instruction:
Given a passage, construct a question on the basis of the information present in the passage. Construct the question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) it answer uses text phrases from the passage. Avoid creating questions that (i) can't be answered correctly without actually understanding the passage and (ii) uses the same words or phrases given in the passage.
question:
The objective of this study is to characterize differential proteomic expression among well-differentiation and poor-differentiation colorectal carcinoma tissues and normal mucous epithelium.', 'The study is based on quantitative 2-dimensional gel electrophoresis and analyzed by PDquest.', 'Excluding redundancies due to proteolysis and posttranslational modified isoforms of over 600 protein spots, 11 proteins were revealed as regulated with statistical variance being within the 95th confidence level and were identified by peptide mass fingerprinting in matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Progression-associated proteins belong to the functional complexes of tumorigenesis, proliferation, differentiation, metabolism, and the regulation of major histocompatibility complex processing and other functions. Partial but significant overlap was revealed with previous proteomics and transcriptomics studies in CRC. Among various differentiation stage of CRC tissues, we identified calreticulin precursor, MHC class I antigen (human leukocyte antigen A ), glutathione S-transferase pi1, keratin 8, heat shock protein 27, tubulin beta chain, triosephosphate, fatty acid-binding protein, hemoglobin (deoxy) mutant with val b 1 replaced by met (HBB), and zinc finger protein 312 (FEZF2).
answer:
Does proteomic analysis reveal novel proteins associated with progression and differentiation of colorectal carcinoma?


question:
Neurons have a very high energy requirement, and their metabolism is tightly regulated to ensure delivery of adequate substrate to sustain neuronal activity and neuroplastic changes. The mechanisms underlying the regulation of neuronal metabolism, however, are not completely clear.', 'The objective of this study was to investigate the central carbon metabolism in neurons, in order to identify the regulatory pathways governing neuronal anabolism and catabolism.', 'Here we first have applied MS-based endometabolomics to elucidate the metabolic dynamics in cultured hippocampal primary neurons. Using nanoLC-ESI-LTQ Orbitrap MS approach followed by statistical analysis, we measure the dynamics of uniformly labeled ', 'According to non-parametric statistical analysis of metabolic dynamics, in cultured hippocampal neurons, the glycerol phosphate shuttle is active and correlates with the metabolic flux in the pentose phosphate pathway. In the hippocampus, glycerol-3-phosphate biosynthesis was activated in response to long-term potentiation together with the upregulation of glycolysis and the TCA cycle, but was inactive or silenced in basal conditions.
answer:
Is biosynthesis of glycerol phosphate associated with long-term potentiation in hippocampal neurons?


question:
Glycemic load represents the total glycemic effect of the diet and may reduce the risk for chronic disease by affecting the risk for obesity and by altering metabolic endpoints. The food choices associated with lower-glycemic-load diets have received little investigation. Therefore, the purpose of this research was to examine the food patterns associated with lower-glycemic-load diets to establish targeted intervention messages.', 'A random sample (n=179; 81 male and 98 female subjects) of older adults > or =65 years of age in the Geisinger Rural Aging study, a nutritional risk screening study.', 'Standardized methodology was used to calculate the glycemic load from data obtained in five 24-hour recalls. Statistical analysis t tests compared dietary patterns between male and female subjects from two eating pattern clusters identified in previous cluster analysis based on food group intake.', 'The mean (+/-standard deviation) glycemic load for the entire sample was 115.6 (+/-39.9). Two clusters were identified, and male and female subjects in one cluster had a lower glycemic load (113.7+/-44.2 and 94.0+/-27.5, respectively) than male and female subjects in the second cluster (139.9+/-38.8 and 110.7+/-35.9, respectively) ( P <.01). Participants in the lower-glycemic-load cluster consumed more carbohydrate from cereal, fruits, vegetables, and milk, whereas those in the higher-glycemic-load cluster consumed more breads and desserts.
answer:

Are more favorable dietary patterns associated with lower glycemic load in older adults?