jimnoneill/paper-to-field-training huggingface dataset:
BRCA1 and BRCA2 mutations in ovarian cancer patients from China: Association of ethnic-specific mutations in BRCA1 with an increased risk of ovarian cancer.

Field: Medicine (Health Sciences)

1583 Background: BRCA1/2 are cancer predisposition genes involved in hereditary breast and ovarian cancer (HBOC). Mutation carriers display an increased sensitivity to inhibitors of poly (ADP-ribose) polymerase (PARP). Despite a number of small-size hospital-based studies being previously reported, there is not yet, precise data of BRCA1/2mutations among Chinese epithelial ovarian cancer (EOC) pts. Methods: We performed a multicenter cohort study including 916 unselected consecutive EOC pts from eastern China, to screen for BRCA1/2mutations using the next-generation sequencing approach. Results: 153 EOC pts were found to carry pathogenic germline mutations in BRCA1/2, accounting for an overall mutation incidence of 16.7% with the predominance in BRCA1 (13.1%) compared with BRCA2 (3.9%). We identified 53 novel pathogenic mutations, among which the c.283_286delCTTG and the c.4573C>T of BRCA1 were both found in two unrelated patients. More importantly, the most common mutation, c.5470_5477del8 was most likely to be Chinese population-related without an apparent founder origin. This hot-spot mutation was presumably associated with an increased risk of EOC. Taken together, germline BRCA1/2mutations were common in Chinese EOC pts with distinct mutational spectrum compared to Western populations. Conclusions: Our study contributes to the current understanding of BRCA1/2 mutation prevalence worldwide. We recommend BRCA1/2genetic testing to all Chinese women diagnosed with EOC in order to identify HBOC families, to provide genetic counselling and clinical management for at-risk relatives. Mutation carriers may also benefit from PARP-targeted therapies. [Table: see text]
[The correction of metabolic acidosis by the instillation of 0.1N sodium hydroxide in an experimental model].

Field: Medicine (Health Sciences)

The critically ill patient, during the natural course of the disease often presents acid-base disturbances acidosis being the most common metabolic problem. Sodium bicarbonate has been used, for its correction but due to complications its use is restricted. In the present experimental study, mixed race dogs were artificially induced metabolic acidosis with in chloride acid. Arterial and venous blood gases were determined. Afterwards 0.1N sodium hydroxide was administered in the same amount statistically demonstrating modifications, in the pH values, as well as an excess of arterial and venous base which indicates the possibility of using this substance in humans to correct metabolic acidosis.
ANALISIS MOTIVASI PENGGUNAAN DOMPET DIGITAL OVO PADA MASYARAKAT DI DAERAH ISTIMEWA YOGYAKARTA

Field: Business, Management and Accounting (Social Sciences)

This study aims to determine the effect of perceived usefulness (PU), perceived ease of use (PEU), privacy and security (PS), and brand image to see the effects on behavioral intention (BI) and actual system use (ASU) on the use of OVO digital wallets in the community in the special area of Yogyakarta. This study uses a quantitative approach with the interval scale method. The number of samples in this study was 105 community respondents in the Special Region of Yogyakarta. The sampling method uses factor analysis techniques. The results of this study indicate that it has no significant effect, so the hypothesis is rejected. Variable testing using path analysis. Keywords: Perceived Usefulness; Perceived Ease of Use; Privacy and Security; Brand Image; OVO Penelitian ini bertujuan untuk mengetahui pengaruh perceived usefulness (PU), perceived ease of use (PEU), privacy and security (PS), dan brand image (citra merek) untuk melihat efek pada behavioral intention (BI) dan actual system use (ASU) terhadap penggunan dompet digital OVO pada masyarakat di daerah istimewa Yogyakarta. Penelitian saat ini menggunakan pendekatan kuantitatif dengan metode interval scale. Sampel diambil pada masyarakat di Daerah Istimewa Yogyakarta dengan total 105 orang. Hasil dari penelitian ini menghasilkan pengaruh yang signifikan, sehingga hipotesis diterima. Pengujian variabel dengan menggunakan analisis jalur. Kata Kunci: Perceived Usefulness; Perceived Ease of Use; Privacy and Security; Brand Image; OVO
Intravenous regional anaesthesia with ketamine

