Şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etkisi
Şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etkisi
Yazar: Özpınar, Hülya; Dağ, Şeker; Yiğit, Emel
Dergi: Cumhuriyet Tıp Dergisi, Vol. 35 No. 2, 2013
Anahtar kelimeler: Persica vulgaris Miller, antibakteriyel etki, cut plug metot, methanol ekstraktı. ; Persica vulgaris Miller, antibacteriel effect, cut plug methot, methanol extract

Özet  Amaç. Çalışmamızda şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etkisini tespit etmek amaçlanmıştır. Yöntem. Erzincan Bahçe Bitkileri Araştırma Enstitüsü Gen Bahçesi’nde bulunan Cardinal çeşidi 7 adet şeftali (Persica vulgaris Miller) ağacı yaprağı methanol ekstraktı bu amaçla kullanılmıştır. Antibakteriyel etkisinin araştırılmasında cut plug yöntemi kullanılırken Minimum İnhibisyon Konsantrasyonu (MİK), mikrodilüsyon yöntemi ile belirlenmiştir. Bulgular. Bu çalışmada şeftali (Persica vulgaris Miller) yaprak ektraktının Staphylococcus aureus, Staphylococcus aureus (MRSA)’a karşı antibakteriyel etkisi belirlenemezken, Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus ,Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Yersinia enterocolitica bakterilerine karşı ise antibakteriyel etki tespit edilmiştir. En düşük Minimum İnhibisyon Konsantrasyonu Pseudomonas aeruginosa ve Yersinia enterocolitica’da tespit edilirken (0,01 mg/mL), en yüksek konsantrasyon Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes’de görülmüştür (10 mg/mL). Sonuç. Çalışmamız sonucunda şeftali (Persica vulgaris Miller) yaprak ekstraktının antibakteriyel etki spektrumu invitro olarak tespit edilmiş olup bu tür bitkilerden yola çıkılarak yeni antibakteriyel ajanların oluşum sürecinin başlatılabileceği düşünülmektedir. Anahtar sözcükler: Persica vulgaris Miller, antibakteriyel etki, cut plug metot, methanol ekstraktı.   Abstract Aim. In our study we aimed to determine antibacterial effect of peach leaves extract. Methods. In Erzincan Horticultural Research Institute Gen Garden varietes seven cardinal peach’s (Persica vulgaris Miller (Rosaceae) tree leaf methanol extract was used for this purpose, Antibacterial effect was evaluated by cutplug method whereas minimum inhibtory concentration (MIC) was determined by microdilution method. Results. In this study peach’s (Persica vulgaris Miller ) leaf extract antibacterial effect on Staphylococcus aureus, Staphylococcus aureus (MRSA) was not determined, on the other hand the antibacterial effects on Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Yersinia enterocolitica was determined. The lowest minimum inhibitory concentration on Pseudomonas aeruginosa and Yersinia enterocolitica detected as (0.01 mg/mL), the higest concentration on grup of Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes was determined as (10 mg/mL). Conclusions. As a result of our study peach’s (Persica vulgaris Miller) leaves extract spectrum showed broad antibacterial effect. Starting from such plants, thought to be started the process of the formation of new antibacterial agents. Keywords: Persica vulgaris Miller, antibacteriel effect, cut plug methot, methanol extract

Aim. In our study we aimed to determine antibacterial effect of peach leaves extract. Methods. In Erzincan Horticultural Research Institute Gen Garden varietes seven cardinal peach’s (Persica vulgaris Miller (Rosaceae) tree leaf methanol extract was used for this purpose, Antibacterial effect was evaluated by cutplug method whereas minimum inhibtory concentration (MIC) was determined by microdilution method. Results. In this study peach’s (Persica vulgaris Miller ) leaf extract antibacterial effect on Staphylococcus aureus, Staphylococcus aureus (MRSA) was not determined, on the other hand the antibacterial effects on Escherichia coli, Escherichia coli O:157 H:7, Proteus mirabilis, Proteus vulgaris, Salmonella enteritidis, Salmonella typhimurium, Streptococcus pyogenes, Enterococcus faecalis, Staphylococcus epidermidis, Klebsiella pneumoniae, Listeria monocytogenes, Micrococcus luteus, Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Yersinia enterocolitica was determined. The lowest minimum inhibitory concentration on Pseudomonas aeruginosa and Yersinia enterocolitica detected as (0.01 mg/mL), the higest concentration on grup of Bacillus subtilis, Escherichia coli, Escherichia coli O:157 H:7, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus epidermidis, Streptococcus pyogenes was determined as (10 mg/mL). Conclusions. As a result of our study peach’s (Persica vulgaris Miller) leaves extract spectrum showed broad antibacterial effect. Starting from such plants, thought to be started the process of the formation of new antibacterial agents.