Mid-States Football Association Former members Former members

| Institution | Location_ | Founded | Type_ | Enrollment | Joined | Left_ | Nickname | Primary_Conference_when_joining_the_MSFA | Current_Primary_Conference |
|---|---|---|---|---|---|---|---|---|---|
| McKendree University | Lebanon, Illinois | 1828 | Private | 3220 | 1998-99 | 2010-11 | Bearcats | American Midwest | GLVC (NCAA Division II) |
| Ohio Dominican University | Columbus, Ohio | 1911 | Private | 3052 | 2004-05 | 2008-09 | Panthers | American Mideast | GLIAC (NCAA Division II) |
| Quincy University | Quincy, Illinois | 1860 | Private | 1169 | 2003-04 | 2011-12 | Hawks | GLVC (NCAA Division II) | GLVC (NCAA Division II) |
| Tiffin University | Tiffin, Ohio | 1888 | Private | 6816 | 1994-95 | 2001-02 | Dragons | American Mideast | GLIAC (NCAA Division II) |
| Trine University | Angola, Indiana | 1884 | Private | 1779 | 1996-97 | 2002-03 | Thunder | WHAC | MIAA (NCAA Division III) |
| Urbana University | Urbana, Ohio | 1850 | Private | 1505 | 1994-95 | 2007-08 | Blue Knights | American Mideast | G-MAC (NCAA Division II) |
| Walsh University | North Canton, Ohio | 1960 | Private | 2500 | 1996-97 | 2010-11 | Cavaliers | American Mideast | GLIAC (NCAA Division II) |