jimnoneill/paper-to-field-training huggingface dataset:
Abstract 134: ZEB2 as a therapeutic target in fusion-positive rhabdomyosarcoma

Field: Medicine (Health Sciences)

Abstract Rhabdomyosarcoma (RMS) is a highly aggressive childhood soft tissue cancer with skeletal muscle histogenesis. It is classified at the molecular level into fusion-negative (FN) and fusion-positive (FP) subtypes, with FP subtypes being defined by their signature oncofusions between the transcription factors PAX3 (or PAX7) and a variety of 3’ partners. Patients with FP-RMS have a five-year survival of &lt30%, and current treatments remain ineffective against the driving oncofusions. To address this challenge, we are interested in identifying proteins that interact with the oncofusions as alternate therapeutic targets, and conducted an unbiased proximity labeling screen for seven of the known RMS oncofusions. This approach revealed that ZEB2 is a common member of the interactome for all seven FP-RMS subtypes. ZEB2 is a DNA-binding transcriptional repressor that interacts with SMAD proteins and is known to be important for coordinating muscle and nerve development during embryogenesis. ZEB2 has been identified as an oncogene in various cancer types such as leukemia, glioblastoma, lung, and breast cancers. Further analysis of ZEB2's expression in FP-RMS cell lines, compared to skeletal muscle and other cancer cell lines, showed significantly higher levels of expression. In silico analyses of genomic CRISPR and RNAi screens in DepMap suggested a strong dependency of FP-RMS on ZEB2, with ZEB2 being one of the top four differential CRISPR dependencies in RMS cell lines compared to all other cancer cell lines. To investigate the functional consequences of ZEB2 suppression, we employed a loss-of-function approach using RNAi (siRNA and shRNA) in human FP-RMS cells. Our experiments confirmed the knockdown of ZEB2 through qRT-PCR and immunoblotting. Interestingly, the extent of ZEB2 knockdown determined whether FP-RMS cells underwent apoptosis or differentiation, potentially towards a neurodifferentiation pathway. We hypothesize that ZEB2 holds potential as a therapeutic target for FP-RMS patients. We aim to elucidate ZEB2's phenotypic role and mechanism of action in FP-RMS tumorigenesis, and assess its value as a therapeutic target in vivo. Our long-term goals for this project are to contribute to the development of innovative therapies that can mitigate the oncogenic activity driven by oncofusions in FP-RMS. Citation Format: Assil Fahs, Aanandi Munshi, Leen Barbar, Seth Zimmerman, Chris Counter, Corinne Linardic. ZEB2 as a therapeutic target in fusion-positive rhabdomyosarcoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 134.
USING THE EXPERIENCE OF TRAINING MILITARY PERSONNEL IN THE UNITED STATES TO ADVANCED TRAINING OF OFFICERS OF THE ARMED FORCES OF UKRAINE AND OTHER MILITARY FORMATIONS

Field: Social Sciences (Social Sciences)

The article deals with the problem of modernization of the training system for officers training of the Armed Forces of Ukraine and other military formations in the context of the modern strategic tasks of the development of the Armed Forces of Ukraine. The peculiarities of modern training of servicemen in the USA are highlighted and possible aspects of the use of this experience in the system of professional development of officers of the Armed Forces of Ukraine and other military formations. It is revealed that the existing system of training of servicemen in Ukraine does not fully meet the modern requirements and requires comprehensive modernization, which requires the study and implementation of advanced foreign experience in the training of officer personnel. The advantages of the military education system of the USA as one of the most powerful systems of military personnel training in the modern world are determined: the branch network of educational institutions providing training and advanced training of officer personnel at different stages of training according to the types of troops; systematic, phased and multi-level training, and advanced training; efficiency and dynamism; a combination of theoretical and practical training, civil and military education, teaching and research work; prestige, competitiveness and high efficiency. The urgent tasks of modernization of higher military education are defined taking into account the US experience: the transition to a professional army, introduction of modern standards of military activity, increase of flexibility and diversity of military education, increase of dependence of career growth on the level of qualification of servicemen, improvement of the structure of training of troops, attraction to teaching highly skilled , experienced military specialists, trainers (instructors) on highly specialized subjects, strengthening of scientific about maintenance of educational process, introduction of modern information technologies
Out-Patient Treatment with Faradic (Non-Convulsive Electric) Stimulation

Field: Medicine (Health Sciences)

