Chromosome and genomic engineering in plants : methods and protocols This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a plant chromosome vector system which allows the introduction of desired genes or DNA into target sites on the chromosome vector particularly by sequence specific recombination. Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences Method for Biolistic Site Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking Generation and Analysis of Transposon Ac Ds Induced Chromosomal Rearrangements in Rice Plants One Step Generation of Chromosomal Rearrangements in Rice Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana Gametocidal Systems for Dissecting Wheat Chromosomes CRISPR Cas Mediated Site Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases Targeted Mutagenesis in Rice Using and TALENs and the CRISPR Cas9 System Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination Development of Genome Engineering Tools from Plant Specific PPR Proteins Using Animal Cultured Cells Chromosomal Allocation of DNA Sequences in Wheat Using Flow Sorted Chromosomes Image Analysis of DNA Fiber and Nucleus in Plants Detection of Transgenes on DNA Fibers Three Dimensional Live Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes Mapping of T DNA and Ac Ds by TAIL PCR to Analyze Chromosomal Rearrangements