jimnoneill/paper-to-field-training huggingface dataset:
Transcriptomic and Reverse Genetic Analyses of Aedes aegypti Cultured Cells and Midgut Epithelial Cells upon Heme Exposure

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

The female mosquito Aedes aegypti requires amino acids and other nutrients like heme and iron from a blood meal to initiate vitellogenesis. Heme is a pro-oxidant molecule that acts as a nutrient, signaling molecule and in large quantities, as a toxin. Ae. aegypti has developed a few strategies to handle heme toxicity, as during a typical meal ~10mM is released into the midgut lumen. These strategies include heme aggregation to the peritrophic matrix and the degradation of heme by heme oxygenase in the cytosol of the midgut epithelium. However, despite the importance of heme as a nutrient and toxin, the mechanism of entry into the midgut epithelial cells is not currently known. As no heme transport proteins in have been identified in any dipteran, heme fluorescent analog studies were performed to visualize changes in expression caused by heme followed by global expression analyses performed in both cultured cells and midgut tissues using NGS-based RNA sequencing with the end goal to identify the gene(s) that encode the membrane bound heme import proteins responsible for heme uptake during blood digestion. A list of candidate genes for RNAi knockdown was compiled based on differential expression, expression pattern across heme treatments and number of TM domains. These genes’ function relating to heme transport was then identified through siRNA mediated knockdown and treatment with zinc mesoporphyrin to assess changes in heme uptake. A number of candidate genes were identified in 2 or more cultured cell datasets out of 4 examined. In particular, 63 candidates were identified in heme exposed midguts, 23 of which were found in at least 1 cultured cell analysis as well. However, very few highly differentially expressed genes were found in any of the analyses indicating that heme import may be controlled by a redundant system of multiple transport proteins instead of a single one. Alternatively, heme transport in Ae. aegypti could be regulated post-translationally. Knockdown of candidate genes AAEL004657 and AAEL008664 suggested a potential role in heme transport in Aag2 cultured cells, though more work is needed to localize and elucidate their specific functions.
Design of Vocal Music Education System Based on VR Technology

Field: Computer Science (Physical Sciences)

With the popularization of conceptual teaching methods such as quality education and comprehensive education in schools, China has invested more energy in art education. Especially the vocal music education of art education, under this background, its music education management has entered a new stage. Through a series of means to make teaching more scientific and standardized. Music teachers are of high importance in the traditional meaning teaching mode, and most of them teach in person. The traditional teaching mode has a single way and the content is lack of change. In a very short time, teaching will lead to poor information transmission. As a product of the information age, network teaching platform has gradually become a new way for people to obtain knowledge. With the support of modern network technology, the development and Reform in the field of education have reached a qualitative leap. In view of the singleness of traditional vocal music education methods and learning situations and the limitations of learning resources, this paper deeply discusses the influence of constructivism theory on music education based on Moodle platform. Combining music education with the teaching platform based on network environment, taking constructivism theory as the main theoretical basis, using network technology, a music education system based on Moodle platform is designed and developed. It realizes the collection, playback and real-time display of audio segments, and also has the function of vocal music scoring. This design makes vocal education lessons intuitive, interactive, and interesting.
Genome Sequence of Devriesea agamarum, Isolated from Agamid Lizards with Dermatitis

Field: Immunology and Microbiology (Life Sciences)

ABSTRACT We report the genome sequence of Devriesea agamarum strain IMP2, isolated from the liver of a female Agama impalearis . This actinobacterium is associated with septicemia and dermatitis in agamid lizards. Availability of this genome sequence will contribute to the understanding of this pathogen's virulence.
MD&A tone and stock returns

Field: Economics, Econometrics and Finance (Social Sciences)

This paper examines the impact of the MD&A (Management's Discussion and Analysis) tone on cross-sectional stock returns in the unique context of Chinese A-share companies. We find a significant and positive relationship between the MD&A tone and Chinese stock returns after controlling for quantitative financial metrics. Our results suggest that the MD&A tone provides additional value that further enhances the information quality in MD&A disclosures and market responses. Furthermore, we observe that the textual tone effect is more pronounced for firms with lower institutional ownership, lower financial transparency, in less competitive market environments, and both the information factor and the sentiment factor of MD&A tone positively affect stock returns. Thus, we provide convincing evidence that MD&A narratives can serve as an effective communication tool to facilitate disclosure and mitigate information asymmetry between corporations and investors.
Searching Question: On-line or CD-ROM?-Reply

Field: Health Professions (Health Sciences)

In Reply.

