Given a question and a context passage, generate the answer having the word or phrase from the context passage. Here, the answer should be a shortest continous span from the passage.

Q: Context: In eukaryotic cells, there are two sub-pathways of nucleotide excision repair (NER), the global genome (gg) NER and the transcription-coupled repair (TCR). TCR can preferentially remove the bulky DNA lesions located at the transcribed strand of a transcriptional active gene more rapidly than those at the untranscribed strand or overall genomic DNA. This strand-specific repair in a suitable restriction fragment is usually determined by alkaline gel electrophoresis followed by Southern blotting transfer and hybridization with an indirect end-labeled single-stranded probe. Here we describe a new method of TCR assay based on strand-specific-PCR (SS-PCR). Using this method, we have investigated the role of DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase-related protein kinases (PIKK) family, in the TCR pathway of UV-induced DNA damage. Although depletion of DNA-PKcs sensitized HeLa cells to UV radiation, it did not affect the ggNER efficiency of UV-induced cyclobutane pyrimidine dimers (CPD) damage. We postulated that DNA-PKcs may involve in the TCR process. To test this hypothesis, we have firstly developed a novel method of TCR assay based on the strand-specific PCR technology with a set of smart primers, which allows the strand-specific amplification of a restricted gene fragment of UV radiation-damaged genomic DNA in mammalian cells. Using this new method, we confirmed that siRNA-mediated downregulation of Cockayne syndrome B resulted in a deficiency of TCR of the UV-damaged dihydrofolate reductase (DHFR) gene. In addition, DMSO-induced silencing of the c-myc gene led to a decreased TCR efficiency of UV radiation-damaged c-myc gene in HL60 cells. On the basis of the above methodology verification, we found that the depletion of DNA-PKcs mediated by siRNA significantly decreased the TCR capacity of repairing the UV-induced CPDs damage in DHFR gene in HeLa cells, indicating that DNA-PKcs may also be involved in the TCR pathway of DNA damage repair. By means of immunoprecipitation and MALDI-TOF-Mass spectrometric analysis, we have revealed the interaction of DNA-PKcs and cyclin T2, which is a subunit of the human transcription elongation factor (P-TEFb). While the P-TEFb complex can phosphorylate the serine 2 of the carboxyl-terminal domain (CTD) of RNA polymerase II and promote transcription elongation. A new method of TCR assay was developed based the strand-specific-PCR (SS-PCR). Our data suggest that DNA-PKcs plays a role in the TCR pathway of UV-damaged DNA. One possible mechanistic hypothesis is that DNA-PKcs may function through associating with CyclinT2/CDK9 (P-TEFb) to modulate the activity of RNA Pol II, which has already been identified as a key molecule recognizing and initializing TCR. 
 Question: Which gene strand is targeted by transcription-coupled repair (TCR)?

