gebhart/patrepeval huggingface dataset:
Purification and characterization of the nickel-containing multicomponent urease from Klebsiella aerogenes. (1987)

Klebsiella aerogenes urease was purified 1,070-fold with a 25% yield by a simple procedure involving DEAE-Sepharose, phenyl-Sepharose, Mono Q, and Superose 6 chromatographies. The enzyme preparation was comprised of three polypeptides with estimated Mr = 72,000, 11,000, and 9,000 in a alpha 2 beta 4 gamma 4 quaternary structure. The three components remained associated during native gel electrophoresis, Mono Q chromatography, and Superose 6 chromatography despite the presence of thiols, glycols, detergents, and varied buffer conditions. The apparent compositional complexity of K. aerogenes urease contrasts with the simple well-characterized homohexameric structure for jack bean urease (Dixon, N. E., Hinds, J. A., Fihelly, A. K., Gazzola, C., Winzor, D. J., Blakeley, R. L., and Zerner, B. (1980) Can. J. Biochem. 58, 1323-1334); however, heteromeric subunit compositions were also observed for the enzymes from Proteus mirabilis, Sporosarcina ureae, and Selemonomas ruminantium. K. aerogenes urease exhibited a Km for urea of 2.8 +/- 0.6 mM and a Vmax of 2,800 +/- 200 mumol of urea min-1 mg-1 at 37 degrees C in 25 mM N-2-hydroxyethylpiperazineN'-2-ethanesulfonic acid, 5.0 mM EDTA buffer, pH 7.75. The enzyme activity was stable in 1% sodium dodecyl sulfate, 5% Triton X-100, 1 M KCl, and over a pH range from 5 to 10.5, with maximum activity observed at pH 7.75. Two active site groups were defined by their pKa values of 6.55 and 8.85. The amino acid composition of K. aerogenes urease more closely resembled that for the enzyme from Brevibacter ammoniagenes (Nakano, H., Takenishi, S., and Watanabe, Y. (1984) Agric. Biol. Chem. 48, 1495-1502) than those for plant ureases. Atomic absorption analysis was used to establish the presence of 2.1 +/- 0.3 mol of nickel per mol of 72,000-dalton subunit in K. aerogenes urease.
Bad Foundation and its Treatment of HeBei Coastal Highway (2015)

In recent years, with the accelerating urbanization process, the national road network continues to expand, a lot of highway construction projects are in the countryside. The geological properties of high level, long distance highway foundation are complicated. In order to guarantee the stability of highway embankment, we need to deal with bad foundation. And the same project may use several different forms of foundation treatment methods, each method has its own characteristics and the optimal conditions shall apply. After the start of construction, as the stratum situations of the construction slot are further revealed that designers often have to dynamically adjust foundation treatment scheme according to actual conditions. In this paper, based on the Hebei Qinhuangdao, Tangshan coastal highway road construction as an example, we briefly expounded the engineering geology, the characteristics of soft soil foundation, geological properties and engineering treatment methods.
Interactions between STAT and Non-STAT Proteins in the Interferon-Stimulated Gene Factor 3 Transcription Complex (1996)

The first STAT-containing transcription factor to be studied, the alpha-interferon-induced ISGF3, is composed of a Stat1:2 heterodimer and a weak DNA-binding protein, p48, that is a member of a growing family of proteins similar to the so-called interferon regulatory factor (IRF-1). The p48 and Stat1:2 heterodimer do not associate stably in the absence of DNA, but we show that amino acids approximately 150 to 250 of Stat1 and a COOH-terminal portion of p48 exhibit physical interaction, implying contact that stabilizes ISGF3. Moreover, amino acid exchanges within the Stat1 contact region diminish or abolish the functional activity of Stat1. This protein interaction domain may be important in other STAT proteins to recruit partners to multiprotein transcription factors.
Writing about Writing (2012)

