In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

[Q]: We investigated the ability of monoclonal B cells to restore primary and secondary T-cell dependent antibody responses in adoptive immune-deficient hosts. Priming induced B cell activation and expansion, AID expression, antibody production and the generation of IgM + IgGand IgM -IgG + antigen-experienced B-cell subsets that persisted in the lymphopenic environment by cell division. Upon secondary transfer and recall the IgM -IgG + cells responded by the production of antigen-specific IgG while the IgM + memory cells secreted mainly IgM and little IgG, but generated new B cells expressing germinal center markers. The recall responses were more efficient if the antigenic boost was delayed suggesting that a period of adaptation is necessary before the transferred cells are able to respond. Overall these findings indicate that reconstitution of a functional and complete memory pool requires transfer of all different antigen-experienced B cell subsets. We also found that the size of the memory B cell pool did not rely on the number of the responding naïve B cells, suggesting autonomous homeostatic controls for naïve and memory B cells. By reconstituting a stable memory B cell pool in immune-deficient hosts using a monoclonal high-affinity B cell population we demonstrate the potential value of B cell adoptive immunotherapy.
[A]: Regulation and Maintenance of an Adoptive T-Cell Dependent Memory B Cell Pool


[Q]: Madin Darby canine kidney (MDCK) cells were adapted to serum-free RPMI 1640 medium and used for cultivation of canine viruses. RPMI 1640 medium was supplemented with a soybean peptone, l-glutamine and antibiotics, so that the protein concentration was less than 5 g/ml (RPMI/SP medium). The resulting adapted MDCK-SP cells showed steady growth after the twenty-eighth passage in RPMI/SP medium (MDCK-SP cell culture). Canine distemper virus, canine parvovirus, canine adenoviruses and canine parainfluenza virus, which are the principal components of canine combined virus vaccines, grew in the MDCK-SP cell culture as efficiently as the parental MDCK cells cultured in the conventional Eagle's MEM containing fetal bovine serum. Consequently, the use of MDCK-SP cell culture can make current canine vaccine products much safer, of higher quality and at lower cost.
[A]: Growth characteristics of canine pathogenic viruses in MDCK cells cultured in RPMI 1640 medium without animal protein


[Q]: Throughout the book chapters, researchers have highlighted the recent advancement in microfluidic areas, particularly those involving microdroplets. Simon and Lee focused on microfluidics droplet manipulations and applications, including droplet fusion, droplet fission, mixing in droplets, and droplet sorting. By combining these operations, they have shown promising applications in executing chemical reactions and biological assays at the microscale. Day and Karimiani discussed dropletisation of bio-reactions. Zhang and Liu elaborated the physics involved in multiphase flows and microdroplets dynamics. They emphasized the important dimensionless parameters relating to droplet dynamics with droplet generation process as an example. Barber and Emerson discussed the fundamental droplet handling operations and the recent advances in electrowetting microdroplet technologies. They also provided an overview of droplet-based electrowetting technologies in biological and chemical applications. P. Neuzil
[A]:
Trends and Perspectives