TASK DEFINITION: Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.
PROBLEM: Set on the campus of a small New England college, the film focuses on the volatile relationship of a middle-aged couple: associate history professor George (Richard Burton) and his hard-drinking wife Martha (Elizabeth Taylor), the daughter of the college president.It's 2:00 a.m. on a Sunday morning, and they have returned from one of her father's gatherings. Martha announces she has invited a young couple--Nick (George Segal), a young, good-looking, newly appointed instructor, and his mousey wife Honey (Sandy Dennis)--to join them for late-night drinks. George is disturbed because she did so without consulting him first, prompting Martha to launch into the first of many loud and lengthy tirades during which she taunts and criticizes him. Knowing his wife is drunk and quite lewd, he asks her to behave herself when they arrive, and when the doorbell rings, he warns her to refrain from mentioning their child to their company.Overhearing Martha's crude retort as the door opens, Nick and Honey immediately feel ill at ease and quickly find themselves caught in the middle of a verbal war zone when their efforts to engage in small talk set off a volley of insults between their hosts. Martha begins to flirt lewdly with Nick while his meek wife tries to pretend she is unaware of what is happening.While Martha is showing Honey where the bathroom is, George tests Nick's verbal sparring skills, but the young man is no match for his host. Realizing he and his wife are becoming embroiled in the middle of marital warfare, he suggests they depart, but George cajoles him into staying.Upon returning to the living room alone, Honey innocently mentions to George she was unaware he and Martha had a son on the verge of celebrating his 16th birthday. Martha reappears in a new outfit--form-fitting slacks and a revealing blouse--and when her husband makes a snide remark about the ensemble, she begins to demean his abilities as a teacher, then escalates her seduction of Nick, complimenting him on the body he developed as both a quarterback and an intercollegiate state boxing champion while criticizing George's paunch. She informs their guests about a past incident when George refused to engage in a friendly outdoor boxing match with his father-in-law and Martha put on a pair of gloves and punched him in the jaw, knocking him into the bushes. As she relates the story, George aims a shotgun at the back of her head, causing Honey to scream. He pulls the trigger, which releases an umbrella, while he tells his wife she's dead.Honey again raises the subject of George and Martha's son, prompting the couple to engage in a conversation Martha quickly tries to end without success. To counterattack George's relentless comments about the boy, she tells their guests her husband is unsure the child is his own, although he most assuredly is. They argue about the color of the boy's eyes until George threatens to expose the truth about the boy. Furious, Martha accuses him of being a failure whose youthful, idealistic plans for the future slowly deteriorated as he came to realize he wasn't aggressive enough to follow in his father-in-law's footsteps, leaving her stuck with a flop. George cuts the diatribe short by spinning Honey around and mockingly singing, "Who's afraid of Virginia Woolf?", a joke the shrewed Martha had made herself during the party earlier that evening.Inebriated and on the verge of throwing up from George's spinning, Honey rushes from the room. Martha goes to the kitchen to make coffee, and George and Nick go outside. The younger man confesses he was attracted to Honey more for her family's money than passion, and married her only because she mistakenly believed she was pregnant. George describes his own marriage as one of never-ending accommodation and adjustment, then admits he considers Nick a threat. George also tells a story about a boy he grew up with. This boy had accidentally killed his mother. Years later, George claims the boy was driving with his father. He swerved to "miss a porcupine" in the road,

SOLUTION: Who accuses Nick of being sexually inadequate?

PROBLEM: Many different cellular pathways have evolved to protect the genome from the deleterious effects of DNA damage that result from exposure to chemical and physical agents. Among these is a process called transcription-coupled repair (TCR) that catalyzes the removal of DNA lesions from the transcribed strand of expressed genes, often resulting in a preferential bias of damage clearance from this strand relative to its non-transcribed counterpart. Lesions subject to this type of repair include cyclobutane pyrimidine dimers that are normally repaired by nucleotide excision repair (NER) and thymine glycols (TGs) that are removed primarily by base excision repair (BER). While the mechanism underlying TCR is not completely clear, it is known that its facilitation requires proteins used by other repair pathways like NER. It is also believed that the signal for TCR is the stalled RNA polymerase that results when DNA damage prevents its translocation during transcription elongation. While there is a clear role for some NER proteins in TCR, the involvement of BER proteins is less clear. To explore this further, we studied the removal of 7-methylguanine (7MeG) and 3-methyladenine (3MeA) from the dihydrofolate reductase (dhfr) gene of murine cell lines that vary in their repair phenotypes. 7MeG and 3MeA constitute the two principal N-methylpurines formed in DNA following exposure to methylating agents. In mammalian cells, alkyladenine DNA alkyladenine glycosylase (Aag) is the major enzyme required for the repair of these lesions via BER, and their removal from the total genome is quite rapid. There is no observable TCR of these lesions in specific genes in DNA repair proficient cells; however, it is possible that the rapid repair of these adducts by BER masks any TCR. The repair of 3MeA and 7MeG was examined in cells lacking Aag, NER, or both Aag and NER to determine if rapid overall repair masks TCR. The results show that both 3MeA and 7MeG are removed without strand bias from the dhfr gene of BER deficient (Aag deficient) and NER deficient murine cell lines. Furthermore, repair of 3MeA in this region is highly dependent on Aag, but repair of 7MeG is equally efficient in the repair proficient, BER deficient, and NER deficient cell lines. Strikingly, in the absence of both BER and NER, neither 7MeG nor 3MeA is repaired. These results demonstrate that NER, but not TCR, contributes to the repair of 7MeG, and to a lesser extent 3MeA.

SOLUTION: Which gene strand is targeted by transcription-coupled repair (TCR)?

PROBLEM: -Synuclein is an abundant presynaptic protein and a primary component of Lewy bodies in Parkinson disease. Although its pathogenic role remains unclear, in healthy nerve terminals -synuclein undergoes a cycle of membrane binding and dissociation. An -synuclein binding assay was used to screen for vesicle proteins involved in -synuclein membrane interactions and showed that antibodies directed to the Ras-related GTPase Rab3a and its chaperone RabGDI abrogated -synuclein membrane binding. Biochemical analyses, including density gradient sedimentation and co-immunoprecipitation, suggested that -synuclein interacts with membrane-associated GTP-bound Rab3a but not to cytosolic GDP-Rab3a. Accumulation of membrane-bound -synuclein was induced by the expression of a GTPase-deficient Rab3a mutant, by a dominant-negative GDP dissociation inhibitor mutant unable to recycle Rab3a off membranes, and by Hsp90 inhibitors, radicicol and geldanamycin, which are known to inhibit Rab3a dissociation from membranes. Thus, all treatments that inhibited Rab3a recycling also increased -synuclein sequestration on intracellular membranes. Our results suggest that membrane-bound GTP-Rab3a stabilizes -synuclein on synaptic vesicles and that the GDP dissociation inhibitorHsp90 complex that controls Rab3a membrane dissociation also regulates -synuclein dissociation during synaptic activity.

SOLUTION:
Which is the primary protein component of Lewy bodies?