jimnoneill/paper-to-field-training huggingface dataset:
Human trabecular meshwork organ culture: morphology and glycosaminoglycan synthesis.

Field: Medicine (Health Sciences)

Human corneoscleral explants were maintained for several weeks in defined, serum-free media. Trabecular cell vitality, as judged by vital stain exclusion, is high for at least one month. Trabecular ultrastructure, as compared to that of fresh eyes, first shows minor cellular and extracellular matrix degradation after 3 weeks in culture. The biosynthetic profiles of trabecular glycosaminoglycans (GAGs) change significantly by 3 weeks in culture. Eyes that are stored at 5 degrees C for up to 48 hr postmortem exhibit changes in trabecular ultrastructure and in GAG profiles; both characteristics return to normal by 7 days in culture. The incorporation pattern of 35S-sulfate and 3H-glucosamine into the GAGs of the trabecular meshwork (TM) is distinct from corneal or scleral incorporation. The relative incorporation of 3H-glucosamine into trabecular GAGs, as determined by sequential enzymatic degradation, is: 22.3% hyaluronic acid (HA), 27.9% chondroitin sulfate (CS), 21.3% dermatan sulfate (DS), 5.9% keratan sulfate (KS), 17.7% heparan sulfate (HS) and 4.9% unidentified material. The relative incorporation of 35S-sulfate into trabecular GAGs is: 0% HA, 32.9% CS, 34.8% DS, 7.7% KS, 13.8% HS and 11.1% into unidentified material. This profile is in good agreement with the profile that was previously obtained for human and nonhuman primate meshworks prior to culture. We conclude that corneoscleral explant organ culture is a useful tool for extracellular matrix studies within a time window from 7 to at least 14 days in culture.
Precursor engineering and cell physiological regulation for high level rapamycin production by Streptomyces hygroscopicus

Field: Chemical Engineering (Physical Sciences)

A process for high level production of rapamycin by Streptomyces hygroscopicus using statistical designs and feeding strategy was developed. The amino acids (i.e. Lys, Tyr, and Gln) for precursor supply were screened out in the initial phase of fermentation. The optimum levels determined with Box-Behnken design were Lys 20, Tyr 4, and Gln 3 g/l. In the rapamycin biosynthesis phase, the important component, ammonium sulphate, was also identified. A novel two-stage feeding strategy was developed successfully to increase the flux of rapamycin biosynthesis, in which the optimized amino acid components were fed in the initial phase of fermentation, and then switched to feed 2 g/l ammonium sulphate at 72 h. The maximal rapamycin production reached 860.6 mg/l in a 7 l fermentor, which was 182 % higher than that of the control. This was the first report to integrate precursor engineering and cell physiological regulation methods to optimize rapamycin production.
Western Delta University, Oghara 2022/2023,(Admission Form) Under graduate/Post Utme Form is Out ☎ 08067046209 -08067046209 Dr Mrs. Faith for more details on DE/Transfer Screening form,Remedial/Direct Entry Admission Form call (08067046209 Mrs Faith (0806)7046209 ,.This is to inform the general public that the PreDegree Application form 2022/2023 session is out on sale call (0)8067046209 mrs Faith (080)67046209 now

Field: Social Sciences (Social Sciences)

Western Delta University, Oghara 2022/2023,(Admission Form) Under graduate/Post Utme Form is Out ☎️ 08067046209 -08067046209 Dr Mrs. Faith for more details on DE/Transfer Screening form,Remedial/Direct Entry Admission Form call (08067046209 Mrs Faith (0806)7046209 ,.This is to inform the general public that the PreDegree Application form 2022/2023 session is out on sale call (0)8067046209 mrs Faith (080)67046209 now
Mitochondrial DNA mutations in oral squamous cell carcinoma S.L.Prior1, A.P.Griffiths2, J.M.Baxter1, P.W.Baxter3,

Field: Medicine (Health Sciences)

