Introduction {#S1}
============

Aurora Kinase A (Aurora-A) belongs to a family of conserved mitotic Serine/Threonine kinases that have fundamental roles in the regulation of cellular division.  It is found predominantly at the poles of dividing cells, localized to the centrosomes and proximal spindles.  Aurora-A is required for the timely entry into mitosis, centrosome maturation, formation of bipolar spindles, and has been implicated in the breakdown of the nuclear envelope and the completion of cytokinesis ([@R34]; [@R49]).  Moreover, a role for Aurora-A was recently demonstrated in the control of mitochondrial fission during mitosis ([@R22]).  Its protein stability and activity are tightly coupled to the stages of the cell cycle, peaking at the G2/M transition and suppressed at anaphase through protein degradation that is mediated by the Anaphase promoting complex ([@R20]; [@R29]).  Numerous interacting factors such as AJUBA, BORA, NEDD9, PAK, PLK1 and TPX2 have been identified as important for Aurora-A's activation and cellular localization ([@R21]).  Downstream targets of Aurora-A are diverse and growing ([@R23]; [@R24]).  Of these, Aurora-A can phosphorylate the cell cycle regulator CDC25B and thereby affect the centrosomal redistribution of CDK1--Cyclin B1 complexes to promote mitotic entry ([@R12]).  Aurora-A can also activate the tubulin deacetylase, HDAC6, to regulate cilia disassembly ([@R38]), and phosphorylate NDEL1 to promote neurite extension ([@R35]).  Thus, Aurora-A has cellular functions that go beyond its ability to regulate cell division. 

Aurora-A is also a proto-oncogene that is frequently amplified or overexpressed in epithelial cancers including squamous cell carcinomas (SCCs) ([@R7]; [@R48]).  Aurora-A was further identified as a low penetrance cancer susceptibility gene in both humans and mice ([@R13]; [@R14]).  Ectopic Aurora-A expression can transform immortalized rodent cell lines *in vitro* and promote their growth in immunocompromised mice ([@R5]; [@R52]).  We have previously shown in genetically engineered cancer mouse models that Aurora-A can co-operate with a classical skin chemical carcinogenesis protocol or with the expression of a gain-of-function *p53^R172H^* mutant allele to promote the formation of metastasis-prone skin SCCs ([@R47]; [@R48]).  Due to its role in cell division, Aurora-A may be an attractive target for therapeutic intervention in skin cancers.  Currently, there are several small molecule inhibitors to Aurora Kinase A and B in clinical trials ([@R18]).  However, the long-term off-tumor effects of these inhibitors remain undefined, especially in skin.  Inhibition of Aurora-A activity in cultured cells results in mitotic abnormalities, misalignment of chromosomes, and growth suppression ([@R3]).  Recently, three different laboratories have described the global deletion of *Aurora-A* in mice ([@R9]; [@R30]; [@R42]).  In these reports, deletion of *Aurora-A* resulted in embryonic death prior to implantation ([@R9]; [@R30]; [@R42]).  From these studies, it is clear that Aurora-A is essential for life, but the early lethality of these mice precluded the study of Aurora-A's function in tissue specific development or homeostasis.  In the present study, we examined the consequences of keratinocyte-specific deletion of *Aurora-A* to determine its role in the formation and maintenance of skin epithelia. 

