Mississippi Valley Conference (Wisconsin) Schools Schools

| Institution | Location_ | Year_Joined | Affiliation | Enrollment | Mascot | Colors |
|---|---|---|---|---|---|---|
| Aquinas | La Crosse, WI | 1997 | Private (Catholic) | 330 | Blugolds | Blue and Gold |
| Holmen | Holmen, WI | 1989 | Public | 1077 | Vikings | Maroon and White |
| Central | La Crosse, WI | 1989 | Public | 1316 | Red Raiders | Red and Black |
| Logan | La Crosse, WI | 1989 | Public | 1037 | Rangers | Red and White |
| Onalaska | Onalaska, WI | 1989 | Public | 907 | Hilltoppers | Purple and White |
| Sparta | Sparta, WI | 1989 | Public | 745 | Spartans | Red and Gold |