jimnoneill/paper-to-field-training huggingface dataset:
Anterior-enriched filopodia create appearance of asymmetric membrane microdomains in polarizing C. elegans zygotes

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

The association of molecules within membrane microdomains is critical for the intracellular organization of cells. During polarization of the C. elegans zygote, both polarity proteins and actomyosin regulators associate within dynamic membrane-associated foci. Recently, a novel class of asymmetric membrane-associated structures was described that appeared to be enriched in phosphatidylinositol 4,5-bisphosphate (PIP2), suggesting that PIP2 domains could constitute signaling hubs to promote cell polarization and actin nucleation. Here, we probe the nature of these domains using a variety of membrane- and actin cortex-associated probes. These data demonstrate that these domains are filopodia, which are stimulated transiently during polarity establishment and accumulate in the zygote anterior. The resulting membrane protrusions create local membrane topology that quantitatively accounts for observed local increases in the fluorescence signal of membrane-associated molecules, suggesting molecules are not selectively enriched in these domains relative to bulk membrane and that the PIP2 pool as revealed by PHPLCδ1 simply reflects plasma membrane localization. Given the ubiquity of 3D membrane structures in cells, including filopodia, microvilli and membrane folds, similar caveats are likely to apply to analysis of membrane-associated molecules in a broad range of systems.
Transcriptome - wide bioinformatics analysis of the binding sites of RNA - binding proteins and their putative role in mendelian diseases

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Background: Post-transcriptional regulation is the control of gene expression at the RNA level. After produced, the stability and distribution of the different transcripts are regulated by means of RNA-binding proteins (RBPs). Mutations in RNA-binding proteins can cause Mendelian diseases - prominently neuromuscular disorders and cancers. This study determines the interaction between RBPs and target-RNA complexes from public data of the ENCODE project and identifies mutations associated with Mendelian diseases that could disrupt the RBP-RNA interactions. Materials and methods: we performed a transcriptome - wide bioinformatics prediction of the binding sites of RBPs in the human transcriptome from public data of the ENCODE project. Results: The majority (54%) of pathogenic mutation putatively affecting the binding sites of RBPs are located in protein - coding genes and are mainly classified as loss - of - function mutations. Mutations located in the binding sites of RBPs related to RNA processing. For 13 diseases, Familial hypercholesterolemia is the most significant disease with about 40% of mutations in ClinVar database located into the binding sites of RBPs (p=2.3e-65), but congenital hypogonadotropic hypogonadism is the disease with the highest percentage of mutations affecting the binding sites of RBPs (98%, p=2.7e-25). The RBPs most involved in human Mendelian diseases by binding sites-disrupting mutations are YBX3, AQR and PRPF8. Conclusions: A large number of Mendelian diseases are potentially mediated by disease - causing variants that potentially disrupt the binding sites of RBPs. This will provide insight sharper on post - transcriptional mechanisms. Besides, it is useful to know the role of protein - RNA interactome networks in pathologies, thereby serving the treatment of diseases. Key words: bioinformatics analysis, ENCODE project, ClinVar, RNA-binding proteins, Mendelian diseases
NFVGuard: Verifying the Security of Multilevel Network Functions Virtualization (NFV) Stack

Field: Computer Science (Physical Sciences)

Network Functions Virtualization (NFV) enables agile and cost-effective deployment of multi-tenant network services on top of a cloud infrastructure. However, the multi-tenant and multilevel nature of NFV may lead to novel security challenges, such as stealthy attacks exploiting potential inconsistencies between different levels of the NFV stacks. Consequently, the security compliance of a multilevel NFV stack cannot be sufficiently established using existing solutions, which typically focus on one level. Moreover, the naive approach of separately verifying every level could be expensive or even infeasible. In this paper, we propose, NFVGuard, the first multilevel approach to the formal security verification of NFV stacks. Our key idea is to conduct the security verification at only one level, and then assure that verification result for other levels by verifying the consistency between adjacent levels. We integrate NFVGuard with OpenStack/Tacker, a popular platform for the NFV deployment, and experimentally evaluate its effectiveness.
Solubilization of Pyridone-Based Fluorescent Tag by Complexation in Cucurbit[7]uril

Field: Chemistry (Physical Sciences)

