NMR in glycoscience and glycotechnology 3.3.6 Strategies to Obtain Good Intermolecular NOEs for High Affinity Complexes3.3.7 Low Affinity A Problem for Protein Carbohydrate Structure Determination; 3.3.8 Influence of the Field Strength and Other Parameters on Intermolecular NOEs; 3.3.9 Other Complications and Solutions; 3.3.10 Promising Technological Developments Applicable to Protein Carbohydrate Complexes; 3.4 Judging the Quality of Protein Carbohydrate Complex Structures Determined by NMR; 3.5 Conclusions; Acknowledgements; References; Chapter 4 Paramagnetic RDC and RCSA Constraints in the Structural Analysis of Glycans This volume focuses on solution and solid state NMR of carbohydrates glycoproteins glyco technologies biomass and related topics. Timely and useful it will appeal to researchers in the general field of structural biology biochemistry and biophysics molecular and cellular biology and material science Cover; NMR in Glycoscience and Glycotechnology; Preface; Contents; Chapter 1 Intramolecular Hydrogen Bonding in Glycans in Aqueous Solution; 1.1 Introduction; 1.2 NH Hydrogen Bonding; 1.3 OH Detection and Hydrogen Bonding; 1.3.1 Measuring OH Exchange Rate Constants for Inferring Hydrogen Bonding; 1.4 Hydrogen Bonding Involving C H Bonds as Donors; 1.5 Hydrogen Bonding Between OH and Carboxylate Groups; 1.6 Future Directions; References; Chapter 2 NMR Spin Couplings in Saccharides: Relationships Between Structure Conformation and the Magnitudes of JHH JCH and JCC Values Chapter 3 Insights into Carbohydrate Recognition by 3D Structure Determination of Protein Carbohydrate Complexes Using NMR3.1 Introduction; 3.2 Overview of Protein Carbohydrate Structures Determined by NMR; 3.3 Three Dimensional Structure Determination by NMR Spectroscopy; 3.3.1 The Importance of Intermolecular NOEs; 3.3.2 NMR Pulse Sequences to Detect Intermolecular NOEs; 3.3.3 Isotope Labelling of Oligosaccharides; 3.3.4 Line Broadening at the Recognition Interface; 3.3.5 Binding Equilibria and Their Consequences on Detecting Intermolecular NOEs 2.7 Core Relationships Between J Coupling and Saccharide Structure2.7.1 High Abundance of Oxygen and Nitrogen Lone Pair Orbitals in Saccharides; 2.7.2 Two Bond (Geminal) 13C 1H and 13C 13C Spin Couplings; 2.7.3 Three Bond (Vicinal) 13C 1H and 13C 13C Spin Couplings; 2.7.4 Dual Pathway 13C 13C Spin Couplings in Aldopyranosyl and Aldofuranosyl Rings; 2.7.5 Four Bond 1H 1H and 13C 1H Spin Couplings in Aldopyranosyl Rings; 2.7.6 Spin Couplings Across O Glycosidic Linkages in Oligosaccharides; 2.8 Measurement of NMR Spin Couplings in Solids; 2.9 Concluding Remarks; Acknowledgements; References 2.1 Introduction and Background2.2 Empirical Predictions of 2JCH and 2JCC Values in Saccharides; 2.3 Experimental Determinations of Spin Coupling Signs in Saccharides; 2.4 Second Order Behavior in 1H and 13C NMR Spectra of Saccharides; 2.5 Structural Elements of Saccharides and Factors that Influence Them; 2.5.1 Ring Conformation; 2.5.2 Exocyclic Hydroxymethyl Conformation; 2.5.3 Exocyclic C O Bond Conformation; 2.5.4 Exocyclic N Acetyl Group Conformation; 2.5.5 O Glycosidic Linkage Conformation; 2.6 Assets and Limitations of J Couplings to Determine Saccharide Structure