Mid-South Conference Full members Full members

| Institution | Location_Population | Founded | Type_ | Enrollment | Nickname_Colors | Football |
|---|---|---|---|---|---|---|
| Bluefield College | Bluefield, Virginia (5,444) | 1922 | Private / Baptist General Association of Virginia | 793 | Rams (Blue & Red) | Yes |
| Campbellsville University | Campbellsville, Kentucky (9,108) | 1906 | Private / SBC | 3318 | Tigers (Maroon) | Yes |
| Cumberland University | Lebanon, Tennessee (20,235) | 1842 | Private / No Affiliation | 1345 | Bulldogs (Maroon & White) | Yes |
| University of the Cumberlands | Williamsburg, Kentucky (5,245) | 1887 | Private / SBC | 1743 | Patriots (Red, Navy, Light Gray) | Yes |
| Georgetown College | Georgetown, Kentucky (29,098) | 1829 | Private / SBC | 1400 | Tigers (Orange & Black) | Yes |
| Lindsey Wilson College | Columbia, Kentucky (4,452) | 1903 | Private / Methodist | 2677 | Blue Raiders (Blue & White) | Yes |