Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

[Q]: Singapore did not have a native population in the true sense of the word as the population became dominated numerically by three main groups of immigrants after the British founded Singapore as a free port in the 19th century. It was estimated that when Raffles arrived in Singapore in January 1819, Singapore had about 120 Malays, 30 Chinese and some local tribes (the Orang Laut) under the rule of the Temenggung. Around 100 of these Malays had originally moved to Singapore from Johor in 1811 led by the Temenggung. Another estimate put the total population of the whole of Singapore at 1,000, mostly of various local tribes. Early census figures of Singapore show a long period of influx of migrant workers into the country, initially mostly Malays, quickly followed by the Chinese. By 1821, the population was estimated to have increased to 4,724 Malays and 1,150 Chinese. In the first census of 1824, 6,505 out of the 10,683 total were Malays and Bugis, constituting over 60% of the population. Large number of Chinese migrants started to enter Singapore just months after it became a British settlement, and they were predominantly male. In 1826, official census figures give a total population of 13,750, with 6,088 Chinese, 4,790 Malays, 1,242 Bugis, 1,021 Indians from Bengal (244) and the Coromandel Coast (777), smaller number of Javanese people (267), Europeans (87) and other peoples. The population total of Singapore increased to 16,000 in 1829, 26,000 five years later. By 1836, the Chinese at 13,749 had become the most populous ethnic group, overtaking the broad Malay grouping (12,538, including other groups such as the Bugis, Javanese, and Balinese). By the beginning of 1850, the population had reached 60,000, 24,790 of them Chinese.
[A]: What was the nationality of Raffles?


[Q]: The Bacillus anthracis toxin genes, cya, lef, and pag, can be viewed as a regulon, in which transcription of all three genes is activated in trans by the same regulatory gene, atxA, in response to the same signal, CO2. In atxA+ strains, toxin gene expression is increased 5- to 20-fold in cells grown in 5% CO2 relative to cells grown in air. CO2-enhanced toxin gene transcription is not observed in atx4-null mutants. Here, we used two independent techniques to obtain evidence for additional CO2-induced atxA-regulated genes. First, total protein preparations from atxA4+ and atxA isolates grown in 5% CO2 and in air were examined by two-dimensional electrophoresis. Comparison of the resulting protein patterns indicated that synthesis of non-toxin proteins is influenced by growth in elevated CO2 and the toxin gene regulator, atxA. Second, we generated random transcriptional lacZ fusions in B. anthracis with transposon Tn917-LTV3. Transposon-insertion libraries were screened for mutants expressing CO2-enhanced atxA-dependent beta-galactosidase activity. DNA sequence analysis of transposon insertion sites in 17 mutants carrying CO2- and atxA-regulated fusions revealed 10 mutants carrying independent insertions on the 185-kb toxin plasmid pXO1 which did not map to the toxin genes. The tcr-lacZ fusion mutants (tcr for toxin coregulated) were Tox+, indicating that these genes may not be involved in anthrax toxin gene activation. Our data indicate a clear association of atxA with CO2-enhanced gene expression in B. anthracis and provide evidence that atxA regulates genes other than the structural genes for the anthrax toxin proteins.
[A]: Which metabolite activates AtxA?


[Q]: Earthquake magnitude affects how much damage is done in an earthquake. A larger earthquake damages more buildings and kills more people than a smaller earthquake. But thats not the only factor that determines earthquake damage. The location of an earthquake relative to a large city is important. More damage is done if the ground shakes for a long time. The amount of damage also depends on the geology of the region. Strong, solid bedrock shakes less than soft or wet soils. Wet soils liquefy during an earthquake and become like quicksand. Soil on a hillside that is shaken loose can become a landslide. Hazard maps help city planners choose the best locations for buildings (Figure 7.38). For example, when faced with two possible locations for a new hospital, planners must build on bedrock rather than silt and clay. The 1985 Mexico City earthquake measured magnitude 8.1. The earthquake killed at least 9,000 people, injured 30,000 more, and left 100,000 people homeless. It destroyed 416 buildings, and seriously damaged 3,000 other buildings. The intense destruction was due to the soft ground the city is built on. Silt and clay fill a basin made of solid rock. In an earthquake, seismic waves bounce back-and-forth off the sides and bottom of the rock basin. This amplifies the shaking. The wet clay converts to quicksand (Figure 7.39). Many buildings were not anchored to bedrock. They settled into the muck. This caused enormous damage. Water, sewer, and electrical systems were destroyed, resulting in fires. Acapulco was much closer to the epicenter, but since the city is built on bedrock it suffered little damage. The amount of damage depends on the amount of development in the region. The 1964 Great Alaska Earthquake, near Anchorage, was the largest earthquake ever recorded in North America. The gigantic quake had a magnitude of 9.2. The earthquake lasted for several minutes and the ground slipped up to 11.5 meters (38 feet). An area of 100,000 square miles (250,000 square km) was affected. The ground liquefied, causing landslides (Figure 7.40). The earthquake occurred at a subduction zone, and large tsunami up to 70 meters (20 feet) high were created. Despite the intensity of the earthquake, only 131 people died. Most deaths were due to the tsunami. Property damage was just over $300 million ($1.8 billion in 2007 U.S. dollars). The reason there was such a small amount of damage is that very few people lived in the area (Alaska had only been a state for five years!). A similar earthquake today would affect many more people. Buildings must be specially built to withstand earthquakes. Skyscrapers and other large structures built on soft ground must be anchored to bedrock. Sometimes that bedrock is hundreds of meters below the ground surface! Building materials need to be both strong and flexible. Small structures, like houses, should bend and sway. Wood and steel bend. Brick, stone, and adobe are brittle and will break. Larger buildings must sway, but not so much that they touch nearby buildings. Counterweights and diagonal steel beams can hold down sway. Buildings need strong, flexible connections where the walls meet the foundation. Earthquake-safe buildings are well connected (Figure Steel or wood can be added to older buildings to reinforce a buildings structure and its connections (Figure 7.42). Elevated freeways and bridges can also be reinforced so that they do not collapse. Important structures must be designed to survive intact. One of the biggest problems caused by earthquakes is fire. Fires start because earthquakes rupture gas and electrical lines. Water mains may break. This makes it difficult to fight the fires. The shapes of pipes can make a big difference. Straight pipes will break in a quake. Zigzag pipes bend and flex when the ground shakes. In San Francisco, water and gas pipelines are separated by valves. Areas can be isolated if one segment breaks. Strong, sturdy structures are expensive to build. Communities must decide how safe to make their buildings. They must weigh how great the hazard is, what different building strategies will cost, and how much risk they are
[A]:
Not too many people died in the Great Alaska Earthquake in 1964 because