RESULTS {#S2}
=======

Skin-specific deletion of *Aurora-A* {#S3}
------------------------------------

To delete Aurora-A in skin, we interbred floxed *Aurora-A* mice ([@R42]) with a constitutively active Cre recombinase expressed from a truncated Keratin 14 promoter (K14.Cre) ([@R10]).  This Cre deleter strain begins to express Cre in epithelial tissues at E10.5 and displays Cre activity more uniformly by E12.5 as shown by the activation of the Rosa26 β-galactosidase Cre-reporter ([Supplementary Figure 1a](#SD1){ref-type="supplementary-material"}). * Aurora-A^fl/+^*; *K14.Cre* mice appeared phenotypically normal.  In contrast, *Aurora-A^fl/fl^*; *K14.Cre* mice died shortly after birth, and had obvious skin related abnormalities including fragile, translucent and stretched skin, the failure of eyelids and ears to form normally, fused fingers, and the absence of whisker follicles ([Figure 1a](#F1){ref-type="fig"}).  Moreover, toluidine penetration assays in E18.5 embryos revealed defects in skin barrier function, which were more pronounced on the ventral side of the embryos ([Figure 1b](#F1){ref-type="fig"}).  Detection of the deleted floxed allele was evident in the skin of both *Aurora-A^fl/+^*; *K14.Cre* and *Aurora-A^fl/fl^*; *K14.Cre* newborn mice ([Fig 1c](#F1){ref-type="fig"} and [Supplemental Figure 1b](#SD1){ref-type="supplementary-material"}).  Deletion of the floxed allele was associated with reduced Aurora-A mRNA levels in whole skin ([Supplemental Figure 2a](#SD1){ref-type="supplementary-material"}) and the absence of detectable Aurora-A protein in actively dividing keratinocytes of *Aurora-A^fl/fl^*; *K14.Cre* embryos ([Supplemental Figure 2b](#SD1){ref-type="supplementary-material"}).  Collectively, these observations indicate that a defective skin barrier, cause by epidermal Aurora-A deficiency, most likely led to desiccation and perinatal lethality of *Aurora-A^fl/fl^*; *K14.Cre* newborn mice. 

Histological evaluation of the skin of *Aurora-A^fl/fl^*; *K14.Cre* mice at E16.5 and older showed a thin epidermis, and the absence of mature hair follicles ([Figure 2](#F2){ref-type="fig"}).  We focused on the interfollicular keratinocyte compartment for the remainder of this study.  The nuclei of the *Aurora-A^−/−^* keratinocytes appeared enlarged with multiple nucleoli.  Marker analysis revealed the expression of Krt1 and 10 in the suprabasal compartment, whereas Krt14 and Krt5, and the stress Keratin, Krt6 were evident in the basal layer of *Aurora-A^fl/fl^*; *K14.Cre* dorsal skin ([Figure 2](#F2){ref-type="fig"}).  The late differentiation markers, Loricrin and Filaggrin, were also detectable in the outermost layer of *Aurora-A^−/−^* epidermis.  However, the expression of Filaggrin was markedly reduced or sporadic ([Figure 2](#F2){ref-type="fig"}).  A similar pattern of expression was observed for Krt1 on the ventral side of E17.5 *Aurora-A^fl/fl^*; *K14.Cre* embryos, whereas Loricrin and Filaggrin expression was markedly reduced or absent ([Supplementary Figure 3](#SD1){ref-type="supplementary-material"}).  The lack of Loricrin and Filaggrin expression on the ventral side likely contributed to a more severe impairment of barrier function in *Aurora-A^fl/fl^*; *K14.Cre* embryos (see [Figure 1b](#F1){ref-type="fig"}).  Both Collagen IV and integrinα6 were found at the epidermal/dermal junction of Aurora-A deficient epidermis, and we observed an expression pattern similar to wildtype (WT) for the adherent junction proteins Zo-1, Desmoglein 1/2 and Desmocolin3 (data not shown).  Thus, Aurora-A deficiency did not disrupt the integrity of the basement membrane, or cellular junctions of the epidermis. 

