Athletics at the 2008 Summer Olympics – Men's 400 metres Semifinal 2 Semifinal 2

| Rank_ | Lane | Athlete | Nationality | Time_ | React |
|---|---|---|---|---|---|
| 1 | 6 | Leslie Djhone | France | 44.79 | 0.159 |
| 2 | 4 | David Neville | United States | 44.91 | 0.190 |
| 3 | 5 | Joel Milburn | Australia | 45.06 | 0.187 |
| 4 | 9 | Ricardo Chambers | Jamaica | 45.09 | 0.220 |
| 5 | 3 | Jonathan Borlée | Belgium | 45.11 | 0.191 |
| 6 | 8 | James Godday | Nigeria | 45.24 | 0.185 |
| 7 | 2 | Andretti Bain | Bahamas | 45.52 | 0.196 |
| 8 | 7 | Andrew Steele | Great Britain | 45.59 | 0.216 |