jimnoneill/paper-to-field-training huggingface dataset:
Abstract A57: Dual role of the histone methyltransferase PR-SET7/SETD8 in the local regulation of Pol II promoter-proximal pausing

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Abstract Tumor suppressor gene silencing in cancers is often achieved through epigenetic mechanisms which involve the hypermethylation of promoter-associated CpG islands and a local shift from permissive to repressive histone modifications. Recently, we showed that epigenetic silencing of the tumor suppressor gene TMS1/ASC in human breast cancer cells is accompanied by a local shift from histone H4 lysine 16 acetylation (H4K16Ac) to H4 lysine 20 trimethylation (H4K20me3). H4K16Ac and H4K20me3 were found to play antagonistic roles in the regulation of RNA polymerase II promoter-proximal pausing, a critical post-initiation regulatory step that controls the rate of release of initiated Pol II into productive elongation and ultimately transcriptional output. We found that H4K16Ac, mediated by the histone acetyltransferase hMOF and acting in the MSL complex, facilitates the release of paused Pol II by promoting the recruitment of the BRD4/pTEFb complex whereas the local deposition of H4K20me3, mediated by the histone methyltransferase SUV420H2, enforces the pause and promotes gene repression by inhibiting the recruitment of MSL and subsequent H4K16Ac. However, how the local balance between hMOF-mediated H4K16Ac and SUV420H2-mediated H4K20me3 is achieved and converge to regulate Pol II pausing remains unclear. Here, we demonstrate that the histone methyltransferase PR-SET7/SETD8, which monomethylates histone H4 lysine 20 (H4K20me1), controls both H4K16Ac and H4K20me3, and as a result, regulates Pol II pausing and release. Specifically, we show that PR-SET7 and/or H4K20me1 is necessary for the local recruitment of the MSL complex, subsequent H4K16Ac and release from pausing. Although dispensable for SUV420H2 recruitment, PR-SET7 is required for SUV420H2-mediated H4K20me3, playing a role in enforcing Pol II pausing and subsequent epigenetic gene silencing. Whereas depletion of SUV420H2 is sufficient to deplete H4K20me3 and relieve H4K20me3-mediated Pol II pausing, pausing is maintained in the absence of PR-SET7 and local H4K20me1 due to a failure to recruit the MSL complex. These findings highlight the requirement for PR-SET7 and H4K20me1 in establishing both the H4K16Ac and H4K20me3 marks and points to its dual role in the regulation of Pol II pausing dynamics. Furthermore, our data suggests that the reversible repression imposed by the local conversion of H4K20me1 to H4K20me3 is a critical intermediate step in the epigenetic silencing of tumor suppressor genes in cancers. Citation Format: Priya Kapoor, Paula M. Vertino. Dual role of the histone methyltransferase PR-SET7/SETD8 in the local regulation of Pol II promoter-proximal pausing. [abstract]. In: Proceedings of the AACR Special Conference on Chromatin and Epigenetics in Cancer; Jun 19-22, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2013;73(13 Suppl):Abstract nr A57.
Iron-tumor cell interaction and regulation of Ca2+ homeostasis: their implication in tumor growth.

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Using [59Fe] ferric lactate, a direct relationship between iron concentration and [59Fe] uptake by Ehrlich ascites tumor cells was found. Deferoxamine and albumin inhibited this uptake. Electrophoresis showed that both molecules complexed iron from ferric lactate. [45Ca] uptake in the presence of ferric lactate showed the same inhibition, and an iron mass-dependence, these findings suggest an iron--cell membrane interaction as the cause of this phenomenon. The implication of iron--tumor cell membrane interaction in tumor growth regulation is discussed.
SEAT BELT USE INTENTION AMONG BRAZILIAN UNDERGRADUATE STUDENTS/ INTENCIÓN DE USO DEL CINTURÓN DE SEGURIDAD EN ESTUDIANTES UNIVERSITARIOS BRASILEÑOS/ INTENÇÃO DE USO DO CINTO DE SEGURANÇA EM ESTUDANTES UNIVERSITÁRIOS BRASILEIROS

Field: Psychology (Social Sciences)

