2007–08 Isthmian League Second Round Second Round

| Tie_no | Home_team | Score | Away_team | Attendance |
|---|---|---|---|---|
| 3 | AFC Wimbledon | 0–2 | Whyteleafe | 529 |
| 4 | AFC Hornchurch | 3–0 | Tilbury | 182 |
| 5 | Arlesey Town | 4–0 | Enfield Town | 75 |
| 6 | Ashford Town (Middx) | 2–1 | Walton & Hersham | 93 |
| 7 | Ashford Town (Kent) | 1–3 | Burgess Hill Town | 110 |
| 8 | Billericay Town | 0–3 | AFC Sudbury | 202 |
| 9 | Bury Town | 2–3 | Chelmsford City | 206 |
| 10 | Carshalton Athletic | 4–0 | Corinthian-Casuals | 75 |
| 11 | Dartford | 3–0 | Aveley | 318 |
| 12 | Dover Athletic | 2–3 | Eastbourne Town | 151 |
| 13 | Dulwich Hamlet | 0–0 | Tooting & Mitcham United | 149 |