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Akshay Kumar as Professor Aditya Singh Rajput a.k.a. Gabbar

Kareena Kapoor as Sunaina Singh, Aditya's late wife (cameo appearance)

Suman Talwar  as Digvijay Patil (Voice dubbed by Mayur Vyas)

Sunil Grover as Police driver Sadhuram

Jaideep Ahlawat as C.B.I Officer Kuldeep Pahwa

Sonu Sood as Balbir Singh

Raj Singh Arora as Govind Gawde

Manoj Chandila as Vikas Patil, Digvijay's son

Ninad Kamat as Police Inspector

Vikas Shrivastav as Inspector Vikas

Anuradha Chandan as Govind's mother

Jehangir R Karkaria as Comic Doctor of Patil Hospital

Narendra Jetly as Senior Doctor

Sai Ballal as Chandrakant Gawde

Jagdish Rajpurohit as Sub Inspector Yadav

Shivraj Wahekar as Police Commissioner Pant

Rajeev Kacho as Vishwas Sawant

Uttam Haldar as Ward boy

Krishna Raaz as Shanti Nagpal

Pradeep Shukla as Home Minister

Kumud Shaw as Dr Kumud

Jitendra Trehan as Dr Manoj Verma

Sai Bilal as Chandrakant Gakwe

Deepak Dhadwal as Principal, National College

Chitrangada Singh as an special appearance in an item song "Aao Raja"

Lagu-lagu Gabbar Is Back digubah oleh Chirantan Bhatt, Yo Yo Honey Singh dan Manj Musik, manakala liriknya ditulis oleh Manoj Yadav, Kumaar, Sahil Kaushal, Manj Musik, Raftaar dan Big Dhillon.