Anti-Diabetic Activity of Selected Medicinal Plant Extracts Used By Tribals in the Adilabad District of Telangana State By in Vitro

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Abstract Diabetes mellitus is a metabolic condition marked by high blood glucose levels as well as changes in carbohydrate, lipid, and protein metabolism. The goal of this study was to determine the inhibitory effect of selected plants (Artemisia vulgaris - root, and Angelica archangelica - leaves) on -glucosidase and -amylase to assess anti-diabetic effectiveness in vitro. By using the Soxhlet extraction method, each plant powder was repeatedly extracted with different organic solvents of increasing polarity. To investigate invitro anti-diabetic efficacy, the various solvent extracts were submitted to a -glucosidase and -amylase enzyme inhibition assay. Artemisia vulgaris stem bark crude extracts yielded 22.43 percent, 25.56 percent, 10.14 percent, 12.12 percent, and 1.10 percent in hexane, chloroform, ethyl acetate, acetone, and methanol, respectively. Angelica archangelica extracts were shown to have IC50 values of 57 g/ml, 43 g/ml, 63 g/ml, 64 g/ml, and 70 g/ml in n-hexane, chloroform, ethyl acetate, acetone, and methanol, respectively. In a dose-dependent way, all extracts reduced enzyme activity. Based on the IC50 values, Angelica archangelica chloroform extract was the most active of the two plant species, followed by Artemisia vulgaris. The chemicals responsible for A. archangelica promise in vivo anti-diabetic effect should be studied further. Key words - Angelica archangelica, Artemisia vulgaris, alpha-amylase, alpha-glucosidase. References Adisakwattana S, Lerdsuwankij O, Poputtachai U, Minipun A, Suparpprom C: Inhibitory activity of cinnamon bark species and their combination effect with acarbose against intestinal α -glucosidase and pancreatic α-amylase. Plant Foods Hum Nutr 2011, 66:143 – 148. Alberti KGMM, Zimmet PZ: Definition, diagnosis and classification of diabetes mellitus and its complications. 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