List of cities in the Americas by year of foundation List of cities in the Americas by year of foundation List of cities in the Americas by year of foundation

| Year_ | City | State_province_dept_etc_ | Country | Notes_ |
|---|---|---|---|---|
| 1543 | Santa Cruz Verapaz | Alta Verapaz | Guatemala |  |
| 1545 | Potosí | Potosí | Bolivia |  |
| 1546 | Neyba | Bahoruco | Dominican Republic |  |
| 1546 | Tepezalá | Aguascalientes | Mexico |  |
| 1548 | Asientos | Aguascalientes | Mexico |  |
| 1548 | La Paz | La Paz | Bolivia |  |
| 1548 | Pánuco | Zacatecas | Mexico |  |
| 1548 | Zacatecas | Zacatecas | Mexico |  |
| 1549 | Salvador | Bahia | Brazil |  |
| 1550 | Concepción | Concepción | Chile |  |
| 1551 | Vitória | Espírito Santo | Brazil |  |
| 1552 | Sonsonate | Sonsonate | El Salvador |  |
| 1553 | Santiago del Estero | Santiago del Estero | Argentina | Oldest continuously inhabited European established settlement in Argentina. |
| 1553 | São Bernardo do Campo | São Paulo | Brazil |  |
| 1554 | Embu | São Paulo | Brazil |  |
| 1554 | São Paulo | São Paulo | Brazil |  |
| 1555 | Saín Alto | Zacatecas | Mexico |  |
| 1556 | Chalchihuites | Zacatecas | Mexico |  |
| 1557 | Santo Amaro | Bahia | Brazil |  |