In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Input: Consider Input: BALB/c male mice were mated with either Swiss-Webster or MF1 females to produce first generation cross-bred offspring. Hybridoma cell lines, from the fusion of P3-NS1-Ag4/1 myeloma cells with spleen cells sensitised to the porcine coronavirus causing transmissible gastroenteritis, were injected intraperitoneally into these mice to produce ascitic fluid containing monoclonal antibodies. Mice of 11 weeks of age weighing between 26 and 34 g were used. The volume of ascites produced by mice injected with four of the five hybrid cell lines tested was greater in the cross-bred offspring than in the BALB/c parent. The fifth cell line gave comparable volumes in the MF1 cross-breed and BALB/c parent but a lesser volume in the Swiss-Webster cross-breed. The antibody titres of the ascites as determined by virus neutralisation, radioimmune and indirect immune fluorescence assays, did not differ significantly between mouse types. The ability to use all offspring from a litter of cross-bred mice, irrespective of sex, and the increased volume of ascitic fluid formed in each mouse, permits fewer animals to be used for the production of ascites in these strains, thereby offering considerable economic and ethical advantages over the use of BALB/c mice.

Output: Comparison of ascites production for monoclonal antibodies in BALB/c and BALB/c-derived cross-bred mice


Input: Consider Input: C57 strain mice were inoculated intraperitoneally with denatured mouse hepatitis virus strain 3 particles and virus surface projection, membrane and ribonucleoprotein subeomponents, obtained from detergent treated purified virus preparations. All immunised animals developed high levels of serum antibody directed against the respective antigens, detectable by enzyme-linked immunosorbent assay. Mice that had been immunised with denatured virus particles or surface projections were protected against infection with mouse hepatitis virus strain 3, whereas immunisation with virus membrane or ribonucleoprotein subcomponents failed to protect mice against virus challenge.

Output: Archives of Virology Antigenieity of Mouse Hepatitis Virus Strain 3 Subeomponents in C57 Strain Miee


Input: Consider Input: We collected serum samples from 50 healthy blood donors (Cohort A) as negative controls Sanquin Blood Bank (Rotterdam, the Netherlands) obtained written informed consent for research use. We assessed specificity using cohorts B and C, which consisted of 145 serum specimens from patients confirmed by RT-PCR to be positive for human respiratory infections. Those included samples from persons recently infected with 13 different respiratory viruses, or with acute IgM-positive CMV and EBV infection, which are known to be cross-reactive in serologic assays; as well as Mycoplasma pneumoniae. Because MERS has no pathognomonic signs distinguishing it from other respiratory infections, cohort B, including 85 samples from acute non-HCoV respiratory infections, was used to assess specificity in acute cases. To further evaluate specificity, cohort C included serum samples from acute to convalescent HCoV-NL63, 229E, and -OC43 patients. Furthermore, we assessed Cohorts D-G were used to evaluate sensitivity of the different platforms. Serologically identified MERS-CoV-infected mild and asymptomatic persons with camel contact (D1) and healthy donors (D2) from Qatar characterized in an earlier study (1) constituted cohort D. Serial serum samples from 2 RT-PCRdiagnosed MERS-CoV case-patients imported to the Netherlands (2) were used as positive controls and to evaluate antibody kinetics; these were 15 serum samples from patient 1, ranging 4-228 days postdiagnosis (dpd), and 13 from patient 2, ranging 1-44 dpd. These 28 samples were categorized into cohorts E (<14 dpd, acute phase) and F (>14 dpd, convalescent phase).


Output: Sensitive and Specific Detection of Low- Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections Serum Samples