Increasing culturability of plant microbiome towards core microbiome manipulation and engineering This study aimed to optimize the use of plant based culture media for exploration of the plant associated microbiota and extend the culturability borders to isolate novel bacterial taxa and meticulously characterize the culture dependent and culture independent microbiomes in view of the developed plant based culture media. Throughout the study we could prove that plant based culture media are able to support the recovery of previously uncultured plant associated bacteria and also support the enrichment of bacterial lineages that belong to the candidate phyla radiation (CPR).<eng>