instruction:
In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".
question:
Paragraph: While autophagy has been shown to act as an anti-viral defense, the Picornaviridae avoid and, in many cases, subvert this pathway to promote their own replication. Evidence indicates that some picornaviruses hijack autophagy in order to induce autophagosome-like membrane structures for genomic RNA replication. Expression of picornavirus proteins can specifically induce the machinery of autophagy, although the mechanisms by which the viruses employ autophagy appear to differ. Many picornaviruses up-regulate autophagy in order to promote viral replication while some members of the family also inhibit degradation by autolysosomes. Here we explore the unusual relationship of this medically important family of viruses with a degradative mechanism of innate immunity. 
 Title: Picornavirus Subversion of the Autophagy Pathway
answer:
True


question:
Paragraph: The NIAID (National Institute for Allergy and Infectious Diseases) Biodefense Proteomics program aims to identify targets for potential vaccines, therapeutics, and diagnostics for agents of concern in bioterrorism, including bacterial, parasitic, and viral pathogens. The program includes seven Proteomics Research Centers, generating diverse types of pathogen-host data, including mass spectrometry, microarray transcriptional profiles, protein interactions, protein structures and biological reagents. The Biodefense Resource Center (www.proteomicsresource.org) has developed a bioinformatics framework, employing a protein-centric approach to integrate and support mining and analysis of the large and heterogeneous data. Underlying this approach is a data warehouse with comprehensive protein + gene identifier and name mappings and annotations extracted from over 100 molecular databases. Value-added annotations are provided for key proteins from experimental findings using controlled vocabulary. The availability of pathogen and host omics data in an integrated framework allows global analysis of the data and comparisons across different experiments and organisms, as illustrated in several case studies presented here. (1) The identification of a hypothetical protein with differential gene and protein expressions in two host systems (mouse macrophage and human HeLa cells) infected by different bacterial (Bacillus anthracis and Salmonella typhimurium) and viral (orthopox) pathogens suggesting that this protein can be prioritized for additional analysis and functional characterization. (2) The analysis of a vaccinia-human protein interaction network supplemented with protein accumulation levels led to the identification of human Keratin, type II cytoskeletal 4 protein as a potential therapeutic target. (3) Comparison of complete genomes from pathogenic variants coupled with experimental information on complete proteomes allowed the identification and prioritization of ten potential diagnostic targets from Bacillus anthracis. The integrative analysis across data sets from multiple centers can reveal potential functional significance and hidden relationships between pathogen and host proteins, thereby providing a systems approach to basic understanding of pathogenicity and target identification. 
 Title: Systems Integration of Biodefense Omics Data for Analysis of Pathogen-Host Interactions and Identification of Potential Targets
answer:
False


question:
Paragraph: MBP-tagged TRIM25 protein was expressed in E.coli Rosetta 2 (DE3) TM cells (Millipore). Transformed cells were grown in LB broth containing 0.2% glucose at 37 °C. Then, 0.2 mM IPTG was added and the cells were incubated under shaking conditions for 18 h at 25 °C, after which the cells were harvested by centrifugation. The pellet was resuspended in T1 buffer (20 mM Tris/Cl(pH 7.4), 1 mM EDTA, 1 mM sodium azide, and 1 mM DTT) and sonicated. After centrifugation, the supernatant was loaded onto a DEAE ion-exchange column (GE healthcare) and the flow throw was collected. Next, the pooled sample was applied to amylose resin (NEB). The column was washed with T2 buffer (T1 buffer + 200 mM NaCl) and eluted with T3 buffer (T2 buffer + 10 mM maltose). The purity of the proteins was analyzed by 10% SDS-PAGE. The final yield of MBP-TRIM25 was determined by the Bradford assay with BSA as the standard and the samples were stored at �80 °C. His-tagged RIG-I was expressed in E. coli BL21(DE3) cells. The transformed cells were grown in LB broth at 37 °C and then further incubated at 4 °C for 3 h. Subsequently, 0.25 mM IPTG was added and the cells were incubated under shaking conditions for 18 h at 25 °C, after which the cells were harvested by centrifugation. The pellet was resuspended in R1 buffer (50 mM HEPES; pH 7.4, 10% glycerol, 500 mM NaCl, 0.2% NP-40, 10 mM imidazole, 1 mM PMSF, 0.5 mg/ml lysozyme, and protease inhibitor cocktail) and sonicated. After centrifugation, the supernatant was applied to a Ni-NTA chelating agarose (Incospham). The column was washed with R2 buffer (50 mM HEPES; pH 7.4, 10% glycerol, 500 mM NaCl, 0.2% NP-40, and 50 mM imidazole) and the His-tagged RIG-I was eluted by increasing the imidazole concentration. The purest fractions were applied to a heparin-Sepharose column (GE healthcare), and the resin was washed with R3 buffer (50 mM Tris/Cl(pH 7.4), 10% glycerol, 0.2% NP-40, and 20 mM NaCl) and eluted with increasing concentrations of NaCl (up to 500 mM). The final yield of His-RIG-I was determined by the Bradford assay (Bio-Rad) with BSA as the standard. RNA Aptamers Specific to NS1 Protein of Influenza Virus Hye-Min Woo et al. 
 Title: Molecules and Cells Supplementary Materials
answer:

False