Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

Empagliflozin is an orally available, potent and highly selective inhibitor of the sodium glucose cotransporter 2 (SGLT2). This study was undertaken to investigate the effect of food on the pharmacokinetics of 25 mg empagliflozin and to assess dose proportionality between 10 mg and 25 mg empagliflozin under fasted conditions. In this open-label, 3-way, cross-over study, 18 healthy volunteers received 3 single doses of empagliflozin in a randomized sequence (25 mg empagliflozin under fasted conditions, 25 mg empagliflozin after a high-fat, high-calorie breakfast and 10 mg empagliflozin under fasted conditions), each separated by a washout period of at least 7 days. Serial plasma samples were collected at selected time points over a period of 72 hours. Administration with food had no clinically relevant effect on the area under the plasma concentration-time curve (AUC0-) of empagliflozin (geometric mean ratio (GMR): 84.04, 90% confidence interval (CI): 80.86 - 87.34). The decrease observed in the maximum plasma concentrations (Cmax) of empagliflozin (GMR: 63.22, 90% CI: 56.74 - 70.44) when administered with food was not considered clinically meaningful. The increases in AUC0- and Cmax for 10 mg vs. 25 mg empagliflozin administered under fasting conditions were roughly dose-proportional, as demonstrated by the slope of the regression lines being slightly less than 1 (slope for AUC0-: 0.94, 95% CI: 0.90 - 0.97; slope for Cmax: 0.91, 95% CI: 0.80 - 1.01). Empagliflozin was well tolerated under fed and fasting conditions. The results support administration of empagliflozin tablets independently of food. Increases in empagliflozin exposure under fasting conditions were roughly dose-proportional between 10 mg and 25 mg empagliflozin.
Which protein does empagliflozin inhibit?

To study the mechanism of centrosome duplication in cycling cells, we established a novel system of multiple centrosome formation in two types of cells: CHO cells treated with RO3306, a Cyclin-dependent kinase 1 (Cdk1) inhibitor and DT40 cells, in which Cdks were knocked out by chemical genetics. Cdk1-inactivated cells initiated DNA replication and centrosome duplication at the onset of S phase. They became arrested at the end of G2, but the centrosome cycle continued to produce supernumerary centrioles/centrosomes without DNA endoreplication in those cells. Centrosomes were amplified in a highly synchronous and reproducible manner: all of them were located next to the nucleus and spread widely apart from each other with several m in distance. Double knockout of Cdk1 and Cdk2 caused cell cycle arrest at G1/S and centrosomes were no longer duplicated. However, cells continued to grow and increased their volume over 10-fold during 48 hr of culture. Centrosome components, including -tubulin and Cep135, were synthesized and accumulated during the arrest, allowing rapid centrosome multiplication upon recovery from the cell cycle arrest or expression of exogenous Plk4 in G1/S cells. Thus centrosome amplification results from the discoordination of the centrosome cycle from the progression of other cell cycle events, which is controlled by different levels of Cdk activities.
Where in the cell do we find the protein Cep135?

Upon receiving a divorce in Reno, Helen Trent (Claire Trevor) plans to return to San Francisco and goes back to the boarding house where she is renting a room from Mrs. Kraft (Esther Howard) but discovers two dead bodies in the kitchen. Sam Wild (Lawrence Tierney) is the murderer and has committed the crime out of a jealous drive - one of the bodies was his girl friend Laury Palmer (Isabel Jewell) and the other a new man she was dating. Helen grabs her bags and heads for the train and coincidentally Sam does too pushed by his close friend Marty Waterman (Elisha Cook, Jr.) who stays in Reno to get details on the murder and agrees to meet Sam later. Coincidentally Sam and Helen meet on the train and are attracted to each other in part because in a casino earlier they had flirted across the Craps table. In San Francisco Helen parts with Sam but he crashes in on her and her fiancee Fred Grover (Phillip Terry) and her half-sister Georgia Staples (Audrey Long) who has inherited a large newspaper company. Sam sees a good thing in Grace and successfully seduces her into marrying him partly for the power her money can bring him but also to sustain the attraction he has for Helen. Mrs. Kraft hires seedy private detective Matthew Arnett (Walter Slezak) to find out who killed her good friend Laury and the shamus arrives on the wedding day of Sam and Georgia. Helen manages to leak some information to Arnett but Sam discovers this as well and gets his buddy Marty to track down Mrs. Kraft to put an end to things. But this plan goes awry when Marty stops to try to get more information from Helen and Sam perceives it as a play for the woman. Sam reacts in a jealous pique and Helen realizes that he must be stopped and calls Arnett to bring the police with the information he has gathered from his investigation. Sam discovers Helen's communication with Arnett and pulls his gun just as the police arrive resulting in a climactic shootout.

What is Georgia's relationship to Helen?