In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Example Input: Coxsackieviruses are enteric viruses that frequently infect humans. To examine coxsackievirus pathogenesis, we orally inoculated mice with the coxsackievirus B3 (CVB3) Nancy strain. Using HeLa cell plaque assays with agar overlays, we noticed that some fecal viruses generated plaques >100 times as large as inoculum viruses. These large-plaque variants emerged following viral replication in several different tissues. We identified a single amino acid change, N63Y, in the VP3 capsid protein that was sufficient to confer the large-plaque phenotype. Wild-type CVB3 and N63Y mutant CVB3 had similar plaque sizes when agarose was used in the overlay instead of agar. We determined that sulfated glycans in agar inhibited plaque formation by wildtype CVB3 but not by N63Y mutant CVB3. Furthermore, N63Y mutant CVB3 bound heparin, a sulfated glycan, less efficiently than wild-type CVB3 did. While N63Y mutant CVB3 had a growth defect in cultured cells and reduced attachment, it had enhanced replication and pathogenesis in mice. Infection with N63Y mutant CVB3 induced more severe hepatic damage than infection with wild-type CVB3, likely because N63Y mutant CVB3 disseminates more efficiently to the liver. Our data reinforce the idea that culture-adapted laboratory virus strains can have reduced fitness in vivo. N63Y mutant CVB3 may be useful as a platform to understand viral adaptation and pathogenesis in animal studies. IMPORTANCE Coxsackieviruses frequently infect humans, and although many infections are mild or asymptomatic, there can be severe outcomes, including heart inflammation. Most studies with coxsackieviruses and other viruses use laboratory-adapted viral strains because of their efficient replication in cell culture. We used a cell culture-adapted strain of CVB3, Nancy, to examine viral replication and pathogenesis in orally inoculated mice. We found that mice shed viruses distinct from input viruses because they formed extremely large plaques in cell culture. We identified a single mutation, VP3 N63Y, that was sufficient for large-plaque formation. N63Y mutant viruses have reduced glycan binding and replication in cell culture; however, they have enhanced replication and virulence in mice. We are now using N63Y mutant CVB3 as an improved system for viral pathogenesis studies. Citation Wang Y, Pfeiffer JK. 2016. Emergence of a large-plaque variant in mice infected with coxsackievirus B3. mBio 7(2):e00119-16.
Example Output: Emergence of a Large-Plaque Variant in Mice Infected with Coxsackievirus B3

Example Input: Arenaviruses are a family of enveloped RNA viruses that cause severe human disease. The first step in the arenavirus life cycle is attachment of viral particles to host cells. While virus-cell attachment can be measured through the use of virions labeled with biotin, radioactive isotopes, or fluorescent dyes, these approaches typically require high multiplicities of infection (MOI) to enable detection of bound virus. We describe a quantitative (q)RT-PCR-based assay that measures Junin virus strain Candid 1 attachment via quantitation of virion-packaged viral genomic RNA. This assay has several advantages including its extreme sensitivity and ability to measure attachment over a large dynamic range of MOIs without the need to purify or label input virus. Importantly, this approach can be easily tailored for use with other viruses through the use of virus-specific qRT-PCR reagents. Further, this assay can be modified to permit measurement of particle endocytosis and genome uncoating. In conclusion, we describe a simple, yet robust assay for highly sensitive measurement of arenavirus-cell attachment. The video component of this article can be found at http://www.jove.com/video/53682/ 15, 23 . The qRT-PCR-based assay described here can detect attachment events using as few as 20 plaque forming units (PFUs) of virus, which translates into an MOI of 0.0004 for a 48-well plate. Therefore, the strong sensitivity of the assay overcomes the requirement for high MOI as well as any virus labeling or concentration steps. Further, the assay can be i) adapted to measure virion endocytosis Journal of Visualized Experiments
Example Output: Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Example Input: Please cite this article as: A. Elkbuli, H. Ehrlich and M. McKenney, The effective use of telemedicine to save lives and maintain structure in a healthcare system: Current response to COVID-1, American Journal of Emergency Medicine (2020), https://doi.Journal Pre-proof J o u r n a l P r e -p r o o f The Effective Use of Telemedicine to Save Lives and Maintain Structure in a Healthcare System: Current Response to COVID-19 Journal Pre-proof J o u r n a l P r e -p r o o f Throughout the response to the COVID19 crisis, many healthcare institutions have increased their use of telecommunications. From medical schools to residency programs, from patient interactions at home to those in quarantine, virtual communication is providing a safe way to continue with our responsibilities during this pandemic. According to POTUS (17 March 2020), telemedicine, with lessened HIPAA regulations, is now an approved method of communication between physicians and patients for those with Medicare. 1 Telemedicine provides protection to both the physician and patient, preventing possible spread of COVID-19 while allowing for continued patient care. It is now vital to make practical, effective use of telecommunications to stabilize the healthcare system. Recently, medical schools have transferred their curriculum to continue online. With the AAMC recommending a "temporary suspension of medical students' participation in any activities that involve patient(s)" virtual education allows for continued development, while still adhering to social distancing. 2,3 Web conferencing has features such as audio-video, screen sharing, large login capacities and chat functions, allowing students and educators to maintain their regular teaching schedule. 3 The use of telecommunications is indispensable to disseminate knowledge and scientific updates and to swiftly overcome the disruptions in scientific conferences and meetings. Telecommunication and virtual learning can provide medical education and training for those with adjusted or challenging schedules due to the demands of the pandemic. 3,4 Virtual learning not only can be but should be utilized for morning report, sign-out, M&M, and communicating with staff in high risk areas without fear of increasing risk of COVID-19 exposure. Social distancing does not need to be at the expense of effective communication, which itself is fundamental to address this crisis efficiently and effectively. Journal Pre-proof
Example Output:
Journal Pre-proof The effective use of telemedicine to save lives and maintain structure in a healthcare system: Current response to COVID-1 The Effective Use of Telemedicine to Save Lives and Maintain Structure in a Healthcare System: Current Response to COVID-19