Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

Example Input: The spin-off movie focuses on Matt Stifler (Tad Hilgenbrinck), the younger brother of Steve Stifler from the previous American Pie films. Matt is eager to enter the family business of making porno films, so he can prove to his older brother that he is "up to the standards of the Stifmeister." Stifler and his friends play a prank on the band students during the seniors' graduation ceremony by spraying pepper spray on the mouthpieces of the band's instruments. Matt narrowly escapes getting caught and stows the can of pepper spray in his front pocket, which subsequently leaks onto his genitals. The band members begin to play "Pomp and Circumstance", only to become too irritated by the pepper spray to continue. During the commotion, one of the students trips and pulls down the back curtain, which reveals Matt, who is washing his pepper-spray-soaked genitals in a drinking fountain, in front of the whole school.The band leader Elyse Houston (Arielle Kebbel) demands that Stifler be punished for ruining their performance, stating that he always gets away with his pranks because he is the school's star football player. The school's guidance counselor Chuck Sherman ("The Sherminator") (played by Chris Owen from the original American Pie films) decides that a worthwhile punishment would be for Stifler to attend band camp, in the hopes that he would make friends with the "bandies" and not follow in the footsteps of his much-hated brother.When first arriving, Matt is very disrespectful to the rules and also everyone at Tall Oaks and gets his band group in trouble. He is sent to the office of Noah Levenstein (Jim's father) (Eugene Levy), who works as the camp MACRO (Morale and Conflict Resolution Officer; he's filling in for Michelle as she is now pregnant), recommends that he try to fit in and earn the band's trust, which is the last thing that Matt wants to do. However, a phone call from a friend, who says that earning their trust will get him better footage for his video, inspires Matt to be much more respectful and humble towards the band.Matt finds out that his dorky roommate Ernie Kapolwitz (Jason Earles) has an extensive knowledge of spy cameras and technology, so Matt agrees to let him help with the filming, making his first friend at Tall Oaks. As Matt "is earning the band's trust" he gets into a big confrontation with their rival band leader, Brandon Van der Camp (Matt Barr), and even goes as far as to accept a duel challenge made by him, having no idea what a "duel" is.It turns out that a duel is the two performers showing off their music skills, with Brandon playing the snare drum, and Matt playing the triangle. Throughout the majority of the competition it seems that Brandon is the heavy favourite; during Brandon's second round of performing, Matt walks away humiliated. As Brandon is about to be declared the official winner, Matt comes back playing the bagpipes to the tune of 'Play That Funky Music' showing that he is actually a decent player, surprising everyone, including Elyse, who is pleasantly surprised by Matt's performance.As Matt is still recording the various happenings at the camp, he gradually becomes friends with some of the band members from his school. He gains some respect from Ernie after Matt sets him up on a date with tattooed bandmate Chloe (Crystle Lightning). Matt starts falling for Elyse, who is revealed to have been a friend of Matt's in 8th grade but had a falling out after Matt started hanging around with Steve and his friends and acting like The Stifmeister. Matt is eventually caught with his movies, and his once-friends want nothing more to do with him.After another talk with Jim's Dad, Matt comes to realize that his brother is not as great or cool as he once thought and that following in his footsteps is not a good idea, so he deletes all the videos he recorded at band camp. He even enlists the help of the band to help Elyse get the band scholarship that Stifler
Example Output: Who is the younger brother of Steve Stifler?

Example Input: It is increasingly clear that the normal protein alpha-synuclein is in some manner closely associated with presynaptic components of select neuronal types within the adult human central nervous system (CNS) and, in addition, that in its pathologically altered state alpha-synuclein aggregates selectively in the form of filamentous inclusion bodies during certain progressive neurodegenerative disorders, such as familial and sporadic Parkinson's disease. By having the antibody AFshp raised specifically to alpha-synuclein to label Parkinson disease-specific Lewy bodies and Lewy neurites as well as synaptic boutons containing the unaltered protein, an initial attempt is made to map the overall distribution pattern and describe the staining behavior of the immunoreactive punctae in select regions of the prosencephalon. Neocortical immunolabeling is most prominent in the prodigious, but incompletely myelinated, association fields and faintest in the heavily myelinated primary motor and primary sensory fields, with the premotor and first order sensory association areas occupying an intermediate position. Of the thalamic grays evaluated, those containing powerfully myelinated fiber tracts (e.g. centrum medianum, habenular complex) show the weakest immunolabeling, whereas, less sturdily myelinated structures are highly immunoreactive. The fact that the immunostaining spectrum for normal alpha-synuclein is so broad, together with the fact that some thalamic sites actually are immunonegative leads to the following conclusions (1) alpha-synuclein, although present in the synaptic boutons of many nerve cells in the adult human CNS, is by no means ubiquitous there, and (2) neuronal types lacking the normal protein cannot generate the Parkinson's disease-specific filamentous pathology.
Example Output: Against which protein is the antibody used for immonostaining of Lewy bodies raised?

