2009–10 Mid-American Conference season Affiliate membership Affiliate membership

| Institution | Nickname | Location_ | Founded | Affiliation | Enrollment | Sport |
|---|---|---|---|---|---|---|
| Chicago State University | Cougars | Chicago, Illinois | 1867 | Public | 7131 | Men`s tennis |
| University of Evansville | Purple Aces | Evansville, Indiana | 1854 | Private | 3050 | Men`s swimming |
| Florida Atlantic University | Owls | Boca Raton, Florida | 1961 | Public | 26245 | Men`s soccer |
| Hartwick College | Hawks | Oneonta, New York | 1797 | Private | 1520 | Men`s soccer |
| Missouri State University | Lady Bears, Bears | Springfield, Missouri | 1905 | Public | 21425 | Field hockey, men`s swimming |
| Southern Illinois University Carbondale | Salukis | Carbondale, Illinois | 1869 | Public | 21000 | Men`s swimming |