nctId: NCT06367985 id: 03/24/DD-BVMD briefTitle: CAPA-IVM Culture With Low Oxygen Tension overallStatus: NOT_YET_RECRUITING date: 2024-05-03 date: 2024-06-01 date: 2025-06-01 date: 2024-04-16 date: 2024-04-29 name: Mỹ Đức Hospital class: OTHER name: Vrije Universiteit Brussel briefSummary: Capacitation in-vitro maturation (CAPA-IVM) has recently been advanced in culturing oocytes from the germinal vesicle (GV) stage following mild or no controlled ovarian stimulation. Recent research suggested that O2 concentration may significantly regulate oocyte maturation and early embryo development through hypoxia-inducible factor (HIF). Nonetheless, it has been challenging to create the environmental culture conditions for addressing the optimal number of oocytes and the highest possibility of embryo development since consensus on the oxygen (O2) concentration index in the IVM culture environment has not been reached. Based on the outcomes of atmospheric O2 concentration (20%) and low O2 concentration (5%) during CAPA-IVM culture in mice, it has been hypothesized that a 5% O2 was the optimal culture condition for the pre-IVM step. A 20% O2 was more suitable for the IVM culture step. Therefore, this study is designed to enhance the CAPA-IVM culture system, improving treatment efficiency and providing various benefits for patients undergoing assisted reproductive technology. conditions: In Vitro Fertilization studyType: INTERVENTIONAL phases: NA allocation: NON_RANDOMIZED interventionModel: PARALLEL interventionModelDescription: Sibling oocytes primaryPurpose: TREATMENT masking: NONE count: 20 type: ESTIMATED name: Air Oxygen Concentration CAPA-IVM culture name: Low Oxygen Concentration CAPA-IVM culture measure: Maturation rate measure: Total number of oocytes retrieval measure: Number of patients with no oocyte retrieved measure: Number of MII oocytes measure: Number of GV oocytes measure: Number of patients with no matured oocyte measure: Number of 2PN oocytes measure: Fertilization rate measure: Abnormal fertilization rate measure: Number of patients with no day-3 embryo measure: Number of day-3 embryos measure: Number of good quality Day-3 embryos measure: Number of frozen day-3 embryos measure: Number of blastocyst (day 5 or day 6 embryo) measure: Number of patients with no blastocyst measure: Number of good quality blastocysts measure: Number of frozen blastocysts measure: Number of embryos transferred measure: Quality of embryos transferred (Grade 1, Grade 2, Grade 3) measure: Positive pregnancy test rate measure: Implantation rate measure: Clinical pregnancy rate measure: Ectopic pregnancy rate measure: Ongoing pregnancy rate measure: Miscarriage <12 weeks rate (Early miscarriage) measure: Miscarriage <22 weeks rate (late miscarriage) measure: Live birth rate measure: Multiple pregnancy rate measure: Multiple delivery rate measure: Mode of delivery measure: Gestational age at birth measure: Birth weight measure: Very low birth weight rate measure: Low birth weight rate measure: High birth weight rate measure: Very high birth weight rate measure: Small for gestational age rate measure: Large for gestational age rate measure: Hypertension in pregnancy rate measure: Gestational diabetes mellitus rate measure: Still birth rate measure: Premature birth rate measure: Antepartum haemorrhage rate measure: Major congenital abnormalities rate measure: Neonatal mortality rate measure: NICU admission rate measure: Reason for NICU admission sex: FEMALE minimumAge: 18 Years maximumAge: 42 Years stdAges: ADULT facility: My Duc Hospital city: Ho Chi Minh City country: Vietnam name: Tuong M Ho, MSc, MD role: CONTACT phone: +84 90 3633377 email: tuongho.ivfmd@gmail.com lat: 10.82302 lon: 106.62965 hasResults: False