jimnoneill/paper-to-field-training huggingface dataset:
Nutritional Quality Improvement Of Soybean Meal By Bacillus Velezensis And Lactobacillus Plantarum During Two-Stage Solid- State Fermentation

Field: Agricultural and Biological Sciences (Life Sciences)

Abstract Bacillus velezensis is widely used for agricultural biocontrol, due to its ability to enhance plant growth while suppressing the growth of microbial pathogens. However, there are few reports on its application in fermented feed. Here, a two-stage solid-state fermentation process using Bacillus velezensis followed by Lactobacillus plantarum was developed to degrade antinutritional factors (ANFs) and improve soybean meal (SBM) nutrition for animal feed. The process was evaluated for performance in degrading SBM antinutritional factors, dynamic changes in physicochemical characteristics, microorganisms and metabolites. After two-stage fermentation, degradation rates of glycinin and β-conglycinin contents reached 78.60% and 72.89%, respectively. The pH of fermented SBM (FSBM) decreased to 4.78±0.04 and lactic acid content reached 183.38±4.86 mmol/kg. NSP-degrading enzymes (Non-starch polysaccharide, NSPases) and protease were detected from the fermented product, which caused the changed microstructure of SBM. Compared to uninoculated SBM, FSBM exhibited increased proportions of crude protein (51.97±0.44% vs. 47.28±0.34%), Ca, total phosphorus (P), and trichloroacetic acid-soluble protein (11.79±0.13% vs. 5.07±0.06%). Additionally, cellulose and hemicellulose proportions declined by 22.10% and 39.15%, respectively. Total amino acid content increased by 5.05%，while the difference of AA content between the 24h, 48 h and 72 h of fermentation was not significant ( P ＞0.05). Furthermore, FSBM also showed antibacterial activity against Staphylococcus aureus and Escherichia coli . These results demonstrated that two-stage SBM fermentation process based on Bacillus velezensis 157 and Lactobacillus plantarum BLCC2-0015 is an effective approach to reduce ANFs content and improve the quality of SBM feed.
Pembiasaan Berwirausaha Untuk Seluruh Warga SMA N 1 Karangnunggal Melalui Aplikasi E Commerce SOS (SMANKA Online Shop)

Field: Business, Management and Accounting (Social Sciences)

GoEntrepreneurship is the attitude, soul and ability to create something new that is valuable and useful to yourself and others. Entrepreneurship is a mindset and spirit that is always positive or creative, empowering, creative, creative, humble, and seeks to increase income through business. Entrepreneurship occurs when someone tries to develop a new business or idea. Entrepreneurs are always dissatisfied with their performance. An entrepreneur is someone who can seize the opportunity to develop a business in order to improve the standard of living. Against this background, the author believes that entrepreneurship should be cultivated in schools with the aim of making it a habit, so that young entrepreneurs grow from an early age. The problem in this case study is the impact of e-commerce SOS (SMANKA online shop) on increasing entrepreneurship in schools. Based on our findings, we are very satisfied with the results, more than 1000 users, 512 partners, and 600 transactions registered on E-commerce SOS within 100 days of using e-commerce SOS. Students feel an advantage in personal character/ethics, online sales procedures, and store setup to attract buyers. This study is therefore of great value in preparing students for the future
Chromosome analysis in chorionic villi samples from the first trimester elective terminations.

Field: Medicine (Health Sciences)

Chorionic villi sampling (CVS) has become a first trimester alternative to amniocentesis for prenatal diagnosis. The cytogenetic findings in 150 experimental samples are presented. The ages of the mothers ranged from 12 to 35 years, but the majority of them were 18 and 19 years of age. Various parameters of culturing and processing the samples in order to improve the method, were investigated. Short term incubation for 48 h was the method routinely employed in processing the biopsies for cytogenetic analysis. In the first series of 100 cases one mosaic case (46,XX/45,X), one Robertsonian translocation (13;14), one marker chromosome and one fragment were found. The foetal tissues were not analysed for chromosomes. In the second series of 50 samples, one case of mosaicism was found in the chorionic villi (46,XX/47,XX, 18q-), but this abnormality was absent in the foetal tissue. One variant inv(9) was observed in the foetal tissue as well as in the chorionic villi. In all other cases the karyotypes from the chorionic villi samples matched those of the corresponding foetal samples. There was no maternal contamination in this series of 50 samples. The discrepancies in the cytogenetic results from other investigators are discussed.
Persepsi Masyarakat Madani Terhadap Asuransi Syariah

Field: Social Sciences (Social Sciences)

