jimnoneill/paper-to-field-training huggingface dataset:
Development of a Hypodermic Needle-based Point-of-Care Device for the Detection of Vitamin-B12 and Vitamin-C from Cancer-Induced Sprague Dawley Rat Urine Samples

Field: Medicine (Health Sciences)

Vitamin deficiency is a common side effect of chemotherapy-induced cancer fatigue. The current antibody-based diagnostic kits are only used to measure the status of vitamins in cancer patients with painful procedures. And these tests are expensive and time-consuming. Hence, it is essential to measure the status of vitamins in cancer patients using point-of-care (POC) devices. We designed and developed an affordable, recyclable POC testing device in this study using ANSYS software version 2021 R2. The polyvinylidene fluoride (PVDF) membrane-based piezoresistive cantilever sensor was placed inside a hypodermic needle case. This sensor was coated with ascorbate oxidase (AO), hydrogen peroxide (H2O2), and luminol, and the oxidation of micronutrients in the tumor-induced rat's urine sample was measured using a digital multimeter. The maximum amount of deflection was observed when the sensor was loaded with vitamins B12 and C injected rat's urine. The assay exhibited good resistance responses toward target vitamins B12 and C. Moreover, our strategy provided acceptable reproducibility, precision, high specificity, and good accordance with the commercial kit for detecting micronutrients in cancer patients. This device helps detect the vitamins from a cancer patient's urine samples, a non-invasive way of carrying out sensitive diagnostic tests.
Apodization for systems of high f-number

Field: Physics and Astronomy (Physical Sciences)

The exit pupil distribution that maximizes the mean encircled energy fraction over some interval along a system's line of symmetry is found here for both two- and three-dimensional geometries. Such a merit function has been optimized previously by using the Debye model for the diffracted field, but this is not valid for the systems studied here; the more general Kirchhoff integral must be used. Optimal pupil functions for these systems are shown to depend on an additional parameter and represent a new family of solutions with nontrivial phase. Taylor series are derived for these functions, and they provide a powerful verification of the numerical results.
Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

The two-step process of selection and counter-selection is a standard way to enable genetic modi-fication and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression of tetA confers bacterial resistance to tetracycline (TcR) and also causes sensitivity to the lipophillic chelator fusaric acid; sacB causes sensi-tivity to sucrose. These two genes are introduced as a joint DNA cassette into Escherichia coli by selec-tion for TcR. A medium containing both fusaric acid and sucrose has been developed, in which, coexpression of tetA-sacB is orders of magnitude more sensitive as a counter-selection agent than either gene alone. In conjunction with the homolo-gous recombination methods of recombineering and P1 transduction, this powerful system has been used to select changes in the bacterial genome that cannot be directly detected by other counter-selection systems.
DENDRITIC CELL-BASED VACCINE THERAPY IN PATIENTS WITH RENAL CELL CARCINOMA

Field: Medicine (Health Sciences)

Objective: to study the efficiency and tolerance of autologous vaccine therapy based on dendritic cells (DC) in patients with renal cell carcinoma (RCC) and to examine changes in immunological parameters and their association with the efficiency of the therapy. Subjects and methods. Twenty-nine patients with RCC received autologous vaccine therapy based on DC in 2002 to 2008. Therapy was performed in the induction mode in 16 patients before disease progression and in the adjuvant mode (8 vaccinations) in 13 patients after radical nephrectomy (grade III) or radical metastasectomy. Peripheral blood monocyte-derived DCs treated with autologous tumor lysate were used to prepare the vaccine. Results. In a group of 16 patients with distant metastases, partial regressions were recorded in 2 (12.5%) patients and long (&gt; 6-month) stabilizations of a tumor process were observed in other 2 (12.5%) patients. The median time prior to progression was 3 (range 1.5-12) months. Thirteen patients on adjuvant treatment did not achieve the median time to progression: 4 patients showed no signs of disease progression ?12 to ?25 months after metastasectomy. Patients with a clinical effect (disease regression or long stabilization) showed a significant increase in the populations of CD3+CD8+ and CD3-CD16+ T lymphocytes (natural killers (NK) cells) after 3 vaccinations from 23.3 to 27.2% (p = 0.018) and from 15.17 to 20.3%, respectively (p = 0.03). Prior to vaccine therapy, the count of CD3+CD16+-NK cells was thrice greater in patients with the progressive disease than that in the donor group - 11.2 and 3.5%, respectively. The baseline count of CD4+CD25+ Т lymphocytes in patients with progressive disease was also significantly higher than that in patients with the clinical effect - 12.01 and 5.6%, respectively. Conclusion. In patients with RCC, DC-based vaccine therapy is able to induce a specific anti-tumor immune response that is transformed into the clinical effect in some cases. The baseline count of suppressor T lymphocyte (NKT and T-reg) populations may be a factor that predicts the efficiency of autologous DC-based vaccine therapy in patients with RCC. This immunotherapeutic approach merits further study, by defining the indications for its application in patients with RCC.
The use of nucleotide phosphorothioate diastereomers to define the structure of metal‐nucleotide bound to GTP‐AMP and ATP‐AMP phosphotransferases from beef‐heart mitochondria