—Mr Nelson points out the existence of additional avenues to MEDLINE data, and I fully agree that CD-ROM products might contribute to the new standard of information arising in medicine. It was not my intention to review all methods by which one may make use of MEDLINE and related databases while describingPaperChase. Specifically, I have not attempted to weigh the relative merits of on-line vs CD-ROM systems. A professional biomedical librarian certainly can be an ally in identifying useful information. However, in many settings, biomedical librarians will not be available on a 24-hour-a-day basis when clinicians and other scholars may need access to the literature around-the-clock, 7 days a week. The kind of browsing that Nelson rightfully praises in the CD-ROM environment also occurs on-line and, in fact, is often very instructive both in immediate yield of desired citations and in improving technique for future searches. Nelson
Impact of electronic vaccine intelligence network application used in immunization sessions in Pune city

Field: Health Professions (Health Sciences)

Background: Millions of deaths due to various diseases have been reduced globally as well as in India since inception of vaccination programme. In order to avoid the instances of cold chain failure and ensure constant monitoring of temperature of cold chain equipment’s Electronic vaccine intelligence network (eVIN) technology was adopted by Government of India.Methods: A mixed methodology design with both qualitative as well as quantitative components were studied. This study was conducted in 16 immunization centres spread across the metropolitan Pune city in western Maharashtra.Results: Vaccine beneficiaries increased for polio 814 (3.25%), DPT1643 (21.79%), MR 9615 (23.02%) and IPV 2297 (23.58) vaccine after execution of eVIN app. Vaccine availability of BCG (6.04%), Polio (20.02%), MR (64.24%), IPV (11.38%) and hep B had increased as compared to the other vaccines. The vaccine wastage decreased for penta (8.64%), IPV (123.98%), TT (32.63%) and hep B (47%) vaccines post eVIN application compared to the pre eVIN. The qualitative study included in depth interviews, key informants’ interview and focus group discussions. Various themes and subthemes emerged like the user-friendliness of app, difficulties faced by health care workers, their suggestions for improvements in the app.Conclusions: The eVIN system is playing a pivotal role in effective and efficient management of vaccine supply, maintaining stock availability and monitoring. To reduce vaccine wastage usage of small dose vials in immunization sessions would be helpful.
Long‐Term Outcome After Resection of Huge Hepatocellular Carcinoma ≥10 cm: Single‐Institution Experience with 471 Patients

Field: Medicine (Health Sciences)

Abstract Background Tumor recurrence is very common after resection of huge hepatocellular carcinoma (HCC). This study intended to evaluate early recurrence and long‐term outcomes in patients with huge HCC ≥ 10 cm after primary resection and treatment of recurrence. Methods Recurrence and survival outcomes were retrospectively evaluated in 471 patients with huge HCCs who underwent resection between January 2000 and April 2012. Results Mean tumor diameter was 13.6 ± 3.1 cm, with 93 % of patients having single tumors. Anatomic and R0 resection rates were 91.1 and 89.4 %, respectively. Perioperative mortality rate was 1.7 %. Tumor recurrence and patient survival rates were 62.2 and 69.2 % at 1 year and 76.0 and 35.5 % at 5 years, respectively. Of patients with recurrence, 92.5 % received specific treatment. Median patient survival period after initial intrahepatic recurrence was 16 months. Tumor volume did not affect recurrence or survival outcomes. Independent risk factors for tumor recurrence and patient survival were serum alpha‐fetoprotein ≥100 ng/mL, hypermetabolic uptake on positron emission tomography, satellite nodules, and microvascular invasion. These four factors were used to develop a risk prediction model, in which 1‐year HCC recurrence rates in patients with 0, 1, 2, 3, and 4 risk factors were 18.7, 30.3, 58.7, 79.0, and 92.1 %, respectively, and their 1‐year patient survival rates were 100, 97.0, 75.5, 63.9, and 42.1 %, respectively. Conclusions In patients with huge HCCs, hepatic resection with active recurrence treatment resulted in improved long‐term survival. Our 4‐factor risk prediction model appears to contribute to quantitative postoperative risk estimation for early HCC recurrence and patient survival in patients with HCC ≥ 10 cm.
Analisis Faktor-Faktor yang Mempengaruhi Ekspor Crude Palm Oil (CPO) di Provinsi Kalimantan Selatan

