2009–10 UEFA Europa League Play-off round Play-off round

| Team_1 | Agg_ | Team_2 | _1st_leg | _2nd_leg |
|---|---|---|---|---|
| CSKA Sofia | 2–1 † | Dynamo Moscow | 0–0 | 2–1 |
| Racing Genk | 3–6 | Lille | 1–2 | 2–4 |
| Bnei Yehuda | 0–2 † | PSV Eindhoven | 0–1 | 0–1 |
| Lazio | 3–1 | Elfsborg | 3–0 | 0–1 |
| Trabzonspor | 2–3 | Toulouse | 1–3 | 1–0 |
| Partizan | 3–1 | MŠK Žilina | 1–1 | 2–0 |
| Baku | 2–8 | Basel | 1–3 | 1–5 |
| Ajax | 7–1 | Slovan Bratislava | 5–0 | 2–1 |
| Sivasspor | 0–5 † | Shakhtar Donetsk | 0–3 | 0–2 |
| Brøndby | 3–4 | Hertha BSC | 2–1 | 1–3 |
| Athletic Bilbao | 4–3 | Tromsø | 3–2 | 1–1 |
| Sarajevo | 2–3 | CFR Cluj | 1–1 | 1–2 |
| Rapid Wien | 2–2 ( a ) | Aston Villa | 1–0 | 1–2 |
| Steaua Bucureşti | 5–1 | St Patrick`s Athletic | 3–0 | 2–1 |
| Maribor | 0–3 † | Sparta Prague | 0–2 | 0–1 |
| Nacional | 5–4 | Zenit St. Petersburg | 4–3 | 1–1 |