Aimed at further exploring the hosting properties of cucurbit[7]uril (CB7), we have exploited the spectroscopic and photophysical properties of a known fluorescent label as the guest molecule, namely, 3-cyano-6-(2-thienyl)-4-trifluoromethyl pyridine (TFP), in neat solvents. The formation of an inclusion host-guest complex with CB7 was checked by UV-vis absorption spectroscopy, and the value of binding constant (9.7 × 105 M-1) was extracted from the spectrophotometric data. The modulation of keto-enol equilibrium in TFP by the local environment is governed by the interplay between dimerization through intermolecular hydrogen bonding between individual solute molecules, favoring the enol form, and intermolecular hydrogen bonding between TFP and the surrounding solvents, favoring the keto form. Time-resolved fluorescence results established that the macromolecular CB7 host stabilizes preferentially the neutral enol form over the keto form of TFP. Unprecedentedly, our results reveal a linear dependence of the amplitudes of the extracted decay-associated spectra from the time-resolved fluorescence spectra of TFP on the sum of polarity/polarizability and hydrogen bonding parameters of the local environment, confirming that TFP at micromolar concentration in the CB7 complexes is experiencing a methanol-like environment. The results rationalized the 42-fold enhancement in the solubility of TFP in water media by complexation in CB7.
Face Recognition using Groups of Images in Smart Room Scenarios

Field: Computer Science (Physical Sciences)

In this paper, we present a technique for face recognition in smart environments. The technique takes advantage of the continuous monitoring of the scenario and combines the information of several images to perform the recognition. Appearance based face recognition techniques are used given that unobtrusive systems are required in this type of applications and that the scenario does not ensure high quality images. Models for the users are created on-the-fly and subsequent face images of the same individual are gathered into groups. Images within a group are jointly compared to the models for identification and verification purposes. Reliable face images are used to update the user's models. The proposed technique is assessed with the BANCA and CHIL databases.
Determination of berberine hydrochloride in sanhuangpian by HPLC

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Aim To esbablish a method for determining the berberine hydrochloride in sanhuangpian.Methods The C_(18) column was used(4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile-KH_2PO_4(adjusted to pH5.0 with sodium Hydroxide).The flow rate was 1.0 ml·min~-~1 with detection wavelength at 347 nm and column temperature at 30℃.Results The calibration curve was linear in the range 0.562～28.1 mg·L~-~1 of diosgenin with correlation coefficient 0.99998.The average recovery of diosgenin was 99.9%.Conclusion The method is simple,rapid,accurate and precise.
Aging and God: Spiritual Pathways to Mental Health in Midlife and Later Years

Field: Psychology (Social Sciences)

Contents Foreword * Preface * Part I. Religion and the Mental Sciences * The History of Psychiatry and Religion * Sigmund Freud * Conflicting Approaches to Mental Health * Overlap and Complementarity * Part II. Theoretical Issues * Theories of Human Development * Fowler's Stages of Faith Development * A Theory of Religious Faith Development * Part III. Advances in Research * Depression and Medical Illness * Using Religion to Cope * Cases of Religious Coping in Action * Religious Coping and Depression * Religion and Anxiety * Part IV. Clinical Applications * Spiritual Needs of Physically Ill Elders * Meeting Psychological and Spiritual Needs * Use of Religion in Psychotherapy * V. Special Concerns in Later Life * The Nursing Home and Alzheimer's Disease * Alcoholism/Sexual Dysfunction * The Family and Bereavement Issues * Religious Conversion * VI. The Final Frontier * Hope for the Dying * Suicide in Later Life * Physician-Assisted Suicide * Final Comments * Indexes * Reference Notes Included
Surfactant-Replacement Therapy

Field: Medicine (Health Sciences)

SURFACTANT-REPLACEMENT therapy for premature infants born with inadequate stores of pulmonary surfactants is here to stay. A growing number of prospective, randomized, controlled clinical trials testify to both the efficacy and safety of the tracheal instillation of surfactant in treating premature infants with the respiratory distress syndrome.1 Respiratory distress syndrome, also known as hyaline membrane disease, was previously the leading cause of death in live-born preterm infants. Approximately 10,000 deaths per year were recorded in the United States in the early 1970s; the number was closer to 5000 per year in the 1980s, before the advent of surfactant-replacement therapy. Respiratory . . .
Control of potato moth, Phthorimaea operculella (Zeller) in stored seed potatoes.

