jimnoneill/paper-to-field-training huggingface dataset:
Data from Transmembrane and Coiled-Coil Domain 1 Impairs the AKT Signaling Pathway in Urinary Bladder Urothelial Carcinoma: A Characterization of a Tumor Suppressor

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

<div>Abstract<p><b>Purpose:</b> Urinary bladder urothelial carcinoma (UBUC) is a common malignant disease in developed countries. Cell-cycle dysregulation resulting in uncontrolled cell proliferation has been associated with UBUC development. This study aimed to explore the roles of <i>TMCO1</i> in UBUCs.</p><p><b>Experimental Design:</b> Data mining, branched DNA assay, immunohistochemistry, xenograft, cell culture, quantitative RT-PCR, immunoblotting, stable and transient transfection, lentivirus production and stable knockdown, cell-cycle, cell viability and proliferation, soft-agar, wound-healing, transwell migration and invasion, coimmunoprecipitation, immunocytochemistry, and AKT serine/threonine kinase (AKT) activity assays and site-directed mutagenesis were used to study <i>TMCO1</i> involvement <i>in vivo</i> and <i>in vitro</i>.</p><p><b>Results:</b> Data mining identified that the <i>TMCO1</i> transcript was downregulated during the progression of UBUCs. In distinct UBUC-derived cell lines, changes in TMCO1 levels altered the cell-cycle distribution, cell viability, cell proliferation, and colony formation and modulated the AKT pathway. TMCO1 recruited the PH domain and leucine-rich repeat protein phosphatase 2 (PHLPP2) to dephosphorylate pAKT1(serine 473) (S473). Mutagenesis at S60 of the TMCO1 protein released TMCO1-induced cell-cycle arrest and restored the AKT pathway in BFTC905 cells. Stable <i>TMCO1</i> (wild-type) overexpression suppressed, whereas T33A and S60A mutants recovered, tumor size in xenograft mice.</p><p><b>Conclusions:</b> Clinical associations, xenograft mice, and <i>in vitro</i> indications provide solid evidence that the <i>TMCO1</i> gene is a novel tumor suppressor in UBUCs. TMCO1 dysregulates cell-cycle progression via suppression of the AKT pathway, and S60 of the TMCO1 protein is crucial for its tumor-suppressor roles. <i>Clin Cancer Res; 23(24); 7650–63. ©2017 AACR</i>.</p></div>
Analysis of Tourism Potential and Marketing Integration in Mundeh Kangin Village

Field: Social Sciences (Social Sciences)

The purpose of this research is to determine the tourism potential of Mundeh Kangin village, and howmarketing integration is carried out in Mundeh Kangin village, so that a suitable strategy can be implementedin the future. This research uses a qualitative descriptive approach with data collection techniques in the formof direct observation, interviews and document study. In-depth interviews with informants, namely the headof Mundeh Kangin Village, the tourism awareness group and the Farmer's group of Mundeh Kangin village. Thescope of the problem discussed in this research is regarding the potential and marketing mix of Mundeh KanginVillage as a pioneering tourism village in Tabanan Regency. Based on the results of internal, external andinteractive marketing research in Mundeh Kangin village, Tabanan, several key points can be concluded namelyLocal product potential, External communication challenges, and Role of Interactive marketing has beenidentified as a potential strategy to overcome challenges in external marketing. By involving the communitydirectly through social media, community events, interactive content, and collaboration with local influencers,interactive marketing is able to increase engagement and awareness about local products in Mundeh KanginTabanan village.Keywords: Tourism potential, integration, marketing
Determinant of Audit Report Lag Among Mining Companies In Indonesia

Field: Business, Management and Accounting (Social Sciences)

Time period in completing the audit work until the date of publishing audit report is called audit report lag. BAPEPAM requires each of going-public companies to publish their annual reports not later than three months after the fiscal year ends. The aim of this research was to determine the effect of profitability, solvency, company size, audit opinion, and size of public accounting firm on audit report lag at mining companies listed on Indonesia Stock Exchange during the period of 2013-2017. As many as 12 samples were obtained through purposive sampling technique. The data analysis technique used was the multiple regression analysis. The results showed that the profitability and company size negatively affected the audit report lag, while the other variables, such as solvency, audit opinion, and size of public accounting firm, had no significant effect on the audit report. The result of simultaneous test showed that all independent variables influenced audit report lag with 32.8% of determination coefficient.
Effects on The Production Performance and Egg Quality under The Supplement of Cuttlebone in Feedstuff

