186 PROSTATE NEEDLE BIOPSIES: HISTOPATHOLOGY AND METABOLITE BIOMARKERS ON THE SAME TISSUE BIOPSY

Field: Medicine (Health Sciences)

You have accessJournal of UrologyProstate Cancer: Staging1 Apr 2011186 PROSTATE NEEDLE BIOPSIES: HISTOPATHOLOGY AND METABOLITE BIOMARKERS ON THE SAME TISSUE BIOPSY Dean Troyer, Raymond Lance, Klaus-Peter Adam, Danny Alexander, and Jeffrey Shuster Dean TroyerDean Troyer Norfolk, VA More articles by this author , Raymond LanceRaymond Lance Norfolk, VA More articles by this author , Klaus-Peter AdamKlaus-Peter Adam Durham, NC More articles by this author , Danny AlexanderDanny Alexander Durham, NC More articles by this author , and Jeffrey ShusterJeffrey Shuster Durham, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.256AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Prostate needle biopsies are the gold standard for prostate cancer diagnosis. Biopsies are analyzed by hematoxylin and eosin (H&E) histopathology for diagnosis, and for cancer aggressiveness by Gleason grade. Gleason grade is reported as a semi-quantitative, categorical variable. Small molecule metabolite biomarkers, for which measurements are more quantitative, are also useful in the determination of the aggressiveness of prostate cancer (Sreekumar et al., Nature, 457:910, 2009). These assays routinely require extraction methods which disrupt tissue architecture and, as such, preclude measuring metabolites and performing histology on the same portion of tissue. We present a new method which allows both histology and metabolomics to be performed on exactly the same tissue biopsy. The method is compatible with existing clinical and histology workflows. METHODS Prostatectomy specimens were biopsied ex vivo, and placed in an alcohol-based fixative. The biopsies were then removed from the alcohol fixative, processed, embedded in paraffin, sectioned, and stained by standard methods. The alcohol was retained and analyzed by metabolomics using a mass spectrometry-based platform (Evans et al., Anal. Chem. 81:6656–67, 2009). RESULTS The figure below shows prostate needle biopsies fixed and stained using the new method, Preservation by Extraction and Fixation (PREF), and formalin fixed biopsies as controls. The results show that the morphology and cell architecture are well defined. Immunohistochemistry results for the PIN4 cocktail (basal keratin; p63; and racemase) were also satisfactory. The alcohol extracts were analyzed by metabolomics. With the high analytical sensitivity of mass spectrometry, hundreds of metabolites and candidate biomarkers were observed from individual prostate core needle biopsies. The metabolite profiles were collected and correlated with the histopathology results. CONCLUSIONS A novel method for performing small molecule biomarker analysis while retaining tissue architecture suitable for histopathology is presented. The combination of histopathology and metabolite biomarkers on the same biopsy specimen may lead to better clinical classifications of tumor aggressiveness. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e77 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Dean Troyer Norfolk, VA More articles by this author Raymond Lance Norfolk, VA More articles by this author Klaus-Peter Adam Durham, NC More articles by this author Danny Alexander Durham, NC More articles by this author Jeffrey Shuster Durham, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...