Optical tweezers : methods and protocols Introduction to optical tweezers Exact theory of optical tweezers and its application to absolute calibration Beyond the hookean spring model: Direct measurement of optical forces through light momentum changes A surface coupled optical trap with 1 bp precision via active stabilization Implementation and tuning of an optical tweezers force clamp feedback system Custom made microspheres for optical tweezers Optical torque wrench design and calibration High resolution fleezers : Dual trap optical tweezers combined with single molecule fluorescence detection Versatile quadruple trap optical tweezers for dual DNA experiments Probing DNA DNA interactions with a combination of quadruple trap optical tweezers and microfluidics Probing single helicase dynamics on long nucleic acids through fluorescence force measurement Mechanically watching the clpXP proteolytic machinery Deciphering the molecular mechanism of the bacteriophage phi29 DNA packaging motor Single molecule protein folding experiments using high precision optical tweezers Observing single RNA polymerase molecules down to base pair resolution Optical tweezers based measurements of forces and dynamics at microtubule ends Simultaneous manipulation and super resolution fluorescence imaging of individual kinetochores coupled to microtubule tips Measurement of force dependent release rates of cytoskeletal motors Measuring the kinetic and mechanical properties of non processive myosins using optical tweezers Quantifying force and viscoelasticity inside living cells using an active passive calibrated optical trap Measuring molecular forces using calibrated optical tweezers in living cells