Streptozotosin ile diyabet oluşturulmuş ratlarda stobadinin karaciğer dokusu protein oksidasyonuna etkisinin araştırılması
Effect of pridoindole stobadine on liver tissue protein oxidation of STZ-Diabetic rats
Yazar: AHMET CUMAOĞLU
Danışman: PROF.DR. CEMAL ÇEVİK
Gazi Üniversitesi / Sağlık Bilimleri Enstitüsü / Tıbbi Biyokimya Ana Bilim Dalı
Derece: Yüksek Lisans, 2007
Konu: Biyokimya = Biochemistry
Dizin: Diabetes mellitus = Diabetes mellitus ; Oksidatif stres = Oxidative stress

Özet:
Serbest radikalerle gercekle§en oksidatif protein hasan diyabet gibi gibipatolojik olaylann gelisjminde onemli bir rol oynar. Proteinler oksijen ve nitrojenradikaleri ie hasara ugrayabilir bu da proteinlerde enzimatik aktivite kaybi ve aminoasiterin karbonil kahntanna donu§mesine neden olabilir. Aynca serbest radikalerinproteinlerin -SH (Tiyol) gruplannin oksidasyonuna neden oldugu bilinmektedir.Proteinlehn redoks potansiyelerinin varligi temel hucre onksiyaonlannm devami icinonemlidir. Dolayisiyla proteinlerdeki yapisal degisjmere bagh geli§en yeni molekuermekanizmalar diyabetik komplikasyonlann gelismesine liderlik etmektedir. Bucahsmada streptozotozin ie diyabet olusturulan ve kontrol ratarda pridoindolstobadin (STB; 24,7 mg/kg/gun) edavisinin karaciger dokusunda proteinoksidasyonu belirtecleri zerine etkisini ara§trdk.Qah§mamzda ratar pehton igi streptozotosin (55/mg/kg) enjeksiyonuie diyabet yapidi ve rastgele secien denekler, kontrol (n=7), kontrol+stobadin (n=7),diyabet (n=7), diyabet+stobadin (n=7) olmak zere dort gruba aynd. Karacigerhomojenatannda, nitrotirozin seviyeleri ELiSA kiti ie, ieri oksidasyon protein runleri(AOPP) ve tiyol dzeyleri spektrofotometrik yontemerle, protein karbonil seviyeleriise kolorimetrik deney kiti ie olcud. Tiyol seviyelerinin diyabetik ratarda dstugu,ancak AOPP ve karbonil dzeylerinin ise kontrole kiyasla arttgi (p<0,05) gozlemendi.Bununla birlikte diyabetik ratarda stobadin edavisi, tiyol dzeylerinde art§a, AOPPve protein karbonil bakiyelerinde istatistiksel olarak anlamh derecede azah§a (p<0,05)neden olmustur. Ancak 3-nitrotirozin seviyelerinde turn gruplarda anlamh degisimbelirlenmedi.Wistar-Abino ratann doku protein oksidasyon belirtegleri bakimmdandort grup arasmda ki istatistiksel karsiastrma Mann-Whitney U esti kulaniarakyapidi ve p<0,05 istatistiksel olarak anlamh kabul edidi.Bu cahsmanm sonuclanna gore, diyabetin geli§imine neden olabiecekoksidatif protein hasannm onlenmesine, stobadin tedavisinin oksidatif stresi azalarakkatkida bulunabiecegi vurgulanmaktadr.

Abstract:
The oxidatve protein damage provoked by reactve ree radicals hasbeen demonstrated o play a signicant role n several pathological events such asdiabetes. Proteins can also be damaged by oxygen and nirogen radicals, eading oa oss of enzymatc actviy and the conversion of amno acids to carbonyl derivatvesand t has already been shown hat ree radicals cause oxidaton of protein - Hgroups. Considerable evidence ndicates hat he maintenance of protein redoxstatus s of fundamental mportance or cell functon, herefore structural changes nproteins are considered o be among he molecular mechanisms eading o diabetccomplcatons, in he present study, we determned he efect of pridoindolestobadine supplementaton (STB; 24.7 mg/kg/day) on he markers of oxidatveprotein damage n he ver ssue of TZ- diabetc rats and heir control and reatedgroups.Dabetes was nduced by ntraperitonal njecton of streptozotosin (55mg/kg) and rats were randomy divided nto our groups as olows: control (K; n = 7),controlSTB (KTB; n=7), Dabet (DiA; n=7) and DabetSTB (DTB; n=7). 3-Nrotyrosine evels were measured by ELiSA. Thiols and AOPP evels of verhomogenates were studied by spectrophotometric methodand Protein carbonyl levelswere determned by colorimetric assay kit. Total, protein and non-protein hiol levelswere signicanty decreased n diabetc rats. On he other hand protein carbonylcontent and advanced oxidaton protein product levels were signicanty ncreased ndiabetc group compared w h hose of he controls (p<0,05). Stobadine reatmented o signicanty ncrease hiol parameters and decreased protein carbonyl contentand advanced oxidaton protein product (AOPP) evels n diabetSTB group (p<0.05).3-nirotyrosine evels weren't dierent in all groups.Tssue protein oxidaton parameters n he our groups of Wstar-Abinorats were compared using he Mann-Whiney U-test. When not specied p<0.05 wasconsidered signicant.The resuls of his study suggest hat stobadine decreased oxidatvestres and may be efectve n preventng oxidatve protein damage, which maycontribute to the development of diabetc complcatons.