Given a passage with a context and an answer, construct a question on the basis of the information present in the passage. Construct the question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) it answer uses text phrases from the passage. Avoid creating questions that (i) can't be answered correctly without actually understanding the passage and (ii) uses the same words or phrases given in the passage.
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Question: Context: Vessel formation is a crucial event in tissue repair after injury. Thus, one assumption of innovative therapeutic approaches is the understanding of its molecular mechanisms. Notwithstanding our knowledge of the role of Protein Kinase C epsilon (PKCε) in cardio-protection and vascular restenosis, its role in vessel progenitor differentiation remains elusive.', 'Given the availability of PKCε pharmacological modulators already tested in clinical trials, the specific aim of this study is to unravel the role of PKCε in vessel progenitor differentiation, with implications in vascular pathology and vasculogenesis.', 'Mouse Peri-Vascular Adipose Tissue (PVAT) was used as source of mesenchymal vessel progenitors. VEGF-induced differentiation of PVAT cells down-regulates both PKCε and p-PAK1 protein expression levels. PKCε overexpression and activation: i) reduced the expression levels of SMA and PECAM in endothelial differentiation of PVAT cells; ii) completely abrogated tubules formation in collagen gel assays; iii) increased the expression of p-PAK1.
Answer: PKCε negatively interferes with vessel progenitor differentiation via interaction with PAK-1.

Answer: Is pKCε a negative regulator of PVAT-derived vessel formation?


Question: Context: To explore the role of SCF/c-Kit signaling in the invasion of bladder cancer T24 cells.', 'Western blotting was used to detect the expression of c-Kit and PI3K pathway activation stimulated by stem cell factor (SCF) in T24 cells. The invasiveness of T24 cells before and after SCF stimulation and Wortmannin (aspecific PI3K inhibitor) treatment was evaluated using Transwell invasion assay (direct and indirect counting methods).', 'T24 cells expressed c-Kit protein and showed obvious Akt phosphorylation after stimulation with SCF (1 ng/ml) for 24 h. Compared to the control group, SCF stimulation (1 ng/ml) caused a greater number of T24 cells to migrate through the polycarbonate film (P<0.01), and this effect was blocked by the application of Wortmannin before the stimulation.
Answer: SCF/c-Kit signaling promotes the invasiveness of T24 cells, and this effect is mediated by the PI3K pathway.

Answer: Does [ SCF/c-Kit signaling promote invasion of T24 cells via PI3K pathway ]?


Question: Context: The role of genistein in the effect of growth factors (KGF and EGF) on the growth of HPV 16 DNA-immortalized human cervical epithelial cells (HCE 16/3 cells) was studied.', 'Genistein affected the modulation effect of the growth factors on the growth of HCE 16/3 cells using [3H]-thymidine incorporation, immunocapture assay of uPA and tyrosine kinase assay.', 'The growth factors stimulated growth of HCE 16/3 cells in serum-free medium. However, genistein suppressed the growth stimulatory effect of growth factors on HCE 16/3 cells and also reduced KGF-enhanced uPA secretion in this cell line. The inhibitory effects might be associated with its attenuation of activity of tyrosine kinases of growth factors.
Answer: The results suggest that genistein have potential value in the prevention and treatment of some tumors in vivo.

Answer:
Does [ Genistein suppress growth stimulatory effect of growth factors in HCE 16/3 cells ]?