Embriyosu Derin Dondurucuda Öldürülmü ş Ephestia küehniella  Zeller  Lepidoptera: Pyralidae  Yumurtalar ı nda Trichogramma turkeiensis  Kostadinov ve Trichogramma embryophagum Hartig   Hymenoptera:Trichogrammatidae 'un Yetiştirilmesi  Üzerinde Ara ştı rmalar
Investigations on the Mass Culture of Trichogramma turkeiensis Kostadinov and Trichogramma embryophagum Hartig   Hymenoptera: Trichogrammatidae  on Killed embryos of Ephestia kuehniella Zeller  Lepidoptera: Pyralidae   Eggs Using Deep Freeze Method
Yazar: Ay, Recep; Gürkan, M.oktay
Dergi: Journal of Agricultural Sciences, Vol. 03 No. 01, 0
Anahtar kelimeler: Trichogramma turkeiensis, Tnchogramma embryophagum, mass rearing technicque. ; Trichogramma turkeiensis, Trichogramma embryophagum, kitle üretimi.

Değirmen güvesi yumurtalar ı derin dondurucuda farkl ı s ı cakl ı k  -20 °C ve -25 °C , farkl ı sürelerde   60 ve 120  dakika  tutularak öldürülmü ştür. T. embryophagum kontrol olarak kullan ı lan ve -25 ° C de 60, 120 dakika tutulan Değirmen  güvesi yumurtalar ı n ı sı ras ı yla %80.4, 23.2, 17.2 oran ı nda, T. turkeiensis ise ayn ı s ı raya göre %86.8, 24.4 ve 12.0 oran ı nda  parazitlemiştir. T. emryophagum kontrol olarak kullan ı lan ve -20 °C de 60, 120 dakika tutulan Değ irmen güvesi yumurtalar ı n ı  s ı ras ı yla %78.4, 64.0 ve 56.4 oran ı nda, T. turkeiensis %87.2, 67.2, 58.0 oran ı nda parazitledi ğ i tespit edilmi ştir. Embriyosu  derin dondurucuda de ğ i şik sürelerde   60 ve 120 dk.  tutularak öldürülmü ş ve kontrol olarak kullan ı lan konukçu yumurtalar ı 5  günde siyahla şm ışt ı r ve parazitoidler 14 günde geli şmiştir. Embriyosu derin dondurucuda öldürülen bütün konukçu  yumurtalar ı nda geli şen parazitiodlerin di şi oldugu belirlenmi ştir. Kontrol olarak kullan ı lan ve derin dondurucuda -20 °C de 60,  120 dakika tutulan Değ irmen güvesi yumurtas ı nda gelişen T. embryophagum dişisinin yaşam uzunluğu s ı ras ı yla 16.80, 19.08  ve 19.20 gün, T. turkeiensis'in 18.60, 19.60 ve 20.52 gün oldu ğ u saptanm ışt ı r. Derin dondurucuda -20 °C s ı cakl ı ta O, 60,  120 dakika tutulan Değ irmen güvesi yumurtasinda geli şen bir T. emryophagum di şisi yaşam ı boyunca s ı ras ı yla ortalama  100.00, 100.84 ve 105.56, T. turkeiensis ise 99.12, 98.88 ve 104.12 normal De ğ irmen güvesi yumurtas ı parazitledi ği  saptanm ışt ı r. Normal ve embriyosu derin dondurucuda öldürülen konukçu yumurtas ı nda gelişen parazitoid döllerinin gelişme  süreleri ve cinsiyet oranlar ı aras ı nda önemli bir fark tesbit edilmemi ştir.

Ephestia kuehniella eggs were killed by keeping in the deep freeze  at -20 °C and -25 °C for 60 and 120  minutes respectivelly . T. embryophagum parasited the E. kuehniella eggs which were normal and which had been kept at -  25 °C for 60 and 120 minutes at the rates of 80.4, 23.2, and 17.2 %, respectively. T. turkeiensis on the other hand,  parasitized the eggs at the rates of 86.8, 24.4, and 12.0 % respectively at same conditions. T. embryophagum parasitized the  E. kuehniella eggs which were normal and which had been kept at -20 ° C for 60 and 120 minutes at the rates of 78.4, 64.0,  and 56.4 %respectively. The percentages of parasitized E. kuehnilla eggs by T. turkeiests was 87.2, 67.2, and 58.0 %  respectively at same conditions. Host eggs whose embryos were killed by keeping in the deep freeze at different periods   60  and 120 min.   and controls were also turned black in 5 days and parasitoids had grown in 14 days. All parasitoids which had  developed from host eggs, their embryos were killed by keeping in the deep freeze, had been recorded as females. The mean  longevites of parasitoid females developed from E. kuehniella eggs which were normal and which had been kept at -20 °C for  60 and 120 minutes were recorded as 16.80, 19.08, and 19.2 days for T. embryophagum and 18.60, 19.60, and 20.52 days  for T. turkeiensis, repectively. The mean of total parasitized eggs numbers by the one parasitoid female during her life  developed from the E. kuehniella eggs which were normal and which had been kept at -20 °C for 60 and 120 minutes were  100.00, 100.84, and 105.56 eggs, respectively for T. embryophagum, 99.2, 98.88, and 104.12 eggs, respectively for T.  turkeiensis. It was recorded that there was no difference for range of sex and developmental period between offspring of  parasitoids which had developed from host eggs whose embryos were killed by deep freeze and from normal eggs.