATA-3-Mus-685),siRNA-1152 group (LV3-GATA-3-Mus-1152),siRNA-1615 group(LV3-GATA-3-Mus-1615) and control group ,which were designed and syntheized and builded into the lentiviral carrier.The shRNA vectors were cotransfected with expression vectors into the 293T cell line,and the optimal interference sequence was screened by QPCR. The 293T cells were cotranfected with the constructed lentiviral interference vectors and packing plasmids,the viruses were packed,the virus stock was collected and concentrated by ultracentrifugation,and the titer of the viruses was determined. <b>Result:</b>Gene sequence proved that the recombinant clone gene sequence was correct,and the lentiviral vector was successfully constructed. The relative expression of GATA-3 mRNA in siRNA-1615 group(0.004) was significantly reduced after RNA interference comparing with that in the control group(0.022),siRNA-1152 group(0.009) and siRNA-685 group(0.009). The screened LV3-GATA-3-Mus-1615 ,as the most effective interference sequence,was constructed into the lentiviral vector.The titer of the concentrated virus of LV3-GATA-3-Mus-1615 was 5×108 TU/ml in the viral supernate of 293T cells collected at 48 hours after co-transfection. <b>Conclusion:</b>Alentiviral RNAi expression vector targeting the GATA-3 gene (LV3-GATA-3-Mus-1615) was successfully constrcted and identified.

Topic: Virus-based gene therapy research
Subfield: Genetics
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
M-MSET: Baseline modeling of A320 Heat Exchanger based on modified MSET method

Abstract The aircraft air conditioning system is an important onboard system that affects the safety of aviation operations. The heat exchange performance of the air conditioning system can significantly deteriorate as flight time accumulates. To detect system performance degradation in advance, a baseline modeling method for air conditioning heat exchangers based on modified multivariate state estimation technique (M-MSET) is proposed in this paper. The density peak clustering (DPC) algorithm is used to select the working state of training samples and construct a memory matrix. The adaptive diagonal loading technique is applied to the multivariate state estimation process to reduce the abnormal fluctuations caused by the pathological state of the memory matrix. Finally, the dynamic 3-sigma method is used to detect deviation values. Using real flight data from airlines for performance monitoring and analysis, the experimental results show that the proposed method can effectively detect potential performance degradation of heat exchangers during normal system operation.

Topic: Fault Detection and Control Systems
Subfield: Control and Systems Engineering
Field: Engineering
Domain: Physical Sciences
Changes in the Clinical Competence of Beginner Nurses in Terms of Leadership Competencies of Theirs Head Nurses in Teaching Hospitals of Mashhad University of Medical Sciences

Changes in the Clinical Competence of Beginner Nurses in Terms of Leadership Competencies of Theirs Head Nurses in Teaching Hospitals of Mashhad University of Medical Sciences

Topic: Health and Well-being Studies
Subfield: Clinical Psychology
Field: Psychology
Domain: Social Sciences
Associations of Older Taiwanese Adults’ Personal Attributes and Perceptions of the Neighborhood Environment Concerning Walking for Recreation and Transportation

This study examines the cross-sectional associations between personal and perceived neighborhood environment attributes regarding walking for recreation and transportation among older Taiwanese adults. Data related to personal factors, perceived environmental factors, and time spent engaging in transportation-related and recreational walking were obtained from 1032 older adults aged 65 years and above. The data were analyzed by carrying out an adjusted binary logistic regression. After adjusting for potential confounders, two commonly perceived environmental factors, the presence of sidewalks (PS) and the presence of a destination (PD), were positively associated with 150 min of walking for recreation. Different personal and perceived environmental factors were associated with walking for recreation and transportation. These findings suggest that policy-makers and physical activity intervention designers should develop both common and individual environmental strategies in order to improve and increase awareness of the neighborhood environment to promote recreational and transportation walking behaviors among older adults.

Topic: Urban Transport and Accessibility
Subfield: Transportation
Field: Social Sciences
Domain: Social Sciences
Separation of functionally distinct regions of a macromolecular substrate. Stimulation of tRNA nucleotidyltransferase by a nonreacting fragment of tRNA.

