2010 Copa Colombia Round of 16 Round of 16

| Team_1 | Points | Team_2 | _1st_leg | _2nd_leg |
|---|---|---|---|---|
| Deportivo Pasto | 0–6 | Deportivo Pereira | 1–2 | 0–3 |
| Boyacá Chicó | 1–4 | Atlético Nacional | 2–2 | 1–2 |
| Atlético La Sabana | 3–3 ( gd ) | Cúcuta Deportivo | 1–0 | 0–4 |
| Atlético Huila | 3–3 ( gd ) | La Equidad | 2–1 | 0–2 |
| Junior | 3–3 (2–4 pk ) | Deportivo Cali | 1–0 | 2–3 |
| Itagüí Ditaires | 4–1 | Deportes Tolima | 1–0 | 2–2 |
| Atlético Bucaramanga | 3–3 (1–2 pk ) | Millonarios | 1–2 | 2–1 |