The objectives of this study were to explore selfreported seat belt use and group differences in different scenarios in a Brazilian sample and research the variables related to it. 120 college students answered a questionnaire with variables from the theory of planned behavior in order to evaluate the intention of seat belt use among car occupants. Results indicated that attitude and intention were the variables that most contributed to explaining seat belt use. Intention was highly correlated with seat belt use, and attitude was correlated with intention. Seat belt use was more frequent among drivers and taxi passengers, but not among front and rear seat passengers. The article discusses the implications of the findings for possible interventions to encourage the use of seat belts among drivers and passengers.
SARS-CoV-2 NSP2 as a Potential Delivery Vehicle for Proteins

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

The development of biomolecule delivery systems is essential for the treatment of various diseases such as cancer, immunological diseases, and metabolic disorders. For the first time, we found that SARS-CoV-2-encoded nonstructural protein 2 (NSP2) can be secreted from the cells, where it is synthesized. Brefeldin A and H89, inhibitors of ER/Golgi secretion pathways, did not inhibit NSP2 secretion. NSP2 is likely secreted via an unconventional secretory pathway. Moreover, both secreted and purified NSP2 proteins were able to traverse the plasma membrane barrier and enter both immortalized human umbilical vein endothelial cells and tumor cell lines. After entry, the NSP2 protein was localized in only the cytoplasm. Cytochalasin D, a potent inhibitor of actin polymerization, inhibited the entry of NSP2. NSP2 can carry other molecules into cells. Burkholderia lethal factor 1, a monomeric toxin from the intracellular pathogen Burkholderia pseudomallei, has demonstrated antitumor activity by targeting host eukaryotic initiation translation factor 4A. An NSP2-BLF1 fusion protein was translocated across the cellular membranes of Huh7 cells and mediated cell killing. By using different approaches, including protein purification, chemical inhibition, and cell imaging, we confirm that NSP2 is able to deliver heterologous proteins into cells. NSP2 can act as a potential delivery vehicle for proteins.
[Hemodynamic profile of patients with normal coronary cineangiography].

Field: Medicine (Health Sciences)

The authors reviewed 1474 consecutive selective coronary arteriograms performed on patients with suspected coronary insufficiency for the diagnosis of obstructive coronary disease and found 281 (19.1%) cases of apparently normal coronary arteries. These patients presented mean age of 47 +/- 10 years; they were 135 (48%) males and 146 (52%) females. The objective of this study was to obtain the hemodynamic profile of these patients for the following parameters: a) aortic and left ventricular pressures; b) volumes, ejection fraction, segmentary contraction, wall thickness and mass of left ventricle; c) morphology, mobility and competence of the mitral valve. Eight groups of patients were selected: 1) without hemodynamic alterations - 18.9%; 2) with systemic arterial hypertension - 48.7%; 3) with abnormal myocardial contraction - 16.7%; 4) with idiopathic left ventricular hypertrophy - 6.4%; 5) with mitral valve prolapse - 2.5%; 7) with myocardial bridge of the left anterior descending coronary artery - 1.8%; 8) with coronary arterial microfistula of the left ventricle - 0.4%. It is desirable to determine before situations of cardiac emergencies, whether provoked ischemia, as detected by noninvasive stress testing, is present before the performing coronary arteriography in patients, specially females, with systemic arterial hypertension, left ventricular hypertrophy, disorders of ventricular contraction or mitral valve prolapse.
Barnett continent intestinal reservoir

Field: Medicine (Health Sciences)

PURPOSE: Since 1988, surgeons at five hospitals have been performing the Barnett continent intestinal reservoir (BCIR). The BCIR includes modifications to the original Kock pouch, designed to reduce the incidence of valve slippage and fistula formation. Principle modifications include an intestinal collar, an isoperistaltic valve, and a lateral pouch design. METHOD: This unique collaborative study includes 510 ulcerative colitis or familial polyposis patients, with a follow-up time from one to five years postoperatively. RESULTS: Ninety-two percent still have functioning reservoirs. Six and one-half percent have had their pouches removed and replaced with conventional Brooke ileostomies. Reoperation rate for major pouch-related complications (other than pouch removal) was 12.8 percent. These complications included slipped valve (6.3 percent), valve fistulas (4.5 percent), and pouch fistulas (6.3 percent). Several questions were administered to patients whose responses revealed a significant improvement in general quality of life, state of mind, and overall health. CONCLUSIONS: The BCIR represents a successful alternative to patients with a conventional Brooke ileostomy or those who are not candidates for the ileal pouch-anal anastomosis.
Residue Decline Dynamics of Etoxazole in Orange and Soil by UPLC-MS/MS

