Oleokantalin nöroblastoma hücresinde oksidatif stres ve apoptoz üzerine  etkisinin araştırılması /
Yazar: ÜLKÜ ÜNLÜ ÜNSAL
Danışman: YRD. DOÇ. DR. MESUT METE
Celal Bayar Üniversitesi / Tıp Fakültesi / Beyin ve Sinir Cerrahisi Ana Bilim Dalı
Derece: Tıpta Uzmanlık, 2015
Konu: Histoloji ve Embriyoloji = Histology and Embryology ; Nöroşirürji = Neurosurgery
Dizin: Apoptozis = Apoptosis ; Merkez sinir sistemi neoplazmları = Central nervous system neoplasms ; Neoplazmlar = Neoplasms ; Nöroblastom = Neuroblastoma ; Oksidatif stres = Oxidative stress

Özet:
Nöroblastom (NB) genellikle sporadik olarak ortaya çıkan , çocukluk döneminin en sık görülen ekstrakraniyal tümördür. Pediatrik kanser tedavisindeki gelişmelere rağmen 
(Cerrahi,kemoterapi, radyoterapi) yaşama oranı %40'ların altında kalmaktadır. Bu nedenle 
diğer santral sinir sistemi tümörlerinde olduğu gibi nöroblastomada da farklı tedavi 
Nöroblastoma da diğer birçok tümörde olduğu gibi, apopitoz tümör gelişimi için 
önemli bir mekanizmadır ve bu konuda çeşitli çalışmalar yapılmıştır.
Yapmış olduğumuz çalışmada,sızma zeytinyağından elde edilen bir fenolik 
komponent olan oleokantalin kültür ortamındaki nöroblastoma kanser hücreleri üzerine hücre 
canlılığı, oksidatif stres, nörit inhibisyonu ve apoptotik etkilerini araştırdık. Oleokantal maddesinin nöroblastoma kanser hücrelerini doz bağımlı olarak etkilediği ve IC50 dozunda her dört etkinin de daha belirgin olarak saptandığı gözlemlendi.

Abstract:
Aim: Neuroblastoma accounts for %15 of all children's age tumoral mortality causes. 
Prognosis may be an issue due to changeable biological behaviour and aggresive process. 
Apoptosis is an important mechanism in neuroblastoma as is in many other tumors and many 
researches were published implying it. Neuroblastoma cells have been used as an in vitro 
model in antitumoral mechanisms  such as stopping the cell cycle, inducing apopitosis, 
reducing n-myc expression. The aim of this study is to show the effects of oleocanthal on in vitro  neuroblastoma cells.
Material and Method: Cells were divided in two groups. Group 1: the neuroblastoma cells 
treated with oleocanthal. Group 2: Neuronal stem cells treated with oleocanthal. The effects on cell viability, oxidative stres, neuritis inhibition and apoptosis of olecanthal at dose of IC50 Results: In MTT analysis, used for detecting cell viability, in Group 1 the cells were less 5 viable compared with Group 2, and the difference was statistically significant (p<0,001). In group 1, the rates of I-NOS and E-NOS stains used for analysis of oxidative stress were greater than group 2, and the difference was statistically significant (p<0,005). In neurotoxicity screening test analysis used for neuritis inhibition, it was shown that oleocanthal pperently inhibited neuritis growing in neuroblastoma cells, while in bone marrow derived neurons it was less inhibitive, and the difference was statistically significant. (p<0,001). TUNEL stain to determine cell apoptosis in Group 1  was greater than Group 2 and also the difference was statistically significant(p<0,005).
Conclusion: The effects of oleocanthal was showed out in vitro on neuroblastoma cells. The 
apoptotic effect of oleocanthal on neuroblastoma cells was detected and shown by TUNEL 
assay, and it is considered to be a potential monotherapy or combined therapy with other 
cytotoxic medicine used in oleocanthal based chemotherapies in the future.