Neotropicomus indicus Nanu & Kumar 2024, sp. nov.

Field: Agricultural and Biological Sciences (Life Sciences)

Neotropicomus indicus sp. nov. (Fig. 1) Neotropicomus indicus sp. nov. is characterized by basidiomata with a smooth to minutely pruinose, reddish brown pileus, stipe surface finely pruinose at the apex, downward to the middle with small reddish brown squamules, subfusoid to fusoid basidiospores, and a cutis type of stipitipellis interrupted by caulocystidia clusters. The species differs from N. australis by its smaller basidiospores, larger cheilocystidia and stipitipellis structure. TYPE MATERIAL. — India • Kerala State, Thiruvananthapuram District, Palode; 6.VII.2022; holotype: ZGC[ZGCSN180]; GenBank: OQ633210 (ITS), OQ633194 (28 S). ETYMOLOGY. — “ indicus ” indicates the distribution and collection locality of the species.GenBank accession numbersTaxonVoucherLocalityITS28SAustroboletus albidusYan C. Li & Zhu L. Yang HKAS107148, holotype China – NG088131A. olivaceoglutinosusK.Das & Dentinger HKAS57756 China – KF112383Boletus fagacicolaB.Feng, Y.Y.Cui, J.P.Xu & Zhu L.Yang HKAS55975, holotype China JN563899 JN563853B. reticuloceps(M.Zang, M.S.Yuan & M.Q.Gong) Q.B.Wang & Y.J. Yao HKAS57671 China – KF112454B. bainiuganDentinger HKAS52235 China JN563905 KF112457B. edulisBull. HMJAU4637 Russia JN563894 KF112455Brasilioporus olivaceoflavidusA.C.Magnago FLOR 68467 Brazil OM068901 –B. olivaceoflavidusFLOR 68468 Brazil OM068902 –B. olivaceoflavidusVIES 9901322, holotype Brazil OM068903 –B. rufonigricans(T.W. Henkel) A.C.Magnago & T.W.Henkel G472 Guyana KJ786658 –B. simoniarumA.C.Magnago VIES 9901327, holotype Brazil OM068905 OM068914Hortiboletus amygdalinusXue T. Zhu & Zhu L. Yang HKAS54166, holotype China KT990581 KT990777H. subpaludosus(W.F.Chiu) Xue T. Zhu & Zhu L. Yang HKAS59608 China KF112371 –Imleria badia(Fr.) Vizzini MB 03-098a United States – KF030355I. badiaXb2 Germany – KF030357I. obscurebrunnea(Hongo) Xue T. Zhu & Zhu L. Yang HKAS52557 China KC215207 KF112374Indoporus shoreaeA.Parihar, K.Das, Hembrom & Vizzini AP 6693, holotype India MK123972 MK123973I. shoreaeAP 6697 India MK123977 MK123976Neotropicomus australisA.C.Magnago FLOR 68471 Brazil OM068909 –N. australisACM1138 Brazil OM068907 OM068916N. australisVIES 9901328, holotype Brazil OM068906 –N. australisVIES 9901329 Brazil OM068908 OM068917N. indicussp. nov. ZGCSN180 IndiaOQ633210OQ633194N. parvogracilis(T.W.Henkel & Husbands) A.C.Magnago & T.W.Henkel TH9209, holotype Guyana JQ751261 JQ751262N. parvogracilisTH8850, paratype Guyana JQ751263 HQ161865Parvixerocomusaff.aokii(Hongo) G.Wu, N.K.Zeng & Zhu L. Yang HKAS52633 China – KF112379P. aokiiHKAS59812 China – KF112378P. pseudoaokiiG.Wu, K.Zhao & Zhu L. Yang HKAS80480, holotype China – KP658468Porphyrellus castaneusYan C. Li & Zhu L. Yang HKAS52554, holotype China – KT990697P. castaneusHKAS63076 China – KT990548P. orientifumosipesYan C. Li & Zhu L. Yang HKAS53372, holotype China – KT990629Strobilomyces echinocephalusGelardi & Vizzini HKAS59420 China – KF112463S. floccopus(Vahl) P. Karst. AFTOL-716 United States