Although the correspondents reflected about the writing-thinking-doing-learning process at many points in their letters, this section of the book focuses on the contributions that specifically deal with writing, at some length.
The Effects of Human GH and Its Lipolytic Fragment (AOD9604) on Lipid Metabolism Following Chronic Treatment in Obese Mice andβ 3-AR Knock-Out Mice (2001)

Both human GH (hGH) and a lipolytic fragment (AOD9604) synthesized from its C-terminus are capable of inducing weight loss and increasing lipolytic sensitivity following long-term treatment in mice. One mechanism by which this may occur is through an interaction with the beta-adrenergic pathway, particularly with the beta(3)-adrenergic receptors (beta(3)-AR). Here we describe how hGH and AOD9604 can reduce body weight and body fat in obese mice following 14 d of chronic ip administration. These results correlate with increases in the level of expression of beta(3)-AR RNA, the major lipolytic receptor found in fat cells. Importantly, both hGH and AOD9604 are capable of increasing the repressed levels of beta(3)-AR RNA in obese mice to levels comparable with those in lean mice. The importance of beta(3)-AR was verified when long-term treatment with hGH and AOD9604 in beta(3)-AR knock-out mice failed to produce the change in body weight and increase in lipolysis that was observed in wild-type control mice. However, in an acute experiment, AOD9604 was capable of increasing energy expenditure and fat oxidation in the beta(3)-AR knock-out mice. In conclusion, this study demonstrates that the lipolytic actions of both hGH and AOD9604 are not mediated directly through the beta(3)-AR although both compounds increase beta(3)-AR expression, which may subsequently contribute to enhanced lipolytic sensitivity.
Ultratrace Enrichment of Tyrosine Phosphorylated Peptides on an Imprinted Polymer (2011)

Novel molecularly imprinted polymers (MIPs) designed to bind the side chain of phosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO(2)-based methods, the pTyr-imprinted polymers offered high selectivity for pTyr-containing peptides down to the low fmol level. This suggests MIPs as a new tool for affinity-based proteomics.
Mosaic Generation in H.264 Compressed Domain (2006)

This paper presents an efficient approach to mosaic generation in H.264 compressed domain. The proposed mosaic generation approach fully exploits several new coding characteristics of the H.264 standard, i.e., variable block size and the quarter-pel accuracy of motion vector (MV). A uniformly sampled and normalized MV field is first derived from the H.264 compressed video. Then an affine model is used to estimate the global motion from the normalized MV field, and the model parameters are iteratively refined based on outlier rejection scheme. Finally, the most recent and reliable background pixels from multiple frames are warped to the reference frame and then used to integrate the final mosaic. Experimental results demonstrate that the proposed approach is efficient for mosaic generation in H.264 compressed domain.
TiO<sub>2</sub> Inverse Opals Fabricated Using Low‐Temperature Atomic Layer Deposition (2005)

High-quality inverse-opal photonic crystals (see Figure) are formed by TiO2 infiltration and subsequent removal of SiO2 opal templates. Low-temperature atomic layer deposition followed by annealing yields highly conformal infiltrations with extremely smooth surfaces (less than 1 nm root-mean-square roughness) and filling fractions of ∼ 88 %. Photonic-bandgap properties were determined using UV-vis reflectivity and transmission measurements.
Application of a floating well concept to a latch-up-free, low-cost, smart power high-side switch technology (1993)

An original design methodology that permits implementing latch-up-free smart power circuits on a very simple, cost-effective technology is presented. The basic concept used for this purpose is letting float the wells of the MOS transistors most susceptible to initiate latch-up.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Pesticide Biotransformation in Plants and Microorganisms (2000)

