Targeted genome editing using site specific nucleases : ZFNs TALENs and the CRISPR Cas9 system This book serves as an introduction to targeted genome editing beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail in iPS cells and diverse organisms such as mice rats marine invertebrates fish frogs and plants. Tools that are mentioned include zinc finger nucleases (ZFNs) transcription activator like effector nucleases (TALENs) and CRISPR Cas9 all of which have received much attention in recent years as breakthrough technologies.