In this task, you are given a sentence. You are expected to write the name of the organism substances in the body mentioned in the input. Although there might be several correct answers, you need to write one of them.

 At 48 h post - transfection , cell lysates were prepared using 100 mul per well of lysis buffer ( 1 % Triton X - 100 , 1 mm dithiothreitol , 92 . 8 mm K2HPO4 , pH 7 . 8 , 9 . 2 mm KH2PO4 , pH 7 . 8 ) and centrifuged for 5 min at 4 degreesC .
cell lysates

 ( B ) Real - time PCR was performed on total RNA samples from the human brain regions known to express MOR1 .
samples

 A wide range of binding ( 5 . 9 to 130 pmole / ml plasma ) was observed , and significant elevations were found for patients having breast cancer ( 21 . 2 + / - 1 . 9 , P less than 0 . 05 ) and melanoma ( 25 . 7 + / - 1 . 9 , P less than 0 . 001 ) compared to controls ( 15 . 5 + / - 1 . 9 ) .

plasma