In this task, you are given a multiple-choice question about healthcare. Answer the question based on your information and classify your answers into '1', '2', '3', and '4'.

[EX Q]: Question: Protein kinase A (PKA): 
 Options:  <1> It induces the synthesis of glycogen in response to an increase in glucagon in the blood. <2> It is a membrane protein that is activated in response to an increase in circulating GTP. <3> It is activated through the cAMP produced in response to an increase in adrenaline in the circulation. <4> It inhibits the lipolysis of triacylglycerides accumulated in adipose tissue, in response to an increase in intracellular cAMP. <5> It is a monomeric protein that inhibits the synthesis of ATP and muscle contraction.
[EX A]: 3

[EX Q]: Question: If you want to estimate the effects of an intervention, you will use: 
 Options:  <1> A transversal design. <2> A retrospective design <3> An ecological study. <4> A randomized clinical trial. <5> An observational design with random selection of the participants.
[EX A]: 4

[EX Q]: Question: The denaturation of proteins: 
 Options:  <1> It is accompanied by the loss of their activity. <2> It supposes the rupture of peptide bonds. <3> It is always an irreversible process. <4> Alter its primary structure. <5> It is carried out by chaperone proteins.
[EX A]:
1