jimnoneill/paper-to-field-training huggingface dataset:
Construction of eukaryotic expression vector of Interleukin-2 gene from Xiaoshan Chicken and its expression in Vero cells

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

In previous study IL-2 gene of Xiaoshan Chicken has been cloned by our laboratory, and in this study the cDNA of IL-2 was amplified and inserted into the eukaryotic expression vector pCI. The resulted recombinant plasmid pCI-IL-2 was transformed by electroporation into an attenuated Salmonella typhimurium strain ZJ111, which was then used to transfect Vero cells. DNA extracted from the transfected Vero cells showed a positive signal as hybridized with DIG-labeled probe in a DNA blotting assay, which revealed that the IL-2 gene was integrated into the genome of Vero cells. Specific yellow-green fluorescence was visual in indirect immunofluorescence assay with FITC-labeled goat anti-rabbit IgG. The 14.5 kD and 18.5 kD bands correspond to IL-2 protein from lysate of Vero cells was identified by SDS-PAGE and Western blotting, indicating that in Vero cells IL-2 was expressed successfully with the characteristics of immunological reactivity.
Exploring Palliative Nurses’ Perception of the ELNEC Program in Singapore: A Qualitative Study

Field: Nursing (Health Sciences)

Introduction: The ELNEC international curriculum was developed in 2000 and translated into many different languages for nurses worldwide. Although death is a universal phenomenon, handling death and dying differs across cultures and countries. Limited studies are conducted to explore the impact of ELNEC on nursing practices in Asia. Aim: This study explored palliative nurses' perception of the ELNEC and its impact on their nursing practices in Singapore long-term care setting Methods: An exploratory qualitative design using individual face-to-face interviews was conducted at a community hospital in Singapore between 2018 and 2019. An interview guide comprising four open-ended questions was used to generate participants’ feedback. Results: Three themes were generated from the content analysis: (i) lack of basic and continuing nursing education on death-related subjects; (ii) impact of ELNEC on nurses; and (iii) Perceived educational needs in palliative care. Conclusion: The ELNEC improved death anxiety and attitudes among existing nurses working in a community palliative setting. Specific educational needs were also identified as potential areas for enhancing the ELNEC for the Singapore palliative context. This study has demonstrated the need for ELNEC role within the national nursing curriculum for both nursing students and nurses in Singapore.
Development of a Telemedicine Acceptance Model in Indonesia by Considering Trust and Usability Factors for Self-Isolated Patients

Field: Health Professions (Health Sciences)

Telemedicine use during COVID-19 pandemic had significant impact in helping to deal with health problems, especially for self-isolation users. This study aims to develop a theoretical framework for model acceptance technology of telemedicine in Indonesia by considering aspects of trust and usability for self-isolation patients. Technology acceptance model (TAM) is used as a theoretical framework for this study involving several antecedents. Data was collected using Likert scale questionnaire and distributed online, receiving responses from 467 respondents. Partial Least Square-Structural Equation Model (PLS-SEM) analysis method used to explore factors related to behavioral intention and actual use. This study indicates a positive relationship between learnability and memorability on perceived ease of use. Then, effectiveness and trust have a positive relationship to perceived usefulness, but it is proven not to have a positive relationship to behavioral intention to use. Furthermore, the results show that contamination avoidance, perceived ease of use, perceived usefulness, reliability, and information quality significantly affect behavioral intention to use. As for safety and professionalism, it is not proven to have a positive relationship with behavioral intention to use. These findings can provide input for government and telemedicine application developers in considering user factors to anticipate patients using telemedicine in self-isolation.
The turbulent particle propagator in a magnetic field

Field: Physics and Astronomy (Physical Sciences)

The authors examine qualitatively the requirement that field-particle interactions in turbulence be diffusive. The basic assumption necessary for turbulently dissipative (resonantly broadened) models is that the field-particle correlation time viewed along the particle orbit be much smaller than the field-field correlation time in the laboratory. They assume the orbit variables x(t) and v(t) are normally distribution, but the electric fields need not be. They then use simple arguments from probability theory to derive the particle propagator for a weakly turbulent plasma in a strong magnetic field. They use this propagator to find the effects of cross field diffusion on single wave pulses and plasma echoes.
Cause-Effect Relations of Instruments of the Monetary Policy and Indicators of Sustainable Development of the Country Economy (Using Example of the NBU Discount Rate)

Field: Economics, Econometrics and Finance (Social Sciences)

