Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

[Q]: In eukaryotic cells, there are two sub-pathways of nucleotide excision repair (NER), the global genome (gg) NER and the transcription-coupled repair (TCR). TCR can preferentially remove the bulky DNA lesions located at the transcribed strand of a transcriptional active gene more rapidly than those at the untranscribed strand or overall genomic DNA. This strand-specific repair in a suitable restriction fragment is usually determined by alkaline gel electrophoresis followed by Southern blotting transfer and hybridization with an indirect end-labeled single-stranded probe. Here we describe a new method of TCR assay based on strand-specific-PCR (SS-PCR). Using this method, we have investigated the role of DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase-related protein kinases (PIKK) family, in the TCR pathway of UV-induced DNA damage. Although depletion of DNA-PKcs sensitized HeLa cells to UV radiation, it did not affect the ggNER efficiency of UV-induced cyclobutane pyrimidine dimers (CPD) damage. We postulated that DNA-PKcs may involve in the TCR process. To test this hypothesis, we have firstly developed a novel method of TCR assay based on the strand-specific PCR technology with a set of smart primers, which allows the strand-specific amplification of a restricted gene fragment of UV radiation-damaged genomic DNA in mammalian cells. Using this new method, we confirmed that siRNA-mediated downregulation of Cockayne syndrome B resulted in a deficiency of TCR of the UV-damaged dihydrofolate reductase (DHFR) gene. In addition, DMSO-induced silencing of the c-myc gene led to a decreased TCR efficiency of UV radiation-damaged c-myc gene in HL60 cells. On the basis of the above methodology verification, we found that the depletion of DNA-PKcs mediated by siRNA significantly decreased the TCR capacity of repairing the UV-induced CPDs damage in DHFR gene in HeLa cells, indicating that DNA-PKcs may also be involved in the TCR pathway of DNA damage repair. By means of immunoprecipitation and MALDI-TOF-Mass spectrometric analysis, we have revealed the interaction of DNA-PKcs and cyclin T2, which is a subunit of the human transcription elongation factor (P-TEFb). While the P-TEFb complex can phosphorylate the serine 2 of the carboxyl-terminal domain (CTD) of RNA polymerase II and promote transcription elongation. A new method of TCR assay was developed based the strand-specific-PCR (SS-PCR). Our data suggest that DNA-PKcs plays a role in the TCR pathway of UV-damaged DNA. One possible mechanistic hypothesis is that DNA-PKcs may function through associating with CyclinT2/CDK9 (P-TEFb) to modulate the activity of RNA Pol II, which has already been identified as a key molecule recognizing and initializing TCR.
[A]: Which gene strand is targeted by transcription-coupled repair (TCR)?


[Q]: Jan Muhlstein (born 3 July 1949 in Most, Czechoslovakia) is a journalist, German Jewish activist and the former chair of the Union of Progressive Jews in Germany.
[A]: Which language or languages is Jan Mühlstein fluent in?


[Q]: If prefix = st1 /U.S.software companies don't pay more attention to quality, they could kiss their business good-bye. BothIndiaandBrazilare developing a world-class software industry. Their weapon is quality and one of their jobs is to attract the topU.S.quality specialists whose voices are not listened to in their country. Already, of the world's 12 software houses that have earned the highest rating in the world, seven are in India. That's largely because they have used new methods rejected by American software specialists. For example, for decades, quality specialists, W. Edwards Deming and J. M. Juran had urgedU.S.software companies to change their attitudes to quality. But their quality call mainly fell on deaf ears in the U.S -- but not in Japan. By the 1970s and 1980s,Japanwas grabbing market share with better, cheaper products. They used Deming's and Juran's ideas to bring down the cost of good quality to as little as 5% of total production costs. InU.S.factories, the cost of quality then was 10 times as high: 50%. In software, it still is. Watts S. Humphrey spent 27 years at IBM heading up software production and then quality assurance. But his advice was seldom paid attention to. He retired from IBM in 1986. In 1987, he worked out a system for assessing(evaluating) and improving software quality. It has proved its value time and again. For example, in 1990 the cost of quality at Raytheon Electronics Systems was almost 60% of total software production costs. It fell to 15% in 1996 and has since further dropped to below 10%. Like Deming and Juran, Humphrey seems to be winning more praises overseas than at home. The Indian government and several companies have just founded the Watts Humphrey Software Quality Institute at the Software Technology Park in Chennai,India. Let's hope thatU.S.lead in software will not be eaten up by its quality problems.
[A]:
What country has more highest-rating companies in the world than any other country has?