jimnoneill/paper-to-field-training huggingface dataset:
Myocytes and fibroblasts exhibit functional synergism in mixed cultures of neonatal rat heart cells

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Abstract Cardiac cells obtained from neonatal rat heart contain a mixed population of cell types that can be enriched in culture in either myocytes or fibroblast‐like cells. A metabolic comparison of mixed heart cell cultures with enriched cultures of the same age‐in‐culture and initial cell density showed that mixed cultures used glucose more rapidly than either enriched myocytes or fibroblasts. Mixed cultures were shown to respond to deprivation of insulin and of serum with decreases in the rate of glucose usage and decreases in the protein content of cells, whereas enriched cultures did not respond in the expected manner to insulin deprivation. Mixed, 11‐day‐old cells also exhibited greater increases in cellular protein and greater resistance to the stress of starvation than enriched cultures. Palmitate usage, however, was similar in all cultures examined. We conclude that mixed cultures may serve as a better model system to study cardiac metabolism and to monitor the effects of drugs and hormones on the neonatal myocardium. In addition, it is clear from our results that myocytes and fibroblastic‐like cells coexist in a metabolically functional synergism.
Abstract 362: Role of Dysregulated Exosomal MiRNAs in Functional Impairment of Cardiac Endothelial Cells

Field: Medicine (Health Sciences)

Objective: Endothelial cells (ECs) play critical role to maintain the normal heart function. It is shown that fibroblast-derived exosomes have the ability to enhance cardiac myocytes hypertrophy in pressure-overloaded myocardium. However, their effect on endothelial cell function has not been studied. Others and we have previously shown that stress-induced chronic inflammation induces cardiac fibroblasts and mediates endothelial cells dysfunction. Here we hypothesized that activated cardiac fibroblasts-derived exosomes (FB-Exo) mediates cardiac ECs dysfunction and leads for cardiac pathology and restoring the altered FB-Exo contents will improve endothelial cells function and biology. Methods: We cultured mouse primary endothelial cells in EC growth media. Cells were treated with fibroblasts-derived-Exo. Exosomes were isolated from fibroblast condition media by ultracentrifugation and characterized by nanosight & electron microscopy. Results: Fibroblasts were significantly activated by TGFβ treatment as shown by qPCR and western blot (smad2/3, p-Smad2/3, p38, p-p38) data. Endothelial cells dysfunction as shown by Matrigel assay, real-time Q-PCR and western data (eNOS and Hif1α) data was observed in TGFβ-FB-Exo treatment endothelial cell. MTT, TUNEL and migration assay also followed the same trend as TGFβ-FB-Exo treatment significantly induced endothelial cell death and inhibits its proliferation and migration. Furthermore, microRNA array and PCR analysis revealed dysregulation of miR-132-3p, miR-2001-3p and miR-125b-5p in TGFβ-FB-Exo treated endothelial cells. Conclusions: Taken together, this study demonstrates that TGFβ treated fibroblasts-derived exosomes are enriched in pro-fibrotic factors and can lead to endothelial dysfunction and promotes cardiac fibrosis in PO myocardium. In future study, we will modulate the target miRs in fibroblasts to see whether it rescue reparative function of endothelial cell and inhibits cardiac fibrosis in failing heart.
MicroRNA expression profiling outperforms mRNA expression profiling in formalin-fixed paraffin-embedded tissues.

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

microRNAs (miRNAs) are approximately 22nt RNAs that regulate target gene expression. Altered expression of miRNAs has been demonstrated in many different human cancers. Many studies using microarray technologies to characterize miRNA expression profiles have relied on fresh tissue to determine the miRNA signatures. In this study, we prepared total RNA from paired samples of formalin-fixed paraffin-embedded (FFPE) and fresh frozen malignant melanoma, and used that in microarray experiments to compare miRNA expression profiles between FFPE and fresh tissue with corresponding mRNA expression profiles from the same tissue sources. We demonstrate that miRNA expression profile from FFPE tissues closely resembles that from fresh tissues, and the correlation is significantly better than that for mRNA profiles from FFPE and fresh tissues. These results underscore the suitability of FFPE tissues as appropriate resources for molecular expression analyses and support the notion that miRNAs are more vigorous analytes for this purpose than mRNAs.
[Parameters of the elimination of phenolsulfonphthaleine (phenolred) in children (author's transl)].

