Yeni geliştirilmiş bir takviye edici ürünün anti-influenza virüs aktivitesinin ın vitro ve ın vivo araştırılması
In vitro and in vivo investigation of anti-influenza virus activity of a newly developed supplementary product
Yazar: NESLİHAN AYDEMİR
Danışman: PROF. DR. CELAL KURTULUŞ BURUK
Karadeniz Teknik Üniversitesi / Sağlık Bilimleri Enstitüsü / Tıbbi Mikrobiyoloji Ana Bilim Dalı
Derece: Doktora, 2024
Konu: Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji = Infectious Diseases and Clinical Microbiology

Özet:
Some herbal products are designed to support health and are commercialized as
supplements and put into circulation on the market However, the biological properties of
most of these products have not been established by applying scientific rules and analyzing
them in a standardized manner. Various methods can be used to determine the biological
effects and mechanisms of action of herbal products. Although some methods are more
standardized, the standardization of methods, especially in the studies of antiviral activity of
essential oils against viruses and in determining their cytotoxicity, is insufficient. In this
study, it was aimed to determine the appropriate methodological approach for the evaluation
of the efficacy and toxicity of the herbal supplement "Coldmix® İnhaler Damla" against
influenza virus.
In this study, the in vitro cytotoxicity of "Coldmix® İnhaler Damla", a blend of some
compounds derived from eucalyptus and spruce plants, was evaluated using MDCK cells.
The effect of the product on anti-influenza virus (A/PR/8/34 strain) was investigated in vitro
using MDCK cells and in vivo using an animal model using BALB/c mice.
It was determined that the vapor phase of ptoduct did not show toxic effects on
MDCK cells at the recommended concentrations. It was observed that infected mice exposed
to product started the recovery process (weight gains) earlier than infected mice that were
not subjected to any treatment. In addition, when the lung homogenates of the mice whose
lungs were randomly selected and removed on the 6th day of the experimental design were
examined, it was calculated that those exposed to product had less virus shedding than the
control group. Histopathologically, it was observed that the lung tissue damage of the mice
in the product group was less than in the positive control group. In conclusion, the shortterm biological effects of the commercially available "Coldmix® Inhaler Drops" against the
influenza virus were examined with scientific methods and it was evaluated that it could
contribute to the recovery process from the disease without any toxic effects. However, in
order to make a definitive statement in favor of or against the commercial product, it would
be more useful to conduct such studies with larger experimental groups and long-term
planning in order to reveal the biological effects of the product and to monitor its side effects.
Keywords: Antiviral activity, Cytotoxicity, Dietary supplement, Influenza virus

Abstract:
Some herbal products are designed to support health and are commercialized as
supplements and put into circulation on the market However, the biological properties of
most of these products have not been established by applying scientific rules and analyzing
them in a standardized manner. Various methods can be used to determine the biological
effects and mechanisms of action of herbal products. Although some methods are more
standardized, the standardization of methods, especially in the studies of antiviral activity of
essential oils against viruses and in determining their cytotoxicity, is insufficient. In this
study, it was aimed to determine the appropriate methodological approach for the evaluation
of the efficacy and toxicity of the herbal supplement "Coldmix® İnhaler Damla" against
influenza virus.
In this study, the in vitro cytotoxicity of "Coldmix® İnhaler Damla", a blend of some
compounds derived from eucalyptus and spruce plants, was evaluated using MDCK cells.
The effect of the product on anti-influenza virus (A/PR/8/34 strain) was investigated in vitro
using MDCK cells and in vivo using an animal model using BALB/c mice.
It was determined that the vapor phase of ptoduct did not show toxic effects on
MDCK cells at the recommended concentrations. It was observed that infected mice exposed
to product started the recovery process (weight gains) earlier than infected mice that were
not subjected to any treatment. In addition, when the lung homogenates of the mice whose
lungs were randomly selected and removed on the 6th day of the experimental design were
examined, it was calculated that those exposed to product had less virus shedding than the
control group. Histopathologically, it was observed that the lung tissue damage of the mice
in the product group was less than in the positive control group. In conclusion, the shortterm biological effects of the commercially available "Coldmix® Inhaler Drops" against the
influenza virus were examined with scientific methods and it was evaluated that it could
contribute to the recovery process from the disease without any toxic effects. However, in
order to make a definitive statement in favor of or against the commercial product, it would
be more useful to conduct such studies with larger experimental groups and long-term
planning in order to reveal the biological effects of the product and to monitor its side effects.
Keywords: Antiviral activity, Cytotoxicity, Dietary supplement, Influenza virus