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Summary Ketamine, 40 ml 0.5% solution, was used in the technique of intravenous regional anaesthesia in 14 patients undergoing upper limb surgery. Satisfactory analgesia was obtained in 12 of the patients. The method is limited by the fact that all patients became unconscious within a few minutes of tourniquet release, and that this could not be prevented by naloxone.
Kinetic properties of mAMCase catalytic domain at various pH

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

This directory contains all files required to analyze the mouse AMCase enzyme kinetics across pH 2.0 to 8.0 presented in Figure 1 and Supplemental Figures 1 and 2 the manuscript Díaz et al., bioRxiv (2023). Enzyme kinetics were performed using a Tecan Spark multimode microplate reader and data was analyzed using Graphpad Prism. Figures were compiled using Adobe Illustrator. Files included in this directory: 20210906 - mAMCase_CatD_6xHis (pmRED006) - 20210907 - Purification contains FPLC chromatograms and corresponding SDS-PAGE gel stained with InstantBlue. - 202110XX _pHX_100nM contains four replicates performed at that specific pH using 100 nM mAMCase. - 384w_exp.toml contains a 384-well plate layout of the experimental conditions. This file is compatible with wellmap. - 384w_std.toml contains a 384-well plate layout of the 4MU standards. This file is compatible with wellmap. Figures
- contains PDFs of Vmax, kcat, KM, catalytic efficiency, and the initial rate for all pH points tested. 20210906 - mAMCase_CatD_6xHis (pmRED006) - Data.pzfx - contains all data from 20210906 - mAMCase_CatD_6xHis (pmRED006), including standard curves, initial time points, and initial rates curves. 20210906 - mAMCase_CatD_6xHis (pmRED006) - Summary.pzfx - contains summary data of kinetic parameters Vmax, kcat, KM, catalytic efficiency, and initial rates for all pH points tested. Contact:
Roberto Efraín Díaz, robertoefrain.diaz@ucsf.edu James Fraser, jfraser@fraserlab.com
CRISPRimmunity: an interactive web server for CRISPR-associated Important Molecular events and Modulators Used in geNome edIting Tool identifYing

Field: Computer Science (Physical Sciences)

Abstract The CRISPR-Cas system is a highly adaptive and RNA-guided immune system found in bacteria and archaea, which has applications as a genome editing tool and is a valuable system for studying the co-evolutionary dynamics of bacteriophage interactions. Here introduces CRISPRimmunity, a new web server designed for Acr prediction, identification of novel class 2 CRISPR-Cas loci, and dissection of key CRISPR-associated molecular events. CRISPRimmunity is built on a suite of CRISPR-oriented databases providing a comprehensive co-evolutionary perspective of the CRISPR-Cas and anti-CRISPR systems. The platform achieved a high prediction accuracy of 0.997 for Acr prediction when tested on a dataset of 99 experimentally validated Acrs and 676 non-Acrs, outperforming other existing prediction tools. Some of the newly identified class 2 CRISPR-Cas loci using CRISPRimmunity have been experimentally validated for cleavage activity in vitro. CRISPRimmunity offers the catalogues of pre-identified CRISPR systems to browse and query, the collected resources or databases to download, a well-designed graphical interface, a detailed tutorial, multi-faceted information, and exportable results in machine-readable formats, making it easy to use and facilitating future experimental design and further data mining. The platform is available at http://www.microbiome-bigdata.com/CRISPRimmunity. Moreover, the source code for batch analysis are published on Github (https://github.com/HIT-ImmunologyLab/CRISPRimmunity).
HbA1c variability in adults with type 1 diabetes on continuous subcutaneous insulin infusion (CSII) therapy compared to multiple daily injection (MDI) treatment

Field: Medicine (Health Sciences)

