Bazı bitki ekstraktları ve kimyasal maddelerin tütün mozaik virüsü enfeksiyonuna etkilerinin araştırılması
Investigation of the effects of some plant extracts and chemical  substances on the infection of <em>Tobacco  mosaic virus</em>
Yazar: Arslan, Neziha; Erkan, Semih
Dergi: Bitki Koruma Bülteni, Vol. 52 No. 2, 2012
Anahtar kelimeler: Tütün, tütün mozaik virüsü, engelleme, bitki ekstraktı, kimyasal madde.

In the present study, the inhibitory effects of 14  plant extracts and 15 chemical substances were examined on the infection of Tobacco mosaic virus (TMV). According to  the results of experiments on test plants, plant extracts inhibited virus  infection at the ratios of 25.92 % to 97.19 % while the inhibition levels of  chemical substances on virus infection ranged between 10.24 % and 98.30 %. The  findings showed that the highest inhibition levels were obtained with three  plant extracts (Dianthus caryophyllus, Capsicum annuum and Yucca elephantipes) and three chemicals (Sodium dodecylsulfate, Triton  X-100 and HuwaSan). Therefore, the mentioned extracts and chemicals were  selected for further steps of the study at which the effects of some factors as  dilution, temperature and application times on the inhibition of virus  infection were examined. Considering the data from assays on test plants, it  was observed that inhibitory activity of D. caryophyllus and sodium  dodecylsulfate was not affected much more with dilution and temperature  fluctuations. Moreover, the results showed that extract from D. caryophyllus had more inhibitory effect when it was applied in 2 and 4 hours before  virus inoculation and on the contrary Sodium dodecylsulfate inhibited virus  infection at higher level when used in 2 and 4 hours after virus application.  Later, in pot experiments performed on tobacco (Nicotiana tabacum L. cv.  Akhisar 97) plants, it was observed that the application of D. caryophyllus extract  and reduction of virus concentration in the tobacco plants by spreying sodium  dodecylsulfate before or after virus inoculation was determined by results of  DAS-ELISA test.

In the present study, the inhibitory effects of 14  plant extracts and 15 chemical substances were examined on the infection of Tobacco mosaic virus (TMV). According to  the results of experiments on test plants, plant extracts inhibited virus  infection at the ratios of 25.92 % to 97.19 % while the inhibition levels of  chemical substances on virus infection ranged between 10.24 % and 98.30 %. The  findings showed that the highest inhibition levels were obtained with three  plant extracts (Dianthus caryophyllus, Capsicum annuum and Yucca elephantipes) and three chemicals (Sodium dodecylsulfate, Triton  X-100 and HuwaSan). Therefore, the mentioned extracts and chemicals were  selected for further steps of the study at which the effects of some factors as  dilution, temperature and application times on the inhibition of virus  infection were examined. Considering the data from assays on test plants, it  was observed that inhibitory activity of D. caryophyllus and sodium  dodecylsulfate was not affected much more with dilution and temperature  fluctuations. Moreover, the results showed that extract from D. caryophyllus had more inhibitory effect when it was applied in 2 and 4 hours before  virus inoculation and on the contrary Sodium dodecylsulfate inhibited virus  infection at higher level when used in 2 and 4 hours after virus application.  Later, in pot experiments performed on tobacco (Nicotiana tabacum L. cv.  Akhisar 97) plants, it was observed that the application of D. caryophyllus extract  and reduction of virus concentration in the tobacco plants by spreying sodium  dodecylsulfate before or after virus inoculation was determined by results of  DAS-ELISA test.