Understanding pesticide metabolism in plants and microorganisms is a key component for the development, the safe and efficient utilization of these compounds, and for bioremediation of these chemicals in contaminated soil and water. Selective metabolism of pesticides in non-target species (e.g., crop plants) and sensitivity in target species (e.g. weeds, insects and pathogen pests), is the basis of chemical pest control. Pesticide biotransformations may occur via metabolism or co-metabolism. Metabolism of a given pesticide in plants and microorganisms is generally a multi-step process. Individual components of such degradation/detoxification pathways include: oxidation, reduction, hydrolysis and conjugation. Pathway diversity depends on the chemical structure of the xenobiotic compound, the organism, environmental conditions, and metabolic factors regulating expression of these biochemical pathways. Knowledge of these enzymatic processes, especially concepts related to mechanism of action, resistance, selectivity, tolerance, and environmental fate has advanced pesticide science. One example is the development of herbicide tolerant crops. Advances in pesticide metabolism have also been facilitated by use of improved analytical techniques, molecular biological approaches, and immunological tools.
A Hybrid ARQ Scheme using Packet Error Prediction on OFDM based HSDPA Systems (2007)

Next generation mobile communication services require high throughput and wide bandwidth at high mobile speed. This is made possible with the help of strong channel coding methods such as turbo codes, hybrid ARQ (HARQ) schemes, and channel equalization techniques. But turbo decoding is complex and takes a large part of processing time and power consumption of user equipments (UE). Therefore, the HARQ scheme related to turbo decoding has great effects on the system performance. This paper proposes an enhanced HARQ scheme that can be used in orthogonal frequency division multiplexing (OFDM) based high speed downlink packet access (HSDPA) systems. The proposed scheme uses the threshold which comes from packet error prediction to skip unnecessary turbo decoding process. With the proposed scheme, the power consumption of UE can be reduced, while increasing the performance of the system.
Mechanisms of intermolecular homologous recombination in plants as studied with single- and double-stranded DNA molecules (1992)

To elucidate the mechanism for intermolecular homologous recombination in plants we cotransformed Nicotiana tabacum cv Petit Havana SR1 protoplasts with constructs carrying different defective derivatives of the NPTII gene. The resulting kanamycin resistant clones were screened for possible recombination products by PCR, which proved to be a valuable technique for this analysis. Our results show that the double-stranded circular DNA molecules used in this study recombine predominantly via a pathway consistent with the single-strand annealing (SSA) model as proposed for extrachromosomal recombination in mammalian cells. In the remaining cases recombination occurred via a single reciprocal recombination, gene conversion and possibly double reciprocal recombination. Since single-stranded DNA is considered to be an important intermediate in homologous recombination we also established the recombination ability of single-stranded DNA in intermolecular recombination. We found that single-stranded DNA enters in recombination processes more efficiently than the corresponding double-stranded DNA. This was also reflected in the recombination mechanisms that generated the functional NPTII gene. Recombination between a single-stranded DNA and the complementing DNA duplex occurred at similar rates via a single reciprocal recombination and the SSA pathway.
Acquisition and processing of point source measurements in land seismic (2000)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 2000Acquisition and processing of point source measurements in land seismicAuthors: G. J. M. BaetenV. BelougneM. DalyB. JeffryesJ. E. MartinG. J. M. BaetenSchlumberger, V. BelougneSchlumberger, M. DalySchlumberger, B. JeffryesSchlumberger, and J. E. MartinSchlumbergerhttps://doi.org/10.1190/1.1816095 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.1816095FiguresReferencesRelatedDetailsCited byISS on ice: Seismic acquisition in the arcticJan Kommedal, Gino Alexander, Sean Wagner, and Lawrence Wyman1 September 2016Suppression of near-surface scattered body-to-surface waves: a steerable and nonlinear filtering approach27 July 2015 | Geophysical Prospecting, Vol. 64, No. 2Interpretation of full-azimuth broadband land data from Saudi Arabia and implications for improved inversion, reservoir characterization, and explorationBrian P. Wallick and Luis Giroldi14 October 2013 | Interpretation, Vol. 1, No. 2Integration of surface/subsurface techniques reveals faults in Gulf of Suez oilfields27 April 2011 | Petroleum Geoscience, Vol. 17, No. 2On the accuracy of the ground force estimated in vibroseis acquisitionGeophysical Prospecting, Vol. 58, No. 1Orthogonal vibroseis sweepsGeophysical Prospecting, Vol. 53, No. 5Cost‐effective, high‐density vibroseis acquisitionJulien Meunier and Thomas Bianchi7 December 2005An integrated 3D acquisition and processing technique using point sources and point receiversJohn Quigley3 January 2005 SEG Technical Program Expanded Abstracts 2000ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 2000 Pages: 2484 publication data© 2000 Copyright © 2000 Society of Exploration GeophysicistsPublisher:Society of Exploration Geophysicists HistoryPublished Online: 04 Jan 2005 CITATION INFORMATION G. J. M. Baeten, V. Belougne, M. Daly, B. Jeffryes, and J. E. Martin, (2000), "Acquisition and processing of point source measurements in land seismic," SEG Technical Program Expanded Abstracts : 45-48. https://doi.org/10.1190/1.1816095 Plain-Language Summary PDF DownloadLoading ...
Orale Kontrastmittel für die magnetische Resonanztomographie des Abdomens (1987)