Field: Medicine (Health Sciences)

The elimination of phenol-red was examined in 124 children without renal diseases. The normal values of the kinetic parameters were determined after intravenous injection of 3-4 mg dye per kg bodyweight. The elimination-half-life, which is independent from bodyweight and length was used as measure for the renal tubular secretion. In newborns (85,8 min) and young infants up to the age of 6 months (25,4 min) elimination-half-lives were longer than in older ones. After the second year of life (19,7 min) normal values of adults could be determined. The total clearance of phenol-red is smaller in newborns (185 ml/kg/h) than in children older than one year (643 ml/kg/h). Prematures had a longer elimination-half-life and a smaller total clearance than mature newborns.
Queen Willy

Field: Arts and Humanities (Social Sciences)

This video was made for The San Antonio Public Access Tv Show. The Springtime65 Show Saturdays at 10:30pm Time Warner Digital Channel 20 and 19 Grande. www.myspace.com/boygeorgeroland. Filmed inside Atlantis Discoverd jewerly Provided by Atlantis Disovered. 7038 Bandera Rd San Antonio TX 78238 . 210-523-7900 atlantis-discovered.com Roland De Leon Exective producer The Springtime65 Show A Springtime65 Production
Applications of Percutaneous Nephrostomy. New Challenges and Opportunities in Endo-Urology

Field: Medicine (Health Sciences)

No AccessJournal of UrologyLetter to the Editor1 Mar 1979Applications of Percutaneous Nephrostomy. New Challenges and Opportunities in Endo-Urology Arthur D. Smith, Paul H. Lange, and Elwin E. Fraley Arthur D. SmithArthur D. Smith More articles by this author , Paul H. LangePaul H. Lange More articles by this author , and Elwin E. FraleyElwin E. Fraley More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)56797-XAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Applications of Percutaneous Nephrostomy. New Challenges and Opportunities in Endo-Urology." The Journal of Urology, 121(3), p. 382 © 1979 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byFritzsche P, Moorhead J, Axford P and Torrey R (2018) Urologic Applications of Angiographic Guide Wire and Catheter TechniquesJournal of Urology, VOL. 125, NO. 6, (774-780), Online publication date: 1-Jun-1981. Volume 121Issue 3March 1979Page: 382 Advertisement Copyright & Permissions© 1979 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Arthur D. Smith More articles by this author Paul H. Lange More articles by this author Elwin E. Fraley More articles by this author Expand All Advertisement PDF downloadLoading ...
The direction of the teaching learning process in the annexed classroom of mechanics of Self-driven Vehicles, some considerations for its development

Field: Social Sciences (Social Sciences)

The formation of the qualified work force is of vital importance for the integral development of the country, starting from the generalization of experiences that in different contexts one comes developing in the Technical Education and Professional. The present article points out the theoretical budgets that should be kept in mind for such an aspiration, making emphasis in the direction of the process of teaching learning in the specialty of Mechanics of Self-driven Vehicles in the annexed classrooms of the Labor Entities.
Correlation Between Attending Physicians’ Empathy and Readmission Rate in Internal Medicine

Field: Medicine (Health Sciences)

Introduction: Physician empathy has been correlated with several outcomes in outpatient settings, demonstrating better prognosis in patients followed by highly empathetic doctors. Aim: To correlate empathy of Internal Medicine ward physicians with readmission rates of patients they took care of. Methods: We extracted readmission data of all patients discharged from Internal Medicine wards at our hospital between January 1st, 2011, and May 31st, 2017. Patients discharged to other institutions (nursing homes, rehabilitation units, other hospitals) were excluded. The 30-day readmission rate was recorded for each doctor. We measured the empathy of physicians who discharged more than 100 patients during that period, using the Jefferson Scale of Empathy (JSE) and the Empathy Components Questionnaire (ECQ). Correlation between empathy scores and readmission rate was weighted for the number of patients followed by each physician and adjusted for sex, age and relative cost weight of patients, as well as the doctor’s age. The same correlation was calculated in the subgroup of index patients discharged with Diagnosis Related Group 127 (DRG 127, Heart Failure and Shock). Results: A total of 4280 index discharge events were identified, 383 of which (8.9%) were readmitted within 30 days. JSE scores were found out to be inversely correlated with readmission rates (coefficient -0.027, R2 0.181, p<0.001) whereas ECQ correlation was not significant after adjustment. A significant inverse correlation was also observed considering only the DRG 127 patients, with both Empathy scales (JSE and ECQ, coefficient -0.032 and -0.098, R2 0.303 and 0.326, p=0.050 and p<0.001, respectively). Conclusion: Empathy of Internal Medicine physicians correlates with readmission rates of the patients they cared for, especially heart failure patients for whom this is a relevant outcome.
Evaluating the Impacts of Competition Policy Reforms on the Efficiency of Philippine Commercial Banks

Field: Economics, Econometrics and Finance (Social Sciences)

