TranSouth Athletic Conference Former members Former members

| Institution | Location_ | Founded | Type_ | Enrollment | Nickname | Joined | Left_ | Current_Conference |
|---|---|---|---|---|---|---|---|---|
| Berry College | Mount Berry, Georgia | 1902 | Private | 1937 | Vikings | 1996 | 2004 | SAA (NCAA Division III) |
| Birmingham–Southern College | Birmingham, Alabama | 1856 | Private | 1400 | Panthers | 1996 | 2001 | SAA (NCAA Division III) |
| Lipscomb University | Nashville, Tennessee | 1891 | Private | 4278 | Bisons | 1996 | 2001 | Atlantic Sun (A-Sun) (NCAA Division I) |
| Lee University | Cleveland, Tennessee | 1918 | Private | 4954 | Flames | 1996 | 2004 | SSAC , Gulf South in 2013. |
| Trevecca Nazarene University | Nashville, Tennessee | 1901 | Private | 2345 | Trojans | 1996 | 2012 | G-MAC (NCAA Division II) |
| Union University | Jackson, Tennessee | 1823 | Private | 4259 | Union | 1996 | 2012 | Gulf South (GSC) (NCAA Division II) |