Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

[Q]: Vukadin Vukadinovic (born 14 December 1990) is a professional Serbian football midfielder currently playing for FC Fastav Zlin on loan from FK Baumit Jablonec.
[A]: For which sports team does Vukadin Vukadinović play?


[Q]: It was 1:30 a.m. Monday at London's Savoy Hotel and, with autograph books and cameras, a group of brighteyed tennis fans of all ages were waiting for just a glimpse of Maria Sharapova . The 17-year-old girl defeated champion Serena Williams 6-1 6-4 in the Wimbledon final on Saturday in London to become the first Russian to win a single title at the championships. A silver lining to a depressing, rain-hit Wimbledon, she has raised heated passion. Nobody was complaining, though, for the 1.83-metre Sharapova has created a welcome wave of interest in tennis, something missing in recent years. Not even Anna Kournikova evoked such passion when she broke on to the scene. To look good on court is one thing, but Sharapova can actually play the game too. Extremely well. She proved it over 13 days at the world's most prestigious tournament. Playing tennis of the highest quality throughout, the Russian's triumph not only lifted the tournament from fits of rain-induced depression but also salvaged(;) the season for the woman's tour. Sharapova was given no easy ride in the tournament, having to beat 1999 champion Lindsay Davenport in the semifinals and twice-champion Serena Williams for the title. Neither caused her much concern. "I don't remember too much about the final," Sharapova giggled. "I was in my own place." She will find it increasingly hard to find any place to call her own from now on after advertisers identified her as the most marketable woman in sport. In Russia she is sensational news. She headlined the news bulletins and was telephoned by former Russian President Boris Yeltsin after her triumph. Sharapova will return to her adoptive Florida home knowing life will never be the same again. But the girl who arrived in the US aged seven with her father and just US$700 is determined not to let fame and riches detract from her number one love, tennis. "I know things will start coming up and that many more things will want to get involved, but I want to keep my head cool and play tennis," she said.
[A]: Who has won the most times in Wimbledon?


[Q]: In eukaryotic cells, there are two sub-pathways of nucleotide excision repair (NER), the global genome (gg) NER and the transcription-coupled repair (TCR). TCR can preferentially remove the bulky DNA lesions located at the transcribed strand of a transcriptional active gene more rapidly than those at the untranscribed strand or overall genomic DNA. This strand-specific repair in a suitable restriction fragment is usually determined by alkaline gel electrophoresis followed by Southern blotting transfer and hybridization with an indirect end-labeled single-stranded probe. Here we describe a new method of TCR assay based on strand-specific-PCR (SS-PCR). Using this method, we have investigated the role of DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase-related protein kinases (PIKK) family, in the TCR pathway of UV-induced DNA damage. Although depletion of DNA-PKcs sensitized HeLa cells to UV radiation, it did not affect the ggNER efficiency of UV-induced cyclobutane pyrimidine dimers (CPD) damage. We postulated that DNA-PKcs may involve in the TCR process. To test this hypothesis, we have firstly developed a novel method of TCR assay based on the strand-specific PCR technology with a set of smart primers, which allows the strand-specific amplification of a restricted gene fragment of UV radiation-damaged genomic DNA in mammalian cells. Using this new method, we confirmed that siRNA-mediated downregulation of Cockayne syndrome B resulted in a deficiency of TCR of the UV-damaged dihydrofolate reductase (DHFR) gene. In addition, DMSO-induced silencing of the c-myc gene led to a decreased TCR efficiency of UV radiation-damaged c-myc gene in HL60 cells. On the basis of the above methodology verification, we found that the depletion of DNA-PKcs mediated by siRNA significantly decreased the TCR capacity of repairing the UV-induced CPDs damage in DHFR gene in HeLa cells, indicating that DNA-PKcs may also be involved in the TCR pathway of DNA damage repair. By means of immunoprecipitation and MALDI-TOF-Mass spectrometric analysis, we have revealed the interaction of DNA-PKcs and cyclin T2, which is a subunit of the human transcription elongation factor (P-TEFb). While the P-TEFb complex can phosphorylate the serine 2 of the carboxyl-terminal domain (CTD) of RNA polymerase II and promote transcription elongation. A new method of TCR assay was developed based the strand-specific-PCR (SS-PCR). Our data suggest that DNA-PKcs plays a role in the TCR pathway of UV-damaged DNA. One possible mechanistic hypothesis is that DNA-PKcs may function through associating with CyclinT2/CDK9 (P-TEFb) to modulate the activity of RNA Pol II, which has already been identified as a key molecule recognizing and initializing TCR.
[A]:
Which gene strand is targeted by transcription-coupled repair (TCR)?