ghanaopendata/ghanaian-research-english huggingface dataset:
Measuring concentrations of insulin-like growth factor-l with an enzyme-linked immunosorbent antibody assay in plasma samples from Holstein cows

This study describes the use of a commercial non extraction ELISA to quantify total concentrations of insulin-like growth factor-l (IGF-I) in plasma samples from Holstein cows fed pasture-based diets varying in dry matter and metabolizable energy density. The assays were conducted using the protocols provided by the manufacturer. The ELISA was verified for linearity, accuracy in measuring IGF-I from spiked plasma samples, and precision involving variation within and between assays. Validation also involved comparing results of the ELISA against an established RIA after defatted plasma samples were subjected to acid gel HPLC to dissociate and separate IGF and IGF binding protein complexes. Validation results had low coefficients of variation (CV; intraassay CV of less than 6% and interassay CV of less than 8%) and a high recovery percentage of IGF-I (79%) from samples spiked with unbound IGF-I. The coefficients of determination between the ELISA and the RIA reference assay were 0.90 in 2 separate assays. Associations between the RIA and the ELISA were higher, and the limits of agreement at 95% confidence intervals were narrower compared with those between the RIA and a modified ELISA procedure in which IGF binding protein complexes were extracted using HPLC. The samples were obtained from cows sampled over a period of 5 wk. These results demonstrated that the DSL-10-2800 nonextraction IGF-I ELISA was acceptably specific and sufficiently sensitive to be used to measure the extent and patterns of change in the plasma concentrations of IGF-I in samples from lactating Holstein cows

Source: UG
Measuring Coverage in MNCH: Validating Women's Self-Report of Emergency Cesarean Sections in Ghana and the Dominican Republic

Background: Cesarean section is the only surgery for which we have nearly global population-based data. However, few surveys provide additional data related to cesarean sections. Given weaknesses in many health information systems, health planners in developing countries will likely rely on nationally representative surveys for the foreseeable future. The objective is to validate self-reported data on the emergency status of cesarean sections among women delivering in teaching hospitals in the capitals of two contrasting countries: Accra, Ghana and Santo Domingo, Dominican Republic (DR). Methods and Findings: This study compares hospital-based data, considered the reference standard, against women's self-report for two definitions of emergency cesarean section based on the timing of the decision to operate and the timing of the cesarean section relative to onset of labor. Hospital data were abstracted from individual medical records, and hospital discharge interviews were conducted with women who had undergone cesarean section in two hospitals. The study assessed sensitivity, specificity, and positive predictive value of responses to questions regarding emergency versus non-emergency cesarean section and estimated the percent of emergency cesarean sections that would be obtained from a survey, given the observed prevalence, sensitivity, and specificity from this study. Hospital data were matched with exit interviews for 659 women delivered via cesarean section for Ghana and 1,531 for the Dominican Republic. In Ghana and the Dominican Republic, sensitivity and specificity for emergency cesarean section defined by decision time were 79% and 82%, and 50% and 80%, respectively. The validity of emergency cesarean defined by operation time showed less favorable results than decision time in Ghana and slightly more favorable results in the Dominican Republic. Conclusions: Questions used in this study to identify emergency cesarean section are promising but insufficient to promote for inclusion in international survey questionnaires. Additional studies which confirm the accuracy of key facility-based indicators in advance of data collection and which use a longer recall period are warranted. Â© 2013 TunÃ§alp et al.