The rapid development of sharia insurance in Indonesia is an achievement for Muslims as well as representing significant economic growth for the people. But on the other hand, it cannot be denied that there are still many obstacles to be faced, especially with regulations and public perceptions of the sharia system. This study aims to obtain information regarding the perceptions of Serang City civil society towards sharia insurance and is presented descriptively in the form of a narrative. The phenomenological approach aims to explain a phenomenon or the reality of something that describes the characteristics of the subject under study. In this study in general put forward a good response to sharia insurance. Whether it's the people of Serang City who have been members of conventional insurance, or who have never joined as members of any insurance but already know and understand the insurance scheme.
High-throughput microarray technology in diagnostics of enterobacteria based on genome-wide probe selection and regression analysis

Field: Immunology and Microbiology (Life Sciences)

Background: The Enterobacteriaceae comprise a large number of clinically relevant species with several individual subspecies. Overlapping virulence-associated gene pools and the high overall genome plasticity often interferes with correct enterobacterial strain typing and risk assessment. Array technology offers a fast, reproducible and standardisable means for bacterial typing and thus provides many advantages for bacterial diagnostics, risk assessment and surveillance. The development of highly discriminative broad-range microbial diagnostic microarrays remains a challenge, because of marked genome plasticity of many bacterial pathogens. Results: We developed a DNA microarray for strain typing and detection of major antimicrobial resistance genes of clinically relevant enterobacteria. For this purpose, we applied a global genome-wide probe selection strategy on 32 available complete enterobacterial genomes combined with a regression model for pathogen classification. The discriminative power of the probe set was further tested in silico on 15 additional complete enterobacterial genome sequences. DNA microarrays based on the selected probes were used to type 92 clinical enterobacterial isolates. Phenotypic tests confirmed the array-based typing results and corroborate that the selected probes allowed correct typing and prediction of major antibiotic resistances of clinically relevant Enterobacteriaceae, including the subspecies level, e.g. the reliable distinction of different E. coli pathotypes. Conclusions: Our results demonstrate that the global probe selection approach based on longest common factor statistics as well as the design of a DNA microarray with a restricted set of discriminative probes enables robust discrimination of different enterobacterial variants and represents a proof of concept that can be adopted for diagnostics of a wide range of microbial pathogens. Our approach circumvents misclassifications arising from the application of virulence markers, which are highly affected by horizontal gene transfer. Moreover, a broad range of pathogens have been covered by an efficient probe set size enabling the design of high-throughput diagnostics.
Head Impact Situations in Professional Football (Soccer)

Field: Medicine (Health Sciences)

Abstract To assess head impact incidents (HIIs) and to distinguish diagnosed head injuries from other incidents, a video observation analysis of match HIIs was conducted in the German Bundesliga (2017/18 season). Video recordings of each match were screened to identify the respective events. Head injury data were identified by a prospective injury registry. HII and head injury incidence rates (IR) were calculated with 95% CIs. The total number of HIIs was 1,362 corresponding to an IR of 134.9/1000 match hours (95% CI 127.9–142.2). In 123 HII (IR 12.2, 95% CI 10.2–14.5) the contact was classified as severe. Head contact with the opponent was the most frequent cause (85%). The most frequent mechanism was in 44% (combined) the arm and elbow-to-head, followed by head-to-head and hand-to-head contacts (each 13%). In 58%, the HIIs occurred during header duels. Twenty-nine head injuries were recorded (IR 2.9, 95% CI 2.0–4.1). Concussions/traumatic brain injuries accounted for 48%, head/facial fractures 24%, head/facial contusions 21%, and lacerations/abrasions 7%. The number of HIIs not classified as concussions/more severe trauma was high. Identification of HIIs and head injury severity should be improved during on-field assessment as many head injuries might go unrecognised based on the large number of HIIs.
Network structure in poly(vinylidene fluoride–trifluoroethylene) electrostrictive films

Field: Materials Science (Physical Sciences)

Recent studies of electron-irradiated copolymers of vinylidene fluoride and trifluoroethylene have demonstrated the potential for achieving a large electrostrictive response in these materials. Since the principal effect of the radiation is network formation, alternative methods of achieving improved electromechanical properties are available. In this letter, we use a two-solvent swelling technique to quantify the polymer-solvent interaction parameter for the copolymer networks. This enables determination of their crosslink densities, along with a measure of the degree of degradation accompanying radiolysis. Copolymer networks were then prepared chemically using organic peroxide. These networks were found to be similar to the radiation-crosslinked materials, both in the extent of degradation and with respect to the Curie temperature and crystalline melting behavior. The latter are crucial to the development of better electrostrictive properties.
SH1089777.09FU

Field: Agricultural and Biological Sciences (Life Sciences)

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.
Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia

Field: Medicine (Health Sciences)

