Swimming at the 2007 World Aquatics Championships – Women's 100 metre freestyle Preliminaries Preliminaries

| Rank_ | Heat_Lane | Name_ | Nationality | Time_ |
|---|---|---|---|---|
| 1 | 16 (5) | Natalie Coughlin | USA | 00: 54.04 |
| 2 | 16 (7) | Erica Morningstar | Canada | 00: 54.30 |
| 3 | 16 (4) | Lisbeth Lenton | Australia | 00: 54.32 |
| 4 | 17 (4) | Britta Steffen | Germany | 00: 54.60 |
| 5 | 15 (2) | Josefin Lillhage | Sweden | 00: 54.74 |
| 6 | 17 (5) | Jodie Henry | Australia | 00: 54.76 |
| 7 | 17 (6) | Fran Halsall | Great Britain | 00: 54.80 |
| 8 | 15 (5) | Marleen Veldhuis | Netherlands | 00: 54.89 |
| 9 | 15 (4) | Amanda Weir | USA | 00: 55.03 |
| 10 | 16 (3) | Alena Popchanka | France | 00: 55.11 |
| 11 | 15 (6) | Yanwei Xu | China | 00: 55.24 |
| 12 | 17 (2) | Hanna-Maria Seppälä | Finland | 00: 55.33 |