In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".

Q: Paragraph: Porcine epidemic diarrhea virus (PEDV) is a porcine enteropathogenic coronavirus that has received increasing attention since the emergence of a PEDV variant worldwide. Previous studies have shown that PEDV ORF3 encodes an ion channel protein. However, its influence on cell cycle and subcellular structure still require more research. In this study, we developed a Vero cell line that stably expresses PEDV ORF3 gene. Subcellular localization and influences of PEDV ORF3 on host cells were investigated. We further verified whether or not this gene enhances virus production. The results showed that PEDV ORF3 protein localizes in the cytoplasm and affects cell cycle progression by prolonging the S phase. In addition, the ORF3-expressing Vero cells had more vesicles than the host Vero cells. Furthermore, the attenuated PEDV rather than virulent PEDV could grow better in ORF3-expressing Vero cells. The expression level of the PEDV nucleocapsid protein also increased. These results provided information on the function of PEDV ORF3 and were helpful in understanding the mechanisms of PEDV replication. 
 Title: Porcine epidemic diarrhea virus ORF3 gene prolongs S-phase, facilitates formation of vesicles and promotes the proliferation of attenuated PEDV

A: True
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Q: Paragraph: The replicase of mouse hepatitis virus strain JHM (MHV-JHM) is encoded by two overlapping open reading frames, ORF1a and ORF1b, which are translated to produce a 750-kDa precursor polyprotein. The polyprotein is proposed to be processed by viral proteinases to generate the functional replicase complex. To date, only the MHV-JHM amino-terminal proteins p28 and p72, which is processed to p65, have been identified. To further elucidate the biogenesis of the MHV-JHM replicase, we cloned and expressed five regions of ORF1a in bacteria and prepared rabbit antisera to each region. Using the immune sera to immunoprecipitate radiolabeled proteins from MHV-JHM infected cells, we determined that the MHV-JHM ORF1a is initially processed to generate p28, p72, p250, and p150. Pulse±chase analysis revealed that these intermediates are further processed to generate p65, p210, p40, p27, the MHV 3C-like proteinase, and p15. A putative replicase complex consisting of p250, p210, p40, p150, and a large protein (Ͼ300 kDa) coprecipitate from infected cells disrupted with NP-40, indicating that these proteins are closely associated even after initial proteolytic processing. Immunofluorescence studies revealed punctate labeling of ORF1a proteins in the perinuclear region of infected cells, consistent with a membrane-association of the replicase complex. Furthermore, in vitro transcription/translation studies of the MHV-JHM 3Cpro and flanking hydrophobic domains confirm that 3C protease activity is significantly enhanced in the presence of canine microsomal membranes. Overall, our results demonstrate that the MHV-JHM ORF1a polyprotein: (1) is processed into more than 10 protein intermediates and products, (2) requires membranes for efficient biogenesis, and (3) is detected in discrete membranous regions in the cytoplasm of infected cells. 
 Title: Processing of the Coronavirus MHV-JHM Polymerase Polyprotein: Identification of Precursors and Proteolytic Products Spanning 400 Kilodaltons of ORF1a

A: False
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Q: Paragraph: C ell-to-cell spread of retroviruses via virological synapse (VS) contributes to overall progression of disease. VS are specialized pathogen-induced cellular structures that facilitate cell-to-cell transfer of HIV-1 and HTLV-1. VS provide a mechanistic explanation for cell-associated retroviral replication. While VS share some common features with neurological or immunological synapses, they also exhibit important differences. The role of VS might not be limited to human retroviruses and the emerging role of a plant synapse suggests that VS might well be conserved structures for cell-cell spreading of both animal and plant viruses. Dissection of the VS is just at its beginning, but already offers ample information and fascinating insights into mechanisms of viral replication and cell-to-cell communication. The complex functioning of biological systems requires the capacity of cells to interact in a synchronized manner. The capacity of cells to come in close contact with one another enables rapid exchange of information through directed secretion. In complex systems such as the nervous and immune systems, characteristic rearrangements of plasma membrane proteins appear at the cell-cell junction, called synapse. A synapse is defined as "a stable adhesive junction across which information is relayed by directed secretion".^ The concept of the neural synapse (NS) was first introduced over a century ago and was depicted as a stable structure organized and specialized in intercellular signaling between neurons. Plasma membranes of the pre and post-synaptic neurons are contiguous and information is conveyed to the downstream cell via secretion of neurotransmitters. In order to generate a favorable microenvironment, stabilization of synapse by scaffolding proteins, mainly cadherins and other adhesion molecules, is required (reviewed in re£ 1). In the immune system, interactions between T cells and antigen presenting cells (APC) are essential for an effective adaptive immune response. By analogy with the nervous system, these specialized interactions occur via an immunological synapse (IS). The concept of the IS has been extended to several types of cell-cell interactions within the context of the immune system (signaling via receptor engagement, lytic granules, directed secretion of cytokines) since its first description 20 years ago (reviewed in refs. 2,3) . Although the IS shares many similarities with the NS, it also differs in two aspects. First, the panel of receptors and adhesion proteins recruited to the IS diverges from those in the neural synapse: integrins play the central role in stabilizing IS. Second, the establishment of an IS is a dynamic process between moving cells, whereas the neural synapse is long-lived. Therefore, in 
 Title: Virological Synapse for Cell-Cell Spread of Viruses

A:
False
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