List of cities in the Americas by year of foundation List of cities in the Americas by year of foundation List of cities in the Americas by year of foundation

| Year_ | City | State_province_dept_etc_ | Country | Notes_ |
|---|---|---|---|---|
| 1532 | São Vicente | São Paulo | Brazil | Oldest continuously inhabited European established settlement in Brazil, oldest Portuguese established settlement in the New World. |
| 1532 | Tepic | Nayarit | Mexico | As capital of Nueva Galicia [7] |
| 1533 | Cananéia | São Paulo | Brazil | Second Oldest City in Brazil |
| 1533 | Cartagena de Indias | Bolívar | Colombia |  |
| 1534 | Otavalo | Imbabura | Ecuador |  |
| 1534 | Quito | Pichincha | Ecuador | Oldest continuously inhabited European established settlement in Ecuador. |
| 1534 | Spanish Town | Saint Catherine | Jamaica | Founded by the Spanish as Santiago de la Vega or St. Jago de la Vega. Oldest continuously inhabited European established settlement in Jamaica. |
| 1534 | Trujillo | La Libertad | Peru | Oldest continuously inhabited European established settlement in Peru. |
| 1535 | Lima | Lima | Peru |  |