The goal of the article is to study impact of the monetary policy on ensuring sustainable development of the country economy and detection of co-integration between the variables of the sustainable development of the country economy and instruments of the monetary policy. Systematisation, analysis and generalisation of scientific works allowed formation of an aggregate of indicators of sustainable development of the country economy for conducting a cause-effect relations analysis of indicators of sustainable development of the country economy and instruments of the monetary policy. The study results were used for identification of basic indicators of sustainable development of the country economy, which are sensitive to the impact of the instruments of the monetary policy. Using the models of mistake correction, the article reveals problem aspects of interaction of the NBU discount rate with capital investments, stimulation of which is one of the basic conditions of sustainable development of the country economy. Prospects of further studies in this direction are identification of interaction and interdependence of other instruments of the monetary policy with indicators of sustainable development of the country economy, building up forecasts and scenarios of ensuring sustainable development of the country economy using instruments of the monetary policy.
A model for cultivar classification and identification with reference to oats (Avena). I. Establishment of the groupings by taximetric methods

Field: Agricultural and Biological Sciences (Life Sciences)

This paper describes the establishment and validation of 14 groupings of cultivars in the hexaploid cultivated oats. This study is based on 5000 samples from various parts of the world. In order to base the groupings on non-agronomic characters, 21 attributes, mainly micromorphological ones taken from floral parts such as lemmas, paleas, and lodicules, were examined and recorded. A divisive chain algorithm was devised for clustering 5000 individuals. The 107 clusters thus formed were then processed by single linkage, yielding 14 acceptable groupings; these were subsequently investigated by a canonical analysis. The procedural principles proposed are described and discussed.
Engineering of complex systems: Understanding the art side

Field: Engineering (Physical Sciences)

The engineering of complex systems is both science and art. Although the cause of many difficulties in development of complex systems is the "art" side, this side is frequently ignored since it cannot be conveniently described and quantified. We recommend a conscious recognition of the appropriate role of the art, or judgment side of systems engineering, especially in the key activities of assumption and decomposition. Assumptions are the critical ingredients in both of the key processes of abstraction (representing only the most essential information) and of decomposition (dividing the problem into cleanly partitioned subsets). These two methods (abstraction and decomposition) form the core of any effective approach to engineering complex systems. © 1999 John Wiley & Sons, Inc. Syst Eng 3: 181–186, 1999
Some algorithms for determining an unknown initial position of AUV using information from a single beacon navigation system

Field: Computer Science (Physical Sciences)

Determination of the unknown initial position of an autonomous underwater vehicle (AUV) using information on the distance to a single mobile hydro-acoustic beacon (MHB) transported by an autonomous surface vehicle (ASV) is discussed. Three algorithms used to solve the problem are described. Some results of the algorithms performance obtained during the sea trials with the use of a marine autonomous robotic complex (MARC), including an AUV and ASV, are considered.
Utilisation of Pesticides by Soil Microorganisms

Field: Agricultural and Biological Sciences (Life Sciences)

While pesticides are considered somewhat essential in modern agriculture, their indiscriminate use has been linked to loss of biodiversity and ecosystem function as well as accumulation in food produce and the poisoning of groundwater. The utilisation of selected pesticides by microorganisms isolated from soil samples was observed. The pesticides tested were the organophosphorus insecticide – Diazinon and Herbicides – Primextra 500FW and Vetox 85. Detection of a zone of clearing was used to identify the Vetox 85-utilising microorganisms. Utilisers of Diazinon and Primextra 500FW were isolated by enriching the soil with mineral salt broth and providing Diazinon and Primextra as sole carbon and energy sources. This method was equally used for the in vitro degradation of the pesticides. Degradation was monitored using total viable cell numbers, pH and optical density. Generation times and growth rates of selected utilisers were determined. The Vetox 85-utilisers were found to be 0.32% of the total aerobic heterotrophic counts. The pesticide-degraders isolated were Vibrio, Acinetobacter, Pseudomonas, Arthrobacter, Flavobacterium, Bacillus, Aeromonas, Rhizopus and Penicillium species. Vibrio, Acinetobacter, Pseudomonas, Arthrobacter and Rhizopus were selected for degradation studies. Vibrio sp. showed the greatest pesticide utilisation capacity unexpectedly surpassing the mixed cultures, however, mixed cultures generally showed better degradative capacities than single cultures. Vibrio sp. had the highest growth rate while Rhizopus sp. had the lowest; Rhizopus sp. consequently showed the highest generation time alongside the mixed culture of Vibrio sp. and Acinetobacter sp. while Vibrio had the lowest generation time. The results showed that while these pesticides are relatively biodegradable in vitro, they are only utilisable by a limited number of indigenous soil microorganisms.
Development and testing of methodology for evaluating the performance of multi-input/multi-output digital control systems

Field: Engineering (Physical Sciences)

