Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

It is increasingly clear that the normal protein alpha-synuclein is in some manner closely associated with presynaptic components of select neuronal types within the adult human central nervous system (CNS) and, in addition, that in its pathologically altered state alpha-synuclein aggregates selectively in the form of filamentous inclusion bodies during certain progressive neurodegenerative disorders, such as familial and sporadic Parkinson's disease. By having the antibody AFshp raised specifically to alpha-synuclein to label Parkinson disease-specific Lewy bodies and Lewy neurites as well as synaptic boutons containing the unaltered protein, an initial attempt is made to map the overall distribution pattern and describe the staining behavior of the immunoreactive punctae in select regions of the prosencephalon. Neocortical immunolabeling is most prominent in the prodigious, but incompletely myelinated, association fields and faintest in the heavily myelinated primary motor and primary sensory fields, with the premotor and first order sensory association areas occupying an intermediate position. Of the thalamic grays evaluated, those containing powerfully myelinated fiber tracts (e.g. centrum medianum, habenular complex) show the weakest immunolabeling, whereas, less sturdily myelinated structures are highly immunoreactive. The fact that the immunostaining spectrum for normal alpha-synuclein is so broad, together with the fact that some thalamic sites actually are immunonegative leads to the following conclusions (1) alpha-synuclein, although present in the synaptic boutons of many nerve cells in the adult human CNS, is by no means ubiquitous there, and (2) neuronal types lacking the normal protein cannot generate the Parkinson's disease-specific filamentous pathology.
Against which protein is the antibody used for immonostaining of Lewy bodies raised?

If you want to become a fluent English speaker you should take some advice: There are four skills in learning English. They are reading, listening, speaking, and writing. The most important thing you must remember is that if you want to improve your speaking and writing skills you should first master the skills of reading and listening. Read as much as you can. But your reading must be active. It means that you must think about the meaning of the sentence, the meanings of the unfamiliar words, etc. There is no need for you to pay much attention to grammars or try to understand all the unfamiliar words you _ , but the fact that you see them for the first time and recognize them whenever you see them, for example in other passages or books, is enough. It would be better to prepare yourself a notebook so you can write down the important words or sentences in it. As for listening, there are two choices: besides reading, you can listen every day for about 30 minutes. You can only pay attention to your reading and become skillful at your reading, then you can catch up on your listening. Since you have lots of inputs in your mind, you can easily guess what the speaker is going to say. This never means that you should not practice listening. For listening you can listen to cartoons or some movies that are specially made for children. Their languages are easy. Or if you are good at listening you can listen to VOA or BBC programs every day. Again the thing to remember is being active in listening and preferably taking some notes. If you follow these pieces of advice, your speaking and writing will improve automatically, and you can be sure that with a little effort they will become perfect.
According to the author, which should you improve first among the four skills?

Mowat-Wilson syndrome (MWS) is a relatively newly described multiple congenital anomaly/mental retardation syndrome. Haploinsufficiency of a gene termed ZFHX1B (also known as SIP1) on chromosome 2 is responsible for this condition, and clinical genetic testing for MWS recently became available. The majority of reports in the literature originate from Northern Europe and Australia. Here we report our clinical experience with 12 patients diagnosed with MWS within a 2-year period of time in the United States, with particular emphasis on clinical characteristics and management strategies. Individuals with this condition have characteristic facial features, including microcephaly, hypertelorism, medially flared and broad eyebrows, prominent columella, pointed chin, and uplifted earlobes, which typically prompt the clinician to consider the diagnosis. Medical issues in our cohort of patients included seizures (75%) with no predeliction for any particular seizure type; agenesis of the corpus callosum (60% of our patients studied); congenital heart defects (75%), particularly involving the pulmonary arteries and/or valves; hypospadias (55% of males); severely impaired or absent speech (100% of individuals over 1 year of age) with relatively spared receptive language; and Hirschsprung disease (50%) or chronic constipation (25%). The incidence of MWS is unknown, but based on the number of patients identified in a short period of time within the US, it is likely greatly under recognized. MWS should be considered in any individual with severely impaired or absent speech, especially in the presence of seizures and anomalies involving the pulmonary arteries (particularly pulmonary artery sling) or pulmonary valves.

Which gene is responsible for the development of the Mowat-Wilson syndrome?