In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

The emergence of the severe acute respiratory syndrome coronavirus (SARS-CoV) has led to a renewed interest in studying the role of accessory proteins in regulating coronavirus infections in the natural host. A significant body of evidence has accumulated in the area of SARS-CoV and host interactions that indicate that the accessory proteins might play an important role in modulating the host response to virus infection and thereby, contribute to pathogenesis. In this review, we have compiled the current knowledge about SARS-CoV accessory proteins, obtained from studies in cell culture systems, reverse genetics and animal models, to shed some light into the possible role of these proteins in the propagation and virulence of SARS-CoV in its natural host. We conclude by providing some questions for future studies that will greatly advance our knowledge about the biological significance and contributions of the accessory proteins in the development of SARS in humans.
SARS coronavirus accessory proteins

Coronavirus reverse genetic systems have become valuable tools for studying the molecular biology of coronavirus infections. They have been applied to the generation of recombinant coronaviruses, selectable replicon RNAs, and coronavirus-based vectors for heterologous gene expression. Here we provide a collection of protocols for the generation, cloning, and modification of full-length coronavirus cDNA using vaccinia virus as a cloning vector. Based on cloned coronaviral cDNA, we describe the generation of recombinant coronaviruses and stable cell lines containing coronaviral replicon RNAs. Initially, the vaccinia virus-based reverse genetic system was established for the generation of recombinant human coronavirus 229E. However, it is also applicable to the generation of other coronaviruses, such as the avian infectious bronchitis virus, mouse hepatitis virus, and SARS coronavirus.
Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs

Temperature-sensitive mutants of the munne coronavirus JHM induced a sub~ acute demyelinatin 8 encephalomyelitis (SD~E) in youn~ rats. Neurological symptol~,; were associated with marked lesion3 of primary d~my¢;lination ".m the white matter of the central nervous system (CNS), and developing after an incubation time of several weeks to months. Many rats survived this infection and rco3vered completely from this CNS disease. Amon 8 43 survivors of SDE, 9 ra'~s developed a ~'elapse 27-153 days after onset of the first attack. Neuropathologica.l ex~n~ation ol~ these animals revealed areas of fresh| ¢~emyelination together with old remyelinated lesions. V'md antisens were detectable in the neighbourhood of fresh les:ions and in some ca~ infectious virus was re-i~lated from rats revealing low antibl~y titers to JHM virus. These results demonstrate that mutants of JHM virus can induca~ a relapsing demyelinating disease process, associated with a persistent in fection, which some similarities to chronic experimental allergic enceph~dor~ayel/tis.

Relapsing Subacute Demyelina~:ing Encephalomyelitis in Rats during the Course of Coronavirus JHM Infection