AY854068 AY684155S. floccopusAFTOL-716 United States AY854068 AY684155S. strobilaceus(Scop.) Berk. WU0016537 Austria – KT990647Tengioboletus glutinosusG.Wu & Zhu L. Yang HKAS53425, holotype China – KF112341T. glutinosusHKAS53452 China – KT990655T. reticulatusG.Wu & Zhu L. Yang HKAS52241 China – KT990657T. reticulatusHKAS53453, holotype China – KT990656Tylopilus dunensisA.C.Magnago & M.A.Neves MAN216, paratype Brazil MF113418 OM068920T. dunensisMAN218, holotype Brazil MF113419 MF113428T. felleus(Bull.) P. Karst. HKAS54926 Germany – KF112411T. felleusHKAS90203 China – KT990545T. violaceobrunneusYan C. Li & Zhu L. Yang HKAS89443, holotype China – KT990702Xanthoconium purpureumSnell & E.A.Dick NY00720964 United States – KT990663X. sinenseG.Wu, Y.Y.Cui & Zhu L. Yang HKAS77758, holotype China – KT990665Xerocomellus corneriXue T. Zhu & Zhu L. Yang HKAS52503 China – KT990668X. communisXue T. Zhu & Zhu L. Yang HKAS50467 China – KT990670Xerocomellussp. HKAS56311 China KF112340 KF112170 HABITAT. — Solitary, on ground in forest dominated by Dipterocarpaceae members. MYCOBANK. — MB848296. DESCRIPTION Basidiomata small-sized. Pileus 20-30 mm broad, convex when young, becoming plano-convex on maturity; surface viscid when wet, smooth to minutely pruinose, reddish brown to grayish brown towards the centre; margin straight. Pileal context whitish, unchanging when cut. Hymenphore adnate, depressed around the apex of stipe; surface pale yellow to olive yellow; pores 1-2 per mm, angular, unchanging on bruising; tubes 2-4 mm long, concolourous to the hymenophore surface, unchanging when cut. Stipe 20-40 × 3-4 mm, central, almost equal; surface finely pruinose at the apex downward to the middle with small reddish brown squamules, whitish to pale yellowish in apex, reddish brown in middle to bottom in a cream background. Stipe context whitish, unchanging on bruising. Basal mycelium white. Basidiospores 9-11× 4-5µm (Q=1.8-2.75, Qm= 2.2), subfusoid to fusoid, thin- to slightly thick-walled (up to 0.5µm), smooth, yellowish in water,inamyloid.Basidia 20-27×7-10µm, clavate, thin-walled, 4-spored, hyaline with many oil droplets; sterigmata up to 3 µm long, hyaline with many oil droplets. Pleurocystidia 42-62×6-12 µm, narrowly fusiform to fusiform, thin-walled, hyaline, inamyloid.Cheilocystidia 28-50×7-10 µm, narrowly utriform, nearly fusiform, broadly clavate or nearly cylindrical, hyaline, inamyloid. Hymenophoral trama phylloporoid; hyphae 3-15µm wide, thin-walled, hyaline.Pileipellis a trichodermium, made up of densely packed chains of terminal elements formed on subglobose cells; terminal elements 16-31×4-13 µm, nearly cylindrical or broadly clavate, some of them having subacute apex, thin-walled, inamyloid. Pileus trama tightly interwoven; hyphae 3-12 µm wide, thin-walled, hyaline, inamyloid. Stipitipellis a cutis interrupted by caulocystidia clusters; caulocystidia 13-28× 4-7 narrowly clavate to clavate, rarely with subacute apex, thin-walled, inamyloid. Stipe trama parallel to interwoven; hyphae 3-12 µm wide, thin-walled. Clamp-connections absent.