translate English to French: Despite die technical difficulties encountered, freeze substitution was effective in preserving the overall shape of the zoospores and membranous structures, including the plasma membrane, peripheral cisternae, and the water-expulsion vacuole.
Cette méthode améliore la conservation de diverses structures vésiculaires et vacuolaires, en particulier les cisternes périphériques et les vacuoles d'expulsion de l'eau.