Engineering of enzymatic redox cascades involving oxygenases Cascade reactions are not only of interest to chemists and biotechnologists but also to life in general because every metabolic reaction resembles a cascade reaction. This principle of substrate intermediate channeling was only adapted by scientists. That way especially one pot reactions became very attractive as for this no isolation of intermediates is necessary. Furthermore unstable or toxic intermediates are only produced in low amounts and directly transformed in situ. In this PhD thesis two previously established cascade reactions were subject of further optimization. In the first part a cascade reaction established in a DFG funded project (Bo1862 6 1)in cooperation with the Vienna Technical University (Austria) for the production of chiral lactones was further optimized and extended. Therefore on the one hand the genes encoding the needed enzymes were cloned for co expression into a single plasmid in different arrangements to be expressed in pseudo operon mode with the aim to lower the metabolic burden of the cascade host cell. One out of the welve created constructs showed a reasonable activity of 15.3 ± 1.2 U · gCDW 1. On the other hand this cascade reaction was aimed to be extended by the use of a hydroxylating enzyme to enable the use of limonene as renewable and chiral precursor for the proposed production of chiral polymers. Therefore the feasibility of cytochrome P450 monooxygenases was studied. These turned out to be not applicable due to their bad ... Biokatalyse Synthetische Biologie Immobilisierung Prozesstechnik Enzyme Reaktionskaskade Enzymidentifizierung Molecular Modelling Biocatalysis Synthetic Biology Immobilization Process engineering Enzyme identification