TranSouth Athletic Conference Former members Former members

| Institution | Location_ | Founded | Type_ | Enrollment | Nickname | Joined | Left_ | Current_Conference |
|---|---|---|---|---|---|---|---|---|
| Williams Baptist College | Walnut Ridge, Arkansas | 1941 | Private | 700 | Eagles | 1996 | 2001 | American Midwest |
| Lyon College | Batesville, Arkansas | 1872 | Private | 600 | Scots | 1997 | 2012 | American Midwest |
| Lambuth University | Jackson, Tennessee | 1843 | Private | 800 | Eagles | 2006 | 2009 | closed in 2011 |