In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Q: The Tasmanian devil (Sarcophilus harrisii), so named for its blood-curdling, nocturnal shrieks and snarls, is the largest of the carnivorous marsupials. Although once widely persecuted, concerted efforts are now being made to save the devil from extinction following the emergence of a fatal transmissible malignancy known as devil facial tumour disease (DFTD). DFTD is unusual in that the infectious agent is the cancer cell itself. This chapter discusses the aetiology and pathogenesis of DFTD as well as the profound impact the spread of DFTD has had on the devil's conservation status. Strategies for managing DFTD and conserving the devil will be explored and the contribution of new sequencing technology to the field of conservation genetics and genomics will be examined with regard to the Tasmanian devil and DFTD.

A: Devil Facial Tumour Disease (DFTD): Using Genetics and Genomics to Investigate Infectious Disease in an Endangered Marsupial
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Q: The sudden death of three calves, one diarrheic calf, and one aborted fetus from four farms in southern Brazil was investigated. Two Histophilus somni-associated syndromes were identified: systemic histophilosis (n=4) and abortion (n=1). The principal pathological findings included vasculitis, meningoencephalitis with thrombosis, necrotizing myocarditis, renal infarctions, hepatic abscesses, and bronchopneumonia. PCR assays were used to amplify specific amplicons of the ovine herpesvirus 2, bovine herpesvirus 1 and −5, Listeria monocytogenes, H. somni, and pestivirus; bovine group A rotavirus (BoRV-A) and bovine coronavirus (BCoV) were investigated in calves with diarrhea. H. somni DNA was amplified in tissues from all calves and the brain of the aborted fetus with pathological alterations consistent with histophilosis. All other PCR assays were negative; BoRV-A and BCoV were not identified. These findings confirm the participation of H. somni in the pathological alterations observed in this study and represent the first description of histophilosis in cattle from Brazil.

A: Histophilus somni-induced infections in cattle from southern Brazil
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Q: Members of the family Coronaviridae have the largest genomes of all RNA viruses, typically in the region of 30 kilobases. Several coronaviruses, such as Severe acute respiratory syndrome-related coronavirus (SARS-CoV) and Middle East respiratory syndrome-related coronavirus (MERS-CoV), are of medical importance, with high mortality rates and, in the case of SARS-CoV, significant pandemic potential. Other coronaviruses, such as Porcine epidemic diarrhea virus and Avian coronavirus, are important livestock pathogens. Ribosome profiling is a technique which exploits the capacity of the translating ribosome to protect around 30 nucleotides of mRNA from ribonuclease digestion. Ribosome-protected mRNA fragments are purified, subjected to deep sequencing and mapped back to the transcriptome to give a global "snap-shot" of translation. Parallel RNA sequencing allows normalization by transcript abundance. Here we apply ribosome profiling to cells infected with Murine coronavirus, mouse hepatitis virus, strain A59 (MHV-A59), a model coronavirus in the same genus as SARS-CoV and MERS-CoV. The data obtained allowed us to study the kinetics of virus transcription and translation with exquisite precision. We studied the timecourse of positive and negative-sense genomic and subgenomic viral RNA production and the relative translation efficiencies of the different virus ORFs. Virus mRNAs were not found to be translated more efficiently than host mRNAs; rather, virus translation dominates host translation at later time points due to high levels of virus transcripts. Triplet phasing of the profiling data allowed precise determination of translated reading frames and revealed several translated short open reading frames upstream of, or embedded within, known virus protein-coding regions. Ribosome pause sites were identified in the virus replicase polyprotein pp1a ORF and investigated experimentally. Contrary to expectations, ribosomes were not found to pause at the ribosomal frameshift site. To our knowledge this is the first application of ribosome profiling to an RNA virus. Ribosome profiling is emerging as a powerful technique to monitor translation in living cells at sub-codon resolution. It has particular applicability to virology, with the capacity to identify viral mRNAs that are being translated during infection and to provide new insights into virus gene expression, regulation and host-virus interactions. In this work, we carried out the first ribosome profiling analysis of an RNA virus, using as a model system the murine coronavirus strain MHV-A59, a betacoronavirus in the same genus as the medically important SARS-CoV and MERS-CoV. Parallel ribosome profiling and RNA sequencing of infected-cell time points was performed during the course of MHV replication in mouse tissue culture cells and used to determine virus gene expression kinetics and the relative translational efficiencies of virus and host mRNAs. The sensitivity and precision of the approach permitted us to uncover several unanticipated features of coronavirus translation, giving insights into ribosomal frameshifting, ribosome pausing, and the utilisation of short, potentially regulatory, upstream open reading frames. We also identified some challenges associated with the technique that are of general relevance to the ribosome profiling technique and developed bioinformatic strategies to address these. complement) during synthesis of subgenomic negative-sense templates that then give rise to the positive-sense mRNAs. The process of discontinuous transcription during negative-strand RNA synthesis takes place via polymerase "jumping" at specific "transcription regulatory sequences" (TRSs) on the gRNA template. In MHV, mRNAs 2 to 7 encode, respectively, proteins 2 and haemagglutinin-esterase (HE), spike (S), protein 4, protein 5 and envelope (E), membrane (M), nucleocapsid (N) and internal protein (I), with mRNAs 5 and 7 being functionally bicistronic (Fig 1A) [6]. In the laboratory-adapted strain (MHV-A59) employed in the present study, however, expression of HE and protein 4 is defective. Fig 1. MHV RNA synthesis and translation. (A) Transcript map of the 31335-nt MHV-A59 genome. Polyproteins pp1a and pp1b are translated from the genomic RNA, with pp1b being expressed as a transframe fusion with pp1a (i.e. pp1ab) via −1 programmed ribosomal frameshifting (−1 PRF). The 3 0 ORFs are expressed from a series of subgenomic RNAs produced during infection. Each subgenomic RNA contains a 5 0 leader sequence that is identical to the 5 0 leader of the genome, appended via polymerase "jumping" between body transcription regulatory sequences (TRSs) (green diamonds) and the leader TRS (orange diamond) during negative-strand synthesis. Due to mutations present in this laboratory-adapted strain, the hemagglutinin-esterase and ORF4 gene fragments (HE and 4; grey boxes) are not expected to be translated. (B) RiboSeq CHX (red) and RNASeq (green) densities at 5 h p.i. (repeat 1) in reads per million mapped reads (RPM). Read densities are plotted on a log(1+x) scale to cover the wide range in expression across the genome. Histograms show the positions of the 5 0 ends of reads with a +12 nt offset to map (for RPFs) approximate P-site positions. Negative-sense reads are shown in dark blue below the horizontal axis. (C) The positive-sense RiboSeq/RNASeq ratio, after first applying a 15-nt running mean (RM) filter to each individual distribution.

A:
High-Resolution Analysis of Coronavirus Gene Expression by RNA Sequencing and Ribosome Profiling
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