Rabbit liver tRNA nucleotidyltransferase catalyzes the incorporation of AMP and CMP into the model acceptor substrate, cytidine. The apparent Km for cytidine in this reaction is about 80 to 90 mM which is more than 10(4) greater than the Km values for the natural substrates, tRNA lacking the terminal AMP (tRNA-C-C) and tRNA lacking the terminal pCpA (tRNA-C). The Vmax values for the model reaction are only 5% and 2% of those for the reaction with the natural tRNA substrates. Addition of the tRNA fragments, tRNA lacking the terminal XpCpCpA sequence (tRNA-(X - 1)p) and tRNA lacking the terminal CpCpA (tRNA-Xp), greatly stimulates the rate of nucleotide incorporation into cytidine. In the case of CMP incorporation into cytidine, tRNA-Xp stimulates the reaction about 60-fold, to a rate similar to that of the normal reaction with tRNA-C. The tRNA fragment has no effect on the apparent Km of either cytidine or CTP, but only alters the Vmax of the reaction. Stimulation of the model reactions is maximal with tRNA fragments of specific chain lengths. These results provide direct evidence that the nonreacting regions of a substrate molecule play an important role in the catalytic efficiency of an enzyme.

Topic: RNA modifications and cancer
Subfield: Molecular Biology
Field: Biochemistry, Genetics and Molecular Biology
Domain: Life Sciences
Lamb wave based active damage identification in adhesively bonded composite lap joints

Bonding composite structures using adhesives offers several advantages over mechanical fastening such as better flow stress, weight saving, improved fatigue resistance and the ability to join dissimilar structures. The hesitation to adopt adhesively bonded composite joints stems from the lack of knowledge regarding damage initiation and propagation mechanisms within the joint. A means of overcoming this hesitation is to continuously monitor damage in the joint. This study proposes a methodology to conduct structural health monitoring (SHM) of an adhesively bonded composite lap joint using acoustic, guided Lamb waves by detecting, locating and predicting the size of damage. Finite element modeling of a joint in both 2D and 3D is used to test the feasibility of the proposed damage triangulation technique. Experimental validation of the methodology is conducted by detecting the presence, location and size of inflicted damage with the use of tuned guided Lamb waves.

Topic: Ultrasonics and Acoustic Wave Propagation
Subfield: Mechanics of Materials
Field: Engineering
Domain: Physical Sciences
Step by step illustrative video of laparoscopic artificial urinary sphincter implantation in a woman with recurrent stress urinary uncontinence

Submitted by Helena Donato (bibliotecacuf@jmellosaude.pt) on 2019-05-06T20:36:30Z\nNo. of bitstreams: 1\nCEJU.pdf: 351955 bytes, checksum: 62352d35d596da8fadcd261411031367 (MD5)

Topic: Pelvic floor disorders treatments
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Heterogenella dolini Berest 1989

Heterogenella dolini Berest, 1989 —new junior synonym of Heterogenella cambrica (Edwards, 1938) Berest’s (1989) description of H. dolini fails to present characters that would distinguish it from H. cambrica — the reason here to treat the two species as identical. Why Berest (1989) did not even reflect upon the similarity of H. dolini and H. cambrica becomes obvious from a later publication, in which she designated Edwards’s (1938a) Bryomyia cambrica as the type species of a discrete genus, Cervuatina (Berest 1993). The concept of Cervuatina rests upon the ejaculatory apodeme of the holotype specimen, which according to Berest is “well sclerotized, distally biramous, with the branches being dendritic” and thus “strongly different from analogous structures in other genera of the tribe [Bryomyiini]” (Berest 1993). As described earlier (Jaschhof 1998), I interpret the very same specimen differently. A biramous ejaculatory apodeme is not only found in B. cambrica but in all Bryomyiini, where it is rated as a synapomorphy. In the specimen in question, the loop-like posterior extensions of the apodeme are unusually sharply contoured (Jaschhof 1998: fig. 85a), probably as a result of Edwards’s use of a contrast medium, which gives the “dendritic” impression described by Berest (1993). In other words, the generic concept of Cervuatina is based on an artificial structure misinterpreted as an evolutionary novelty (see Jaschhof 1998).

Topic: Diptera species taxonomy and behavior
Subfield: Ecology, Evolution, Behavior and Systematics
Field: Agricultural and Biological Sciences
Domain: Life Sciences
National Dam Safety Program. Feeder Dam at Glens Falls (Inventory Number NY-143), Upper Hudson River Basin, Warren-Saratoga County, New York. Phase 1 inspection Report

Abstract : The Feeder Dam at Glens Falls is a concrete gravity dam having a 615 foot long ungated river spillway section with flashboards including a 20 foot wide inclined logway. Examination of available documents and a visual inspection of the dam did not reveal conditions which constitute an immediate hazard to human life or property. However, significant deficiencies and deterioration was observed at one portion of the dam, the North Bulkhead, so as to warrant additional study and analysis as well as corrective action by maintenance forces. The spillway capacity of the dam, although not having sufficient discharge capacity for passing one-half the Probable Maximum Flood (PMF), is considered to be inadequate. Other deficiencies found during the visual inspection concerned concrete surface deterioration and cracking, non-operable or non-existent gate machinery, and an overall need for increased maintenance of the dam.

