instruction:
In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".
question:
Paragraph: We have developed a full genome virus detection process that combines sensitive nucleic acid preparation optimised for virus identification in fecal material with Illumina MiSeq sequencing and a novel post-sequencing virus identification algorithm. Enriched viral nucleic acid was converted to double-stranded DNA and subjected to Illumina MiSeq sequencing. The resulting short reads were processed with a novel iterative Python algorithm SLIM for the identification of sequences with homology to known viruses. De novo assembly was then used to generate full viral genomes. The sensitivity of this process was demonstrated with a set of fecal samples from HIV-1 infected patients. A quantitative assessment of the mammalian, plant, and bacterial virus content of this compartment was generated and the deep sequencing data were sufficient to assembly 12 complete viral genomes from 6 virus families. The method detected high levels of enteropathic viruses that are normally controlled in healthy adults, but may be involved in the pathogenesis of HIV-1 infection and will provide a powerful tool for virus detection and for analyzing changes in the fecal virome associated with HIV-1 progression and pathogenesis. 
 Title: Full Genome Virus Detection in Fecal Samples Using Sensitive Nucleic Acid Preparation, Deep Sequencing, and a Novel Iterative Sequence Classification Algorithm
answer:
False


question:
Paragraph: Seasonal hyperacute panuveitis (SHAPU) is a potentially blinding ocular disease occurring in Nepal that principally affects young children. Random amplification of partially purified vitreous fluid (VF)-derived nucleic acid revealed the presence of human anelloviruses in VF of SHAPU patients. In a comparative study of patients with different ocular pathologies, SHAPU patients were at highest risk of harboring anelloviruses in their eyes. The majority of SHAPU patients had multiple anelloviruses in their VF. The ocular anellovirus load in SHAPU and non-SHAPU patients did not differ and no SHAPU-specific anellovirus variant was detected. Analysis of paired serum and VF samples from SHAPU and non-SHAPU patients showed that the anellovirus detected in VF samples most likely originated from the systemic viral pool during viremia, potentially through breakdown of the blood-ocular barrier. The detection of anelloviruses in VF samples of uveitis patients, profoundly so in SHAPU patients, is imperative and warrants elucidation of its clinical significance. 
 Title: High Prevalence of Anelloviruses in Vitreous Fluid of Children With Seasonal Hyperacute Panuveitis
answer:
True


question:
Paragraph: Bacterial extracellular nucleases play important roles in virulence, biofilm formation, utilization of extracellular DNA as a nutrient, and degradation of neutrophil DNA extracellular traps. However, there is no current data available for extracellular nucleases derived from M. tuberculosis. Herein, we have identified and characterized Rv0888, an extracellular nuclease in M. tuberculosis. The protein was overexpressed in E. coli, and the purified Rv0888 protein was found to require divalent cations for activity, with an optimal temperature and pH of 41 °C and 6.5, respectively. Further results demonstrated that Rv0888 nuclease activity could be inhibited by four Chinese medicine monomers. Based on sequence analysis, Rv0888 nuclease exhibited no homology with any known extracellular nucleases, indicating that Rv0888 is a novel nuclease. Site-directed mutagenesis studies revealed that the H353, D387, and D438 residues play catalytic roles in Rv0888. In vivo infection studies confirmed that Rv0888 is required for infection and is related to pathogenicity, as the persistent ability of recombinant Mycobacterium smegmatis (rMS) Rv0888NS/MS and Rv0888S/MS is significantly higher than pMV262/MS in the lung tissue, and the Rv0888NS/MS and Rv0888S/MS could produce pathological changes in the mice lung. These results show that Rv0888 is relevant to pathogenicity of M. tuberculosis. A variety of bacteria produce extracellular DNases/nucleases that are important for virulence, biofilm formation, utilization of extracellular DNA as a nutrient, and degradation of neutrophil DNA extracellular traps (NETs). Extracellular nucleases produced by Shewanella, Serratia marcescens, Staphylococcus aureus allow these bacteria to degrade extracellular DNA to its basic components: phosphate, carbon, and nitrogen 1-3 . A recent study by Seper et al. demonstrated that wild-type Vibrio cholerae rapidly degraded the DNA component of NETs through the combined activity of two extracellular nucleases, Dns and Xds 4 . In S. aureus, an extracellular nuclease also degraded NETs, triggering caspase-3-mediated death of immune cells 5 . Moreover, nuclease A (Gbs0661), an extracellular nuclease of Streptococcus agalactiae, is required for full virulence of the organism 6 and attacks NETs, which are a component of the host defense response that immobilizes microbes for subsequent clearance by innate immunity machinery, including phagocytosis by macrophages. A Mn 2+ -stimulated deoxyribonuclease (Nuc B) is released by Bacillus subtilis 168 during sporulation in glucose-deficient medium, which degrades structurally important nucleic acids, thereby controlling the development and dispersal of bacterial biofilms 7,8 . Mycobacterium tuberculosis (M. tuberculosis) is a Gram-positive bacterium that causes tuberculosis (TB), which remains one of the world's deadliest communicable diseases. In 2013, an estimated 9.0 million people contracted TB and a subsequent 1.5 million people died from the disease, of whom 360,000 were HIV-positive 9 . M. tuberculosis is phagocytosed by alveolar macrophages and dendritic cells after inhalation into the lung. However, M. tuberculosis can proliferate within these immune cells, eventually escaping from the phagosome and migrating to draining lymph nodes to spread the infection 10-12 . Rv0888, a protein that belongs to the large endonuclease/exonuclease/phosphatase family (Pfam family PF03372) 13 , has sphingomyelinase activity that has been detected in M. tuberculosis culture filtrates 14 . In this study, we identified and characterized Rv0888, the first extracellular nuclease to be reported from M. tuberculosis. We demonstrate that this enzyme is highly active in degrading different types of nucleic acids (chromosomal DNA, double-stranded PCR products, plasmid DNA, and baker's yeast RNA) utilizing divalent metal cations (Ca 2+ and Mn 2+ ). In addition, the nuclease activity of Rv0888 is inhibited by four Chinese medicine monomers (Oleuropein, 6-Gingerol, Corylifolinin, and Acteoside). Finally, we confirmed that Rv0888 is relevant to mycobacterial pathogenicity. 
 Title: Characterization of Rv0888, a Novel Extracellular Nuclease from Mycobacterium tuberculosis OPEN
answer:

False