Given a passage, construct a question on the basis of the information present in the passage. Construct the question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) it answer uses text phrases from the passage. Avoid creating questions that (i) can't be answered correctly without actually understanding the passage and (ii) uses the same words or phrases given in the passage.

Input: Consider Input: To establish a single-tube single-run multiplex PCR technique that can detect single or mixed samples with four species of Plasmodium.', 'Folding primers were designed based on the fast nested PCR. The reaction component concentrations were optimized and the primers were selected based on the annealing temperature. The established single-tube single-run folding-primer multiplex PCR (FP-PCR) was tested for its sensitivity and specificity to detect single-species and mixed samples with P. vivax, P. falciparum, P. ovale (including P. ovale wallikeri) and/or P. malariae.', 'In all the seven experimental repeats, FP-PCR successfully detected single-species infection for all the four species, with the detection limit reaching or close to 1 parasite/µl blood. For mixed infections with 2-4 species at different densities with the highest being 100 times of the lowest, FP-PCR identified all the species in each combination in 57 out of 84 tests. Further, in 10 dried blood samples on filter paper from healthy subjects, no FP-PCR amplification was found, except weak formation of dimers.

Output: Does [ Single-tube Single-run Multiplex PCR detect Mixed Samples with Four Species of Plasmodium ]?


Input: Consider Input: Thermal microdebridement for the treatment of chronic tendinopathy has recently been introduced. The effect of thermal microdebridement on the biomechanical properties of human tendons, however, remains unknown.', 'Thermal microdebridement does not affect the biomechanical properties of human patellar tendons in a cadaveric model at the time of initial treatment.', 'Controlled laboratory study.', 'The central 15 mm of 12 matched, human (mean age, 71 years; 8 male, 4 female), fresh-frozen patellar tendons was divided into 3 equal 5-mm specimens. The treatment group (n = 12) underwent thermal microdebridement with a radiofrequency probe. A sham treatment group (n = 12) underwent insertion of a deactivated probe. The control group (n = 12) underwent no treatment. After treatment, each specimen was tested to failure in a servo-hydraulic materials testing machine at an elongation rate of 3 mm/s. One-way repeated measures analysis of variance was used to determine differences between groups.', 'No significant difference in ultimate stress at failure, elastic modulus, strain energy density, or strain at maximum load was found between the groups. The ultimate stress at failure for the treatment, sham, and control groups was 61.0, 66.7, and 63.0 MPa, respectively (P = .653), and the strain at maximum load was 0.12, 0.11, and 0.09, respectively (P = .279).

Output: Does thermal microdebridement affect the time zero biomechanical properties of human patellar tendons?


Input: Consider Input: Greater glycemic variability and lack of predictability are important issues for patients with type 1 diabetes. Dietary factors are one of the contributors to this variability, but how closely diet is linked to glycemic fluctuation on a daily basis has not been investigated. We examined the association between carbohydrate intake and glycemic excursion in outpatients.', 'A total of 33 patients with type 1 diabetes were included in the analyses (age 44.5\xa0±\xa014.7\xa0years, diabetes duration 15.1\xa0±\xa08.3\xa0years, 64% female, 30% using insulin pump, glycated hemoglobin 8.1\xa0±\xa01.3%). Time spent in euglycemia (70-180\xa0mg/dL), hyperglycemia (>180\xa0mg/dL) and hypoglycemia (<70\xa0mg/dL) of consecutive 48-h periods of continuous glucose monitoring data were collected together with simultaneous records of dietary intake, insulin dose and physical activity. Correlation analyses and multiple regression analyses were used to evaluate the contribution of carbohydrate intake to time spent in the target glycemic range.', 'In multiple regression analyses, carbohydrate intake (β\xa0=\xa00.53, P\xa0=\xa00.001), basal insulin dose per kg per day (β\xa0=\xa0-0.31, P\xa0=\xa00.034) and diabetes duration (β\xa0=\xa00.30, P\xa0=\xa00.042) were independent predictors of time spent in euglycemia. Carbohydrate intake (β\xa0=\xa0-0.51, P\xa0=\xa00.001) and insulin pump use (β\xa0=\xa0-0.34, P\xa0=\xa00.024) were independent predictors of time spent in hyperglycemia. Insulin pump use (β\xa0=\xa00.52, P\xa0<\xa00.001) and bolus insulin dose per kg per day (β\xa0=\xa00.46, P\xa0=\xa00.001) were independent predictors of time spent in hypoglycemia.


Output: Is carbohydrate intake associated with time spent in the euglycemic range in patients with type 1 diabetes?