Indoloquinoline oligonucleotide conjugates in the antigene strategy : binding and NMR structural studies Triple helix forming oligonucleotides (TFOs) are one of the most specific DNA duplex binding agents and offer new perspectives towards oligonucleotide mediated gene regulation and manipulation. However the poor thermodynamic stability of DNA triplexes under physiological conditions limits a successful application in the antigene strategy. Thus the conjugation of TFOs with small triplex specific binding ligands is a promising approach to stabilize the formed complexes and to enhance their overall binding affinity. The present study focused on the synthesis of novel TFO conjugates with triplex binding indolo[3 2 b]quinoline derivatives (PIQ) and on their ability to form and stabilize intermolecular triplexes through their recognition of a duplex target. During the course of the work the thermodynamics of conjugate binding and structural aspects of drug DNA interactions have been characterized by a variety of spectroscopic and calorimetric techniques. DNS Spektroskopie Thermodynamik DNA Wirkstoff Interaktion DNA Triplex Oligonukleotid Konjugate Indolochinolin DNA drug interaction DNA triplex oligonucleotide conjugate indoloquinoline