A Controller Performance Evaluation (CPE) methodology for multi-input/multi-output digital control systems was developed and tested on an aeroelastic wind-tunnel model. Modern signal processing methods were used to implement control laws and to acquire time domain data of the whole system (controller and plant) from which appropriate transfer matrices of the control system could be generated. Matrix computational procedures were used to calculate singular values of return-difference matrices at the plant input and output points to evaluate the performance of the control system. The CPE procedures effectively identified potentially destabilizing controllers and confirmed the satisfactory performance of stabilizing ones.
Pharmacy tin for powdered hartshorn

Field: Arts and Humanities (Social Sciences)

ID no.: KGZ 3780 Museum: The Museum of Pharmacy at the Jagiellonian University Medical College in Kraków https://muzea.malopolska.pl/en/objects-list/1718 Digitalisation: RDW MIC, Virtual Małopolska project Source: Objaverse 1.0 / Sketchfab
A hybrid approach for optimising the geometry and design of Ultra Wide Band (UWB) antenna

Field: Engineering (Physical Sciences)

This manuscript proposes a novel Ultra Wide Band (UWB) antenna in the dual-band rejection (DBR). The proposed technique is combination of hybridising Cat Swarm Optimisation (CSO) as well as Genetic Algorithm (GA); hence, it is called CSOGA technique. The UWB antenna is structured with 2.9–13.0 GHz frequency and to enhance the matching of impedance. The antenna type with size modification is determined by using optimisation algorithm. To attain the slits as well as strips band rejection in an antenna, the dual-band frequencies are rejected in 3.3–3.6 GHz as well as 5.15–5.825 GHz to band process of WiMAX, WLAN. Here, the efficiency of proposed technique is analysed by 10 standard benchmark functions (BF). The outcomes show that the proposed CSOGA technique gives 94% higher efficiency when likened to other techniques.
Political Patronage

Field: Social Sciences (Social Sciences)

Abstract Political patronage is the practice of rewarding supporters of a political leader, party, or organization with jobs, contracts, licenses, or other forms of payment or assistance. Until the late nineteenth century patronage was not only legal in the United States, it was a widely accepted means of mobilizing voters, strengthening political parties, and implementing policy agendas. Public sentiment began to turn against patronage in the late nineteenth century with the growing concern over post‐Civil War corruption in Washington and in some of the country's largest cities. Civil service reforms, changes in municipal government, and court rulings have reduced the blatant “spoils systems” for job seekers which flourished in the nineteenth century, but other forms of patronage continue to exist at the local, state, and federal levels. Contracts, licenses, and other benefits can be given to those who have provided political or financial support for parties and elected officials.
ChemInform Abstract: 5‐Fluoro‐1‐ (3,4‐dideoxy‐3‐fluoro‐6‐O‐trityl‐beta‐D‐glycerohex‐3‐eno‐pyranos‐2‐ulosy l) Uracil: Anticancer Agent

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Experience with transjugular liver biopsy.

Field: Medicine (Health Sciences)

The results of 193 transjugular liver biopsies performed with a modified needle are described. An adequate specimen was obtained in 97%, and complications were rare, although puncture of the liver capsule does occur and caused bleeding in two patients. Fever after the procedure was reduced by ultrasonic cleaning of the needle. Although not easy, this technique is safe and preferable in the management of selected patients, but in most patients percutaneous biopsy is to be preferred.
Geneti variation and selection among provenience of Quercus variabilis

Field: Agricultural and Biological Sciences (Life Sciences)

25 provenience of Quercus variabilis seeds were collected from its main distribution area in China.Raising seedlings experiments were carried out at Caichong Village,Huanggang Town,Feixi County,Anhui Province.Seedling growth traits and biomass were measured.The results showed that Q.variabilis seedling height and ground diameter growth showed an S curve,with July to September as the fast-growing period.Significant differences were observed on height,ground diameter,height to diameter ratio,biomass and roots from different Q.variabilis provenience.Height was significantly positive related to ground diameter,stems,leaves,total biomass.Significant positive correlation was also observed between ground diameter and root,stem,leaves,the total biomass.Similarly,roots were significantly positive correlated with the stem,leaves,biomass too.Principal component analysis showed that the main differences among provenience were decided by the ground diameter and biomass of various parts of the plant.Seven improved provenience,originated from Menghai of Yunan Province,Dongzhi of Anhui Province,Xingyang of Henan Province,Youyang of Chongqin city,Hangzhou of Zhejiang Province,No.2 Jinzhai of Anhui Province and Jixi of Anhui Province,were selected.
Protein Synthesis in the Desiccation Tolerant AngiospermXerophyta villosaduring Dehydration

Field: Agricultural and Biological Sciences (Life Sciences)

