Klorheksidin diglukonatın insan periferal kan kültürlerinde antioksidan enzim seviyeleri üzerine etkilerinin incelenmesi
Klorheksidin diglukonatın insan periferal kan kültürlerinde antioksidan enzim seviyeleri üzerine etkilerinin incelenmesi
Yazar: Arabacı, Taner; Turkez, Hasan; Çiçek, Yasin; Geyikoğlu, Fatime; Tatar, Abdulgani; Keles, Sait; Saybak, Arif; Kızıldağ, Alper
Dergi: Cumhuriyet Dental Journal, Vol. 16 No. 3, 2012
Anahtar kelimeler: Chlorhexidine, toxicity, antioxidantenzyme. ; Klorheksidin, Toksisite, Antioksidan enzim

Objectives: In the present study, it was aimed to investigate the biochemical effects of chlorhexidine digluconate (CHX) on the antioxidant enzyme levels in human peripheral blood cell cultures.  Materials and Methods: The blood cultures were prepared using the blood samples obtained from 10 individuals (5 male and 5 female) who were systemically healthy and were not exposed to any toxic agent before. The cultures were exposed to different concentrations of CHX (0.05, 0.1, 0.2 ve 0.4 mmol/L). Glutation peroxidase (GPx), superoxide dismutase (SOD) and Catalase (CAT) enzyme activities were analyzed in order to evaluate the biochemical effects.  Results: A dose-dependent statistically significant reduction was seen in the GPx, SOD and CAT enzyme activities in the blood cultures treated with 0.1, 0.2 ve 0.4 mmol/L concentrations of CHX.  Conclusion: This is the first in vitro study investigating the effects of CHX on antioxidant enzyme levels in the human peripheral blood cultures. In conclusion, it was revealed that CHX had dose-depended cytotoxic effects by influencing the antioxidant enzyme activities in blood cells.  ÖZET Amaç: Bu çalışmada, klorheksidin diglukonatın (KHG) insan periferal kan kültürlerinde antioksidan enzim seviyeleri üzerine olan biyokimyasal etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Daha önce herhangi bir toksik ajana maruz kalmamış ve sistemik olarak sağlıklı 10 bireyden (5 erkek, 5 kadın) elde edilen kan örnekleri ile kan kültürleri hazırlandı. Elde edilen kültürler farklı konsantrasyonlarda KHG (0.05, 0.1, 0.2 ve 0.4 mmol/L) ile muamele edildi. Biyokimyasal etkilerin değerlendirilmesi amacıyla glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD) ve katalaz (KAT) enzim aktiviteleri incelendi. Bulgular: KHG'nin 0.1, 0.2 ve 0.4 mmol/L'luk konsantrasyonları ile muamele edilen kan kültürlerinde GPx, SOD ve CAT enzim aktivitelerinde doza bağlı istatistiksel olarak anlamlı derecede azalma izlendi (p<0.05). Sonuç: Bu çalışma, KHG'nin insan periferal kan kültürlerinde antioksidan enzim seviyeleri üzerindeki etkilerini araştıran ilk in vitro çalışmadır. Sonuç olarak, bu bileşiğin kan hücrelerindeki antioksidan enzim aktivitelerini etkilemek suretiyle doza bağlı sitotoksik etkilere sahip olduğu da ortaya konulmuştur.

Objectives: In the present study, it was aimedto investigate the biochemical effects of chlorhexidine digluconate (CHX) on the antioxidant enzyme levels in human peripheralblood cell cultures.Materials and Methods: The blood cultureswere prepared using the blood samples obtainedfrom 10 individuals (5 male and 5 female) whowere systemically healthy and were not exposed toany toxic agent before. The cultures were exposedto different concentrations of CHX (0.05, 0.1, 0.2ve 0.4 mmol/L). Glutation peroxidase (GPx),superoxide dismutase (SOD) and Catalase (CAT)enzyme activities were analyzed in order toevaluate the biochemical effects.Results:A dose-dependent statisticallysignificant reduction was seen in the GPx, SOD andCAT enzyme activities in the blood cultures treatedwith 0.1, 0.2 ve 0.4 mmol/L concentrations ofCHX. Conclusion: This is the first in vitro studyinvestigating the effects of CHX on antioxidantenzyme levels in the human peripheral bloodcultures. In conclusion, it was revealed that CHXhad dose-depended cytotoxic effects by influencingthe antioxidant enzyme activities in blood cells.