Study of the mainstream cigarette smoke aerosols by Fourier transform ion cyclotron resonance mass spectrometry coupled to laser/desorption and electrospray ionization - Additional insights on the heteroaromatic components. 
The chemical composition of the particulate phase of cigarette smoke inhaled by the active smoker is still poorly known in spite of its importance from a health point of view.  A non-targeted approach is applied to cigarette smoke particles collected on a quartz filter to obtain an as complete as possible description of this complex mixture.  A home-made smoking machine including devices for volatile organic compounds (VOCs) and particle sampling was used.  The validation of the cigarette smoking and cigarette smoke collection procedures was conducted by the quantification of some compounds by gas chromatography/mass spectrometry (GC/MS).  The particles were investigated by Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) directly after their collection on quartz filters by laser/desorption ionization (LDI) or after extraction with CH2 Cl2 by electrospray ionization (ESI).  The determination of the benzene, toluene, ethylbenzene and xylenes (from 2 to 35 μg/cigarette) and nicotine (0.68 ± 0.05 mg/cigarette) validated the used sampling method.  The complementarity of the LDI and ESI sources for the cigarette smoke analysis was established.  The ESI analyses evidenced polar compounds and components with a pyridine group and LDI ensured the detection of poly-condensed heteroaromatic species.  Finally, this methodology was employed to characterize particles from cigarettes with or without flavoring additives.  Some insights into the composition of cigarette smoke inhaled by active smokers have been obtained.  The ~1750 observed features revealed the huge complexity of cigarette smoke particles and the diversity of the possible associated health issues.  Both heteroaromatic and highly oxygenated compounds produced by combustion and pyrolysis have been highlighted.