Delayed stratification of *Aurora-A^−/−^* skin epithelia {#S4}
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The phenotypic presentation and abnormal epidermal architecture of *Aurora-A^fl/fl^*; *K14.Cre* prenatal mice suggested to us that Aurora-A deficiency may impair the formation of the stratified layers of the skin.  We therefore examined the embryonic stages between E12--14, when the epithelium stratifies to create the suprabasal compartment ([@R25]).  Unlike WT cells, *Aurora-A^−/−^* epidermal progenitors were delayed in forming a suprabasal layer in both the ventral and dorsal skin, as evident by histological evaluation and the late appearance of Krt1 expressing cells ([Figure 3a](#F3){ref-type="fig"}).  Because suppression of proliferation could account for a delay in the emergence of suprabasal keratinocytes, we examined the S and M phases of the cell cycle in developing skin.  At E13.5 similar numbers of keratinocytes in both WT and *Aurora-A^−/−^* epidermis were positive for BromodeoxyUridine (BrdU) incorporation indicating that Aurora-A deficiency did not alter the ability of keratinocytes to enter S-phase ([Figure 3c](#F3){ref-type="fig"}).  Next, we stained for phospho-Histone H3 to determine the relative number of cells in mitosis ([@R45]).  As shown in [Figure 3b-c](#F3){ref-type="fig"} for WT epidermis, only a few basal and suprabasal layer cells were positive for phospho-Histone H3, consistent with a short duration spent tranversing M-phase ([@R11]).  In contrast, a much higher number of positive cells were found in *Aurora-A^−/−^* versus WT skin (a 3 and 6-fold increase in the dorsal and ventral epidermis, respectively).  Furthermore, mitotic cells in *Aurora-A^−/−^* epidermis lacked a fully formed nuclear envelope as shown by a diffused staining pattern for Lamin A/C, a nuclear membrane structural component ([@R33]) ([Supplementary Figure 4](#SD1){ref-type="supplementary-material"}).  As the nuclear envelope is disassembled prior to metaphase in mitosis ([@R33]), Aurora-A deficient keratinocytes appear to stall at pro-metaphase of the cell cycle. 

The outcome of a stalled mitosis in *Aurora-A^−/−^* keratinocytes {#S5}
-----------------------------------------------------------------

Cells that are stalled in mitosis can undergo cell death or escape without completion of cytokinesis by a process that is called mitotic slippage ([@R39]).  To determine if apoptosis was a consequence of deleting Aurora-A in keratinocytes, we stained *Aurora-A^−/−^* epidermis for the apoptotic marker, active Caspase 3.  Unlike WT skin, active Caspase 3 was readily detectable in *Aurora-A^−/−^* epidermis.  Its detection peaked at E13.5, which coincided with the commencement of stratification ([Figure 4](#F4){ref-type="fig"}).  Another outcome of a delayed mitosis is the failure to generate two daughter cells, leaving the cell with twice the normal complement of genetic material.  To test this possibility, we enumerated centromeres by staining paraffin sections of embryonic skin with a pan-centromere FISH probe.  Increased numbers of centromeres were found in *Aurora-A^−/−^* epidermis relative to the nuclei found in WT epidermis or in the dermis of *Aurora-A^flfl^; K14.Cre* mice that remained WT for Aurora-A ([Figure 5](#F5){ref-type="fig"}).  Moreover, cells in metaphase or anaphase were rarely observed by histology or by staining with Aurora-B, which localizes to the midzone or anaphase bridges during mitosis ([@R41]) (data not shown).  From these analyses, we conclude that *Aurora-A^−/−^* keratinocytes gave rise to polyploid cells. 

Centrosome abnormalities in *Aurora-A^−/−^* keratinocytes {#S6}
---------------------------------------------------------

Aurora-A promotes the maturation of centrosomes and formation of bi-polar spindles in preparation for cell division ([@R49]).  Therefore, we examined how Aurora-A deficiency affected centrosome function in the developing epidermis.  In WT keratinocytes at E16.5 and older, the centrosomes were predominately found apically ([Figure 6A](#F6){ref-type="fig"}, arrows).  This orientation was altered in *Aurora-A^−/−^* epidermis, with the position of the centrosomes becoming random and located either apically, basolaterally or centrally ([Figure 6a](#F6){ref-type="fig"}, arrows, and see [Supplementary Figure 5](#SD1){ref-type="supplementary-material"}).  As shown in [Figure 6b](#F6){ref-type="fig"}, there were multipolar structures or centrosome clusters in *Aurora-A^−/−^* epidermis, reminiscent of spindles anomalies observed in cells with extra centrosomes ([@R15]) or those treated with Aurora-A inhibitors, and in Drosophila mutants of Aurora-A ([@R17]; [@R32]).  Since the proper orientation of centrosomes is required for the formation of bi-polar spindles in preparation for metaphase, we next examined the microtubule network in *Aurora-A^−/−^* epidermis.  In actively dividing WT cells, microtubules are organized between two opposing nucleating poles (i.e., the centrosomes) whose orientation to the basement membrane determines the plane of cell division ([@R16]).  A basal cell that forms a bi-polar spindle perpendicular to the basement membrane will divide asymmetrically and give rise to two cells, a suprabasal and a basal keratinocyte ([Figure 6c](#F6){ref-type="fig"}) ([@R26]; [@R44]).  In *Aurora-A^−/−^* keratinocytes, basal cells with bi-polar spindles were rarely observed at E13.5 or at later embryonic stages.  In dividing cells identified by phospho-Histone H3 expression, the microtubule network appeared disorganized ([Figure 6c](#F6){ref-type="fig"}).  Taken together, these data indicate that abnormal centrosome function induced by the deletion of *Aurora-A* leads to a defective cellular division and the mislocation of centrosomes following mitosis of basal cells. 