Field: Agricultural and Biological Sciences (Life Sciences)

Three hundred and fifte en 14 - week hyline browns were used to investigate the effects of cuttlebone supplied in feedstuff on production performance and egg quality. Hyline browns were randomly divided into 7 groups, each group was assigned 3 replications and 15 hyline browns per replication. The results showed that the egg production rate, average egg weight every day, weight of every egg and eggshell thickness were inceased significantly (P 0.05) under supplying cuttlebone in feedstuff. They averagely enhanced 13.0%, 5.7g, 8.4g and 0.05mm than that of contral, respectively. The ratio of feed to egg and broken egg rate significantly decreased when cuttlebone was supplied to feedstuff, which reduced to 31.2% and 13.7% than that of control, respectively (P 0.05). No distincted effects on the weight of egg shell , egg yolk and egg albumen were observed in this study. Analysis showed that 1.5g-2.0g cuttlebone was supplied to feedstuff every day for150 hyline browns increased general production performance significantly.
Optical imaging of intrinsic signals in the auditory cortex

Field: Neuroscience (Life Sciences)

The optical imaging of intrinsic signals for exploring central auditory activity patterns was investigated on acoustically evoked intrinsic signals from the auditory cortex of anesthetized adult chinchillas. Intrinsic signals in response to acoustic stimulation were found in a cortical area previously defined as primary auditory cortex. The stimulus evoked intrinsic signal has an onset 0.5-1 s after stimulus onset, reaches a maximum after 3-4 s, and then decays at a slower rate. Changes in light absorption in three regions within the activated auditory cortex are presented against activity in two control areas: a nonauditory cortical area and the bone on the edge of the craniotomy.
Potential Benefits of Navy Dive Computer Use in Ships Husbandry Diving: Analysis of Dives Conducted at Puget Sound Naval Shipyard

Field: Health Professions (Health Sciences)

Generation III Air Navy Dive Computers (AIR III) were used to record 315 ships husbandry dives conducted by divers breathing air at the Puget Sound Naval Shipyard from November 2007 to January 2008.For each profile, decompression status was assessed from the air tables in Rev. 6 of the U.S. Navy Diving Manual and from the AIR Ills, thirty-five dives were repetitive dives (within 12 hours of a previous exposure), so 280 are distinct profiles for analysis.According to probabilistic models, the average predicted risk of decompression sickness for these profiles was low (<1 %), as expected.The majority of dives ended because the assigned work was completed, not because the no-stop limits for the maximum depth were reached.Two 34 fsw profiles were of moderate risk when evaluated by both USN93 and BVM-3 These two profiles exceeded the Rev. 6 U.S. Navy Diving Manual no-stop limits, and one of them required five minutes of decompression per the AIR III record.More than 10% of the dives were conducted at or beyond the no-stop limits for Rev. 6 of the Diving Manual; divers would have directly benefited from using AIR Ills on these dives.The AIR Ills will also benefit these divers in cases where a diver needs to make a downward excursion (tool recovery, etc.), but until divers are allowed to dive per the guidance of the AIR Ills, we will be unable to fully tabulate the benefits.A future AIR III benefit for divers will be a reduction in data entry when the Navy Dive computer profiles are downloaded into the Dive Recording System, an expected by-product from development of the Topside Decompression Monitor.
Jejunal angiodysplasia: surgery can be life-saving – a case report

Field: Medicine (Health Sciences)

