MP60-05 5-HT IN THE RAT PREFRONTAL CORTEX CONTROLS THE MICTURITION REFLEX VIA 5-HT2A AND 5-HT7.

Field: Neuroscience (Life Sciences)

You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology I1 Apr 2016MP60-05 5-HT IN THE RAT PREFRONTAL CORTEX CONTROLS THE MICTURITION REFLEX VIA 5-HT2A AND 5-HT7. Hiroki Chiba, Takahiko Mitsui, Takeya Kitta, Yu Ohmura, Kimihiko Moriya, Yukiko Kanno, Mitsuhiro Yoshioka, and Nobuo Shinohara Hiroki ChibaHiroki Chiba More articles by this author , Takahiko MitsuiTakahiko Mitsui More articles by this author , Takeya KittaTakeya Kitta More articles by this author , Yu OhmuraYu Ohmura More articles by this author , Kimihiko MoriyaKimihiko Moriya More articles by this author , Yukiko KannoYukiko Kanno More articles by this author , Mitsuhiro YoshiokaMitsuhiro Yoshioka More articles by this author , and Nobuo ShinoharaNobuo Shinohara More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.858AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The prefrontal cortex (PFC) is the critical region for neural control of the micturition reflex. Many functional imaging studies, such as f-MRI, have been reported in human. We reported the role of serotonergic mechanism in the rat PFC for controlling the micturition reflex with using an in vivo microdialysis study. To evaluate more function of 5-HT in the control of the micturition reflex, we used the microinjection method. METHODS Adult female SD rats were used. Following anesthesia with urethane, guide cannulas and a polyethylene catheter were implanted into the PFC and the bladder, respectively. All experiments were conducted 7 days after the surgery under consciousness and free movement. Infusing the saline into the bladder for 60 minutes, several micturition reflexes were observed. After that, 5-HT antagonists (5-HT1A, 5-HT2A, 5-HT2C, 5-HT3, 5-HT4, 5-HT7) were injected into the bilateral PFC, and infused the saline into the bladder for 60 min. Intercontraction interval (ICI) and maximum voiding pressure (MVP) were measured before and after the injection. PFC was divided into the two regions, prelimbic region (PL) and infralimbic region (IL) (Fig.1). RESULTS ICI was significantly increased after the injection of 5-HT2A antagonist (MDL11939, 300ng/side) into the PL region (n=6, p<0.05). In contrast, ICI was significantly decreased after injection of 5-HT7 antagonist (SB266970, 1µg/side) into the PL (n=9, p<0.05) (Fig.2). No change was found in the IL region. MVP was not changed after injection of all 5-HT antagonists. CONCLUSIONS In the rat PFC, especially PL region, 5-HT accelerates the micturition reflex via 5-HT2A, and suppresses it via 5-HT7 (Fig.3). © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e794-e795 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Hiroki Chiba More articles by this author Takahiko Mitsui More articles by this author Takeya Kitta More articles by this author Yu Ohmura More articles by this author Kimihiko Moriya More articles by this author Yukiko Kanno More articles by this author Mitsuhiro Yoshioka More articles by this author Nobuo Shinohara More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...