Additional file 1 of Growth and longevity modulation through larval environment mediate immunosenescence and immune strategy of Tenebrio molitor

Field: Agricultural and Biological Sciences (Life Sciences)

Additional file 1: Table S1. Best models initially including Growth duration according to ΔAIC for Cellular immunity component (approached by the coordinates on the first principal component of an ACP) the individuals grown in the different temperature conditions (Larval environment: T20, T24 and T28). The worst model is given for information. The most completed model tested contained: the Growth duration, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Growth duration, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S2. Best models initially including Growth duration according to ΔAIC for Enzymatic immunity component (approached by the coordinates on the second principal component of an ACP) the individuals grown in the different temperature conditions (Larval environment: T20, T24 and T28). The worst model is given for information. The most completed model tested contained: the Growth duration, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Growth duration, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S3. Best models initially including Growth duration according to ΔAIC for Antibacterial activity component (approached by the coordinates on the third principal component of an ACP) the individuals grown in the different temperature conditions (Larval environment: T20, T24 and T28). The worst model is given for information. The most completed model tested contained: the Growth duration, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Growth duration, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S4. Best models initially including Adult longevity according to ΔAIC for Cellular immunity component (approached by the coordinates on the first principal component of an ACP) the individuals grown in the different temperature conditions (Larval environment: T20, T24 and T28). The worst model is given for information. The most completed model tested contained: the Adult longevity, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Adult longevity, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S5. Best models initially including Adult longevity according to ΔAIC for Enzymatic immunity component (approached by the coordinates on the second principal component of an ACP) the individuals grown in the different temperature conditions (Larval environment: T20, T24 and T28). The worst model is given for information. The most completed model tested contained: the Adult longevity, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Adult longevity, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S6. Best models initially including Adult longevity according to ΔAIC for Antibacterial activity component (approached by the coordinates on the third principal component of an ACP) the individuals grown in the different temperature conditions (Larval environment: T20, T24 and T28). The worst model is given for information. The most completed model tested contained: the Adult longevity, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Adult longevity, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S7. Best models initially including Growth duration according to ΔAIC for Cellular immunity component (approached by the coordinates on the first principal component of an ACP) the individuals grown in the different relative humidity conditions (Larval environment: H55, H70 and H85). The worst model is given for information. The most completed model tested contained: the Growth duration, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Growth duration, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S8. Best models initially including Growth duration according to ΔAIC for Enzymatic immunity component (approached by the coordinates on the second principal component of an ACP) the individuals grown in the different relative humidity conditions (Larval environment: H55, H70 and H85). The worst model is given for information. The most completed model tested contained: the Growth duration, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Growth duration, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S9. Best models initially including Growth duration according to ΔAIC for Antibacterial activity component (approached by the coordinates on the third principal component of an ACP) the individuals grown in the different relative humidity conditions (Larval environment: H55, H70 and H85). The worst model is given for information. The most completed model tested contained: the Growth duration, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Growth duration, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S10. Best models initially including Adult longevity according to ΔAIC for Cellular immunity component (approached by the coordinates on the first principal component of an ACP) the individuals grown in the different relative humidity conditions (Larval environment: H55, H70 and H85). The worst model is given for information. The most completed model tested contained: the Adult longevity, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Adult longevity, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S11. Best models initially including Adult longevity according to ΔAIC for Enzymatic immunity component (approached by the coordinates on the second principal component of an ACP) the individuals grown in the different relative humidity conditions (Larval environment: H55, H70 and H85). The worst model is given for information. The most completed model tested contained: the Adult longevity, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Adult longevity, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect. Table S12. Best models initially including Adult longevity according to ΔAIC for Antibacterial activity component (approached by the coordinates on the third principal component of an ACP) the individuals grown in the different relative humidity conditions (Larval environment: H55, H70 and H85). The worst model is given for information. The most completed model tested contained: the Adult longevity, the Age at measurement (young: ~15 days of adult stage or older: ~45 days of adult age), Challenge (before or after the immune challenge), the mass before the immune challenge, two and three variables interactions between Adult longevity, Age at measurement and Challenge. Models were linear mixed models with Larval environment and Individual as random effect.