A: the transcribed strand
****
Q: Context: New England, the late 1950s. Todd Anderson (Ethan Hawke), a lonely and painfully shy teenager, who is under pressure by his stern parents because he must live up to his older brother's reputation to attend Yale and become a lawyer, arrives for the new semester at the Welton Academy for boys -- Todd's brother also attended Welton and was a popular and well-regarded student there. This semester begins during an orientation gathering with a speech given by the stern Headmaster Nolan (Norman Lloyd), who states the academy's four pillars: Tradition, Honor, Discipline, and Excellence. Todd meets Neil Perry (Robert Sean Leonard) an ambitious student whom becomes his dorm roommate.Later in his dorm, Neil is ordered by his grumpy and domineering father (Kurtwood Smith) to drop his involvement with the school annual in order to maintain good grades so the boy may become a doctor much as he has done. Neil is under pressure from his stern father's will. Also, Mr. Perry tells Neil that Mrs. Perry also wants him to become a doctor, which further worries the boy. A little later, Todd tells Neil that he is in a similar situation with his parents involving his older brother who also attended Welton a few years ago, graduated, and attended Yale Law School and became a lawyer and his parents want the exact same thing for him. But Todd does not have the courage to tell his parents that he instead wants to be a writer, not a lawyer.During the first day of classes Todd and Neil experience the various teaching methods which include speeches by the trig teacher, as well as the Latin teacher, and the math teacher who states that "all 20 questions at the end of the first chapter are due tomorrow". In stark contrast to these orthodox teaching methods, the guys see a different side of the school when they attend English class taught by the newly arrived (and liberal-minded) Mr. Keating (Robin Williams), whom they met briefly during the orientation -- Keating tells his class he was also a student at Welton ("Helton" as the students secretly refer to the institution) himself many years ago. Keating enters his class whistling the 1812 Overture, and he first takes the boys out in the hallway to the school's displays cases containing artifacts of the school's sports achievements. He tells them that they all have the potential to become powerful individuals, and they are responsible for what their futures will hold. These two actions show his difference from the other teachers because no other teacher would commit the actions he does. Also, he tells the boys they may call him "Oh Captain, my Captain", if they dare. These examples of Mr. Keating's teachings show the boys how to think for themselves. Mr. Keating then tells the boys "Carpe Diem", which is Latin for "seize the day".In addition to Todd and Neil, a small group of other students whom include the lovesick Knox Overstreet (Josh Charles), the flip Charlie Dalton (Gale Hansen), the pragmatic Richard Cameron (Dylan Kussman), liberal Steven Meeks (Allelon Ruggiero) and the moderate Gerard Pitts (James Waterston), also react to the first day's lesson with comments from "that was weird" to "neat". Cameron asks if anything Keating told them will be on a future test and the boys respond with mild scorn.The next day Keating starts the class with a traditional teaching approach by having Neil read out loud the introduction to their poetry textbook, which describes how to rate the quality of poetry according to mathematical plotting. Keating finds such mathematical criticism ridiculous and instructs his pupils to rip out the essay which is one of three ways that he demonstrates freedom of expression and non-conformity. When some students hesitate, he tells them "this is not the Bible. This is a battle, a war. You will have to learn to think for yourselves." He later has the students stand on his desk as a reminder to look at the world in a different way.A few days later, Knox Overstreet is asked to attend a dinner party at the Danburry 
 Question: Who does Nolan warn?

A: todd anderson
****
Q: Context: Soil is a complex mixture of different materials. About half of most soils are inorganic materials, such as the products of weathered rock, including pebbles, sand, silt, and clay particles. About half of all soils are organic materials, formed from the partial breakdown and decomposition of plants and animals. The organic materials are necessary for a soil to be fertile. The organic portion provides the nutrients, such as nitrogen, needed for strong plant growth. In between the solid pieces, there are tiny spaces filled with air and water. Within the soil layer, important reactions between solid rock, liquid water, air, and living things take place. In some soils, the organic portion could be missing, as in desert sand. Or a soil could be completely organic, such as the materials that make up peat in a bog or swamp (Figure 1.1). The inorganic portion of soil is made of many different size particles, and these different size particles are present in different proportions. The combination of these two factors determines some of the properties of the soil. A permeable soil allows water to flow through it easily because the spaces between the inorganic particles are large and well connected. Sandy or silty soils are considered "light" soils because they are permeable, water-draining types of soils. Soils that have lots of very small spaces are water-holding soils. For example, when clay is present in a soil, the soil is heavier, holds together more tightly, and holds water. When a soil contains a mixture of grain sizes, the soil is called a loam (Figure 1.2). A loam field. When soil scientists want to precisely determine soil type, they measure the percentage of sand, silt, and clay. They plot this information on a triangular diagram, with each size particle at one corner (Figure 1.3). The soil type can then be determined from the location on the diagram. At the top, a soil would be clay; at the left corner, it would be sand; at the right corner, it would be silt. Soils in the lower middle with less than 50% clay are loams. Soil types by particle size. Soil is an ecosystem unto itself. In the spaces of soil, there are thousands or even millions of living organisms. Those organisms could include earthworms, ants, bacteria, or fungi (Figure 1.4). 
 Question: which of these are inorganic materials?

A:
pebbles
****