Field: Agricultural and Biological Sciences (Life Sciences)

A rapid,accurate and sensitive method has been developed to determine etoxazole residues in orange and soil by UPLC-MS/MS.The decline dynamics of etoxazole residues in orange and soil were studied under the conditon of field experiment.The samples were extracted by acetonitrile,followed by clean-up on silica gel cartridge and analyzed by UPLC-MS/MS.External reference method was used for quantitative determination.The linearity was satisfied(r=0.998 9) in the concentration range of 0.002-1 mg/L.The limit of detection was 6.0×10-6 μg,and the limit of quantification in orange and soil was 0.002 mg /kg.The average fortified recoveries varied from 87.3% to 98.4% with the coefficient of variation of 5.5%-8.6%.The study of residue dynamics indicated that the curve of decline accorded with the first-order kinetics equation,and the half-life of etoxazole in orange and soil were 5.6-7.6 d and 5.3-8.6 d,respectively.
Analysis of mortality of users treated at a Mobile Emergency Care Service: an observational study, Paraná, 2019-2020

Field: Health Professions (Health Sciences)

Abstract Objective To analyze the mortality of users attended by a Mobile Emergency Care Service located in Paraná. Methods This is a cross-sectional analytical study based on service reports from 2019 and 2020. The Mobile Emergency Care Service covered 21 municipalities, divided into hubs A and B. The dependent variables defined were death and time of care. Survival functions were calculated using the Kaplan-Meier estimator and the log-rank test; hazard ratio (HR) for death, by Cox regression with 95% confidence interval (95%CI). Results The study evaluated 13,326 instances of care provided. Of these, 246 resulted in death. The risk of death was higher in time-sensitive calls (HR 0.17; 95%CI 0.008; 0.37), in 2020 (HR 2.09; 95%CI 1.39; 3.16), attended by advanced support (HR 21.51; 95%CI 12.61; 36.70) and in Hub B (HR 4.26; 95%CI 2.53; 7.17). Conclusion Mortality was higher in cases that had a long wait for time-sensitive calls, occurred in less populated regions, and were dealt with by advanced support in 2020.
Henry G. Schwarz: The minorities of Northern China: a survey. (Studies on East Asia, Vol. 17.) xii, 309 pp. Washington: Center for East Asian Studies. Western Washington University, 1984. $25.

Field: Social Sciences (Social Sciences)

Henry G. Schwarz: The minorities of Northern China: a survey. (Studies on East Asia, Vol. 17.) xii, 309 pp. Washington: Center for East Asian Studies. Western Washington University, 1984. $25. - Volume 49 Issue 1
Studies on the Exergy Transfer Law for the Irreversible Process in the Waxy Crude Oil Pipeline Transportation

Field: Energy (Physical Sciences)

With the increasing demand of oil products in China, the energy consumption of pipeline operation will continue to rise greatly, as well as the cost of oil transportation. In the field of practical engineering, saving energy, reducing energy consumption and adapting to the international oil situation are the development trends and represent difficult problems. Based on the basic principle of non-equilibrium thermodynamics, this paper derives the field equilibrium equations of non-equilibrium thermodynamic process for pipeline transportation. To seek the bilinear form of “force” and “flow” in the non-equilibrium thermodynamics of entropy generation rate, the oil pipeline exergy balance equation and the exergy transfer pipeline dynamic equation of the irreversibility were established. The exergy balance equation was applied to energy balance evaluation system, which makes the system more perfect. The exergy flow transfer law of the waxy oil pipeline were explored deeply from the directions of dynamic exergy, pressure exergy, thermal exergy and diffusion exergy. Taking an oil pipeline as an example, the influence factors of exergy transfer coefficient and exergy flow density were analyzed separately.
Microenvironment Self-Adaptive Ce-Ag-Doped Mesoporous Silica Nanomaterials (CA@MSNs) for Multidrug-Resistant Bacteria-Infected Diabetic Wound Treatment