Meeting Reports/Abstracts| June 26 2009 Out-Patient Treatment with Faradic (Non-Convulsive Electric) Stimulation Subject Area: Neurology and Neuroscience , Surgery Nathaniel J. Berkwitz Nathaniel J. Berkwitz Minneapolis, Minn. Search for other works by this author on: This Site PubMed Google Scholar Confinia Neurologica (1952) 12 (5-6): 362–363. https://doi.org/10.1159/000105803 Article history Published Online: June 26 2009 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Nathaniel J. Berkwitz; Out-Patient Treatment with Faradic (Non-Convulsive Electric) Stimulation. Confinia Neurologica 31 December 1952; 12 (5-6): 362–363. https://doi.org/10.1159/000105803 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsConfinia Neurologica Search Advanced Search Article PDF first page preview Close Modal This content is only available via PDF. 1952Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. You do not currently have access to this content.
Connecting Vulnerable Children and Families to Community-Based Programs Strengthens Parents' Perceptions of Protective Factors

Field: Health Professions (Health Sciences)

We employed principles from a nationally recognized prevention model on family support to investigate whether connecting vulnerable children to community-based programs and services through a statewide intervention system, the Help Me Grow program, strengthens parents' perceptions of protective factors. We used a parent survey modeled on 5 protective factors and related theoretical underpinnings of the Strengthening Families Protective Factors Framework to assess the impact of Help Me Grow on parents' perceptions of family circumstances and children's development. In addition, we coded and analyzed case notes completed by care coordinators to examine strategies for promoting protective factors. Parents reported a positive change in their family circumstances and a strengthening of protective factors. Parents' responses were positive despite differences in presenting issues. Help Me Grow support to families and their connection to programs and services enhanced parents' perceptions of protective factors even among families with differing needs. Our analyses support the practical utility of the Strengthening Families approach as a tool for engaging parents and assessing parents' perceptions of the effectiveness of interventions. A positive shift in parents' attitudes, knowledge, and behaviors contributes to engaged, supported, and educated parents who are better equipped to meet their children's needs and foster healthy developmental outcomes.
‘We need “culture-bridges”: professionals’ experiences of sex education for pupils with intellectual disabilities in a multicultural society

Field: Social Sciences (Social Sciences)

This study aimed to explore how sex education in special schools in Sweden is influenced and challenged by the multicultural aspects of modern society. In particular, it sought to explore professionalls' experiences of sex education and of honour-related experiences among young people with intellectual disabilities. Data from five individual interviews and one focus groups with four professionals were thematically analysed using sexual scripts as a theoretical framework. The results reveal an ambivalent honour-related script geared toward pupils with intellectual disabilities from cultural backgrounds differing from those of the Swedish mainstream. The provision of sex education, including information about honour-related experiences, is especially important because of these young people’s vulnerability; however, addressing the subject effectively is sensitive and complicated. Colleagues with different cultural backgrounds can act as ‘culture bridges’ for professionals who lack strategies, methods and materials. Increasing professionals’ prerequisite qualifications (e.g. further education, supervision) and adopting autonomy-promoted conduct can empower pupils with intellectual disability to exercise autonomy over their sexuality outside their immediate cultural context.
Full methylation of H3K27 by PRC2 is dispensable for initial embryoid body formation but required to maintain differentiated cell identity

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

ABSTRACT Polycomb repressive complex 2 (PRC2) catalyzes methylation of histone H3 on lysine 27 and is required for normal development of complex eukaryotes. The nature of that requirement is not clear. H3K27me3 is associated with repressed genes, but the modification is not sufficient to induce repression and, in some instances, is not required. We blocked full methylation of H3K27 with both a small molecule inhibitor, GSK343, and by introducing a point mutation into EZH2, the catalytic subunit of PRC2, in the mouse CJ7 cell line. Cells with substantively decreased H3K27 methylation differentiate into embryoid bodies, which contrasts with EZH2 null cells. PRC2 targets had varied requirements for H3K27me3, with a subset that maintained normal levels of repression in the absence of methylation. The primary cellular phenotype of blocked H3K27 methylation was an inability of altered cells to maintain a differentiated state when challenged. This phenotype was determined by H3K27 methylation in embryonic stem cells through the first 4 days of differentiation. Full H3K27 methylation therefore was not necessary for formation of differentiated cell states during embryoid body formation but was required to maintain a stable differentiated state.
Remember your brothers

