PCR protocols : a guide to methods and applications The broad utility of the polymerase chain reaction method is now within the reach of every researcher. Designed for use at the laboratory bench this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures with advice on primer design. All of the techniques described from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA are tested current and supplemented with helpful notes and illustrations