jimnoneill/paper-to-field-training huggingface dataset:
Involvement of multiple DNA elements in iron‐inducible transcription of the ap65‐1 gene in the protozoan parasite Trichomonas vaginalis

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Summary A reputed iron‐responsive region, which contains multiple nuclear protein‐binding DNA sequences, was shown previously to regulate iron‐inducible transcription of the ap65‐1 gene in the protozoan pathogen, Trichomonas vaginalis . These DNA sequences include two overlapping MYB recognition elements (MRE‐1/MRE‐2r) and three abutted T‐tract elements. Additional nuclear protein‐binding DNA sequences flanking the 5′ (AGTGAAGTGA) and 3′ (MRE‐2f) of the iron‐responsive region were identified in the present study. A stable promoter assay and primer extension revealed that transcriptional activity of the ap65‐1 promoter is iron inducible as well as growth related, being lowest in the early logarithmic phase and highest in the mid‐logarithmic phase. Subsequent mutational analysis of individual DNA elements of the ap65‐1 promoter suggests that closely spaced T‐tract elements together with an intervening GAAGGAAG sequence within the iron‐responsive region are most critical for regulation of overall transcriptional activity, whereas an additional AGTGAAGTGA and MRE‐2f together with an upstream T‐rich region are required for optimal iron‐inducible activity, and the MRE‐1/MRE‐2r overlap is only involved in growth‐related activity. These observations suggest that expression of the ap65‐1 gene is dynamically regulated under various growth conditions via interactions among multiple DNA regulatory elements.
Study on the Application Effect of Strong Rooting Powder on Pepper Plug Seedlings

Field: Agricultural and Biological Sciences (Life Sciences)

Using strong rooting powder as test reagents,according the proportion that per kg soilless media with 50% relative humidity were added 5,10,15,20 mg strong rooting powder to deal with the substrates,and taking no added strong rooting powder as contrast to train pepper seedlings.The results showed that soilless media added certain amount of strong rooting powder had promotion to pepper seedlings growth,in which,per kg soilless media added 10 mg strong rooting powder had the best promotion to the growth potential and root activity of pepper seedlings,although the other process had differences with the contrast,but not significant.
Experimental Study on Hot Hand Effect and Gambler's Fallacy of Chinese Investors

Field: Psychology (Social Sciences)

With psychological experiments, we found that whenever stock price goes up or goes down continuously, gambler's fallacy will dominate hot-hand effect in investor's information process to serial changing stock price. When stock price goes up continuously, the longer the price up, the larger is the probability that investor think the price will drop down in next period, and the tendency to sell it get more evident. On the other hand, when stock price drops down continuously, the longer the price down, the larger is the probability that investor think the price will go up in next period, and the tendency to buy it became larger. These results suggest that at least psychological factor exist in the individual investor that hope the market will reverse to bullish in today's bearish Chinese stock market. Disposition effect is also found in the experiments, which is more evident in female investor than in male investor and more evident in the investor with low level investment knowledge and less experience than those with higher level. Experiments also indicate that Chinese investors like short-term investing which is much shorter than one year.
Concomitant aortic valve and ascending aorta replacement with moderate hypothermic circulatory arrest to treat an aortic bicuspid valve with post-stenotic dilatation

Field: Medicine (Health Sciences)

We recommend concomitant surgery for aortic valve replacement (AVR) and ascending aortic replacement using moderate hypothermic circulatory arrest (CA) for post- stenotic dilatation complicated by an aortic bicuspid valve. Cardiopulmonary bypass (CPB) was established from the right atrium to the dilated ascending aorta. As soon as the rectal temperature decreased to 28 C, CA was commenced and the open distal anastomosis of a polyester prosthesis, without any cerebral perfusion, was completed. AVR was then carried out during rapid re-warming with CPB using a side arm of the prosthesis. This procedure exhibited safe and satisfactory results. There are many benefits of carrying out the procedure in this way; it avoids the requirement for cannulation to a calcified aortic arch, provides a good oper- ative field, for an easier distal anastomosis and suturing at the valve site, and reduces the risk of further dilatation or dis- section of the residual ascending aorta in the later phase.
Assessment of metabolic activity and energy supply of peripheral blood lymphocytes

