Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

[Q]: Zach Conroy and Celeste Mercier are the hottest couple on and off the ice until a nasty spill knocks Celeste out of competition. With their dreams of gold medals put in ice and their romance chilling as a result, Zach has to find another partner for the championship in Paris. And fast. In glides Alejandra Alex Delgado, a beautiful, tough talking hockey player with fierce moves and a fearless skating style. Zachs willing to give her a chance, but his coach, Bryan quits in a huff to train Zachs biggest rivals, Cindy Halgyord and Jason Bright. That leaves Zach with a newbie partner and no coach until he convinces former figure skating champ Jackie Dorsey to step in. She puts Zach and Alex through their paces, but the hardest trick of all is keeping them focused on practice and not each other as they bicker and struggle with a growing attraction both on and off the ice. Jackie knows these kids are fire and ice, and sees the chemistry between them. She also knows that if they cant get it together, theyll set the skating world on fire. That is, if the heat between them doesnt cause a total meltdown. With the competition fierce and the stakes higher than ever, the only way Zach and Alex can win is by putting aside their feelings and pulling out a secret weapon: the deadly Pamchecko jump. But is it worth the risk? Can Zach rise above the past and his history of injuring partners? And can Alex prove that shes got the moves to match her passion? It all comes down to the biggest moment of their lives when they learn that sometimes on the ice and in life youve got to risk it all.
[A]: Who refuses to coach the pair?


[Q]: The blockage of transcription elongation by RNA polymerase II (pol II) at a DNA damage site on the transcribed strand triggers a transcription-coupled DNA repair (TCR), which rapidly removes DNA damage on the transcribed strand of the expressed gene and allows the resumption of transcription. To analyze the effect of UV-induced DNA damage on transcription elongation, an in vitro transcription elongation system using pol II and oligo(dC)-tailed templates containing a cyclobutane pyrimidine dimer (CPD) or 6-4 photoproduct (6-4PP) at a specific site was employed. The results showed that pol II incorporated nucleotides opposite the CPD and 6-4PP and then stalled. Pol II formed a stable ternary complex consisting of pol II, the DNA damage template, and the nascent transcript. Furthermore, atomic force microscopy imaging revealed that pol II stalled at the damaged region. These findings may provide the basis for analysis of the initiation step of TCR.
[A]: Which gene strand is targeted by transcription-coupled repair (TCR)?


[Q]: Autosomal-recessive hereditary spastic paraplegias (AR-HSP) consist of a genetically diverse group of neurodegenerative diseases characterised by pyramidal tracts dysfunction. The causative genes for many types of AR-HSP remain elusive. We tried to identify the gene mutation for AR-HSP with cerebellar ataxia and neuropathy. This study included two patients in a Japanese family with their parents who are first cousins. Neurological examination and gene analysis were conducted in the two patients and two normal family members. We undertook genome-wide linkage analysis employing single nucleotide polymorphism arrays using the two patients' DNAs and exome sequencing using one patient's sample. We detected a homozygous missense mutation (c.4189T>G, p.F1397V) in the lysosomal trafficking regulator (LYST) gene, which is described as the causative gene for Chediak-Higashi syndrome (CHS). CHS is a rare autosomal-recessive syndrome characterised by hypopigmentation, severe immune deficiency, a bleeding tendency and progressive neurological dysfunction. This mutation was co-segregated with the disease in the family and was located at well-conserved amino acid. This LYST mutation was not found in 200 Japanese control DNAs. Microscopic observation of peripheral blood in the two patients disclosed large peroxidase-positive granules in both patients' granulocytes, although they had no symptoms of immune deficiency or bleeding tendency. We diagnosed these patients as having adult CHS presenting spastic paraplegia with cerebellar ataxia and neuropathy. The clinical spectrum of CHS is broader than previously recognised. Adult CHS must be considered in the differential diagnosis of AR-HSP.
[A]:
Which mutated gene causes the Chédiak–Higashi Syndrome?