In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

[Q]: All coronaviruses (CoVs), including the causative agent of severe acute respiratory syndrome (SARS), encode a nucleocapsid (N) protein that harbors two independent RNA binding domains of known structure, but poorly characterized RNA binding properties. We show here that the Nterminal domain (NTD) of N protein from mouse hepatitis virus (MHV), a virus most closely related to SARS-CoV, employs aromatic amino acidnucleobase stacking interactions with a triple adenosine motif to mediate high-affinity binding to single-stranded RNAs containing the transcriptional regulatory sequence (TRS) or its complement (cTRS). Stoichiometric NTD fully unwinds a TRS-cTRS duplex that mimics a transiently formed transcription intermediate in viral subgenomic RNA synthesis. Mutation of the solvent-exposed Y127, positioned on the β-platform surface of our 1.75 Å structure, binds the TRS far less tightly and is severely crippled in its RNA unwinding activity. In contrast, the C-terminal domain (CTD) exhibits no RNA unwinding activity. Viruses harboring Y127A N mutation are strongly selected against and Y127A N does not support an accessory function in MHV replication. We propose that the helix melting activity of the coronavirus N protein NTD plays a critical accessory role in subgenomic RNA synthesis and other processes requiring RNA remodeling.
[A]: Coronavirus N Protein N-Terminal Domain (NTD) Specifically Binds the Transcriptional Regulatory Sequence (TRS) and Melts TRS-cTRS RNA Duplexes


[Q]: Background: Respiratory syncytial virus (RSV), human Rhinovirus (HRV) and human Metapneumo Virus (HMPV) are important viral pathogens causing acute respiratory tract infections in the hospitalized patients. Sensitive and accurate detection of RSV, HRV and HMPV is necessary for clinical diagnosis and treatment. Results: A locked nucleic acid (LNA)-based multiplex closed one-tube nested real-time RT-PCR (mOTNRT-PCR) assay was developed for simultaneous detection of RSV, HRV and HMPV. The sensitivity, specificity, reproducibility and clinical performance of mOTNRT-PCR were evaluated and compared with individual real time PCR (RT-qPCR) assay using clinical samples. The analytical sensitivity of mOTNRT-PCR assay was 5 copies/reaction for RSV, HRV and HMPV, respectively, and no cross-reaction with other common respiratory viruses was observed. The coefficients of variation (CV) of intra-assay and inter-assay were between 0.51 to 3.67%. Of 398 nasopharyngeal aspirates samples tested, 109 (27.39%), 150 (37.69%) and 44 (11.06%) were positive for RSV, HRV and HMPV, respectively, whereas 95 (23.87%), 137 (34.42%) and 38 (9.55%) were positive for RSV, HRV and HMPV, respectively, by individual RT-qPCR assay. Thirty three samples that were positive by mOTNRT-PCR but negative by RT-qPCR were confirmed as true positives by sequencing using reported traditional two-step nested PCR assay. Conclusion: mOTNRT-PCR assay reveals extremely higher sensitivity than that of RT-qPCR assay for detecting RSV, HRV and HMPV in clinical settings.
[A]: A multiplex one-tube nested real time RT- PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus


