List of cities in the Americas by year of foundation List of cities in the Americas by year of foundation List of cities in the Americas by year of foundation

| Year_ | City | State_province_dept_etc_ | Country | Notes_ |
|---|---|---|---|---|
| 1538 | Sucre | Chuquisaca | Bolivia | Originally called Ciudad de la Plata de la Nueva Toledo and the oldest continuously inhabited European established settlement in Bolivia. |
| 1540 | Arequipa | Arequipa | Peru |  |
| 1540 | Ayacucho | Ayacucho | Peru |  |
| 1540 | Campeche | Campeche | Mexico |  |
| 1540 | Yuriria | Guanajuato | Mexico |  |
| 1540 | Campeche | Campeche | Mexico | Founded on the site of Mayan village Kimpech. |
| 1541 | Huánuco | Huánuco | Peru |  |
| 1541 | Moquegua | Moquegua | Peru |  |
| 1541 | Santiago | Santiago | Chile | Oldest continuously inhabited European established settlement in Chile. |
| 1542 | Guadalajara | Jalisco | Mexico |  |
| 1542 | Genaro Codina | Zacatecas | Mexico |  |
| 1542 | San Miguel de Allende | Guanajuato | Mexico |  |
| 1542 | Mérida | Yucatan | Mexico | Built on the site of Mayan city T`ho. |
| 1542 | San Luis de la Paz | Guanajuato | Mexico |  |
| 1543 | Antigua Guatemala | Sacatepéquez | Guatemala |  |
| 1543 | Cobán | Alta Verapaz | Guatemala |  |