Source: UG
Measuring Governance Institutions’ Success in Ghana: The Case of the Electoral Commission 1993-2008

The need for state institutions to promote good governance is now a necessary condition for consolidating new democracies. However, achieving this objective represents a daunting challenge for the emerging constitutional bodies in Ghana. This article sets out to examine the Electoral Commission's (EC) efforts at institutionalising good governance in the management of the electoral process. Against the backdrop of failed electoral process in most African countries, the EC has organised four successful general elections with marginal errors. The most distinguishing factors accounting for the EC's success were largely, but not exclusively, the making of the electoral process transparent, fostering agreement on the rules of the game and asserting its autonomy in relation to the performance of its mandate. What needs to be done is electoral reform to overcome challenges posed by delayed adjudication of post-election disputes and executive financial control of the EC. This will require the creation of an electoral court to deal swiftly and impartially with election disputes and a special electoral fund to insulate the EC from government's financial manipulations

Source: UG
Measuring Health Systems Strength and Its Impact: Experiences from the African Health Initiative

Background: Health systems are essential platforms for accessible, quality health services, and population health improvements. Global health initiatives have dramatically increased health resources; however, funding to strengthen health systems has not increased commensurately, partially due to concerns about health system complexity and evidence gaps demonstrating health outcome improvements. In 2009, the African Health Initiative of the Doris Duke Charitable Foundation began supporting Population Health Implementation and Training Partnership projects in five sub-Saharan African countries (Ghana, Mozambique, Rwanda, Tanzania, and Zambia) to catalyze significant advances in strengthening health systems. This manuscript reflects on the experience of establishing an evaluation framework to measure health systems strength, and associate measures with health outcomes, as part of this Initiative. Methods: Using the World Health Organization's health systems building block framework, the Partnerships present novel approaches to measure health systems building blocks and summarize data across and within building blocks to facilitate analytic procedures. Three Partnerships developed summary measures spanning the building blocks using principal component analysis (Ghana and Tanzania) or the balanced scorecard (Zambia). Other Partnerships developed summary measures to simplify multiple indicators within individual building blocks, including health information systems (Mozambique), and service delivery (Rwanda). At the end of the project intervention period, one to two key informants from each Partnership's leadership team were asked to list - in rank order - the importance of the six building blocks in relation to their intervention. Results: Though there were differences across Partnerships, service delivery and information systems were reported to be the most common focus of interventions, followed by health workforce and leadership and governance. Medical products, vaccines and technologies, and health financing, were the building blocks reported to be of lower focus. Conclusion: The African Health Initiative experience furthers the science of evaluation for health systems strengthening, highlighting areas for further methodological development - including the development of valid, feasible measures sensitive to interventions in multiple contexts (particularly in leadership and governance) and describing interactions across building blocks; in developing summary statistics to facilitate testing intervention effects on health systems and associations with health status; and designing appropriate analytic models for complex, multi-level open health systems.

Source: UG
Measuring Lineage: Implications for Family Violence Research in Sub-Saharan Africa

Previous research on family violence in sub-Saharan Africa highlighted the importance of lineage to women’s experiences of intimate partner violence (IPV). The findings suggest women in patrilineal societies face a greater risk of experiencing IPV than those in matrilineal societies. However, a major critique of this body of work is the operationalization of lineage with ethnicity. This study highlights the weaknesses/ strengths of using ethnicity as a proxy for lineage by comparing it to direct measures of lineage. Specifically, we tested the validity of lumping ethnic groups together to create lineage categories against measures that directly ask respondents to self-identify their lineage. We also explored the effects of lineage on different types of IPV. We used representative cross-sectional data collected between May and August 2022 from 1,624 ever-married Ghanaian women aged 18 years and older and residing in three major ecological zones—Coastal, Middle, and Northern Zones—that reflect differences in ecology, culture, and modernity in Ghana. Descriptive and multivariate statistical techniques were used to analyze the data. The findings suggest significant differences in direct (self-identified) and indirect (ethnic) measures of lineage. The majority of respondents who were classified as matrilineal or patrilineal based on their ethnic backgrounds self-reported as belonging to these lineage categories. Both direct and indirect measures of lineage were significantly associated with IPV. However, given the limited operationalization of lineage based on ethnicity, self-identified measures were more useful. While ethnicity remains an important proxy for lineage, self-identified measures of the construct are better if available.

