Swimming at the 2000 Summer Olympics – Women's 100 metre freestyle Semifinal 2 Semifinal 2

| Rank_ | Lane | Name_ | Nationality | Time_ |
|---|---|---|---|---|
| 1 | 4 | Inge de Bruijn | Netherlands | 53.77 |
| 2 | 5 | Jenny Thompson | United States | 54.40 |
| 3 | 3 | Helene Muller | South Africa | 55.24 |
| 4 | 2 | Sumika Minamoto | Japan | 55.62 |
| 5 | 1 | Karen Pickering | Great Britain | 55.71 |
| 6 | 8 | Rania Elwani | Egypt | 55.85 |
| 7 | 7 | Louise Jöhncke | Sweden | 55.94 |
| 8 | 6 | Sandra Völker | Germany | 55.97 |