protocolSection identificationModule nctId: NCT06307509, orgStudyIdInfo id: GN23AN097, briefTitle: Adiposity and Immunometabolism in Sepsis, acronym: AIMS, statusModule overallStatus: NOT_YET_RECRUITING, startDateStruct date: 2024-03, primaryCompletionDateStruct date: 2025-04-30, completionDateStruct date: 2025-07-31, studyFirstPostDateStruct date: 2024-03-13, lastUpdatePostDateStruct date: 2024-03-13, sponsorCollaboratorsModule leadSponsor name: NHS Greater Glasgow and Clyde, class: OTHER, collaborators name: University of Glasgow, descriptionModule briefSummary: Obesity has been shown to increase adverse outcomes in some critically ill patients e.g. those with COVID-19. For patients with sepsis this association is less clear cut but there is evidence that body fat distribution, resulting from impaired subcutaneous adipose tissue function, is associated with adverse clinical outcomes in critical care. The investigators aim to study subcutaneous adipose tissue function in lean and obese sepsis patients in critical care and compare that to healthy controls.First, the study will investigate differences in adipose tissue function (inflammation and mitochondrial function) related to obesity. Second, the investigators will examine whether lean critically ill patients with sepsis have enhanced adipose tissue inflammation and mitochondrial dysfunction compared to lean controls and whether this is further exacerbated by obesity.Patients will be either undergoing emergency abdominal surgery, or will have been admitted to a critical care unit with a diagnosis of sepsis.The investigators will collect blood and adipose tissue biopsies from the patients, and these will be analysed for markers of inflammation and of mitochondrial function.The aim is to better understand the relationship between obesity, inflammation, mitochondrial dysfunction and sepsis. The investigators hope that this may improve the understanding of the pathophysiology of sepsis and allow more targeted interventions for patients based on differences in their baseline metabolic state., conditionsModule conditions: Obesity, conditions: Sepsis, designModule studyType: OBSERVATIONAL, designInfo observationalModel: CASE_ONLY, timePerspective: PROSPECTIVE, enrollmentInfo count: 60, type: ESTIMATED, armsInterventionsModule interventions name: Diagnosis of sepsis, outcomesModule primaryOutcomes measure: Difference in adipocyte cytokine secretion between patients with sepsis, and patients without sepsis, and between SAT and VAT samples, secondaryOutcomes measure: Differences in adipocyte size, secondaryOutcomes measure: Differences in mitochondrial morphology, secondaryOutcomes measure: Differences in adipocyte pro-inflammatory gene expression, secondaryOutcomes measure: Differences in adipocyte mitochondrial number, secondaryOutcomes measure: Differences in adipocyte mitochondrial viability, secondaryOutcomes measure: Differences in SVF cytokine secretion, secondaryOutcomes measure: Differences in capacity of pre-adipocytes to differentiate into mature adipocytes, eligibilityModule sex: ALL, minimumAge: 18 Years, stdAges: ADULT, stdAges: OLDER_ADULT, contactsLocationsModule locations facility: Department of Critical Care Medicine, Queen Elizabeth University Hospital, city: Glasgow, zip: G51 4TF, country: United Kingdom, geoPoint lat: 55.86515, lon: -4.25763, hasResults: False