Tabletlerde Mebendazolün Spektroflorimetrik Tayini
Spectrofluorimetric Assay of Mebendazole in Tablets
Yazar: Küçükkolbaşı, Semahat; Gündüz, Beniz; Kılıç, Esma
Dergi: Gazi Eğitim Fakültesi Dergisi, Vol. 24 No. 3, 2014
Anahtar kelimeler: Spectrofluorimetry, assay, benzimidazole, mebendazole, pharmaceuticals ; Spektroflorimetri, tayin, benzimidazol, mebendazol, ilaç

Bu çalışmada, tabletlerde mebendazol (MBZ) ilaç etken maddesinin tayini için  spektroflorimetrik bir yöntem geliştirildi. Bu amaçla, mebendazol ilaç etken maddesinin  çeşitli çözücülerde floresans spektrumları alınarak en uygun çözücünün dimetil sülfoksit  ve bu çözücü ortamında uyarma (&lambda;uy) ve emisyon (&lambda;em) dalga boylarının sırasıyla 390  nm ve 473,6 nm olduğu belirlendi. Kalibrasyon grafikleri çizildi ve bunların 0-2,5 mg/L  derişim aralığında doğrusal olduğu görüldü (R2= 0,9993). Doğrudan kalibrasyon  grafiklerinden ve standart katma yöntemiyle Vermazol tabletindeki mebendazol miktarı  tayin edildi. Geliştirilen spektroflorimetrik yöntemle elde edilen sonuçlar, standart USP  24 yöntemi ile elde edilen sonuçlarla karşılaşIn this study a spectrofluorimetric method has been developed for assay of mebendazole  (MBZ) drug active compound in tablets. For this purpose; taking measured  fluorescence intensities of mebendazole drug active compound in various solvents, it  was determined that suitable solvent was dimethyl sulfoxide and the wavelengths of  excitation and emission were 390 nm and 473,6 nm, respectively. Calibration graphs  were drawn and the fluorescence intensity was linearly related to the drug  concentration which is between 0-2,5 mg/L (R2= 0,9993). Mebendazole in Vermazol  tablet was determined both from calibration graph and from standard addition method.  The validity of the method was tested by the recovery studies of standard addition to  pharmaceuticals and the result was found to be satisfactory. The results obtained from  spektrofluorometric method were compared with official USP 24 method and weSpektroflorimetri, tayin, benzimidazol, mebendazol, ilaçSpectrofluorimetry, assay, benzimidazole, mebendazole, pharmaceuticals Tam Metin

In this study a spectrofluorimetric method has been developed for assay of mebendazole  (MBZ) drug active compound in tablets. For this purpose; taking measured  fluorescence intensities of mebendazole drug active compound in various solvents, it  was determined that suitable solvent was dimethyl sulfoxide and the wavelengths of  excitation and emission were 390 nm and 473,6 nm, respectively. Calibration graphs  were drawn and the fluorescence intensity was linearly related to the drug  concentration which is between 0-2,5 mg/L (R2= 0,9993). Mebendazole in Vermazol  tablet was determined both from calibration graph and from standard addition method.  The validity of the method was tested by the recovery studies of standard addition to  pharmaceuticals and the result was found to be satisfactory. The results obtained from  spektrofluorometric method were compared with official USP 24 method and weSpektroflorimetri, tayin, benzimidazol, mebendazol, ilaçSpectrofluorimetry, assay, benzimidazole, mebendazole, pharmaceuticals Tam Metin