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

BACKGROUND: T-cells have the capability to change their metabolism in response to activation signals. Resting T-cells primarily use the oxidation of higher fatty acids and oxidative phosphorylation in mitochondria for their energy needs, whereas activated T-cells switch to aerobic glycolysis and glutaminolysis, using glucose, and glutamine as substrates, respectively. AIM: To determine the metabolic activity and energy supply of peripheral blood lymphocytes in predominantly healthy northerners by measuring the intracellular content of HIF-1 (hypoxia-induced factor 1), SIRT3 (sirtuin 3), and ATP (adenosine triphosphate). MATERIALS AND METHODS: 39 volunteers, residents of the Arkhangelsk region (23 women and 16 men, 2362 years old), were selected, and examined for this experiment. We established the total number of peripheral blood lymphocytes with CD-typing of lymphocytes (CD3+, CD4+, CD8+, CD71+) by indirect immunoperoxidase reactions, the content of HIF-1 and SIRT3 in the lymphocyte lysate employing enzyme immunoassay, the concentration of ATP by luminescent analysis through luciferin-luciferase reaction. Statistical analysis was conducted in "Statistica 10.0", cluster analysis was applied using the k-means method. Mean values (M) and standard deviations (SD) were calculated, the normal distribution was tested by the KolmogorovSmirnov and Lilliefors criterion. Student's t-test was calculated, and the differences were considered statistically significant at p 0.05. RESULTS: The study revealed that the metabolic activity of lymphocytes associated with HIF-1 regulation differs significantly in the examined volunteers,, while in the group with a lower total number of lymphocytes and their subpopulations (CD3+, CD4+, CD8+, CD71+) there is a predominant glycolytic orientation of metabolism with proliferated cells energy supply. CONCLUSION: Metabolic activity which can be determined by the HIF-1/SIRT3 ratio, and the energy supply of lymphocytes have a substantial impact on their differentiation, proliferation, and functioning.
Supplementary Data from CBP Modulates Sensitivity to Dasatinib in Pre-BCR<sup>+</sup> Acute Lymphoblastic Leukemia

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

<p>Unbiased shRNA library screening and global transcriptome analyses reveal mechanisms that modulate dasatinib sensitivity and suggest therapeutic strategies to improve outcome of patients with acute lymphoblastic leukemia.</p>
CITIZENSHIP EDUCATION WITH MORAL-BASED POSSIBILITIES: INQUIRIES IN AN AMERICAN FIFTH GRADE CLASSROOM

Field: Arts and Humanities (Social Sciences)

Moral growth of students can become neglected due to schooling’s focus on intellectual growth. In this paper, I explore the value of “imaginative engagement” and “empathetic understanding,” theoretically conceptualized, as a way to address the issue of a more flexible curriculum in fiction literature for conscientious citizenship education. After a brief review of moral education factors, I indicate a conception of democratic citizenship. Next, I demonstrate specific examples and situations for moral-based experiences related to fifth grade curriculum—namely, literature in the classroom. I conclude with a detailed exploration of the value of moral-based possibilities within the school context for development of democratic citizens.
Mediators of a long-term movement abnormality in a Drosophila melanogaster model of classic galactosemia

Field: Biochemistry, Genetics and Molecular Biology (Life Sciences)

Summary Despite neonatal diagnosis and life-long dietary restriction of galactose, many patients with classic galactosemia grow to experience significant long-term complications. Among the more common are speech, cognitive, behavioral, ovarian, and neurological/movement difficulties. Despite decades of research, the pathophysiology of these long-term complications remains obscure, hindering prognosis and attempts at improved intervention. As a first step to overcome this roadblock we have begun to explore long-term outcomes in our previously reported GALT-null Drosophila melanogaster model of classic galactosemia. Here we describe the first of these studies. Using a countercurrent device, a simple climbing assay, and a startle response test to characterize and quantify an apparent movement abnormality, we explored the impact of cryptic GALT expression on phenotype, tested the role of sub-lethal galactose exposure and galactose-1-phosphate (gal-1P) accumulation, tested the impact of age, and searched for potential anatomical defects in brain and muscle. We found that about 2.5% residual GALT activity was sufficient to reduce outcome severity. Surprisingly, sub-lethal galactose exposure and gal-1P accumulation during development showed no effect on adult phenotype. Finally, despite the apparent neurological or neuromuscular nature of the complication we found no clear morphological differences between mutants and controls in brain or muscle, suggesting that the defect is subtle and/or may be physiologic rather than structural. Combined, our results confirm that, like human patients, GALT-null Drosophila experience significant long-term complications that occur independent of galactose exposure, and serve as a proof of principle demonstrating utility of the GALT-null Drosophila model as a tool to explore genetic and environmental modifiers of long-term outcome in GALT deficiency.
TISSUE CULTURE AND AGAMIC PROPAGATION OF WINTER-FROST TOLERANT 'LONGICAULIS' ARUNDO DONAX L.

