First Report of Tomato spotted wilt virus Infecting Peperomia obtusifolia in South Korea

Field: Agricultural and Biological Sciences (Life Sciences)

HomePlant DiseaseVol. 103, No. 3First Report of Tomato spotted wilt virus Infecting Peperomia obtusifolia in South Korea PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Tomato spotted wilt virus Infecting Peperomia obtusifolia in South KoreaJ. Y. Yoon, G. S. Choi, S. J. Kwon, and I. S. ChoJ. Y. Yoon†Corresponding author: J. Y. Yoon; E-mail: E-mail Address: juyeon74@gmail.comhttp://orcid.org/0000-0003-1646-7310Virology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, 55365, Republic of Korea, G. S. ChoiVirology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, 55365, Republic of Korea, S. J. Kwonhttp://orcid.org/0000-0002-7127-3634Virology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, 55365, Republic of Korea, and I. S. ChoVirology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, 55365, Republic of KoreaAffiliationsAuthors and Affiliations J. Y. Yoon † G. S. Choi S. J. Kwon I. S. Cho Virology Unit, Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju, 55365, Republic of Korea Published Online:21 Jan 2019https://doi.org/10.1094/PDIS-07-18-1209-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Peperomia obtusifolia (called baby rubberplant or pepper face) is a species of flowering plant and one of the popular indoor ornamental plants. P. obtusifolia in South Korea was imported from Taiwan and China beginning in 1970. In July 2018, virus-like symptoms including mottle and yellowing followed by vein clearing on leaves were observed in P. obtusifolia (cv. Shinholly) plants in a greenhouse in Geyongnam province, South Korea. Approximately 20% of peperomia plants (from a total of 3,000 plants) in the greenhouse showed virus-like foliar symptoms. To identify the causal virus, four symptomatic leaf samples of P. obtusifolia plants were analyzed by transmission electron microscopy (TEM) of leaf dip preparations. Tospovirus-like particles (about 80 to 100 nm in diameter) were observed for all the samples. To confirm the TEM result, single leaves from 20 symptomatic P. obtusifolia plants were further analyzed using double-antibody sandwich (DAS)-ELISA kits (Agdia, Elkhart, IN) for the presence of three peperomia-infecting viruses (cucumber mosaic virus, impatiens necrotic spot virus, and tomato spotted wilt virus [TSWV]), as reported previously (Corbett 1956; Milojević et al. 2013). DAS-ELISA showed that TSWV was present in all tested naturally infected P. obtusifolia plants. For the other two viruses all samples tested negative by DAS-ELISA. TSWV is currently widespread in South Korea, infecting pepper, tomato, potato, ornamental plants, and wild plant species (Choi et al. 2014; Yoon et al. 2018). The single infection of TSWV in symptomatic P. obtusifolia plants was further confirmed by a TSWV-ImmunoStrip kit (Agdia). To confirm the presence of TSWV alone, reverse transcription polymerase chain reaction products were synthesized for L, M, and S RNA segments using Tospovirus-specific primers and TSWV-specific primers (Batuman et al. 2014; Choi et al. 2014). The expected fragments of 446, 403, and 777 bp were amplified and sequenced (LC191861, LC191862, and LC167301). The sequences of each fragment were identical to the previously described (Batuman et al. 2014) consensus sequences, showing 99% identities with TSWV-L, M, and S RNA (KP008130, FM163373, and FR693078). These results clearly showed that the results of DAS-ELISA and the TSWV-ImmunoStrip kit were not owing to interspecies cross-reactivity of the virus-specific antibodies. The results of sequence comparisons showed no evidence of reassortment between TSWV and another tospovirus. Taken together, the TSWV-peperomia isolate is demonstrated to be the causal agent in the diseased P. obtusifolia plants. The data suggest that TSWV can be detrimental to production of P. obtusifolia in South Korea. Therefore, effective management strategies should be taken to minimize the impact of this virus on P. obtusifolia. To our knowledge, this is the first report of TSWV in P. obtusifolia in South Korea.References:Batuman, O., et al. 2014. Plant Dis. 98:286. https://doi.org/10.1094/PDIS-07-13-0685-PDN Link, ISI, Google ScholarChoi, S. K., et al. 2014. Plant Dis. 98:1283. https://doi.org/10.1094/PDIS-02-14-0173-PDN Abstract, Google ScholarCorbett, M. K. 1956. Proc. Fla. State Hort. Soc. 69:357. Google ScholarMilojević, K., et al. 2013. Plant Dis. 97:1004. https://doi.org/10.1094/PDIS-01-13-0089-PDN Link, ISI, Google ScholarYoon, J. Y., et al. 2018. Plant Dis. 102:690. https://doi.org/10.1094/PDIS-07-17-1091-PDN Link, ISI, Google ScholarFunding: Funding was provided by Rural Development Administration (grant no. PJ01359101).DetailsFiguresLiterature CitedRelated Vol. 103, No. 3 March 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 6 Mar 2019Published: 21 Jan 2019First Look: 17 Oct 2018Accepted: 8 Oct 2018 Pages: 593-593 Information© 2019 The American Phytopathological SocietyFundingRural Development AdministrationGrant/Award Number: PJ01359101Cited byElectron microscopic methods for virus diagnosisFirst Report of Tomato Spotted Wilt Virus in Oxypetalum coeruleum in KoreaResearch in Plant Disease, Vol. 28, No. 4Tomato spotted wilt orthotospovirus (tomato spotted wilt)CABI Compendium, Vol. CABI CompendiumFirst Report of a Resistance-Breaking Strain of Tomato Spotted Wilt Orthotospovirus Infecting Capsicum annuum Carrying the Tsw Resistance Gene in South KoreaJ. Y. Yoon, N. H. Her, I. S. Cho, B. N. 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