jimnoneill/paper-to-field-training huggingface dataset:
Ca2+/calmodulin-dependent protein kinase type II (CaMKII) regulates histone methylation to promote zebrafish hematopoietic stem cell specification

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Ca2+/calmodulin dependent protein kinase II (CaMKII) is an important signaling molecule involved in many developmental processes. This project aims to understand the role of CaMKII in specification of hematopoietic stem cells (HSCs) in zebrafish. HSCs undergo hematopoiesis to specify all cells that constitute blood. The process of hematopoiesis along with the specification of hematopoietic stem cells is conserved in vertebrates. CaMKII is expressed in tissues important for HSC specification, including the somites, notochord, and dorsal aorta. The dorsal aorta is the site of HSC specification in zebrafish. Injection of camk2g1 translational blocking antisense oligonucleotides (MO) or Cas9 guide RNAs targeting camk2g1 leads to a loss of HSCs. Using a pharmacological inhibitor of CaMKII, KN93, it is demonstrated that CaMKII is required for HSC specification in a time-dependent manner. Inhibition of CaMKII after 16 hours post fertilization (hpf) in zebrafish embryo does not result in HSC loss. Additionally, HSC specification can be recovered by washing out KN93 at 48 hpf but not at 72 hpf. Using whole mount in-situ hybridization (WISH) and immunolocalization, loss of CaMKII results in increased expression of the epigenetic regulator, enhancer of zeste 2 (ezh2), which trimethylates lysine 27 on histone 3 (H3K27me3). Altered methylation could lead to decreased expression of Notch ligand, Jag1a, inhibiting HSC specification. Understanding the signals that guide HSC specification is important for differentiation of HSCs from induced pluripotent stem cells in order to remedy primary immunological disorders and hematological malignancies.
Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation

Field: Neuroscience (Life Sciences)

Neural progenitor cells (NPCs) are often used to study the subcellular mechanisms underlying differentiation into neurons in vitro. Works published to date have focused on the pathways that distinguish undifferentiated NPCs from mature neurons, neglecting the earlier and intermediate stages of this process. Current evidence suggests that mitochondria interaction with the ER is fundamental to a wide range of intracellular processes. However, it is not clear whether and how the mitochondria–ER interactions differ between NPCs and their differentiated counterparts. Here we take advantage of the widely used NPC line LUHMES to provide hints on the mitochondrial dynamic trait changes that occur during the first stage of their maturation into dopaminergic-like neurons. We observed that the morphology of mitochondria, their interaction with the ER, and the expression of several mitochondria–ER contact site resident proteins change, which suggests the potential contribution of mitochondria dynamics to NPC differentiation. Further studies will be needed to explore in depth these changes, and their functional outcomes, which may be relevant to the scientific community focusing on embryonic neurogenesis and developmental neurotoxicity.
Dendrobium unicum Seidenf., Bot. Tidsskr.

Field: Agricultural and Biological Sciences (Life Sciences)

Dendrobium unicum Seidenf., Bot. Tidsskr. 65: 332, 1970 Specimens examined:— CHIN. Unspecified: fide Saw Lwin (2004: 26). SHAN. Unspecified: fide Saw Lwin (2004: 26). WITHOUT LOCALITY. Fide Y. Tanaka (2007: 33). Distribution:— Myanmar, Thailand and Indochina.
The most important predictors in hospital mortality of patients with acute ST elevation myocardial infarction (STEMI) - using Yazd Cardiovascular Diseases Registry, YCDR data

Field: Medicine (Health Sciences)

