List of cities in the Americas by year of foundation List of cities in the Americas by year of foundation List of cities in the Americas by year of foundation

| Year_ | City | State_province_dept_etc_ | Country | Notes_ |
|---|---|---|---|---|
| 1558 | Mérida | Mérida | Venezuela |  |
| 1560 | Ipojuca | Pernambuco | Brazil |  |
| 1561 | Santa Cruz de la Sierra | Santa Cruz | Bolivia |  |
| 1561 | San Cristobal | Táchira | Venezuela |  |
| 1562 | San Juan | San Juan | Argentina |  |
| 1562 | Río Grande | Zacatecas | Mexico |  |
| 1562 | Susticacán | Zacatecas | Mexico |  |
| 1563 | Cartago | Cartago | Costa Rica | Oldest continuously inhabited European established settlement in Costa Rica. |
| 1564 | São Mateus | Espírito Santo | Brazil |  |
| 1565 | Rio de Janeiro | Rio de Janeiro | Brazil |  |
| 1565 | Saint Augustine | Florida | United States | Oldest continuously inhabited European established settlement in the continental United States. Preceded by Puerto Rican colonies, Pensacola, Florida, which was destroyed in 1559, and Fort Caroline, destroyed in 1565. |
| 1565 | Tucumán | Tucumán | Argentina |  |