dna sequencer 

Statements about this table:
dna sequencer pacbio have the lowest accuracy at 90%
hiseq 2000 and 454 gsflx be 2 of the dna sequencer with the a very high accuracy at 99.9%
both pacbio and ion torrent pgm take 2 hour time per run
solidv4 be the cheapest cost per mb at 0.13usd
sequencer
ion torrent pgm
454 gs flx
hiseq 2000
solidv4
pacbio
sanger 3730xl
manufacturer
ion torrent (life technologies)
454 life sciences (roche)
illumina
applied biosystems (life technologies)
pacific biosciences
applied biosystems (life technologies)
sequencing chemistry
ion semiconductor sequencing
pyrosequencing
polymerase - based sequence - by - synthesis
ligation - based sequencing
phospholinked fluorescent nucleotides
dideoxy chain termination
amplification approach
emulsion pcr
emulsion pcr
bridge amplification
emulsion pcr
single - molecule , no amplification
pcr
data output per run
100 - 200 mb
0.7 gb
600 gb
120 gb
100 - 500 mb
1.9∼84 kb
accuracy
99%
99.9%
99.9%
99.94%
90%
99.999%
sequencer
ion torrent pgm
454 gs flx
hiseq 2000
solidv4
pacbio
sanger 3730xl
time per run
2 hours
24 hours
3 - 10 days
7 - 14 days
2 hours
20 minutes - 3 hours
read length
200 - 400 bp
700 bp
100x100 bp paired end
50x50 bp paired end
1500 - 5000 bp (avg)
400 - 900 bp
cost per run
350 usd
7000 usd
6000 usd (30x human genome)
4000 usd
300 usd
4 usd (single read / reaction)
cost per mb
1.00 usd
10 usd
0.07 usd
0.13 usd
2.00 usd
2400 usd