jimnoneill/paper-to-field-training huggingface dataset:
Contribution of Virus-Like Particles to the Immunogenicity of Human Immunodeficiency Virus Type 1 Gag-Derived Vaccines in Mice

Field: Immunology and Microbiology (Life Sciences)

ABSTRACT The human immunodeficiency virus type 1 (HIV-1) Gag protein is a major target antigen for cytotoxic-T-lymphocyte-based vaccine strategies because of its high level of conservation. The murine model has been used extensively to evaluate potential HIV-1 vaccines. However, the biology of HIV-1 Gag is somewhat different in human and murine tissues. The ability of HIV-1 Gag to form virus-like particles (VLPs) in human cells is severely curtailed in murine cells. Hence, it is not known whether immunizing mice with expression vectors encoding HIV-1 Gag provides an accurate assessment of the immunogenicity of these candidate vaccines in primates. In this report, we made use of a chimeric Moloney murine leukemia virus (MMLV)-HIV-1 Gag in which the p17 matrix domain of HIV-1 was replaced with the p15 matrix and p12 domains from MMLV. Murine cells expressing this construct released significant amounts of VLPs. The construct preserved H - 2 d -restricted antigenic determinants in the remaining portion of HIV-1 Gag, allowing immunogenicity studies to be performed with mice. We demonstrated that immunizing mice with plasmid DNA or adenoviral vectors encoding this chimeric Gag did not significantly increase the HIV-1 Gag-specific cellular or humoral immune response when compared to immunization with a myristoylation-incompetent version of the construct. Thus, the release of VLPs formed in vivo may not play a major role in the immunogenicity of vectors expressing HIV-1 Gag constructs.
New agents modulating the renin-angiotensin-aldosterone system—Will there be a new therapeutic option?

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

The renin-angiotensin-aldosterone system (RAAS) is more complex than it was originally regarded. According to the current subject knowledge, there are two main axes of the RAAS: (1) angiotensin-converting enzyme (ACE)-angiotensin II-AT 1 receptor axis and (2) ACE2-angiotensin-(1-7)-Mas receptor axis. The activation of the first axis leads to deleterious effects, including vasoconstriction, endothelial dysfunction, thrombosis, inflammation, and fibrosis; therefore, blocking the components of this axis is a highly rational and commonly used therapeutic procedure. The ACE2-Ang-(1-7)-Mas receptor axis has a different role, since it often opposes the effects induced by the classical ACE-Ang II-AT 1 axis. Once the positive effects of the ACE2-Ang-(1-7)-Mas axis were discovered, the alternative ways of pharmacotherapy activating this axis of RAAS appeared. This article briefly describes new molecules affecting the RAAS, namely: recombinant human ACE2, ACE2 activators, angiotensin-(1-7) peptide and non-peptide analogs, aldosterone synthase inhibitors, and the third and fourth generation of mineralocorticoid receptor antagonists. The results of the experimental and clinical studies are encouraging, which leads us to believe that these new molecules can support the treatment of cardiovascular diseases as well as cardiometabolic disorders.
Alimentary Tract Contents of Some Biting Midge Larvae (Diptera: Ceratopogonidae)

Field: Agricultural and Biological Sciences (Life Sciences)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Lawrence J. Hribar, Gary R. Mullen; Alimentary Tract Contents of Some Biting Midge Larvae (Diptera: Ceratopogonidae). Journal of Entomological Science 1 October 1991; 26 (4): 430–435. doi: https://doi.org/10.18474/0749-8004-26.4.430 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest Search
Human Umbilical Cord Mesenchymal Stem Cells Attenuate Severe Burn-Induced Multiple Organ Injury via Potentiating IGF-1 and BCL-2/BAX Pathway

Field: Medicine (Health Sciences)

