Intracellular redistribution of nitric oxide synthase in snail neurons: role of hypothermia

Field: Neuroscience (Life Sciences)

Event Abstract Back to Event Intracellular redistribution of nitric oxide synthase in snail neurons: role of hypothermia T. Rőszer1*, É. Kiss-Tóth1, D. Rózsa1, T. Józsa2, A. J. Szentmiklósi3 and G. Bánfalvi4 1 Spanish National Cardiovascular Research Center, Department of Microbial Biotechnology and Cell Biology 2 University of Debrecen, Department of Pediatrics, Medical Health Science Center, Hungary 3 University of Debrecen, Department of Pharmacology and Pharmacotherapy, Medical and Health Science Center, Hungary 4 University of Debrecen, Department of Microbial Biotechnology and Cell Biology Neuronal nitric oxide (NO) levels are modulated through the control of NO synthase (NOS) catalytic activity. Although signals limiting excess NO synthesis are being extensively studied in the vertebrate nervous system, our knowledge is rather limited on the modulation of NOS in neurons of invertebrates. We have previously reported a transient inactivation of NOS in hibernating snails. In the present study, we investigated further the mechanism leading to blocked NO production during hypothermic periods of Helix pomatia. Our studies with intact ganglia and cultured neurons revealed that hypothermic challenge translocated NOS from the cytosol to the perinuclear endoplasmic reticulum, and that this cytosol to membrane trafficking was essential for inhibition of NO synthesis. Cold stress also downregulated NOS mRNA levels in snail neurons, although the amount of NOS protein remained unaffected in response to hypothermia. We provide evidence that hypothermia keeps NO synthesis "hibernated" through subcellular redistribution of NOS. Keywords: Molecular and cellular neurobiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Molecular and cellular neurobiology Citation: Rőszer T, Kiss-Tóth É, Rózsa D, Józsa T, Szentmiklósi AJ and Bánfalvi G (2011). Intracellular redistribution of nitric oxide synthase in snail neurons: role of hypothermia. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00025 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. T. Rőszer, Spanish National Cardiovascular Research Center, Department of Microbial Biotechnology and Cell Biology, troszer@cnic.es Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers T. Rőszer É. Kiss-Tóth D. Rózsa T. Józsa A. J Szentmiklósi G. Bánfalvi Google T. Rőszer É. Kiss-Tóth D. Rózsa T. Józsa A. J Szentmiklósi G. Bánfalvi Google Scholar T. Rőszer É. Kiss-Tóth D. Rózsa T. Józsa A. J Szentmiklósi G. Bánfalvi PubMed T. Rőszer É. Kiss-Tóth D. Rózsa T. Józsa A. J Szentmiklósi G. Bánfalvi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.