DİŞ PROTEZ TEMİZLİK ÜRÜNLERİNİN BAKTERİYOLOJİK AKTİVİTELERİNİN ARAŞTIRILMASI
AN INVESTIGATION OF THE BACTERIOLOGICAL ACTIVITY OF DENTURE CLEANSING PRODUCTS
Yazar: Akşit, Kazım; Nakipoğlu, Yaşar; Mandalı, Gamze; Günel, Gülşen; Gürler, Bülent
Dergi: Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, Vol. 25 No. 1, 2015
Anahtar kelimeler: Dentistry ; Diş Hekimliği ; Diş protezi, temizlik, dezenfeksiyon ; Denture, cleaning, disinfection

ÖZETAmaç: Diş protezlerinin temizliği ve dezenfeksiyonuna gereken önemin verilmemesi, ağız içi enfeksiyonlarla birlikte endokardit gibi sistemik enfeksiyonlara da yol açabilir. Bu tür enfeksiyonların önlenmesi veya azaltılabilmesi için uygun protez kaide materyalinin seçimi ile birlikte protezlerin temizlenmesi ve dezenfeksiyonunu sağlayabilen etkin bir ürünün belirlenmesi büyük önem taşımaktadır. Araştırma- mızda, halen Türkiye’de diş protezlerinin temizliği ve dezenfeksiyonlarında kullanılan çeşitli ürünlerin antibakteriyel etkinliklerinin araştırılması amaçlanmıştır.Materyal ve Metod: Araştırmamızda, 3 adet alkalen peroksit grubu efervesan diş protez temizleme tableti (Fittydent (Matilek-Avusturya), Corega tabs (GlaxoSmithKline, İrlanda), Protefix (Queisser pharma, Almanya)) ile 2 adet dezenfektan solüsyonun (Curaprox BDC 105 (haftalık kullanımlı) (Swiss Premium Oral Care, İsveç), Curaprox BDC 100 (Günlük kullanımlı) (Swiss Premium Oral Care, İsveç)); dört tip protez kaide materyali deney örneklerine (Vertex (Vertex-Dental B.V, Hollanda), Rodex (Rodont, İtalya), Meliodent (Kulzer, Almanya), Molloplast-B (Detax, Almanya)) bulaştırılmış Staphylococcus aureus ATCC 6538, Pseudomo- nas aeruginosa NCTC 6749 ve Bacillus subtilis var. niger ATCC 9372 bakteri suşlarına karşı etkinlikleri üretici firma tarafından önerilen sürelerde incelenmiştir.Bulgular: Denenen ürünlerin tamamının S.aureus ATCC 6538 bakteriye karşı; %100 oranında etkili olduğu belirlenmiştir. P.aeruginosa NCTC 6749 suşuna karşı ise; Fittydent’in, 30 dakika temas süresinde, Corega tabs ve Protefix tabletlerinin 15’er dakikada, Curaprox BDC 105’in ise, 1. ve 7.gün sonunda da 6 saatlik  temas süresinde aynı etkiyi (%100) gösterdiği belirtilmiştir. Curaprox BDC 100’ün ise, 5 dakika temas süresinde % 93-100 arasında etkinlik sağladığı gözlenmiştir. B.subtilis var. niger ATCC 9372 bakteri sporu üzerinde; Curaprox BDC 105’in 1. ve 7.gündeki solüsyonla- rının %99 oranında etkili olduğu, diğer ürünlerin ise temas süre ve protez maddelerine göre Fittydent’in; %97-98, Corega tabs’ ın ; %91-99, Protefix’in; %68-95, Curaprox BDC 100’ ün; %72-99 gibi değişen oranlarda dezenfeksiyon sağladığı belirlenmiştir. Sonuç: Farklı tip diş protez kaide maddeleri üzerinde temizlik ve dezenfeksiyon ürünlerinin etkinliklerinin ortamda bulunan bakterilere göre çok değişken olduğu, protez kaide maddesi tipinin ise dezenfeksiyon üzerinde bir etkisinin olmadığı gözlenmiştir. Protezlerin her gün temizlenmesinin ve dezenfeksiyonunun potansiyel patojen bakteriyel kontaminasyonunu önlemek için etkili bir yöntem olduğu sonucuna varılmıştır.Anahtar sözcükler: Diş protezi, temizlik, dezenfeksiyonAN INVESTIGATION OF THE BACTERIOLOGICAL ACTIVITY OF DENTURE CLEANSING PRODUCTSABSTRACTAim: The unsufficient cleaning and disenfection of the dentures not only lead to intra-oral infections but also to systemic infections such as endocarditis, which might be life threatening. To prevent or reduce such infections, selection of appropriate denture base material and effective denture cleaning or disinfecting preparations are very important. In our study, we aimed to investigate the antibacterial activity of cleansing and disinfectant preparations currently use in Turkey.Material and Method: In our study, the antibacterial activity of 3 alkaline peroxide type cleaning denture effervescent tablets (Fittydent (Matilek-Austria), Corega tabs (GlaxoSmithKline, Ireland), Protefix (Queisser pharma, Germany) frequently use in our country and 2 disinfectant solutions (Curaprox BDC 105 (weekly use) (Swiss Premium Oral Care, Sweden), Curaprox BDC 100 (daily use) (Swiss Premium Oral Care, Sweden), were tested against four  types of denture base material samples (Vertex (Vertex-Dental