In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".

Paragraph: Retroviruses are classified as exogenous and endogenous retroviruses according to the mode of transmission. Endogenous retroviruses (ERVs) are retroviruses which have been integrated into germ-line cells and inherited from parents to offspring. Most ERVs are inactivated by deletions and mutations; however, certain ERVs maintain their infectivity and infect the same host and new hosts as exogenous retroviruses. All domestic cats have infectious ERVs, termed RD-114 virus. Several canine and feline attenuated vaccines are manufactured using RD-114 virus-producing cell lines such as Crandell-Rees feline kidney cells; therefore, it is possible that infectious RD-114 virus contaminates live attenuated vaccines. Recently, Japanese and UK research groups found that several feline and canine vaccines were indeed contaminated with infectious RD-114 virus. This was the first incidence of contamination of 'infectious' ERVs in live attenuated vaccines. RD-114 virus replicates efficiently in canine cell lines and primary cells. Therefore, it is possible that RD-114 virus infects dogs following inoculation with contaminated vaccines and induces proliferative diseases and immune suppression, if it adapts to grow efficiently in dogs. In this review, we summarize the incidence of contamination of RD-114 virus in live attenuated vaccines and potential risks of infection with RD-114 virus in dogs. Retroviruses enter host cells via binding of the envelope proteins with the host receptor(s). After entering the cells, viral RNA is reverse-transcribed into DNA and then the DNA is integrated into the host genome to be a provirus. Viral RNA is transcribed from the provirus and the structural and enzymatic proteins of the virus are synthesized from the transcribed viral RNA. Retroviruses are classified as exogenous and endogenous retroviruses according to the mode of transmission [14] . Usually, exogenous retroviruses infect somatic cells but not germ-line cells, and they are transmitted horizontally by infection via viral particles. On the other hand, ERVs are retroviruses which have been integrated into germ-line cells. ERVs behave like normal genes and are inherited from parents to offspring as Mendelian's law. ERVs occupy about 10 % of mammalian genomes and are mostly inactivated by deletions and mutations [14, 15, 17, 27] . However, a limited number of ERVs maintain their infectivity and infect new hosts as exogenous retroviruses [6] . Exogenous retroviruses are classified into seven genera, i.e., alpharetrovirus, betaretrovirus, gammaretrovirus, deltaretrovirus, epsilonretrovirus, spumaretrovirus and Electronic supplementary material The online version of this article ( 
 Title: Contamination of live attenuated vaccines with an infectious feline endogenous retrovirus (RD-114 virus)
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Paragraph: cDNA, synthesized on bovine coronavirus (BCV) genomic RNA templates, could be used to detect very small quantities (i .e . 1 pg) of viral RNA by hybridization with either radioisotopic-labelled or biotinylated recombinant plasmids . Virus was optimally attached to nitrocellulose membranes when spotted in 1 x SSC, whereas 20 x SSC was superior for viral RNA. Denaturation and RNA fixation of both RNA, still encapsidated in virus particles and isolated genomic RNA, was achieved by baking of the blots in vacuum . Virus detection in the supernatant of infected HRT-18 cells was feasible, but improved significantly after proteinase K treatment . No homology was observed between virus cDNA with either plasmid DNA or nucleic acid isolated from non-infected HRT-18 cells . Hybridization with radioisotopic-labelled probes in higher formamide concentrations (up to 60%) increased the detection signals, possibly by reducing reassociation of the probe . Significant detection amplification (30-50 times) was achieved in the case of biotinylated probes by stimulation of hyperpolymer formation on already hybridized target sequences, by additional hybridization with biotinylated pUC-19 . A detection amplification was also obtained when hybridization was done with two probes (pBC-52 and pBC-247), containing non-overlapping viral sequences . Although the detectability was surpassed by biotinylated probes, sensitivity was superior in radioisotopic virus detection . 
 Title: Biotinylated and radioactive cDNA probes in the detection by hybridization of bovine enteric coronavirus
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Paragraph: Background: Severe pneumonia is responsible for great mortality and morbidity worldwide, and early-applied effective antiinfective therapy can improve the prognosis of patients. However, identification of infectious agents in severe pneumonia remains a major challenge so far. In this study, the potential utility of transmission electron microscopy (TEM) in detecting nonbacterial pathogens in patients with severe pneumonia was retrospectively evaluated. Materials and Methods: A total of 106 patients diagnosed with severe pneumonia at our hospital from September 2015 to December 2017 were included, and their baseline clinical characteristics were collected. Nonbacterial infectious agents detected by TEM in bronchoalveolar lavage fluid (BALF) and serological tests were summarized. The detection rates were further compared between TEM and serological tests. Results: BALF examination under the transmission electron microscope revealed 24 viruses, 16 mycoplasmas, 18 chlamydia, 2 fungi and 74 bacteria in 99 samples, among which 61 samples were mixed infections. The combined use of serological tests and TEM significantly improved the detection rate of nonbacterial infectious agents in patients with severe pneumonia. Conclusions: Our data support that implementation of TEM could improve the sensitivity for detecting viruses, atypical pathogens and mixed infections in BALF from patient of severe pneumonia. Therefore, TEM may be used as an auxiliary diagnostic method of other microbiological tests in severe pneumonia. 
 Title: Transmission Electron Microscopy Improves the Diagnostic Sensitivity in Nonbacterial Etiology of Severe Pneumonia: A Retrospective Study

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