2008–09 Copa del Rey Round of 32 Round of 32

| Team_1 | Agg_ | Team_2 | _1st_leg | _2nd_leg_ |
|---|---|---|---|---|
| Real Unión | (a) 6–6 | Real Madrid | 3–2 | 3–4 |
| Portugalete | 1–7 | Valencia | 1–4 | 0–3 |
| Ponferradina | 1–4 | Sevilla | 1–0 | 0–4 |
| Orihuela | 0–1 | Atlético | 0–1 | 0–0 |
| Poli Ejido | 6–1 | Villarreal | 5–0 | 1–1 |
| Hércules | 3–7 | Valladolid | 1–5 | 2–2 |
| Rayo Vallecano | 1–5 | Almería | 1–2 | 0–3 |
| Celta | 2–5 | Espanyol | 2–2 | 0–3 |
| Castellón | 0–4 | Betis | 0–2 | 0–2 |
| Real Murcia | 2–3 | Racing | 2–1 | 0–2 |
| Málaga | 1–3 | Mallorca | 1–1 | 0–2 |
| Athletic | 3–2 | Recreativo | 2–0 | 1–2 |
| Getafe | 0–1 | Osasuna | 0–0 | 0–1 |
| Benidorm | 0–2 | Barcelona | 0–1 | 0–1 |
| Elche | 0–4 | Deportivo | 0–2 | 0–2 |
| Numancia | 0–3 | Sporting | 0–1 | 0–2 |