Field: Agricultural and Biological Sciences (Life Sciences)

The primary objectives of this study were to identify the ecotypes of the 'longicaulis' giant reed (Arundo donax L.) that are winter-frost and cold tolerant in temperate climates on marginal lands and to perform biotechnological research on these ecotypes.We searched for large 'longicaulis' giant reed ecotypes, which wintered successfully in spite of the 2012/2013 winter frosts and sprouted from stem nodes in the spring of 2013 in three locations of the Balaton basin.Starting in vitro culture from the buds of the secondary shoot of the full-grown plant on Murashige and Skoog (1962) medium supplemented with 0.3 mg L -1 6-benzyl-aminopurine (BAP) and 0.05 mg L -1 1-naphthalene-acetic acid (NAA) was the most satisfactory during October.Our experiments indicated that in vitro propagation by stem cuttings can be successfully performed from in vitro shoots (node number increased with 0.2% NaCl treatment) on Murashige and Skoog (1962) medium containing kinetin (3 mg L -1 ) and indole-3-acetic acid (3 mg L -1 ).By combining our micro-and macropropagation methods based on propagation by stem cuttings, 400 to 450 plants can be produced from one in vitro shoot during 11 to 12 months of growth.
Synthesis of Novel Dinuclear N‑Substituted\n4‑(Dimethylamino)benzaldehyde Thiosemicarbazonates of Rhenium(I):\nFormation of Four- and/or Five-Membered Chelate Rings, Conformational\nAnalysis, and Reactivity

Field: Chemistry (Physical Sciences)

The\nreaction of <i>fac</i>-[ReX­(CH<sub>3</sub>CN)<sub>2</sub>(CO)<sub>3</sub>] (X = Cl, Br) with <i>N</i>-phenyl-[4-(dimethylamino)­benzaldehyde]\nthiosemicarbazone (<b>HL</b><sup><b>A</b></sup>) or <i>N</i>-4-methoxybenzyl-[4-(dimethylamino)­benzaldehyde] thiosemicarbazone\n(<b>HL</b><sup><b>B</b></sup>) under controlled synthetic\nconditions gave 4 mononuclear [ReX­(HL)­(CO)<sub>3</sub>] (X = Cl, Br)\nand 16 dinuclear [Re<sub>2</sub>L<sub>2</sub>(CO)<sub>6</sub>] compounds.\nThese complexes were obtained as single crystals, and their structures\nwere established by X-ray diffraction. The structural study of these\ndimers showed the formation of several solvates, the presence of linkage\nisomerism, and the stabilization of four- and/or five-membered chelate\nrings. The different ligand coordination modes (a new μ-κ<sup>2</sup>-S,N2:κ-N3 coordination mode for a thiosemicarbazone\nligand was observed), the conformation of the thiosemicarbazone chain\nin each case, the formal symmetry of the dimers, and the role of the\nsynthetic procedure in the stability of the different chelate rings\nwere analyzed and are discussed. Theoretical calculations in the gas\nphase were performed for the dimers with the <b>HL</b><sup><b>A</b></sup> ligand in order to identify the thermodynamically\nmost stable species. The behavior and structural stability of dimers\nin dimethyl sulfoxide and acetone solutions was investigated by <sup>1</sup>H NMR spectroscopy. The strength of the Re<sup>I</sup>–L\nbond in solution was evidenced by the formation of [Re<sub>2</sub>(L<sup>NO<sub>2</sub></sup>)<sub>2</sub>(CO)<sub>6</sub>] and [Re­(L<sup>A</sup>)­(py)­(CO)<sub>3</sub>] upon reaction of the corresponding\ndimer with concentrated nitric acid and pyridine, respectively.
Research progress of Th22 cells and their roles in autoimmune diseases