Abstract

Background One of the consequences of coronary heart diseases (CAD) is a heart attack, which can sometimes lead to death. In Iran, as well as in most West Asian countries, cardiovascular diseases are the leading cause of death, accounting for 46% of total mortality. This study aims to estimate and identify in hospital mortality predictors or prognostic factors for patients with acute ST elevation myocardial infarction (STEMI). Methods This study utilized data from the Yazd Cardiovascular Diseases Registry (YCDR), which is a registry of inpatient ischemic heart disease in Yazd province, Iran. Logistic Regression was used to analyze and adjust for confounding. Variables that were not significant in the univariate analysis (P_value > 0.10) were discarded, and the remaining variables were entered into multivariable analysis. Variables with P_value < 0.05 were retained in the model, while others were discarded. Results The study included 1,861 patients with STEMI. Among them, 105 (5.7%) individuals died during admission the hospital. After multivariable logistic regression, the following variables were identified as prognostic factors for in-hospital mortality of STEMI: having a history of CVA (OR: 23.1, 95% CI: 19.2–28.3), lower ejection fraction (OR: 8.6, 95% CI: 7.2–9.8), lower HDL cholesterol (OR: 1.1, 95% CI: 1.01–2.3), being single (OR: 1.4, 95% CI: 1.2–3.1), and lower hemoglobin (OR: 1.4, 95% CI: 1.3–2.9). Conclusion We evaluated the most important predictors of in-hospital mortality of STEMI and found that lower ejection fraction, lower hemoglobin levels, having a history of CVA, being unmarried, and low HDL cholesterol levels are important prognostic factors. Health policy in STEMI management must consider these factors to improve hospital prognosis.
Investigation of Atypical Bacteria and Virus Antigens in Respiratory Tract Infections by Use of an Immunofluorescence Method

Field: Medicine (Health Sciences)

In this study an immunofluorescence (IF) method was used to investigate the antigens of viruses and atypical bacteria in respiratory tract infections (RTI) in pediatric and adult age groups. In this prospective study of 2 years (1998-2000), IF was used to investigate the antigens of 7 viral and 3 atypical bacteria to be used for the etiological diagnosis of RTI. Sputum (33.6%) and nasopharyngeal aspirate specimens were obtained from pediatric patients (Group I, 76 cases) and adults (Group II, 135 cases) with RTI symptoms. Antigen detection rates were found to be 44.7% in Group I and 67.4% in Group II (P < 0.05). The following rates for specific antigens in Groups I and II, respectively, were as follows: Chlamydia pneumoniae, 17.1 and 13.3% (P > 0.05); Mycoplasma pneumoniae, 0 and 9.6% (P < 0.05); influenza A virus, 3.9 and 16.3% (P < 0.05); adenovirus, 3.9 and 14.8% (P < 0.05); parainfluenza virus type 1, 5.3 and 7.4% (P > 0.05); respiratory syncytial virus, 9.2 and 1.5% (P < 0.05); parainfluenza virus type 2, 3.9 and 3%(P > 0.05); and influenza B virus, 1.3 and 1.5% (P > 0.05). Mixed agents were found at a rate of 2.6 and 3.7% (P > 0.05) in Groups I and II, respectively. Parainfluenza virus type 3 and Legionella pneumophila antigens were not found. Since detecting etiological agents provides an important guide for determining the most appropriate antibiotic therapy, this IF method could be applied in clinical practice for arriving at a correct diagnosis and administration of effective treatment.
The response of paediatric arrhythmias to intravenous and oral flecainide.

Field: Medicine (Health Sciences)

Flecainide acetate was administered intravenously and orally to 12 consecutive children, aged 1-15 years, presenting with arrhythmias that were life threatening or resistant to conventional medical treatment. Three children had arrhythmias related to Wolff-Parkinson-White syndrome, four had concealed accessory pathways, two had His bundle tachycardia, and three had ventricular tachycardia. Of seven patients who were given flecainide intravenously, four returned to sinus rhythm and in a fifth successful rate control of His bundle tachycardia was achieved. All 12 patients were given the drug orally: in nine it was successful in preventing recurrence of arrhythmia, in one satisfactory rate control was achieved, and in two it was withdrawn because it produced more frequent attacks of tachycardia. No other adverse effects occurred. The efficacy and low toxicity of treatment in this study suggests that flecainide acetate may have an important role in the management of selected paediatric arrhythmias.
Cilengitide, an αvβ3-integrin inhibitor, enhances the efficacy of anti-programmed cell death-1 therapy in a murine melanoma model

