In this task, you are given a multiple-choice question about healthcare. Answer the question based on your information and classify your answers into '1', '2', '3', and '4'.

Q: Question: The process of bacterial sporulation occurs: 
 Options:  <1> Both Gram positive and Gram negative bacteria. <2> After the depletion of certain essential nutrients. <3> When unfavorable environmental factors such as UV light or heat appear. <4> During the exponential growth. <5> By budding of a stem cell.

A: 2
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Q: Question: Sulfasalazine is used for the treatment of ulcerative colitis. It is a prodrug of 5-aminosalicylic acid that is activated by a process of: 
 Options:  <1> Hydrolysis of an ester. <2> Hydrolysis of an amide. <3> Oxidative dealkylation. <4> Reducing rupture of the azoderivative. <5> Aromatic hydroxylation.

A: 4
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Q: Question: The negative feedback regulation of thyroid hormone is as follows: 
 Options:  <1> High concentrations of circulating free T3 and T4 that elevate HRT. <2> High concentrations of circulating free T3 and T4 that raise TSH. <3> High concentrations of circulating free T3 and T4 that decrease TRH. <4> Decreased TRH that elevates TSH. <5> Decreased TSH that elevates HRT.

A:
3
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