It has previously been demonstrated that mitochondrial DNA (mtDNA) mutations within the ND2 gene of histo-logically normal parotid salivary gland tissue of smokers may be molecular biomarkers for smoking-induced mtDNA damage. Oral squamous cell carcinoma (SCC) is strongly related to cigarette smoking; therefore, we used PCR and direct sequencing to establish whether mtDNA mutations were also present in oral SCC which could be used as additional biomarkers for smoking-associated DNA damage. In addition to searching for mutations in the ND2 gene, the mitochondrial D-Loop was also analysed. Three mutation hotspots were observed in the D-Loop at nt 146, 152 and 186, two of which (nt 146 and 152) have also been implicated in oesophageal SCC, another smoking-related cancer. The mutation hotspot observed at nt 186 has not previously been reported in other tumours. Furthermore, we show that the mutations previously reported within the ND2 gene in normal parotid tissue of smokers were not evident in these samples, but that a mutation hotspot occurs at nucleotide 4917 in oral SCC. We also show that D-Loop mutations occur predominantly in male smokers and female non-smokers and that this association with gender is statistically significant (P 0.003). We conclude that the mtDNAmutation hotspots found in this study, in particular nt 186, are potential biomarkers for oral SCC. However, owing to gender-specific differences in occurrence in smo-kers and non-smokers, and a lack of environmental smok-ing history, in general, it is difficult to associate these mutations with mtDNA damage induced by smoking. If the mutations observed in the subset of male patients are smoking induced, given our previous findings, mutation hotspots in the ND2 gene may be tissue specific suggesting the causative mutagens for mtDNA damage within these tissues are likely to be different.
Mechanisms Limiting Renal Tissue Protection and Repair in Glomerulonephritis

Field: Medicine (Health Sciences)

Glomerulonephritis are renal disorders resulting from different pathogenic mechanisms (i.e., autoimmunity, complement, inflammatory activation, etc.). Clarifying details of the pathogenic cascade is basic to limit the progression from starting inflammation to degenerative stages. The balance between tissue injury, activation of protective systems and renal tissue repair determines the final outcome. Induction of an oxidative stress is part of glomerular inflammation and activation of protective antioxidant systems has a crucial role in reducing tissue effects. The generation of highly reactive oxygen species can be evaluated in vivo by tracing the inner-layer content of phosphatidyl ethanolamine and phosphatidyl serine in cell membranes. Albumin is the major antioxidant in serum and the level of oxidized albumin is another indirect sign of oxidative stress. Studies performed in Gn, specifically in FSGS, showed a high degree of oxidation in most contexts. High levels of circulating anti-SOD2 antibodies, limiting the detoxyfing activity of SOD2, have been detected in autoimmune Gn(lupus nephritis and membranous nephropathy) in association with persistence of proteinuria and worsening of renal function. In renal transplant, high levels of circulating anti-Glutathione S-transferase antibodies have been correlated with chronic antibody rejection and progressive loss of renal function. Annexins, mainly ANXA1 and ANXA2, play a general anti-inflammatory effect by inhibiting neutrophil functions. Cytosolic ANXA1 is decreased in apoptotic neutrophils of patients with glomerular polyangitis in association with delayed apoptosis that is considered the mechanism for polyangitis. High circulating levels of anti-ANXA1 and anti-ANXA2 antibodies characterize lupus nephritis implying a reduced anti-inflammatory effect. High circulating levels of antibodies targeting Macrophages (anti-FMNL1) have been detected in Gn in association with proteinuria. They potentially modify the intra-glomerular presence of protective macrophages (M2a, M2c) thus acting on the composition of renal infiltrate and on tissue repair.
Neoadjuvant multimodal treatment of borderline resectable (BRPC) and locally advanced unresectable pancreatic cancer (LAPC) in Mexico.

Field: Medicine (Health Sciences)

