translate English to French: For the amplification of a 900 bp toxin DNA fragment, the following primer sequences are required: 5'gaa cga aat aat tta tat gt3' (LinU), and 5'ttt gat tgt tac agt cat3' (LinD)
40 cycles : 4. de dénaturation, 1 minute, 94°C 5. d'hybridation, 1.5 minute, 43°C 6. d'élongation, 1.5 minute, 72°C 7. 8. suivi de : 9. un maintien indéfini à 4oC