Field: Agricultural and Biological Sciences (Life Sciences)

Dust formulations of 'Bacillus thuringiensis' ('B. t.') (5,000 IU/mg), permethrin (0.1%), prothiofos (1%), chlorpyrifos (1%) and rotenone (2.4%) gave good protection of potato tubers from infestation by the potato moth, 'Phthorimaea operculella', in laboratory trials. In tubers infested with larvae, 'B. t.' permethrin, prothiofos and rotenone were significantly more effective than chlorpyrifos. Permethrin and 'B. t.' dusts had no effect on sprout production. In cold storage trials at 4 degreesC, viability of eggs steadily declined after 14 days to 42 days when only one per cent of eggs hatched. Cold storage of infested potato tubes caused complete mortality of four and seven day old larvae by 21 days and 35 days respectively. The use of dusts and cold storage in protecting seed tubers is discussed.
Groundnut under organic farming: Genetic variability and association studies for yield, quality and disease resistance in recombinant inbred lines

Field: Agricultural and Biological Sciences (Life Sciences)

A population consisting of 147 RILs derived from the cross TG 49 x GPBD 4 and their reverse cross consisting 20 RILs respectively (F<sub>7</sub> generation) were utilized for the study. Both the populations were subjected to phenotyping for quality traits (oil content and protein content), disease resistance (rust and LLS both at 70 and 80 days after sowing) and three productivity traits (pod yield/plant, 100-seed weight and shelling %) for <italic>kharif</italic> 2009. In both the segregating populations, the analysis of variance indicated significant variation for all the yield traits and diseases, but the variation was found to be less for quality traits. High genotypic and phenotypic variation was observed for pod yield (kg/plant), shelling per cent, rust and LLS at stage I it indicates the presence of considerable amount of genetic variability for these traits whereas in oil content and protein content there is low GCV and PCV. There was a highly significant and positive correlation between the protein and oil content in GPBD 4 x TG 49 population but negative relation in the TG 49 x GPBD 4 population. Highly significant positive correlation was observed in 100-seed weight and pod yield per plant in both the population indicating that breeding for high yield can be achieved without comprising the large seed size, which is a preferred trait for confectionery groundnut. Several RILs superior to best parent were identified for different traits which could be utilized in future breeding programmes.
Biogenesis of cytochrome b6 in photosynthetic membranes

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

In chloroplasts, binding of a c′-heme to cytochrome b6 on the stromal side of the thylakoid membranes requires a specific mechanism distinct from the one at work for c-heme binding to cytochromes f and c6 on the lumenal side of membranes. Here, we show that the major protein components of this pathway, the CCBs, are bona fide transmembrane proteins. We demonstrate their association in a series of hetero-oligomeric complexes, some of which interact transiently with cytochrome b6 in the process of heme delivery to the apoprotein. In addition, we provide preliminary evidence for functional assembly of cytochrome b6f complexes even in the absence of c′-heme binding to cytochrome b6. Finally, we present a sequential model for apo- to holo-cytochrome b6 maturation integrated within the assembly pathway of b6f complexes in the thylakoid membranes.
Daoism and Chinese Culture

Field: Arts and Humanities (Social Sciences)

A long-awaited textbook that introduces the major schools, teachings, and practices of Daoism, this work presents a chronological survey that is thematically divided into four parts: Ancient Thought, Religious Communities, Spiritual Practices, and Modernity. The work offers an integrated vision of the Daoist tradition in its historical and cultural context, establishing connections with relevant information on Confucianism, Chinese Buddhism, popular religion, and political developments. It also places Daoism into a larger theoretical and comparative framework, relating it to mysticism, millenarianism, forms of religious organization, ritual, meditation, and modernity. The book makes ample use of original materials and provides references to further readings and original sources in translation. It is a powerful resource for teaching and studying alike.
Deciding the Feasibility and Minimizing the Height of Tangles

Field: Mathematics (Physical Sciences)