B.V, Netherlands), Rodex (Rodont, Italy), Meliodent (Kulzer, Germany), Molloplast-B (Detax, Germany)) contaminated with Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa NCTC 6749 and Bacillus subtilis var. niger ATCC 9372 at the contact times advised by the manufacturers. Result: It was determined  that all of the preparations were found to be 100% effective against S.aureus ATCC 6538 strain. Fittydent at 30 minutes, Corega tabs and Protefix  tablets at 15 minutes of each, and Curaprox BDC 105 at 6 hours in the solutions prepared in the 1.st and the end of the 7.th days, were have the same activity(100%) against P.aeruginosa NCTC 6749 strain.Otherwise, Curaprox BDC 100 at 5 minutes contact time was found 93-100 % effective against the same strain. The sporicidal activity of the Curaprox BDC 105 against B.subtilis var. niger ATCC 9372 at 6 hours contact times in the solutions prepared in the 1.st and the end of the 7.th days, were found to be 99%, whereas the activity of the rest of the products were varied depending on the contact time and denture base material and differed between 97-98% of Fittydent ,91-99% of Corega tabs , 68-95% of Protefix, 72-99% of Curaprox BDC 100.Conclusion: The effect of denture base materials on the cleaning and disinfection were very variable according to the kinds of bacteria present in the test environment and have not shown to be differed depending on the type of denture base material. Daily cleaning or disinfection of the dentures are an effective way to prevent bacterial contamination with potential pathogens Key words: Denture, cleaning, disinfection

Aim: The unsufficient cleaning and disenfection of the dentures not only lead to intra-oral infections but also to systemic infections such as endocarditis, which might be life threatening. To prevent or reduce such infections, selection of appropriate denture base material and effective denture cleaning or disinfecting preparations are very important. In our study, we aimed to investigate the antibacterial activity of cleansing and disinfectant preparations currently use in Turkey. Material and Method: In our study, the antibacterial activity of 3 alkaline peroxide type cleaning denture effervescent tablets (Fittydent (Matilek-Austria), Corega tabs (GlaxoSmithKline, Ireland), Protefix (Queisser pharma, Germany) frequently use in our country and 2 disinfectant solutions (Curaprox BDC 105 (weekly use) (Swiss Premium Oral Care, Sweden), Curaprox BDC 100 (daily use) (Swiss Premium Oral Care, Sweden), were tested against four types of denture base material samples (Vertex (Vertex-Dental B.V, Netherlands), Rodex (Rodont, Italy), Meliodent (Kulzer, Germany), Molloplast-B (Detax, Germany)) contaminated with Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa NCTC 6749 and Bacillus subtilis var. niger ATCC 9372 at the contact times advised by the manufacturers. Result: It was determined that all of the preparations were found to be 100% effective against S.aureus ATCC 6538 strain. Fittydent at 30 minutes, Corega tabs and Protefix tablets at 15 minutes of each, and Curaprox BDC 105 at 6 hours in the solutions prepared in the 1.st and the end of the 7.th days, were have the same activity(100%) against P.aeruginosa NCTC 6749 strain.Otherwise, Curaprox BDC 100 at 5 minutes contact time was found 93-100 % effective against the same strain. The sporicidal activity of the Curaprox BDC 105 against B.subtilis var. niger ATCC 9372 at 6 hours contact times in the solutions prepared in the 1.st and the end of the 7.th days, were found to be 99%, whereas the activity of the rest of the products were varied depending on the contact time and denture base materialand differed between 97-98% of Fittydent ,91-99% of Corega tabs , 68-95% of Protefix, 72-99% of Curaprox BDC 100.  Conclusion: The effect of denture base materials on the cleaning and disinfection were very variable according to the kinds of bacteria present in the test environment and have not shown to be differed depending on the type of denture base material. Daily cleaning or disinfection of the dentures are an effective way to prevent bacterial contamination with potential pathogens