Background. Early multiple organ injuries induced by severe burn predict a high mortality. Mesenchymal stem cells (MSCs) are able to repair and reconstruct the injured tissues and organs induced by trauma and diseases. However, potential protective effect and mechanism of MSCs on multiorgan injury induced by severe burn at early stage remain to be not clarified. Therefore, this study was to explore the effect and mechanism of human umbilical cord-derived MSCs (hUCMSCs) against severe burn-induced early organ injuries in rats. Methods. Adult male Wistar rats were randomly divided into sham, burn, and burn+hUCMSCsgroups. GFP-labeled hUCMSCs or PBS was intravenous injected into respective groups. Migration and distribution patterns of GFP-labeled hUCMSCs were observed by inverted fluorescence microscope. The structures and cell apoptosis of the heart, kidney, and liver were measured by immunohistochemistry. Biochemical parameters in serum were assayed by standard Roche-Hitachi methodology. Western blotting was performed on these organs of rats in the three groups to explore the underlying mechanisms. Results. At 24 hours after hUCMSCs transplantation, we found that GFP-labeled hUCMSCs mainly localized in the blood vessel of the heart, kidney, and liver and a very few cells migrated into tissues of these organs. Compared with the sham group, structure damages and cell apoptosis of these organs were induced by severe burn, and systematic administrations of hUCMSCs significantly improved the damaged structures, cell apoptosis rates, and biochemical parameters of these organs. Furthermore, IGF-1 (insulin-like growth factor 1) level in burn+hUCMSCs group was significantly higher than that in the sham and burn groups. Meanwhile, severe burn induced BCL-2/BAX significantly decreased compared to the sham group, and it was markedly increased by hUCMSCs administration. Conclusion. The hUCMSCs transplantation can attenuate severe burn-induced early organ injuries and protect multiorgan functions by encouraging migration of hUCMSCs with blood circulation and increasing protective cytokine IGF-1 level and regulating BCL-2/BAX pathway of these vital organs. Furthermore, these data might provide the theoretical foundation for further clinical applications of hUCMSCs in burn areas.
Intradermal antitetanic-antityphoid booster by jet injection.

Field: Medicine (Health Sciences)

The immunogenicity and the reactogenicity of an unadsorbed Tetanus vaccine, intradermally administered in man as a booster immunization associated or not with lyophilized Typhoid vaccine, were comparatively studied. There were no differences in postvaccinal reactions between the Tetanus vaccine administered alone or associated with Typhoid vaccine as well as between the unadsorbed and adsorbed Tetanus vaccine. The booster inoculation carried out with Tetanus vaccine by i.d. route with doses of 10 Lf/0.1 ml proved to be effective, inducing to all the subjects a definite protective titre, maintaining for at least one year.
Developing water and sanitation services in refugee settings from emergency to sustainability – the case of Zaatari Camp in Jordan

Field: Health Professions (Health Sciences)

As of the beginning of 2017, more than four years after opening, Zaatari refugee camp in the Hashemite Kingdom of Jordan hosts around 80,000 Syrian refugees. Zaatari is one of the largest refugee camps in the world, in one of the most water scarce countries. Since its establishment, drinking water has been trucked to communal facilities. Wastewater has been trucked from these facilities and from self-constructed storages next to households. To improve future sustainability in equitable water and sanitation access, public health conditions, environmental conservation and operational costs, household connected water and sewage networks are implemented. This shift from emergency to sustainable phase benefitted from adaptation of urban infrastructure methods. Maximising such investment requires stakeholder and project management, throughout design and implementation, for quality control of all processes and outputs, asset management and administrative strategies. A shift is necessary from a humanitarian approach toward a structured master planning vision. The planning urban utility perspective is essential for ensuring operational sustainability in the conception of water and sanitation systems in Zaatari refugee camp.
The Importance of Law Practice in Achieving Legal Certainty Regarding the Grounds for Disciplinary Liability of a Judge

Field: Social Sciences (Social Sciences)

