gebhart/patrepeval huggingface dataset:
Phenotype and progeny of primitive adherent human hematopoietic progenitors. (1996)

Hematopoietic progenitor cells can be classified as plastic- and stroma-adherent (P+S+), stroma-adherent (P-S+) and non-adherent (P-S-). Both P+S+ and P-S+ populations are detected in delta (delta) culture systems where they produce non-adherent (P-S-) granulocyte-macrophage colony-forming cells (CFU-GM) and erythroid burst-forming units (BFU-E). Here we demonstrate that the plastic-adherent progenitor cells (P delta cells) comprise 5-10 percent of the CD34+, population in adult human marrow. Moreover, they do not express CD3 or CD22 and 88 percent of them are CD38-, 88 percent are CD33- and 74 percent are HLA-DR-. Production of CFU-GM by purified plastic-adherent CD34+, adherent cells was 60 percent of the number produced by recombined CD34+, and CD34- fractions. We have shown also that the plastic-adherent P+S+ cells are the precursors of the stroma-adherent P-S+ cells (S delta cells), day 21 cobblestone-area forming cells (CAFC) and cells capable of sustained hematopoiesis in a modified long-term bone marrow culture system. These observations support the primitive nature of P delta cells and establish a phenotypic sequence of plastic and stroma adherence through stroma adherence to non-adherence in hematopoietic cell development. To further investigate the relationship between P delta cells, S delta cells and long-term culture-initiating cells (LTCIC), we cultured whole mononuclear cell tractions and plastic-adherent cell-depleted mononuclear cell fractions in long-term culture and in the S delta assay. The results indicated the P delta cells were inhibited in the presence of stromal cells.
Anagrelide‐Induced Cardiomyopathy (2000)

Anagrelide, a selective thrombocytopenic agent, is administered to treat a variety of hematologic disorders. Despite limited clinical experience with this drug, serious cardiovascular events, including congestive heart failure, have been reported. The proposed mechanism of cardiotoxicity is attributed to inhibition of phosphodiesterase, resulting in positive inotropic activity and vasodilation. A 48-year-old woman with polycythemia vera developed cardiotoxicity manifested by congestive heart failure and palpitations. It was suspected to be temporally related to titrating dosages of anagrelide.
Rac1 targeting suppresses p53 deficiency–mediated lymphomagenesis (2010)

Mutation of the p53 tumor suppressor is associated with disease progression, therapeutic resistance, and poor prognosis in patients with lymphoid malignancies and can occur in approximately 50% of Burkitt lymphomas. Thus, new therapies are needed to specifically target p53-deficient lymphomas with increased efficacy. In the current study, the specific impact of inhibition of the small GTPase Rac1 on p53-deficient B- and T-lymphoma cells was investigated. p53 deficiency resulted in increased Rac1 activity in both B-cell and T-cell lines, and its suppression was able to abrogate p53 deficiency-mediated lymphoma cell proliferation. Further, Rac targeting resulted in increased apoptosis via a p53-independent mechanism. By probing multiple signaling axes and performing rescue studies, we show that the antiproliferative effect of Rac1 targeting in lymphoma cells may involve the PAK and Akt signaling pathway, but not the mitogen-activated protein (MAP) kinase pathway. The effects of inhibition of Rac1 were extended in vivo where Rac1 targeting was able to specifically impair p53-deficient lymphoma cell growth in mouse xenografts and postpone lymphomagenesis onset in murine transplantation models. Because the Rac1 signaling axis is a critical determinant of apoptosis and tumorigenesis, it may represent an important basis for therapy in the treatment of p53-deficient lymphomas.
Epitope-based peptide vaccines predicted against novel coronavirus disease caused by SARS-CoV-2 (2020)

