In this task, you are given a sentence from the research paper and your task is to classify the given sentence into the following categories: Background (Why is this problem important? What relevant works have been created before? What is still missing in the previous works? What are the high-level research questions? How might this help other research or researchers?), Purpose (What specific things do the researchers want to do? What specific knowledge do the researchers want to gain? What specific hypothesis do the researchers want to test?), Method (How did the researchers do the work or find what they sought? What are the procedures and steps of the research?), or Finding (What did the researchers find out? Did the proposed methods work? Did the thing behave as the researchers expected?). 

Aminoacylated tRNAs were purified by reversed-phase HPLC on an RP-18 HPLC column ( 250 mm × 10.5 mm , Merck ) equilibrated with buffer ( 20 mM ammonium acetate , pH 5.0 , 10 mM magnesium acetate , 400 mM NaCl ) using a gradient of 0 % -15 % ) ethanol.
method

even at 72 h after treatment ( P < 0.05 ).
finding

This study focuses on the visualization of smoke plume discharge and an uncertainty analysis is not applicable.

purpose