Mangan ve demir stresi uygulanmış roka (Eruca sativa) bitkisinde bazı antioksidan enzim aktivitesindeki ve lipid peroksidasyon düzeylerindeki değişimlerin incelenmesi
Investigation of some antioxidant activity and lipid peroxidation various in roca (Eruca sativa) treated with manganase and iron stress
Yazar: SEDA ÇINAR
Danışman: PROF. DR. TÜLİN AYDEMİR
Celal Bayar Üniversitesi / Fen Bilimleri Enstitüsü / Kimya Ana Bilim Dalı
Derece: Yüksek Lisans, 2005
Konu: Kimya = Chemistry

Özet:
ÖZETYedi gün çimlendirilmi roka (Eruca sativa) bitkisi 16 saat k- 8 saat karanl k büyümeçemberinde 0-5000 ÂµM aras nda de i en Mn+2 ve Fe+2 içeren besi ortam nda strese maruzb rak ld . Mangan toksisitesi uygulanan roka yapraklar nda kontrol grubuna göre süperoksitdismutaz (p 0.05), guasikol peroksidaz (p 0.01) aktiviteleri ve lipid peroksidasyon seviyeleri(p 0.05) daha yüksek, askorbat peroksidaz aktivitesi (p 0.05) ise daha dü ük bulundu. Artanmangan konsantrasyonuna ba l olarak yapraklardaki klorofil içeri inde azalma gözlendi(p 0.01). Stres uygulanan roka yapraklar n n 6. gününde yapraklarda kahverengibeneklenmeler, relatif büyüme h z nda ve gövde (yaprak+sap) uzunlu unda azalmalarbelirlendi.15 günlük muamele periyodunda 3.45-5000 ÂµM aral nda mangan toksisitesiuygulanan roka köklerinde ise kontrol grubuna göre Mn art na ba l olarak süperoksitdismutaz (p 0.05), askorbat peroksidaz (p 0.05), guasikol peroksidaz (p 0.01) aktivitelerindeve lipid peroksidasyon seviyelerinde (p 0.05) artma saptand . Bitki büyüme parametreleriincelendi inde ise roka bitkisinin kök uzunluklar nda artan mangan konsantrasyonuna ba lolarak azalmalar gözlendi.0-3.45 ÂµM mangan eksiklik stresi uygulanm roka yapraklar nda 15. günün sonundakontrol grubuna göre süperoksit dismutaz (p 0.01), guasikol peroksidaz (p 0.01)aktivitelerinin ve lipid peroksidasyon düzeylerinin (p 0.05) artt , askorbat peroksidazaktivitesinin (p 0.05) ve klorofil içeri inin azald belirlendi. Mn eksikli i uygulanan rokayapraklar nda 6. günün sonunda relatif büyüme h z nda ve gövde (yaprak+sap) uzunlu undas ras yla % 2.15 ve %10 azalma ve yapraklarda sararmalar gözlendi.Fe+2 toksisitesi uygulanm roka yapraklar nda 15. gün sonunda kontrol grubuna göreyapraklardaki süperoksit dismutaz (p 0.01), askorbat peroksidaz (p 0.05), guasikolperoksidaz (p 0.05) aktiviteleri ve lipid peroksidasyon seviyelerinin (p 0.05) belirgin olarakartt belirlendi.15 günlük muamele periyodunda artan demir konsantrasyonuna ba l olarakklorofil içeri inde(p 0.05) azalmalar belirlendi. 6. günde relatif büyüme h z nda ve gövde(yaprak+sap) uzunlu unda azalmalar ve gözlendi.Artan Fe+2 konsantrasyonuna ba l olarak stres uygulanan roka köklerinin süperoksitdismutaz (p 0.01), guasikol peroksidaz aktivitesi (p 0.05) ve lipid peroksidasyon düzeyleri(p 0.01) kontrol grubuna göre artt , askorbat peroksidaz (p 0.05) aktivitesinin azaldbelirlendi. Roka bitkisinin kök uzunluklar n n kontrol grubuna göre azald ve köklerdesaçaklanmalar n oldu u gözlendi.0-34.7 ÂµM Fe+2 aral nda 15 gün eksiklik stresi uygulanan roka yapraklar nda,kontrol grubuna göre süperoksit dismutaz (p 0.05) ve lipid peroksidasyon düzeylerinde(p 0.01) artma askorbat peroksidaz ve guasikol peroksidaz aktivitelerinde ise kontrolgrubuna göre azalmalar gözlendi (p 0.05) Demir eksikli i uygulanm roka yapraklar ndakontrol grubuna göre toplam klorofil içeri inde % 31 oran nda (p 0.01) azalma gözlendi. 6.günde demir miktar n n azal na ba l olarak yapraklarda sararmalar, büyümeparametrelerinde % 15 oran nda azalma belirlendi.Anahtar Kelimeler: Antioksidan enzimler, lipid peroksidasyonu, metal stresi, oksidatif stres,roka (Eruca sativa)