Introduction: Angiodysplasia, a rare cause of gastrointestinal (GI) bleeding, presents a spectrum of clinical manifestations from anemia to life-threatening hemorrhage. This case study emphasizes the significance of considering intestinal vascular malformations as a differential diagnosis, especially in the context of chronic anemia and GI bleeding. Jejunal angiodysplasia, though infrequent, poses diagnostic challenges due to the hidden nature of the small bowel in the GI system. Case presentation: A 23-year-old male presented with acute hematochezia and melena, necessitating prompt intervention. Despite a normal esophagogastroduodenoscopy, colonoscopy was hindered, CT angiography could not be performed due to the patient’s unstable condition, leading to a planned exploratory laparotomy. Surgical exploration revealed a mucosal vascular lesion in the jejunum, prompting resection, and anastomosis. The patient’s postoperative course was uneventful, reinforcing the importance of swift diagnosis and intervention. Clinical discussion: Angiodysplasia’s pathogenesis remains unclear, with hypotheses implicating vascular endothelial growth factor and submucosal changes. Challenges in management revolve around lesion localization and stabilizing hemodynamics, necessitating a multidisciplinary approach. While endoscopy is often diagnostic and therapeutic, advanced modalities such as CT angiography may be required. Literature review highlights diverse presentations and successful interventions, including embolization and surgical resection. Conclusion: Jejunal angiodysplasia demands a comprehensive diagnostic and therapeutic strategy. The presented case underscores the pivotal role of endoscopy, embolization, and surgery in managing this condition. Timely diagnosis and intervention are crucial for mitigating the impact of angiodysplasia, necessitating further research and collaborative efforts for improved management of this rare condition.
Reduced-order robust compensator design with real parameter uncertainty

Field: Engineering (Physical Sciences)

A method based on observer theory is presented for the construction of design equations for low-order controllers to provide H∞ norm constraint for systems with real-parameter uncertainties. The multi-goal robust stability and performance problem is solved in terms of Riccati inequalities. A new design methodology is presented along with a numerical algorithm. An example is also included to demonstrate the proposed design method. © 1997 by John Wiley & Sons, Ltd.
Microtubule polymerase and processive plus-end tracking functions originate from distinct features within TOG domain arrays

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

XMAP215/Stu2/Alp14 accelerates tubulin polymerization while processively tracking microtubule (MT) plus ends via tumor overexpressed gene (TOG) domain arrays. It remains poorly understood how these functions arise from tubulin recruitment, mediated by the distinct TOG1 and TOG2 domains, or the assembly of these arrays into large square complexes. Here, we describe a relationship between MT plus-end tracking and polymerase functions revealing their distinct origin within TOG arrays. We study Alp14 mutants designed based on structural models, with defects in either tubulin recruitment or self-organization. Using in vivo live imaging in fission yeast and in vitro MT dynamics assays, we show that tubulins recruited by TOG1 and TOG2 serve concerted, yet distinct, roles in MT plus-end tracking and polymerase functions. TOG1 is critical for processive plus-end tracking, whereas TOG2 is critical for accelerating tubulin polymerization. Inactivating interfaces that stabilize square complexes lead to defects in both processive MT plus-end tracking and polymerase. Our studies suggest that a dynamic cycle between square and unfurled TOG array states gives rise to processive polymerase activity at MT plus ends.
The Essential Three (e3): A University Partnership to Meet the Professional Learning Needs of Rural Schools

Field: Social Sciences (Social Sciences)

The Essential Three (e3) is a professional learning series that focuses on supporting rural school districts and school leaders as they engage in the important work of prioritizing and determining areas of instructional focus. As educators transition to new rigorous state and national standards and face the challenges of learning newly adopted instructional frameworks/evaluation tools, the e3 training has offered educators much needed guidance and support within high-needs, rural districts in Florida. As the result of a partnership between the North-East Florida Educational Consortium (NEFEC) and the University of North Florida, teacher leaders and school administrators have now implemented a streamlined process to increase rigor, collaboration, and student engagement within the learning environment. This work details the comprehensive implementation process, as well as various promising practices for educators and rural school leaders to impact policies and instructional practice.
The upfront lipid-lowering combination therapy of statins and ezetimibe vs statin monotherapy in the reduction of cardiovascular outcomes. A meta-analysis.