Dysregulation of kinase signaling pathways favors tumor cell survival and therapy resistance in cancer. Here, we reveal a posttranslational regulation of kinase signaling and nuclear receptor activity via deubiquitination in T cell acute lymphoblastic leukemia (T-ALL). We observed that the ubiquitin-specific protease 11 (USP11) is highly expressed and associates with poor prognosis in T-ALL. USP11 ablation inhibits leukemia progression in vivo, sparing normal hematopoiesis. USP11 forms a complex with USP7 to deubiquitinate the oncogenic lymphocyte cell–specific protein-tyrosine kinase (LCK) and enhance its activity. Impairment of LCK activity leads to increased glucocorticoid receptor (GR) expression and glucocorticoids sensitivity. Genetic knockout of USP7 improved the antileukemic efficacy of glucocorticoids in vivo. The transcriptional activation of GR target genes is orchestrated by the deubiquitinase activity and mediated via an increase in enhancer-promoter interaction intensity. Our data unveil how dysregulated deubiquitination controls leukemia survival and drug resistance, suggesting previously unidentified therapeutic combinations toward targeting leukemia.
Research on post-bidding bundle strategy for online reverse auction

Field: Decision Sciences (Social Sciences)

Currently online reverse auction (e-RA) is often used in procurement. However, winner determination problem (WDP) is NP-hard problem and practical use is difficult. As a research focus, `bundling' is an effective way to cut down the complexity of WDP for e-RA, and often applied in practice. Although there are some qualitative researches in this field, quantitative bundling is still a problem. This paper designs post-bidding bundle strategy (PBBS) for e-RA to focus on this issue. Based on mathematical model and the bidding data from suppliers, this strategy uses `item similarity' and `market competitiveness' to measure the possibility of two items in the same bundle. Specially, item similarity measure refers to two classic methods in scientometrics: Salton and Jaccard. Finally, as shown with the 7 th pharmaceutical centralized purchasing bidding data in china, this strategy of bundling has good theoretical value and practical significance in quantitative bundling.
Design and implementation of synchronization control system for multi-break vacuum circuit breaker

Field: Engineering (Physical Sciences)

A synchronization control system is designed and implemented for multi-break vacuum circuit breaker,which takes DSP(Digital Signal Processor) and FPGA(Field Programmable Gate Array) as its hardware core and adopts the hardware architecture combining bus backplane and function flapper.Its software contains a zero voltage / current detection algorithm and the protocol designed for the fiber communication between system and breaker,which improves the synchronization control algorithm.Results of electromagnetic compatibility test and synchronous breaking experiments show that,the designed system has good electromagnetic compatibility,meets the requirements of multi-break vacuum circuit breaker for real-time signal processing,and realizes the synchronous operation of multi-break vacuum circuit breaker perfectly.
USING COALESCENT SIMULATIONS TO TEST THE IMPACT OF QUATERNARY CLIMATE CYCLES ON DIVERGENCE IN AN ALPINE PLANT-INSECT ASSOCIATION

Field: Agricultural and Biological Sciences (Life Sciences)

The Quaternary climate cycles forced species to repeatedly migrate across a continually changing landscape. How these shifts in distribution impacted the evolution of unrelated but ecologically associated taxa has remained elusive due to the stochastic nature of the evolutionary process and variation in species-specific biological characteristics and environmental constraints. To account for the uncertainty in genealogical estimates, we adopted a coalescent approach for testing hypotheses of population divergence in coevolving taxa. We compared genealogies of a specialized herbivorous insect, Parnassius smintheus (Papilionidae), and its host plant, Sedum lanceolatum (Crassulaceae), from the alpine tundra of the Rocky Mountains to null distributions from coalescent simulations to test whether tightly associated taxa shared a common response to the paleoclimatic cycles. Explicit phylogeographic models were generated from geologic and biogeographic data and evaluated over a wide range of divergence times given calibrated mutation rates for both species. Our analyses suggest that the insect and its host plant responded similarly but independently to the climate cycles. By promoting habitat expansion and mixing among alpine populations, glacial periods repeatedly reset the distributions of genetic variation in each species and inhibited continual codivergence among pairs of interacting species.
IT-enabled organizational transformation: a structured literature review

Field: Computer Science (Physical Sciences)

Purpose This paper presents a structured literature review of studies on IT-enabled organizational transformation to determine the state of the art and to identify areas for future research. Design/methodology/approach We collect 201 academic publications on IT-enabled organizational transformation and analyze them from three perspectives: a publication perspective, a research perspective and a conceptual perspective. Findings From a publication perspective, we identify and synthesize the seminal works to provide a brief history of research on IT-enabled organizational transformation. From a research perspective, we show that studies in this area have seldom been grounded in theory and have predominantly used qualitative approaches, while only a few studies have drawn from quantitative data. From a conceptual perspective, we show that most research has studied higher levels of transformation, especially process redesign. Originality/value This review presents the landscape of the literature on IT-enabled organizational transformation, which provides a foundation for future research.
In vivo molecular imaging of cancer stem cells in mice with xenografted colon cancer

Field: Medicine (Health Sciences)

