2010 French Open Singles seeds Women's singles

| Seed | Rank_ | Player | Points | Points_defending | Points_won | New_points | Status_ |
|---|---|---|---|---|---|---|---|
| 24 | 25 | Lucie Šafářová | 2075 | 100 | 100 | 2075 | 2nd round lost to Polona Hercog |
| 25 | 26 | Zheng Jie | 2325 | 100 | 100 | 2325 | 2nd round lost to Anastasia Pivovarova [Q] |
| 26 | 27 | Dominika Cibulková | 2005 | 900 | 160 | 1265 | 3rd round lost to Venus Williams [2] |
| 27 | 28 | Alona Bondarenko | 1700 | 5 | 160 | 1855 | 3rd round lost to Jelena Janković [4] |
| 28 | 29 | Alisa Kleybanova | 1855 | 5 | 160 | 2010 | 3rd round lost to Yaroslava Shvedova |
| 29 | 30 | Anastasia Pavlyuchenkova | 1850 | 160 | 160 | 1850 | 3rd round lost to Serena Williams [1] |
| 30 | 31 | Maria Kirilenko | 1710 | 5 | 280 | 1985 | 4th round lost to Francesca Schiavone [17] |
| 31 | 32 | Alexandra Dulgheru | 1655 | 0(30) | 160 | 1785 | 3rd round lost to Caroline Wozniacki [3] |