Athletics at the 2008 Summer Olympics – Women's 400 metres hurdles Round 1 Round 1

| Rank_ | Heat | Name_ | Nationality | Result_ |
|---|---|---|---|---|
| 1 | 3 | Melaine Walker | Jamaica | 54.46 |
| 2 | 4 | Ekaterina Bikert | Russia | 55.15 |
| 3 | 3 | Anastasiya Rabchenyuk | Ukraine | 55.18 |
| 4 | 2 | Tasha Danvers | Great Britain | 55.19 |
| 5 | 3 | Tsvetelina Kirilova | Bulgaria | 55.22 |
| 6 | 2 | Anastasiya Ott | Russia | 55.34 |
| 7 | 4 | Anna Jesień | Poland | 55.35 |
| 8 | 1 | Tiffany Ross-Williams | United States | 55.51 |
| 9 | 1 | Irina Obedina | Russia | 55.71 |
| 10 | 2 | Nickiesha Wilson | Jamaica | 55.75 |
| 11 | 1 | Satomi Kubokura | Japan | 55.82 |
| 12 | 4 | Zuzana Hejnová | Czech Republic | 55.91 |
| 13 | 3 | Queen Harrison | United States | 55.96 |
| 14 | 2 | Angela Morosanu | Romania | 56.07 |
| 15 | 2 | Sheena Tosta | United States | 56.12 |