In order to overcome the relevant problem, the Law of Ukraine “On the Judiciary and the Status of Judges” significantly changed both the grounds for bringing a judge to disciplinary responsibility and the grounds for applying the most severe measure of such responsibility — dismissal of a judge. The level of detailing of such grounds is quite high, both in terms of the certainty of such grounds, and in terms of proportionality between the type of disciplinary misconduct and the type of penalty to be applied for it. At the same time, the results of the analysis of the relevant legal provisions shows that they are not devoid of evaluative, vague concepts, such as «gross disregard for the duties of a judge», «gross violation of the law», «gross negligence», «significant negative consequences». Thus, the question arises whether the problem of uncertainty of the grounds for disciplinary liability of a judge has been conceptually resolved, or whether the relevant problem continues to exist despite significant legislative detail of the relevant grounds. Resolving this issue is the purpose of this article. The existence of wording in the legislation that does not meet the requirements of legal certainty, such as «gross negligence», «gross violation of the law» carries certain risks to the independence of judges, but the existence of such risks can not be considered a reason for the law refused to use them. This is due to the objective impossibility of formulating in the law an absolutely exhaustive list of grounds for disciplinary liability in general and grounds for dismissal of a judge in particular. Critical is the issue of forming a consistent practice of the disciplinary body, which would, firstly, form the criteria for understanding vague concepts, and secondly, would not allow different approaches to responding to the same misconduct of a judge.
Race and British Colonialism in Southeast Asia, 1770-1870

Field: Social Sciences (Social Sciences)

The idea of "race" played an increasing role in nineteenth-century British colonial thought. For most of the nineteenth century, John Crawfurd towered over British colonial policy in South-East Asia, being not only a colonial administrator, journalist and professional lobbyist, but also one of the key racial theorists in the British Empire. He approached colonialism as a radical liberal, proposing universal voting for all races in British colonies and believing all races should have equal legal rights. Yet at the same time, he also believed that races represented distinct species of people, who were unrelated. This book charts the development of Crawfurd's ideas, from the brief but dramatic period of British rule in Java, to his political campaigns against James Brooke and British rule in Borneo. Central to Crawfurd's political battles were the debates he had with his contemporaries, such as Stamford Raffles and William Marsden, over the importance of race and his broader challenge to universal ideas of history, which questioned the racial unity of humanity. The book taps into little explored manuscripts, newspapers and writings to uncover the complexity of a leading nineteenth-century political and racial thinker whose actions and ideas provide a new view of British liberal, colonial and racial thought.
65. In Vivo Efficacy of Human Simulated Minocycline (MIN) against Stenotrophomonas maltophilia (STM)

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Abstract Background The current susceptibility breakpoint for MIN against STM is 4mg/L, yielding &gt;99% of isolates susceptible. Unfortunately, there are limited pre-clinical and clinical data to support this breakpoint for STM. The purpose of this study was to evaluate the efficacy of a MIN human simulated regimen (HSR) against STM across a wide range of MICs in the murine neutropenic thigh model. Methods Clinical STM with modal MIN MICS of 0.25-8mg/L were included. Confirmatory pharmacokinetic (PK) studies were performed in infected neutropenic mice to develop a MIN HSR providing an area under the curve (AUC) and maximum concentration (Cmax) exposure similar to MIN 100mg intravenous (IV) q12h at steady-state based on PK parameters from critically ill adult patients. The murine neutropenic thigh infection model was utilized to examine the antibacterial effects of the confirmed MIN HSR against 17 STM. Both thighs of neutropenic ICR mice were inoculated with bacterial suspensions of 107 colony forming units (CFU)/mL. Two hours after inoculation, the MIN HSR was administered subcutaneously (SC) over 24h. Control mice received normal saline. Efficacy was measured as the change in log10CFU/thigh at 24h compared with 0h controls. Results MIN 22, 10, 14, and 10mg/kg dosed SC at 0, 6, 12, and 18h best recapitulated the human Cmax and AUC profile. Mean ± standard deviation bacterial burden at 0h across all isolates was 6.03±0.32 log10CFU/thigh. Bacterial growth was 1.35±0.68 log10CFU/thigh in 24h controls. Six of 7 isolates (86%) with MIC ≤ 0.5mg/L achieved 1-log kill with MIN HSR (-1.44±1.37 log10CFU/thigh). All STM with MIC ≥ 1mg/L experienced bacterial growth (1.18±0.79 log10CFU/thigh) (Figure). Figure. Efficacy of a minocycline human simulated exposure of 100mg intravenous Q12h in the murine neutropenic thigh model against 17 clinical Stenotrophomonas maltophilia isolates Conclusion These data describe the in vivo efficacy of a MIN HSR with exposures similar to MIN 100mg IV q12h in critically ill adults. Lack of antibacterial activity against STM with MICs ≥ 1mg/L justifies a reassessment of the current susceptibility breakpoint. The study was funded under FDA Contract 75F40120C00171. Disclosures David P. Nicolau, PharmD, Abbvie, Cepheid, Merck, Paratek, Pfizer, Wockhardt, Shionogi, Tetraphase (Other Financial or Material Support, I have been a consultant, speakers bureau member, or have received research funding from the above listed companies.) Joseph L. Kuti, PharmD, Allergan (Speaker’s Bureau)BioMérieux (Consultant, Research Grant or Support, Speaker’s Bureau)Contrafect (Scientific Research Study Investigator)GSK (Consultant)Merck (Research Grant or Support)Paratek (Speaker’s Bureau)Roche Diagnostics (Research Grant or Support)Shionogi (Research Grant or Support)Summit (Scientific Research Study Investigator)
Komuniksasi Pelayanan Publik Kepolisian Polda Banten Melalui Program Training of Trainer (ToT)

