Authors: Bojidarka Ivanova
Source: Advances in Analytic Science (article, 2024-12-25)
Field: Chemistry — Spectroscopy

Stochastic dynamics mass spectrometric structural analysis of nucleotides &lt;span lang="EN-US"&gt;The paper deals with innovative equations tackling exactly stochastic dynamics mass spectrometric experimental variable intensity of peak per span of scan time. They overcome limitations of classical methods for semi-quantifying analytes developed, so far, and exactly grasp observable variables and their fluctuations; thus, succeeding in determining analytes reliably both quantitatively and 3D structurally via soft ionization mass spectrometry. &lt;/span&gt;&lt;span lang="EN-US"&gt;Given the&lt;/span&gt;&lt;span lang="EN-US"&gt; paper’s goal of illustrating their crucial &lt;/span&gt;&lt;span lang="EN-US"&gt;e&lt;/span&gt;&lt;span lang="EN-US"&gt;ffect on mass spectrometric methodology as&lt;/span&gt;&lt;span lang="EN-US"&gt; an&lt;/span&gt;&lt;span lang="EN-US"&gt; irreplaceable approach to structurally analyse species, this study offers stochastic dynamics&lt;/span&gt;&lt;span lang="EN-US"&gt;-&lt;/span&gt;&lt;span lang="EN-US"&gt;based analysis of nucleotides. The major contribution &lt;/span&gt;&lt;span lang="EN-US"&gt;is&lt;/span&gt;&lt;span lang="EN-US"&gt; providing empirical justification of aspects of structural mass spectrometry analysing uridines and pseudouridines. The virtual identity of fragmentation patterns causes significant analytical challenge&lt;/span&gt;&lt;span lang="EN-US"&gt;s&lt;/span&gt;&lt;span lang="EN-US"&gt;. The same is true for methyl-substituted guanosines which are often determined as mixtures. There &lt;/span&gt;&lt;span lang="EN-US"&gt;are&lt;/span&gt;&lt;span lang="EN-US"&gt; used ultra-high resolution electrospray ionization mass spectrometry, high accuracy computational static and molecular dynamics methods, and chemometrics. The study discusses controversial aspects of classical techniques. It illustrates how the innovative equations resolve disputable problems of structural analysis of nucleotides. It supports advanced formulas for achieving superior performances. There are obtained coefficients of linear correlation &lt;/span&gt;&lt;span lang="DE"&gt;|&lt;/span&gt;&lt;em&gt;&lt;span lang="EN-US"&gt;r&lt;/span&gt;&lt;/em&gt;&lt;span lang="DE"&gt;|&lt;/span&gt;&lt;span lang="EN-US"&gt; = 0.9994–0.99923 determining N1-methyl-pseudouridine modified diphosphate compar&lt;/span&gt;&lt;span lang="EN-US"&gt;ed&lt;/span&gt;&lt;span lang="EN-US"&gt; with 5-methyluridine diphosphate N-acetylglucosamine. There are determined N&lt;sup&gt;2&lt;/sup&gt;,N&lt;sup&gt;2&lt;/sup&gt;-dimethylguanosine, uridine, pseudouridine, 5-methyl-uridine, 1-methyl-pseudouridine, 5,6-dihydrothymidine, galactosyl-queuosine, mannosyl-queuosine, adenosine, 2’-O-methyl-5-hydroxymethyl&lt;/span&gt;&lt;span lang="EN-US"&gt;cytidine, uridine triphosphate, thymidine diphosphate N-acetylglucosamine, 5-methyluridine diphosphate N-acetylglucosamine, and 7-methylguanosine-5’-phosphate modified derivative, respectively.&lt;/span&gt; Span (engineering) ; Chemistry ; Mathematics ; Engineering ; Structural engineering Mass Spectrometry Techniques and Applications ; Analytical Chemistry and Chromatography ; Metabolomics and Mass Spectrometry Studies