In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

A nested PCR assay was used to determine the viral RNA and proviral DNA status of naturally infected cats. Selected samples that were FeLV-positive by PCR were subjected to sequencing, phylogenetic analysis, and motifs search. Of the 39 samples that were positive for FeLV p27 antigen, 87.2% (34/39) were confirmed positive with nested PCR. FeLV proviral DNA was detected in 38 (97.3%) of p27-antigen negative samples. Malaysian FeLV isolates are found to be highly similar with a homology of 91% to 100%. Phylogenetic analysis revealed that Malaysian FeLV isolates divided into two clusters, with a majority (86.2%) sharing similarity with FeLV-K01803 and fewer isolates (13.8%) with FeLV-GM1 strain. Different enhancer motifs including NF-GMa, Krox-20/WT1I-del2, BAF1, AP-2, TBP, TFIIF-beta, TRF, and TFIID are found to occur either in single, duplicate, triplicate, or sets of 5 in different positions within the U3-LTR-gag region. The present result confirms the occurrence of FeLV viral RNA and provirus DNA in naturally infected cats. Malaysian FeLV isolates are highly similar, and a majority of them are closely related to a UK isolate. This study provides the first molecular based information on FeLV in Malaysia. Additionally, different enhancer motifs likely associated with FeLV related pathogenesis have been identified.
Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia

The cellular role of DNA is relatively limited, perhaps because of the restrictions imposed by bonding between complementary strands. Outside the cell, however, nanoengineers are uncovering the many hidden talents of DNA. The DNA sequence is able to process information in biochemical assays, its structure is an ideal building material, and its folding pathway allows DNA to move and respond to its environment. Here, I review several innovative applications of designed DNA molecules, some underlying design principles and the prospects for future developments. The applications of new DNA-based technologies range from molecular diagnostics to protein purification and from therapeutics to the assembly of tiny electronic circuits. These uses exploit the properties of DNA at three levels -sequence only, structure (which depends on sequence) and folding pathways (which depend on both sequence and structure) -as the following examples show. By taking their cue from the genetic code found in nature, nanoengineers are now using DNA sequences in vitro to direct the synthesis and evolution of molecules with new functions and reactive properties. When attached to other molecules, detection methods that exploit DNA sequences allow complementary molecules to be identified at extremely low concentrations. DNA sequences can also be used, in a non-biological setting, to control the layout of electronic components in a nanocircuit. DNA strands can fold into stable structures that have valuable functional and material properties. Sensitive methods for detecting DNA have been extended to detect proteins and other macromolecular targets of DNA aptamers -that is, nucleic-acid molecules that have high binding specificity for their targets. Lattices and even three-dimensional (3D) objects have been self-assembled from rigid DNA structures. DNA lattices can organize proteins and nanoelectronic components on a surface with unprecedented precision, making it possible to gain a better understanding of the properties and interactions of proteins. The folding pathway of a DNA molecule to its stable structure allows it to move and perform mechanical functions. The energy released in DNA-folding pathways has been used in vitro to drive motors, providing the ability to release, grab or cleave target molecules and even to control the release of drugs, depending on the outcome of certain diagnostic tests. This review focuses on the recent developments in generating rationally designed DNA molecules and their applications. The past decade has seen huge advances in the development of design principles, particularly by exploiting DNA structure and folding pathways, but also in applications of designed DNA sequences. Together, techniques for the in vitro selection or molecular evolution of DNA molecules from random pools have given spectacular yields, including DNA aptamers and enzymes, which can inhibit the expression of harmful genes or disrupt protein function. Excellent reviews of selected DNA molecules can be found elsewhere 1-3 ; this paper includes only examples of designed DNA molecules, or molecules that combine both selected and designed Abstract | Long admired for its informational role in the cell, DNA is now emerging as an ideal molecule for molecular nanotechnology. Biologists and biochemists have discovered DNA sequences and structures with new functional properties, which are able to prevent the expression of harmful genes or detect macromolecules at low concentrations. Physical and computational scientists can design rigid DNA structures that serve as scaffolds for the organization of matter at the molecular scale, and can build simple DNA-computing devices, diagnostic machines and DNA motors. The integration of biological and engineering advances offers great potential for therapeutic and diagnostic applications, and for nanoscale electronic engineering.
Designed DNA molecules: principles and applications of molecular nanotechnology

There was a pandemic of influenza A (H1N1) in 2009; in Korea, there was also an H1N1 epidemic in 2016. We aim to investigate whether survival had improved in the setting of recent advances in intensive care unit (ICU) management. We conducted a retrospective analysis of acute respiratory failure patients with H1N1 influenza pneumonia in 2016 and 2009 respectively at two tertiary referral hospitals in Korea. A total of 28 patients were treated in 2016, and 34 in 2009. There was no significant difference in SOFA scores on ICU admission day. In-hospital mortality was significantly lower in patients of 2016 compared to those of 2009 (18% vs. 44% P = 0.028). By multivariable analyses, the treatment year 2016 was associated with a greater likelihood of survival. Compared to the patients treated in 2009, those treated in 2016 were one seventh as likely to die after adjusting for other clinical variables (hazard ratio for mortality, 0.15; 95% confidence interval. 0.03-0.63, P = 0.010). Improved survival in patients who underwent extracorporeal membrane oxygenation treatment (in-hospital mortality, 17% vs. 60%, P = 0.242) and decreased tidal volumes during mechanical ventilation (median 5.4 mL/kg vs. median 9.2 mL/kg, P = 0.018) were observed in

Improved survival rates in patients with H1N1 acute respiratory failure in Korea between 2009 and 2016