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Integrins play an important role in multiple stages of tumor progression and metastasis. Previous studies have shown synergistic effects of combined αvβ6-integrin and αvβ8-integrin inhibitors with immunotherapy. However, the role of αvβ3-integrin inhibitor in tumor immunity is still unclear. In this study, we aimed to elucidate the impact of the αvβ3-integrin inhibitor on PD-L1 expression and sensitivity to immune checkpoint blockade in melanoma. We investigated the effects of cilengitide, an αvβ3-integrin inhibitor, on cell viability and apoptosis of melanoma cell lines. And we explored how cilengitide regulated the expression of PD-L1 in melanoma cells in vitro and in vivo, using immunofluorescence, flow cytometry, Western blotting, and immunohistochemistry. A subcutaneous B16 murine melanoma model was utilized to determine whether combining cilengitide with anti-PD1 therapy inhibited tumor growth and positively regulated tumor microenvironment (TME). Our results showed that cilengitide inhibited cell viability and induced apoptosis in B16 and A375 cell lines. Furthermore, cilengitide decreased PD-L1 expression by reducing STAT3 phosphorylation in two melanoma cell lines. Cilengitide also reduced subcutaneous tumor PD-L1 expression in the B16 murine melanoma model. Accordingly, cilengitide positively regulated antitumor immune responses and provided durable therapy when combined with anti-PD1 monoclonal antibody in the murine melanoma model. This combination therapy reduced tumor growth and extended survival. Our study highlights that cilengitide enhances the efficacy of anti-PD1 therapy and produces a stronger antitumor immune response. This combination therefore represents a novel therapeutic regimen that may improve immunotherapy treratment.
Pengaruh Penerapan Environmental Management Accounting Terhadap Firm Value Pada Perusahaan Manufaktur di Tangerang Raya

Field: Business, Management and Accounting (Social Sciences)

This research study aims to examine the application of environmental management accounting in increasing firm value in Tangerang Raya. This research consists of a population of 2,579 Banten provincial companies spread across Cilegon City, Serang Regency and City, Pandeglang City, Lebak City and Tangerang Raya. The samples were selected using purposive sampling with quantitative methods. Based on the multiple linear regression testing that has been carried out, the following results are obtained: there is a significant positive effect between the material input aspect and the environmental complaint mechanism aspect on firm value. Furthermore, the results of non-output aspects of products and aspects of compliance have a significant negative effect on firm value. However, there are three aspects of the proxy for the application of environmental management accounting which consist of transportation aspects, other aspects, and supplier assessment aspects that have a positive and insignificant effect on firm value.
Improvement in Survival and Cardiac Metabolism After Gene Transfer of Sarcoplasmic Reticulum Ca 2+ -ATPase in a Rat Model of Heart Failure

Field: Medicine (Health Sciences)

Background — In heart failure, sarcoplasmic reticulum (SR) Ca 2+ -ATPase (SERCA2a) activity is decreased, resulting in abnormal calcium handling and contractile dysfunction. We have previously shown that increasing SERCA2a expression by gene transfer improves ventricular function in a rat model of heart failure created by ascending aortic constriction. Methods and Results — In this study, we tested the effects of gene transfer of SERCA2a on survival, left ventricular (LV) volumes, and metabolism. By 26 to 27 weeks after aortic banding, all animals developed heart failure (as documented by >25% decrease in fractional shortening) and were randomized to receive either an adenovirus carrying the SERCA2a gene (Ad.SERCA2a) or control virus (Ad.βgal-GFP) by use of a catheter-based technique. Sham-operated rats, uninfected or infected with either Ad.βgal-GFP or Ad.SERCA2a, served as controls. Four weeks after gene transfer, survival in rats with heart failure treated with Ad.βgal-GFP was 9%, compared with 63% in rats receiving Ad.SERCA2a. LV volumes were significantly increased in heart failure (0.64±0.05 versus 0.35±0.03 mL, P <0.02). Overexpression of SERCA2a normalized LV volumes (0.46±0.07 mL) in the failing hearts. 31 P NMR analysis showed a reduced ratio of phosphocreatine to ATP content in failing+Ad.βgal-GFP compared with sham+Ad.βgal-GFP (0.82±0.13 versus 1.38±0.14, P <0.01). Overexpression of SERCA2a in failing hearts improved the phosphocreatine/ATP ratio (1.23±0.28). Conclusions — In this study, we show that unlike inotropic agents that improve contractile function at the expense of increased mortality and worsening metabolism, gene transfer of SERCA2a improves survival and the energy potential in failing hearts.
Moments of Cooperation and Incorporation: African American and African Jamaican Connections, 1782-1996. By Erna Brodber