Source: UG
Measuring naturally acquired ex vivo IFN-¿ responses to Plasmodium falciparum cell-traversal protein for ookinetes and sporozoites (CelTOS) in Ghanaian adults

Abstract Background A malaria vaccine that targets the sporozoite/liver stage parasites could potentially prevent blood stage infection and the associated clinical symptoms. Identification of sporozoite/liver stage antigens is, therefore, crucial for the development of effective vaccines. Cell-traversal protein for ookinetes and sporozoites (CelTOS) is a highly conserved antigen involved in sporozoite motility and hepatocyte invasion and has been shown to induce significant IFN-&#947; production in PBMCs from radiation-attenuated sporozoite-immunized malaria-na&#239;ve individuals. The aim of this study was to ascertain whether such CelTOS-specific recall responses are also induced in individuals with natural exposure to Plasmodium falciparum. Methods Ex vivo IFN-&#947; responses to 15mer overlapping peptide pools covering the entire sequence of CelTOS and five other candidate antigens, CSP, AMA1, MSP1, TRAP and LSA1, were characterized using PBMCs from 35 malaria exposed adults. Responses to four CelTOS peptide pools (CelTp1, CelTp2, CelTp3 and CelTp4), a pool containing peptides from the entire CelTOS antigen (CelTTp), and pools comprised of overlapping peptides from each of the other five malaria antigens were assessed by ex vivo ELISpot assay. A positive IFN-&#947; response for stimulants was defined by two criteria; a stimulation index of two or greater relative to the unstimulated control, and a difference of 10 or greater in spot forming cells between stimulant and the unstimulated control. Results Of the 35 volunteers tested, five had positive IFN-&#947; recall responses against the four different CelTOS pools while four volunteers made responses against the CelTTp pool; six volunteers were, therefore, positive with CelTOS. By contrast, six volunteers responded to AMA1, seven to LSA1, 15 to MSP1 and two volunteers responded against CSP and TRAP. Conclusions These results suggest natural malaria transmission induces CelTOS-specific ex vivo IFN-&#947; in Ghanaian adults and that the frequency of these responses was similar to those of other previously characterized malaria antigens. These findings support the further evaluation of CelTOS as a pre-erythrocytic candidate antigen for inclusion in a potential multi-antigen vaccine.

Source: UG
Measuring naturally acquired ex vivo IFN-γ responses to Plasmodium falciparum cell-traversal protein for ookinetes and sporozoites (CelTOS) in Ghanaian adults

The Department of Immunology conducts research in the field of immunology of infectious and non-infectious diseases. The Department has the overall goal of contributing knowledge to better diagnosis, management, control and prevention of infectious and non-infectious diseases in Ghana and worldwide. This is consistent with the overall strategy of the Noguchi Memorial Institute for Medical Research (NMIMR) and in line with the strategy of the College of Health Sciences of the University of Ghana.

Source: UG
Measuring naturally acquired immune responses to candidate malaria vaccine antigens in Ghanaian adults