Field: Arts and Humanities (Social Sciences)

Since the declaration of the Caliphate in 2014, the Islamic State became a prominent protagonism of contemporary political debate, especially for its unprecedented media flow. Moving from an already vast literature on the topic, the chapter will analyze the video-testaments produced by IS as an internal propaganda format, able to build a sense of community between the fighters and to inspire new generations to join the jihadi effort. After connecting this visual practice with its immediate predecessor (al-Qaeda martyrs' video-testaments), the chapter will provide an in-depth analysis of these videos, stressing the role of memory and inspiration, as well as their ability to produce a certain narrative of what it means to be a true Muslim.
From Protein Corona to Colloidal Self-Assembly: The Importance of Protein Size in Protein–Nanoparticle Interactions

Field: Chemistry (Physical Sciences)

Protein adsorption on nanoparticles is an important field of study, particularly with regard to nanomedicine and nanotoxicology. Many factors can influence the composition and structure of the layer(s) of adsorbed proteins, the so-called protein corona. However, the role of protein size has not been specifically investigated, although some evidence has indicated its potential important role in corona composition and structure. To assess the role of protein size, we studied the interactions of hemoproteins (spanning a large size range) with monodisperse silica nanoparticles. We combined various techniques-adsorption isotherms, isothermal titration calorimetry, circular dichroism, and transmission electron cryomicroscopy-to address this issue. Overall, the results show that small proteins behaved as typical model proteins, forming homogeneous monolayers on the nanoparticle surface (protein corona). Their adsorption is purely enthalpy-driven, with subtle structural changes. In contrast, large proteins interact with nanoparticles via entropy-driven mechanisms. Their structure is completely preserved during adsorption, and any given protein can directly bind to several nanoparticles, forming bridges in these newly formed protein-nanoparticle assemblies. Protein size is clearly an overlooked factor that should be integrated into proteomics and toxicological studies.
Incidence of symptomatic thromboembolic disease after patellofemoral arthroplasty.

Field: Medicine (Health Sciences)

Venous thromboembolic (VTE) disease is a risk after patellofemoral arthroplasty (PFA) despite its less invasive nature and faster recovery. To our knowledge, this is the first study to address the incidence of VTE disease after PFA. One hundred forty-nine consecutive primary or revision PFA procedures were followed for the primary endpoint of symptomatic VTE/pulmonary embolus within 6 weeks after surgery. VTE prophylaxis type, American Society of Anesthesiologists (ASA) class, VTE risk factors, and complications were noted. The mean age of the patient population was 48 years. Eighty-three percent of patients received aspirin and calf pumps; 17% received warfarin. The average operative time was 76 minutes overall and 66 minutes for isolated PFA. One patient with an undisclosed family history of VTE, who was managed with aspirin and calf pumps, developed multiple small pulmonary emboli. No other VTE complications occurred. Symptomatic VTE incidence was 0.76%. Patients without significant risk of thrombosis undergoing PFA may be effectively managed with an aspirin-based protocol.
Assessment of the impact of increased solar ultraviolet radiation upon marine ecosystems

Field: Environmental Science (Physical Sciences)

Data was provided to assess the potential impact upon marine ecosystems if space shuttle operations contribute to a reduction of the stratospheric ozone layer. The potential for irreversible damage to the productivity, structure and/or functioning of a model estuarine ecosystem by increased UV-B radiation was established. The sensitivity of key community components (the primary producers) to increased UV-B radiation was delineated.
Better dermatologic office photography: taking the photograph.

Field: Health Professions (Health Sciences)

In a previous article we reviewed the questions every medical photographer must ask when looking through the viewfinder, as well as the difference between plastic surgical and dermatologic photography. We conclude by offering a mnemonic for the three views necessary in dermatologic photography: Location, Scan, and Detail, and by suggesting a technique for preadjusting the camera, lens setting, and flash that will guarantee consistent, high-quality clinical photos.
Effect of wheat inclusion and xylanase supplementation of the diet on intestinal enzyme activity, nutrient retention and performance in laying hen from 25 to 47 wks of age

Field: Agricultural and Biological Sciences (Life Sciences)

