Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

Q: Malaria, which is the result of Plasmodium falciparum infection, is a global health threat that resulted in 655,000 deaths and 216 million clinical cases in 2010 alone. Recent phase 3 trials with malaria vaccine candidate RTS,S/AS01 (RTS,S) in children has demonstrated modest efficacy against clinical and severe malaria. RTS,S targets the pre-erythrocytic phase of the disease and induces high antibody titers against the P. falciparum circumsporozoite protein (CSP) and a moderate CD4(+) T cell response. The individual contribution of these adaptive immune responses to protection from infection remains unknown. Here, we found that prophylactic administration of anti-CSP mAbs derived from an RTS,S-vaccinated recipient fully protected mice with humanized livers from i.v.- and mosquito bitedelivered P. falciparum sporozoite challenge. Titers of anti-CSP that conveyed full protection were within the range observed in human RTS,S vaccine recipients. Increasing anti-CSP titers resulted in a dose-dependent reduction of the liver parasite burden. These data indicate that RTS,S-induced antibodies are protective and provide sterilizing immunity against P. falciparum infection when reaching or exceeding a critical plasma concentration.

A: RTS S AS01 vaccine was developed to prevent which disease?
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Q: The film starts on June 16, 1994 with Jesse meeting Celine on a train from Budapest and striking up a conversation with her. Jesse is going to Vienna to catch a flight back to the United States, whereas Celine is returning to university in Paris after visiting her grandmother. When they reach Vienna, Jesse convinces Celine to disembark with him, saying that 10 or 20 years down the road, she might not be happy with her marriage and might wonder how her life would have been different if she had picked another guy, and this is a chance to realize that he himself is not that different from the rest; in his words, he is "the same boring, unmotivated guy." Jesse has to catch a flight early in the morning and does not have enough money to rent a room for the night, so they decide to roam around in Vienna. After visiting a few landmarks in Vienna, they share a kiss at the top of the Wiener Riesenrad at sunset and start to feel a romantic connection. As they continue to roam around the city, they begin to talk more openly with each other, with conversations ranging from topics about love, life, religion, and their observations of the city. Celine tells Jesse that her last boyfriend broke up with her six months ago, claiming that she "loved him too much". When questioned, Jesse reveals he had initially come to Europe to spend time with his girlfriend who was studying in Madrid, but they had broken up when she was avoiding him while he was there. He decided to take a cheap flight home, via Vienna, but it did not leave for two weeks so he bought a Eurail pass and traveled around Europe. When they are walking alongside the Donaukanal (Danube canal) they are approached by a man who, instead of begging, offers to write them a poem with a word of their choice in it. Jesse and Celine decide on the word "milkshake", and are soon presented with the poem Delusion Angel (written for the film by the poet David Jewell). In a traditional Viennese cafe, Jesse and Celine stage fake phone conversations with each other, playing each other's friends they pretend to call. Celine reveals that she was ready to get off the train with Jesse before he convinced her. Jesse reveals that after he broke up with his girlfriend, he bought a flight that really was not much cheaper, and all he really wanted was an escape from his life. They admit their attraction to each other and how the night has made them feel, though they understand that they probably will not see each other again when they leave. They simply decide to make the best of what time they have left, ending the night with the implication of a sexual encounter between them. At that point, Jesse explains that if given the choice, he would marry her instead of never seeing her again. The film ends the next day at the train station, where, just as Celine's train is about to leave, the couple decides not to exchange any contact information but instead to meet at the same place in six months.

A: Strangers on a train, Jesse convinces Cline to disembark with him in what city?
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Q: Dot1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, Dot1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3. We have solved a 2.5 A resolution structure of the catalytic domain of human Dot1, hDOT1L, in complex with S-adenosyl-L-methionine (SAM). The structure reveals a unique organization of a mainly alpha-helical N-terminal domain and a central open alpha/beta structure, an active site consisting of a SAM binding pocket, and a potential lysine binding channel. We also show that a flexible, positively charged region at the C terminus of the catalytic domain is critical for nucleosome binding and enzymatic activity. These structural and biochemical analyses, combined with molecular modeling, provide mechanistic insights into the catalytic mechanism and nucleosomal specificity of Dot1 proteins.

A:
What is the characteristic domain of histone methyltransferases?
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