Topic: Dam Engineering and Safety
Subfield: Civil and Structural Engineering
Field: Engineering
Domain: Physical Sciences
Preliminary trials against<i>Biomphalaria glabrata</i>of a new molluscicide formulation: gelatin granules containing Bayluscide wettable powder

Tests were conducted with gelatin granules containing Bayluscide (niclosamide) w.p., 23-3% w/w a.i. Granules rendered a simulated field habitat (banana drain) lethal to Biomphalaria glabrata for 40 days when applied at a rate calculated to give 5 mg/1 in the water. At this dose, Bayluscide e.c. was effective for 8-12 days. Granules exposed to various combinations of natural or simulated tropical climatic factors for up to 56 days gave variable kills of snails, apparently unrelated to the factors tested. The rate at which the molluscicide was released from the granules was affected by climatic factors: high temperatures and low humidities slowed the rate, probably by hardening the granules; rainfall and high humidities accelerated the rate, probably by softening or disintegrating the granules. There was no apparent loss of molluscicidal activity in granules exposed for 56 days to various combinations of climatic factors. The results suggest that this formulation may be applied during droughts to temporary habitats when these are dry and accessible. The molluscicide would then be released at the unpredictable start of the next wet season and kill any snails which survive the drought by aestivation.

Topic: Mosquito-borne diseases and control
Subfield: Public Health, Environmental and Occupational Health
Field: Medicine
Domain: Health Sciences
Evolution of Xerox's network systems architecture

Research and development at Xerox's Palo Alto Research Center (PARC) and Office Systems Division (OSD) have led to many advances in an evolving network architecture. As early experimenters in local area networking, researchers at PARC built a prototype of what is becoming the basis for an international standard. Their early work with networked personal workstations, distributed networked services, and local network interconnection provided valuable insight into requirements for higher level protocols. As designers of products such as the Star professional workstation and the Network Systems services, developers at OSD expanded the original PARC network architecture to include features required for the commercial marketplace.

Topic: Mobile Agent-Based Network Management
Subfield: Computer Networks and Communications
Field: Computer Science
Domain: Physical Sciences
Benzimidazole chemistry. II. Alkyl migration of N-alkyl-4-trifluoromethyl-2,6-dinitroanilines on reduction with tin

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBenzimidazole chemistry. II. Alkyl migration of N-alkyl-4-trifluoromethyl-2,6-dinitroanilines on reduction with tinRobert E. Lyle and John L. LaMattinaCite this: J. Org. Chem. 1975, 40, 12, 1863–1864Publication Date (Print):June 1, 1975Publication History Published online1 May 2002Published inissue 1 June 1975https://pubs.acs.org/doi/10.1021/jo00900a050https://doi.org/10.1021/jo00900a050research-articleACS PublicationsRequest reuse permissionsArticle Views97Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

Topic: Synthesis and Characterization of Heterocyclic Compounds
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Relationship Of Parent Communication With Temper Tantrum In Preschool Children (Ages 3-6 Years) In Paud Buah Hati Mother And State 01 Sitiung Year 2020

Tantrum temper is an uncontrolled outburst of emotions or anger caused by an unpleasant atmosphere the child feels, is a disorder of behavior in preschoolers, which is part of the emotional development process of the child. Factors causing temper tantrums are physiological, psychological, parental (communication and parenting) and environmental factors. Effective communication is one of the appropriate handling measures in reducing the incidence of temper tantrums in preschool children. The purpose of this study was to determine the relationship of parental communication with temper tantrums in preschool children (ages 3-6 years) in Paud Buah Hati Bunda and Negeri 01 Sitiung in 2020.&#x0D; The research method used Observational research is Analytical Inferential Hypothesis. The sampling technique used is total sampling with a population of 92 respondents and a total sample of 50 respondents. Analysis of the data used chi square test using SPSS.&#x0D; The results showed that out of 38 people (76%) of respondents the communication between parents was quite large, 34 people (68%) were at risk of temper tantrums and a small portion of 4 people (8%) were not at risk of temper tantrums. Based on statistical test results obtained, p value = 0.000 = 0.05. So that it can be concluded that there is a significant relationship between parental communication with temper tantrums in preschool children (ages 3-6 years) in Paud Buah Hati Bunda and Negeri 01 Sitiung in 2020. It is hoped that the results of this study will be able to communicate well using effective communication and knowing how to deal with temper tantrums

