1991 NCAA Women's Division I Basketball Tournament First and second rounds First and second rounds

| Region | Host_ | Venue | City | State_ |
|---|---|---|---|---|
| Mideast | Southwest Missouri State University | Hammons Student Center | Springfield | Missouri |
| Mideast | Holy Cross | Hart Center | Worcester | Massachusetts |
| Mideast | Purdue University | Mackey Arena | West Lafayette | Indiana |
| Mideast | Western Kentucky University | E.A. Diddle Arena | Bowling Green | Kentucky |
| Mideast | University of Tennessee | Thompson-Boling Arena | Knoxville | Tennessee |
| Mideast | Auburn University | Memorial Coliseum (Beard–Eaves–Memorial Coliseum) | Auburn | Alabama |
| Midwest | Northwestern University | Welsh-Ryan Arena | Evanston | Illinois |
| Midwest | Oklahoma State University | Gallagher-Iba Arena | Stillwater | Oklahoma |
| Midwest | Stephen F. Austin University | William R. Johnson Coliseum | Nacogdoches | Texas |
| Midwest | University of Texas | Frank Erwin Center | Austin | Texas |
| Midwest | Oklahoma State University | Gallagher-Iba Arena | Stillwater | Oklahoma |
| Midwest | University of Virginia | University Hall (University of Virginia) | Charlottesville | Virginia |