instruction:
Given a context passage, generate a question from the passage such that its answer is shortest continous span from the passage.
question:
Lindsay (Ashley C. Williams) and Jenny (Ashlynn Yennie), two American tourists in Germany, are drugged and involuntarily detained by crazed surgeon Dr. Josef Heiter (Dieter Laser) when they seek help at his house after they get a flat tire. The women awake in a makeshift medical ward. They witness Heiter kill a kidnapped truck driver after Heiter informs him he is "not a match". Heiter secures a new male captive, Japanese tourist Katsuro (Akihiro Kitamura). The doctor explains that he is a world-renowned expert at separating Siamese twins, but dreams of making new creatures by sewing people together. He describes in detail how he will surgically connect his three victims mouth-to-anus, so that they share a single digestive system. After Lindsay tries to escape and fails, Heiter explains that he had previously experimented with creating what he called a "three-dog", also joined mouth-to-anus. However, the three-dog died shortly after surgery. Heiter tells Lindsay that one dog tried to escape and that dog became the middle, thus this caused the most pain to the dog and as punishment for her escape attempt, she will become the middle part of his centipede. Heiter performs the surgery, placing Katsuro at the front, Lindsay in the middle and Jenny at the rear. He removes the front teeth of both women and mutilates the buttocks of both Katsuro and Lindsay in order to provide easier access to the rectums, to which he hideously stitches and grafts their mouths. During his procedure, he severs the ligaments within his victims' knees to prevent leg extension, forcing his victims to crawl. He then connects his victims together in a straight line with their mouths surgically attached to the anus of the victim in front of them. Once the operation is complete, Heiter attempts to train his centipede as a pet, often belittling Katsuro with racist insults and beating him with a crop when he becomes rebellious. When Katsuro defecates, Lindsay is forced to swallow his excrement while the doctor watches in delight. However, he eventually becomes irritated after being kept awake by the constant screaming of Katsuro (who, as the front part of the centipede, has his mouth free and is still able to speak) and the realization that Jenny is dying from septicemia. When two detectives, Kranz (Andreas Leupold) and Voller (Peter Blankenstein), visit the house to investigate the disappearance of tourists, Heiter comes up with an idea to add them as replacements for Jenny in a new creation; a four-segment centipede. He offers the two detectives water spiked with sedatives in hopes of knocking the two unconscious. After being given the water, the detectives become suspicious and obtain a search warrant for his home. While the detectives leave Heiter's home, the victims attempt to escape. Katsuro attacks Heiter in the process. Their attempt to escape ultimately fails. Katsuro confesses to the doctor that he deserves his fate because he had treated his own family poorly, then commits suicide by slitting his throat with a glass shard. Upon returning to Heiter's home, the detectives forcefully conduct separate searches as an injured Heiter hides near his swimming pool. Kranz finds the ward along with Heiter's victims. Voller begins to feel ill from the earlier drugging, and Heiter stabs him with the scalpel pulled from his leg during Katsuro's attack. Upon finding Voller dead, Kranz is shot by Heiter with Voller's sidearm. Kranz responds by shooting Heiter in the head before dying. Back in the house, Jenny and Lindsay hold hands as Jenny dies. Lindsay sobs as she is left alone in the house, trapped between her deceased fellow captives. Her fate is left unknown. The film ends with the sounds of her sobbing while the camera pans to the roof of the house.
answer:
what type of poisoning is Jenny dying from?


question:
In eukaryotic cells, there are two sub-pathways of nucleotide excision repair (NER), the global genome (gg) NER and the transcription-coupled repair (TCR). TCR can preferentially remove the bulky DNA lesions located at the transcribed strand of a transcriptional active gene more rapidly than those at the untranscribed strand or overall genomic DNA. This strand-specific repair in a suitable restriction fragment is usually determined by alkaline gel electrophoresis followed by Southern blotting transfer and hybridization with an indirect end-labeled single-stranded probe. Here we describe a new method of TCR assay based on strand-specific-PCR (SS-PCR). Using this method, we have investigated the role of DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase-related protein kinases (PIKK) family, in the TCR pathway of UV-induced DNA damage. Although depletion of DNA-PKcs sensitized HeLa cells to UV radiation, it did not affect the ggNER efficiency of UV-induced cyclobutane pyrimidine dimers (CPD) damage. We postulated that DNA-PKcs may involve in the TCR process. To test this hypothesis, we have firstly developed a novel method of TCR assay based on the strand-specific PCR technology with a set of smart primers, which allows the strand-specific amplification of a restricted gene fragment of UV radiation-damaged genomic DNA in mammalian cells. Using this new method, we confirmed that siRNA-mediated downregulation of Cockayne syndrome B resulted in a deficiency of TCR of the UV-damaged dihydrofolate reductase (DHFR) gene. In addition, DMSO-induced silencing of the c-myc gene led to a decreased TCR efficiency of UV radiation-damaged c-myc gene in HL60 cells. On the basis of the above methodology verification, we found that the depletion of DNA-PKcs mediated by siRNA significantly decreased the TCR capacity of repairing the UV-induced CPDs damage in DHFR gene in HeLa cells, indicating that DNA-PKcs may also be involved in the TCR pathway of DNA damage repair. By means of immunoprecipitation and MALDI-TOF-Mass spectrometric analysis, we have revealed the interaction of DNA-PKcs and cyclin T2, which is a subunit of the human transcription elongation factor (P-TEFb). While the P-TEFb complex can phosphorylate the serine 2 of the carboxyl-terminal domain (CTD) of RNA polymerase II and promote transcription elongation. A new method of TCR assay was developed based the strand-specific-PCR (SS-PCR). Our data suggest that DNA-PKcs plays a role in the TCR pathway of UV-damaged DNA. One possible mechanistic hypothesis is that DNA-PKcs may function through associating with CyclinT2/CDK9 (P-TEFb) to modulate the activity of RNA Pol II, which has already been identified as a key molecule recognizing and initializing TCR.
answer:
Which gene strand is targeted by transcription-coupled repair (TCR)?


question:
Khivi (1506--1582) also referred to as Mata Khivi or Bibi Khivi was the wife of Guru Angad Dev, second Sikh guru.
answer:

What is Khivi's spouse's name?