Field: Economics, Econometrics and Finance (Social Sciences)

This study aims to determine the factors that affect crude palm oil exports in South Kalimantan province, knowing the influence of production amount, dollar exchange rate, and CPO price on CPO exports in South Kalimantan Province. In increasing CPO production for the future, Indonesia is still superior because of the availability of sizeable tropical land for planting oil palm trees. Indonesia's CPO production, especially in South Kalimantan, continues to increase. It can be identified because of the influence of the amount of output, dollar exchange rate, and CPO price on South Kalimantan CPO exports?This research is research that uses multiple linear regression analysis techniques. Linear regression is one of the statistical tools used to describe the relationship between variables; the dependent and independent variables are used. The results showed that the amount of production (X1)) and the exchange rate of the Dollar (X3) together did not significantly influence the CPO Export of South Kalimantan (Y).
A Magnetically Actuated Plasma Device for Ionic Wind Generation

Field: Engineering (Physical Sciences)

This study presents a novel micro electromagnetic device to enable ionic actuation. Stable atmospheric plasma was generated at a low voltage using a micro discharge device, which integrated an initiation carrier injector into the device. A high speed revolution of the plasma was realized using the Lorentz force by applying a magnetic field of a few tenths of a tesla and the angular velocity of plasma revolution was electrically measured. The proposed device yields a circular ionic motion, which will generate a new type of circular ionic wind. In addition, the electromagnetic operation allowed continuous plasma movements along the electrode, significantly improving device reliability by suppressing unsteady electrode wear and decreasing device temperature.
Non-integrated defect relation for meromorphic maps of complete Kahler manifolds encountering divisors

Field: Mathematics (Physical Sciences)

In this dissertation, we are interested in meromorphic maps of a complete Kahler manifold whose universal covering is biholomorphic to the ball in Cm into complex projective manifolds. We first give a non-integrated defect for a meromorphic map of the above Kahler manifold into Pn(C) intersecting hypersurfaces in general position and an application of this non-intetgrated defect to the Gauss map of a complete regular submanifold of Cm. We then focus on the uniqueness problem, i.e, to find how many hypersurfaces are sufficient to uniquely determine a map which intersects them. The first result provides a complement to the recent result of Min Ru on the defect relation for meromorphic mappings from Cm into Pn(C) intersecting hypersurfaces in general position and the second provides a complement to the result of H. Fujimoto on the unicity theorem for meromorphic maps of a complete Kahler manifold into Pn(C).
Pengaruh Infusa Daun Kemuning (Murraya Paniculata (L.)Jack) Terhadap Penurunan Berat Badan Mencit Galur Swiss Webster

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Saat ini, obesitas dan overweight telah menjadi masalah kesehatan yang harus dipecahkan, mengingat angka kejadiannya yang semakin meningkat. Obesitas sangat berbahaya karena dapat menimbulkan komplikasi, antara lain penyakit hipertensi, stroke, penyakit arteri koronaria, dan lain-lain. Salah satu upaya untuk menurunkan berat badan yang telah terbukti secara empiris adalah dengan terapi herbal menggunakan daun kemuning (Murraya paniculata (L.) Jack). Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian infusa daun kemuning terhadap penurunan berat badan mencit galur Swiss Webster. Penelitian ini termasuk penelitian prospektif eksperimental sungguhan dengan menggunakan Rancangan Acak Lengkap (RAL) bersifat komparatif. Dua puluh lima ekor mencit dibagi dalam lima kelompok secara acak (n=5), yaitu kelompok perlakuan I diberi infusa daun kemuning dosis 10%, kelompok II dengan dosis 20%, kelompok III dengan dosis 40% , kelompok IV diberi produk teh pelangsing X sebagai pembanding, dan kelompok V diberi aquades sebagai kontrol, setiap 3 hari sekali selama dua puluh satu hari. Data yang diukur adalah berat badan mencit dalam gram. Analisis data menggunakan metode ANAVA satu arah. Persentase perubahan rerata berat badan mencit pada
kelompok I,II, dan III menunjukkan penurunan terhadap rerata berat badan awal, namun tidak signifikan secara statistik dibandingkan kelompok kontrol (p>0.05). Kesimpulan yang diperoleh adalah pemberian infus daun
kemuning tidak terbukti menurunkan berat badan.
IL‐17A promotes neutrophilic inflammation and disturbs acute wound healing in skin