Field: Medicine (Health Sciences)

Abstract Background Despite some data in last few years, there is still some inconsistency concerning the effectiveness and safety of the upfront intensive lipid lowering combination therapy (LLT; of moderate to high intensity statin therapy and ezetimibe) in comparison to high intensity statin therapy and recommended by some guidelines stepwise therapy approach. Purpose We sought to evaluate the efficacy of combination LLT compared with statin monotherapy for cardiovascular outcomes, LDL-C reduction, and associated adverse events. Methods A systematic literature search was conducted using PubMed, Embase, and ClinicalTrial.gov to identify relevant articles published from inception until 29th December 2023. The outcomes were assessed using pooled odds ratio (OR) for categorical data and mean difference (MD) for continuous data, with corresponding 95% confidence intervals (95% CI). Results A total of 11 studies (8 randomized controlled trials and 3 cohort studies) with 106,358 were involved in the analysis. The mean age of the patients in the combination LLT and statin monotherapy group was 65.3 and 65.7 years, respectively. Pooled analysis shows that combination LLT significantly reduces the LDL-C level from the baseline (MD, -12.13 mg/dl [0.31 mmol/l] (95%CI: -18.26 to -5.99 mg/dl), p&lt;0.001), all-cause mortality (ACM) (OR, 0.75, 95%CI: 0.62 to 0.92, p=0.01), cardiovascular mortality (CVM) (OR 0.75, 95%CI: 0.66 to 0.84, p&lt;0.001), major adverse cardiovascular events (MACE) (OR, 0.72 95%CI: 0.63 to 0.82, p&lt;0.001), non-significant reduction in the incidence of stroke (OR 0.82, 95%CI: 0.66 to 1.01, p=0.06) when compared with statin monotherapy alone. Similarly, the therapy discontinuation rate was comparable between combination LLT and statin monotherapy groups (OR, 0.87 (95%CI: 0.53 to 1.40), p=0.56). The risk of adverse events related to the gastrointestinal tract (OR, 1.12 (95%CI: 0.93 to 1.36), p=0.23) and musculoskeletal system (OR, 0.88 (95%CI: 0.52 to 1.50), p=0.65) was comparable between both the groups of patients. Conclusion Combination LLT was associated with an overall greater reduction in LDL-C, a lower risk of ACM, MACE, and CVM compared to statin monotherapy alone.Forest Plot of desired outcomes.Central illustration with key findings.
112 ALTER for home safety in brief: a process evaluation to examine the feasibility and acceptability of an adapted intervention program for a vulnerable population

Field: Health Professions (Health Sciences)

Unintentional injury is the leading cause of death in children 1–19 years of age; rates of injury in children of vulnerable parenting populations (e.g., those with lower socio-economic status, lower levels of education, younger maternal age) are even higher. Thus, intervention programs for parents need to be targeted and tailored for such a population. Currently, no evidence-based programming related to injury prevention is being used by Public Health agencies across Ontario, Canada. The previously validated Supervising for Home Safety intervention program was adapted to fit the needs of a vulnerable parenting population within the context of pre-existing community programming through these Public Health agencies. This process evaluation sought to explore parent acceptability, feasibility of facilitator implementation, and to determine practicality of a PRE/POST questionnaire as a future method to evaluate efficacy of this newly adapted program. Parents provided ratings of the program (e.g., helpfulness, utility, increase in knowledge, would they recommend the program), and facilitators provided ratings and written responses (e.g., preparation, clarity, improvements that could be made). Results suggest that overall both parents and facilitators rated the program positively. Major themes identified by facilitators suggest that the questionnaire should be significantly shortened and simplified to account for low literacy in this population specifically. Additionally, the limits of existing session length for this community programming is a barrier to implementing a thorough questionnaire evaluation. Moving forward, literacy level will be reduced overall, and alternatives to a questionnaire format will be explored. Conducting research within community setting constraints is discussed. This research has identified what remains to be addressed for the purposes of a large scale evaluation of a well-received program. This program has the potential to provide large scale publicly funded parenting programs with evidence based intervention to reduce the rates of unintentional injury in children among vulnerable parenting populations.
Biochemical characterization of G-Quadruplex recognition in the PITX1 mRNA by the specificity domain of the helicase RHAU

