Böbrek iskemi-reperfüzyon hasarında iloprostun koruyucu etkisi
The prorective effect of iloprost in renal ischemia/reperfusion injury
Yazar: KORAY AYKUT
Danışman: DOÇ. DR. EYÜP HAZAN
Dokuz Eylül Üniversitesi / Tıp Fakültesi / Kalp ve Damar Cerrahisi Ana Bilim Dalı
Derece: Tıpta Uzmanlık, 2005
Konu: Kardiyoloji = Cardiology

Özet:
1.2 SUMMARY The Protective Effect of Iloprost in Renal Ischemia/Reperfusion Injury Temporary interruption of tissue oxygenation followed by reperfusion leads to a cascade of events that cause cell and tissue death by disintegrating cell membrane and defunctionalizing cell organelles. Neutrophils and oxygen free radicals have the main role in this process which is called ischemia/reperfusion injury. In this study, effect of iloprost in renal ischemia/reperfusion is investigated on an experimental animal model. Adult, male Wistar rats subjected to 60 minutes of unilateral warm ischemia were seperated into sham (n=4), control (n=7) and iloprost (n=7) groups, iloprost was administered by right internal juguler vein at a dose of 2 ng/kg"Vmin l. Infusion started with the same time as we clamped the artery of the left kidney and continued for about an hour in the early phase of reperfusion. Blood samples for biochemical parameters were taken just before clamping the renal artery and post operative 72n hour. Histopathological examinations of the kidney specimens were made to detect probable changes due to reperfusion injury. We used a tight microscope to examine the specimens. Mean BUN levels after 72 hours were 181,9 ± 108,2 mg/dl for the control group and 127,5 ± 70,3 for iloprost group. (p=0,383) Mean Kr levels after 72 hours were 5,0 ± 3,1 for the control group, 3,7 ± 1,7 for iloprost group. (p=0.456) Mean BUN and Kr levels in iloprost group were less than the levels in the control group, but the difference between two groups was not statistically significant. Serious (Grade 2 or Grade 3) acute tubuler necrosis was detected in 86 % of rats in control group (6/7), the ratio was 57 % (4/7) in the latter group. But the difference between two groups was not statistically significant (p=0.209) Although these findings suggest that, iloprost pretreatment in rat experimental ischemia/reperfusion model does not statistically reduce injury, there is a trend in iloprost group to have less injury due to ischemia and reperfusion.We think that the number of the rats in each group were not enough to make a statistical difference and more studies are needed to show the beneficial effects of iloprost in ischemia/reperfusion injury of the kidney. Key words : iloprost, ischemia, reperfusion injury, kidney

Abstract:
1.2 SUMMARY The Protective Effect of Iloprost in Renal Ischemia/Reperfusion Injury Temporary interruption of tissue oxygenation followed by reperfusion leads to a cascade of events that cause cell and tissue death by disintegrating cell membrane and defunctionalizing cell organelles. Neutrophils and oxygen free radicals have the main role in this process which is called ischemia/reperfusion injury. In this study, effect of iloprost in renal ischemia/reperfusion is investigated on an experimental animal model. Adult, male Wistar rats subjected to 60 minutes of unilateral warm ischemia were seperated into sham (n=4), control (n=7) and iloprost (n=7) groups, iloprost was administered by right internal juguler vein at a dose of 2 ng/kg"Vmin l. Infusion started with the same time as we clamped the artery of the left kidney and continued for about an hour in the early phase of reperfusion. Blood samples for biochemical parameters were taken just before clamping the renal artery and post operative 72n hour. Histopathological examinations of the kidney specimens were made to detect probable changes due to reperfusion injury. We used a tight microscope to examine the specimens. Mean BUN levels after 72 hours were 181,9 ± 108,2 mg/dl for the control group and 127,5 ± 70,3 for iloprost group. (p=0,383) Mean Kr levels after 72 hours were 5,0 ± 3,1 for the control group, 3,7 ± 1,7 for iloprost group. (p=0.456) Mean BUN and Kr levels in iloprost group were less than the levels in the control group, but the difference between two groups was not statistically significant. Serious (Grade 2 or Grade 3) acute tubuler necrosis was detected in 86 % of rats in control group (6/7), the ratio was 57 % (4/7) in the latter group. But the difference between two groups was not statistically significant (p=0.209) Although these findings suggest that, iloprost pretreatment in rat experimental ischemia/reperfusion model does not statistically reduce injury, there is a trend in iloprost group to have less injury due to ischemia and reperfusion.We think that the number of the rats in each group were not enough to make a statistical difference and more studies are needed to show the beneficial effects of iloprost in ischemia/reperfusion injury of the kidney. Key words : iloprost, ischemia, reperfusion injury, kidney