Mid-South Conference Full members Full members

| Institution | Location_Population | Founded | Type_ | Enrollment | Nickname_Colors | Football |
|---|---|---|---|---|---|---|
| University of Pikeville | Pikeville, Kentucky (6,903) | 1889 | Private / Presbyterian | 1156 | Bears (Orange) | Yes |
| University of Rio Grande | Rio Grande, Ohio (915) | 1876 | Private / No affiliation | 1893 | RedStorm (Red & Black) | No |
| Shawnee State University | Portsmouth, Ohio (20,909) | 1986 | Public | 4300 | Bears (Royal Blue & Gray) | No |
| St. Catharine College | St. Catharine, Kentucky (1,000) | 1873 | Private / Catholic | 700 | Patriots (Purple & Gold) | No |