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Chronic wound healing remains a major challenge in diabetes management due to prolonged inflammation, autonomic neuropathy, and bacterial infections. In particular, multidrug-resistant bacterial infections are important to the development of diabetic wounds, leading to persistent inflammation and delayed healing. To address this issue, we developed a self-adaptive nanozyme designed to modulate infectious and inflammatory microenvironments by doping Ce and Ag into mesoporous silicon nanomaterials (MSNs). The resulting CA@MSNs exhibited strong bacterial capture capabilities via electrostatic attraction. Additionally, the synergistic effects of Ce and Ag endowed CA@MSNs with peroxidase (POD)-like activity, enabling the generation of reactive oxygen species (ROS) to eradicate bacteria in infectious microenvironments. Notably, CA@MSNs also demonstrated the ability to scavenge a broad spectrum of ROS, including hydroxyl free radicals, hydrogen peroxide, and superoxide radicals, in inflammatory microenvironments. This dual functionality helped mitigate inflammation and promote endothelial cell migration. Consequently, treatment with CA@MSNs significantly reduced inflammation, enhanced fibroblast activation, and facilitated collagen deposition, ultimately accelerating the healing of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds in diabetic mice. In conclusion, this study presents a promising therapeutic strategy for chronic diabetic wounds, offering a novel approach to overcoming infection-related healing delays.
miR-495 is a tumor-suppressor microRNA down-regulated in MLL -rearranged leukemia

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Acute myeloid leukemia (AML) is a heterogeneous group of hematopoietic malignancies with variable response to treatment. AMLs bearing MLL (mixed lineage leukemia) rearrangements are associated with intermediate or poor survival. MicroRNAs (miRNAs), a class of small noncoding RNAs, have been postulated to be important gene expression regulators virtually in all biological processes, including leukemogenesis. Through a large-scale, genome-wide miRNA expression profiling assay of 85 human AML and 15 normal control samples, we show that among 48 miRNAs that are significantly differentially expressed between MLL - and non– MLL -rearranged AML samples, only one (miR-495) is expressed at a lower level in MLL -rearranged AML than in non– MLL -rearranged AML; meanwhile, miR-495 is also significantly down-regulated in MLL -rearranged AML samples compared with normal control samples. Through in vitro colony-forming/replating assays and in vivo bone marrow transplantation studies, we show that forced expression of miR-495 significantly inhibits MLL-fusion-mediated cell transformation in vitro and leukemogenesis in vivo. In human leukemic cells carrying MLL rearrangements, ectopic expression of miR-495 greatly inhibits cell viability and increases cell apoptosis. Furthermore, our studies demonstrate that PBX3 and MEIS1 are two direct target genes of miR-495, and forced expression of either of them can reverse the effects of miR-495 overexpression on inhibiting cell viability and promoting apoptosis of human MLL -rearranged leukemic cells. Thus, our data indicate that miR-495 likely functions as a tumor suppressor in AML with MLL rearrangements by targeting essential leukemia-related genes.
Genetic Diversity of Pineapple (Ananas comosus) Germplasm in MalaysiaUsing Simple Sequence Repeat (SSR) Markers

Field: Agricultural and Biological Sciences (Life Sciences)

Assessments of genetic diversity have been claimed to be significantly efficient in utilising and managing resources of genetic for breeding programme. In this study, variations in genetic were observed in 65 pineapple accessions gathered from germplasm available at Malaysian Agriculture Research and Development Institute (MARDI) located in Pontian, Johor via 15 markers of simple sequence repeat (SSR). The results showed that 59 alleles appeared to range from 2.0 to 6.0 alleles with a mean of 3.9 alleles per locus, thus displaying polymorphism for all samples at a moderate level. Furthermore, the values of polymorphic information content (PIC) had been found to range between 0.104 (TsuAC035) and 0.697 (Acom_9.9), thus averaging at the value of 0.433. In addition, the expected and the observed heterozygosity of each locus seemed to vary within the ranges of 0.033 to 0.712, and from 0.033 to 0.885, along with the average values of 0.437 and 0.511, respectively. The population structure analysis via method of delta K (ΔK), along with mean of L (K) method, revealed that individuals from the germplasm could be divided into two major clusters based on genetics (K = 2), namely Group 1 and Group 2. As such, five accessions (Yankee, SRK Chalok, SCK Giant India, SC KEW5 India and SC1 Thailand) were clustered in Group 1, while the rest were clustered in Group 2. These outcomes were also supported by the dendrogram, which had been generated through the technique of unweighted pair group with arithmetic mean (UPGMA). These analyses appear to be helpful amongst breeders to maintain and to manage their collections of germplasm. Besides, the data gathered in this study can be useful for breeders to exploit the area of genetic diversity in estimating the level of heterosis.
Vortex dynamics on a pitching delta wing