Field: Social Sciences (Social Sciences)

Animating Erna Brodber’s study is the question of whether the collective experience of African-descended peoples could form a structure that would protect the interests of all those who comprise the black Atlantic. Brodber seeks to answer this question by examining six moments of interaction between African Americans and African Jamaicans from the late eighteenth century to the late twentieth century. Despite these moments of cooperation and incorporation, Brodber concludes that, over time, connections between these two groups of diasporic blacks have “grown slack,” leaving little hope for the emergence of mutually protective political or institutional structures in the black Atlantic (149). The book is divided into six chronologically organized, thematic chapters. Chapter 1 examines the fate of enslaved blacks brought to Jamaica at the end of the American Revolution by their owner, James Wright, the last governor of Georgia. Resettled on Wright’s Palmetto estate in the parish of St. Mary, these enslaved African Americans were intended to be Wright's financial support, a policy endorsed by the British government. While many of the enslaved perished due to starvation and illness, Brodber discusses the process by which those who survived were incorporated socially and culturally into the existing enslaved and free Jamaican population. Using a combination of genealogical research and oral interviews, Brodber traces the descendants of these two groups into the present. She finds that while it is possible to identify distinct cultural retentions, incorporation and time has “blurred” the memory of this early nineteenth century black trans-Atlantic connection (149).
Laminin-5 and hemidesmosomes: role of the α3 chain subunit in hemidesmosome stability and assembly

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

ABSTRACT Hemidesmosomes are complex macromolecular structures which integrate elements of the extracellular matrix and the cytoskeleton of epithelial cells. To characterize cell-matrix interactions in the hemidesmosome, we have made use of 804G cells which possess the unusual ability to assemble hemidesmosomes in vitro. During the course of our studies, we have raised a set of monoclonal antibodies against rat laminin-5, the major structural element comprising 804G matrix. One of these, termed CM6, recognizes the 150 kDa α chain of rat laminin-5 and binds the globular (G) domain of intact laminin-5 molecules as determined by rotary shadowing. CM6 antibodies perturb formed hemidesmosomes in 804G cells. In particular, within 1 hour of incubation of 804G cells with CM6 antibodies, colocalization of laminin-5 and α6β4 integrin is lost and by 2 hours, staining generated by hemidesmosomal antibodies appears primarily cytoplasmic in the perinuclear zone. Ultrastructurally, CM6 antibodies first appear to induce detachment of hemidesmosomes from the underlying matrix. Next, portions of the basal cell surface invaginate to form vesicles whose cytoplasmic-facing surface is coated with hemidesmosomes still associated with keratin intermediate filaments. Anchoring filaments extend into the inside compartment of the vesicles. We have also studied the impact of CM6 antibodies on a model system in which the matrix of 804G cells induces de novo assembly of hemidesmosomes in human keratinocytes. This process involves the plasma membrane reorganization of the hemidesmosome associated integrin α6β4 as well as a redistribution of other hemidesmosome components such as the 230 kDa bullous pemphigoid antigen. Pretreatment of 804G matrix with CM6 antibodies blocks such plasma membrane reorganization of hemidesmosome components and inhibits hemidesmosome formation. Our studies indicate a crucial role for the G domain of the α chain of laminin-5 in both nucleation of hemidesmosome assembly as well as maintenance of hemidesmosome structural integrity.
The Effect of Population, Human Development Index, and Gross Regional Domestic Product on Poor Population

Field: Social Sciences (Social Sciences)

Purpose: This study aims to determine the effect of population, Human Development Index and Gross Regional Domestic Product on the number of poor people in Minahasa District Theoretical Framework: The data used in this study uses secondary data sourced from the Central Bureau of Statistics for North Sulawesi Province in 2011-2021. This type of research is quantitative research. Design/Methodology/Approach: The analysis technique used in this study is panel data regression analysis. Findings: The results showed that population, Human Development Index, and Gross Regional Domestic Product have an effect on the number of poor people. The population number and Human Development Index variables have a positive and significant effect on the number of poor people. The Gross Regional Domestic Product variable has a negative and significant effect on the population Research Practical and Social Implications: The Gross Regional Domestic Product variable has a negative and significant effect on the population. Originality/Value: Partially the population has a positive and significant effect on the numberpoor people in Minahasa, North Minahasa, South Minahasa,Southeast Minahasa in 2012-2021.
Danagla Traders of Northern Sudan: Rural capitalism and agricultural development

