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The song debuted on both the Billboard Hot 100 and Canadian Hot 100 at number 100. The next week the song rose on the Hot 100 from 100 to 15, with downloads for "Jai Ho" up nearly 500%, totaling about 103,000 for the soundtrack version and 27,000 for the Dolls' version, and also debuted on the Pop 100 chart at 20. Sales for both versions were figured into the song's chart placement. As of Monday 16 March, the song has entered top 40 on US radio.

On the Canadian Hot 100, the song reached its peak of number five on 14 March, 2009. "Jai Ho!" entered the UK Singles Chart at number 20 on March 22 and two weeks later, rose to  number three giving the Pussycat Dolls their sixth top three single in the country. The single debuted on the Australian ARIA Singles Chart at number ten and later peaked at number one, becoming the Dolls' second number one hit there.