protocolSection identificationModule nctId: NCT06277713, orgStudyIdInfo id: CME2023/069, briefTitle: Preventing Exercise Resistance With Sedentary Interruptions, acronym: PERSI, statusModule overallStatus: RECRUITING, startDateStruct date: 2024-02-19, primaryCompletionDateStruct date: 2024-10-30, completionDateStruct date: 2025-07-01, studyFirstPostDateStruct date: 2024-02-26, lastUpdatePostDateStruct date: 2024-03-04, sponsorCollaboratorsModule leadSponsor name: Hasselt University, class: OTHER, descriptionModule briefSummary: The goal of this randomised cross-over trial is to learn about the interaction between sedentary behaviour throughout the day and the metabolic effect of an exercise bout on that same day in office workers with an increased risk for chronic disease.The main question this study aims to answer is if the lipid-lowering effects of an exercise bout can be more pronounced by implementing alternations between a seated and a standing working position throughout the day.Participants will be asked to:* Complete three intervention periods for a duration of 2 days at their workplace,* Attend a supervised training session (60min) at the research facility at the end of each intervention period,* Attend three assessment days at the research facility where postprandial metabolism will be evaluated after a standardised meal test., conditionsModule conditions: Sedentary Behavior, conditions: Lipid Metabolism Disorder, conditions: Exercise, designModule studyType: INTERVENTIONAL, phases: NA, designInfo allocation: RANDOMIZED, interventionModel: CROSSOVER, interventionModelDescription: Participants will follow three regimes of 2 days each including a sedentary control, a sedentary with exercise and a standing breaks with exercise regimen. The sequence of the regimens will be randomised. Each regimen will be followed by a minimum wash-out period of 7 days during which subjects will continue their normal lifestyle.We will use a balanced design with 6 randomisation blocks (ABC, ACB, BAC,BCA, CAB, CBA). The randomisation will be computer-generated and allocation will be determined by a member of the research team drawing a sealed, non-translucent envelop with herein written the scheme., primaryPurpose: PREVENTION, maskingInfo masking: SINGLE, maskingDescription: The outcome assessor will be blinded to the allocation of participants as samples will be code labeled., whoMasked: OUTCOMES_ASSESSOR, enrollmentInfo count: 24, type: ESTIMATED, armsInterventionsModule interventions name: Standing interruptions, interventions name: Exercise bout, interventions name: Sedentary, outcomesModule primaryOutcomes measure: Total and incremental area under the curve of postprandial response in lipid metabolism, secondaryOutcomes measure: Total and incremental area under the curve of glucose and insulin postprandial responses, secondaryOutcomes measure: Resting metabolic rate, eligibilityModule sex: ALL, minimumAge: 30 Years, maximumAge: 65 Years, stdAges: ADULT, stdAges: OLDER_ADULT, contactsLocationsModule locations facility: Hasselt University, status: RECRUITING, city: Diepenbeek, state: Limburg, zip: 3590, country: Belgium, contacts name: Bert Op 't Eijnde, Prof. dr., role: CONTACT, phone: +3211292121, email: bert.opteijnde@uhasselt.be, contacts name: Jen Vanherle, Drs., role: CONTACT, email: jen.vanherle@uhasselt.be, contacts name: Bert Op 't Eijnde, Prof. dr., role: PRINCIPAL_INVESTIGATOR, contacts name: Jen Vanherle, Drs., role: SUB_INVESTIGATOR, geoPoint lat: 50.90769, lon: 5.41875, hasResults: False