1980–81 FA Cup First round proper First round proper

| Tie_no | Home_team | Score | Away_team | Date_ |
|---|---|---|---|---|
| 31 | Kettering Town | 1–1 | Maidstone United | 22 November 1980 |
| Replay | Maidstone United | 0–0 | Kettering Town | 26 November 1980 |
| Replay | Maidstone United | 3–1 | Kettering Town | 1 December 1980 |
| 32 | Wigan Athletic | 2–2 | Chesterfield | 22 November 1980 |
| Replay | Chesterfield | 2–0 | Wigan Athletic | 25 November 1980 |
| 33 | Boston United | 0–4 | Rotherham United | 22 November 1980 |
| 34 | Harlow Town | 0–2 | Charlton Athletic | 22 November 1980 |
| 35 | Colchester United | 3–0 | Portsmouth | 22 November 1980 |
| 36 | Gravesend & Northfleet | 1–2 | St Albans City | 22 November 1980 |
| 37 | Burscough | 1–2 | Altrincham | 22 November 1980 |
| 38 | Mossley F.C. | 1–0 | Crewe Alexandra | 22 November 1980 |
| 39 | Sutton Coldfield Town | 0–2 | Doncaster Rovers | 22 November 1980 |
| 40 | Oxford United | 1–0 | Aldershot | 22 November 1980 |