instruction:
In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".
question:
Paragraph: Background: Embryonated chicken eggs have been used since the mid-20th century to grow a wide range of animal viruses to high titers. However, eggs have found so far only limited use in the production of recombinant proteins. We now describe a system, based on a Sendai virus minigenome, to produce large amounts of heterologous viral glycoproteins in the allantoic cavity of embryonated eggs. Results: Soluble forms of human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV) fusion (F) proteins, devoid of their transmembrane and cytoplasmic domains, were produced in allantoic fluids using the Sendai minigenome system. The first step was rescuing in cell cultures Sendai virus minigenomes encoding the proteins of interest, with the help of wild type Sendai virus. The second step was propagating such recombinant defective viruses, together with the helper virus, in the allantoic cavity of chicken embryonated eggs, and passage to optimize protein production. When compared with the production of the same proteins in the culture supernatant of cells infected with vaccinia recombinants, the yield in the allantoic fluid was 5-10 fold higher. Mutant forms of these soluble proteins were easily constructed by site-directed mutagenesis and expressed in eggs using the same approach. The simplicity and economy of the Sendai minigenome system, together with the high yield achieved in the allantoic fluid of eggs, makes it an attractive method to express soluble glycoproteins aimed for structural studies. 
 Title: High level expression of soluble glycoproteins in the allantoic fluid of embryonated chicken eggs using a Sendai virus minigenome system
answer:
True


question:
Paragraph: Clinical metagenomics (CMg) is the discipline that refers to the sequencing of all nucleic acid material present within a clinical specimen with the intent to recover clinically relevant microbial information. From a diagnostic perspective, next-generation sequencing (NGS) offers the ability to rapidly identify putative pathogens and predict their antimicrobial resistance profiles to optimize targeted treatment regimens. Since the introduction of metagenomics nearly a decade ago, numerous reports have described successful applications in an increasing variety of biological specimens, such as respiratory secretions, cerebrospinal fluid, stool, blood and tissue. Considerable advancements in sequencing and computational technologies in recent years have made CMg a promising tool in clinical microbiology laboratories. Moreover, costs per sample and turnaround time from specimen receipt to clinical management continue to decrease, making the prospect of CMg more feasible. Many difficulties, however, are associated with CMg and warrant further improvements such as the informatics infrastructure and analytical pipelines. Thus, the current review focuses on comprehensively assessing applications of CMg for diagnostic and subtyping purposes. Crown 
 Title: Highlighting Clinical Metagenomics for Enhanced Diagnostic Decision-making: A Step Towards Wider Implementation
answer:
True


question:
Paragraph: Transmissible gastroenteritis virus (TGEV) is a coronavirus that causes villus atrophy, followed by crypt hyperplasia, reduces the activities of intestinal digestive enzymes, and disrupts the absorption of intestinal nutrients. In vivo, TGEV primarily targets and infects intestinal epithelial cells, which play an important role in glucose absorption via the apical and basolateral transporters Na + -dependent glucose transporter 1 (SGLT1) and facilitative glucose transporter 2 (GLUT2), respectively. In this study, we therefore sought to evaluate the effects of TGEV infection on glucose uptake and SGLT1 and GLUT2 expression. Our data demonstrate that infection with TGEV resulted in increased glucose uptake and augmented expression of EGFR, SGLT1 and GLUT2. Moreover, inhibition studies showed that EGFR modulated glucose uptake in control and TGEV infected cells. Finally, high glucose absorption was subsequently found to promote TGEV replication. Competing Interests: The authors have declared that no competing interests exist. EGFR and SGLT1 protein was dynamic. (A) Cells were treated with PBS, DMSO (100 nM), or AG1478 (100 nM) at 37˚C for 48 h. The culture medium from each group was then assayed. (B) Western blot analysis of the expression levels of p-EGFR, EGFR, SGLT1, GLUT2, and β-tubulin. Experiments were performed separately three times. (C) The intensities of protein bands were quantified using Quantity One and SPSS software. (D) Cells were transfected with shEGFR, shEGFR control, pLVX-EGFR, or pLVX-EGFR control. After 48 h of incubation, culture medium from each group and from mock-infected cells was assayed. (E) The expression levels of p-EGFR, EGFR, SGLT1, GLUT2, and β-tubulin proteins were evaluated by western blot analysis. Experiments were performed separately three times. (F) The intensities of protein bands were quantified using Quantity One and SPSS software. (G) IPEC-J2 cells were treated with phlorizin (500 μM) at 37˚C for 48 h, and culture medium from each group was assayed. (H) The expression levels of SGLT1, EGFR, and β-tubulin were evaluated by western blot analysis. (I) The intensities of protein bands were quantified using Quantity One and SPSS software. (J) IPEC-J2 cells were treated with three SGLT1-specific siRNA molecules for 48 h, and culture medium from each group was assayed. (K) Western blot analysis of the expression levels of SGLT1, EGFR, and β-tubulin. (L) The intensities of protein bands were quantified using Quantity One and SPSS software. Each experiment was performed separately three times. 
 Title: Transmissible Gastroenteritis Virus Infection Enhances SGLT1 and GLUT2 Expression to Increase Glucose Uptake
answer:

False