Epithelial cell culture protocols Machine generated contents note: 1 Human Lens Epithelial Cell Culture Nobuhiro Ibaraki 1 2 Human Airway Epithelial Cell Culture Mutsuo Yamaya Masayoshi Hosoda Tomoko Suzuki Norihiro Yamada and Hidetada Sasaki 7 3 Rat Gastric Mucosal Epithelial Cell Culture Yoshitaka Konda and Tsutomu Chiba17 4 Thymic Epithelial Cell Culture Carsten Rpke 27 5 Bile Duct Epithelial Cell Culture Alphonse E. Sirica 37 6 Liver Epithelial Cell Culture Rolf Gebhardt 53 7 Pulmonary Epithelial Cell Culture Ben Forbes65 8 Prostate Epithelial Cell Isolation and Culture David L. Hudson77 9 A Bovine Mammary Endothelial Epithelial Cell Culture Model of the Blood Milk Barrier Albert Guidry and Celia OBrien85 10 The Blood CSF Barrier in Culture: Development of a Primary Culture and Transepithelial Transport Model for Choroidal Epithelial Cells Wei Zheng and Qiuqu Zhao99 11 An In Vitro Model of Differentiated Human Airway Epithelia: Methods for Establishing Primary Cultures Philip H. Karp Thomas 0. Moninger S. Pary Weber Tamara S. Nesselhauf Janice L. Launspach Joseph Zabner and Michael J. Welsh115 12 Primary Mouse Keratinocyte Culture Kairbaan Hodivala Dilke139 13 Analyzing Apoptosis in Cultured Epithelial Cells Andrew P. Gilmore and Charles H. Streuli145 14 Keratins as Markers of Epithelial Cells David L. Hudson157 15 Epithelial Cell Integrins Joachim Rychly 169 16 Isolation Cultivation and Differentiation of Normal Human Epidermal Keratinocytes in Serum Free Medium Sebastian Zellmer and Dieter Reissig 179 17 Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces Norio Kumagai185 18 Transplantation of Cultured Epithelial Cells Minoru Ueda Yukio Sumi Yoshitaka Hibino and Ken lchiro Hata197 19 An Outgrowth Culture System of Normal Human Epithelium: An In Vitro Model to Study the Coordination of Cellular Migration and Proliferation Tohru Masui 213 20 Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract Jie Shen and Vincent H. L. Lee217 21 Applications of Epithelial Cell Culture in Studies of Drug Transport Staffan Tavelin Johan Grasj6 Jan Taipalensuu Goran Ocklind and Per Artursson233 22 X Ray Microanalysis of Epithelial Cells in Culture Godfried M. Roomans273 23 ENU Mutagenesis of Rat Mammary Epithelial Cells George Stoica 291 24 ENU lnduced Ovarian Cancer George Stoica 305 25 Measurement of Membrane Capacitance in Epithelial Monolayers Carol A. Bertrand and Ulrich Hopfer315 26 Assessing Epithelial Cell Confluence by Spectroscopy Simon A. Lewis329 27 Co Cultivation of Liver Epithelial Cells with Hepatocytes Rolf Gebhardt337 28 Co Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium Wee Soo Shin Chieko Hemmi and Teruhiko Toyo oka347 29 Establishing Epithelial Immune Cell Co Cultures: Effects on Epithelial Ion Transport and Permeability Derek M. McKay and Mary H. Perdue359 30 Explant Cultures of Embryonic Epithelium: Analysis of Mesenchymal Signals Carin Sahlberg Tuija Mustonen and Irma Thesleff373 31 Bacterial Interactions with Host Epithelium In Vitro Nicola Jones Mary H. Perdue Philip M. Sherman and Derek M. McKay383 Index4 0 1