Field: Medicine (Health Sciences)

Abstract In the wound healing process, neutrophils are the first inflammatory cells to move to the wound tissues. They sterilize wounds by killing microbes, and they stimulate other immune cells to protect the host from infection. In contrast, neutrophil‐derived proteases cause damage to host tissues, so neutrophils play dual opposite roles in wound healing. Interleukin‐17A ( IL ‐17A) is a proinflammatory cytokine that promotes the recruitment of these cells. The role of this cytokine in the wound healing process is not fully clarified. In the present study, therefore, we examined how defect in IL ‐17A production affected the wound healing in skin. IL ‐17A‐knockout ( KO ) mice showed promoted wound closure, myofibroblast differentiation and collagen deposition and decreased the neutrophil accumulation compared with wild‐type ( WT ) mice. In contrast, the administration of recombinant IL ‐17A led to delayed wound closure, low collagen deposition and accelerated neutrophilic accumulation. In addition, the treatment of IL ‐17A‐administered mice with a neutrophil elastase inhibitor improved the wound repair to the same level as that of WT mice. These results indicated that IL ‐17A hampered the wound healing process and suggested that neutrophilic inflammation caused by IL ‐17A may be associated with impaired wound healing in skin.
Age‐dependent messenger RNA expression of toll‐like receptor 4 and intercellular adhesion molecule‐1 in peripheral blood mononuclear cells

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Abstract Background Inflammation plays an important role in the ageing process in which monocytes/macrophages are important players. Intercellular adhesion molecule‐1 (ICAM‐1), tumour necrosis factor‐α (TNF‐α) and Toll‐like receptor 4 (TLR4) are well‐known inflammatory markers. This study aimed to investigate the relationship between age and the expression and correlation of ICAM‐1, TNF‐α and TLR4 mRNA in peripheral blood mononuclear cells (PBMCs). Methods A total of 239 participants were recruited in a medical centre in Taiwan. The mRNA isolated from the PBMCs was used to determine the levels of ICAM‐1, TNF‐α and TLR4 mRNAs with real‐time polymerase chain reaction (PCR). The propensity‐matched analysis was also applied for subgroup analysis. Results When compared 189 older adults (≧65 years) to 50 younger adults (<65 years), the ICAM‐1, TNF‐α and TLR4 mRNA levels in PBMCs were significantly higher in older adults (2.00 ± 0.72 vs 0.87 ± 0.34 for ICAM‐1, 2.32 ± 0.69 vs 1.15 ± 0.44 for TNF‐α and 1.56 ± 0.47 vs 1.05 ± 0.51 for TLR4, and all P < .0001). Also, both age and TLR4 were independent factors affecting mononuclear cell ICAM‐1 in the multiple linear regression analysis ( P < .0001). Conclusion The mRNA levels of ICAM‐1 and TLR4 in PBMCs are higher in older adults than those in younger adults. TLR4 is an independent factor affecting ICAM‐1 expression in PBMCs, especially in older adults. This may suggest that ICAM‐1 and TLR4 in PBMCs are potential biomarkers and their relationship may shed some light on the ageing process.
Rituximab treatment for rhupus syndrome: a systematic literature review

Field: Medicine (Health Sciences)

