1077 PROTEIN BIOMARKER ANALYSIS OF PRIMARY PEYRONIE'S DISEASE CELLS

Field: Medicine (Health Sciences)

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Peyronie's Disease/Surgical Therapy I1 Apr 20101077 PROTEIN BIOMARKER ANALYSIS OF PRIMARY PEYRONIE'S DISEASE CELLS Ling De Young, Anthony Bella, David O'Gorman, Bing S. Gan, Eric Chung, and Gerald Brock Ling De YoungLing De Young London, Canada More articles by this author , Anthony BellaAnthony Bella Ottawa, Canada More articles by this author , David O'GormanDavid O'Gorman London, Canada More articles by this author , Bing S. GanBing S. Gan London, Canada More articles by this author , Eric ChungEric Chung London, Canada More articles by this author , and Gerald BrockGerald Brock London, Canada More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2236AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The molecular pathogenesis Peyronie's Disease (PD) remains unclear more than 250 years after its initial description. Because of this, no test is currently available to accurately predict PD progression among those affected. The aim of this study was to investigate the expression of wound healing and fibrosis-associated proteins in primary cell cultures of PD fibroblasts to determine whether altered protein expression patterns can be used as predictors of clinical course and natural history. METHODS Primary cell cultures derived from normal Tunica albuginea tissue and PD plaque tissue were examined by Immuno-cytochemistry. Protein expression profiles were analyzed by Surface-Enhanced Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (SELDI TOF-MS) and Western immunoblotting. RESULTS Statistically significant increases in smooth muscle a-actin, b-catenin, and Hsp47 proteins were identified in cells derived from PD relative to cells derived from normal Tunica albuginea tissue. Changes in TGFb-1 receptor and Fibronectin were also observed. In addition, altered expression of additional as yet unidentified proteins at 4.7, 8.9, 10.8, 16.8 kDa and 76.8 kDa were detected by complementary SELDI TOF-MS approaches. CONCLUSIONS Primary cells derived from PD plaques display upregulated expression of several proteins that are established components of fibrosis and wound healing. In addition, changes in other, as yet unidentified proteins were measured. It will be of interest to conduct further studies to see whether these dys-regulated protein peaks represent potential biological markers of disease progression. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e419-e420 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ling De Young London, Canada More articles by this author Anthony Bella Ottawa, Canada More articles by this author David O'Gorman London, Canada More articles by this author Bing S. Gan London, Canada More articles by this author Eric Chung London, Canada More articles by this author Gerald Brock London, Canada More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...