To determine if the effects of Aurora-A deficiency were similar in post-natal skin, Aurora-A floxed mice were crossed with the Tamoxifen-inducible Cre deleter strain, K14.CreER ([@R50]).  This Cre-fusion shows robust deletion of floxed alleles in keratinocytes both *in vitro* and *in vivo* after treatment with Tamoxifen (Tam) ([Supplemental Figure 6a](#SD1){ref-type="supplementary-material"}).  Similar to *Aurora-A^fl/fl^; K14.Cre* embryos, Aurora-A was not detectable in dividing keratinocytes present in the skin of Tamoxifen treated *Aurora-A^fl/fl^; K14.CreER* mice ([Supplemental Figure 6b](#SD1){ref-type="supplementary-material"}).  Surprisingly, the deletion of *Aurora-A* in postnatal epidermis resulted in no obvious gross skin abnormalities (e.g., hair loss or skin erosions) 3 months following the induction of Cre activity by Tamoxifen treatment (n=6 and data not shown).  We were able to detect cells that were positive for phospho-Histone H3 or active Caspase 3 in *Aurora^fl/fl^; K14.CreER* epidermis 10 days after the induction of Cre ([Supplementary Figure 7](#SD1){ref-type="supplementary-material"}); however, adult epidermis appears to be reasonably tolerant to the deletion of Aurora-A.

DISCUSSION {#S7}
==========

In the present study, we utilized a conditional deletion strategy to demonstrate that the expression of Aurora-A is required for normal skin development.  The deletion of *Aurora-A* in embryonic basal keratinocytes resulted in defective centrosome maturation, which markedly impaired the ability of these cells to divide and stratify.  Consequently, the initiation of stratification and differentiation was delayed in the developing *Aurora-A^−/−^* epidermis, resulting in impaired barrier function and perinatal death.  Previous studies have shown that barrier formation begins on the dorsal epidermis and progresses laterally starting at E16 ([@R19]).  The ventral epidermis is last to acquire a competent barrier.  The incorporation and crosslinking of epidermal differentiation proteins such as Loricrin into the cornified envelope coincides with the formation of barrier function ([@R43]).  In Aurora-A deficient epidermis, barrier formation is defective, leading to a phenotype that is especially prevalent on ventral skin.  Nevertheless, dorsal skin in *Aurora-A^flfl^; K14.Cre* embryos was able to express terminal differentiation proteins and form a partial barrier, whereas the ventral epidermis was not.  How dorsal skin is able to form two layers and undergo differentiation in light of an obvious cell cycle defect in basal keratinocytes is unclear.  It is possible that incomplete Cre-recombination of both floxed Aurora-A alleles between E12.5--13.5 or residual Aurora-A mRNA allowed enough basal progenitors to undergo at least one round of cellular division.  Alternatively, basal delamination of non-mitotic cells may contribute to the formation of the first suprabasal layer in *Aurora-A^fl/fl^; K14.Cre* skin ([@R1]; [@R8]; [@R28]; [@R31]; [@R37]). 

