protocolSection identificationModule nctId: NCT06382298, orgStudyIdInfo id: PROJECT00009332, briefTitle: Cottonseed Oil Versus Matched PUFA Effects, statusModule overallStatus: NOT_YET_RECRUITING, startDateStruct date: 2024-08, primaryCompletionDateStruct date: 2026-12, completionDateStruct date: 2028-05, studyFirstPostDateStruct date: 2024-04-24, lastUpdatePostDateStruct date: 2024-04-26, sponsorCollaboratorsModule leadSponsor name: University of Georgia, class: OTHER, collaborators name: United States Department of Agriculture (USDA), descriptionModule briefSummary: Cardiovascular disease risk factors, including higher BMIs and poor cholesterol profiles, are on the rise and contribute to the United States' growing disease burden. Cottonseed oil (CSO) is found readily in the food supply, and the investigator's previous studies have demonstrated that incorporating CSO into the diet is sufficient to improve fasting cholesterol profile and improve postprandial lipid and/or glycemic responses in both healthy, and at-risk populations. This study aims to compare CSO to a fatty acid composition-matched diet, on changes in fasting and postprandial lipid metabolism and markers of chronic disease risk.The specific aims are:* Examine the impact of CSO vs. PUFA on fasting and postprandial lipids.* Examine the impact of CSO on other markers of chronic disease risk.Participants will be asked to:* Consume provided meal replacement shakes daily for 28-days.* Attend three weekly short visits for fasting blood draws, body measurements, and collect the next week of study materials,* Attend two longer (5.5h) testing visits which include eating a standardized breakfast meal and having blood drawn periodically before and after breakfast.Researchers will compare CSO vs. PUFA and control groups (receiving a mixture of oils) to see if CSO is unique in imparting health benefits when compared with similar matched oil diets., conditionsModule conditions: Dyslipidemia, conditions: Overweight and Obesity, conditions: Nutrition, Healthy, designModule studyType: INTERVENTIONAL, phases: NA, designInfo allocation: RANDOMIZED, interventionModel: PARALLEL, interventionModelDescription: Three groups of 28-day parallel feeding trials with two groups receiving 20% of energy needs from CSO, or matched PUFA diet, and one group serving as control receiving 20% of energy needs as a mixture of oils that match the fatty acid profile of the average American intake., primaryPurpose: PREVENTION, maskingInfo masking: DOUBLE, maskingDescription: Participants are blinded to oil intervention group. Investigator blinded to participant's oil intervention group., whoMasked: PARTICIPANT, whoMasked: INVESTIGATOR, enrollmentInfo count: 90, type: ESTIMATED, armsInterventionsModule interventions name: COTTONSEED OIL, interventions name: MATCHED PUFA DIET, interventions name: CONTROL, outcomesModule primaryOutcomes measure: Change in fasting serum lipoprotein and cholesterol concentrations, primaryOutcomes measure: Change in fasting serum lipoprotein particle numbers, primaryOutcomes measure: Change in fasting and postprandial plasma triglyceride concentrations, primaryOutcomes measure: Change in fasting and postprandial plasma non-esterified fatty acid (NEFA) concentrations, primaryOutcomes measure: Change in fasting and postprandial plasma glucose concentrations, primaryOutcomes measure: Change in fasting and postprandial plasma insulin concentrations, primaryOutcomes measure: Change in fasting and postprandial plasma appetite control hormones concentrations, primaryOutcomes measure: Change in fasting and postprandial subjective feelings related to appetite, primaryOutcomes measure: Change in fasting and postprandial plasma Malondialdehyde (MDA), primaryOutcomes measure: Change in fasting and postprandial plasma total antioxidant capacity, primaryOutcomes measure: Change in fasting inflammatory cytokine concentrations, primaryOutcomes measure: Change in fasting plasma markers of coagulation potential, primaryOutcomes measure: Change in fasting and postprandial plasma angiopoietin-like (ANGPTL) proteins, primaryOutcomes measure: Change in fasting insulin resistance metrics, primaryOutcomes measure: Desaturation index, secondaryOutcomes measure: Change in fasting serum hepatic enzymes, secondaryOutcomes measure: Change in fasting serum hepatic proteins, secondaryOutcomes measure: Change in fasting serum bilirubin, secondaryOutcomes measure: Change in additional fasting plasma inflammatory cytokine concentrations, secondaryOutcomes measure: Change in additional fasting plasma markers of coagulation potential, secondaryOutcomes measure: Change in acute dietary intake, otherOutcomes measure: Change in blood pressure, otherOutcomes measure: Change in body weight, otherOutcomes measure: Change in body composition, otherOutcomes measure: Change in diet composition, otherOutcomes measure: Change in fasting tocopherol concentrations, otherOutcomes measure: Change in anthropometric circumferences, otherOutcomes measure: Change in resting metabolic rate, otherOutcomes measure: Change in Perceived Stress, otherOutcomes measure: Change in anxiety, otherOutcomes measure: Change in self reported physical activity levels, otherOutcomes measure: Change in Body Mass Index (BMI), eligibilityModule sex: ALL, minimumAge: 25 Years, maximumAge: 75 Years, stdAges: ADULT, stdAges: OLDER_ADULT, contactsLocationsModule locations facility: University of Georgia, city: Athens, state: Georgia, zip: 30602, country: United States, geoPoint lat: 33.96095, lon: -83.37794, hasResults: False