Topic: Child Development and Education
Subfield: Education
Field: Social Sciences
Domain: Social Sciences
A Review of the Carpathian Ephialtini Parasitoids (Hymenoptera, Ichneumonidae, Pimplinae) Associated with Spiders

Abstract A list of the Carpathian species of the tribe Ephialtini associated with spider hosts is provided. Totally, 33 species are present in the region, 27 of them in the Ukrainian part and 22 in Romania. Acrodactyla quadrisculpta (Gravenhorst, 1820), Clistopyga canadensis Provancher, 1880, Schizopyga frigida Cresson, 1870, the genus Sinarachna Townes, 1960, with two species, S. nigricornis (Holmgren, 1860) and S. pallipes (Holmgren, 1860), Tromatobia lineatoria (Villers, 1789), and Zatypota discolor (Holmgren, 1860) are recorded for Ukraine for the fi rst time. Clistopyga sziladyi Kiss, 1959 and Polysphincta vexator Fitton, Shaw &amp; Gauld, 1988 are new Romanian records. Polysphincta longa Kasparyan, 1976 is a new record for Ukraine and Romania. A redescription of Schizopyga coxator Constantineanu, 1973 and illustrated comparative notes about S. coxator and S. circulator (Panzer, 1800) are provided. Th e paper also contains an illustrated key to European species of the genus Clistopyga Gravenhorst, 1829.

Topic: Hymenoptera taxonomy and phylogeny
Subfield: Ecology, Evolution, Behavior and Systematics
Field: Agricultural and Biological Sciences
Domain: Life Sciences
Cracking the coven: Shakespeare, the supernatural, and the female power base

There has been extensive writing and research into the fairy magic and witchcraft practices of the Early Modern Period in the 400-plus years between when Shakespeare's plays were performed and now--even including a tome on demonology by King James I himself. However, as witchcraft and fairy magic are distinctively female realms, with women making up 90% of accused witches and fairy magic being mainly related to domestic duties, one cannot accurately discuss these phenomena in the plays without addressing how they affect the female characters. This project examines the role of the supernatural in three of Shakespeare's plays, A Midsummer Night's Dream, Macbeth, and The Winter's Tale, and how powerful groups of women are affected. By examining how Shakespeare uses these female-based supernatural powers in his plays, one can gain a greater understanding of how the women fit into the drama and, to a larger extent, how they were expected to fit into society. From the examination of the supernatural in these three plays, it can be concluded that Shakespeare uses fairy magic as a means to support patriarchy and keep women in their proper place within the realm of society. Witchcraft, by contrast, is a female-based power which undermines established patriarchal norms and must be destroyed to keep women from becoming too powerful.

Topic: Marriage and Sexual Relationships
Subfield: Sociology and Political Science
Field: Social Sciences
Domain: Social Sciences
Optimized Non-Primary Channel Access Design in IEEE 802.11bn

The IEEE 802.11 standards, culminating in IEEE 802.11be (Wi-Fi 7), have significantly expanded bandwidth capacities from 20 MHz to 320 MHz, marking a crucial evolution in wireless access technology. Despite these advancements, the full potential of these capacities remains largely untapped due to inefficiencies in channel management, in particular, the underutilization of secondary (non-primary) channels when the primary channel is occupied. This paper delves into the Non-Primary Channel Access (NPCA) protocol, initially proposed by the IEEE 802.11 Ultra-High Reliability (UHR) group, aimed at addressing these inefficiencies. Our research not only proposes an analytical model to assess the throughput of NPCA in terms of average throughput but also crucially identifies that the overhead associated with the NPCA protocol is significant and cannot be ignored. This overhead often undermines the effectiveness of the NPCA, challenging the assumption that it is invariably superior to traditional models. Based on these findings, we have developed and simulated a new hybrid model that dynamically integrates the strengths of both legacy and NPCA models. This model overall outperforms the existing models under all channel occupancy conditions, offering a robust solution to enhance throughput efficiency.

Topic: Wireless Networks and Protocols
Subfield: Computer Networks and Communications
Field: Computer Science
Domain: Physical Sciences