Field: Immunology and Microbiology (Life Sciences)

Th22 cells,distinctly separated from Th1,Th2 and Th17 cells,are a newly identified subset of CD4+ T helper cells.With the CCR6+ CCR4+ CCR10+ phenotype,Th22 cells are characterized by the secretion of IL-22,but not IL-17 or IFN-γ.Th22 cells represent a T cell subset with stable and unique expressional and functional profile.As part of the immune system,Th22 or IL-22 plays a role in the innate immunity of tissue cells,protecting tissues from damage and enhancing tissue regeneration.Recent evidence has revealed that Th22 subset and its major effector IL-22 play a critical role in the pathophysiology of autoimmune diseases.Further research to elucidate the biological characteristics of Th22 subset and its roles in autoimmune diseases would be of great benefit for understanding the pathogenesis of autoimmune diseases,and for the development of therapeutic strategy.

Key words:
Th22 cells ; IL-22 ; Autoimmune diseases ;
Revisiting the Right to Know: The Transnational Adoptee and the Moral Economy of ‘Return’

Field: Social Sciences (Social Sciences)

In this article I examine regulatory regimes of mobility through the case of transnational adoption. In particular, I focus on ‘return’ journeys by adoptees and their entanglement the ‘right to know’. Backed up by international law, the right to know in the adoption context includes the right to know one’s biological parents, one’s birth culture, and the right to information (for instance, medical information). At large, the ‘right to know’ signals a valuation of openness. But what are the problems that come with knowledge and information? What does the imperative of openness do to kinship relations? Looking at a number of instances where institutions discuss the necessities and implications of return journeys, I demonstrate in this article that ‘the right to know’ is inextricably linked to moral economies of kinship. Returns make visible the transaction of adoption and the exchangeability of the adoptee body. I discuss how adoptees are implicated in this moral economy but also how they, as subjects, negotiate, destabilise, or refuse openness as imperative. I argue that in the end, while the ‘right to know’ allows the discovery of relations, it also fails to acknowledge the invention of relations.
Abstract 339: Combining Urgent Coronary Angiography With Therapeutic Hypothermia Does Not Slow Cooling Times for Comatose Survivors of Cardiac Arrest

Field: Medicine (Health Sciences)

Introduction: Selected comatose survivors of out-of-hospital-cardiac arrest (OHCA) meet criteria for both emergent percutaneous coronary intervention (PCI) and induction of mild therapeutic hypothermia (MTH). It is unclear which should be performed first. We hypothesized that urgent catheterization prior to MTH may significantly prolong time to cooling. Methods: We conducted a retrospective cohort study, reviewing electronic medical records of all patients treated with MTH in the University of Michigan Emergency Department (ED) for OHCA. We identified whether the endovascular cooling device was inserted in the ED or catheterization lab and assessed time from ED arrival to cooling device insertion, to cooling initiation, and to reaching ≤33.5°C. Neurological outcomes were determined by Cerebral Performance Category (CPC) via chart review. Times were compared via Student’s t-tests. Results: Between 7/1/2006 and 9/14/2012, 123 consecutive patients were treated with MTH. Twenty-six (22%) had their device placed in the catheterization lab (14/26 received PCI) while 92 (77%) had theirs placed in the ED. There was no significant difference between catheterization lab v. ED device placement in time from ED arrival to device insertion, arrival to cooling initiation, and arrival to reaching target temperature (see table). Forty-six percent vs 39% of those whose devices were inserted in the catheterization lab vs ED survived past hospital discharge (OR 1.8; 95% CI 0.7-4.4), and 35% vs 39% had favorable 6-month neurological outcomes (OR 1.3; 95% CI 0.5-3.3). Conclusions: In our system, initiating cooling in the catheterization lab as opposed to the ED does not significantly delay cooling times. The resources and efficiencies involved in activation of the cath lab team may offset the time taken to perform angiography and potential subsequent PCI. These data support the practice of immediate cardiac catheterization in appropriate comatose survivors of cardiac arrest.
On the constricted neon positive column

