In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

[Q]: Combinations of porcine respiratory coronavirus (PRCV) and either of two swine influenza viruses (H1N1 or H3N2) were administered intranasally and by aerosol to six-to eight-week-old specific pathogen:free pigs. The clinical responses, gross respiratory lesions and growth performances of these pigs were studied and compared with those of single (PRCV, H1N1 or H3N2) and mock-infected animals. PRCV infection caused fever, growth retardation and lung lesions, but no respiratory symptoms. Infection with swine influenza viruses caused rather similar, mild symptoms of disease, with H1N1 infection being the least severe. Combined infections with influenza viruses and PRCV did not appear to enhance the pathogenicity of these viruses. Furthermore, viruses were isolated more frequently from tissues and nasal swabs taken from 'single' than 'dual' infected animals, suggesting a possible in vivo interference between replication of PRCV and swine influenza virus.
[A]: Pathogenicity of concurrent infection of pigs with porcine respiratory coronavirus and swine influenza virus


[Q]: Background: The novel corona virus SARS-CoV-2 (COVID-19) can infect healthcare workers. We developed an institutional algorithm to protect operating room team members during the COVID-19 pandemic and rationally conserve personal protective equipment (PPE). Study Design: An interventional platform (operating room, interventional suites, and endoscopy) PPE taskforce was convened by the hospital and medical school leadership and tasked with developing a common algorithm for PPE use, to be used throughout the interventional platform. In conjunction with our infection disease experts, we developed our guidelines based upon potential patterns of spread, risk of exposure and conservation of PPE. A decision tree algorithm describing our institutional guidelines for precautions for operating room team members was created. This algorithm is based on urgency of operation, anticipated viral burden at the surgical site, opportunity for a procedure to aerosolize virus, and likelihood a patient could be infected based on symptoms and testing. Conclusion: Despite COVID-19 being a new threat, we have shown that by developing an easyto-follow decision tree algorithm for the interventional platform teams, we can ensure optimal healthcare worker safety.
[A]: Journal Pre-proof Precautions for Operating Room Team Members during the COVID-19 Pandemic Precautions for Operating Room Team Members during the COVID-19 Pandemic


[Q]: SJG), and John.Skehel@crick.ac.uk (JJS) SUPPLEMENTAL EXPERIMENTAL PROCEDURES: Monoclonal antibody isolation was perfomed as previously described (Pappas et al., 2014; Traggiai et al., 2004) . In brief, peripheral blood mononuclear cell (PBMC) samples were obtained from a single healthy donor after vaccination following written consent. Memory B cells were isolated from cryopreserved or fresh PBMCs using CD22 microbeads or anti-FITC (fluorescein isothiocyanate) microbeads (Miltenyi Biotec) after staining of PBMCs with CD22-FITC, and were immortalized with Epstein-Barr virus (EBV) and CpG in multiple wells. Supernatants of immortalized B cell clones containing monoclonal antibodies were screened for binding to HA proteins representing two different HA subtypes. Using this methodology, we identified a monoclonal from primary screening in ELISA assay that showed crossreactive binding to recombinant HA proteins of A/Vietnam/2005 H5N1 and A/ Netherlands/2003 H7N7. Variable gene sequences were isolated from this cross-reactive clone by RT-PCR, cloned and expressed as a full-length IgG transiently in 293 T cells. The lead FY1 antibody was further modified to revert the non-germline framework amino acid changes and affinity optimize the CDRs. For the affinity optimization, full-length IgG constructs containing the different variants created by CDR based parsimonious mutagenesis were expressed transiently in 293 T cells and supernatants were screened by ELISA to select clones that resulted in increased binding activity to H3 while maintaining or increasing the binding activity to H1 HA proteins. Coating concentrations of 0.15 µg/ml of rabbit anti-human IgG were used to capture and normalize variant IgG constructs from supernatants, and binding was probed using 0.5 µg/ml of biotinylated HA proteins A/Perth/2009 H3N2 and A/California/7/2009 H1N1 followed by the addition of streptavidin-HRP (1:5000). Beneficial single mutations were combined and cloned into a combinatorial library, which were expressed and screened by ELISA as described above, leading to the generation of MEDI8852. The DNA Maximum Likelihood program (Dnaml) of the PHYLIP package, version 3.69, was used to estimate immunoglobulin phylogenies from nucleotide sequences that were first aligned using ClustalW2, as previously described12. IgHCDR3 regions were defined with their Kabat numbering using the software available on the Abnum website (http://www.bioinf.org.uk/abs/abnum/). The V, D and J genes of the IgH DNA sequences were identified using the IMGT database as a reference (Brochet et al., 2008; Ye et al., 2013) . The UCA sequences were inferred with Antigen Receptor Probabilistic Parser (ARPP) UA Inference software (Kepler, 2013) , and produced by gene synthesis (Genscript). Branchpoint antibodies were determined by the antibody phylogenetic tree derived by dnaml, and produced by gene synthesis (Genscript). Recombinant HA proteins were expressed and purified as previously described (Benjamin et al., 2014) ELISA binding assays were performed using 384 well plates (Maxisorp, Nunc) with 0.5 µg/ml of purified HA protein as described in Table S2 . ELISA plates were washed, blocked 1% (w/v) casein (Thermo Scientific), and serially diluted antibodies were incubated for 1 hr at room temperature. Bound antibodies were detected using a peroxidase-conjugated mouse anti-human IgG antibody (KPL 1:10,000), followed by development with TMB solution (KPL). The absorbance at 450nm was measured, and EC 50 values were calculated using a non-linear regression of log (inhibitor) vs response in Graph Pad Prism. Cell surface expressed protein binding assays were conducted using flow cytometry to detect binding of antibodies to HA transfected cells. HEK 293 cells were transiently transfected with full-length wild type HA expressing plasmids. Forty-eight hours after transfection, cells were detached with trypsin, and incubated with 5 µg/ml of FY1 or MEDI8852 on ice for 1 hour. Antibody remaining bound to surface expressed HA protein after washing was then stained with a goat anti-human IgG Daylight 649 (Jackson ImmunoResearch), and detected by flow cytometry.
[A]:
Supplemental Information Structure and Function Analysis of an Antibody Recognizing All Influenza A Subtypes