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

The diastereomers of adenosine 5′‐O‐[1‐thio]triphosphate (ATP[αS]) and adenosine 5′‐O‐[2‐thio]triphosphate (ATP[βS]) were utilized to seek unambiguous assignment of Mg 2+ coordination to ATP when bound to ATP‐AMP phosphotransferase from beef heart mitochondria (AK 2 ). Similarly, the diastereomers of guanosine 5′‐O‐[thio]triphosphate (GTP[αS]) and guanosine 5′‐O‐[2‐thio]triphosphate (GTP[βS]) were utilized to seek unambiguous assignment of Mg 2+ coordination to GTP when bound to GTP‐AMP phosphotransferase from beef heart mitochondria (AK 3 ). Furthermore the diastereomers of guanosine 5′‐O‐[1‐thio]diphosphate (GDP‐[αS]) have been used to assign Mg 2+ coordination to GDP when bound to AK 3 . The ratios ( V for isomer S p )/( V for isomer R p ) obtained in the presence of Mg 2+ and Cd 2+ are compared to those already published for ATP‐AMP phosphotransferases from pig muscle (AK 1 ) [Kalbitzer et al. (1983) Eur. J. Biochem. 133 , 221–227] and from baker's yeast (AK y ) [Tomasselli and Noda (1983) Eur. J. Biochem. 132 , 109–115]. In all cases, coordination of Mg 2+ to the β‐phosphate via the pro‐R oxygen is present, as shown by reversal of specificity for the diastereomers of ATP[βS] or GTP[βS] respectively on changing the metal ion. In contrast, there is no reversal of specificity for the diastereomers of ATP[αS] or GTP[αS], or for GDP[αS] in the case of AK 3 for the reverse reaction, indicating that there is no interaction of the metal with the α‐phosphate group. The observed stereospecificity for the α‐thiophosphate is consistent with the assumption of an interaction of the pro‐R oxygen of the α‐phosphate group with the enzyme.
A Structural Taxonomy of Amateur Sport Organizations

Field: Social Sciences (Social Sciences)

The theoretical rationale underlying this study was that a variety of structural design types exist in amateur sport organizations and that their structural characteristics may be effectively measured, scaled, and compared. Characteristics were defined along three dimensions of organizational structure: specialization, standardization, and centralization. The approach used to identify the structural design types was the creation of an organizational taxonomy. Based on the measurement of 15 structural scales for 59 provincial sport organizations, Ward’s hierarchical fusion algorithm clustering technique was used to partition these data into homogeneous subsets. Analysis revealed 8 structural design types. The results, while providing support for the idea that there is a trend toward a more professional and bureaucratic form for amateur sport organizations, also suggest that it is important to consider the potential variety in the structural design of these organizations.
Model-Based Gaze Control

Field: Computer Science (Physical Sciences)