Beagle dogs were investigated by MRI (Siemens Magnetom, 0.35 T) after oral administration of aqueous solutions of Gd-DTPA (n = 4), ferric ammonium citrate (n = 1), and magnetite dextran (n = 2). High signal intensity of the GI-tract versus adjacent structures was obtained with Gd-DTPA and ferric ammonium citrate. Magnetite showed negative contrast versus adjacent structures. However, magnetite displayed lower contrast relative to liver, muscle and gut wall compared to Gd-DTPA and ferric ammonium citrate. In relatively T2-weighted sequences labeling of poorly filled bowel loops was significantly better with Gd-DTPA and ferric ammonium citrate. For GI-tract contrast enhancement we conclude that positive contrast media are of higher diagnostic value compared to negative contrast agents.
TWO CELLULAR ARCHITECTURES FOR INTEGRATED IMAGE SENSING AND PROCESSING ON A SINGLE CHIP (1998)

Two architectures for a programmable image processor with on-chip light sensing capability are described. The first is a VLSI implementation of a cellular neural network. The second is a distributed dual-structure mutation of the first architecture. The distributed dual architecture leverages the speed of silicon against the large silicon area requirements. Moreover, the innovative integrated nature of the dual-structure design significantly reduces the bottleneck and computational overload caused by data transfer from sensory focal plane to the image processor. The paper also describes VLSI chip prototypes and test results.
Lotus Notes and collaboration: le plus c/spl cedil/a change (1996)

Work in organizations is becoming increasingly focused on collaborative work in groups. Groupware is widely touted as the information technology that can support this new mode of work by fostering collaboration. In a study of Lotus Notes, a popular groupware product, implemented throughout the professional staff of a large American insurance company, we found the impact of groupware to be somewhat different from what many might have expected. While almost everyone was quite pleased with the Notes implementation and its perceived impact, there was no evidence of a change in the degree of collaboration among organization members. Two key themes are explored as possible explanations for this result: the fit of the technology to the organization, and limited training in how best to use this new technology.
Roles of Beta2- and Beta3-Adrenoceptor Polymorphisms in Hypertension and Metabolic Syndrome (2010)