The outbreak of the 2019 novel coronavirus (SARS-CoV-2) has infected millions of people with a large number of deaths across the globe. The existing therapies are limited in dealing with SARS-CoV-2 due to the sudden appearance of the virus. Therefore, vaccines and antiviral medicines are in desperate need. We took immune-informatics approaches to identify B- and T-cell epitopes for surface glycoprotein (S), membrane glycoprotein (M) and nucleocapsid protein (N) of SARS-CoV-2, followed by estimating their antigenicity and interactions with the human leukocyte antigen (HLA) alleles. Allergenicity, toxicity, physiochemical properties analysis and stability were examined to confirm the specificity and selectivity of the epitope candidates. We identified a total of five B cell epitopes in RBD of S protein, seven MHC class-I, and 18 MHC class-II binding T-cell epitopes from S, M and N protein which showed non-allergenic, non-toxic and highly antigenic features and non-mutated in 55,179 SARS-CoV-2 virus strains until June 25, 2020. The epitopes identified here can be a potentially good candidate repertoire for vaccine development.
MUC4gene polymorphisms associate with endometriosis development and endometriosis-related infertility (2011)

MUC4 polymorphisms are associated with endometriosis development and endometriosis-related infertility in the Taiwanese population.
Cell wall changes in white spruce (<i>Picea glauca</i>) needles subjected to repeated drought stress (1991)

White spruce [ Picea glauca (Moench) Voss] seedlings were preconditioned by subjecting them to 3 cycles of a mild drought stress. After 1 week of stress relief their water status, soluble carbohydrate content and cell wall composition in newly formed needles were examined and compared with those in control seedlings. Both preconditioned and control seedlings were subsequently subjected to a severe drought stress and again analyzed. Preconditioning treatment both before and during subsequent stress exposure lowered osmotic potentials at full hydration, and after the loss of turgor, decreased lignin content and increased hemicellulose content of the cell walls. Severe drought had similar but more drastic effects on seedling water relations, sugar accumulation and cell wall hemicellulose content; it also decreased cell wall pectin levels. The decrease in pectin levels was accompanied by a loss of galactose and glucose from pectic substances. Little change in cellulose content was observed as a result of preconditioning and severe drought.
Amino Acid Composition and Flavor of Fresh Market Tomatoes as Influenced by Fruit Ripeness When Harvested1 (1978)

Abstract Four amino acids (glutamic, γ-aminobutyric, glutamine, and aspartic) make up about 80% of the total free amino acids in fruits of tomato ( Lycopersicon esculentum Mill., cv. Cal Ace). Fruits harvested at the table-ripe stage contained more alanine and less glutamic acid than those picked green or at the breaker (incipient red color) stage and ripened at 20°C to table-ripe. The higher glutamic acid concentrations in fruit picked at the breaker or earlier stages were paralled to higher scores for “off-flavor,” as described by a taste panel, relative to fruits picked table-ripe. However, when monopotassium glutamate (60, 120, or 180 mg/l00g) was added to diced table-ripe fruits, the panelists were not able to detect flavor differences due to increased glutamic acid concn. Differences in amino acid composition associated with fruit ripeness when picked do not appear to be directly related to flavor differences.
Sox2 roles in neural stem cells (2009)

Throughout vertebrate evolution, Sox2 marks the developing nervous system from its earliest developmental stages and, therein, the most undifferentiated precursor cells, including stem cells. Recent gene targeting studies investigated the function of Sox2 in two neuronal systems: the developing eye and brain. These studies uncovered a requirement for Sox2 in the maintenance of neural stem cells, as well as a downstream role in the differentiation of specific neuron sub-types. In both systems, Sox2 action is markedly dose-dependent, and downstream-target gene studies are beginning to reveal the mechanisms of Sox2 function.
Adiabatic Threshold Inverter Quantizer for a 3-bit Flash ADC (2016)

A novel Threshold Inverter Quantizer (TIQ) is suggested in the work to implement a 3-bit, 1.2V Flash Analog to Digital Converter (ADC). Two Phase Adiabatic Static CMOS Logic (2PASCL) is used for designing of Threshold Inverter Quantizer (TIQ) Comparator. Suggested work is carried out with SMIC 65nm Technology and simulated on Empyrean EDA tool. There is a need of different reference voltage for operation of comparator and this is achieved by systematically sizing the transistors of the TIQ comparators. The total power dissipation for the proposed ADC simulated at 1.2V Vdd and for 1fF capacitive load at 1.2 Hz, 1 KHz, 1 MHz input frequency is observed as 1.69 μW, 1.79 μW, 2.26 μW respectively; which is about 89% lesser than that of conventional CMOS TIQ based Flash ADC design.
Health problems associated with consumption of fish and the role of aquatic environments in the transmission of human diseases. (2012)

