2008 IAAF World Indoor Championships – Women's 400 metres Semifinals Semifinals

| Heat | Lane | Name_ | Country | Mark |
|---|---|---|---|---|
| 1 | 5 | Olesya Zykina | Russia | 51.75 |
| 1 | 3 | Antonina Yefremova | Ukraine | 51.79 PB |
| 1 | 6 | Moushaumi Robinson | United States | 51.85 PB |
| 1 | 1 | Racheal Nachula | Zambia | 53.30 NR |
| 1 | 2 | Makelesi Batimala | Fiji | 54.23 |
| 1 | 4 | Christy Ekpukhon Ihunaeqbo | Nigeria | DNS |
| 2 | 6 | Natalya Nazarova | Russia | 51.62 |
| 2 | 3 | Shareese Woods | United States | 51.87 PB |
| 2 | 4 | Angela Morosanu | Romania | 52.83 SB |
| 2 | 2 | Zuzana Hejnová | Czech Republic | 53.16 |
| 2 | 5 | Amantle Montsho | Botswana | 53.21 |
| 2 | 1 | Nataliya Pyhyda | Ukraine | 53.33 |