A trial was conducted to examine the effects of increasing levels of wheat in the diet and xylanase (ES) supplementation on nitrogen and ether extract retention, pH of the GIT, productive performance from 25 to 47 wks of age, and enzyme activity at the small intestine level. The basal diets (from 25 to 33 wks and from 33 to 47 wks) consisted of soybean meal and corn, and the wheat was introduced in the experimental diets at expenses of corn, primarily.
Mass Media Trends: Implementation of AI, ML, SEO and Data Journalism in the Worldwide Media Landscape

Field: Social Sciences (Social Sciences)

This article refers to the use and implementation of new technologies, like AI (Artificial Intelligence), ML (Machine Learning), Big Data, data journalism and SEO optimization in traditional media, new media and brand journalism. The author explains what is “data journalism” in the opinion of Russian and English-speaking researchers. The article describes the main accidents and dates of the establishing of date journalism in the USA, Russia. The author examines how artificial intelligence and machine learning are implemented by editorial offices, as an example author takes “Forbes”, “Reuters”, “The Guardian” and other media outlets. This study is based on both primary and secondary sources of qualitative data to understand the challenges and opportunities to work in the ever-changing media landscape. Based on the empirical base, the author examines the implementation of data journalism in Latvia – using the materials published in collaboration with the author in the print magazine “Forbes Latvija” during the period of 2017– 2019. Based on research methods and the use of SEO tools while writing articles in English for an IT company in the period from 2019–2021, the author examines the impact of SEO promotion in traditional media, new media and brand journalism. There is a tendency nowadays that brand journalism is affecting the agenda of media, and having considered this issue in more detail, the author gives examples of specific SEO optimization and promotion tools that help brand journalism in achieving this goal.
A comparison of prednisolone enemas with low-dose oral prednisolone in the treatment of acute distal ulcerative colitis

Field: Medicine (Health Sciences)

The therapeutic efficacy of prednisolone metasulphobenzoate enemas in the treatment of distal colitis has been compared with that of low-dose oral prednisolone, in a single-blind, cross-over trial. Treatment with steroid enemas resulted in symptomatic and sigmoidoscopic evidence of improvement more frequently than treatment with a dosage of oral steroid calculated to achieve similar plasma prednisolone levels. In addition to demonstrating, for the first time, that prednisolone metasulphobenzoate enemas are effective in the treatment of colitis, this study suggests that their efficacy is due to a local action rather than systemic absorption.
Spontaneous curvature in two-dimensional van der Waals heterostructures

Field: Materials Science (Physical Sciences)

Two-dimensional (2D) van der Waals (vdW) heterostructures consist of different 2D crystals with diverse properties, constituting the cornerstone of the new generation of 2D electronic devices. Yet interfaces in heterostructures inevitably break bulk symmetry and structural continuity, resulting in delicate atomic rearrangements and novel electronic structures. In this paper, we predict that 2D interfaces undergo spontaneous curvature, which means when two flat 2D layers approach each other, they inevitably experience out-of-plane curvature. Based on deep-learning-assisted large-scale molecular dynamics simulations, we observed significant out-of-plane displacements up to 3.8 angstrom in graphene/BN bilayers induced by curvature, producing a stable hexagonal moire pattern, which agrees well with experimentally observations. Additionally, the out-of-plane flexibility of 2D crystals enables the propagation of curvature throughout the system, thereby influencing the mechanical properties of the heterostructure. These findings offer fundamental insights into the atomic structure in 2D vdW heterostructures and pave the way for their applications in devices.
FLOATING MULTIPARTICULATE SYSTEMS: A NOVEL APPROACH IN GASTRORETENTIVE DRUG DELIVERY SYSTEMS

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Recent advances in novel drug delivery system to enhance the safety and efficacy of the drug molecule by formulating a dosage form being convenient for administration. A controlled drug delivery system with prolonged residence time in the stomach can be of great practical importance for drugs with an absorption window in the upper small intestine. The main limitations are attributed to the inter- and intra-subject variability of gastro-intestinal (GI) transit time and the non-uniformity of drug absorption throughout the alimentary canal. Floating drug delivery systems (FDDSs) are expected to remain buoyant in a lasting way upon the gastric contents and consequently to enhance the bioavailability of drugs. The various buoyant preparations include hollow microspheres, granules, beads and powders. Multiparticulate low-density particles can successfully prolong the gastric retention time of drugs. This article is a review of important approaches utilized in the preparation of floating multiparticulate systems, characterization of floating multiparticulate systems and recent research work done on floating multiparticulate systems.