Hypertension, diabetes mellitus (especially type 2 diabetes mellitus), metabolic syndrome and obesity are rapidly growing public health problems. Sympathetic nerve activation is observed in obesity, hypertension and diabetes mellitus, which have strong genetic as well as environmental determinants. Reduced energy expenditure and resting metabolic rate are predictive of weight gain, and the sympathetic nervous system participates in regulating energy balance through thermogenesis. The thermogenic effects of catecholamines in obesity have been mainly mediated via the β2- and β3-adrenergic receptors in humans. Further, β2-adrenoceptors importantly influence vascular reactivity and may regulate blood pressure. Genetic polymorphistns of the β-adrenoceptor gene have been shown to alter the function of several adrenoceptor subtypes and thus to modify the response to catecholamine. β2-adrenoceptor polymorphisms (Arg16Gly, Gln27Glu, and Thr164Ile) have been studied in relation to hypertension. Genetic variations in the β3-adrenoceptor (i.e. Try64Arg variant) are also associated with both obesity and hypertension. However, the precise relationships of the polymorphisms of β2- and β3-adrenoceptor genes with sympathetic nervous system activity, hypertension, and metabolic syndrome have not been fully clarified. This paper will discuss the current topics involving the influence of the sympathetic nervous system and β2- and β3- adrenoceptor polymorphisms in hypertension and metabolic syndrome.
CD23/CD21 interaction is required for presentation of soluble protein antigen by lymphoblastoid B cell lines to specific CD4<sup>+</sup> T cell clones (1994)

Previous studies have documented a role for membrane-bound CD23 (the low affinity Fc epsilon RII) in presentation of alloantigens by B cells. The aim of the present study was to examine the involvement of cell surface CD23 in presentation of more conventional soluble protein antigens to T cells. We show that antibodies to CD23 and to its lymphocyte-associated second ligand, CD21, inhibit presentation of the cow's milk allergen casein, by autologous CD23+CD21+ B-EBV cell lines to casein-specific HLA-DP-restricted CD4+ T cell clones obtained from patients with either reaginic or enterophatic forms of cow's milk protein intolerance. Maximal inhibition was achieved when the antibodies were added at the initiation of the culture. The absence of specific inhibition by an anti-DR alpha monoclonal antibody (mAb) argues against a steric hindrance phenomenon impeding access of the T cell receptor to major histocompatibility complex class II molecules. Rather, anti-CD23 and anti-CD21 mAb-induced inhibition of antigen presentation seems to affect at least partly, heterotypic conjugate formation through CD23/CD21 interaction. Double immunofluorescence labeling of the T cell clones and antibody inhibition of T/B conjugate formation shows that functional CD23 and CD21 molecules are induced on T cells following contact with B-EBV cell lines. Taken together, these data indicate that CD23/CD21 interactions between T and B cells are required for presentation of soluble protein antigens by B-EBV cell lines to specific CD4+ T cells. The potential implications of these findings for allergen-specific T cell activation are discussed.
Comparison of Botox with a Chinese type A botulinum toxin. (2000)

Both preparation are safe and effective treatments for patients with focal dystonia and muscle spasm. They both have subclinical effects on neuromuscular transmission of remote uninjected muscles. The Chinese preparation is a little less powerful but much cheaper than Botox.
The Future of Fibromyalgia: (1995)

Objectives: To identify issues of importance regarding the validitiy of the fibromyalgia construct, its pathogenesis, treatment and outcome. Findings: Major unresolved iddues regarding construct, diagnosis, pathogenesis, treatment effect and outcome exist. Confusion between epiphenomena and pathogenetic abnormalities are common. Therapy has not been effective though statistically significant benefit has been demonstrated. Conclusion: Future research needs to consider fibromyalgia as a continuum rather than a discrete syndrome or "disease." Pathogenetic studies need to consider psychological factors more prominently. Longitudinal studies of outcome are important. New outcome measures need to be developed.
The universal dielectric response and its physical significance (1992)

The dielectric response of virtually all solid dielectrics follows fractional power laws in frequency and in time and these call for a fresh appraisal of the physical significance of these laws. A brief overview of the experimental situation in a wide range of systems is given, and a number of possible interpretations which depart radically from the traditional picture of independently relaxing entities are discussed. It is suggested that a new consensus is appearing for the understanding of the underlying processes leading to the observed universality.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Enantioselective Synthesis of the Aglycones of Pseudopterosin and <i>seco</i>-Pseudopterosin via a Common Synthetic Intermediate (1997)

pseudopterosin - enantioselective synthesis - benzylic stereocontrol - chiral arene-Cr(CO)3 complexes