2010 Australian Open Singles seeds Women's singles

| Sd | Player | Points | Points_defending | Points_won | New_points | Status_ |
|---|---|---|---|---|---|---|
| 25 | Anabel Medina Garrigues | 1980 | 280 | 5 | 1705 | 1st round lost to Karolina Šprem |
| 26 | Aravane Rezaï | 2055 | 5 | 100 | 2150 | 2nd round lost to Angelique Kerber [Q] |
| 27 | Alisa Kleybanova | 1870 | 280 | 160 | 1750 | 3rd round lost Justine Henin [WC] |
| 28 | Elena Vesnina | 1900 | 5 | 5 | 1900 | 1st round lost to Tathiana Garbin |
| 29 | Shahar Pe`er | 1930 | 5 | 160 | 2085 | 3rd round lost to Caroline Wozniacki [4] |
| 30 | Kateryna Bondarenko | 1740 | 160 | 100 | 1680 | 2nd round lost to Elena Baltacha |
| 31 | Alyona Bondarenko | 1900 | 160 | 280 | 2020 | 4th round lost to Zheng Jie |