The majority of the numerous fish parasites are harmless to man and many domestic animals because when eaten with their fish hosts, they are digested. However, some of the fish parasites with larval stages in freshwater or marine teleosts have zoonotic potential if eaten raw or partially cooked. These are usually parasites, which have a piscivorous mammalian carnivore as their normal final host and are able to infect man because of the low host specificity of the adult stage. The major groups of fish parasite that are known as potentially dangerous pathogens of man belong to the helminth groups cestoda, trematoda, nematoda and rarely acanthocephala. However, bacterial and viral disease of man transmitted through fish are not uncommon. Toxic substances, metals and insecticides used to control human diseases in aquatic environments may accumulate in fish in po1lluted waters at such levels as to constitute a health risk to the consumer. Other health problems associated with fish arise from its perishable nature for example, in adequate handling, processing and storage, which may lead to the accumulation of microbes enhancing the risk of food poisoning. The aquatic environment in Africa constitutes a breeding habitat to several vectors of human diseases such as mosquitoes, snails and black flies. This paper reviews the role played by fish in transmitting diseases to humans as well as the importance of the aquatic environments in the transmission of human diseases such as Malaria, Schistosomiasis and onchocerciasis.
Challenges assessing clinical endpoints in early Huntington disease (2010)

The basic aim of this study was to evaluate the current accepted standard clinical endpoint for the earliest-studied HD participants likely to be recruited into clinical trials. As the advent of genetic testing for HD, it is possible to identify gene carriers before the diagnosis of disease, which opens up the possibility of clinical trials of disease-modifying treatments in clinically asymptomatic persons. Current accepted standard clinical endpoints were examined as part of a multinational, 32-site, longitudinal, observational study of 786 research participants currently in the HD prodrome (gene-positive but not clinically diagnosed). Clinical signs and symptoms were used to prospectively predict functional loss as assessed by current accepted standard endpoints over 8 years of follow-up. Functional capacity measures were not sensitive for HD in the prodrome; over 88% scored at ceiling. Prospective evaluation revealed that the first functional loss was in their accustomed work. In a survival analysis, motor, cognitive, and psychiatric measures were all predictors of job change. To our knowledge, this is the first prospective study ever conducted on the emergence of functional loss secondary to brain disease. We conclude that future clinical trials designed for very early disease will require the development of new and more sensitive measures of real-life function.
Ruminal Lipopolysaccharide Concentration and Inflammatory Response During Grain-Induced Subacute Ruminal Acidosis in Dairy Cows (2007)

The effects of grain-induced subacute ruminal acidosis (SARA) in lactating dairy cows on free ruminal lipopolysaccharide (LPS) and indicators of inflammation were determined. Four mid lactation dairy cows were divided into 2 groups of 2 cows and used in a repeated switchover design. During each period, SARA was induced in 2 animals for 5 subsequent days by replacing 25% of their total mixed ration (dry matter basis) with a concentrate made of 50% wheat and 50% barley. The other 2 cows acted as controls and were fed a total mixed ration diet in which 44% of dry matter was concentrate. On average, inducing SARA did not affect milk composition, increased the duration of rumen pH below 5.6 from 187 to 309 min/d, and increased free ruminal LPS concentration from 24,547 endotoxin units (EU)/mL to 128,825 EU/mL. Averaged across treatments, milk fat yield and milk protein yield were 0.66 and 1.00 kg/d, respectively. Rumen pH and milk fat data suggest that control cows also experienced ruminal acidosis, albeit a milder form of this disease than SARA cows. Serum LPS concentration in both control and SARA cows was less than the detection limit of <0.01 EU/mL for the assay. Induction of SARA elevated serum amyloid A concentration from 286.8 to 498.8 mug/mL, but did not affect other markers of inflammation including haptoglobin, fibrinogen, serum copper, or white blood cells. These results suggest that grain-induced SARA in mid lactation dairy cows increases the lysis of gram-negative bacteria and activates an inflammatory response.
Development of MEG Regeneration Unit Compatible Corrosion Inhibitor for Wet Gas Systems (2012)

