Jelatin ve histon kullanılarak doku mühendisliği uygulamaları için yüksek biyouyumlu biyomalzeme sentezi ve karakterizasyonu
Highly biocompatible biomaterials synthesis and characterization for tissue engineering applications by using gelatin and histone
Yazar: SABİHA DEMİRCİ CENGİZ
Danışman: PROF. DR. FATMA AYHAN
Muğla Sıtkı Koçman Üniversitesi / Fen Bilimleri Enstitüsü / Kimya Ana Bilim Dalı
Derece: Doktora, 2024
Konu: Kimya = Chemistry

Özet:
Hydrogels are cross-linked, three-dimensional, water-swellable polymers. Hydrogels, which have a variety of production methods for controllable biodegradation and mechanical properties, are used in many application areas such as biomedical fields. In tissue engineering studies, which is one of the biomedical applications, it is important to synthesize and use hydrogels with desired properties and to investigate their cytotoxic effects. 
In this study; high molecular weight polyethylene glycol diacrylate (PEGDA), gelatin methacryloyl (GELMA) and histone methacryloyl (HISMA) hydrogels with different pore structures, high water and protein content, swellable in water, with different properties were synthesized and cytotoxicity studies of hydrogels were performed for their applicability in tissue engineering.
PEGDA was synthesized from high molecular weight polyethylene glycol (PEG) and characterized using ATR-FTIR, DSC and 1H-NMR methods. Gelatin methacryloyl (GELMA) was synthesized based on type A and type B gelatin. The degree of substitution of the synthesized GELMAs was determined by TNBS analysis, characterized using ATR-FTIR and 1H-NMR devices and the structure was determined. Using histone protein, histone methacryloyl (HISMA) was synthesized by modifying its structure. The structure of the synthesized HISMA was determined by ATR-FTIR and TNBS analysis and HISMA was characterized. 
Hydrogels were synthesized using the synthesized PEGDA and GELMA macromers. The synthesized hydrogels were characterized by ATR-FTIR, DSC analysis, SEM analysis, mechanical analysis and dynamic swelling behavior and biodegradation behavior. In order to explain the applicability of the hydrogels in tissue engineering studies, their interactions with L929 fibroblast cells were examined and it was investigated whether they showed cytotoxic effects on the cells. In addition, the attachment, proliferation and growth behaviors of the cells to the hydrogel structures synthesized with L929 fibroblast cells were examined in vitro.
In the study; histone was modified and characterized using a different method than the one in the literature. In addition, all synthesized hydrogels were found to be biocompatible and can be used as cell tissue scaffolds in tissue engineering applications.

Abstract:
Hydrogels are cross-linked, three-dimensional, water-swellable polymers. Hydrogels, which have a variety of production methods for controllable biodegradation and mechanical properties, are used in many application areas such as biomedical fields. In tissue engineering studies, which is one of the biomedical applications, it is important to synthesize and use hydrogels with desired properties and to investigate their cytotoxic effects. 
In this study; high molecular weight polyethylene glycol diacrylate (PEGDA), gelatin methacryloyl (GELMA) and histone methacryloyl (HISMA) hydrogels with different pore structures, high water and protein content, swellable in water, with different properties were synthesized and cytotoxicity studies of hydrogels were performed for their applicability in tissue engineering.
PEGDA was synthesized from high molecular weight polyethylene glycol (PEG) and characterized using ATR-FTIR, DSC and 1H-NMR methods. Gelatin methacryloyl (GELMA) was synthesized based on type A and type B gelatin. The degree of substitution of the synthesized GELMAs was determined by TNBS analysis, characterized using ATR-FTIR and 1H-NMR devices and the structure was determined. Using histone protein, histone methacryloyl (HISMA) was synthesized by modifying its structure. The structure of the synthesized HISMA was determined by ATR-FTIR and TNBS analysis and HISMA was characterized. 
Hydrogels were synthesized using the synthesized PEGDA and GELMA macromers. The synthesized hydrogels were characterized by ATR-FTIR, DSC analysis, SEM analysis, mechanical analysis and dynamic swelling behavior and biodegradation behavior. In order to explain the applicability of the hydrogels in tissue engineering studies, their interactions with L929 fibroblast cells were examined and it was investigated whether they showed cytotoxic effects on the cells. In addition, the attachment, proliferation and growth behaviors of the cells to the hydrogel structures synthesized with L929 fibroblast cells were examined in vitro.
In the study; histone was modified and characterized using a different method than the one in the literature. In addition, all synthesized hydrogels were found to be biocompatible and can be used as cell tissue scaffolds in tissue engineering applications.