454 Background: Pancreatic cancer remains a highly lethal disease. There is no consensus on treatment sequences and chemotherapy (CT) regimen in BRPC and LAPC. Our aim was to describe the multimodal treatment and outcomes in our population. Methods: We retrospectively reviewed medical records of patients (pts) with BRPC/LAPC and histological diagnosis of adenocarcinoma evaluated at Instituto Nacional de Ciencias Médicas y Nutrición from January 2011-December 2016. Clinical and pathological variables at diagnosis and treatment were recorded. Overall survival (OS) was estimated using Kaplan-Meier method and compared by Log-rank test. Results: 69 pts were evaluated, 39% (27) did not receive treatment. We analyze 42 treated pts. BRPC 33%/LAPC 67%. Median age was 58.8 y/o, 54.8% were female. Symptoms at diagnosis: 79% abdominal pain, 76% weight loss, 55% jaundice. ECOG performance status (PS): 0 (17%), 1 (69%) and 2 (14%). Main location was pancreatic head (76%). Median laboratory values: total bilirubin 1.04 mg/dL (0.2-25), albumin 4.1 g/dL (2.4-5.1), CA 19.9 182.8 U/mL (0.8-4028). Laparotomy at diagnosis was performed in 21%. All pts received induction CT (iCT). FOLFIRINOX was the most common regimen (37%), followed by FOLFOX4 (34%). The best overall response with iCT was stable disease (62%), progressive disease was observed in 24%. iCT followed by chemoradiation (CRT) could be delivered to 48% (20/42). Capecitabine-based CRT was preferred (94%). Six pts (14%) underwent surgical resection after multimodal treatment (36% BRPC, 3.5% LAPC), 5 pts achieved R0 resection. The resection rate with single-agent iCT was 0% vs 20% with combination iCT. Median OS was 15.6 months (m): 14.4 m for BRPC and 15.5 m for LAPC. Median OS according iCT: gemcitabine 7.8 m, fluorouracil 13.7 m, FOLFOX4 15.5 m and FOLFIRINOX 24.6 m. Univariate analysis identified ECOG PS (0-1 vs 2, P = 0. 014) and age ( < 59 vs ³59, P = 0.002) as significantly associated with survival. Conclusions: Early administration of combination CT followed by CRT and/o surgical resection in selected pts improves oncological outcomes in pts with BRPC/LAPC. In pts with good PS, iCT with FOLFIRINOX is the preferred regimen given best results.
4 Shuttle Orbiter Production Change Impact Assessment Process ‐ A Collaborative Team Effort

Field: Engineering (Physical Sciences)

Abstract The present Space Shuttle program is one in transition ‐ both from the government agency and the contractor level. NASA is restructuring the management of the Space Shuttle Program by turning over the reins of the day‐to‐day operations to a prime contractor, United Space Alliance (USA) ‐ a joint venture partnership with Boeing and Lockheed Martin as major subcontractors. This move is intended to save millions of dollars in costs by eliminating duplicate resources, streamlining operations, and reducing nonessential program requirements. Most separate shuttle related contracts have been consolidated into one Shuttle Program. While this consolidation effort is taking place, NASA, USA, Boeing are working closely together to provide the American public a Space Shuttle Program that continues to be technically viable, safe, and now more cost effective. Central to organizational consolidation is the way people think and do their work. Changes have been made emphasizing working together as teams and improving the processes they use. A critical process, that was changed, is the Orbiter modifications and upgrades change process. This process covers all changes to the Orbiter hardware, which fix flight to flight anomalies (modifications) as well as make upgrades, which increase safety, meet upcoming manifests, improve supportability and reduce costs. This paper deals specifically with process improvements made to the change control process methodology and implementation procedures (known as the change impact assessment process) by NASA, USA and Boeing to be more proactive and cost effective.
The Use of Isotopes in Acid and Basic Catalysis.

Field: Chemistry (Physical Sciences)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Use of Isotopes in Acid and Basic Catalysis.W. F. K. Wynne-JonesCite this: Chem. Rev. 1935, 17, 1, 115–123Publication Date (Print):August 1, 1935Publication History Published online1 May 2002Published inissue 1 August 1935https://pubs.acs.org/doi/10.1021/cr60056a009https://doi.org/10.1021/cr60056a009research-articleACS PublicationsRequest reuse permissionsArticle Views140Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Staged Repair of a Ruptured Thoracic Aortic Aneurysm Having an Aorto-Parenchymal Fistula

Field: Medicine (Health Sciences)

Ruptured thoracic aortic aneurysms, having aorto-bronchial or pulmonary parenchymal fistulations, are life-threatening conditions, and current surgical treatment results are not satisfactory. Herein, we describe a 69-year-old man with a ruptured thoracic aortic aneurysm and aorto-pulmonary parenchymal fistulation that was successfully treated using a staged repair. The first stage involved an emergency thoracic endovascular aortic repair for salvage and the second stage involved radical pulmonary parenchymal repair with removal of the stent graft and aortic reconstruction for long-term survival.
[Interaction of ATP with sarcoplasmic reticulum Ca2+-ATPase; effect on the conformational state of the enzyme].

