Meloidogyne incognita (Kofoid&White) Chitwood (Nematoda: Heteroderidae)'nın   antagonist kök bakterileri ile mücadelesine yönelik ön çalışmalar
Preliminary investigations on suppression of Meloidogyne incognita (Kofoid &   White) Chitwood (Nematoda: Heteroderidae) by antagonistic rhizobacteria
Yazar: Uslu, Galip Kaşkavalci Hatice Özaktan Ahmet Ha
Dergi: Türkiye Entomoloji Dergisi, Vol. 30 No. 3, 2014
Anahtar kelimeler: Kök-ur nematodları, hıyar, Meloidogyne spp., antagonist kök bakterileri, Pseudomonas fluorescens

Fluorescent Pseudomonad strains have been obtained from diseased second juveniles from Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949(Nematoda: Heteroderidae) infected tomato roots. After the screening in vivo made by 5 different isolated strains, Strain Pat1 has been selected to test the antagonistic effects on the root-knot nematodes. Strain Pat1 has been identified as non plant pathogenic Pseudomonas fluorescens Migula, 1895 (Proteobacteria: Pseudomonadaceae) according to the test result. Cucumber (Cucumis sativus) cv Sardes has been used as test plant. Bacterial suspension (109 cfu/ml) was applied by three different ways: 1) Seed bacterization; 2) Seed bacterization plus seedling drenching; and 3) Seedling drenching. The eggs of rootknot nematodes have been obtained from diseased tomato roots by blender-sieve method for infection cucumber seedlings with root-knot nematodes. 20 000 eggs/plant have been given to cucumber seedlings during transplanting. The plants have been observed for symptom development for 10-11 weeks in climatized room under controlled conditions. Symptom development has been evaluated by using 0-4 scale for upper parts of plants, and 0-10 Zeck’s root-knot scale for the roots. When seed bacterization and seed bacterization plus seedling drenching applications compared with positive control; plants infected with root-knot nematodes alone, it was determined that disease symptoms on upper parts of the plants were reduced 35 % and 21 %; gal formation on the roots were reduced 44 % and 39 %, respectively. The experiment has been repeated twice to observe the colonization and population dynamic of antagonistic bacteria on the roots

Fluorescent Pseudomonad strains have been obtained from diseased second juveniles from Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949(Nematoda: Heteroderidae) infected tomato roots. After the screening in vivo made by 5 different isolated strains, Strain Pat1 has been selected to test the antagonistic effects on the root-knot nematodes. Strain Pat1 has been identified as non plant pathogenic Pseudomonas fluorescens Migula, 1895 (Proteobacteria: Pseudomonadaceae) according to the test result. Cucumber (Cucumis sativus) cv Sardes has been used as test plant. Bacterial suspension (109 cfu/ml) was applied by three different ways: 1) Seed bacterization; 2) Seed bacterization plus seedling drenching; and 3) Seedling drenching. The eggs of rootknot nematodes have been obtained from diseased tomato roots by blender-sieve method for infection cucumber seedlings with root-knot nematodes. 20 000 eggs/plant have been given to cucumber seedlings during transplanting. The plants have been observed for symptom development for 10-11 weeks in climatized room under controlled conditions. Symptom development has been evaluated by using 0-4 scale for upper parts of plants, and 0-10 Zeck’s root-knot scale for the roots. When seed bacterization and seed bacterization plus seedling drenching applications compared with positive control; plants infected with root-knot nematodes alone, it was determined that disease symptoms on upper parts of the plants were reduced 35 % and 21 %; gal formation on the roots were reduced 44 % and 39 %, respectively. The experiment has been repeated twice to observe the colonization and population dynamic of antagonistic bacteria on the roots