In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".
--------
Question: Paragraph: Objective: Tumor-treating fields are currently used to successfully treat various cancers; however, the specific pathways associated with its efficacy remain unknown in the immune responses. Here, we evaluated tumor-treating fields-mediated initiation of the macrophage-specific immune response. Materials and Methods: We subjected RAW 264.7 mouse macrophages to clinically relevant levels of tumor-treating fields (0.9 V/cm, 150 kHz) and evaluated alterations in cytokine expression and release, as well as cell viability. Additionally, we investigated the status of immunomodulatory pathways to determine their roles in tumor-treating fields-mediated immune activation. Results and Discussion: Our results indicated that tumor-treating fields treatment at 0.9 V/cm decreased cell viability and increased cytokine messenger RNA/protein levels, as well as levels of nitric oxide and reactive oxygen species, relative to controls. The levels of tumor necrosis factor a, interleukin 1b, and interleukin 6 were markedly increased in tumor-treating fields-treated RAW 264.7 cells cocultured with 4T1 murine mammary carcinoma cells compared with those in 4T1 or RAW 264.7 cells with or without tumor-treating fields treatment. Moreover, the viability of 4T1 cells treated with the conditioned medium of tumor-treating fields-stimulated RAW 264.7 cells decreased, indicating that macrophage activation by tumor-treating fields effectively killed the tumor cells. Moreover, tumor-treating fields treatment activated the nuclear factor kB and mitogen-activated protein kinase pathways involved in immunomodulatory signaling. Conclusion: These results provide critical insights into the mechanisms through which tumor-treating fields affect macrophagespecific immune responses and the efficacy of this method for cancer treatment. 
 Title: Tumor-Treating Fields Induce RAW264.7 Macrophage Activation Via NK-kB/MAPK Signaling Pathways

Answer: True


Question: Paragraph: The rapid improvements in DNA synthesis technology hold the potential to revolutionize biosciences in the near future. Traditional genetic engineering methods are template dependent and make extensive but laborious use of site-directed mutagenesis to explore the impact of small variations on an existing sequence ''theme.'' De novo gene and genome synthesis frees the investigator from the restrictions of the pre-existing template and allows for the rational design of any conceivable new sequence theme. Viruses, being among the simplest replicating entities, have been at the forefront of the advancing biosciences since the dawn of molecular biology. Viral genomes, especially those of RNA viruses, are relatively short, often less than 10,000 bases long, making them amenable to whole genome synthesis with the currently available technology. For this reason viruses are once again poised to lead the way in the budding field of synthetic biology-for better or worse. 
 Title: Putting Synthesis into Biology: A Viral View of Genetic Engineering through De Novo Gene and Genome Synthesis

Answer: True


Question: Paragraph: Diagnostic electron microscopy (DEM) was an essential component of viral diagnosis until the development of highly sensitive nucleic acid amplification techniques (NAT). The simple negative staining technique of DEM was applied widely to smallpox diagnosis until the world-wide eradication of the human-specific pathogen in 1980. Since then, the threat of smallpox re-emerging through laboratory escape, molecular manipulation, synthetic biology or bioterrorism has not totally disappeared and would be a major problem in an unvaccinated population. Other animal poxviruses may also emerge as human pathogens. With its rapid results (only a few minutes after arrival of the specimen), no requirement for specific reagents and its "open view," DEM remains an important component of virus diagnosis, particularly because it can easily and reliably distinguish smallpox virus or any other member of the orthopoxvirus (OPV) genus from parapoxviruses (PPV) and the far more common and less serious herpesviruses (herpes simplex and varicella zoster). Preparation, enrichment, examination, internal standards and suitable organisations are discussed to make clear its continuing value as a diagnostic technique. in childhood, it was clear that chickenpox was what he now had. No national alert was necessary, with all the widespread anxiety that would have followed. The speed and certainty of the diagnosis by EM offered a lesson that still has relevance. Four valuable lessons can be drawn from this episode: (1) That a useful diagnosis can be made in minutes from the arrival of the specimen; (2) Diagnostic EM (DEM) required no specific reagents (such as primers or antisera) or special equipment. All that is required was an electron microscope, microscope support grids, stain and a competent virologist familiar with the appearance of relevant viruses; (3) That seeing is believing-knowing what the causative virus looks like is a useful confirmation for any other tests added later; (4) That other, more serious, causes could be discounted. With concerns over the possible re-emergence of poxvirus infections, either through the spread of existing animal viruses to susceptible humans [1] [2] [3] [4] [5] , survival and escape of old material [6-8], molecular manipulation and synthetic biology [9] or even bioterrorism [10-12], there will be a need to provide accurate and quick diagnosis. This paper presents the possibilities and advantages of DEM and how DEM can help with organised future preparedness. Viruses 2018, 10, x 8 of 30 at Library of the RKI for help with the acquisition of published information, and Lars Möller, ZBS 4, RKI for his kind help to prepare an online-version of our manuscript. Thanks are also due to Anna-M. Eis-Hübinger, Bonn, and Bernhard Pfeiff for information on the fatal CPXV case and kindly providing images. We acknowledge the generous help by Gudrun Wibbelt, IZW Berlin, Pierre Formenty, WHO Geneva, and Cynthia Goldsmith, CDC, with the acquisition of clinical images of CPXV and the MPXV zoonoses respectively. Hermann Meyer, Munich, supplied hg readily with inactivated MPXV samples from the DRC. For supplying diagnostic PPV samples repeatedly, we thank Klaus Eisendle, Bolzano and Friedrich A. Bahmer, Bremen. Thanks are finally due to the many clinical colleagues we had the pleasure to work with over some 60 years all together. 
 Title: viruses Rapid Viral Diagnosis of Orthopoxviruses by Electron Microscopy: Optional or a Must?

Answer:
False