In this task, you are given a multiple-choice question about healthcare. Answer the question based on your information and classify your answers into '1', '2', '3', and '4'.

[Q]: Question: One of the following types of transport through membranes does NOT exist: 
 Options:  <1> Ion transport facilitated by ionophore (against electrochemical gradient). <2> Facilitated diffusion (in favor of electrochemical gradient) <3> Primary active transport (against electrochemical gradient). <4> Ionic channel (in favor of electrochemical gradient). <5> Simple diffusion (in favor of concentration gradient).
[A]: 1


[Q]: Question: The ribosomal subunits are formed in the: 
 Options:  <1> Nucleoplasm. <2> Nucleolus <3> Nuclear sheet. <4> Nuclear pore. <5> Cytoplasm.
[A]: 2


[Q]: Question: How does the increase in temperature affect the reaction between antigen and antibody? 
 Options:  <1> Increases the reaction rate and decreases binding affinity. <2> It decreases the reaction rate and increases the binding affinity. <3> Increases the reaction rate and binding affinity. <4> Decreases the reaction rate and binding affinity. <5> None of the above options is correct.
[A]:
1