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The mental and sexual health effects of biphobia on bisexual people are numerous. Studies show that bisexuals are often trapped in between the binaries of heterosexuality and homosexuality, creating a form of invalidation around their sexual identity. This often leads to recognized indicators of mental health issues such as low self-esteem and self-worth. These indicators and pressures to "choose" a sexual identity can, in many cases, lead to depression as they may feel they live in a culture that does not recognize their existence.

While doing research on sexual tendencies of women who have sex with women, one study, from the Journal of Bisexuality, concluded that bisexual women are more likely to engage in various high risk behaviors and were at a higher risk of contracting HIV and other sexually transmitted diseases. These behaviors have been attributed to the unlikeliness of bisexuals to discuss their sexuality and proper protection with health professionals for fear of judgement or discrimination, leaving them uneducated.