We study the following combinatorial problem. Given a set of $n$ y-monotone Jordan curves, called wires, a tangle determines the order of the wires on a number of horizontal layers such that the orders of the wires on any two consecutive layers differ only in swaps of neighboring wires. Given a multiset $L$ of swaps (that is, unordered pairs of wires) and an initial order of the wires, a tangle realizes $L$ if each pair of wires changes its order exactly as many times as specified by $L$. List-Feasibility is the problem of finding a tangle that realizes a given list $L$ for a prescribed initial order if such a tangle exists. The Tangle-Height Minimization problem additionally aims to find a tangle that uses the minimum number of layers. List-Feasibility (and therefore Tangle-Height Minimization) is NP-hard [Yamanaka et al., CCCG 2018]. We prove that List-Feasibility remains NP-hard if every pair of wires swaps only a constant number of times. On the positive side, we present an algorithm for Tangle-Height Minimization that computes an optimal tangle for $n$ wires and a given list $L$ of swaps in $O((|L|/n^2+1)^{n^2/2} \cdot \varphi^n \cdot n! \cdot n \cdot \min\{|L|,n^2\}\cdot \log|L|)$ time, where $\varphi \approx 1.618$ is the golden ratio and $|L|$ is the total number of swaps in $L$. Obviously, this algorithm solves List-Feasibility, too. We show that List-Feasibility can also be solved by a simpler and faster version of the algorithm. Moreover, the algorithm helps us to show that List-Feasibility is in NP and fixed-parameter tractable with respect to the number of wires. For simple lists, where every swap occurs at most once, we show how to solve Tangle-Height Minimization in $O(n!\varphi^n)$ time.
Uptake of ~(131)I-FIAU in BMSCs infected by adenovirus vector-mediated HSV1-TK

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Report gene HSV1-TK and therapy gene were connected by IRES, and recombinant adenovirus vector Ad5-TK-IRES-BDNF-EGFP was constructed and infected with BMSCs at MOI of 0, 50, 100, 150, 200 and 250, with the control recombinant adenovirus vector of Ad5-EGFP. Green fluorescence cell positive rate was observed under the microscopy. MTT assay was used to determine the cell proliferation. bFGF and EGF were used to induce the BMSCs, and RQ-PCR to determine target gene expression in infection BMSCs. Uptake of 131I-FIAU was assessed by gamma counter. The data were processed by SPSS11.code. Recombinant adenovirus at MOI 150 had high infection efficiency and low toxic in BMSCs. There was a strong relation between the mRNA expression of TK and BDNF in infection BMSCs. The significance between the infection BMSCs and control BMSCs for uptake of 131I-FIAU at all the time points was t=23.06–173.83 and P0.05 (n=3). Recombinant adenovirus vector had high infection and low toxic for BMSCs, and effective uptake of 131I-FIAU. This suggests a suitable gene vector for tracing genetically modified stem cells.
Severe Aortic and Mitral Stenosis Secondary to Slowly Progressive Hunter Syndrome in an Elderly Patient

Field: Medicine (Health Sciences)

the typical findings of short stature, enlarged broad nose, thickened lips, and macroglossia.He had deficient iduronate 2-sulfatase enzyme activity in white cells.He had been weekly i.v.enzymatic replacement therapy A 62-year-old man with chronic atrial fibrillation was admitted to hospital with dyspnea on effort.He had a history of mucopolysaccharidosis type II (MPS II), also termed Hunter syndrome, and had
Gastroduodenal Tolerance of 75 mg Clopidogrel versus 325 mg Aspirin in Healthy Volunteers: A Gastroscopic Study

Field: Medicine (Health Sciences)

In contrast to aspirin, short-term treatment with clopidogrel does not induce macroscopic changes in the gastroduodenal mucosa. The study results show that in patients without gastroduodenal disease clopidogrel, but not aspirin, does not induce any gastroscopically evident erosions during short-term treatment.
Facts and figures: experience of a medical plan.

