Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells
Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells
Yazar: Yavaş, Mehmet Cihan; Akpolat, Veysi; Deveci, Engin; Bilgin, Hakki Murat; Kaplan, İbrahim; Seker, Ugur; Yildiz, İsmail; Alkis, Esref; Celik, Mustafa Salih; Akdağ, Mehmet Zülküf
Dergi: Dicle Tıp Dergisi, Vol. 45 No. 4, 2018
Anahtar kelimeler: Melatonin,ganoderma,high voltage,electromagnetic field,spermatogonia,antioxidant

Objective: Purposeof the study was to research the effect of an electromagnetic field created bya high voltage line on the testes tissue and the serum biochemistry of Wistaralbino male rats. At the same time, in the study also examined the protectiveeffects of melatonin and ganoderma lucidum. Methods: Inthe study, 64 rats were divided into eight equal groups (n: 8). In experimentslasting 26 and 52 days, the following groups were used: High voltage (HV), HV +Ganoderma lucidum (GI), HV + Melatonin (MEL), and Sham-control. MEL (10 mg/kg)was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremelylow frequency electric (80.3 V/m) and magnetic fields (2.48 µT) were appliedfor eight hours per day. Results:The biochemical results of the study show an increase in total oxidant status (TOS)and oxidative stress index (OSI) level and a decrease in total antioxidantstatus (TAS) level in the 26 and 52 day high voltage groups, compared to thecontrol group. In the high voltage groups, the nitric oxide (NO) valueincreased with the increase of the exposure period. Testicular mass (p>0.05),TAS, TOS, OSI and NO (p<0.05) were found at 26 days of comparison. Testicularweight, TAS, TOS, OSI (p<0.05), and NO (p>0.05) were found in 52 daycomparisons. In routine histopathology data, we detected effects on both the 26and 52 day HV groups, such as degeneration in spermatic cells and fullstructural deterioration and increasing hyalinisation. We also detected an stepup in the ligament cell in the tubular field for the 52-day test groups. Inmelatonin added group, proliferating cell nuclear antigen (PCNA)-positive cellswere more active. TUNEL assay analysis showed that there was no significantdifference between the the 26 day experiment groups (p>0.05), but that therewas a significant dissimilarity between the 52 day experiment groups(p<0.05).Conclusions:In our study, the oxidative effect of created by a high voltage results inchanges in serum biochemistry of rats and a number of degenerativedeterioration in the histological structure of testes. Aslo, it is shown thatthe oxidative effect created by a high voltage has negative effects on the creationof spermatogonia. GI partly protects against these effects, while melatonin ismore effective in this regard.

Objective: Purposeof the study was to research the effect of an electromagnetic field created bya high voltage line on the testes tissue and the serum biochemistry of Wistaralbino male rats. At the same time, in the study also examined the protectiveeffects of melatonin and ganoderma lucidum. Methods: Inthe study, 64 rats were divided into eight equal groups (n: 8). In experimentslasting 26 and 52 days, the following groups were used: High voltage (HV), HV +Ganoderma lucidum (GI), HV + Melatonin (MEL), and Sham-control. MEL (10 mg/kg)was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremelylow frequency electric (80.3 V/m) and magnetic fields (2.48 µT) were appliedfor eight hours per day. Results:The biochemical results of the study show an increase in total oxidant status (TOS)and oxidative stress index (OSI) level and a decrease in total antioxidantstatus (TAS) level in the 26 and 52 day high voltage groups, compared to thecontrol group. In the high voltage groups, the nitric oxide (NO) valueincreased with the increase of the exposure period. Testicular mass (p>0.05),TAS, TOS, OSI and NO (p<0.05) were found at 26 days of comparison. Testicularweight, TAS, TOS, OSI (p<0.05), and NO (p>0.05) were found in 52 daycomparisons. In routine histopathology data, we detected effects on both the 26and 52 day HV groups, such as degeneration in spermatic cells and fullstructural deterioration and increasing hyalinisation. We also detected an stepup in the ligament cell in the tubular field for the 52-day test groups. Inmelatonin added group, proliferating cell nuclear antigen (PCNA)-positive cellswere more active. TUNEL assay analysis showed that there was no significantdifference between the the 26 day experiment groups (p>0.05), but that therewas a significant dissimilarity between the 52 day experiment groups(p<0.05).Conclusions:In our study, the oxidative effect of created by a high voltage results inchanges in serum biochemistry of rats and a number of degenerativedeterioration in the histological structure of testes. Aslo, it is shown thatthe oxidative effect created by a high voltage has negative effects on the creationof spermatogonia. GI partly protects against these effects, while melatonin ismore effective in this regard.