The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue
The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue
Yazar: Kahraman, Cemil; Alver, Ahmet; Şentürk, Ayşe; Akça, İmran İnce
Dergi: Dicle Tıp Dergisi, Vol. 45 No. 4, 2018
Anahtar kelimeler: Health Care Administration ; Sağlık Kurumları Yönetimi ; Carbonic anhydrase III,brown adipose tissue,obesity,N-acetylcysteine

Objective:High-fat foods increase adipose tissue size, and induce obesity. Althoughcarbonic anhydrase III is abundantly found in brown adipose tissue, itsfunction is not fully defined. In this study, we investigated the relationshipbetween carbonic anhydrase III enzyme mRNA expression and malondialdehyde,oxidative stress marker, in brown adipose tissue of rats that were fed high-fatdiets. In addition, we investigated potential effect of N-acetylcysteine as anantioxidant in this relationship. Methods: Inour study three experimental groups were formed and each contained 6 rats(control, obese, and antioxidant groups). The experimental groups were fed fora duration of 85 days with high fat diets. In these groups, carbonic anhydraseIII mRNA expression, total carbonic anhydrase hydratase activitie, andmalondialdehyde levels were measured in brown adipose tissues dissected fromrat scapula regions.Results:According to our findings, carbonic anhydrase III mRNA expression was higher inthe obese group than in the control group (p = 0.004), and malondialdehydelevels were lower in the obese group than in the control group (p = 0.03). Itwas observed that carbonic anhydrase III mRNA expression was higher in theantioxidant group than in the control group (p = 0.006), and malondialdehydelevels were lower in the antioxidant group than in the control group (p =0.006). In addition, in the obese group carbonic anhydrase III mRNA expressionwas higher than in the antioxidant group (p=0.01). Conclusion:In brown adipose tissue of rats that were fed high-fat diets, this study showedthat the carbonic anhydrase III mRNA expression increased and themalondialdehyde level decreased.

Objective:High-fat foods increase adipose tissue size, and induce obesity. Althoughcarbonic anhydrase III is abundantly found in brown adipose tissue, itsfunction is not fully defined. In this study, we investigated the relationshipbetween carbonic anhydrase III enzyme mRNA expression and malondialdehyde,oxidative stress marker, in brown adipose tissue of rats that were fed high-fatdiets. In addition, we investigated potential effect of N-acetylcysteine as anantioxidant in this relationship. Methods: Inour study three experimental groups were formed and each contained 6 rats(control, obese, and antioxidant groups). The experimental groups were fed fora duration of 85 days with high fat diets. In these groups, carbonic anhydraseIII mRNA expression, total carbonic anhydrase hydratase activitie, andmalondialdehyde levels were measured in brown adipose tissues dissected fromrat scapula regions.Results:According to our findings, carbonic anhydrase III mRNA expression was higher inthe obese group than in the control group (p = 0.004), and malondialdehydelevels were lower in the obese group than in the control group (p = 0.03). Itwas observed that carbonic anhydrase III mRNA expression was higher in theantioxidant group than in the control group (p = 0.006), and malondialdehydelevels were lower in the antioxidant group than in the control group (p =0.006). In addition, in the obese group carbonic anhydrase III mRNA expressionwas higher than in the antioxidant group (p=0.01). Conclusion:In brown adipose tissue of rats that were fed high-fat diets, this study showedthat the carbonic anhydrase III mRNA expression increased and themalondialdehyde level decreased.