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Biologically, G-quadruplexes (G4s) are single-stranded structures that fold back on themselves, and are formed by DNA or RNA molecules in which one guanine base from each chain associate via cyclic Hoogsteen hydrogen bonding to form planar G-tetrad. Two or more of these tetrads can hydrophobically stack on top of one another to form G4 which are further stabilized by the presence of a mandatory monovalent cation centered between the planes. RNA Helicase Associated with AU-rich element (RHAU) is a member of the ATP-dependent DExH/D family of the RNA helicases that can bind and resolve G4 structures. To gain insight into the structural basis of G4 recognition by RHAU, a series of G4s were characterized using biophysical techniques including UV-Visible spectroscopy, electrophoretic mobility shift assays, dynamic light scattering, circular dichroism, small angle x-ray scattering and nuclear magnetic resonance spectroscopy. The mRNA for the Pituitary homeobox 1 (PITX1) protein was chosen as a model system to investigate G4 structures as it possesses three distinct G4 forming sequences in its 3ʹ- untranslated region (UTR) that interact with RHAU (Q1: PITX11371-1400, Q2: PITX11901-1930, and Q3: PITX12044-2079). First, Q2 was studied and demonstrated to adopt a parallel G4 orientation that can interact with the N-terminal domain of RHAU via its G-tetrad face. Interestingly, the DNA counterpart of Q2 adopted a different hybrid-type G4 structure with mixed parallel and antiparallel strands which was unable to interact with RHAU. Next, Q3 from PITX1 mRNA was studied as it contains long loops of 6 nucleotides to investigate the potential impact of interrupting loops on both the G4 structure and interaction with proteins/small molecules. Scrambling of only specific loop sequences impacted the global fold of Q3 while retaining a parallel G4 conformation. Disruption of the global fold through loop scrambling was shown to impact binding affinity of RHAU and a small molecule ligand that selectively interacts with the G-tetrad faces of parallel G4s. Taken together, the data in this thesis provides a biochemical and structural starting point for understanding the recognition of RNA G4s by binding partners.
CHAPTER 5. The Green Synthesis and Environmental Applications of Nanomaterials

Field: Environmental Science (Physical Sciences)

This chapter summarizes the use of green chemistry on the synthesis of nanomaterials as well as their environmental applications. Green routes for the synthesis of nanomaterials are discussed, including those using nontoxic solvents and natural template materials, such as ionic liquid, leaf and tea extract, natural cellulose, sucrose, starch, and plant leaves. Such methods could decrease the use of hazardous chemicals in chemical processes and reduce or eliminate undesirable products during the synthesis process. The employment of micro-organisms for the green synthesis of nanoparticles is also described. In addition, the use of nanomaterials as photocatalysts, Fenton's catalysts, and disinfectants is reported for environmental remediation including the degradation of organic contaminants and the inactivation of pathogenic micro-organisms in water. Finally, the importance of immobilization of nanomaterials onto substrates for their sustainable application to environmental remediation, in particular, contaminated water and wastewater treatment is presented.
MATRIX APPLICATIONS TO VIBRATION PROBLEMS AND ELECTRICAL NETWORKS,

Field: Engineering (Physical Sciences)

This report reviews the present and future education of engineering students and reports that the availability of modern computing means has accelerated the use of matrix methods. It discusses matrix applications and shows how two totally dissimilar problems led to the same computational problem. Similar examples can be drawn from other fields of engineering. The ease with which the problems are handled is due to the advent of digital computing technology, and that in the future we shall see more use of these methods, even at the freshman level. Because of the ease with which tedious calculations can be accomplished, problems of large extent become feasible in engineering instruction. (Author)
Camouflage Color Changing Robot

Field: Engineering (Physical Sciences)

Science is a field developing with a rapid pace, creating the technology that can make human life easier.One such invention of this field is ROBOT.The word robot means a machine that can be programmed to carry out complex series of actions.Military operations are considered to be the most dangerous work environment.The camouflage robot is the solution for reducing loss of human life by replacing it in such operations.The proposed system consists one camera for surveillance & one camera for color sensing purpose.The robot senses the color of the surrounding through this camera & changes its color to blend in with it.This makes difficult to be spotted by the unaided enemy eyes.The robot can be remotely controlled via a computer & wireless Trans Receiver is used to establish the connection between them.