MP72-08 CELLULARIZED ELECTROSPUN VASCULAR GRAFTS FOR RENAL ARTERY RECONSTRUCTION

Field: Medicine (Health Sciences)

You have accessJournal of UrologyTransplantation & Vascular Surgery II1 Apr 2014MP72-08 CELLULARIZED ELECTROSPUN VASCULAR GRAFTS FOR RENAL ARTERY RECONSTRUCTION Hyunhee Ahn, Young Miin Ju, Cheil Kim, John D. Jackson, James J. Yoo, Sang Jin Lee, and Anthony Atala Hyunhee AhnHyunhee Ahn More articles by this author , Young Miin JuYoung Miin Ju More articles by this author , Cheil KimCheil Kim More articles by this author , John D. JacksonJohn D. Jackson More articles by this author , James J. YooJames J. Yoo More articles by this author , Sang Jin LeeSang Jin Lee More articles by this author , and Anthony AtalaAnthony Atala More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.2247AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Abnormal vascular conditions, such as renal artery stenosis due to fibrous dysplasia or aneurysms may require the use of vascular bypass grafts. However, the use of autologous vascular grafts in these patients is often limited by the availability and size mismatch. We previously have shown that an electrospun synthetic vascular scaffold is able to withstand physiological vascular conditions. In this study we examined whether fully cellularized vascular grafts could be created for use in renal artery reconstruction. METHODS Bilayered vascular scaffolds were electrospun using collagen type I mixed with poly(å-caprolactone) (PCL) in a 1:1 ratio. To demonstrate the applicability, autologous smooth muscle and endothelial cells were seeded on the exterior and luminal surface of the vascular scaffold, respectively, followed by preconditioning in a bioreactor system. Preconditioned cellularized scaffolds were implanted as an arterial interposition in the left carotid artery of sheep. Grafts without cells served as controls. The animals were evaluated at 4, 12, and 24 weeks after surgery using doppler ultrasound, CT angiography and histomorphological analyses. RESULTS The electrospun vascular grafts provided adequate surgical handling. Dual cell seeded scaffolds withstood physiologic hemodynamic conditions and the vessel constructs remained patent for 6 month in vivo. Grafts without cells resulted in stenosis and occlusion. The implanted grafts maintained a stable midgraft diameter and confirmed the absence of aneurysmal degeneration. The explanted grafts showed normal vascular tissue configuration without inflammation. CONCLUSIONS Dual cell seeded PCL/collagen electrospun grafts maintained a high degree of patency and structural integrity without eliciting a histologic inflammatory response over the course of a 6-month period in a sheep arterial interposition model. This study indicates that cellularized electrospun grafts may provide an alternative option for patients with renal artery stenosis. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e826 Advertisement Copyright & Permissions© 2014Metrics Author Information Hyunhee Ahn More articles by this author Young Miin Ju More articles by this author Cheil Kim More articles by this author John D. Jackson More articles by this author James J. Yoo More articles by this author Sang Jin Lee More articles by this author Anthony Atala More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...