Swimming at the 2000 Summer Olympics – Women's 100 metre butterfly Semifinal 1 Semifinal 1

| Rank_ | Lane | Name_ | Nationality | Time_ |
|---|---|---|---|---|
| 1 | 4 | Jenny Thompson | United States | 58.18 |
| 2 | 3 | Martina Moravcová | Slovakia | 58.49 |
| 3 | 7 | Diana Mocanu | Romania | 59.12 |
| 4 | 5 | Otylia Jędrzejczak | Poland | 59.14 |
| 5 | 2 | Natalya Sutyagina | Russia | 59.30 |
| 6 | 6 | Mette Jacobsen | Denmark | 59.75 |
| 7 | 8 | Cécile Jeanson | France | 59.80 |
| 8 | 1 | Anna-Karin Kammerling | Sweden | 1:00.40 |