Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

Input: Consider Input: In plant, primary transcripts (pri-miRNAs) transcribed from miRNA genes by RNA polymerase II are first processed into stem-loop pre-miRNAs and further chopped into 21nt long miRNAs by RNase III-like enzyme DCL1. SERRATE (SE) protein is an essential component for miRNA processing by assisting DCL1 for accurate cleavage. Here we report the crystal structure of Arabidopsis SE core (residues 194-543) at 2.7A. SE core adopts the 'walking man-like' topology with N-terminal helices, C-terminal non-canonical zinc-finger domain and novel Middle domain resembling the leading leg, the lagging leg and the body, respectively. Pull-down assay shows that SE core provides the platform for HYL1 and DCL1 binding, whereas in vitro RNA binding and in vivo mutant rescue experiments suggest that the non-canonical zinc-finger domain coupled with C-terminal tail binds miRNA precursors. SE presumably works as a scaffold-like protein capable of binding both protein and RNA to guide the positioning of miRNA precursor toward DCL1 catalytic site within miRNA processing machinery in plant.

Output: Which polymerase transcribes pri-miRNAs?


Input: Consider Input: 1. In order to investigate the biological function of the human CLN3 gene that is defective in Batten disease, we created a yeast strain by PCR-targeted disruption of the yeast gene (YHC3), which is a homologue of the human CLN3 gene. 2. The phenotypic characterization revealed that the yhc3 delta mutants are more sensitive to combined heat and alkaline stress than the wild-type strains as determined by inhibition of cell proliferation. 3. This suggests that the yhc3 delta mutant is a good model to investigate the biological function of human CLN3 gene in mammalian cells and to understand the pathophysiology of juvenile Batten disease.

Output: What is the effect of a defective CLN3 gene?


Input: Consider Input: Organisms that live in lakes, ponds, streams, springs or wetlands are part of freshwater ecosystems. These ecosys- tems vary by temperature, pressure (in lakes), the amount of light that penetrates and the type of vegetation that lives there. Limnology is the study of bodies of fresh water and the organisms that live there. A lake has zones just like the ocean. The ecosystem of a lake is divided into three distinct zones (Figure 1.1): 1. The surface (littoral) zone is the sloped area closest to the edge of the water. 2. The open-water zone (also called the photic or limnetic zone) has abundant sunlight. 3. The deep-water zone (also called the aphotic or profundal zone) has little or no sunlight. There are several life zones found within a lake: In the littoral zone, sunlight promotes plant growth, which provides food and shelter to animals such as snails, insects, and fish. In the open-water zone, other plants and fish, such as bass and trout, live. The deep-water zone does not have photosynthesis since there is no sunlight. Most deep-water organisms are scavengers, such as crabs and catfish that feed on dead organisms that fall to the bottom of the lake. Fungi and bacteria aid in the decomposition in the deep zone. Though different creatures live in the oceans, ocean waters also have these same divisions based on sunlight with similar types of creatures that live in each of the zones. The three primary zones of a lake are the littoral, open-water, and deep-water zones. Wetlands are lands that are wet for significant periods of time. They are common where water and land meet. Wetlands can be large flat areas or relatively small and steep areas. Wetlands are rich and unique ecosystems with many species that rely on both the land and the water for survival. Only specialized plants are able to grow in these conditions. Wetlands tend have a great deal of biological diversity. Wetland ecosystems can also be fragile systems that are sensitive to the amount and quality of water present within them. Click image to the left or use the URL below. URL: Marshes are shallow wetlands around lakes, streams, or the ocean where grasses and reeds are common, but trees are not (Figure 1.2). Frogs, turtles, muskrats, and many varieties of birds are at home in marshes. A salt marsh on Cape Cod in Mas- sachusetts. A swamp is a wetland with lush trees and vines found in low-lying areas beside slow-moving rivers (Figure 1.3). Like marshes, they are frequently or always inundated with water. Since the water in a swamp moves slowly, oxygen in the water is often scarce. Swamp plants and animals must be adapted for these low-oxygen conditions. Like marshes, swamps can be fresh water, salt water, or a mixture of both. As mentioned above, wetlands are home to many different species of organisms. Although they make up only 5% of the area of the United States, wetlands contain more than 30% of the plant types. Many endangered species live in wetlands, so wetlands are protected from human use. Wetlands also play a key biological role by removing pollutants from water. For example, they can trap and use fertilizer that has washed off a farmers field, and therefore they prevent that fertilizer from contaminating another body of water. Since wetlands naturally purify water, preserving wetlands also helps to maintain clean supplies of water.


Output: marshes are distinctive because