In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

[Q]: Ranavirus cross-species infections have been documented, but the viral proteins involved in the interaction with cell receptors have not yet been identified. Here, viral cell-binding proteins and their cognate cellular receptors were investigated using two ranaviruses, Andrias davidianus ranavirus (ADRV) and Rana grylio virus (RGV), and two different cell lines, Chinese giant salamander thymus cells (GSTC) and Epithelioma papulosum cyprinid (EPC) cells. The heparan sulfate (HS) analog heparin inhibited plaque formation of ADRV and RGV in the two cell lines by more than 80% at a concentration of 5 µg/mL. In addition, enzymatic removal of cell surface HS by heparinase I markedly reduced plaque formation by both viruses and competition with heparin reduced virus-cell binding. These results indicate that cell surface HS is involved in ADRV and RGV cell binding and infection. Furthermore, recombinant viral envelope proteins ADRV-58L and RGV-53R bound heparin-Sepharose beads implying the potential that cell surface HS is involved in the initial interaction between ranaviruses and susceptible host cells. To our knowledge, this is the first report identifying cell surface HS as ranavirus binding factor and furthers understanding of interactions between ranaviruses and host cells.
[A]: Ranaviruses Bind Cells from Different Species through Interaction with Heparan Sulfate


[Q]: Streptococcus pneumoniae is the main cause of diseases such as meningitis, pneumoniae and sepsis, especially in children and old people. Due to costly antibiotic treatment, and increasing resistance of pneumococcus, developing high-efficient protective vaccine against this pathogen is an urgent need. Although the pneumoniae polysaccharide vaccine (PPV) and pneumonia conjugate vaccines (PCV) are the efficient pneumococcal vaccine in children and adult groups, but the serotype replacement of S. pneumoniae strains causes the reduction in efficacy of such vaccines. For overcoming the aforesaid drawbacks epitope-based vaccines are introduced as the relevant alternative. In our previous research, the epitope vaccine was designed based on immunodominant epitopes from PspA, CbpA antigens as cellular stimulants and PhtD, PiuA as humoral stimulants. Because the low immunogenicity is the main disadvantage of epitope vaccine, in the current study, we applied coiled-coil selfassembled structures for developing our vaccine. Recently, self-assembled peptide nanoparticles (SAPNs) have gained much attention in the field of vaccine development due to their multivalency, self-adjuvanticity, biocompatibility, and size similarity to pathogen. In this regard, the final designed vaccine is comprised of cytotoxic T lymphocytes (CTL) epitopes from PspA and CbpA, helper T lymphocytes (HTL) epitopes from PhtD and PiuA, the pentamer and trimmer oligomeric domains form 5-stranded and 3-stranded coiled-coils as self-assembled scaffold, Diphtheria toxoids (DTD) as a universal T-helper, which fused to each other with appropriate linkers. The four different arrangements based on the order of above-mentioned compartments were constructed, and each of them were modeled, and validated to find the 3D structure. The structural, physicochemical, and immunoinformatics analyses of final vaccine construct represented that our vaccine could stimulate potent immune response against S. pneumoniae; however, the potency of that should be approved via various in vivo and in vitro immunological tests.
[A]: Designing self-assembled peptide nanovaccine against Streptococcus pneumoniae: An in silico strategy


[Q]: Breathing is a high-risk behavior for spreading infectious diseases in enclosed environments, so it is important to investigate the characteristics of human exhalation flow and dispersal of exhaled air to reduce the risk. This paper used two-dimensional time-resolved particle image velocimetry (2D TR-PIV) to measure the exhaled flow from a breathing thermal manikin. Since the exhaled flow is transient and periodic, the phase-averaged method was used to analyze the flow characteristics. The results showed that the velocity profile of the flow exiting the mouth was bell-shaped for exhalation and flat for inhalation. The exhaled flow showed different characteristics during each stage of the exhalation process. In the initial phase, a mushroom-shaped flow arose, while some jet characteristics appeared in the middle phase. The effect of thermal buoyancy and thermal plume on the exhaled flow was analyzed. Clear turbulence characteristics were found in the exhaled flow, and the turbulence fluctuation was very strong in the transition stage between exhalation and inhalation. The last finding was that the distribution and value of vorticity were different in each phase. The results of quantitative PIV provided detailed information about the boundary condition set and validation data for CFD simulation.
[A]:
TR-PIV measurement of exhaled flow using a breathing thermal manikin