Given a passage, construct a question on the basis of the information present in the passage. Construct the question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) it answer uses text phrases from the passage. Avoid creating questions that (i) can't be answered correctly without actually understanding the passage and (ii) uses the same words or phrases given in the passage.

Q: The tumor susceptibility gene 101 (TSG101) was originally identified as a tumor-suppressor gene that mediates many molecular and biological processes, such as ubiquitination, endosomal trafficking, cell survival, and virus budding, but its role in hepatocellular carcinoma (HCC) is currently unknown.', 'We assessed the expression of TSG101 in HCC and paracancerous tissues using qPCR. Then, we used the TSG101-specific siRNA mix to disrupt the expression of TSG101 to investigate the subsequent effect on human hepatoma-7 (Huh7) cells. Western blot was used to detect the protein expression of TSG101 and other molecules. Cell growth assay was performed using CCK8. Transwell assay was used to investigate the migration and invasion ability of Huh7 cells after transfection with of TSG101 siRNA. Flow cytometry was used to estimate the effect of TSG101 knockdown on cell cycle and apoptosis. Confocal laser scanning microscopy was used to observe the actin filaments change and the formation of autophagy.', 'TSG101 was over-expressed in HCC tissues. TSG101 silence was able to suppress Huh7 cell proliferation, migration, and invasion. Furthermore, silencing of TSG101 could induce cell cycle arrest at G1 phase and inhibit the expression of cyclin A and cyclin D, while up-regulating the expression of CDK2. The mechanism might be induction of autophagic cell death and inactivation of Akt and ERK1/2.

A: Does tSG101 Silencing suppress Hepatocellular Carcinoma Cell Growth by Inducing Cell Cycle Arrest and Autophagic Cell Death?
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Q: Few studies investigated the best method for removing stains from different types of resin-based composite restorations and compared them to the more recently introduced nanocomposites. This study compared the effect of four methods for stain removal from composite resins; finishing with Sof-lex disks, using pumice and brush, bleaching with 10% carbamide peroxide and 38% hydrogen peroxide.', 'Twenty disk specimens were prepared. Specimens were immersed in a staining solution for 3\xa0weeks. The stained surfaces of five specimens from each RBC material were treated with one of the treatment procedures. Colorimetric measurements were taken using spectrophotometer prior to and after staining, and then repeated after surface treatments. Color difference values were calculated.', "One-way ANOVA indicated significant differences in color change of the three composite resin materials following staining. Filtek Z250 showed the least susceptibility to discoloration followed by Renamel, Filtek Supreme was the material most prone to discoloration. Two-way ANOVA and Tukey's Post Hoc showed that all stain removing procedures except polishing with pumice, were able to return Filtek Z250 to clinically acceptable color difference. While bleaching with 38% carbamide peroxide was not effective with Renamel. Only pumice and 10% carbamide peroxide were able to return Renamel to clinically acceptable color difference.

A: Do the effectiveness of four methods for stain removal from direct resin-based composite restorative materials?
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Q: To assess the incidence of retained products of conception (RPOC) in relation to transvaginal ultrasound performed after first-trimester uterine evacuation.', 'This was a prospective randomized study involving 809 women undergoing first-trimester uterine evacuation. The study group included 404 women in whom transvaginal sonography was performed at the end of the surgical procedure and the control group contained 405 women who did not undergo ultrasound examination. Initially, in the study group, recurettage was immediately performed if the endometrium appeared irregular but latterly only if endometrial thickness was > or = 8 mm. The patients were followed up by gynecological and ultrasound examinations 5-8 days following the surgical procedure.', 'The total complication rate was 4.3%. RPOC presented in three women in the study group (0.7%) and in 15 women in the control group (3.7%, P < 0.05). Vaginal bleeding requiring hospitalization occurred in two women in the study group (0.5%) vs. seven in the control group (1.7%, P = 0.2). Endometritis was diagnosed in one woman in the study group (0.2%) vs. six in the control group (1.5%) and uterine perforation occurred in one woman in the control group vs. none in the study group. There were no cases of RPOC in women who had an endometrial thickness of < 8 mm as demonstrated by ultrasound at the end of the surgical procedure.

A:
Does transvaginal ultrasound after first-trimester uterine evacuation reduce the incidence of retained products of conception?
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