The phenotypic consequence of deleting *Aurora-A* in mouse keratinocytes is reminiscent of the effects of expression of Aurora-A mutants in Drosophila ([@R4]; [@R17]).  Drosophila mutations in Aurora-A result in lower expression of the mutant protein compared to WT flies.  The most penetrant of these mutants induced monopolar spindles in dividing cells, delays in mitosis, and polyploidy ([@R4]; [@R17]).  Invariably, the failure of centrosomes to separate prior to metaphase can explain the effects of suppressing Aurora-A levels in flies.  The deletion of *Aurora-A* in the developing mouse skin mirrored the most severe of the Drosophila mutants, resulting in centrosomal related defects.  Additionally, the centrosomes in *Aurora-A^−/−^* basal cells appeared to lose the predominant apical placement seen in WT keratinocytes ([@R27]) and were found distributed more randomly.  The position of centrosomes, which determines the axis of cell division during mitosis, is set during metaphase in basal keratinocytes ([@R2]; [@R36]).  In the stages that precede metaphase, centrosomes can be found at angles between parallel to perpendicular (0--90°) relative to the basement membrane ([@R36]).  The observed locations of the centrosomes in *Aurora-A^−/−^* keratinocytes may be a consequence of these cells stalling in pro-metaphase, undergoing mitotic slippage, and possibly re-entering the cell cycle. 

Surprisingly, the post-natal deletion of *Aurora-A* resulted in no gross skin defects, suggesting that inhibition of Aurora-A activity may be well tolerated by adult skin, and represents a clear distinction to the effects observed on the prenatal epidermis as we show in this study, or seen in early stage embryos ([@R9]; [@R30]; [@R42]).  Our observations are also consistent with recent clinical trials of Aurora-A small molecule inhibitors that have shown increased phosho-Histone H3 staining in the skin, but the absence of reported skin-related toxicities ([@R6]).  The mechanism that allows adult skin to tolerate *Aurora-A* deletion remains unclear but may depend on the relative higher rate of proliferation observed in embryonic versus adult skin.  In this regard, Aurora-A inhibition would be expected to have a more profound effect on skin cancer cells, which have a higher rate of proliferation ([@R48]), and be more similar to what we observed in embryonic keratinocytes.  More importantly, our data suggest that the use of small molecule inhibitors of Aurora-A may be a safe and viable strategy to treat recurrent or metastasis-prone skin SCCs ([@R15]) if topical delivery of inhibitors can be achieved. 

In summary, we have characterized the cellular and tissue-specific changes associated with Aurora-A deficiency in the developing epidermis.  Aurora-A deficient epidermis may be an ideal model to explore the relationship between the regulation of cell division, migration and differentiation in epidermal homeostasis, and a tool to develop novel approaches to maximize the killing of squamous cell carcinoma cells by targeting mitotic regulators. 

MATERIALS AND METHODS {#S8}
=====================

Generation of Aurora-A deficient epidermis {#S9}
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The previously described floxed Aurora-A(fl) mice ([@R42]), K14.Cre mice ([@R10]), or K14.CreER mice ([@R50]) were interbred to generate Cre positive and heterozygous or homozygous A*urora-A^fl^* mice.  Timed matings were set up at night and the presence of vaginal plugs on the following morning noted as day 0.  Embryos were then collected at days corresponding to different mouse developmental stages.  The University of Colorado Institutional Animal Care and Use Committee approved animal experiments.  Detection of the Cre-recombined floxed Aurora-A allele was performed as previously described ([@R42]).  For experiments using the K14.CreER strain, mice were topically treated with 125 μg of 4 HydroxyTamoxifen (4HOTam) (Sigma) dissolved in ethanol or injected intraperitoneally for 6 days with Tamoxifen (Sigma) (6 mg/40 g body weight) dissolved in sesame oil. 

Dye Penetration and qPCR Assay {#S10}
------------------------------

Dye penetration was tested on E18.5 embryos as previously described ([@R19]).  Briefly, embryos were dehydrated and re-hydrated through a series of methanol washes and subsequently incubated in Phosphate Buffered Saline solution saturated with Toluidine blue (Sigma).  RNA isolation, reversed transcription, and QPCR analysis was performed as previously described ([@R48]). * Aurora-A* mRNA was detected by Taqman assay\# Mn01248177_m1 (Life technologies) and normalized to *Gapdh* (Life technologies) levels. 