Field: Social Sciences (Social Sciences)

Journal Article Danagla Traders of Northern Sudan: Rural capitalism and agricultural development Get access El Haj Bilal Omer, Danagla Traders of Northern Sudan: Rural capitalism and agricultural development. Ithaca Press, London, 1985. 105 pp. £14.95 hardback. ISBN 0 86372 029 3. WENDY JAMES WENDY JAMES University of Oxford Search for other works by this author on: Oxford Academic Google Scholar African Affairs, Volume 88, Issue 351, April 1989, Pages 302–303, https://doi.org/10.1093/oxfordjournals.afraf.a098182 Published: 01 April 1989
Operational Mechanism of Competitive Procurement of General Equipment Maintenance Material

Field: Decision Sciences (Social Sciences)

In order to effectively improve the general equipment maintenance procurement management efficiency, to improve economic efficiency of using procurement funds under the premise of maintaining the same management system, to reform the operating system to one of the most effective means this paper presents preliminary ideas based on realities and analyzes the overall reform objectives and basic principles for operating mechanism reform through process analysis, goal setting, comprehensive structure. And then we points out the direction for operating mechanism reform. In the end, we argue that the operating mechanism reform is a system engineering related to many factors, so we should establish a scientific, efficient, standardized, fair, uncorrupted operating mechanism of competitive equipment procurement based on multi-party trust and co-ordination.
Spectroscopic Detection of Critical Compression of Carbon Dioxide Confined in an Nanoporous Aerogel by Coherent Anti-Stokes Raman Spectroscopy

Field: Chemistry (Physical Sciences)

The compaction of carbon dioxide in the pores of nanoporous silicon aerogel at near-critical temperatures has been studied by coherent anti-Stokes light scattering (CARS) spectroscopy. The density was determined by the shift of the vibrational line at 1388 cm $${}^{-1}$$ under isochoric heating from the subcritical temperature of 25.2 $${}^{\circ}$$ C to the supercritical one of 31.95 $${}^{\circ}$$ C. It was found that the density of carbon dioxide in nanopores near the critical temperature increases, exceeding the average value in the cuvette by about $${\sim}20\%$$ .
COMMENTS ON BURCH's CRITICISM OF GITTER ET AL's ARTICLE

Field: Medicine (Health Sciences)

To the Editor.

—The angiographic differentiation between fluorescence and pseudofluorescence is often difficult and best made by observing the lesion over a period of time. True fluorescence or leakage of dye usually increases with time, whereas pseudofluorescence parallels normal background fluorescence for five to ten minutes and then begins to fade, as in Gass' article, "Pathology of Disciform, Detachment of Neuroepithelium" (Amer J Ophthal63:592, 1967). Figure 6 of our article shows a loss of overlying retinal pigment epithelium at the site of choroidal rupture without true fluorescence. Nevertheless, lesions of this type, ie, choroidal rupture or angiod streaks, as in Fig 9 and 10 of Smith et al's article (Brit J Ophthal48:517 [Oct] 1964), may demonstrate progressive staining with fluorescein, depending on whether or not there is extravascular leakage of dye from the choroidal vasculature. Pseudofluorescence is observed whenever the overlying pigment epithelium is thinned, thereby
Improving Health Care by Help of Internet of Things and Bigdata Analytics and Cloud Computing

Field: Health Professions (Health Sciences)

Present days the devices embedded with the technology playing a vital role. All the user various kinds of information such as medical information, bank account information, user Check-in etc., were storing over various servers. The main problem with this is data storage over different servers is how much the data is being securely stored. Is our data is stored in an encrypted manner over the servers etc., such type of questions were in the users as well as cooperatives mind. Big data is a concept used to extract the lot of information from the available data. The main agenda of our paper is to collect and analyze the tribal people health information and perform the treatment with less price and time saving manner and Prediction of the disease and the drug cases with the help of patients based on past data for various seasons for various diseases.