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

It was studied how temperature influences the NBD-Cl inactivation of sarcoplasmic reticulum Ca2+-ATPase and the protective effect of ATP under conditions preventing ATP hydrolysis. Two types of ATP-binding sites with Kd equal to 30 and 220 microM at 37 degrees C were found. ADP interacts with these sites with the (K'd = 20 and 200 microM). The temperature decrease from 25 degrees to 5 degrees C induces the abrupt increase in the Kd for the low affinity site. The possible reasons for heterogeneity of ATP-binding sites are discussed. The conclusion is made that interaction of monomers in oligomeric complex of Ca2+-ATPase induces heterogeneity of ATP-binding sites.
Fault Location in Distribution Feeders with Distributed Generation using Positive Sequence Apparent Impedance

Field: Engineering (Physical Sciences)

Abstract: The existing methods of fault location for distribution systems consider that the system has a radial power flow, in other words, they don’t consider the presence of another generator in the distribution line. Thus, in systems with the presence of distributed generation, these methods show to be inefficient. In this paper, it is presented a new fault location method based on positive sequence apparent impedance. Computational simulations were made and the method was tested and compared with other existing fault location techniques in order to validate the method. The basic characteristics of the method, the new algorithm and a variety of case studies are presented in the paper in order to illustrate its efficiency.
Investigation of pulsed lasing in iron vapor

Field: Physics and Astronomy (Physical Sciences)

An experimental investigation was made of a self-heated laser utilizing a transition from a resonance to a metastable level in an iron atom at the 452.9 nm wavelength. The feasibility of pulsed lasing due to other transitions was analyzed. The average lasing power was 37 mW and the peak power was 1.2 kW.
Adrenergic Innervation of the Left Side of the Guinea Pig Heart

Field: Neuroscience (Life Sciences)

Histological, histochemical fluorescence, and electron microscopic techniques have been utilized to demonstrate the specific distribution of the noradrenergic sympathetic postganglionic fibers to the guinea pig left atrium and ventricle. The majority of these nerves entered the base of the heart. Paravascular bundles of unmyelinated sympathetic postganglionic fibers traversed the left atrioventricular sulcus. From these paravascular plexuses axons accompanied the arteries via periarterial plexuses to the deeper layers of the heart wall. The vascular tree was extremely complex. In the left atrium a relatively abundant noradrenergic innervation was seen within the interstitial connective tissue surrounding the cardiac muscle. In the left ventricle, most of the noradrenergic innervation was located in the tunica adventitia of all the major coronary arterial branches before they entered the cardiac muscle. These larger branches of the coronary arteries were predominantly innervated by the sympathetic postganglionic fibers. Apparently it is from these perivascular nerve plexuses that the left ventricular muscle receives the majority of its noradrenergic innervation.
Antibiotic Susceptibility Testing at a Screen-Printed Carbon Electrode Array

Field: Chemical Engineering (Physical Sciences)

Screen-printed carbon electrode arrays were treated to allow respiratory activity-based measurement of antibiotic susceptibility with Escherichia coli JM105. Carbon working electrodes were examined for antibiotic adsorption and were pretreated with various electrochemical and chemical protocols to minimize antibiotic adsorption. Treatment by voltammetry in basic solution or by chemical modification with poly-L-lysine or chitosan were found to be effective methods for the elimination of adsorption of the studied group of 17 antibiotics, which comprised several classes and modes of action. Measurements consisted of two-electrode amperometry of the bacterial suspension after 10 min of incubation with antibiotic followed by addition of an oxidative cocktail of ferricyanide and dichlorophenolindophenol for a further 10 min; response currents, which indicate the extent of reduction of ferricyanide to ferrocyanide by cellular respiratory activity, decrease with increasing concentration of antibiotic present in the initial 10 min incubation. IC50 values obtained for chloramphenicol with these electrode modification methods are consistent at 2.0 +/- 0.2 mM, in approximate agreement with previously reported respiration-based results for this organism but significantly higher than values reported for growth-based antibiotic susceptibility testing methods.
Effects of Aqueous Leaf Extract of Simarouba glauca DC (Simaroubaceae) on Lipoprotein Homeostasis and Oxidative Stress Biomarkers