Field: Social Sciences (Social Sciences)

The implementation of public services is an effort by the state to meet the basic needs and civil rights of every citizen to goods, services, and administrative services provided by public service providers. Likewise, the Indonesian National Police, especially the Banten Regional Police, communicates to provide public services. Through public services, the presence of state institutions can be felt by the community with various forms of communication adapted to the existence of the police itself. To provide input on how the Banten Regional Police communicates, it is necessary to make various efforts so that public services can be improved and improved. One of the methods that is trying to be done in response to findings in various studies that have been carried out related to public services in Indonesia is to carry out Community Service in the form of discussions and knowledge dissemination entitled "Communication of the Banten Regional Police through Public Service". The aim is to improve communication and improve services from various parties both at the Polda level to the Polsek level throughout the Banten region, this activity is carried out by holding a Training of Trainers (ToT) and exposure related to the importance of communication and participation of police members. Evaluation of the implementation of activities is carried out by means of lectures and discussions on materials related to communication and public services.
PIXE Real-Time Quantitative Image Projection Applied to Synchrotron XRF Imaging using the X-ray Fluorescence Microprobe

Field: Physics and Astronomy (Physical Sciences)

Journal Article PIXE Real-Time Quantitative Image Projection Applied to Synchrotron XRF Imaging using the X-ray Fluorescence Microprobe Get access C G Ryan, C G Ryan CSIRO Clayton, Australia Search for other works by this author on: Oxford Academic Google Scholar B E Etschmann, B E Etschmann CSIRO Clayton, Australia Search for other works by this author on: Oxford Academic Google Scholar S Vogt, S Vogt Argonne National Laboratory, Illinois Search for other works by this author on: Oxford Academic Google Scholar J Maser, J Maser Argonne National Laboratory, Illinois Search for other works by this author on: Oxford Academic Google Scholar C L Harland, C L Harland Australian Synchrotron Research Program, Australia Search for other works by this author on: Oxford Academic Google Scholar D Legnini D Legnini Argonne National Laboratory, Illinois Search for other works by this author on: Oxford Academic Google Scholar Microscopy and Microanalysis, Volume 11, Issue S02, 1 August 2005, Pages 428–429, https://doi.org/10.1017/S1431927605509309 Published: 01 August 2005
Nacre-Inspired Black Phosphorus/Nanofibrillar Cellulose Composite Film with Enhanced Mechanical Properties and Superior Fire Resistance

Field: Materials Science (Physical Sciences)

Natural nacre offers an optimized guiding principle for the assembly of lightweight and high-strength nanocomposites with excellent mechanical properties. Inspired by the "brick-and-mortar" layered structure of natural nacre, we present a cohort of bioinspired nanocomposites consisting of nanofibrillar cellulose (NFC) and few-layer hydroxyl functionalized black phosphorus (BP-OH) via a vacuum-assisted filtration self-assembly procedure. Owing to the well dispersed two-dimensional (2D) BP-OH in one-dimensional (1D) NFC and strong interfacial hydrogen bonding between them, these novel nacre-like BP-OHx/NFC composite films show excellent mechanical performance with tensile strength up to 214.0 MPa, 300% increase compared to pure NFC and tensile fracture strain up to 23.8%, 1.8 times higher than that of pure NFC. Moreover, these nacre-like composite films bare good fire resistance and high thermal stability. This nacre-inspired approach demonstrates a promising strategy for designing high-performance BP-OHx/NFC composite film, and the obtained bioinspired material could be a potential candidate in the application of flexible construction materials and flame retarded insulation materials.
Effect of coagulants on the physicochemical properties of fresh tofu

