In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Q: Background: Surveillance is essential to estimating the global burden of pneumonia, yet differences in surveillance methodology and health care-seeking behaviors limit inter-country comparisons. Methods: Results were compared from community surveys measuring health care-seeking for pneumonia defined as: (1) cough and difficulty breathing for P2 days; or, (2) provider-diagnosed pneumonia. Surveys were conducted in six sites in Guatemala, Kenya and Thailand; these sites also conduct, active, hospital-and population-based disease surveillance for pneumonia. Results: Frequency of self-reported pneumonia during the preceding year ranged from 1.1% (Thailand) to 6.3% (Guatemala) and was highest in children aged <5 years and in urban sites. The proportion of persons with pneumonia who sought hospitalbased medical services ranged from 12% (Guatemala, Kenya) to 80% (Thailand) and was highest in children <5 years of age. Hospitals and private provider offices were the most common places where persons with pneumonia sought health care. The most commonly cited reasons for not seeking health care were: (a) mild illness; (b) already recovering; and (3) cost of treatment. Conclusions: Health care-seeking patterns varied widely across countries. Using results from standardized health care utilization surveys to adjust facility-based surveillance estimates of pneumonia allows for more accurate and comparable estimates.

A: Putting surveillance data into context: The role of health care utilization surveys in understanding population burden of pneumonia in developing countries
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Q: strains of infectious bronchitis virus (IBV) were isolated from the vaccinated chicken flocks in Sichuan province, China. The S1 genes of the isolates were amplified and sequenced. Phylogenetic analysis revealed that the 19 isolates and 37 reference IBV strains can be grouped into eight genotypes. Although IBVs of Taiwan-I type, massachusetts type, and proventriculitis type were isolated, but most isolates were LX4 genotype. Homology analysis of the sequences of S1 genes of the 19 isolates and 37 reference IBV strains revealed that the identity of the nucleotides and amino acid sequences of the S1 genes between the 15 LX4-type isolates and other IBV strains were 71.9-99.3% and 72.1-99.1%, respectively, while those of the analyzed IBV of LX4 type were 96.0-99.9% and 94.3-99.8%, respectively. The results from this study and other published results in the GenBank database showed that isolates circulating in Sichuan province in recent years were mainly LX4 genotype, which is the predominant genotype circulated in China in recent years.

A: Genetic analysis revealed LX4 genotype strains of avian infectious bronchitis virus became predominant in recent years in Sichuan area, China
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Q: Delivery of native or chemically modified recombinant proteins into mammalian cells shows 31 promise for functional investigations and various technological applications, but concerns 32 that sub-cellular localization and functional integrity of delivered proteins may be affected 33 remain high. Here, we surveyed batch electroporation as a delivery tool for single 34 polypeptides and multi-subunit protein assemblies of kinetochores, a spatially confined and 35 well-studied subcellular structures. After electroporation in human cells, recombinant 36 fluorescent Ndc80 and Mis12 multi-subunit complexes displayed native localization, 37 physically interacted with endogenous binding partners, and functionally complemented 38 depleted endogenous counterparts to promote mitotic checkpoint signaling and 39 chromosome segregation. Farnesylation is required for kinetochore localization of the 40 Dynein adaptor Spindly. In cells with chronically inhibited farnesyl transferase activity, in 41 vitro farnesylation and electroporation reconstituted robust kinetochore localization of 42 Spindly. Thus, electroporation is uniquely versatile for delivering synthetic and, as required, 43 chemically modified functional mimics of endogenous proteins, and is therefore a promising 44 tool for chemical and synthetic biology.

A:
Electroporated recombinant proteins as tools for in vivo functional
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