1990 NCAA Women's Division I Basketball Tournament First and second rounds First and second rounds

| Region | Rnd | Host_ | Venue | City | State_ |
|---|---|---|---|---|---|
| Mideast | 1 | University of South Carolina | Carolina Coliseum | Columbia | South Carolina |
| Mideast | 1 | Tennessee Tech | Eblen Center | Cookeville | Tennessee |
| Mideast | 1 | Vanderbilt University | Memorial Gymnasium (Vanderbilt University) | Nashville | Tennessee |
| Mideast | 2 | Auburn University | Memorial Coliseum (Beard–Eaves–Memorial Coliseum) | Auburn | Alabama |
| Mideast | 2 | Northwestern University | Welsh-Ryan Arena | Evanston | Illinois |
| Mideast | 2 | University of Iowa | Carver–Hawkeye Arena | Iowa City | Indiana |
| Mideast | 2 | University of Washington | Hec Edmundson Pavilion | Seattle | Washington |
| Midwest | 1 | Northern Illinois University | Chick Evans Field House | DeKalb | Illinois |
| Midwest | 1 | Ohio State University | St. John Arena | Columbus | Ohio |
| Midwest | 1 | University of Southern Mississippi | Reed Green Coliseum | Hattiesburg | Mississippi |
| Midwest | 1 | Oklahoma State University | Gallagher-Iba Arena | Stillwater | Oklahoma |