Athletics at the 2008 Summer Olympics – Men's 400 metres Heat 1 Heat 1

| Rank_ | Lane | Athlete | Nationality | Time_ | React |
|---|---|---|---|---|---|
| 1 | 4 | Leslie Djhone | France | 45.12 | 0.190 |
| 2 | 5 | David Neville | United States | 45.22 | 0.189 |
| 3 | 6 | William Collazo | Cuba | 45.37 | 0.180 |
| 4 | 8 | Kévin Borlée | Belgium | 45.43 | 0.149 |
| 5 | 9 | Denis Alekseyev | Russia | 45.52 | 0.299 |
| 6 | 3 | Young Talkmore Nyongani | Zimbabwe | 45.89 | 0.249 |
| 7 | 7 | Eric Milazar | Mauritius | 46.06 | 0.209 |
| 8 | 2 | Gakologelwang Masheto | Botswana | 46.29 | 0.183 |