In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Q: The presence of immunoglobulin oligoclonal bands in the cerebrospinal fluid of Multiple Sclerosis (MS) patients supports the hypothesis of an infectious etiology, although the antigenic targets remain elusive. Neurotropic mouse hepatitis virus (MHV) infection in mice provides a useful tool for studying mechanisms of demyelination in a virus-induced experimental model of MS. This study uses Affymetrix microarray analysis to compare differential spinal cord mRNA levels between mice infected with demyelinating and non-demyelinating strains of MHV to identify host immune genes expressed in this demyelinating disease model. The study reveals that during the acute stage of infection, both strains induce inflammatory innate immune response genes, whereas upregulation of several immunoglobulin genes during chronic stage infection is unique to infection with the demyelinating strain. Results suggest that the demyelinating strain induced an innate-immune response during acute infection that may promote switching of Ig isotype genes during chronic infection, potentially playing a role in antibody-mediated progressive demyelination even after viral clearance.

A: Demyelinating strain of mouse hepatitis virus infection bridging innate and adaptive immune response in the induction of demyelination
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Q: Although single cell RNA sequencing studies have begun providing compendia of cell expression profiles, it has proven more difficult to systematically identify and localize all molecular cell types in individual organs to create a full molecular cell atlas. Here we describe droplet-and plate-based single cell RNA sequencing applied to ~75,000 human lung and blood cells, combined with a multi-pronged cell annotation approach, which have allowed us to define the gene expression profiles and anatomical locations of 58 cell populations in the human lung, including 41 of 45 previously known cell types or subtypes and 14 new ones. This comprehensive molecular atlas elucidates the biochemical functions of lung cell types and the cell-selective transcription factors and optimal markers for making and monitoring them; defines the cell targets of circulating hormones and predicts local signaling interactions including sources and targets of chemokines in immune cell trafficking and expression changes on lung homing; and identifies the cell types directly affected by lung disease genes and respiratory viruses. Comparison to mouse identified 17 molecular types that appear to have been gained or lost during lung evolution and others whose expression profiles have been substantially altered, revealing extensive plasticity of cell types and cell-type-specific gene expression during organ evolution including expression switches between cell types. This atlas provides the molecular foundation for investigating how lung cell identities, functions, and interactions are achieved in development and tissue engineering and altered in disease and evolution. All rights reserved. No reuse allowed without permission.

A: A molecular cell atlas of the human lung from single cell RNA sequencing
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Q: ABSTRCT 29 Given the scale and rapid spread of severe acute respiratory syndrome coronavirus 2 30 (SARS-CoV-2, known as 2019-nCov) infection , the ongoing global 31 SARS-CoV-2 outbreak has become a huge public health issue. Rapid and precise 32 diagnostic methods are thus immediately needed for diagnosing COVID-19, 33 providing timely treatment and facilitating infection control. A one-step reverse 34 transcription loop-mediated isothermal amplification (RT-LAMP) coupled with 35 nanoparticles-based biosensor (NBS) assay (RT-LAMP-NBS) was successfully 36 established for rapidly and accurately diagnosing COVID-19. A simple equipment 37 (such as heating block) was required for maintaining a constant temperature (63°C) 38 for only 40 min. Using two designed LAMP primer sets, F1ab (opening reading frame 39 1a/b) and np (nucleoprotein) genes of SARS-CoV-2 were simultaneously amplified 40 and detected in a 'one-step' and 'single-tube' reaction, and the detection results were 41 easily interpreted by NBS. The sensitivity of SARS-CoV-2 RT-LAMP-NBS was 12 42 copies (each of detection target) per reaction, and no cross-reactivity was generated 43 from non-SARS-CoV-2 templates. Among clinically diagnosed COVID-19 patients, 44 the analytical sensitivity of SARS-CoV-2 was 100% (33/33) in the oropharynx swab 45 samples, and the assay's specificity was also 100% (96/96) when analyzed the clinical 46 samples collected from non-COVID-19 patients. The total diagnosis test from sample 47 collection to result interpretation only takes approximately 1 h. In sum, the 48 RT-LAMP-NBS is a promising tool for diagnosing the current SARS-CoV-2 49 infection in first line field, public health and clinical laboratories, especially for 50 resource-challenged regions. 51

A:
Title Page: 1 Reverse transcription loop-mediated isothermal amplification combined with 2 nanoparticles-based biosensor for diagnosis of COVID-19 3 4 Laboratory of Pediatric Respiratory Infection Disease, National Clinical Research
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