1119 Objectives To monitor the behavior of cancer stem cell (CSC) in vivo, we characterized human colon cancer cell line Caco2 according to CD133 and CD44, putative CSC surface markers, and then established an in vivo molecular imaging method in a xenografted mouse model. Methods We characterized Caco2 cells by the expression of CD133/CD44 marker using fluorescence activated cell sorting analysis according to the cell passage. By means of transfection of Caco2 cells with an enhanced firefly luciferase (effLuc) retroviral vector, we established a stable cell line (Caco2-effLuc). After separation using automated magnetic cell sorter, both CD133+ and CD133- groups were cultured in a 24-well plate and injected subcutaneously into nude mice with/without Matrigel, and then they were monitored using a luminometer for in vitro and optical imaging system for in vivo. Results Early passage Caco2 cells expressed CD133/CD44 (passage 15: 58%/10%) highly, but dramatically decreased in the late passage (passage 25; 5.4%/1.1%). With assistance of Matrigel, both CD133+ and CD133- cells could make a xenograft, but the proliferation of the CD133+ cells were greater than that of CD133- cells, approximately 80 folds at 10 days after the injection of the cells. In contrast to CD133- cells that were non-capable of forming a xenograft without Matrigel, CD133+ cells could make a xenograft even without Matrigel though the proliferation was lagged. Conclusions The putative CSC marker was highly expressed in the early passage Caco2 cell line. Colon cancer cells expressing CD133 might play a key role in tumor proliferation
Motion simulator study of longitudinal stability requirements for large delta wing transport airplanes during approach and landing with stability augmentation systems failed

Field: Engineering (Physical Sciences)

A ground-based simulator investigation was conducted in preparation for and correlation with an-flight simulator program. The objective of these studies was to define minimum acceptable levels of static longitudinal stability for landing approach following stability augmentation systems failures. The airworthiness authorities are presently attempting to establish the requirements for civil transports with only the backup flight control system operating. Using a baseline configuration representative of a large delta wing transport, 20 different configurations, many representing negative static margins, were assessed by three research test pilots in 33 hours of piloted operation. Verification of the baseline model to be used in the TIFS experiment was provided by computed and piloted comparisons with a well-validated reference airplane simulation. Pilot comments and ratings are included, as well as preliminary tracking performance and workload data.
The rate of mutation of L5178Y asparagine-dependent mouse leukemia cells to asparagine independence and its biological consequences.

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Summary L5178Y mouse lymphoblastic leukemia cells normally require asparagine (ASN) for growth, but phenotypically stable ASN-independent cells are detectable at low frequency by direct cloning, in ASN-free soft-agar medium, of cells from cultures that have been grown nonselectively in medium containing ASN. The rate of mutation in vitro to ASN independence was determined by the Luria-Delbruck fluctuation test to be approximately 9 × 10 -7 /cell/generation. This test also demonstrated that the conversion from ASN dependence to independence has the properties of a spontaneous and random mutational event. New ASN-independent mutants isolated in this way and maintained in ASN-containing medium have significant levels of ASN synthesis but are slightly stimulated by ASN when subcultured in ASN-free medium. However, further selection by growth of these mutant cells in ASN-free medium gives cell lines that grow identically with or without ASN and produce ASN in excess of their demands for growth. A substrain of L5178Y cells resistant to arabinosylcytosine had a lower rate of mutation to ASN independence in vitro . The mutation to ASN independence also occurs in vivo in mice with L5178Y ascites tumors. The frequency of ASN independent cells observed in vivo with L5178Y cells is highly dependent on the regimen of tumor transfer and is a determinant of the effectiveness of therapy with l-asparaginase in this model tumor system.
Neuregulin modulates hormone receptor levels in breast cancer through concerted action on multiple signaling pathways

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Abstract The Neuregulins (NRGs) are growth factors that bind and activate ErbB/HER receptor tyrosine kinases. Some reports have described an interplay between this ligand–receptor system and hormonal receptors in breast cancer. However, the mechanisms by which NRGs regulate hormonal receptor signaling have not been sufficiently described. Here, we show that in breast cancer cells the activation of NRG receptors down-regulated ERα through a double mechanism that included post-transcriptional and transcriptional effects. This regulation required the concerted participation of three signaling routes: the PI3K/AKT/mTOR, ERK1/2, and ERK5 pathways. Moreover, these three routes were also involved in the phosphorylation of ERα at serines 118 and 167, two residues implicated in resistance to endocrine therapies. On the other hand, NRGs conferred resistance to fulvestrant in breast cancer cells and this resistance could be reversed when the three pathways activated by NRGs were simultaneously inhibited. Our results indicate that estrogen receptor-positive (ER+) breast tumors that can have access to NRGs may be resistant to fulvestrant. This resistance could be overcome if strategies to target the three main pathways involved in the interplay between NRG receptors and ERα could be developed.