CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS
CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS
Yazar: Keskin, Şeyma; Şengül, Fatih
Dergi: Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, Vol. 32 No. 1, 2021
Anahtar kelimeler: Dentistry ; Diş Hekimliği ; Glass ionomer cements,dental pulp stem cell,mesenchymal stem cell,cytotoxicity,xCELLigence

Aim: The aim of this study was to observe the cytotoxic effect of Resin modified Glass Ionomer Cement (RMGIC) on human dental pulp stem cells (DPSCs) proliferation by using xCELLigence®, a device that measures real-time cell viability and evaluates cytotoxic effects, and to determine the CC50 value on these cells for 72 hours.Materials and Methods: DPSCs obtained from the American Type Culture Collection were seeded on E-Plates. After 24 hours, three different dilutions (100%, 10% and 1%) of the elution obtained from RMGIC were added to three wells. DMEM solution was used as the control group. Real time cell index data were acquired by using xCELLigence® device for 72 hours. In order to compare cell index values, repeated-measures analysis of variance and linear regression analysis were used.Results: In contrast to the 100% dilution of RMGIC which exhibited toxic effect on DPSCs, its 1% dilution showed proliferative effect. And 10% dilution was similar to the control group. While the coefficient of determination was above 80% in all groups, it was found to be lower only in the RMGIC 100%-dilution group by 0.7%. Also, the regression coefficient was found to be significantly different from zero in all equations except RMGIC 100%-dilution group (p<0.001). CC50 values of RMGIC at the 24th, 48th and 72nd hours were 5.07%, 5.07% and 5.08%, respectively.Conclusion: In order to provide more reliable results, CC50 values determined in our study will guide the further studies to improve RMGICs by adding different molecules to its structure for reducing its cytotoxicity.Keywords: Glass ionomer cements, dental pulp stem cell, mesenchymal stem cell, cytotoxicity, xCELLigenceRezin ile Modifiye Edilmiş Cam İyonomer Simanların Diş Pulpası Kök Hücreleri Üzerindeki Sitotoksisitesi

Aim: The aim of this study was to observe the cytotoxic effect of Resin modified Glass Ionomer Cement (RMGIC) on human dental pulp stem cells (DPSCs) proliferation by using xCELLigence®, a device that measures real-time cell viability and evaluates cytotoxic effects, and to determine the CC50 value on these cells for 72 hours.Materials and Methods: DPSCs obtained from the American Type Culture Collection were seeded on E-Plates. After 24 hours, three different dilutions (100%, 10% and 1%) of the elution obtained from RMGIC were added to three wells. DMEM solution was used as the control group. Real time cell index data were acquired by using xCELLigence® device for 72 hours. In order to compare cell index values, repeated-measures analysis of variance and linear regression analysis were used.Results: In contrast to the 100% dilution of RMGIC which exhibited toxic effect on DPSCs, its 1% dilution showed proliferative effect. And 10% dilution was similar to the control group. While the coefficient of determination was above 80% in all groups, it was found to be lower only in the RMGIC 100%-dilution group by 0.7%. Also, the regression coefficient was found to be significantly different from zero in all equations except RMGIC 100%-dilution group (p<0.001). CC50 values of RMGIC at the 24th, 48th and 72nd hours were 5.07%, 5.07% and 5.08%, respectively.Conclusion: In order to provide more reliable results, CC50 values determined in our study will guide the further studies to improve RMGICs by adding different molecules to its structure for reducing its cytotoxicity.Keywords: Glass ionomer cements, dental pulp stem cell, mesenchymal stem cell, cytotoxicity, xCELLigenceRezin ile Modifiye Edilmiş Cam İyonomer Simanların Diş Pulpası Kök Hücreleri Üzerindeki Sitotoksisitesi