The condensed protocols from molecular cloning: a laboratory manual Plasmids and their usefulness in molecular cloning Bacteriophage and its vectors Working with bacteriophage M13 Working with high capacity vectors Gas electrophoresisof DNA and pulsed field agarose Preparation and analysis of eukaryotic genomic DNA Extraction purification and analysis of mRNA from eukaryotic cells In vitro amplification of DNA by the polymerase chain reaction Preparation of radiolabeled DNA and RNA probes Working with synthetic oligonucleotide probes Preparation of cDNA libraries and gene identification DNA sequencing Mutagenesis Screening expression libraries Expression of cloned genes in escherichia coli Introducing cloned genes into cultured mammalian cells Analysis of gene expression in cultured mammalian cells Protein interaction technologies.