Influence of bcl-2 Small Interference RNA on the Sensibility of Endometrial Carcinoma RL-952 Cells to Doxorubicin

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Objective To research the influence of bcl- 2 targeting siRNA on the sensibility of endometrial carcinoma RL- 952 cells to doxorubicin( DOX). Methods From May to August in 2014,siRNA interference sequence were transfected into endometrial carcinoma RL- 952 cells. The RL- 952 cells were divided into three trial groups: blank control group,bcl- 2 siRNA interference group( with transfected bcl- 2 siRNA interference sequence) and negative control group( with transfected siRNA empty sequence). The transfection efficiency of bcl- 2 siRNA interference group and the protein expression level of bcl- 2 of the three groups were tested. The RL- 952 cells were divided into five groups: control blank group,negative control group( with transfected siRNA empty sequence), interference group( with transfected bcl- 2 siRNA interference sequence),DOC group( added with 5 μg / ml DOX),and combination group( with transfected siRNA interference sequence and5 μg / ml DOX). Cell viability,cell apoptosis rate,the activity of Caspase- 3 and Caspase- 9 and cytochrome C level of the five groups were tested. Results The efficiency of transfection from siRNA interference sequence to endometrial carcinoma RL- 952 cells was( 94. 6 ± 12. 5) %. The three trial groups were significantly different in the protein expression level of bcl- 2( F = 8. 75,P 0. 05). The bcl- 2 siRNA interference group was lower than blank control group and negative control group in the protein expression level( q = 3. 99,2. 87; P 0. 01). The five groups were significantly different in cell viability( F= 42. 52,P 0. 05). Interference group, DOC group and combination group were lower than blank control group in cell viability( q = 7. 66,8. 64,6. 89; P 0. 05); combination group was lower than DOX group in cell viability( q = 6. 76,P 0. 01). The five groups were significantly different in cell apoptosis rate( F = 112. 34,P 0. 05). Interference group,DOX group and combination group were higher than blank control group in cell apoptosis rate( q = 5. 78,4. 99,6. 21; P 0. 05);combination group was higher than DOX group in cell apoptosis rate( q = 4. 89,P 0. 01). The five groups were significantly different in the activity of Caspase- 3 and Caspase- 9 and cytochrome C level( P 0. 05). Interference group,DOX group and combination group were higher than the blank control group in the activity of Caspase- 3 and Caspase- 9 and cytochrome C level( P 0. 05); DOX group,and combination group were higher than negative control group in the activity of Caspase- 3( P 0. 05),and interference group,DOX group and combination group were higher than negative control gorup in the activity of Caspase- 9( P 0. 05); combination group was higher than interference group in the activity of Caspase- 3( P 0. 05),and DOX group and combination group were higher than interference group in the activity of Caspase- 9( P 0. 05); combination group was higher than DOX group in the activity of Caspase- 3 and Caspase- 9 and cytochrome C level( P 0. 05). Conclusion bcl- 2 targeting siRNA interference sequence can increase the sensitivity of endometrial carcinoma RL- 952 cells to DOX.bcl- 2 gene may be a potential target in the gene therapy of endometrial carcinoma.