Objective To determine if continuous subcutaneous insulin infusion (CSII) therapy is associated with lower glycated haemoglobin (HbA1c) variability (long-term glycaemic variability; GV) relative to multiple daily injection (MDI) treatment in adults with type 1 diabetes mellitus (T1DM). Design Retrospective audit. Setting and participants Clinic records from 506 adults with T1DM from two tertiary Australian hospitals. Outcome measures Long-term GV was assessed by HbA1c SD and coefficient of variation (CV) in adults on established MDI or CSII therapy, and in a subset changing from MDI to CSII. Results Adults (n=506, (164 CSII), 50% women, mean±SD age 38.0±15.3 years, 17.0±13.7 years diabetes, mean HbA1c 7.8%±1.2% (62±13 mmol/mol) on CSII, 8.0%±1.5% (64±16 mmol/mol) on MDI) were followed for 4.1±3.6 years. CSII use was associated with lower GV (HbA1c SD: CSII vs MDI 0.5%±0.41% (6±6 mmol/mol) vs 0.7%±0.7% (9±8 mmol/mol)) and CV: CSII vs MDI 6.7%±4.6% (10±10 mmol/mol) vs 9.3%±7.3% (14±13 mmol/mol), both p<0.001. Fifty-six adults (73% female, age 36±13 years, 16±13 years diabetes, HbA1c 7.8%±0.8% (62±9 mmol/mol)) transitioned from MDI to CSII. Mean HbA1c fell by 0.4%. GV from 1 year post-CSII commencement decreased significantly, HbA1c SD pre-CSII versus post-CSII 0.7%±0.5% (8±5 mmol/mol) vs 0.4%±0.4% (5±4 mmol/mol); p<0.001, and HbA1c CV 9.2%±5.5% (13±8 mmol/mol) vs 6.1%±3.9% (9±5 mmol/mol); p<0.001. Conclusions In clinical practice with T1DM adults relative to MDI, CSII therapy is associated with lower HbA1c GV.
Abstract 3176: Modeling the complex multi-cellular ecosystem of the tumor microenvironment in colorectal cancer using multi-omics data

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Abstract The high inter-tumoral phenotypic heterogeneity of colorectal cancer exists within the tumor microenvironment (TME), which ultimately contributes to overall tumor survival. Tumor cells, as the center of the tumor microenvironment, employ sophisticated signaling networks to alter the cell fate decisions between cancerous and non-cancerous cells. A complex network of interactions is the source of this intercellular communication, resulting in tumor growth and progression, drug resistance, and ineffective treatment response. Recent experimental developments in colorectal cancer (CRC) have shown that understanding various underlying mechanisms of tumor growth and progression requires an in-depth understanding of the complex interactions between tumor cells and their surrounding microenvironment. Here, we analyze single-cell RNA sequencing data in CRC and construct gene regulatory networks to define interactions and identify cell states within the TME. The gene regulatory networks are incorporated as a multi-cellular ecosystem representing an experiment-based computational model of phenotypic crosstalk within the TME of CRC. The model simulates and predicts the multi-cellular responses to colorectal cancer to probe and identify mechanisms of cell state evolution and decisions resulting from chemo-agents, used to target TME components. This research will further our understanding of the multi-cellular crosstalk underlying the mechanisms within the TME and facilitate the development and optimization of cancer therapies that inhibit tumor growth in CRC. Citation Format: Geena Ildefonso, Stacey Finley. Modeling the complex multi-cellular ecosystem of the tumor microenvironment in colorectal cancer using multi-omics data [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 3176.
Aerodynamic characteristics of NACA 0012 airfoil section at angles of attack from 0 degrees to 180 degrees

Field: Engineering (Physical Sciences)

Report presenting the aerodynamic characteristics of the NACA 0012 airfoil section at a range of angles of attack from 0 to 180 degrees. Data were obtained at two different Reynolds numbers depending on whether the airfoil surfaces were smooth or had roughness applied at the leading and trailing edges. After the stall with the rounded edge of the airfoil foremost, a second lift-coefficient peak was obtained at an angle of attack of about 45 degrees.
Molecular Engineering of the Lead Iodide Perovskite Surface: Case Study on Molecules with Pyridyl Groups

Field: Materials Science (Physical Sciences)

