MP49-09 REGENERATING ISLET-DERIVED FAMILY, MEMBER 4 ENHANCES CELL PROLIFERATION, CASTRATION-RESISTANCE, AND CHEMORESISTANCE THROUGH ACCELERATION OF NEUROENDOCRINE DIFFERENTIATION IN HUMAN PROSTATE CANCER CELLS

Field: Medicine (Health Sciences)

You have accessJournal of UrologyProstate Cancer: Basic Research IV1 Apr 2014MP49-09 REGENERATING ISLET-DERIVED FAMILY, MEMBER 4 ENHANCES CELL PROLIFERATION, CASTRATION-RESISTANCE, AND CHEMORESISTANCE THROUGH ACCELERATION OF NEUROENDOCRINE DIFFERENTIATION IN HUMAN PROSTATE CANCER CELLS Jun Teishima, Koichi Shoji, Keisuke Goto, Ryoken Yamaoka, Hiroyuki Shikuma, Hirotaka Nagamatsu, Kiyotaka Oka, Shunsuke Shinmei, Hiroyuki Kitano, Shinya Ohara, Tetsutaro Hayashi, Norihide Oue, Wataru Yasui, and Akio Matsubara Jun TeishimaJun Teishima , Koichi ShojiKoichi Shoji , Keisuke GotoKeisuke Goto , Ryoken YamaokaRyoken Yamaoka , Hiroyuki ShikumaHiroyuki Shikuma , Hirotaka NagamatsuHirotaka Nagamatsu , Kiyotaka OkaKiyotaka Oka , Shunsuke ShinmeiShunsuke Shinmei , Hiroyuki KitanoHiroyuki Kitano , Shinya OharaShinya Ohara , Tetsutaro HayashiTetsutaro Hayashi , Norihide OueNorihide Oue , Wataru YasuiWataru Yasui , and Akio MatsubaraAkio Matsubara View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1110AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail Introduction and Objectives We previously reported a regenerating islet-derived family, member 4 (Reg IV) as a prognostic marker of prostate cancer after radical prostatectomy. The aim of the present study was to investigate the molecular mechanism of Reg IV in castration-resistant prostate cancer (CRPC) cells. Methods Clonal lines of PC-3 and LNCaP stably transfected with a pCDNA3-expressing vector bearing Reg IV cDNA, consisting of PC-3-Reg and LN-Reg, respectively, were established. The effects of Reg IV on sensitivity to chemotherapeutic agents of prostate cancer cells were then examined by MTT assay. The expressions of molecules linked to cell proliferation, invasion, chemosensitivity, and neuroendocrine differentiation were investigated using Western blotting. The serum concentration of Reg IV in 37 prostate cancer patients, consisting of 26 patients with androgen-naive prostate cancer and 11 with CRPC, and 14 control individuals was measured by ELISA assay. Results Expressions of chromogranin A, neuron-specific enolase (NSE), and synaptophysin were induced in both PC-3-Reg and LN-Reg. Furthermore, a decreased expression of E-cadherin and a significant enhancement of cell viability (p=0.009) under androgen-deprivation conditions were demonstrated in LN-Reg. The cell viability of PC-3-Reg was significantly higher than that of PC-3 transfected with the vector alone (PC-3-vector). There were no suppressive effects in PC-3-Reg treated with docetaxel and 5-fluorouracil at concentrations sufficient to suppress cell proliferation of the PC-3-vector. The serum Reg IV level in CRPC patients was significantly higher than in the androgen-naive prostate cancer patients (p=0.026) and in the control individuals (p=0.0026). Conclusions Reg IV might enhance cell proliferation and chemoresistance through acceleration of neuroendocrine differentiation in prostate cancer cells and is thus a potential candidate for a novel biomarker of CRPC. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e505 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Jun Teishima More articles by this author Koichi Shoji More articles by this author Keisuke Goto More articles by this author Ryoken Yamaoka More articles by this author Hiroyuki Shikuma More articles by this author Hirotaka Nagamatsu More articles by this author Kiyotaka Oka More articles by this author Shunsuke Shinmei More articles by this author Hiroyuki Kitano More articles by this author Shinya Ohara More articles by this author Tetsutaro Hayashi More articles by this author Norihide Oue More articles by this author Wataru Yasui More articles by this author Akio Matsubara More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...