Jabarkan Muse – "Uprising" dari MTV Video Music Awards 2009

The All-American Rejects – "Gives You Hell"

Kid Cudi – "Remembering DJ AM"

Jay-Z & Alicia Keys – "Empire State of Mind"

Buzz Aldrin – presented Breakthrough Video

Sway Calloway – presented Best Video (That Should Have Won a Moonman)

Madonna – opened the show and paid tribute to Michael Jackson

Shakira and Taylor Lautner – presented Best Female Video

Jack Black and Leighton Meester – presented Best Rock Video

Miranda Cosgrove and Justin Bieber – introduced Taylor Swift

Pete Wentz and Gabe Saporta – introduced Lady Gaga

Nelly Furtado and Kristin Cavallari – presented Best Pop Video

Megan Fox and Adam Brody – introduced Green Day

Robert Pattinson, Kristen Stewart and Taylor Lautner – introduced an exclusive New Moon sneak peek preview

Chace Crawford and Ne-Yo – introduced Beyoncé

Diddy and Jamie-Lynn Sigler – presented Best Male Video

Gerard Butler and Alexa Chung – introduced Muse

Jennifer Lopez – presented Best Hip-Hop Video

Eminem and Tracy Morgan – presented Best New Artist

Serena Williams – introduced Pink

Jimmy Fallon and Andy Samberg – presented Video of the Year