Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.
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Question: This busy and popular hotel requires a Junior Chef. You will need to be energetic, enthusiastic with a minimum of five years' kitchen experience. You must assist the head and senior chefs in the creation of new dishes. Annual salaries range from 20,000 to 25,000 pounds. Please send or e-mail your CV to the Personnel Administrator Hydo Hotel, Mount Road Eastbourne BN207HZ eclaine.ward@hydrohotel.com. We are looking for a kitchen assistant willing to learn the day-to-day jobs in a professional kitchen environment. You must have a passion for cooking and basic understanding of spoken English. You will mainly be working for a well-known company and learn valuable cheffing skills. The salary will be based on experience but could start from 12,500 pounds to 14,000 pounds annually plus tips. Please forward your CV and a letter of recommendation. 01428644460 Kitchen Manager. Spirit Pub Company is looking for the best individuals to lead our kitchen teams through some very exciting new developments. As an experienced Kitchen Manager, you will play an important role in engaging the chef team by great leaderships to deliver excellent standards and guest satisfaction. We can offer a starting salary of up to 27,000 pounds plus a bonus worth an extra 2,000 pounds per year for the right candidates. If you have what it takes to join our company, please provide your CV with your application. 07044338313 Food Service Assistant, South East Food Service Assistant required. Salary starts from 15,000 pound to 20,000 pounds annually. We are seeking a General Assistant for a Care Home East Sussex. Assisting the Cook and kitchen team in the food preparation of freshly prepared meals, washing up and cleaning of all kitchen areas. Previous experience would be an advantage. 07044338228

Answer: Which job probably offers the second highest salary?


Question: The 80-string koto (or hachijugen) was an invention of Japanese composer Michio Miyagi which appeared In 1923.

Answer: By whom was 80-string koto discovered?


Question: Polyglutamine diseases, including Huntington's disease, designate a group of nine neurodegenerative disorders characterized by the presence of a toxic polyglutamine expansion in specific target proteins. Using cell and mouse models, we have shown that expanded polyglutamine led to activation of the stress kinase JNK and the transcription factor AP-1, which are implicated in neuronal death. Polyglutamine expansion-induced stress shared common features with protein-damaging stress such as heat shock, because activation of JNK involved inhibition of JNK phosphatase activities. Indeed, expanded polyglutamine impaired the solubility of the dual-specificity JNK phosphatase M3/6. Aggregation of M3/6 by polyglutamine expansion appeared to be indirect, because M3/6 was not recruited into polyglutamine inclusions. The heat shock protein HSP70, which is known to inhibit JNK during the heat shock response, suppressed polyglutamine-mediated aggregation of M3/6 and activation of JNK. Interestingly, levels of HSP70 were down-regulated by polyglutamine expansion. We suggest that reduction of HSP70 by expanded polyglutamine is implicated in aggregation and inhibition of M3/6 and in activation of JNK and AP-1.

Answer:
Which protein is affected by dusp8 activation?