GB Virus C Infection and Liver Transplantation: Increased Risk of Transfusion-Transmitted Infection

Field: Medicine (Health Sciences)

taining regimens).Residual positivity was observed in 52% (13 of Re ´gis Costello Danie `le Sainty 25) of samples taken within 8 months after diagnosis.Of the 6 patients analyzed before the second month, 5 showed strong PCR Didier Blaise Jean-Albert Gastaut positivity, whereas only 1 patient was negative.This early RT-PCR positivity did not predict for early relapse.Molecular conversion to Jean Gabert Department of Hematology RT-PCR negativity within 8 months was observed in 6 patients, none of whom have relapsed, with a median follow-up of 18 months Institut Paoli-Calmettes Marseilles, France (range, 7 to 24 months), although 1 patient (UPN 9) died at 7 months after diagnosis from graft-versus-host disease.In contrast, 3 of the He ´le `ne Poirel Agne `s Buzyn-Veil 4 patients remaining positive by 8 months have relapsed at 16, 22, and 8 months.We conclude that, in contrast to early (õ2 months)Elizabeth Macintyre Ho ˆpital Necker-Enfants Malades PCR positivity, absence of PCR conversion within the first 8 months seems to be predictive for hematologic relapse and could represent Paris, France an indication for more intensive therapy, if these data are confirmed in a larger series.The superior predictive value of CBFb/MYH11 REFERENCES detection in AML in comparison with BCR/ABL in CML could be 1.Poirel H, Radford-Weiss I, Rack K, Troussard X, Veil A, explained by its lower sensitivity, in keeping with results obtained for Valensi F, Picard F, Guesnu M, Leboeuf D, Melle J, Dreyfus F, PML/RARa detection in acute promyelocytic leukemia 7 and ALL-1/ Flandrin G, Macintyre E: Detection of the chromosome 16 CBF AF-4 in acute lymphoblastic leukemia. 8b-MYH11 fusion transcript in myelomonocytic leukemias.Blood We also report the detection of CBFb/MYH11 fusion transcripts 85:1313, 1995 in cytapheresis products in four patients who underwent peripheral 2. Hebert J, Cayuela JM, Daniel MT, Berger R, Sigaux F: Detecblood stem cell (PBSC) mobilization using granulocyte colonytion of minimal residual disease in acute myelomonocytic leukemia stimulating factor (G-CSF ) after the third course of chemotherapy.with abnormal marrow eosinophils by nested polymerase chain reac-Three patients were RT-PCR negative in the cytapheresis products tion with allele specific amplification.Blood 84:2291, 1994 and are still in hematologic and molecular complete remission at 3. Tobal K, Johnson PR, Saunders MJ, Harrison CJ, Liu Yin JA: 18 to 24 months from diagnosis, whereas the patient who showed Detection of CBFb/MYH11 transcripts in patients with inversion clear RT-PCR positivity in the cytapheresis products experienced and other abnormalities of chromosome 16 at presentation and remisearly relapse.These data correlate with PCR results obtained in sion.Br J Haematol 91:104, 1995 peripheral blood independently from G-CSF PBSC mobilization, 4. Claxton DF, Liu P, Hsu HB, Marlton P, Hester J, Collins showing that growth factor use does not seem to recruit leukemic F, Deisseroth AB, Rowley JD, Siciliano MJ: Detection of fusion cell progenitors despite the in vitro proliferative effect on AML transcripts generated by the inversion 16 chromosome in acute myblasts.Moreover, these data suggest that RT-PCR analysis of elogenous leukemia.Blood 83:1750, 1994 CBFb/MYH11 transcripts could be used to objectively assess blas-5.Liu PP, Hajra A, Wijmenga C, Collins FS: Molecular pathotic contamination and the efficacy of purging treatment in cytaphergenesis of the chromosome 16 inversion in the M4eo subtype of esis products.acute myeloid leukemia.Blood 85:2289, 1995 Large prospective series and longer follow-up are required to more 6.Costello R, Lafage M, Toiron Y, Brunel V, Sainty D, Arnoulet accurately determine the value of MRD detection by RT-PCR, using C, Mozziconacci M, Bouabdallah R, Gastaut J, Maraninchi D, Gabert CBFb-MYH11 fusion transcripts as the molecular marker, to refine J: Philadelphia chromosome-negative chronic myeloid leukemia: A prognosis and modulate postremission treatment modalities in accorreport of 14 new cases.