2007–08 Isthmian League Second Round Second Round

| Tie_no | Home_team | Score | Away_team | Attendance |
|---|---|---|---|---|
| Tooting & Mitcham United advance 5–3 on penalties | Tooting & Mitcham United advance 5–3 on penalties | Tooting & Mitcham United advance 5–3 on penalties | Tooting & Mitcham United advance 5–3 on penalties | Tooting & Mitcham United advance 5–3 on penalties |
| 14 | Edgware Town | 3–1 | Staines Town | 48 |
| 15 | Great Wakering Rovers | 4–1 | Ilford | 115 |
| 16 | Harlow Town | 1–3 | Ware | 131 |
| 17 | Harrow Borough | 0–5 | Metropolitan Police | 76 |
| 18 | Hendon | 2–3 | Boreham Wood | 101 |
| 19 | Horsham | 4–0 | Hastings United | 162 |
| 20 | Horsham YMCA | 4–3 | Folkestone Invicta | 50 |
| 21 | Kingstonian | 3–1 | Cray Wanderers | 179 |
| 22 | Leatherhead | 3–2 | Croydon Athletic | 67 |
| 23 | Maidstone United | 5–2 | Canvey Island | 131 |
| 24 | Margate | 2–3 | East Thurrock United | 174 |
| 25 | Molesey | 2–3 | Wealdstone | 106 |
| 26 | Potters Bar Town | 3–1 | Leyton | 41 |
| 27 | Ramsgate | 4–2 | Chatham Town | 87 |
| 28 | Redbridge | 2–1 | Sittingbourne | 41 |