[Q]: 1 transmission routes 2 Sk Shahid Nadim a , Indrajit Ghosh 1a , Joydev Chattopadhyay a 3 Abstract 5 In this paper, we study the dynamics of a vector-borne disease model with two transmission paths: direct transmission through contact and indirect transmission through vector. The direct transmission is considered to be a non-monotone incidence function to describe the psychological effect of some severe diseases among the population when the number of infected hosts is large and/or the disease possesses high case fatality rate. The system has a disease-free equilibrium which is locally asymptomatically stable when the basic reproduction number (R 0 ) is less than unity and may have up to four endemic equilibria. Analytical expression representing the epidemic growth rate is obtained for the system. Sensitivity of the two transmission pathways were compared with respect to the epidemic growth rate. We numerically find that the direct transmission coefficient is more sensitive than the indirect transmission coefficient with respect to R 0 and the epidemic growth rate. Local stability of endemic equilibria is studied. Further, the global asymptotic stability of the endemic equilibrium is proved using Li and Muldowney geometric approach. The explicit condition for which the system undergoes backward bifurcation is obtained. The basic model also exhibits the hysteresis phenomenon which implies diseases will persist even when R 0 < 1 although the system undergoes a forward bifurcation and this phenomenon is rarely observed in disease models. Consequently, our analysis suggests that the diseases with multiple transmission routes exhibit bi-stable dynamics. However, efficient application of temporary control in bi-stable regions will curb the disease to lower endemicity. In addition, increase in transmission heterogeneity will increase the chance of disease eradication. 7 12 directly by person-to-person contact and indirectly through a vector. Understanding the transmission 13 dynamics of such diseases is of utmost interest. Mathematical models of epidemic diseases have been 14 very insightful in uncovering epidemiology and transmission patterns of the diseases. It is very important 15 to understand such epidemic patterns for better public health interventions and control of outbreaks. 16 Another important objective of these models is to inform public health organizations about the effect of 17 control policies on a certain population. However, hosts behaviour and psychological factors are some-18 times the main drivers of further transmission of the disease. For example, the epidemic outbreak of 19 severe acute respiratory syndrome (SARS) (3; 4) had such psychological effects and individual protection 20 1 doi: bioRxiv preprint 32 However, the dynamics of an epidemic model with two transmission routes where the direct trans-33 mission incorporates psychological effect has not been studied so far. The ongoing MERS-CoV epidemic 34 and zika virus epidemic are examples of diseases with two routes of transmission having psychological 35 effects. The psychosocial implication on the disease outbreak is more dangerous than any other phys-36 ical effect. West Nile Virus outbreak in the US is a good example for the onset of psychogenic illness 37 (10) and may generate mistrust towards national or health authorities. For example, the mild effects 38 of Zika virus, there is a correlation between psychosis with fetus microcephaly where pregnant women 39 and their relatives are highly sensitive group. Therefore, there is a negative correlation between the 40 psychological effect and disease prevalence and hence we consider non-monotone incidence function i.e 41 p < q to describe the direct transmission between hosts in our study. This type of function can be used 42 to interpret the "psychological effects" (11). We use the incidence rate g(I)S = βIS 1+αI 2 where βI repre-43 sent the force of infection of the diseases, 1 1+αI 2 represent the psychological effect from the behavioral 44 changes of susceptible population when the number of infected populations increases. Our goal is to 45 develop a simple mathematical model that capture a range of disease dynamics with multiple mode of 46 transmissions. Moreover, we would like to perform a detailed stability and bifurcation analysis of the 47 proposed model. By using analytical methods we intend to uncover the dynamical properties of the 48 diseases with two routes of transmission. The rest of the paper is organized as follows: Section 2 consist of the model formulation and the 50 basic assumptions. In Section 3, preliminary mathematical analysis of the proposed model is carried out. Section 4 is devoted to the global stability of the endemic equilibrium using geometric approach while 52 in Section 5 backward bifurcation and forward bifurcation with hysteresis effect is presented. In Section 53 6 we investigate some numerical scenarios such as effect of temporary control on bistable dynamics, 54 effect of the strength of psychological fear and impact of heterogeneity in indirect transmission. Finally, 55 discussion and conclusion is presented in Section 7. 56 2. Model formulation 57 We consider two population groups as host and vector population. Time-dependent state variables 58 are taken to describe the compartments of the two populations. Let at time t, N h (t) represents total 59 host population and N v (t) represents total vector population. We consider a 'SIR' type model for the 60 host population and a 'SI' type model for the vectors. The host population is subdivided into three 61 mutually disjoint classes: susceptible, S h ; infected, I h ; recovered, R h . Thus, at any time t, the size of 62 the host population is given by N h = S h + I h + R h . The vector population consist of two classes namely, 63 susceptible vectors, S v ; and infected vectors, I v . With this division, the size of the vector population 64 2 . CC-BY 4.0 The basic reproduction number, R 0 , is the number of secondary infections which one infected indi-102 vidual would produce in a entirely susceptible population. The disease free equilibrium (DFE) of the 103 system is given by Y 0 = ( Π h µ h , 0, 0, Πv µv , 0). There are two infected compartments in the model (2.1), I h 104 and I v . R 0 can be derived from the jacobian matrix of the system (2.1) calculated at Y 0 together with 105 the assumption of local asymptotic stability of Y 0 and is given by
[A]:
Global dynamics of a general vector-borne disease model with two