In this task, you are given a multiple-choice question about healthcare. Answer the question based on your information and classify your answers into '1', '2', '3', and '4'.

Question: Regarding gastroesophageal reflux disease, which of the following statements is true? 
 Options:  <1> The intensity and frequency of heartburn has little relationship with the presence and severity of endoscopic esophagitis. <2> Most patients with gastroesophageal reflux disease have esophagitis at endoscopy. <3> Treatment with proton pump inhibitors usually achieves good control of symptoms but is not superior to placebo in the endoscopic cure of esophagitis. <4> Barrett's esophagus can progress to low-grade epithelial dysplasia, high-grade epithelial dysplasia, and finally squamous cell carcinoma of the esophagus. <5> Endoscopic screening and surveillance programs have unequivocally shown to be able to reduce mortality in patients with Barrett's esophagus.
1

Question: They incorporate sterols to their plasma membrane: 
 Options:  <1> Mycobacterium tuberculosis. <2> Micrococcus luteus. <3> Mycoplasma pneumoniae <4> Chlamydia trachomatis. <5> Rickettsia prowazekii.
3

Question: In the presence of a patient with Crohn's disease who is going to start treatment with biological anti-TNF therapy (antibodies against tumor necrosis factor alpha), which of the following diagnostic tests is not necessary before initiating such therapy? 
 Options:  <1> Evaluation of the immune stage by lymphocyte count. <2> Serology of hepatitis B virus (HBV). <3> Tuberculin test. <4> Chest x-ray. <5> Serology of the human immunodeficiency virus (HIV).

1