DNA sequencer Comparison Comparison

| Sequencer | Ion_Torrent_PGM | _454_GS_FLX | HiSeq_2000 | SOLiDv4 | PacBio | Sanger_3730xl |
|---|---|---|---|---|---|---|
| Manufacturer | Ion Torrent (Life Technologies) | 454 Life Sciences (Roche) | Illumina | Applied Biosystems (Life Technologies) | Pacific Biosciences | Applied Biosystems (Life Technologies) |
| Sequencing Chemistry | Ion semiconductor sequencing | Pyrosequencing | Polymerase-based sequence-by-synthesis | Ligation-based sequencing | Phospholinked fluorescent nucleotides | Dideoxy chain termination |
| Amplification approach | Emulsion PCR | Emulsion PCR | Bridge amplification | Emulsion PCR | Single-molecule; no amplification | PCR |
| Data output per run | 100-200 Mb | 0.7 Gb | 600 Gb | 120 Gb | 100-500 Mb | 1.9∼84 Kb |
| Accuracy | 99% | 99.9% | 99.9% | 99.94% | 90% | 99.999% |
| Time per run | 2 hours | 24 hours | 3–10 days | 7–14 days | 2 hours | 20 minutes - 3 hours |