Leaves of the resurrection plant <it>Xerophyta villosa</it> appear to become desiccation tolerant while they dry on the intact plant. After a small decline during moderate water stress, the polyribosome content of attached leaves appears to rise at 50% relative water content (RWC) to almost double the content in controls, before it finally declines to zero at 20% RWC. Partition of leaf soluble protein by polyacrylamide gel electrophoresis indicates an increase in protein with low mobility in dehydrated leaves. Studies on the incorporation of 14C-valine and 3H-valine, and of proteins dissociated into their component polypeptddes suggest that protein synthesis during dehydration is at least partly responsible for the changes in soluble protein.
Diet overlap of non‐native alewife with native yellow perch and spottail shiner in nearshore waters of southwestern Lake Michigan, 2000–2007

Field: Agricultural and Biological Sciences (Life Sciences)

Abstract – Lake Michigan has experienced many ecological changes as a result of introductions of non‐native species. Arguably the most significant was that of alewife ( Alosa pseudoharengus ), yet studies on diet overlap with native species are lacking. We analysed diet trends of alewife, spottail shiner ( Notropis hudsonius ) and yellow perch ( Perca flavescens ) collected in summer and fall 2000–2007 near Waukegan, IL, in 3–10 m depths. Mean percentage composition by dry weight for 23 prey taxa was used in multivariate analysis to test whether diet differed across species and size classes. We also tested whether zooplankton and benthic invertebrate community composition changed over time. Fish diets were similar over all years but differed seasonally. In summer, diets of large alewife were similar to both small alewife and small yellow perch, with Bosminidae, chironomid larvae and copepods as primary common prey. During fall, alewife and yellow perch size classes exhibited strong intraspecific diet overlap, while there was low diet overlap between species. Primary distinctions between species’ diets in fall were higher consumption of amphipods by yellow perch and dreissenids by spottail shiners compared to alewife, which consumed higher proportions of zooplankton. Overall, high yellow perch diet overlap with alewife during summer and with their larger conspecifics during fall could lead to negative implications for yellow perch growth before the critical overwintering period. Detailed insights into diet overlap and prey availability are critical first steps in understanding competitive interactions between native and non‐native fish that dominate the nearshore community in southwestern Lake Michigan.
Synthesis and analysis of fuzzy logic finite state machine models

Field: Computer Science (Physical Sciences)

A common drawback of most linguistic models is that they are essentially static, time is not a parameter in describing the behavior of the process model, or they are unable to respond to a specific sequence of changes at the inputs of the system. Dynamic linguistic models can be implemented by fuzzy automata. Of the feasible solutions, at present, fuzzy logic RISC processors and fuzzy logic finite state machines (ASIC) seem to be most promising. The latter approach is taken into consideration. In this paper the model of the fuzzy-state-fuzzy-output (FSFO) finite state machine (FSM) is discussed in detail. The state membership function (SMF) is introduced and the FSFO FSM implementation by means of crisp-state-fuzzy-output (CSFO) FSM using SMF is proposed. The multivariable model and inference scheme are suggested based on CSFO and FSFO FSM.< >
Dynamics of thin liquid films on vertical cylindrical fibres

Field: Engineering (Physical Sciences)

Recent experiments on thin films flowing down a vertical fibre with varying nozzle diameters present a wealth of new dynamics that illustrate the need for more advanced theory. We present a detailed analysis using a full lubrication model that includes slip boundary conditions, nonlinear curvature terms and a film stabilization term. This study brings to focus the presence of a stable liquid layer playing an important role in the full dynamics. We propose a combination of these physical effects to explain the observed velocity and stability of travelling droplets in the experiments and their transition to isolated droplets. This is also supported by stability analysis of the travelling wave solution of the model.
Challenges in Imputation of ICU Time-Series Data: A Comparison of Classical and Machine Learning Approaches

Field: Computer Science (Physical Sciences)

Handling missing data is a major challenge in machine learning, particularly in clinical settings using electronic health records like ICU time-series data. Advanced imputation techniques, such as Bidirectional Recurrent Imputation for Time Series (BRITS), Self-Attention-based Imputation for Time Series (SAITS), and Multi-directional Recurrent Neural Network (M-RNN), show strong performance but are influenced by dataset characteristics like missing patterns and feature distributions. This study compares ten imputation methods on the public MIMIC-III database and COPRA, a local hospital dataset. We evaluate classical approaches (Zero, Mean, Median, Last), advanced models (BRITS, SAITS, M-RNN), and their transfer learning (TL) adaptations (TranBRITS, TranSAITS, TranM-RNN). TL was implemented by transforming COPRA to align with MIMIC-III, enabling fine-tuning of pre-trained models. TranSAITS achieved the best performance on the transformed dataset (M->C). Notably, the simple Last method also performed competitively for vital signs, underscoring its potential for real-time clinical use where low complexity and efficiency are key.