2008–09 Isthmian League Quarterfinals Quarterfinals

| Tie_no | Home_team | Score | Away_team | Attendance |
|---|---|---|---|---|
| 59 | Billericay Town | 3–2 | Dover Athletic | 201 |
| 60 | Carshalton Athletic | 2–3 | Ashford Town (Middx) | 81 |
| 61 | Harrow Borough | 3–1 | Horsham | 55 |
| 62 | Tilbury | 1–1 † | Staines Town | 41 |
| Tilbury advance 4–2 on penalties | Tilbury advance 4–2 on penalties | Tilbury advance 4–2 on penalties | Tilbury advance 4–2 on penalties | Tilbury advance 4–2 on penalties |