This task is about reading the given passage and construct a question about the information present in the passage. Construct a question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) its answer is a continuous text span from the paragraph. Avoid creating questions that (i) can be answered correctly without actually understanding the paragraph and (ii) uses same words or phrases given in the passage.

Q: Serotonin is a neuromodulator involved in regulation of mood and behavior. Development in the limbic system plays an important role in determining rewards and punishments and processing emotional experience and social information. Changes in the levels of the neurotransmitters dopamine and serotonin in the limbic system make adolescents more emotional and more responsive to rewards and stress. The corresponding increase in emotional variability also can increase adolescents' vulnerability. The effect of serotonin is not limited to the limbic system: Several serotonin receptors have their gene expression change dramatically during adolescence, particularly in the human frontal and prefrontal cortex .

A: Which neuromodulator is involved in regulation of mood and behavior?
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Q: Likewise, group theory helps predict the changes in physical properties that occur when a material undergoes a phase transition, for example, from a cubic to a tetrahedral crystalline form. An example is ferroelectric materials, where the change from a paraelectric to a ferroelectric state occurs at the Curie temperature and is related to a change from the high-symmetry paraelectric state to the lower symmetry ferroelectic state, accompanied by a so-called soft phonon mode, a vibrational lattice mode that goes to zero frequency at the transition.

A: What aids in predicting changes of physical traits?
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Q: Zhu Yousong, however, fared a lot worse than his ancestor Zhu Yuanzhang three centuries earlier. Beset by factional conflicts, his regime could not offer effective resistance to Qing forces, when the Qing army, led by the Manchu prince Dodo approached Jiangnan the next spring. Days after Yangzhou fell to the Manchus in late May 1645, the Hongguang Emperor fled Nanjing, and the imperial Ming Palace was looted by local residents. On June 6, Dodo's troops approached Nanjing, and the commander of the city's garrison, Zhao the Earl of Xincheng, promptly surrendered the city to them. The Manchus soon ordered all male residents of the city to shave their heads in the Manchu queue way. They requisitioned a large section of the city for the bannermen's cantonment, and destroyed the former imperial Ming Palace, but otherwise the city was spared the mass murders and destruction that befell Yangzhou.

A:
When did the Hongguang Emperor leave Nanjing, having been defeated?
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