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Background and Purpose: Simarouba glauca is widely reported to contain a number of biologically active compounds with potentials in the treatment of numerous diseases. The study was conducted to evaluate the sub-acute effects of the aqueous leaf extract of Simarouba glauca (AESG) on lipoproteins and oxidative stress biomarkers in male Wistar rats. Methods: Oral administration of AESG was carried out in line with the guidelines of the Organization for Economic Co-operation and Development (OECD), No. 425 using a total of 24 male Wistar rats allotted to four groups (n=6); given distilled water, 500, 1000, and 2000 mg/kg/day of AESG respectively for 30 days. Results: In plasma, there was a significant reduction (P?0.05) in HDL-cholesterol; elevated (P?0.05) triglycerides (TG) at 1000 and 2000 mg/kg/day; elevated (P?0.05), and LDL-cholesterol at 500 and 1000 mg/kg/day, relative to the control. While the level of liver total cholesterol (TC) reduced significantly, it increased in the heart. Catalase (CAT) activity in the liver increased significantly (P?0.05) at all doses. The dose of 1000 mg/kg/day significantly (P?0.05) elevated kidney CAT activity. The activities of superoxide dismutase (SOD) in liver and heart reduced (P?0.05) at 500 mg/kg/day. At all doses, the levels of reduced glutathione (GSH) in plasma, liver and heart were comparable with the control. Although, there were no significant changes in plasma and liver glutathione peroxidase (GSH-PX) activity at all doses, animals given 500 mg/kg had reduction (P?0.05) in the heart GSH-PX activity compared to the control. Conclusion: Oral sub-acute AESG at high doses altered lipid homeostasis in plasma and heart without lipid peroxidation or oxidative stress. The extract has the potential to cause hyperlipidemia.
Anonymity and the Textual Construction of Authority in Prosimetrum

Field: Arts and Humanities (Social Sciences)

The ways in which anonymity participates in the textual construction of authority is the focus of this chapter, which proceeds through an investigation of the literary effects that were achieved in two different kinds of medieval Icelandic prosimetrum, examples which demonstrate how literate authors exploited the potential of orally transmitted poetry to enrich their prose.The case studies are drawn from Gylfaginning by Snorri Sturluson, where anonymous poetry simulating the speech of gods is quoted within a treatise by a named author, and Þorgils saga ok Hafliða, where stanzas by contemporary poets named and unnamed are quoted within an anonymous saga about a 12 th -century political feud.The theoretical frame is provided by reflections on authorship by Michel Foucault and Roland Barthes which, while dating from the 1960s, still hold considerable relevance for the analysis of the competing voices of prosimetrum, especially with regard to the establishment of authority within written discourse.The notion of 'an index of truthfulness', constituted by poetic quotation around which the narration develops, is explored and it is proposed that across a wide range of discursive situations voices speaking according to conventions of poetic composition are rendered authoritative through the performance of quotation.
Clinical Evaluation of Iron Metabolism in Pancreatic Disease

Field: Medicine (Health Sciences)

In order to estimate the possible influence of pancreatic disease upon iron absorption, fecal analysis was carried out after administration of 5 mg iron labeled with 59Fe. Twelve cases of pancreatic calcification, 7 cases of chronic pancreatitis, 3 cases each of pancreatectomy and miscellaneous diseases, and 52 cases for control were studied. Significant elevation of iron absorption was observed in 4 or 21 patients with pancreatic diseases. Two of the 4 cases were also found to have low transferrin saturation and the other two have had a long history of heavy alcoholic intake. There was no definite correlation between the iron absorption rate and the total bicarbonate secretion, and duration of the diseases. Three cases treated with pancreatectomy failed to show increased iron absorption. The alternative administration of 59Fe and 55Fe for consecutive 4 days either alone or together with pancreatin or secretin was done to see the effect of these preparations on iron absorption. No significant inhibitory effect of these preparations on the iron absorption was noticed. These observations suggest that exocrine pancreatic function seldom appears to influence directly iron absorption, and that iron deficiency and ingestion of alcoholic beverages can be important factors affecting the iron absorption in pancreatic diseases.