2000 Copa do Brasil Round of Sixteen Round of Sixteen

| Team_1 | Agg_ | Team_2 | _1st_leg | _2nd_leg |
|---|---|---|---|---|
| Botafogo (RJ) | 3-1 | Corinthians (SP) | 1-0 | 3-1 |
| Cruzeiro (MG) | 4-3 | Atlético (PR) | 2-1 | 2-2 |
| Bahia (BA) | 2-4 | Flamengo (RJ) | 1-3 | 1-1 |
| Juventude (RS) | 1-6 | Santos (SP) | 1-3 | 0-3 |
| ABC (RN) | 4-4 | Palmeiras (SP) | 3-3 | 1-1 |
| América (RN) | 3-6 | São Paulo (SP) | 1-3 | 2-3 |
| Fluminense (RJ) | 3-3 | Vasco (RJ) | 1-1 | 2-3 |
| Portuguesa (SP) | 0-0 | Atlético (MG) | 0-0 | 0-0 (pen: 1-4 |