Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.

Q: Many different cellular pathways have evolved to protect the genome from the deleterious effects of DNA damage that result from exposure to chemical and physical agents. Among these is a process called transcription-coupled repair (TCR) that catalyzes the removal of DNA lesions from the transcribed strand of expressed genes, often resulting in a preferential bias of damage clearance from this strand relative to its non-transcribed counterpart. Lesions subject to this type of repair include cyclobutane pyrimidine dimers that are normally repaired by nucleotide excision repair (NER) and thymine glycols (TGs) that are removed primarily by base excision repair (BER). While the mechanism underlying TCR is not completely clear, it is known that its facilitation requires proteins used by other repair pathways like NER. It is also believed that the signal for TCR is the stalled RNA polymerase that results when DNA damage prevents its translocation during transcription elongation. While there is a clear role for some NER proteins in TCR, the involvement of BER proteins is less clear. To explore this further, we studied the removal of 7-methylguanine (7MeG) and 3-methyladenine (3MeA) from the dihydrofolate reductase (dhfr) gene of murine cell lines that vary in their repair phenotypes. 7MeG and 3MeA constitute the two principal N-methylpurines formed in DNA following exposure to methylating agents. In mammalian cells, alkyladenine DNA alkyladenine glycosylase (Aag) is the major enzyme required for the repair of these lesions via BER, and their removal from the total genome is quite rapid. There is no observable TCR of these lesions in specific genes in DNA repair proficient cells; however, it is possible that the rapid repair of these adducts by BER masks any TCR. The repair of 3MeA and 7MeG was examined in cells lacking Aag, NER, or both Aag and NER to determine if rapid overall repair masks TCR. The results show that both 3MeA and 7MeG are removed without strand bias from the dhfr gene of BER deficient (Aag deficient) and NER deficient murine cell lines. Furthermore, repair of 3MeA in this region is highly dependent on Aag, but repair of 7MeG is equally efficient in the repair proficient, BER deficient, and NER deficient cell lines. Strikingly, in the absence of both BER and NER, neither 7MeG nor 3MeA is repaired. These results demonstrate that NER, but not TCR, contributes to the repair of 7MeG, and to a lesser extent 3MeA.

A: Which gene strand is targeted by transcription-coupled repair (TCR)?
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Q: You must have been troubled by when to say "I love you" because it is one of the greatest puzzles in our life. What if you say it first and your partner doesn't love you back? Or if they do say it but you don't feel they mean it? Being the first to declare your love can be never racking and risky and can leave you feeling as vulnerable as a turtle with no shell. But is the person who says it first really in a position of weakness? Doesn't it pay to hold back, play it cool and wait until the other half has shown their hand faster? "A really good relationship should be about being fair and being equal," says psychologist Sidney Crown. "But love is seldom equal." "All relationships go through power struggles but," he says, "if a love imbalance continues for years, the rot will set in." That feeling of "I've always loved you more" may be subverted (,) for a time, but it never goes away completely and it often emerges in squabbling . In love, at least, the silent, withholding type is not always the most powerful. "The strongest one in a relationship is often the person who feels confident enough to talk about their feelings," says educational psychologist Ingrid Collins. Psychosexual therapist Paula Hall agrees. "The one with the upper hand is often the person who takes the initiative." In fact, the person who says "I love you" first may also be the one who says "I'm bored with you' first." Hall believes that much depends on how "I love you" is said and the motivation of the person saying it. Is it said when they're drunk? Is it said before their partner flies off on holiday, and what it really means is "Please don' t be unfaithful to me" ? By saying "I love you", they are really saying "Do you love me?" If so, wouldn't it just be more honest to say that. Collins agrees that intention is everything. "It's not what is said, but how it's said. What it comes down to is the sincerity of the speaker."

A: What is the most important for you to consider when somebody say "I love you" to you?
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Q: Lewy bodies and coarse Lewy neurites are the pathological hallmarks of degenerating neurons in the brains of patients suffering from Parkinson's disease (PD). Recently, the presynaptic protein alpha-synuclein was shown to be a major component of Lewy bodies and Lewy neurites. This study demonstrates for the first time that extensive and thin alpha-synuclein-immunoreactive inclusions are present in the axonal processes of neurons.

A:
Which is the primary protein component of Lewy bodies?
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