The next match which was billed as a "Battle for Brand Supremacy", was between SmackDown's Batista and Raw's Umaga. Early in the match, both Batista and Umaga exchanged blows and Batista threw Umaga outside with a shoulder block. Umaga later delivered a high-impact kick to Batista, which caused him to fall back-first outside the ring from the ring-apron. Hence, Umaga started targeting Batista's injured back. But in the end, when Umaga tried to go for a Samoan Spike, Batista countered the attempt and delivered a spinebuster to Umaga. Batista won the match by pinning Umaga after hitting him with his Batista Bomb. The fourth match for the event featured Chavo Guerrero defending his ECW Championship against Kane. Kane surprised Chavo by emerging from underneath the ring instead of from the entrance stage. In the second shortest match in WrestleMania history, Kane instantly pinned Chavo after a Chokeslam and won the ECW Championship in eight seconds.
Ric Flair put his career on the line next against Shawn Michaels. At the start of the match, both superstars engaged in a series of counters, and then Flair shoved Michaels in a corner, making "Old Yeller" comments to him. In retaliation, Michaels slapped Flair in the face, which caused him to start bleeding from the mouth. Later, Flair performed a crossbody onto Michaels from the top rope for a two count. Then, in a very dangerous spot, when Flair was on the outside, Michaels performed an Asai moonsault onto Flair, but missed and landed hard on the Raw announcers' table rib-first. With Flair later on outside the ring again, Michaels successfully attempted another moonsault from the top-rope onto Flair. Back in the ring, Michaels tried to go for his Sweet Chin Music, but stopped in the process and Flair capitalized by trapping him in his figure four leglock. Afterwards, Michaels finally delivered a Sweet Chin Music to Flair, but the latter kicked out to the pinfall attempt. Michaels then, trapped Flair in his modified figure four leglock, but Flair delivered a thumb to the eye to Michaels to break the submission. As Flair was delivering chops to Michaels, the latter hit a second Sweet Chin Music. After getting up on his feet with a worried face, Michaels said to Flair "I'm sorry, I love you", before nailing a final Sweet Chin Music and thus pinning Flair to end his 36-year long wrestling career. After the match, Michaels left quickly and Flair got a standing ovation from the crowd. An emotional Flair embraced his family at ringside and then, as he proceeded to go backstage, thanked the crowd for their support.
The sixth match was the Playboy BunnyMania Lumberjack match, in which Maria and Ashley faced Beth Phoenix and Melina, who were accompanied to the ring by Santino Marella. Snoop Dogg served as the official "Master of Ceremonies" for this match. Due to some technical difficulties, the lights at Citrus Bowl temporarily went out during the match. Near the end, a pin attempt by Maria was prevented when Santino pulled Maria's leg. In response, Raw commentator Jerry Lawler approached and knocked Marella down with a punch. With a Fisherman suplex, Phoenix pinned Maria to win the match. After the match, Snoop Dogg clotheslined Marella in the ring and kissed Maria, before leaving with her and Ashley.
Randy Orton defended his WWE Championship against Triple H and John Cena in a Triple Threat match next. For his entrance, Cena had a marching band perform an instrumental version of his theme song ""The Time Is Now"" live. During the match, when Triple H had held Orton in a sleeper hold, Cena picked up both Orton and Triple H for an FU, but Triple H dropped down and low blowed him. Orton dominated the match for some time. One hightlight of the match featured Orton performing a crossbody from the top rope on Cena, while the latter was held on Triple H's shoulders in an electric chair position. Also, Orton delivered a double elevated DDT from the second rope to both Cena and Triple H simultaneously. Orton then tried to RKO Cena, but he countered and threw Orton onto Triple H. Then, Triple H started targeting Orton's legs and using some submissions on him. The match's finish came when Triple H countered Cena's FU into a Pedigree, but when he covered Cena, Orton punted on Triple H's head and himself pinned Cena to retain his WWE Championship.
The next match was the No Disqualification match between The Big Show and Floyd Mayweather, Jr.. Early in the match, Mayweather repeatedly escaped Big Show's grasp and delivered body shots to him. Soon, Mayweather put a sleeper hold on Big Show, but the latter escaped out of it before passing out and stomped on Mayweather's hand. Mayweather and his accomplices tried to "walk out" of the match, but Big Show walked up the ramp and brought Mayweather back in the ring. As Big Show was about to chokeslam Mayweather, one of Mayweather's accomplices struck a steel chair on Big Show's back, and the latter chokeslammed him in retaliation. Capitalizing from this distraction, Mayweather grabbed that chair and hit Big Show multiple times on the head with it. Finally, Mayweather removed his right glove and put on a pair of brass knuckles to hit Big Show in the face. Thus, Big Show was knocked out as he could not answer the referee's ten count and Mayweather was declared the winner.
The main event and last match of the night was Edge putting his World Heavyweight Championship on the line against the number one contender The Undertaker.
As the second WrestleMania to be held outdoors the whole time, WWE chairman Vince McMahon early on said that the show will go on, no matter what the weather is. In the March 2008 issue of WWE Magazine, WWE set designer Jason Robinson revealed that a steel rig with a tarpaulin roof would be built above the ring itself to prevent rain. In that same issue, an initial design of the ring setup was revealed showing a bigger rig surrounding the tarpaulin rig, with lighting and two giant screens attached. The bigger rig was dropped from the final design with the lighting and video screens now featuring on the tarpaulin rig as well as the sound system. During an interview, WWE production manager Brian Petree mentioned that video reinforcement should prevent anyone from being obstructed by the steel structure. Up to seven generators were used to power up the show.
The set design for the entrance stage was at the north end of the stadium and consisted of another steel structure with various video screens hanging from it. The steel beams for the structure were custom built in Belgium and shipped over to Orlando. According to WWE Magazine, the amount of pyrotechnics used would be ten times that of the amount used on "Raw". Without the restriction of a roof, the pyrotechnics for the show shot as high as 2,000 ft as compared to WrestleMania 23's height of 150 ft. The fireworks were set off from boats on one of the lakes nearby the stadium. WWE has been said to have spent an estimated $300,000 on the fireworks alone. After the show, WWE was criticized for a malfunction in the pyrotechnics during The Undertaker's victory celebration. During the celebration, a hot cable for pyrotechnics was sent flying into audience members in the upper seating bowl of the stadium, leaving 45 injured with some hospitalized. The accident was apparently due to a cable which fireworks were travelling across snapping, thus resulting in the fireworks exploding into the top rows of the upper bowl of the stadium. WWE's corporate website released a statement afterwards stating that they will investigate the incident.