Microscopy techniques 1 Miyawaki T. Nagai H. Mizuno: Engineering Fluorescent Proteins. 2 C. K. Sun: Higher Harmonic Generation Microscopy. 3 R. Gräf J. Rietdorf T. Zimmermann: Live Cell Spinning Disk Microscopy. 4 T. Wazawa M. Ueda: Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience. 5 T. Kohl P. Schwille: Fluorescence Correlation Spectroscopy with Autofluorescent Proteins. 6 E.B. van Munster T.W.J. Gadella: Fluorescence Lifetime Imaging Microscopy. 7 A.B. Houtsmuller: Fluorescence Recovery After Photobleaching: Application to Nuclear Proteins. 8 J. B. Sibarita: Deconvolution Microscopy. 9 T. Zimmermann: Spectral Imaging and Linear Unmixing in Light Microscopy