Gaze control is often defined as an operation on the attentive level, dealing with the selection and tracking of interesting parts of a scene. By using information derived from previously acquired object models, an active vision system can guide its gaze to perform more complex task. Possible applications are object inspection and face tracking for teleconferencing. The presented system acquires a frontal view of designated facial features with the help of a model image of a subject&apos;s face. 1 Introduction Current active vision systems operate mainly on the attentive level: they are concerned with selecting an interesting part of the scene to look at and maintaining acquisition of the found object. The issues that are typically addressed in such systems include low-level vision for object localization (e.g., [ Pahlavan et al., 1993; Reid et al., 1993 ] ) and visual servoing (e.g., [ Coombs, 1992; Corke, 1994 ] ) The control of gaze on the attentive level is a building bloc...
Silencing L-selectin expression by siRNA attenuated metastasis of murine lymphoid neoplasm cell P388D1 to peripheral lymph nodes

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

S Mishra, V Pertz, B Zhang, P Kaur, H Shimada, J Groffen, Z Kazimierczuk, LA Pinna and N Heisterkamp Department of Pediatrics, Division of Hematology/Oncology, Childrens Hospital Los Angeles Saban Research Institute and the Keck School of Medicine, University of Southern California, LA, USA; Department of Pathology, Childrens Hospital Los Angeles Saban Research Institute and the Keck School of Medicine, University of Southern California, LA, USA; Laboratory of Experimental Pharmacology, Department of Experimental Pharmacology, Polish Academy of Sciences Medical Research Center, Warsaw, Poland and Department of Biological Chemistry, University of Padova and Venetian Institute for Molecular Medicine, Padova, Italy E-mail: heisterk@hsc.usc.edu Current address: Department of Pediatrics, Division of Research Immunology and Bone Marrow Transplantation, Childrens Hospital Los Angeles, LA, USA.
Service Robot Acceptance Among Customers of Insurance in Kathmandu Valley

Field: Business, Management and Accounting (Social Sciences)

Purpose: Service robots equipped with automation and artificial intelligence, especially generative AI, are increasingly integrated into service interactions worldwide. Despite their advanced capabilities, research on factors influencing customer acceptance of service robots remains limited. To examine the acceptance of service robots among insurance customers in Kathmandu Valley, Nepal, using the sRAM framework. Design/Methodology/Approach: A crosssectional survey collected data from 243 insurance customers in Kathmandu Valley using selective sampling. Hypotheses were tested employing SPSS statistical analysis. Findings: Perceived Ease of Use (PEOU) and Subjective Social Norms (SSN) significantly impact customer acceptance of service robots. Similarly, Perceived Social Influence (PSI) and Robot Anxiety Perception (RAP) also have a significant positive influence. However, Perceived Usefulness (PU), Perceived Humanness (PH), Perceived Social Norms (PSN), and Trust (TR) showed no significant effect on acceptance among these customers. Practical Implications: This study enriches the literature on service robot adoption and offers actionable insights for Nepal’s insurance industry to optimize the deployment of robotic services. Originality: Among the first studies to investigate service robot acceptance specifically within Nepal's insurance customer context.
REACTIONS OF α-KETOSULFENES WITH CYCLIC NITRONES

Field: Chemistry (Physical Sciences)

Abstract Benzoylsulfene 1 and two cyclic α-ketosulfenes 2 and 3, generated in situ from the corresponding sulfonyl chlorides and triethylamine, react with 1-pyrroline 1-oxides to produce the corresponding cis-s-cis β-aminoenones and/or β-imino sulfonic acids, whose relative yields greatly depended on the nature of solvents employed. The reaction pathways are also described.
Assembling a Successful Industry-sponsored Senior Capstone Program: Lessons Learned from a Startup Effort at a Liberal Arts University

Field: Engineering (Physical Sciences)

