Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.
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Question: Mystery, Inc. goes their separate ways after becoming bored of mystery solving. Daphne Blake, along with Fred Jones, starts running a successful television series. Velma Dinkley becomes the proprietor of a mystery bookstore, and Scooby-Doo and his owner Shaggy Rogers bounce from job to job. For Daphne's birthday, Fred decides to get the gang back together for a road trip while Daphne is filming her show. After encountering a lot of fake monsters, the gang finally arrives in New Orleans. They are soon invited by a young woman named Lena Dupree to visit Moonscar Island, her employer's home, which is allegedly haunted by the ghost of the pirate Morgan Moonscar. Although the gang is skeptical, they go with Lena, to whom Fred has taken a fancy. On the island, the gang meets Lena's employer Simone Lenoir as well as the ferryman Jacques and Simone's gardener Beau, to whom Daphne takes a fancy. They also meet Snakebite Scruggs, a ill-natured fisherman, and his hunting pig, Mojo. The gang sets out to prove that the "ghost" is a fake. Scooby and Shaggy are chased by Mojo and end up falling into a big hole, where they encounter the zombie of Morgan Moonscar. By the time the rest of gang comes to investigate, Moonscar isn't around. Simone invites the gang to her house to stay for the night. As the gang is dressing up for dinner, Shaggy sees the ghost of a Confederate colonel in the mirror; Simone explains that the island was a temporary headquarters for a Confederate regiment during the American Civil War. Due to Simone's cats, Scooby and Shaggy eat in the Mystery Machine, but find the food spicy and get some water from the lake, where an army of zombies emerge. Shaggy's bad driving gets the Mystery Machine stuck in the mud, forcing him and Scooby to flee on foot. Fred and Daphne find the Mystery Machine, but no sign of Shaggy and Scooby. They argue about each other's supposed love interests and come across Scooby and Shaggy. They manage to capture a zombie, which is revealed to be real. As the zombies swarm around them, the gang splits in panic. Elsewhere, Scooby and Shaggy discover wax dolls that look like Fred, Velma, and Daphne, and they play with them, causing their friends to undertake a series of involuntary actions for a short time until they leave after disturbing a nest of bats. Fred, Daphne, Velma, and Beau return to Simone's house and discover a secret passage under the staircase. They find Lena, who tells them that the zombies took Simone away. Fred, Daphne, Velma, Beau, and Lena find a secret chamber for voodoo rituals, where Velma finds footprints of Simone's heels and interrogates Lena about the story. Simone then appears, and she and Lena use voodoo dolls to trap the gang in the chamber. They reveal themselves to be evil cat creatures. Simone tells them that several centuries ago, she and Lena were part of a group of settlers who were devoted to a cat god. The vengeful Lena and Simone asked their cat god to curse pirates, who had chased the settlers into the bayou. Their wish was granted and they killed the pirates, but the curse caused the duo to become cat creatures permanently, requiring that they drain life forces to preserve their immortality. They also gave Jacques immortality so they had a ferryman to bring them more victims. The zombies' intent was to warn the gang to leave to escape their fate. Jacques finds and chases Scooby and Shaggy to drain their lives, but they accidentally tumble into the cave, interrupting the draining ceremony and distracting the cat creatures. Velma quickly unties herself and creates voodoo dolls of Lena and Simone to interrupt their ritual. When they are finally cornered, the cat creatures' curse expires, causing them to disintegrate, freeing the zombies' souls to rest in peace. Beau is revealed to be an undercover police officer sent to investigate the disappearances on the island. Daphne offers Beau a chance to guest-star on her show and discuss the adventure. The next morning,

Answer: What is the name of the haunted house that Lena Dupree invites the gang to.


Question: Restless legs syndrome is a neurological disorder characterized by an urgency to move the legs during periods of rest. Data from a variety of sources provide a compelling argument that the amount of iron in the brain is lower in individuals with restless legs syndrome compared with neurologically normal individuals. Moreover, a significant percentage of patients with restless legs syndrome are responsive to intravenous iron therapy. The mechanism underlying the decreased iron concentrations in restless legs syndrome brains is unknown. We hypothesize that the source of the brain iron deficit is at the blood-brain interface. Thus we analysed the expression of iron management proteins in the epithelial cells of the choroid plexus and the brain microvasculature in post-mortem tissues. The choroid plexus, obtained at autopsy, from 18 neurologically normal controls and 14 individuals who had primary restless legs syndrome was subjected to histochemical staining for iron and immunostaining for iron management proteins. Iron and heavy chain ferritin staining was reduced in the epithelial cells of choroid plexus in restless legs syndrome. Divalent metal transporter, ferroportin, transferrin and its receptor were upregulated in the choroid plexus in restless legs syndrome. Microvessels were isolated from the motor cortex of 11 restless legs syndrome and 14 control brains obtained at autopsy and quantitative immunoblot analyses was performed. Expression of heavy chain ferritin, transferrin and its receptor in the microvessels from restless legs syndrome was significantly decreased compared with the controls but divalent metal protein 1, ferroportin, prohepcidin, mitochondrial ferritin and light-chain ferritin remained unchanged. The presence of an iron regulatory protein was demonstrated in the brain microvasculature and the activity of this protein is decreased in restless legs syndrome; a finding similar to our earlier report in neuromelanin cells from the substantia nigra of restless legs syndrome brains. This study reveals that there are alterations in the iron management protein profile in restless legs syndrome compared with controls at the site of blood-brain interface suggesting fundamental differences in brain iron acquisition in individuals with restless legs syndrome. Furthermore, the decrease in transferrin receptor expression in the microvasculature in the presence of relative brain iron deficiency reported in restless legs syndrome brains may underlie the problems associated with brain iron acquisition in restless legs syndrome. The consistent finding of loss of iron regulatory protein activity in restless legs syndrome brain tissue further implicates this protein as a factor in the underlying cause of the iron deficiency in the restless legs syndrome brain. The data herein provide evidence for regulation of iron uptake and storage within brain microvessels that challenge the existing paradigm that the blood-brain barrier is merely a transport system.

Answer: Which deficiency is the cause of restless leg syndrome?


Question: Ehlers-Danlos syndrome is a complex hereditary connective tissue disorder that is characterized by abnormalities of the skin and joints and visceral and neurological manifestations. At present, at least 11 forms are recognized on the basis of their clinical characteristics, methods of transmission, and biochemical defect. The neurologic manifestations include cerebrovascular disease, peripheral neuropathy, plexopathy, periventricular subependymal heterotopias, and epilepsy. Previously, 2 females were reported to be affected with subependimal periventricular heterotopias and Ehlers-Danlos syndrome type 1. The authors report a new case of a 12-year-old girl with similar clinical and neuroradiological features.

Answer:
What tissue is most affected in Ehlers-Danlos syndromes?