Histology, Imuno- and β-galactosidase- staining {#S11}
-----------------------------------------------

Mouse tissues were fixed in 10% neutral buffered formalin (Thermo Fisher Scientific, Pittsburgh, PA), and embedded in Optimal Cutting Temperature compound (Sakura Finetek) for frozen sectioning or processed for paraffin sectioning.  The cut planes were either sagittal at the midline or transverse at the midbody.  For histological evaluation, dewaxed 5 μM sections were stained with hematoxylin and eosin (H&E).  Immunostaining was performed as previously described with modifications ([@R48]).  Briefly, paraffin embedded tissue was dewaxed and antigen retrieval performed using a Tris EDTA ph10 solution containing 0.05% Tween20.  Sections were then blocked and incubated with the following primary antibodies: Keratin (Krt) 1, 5, 10, 14, Loricrin and Filaggrin ([@R40]; [@R51]), Col IV (Abcam), Itα6 (Chemicon), γ-tubulin (Abcam), α-tubulin (Sigma), phospho-Histone H3 (Ser 10) (Cell Signaling), Active Capase 3 (Cell Signaling).  Appropriate secondary antibodies labeled with Alexa488 or 594 were applied and sections mounted on Vectashield Mounting Medium containing DAPI (Vector labs).  Staining detection was performed on a Nikon 90i microscope (Nikon Corporation) using standard techniques.  For BromodeoxyUridine (BrdU) *in vivo* labeling experiments, mice were injected with BrdU (Sigma) as previously described ([@R46]).  Tissue was then harvested after 2hr, fixed, embedded in paraffin, and stained as described above using rat anti-BrdU antibody (Abcam).  Staining for β-galactosidase (X-gal) activity was performed on 10 μm OCT sections as previously described ([@R46]). 

Fluorescence In Situ Hybridization (FISH) {#S12}
-----------------------------------------

FISH Detection and enumeration of mouse centromeres was conducted at the University of Colorado Cancer Center Cytogenetic Core.  Briefly, dewaxed sections were hybridized with SpectrumRed labeled Poseidon All Mouse Centromere Probe (KI-30500, Kreatech Diagnostics, Durham, NC).  Chromatin was counterstained with DAPI (Vectashield Mounting Medium, Vector Laboratories).  Analysis was performed on epifluorescence microscope using single interference filters sets for red (Texas red), blue (DAPI), and triple (blue, red, green) band pass filters.  Number of signals was scored in at least 30 nuclei per selected area in each specimen.  Images were acquired and merged using CytoVision application (Applied Imaging). 

Cell counting and Statistical Analyses {#S13}
--------------------------------------

To enumerate the number of % keratinocytes that were positive for BrdU or phospho-Histone H3, three separate embryos of each genotype were analyzed.  Tissue sections from dorsal and ventral skins at E13.5 were stained for either BrdU incorporation/Krt14 or phospho-Histone H3/Krt14.  The number of BrdU or phospho-Histone H3 positive cells were scored at 200x magnification after counting on average 294 and 575 Krt14 positive keratinocytes/embryo, respectively.  Similar analysis was performed for active Caspase 3/Krt 14 stained tissue sections with an average of 587 Krt 14 positive cells/embryo counted.  Dorsal skin was analyzed in two separate embryos for each embryonic timepoint.  Statistical tests shown in [Figure 5c](#F5){ref-type="fig"} (phosho-Histone H3) were performed using GraphPad Prism v5. 

Supplementary Material {#S17}
======================

This work was supported by grants from NIH: R01CA52607 (DRR) and RO1CA89716 (SS).  We thank Dr. Leila Garcia and the University of Colorado Cancer Center Cytogenetics Core for performing the FISH experiments (P30 CA046934), David Sheneman for technical assistance, and Miss Irene Choi for her assistant in the preparation of this manuscript.  We also acknowledge support from the University of Colorado Skin Disease Research Center Morphology and Phenotyping Core facility (P30 AR057212). 

CONFLICT OF INTEREST

The authors declare no conflict of interest. 

