Phosphoproteome analysis of the near haploid cell line HAP1 reveals phosphorylation events originating from PDZ binding kinase T LAK cell originated protein kinase (PBK TOPK) PBK is a serine threonine kinase. During mitosis PBK phosphorylates the inter zinc finger linker sequence (TGEKP) of hundreds of C2H2 zinc finger proteins and liberates these transcription factors from the condensing chromatin. This study aimed to investigate PBK dependent phosphorylation events by analyzing the phosphoproteome of both wild and PBK knockout HAP1 cells. The analysis identified candidates belong to DNA and RNA binding proteins (ADNP and FUBP1) or dynamic regulators of cell organelles (MARCKS and PGRMC1).<eng>