A medium-sized (around 4000 students) liberal arts university recently began a general engineering program. The program’s first graduating class was in May 2016, and the program obtained initial ABET accreditation in August 2017. Entering the 2015-2016 academic year, program faculty envisioned a capstone design experience that would engage student teams in a year-long, professional level design project sponsored by an industry client. The first two years of the capstone design program have been inarguably successful, and in this paper we identify and reflect on the keys to our success. The intention for writing this paper is to ensure the success of the program is repeatable, and to assist other programs, especially those residing in small liberal arts universities, in starting or revising their own senior design experience. Our key factors in assembling a successful industry-sponsored capstone design program have been: (1) faculty buy-in and involvement, (2) engaged industry partners, (3) our teaming and project assignment strategy, (4) flexibility to address curricular concerns in program, and (5) building a corporate-like environment within the classroom. Early indicators of success include formal feedback from industry partners, feedback from our program’s industrial advisory board, and job placement of our program graduates.
Nanoparticle positioning effect on the percolation phenomenon of the polyethylene oxide/iron nanocomposite films

Field: Materials Science (Physical Sciences)

Abstract In this research, we demonstrate the influence of compelled iron nanoparticles (FeNPs) up to the surface on the percolation phenomenon and the electrical conductivity of the PEO/FeNPs nanocomposite films by applying a uniform magnetic field. The electrical conductivity of the PEO/FeNPs nanocomposite films exhibits a sharp increase from to 390 S∙cm −1 as the FeNPs volume fraction rises from 0.12 to 0.24, where the FeNPs form percolating networks and interparticle contacts. Applying a magnetic field drives the FeNPs towards the surface of the PEO/FeNPs nanocomposite films and consequently increases the electrical conductivity of the PEO/FeNPs nanocomposite films up to 1960 S∙cm −1 . The morphological features of the PEO/FeNPs nanocomposite films with different concentrations of FeNPs upon applying a magnetic field towards the surface were investigated. Finally, the corrosion protection efficiency of PEO/FeNPs nanocomposite films increases as FeNPs increase from an insulating zone to a conductive zone. On the other hand, applying a magnetic field drives the FeNPs towards the PEO/FeNPs nanocomposite surfaces, increasing the corrosion protection efficiency from 92.67% to 98.09%. Highlights The nanoparticle positioning effect on nanocomposite films is investigated. The electrical conductivity of PEO/FeNPs nanocomposite film reaches 390 S∙cm −1 . Electrical conductivity increases to 1960 S∙cm −1 upon nanoparticle positioning. Corrosion protection efficiency increases to 98.09% after nanoparticle positioning.
In vitro regeneration of Hypoxis colchicifolia plantlets

Field: Agricultural and Biological Sciences (Life Sciences)

Corms of Hypoxis species are heavily traded for use in traditional medicine in southern Africa. High demand has increased unsustainable harvesting from the wild, diminishing natural populations. Micropropagation of Hypoxis colchicifolia was investigated as a means of mass producing plants for both commercialization and re-establishment in the wild. Various plant organs were tested as explant sources and decontamination optimized for each explant type. Seeds failed to germinate in vitro, and inflorescence peduncles, leaves (young and mature) turned brown and did not respond in culture. Only 6% of corm explants produced callus and shoots. Flower buds responded best, with multiple shoots initiated from explants either directly from meristemoids or indirectly via callus. The problem of browning due to phenolic exudation was solved by including polyvinylpyrrolidone (PVP) in the culture medium when required. Common pathogens were partially controlled by washing affected explants in benomyl solutions. Rooting and corm induction were successful, and plantlets could be stored at low temperature (10 °C) prior to acclimatization with no adverse effects. In planting trials with 5-month-old and 21-month-old plants regenerated using the improved protocols, flowering percentage, corm and leaf size were increased significantly in plants grown in pots compared to those grown in the field over a 28 month period.
Mechanism Study of Photodamage to Photosystem during the Early Stages of Low Light Stress by Photonic Technique in Arabidopsis thaliana

Field: Agricultural and Biological Sciences (Life Sciences)

Low light limits photosynthesis by reducing the efficiency of photosynthesis in plants,thus causes the loss of agricultural production. Using photonic technique,the photodamage to photosystem under low light conditions were mainly studied,We found that plant growth phenotype and maximum light quantum yield( Fv / Fm) did not change significantly,the actual light quantum yield Y( II) and photosystem electron transfer efficiency( ETR) dropped more apparently during the early stages of low light stress. In addition,when protoplast were treated with low light,and then loaded with 2',7'-dichloro dihydrogen fluorescein diethyl( dichlorofluorescin diacetate,H2DCF-DA),a strong reactive oxygen species( ROS) accumulation could be detected,and which were mainly located in the chloroplast using laser scan confocal microscopy. The data may provide theoretic foundation for further studying the mechanism of low light tolerance in Arabidopsis thaliana.
The Role of the Transplant Coordinator