This paper has attempted to examine the impacts of competition policy reforms on the efficiency of the Philippine commercial banking system. It uses the stochastic frontier approach to come up with estimates of profit efficiency and cost inefficiency measures. The results are quite interesting. First, the average measured profit efficiency is 0.85, implying that on the average the commercial banks are using only 85 percent of their resources efficiently compared to the best practice commercial bank in the system producing the same output and facing the same conditions. On the other hand, the average measured cost inefficiency of the commercial banks is 1.39, suggesting that, on average, 39 percent of the commercial bankâ€™s costs are wasted relative to the best-practice commercial bank in the system producing the same output and facing the same conditions. Second, some improvements in banksâ€™ profit and cost efficiency can be observed after the liberalization of the entry of foreign banks in 1994, but these improvements were halted when the East Asian financial crisis occurred. Some improvements in profit and cost efficiency can again be observed after the passage of the General Banking Law in 2000 that liberalized further the entry of foreign banks. Third, small banks are found to be more profit and cost efficient than large banks. Fourth, foreign banks are generally more profit and cost efficient than domestic banks. However, these differences widen during crisis period and narrow during stable economic conditions. Fifth, profit efficiency of merged banks dropped more sharply than nonmerged banks after 1998, but eventually recovered and approximated that of nonmerged banks in 2002. Also, merged banksâ€™ cost inefficiency dropped sharply in 2000 and since then has remained much lower than that of nonmerged banks. Sixth, some factors, such as agency problem, governance and market characteristics appear to be significantly correlated with measured efficiencies of banks. These results have important policy implications. First, the liberalization of the banking system has generally produced positive results in terms of improving profit and cost efficiencies of banks. Second, improvement in profit and cost efficiencies of domestic banks brought about by greater competition cannot be sustained unless it is accompanied by improvement in prudential regulations and supervision. Third, M&A policy pursued by the Bangko Sentral ng Pilipinas appears to be complementary policy for improving profit and cost efficiencies of banks. Fourth, understanding the nature and extent of the impact of some correlates of measured efficiencies can help authorities in designing appropriate regulatory and supervisory framework for banks.
Comparing two synthesis methods: Exploring unique characteristics and catalytic activity of fenton catalyst Fe3O4/zeolite-NaY in methylene blue degradation

Field: Chemical Engineering (Physical Sciences)

A composite catalyst was developed to degrade methylene blue (MB) via the Fenton reaction from Fe3O4 and H2O2 with zeolite-NaY as magnetite support to reduce agglomeration. Hydrothermal and co-precipitation approaches were used to synthesize the composite. The co-precipitation method here was proven to be more suitable to produce the composite. Under optimal conditions, all composite catalysts achieved a remarkable MB reduction (more than 90 %) as confirmed through TLC analysis and UV–Vis spectra. Notably, based on linear regression, FeZ-A maintained over 60 % activity after 15 uses, while FeZ-2 showed the least Fe ion leaching. This demonstrated the effectiveness and stability of the developed catalysts.
CAMTA1 is a useful immunohistochemical marker for diagnosing epithelioid haemangioendothelioma

Field: Medicine (Health Sciences)

Aims The diagnosis of epithelioid haemangioendothelioma (EHE) is usually straightforward, based on characteristic histological features. However, it is sometimes difficult to differentiate EHE from a variety of other tumours with epithelioid morphology. The WW domain‐containing transcription regulator 1–calmodulin‐binding transcription activator 1 ( WWTR1–CAMTA1 ) fusion gene, resulting in the overexpression of CAMTA1, is demonstrated in approximately 90% of EHEs, and the yes‐associated protein 1–transcription factor E3 ( YAP1–TFE3 ) fusion gene, associated with the strong and diffuse nuclear expression of TFE3, is present in another small subset of EHEs. The aim of our study was to examine CAMTA1 expression in EHEs and a variety of other tumours to evaluate its diagnostic utility, and to analyse TFE3 expression status in EHEs. Methods and results Immunohistochemistry was performed on 16 EHEs, including five cases with CAMTA1 rearrangement and 276 non‐EHE tumours. Fourteen of 16 EHEs and only one case of ductal carcinoma of the breast were positive for CAMTA1 and its expression was focal and weak in the latter (sensitivity 87.5%, specificity 99.6%). TFE3 expression was expressed focally and weakly in three (19%) EHEs (two with the CAMTA1 rearrangement). Conclusions Nuclear CAMTA1 expression is sensitive and highly specific for EHE and can be applied to diagnostic immunohistochemistry in epithelioid tumours.
The Excluded Volume Problem in the Polymer Reference Interaction Site Model

Field: Chemistry (Physical Sciences)

