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The Reading Half Marathon is held on the streets of Reading in March of each year, with as many as 16,000 competitors from elite to fun runners.

Reading is the home of Reading Football Club, an association football club nicknamed The Royals, who were formed in 1871. Formerly based at Elm Park, the club plays at the 24,500 capacity Madejski Stadium in the south of the town near the M4 motorway. The stadium is named after chairman John Madejski, who has owned the club since 1991. Reading FC won promotion to the top flight for the first time in 2006 as Football League Championship champions with a national record of 106 points. They finished eighth in their first season as a top division club (just missing out on a UEFA Cup place) but were relegated the following season. The club's current manager is Brian McDermott.

Reading is a centre for rugby union football in the area, with the Guinness Premiership team London Irish as tenants at the Madejski Stadium. Reading is also home to another three senior semi-professional rugby clubs; Reading Abbey R.F.C., Redingensians R.F.C. and Reading R.F.C. The town hosts several other football variants, such as Gaelic football's St. Anthony's GAA, Australian rules football team Reading Kangaroos, and American football team Berkshire Renegades.

The sport of field hockey is represented by Reading Hockey Club, who play in the Men's Premier Division and the Women's Division One of English Hockey League. The Reading Rockets are a basketball club that play in the English Basketball League.

Like many Thames-side towns, Reading has rowing clubs, representing both town and university. The local Redgrave-Pinsent Rowing Lake provides training facilities, although much rowing is also conducted on the river itself. Dorney Lake, some 27 km (17 miles) to the east of Reading, provides a full international competition venue and will host the rowing events of the 2012 Summer Olympics. There are also several sailing lakes to the south and southwest of the town, the largest being Theale Lake (home of Burghfield Sailing Club) close to junction 12 of the M4. These lakes are also popular with water-skiing and jet-skiing enthusiasts.