Field: Agricultural and Biological Sciences (Life Sciences)

Soy flour from soybean was processed to produce fresh tofu using three coagulants namely lime, Epson salt and tamarind. The results obtained showed that the pH of the samples were 7.20 for Epson salt, 5.00 for lime and 5.50 for tamarind. There were significant differences (p< 0.05) in the weight increase of all the samples with Epson salt (46.62 kg) as the highest. Epson salt had the least coagulation time of 6 min. The result of the proximate analysis shows that there were significant differences (p< 0.05) in protein, carbohydrate and moisture content in the three samples with the value of protein in Epson salt (12.40) as the highest. Further analysis revealed that there were significant differences (p<0.05) in emulsifying capacity, oil absorption capacity and swelling index in all the samples. The value of the water absorption capacity of Epson salt (4.70 g/ml) was significantly different (p< 0.05) from lime (3.80 g/ml) and tamarind (3.70 g/ml). In the mineral composition there were significant differences (p< 0.05) in Ca, Mg, K, P, Na and Fe in all the samples though K had the lowest value. There were significant differences (p<0.05) in the total fungal and viable counts. The total fungal count was least in Epson salt (2.00 x 103 CFU/g) while the total viable count was also least in Epson salt (2.6 x 103 CFU/g) after 6 days of ambient storage; however, it was highest in tamarind coagulated tofu. Epson salt coagulated fresh tofu was highly appreciated for ranking highest in protein, Ca, Mg and lowest in total fungal and viable count. Key words: Tofu, coagulants, physical characteristics, functional properties, proximate and mineral composition, microbial.
The Irrelevance of Environmental, Social, and Governance Disclosure to Retail Investors

Field: Business, Management and Accounting (Social Sciences)

Using an hourly data set on retail investor individual security positions from Robinhood Markets, we find no evidence that environmental, social, and governance (ESG) disclosures inform retail investors’ buy and sell decisions. The response on ESG press release days by retail investors is indistinguishable from nonevent days. In contrast, these same investors make economically meaningful changes to their portfolios in response to non-ESG press releases, especially those that pertain to earnings announcements. We use stock return tests to show that there is economic content in ESG press releases, and we conduct subsample analyses showing that retail investors do not respond to the most salient and economically transparent ESG disclosures. Collectively, these tests suggest that a lack of economic content, a lack of visibility, and difficulty with investment integration are unlikely to explain our findings. This paper was accepted by Suraj Srinivasan, accounting. Supplemental Material: The data files are available at https://doi.org/10.1287/mnsc.2023.4822 .
Clearing the Haze: How Does Nicotine Affect Hematopoiesis before and after Birth?

Field: Pharmacology, Toxicology and Pharmaceutics (Life Sciences)

Hematopoiesis is a tightly regulated process orchestrated by cell-intrinsic and cell-extrinsic cues. Over the past several decades, much effort has been focused on understanding how these cues regulate hematopoietic stem cell (HSC) function. Many endogenous key regulators of hematopoiesis have been identified and extensively characterized. Less is known about the mechanisms of long-term effects of environmental toxic compounds on hematopoietic stem and progenitor cells (HSPCs) and their mature immune cell progeny. Research over the past several decades has demonstrated that tobacco products are extremely toxic and pose huge risks to human health by causing diseases like cancer, respiratory illnesses, strokes, and more. Recently, electronic cigarettes have been promoted as a safer alternative to traditional tobacco products and have become increasingly popular among younger generations. Nicotine, the highly toxic compound found in many traditional tobacco products, is also found in most electronic cigarettes, calling into question their purported “safety”. Although it is known that nicotine is toxic, the pathophysiology of disease in exposed people remains under investigation. One plausible contributor to altered disease susceptibility is altered hematopoiesis and associated immune dysfunction. In this review, we focus on research that has addressed how HSCs and mature blood cells respond to nicotine, as well as identify remaining questions.