2000–01 FA Cup Fourth round proper Fourth round proper

| Tie_no | Home_team | Score | Away_team | Attendance |
|---|---|---|---|---|
| 1 | Bristol City | 1 – 1 | Kingstonian | 14787 |
| replay | Kingstonian | 0 – 1 | Bristol City | 3341 |
| 2 | Southampton | 3 – 1 | Sheffield Wednesday | 15251 |
| 3 | Gillingham | 2 – 4 | Chelsea | 10419 |
| 4 | Blackburn Rovers | 0 – 0 | Derby County | 18858 |
| replay | Derby County | 2 – 5 | Blackburn Rovers | 15203 |
| 5 | Aston Villa | 1 – 2 | Leicester City | 26383 |
| 6 | Bolton Wanderers | 5 – 1 | Scunthorpe United | 11737 |
| 7 | Crewe Alexandra | 0 – 1 | Stockport County | 7318 |
| 8 | Middlesbrough | 0 – 0 | Wimbledon | 20625 |
| replay | Wimbledon | 3 – 1 | Middlesbrough | 5991 |
| 9 | Sunderland | 1 – 0 | Ipswich Town | 33626 |