Dual imaging of cerebral blood flow and intracellular pH changes associated with cortical spreading depression

Field: Neuroscience (Life Sciences)

Event Abstract Back to Event Dual imaging of cerebral blood flow and intracellular pH changes associated with cortical spreading depression Eszter Farkas1* and Tihomir P. Obrenovitch2 1 Department of Physiology, University of Szeged, Hungary 2 School of Life Sciences, University of Bradford, United Kingdom Cortical spreading depression (CSD) are propagating waves of cellular depolarization that are associated with classical migraine and acute brain lesions. A multimodal imaging strategy was developed to visualize CSD-associated cerebral blood flow (CBF) changes, and the corresponding intracellular pH shifts indicative of ionic and metabolic responses. Within a cranial window prepared in halothane-anesthetized rats, repeated CSD were elicited by topical application of high-K+ medium. Two separate CCD cameras, synchronized to suitable illumination, were used for the dual imaging of changes in CBF (laser speckle contrast imaging) and Neutral red fluorescence (NR; a pH-sensitive vital dye, administered i.p.). Laser speckle CSD signatures displayed features similar to laser Doppler flowmetry data obtained previously; i.e. an initial, short CBF drop, preceding a marked hyperemia, followed by a long-lasting hypoperfusion. The initial CBF drop was most pronounced close to the CSD elicitation site (61.69±18.8% of baseline at 1.24±0.3mm; mean±stdev), and less obvious further from CSD initiation (87.4±14.9% of baseline at 2.68±0.1mm; mean±stdev), while the degree of hyperemia was similar at these two locations (144.1±26.7% near and 150.6±19.2% distant from CSD elicitation). CSD-related NR fluorescence reflected a biphasic intracellular acidification: i.e. an initial, sharp peak followed by a longer-lasting acidification. The initial sharp acidification preceded the peak of hyperemia; the latter increase in NR fluorescence developed 80 to120 sec after the hyperemia peak. We propose that the initial intracellular acidification corresponds to CSD-related, non-specific disruption of trans-membrane ionic gradients. In contrast, the long-lasting acidification may reflect an imbalance between oxygen demand and oxygen blood supply. This study was supported by a grant from the Wellcome Trust (079430/A/06/Z). Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Pathophysiology and neurology - degenerative disorders Citation: Farkas E and Obrenovitch TP (2009). Dual imaging of cerebral blood flow and intracellular pH changes associated with cortical spreading depression. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.150 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 Mar 2009; Published Online: 05 Mar 2009. * Correspondence: Eszter Farkas, Department of Physiology, University of Szeged, Szeged, Hungary, eszter.farkas.szeged@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Eszter Farkas Tihomir P Obrenovitch Google Eszter Farkas Tihomir P Obrenovitch Google Scholar Eszter Farkas Tihomir P Obrenovitch PubMed Eszter Farkas Tihomir P Obrenovitch Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.