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FireWire 400 can transfer data between devices at 100, 200, or 400 Mbit/s half-duplex data rates (the actual transfer rates are 98.304, 196.608, and 393.216 Mbit/s, i.e. 12.288, 24.576 and 49.152 megabytes per second respectively). These different transfer modes are commonly referred to as S100, S200, and S400.

Cable length is limited to , although up to 16 cables can be daisy chained using active repéaters, external hubs, or internal hubs often present in FireWire equipment. The S400 standard limits any configuration's maximum cable length to 72 méters. The 6-pin connector is commonly found on desktop computers, and can supply the connected device with power.

The 6-pin powered connector adds power output to support external devices. Typically a device can pull about 7 to 8 watts from the port; however, the voltage varies significantly from different devices. Voltage is specified as unregulated and should nominally be about 25 volts (range 24 to 30). Apple's implementation on laptops is typically related to battery power and can be as low as 9 V and more likely about 12 V.