Abstract Background To prepare field sites for malaria vaccine trials, it is important to determine baseline antibody and T cell responses to candidate malaria vaccine antigens. Assessing T cell responses is especially challenging, given genetic restriction, low responses observed in endemic areas, their variability over time, potential suppression by parasitaemia and the intrinsic variability of the assays. Methods In Part A of this study, antibody titres were measured in adults from urban and rural communities in Ghana to recombinant Plasmodium falciparum CSP, SSP2/TRAP, LSA1, EXP1, MSP1, MSP3 and EBA175 by ELISA, and to sporozoites and infected erythrocytes by IFA. Positive ELISA responses were determined using two methods. T cell responses to defined CD8 or CD4 T cell epitopes from CSP, SSP2/TRAP, LSA1 and EXP1 were measured by ex vivo IFN-&#947; ELISpot assays using HLA-matched Class I- and DR-restricted synthetic peptides. In Part B, the reproducibility of the ELISpot assay to CSP and AMA1 was measured by repeating assays of individual samples using peptide pools and low, medium or high stringency criteria for defining positive responses, and by comparing samples collected two weeks apart. Results In Part A, positive antibody responses varied widely from 17%-100%, according to the antigen and statistical method, with blood stage antigens showing more frequent and higher magnitude responses. ELISA titres were higher in rural subjects, while IFA titres and the frequencies and magnitudes of ex vivo ELISpot activities were similar in both communities. DR-restricted peptides showed stronger responses than Class I-restricted peptides. In Part B, the most stringent statistical criteria gave the fewest, and the least stringent the most positive responses, with reproducibility slightly higher using the least stringent method when assays were repeated. Results varied significantly between the two-week time-points for many participants. Conclusions All participants were positive for at least one malaria protein by ELISA, with results dependent on the criteria for positivity. Likewise, ELISpot responses varied among participants, but were relatively reproducible by the three methods tested, especially the least stringent, when assays were repeated. However, results often differed between samples taken two weeks apart, indicating significant biological variability over short intervals.

Source: UG
Measuring naturally acquired immune responses to candidate malaria vaccine antigens in Ghanaian adults.

The Department of Immunology conducts research in the field of immunology of infectious and non-infectious diseases. The Department has the overall goal of contributing knowledge to better diagnosis, management, control and prevention of infectious and non-infectious diseases in Ghana and worldwide. This is consistent with the overall strategy of the Noguchi Memorial Institute for Medical Research (NMIMR) and in line with the strategy of the College of Health Sciences of the University of Ghana.

Source: UG
Measuring Positive Mental Health and Depression in Africa: A Variable-Based and Person-Centred Analysis of the Dual-Continua Model

The dual-continua model of mental health provides a contemporary framework for conceptualising and operationalizing mental health. According to this model, mental health is distinct from but related to mental illness, and not the opposite or merely the absence of psychopathology symptoms. To examine the validity of the dual-continua model, previous studies have either applied variable-based analysis or confirmatory factor analysis (CFA), or used predetermined cut-off points for subgroup division. The present study extends this contribution by subjecting data from an African sample to both CFA and latent class analysis (LCA) to test the dual-continua model in Africa. We applied CFA separately for the Mental Health Continuum—Short Form (MHC-SF) and Patient Health Questionnaire—9 (PHQ-9); and LCA on combined item responses. College students (N = 892; average age = 22.74, SD = 4.92; female = 58%) from Ghana (n = 309), Kenya (n = 262), and Mozambique (n = 232), and South Africa (n = 89) completed the MHC-SF and PHQ-9. With minor modifications to the measurement models, the CFA results of this study confirm the three-factor structure of the MHC-SF and a unidimensional solution for the PHQ-9. LCA results show the presence of three distinct latent classes: languishing with moderate endorsement of depressive symptoms (25.9%), flourishing with low endorsement of depressive symptoms (63.7%), and moderate mental health with high endorsement of depressive symptoms (10.4%). These findings further contribute to affirming the evidence for the dual- continuum model of mental health, with implications for the assessment of mental health, to inform policy, practice, and future research in community and clinical settings in Africa.

Source: UG
Measuring progress from 1990 to 2017 and projecting attainment to 2030 of the health-related Sustainable Development Goals for 195 countries and territories: a systematic analysis for the Global Burden of Disease Study 2017

Background Efforts to establish the 2015 baseline and monitor early implementation of the UN Sustainable Development Goals (SDGs) highlight both great potential for and threats to improving health by 2030. To fully deliver on the SDG aim of "leaving no one behind", it is increasingly important to examine the health-related SDGs beyond national-level estimates. As part of the Global Burden of Diseases, Injuries, and Risk Factors Study 2017 (GBD 2017), we measured progress on 41 of 52 health-related SDG indicators and estimated the health-related SDG index for 195 countries and territories for the period 1990-2017, projected indicators to 2030, and analysed global attainment.

