Given a question and a context passage, generate the answer having the word or phrase from the context passage. Here, the answer should be a shortest continous span from the passage.

Ex Input:
Context: In eukaryotic cells, there are two sub-pathways of nucleotide excision repair (NER), the global genome (gg) NER and the transcription-coupled repair (TCR). TCR can preferentially remove the bulky DNA lesions located at the transcribed strand of a transcriptional active gene more rapidly than those at the untranscribed strand or overall genomic DNA. This strand-specific repair in a suitable restriction fragment is usually determined by alkaline gel electrophoresis followed by Southern blotting transfer and hybridization with an indirect end-labeled single-stranded probe. Here we describe a new method of TCR assay based on strand-specific-PCR (SS-PCR). Using this method, we have investigated the role of DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase-related protein kinases (PIKK) family, in the TCR pathway of UV-induced DNA damage. Although depletion of DNA-PKcs sensitized HeLa cells to UV radiation, it did not affect the ggNER efficiency of UV-induced cyclobutane pyrimidine dimers (CPD) damage. We postulated that DNA-PKcs may involve in the TCR process. To test this hypothesis, we have firstly developed a novel method of TCR assay based on the strand-specific PCR technology with a set of smart primers, which allows the strand-specific amplification of a restricted gene fragment of UV radiation-damaged genomic DNA in mammalian cells. Using this new method, we confirmed that siRNA-mediated downregulation of Cockayne syndrome B resulted in a deficiency of TCR of the UV-damaged dihydrofolate reductase (DHFR) gene. In addition, DMSO-induced silencing of the c-myc gene led to a decreased TCR efficiency of UV radiation-damaged c-myc gene in HL60 cells. On the basis of the above methodology verification, we found that the depletion of DNA-PKcs mediated by siRNA significantly decreased the TCR capacity of repairing the UV-induced CPDs damage in DHFR gene in HeLa cells, indicating that DNA-PKcs may also be involved in the TCR pathway of DNA damage repair. By means of immunoprecipitation and MALDI-TOF-Mass spectrometric analysis, we have revealed the interaction of DNA-PKcs and cyclin T2, which is a subunit of the human transcription elongation factor (P-TEFb). While the P-TEFb complex can phosphorylate the serine 2 of the carboxyl-terminal domain (CTD) of RNA polymerase II and promote transcription elongation. A new method of TCR assay was developed based the strand-specific-PCR (SS-PCR). Our data suggest that DNA-PKcs plays a role in the TCR pathway of UV-damaged DNA. One possible mechanistic hypothesis is that DNA-PKcs may function through associating with CyclinT2/CDK9 (P-TEFb) to modulate the activity of RNA Pol II, which has already been identified as a key molecule recognizing and initializing TCR. 
 Question: Which gene strand is targeted by transcription-coupled repair (TCR)?

Ex Output:
the transcribed strand


Ex Input:
Context: The smallest planet, Mercury, is the planet closest to the Sun. Because Mercury is so close to the Sun, it is difficult to observe from Earth, even with a telescope. However, the Mariner 10 spacecraft, shown in Figure 1.1, visited Mercury from 1974 to 1975. The MESSENGER spacecraft has been studying Mercury in detail since 2005. The craft is currently in orbit around the planet, where it is creating detailed maps. MESSENGER stands for Mercury Surface, Space Environment, Geochemistry and Ranging. (a) Mariner 10 made three flybys of Mercury in 1974 and 1975. (b) A 2008 image of compiled from a flyby by MESSENGER. As Figure 1.2 shows, the surface of Mercury is covered with craters, like Earths Moon. Ancient impact craters means that for billions of years Mercury hasnt changed much geologically. Also, with very little atmosphere, the processes of weathering and erosion do not wear down structures on the planet. Mercury is named for the Roman messenger god, who could run extremely quickly, just as the planet moves very quickly in its orbit around the Sun. A year on Mercury the length of time it takes to orbit the Sun is just 88 Earth days. Despite its very short years, Mercury has very long days. A day is defined as the time it takes a planet to turn on its axis. Mercury rotates slowly on its axis, turning exactly three times for every two times it orbits the Sun. Therefore, each day on Mercury is 57 Earth days long. In other words, on Mercury, a year is only a Mercury day and a half long! Mercury is close to the Sun, so it can get very hot. However, Mercury has virtually no atmosphere, no water to insulate the surface, and it rotates very slowly. For these reasons, temperatures on the surface of Mercury vary widely. In direct sunlight, the surface can be as hot as 427 C (801 F). On the dark side, or in the shadows inside craters, the surface can be as cold as -183 C (-297 F)! Although most of Mercury is extremely dry, scientists think Mercury is covered with craters, like Earths Moon. MESSENGER has taken extremely detailed pictures of the planets surface. there may be a small amount of water in the form of ice at the poles of Mercury, in areas that never receive direct sunlight. Figure 1.3 shows a diagram of Mercurys interior. Mercury is one of the densest planets. Its relatively large, liquid core, made mostly of melted iron, takes up about 42% of the planets volume. 
 Question: the core of mercury is made mostly of melted

Ex Output:
iron


Ex Input:
Context: Restless legs syndrome (RLS)/Willis-Ekbom disease (WED) is a common disorder, occurring at least twice a week and causing at least moderate distress in 1.5% to 2.7% of the population. It is important for primary care physicians to be familiar with this disorder and its management. Much has changed in its management since our previous algorithm was published in 2004, including the availability of several new drugs. This revised algorithm was written by members of the Medical Advisory Board of the Willis-Ekbom Disease Syndrome Foundation based on scientific evidence and expert opinion. It considers the management of RLS/WED under intermittent RLS/WED, chronic persistent RLS/WED, and refractory RLS/WED. Nonpharmacological approaches, including mental alerting activities, avoiding substances or medications that may exacerbate RLS, and the role of iron supplementation, are outlined. Chronic persistent RLS/WED should be treated with either a nonergot dopamine agonist or a calcium channel -2- ligand. We discuss the available drugs, the factors determining which to use, and their adverse effects. We define refractory RLS/WED and describe management approaches, including combination therapy and the use of high-potency opioids. 
 Question: Willis-Ekbom disease is also known as?

Ex Output:

restless legs syndrome