Calcium techniques : a laboratory manual This laboratory manual provides step by step protocols for studying many facets of Ca2+ signaling as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent luminescent and genetically encoded Ca2+ probes in conjunction with state of the art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+ mobilizing messengers (IP3 cADPR and NAADP). Techniques to modulate and suppress intra and intercellular signals are also provided. Each protocol is complete with a list of required materials detailed recipes for media and reagents and troubleshooting advice