199 IMPLANTATION OF BONE MARROW-DERIVED CELLS RECOVERS TISSUE STRUCTURES AND FUNCTIONS OF RADIATION-INJURED URINARY BLADDERS IN RATS

Field: Medicine (Health Sciences)

You have accessJournal of UrologyStem Cell Research1 Apr 2012199 IMPLANTATION OF BONE MARROW-DERIVED CELLS RECOVERS TISSUE STRUCTURES AND FUNCTIONS OF RADIATION-INJURED URINARY BLADDERS IN RATS Tetsuya Imamura, Osamu Ishizuka, Yoshiki Kurizaki, Hitoshi Yokoyama, Wataru Noguchi, Takahiro Yamagishi, Zhang Lei, Haruaki Kato, and Osamu Nishizawa Tetsuya ImamuraTetsuya Imamura Matsumoto, Japan More articles by this author , Osamu IshizukaOsamu Ishizuka Matsumoto, Japan More articles by this author , Yoshiki KurizakiYoshiki Kurizaki Matsumoto, Japan More articles by this author , Hitoshi YokoyamaHitoshi Yokoyama Matsumoto, Japan More articles by this author , Wataru NoguchiWataru Noguchi Matsumoto, Japan More articles by this author , Takahiro YamagishiTakahiro Yamagishi Matsumoto, Japan More articles by this author , Zhang LeiZhang Lei Matsumoto, Japan More articles by this author , Haruaki KatoHaruaki Kato Matsumoto, Japan More articles by this author , and Osamu NishizawaOsamu Nishizawa Matsumoto, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.252AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES This study investigated to determine if the implantation of bone marrow-derived cells could recover the radiation-injured urinary bladders in rats. METHODS Thirty female 10-weeks Sprague-Dawley (SD) rats were anesthetized, and then retained and protected with an iron board. The region including bladder within 1cm-diameter cycle bordering on pubis was radiated with 2Gy (the absorbed energy of 1 J/kg). The radiation was performed every week, and repeated 5 times. After the radiations, the rats were maintained for 2 weeks. The radiated rats were separated into two groups: cell implantation group (n=10), and cell-free injection control group (n=10). Other rats were used as pre-implantation group (n=10). Bone marrow cells were harvested from both femurs of six male 17-weeks Tg-SD (GFP) rats. The cells were cultured for 7 days, and then the adherent proliferating cells were used as bone marrow-derived cell. The 2.0x106 cells were implanted into the radiated bladder wall. As control, the cell-free solution was similarly injected. At 4 weeks after implantation, cystometric investigations, common stains and immunohistochemistry were performed. RESULTS Just prior to the cell-implantation, tissue structures of the radiation-injured bladders were disorganized. At 4 weeks after, the cell-implanted bladders had numerous smooth muscle and nerve cells compared to the control ones. These cells organized tissue structures (Fig. 1A). In the reconstructed structures, some GFP-positive cells were positive for SMA- (Fig. 1B) or S100-antibody (Fig. 1C). The voiding interval and residual volume of radiation-injured rats tended to lengthen and increase compared to the non-radiated sham ones. At 4 weeks after, the voiding interval (5.46±0.33 min) and residual volume (0.04±0.01 ml) of cell-implanted rats were significantly decreased compared to the control ones (7.39±0.54 min, P<0.05 and 0.15±0.05 ml, P<0.01, respectively), and these values were as same as the sham ones. CONCLUSIONS The radiation-injured bladders showed disorganization of the tissue structures, and the lengthened voiding interval and increased residual volume. The implantation of bone marrow-derived cells reconstructed tissue structures, and improved the voiding interval and residual volume. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e83-e84 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tetsuya Imamura Matsumoto, Japan More articles by this author Osamu Ishizuka Matsumoto, Japan More articles by this author Yoshiki Kurizaki Matsumoto, Japan More articles by this author Hitoshi Yokoyama Matsumoto, Japan More articles by this author Wataru Noguchi Matsumoto, Japan More articles by this author Takahiro Yamagishi Matsumoto, Japan More articles by this author Zhang Lei Matsumoto, Japan More articles by this author Haruaki Kato Matsumoto, Japan More articles by this author Osamu Nishizawa Matsumoto, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...