2000–01 FA Cup Fifth round proper Fifth round proper

| Tie_no | Home_team | Score | Away_team | Attendance |
|---|---|---|---|---|
| 1 | Liverpool | 4 – 2 | Manchester City | 36231 |
| 2 | Southampton | 0 – 0 | Tranmere Rovers | 15232 |
| replay | Tranmere Rovers | 4 – 3 | Southampton | 12910 |
| 3 | Leicester City | 3 – 0 | Bristol City | 20905 |
| 4 | Bolton Wanderers | 1 – 1 | Blackburn Rovers | 22048 |
| replay | Blackburn Rovers | 3 – 0 | Bolton Wanderers | 20318 |
| 5 | Sunderland | 0 – 1 | West Ham United | 36005 |
| 6 | Tottenham Hotspur | 4 – 0 | Stockport County | 36040 |
| 7 | Wycombe Wanderers | 2 – 2 | Wimbledon | 9650 |
| replay | Wimbledon | 2 – 2 | Wycombe Wanderers | 9464 |