Field: Health Professions (Health Sciences)

The experience of the Midland Medical Plan for the years 1961--1971 was published in 1972, and this article is a continuation of those articles up to and including the anticipated figures for 1980. The figures reveal a different picture from that usually presented to the public by the media and by others, and suggest that health costs in the country are very reasonable in relation to the national economy.
Determination of Magnesium in Granulocytes

Field: Medicine (Health Sciences)

Determination of magnesium in mononuclear blood cells may provide useful information about intracellular magnesium. Mononuclear blood cell preparations are frequently contam-inated with granulocytes. We determined the normal refer-ence interval for magnesium content and concentration of granulocytes and compared it with magnesium in mononu-clear blood cells. The mean granulocyte magnesium concen-tration and content were 6.95 (SD 1.25) mmol/L and 3.11 (SD 0.54)fmol per cell, respectively, vs 10.95 (SD 1.70) mmol/L and 3.27 (SD 0.46) fmol per cell in mononuclear cells. The concentration of magnesium in granulocytes was signifi-cantly less (P &lt;0.001) than in mononuclear blood cells, but the magnesium contents were not significantly different. Magnesium has been measured in mononuclear blood cells to assess magnesium status associated with several disease entities (1-B), and this measurement may better indicate intracellular magnesium, total body magnesium, or both, than does determination of magnesium in serum or erythrocytes. Mononuclear blood cells isolated from whole blood have a concentration of magnesium second only to that in bone (9). Mononuclear blood cell preparations are occasionally contaminated with granulocytes, typically five per 100 cells or fewer. Greater contamination with granu-locytes (as high as 15 per 100 cells) may be found in certain clinical states such as pregnancy and labor (8). This vari-ability of the cellular composition of the lysatemay affect theresult. In this study, we simultaneously determined the magnesium content and concentration of granulocytes and mononuclear blood cells as a potential index for assessing magnesium status and evaluated the effect of granulocyte contamination on the result for mononuclear blood cell magnesium.
Mattering Moderates the Link between Gender Ideology and Perceived Fairness of the Division of Household Labor

Field: Psychology (Social Sciences)

This study examines the moderational role played by the feeling of mattering to the partner in the relationship between gender ideology and perceived fairness using a sample of 141 Canadian mothers with at least one child between the ages of two to five years old. Results support the moderator model and suggest that the emotional rewards mothers receive from their romantic partner influence the way they use gender ideology to evaluate the fairness of the division of family labor in their household. The results show that egalitarian gender ideology is associated with a greater sense of unfairness only when women feel that their partner demonstrates a low level of appreciation toward them. The findings are discussed in terms of the distributive justice theory.
Extracting alumina from bauxite ore in Jajarm process simulation using aspen software

Field: Chemical Engineering (Physical Sciences)

Installed DCS system in Jajarm alumina production factory in Iran, help to us for transferred the signals from equipments to simulators. The simulators can help to operator for control, monitoring and training. Authors have tried to find a new simulator using ASPEN plusTM software and write a new protocol for discussed tasks.Aspen PlusTM by Aspen Technology is one of the major process simulators that arewidely used in alumina process industries today. It specialises on steady-state analysis.For implementation, simulation and interpretive of the alumina process flowsheet in jajarm factory was used from the Aspen Plus simulator. We have tried to be able to provide a list key operating and design parameters and equipment selection criteria for a limited set of unit operations and processes. And so for determinanation the economic potential of a process and to identify the operational variables that affect the economics we have studied the process and flowsheets.Using the general information of factory, process informations same as temperature, measuredpressure and so PFD, cinitic informations and finallay clin, bauxite and sodium sulphateinforamations we have design a new simulation method for digestion and control process in Jajarmfactory. The process has implemented on LCR1,PU08, PU10 and PU11 units.
Educators as decolonial intellectuals: revolutionary thought from Gramsci to Fanon

Field: Arts and Humanities (Social Sciences)

For several decades, the Gramscian notion of the intellectual has been a popular framework to view the potentiality of educators as counter-hegemonic cultural workers. While this was an invaluable contribution to the field of critical education, notions of the intellectual have largely focused on class conflict. For a deeper theorization of the intellectual and race, we turn to the work of decolonial thinker Frantz Fanon. In his work, Fanon theorizes the role of the intellectual amid the struggle against colonialism. In this article, we examine Fanon's intellectual work as well as his writing on the 'colonized intellectual' to articulate what we describe as a Fanonian decolonial intellectual. We conclude by highlighting the importance of Fanon's contributions on the intellectual for educators of color, who presently find themselves compromised by a hegemony characterized by neoliberal multiculturalism in education.