nctId: NCT06349226 id: DISCOLAB briefTitle: Identification of Biomarkers and Molecular Targets Involved on Intervertebral Disc Degeneration and Discogenic Pain overallStatus: RECRUITING date: 2018-05-22 date: 2025-05-21 date: 2025-05-21 date: 2024-04-05 date: 2024-04-05 name: Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico class: OTHER briefSummary: Low back pain, associated with intrinsic disorders of the spine, is a very frequent clinical condition that is accompanied by high morbidity with effects both on psychosocial aspects, and health care system. It occurs in approximately 80% of the population throughout their lives. Most low back pain is associated with intervertebral disc degeneration (IDD) associated with neuroinflammation and pain. In this context, the study of sphingolipid metabolism can play an important role in the identification of new molecules responsible for the degenerative process. Sphingolipids, in fact, are a class of molecules that are implicated in multiple signal pathways, such as proliferation, degradation of the extracellular matrix, inflammatory state, apoptosis and migration. In particular, sphingosine-1-phosphate (S1P), an intermediate of sphingolipid metabolism, acts as a pro-inflammatory mediator, predominantly in the extracellular environment, regulating important cellular properties related to inflammatory potential and pain. The objective of this study is to characterize the degenerative process in cells isolated from degenerated human intervertebral discs from both at cellular and molecular levels in order to identify new targets implicated in degenerative processes, including sphingolipid signaling pathway. conditions: Intervertebral Disc Degeneration conditions: Discogenic Pain conditions: Discogenic Disease studyType: OBSERVATIONAL observationalModel: OTHER timePerspective: PROSPECTIVE count: 160 type: ESTIMATED name: cell isolation from intervertebral disc tissue and biomarker investigation measure: Characterization of degenerated intervertebral disc cells measure: Development of cellular model platform sex: ALL stdAges: CHILD stdAges: ADULT stdAges: OLDER_ADULT facility: Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico status: RECRUITING city: Milan zip: 20122 country: Italy name: Giovanni Marfia, MD, Phd role: CONTACT phone: 0255035502 phoneExt: +39 email: giovanni.marfia@policlinico.mi.it name: Stefania E Navone, PhD role: CONTACT phone: 0255034268 phoneExt: +39 email: stefania.navone@policlinico.mi.it name: Mauro Pluderi, MD role: PRINCIPAL_INVESTIGATOR name: Giovanni Marfia, MD, PhD role: SUB_INVESTIGATOR name: Stefania E Navone, PhD role: SUB_INVESTIGATOR name: Laura Guarnaccia, PhD role: SUB_INVESTIGATOR name: Laura Begani, MSc role: SUB_INVESTIGATOR name: Marco Locatelli, MD, PhD role: SUB_INVESTIGATOR name: Stefano Borsa, MD role: SUB_INVESTIGATOR name: Giulio Bertani, MD role: SUB_INVESTIGATOR name: Claudia Fanizzi, MD role: SUB_INVESTIGATOR name: Giorgio Fiore, MD role: SUB_INVESTIGATOR name: Luigi Schisano, MD role: SUB_INVESTIGATOR name: Manuela Caroli, MD role: SUB_INVESTIGATOR name: Antonella Ampollini, MD role: SUB_INVESTIGATOR name: Elena Pirola, MD role: SUB_INVESTIGATOR lat: 45.46427 lon: 9.18951 hasResults: False