Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.
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Question: The 1988 Dungeons & Dragons role-playing game module Ruins of Adventure was produced using the same adventure scenario as Pool of Radiance, using the same plot, background, setting, and many of the same characters as the computer game.

Answer: The Pool of Radiance is based upon what?


Question: Brian Cohen is born in a stable next door to the one in which Jesus is born, which initially confuses the three wise men who come to praise the future King of the Jews. Brian grows up an idealistic young man who resents the continuing Roman occupation of Judea. While attending Jesus' Sermon on the Mount, Brian becomes infatuated with an attractive young rebel, Judith. His desire for her and hatred for the Romans lead him to join the "People's Front of Judea", one of many fractious and bickering independence movements, who spend more time fighting each other than the Romans. After several misadventures, and escaping from Pontius Pilate, Brian winds up in a line-up of would-be mystics and prophets who harangue the passing crowd in a plaza. Forced to come up with something plausible in order to blend in and keep the guards off his back, Brian repeats some of what he had heard Jesus say, and quickly attracts a small but intrigued audience. Once the guards have left, Brian tries to put the episode behind him, but he has unintentionally inspired a movement. He grows frantic when he finds that some people have started to follow him around, with even the slightest unusual occurrence being hailed as a miracle. Each of their responses ever growing in fervor and intensity making it harder and harder for him to get away from them. Yet because of the mob's excitement, they end up leaving Brian alone. After which he sees Judith is one who didn't leave, and they then spend the night together. In the morning, Brian, completely naked, opens the curtains to discover an enormous crowd outside his mother's house who proclaim him to be the Messiah. Brian's mother protests, "He's not the Messiah, he's a very naughty boy."; and, "There's no Messiah in here. There's a mess all right, but no Messiah." Yet all of her attempts at dispersing the crowd are rebuffed. Furthermore, once Brian addresses them, he also finds that he is unable to change their minds. His followers are completely committed to their beliefs in and of Brian's divinity. They immediately seize upon everything he says and does as points of doctrine. The hapless Brian finds no solace as people have even mobbed his mother's house. They fling their afflicted bodies at him demanding miracle cures and divine secrets. After sneaking out the back, Brian is then finally captured and scheduled to be crucified. Meanwhile, a huge crowd has assembled outside the palace. Pilate (together with the visiting Biggus Dickus) tries to quell the feeling of revolution by granting them the choice of one person to be pardoned. The crowd, however, shouts out names containing the letter "r", mocking Pilate's rhotacistic speech impediment. Eventually, Judith appears in the crowd and calls for the release of Brian, which the crowd echoes since the name also contains an "r". Pilate agrees to "welease Bwian". His order is eventually relayed to the guards, but in a scene that parodies the climax of the film Spartacus, various crucified people all claim to be "Brian of Nazareth" and the wrong man is released. Various other opportunities for a reprieve for Brian are denied as, one by one, his "allies" (including Judith and his mother) step forward to explain why they are leaving the "noble freedom fighter" hanging in the hot sun. Hope is renewed when a crack suicide squad from the "Judean People's Front" (not to be confused with the People's Front of Judea) come charging towards the Romans, but rather than fighting to release Brian or the other prisoners, they commit mass suicide as a political protest. Condemned to a long and painful death, Brian finds his spirits lifted by his fellow sufferers, who break into song with "Always Look on the Bright Side of Life."[6]

Answer: Where is Brian Cohen born ?


Question: Allelic polymorphism of the apolipoprotein E (ApoE) gene (ApoE 2, ApoE 3 and ApoE 4 alleles) gives rise to three protein isoforms (ApoE2, ApoE3 and ApoE4) that differ by 1 or 2 amino acids. Inheritance of the ApoE 4 allele is a risk factor for developing Alzheimer's disease (AD). The potential diagnostic value of ApoE protein levels in biological fluids (i.e. cerebrospinal fluid, plasma and serum) for distinguishing between AD patients and healthy elderly subjects is subject to great controversy. Although a recent study reported subnormal total ApoE and ApoE4 levels in the plasma of AD patients, other studies have found normal or even elevated protein levels (versus controls). Because all previously reported assays were based on immunoenzymatic techniques, we decided to develop an orthogonal assay based on targeted mass spectrometry by tracking (i) a proteotypic peptide common to all ApoE isoforms and (ii) a peptide that is specific for the 4 allele. After trypsin digestion, the ApoE4-specific peptide contains an oxidation-prone methionine residue. The endogenous methionine oxidation level was evaluated in a small cohort (n=68) of heterozygous 34 carriers containing both healthy controls and AD patients. As expected, the proportion of oxidized residues varied from 0 to 10%, with an average of 5%. We therefore developed a standardized strategy for the unbiased, absolute quantification of ApoE4, based on performic acid oxidization of methionine. Once the sample workflow had been thoroughly validated, it was applied to the concomitant quantification of total ApoE and ApoE4 isoform in a large case-control study (n=669). The final measurements were consistent with most previously reported ApoE concentration values and confirm the influence of the different alleles on the protein expression level. Our results illustrate (i) the reliability of selected reaction monitoring-based assays and (ii) the value of the oxidization step for unbiased monitoring of methionine-containing proteotypic peptides. Furthermore, a statistical analysis indicated that neither total ApoE and ApoE4 levels nor the ApoE/ApoE4 ratio correlated with the diagnosis of AD. These findings reinforce the conclusions of previous studies in which plasma ApoE levels had no obvious clinical significance.

Answer:
Which ApoE isoform is associated with atherosclerosis and Alzheimer's disease?