Introduction: Rhupus is a poorly described and underdiagnosed disease in which features of both rheumatoid arthritis (RA) and systemic lupus erythematous (SLE) appear in the same patient. Herein, we present a systematic literature review to analyze the clinical, laboratory and radiological data of all reported rhupus patients treated with rituximab (RTX). Methods: This systematic review followed the preferred reporting items for systematic reviews guidelines. Eligible articles were searched in Medline, Embase, and Cochrane Library. We analyzed cohort studies, case series and case reports. Results: Four papers were finally selected for analysis. The studies were published between 2012 and 2022. Overall, 29 patients were included. All rhupus patients were previously treated with conventional Disease-Modifying Antirheumatic Drugs (DMARDs) and RTX was the first biologic DMARD prescribed in 22 patients. A significant reduction in mean the 28 Disease-activity-score (DAS28) from baseline to the end of follow-up was observed in all the studies. Mean DAS28 decreased from 5.65 to 3.65. The clinical activity of SLE was evaluated in 2 studies using The Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Overall, mean SLEDAI decreased from 6.05 at baseline to 2.66 at the end of follow-up Based on the ultrasonographic (US) disease activity assessment performed one study, US count scores progressively reduced, indicating that a US response was sustained after RTX treatment. Overall, only two severe adverse events were noted. Conclusion: In the light of this literature systematic review, RTX seems to be effective and safe in rhupus syndrome.
Quantum state manipulation and cooling of ultracold molecules

Field: Physics and Astronomy (Physical Sciences)

An increasingly large variety of molecular species are being cooled down to low energies in recent years, and innovative ideas and powerful techniques continue to emerge to gain ever more precise control of molecular motion. In this brief review we focus our discussions on two widely employed cooling techniques that have brought molecular gases into the quantum regime: association of ultracold atomic gases into quantum gases of molecules and direct laser cooling of molecules. These advances have brought into reality our capability to prepare and manipulate both internal and external states of molecules quantum mechanically, opening the field of cold molecules to a wide range of scientific explorations.
Serum oxylipins indicate subcortical ischemic vascular disease in patients with clinical stroke

Field: Medicine (Health Sciences)

Abstract Background Subcortical ischemic vascular disease (SIVD) is the most common vascular pathology found in patients with late onset dementia. The soluble epoxide hydrolase (sEH) enzyme inactivates anti‐inflammatory and vasoactive cytochrome p450 derived polyunsaturated fatty acid epoxides by converting them into cytotoxic dihydroxy (diol) oxylipin species. Previously, we found that the ratios of linoleic acid (LA) diols to epoxides were associated with more white matter hyperintensity (WMH) in patients with transient ischemic attack; however, it remains univestigated if these oxylipins can identify SIVD in patients with ischemic stroke of heterogenous cerebrovascular pathologies such as large vessel occlusion and lacunar infarcts. Method The study cohort comprises patients from the Ontario Neurodegenerative Disease Research Initiative, who has clinical stroke of either large or small vessel etiology (according to TOAST criteria). 4 LA oxylipins (12,13‐dihydroxyoctadecamonoenoic acid (12,13‐DiHOME), 12,13‐epoxyoctadecenoic acid (12,13‐EpOME), 9,10‐DiHOME, 9,10‐EpOME) were extracted from blood and quantified with a targeted ultrahigh pressure liquid chromatography mass spectrometry (UPLC‐MS/MS) WMH and perivascular spaces (PVS) were quantified from structural MRI scans using a personalized semi‐automatic processing pipeline (Lesion Explorer). The associations between oxylipins and small vessel disease markers were examined using linear regression models. Result In 80 stroke patients (n=50 large vessel disease and n=30 lacunar infarcts), the ratio of 12,13‐DiHOME to its epoxide sEH substrate 12,13‐EpOME was higher among patients with small vessel etiology (F 1,79 = 4.55, p = 0.036). In linear regression models controlling for age, sex, APOE, waist hip ratio, diabetes, and hyperlipidemia, the same ratio, and the ratio of 9,10‐DiHOME/9,10‐EpOME were positively associated with volume of deep (β = 0.338, p = 0.004; β = 0.288, p = 0.012, respectively) and periventricular (β = 0.316, p = 0.003; β = 0.400, p < 0.001, respectively) WMH. The same ratios were associated with higher volumes of perivascular spaces in the white matter (β = 0.293, p = 0.013; β = 0.276, p = 0.016, respectively) but not in the basal ganglia (β = 0.145, p = 0.212; β = 0.086, p = 0.450, respectively). Conclusion Oxylipins derived from sEH activity may contribute to cerebral small vessel disease and to stroke of small vessel etiology.