Hepatoprotective Activities of Colocasia esculenta (L.) Schottin Mice Model with Liver Injury Induced by Paracetamol

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

This paper uses the model of experimental mouse liver damage with paracetamol to evaluate the liver protective effects of the methanol extract (Colocasia esculenta (L.) Schott) (MCE). After 8 days, the hepatoprotective activities of MCE in mice (damaged by paracetamol) at doses of 500, 1,000 and 2,000 mg/kg/day proved effective as the AST enzyme content decreased by 6.98, 22.84 and 26.59%, respectively; and ALT decreased by 53.17, 56.46 and 57.93%, respectively. MCE at a dose of 1,000 mg/kg/day had a protective effect equivalent to that of silymarin (used in liver treatment) at a dose of 50 mg/kg/day and MCE at a dose of 2,000 mg/kg/day had better effect. Observation of the microscopic liver tissue cross section also revealed that the mice treated with MCE of C. esculenta at doses of 1,000 and 2,000 mg/kg/day showed significantly improvement in their liver tissues compared to the non-treated control group. Keywords: Colocasia esculenta, hepatoprotective, paracetamol. References [1] S. Shahani. Evaluation of hepatoprotective efficacy of APCL-A poly herbal formulation in vivo in rats, Indian Drugs. 36(1999) 628-631.[2] Y. Cui, X. Yang, X. Lu, J. Chen, Y. Zhao. Protective effects of polyphenols-enriched extract from Huangshan Maofeng green tea against CCl4-induced liver injury in mice, Chemico- Biological Interactions 220 (5) (2014) 75-83. https://doi.org/10.1016/j.cbi.2014.06.018 [3] K.C. Choi, W.T. Chung, J.K. Kwon, J.Y. Yu, Y.S. Jang, S.M. Park, S.Y. Lee, J.C. Lee. Inhibitory effects of quercetin on aflatoxin B1 induced hepatic damage in mice, Food Chemical Toxicology, 48 (10) (2010) 2747-2753 . https://doi.org/10.1016/j.fct.2010.07.001[4] E.S. Sabina, J. Samue, S.R. Ramya, S. Patel, N. Mandal, P. Preety, P.P. Mishra, M.K. Rasool. Hepatoprotective and antioxidant potential of Spirulina fusiformis on acetaminophen induced hepatotoxicity in mice, International Journal of Integrative Biology 6 (1) (2009) 1-5. http://ijib.classicrus.com/.../1501.pdf[5] Pham Hoang Ho. Vietnamese plants, episode III. Young Publishing House, (1999), pp. 353 (in Vietnamese).[6] C.O. Eleazu, M. Iroaganachi, K.C. Eleazu. Ameliorative potentials of cocoyam (Colocasia esculenta L.) and unripe plantain (Musa paradisiaca L.) on the relative tissue weights ofstreptozotocin-induced diabetic rats. Journal of Diabetes Research, 197 (2013), https://doi.org/10.1155/2013/160964.[7] R.C. Cambie, L.R. Ferguson. Potential functional foods in the traditional Maori diet. Mutation Research Letters 523–524 (2003) 109-117. https://doi.org/10.1016/s0027-5107(02)00344-5[8] C.L. Abraham, K. Yoshinori, T. Masakuni, I. Hironori, O. Hirosuke, T. Hajime. Flavonoid glycosides in the shoot system of Okinawa Taumu (Colocasia esculenta S). Food Chemistry 119 (2010) 630- 635. https://doi.org/10.1016/j.foodchem.2009.07.004[9] P.J. Bouic, D. Etsebeth, R.W. Liebenberg, C.F. Albrecht, K. Pegel, J.P.P. Van. Beta sitosterol and beta sitosterol glycoside stimulate human peripheral blood lymphocyte proliferation: Implications for their use as an immunomodulatory vitamin combination. International Journal of Immuno-pharmacology 18 (12) (1996) 693-700. https://doi.org/10.1016/S0192-0561(97)85551-8.[10] Vo Van Chi. Dictionary of Vietnamese medicinal plants. Medical Publishing House (1997), pp. 871-873 (in Vietnamese).[11] Do Trung Dam. Method of determining drug toxicity. Medical Publishing House, Hanoi (2014), pp. 11-137 (in Vietnamese). [12] J.T. Litchfield, F. Wilcoxon. A simplified method of evaluating dose-effect experiments, Journal of Pharmcology and Experimental Therapeutics, 96 (2) (1949) 99-113. PMID: 18152921 [13] Doan Thi Nhu, Do Trung Dam, Pham Duy Mai. Method research of the pharmacological effect of drugs from medicinal herbs, Science and Technics Publishing House, Hanoi (2006), pp. 142 (in Vietnamese).[14] A. Godwin. Histochemical uses of haematoxylin - A Review. JPCS 1 (2011) 24-34. [15] Ho Thi Thanh Huyen, Thai Nguyen Hung Thu, Nguyen Thai An. Some results of plan microscopic studies and chemical composition of Bombax malabaricum DC., Bombacaceae, Proceeding Pharma Indochina VII, 10/2011, The 7th Indochina Conference on Pharmaceutical science, Bangkok, Thai Lan (2011) 270-274.[16] A.N. Chinonyelum, A.P. Uwadiegwu, O.C. Nwachukwu, O. Emmanuel. Evaluation of hepatoprotective activity of Colocasia esculenta (L. Schott) leaves on thioacetamide-induced hepatotoxicity in rats. Pakistan Journal Pharmaceutical Sciences, 28 (6S) (2015) 2237-2241. [17] O.F. Dayo, B.C. Eluke, E.O. Ukaejiofo, O.L. Olayinka, C.I. Johnpaul, O.A.K .Tajudeen, I. Chinwe, L.S. Adetona. Hepatoproactive and haematopoietic modulatory efficacy of leaf extract of Colocasia esculenta in Albino wistar rats. International Journal of Tropical Medicine 12 (3-6) (2017) 35-41. https://doi.org/3923/ijtmed.2017.35.41.