Field: Physics and Astronomy (Physical Sciences)

The axial electric field strength, electron temperature, and radially averaged electron density in a medium pressure and current, constricted neon plasma column of a direct current glow discharge have been measured by electric methods. The ion loss and production rate coefficients and the loss terms have been calculated and the ratio between recombination loss and diffusion loss evaluated. The steady-state equilibrium between ion production and ion loss shows that, though electron-impact ionization of metastable species is an important ion-production channel, electron-impact ionization via the resonance and higher excited neon levels and associative ionization are also important ion-production channels.
‘Montage, My Fine Care’: Realism, Surrealism and Postmodernism after Bazin

Field: Arts and Humanities (Social Sciences)

The title of this chapter takes its cue from one of Jean-Luc Godard’s well-known articles, published in Cahiers du cinéma in 1956, which engaged with Bazin’s conception of cinematic realism in an attempt to effect a generational break with the past by proposing a revised understanding of montage, not simply as an integral part of mise-en-scène, but as a form of deliberate authorial statement and, in that sense, as a practical extension of the New Wave ideology or politique des auteurs. In highlighting Bazin’s formative influence on the New Wave directors (in particular, his relationship to Truffaut and Godard), this chapter also focuses on the revived interest in early avant-garde experimentation (as evidenced, for instance, by Godard’s use of silent era shot transitions, image/sound disjunction and quotations from Surrealist poets in A bout de souffle), as well as the emergence of postmodern strategies and notions of rhythm, movement and time in French cinema (again present in the early work of Jean-Luc Godard), which prefigured Deleuze’s re-appraisal of montage as part of his theory of the movement-image and the time-image.
TMSI-Promoted Vinylogous Michael Addition of Siloxyfuran to 2-Substituted Chromones: A General Approach for the Total Synthesis of Chromanone Lactone Natural Products

Field: Chemistry (Physical Sciences)

A concise and facile synthetic protocol for the construction of the 2-γ-lactone chromanone skeleton has been achieved through a TMSI-promoted diastereoselective vinylogous Michael addition of siloxyfuran to 2-substituted chromones. The applicability of this method is demonstrated through the rapid access to the total syntheses of (±)-microdiplodiasone, (±)-lachnone C, and (±)-gonytolides C and G.
The Effects of Media and their Logic on Legitimacy Sources within Local Governance Networks: A Three-Case Comparative Study

Field: Social Sciences (Social Sciences)

__Abstract__ \n \nAlthough theoretical and empirical work on the democratic legitimacy of \ngovernance networks is growing, little attention has been paid to the impact of mediatisation \non democracies. Media have their own logic of news-making led by the media’s rules, \naims, production routines and constraints, which affect political decision-making processes. \nIn this article, we specifically study how media and their logic affect three \ndemocratic legitimacy sources of political decision-making within governance networks: \nvoice, due deliberation and accountability. We conducted a comparative case study of \nthree local governance networks using a mixed method design, combining extensive \nqualitative case studies, interviews and a quantitative content analysis of media reports. \nIn all three cases, media logic increased voice possibilities for citizen groups. \nFurthermore, it broadened the deliberation process, although this did not improve the \nquality of this process per se, because the media focus on drama and negativity. Finally, \nmedia logic often pushed political authorities into a reactive communication style as they \nhad to fight against negative images in the media. Proactive communication about \nprojects, such as public relation (PR) strategies and branding, is difficult in such a \nmedia landscape.
Temperature modeling of air-breathing PEMFC stack and improved GPC control

Field: Engineering (Physical Sciences)

To make fuel cell have good performance,the stack temperature of proton exchange membrane fuel cell(PEMFC) should be controlled within a specified range.Based on generalized predictive control(GPC),a predictive control scheme of PEMFC temperature was proposed in this paper.Firstly,under the law of conservation of energy inside the cell,a temperature model of air-breathing PEMFC stack was developed.Then,based on this model,the GPC controller was adopted in the design of PEMFC temperature control system.And a modified GPC algorithm was developed to reduce the calculation amount and overshoot in the control process.Otherwise,the optimal operation temperature obtained from lots of experiments were used as setting temperature.The simulation result indicates that the GPC control system of PEMFC temperature improves the control accuracy and the response speed.