Abstract Transportation of hydrocarbons and water in long subsea flow lines from satellite fields to a platform or to an onshore facility presents new challenges in the control of gas hydrates, corrosion, and mineral scale. Gas hydrates form at high pressure and low temperature and are a common problem in offshore wet gas pipelines due to low seabed temperatures and elevated pressures in these remote subsea developments. Monoethylene glycol (MEG) is widely used as a thermodynamic hydrate inhibitor in these developments to manage the risk of hydrate formation during production and transportation of multiphase fluids from subsea wells. Due to large amounts of MEG required for effective hydrate control, it is necessary to recycle and re-use it. The main processes for recycling of MEG are regeneration and reclamation. Typical conditions of regeneration and reclamation processes are ambient to vacuum pressures and temperatures in the range of 120°C −150°C1. In addition to the use of MEG for hydrate control, corrosion inhibitors are also applied for corrosion control in the subsea pipelines and infrastructure. These corrosion inhibitors must be able to perform under high shear and highly corrosive environments without losing their effectiveness after having been subjected to the system conditions present in the MEG regeneration process. Inappropriate selection of corrosion inhibitors for MEG based applications can lead to severe fouling/formation of solids, emulsion and foaming issues in the receiving facilities. The corrosion inhibitors developed for use in facilities operating with glycol regeneration systems should remain active after multiple MEG Regeneration Unit (MRU) cycles without causing fouling/formation of solids, emulsion and foaming. The current paper presents MRU compatible corrosion inhibitors developed based on the stringent testing methods adopted from real time MRU process.
Platelet-derived growth factor expression in mesangial proliferative glomerulonephritis. (1991)

Proliferation of mesangial cells and expansion of mesangial matrix are common histologic features of proliferative glomerular disease, a frequent cause of renal failure. Proliferation of glomerular mesangial cells occurs in response to platelet-derived growth factor (PDGF), and these cells release PDGF and express PDGF A and B chain mRNAs. However, all studies relating PDGF to potential changes in glomerular structure and function to date have been performed in vitro. To explore the role of PDGF in proliferative glomerulonephritides, we studied the expression of PDGF in vivo in two animal models of IgA nephropathy with different histologic patterns of glomerular injury: either predominant mesangial proliferation or expansion of mesangial matrix. Increased expression of PDGF and PDGF B-chain mRNA in whole kidneys from diseased mice was demonstrated by immunohistochemical techniques and by solution hybridization assay, respectively. Immunohistochemically, PDGF was localized primarily within the mesangial area of glomeruli and to a much lower extent in interstitium. The increased PDGF expression correlated with the degree of hypercellularity and clinical features of the disease. In addition, PDGF expression was increased in some forms of human glomerulonephritis, characterized by mesangial proliferation. These findings suggest that PDGF may be a major contributor to mesangial cell proliferation seen in proliferative glomerulonephritides.
<i>Actinobacillus actinomycetemcomitans</i> Cytolethal Distending Toxin (Cdt): Evidence That the Holotoxin Is Composed of Three Subunits: CdtA, CdtB, and CdtC (2004)

We have shown the Actinobacillus actinomycetemcomitans produces an immunosuppressive factor encoded by the cytolethal distending toxin (cdt)B gene, which is homologous to a family of Cdts expressed by several Gram-negative bacteria. We now report that the capacity for CdtB to induce G(2) arrest in Jurkat cells is greater in the presence of the other Cdt peptides: CdtA and CdtC. Plasmids containing the cdt operon were constructed and expressed in Escherichia coli; each plasmid contained a modified cdt gene that expressed a Cdt peptide containing a C-terminal His tag. All three Cdt peptides copurified with the His-tagged Cdt peptide. Each of the peptides associated with the complex was truncated; N-terminal amino acid analysis of CdtB and CdtC indicated that the truncation corresponds to cleavage of a previously described signal sequence. CdtA was present in two forms in crude extracts, 25 and 18 kDa; only the 18-kDa fragment copurified with the Cdt complexes. Cdt complexes were also immunoprecipitated from A. actinomycetemcomitans extracts using anti-CdtC mAb. Exposure of Jurkat cells to 40 pg resulted in >50% accumulation of G(2) cells. CdtB and CdtC were detected by immunofluorescence on the cell surface after 2-h exposure to the holotoxin. CdtA was not detected by immunofluorescence, but all three peptides were associated with Jurkat cells when analyzed by Western blot. These studies suggest that the active Cdt holotoxin is a heterotrimer composed of truncated CdtA, CdtB, and CdtC, and all three peptides appear to associate with lymphocytes.
Intracavitary administration of radioactive isotopes in the control of effusions due to cancer:Results in 267 patients (1966)

