Devising computational tools to quantify the actin cytoskeleton and pavement cell shape using network based approaches Recent advances in microscopy have led to an improved visualization of different cell processes. Yet this also leads to a higher demand of tools which can process images in an automated and quantitative fashion. Here we present two applications that were developed to quantify different processes in eukaryotic cells which rely on the organization and dynamics of the cytoskeleton.. In plant cells microtubules and actin filaments form the backbone of the cytoskeleton. These structures support cytoplasmic streaming cell wall organization and tracking of cellular material to and from the plasma membrane. To better understand the underlying mechanisms of cytoskeletal organization dynamics and coordination frameworks for the quantification are needed. While this is fairly well established for the microtubules the actin cytoskeleton has remained difficult to study due to its highly dynamic behaviour. One aim of this thesis was therefore to provide an automated framework to quantify and describe actin organization and dynamics. We used ...