Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

[EX Q]: Ataxin-3 (AT3) is the protein that triggers the inherited neurodegenerative disorder spinocerebellar ataxia type 3 when its polyglutamine (polyQ) stretch close to the C-terminus exceeds a critical length. AT3 consists of the N-terminal globular Josephin domain (JD) and the C-terminal disordered one. It cleaves isopeptide bonds between ubiquitin monomers, an event involved in protein quality control mechanisms. AT3 has been implicated in the pathway that sorts aggregated protein to aggresomes via microtubules, in which dynein and histone deacetylase 6 (HDAC6) also seem to be involved. By taking advantage of small angle X-ray scattering (SAXS) and surface plasmon resonance (SPR), we have investigated the interaction of AT3 with tubulin and HDAC6. Based on SAXS results, the AT3 oligomer, consisting of 6-7 subunits, tightly binds to the tubulin hexameric oligomer in a "parallel" fashion. By SPR analysis we have demonstrated that AT3 binds to tubulin dimer with a 50nM affinity. Binding fits with a Langmuir 1:1 model and involves a single binding interface. Nevertheless, the interaction surface consists of three distinct, discontinuous tubulin-binding regions (TBR), one located in the JD, and the two others in the disordered domain, upstream and downstream of the polyQ stretch. In the absence of any of the three TBRs, the affinity is drastically reduced. By SPR we have also provided the first evidence of direct binding of AT3 to HDAC6, with affinity in the range 0.1-1M. These results shed light on the interactions among the components of the transport machinery that sorts aggregate protein to the aggresome, and pave the way to in vivo studies aimed at further clarifying their roles.
[EX A]: Which is the protein implicated in Spinocerebellar ataxia type 3?

[EX Q]: The War of the Spanish Succession was a European conflict of the early 18th century, triggered by the death of the childless Charles II of Spain in November 1700. His closest heirs were members of the Austrian Habsburg and French Bourbon families; acquisition of an undivided Spanish Empire or Monarchy by either threatened the European balance of power. Charles left his throne to Louis XIV's grandson Philip who was proclaimed King of Spain on 16 November 1700. Disputes over the separation of the Spanish and French crowns, division of territories and commercial rights led to war in 1701 between the Bourbons of France and Spain and the Grand Alliance, whose candidate was Archduke Charles, younger son of Habsburg Emperor Leopold. By 1710, fighting was deadlocked; Allied victories in Italy and the Low Countries had driven the French back to their borders but they could not achieve a decisive breakthrough while Philip was secure in Spain. When Archduke Charles succeeded his brother Joseph I as Emperor in 1711, Britain effectively withdrew, forcing its Allies to make peace and leading to the 1713 Treaty of Utrecht, followed in 1714 with Rastatt and Baden. Philip was confirmed as King of Spain and renounced the French throne; Spain retained the bulk of its pre-war territories outside Europe with their European territories divided between Austria, Britain and Savoy. Longer term impacts included Britain's emergence as the leading maritime and commercial power, the beginning of the decline of the Dutch Republic, the creation of a centralised Spanish state and the acceleration of the break-up of the Holy Roman Empire.
[EX A]: Who was Emperor first, Archduke Charles or his brother Joseph I?

[EX Q]: Every immigrant leads a double life. Every immigrant has a double identity and a double vision, being suspended between an old and a new home, an old and new self. The very notion of a new home, of course, is in a sense as impossible as the notion of new parents. Parents are who they are; home is what it is. Yet home, like parentage, must be legitimized through love; otherwise, it is only a fact of geography or biology. Most immigrants to America found their love of their old homes betrayed: they did not really abandon their countries; their countries abandoned them. In America, they found the possibility of a new love, the chance to nurture new selves. Not uniformly, not without exceptions. Every generation has its Know-Nothing movement. Its understandable fear and hatred of alien invasion is as true today as it always was, but in spite of all this, the American attitude remains unique. Throughout history, exile has been a disaster; America turned it into a triumph and placed its immigrants in the center of a national epic. The epic is possible because America is an idea as much as it is a country. America has nothing to do with loyalty to a dynasty and very little to do with loyalty to particular place, but everything to do with loyalty to a set of principles. To immigrants, those principles are especially real because so often they were absent or violated in their native lands. It was no accident in the '60 and '70s, when alienation was in flower, that it often seemed to be "native" Americans who felt alienated, while aliens or the children of aliens upheld the native values. The immigrant's double vision results in a special, somewhat skewed perspective on America that can mislead but that can also find revelation in the things that to native Americans are obvious. Psychiatrist Robert Coles speaks of those "who straddle worlds and make of that very experience a new world." "Home is where you are happy." Sentimental, perhaps, and certainly not conventionally patriotic, but is appropriate for a country that wrote the pursuit of happiness into its founding document. That continues for the immigrant in America, and it never stops, but it comes to rest at a certain moment. The moment is hard to pin down, but it occurs perhaps when the immigrant's double life and double vision joint together toward a single state of mind. When the old life, the old home fade into a certain unreality: places one merely visits, in fact or in the mind, practicing the tourism of memory. It occurs when the immigrant learns his ultimate lesson: above all countries, America, if loved, returns love.
[EX A]:
What can be the best title of the passage?