protocolSection identificationModule nctId: NCT06302933, orgStudyIdInfo id: ANRS 12414 PediacamNEG, briefTitle: Negative Serology by Immunoenzymatic Test (EIA) in HIV-infected Children Treated Early With Antiretroviral in the ANRS-Pediacam Study: Pathophysiological Mechanisms, acronym: PediacamNEG, statusModule overallStatus: NOT_YET_RECRUITING, startDateStruct date: 2024-03-01, primaryCompletionDateStruct date: 2025-08-30, completionDateStruct date: 2025-08-30, studyFirstPostDateStruct date: 2024-03-12, lastUpdatePostDateStruct date: 2024-03-12, sponsorCollaboratorsModule leadSponsor name: ANRS, Emerging Infectious Diseases, class: OTHER_GOV, collaborators name: Centre Pasteur du Cameroun, collaborators name: Centre Mère et Enfant de la Fondation Chantal Biya, collaborators name: Centre Hospitalier D'essos, collaborators name: Hospital General De Douala, collaborators name: Centre Hospitalier Universitaire d'Orléans, collaborators name: Institut Pasteur, collaborators name: Hopital Universitaire Robert-Debre, collaborators name: Université Paris-Sud, descriptionModule briefSummary: The objective of the study is to identify the pathophysiological mechanisms responsible for the induction and maintenance of negative serologies by EIA tests in HIV-infected children treated early with HAART in the ANRS 12225-Pediacam III cohort in CameroonThe hypothesis of better control of HIV infection through interactions between immunological, viral, and genetic factors was made to build the following objectives:* Immunological aspect: lack of humoral response or immune activation* Virological aspect: Reduced HIV reservoir size* Determine the HLA phenotype in the different groups of children included and the KIR genotypes., conditionsModule conditions: HIV Infections, designModule studyType: INTERVENTIONAL, phases: NA, designInfo allocation: NA, interventionModel: SINGLE_GROUP, interventionModelDescription: Study type: a study nested in the ANRS 12225 - Pediacam III cohort, comprising two phases* A retrospective phase: case-control study The analyzed data are those collected previously or measured from the already available bio bank, within the framework of the Pediacam III cohort during the primary infection phase before the initiation of HAART, at 6 months after the end of the first series of EPI vaccines, and at 2 years.* A prospective phase: cross-sectional study Based on an ad hoc bio bank created for parameters we couldn't measure on the existing bio bank, primaryPurpose: HEALTH_SERVICES_RESEARCH, maskingInfo masking: NONE, enrollmentInfo count: 451, type: ESTIMATED, armsInterventionsModule interventions name: Blood sampling, outcomesModule primaryOutcomes measure: Level of pro-inflammatory and anti-inflammatory cytokines, chimiokines in the plasma, primaryOutcomes measure: Level of pro-inflammatory and anti-inflammatory cytokines, chimiokines in the plasma, primaryOutcomes measure: Level of pro-inflammatory and anti-inflammatory cytokines, chimiokines in the plasma, primaryOutcomes measure: Level of pro-inflammatory and anti-inflammatory cytokines, chimiokines in the plasma, primaryOutcomes measure: Level of pro-inflammatory and anti-inflammatory cytokines, chimiokines in the plasma, primaryOutcomes measure: Level of pro-inflammatory and anti-inflammatory cytokines, chimiokines in the plasma, primaryOutcomes measure: Level of pro-inflammatory and anti-inflammatory cytokines, chimiokines in the plasma, secondaryOutcomes measure: - Humoral response to vaccines against tetanus, pertussis, and viral hepatitis B, secondaryOutcomes measure: - Functional and phenotypic characterization of B and T lymphocytes, secondaryOutcomes measure: - Size of the HIV reservoir, secondaryOutcomes measure: - Residual viremia in perinatally HIV-infected adolescent, secondaryOutcomes measure: - Level of HIV plasma p24, secondaryOutcomes measure: - HLA phenotype and the KIR genotypes, eligibilityModule sex: ALL, stdAges: CHILD, stdAges: ADULT, stdAges: OLDER_ADULT, contactsLocationsModule locations facility: Centre Pasteur Cameroun, city: Yaounde, zip: 1274, country: Cameroon, contacts name: Mathurin C Tejiokem, role: CONTACT, phone: +237222231803, email: tejiokem@pasteur-yaounde.org, geoPoint lat: 3.86667, lon: 11.51667, hasResults: False