In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

The dog is considered the main domestic reservoir for Trypanosoma cruzi infection and a suitable experimental animal model to study the pathological changes during the course of Chagas disease (CD). Vaccine development is one of CD prevention methods to protect people at risk. Two plasmids containing genes encoding a trans-sialidase protein (TcSP) and an amastigote-specific glycoprotein (TcSSP4) were used as DNA vaccines in a canine model. Splenomegaly was not found in either of the recombinant plasmid-immunized groups; however, cardiomegaly was absent in animals immunized only with the plasmid containing the TcSSP4 gene. The inflammation of subendocardial and myocardial tissues was prevented only with the immunization with TcSSP4 gene. In conclusion, the vaccination with these genes has a partial protective effect on the enlargement of splenic and cardiac tissues during the chronic CD and on microscopic hearth damage, since both plasmids prevented splenomegaly but only one avoided cardiomegaly, and the lesions in heart tissue of dog immunized with plasmid containing the TcSSP4 gene covered only subepicardial tissue.
Effect of the Plasmid-DNA Vaccination on Macroscopic and Microscopic Damage Caused by the Experimental Chronic Trypanosoma cruzi Infection in the Canine Model

Background: Fevers of unknown origin constitute a substantial disease burden in Southeast Asia. In majority of the cases, the cause of acute febrile illness is not identified. Methods: We used MassTag PCR, a multiplex assay platform, to test for the presence of 15 viral respiratory agents from 85 patients with unexplained respiratory illness representing six disease clusters that occurred in Cambodia between 2009 and 2012. Results: We detected a virus in 37 (44%) of the cases. Human rhinovirus, the virus detected most frequently, was found in both children and adults. The viruses most frequently detected in children and adults, respectively, were respiratory syncytial virus and enterovirus 68. Sequence analysis indicated that two distinct clades of enterovirus 68 were circulating during this time period. Conclusions: This is the first report of enterovirus 68 in Cambodia and contributes to the appreciation of this virus as an important respiratory pathogen.
Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia

The management of comprehensive and extensive, multi-lateral and multi-national programmes and projects in the field of coastal and ocean management poses numerous organisational problems encompassing co-ordination between: the actions of individual participating countries at the regional level; the national level actions of institutions from different sectors; and actions that are designed to address issues as diverse as: biological diversity conservation and sustainable use; fisheries management; maritime transport; and the control of land based pollution. Most large multi-lateral projects focus on sound scientific knowledge and information, and pay less attention to the design of a management structure that will ensure coherence and co-ordination of the interventions once the programme or project is under implementation. The project entitled "Reversing Environmental Degradation Trends in the South China Sea and Gulf of Thailand" was complex since it addressed three priority areas of concern namely the loss and degradation of coastal habitats, over-exploitation of fisheries in the Gulf of Thailand, and land-based pollution. It is suggested that the success of the management framework reflects the following key design elements: The framework permitted and encouraged both "horizontal" (inter-country) and "vertical" (intra-country) interactions and networking between individuals at all levels of project implementation and execution; Inclusion of a body (the Regional Scientific and Technical Committee) that served as a forum for reconciling both sectorial and national interests and priorities; The clear separation between discussions of scientific and technical matters from discussions dealing with policy and principles at both the national and regional levels; The framework facilitated the incorporation of sound scientific and technical advice and information into politically based decision-making; The use of regional experts and consultants from the participating countries fostered "ownership" of the activities and outputs; Restriction of the membership of the Project Steering Committee to government representatives only, and exclusion of observers from regional and international agencies and institutions other than UNEP; and, The framework allowed for adaptive management and was not a rigid unchanging structure.

Managing multi-lateral, intergovernmental projects and programmes: the case of the UNEP/GEF South China Sea project