Boğaz/nazofarinkste kolonize olan neisseria türlerinin saptanması ve neisseria meningitidis suşlarında penisilin direncinin aranması
Isolation of neisseria strains which colonized throat/nasopharynx and detection of penicillin resistance of neisseria meningitidis strains
Yazar: ZEHRA ÇEKİÇ
Danışman: PROF.DR. BETİGÜL ÖNGEN
İstanbul Üniversitesi / Sağlık Bilimleri Enstitüsü / Mikrobiyoloji ve Klinik Mikrobiyoloji Ana Bilim Dalı
Derece: Yüksek Lisans, 2006
Konu: Mikrobiyoloji = Microbiology

Özet:
ÖZETN. meningitidis JHOLÃºPLÃº YH JHOLÃºPHNWH RODQ ÂONHOHUGH HQGHPLN YH HSLGHPLNinfeksiyonlara neGHQ ROXU 0HQLQJRNRN LQIHNVL\RQX LQVLGDQVÃQÃQ EDWÃ ÂONHOHULQGH RNXOoDÃ·Ã |QFHVL oRFXNODUGD GDKD \ÂNVHN ROGXÃ·X VDSWDQPÃÃºWÃU N. meningitidis'inQD]RIDULQNVWH WDÃºÃ\ÃFÃOÃÃ·ÃQÃQ SDWRJHQH]GH LON DGÃP ROGXÃ·X GÂÃºÂQÂOPHNWHGLUdurum, toplumda geçirilen menenjit7DÃºÃ\ÃFÃOÃN RUDQODUÃQÃ \DÃº VRV\RHNRQRmikHQIHNVL\RQXQXQVÃNOÃÃ·ÃJLELELUoRNIDNW|UHWNLOHPHNWHGLUNeisseria$UDÃºWÃUPDFÃODU ÂVW VROXQXP \ROXQGD NRORQL]H RODQ NRPPHQVDOtürlerinin N. meningitidis LQIHNVL\RQXQD NDUÃºÃ NRUX\XFX DQWLNRUODU JHOLÃºWLUGLNOHULQLGÂÃºünmektedirler.N. meningitidis'in penisiline orta ve6RQ \ÃOODUGD GÂQ\DQÃQ ELUoRN ÂONHVLQGHNeisseria türlerinin de\ÂNVHN GÂ]H\GH GLUHQo J|VWHUGLÃ·L VDSWDQPÃÃºWÃU .RPPHQVDON. meningitidis ile birlikteoRÃ·XQXQ SHQLVLOLQH GLUHQo JHQLQL WDÃºÃGÃÃ·Ã YHbulunduNODUÃQGD SHQLVLOLQH GLUHQo JHQLQL N. meningitidis'e transfer ettikleri ilerisürülmektedir. Bu nedenle nazofarinkste kolonize olan kommensal NeisseriaWÂUOHULQLQ EHOLUOHQPHVL YH SHQLVLOLQH GLUHQo RUDQODUÃQÃQ VDSWDQPDVÃ |QHPND]DQPDNWDGÃUÃ¸VWDQEXOÂ¶XQ IDUNOÃ VHPWOHULQGH EXOXQDQ EHÃº NUHÃºWHNL oRFXÃ·XQ ERÃ·D]VÂUÂQWÂVÂ |UQHNOHUL Ã¸VWDQEXO hQLYHUVLWHVL Ã¸VWDQEXO 7ÃS IDNÂOWHVL 0LNUREL\RORML YH .OLQLNNeisseria FLQVL EDNWHUL VXÃºX L]ROH0LNUREL\RORML ODERUDWXDUODUÃQGD oDOÃÃºÃOPÃÃº YHHGLOPLÃºWLU dRFXNODUGD HQ IDzla Neisseria subflava biovar perflava (%33), en az N.cinerea NRORQL]DV\RQX VDSWDQÃUNHQ N. meningitidis, N. lactamica ve N.polysacchareaWDÃºÃ\ÃFÃOÃÃ·ÃQDUDVWODQPDPÃÃºWÃUÃ¸]ROHHGLOHQNeisseriaVXÃºODUÃQÃQ1&&/6NULWHUOHULQHX\JXQRODUDNSHQLVLOLQ0Ã¸.GHÃ·HUOHUL VDSWDQPÃÃºWÃU 6XÃºODUÃQ Â¶VÃQÃQ SHQLVLOLQH GLUHQoOL Â¶XQXQ LVH RUWDNeisseria türünün N.GÂ]H\GH GLUHQoOL ROGXÃ·X VDSWDQPÃÃºWÃU (Q ID]OD GLUHQo J|VWHUHQelongata (%94), en fazla orta düzeyde direnç gösteren türün ise N. flavescens (%40)ROGXÃ·XJ|UÂOPÂÃºWÂUPenisiline dirençli olan Neisseria VXÃºODUÃQÃQ Â¶ÂQGH EHWD-laktamazN.cinereaÂ¶QÃQ HQ ID]OD EHWD-laktamaz üretenÂUHWLPL VDSWDQPÃÃºWÃU %XQODUGDQWÂUROGXÃ·XN.flavescens'in ise beta-ODNWDPD]ÂUHWPHGLÃ·LVDSWDQPÃÃºWÃUAnahtar kelimeler: Neisseria meningitidis, kommensal Neisseria türleri,kolonizasyon, penisilin direnci

Abstract:
N. meningitidis is a cause of endemic and epidemic disease in developed anddeveloping countries. The incidence of meningococcal disease appears topredominate in children under school age in the western world. Colonization of thenasopharynx is the initial step in the pathogenesis of disease due to N. meningitidis.The rate of carriage is related to several factors such as age, socioeconomic class,and the presence of actual disease in a community.Researchers suggests that commensal Neisseria species in nasopharynx aredeveloping antibodies against N. meningitidis infection.In recent years reported that in many countries penicillin susceptibility of N.meningitidis is decrease. Researchers suggest that decreased susceptibility ofpenicillin in N. meningitidis caused by the uptake of genetic material from thenaturally penicillin resistant, commensal species of Neisseria. For this reasoncommensal Neisseria species in the nasopharynx may identifying and the rates ofpenicillin susceptibility should have determined.Faculty and 284 Neisseria species were isolated. Determined that, the mostcolonization species of Neisseria is Neisseria subflava biovar perflava (%33), the lessone is N. cinerea (%1). And we didn?t isolated N. meningitidis, N. lactamica and N.polysaccharea.The MIC of penicillin of the isolates were determined by agar dilution methodaccordance with the NCCLS criteria. We determined that the 76% of strains areresistant and 9% of them are mediated resistant to penicillin. The most resistantNeisseria species is N. elongata (%94) and the most mediated resistant species is N.flavescens (%40).Of the 15% Neisseria isolates, which are resistant to penicillin, producing beta-lactamase. The highest beta-lactamase producing rate was seen in N.cinerea (%50)strains, however N.flavescens strains was not produced beta-lactamase.Key words: Neisseria meningitidis, commensal Neisseria species, colonization,penicillin resistance