We computationally investigate the molecular engineering approach of the lead iodide perovskite surface employing a pyridyl anchor-based molecular adsorbate as an example. The molecular adsorption approach on lead halide perovskite surfaces has been employed for passivation purposes in perovskite solar cells and was demonstrated to successfully enhance the solar cell performance in previous experimental studies. It is an open question whether the structures and properties of the lead halide perovskite can be further modified via the molecular engineering approach, and this study serves to probe the molecular engineering approach in the lead halide perovskite surface. First-principles calculations are employed to determine the nanoscopic structure of the lead halide perovskite surface with pyridyl anchor-based molecular adsorbates and prove that the pyridyl anchor-based molecule resides stably on the perovskite surface and modifies the perovskite surface structure. In addition, the calculations demonstrate that the electronic and optical properties of the lead halide perovskites can be controlled by the molecular engineering method. Noteworthily, we find that the molecular engineering approach is effective to modify the optical properties of the lead halide perovskite layer investigated in this study. Such molecular engineering approach on the perovskite surface could be potentially applicable to further enhance the performance of perovskite solar cells and perovskite-based optoelectronic devices.
Detection of pineapple closterovirus in pineapple plants and mealybugs using monoclonal antibodies

Field: Agricultural and Biological Sciences (Life Sciences)

Monoclonal antibodies (MAbs) were produced to the pineapple closterovirus (PCV) in Hawaii. These antibodies were shown to be specific for PCV by decoration of the virus particles in immunosorbent electron microscopy (ISEM) and indirect enzyme‐linked immunosorbent assay (ELISA). Several methods of ELISA were compared. An indirect DAS ELISA using a polyclonal antibody to trap virus particles followed by reaction with monoclonal antibody was shown to be the method of choice for detecting PCV in pineapple plants. Pineapple root tissue was found to be most suitable for detecting PCV in crude samples by indirect ELISA. PCV was detected in symptomatic and asymptomatic pineapple plants collected from Oahu and Maui, and pineapple collections in the USDA/ARS National Clonal Germplasm Repository, but was not detected from pineapple plants grown from seed. At least two serotypes of PCV were detected. In addition. PCV was detected from mealybugs collected from wilted pineapple plants, but not from mealybugs of the same species collected from a colony reared on squash. The role of PCV in mealybug wilt of pineapple is being investigated.
[Representative epidemiologic study of influence factors on the periodontal loss of attachment].

Field: Dentistry (Health Sciences)

The influence of age, inflammation intensity, tooth surface quality (caries, fillings), as well as mucogingival conditions on the loss of attachment was examined in 2000 probands. The loss of attachment was subdivided into periodontal pocket-formation and recessions. Determining cause is not the age but the inflammatory degree even if there is a significant increase of the attachment loss from age group to age group. The main part of the attachment loss increasing with advancing age is caused by recession development. Then, the individual dispersion is very high. Incorrect restorations, caries but also correct fillings increase periodontal disease. Mucogingival conditions show importance only in extreme case. There are less inflammations with pocket-formation than recessions. A preventive oriented basic care of the population is necessary to reduce irreversible damages of the periodontium.
The Good, the Bad, and the Ugly: A Critical Analysis of the U.S. Supreme Court Decision in Michael H. v. Gerald D

Field: Arts and Humanities (Social Sciences)

An attractive former model, D., separated from her husband in New York, meets a young man, Michael H., on the beach in Playa del Rey, California. A relationship develops, becomes pregnant by Michael, and a child, Victoria, is born. Michael's child by a pre vious marriage suffered from a serious genetic disorder, Lawrence Moon Beidel syndrome, which causes profound retardation.1 Michael and consult with the Genetic Clinic of the Univer sity of Southern California. Michael, and Victoria then go to a clinic at the University of California at Los Angeles for a Human Leukocyte Antigen (HLA) test to confirm Victoria's biological pa ternity. The HLA test demonstrated a 98.07% probability that Michael is Victoria's biological father.2 Michael assumes paternal responsibilities toward Victoria; he asks to divorce her husband and marry him. and Victoria subsequently move to St. Thomas, Virgin Islands, where and Michael lease a house together with signing the lease as Carole
Juncus sect. Tenageia in the Herbarium Centrale Italicum: confirmations and novelties for Italy. Video Abstracts, 115

Field: Agricultural and Biological Sciences (Life Sciences)

We carried out a revision of the Italian specimens belonging to Juncus L. subgenus Agathryon Raf. sect. Tenageia Dumort stored in the Herbarium Centrale Italicum of the Museum of Natural History of the University of Florence. This work allowed us to highlight several novelties concerning species distribution at the regional level.
Giant sacro-coccygeal teratoma. Case report.

