RNA methodologies : laboratory guide for isolation and characterization CHAPTER 1: RNA and the Cellular Biochemistry Revisited CHAPTER 2: RNA Isolation Strategies CHAPTER 3: The Truth about Tissues CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) CHAPTER 5: Isolation of Polyadenylated RNA CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) CHAPTER 7: Resilient Ribonucleases CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) CHAPTER 9: Electrophoresis of RNA (EXPANDED) CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) CHAPTER 11: Northern Analysis CHAPTER 12: Nucleic Acid Probe Technology CHAPTER 13: Practical Nucleic Acid Hybridization CHAPTER 14: Principles of Detection (EXPANDED) CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) CHAPTER 17: cDNA Synthesis (EXPANDED) CHAPTER 18: RT PCR (NEW) CHAPTER 19: Quantitative PCR Techniques CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) CHAPTER 21: High Throughput Analysis of Gene Expression CHAPTER 22: Non Array Methods for Global Analysis of Gene Expression (NEW) CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) CHAPTER 24: Genomes Transcriptomes Proteomes and Bioinformatics CHAPTER 25: An RNA Paradigm This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue expansion of PCR optimization analysis and RNA interference sections the introduction of a new chapter dealing with the molecular biology of plants and an expanded glossary