Field: Engineering (Physical Sciences)

A study of the dynamic behavior of the leading-edge vortices on a delta wing undergoing oscillatory pitching motions is presented, A sharp-edged, flat-plate, delta wing having a sweep angle of 70 deg was used in this investigation. The wing was sinusoidally pitched about its one-half chord position at reduced frequencies ranging from /r = 2ir/c/i/ = 0.05 to 0.30 at root chord Reynolds numbers between 9xl04 and 3.5 x 10s, for angle-ofattack ranges of a = 29 to 39 deg and a = 0 to 45 deg. During these dynamic motions, visualization of the leadingedge vortices was obtained by injecting TiCl4 through ports located near the model apex. The location of vortex breakdown was recorded using high-speed motion-picture photography. The motion-picture records were analyzed to determine the vortex trajectory and breakdown position as a function of angle of attack. When the wing was sinusoidally pitched, hysteresis was observed in the location of the breakdown position. This hysteresis increased with reduced frequency. The velocity of breakdown propagation along the wing and the phase-lag between model motion and breakdown location were also determined. Detailed information was also obtained on the oscillation of breakdown position in both static and dynamic cases.
Correlation of morphological parameters with likelihood of thrombus formation in intracranial aneurysms

Field: Medicine (Health Sciences)

Accurate rupture prediction of intracranial aneurysms is crucial because not all aneurysms rupture.With past reports that aspect ratio (AR) and size ratio (SR) have significant correlations with rupture probability, these two morphological parameters are regarded as promising for rupture prediction: an aneurysm with a higher AR or SR is more likely to rupture than one with a lower AR or SR.However, 100% reliability of prediction is not guaranteed even with these parameters.It is necessary to clarify reasons for the high correlations of AR and SR with rupture status and what is responsible for unsuccessful predictions.In this paper, the authors discuss haemodynamic significance of the two parameters with a special focus on stagnant flow and resulting thrombus formation, which are considered to play important roles in the process of aneurysmal rupture.Results with computational fluid dynamics and a platelet aggregation model are also shown in order to demonstrate that stagnant flow and thrombus formation can be observed even in an aneurysm with low AR and SR.
A feminist critical discourse analysis of Ghanaian feminist blogs

Field: Social Sciences (Social Sciences)

Even though one of the aims of critical discourse analysis (CDA) is to demonstrate how social inequality, power abuse and discriminatory practices can be resisted, most studies have centered on the deconstruction of oppression and ideologically driven discrimination rather than the reconstruction of resistance. To fill this lacuna, the present paper examines the blogposts of Ghanaian feminists using feminist critical discourse analysis as an analytic framework. It analyzes resistance strategies utilized in the blogposts to criticize systematic gendering of privilege and inequality, and to foreground the voice and agency of Ghanaian/African women. Three resistance strategies were identified: (1) critiquing patriarchy, traditional gender norms and gender oppression, (2) resisting gender stereotypes and rewriting demeaning gender narratives, (3) calling out sexist attitudes and applauding women who resist such behavior. I argue that these strategies contribute to a feminist political critique of gendered social practices and relations aimed at effecting social emancipation and transformation. The paper ends by highlighting how the emancipatory discourse promoted by the blogs can be enhanced as part of a continuous striving for social justice for Ghanaian/African women.
Induced Ge spin polarization at the Fe/Ge interface

Field: Physics and Astronomy (Physical Sciences)

In this paper, we present direct evidence for induced spin on Ge at the Fe/Ge interface in an Fe/amorphous Ge multilayer. X-ray magnetic circular dichroism at the Ge L edge accesses the spin polarization of 4sp and 3d unoccupied density of states both showing antiparallel alignment to the adjacent Fe.
Dorsal GPi/GPe DBS Induced Dyskinesia (P1.8-037)

Field: Neuroscience (Life Sciences)

May 5, 2019April 9, 2019Free AccessDorsal GPi/GPe DBS Induced Dyskinesia (P1.8-037)Ahmad El Kouzi, Shannon Chiu, Matthew Burns, Robert Eisinger, Leonardo Almeida, Adolfo Ramirez Zamora, Kelly Foote, Michael Okun, and Wissam DeebAuthors Info & AffiliationsApril 9, 2019 issue92 (15_supplement)https://doi.org/10.1212/WNL.92.15_supplement.P1.8-037 Letters to the Editor