Example Input: Zandalee Martin (Erika Anderson) is a young boutique store owner living in New Orleans who is sexually frustrated and feeling unfulfilled with her marriage to Thierry Martin (Judge Reinhold), and eventually gets tangled in a passionate, sensual and torrid adulterous affair with her husband's mysterious and free spirited old friend Johnny Collins (Nicholas Cage).Zandalee and Thierry's marriage has hit a snag and seems to be eroding due to his lack of passion. Zan needs to explore, while Thierry wants to withdraw, and has become more and more distant and impotent in their relationship. He used to be a poet, but now has taken over the familys communications business after the death of his father. As time goes on, Thierry has to sell the business and become basically a (vice president) figurehead. He is emotionally adrift as his dreams give way to disillusionment.Johnny, an artist painter by trade, has been working for Thierry's business to help support his paintings. His only religion is self-gratification. Johnny also sells and mules cocaine for a local drug dealer as another source of income for himself.Having not seen each other in a while, the two run in to each other at a bachelor's party. After the party, Thierry brings Johnny home to meet Zandalee and his grandmother Tatta (Viveca Lindfors). While talking about old times, Johnny offers to paint a portrait of Thierry at their home.Later, after finishing the painting, Johnny shows it to Thierry, Zandalee and Tatta. While they go off to other rooms and sensing Zandalee's frustration and vulnerability, Collins makes a pass at her. Johnny continues to pursue her and when they run into each other during a rain storm, he takes advantage and moves in by seducing her, first in his loft in an angry passionate scene, (interestingly, Zan's wedding ring is on the table next to the bed) which is followed by him erotically finger painting her. Their sexual liaisons continue to occur in various places including her laundry room a top a washing machine while Thierry and guests are having dinner. Thierry soon suspects the two are having an affair.As the affair intensifies, Johnny meets Zandalee in a local church and asks her to leave her husband. However, Zandalee feels that she must never abandon her true love Thierry, and quickly ends her affair with Johnny after he forces himself on her in the confessional. She and Thierry re-commit themselves to each other, but Johnny, now obsessed with her, will not be brushed off that easily. He tracks them to their vacation spot in the Bayou. All of this puts the three on a destructive collision course with a tragic sequence of events.When Thierry figures out that Johnny has indeed been having an affair with Zandalee, he becomes drunk and confrontational (he pulls a gun out) leading to him becoming reckless when he takes Zan and Johnny for a speedboat ride on the Bayou, which ends when he falls off the boat and drowns, refusing to be saved by either Zan or Johnny who dive into the water to save him.Both Zandalee and Johnny become distraught by Thierry's death and begin to isolate themselves with Zan jogging for long periods and Johnny trying to work on his paintings, but becoming more self-destructive. In one scene, Johnny, in a rage rips up some of his paintings and pours black paint all over himself. He also consumes some of the cocaine he is supposed to sell, which gets him in trouble with his supplier.When Johnny meets with Zandalee with the hopes to re-start their romance and have a possible future together now that Thierry is out of the picture, she remains emotionally distant and instead goes for a walk along the Old Quarter with Johnny following her. But in the final scene, when Johnny's drug supplier attempts to kill him in a drive-by shooting outside the church that Zandalee frequents, she sees what is about to happen and shields Johnny, getting fatally shot in the process. The drug dealer flees from the scene of the crime (saying "you've got to make accounts payable man"), leaving behind Johnny, now alone, as he
Example Output:
who is zandalee's husband?