Field: Health Professions (Health Sciences)

The increasing success of pancreatic transplantation has resulted from a number of factors, many of which have been discussed in some detail in other chapters in this book. Perhaps less well appreciated has been the critical role played by the transplant nurse coordinator. The transplant coordinator establishes the initial contact with a patient interested in transplantation and follows the patient through the entire transplant process. The coordinator maintains the lines of communication among patient, referring physician, and all members of the transplant team, in order to ensure proper care of the patient before and after transplantation.
Immunohistochemical Evaluation of the Hedgehog Route as a Potential Prognostic Factor in Hepatocellular Carcinoma

Field: Medicine (Health Sciences)

ABSTRACT Introduction Hepatocellular carcinoma (HCC) is the most common primary malignant neoplasm in the liver. HCC develops gradually from multiple stages that control proliferation and apoptosis. In hepatocarcinogenesis, multiple signaling pathways were already described, such as the Hedgehog pathway (Hh). However, few studies have investigated the expression of Hh proteins as a potential prognostic factor in human HCC. This study aimed to investigate the expression of the Shh protein in HCC and to correlate with clinical and morphological prognostic characteristics of the tumor. Methods Immunohistochemical expression of Shh protein in tumor and cirrhotic parenchyma was performed in 36 HCC samples from patients who underwent liver transplantation at Clinical Hospital - UFMG. Correlation between the Shh tumor expression and etiology, number of nodules, size of the nodules, levels of alpha-fetus-protein (AFP), MELD score, tumor differentiation, and vascular invasion were performed. Results In our study, Shh protein labeling gradually increased from the normal to the cirrhotic and neoplastic parenchyma. Degree of tumor differentiation and vascular invasion were correlated with high Shh protein expression (p = 0.014 and p = 0.003, respectively). The other variables did not show a statistically significant correlation with Shh labeling. Conclusion Hedgehog pathway has importance in hepatocarcinogenesis. The immunohistochemical study of the Hh signaling pathway may have a promising role as a prognostic factor for HCC, especially due to the positive correlation between the Shh expression and the degree of tumor differentiation and invasion vascular.
Structural GenesandSelf-Catabolite Repression

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

phenotype causeeither an increase (hyperinducible), decrease (polar), or haveno effect atall on the inducible rateofexpression oftheL-arabinose operon.Fourteen araBgenemutants exhibiting sucheffects were showntobetheresult of:nonsense,frameshift, ormissensemutations. Allmissense mutantswere hyperinducible, exhibiting approximately a twofold increase inrateofL-arabinose isomerase production. Allframeshift andmostnonsensemutantsexhibited polar effect. Onenonsensemutantwas hyperinducible. Thecis-dominant polar effect ofnonsenseandframeshift mutants (ascompared toinduced wildtype) were more pronounced inarabinose-utilizing merodiploids andinaraBaraCc double mutantswhereinducible andconstitutive enzyme levels are respectively determined. On theotherhand,inarabinose-utilizing merodiploids, missense mutations no longer exhibited hyperinducibility but displayed a wild-type level ofoperonexpression. Increases inthewildtype-inducible rateofexpression oftheoperonwere foundwhengrowth ratewas dependent on theconcentration ofL-arabinose. Cyclic 3',5'-adenosine monophosphate also stimulated expression oftheoperonwiththewildtypeina mineral L-arabinose medium.Theseobservations are explained on thebasis thatthesteady-state expression oftheL-arabinose operonOIBAD isdependent on theconcentration of(i) L-arabinose, theeffector ofthis system, whichstimulates theexpression oftheoperon,and(ii) catabolite repressors, produced fromL-arabinose, whichdampenthe expression oftheoperon.We havetermedthelatter phenomenon self-catabolite repression.