USA South Athletic Conference Former members Former members

| Institution | Location_ | Nickname | Founded | Type_ | Enrollment | Joined | Left_ | Current_Conference |
|---|---|---|---|---|---|---|---|---|
| Chowan University | Murfreesboro, North Carolina | Hawks | 1848 | Private | 1260 | 2000 | 2004 | CIAA (NCAA Division II) |
| Christopher Newport University | Newport News, Virginia | Captains | 1960 | Public | 4793 | 1972 | 2013 | Capital |
| College of Charleston | Charleston, South Carolina | Cougars | 1770 | Private | 11320 | 1963 | 1970 | CAA (NCAA Division I) |
| Lynchburg College | Lynchburg, Virginia | Fighting Hornets | 1903 | Private | 2500 | 1963 | 1976 | ODAC |
| Shenandoah University | Winchester, Virginia | Hornets | 1875 | Private | 1767 | 1992 | 2012 | ODAC |
| St. Andrews Presbyterian University | Laurinburg, North Carolina | Knights | 1958 | Private | 600 | 1963 | 1988 | AAC (NAIA) |