In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Ex Input:
Campylobacter jejuni remains one of the major gut pathogens of our time. Its zoonotic nature and wide-spread distribution in industrialized countries calls for a quick and reliable diagnostic tool. Antibody-based detection presents a suitable means to identify pathogenic bacteria. However, the knowledge about immunodominant targets is limited. Thus, an approach is presented, which allows for the rapid screening of numerous cDNA derived expression clones to identify novel antigens. The deeper understanding of immunodominant proteins assists in the design of diagnostic tools and furthers the insight into the bacterium's pathogenicity as well as revealing potential candidates for vaccination. We have successfully screened 1536 clones of an expression library to identify 22 proteins that have not been described as immunodominant before. After subcloning the corresponding 22 genes and expression of full-length proteins, we investigated the immunodominant character by microarrays and ELISA. Subsequently, seven proteins were selected for epitope mapping. For cj0669 and cj0920c linear epitopes were identified. For cj0669, specificity assays revealed a specific linear epitope site. Consequently, an eleven amino acid residue sequence TLIKELKRLGI was analyzed via alanine scan, which revealed the glycine residue to be significant for binding of the antibody. The innovative approach presented herein of generating cDNAs of prokaryotes in combination with a microarray platform rendering time-consuming purification steps obsolete has helped to illuminate novel immunodominant proteins of C.jejuni. The findings of a specific linear epitope pave the way for a plethora of future research and the potential use in diagnostic applications such as serological screenings. Moreover, the current approach is easily adaptable to other highly relevant bacteria making it a formidable tool for the future discovery of antigens and potential biomarkers. Consequently, it is desirable to simplify the identification of structural epitopes, as this would extend the spectrum of novel epitopes to be detected.

Ex Output:
Rapid Identification of Novel Immunodominant Proteins and Characterization of a Specific Linear Epitope of Campylobacter jejuni


Ex Input:
Bacterial and viral infections occur early and recurrently in life and thereby impose a substantial disease burden. Besides causing clinical symptoms, a potential role of infection in the development of the asthma syndrome later in life has also been suggested. However, whether bacterial and viral infections unmask host factors in children at risk of asthma or whether they directly cause asthma remains unclear; both viewpoints could be justifi ed, but the underlying mechanisms are complex and poorly understood. Recently, the role of the bacterial microbiome has been emphasised. But data are still sparse and future studies are needed for defi nitive conclusions to be made. In this Review, we discuss present knowledge of viruses and bacteria that infect and colonise the respiratory tract and mucosal surfaces, including their timepoint of action, host factors related to infection, and their eff ect on childhood asthma. Childhood asthma could be the result of a combination of altered host susceptibility and infectious agents.

Ex Output:
Prenatal and childhood infections: implications for the development and treatment of childhood asthma


Ex Input:
Expected risk of infection from patients to healthcare workers in an AIIR was found. • We investigated how to remove airborne contamination effectively in AIIRs. • Both full-scale field experiments and CFD simulations were performed. • Transport of gaseous pollutants is indicated by tracer gas. • By changing the exhaust air flow, the exposure pollutant level was significantly reduced. This study, that is practice-based learning in a real hospital construction project, has evaluated the ventilation performance of three strategies in the protection of health care workers and HVAC control for airborne infectious diseases induced by contaminated exhaled air from patients in a negative pressure isolation room. This paper examines air flow path and airborne pollutant distribution by computational fluid dynamics modeling and field measurement. In hospitals, the risk of virus diffusion mainly depends on air flow behavior and changes in direction caused by supply air and exhaust air locations. An improved isolation room ventilation strategy has been suggested, and is found to be the most efficient in removing contaminants based on the observations and simulation results from three ventilation systems. The results show that ventilation systems utilizing the "lowlevel extraction" technique are very effective at removing pollutants in the human breathing zone. A new clean isolation room ventilation strategy has been developed that employs two exhaust air grilles on the wall behind the bed at low floor level, coupled with a fan filter unit, and is found to have the highest pollutant removal efficiency.

Ex Output:

Investigation on the contaminant distribution with improved ventilation system in hospital isolation rooms: Effect of supply and exhaust air diffuser configurations