Abstract:
ABSTRACTThe roca plants (Eruca Sativa) germinated for eight days were exposed to Mn2+ and2+Fe stresses in changing concentration between 0-5000 M in 16 hours light 8 hours darkconditioned growing chamber. The activities of superoxide dismutase (p<0.05), guaiacolperoxidase (p<0.05) and lipid peroxidation levels were found higher in the roca leavesstressed with manganese toxicity than the control group, but ascorbate peroxidase activitywas lower. A decrease in chlorophyll content in the leaves was detected according to theincreased manganese concentration (p<0.01). Brownish spots were noticed on roca leavesafter six days applied stress, and decreases in relative growing rate and body length(leave+stem).An increase in activities of superoxide dismutase (p<0.05), ascorbate peroxidase(p<0.05) guaiacol peroxidase (p<).05) and lipid peroxidation levels (p<0.05) was observed inthe roca rots exposed to manganese toxicity between 3.45-5000 M concentration range inaccordance with manganese toxicity after 15 days treatment period. When the plant growthparameters were investigated, a decrease was observed in rots length of roca plant with theincreased concentration of manganese.After 15 days period, an increase in activities of superoxide dismutase (p<0.01),guaiacol peroxidase (p<0.01) and lipid peroxidation levels (p<0.05) and a decrease in activityof ascorbate peroxidase (p<0.05) and content of chlorophyll were detected in the roca leavesexposed to manganese deficiency between 0-3.45 M concentration rang at the end of 15days when it was compared with the control group. A decrease of 2.15% in relative growthrate and 10% in body length (leaves + stem) was observed when the roca leaves exposed tomanganese deficiency for 6 days.A significant increase in the activities of superoxide dismutase (p<0.01), ascorbateperoxidase (p<0.05), guaiacol peroxidase (p<0.05) and lipid peroxidation levels wasobserved in the roca leaves exposed to Fe2+ toxicity at the end of 15 days when it wascompared with the control group. A decrease in content of chlorophyll (p<0.05) was detectedin 15 days treatment periods in accordance with iron concentration. In 6 days period adecrease in relative growth rate and body length (leaves+stem) was observed.In the roca rots stressed with the increased Fe2+ concentration, an increase in theactivities of superoxide dismutase (p<0.01), guaiacol peroxidase (p<0.05) and lipidperoxidation levels and a decrease in activity of ascorbate peroxidase (p<0.05) weredetected when it was compared with the control group. A decrease in length of the roca plantrots and branches in rots were detected when it was compared with the control groups.An increase in the levels of superoxide dismutase (p<0.05) and lipid peroksidasyonbut a decrease in the activities of ascorbate peroxidase and guaiacol peroxidase wereobserved in roca leaves exposed to Fe2+ deficiency between 0-34.7 M concentration rangefor 15 days when it was compared with the control group. A decrease of 31% in totalchlorophyll content (p<0.01) was detected in roca leaves exposed to iron deficiency when itwas compared to the control group. According to decreasing iron concentration, a yellowishcolour and a decrease of 15% in growing parameters were detected after 6 days.Key Words. Antioxidan enzymes, lipid peroxidation, metal stress, oxidative stress, roca(Eruca sativa).