Authors: Christina H. Wegner | Sebastian Mathis Eming | Brigitte Walla | Daniel Bischoff | Dirk Weuster‐Botz | Jürgen Hubbuch
Source: Frontiers in Bioengineering and Biotechnology (article, 2024-05-15)
Field: Materials Science — Materials Chemistry

Spectroscopic insights into multi-phase protein crystallization in complex lysate using Raman spectroscopy and a particle-free bypass Protein crystallization as opposed to well-established chromatography processes has the benefits to reduce production costs while reaching a comparable high purity. However, monitoring crystallization processes remains a challenge as the produced crystals may interfere with analytical measurements. Especially for capturing proteins from complex feedstock containing various impurities, establishing reliable process analytical technology (PAT) to monitor protein crystallization processes can be complicated. In heterogeneous mixtures, important product characteristics can be found by multivariate analysis and chemometrics, thus contributing to the development of a thorough process understanding. In this project, an analytical set-up is established combining offline analytics, on-line ultraviolet visible light (UV/Vis) spectroscopy, and in-line Raman spectroscopy to monitor a stirred-batch crystallization process with multiple phases and species being present. As an example process, the enzyme <i>Lactobacillus kefir</i> alcohol dehydrogenase (L<i>k</i>ADH) was crystallized from clarified Escherichia coli (<i>E. coli</i>) lysate on a 300 mL scale in five distinct experiments, with the experimental conditions changing in terms of the initial lysate solution preparation method and precipitant concentration. Since UV/Vis spectroscopy is sensitive to particles, a cross-flow filtration (cross-flow filtration)-based bypass enabled the on-line analysis of the liquid phase providing information on the lysate composition regarding the nucleic acid to protein ratio. A principal component analysis (PCA) of <i>in situ</i> Raman spectra supported the identification of spectra and wavenumber ranges associated with productspecific information and revealed that the experiments followed a comparable, spectral trend when crystals were present. Based on preprocessed Raman spectra, a partial least squares (PLS) regression model was optimized to monitor the target molecule concentration in real-time. The off-line sample analysis provided information on the crystal number and crystal geometry by automated image analysis as well as the concentration of <i>Lk</i>ADH and host cell proteins (HCPs) In spite of a complex lysate suspension containing scattering crystals and various impurities, it was possible to monitor the target molecule concentration in a heterogeneous, multi-phase process using spectroscopic methods. With the presented analytical set-up of off-line, particle-sensitive on-line, and in-line analyzers, a crystallization capture process can be characterized better in terms of the geometry, yield, and purity of the crystals. Raman spectroscopy ; Crystallization ; Lysis ; Particle (ecology) ; Phase (matter) ; Materials science ; Chemistry ; Chemical engineering ; Crystallography ; Biochemistry ; Physics ; Biology ; Organic chemistry ; Optics Enzyme Structure and Function ; Crystallization and Solubility Studies ; Spectroscopy Techniques in Biomedical and Chemical Research