Field: Medicine (Health Sciences)

A case is presented, of a giant teratoma in the sacro-coccygeal region, in a two-year old child. Surgery was performed in two successive stages, one month apart, under CT-scan control. Exeresis of the teratoma was complete. The authors stress the very large volume of the tumor, that extended in the pre-sacral area, and the fact that complete exeresis was mandatory. Eight months after surgery the postoperative evolution was satisfactory. The importance of CT-scans for the postoperative control is stressed, in cases of teratomas extending in the pre-sacral retroperitoneal area.
How does neighbourhood environment influence loneliness in later life in Urban China? The role of financial status

Field: Social Sciences (Social Sciences)

This study aimed to examine whether older adults' financial status moderated the association between neighbourhood environment (i.e. physical and social environment) and loneliness among older adults in urban China. A cross-sectional survey was collected from urban communities of Shanghai in mid-2020. The final analytic sample was 459 older adults (age range = 60-91 years; proportion of women: 55.8%). Multiple group analysis was applied to test the hypothesised model. A measurement model of community social capital (i.e. cognitive and structural dimensions of social capital), a crucial indicator of neighbourhood social environment, was first established in both high-income and low-income groups. Factor loading invariance was established in these two groups. The results show that structural social capital (i.e. organisation memberships, volunteering, social participation and citizenship activities) was negatively associated with loneliness among older respondents from the high-income group only, whereas better community security was significantly associated with lower levels of loneliness among low-income older respondents only. The findings imply that both structural social capital and household income should be considered as important indicators in needs assessment among lonely older adults. Future social policies and interventions should pay attention to enhancing the role of both social and physical neighbourhood environment among older adults.
The environmental and social governance of the Bui Dam project in Ghana

Field: Environmental Science (Physical Sciences)

This chapter examines the implications of the Chinese funding and construction of Bui Dam project and the emerging social and environmental governance issues. It discusses the findings obtained in relation to accessing natural resources and livelihood changes/impacts resulting from the social and environmental governance arrangements of the project. The chapter presents a description of the roles played by the different stakeholders, from international level to local level, in the construction of the Bui hydroelectric dam and in planning and implementation of resettlement, as well as in addressing challenges arising from the project execution. The Chinese construction firm Sinohydro built the dam as an engineering, procurement, and construction (EPC) contract. In the case of Bui, Sinohydro had to abide by Ghanaian labour laws, which meant inter alia allowing the formation of a union under the 2003 Labour Act. A major social issue emerging from the construction of Bui Dam is the re-alignment and breakdown in social structure of pre-dam construction society.
INITIAL CHARACTERIZATION OF HUMORAL IL-2 RESPONSE INHIBITION FOLLOWING DONOR-SPECIFIC TRANSFUSIONS UNDER AZATHIOPRINE IMMUNOSUPPRESSION1

Field: Immunology and Microbiology (Life Sciences)

A previous study indicated that noncompetitive humoral inhibition of IL-2-induced proliferation was a frequent consequence of donor-specific transfusions under azathioprine immunosuppression. In this report, we present our initial characterization of the mode of action and nature of this humoral activity. A direct role for azathioprine seems unlikely since its removal from post-DST+A sera did not eliminate inhibition, nor did addition of azathioprine to normal sera mimic the inhibition by post-DST+A sera. Inhibition of the proliferative response to IL-2 was observed on an individual cell basis after IL-2 stimulation that did not involve a direct effect on DNA synthesis. Inhibition appeared to require optimal IL-2 receptor expression as well as optimal doses of IL-2, suggesting that inhibition is manifested at the postreceptor level. Inhibition could not be removed by reconstitution with normal sera, indicating that post-DST+A sera were not deficient in a nominal serum component, which is necessary for optimal proliferation. The inhibitory activity in post-DST+A sera was sensitive to chemical reduction and heat. Inhibition of the response to IL-2 was readily demonstrated using IL-2 responsive human or murine T lymphocytes. However, post-DST+A serum also inhibited proliferation of one of two IL-2 independent cell lines. These results suggest that the inhibitory activity in post-DST+A plasma/serum may be due to the induction of an inhibitor of cell proliferation that is either lacking or "deficient" in normal serum.