![ Skin abnormalities in mice deficient for epithelial Aurora-A\
(a) Left panels show gross phenotypic presentation of *Aurora-A^fl/fl^*; *K14.Cre* mice at E18.5.  These mice show translucent skin compared to WT controls.  Right panels show a close up of the face and paws.  Note the improperly formed whiskers, nares, eyelids and nails. ( b) Toluidine blue dye penetration assays revealed a defect in barrier function in *Aurora-A^fl/fl^*; *K14.Cre* E18.5 mice. ( c) Tails from newborn mice were digested and PCR performed to detect Cre-mediated recombination of the floxed Aurora-A allele.  Bars= 2.5 mm.](nihms390006f1){#F1}

![ Aurora-A^−/−^ epidermis exhibits a disrupted epidermal architecture\
Histological analysis (H&E) of Aurora-A null dorsal epidermis at E16.5 revealed a thin epidermis and absence of mature hair follicle formation.  However, marker analysis showed the presence of a basal layer by the detection of Krt14 expression, whereas expression of Krt1 marked the presence of the suprabasal compartment.  A similar pattern was observed for Krt10 vs Krt 5 expression (not shown).  The late differentiation markers, Loricrin and Filaggrin were visible in the outermost part of *Aurora-A^−/−^* epidermis.  Bar = 25 μm](nihms390006f2){#F2}

![ Delayed stratification in *Aurora-A^−/−^* epidermis\
(a) Epidermis between E13--14 was examined by histology and marker analysis.  Unlike WT mice that forms two layers at E13.5, *Aurora-A^−/−^* epidermis remains primarily a single layer.  Consistently, few cells expressed the subrabrasal marker Krt1 in the uppermost layer of the skin.  A similar pattern was observed on ventral skin (not shown). ( b) Immunofluorescent detection of phopho Histone H3 (Ser10) in E13.5 skin.  Note the increased number of positive cells found in both dorsal and ventral skins of *Aurora-A^fl/fl^; K14.Cre* mice. ( c) Quantitation of BrdU incorporation and detection of mitotic cells per Krt14 positive keratinocytes.  Data shown is the average counts per three embryos ± standard deviation.  P values are from a t-test.  Bar = 25 μm](nihms390006f3){#F3}

![ Increased apoptosis in Aurora-A deficient epidermis\
Top panel shows the immunodetection of active Caspase 3 in WT and *Aurora-A^−/−^* epidermis at E13.5.  Bar= 25 μm.  Bottom panel shows the quantitation of apoptosis found in developing skin of *Aurora-A^fl/fl^; K14.Cre* or WT embryos (n=2 embryos per genotype and timepoint).  Columns represent average ± standard deviation.  Note the absence of apoptotic cells in WT skin at any embryonic stage.](nihms390006f4){#F4}

![ Aurora-A deficiency induces polyploidy in keratinocytes\
Left panels show the representative FISH detection of a pan mouse centromere probe on 5-micron thick paraffin sections from E16.5 skin of *Aurora^fl/fl^* and *Aurora-A^fl/fl^; K14.Cre* embryos.  Bar=10 μm.  Right, quantification of centromeres.  Mean count ± standard deviation is shown.  Note the consistent number of centromeres in cells found in WT Aurora-A epidermis or the dermis of *Aurora-A^fl/fl^; K14.Cre* mice that do not undergo Cre-recombination.  However, much higher numbers are detected in *Aurora-A^−/−^* epidermis, indicative of polyploidy.](nihms390006f5){#F5}

![ Centrosomal abnormalities in *Aurora-A^−/−^* epidermis\
(a) the location of centrosomes was examined in *Aurora-A^−/−^* E16.5 epidermal skin by immunostaining with γ-tubulin.  Left panels show the apical location of centrosomes (arrows) in basal keratinocytes, relative to the basement membrane.  Right panels show non-apical locations of centrosomes in *Aurora-A^−/−^* epidermis (arrows) Bar= 25 μm (b) Higher magnification of *Aurora-A^−/−^* cells.  Bar= 10 μm (c) Detection of the microtubule network by α-tubulin immunostaining.  Left panel shows formation of a bipolar spindle in WT keratinocytes undergoing mitosis.  Right panels show atypical microtubule formation in Aurora-A deficient basal cells, reminiscent of monopolar spindles.  Bar=5 μm.](nihms390006f6){#F6}
