Çeşitli kanserli dokularda tromboplastik aktivite ve protein dağılımlarının poli akrilamid jel elektroforezi ile incelenmesi
Investigation of thromboplastic activity and protein distribution (SDS-PAGE) in different patients with cancer
Yazar: TÜLAY UÇAR
Danışman: PROF.DR. NESRİN EMEKLİ
Marmara Üniversitesi / Sağlık Bilimleri Enstitüsü / Biyokimya Ana Bilim Dalı
Derece: Yüksek Lisans, 2006
Konu: Biyokimya = Biochemistry

Özet:
g=(77URPER] YH NDQVHU DUDVÃQGD ELU LOLÃºNL ROGXÃ·X X]XQ ]DPDQGDQ EHUL ELOLQPHVLQH UDÃ·PHQ EXLOLÃºNL KDNNÃQGD ELOJLOHULPL] JÂQÂPÂ]GH KDOD QHWOHÃºPHPLÃºWLU KHPRVWD]ÃQ EX NRQXGDNL |QHPLWDUWÃÃºÃOPDNWD YH GRNX IDNW|UÂQÂQ NDQVHU JHOLÃºLPLQGH GH |QHPOL ROGXÃ·X ELOGLULOPHNWHGLU %XoDOÃÃºPDQÃQ DPDFÃ NDQVHU WXWXOXPX RODQ YH ROPD\DQ oHÃºLWOL GRNXODUGD WURPERSODVWLN DNWLYLWH\LLQFHOHPHN EX GRNXODUÃQ SURWHLQ GDÃ·ÃOÃPODUÃQÃ 6'63$*( LOH J|UPHN YH D\QÃ KDVWDODUÃQNDQODUÃQGD SURWURPELQ ]DPDQÃ IDNW|U 9,,D ILEULQRMHQ YH $377 JLEL KHPRVWDWLNSDUDPHWUHOHULQ GHÃ·LÃºLPOHULQL J|UPHNWLUdDOÃÃºPDGD Ã¹LÃºOL (WIDO (Ã·LWLP YH $UDÃºWÃUPD +DVWDQHVLQGH FHUUDKL UH]HNVL\RQOD DOÃQÃS SDWRORMLNOLQLÃ·LQH J|QGHULOPLÃº RODQ oHÃºLWOL NDQVHUOL GRNXODU NXOODQÃOGÃ 3DWRORML NOLQLÃ·LQGH D\ÃUÃPÃ\DSÃODQ GRNXODUÃQ KRPRMHQL]DV\RQX \DSÃOGÃ dDOÃÃºPD JUXEXPX]X ROXÃºWXUDQ WRSODPKDVWDGDQ HÃºOHÃºWLUHELOGLÃ·LPL] oHÃºLWOL NDQVHUOL GRNX LOH D\QÃ GRNXODUÃQ NDQVHU WXWXOXPXROPD\DQ E|OJHOHULQH DLW WURPERSODVWLN DNWLYLWHOHUL DUDVÃQGD LOHUL GHUHFHGH DQODPOÃ IDUNYDUGÃU S %D]Ã NRDJXODV\RQ SDUDPHWUHOHUL LOH PLGH NRORQ PHPH NDQVHUL DUDVÃQGDLOLÃºNLOL ROGXÃ·XQX J|UGÂN %X NRUHODV\RQODU YH DQODPOÃOÃNODUÃ VÃUDVÃ\OD Ãº|\OHGLU UU S U U U U S S U UU S.DQVHUOL GRNXODUÃQ ED]ÃODUÃQGD WURPERSODVWLN DNWLYLWH DUWPÃÃºWÃU $\QÃ KDVWDODUÃQ KHPRVWDWLNSDUDPHWUHOHULQGH EHQ]HU ELU DQODPOÃOÃN WHVSLW HGLOHPHPLÃºWLU

Abstract:
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Ã¹LÃº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ö