Source: UG
Measuring progress of the millennium development goals. A fuzzy comprehensive evaluation approach

The United Nations’ target date to achieve its Millennium Development Goals (MDGs) to free people from extreme poverty and multiple deprivations is 2015. As the date approaches, several attempts have been made to evaluate the performance of the set goals, targets, and indicators. Although the methodology used to measure the performance of member countries has been questioned by many, the fiercest criticism so far has been the apparent lack of data with which to measure progress. The subjectivity of the MDGs further increases the complexity of the evaluation systems, but an effective measurability of each MDG is key to the overall success. This study applies a fuzzy comprehensive evaluation method (FCEM) in evaluating the performance of individual member countries with regard to the MDGs. The result shows how, in the absence of data, the FCEM can be used to evaluate performance of member countries involved in the MDG project.

Source: UG
Measuring progress of the millennium development goals: A fuzzy comprehensive evaluation approach

The United Nations' target date to achieve its Millennium Development Goals (MDGs) to free people from extreme poverty and multiple deprivations is 2015. As the date approaches, several attempts have been made to evaluate the performance of the set goals, targets, and indicators. Although the methodology used to measure the performance of member countries has been questioned by many, the fiercest criticism so far has been the apparent lack of data with which to measure progress. The subjectivity of the MDGs further increases the complexity of the evaluation systems, but an effective measurability of each MDG is key to the overall success. This study applies a fuzzy comprehensive evaluation method (FCEM) in evaluating the performance of individual member countries with regard to the MDGs. The result shows how, in the absence of data, the FCEM can be used to evaluate performance of member countries involved in the MDG project. Copyright © 2014 Taylor & Francis Group, LLC.

Source: UG
Measuring regional and district variations in the incidence of pregnancy-induced hypertension in Ghana: Challenges, opportunities and implications for maternal and newborn health policy and programmes

Most research activities involve the use of molecular methods such as regular PCR (PCR), quantitative PCR (Q-PCR), genomic sequence and analysis using different software, genetic engineering, probe hybridization techniques, biological and molecular cloning, evaluation of immune markers for laboratory diagnosis of infections, serological assays involving the use of rapid tests, ELISA-based evaluations and immunofluorescent assay techniques.

Source: UG
Measuring schistosomiasis case management of the health services in Ghana and Mali

The Department of Parasitology conducts research into parasitic diseases of public health importance with the overall goal of reducing their transmission and the heavy disease burden that they impose on affected populations. The Department maintains focus on parasitic diseases in general. These include major diseases such as malaria, and others listed under the Neglected Tropical Diseases (NTD) control initiative such as, lymphatic filariasis, onchocerciasis, schistosomiasis, soil-transmitted helminthiasis, trypanosomiasis and leishmaniasis.

Source: UG
Measuring sustainability of conservation and conventional practices in maize production in Ghana

Scientists and researchers worldwide have recommended Conservation Agriculture has the potential to promote sustainability in agriculture. This paper attempts to measure and compare the economic, social, and environmental sustainability of both conservation and conventional practices used for maize production in the northern region of Ghana employing the Multi-Attribute Value Theory (MAVT) approach. Based on the three pillars of sustainability (economic, social, and environmental), adopters of minimum tillage practice (scoring 0.5, 0.6, 0.82), and the combined adoption of minimum tillage and integrated organic-inorganic fertilizer application (scoring 0.53, 0.5, 0.88) emerged as sustainable practices. Despite these scores, the z-test of difference in means of the conservation practices and conventional ones were all insignificant, indicating that the conservation practices in use were not different from the conventional ones in terms of their contribution to sustainable maize production. Technical assistance and training that aids in ensuring the appropriate application of conservation practices should be ensured if the goal of sustainability is to be realized.

Source: UG