instruction:
In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
question:
We evaluated the safety and immunogenicity of a chimeric alphavirus vaccine candidate in mice with selective immunodeficiencies. This vaccine candidate was highly attenuated in mice with deficiencies in the B and T cell compartments, as well as in mice with deficient gamma-interferon responsiveness. However, the level of protection varied among the strains tested. Wild type mice were protected against lethal VEEV challenge. In contrast, alpha/beta (αβ) TCR-deficient mice developed lethal encephalitis following VEEV challenge, while mice deficient in gamma/delta ( γδ) T cells were protected. Surprisingly, the vaccine potency was diminished by 50% in animals lacking interferon-gamma receptor alpha chain (R1)-chain and a minority of vaccinated immunoglobulin heavy chain-deficient (μMT) mice survived challenge, which suggests that neutralizing antibody may not be absolutely required for protection. Prolonged replication of encephalitic VEEV in the brain of pre-immunized mice is not lethal and adoptive transfer experiments indicate that CD3 + T cells are required for protection.
answer:
Alpha-beta T cells provide protection against lethal encephalitis in the murine model of VEEV infection


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Bovine renal brush-border membrane vesicle aminopeptidase N at various stages of purity was treated with two bifunctional cross-linking agents. A pattern of emergence of higher molecular weight forms was observed. By using a cleavable cross-linker, aminopeptidase N was shown to cross-link both to itself and to its breakdown products as well as to dipeptidyl peptidase IV. Using this technique it was possible to identify three of the breakdown products as 45 kDa, 66 kDa and 95 kDa peptides. N-terminal amino acid sequence analysis was used to define the precise cleavage points for the bovine renal aminopeptidase N breakdown products. The short amino acid sequences obtained show strong sequence similarity with the human intestinal and rat kidney aminopeptidase N.
answer:
The oligomeric structure of renal aminopeptidase N from bovine brush-border membrane vesicles


question:
Transmissible gastroenteritis virus was administered orally to cats. No clinical disease resulted but infectious virus was isolated from faeces for up to 22 days after infection and serum antibody was detected by neutralisation and immunofluorescence tests. . The spread of transmissible gastroenteritis virus (TGEV) from infected pig herds and the recrudescence of infection after long periods have not been adequately explained. Experiments on the host range of the virus have indicated that nonporcine hosts could have a role in the epidemiology of TGE (2, 4, 7, 8, 9, t6). Indirect immunofluorescence (IIF) and virus neutralisation (V1N!) tests have been used recently to demonstrate antibody to TGEV in cats. In Holland and Great Britain samples from cats with clinical feline infectious peritonitis (FIP) frequently have antibody to TGEV in the I I F test (10). However, neutralising antibody has not been found in sera from Dutch cats with FIP. American and Dutch studies have recently demonstrated that sera from cats experimentally infected with F I P will cross-react, at low titre, with TGEV-infected pig cells by immunofluoreseence but will not neutralise TGEV in vitro (10, 12) . This evidence has been used to suggest that an antigenic relationship exists between TGEV and F I P virus. The potential of the cat to excrete TGEV was studied by virus reisolation and the serological responses to TGEV and F I P were monitored; thus the possible epidemiological role of the cat in TGE could be assessed. In addition information was obtained on the possible origin and relationship of VN and I I F antibodies to TGEV which occur naturally in cats, often at high titres (10, 13). TGEV strain KN63, used as inoculum in this experiment, was prepared as a clarified 50 per cent suspension of small intestine from an infected gnotobiotic piglet. I t had a titre of i.7 × 107 PFU/ml.
answer:

Archives of Virology Virus Isolation and Serum, Antibody Responses After Infection of Cats With Transmissible Gastroenteritis Virus BrieiReport