Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

Ex Input:
1. In order to investigate the biological function of the human CLN3 gene that is defective in Batten disease, we created a yeast strain by PCR-targeted disruption of the yeast gene (YHC3), which is a homologue of the human CLN3 gene. 2. The phenotypic characterization revealed that the yhc3 delta mutants are more sensitive to combined heat and alkaline stress than the wild-type strains as determined by inhibition of cell proliferation. 3. This suggests that the yhc3 delta mutant is a good model to investigate the biological function of human CLN3 gene in mammalian cells and to understand the pathophysiology of juvenile Batten disease.

Ex Output:
What is the effect of a defective CLN3 gene?


Ex Input:
Mr. Flip is a 1909 American silent comedy film made by Essanay Studios, directed by Gilbert M. 'Broncho Billy' Anderson and starring Ben Turpin.

Ex Output:
What production company was involved in Mr. Flip?


Ex Input:
An Alexanderson alternator is a rotating machine invented by Ernst Alexanderson in 1904 for the generation of high-frequency alternating current for use as a radio transmitter.

Ex Output:

Who found Alexanderson alternator?