In this task, you are given a sentence from the research paper and your task is to classify the given sentence into the following categories: Background (Why is this problem important? What relevant works have been created before? What is still missing in the previous works? What are the high-level research questions? How might this help other research or researchers?), Purpose (What specific things do the researchers want to do? What specific knowledge do the researchers want to gain? What specific hypothesis do the researchers want to test?), Method (How did the researchers do the work or find what they sought? What are the procedures and steps of the research?), or Finding (What did the researchers find out? Did the proposed methods work? Did the thing behave as the researchers expected?). 

Q: Aminoacylated tRNAs were purified by reversed-phase HPLC on an RP-18 HPLC column ( 250 mm × 10.5 mm , Merck ) equilibrated with buffer ( 20 mM ammonium acetate , pH 5.0 , 10 mM magnesium acetate , 400 mM NaCl ) using a gradient of 0 % -15 % ) ethanol.

A: method
****
Q: Namely , the use of `` omic '' technologies ( next generation sequencing , DNA , and protein microarrays ) for dissecting the underlying host response to infection at the molecular level ; optical imaging techniques ( flow cytometry and fluorescence microscopy ) for assessing cellular responses to infection ; and biophotonic imaging for visualising the infectious disease process.

A: purpose
****
Q: Unexpectedly , the morphology and proliferation rate of iPSC were changed after nCoVN expressing for two weeks.

A:
background
****