An intramolecular distribution function for lattice chains, accounting for the long range excluded volume, has been derived employing an Ornstein-Zernike-like integral equation approach, initially investigated for off-lattice chains by Curro, Blatz, and Pings. The intramolecular distributions, obtained for 16-, 30-, and 60-mers, are compared to theoretical results for the freely jointed chain model and to Monte Carlo simulation data. The new single chain intramolecular distribution function accurately matches the Monte Carlo data whereas the ideal chain structure factor overestimates the short-range intramolecular correlations. Upon increasing density the simulated intramolecular distributions gradually evolve toward the ideal chain results although the latter are never actually reached. The intermolecular distributions for athermal and interacting 30-mers at several densities have been calculated by combining the investigated intramolecular distribution functions with the lattice-PRISM theory. Furthermore, the equation of state for athermal 20- and 30-mers and the liquid-vapor spinodal for 16-mers have been investigated. It is found that the ideal chain distribution function results in unphysical thermodynamic behavior, reflected in the equation of state as well as in the liquid-vapor spinodal. The predicted intermolecular distribution is quite reasonable, considering the simplicity of the ideal chain model. The combination lattice-PRISM/excluded volume intramolecular distribution function offers at least qualitatively correct results both for the thermodynamic properties and intermolecular correlations.
Peripheral nerve injury

Field: Medicine (Health Sciences)

Abstract In blast injury, the patient is exposed to a shock wave at close range, without any fracture, or signs of significant soft tissue injury. The underlying mechanism is, as yet, not fully understood. Large fibre conduction recovers, but the small fibres repair more slowly, and sometimes imperfectly. This chapter discusses how to assess and surgically address peripheral nerve injury. It is generally advocated that patients with traumatic peripheral nerve injuries, including closed brachial plexus traction lesions, should undergo surgery as early as possible, as this has been shown to optimise recovery. Peripheral nerve injuries offer many challenges, especially those that are proximal, involving the nerve plexus, and/or are complicated by other injuries. Secondary distal damage to a peripheral nerve can further confuse the clinical picture. A thorough history and examination, together with appropriate imaging and neurophysiological investigations, allow accurate diagnosis and prognosis. The management of such severe blast injury to peripheral nerves is a new phenomenon requiring further research.
A Multi-Purpose Planetary Gear Testing Machine for Studies of Gear Drive Dynamics, Efficiency, and Lubrication

Field: Engineering (Physical Sciences)

Feasibility of a multi-purpose testing machine for research studies in gearing has been demonstrated with construction of a unique gear testing machine with a differential planetary gear drive. This machine was used in such interdependent studies as determination of instantaneous gear tooth engagement loads, minimum film thicknesses, and gear efficiencies. With minimal structural and mechanical modifications, this gear research machine can be used for studies of surface durability, thermal distribution in gear meshing zones, and effects of variable torques and torsional oscillations on performance of gearing. Most of these studies could be conducted simultaneously. Upon selection of appropriate gear ratios, this machine was operated either with one or two stationary gears. Presence of stationary gears simplified greatly the measurement techniques and increased the reliability of tests. This machine can accommodate spur, helical or any special type of gearing. Design and operational characteristics of this machine, as well as a short summary of research projects performed on this machine, are presented in this paper.
Emerging technologies and distributed learning in higher education. Retrieved December 3, 2000, from the World Wide Web: http://www.virtual.gmu.edu/SS_research/cdpapers/hannapdf.htm

Field: Social Sciences (Social Sciences)

This chapter depicts visions of how sophisticated information technologies may influence the nature of higher education over the next couple decades. Its purpose is not to present predictions of an inevitable future, but instead to stimulate reflection about how we can collectively evolve colleges and universities by creatively moving beyond almost unconscious assumptions we hold about teaching, learning, and schooling. The rapid advance of information technology is driving shifts in every other form of societal institution, in ways that are often unplanned and sometimes unfortunate. Hopefully, the academy can do better in determining its own fate through deliberate choice and collaborative action to achieve the full educational potential of advanced computers and telecommunications. Emerging Interactive Media The development of the Internet is fostering the creation and proliferation of emerging interactive media, such as the WorldWide Web and shared virtual environments. A medium is in part a channel for conveying content; as the Internet increasingly pervades society, university instructors can readily reach extensive, remote
Antiviral effects of a synthetic Aluminium-Magnesium Silicate, on Avian Influenza Virus

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Effects a synthetic Aluminium-Magnesium Silicate [AMS] has on Avian Influenza Virus (AIV) were tested. Equal amounts of AIV samples and of the AMS were mixed, kept one hour at room temperature before centrifuging. The supernatants were remeasured and tested for, viral titre, Mean Death Time (MDT) and Mortality Rate of chicken Embryos (EMR). Volumes of the viral samples reduced at rate of 23.4% ± 5.48%. Viral titres reduced significantly (P < 0.01) from HA, 73 ± 32.72 to 1.4 ± 0.43. Also, EMR of infected chicken embryos reduced from 100% to 65%, while MDT of those that died,increased significantly (P < 0.01) from 76 ± 4.38 to 136 ±18.93 hours. When incubation with AMS was repeated on portions of an AIV sample, MDT increased from 64 to 104 hours with the portion incubated once. AIV portions on which incubation with AMS was repeated could not kill chicken embryos.