This presents a series of 267 patients given a total of 325 treatments of radioactive isotopes administered intracavum in the control of ascites and hydrothorax. The following radioactive isotopes were employed: Radioactive gold (Au198), radioactive chromic phosphate (CrP32O4), radioactive AU198 and CrP32O4 in combination and radioactive yttrium (Y90). The intracavitary instillation of radioactive Au198 and/or CrP32O4 afforded 54% of patients some degree of salutary benefit. Of 99 patients with hydrothorax 5.0% had an excellent response to treatment and 24% a good response; pleural effusion from a primary cancer of the breast, lung and lymphoma showed the most satisfactory response. Of 145 patients with cancerous ascites, 54% received benefit, 12% showing excellent response and 19% showing good response; ascites due to a primary cancer in the female genital tract (especially the ovary), the gastrointestinal tract and the lymphomas enjoyed the best palliation. Cancerous effusion carries an ominous prognosis; the average duration of life in our treated series was 6.3 months. In contrast, patients with effusion due to lymphoma survived an average period of 15 months. In half the patients treated, an undetermined restraint in fluid formation was noted with a probable resultant indeterminate prolongation of life. Complications were minimal.
COMMON-Sense Net: Improved Water Management for Resource-Poor Farmers via Sensor Networks (2006)

We describe the on-going design and implementation of a sensor network for agricultural management targeted at resource-poor farmers in India. Our focus on semi-arid regions led us to concentrate on water-related issues. Throughout 2004, we carried out a survey on the information needs of the population living in a cluster of villages in our study area. The results highlighted the potential that environment-related information has for the improvement of farming strategies in the face of highly variable conditions, in particular for risk management strategies (choice of crop varieties, sowing and harvest periods, prevention of pests and diseases, efficient use of irrigation water etc.). This leads us to advocate an original use of Information and Communication Technologies (ICT). We believe our demand-driven approach for the design of appropriate ICT tools that are targeted at the resource-poor to be relatively new. In order to go beyond a pure technocratic approach, we adopted an iterative, participatory methodology
Lung Nodule Retrieval System (2013)

Early detection and removal of pulmonary nodules significantly improves long term survival rates for patients with lung cancer. This paper provides the overview of different methods used in the retrieval system of lung nodules by a comprehensive review of existing literature. Firstly, the high level features of DICOM CT images are used for retrieval of filtered lung images from the database. The preprocessing step is used for separation of lungs fields on the filtered images. Linear Binary Pattern extracts the low level features from extracted lung areas to perform the segmentation. The technique of template matching further uses to retrieve the abnormal nodules from Lung data set.
Development of HTS-SQUID System for a Monitoring System of CO2 Enhanced Oil Recovery (2016)

We have tried to develop an induction logging system for oil fields utilizing a high-temperature superconducting quantum interference device (HTS-SQUID). Enhanced oil recovery (EOR) technology utilizing carbon dioxide (CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) is a promising technique for efficient production of petroleum. To control the EOR, it is important to improve the accuracy of monitoring technique of CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> injected into the oil reservoir. The HTS-SQUID has a potential to monitor CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> behavior in crude oil reservoir because of its high sensitivity in a wide frequency range from dc to over 1 MHz. As the first step of the developments, we prepared a vessel for SQUID system by using carbon fiber reinforced plastic with pressure tightness of 70 MPa and thermal durability of 200 °C. By using the SQUID system placed at a depth of about 300 m in a steel-cased borehole filled with water, magnetic signals from a transmitter coil in another borehole were successfully measured.