gebhart/patrepeval huggingface dataset:
Regulation of histone mRNA in the unperturbed cell cycle: evidence suggesting control at two posttranscriptional steps. (1991)

The levels of histone mRNA increase 35-fold as selectively detached mitotic CHO cells progress from mitosis through G1 and into S phase. Using an exogenous gene with a histone 3' end which is not sensitive to transcriptional or half-life regulation, we show that 3' processing is regulated as cells progress from G1 to S phase. The half-life of histone mRNA is similar in G1- and S-phase cells, as measured after inhibition of transcription by actinomycin D (dactinomycin) or indirectly after stabilization by the protein synthesis inhibitor cycloheximide. Taken together, these results suggest that the change in histone mRNA levels between G1- and S-phase cells must be due to an increase in the rate of biosynthesis, a combination of changes in transcription rate and processing efficiency. In G2 phase, there is a rapid 35-fold decrease in the histone mRNA concentration which our results suggest is due primarily to an altered stability of histone mRNA. These results are consistent with a model for cell cycle regulation of histone mRNA levels in which the effects on both RNA 3' processing and transcription, rather than alterations in mRNA stability, are the major mechanisms by which low histone mRNA levels are maintained during G1.
Surface passivation of silicon solar cells using industrially relevant Al2O3 deposition techniques (2010)

The next generation of industrial silicon solar cells aims at efficiencies of 20% and above. To achieve this goal using ever-thinner silicon wafers, a highly effective surface passivation of the cell front and rear is required. In the past, finding a suitable dielectric layer providing a high-quality rear passivation has been a major challenge. Aluminium oxide (Al2O3) grown by atomic layer deposition (ALD) has only recently turned out to be a nearly perfect candidate for such a dielectric. However, conventional ALD is limited to deposition rates well below 2nm/min, which is incompatible with industrial solar cell production. This paper assesses the passivation quality provided by three different industrially relevant techniques for the deposition of Al2O3 layers, namely high-rate spatial ALD, plasma-enhanced chemical vapour deposition (PECVD) and reactive sputtering.
Semitone frequency mapping to improve music representation for nucleus cochlear implants (2011)

The frequency-to-channel mapping for Cochlear implant (CI) signal processors was originally designed to optimize speech perception and generally does not preserve the harmonic structure of music sounds. An algorithm aimed at restoring the harmonic relationship of frequency components based on semitone mapping is presented in this article. Two semitone (Smt) based mappings in different frequency ranges were investigated. The first, Smt-LF, covers a range from 130 to 1502 Hz which encompasses the fundamental frequency of most musical instruments. The second, Smt-MF, covers a range from 440 to 5040 Hz, allocating frequency bands of sounds close to their characteristic tonotopical sites according to Greenwood's function. Smt-LF, in contrast, transposes the input frequencies onto locations with higher characteristic frequencies. A sequence of 36 synthetic complex tones (C3 to B5), each consisting of a fundamental and 4 harmonic overtones, was processed using the standard (Std), Smt-LF and Smt-MF mappings. The analysis of output signals showed that the harmonic structure between overtones of all complex tones was preserved using Smt mapping. Semitone mapping preserves the harmonic structure and may in turn improve music representation for Nucleus cochlear implants. The proposed semitone mappings incorporate the use of virtual channels to allow frequencies spanning three and a half octaves to be mapped to 43 stimulation channels. A pitch difference limen test was done with normal hearing subjects discriminating pairs of pure tones with different semitone intervals which were processed by a vocoder type simulator of CI sound processing. The results showed better performance with wider semitone intervals. However, no significant difference was found between 22 and 43 channels maps.
Integer sequences, online encyclopedia of (2003)

The Online Encyclopedia of Integer Sequences (http://www.research.att.com/~njas/sequences/) is a large database of number sequences. Three examples will illustrate some of the ways in which it has been used.
Sequence of plasmid pGT5 from the archaeon Pyrococcus abyssi: evidence for rolling-circle replication in a hyperthermophile (1996)

The plasmid pGT5 (3,444 bp) from the hyperthermophilic archaeon Pyrococcus abyssi GE5 has been completely sequenced. Two major open reading frames with a good coding probability are located on the same strand and cover 85% of the total sequence. The larger open reading frame encodes a putative polypeptide which exhibits sequence similarity with Rep proteins of plasmids using the rolling-circle mechanism for replication. Upstream of this open reading frame, we have detected an 11-bp motif identical to the double-stranded origin of several bacterial plasmids that replicate via the rolling-circle mechanism. A putative single-stranded origin exhibits similarities both to bacterial primosome-dependent single-stranded initiation sites and to bacterial primase (dnaG) start sites. A single-stranded form of pGT5 corresponding to the plus strand was detected in cells of P. abyssi. These data indicate that pGT5 replicates via the rolling-circle mechanism and suggest that members of the domain Archaea contain homologs of several bacterial proteins involved in chromosomal DNA replication. Phylogenetic analysis of Rep proteins from rolling-circle replicons suggest that diverse families diverged before the separation of the domains Archaea, Bacteria, and Eucarya.
The interrogation of a conventional current transformer using an in-fibre Bragg grating (1997)

An alternative approach for measuring large currents at high potentials is presented. The output signal from a conventional current transformer is measured by transducing the voltage developed across the transformer secondary into a shift in the reflected wavelength from an in-fibre Bragg grating using a piezoelectric element. A pseudo-heterodyne detection scheme was used to determine this shift. We demonstrate the scheme's performance over a current range of 700 A. The current resolution was about 1.5 A HZ(-1/2) over a frequency range of about 50 to 5 x 10(3) Hz.
On content-centric router design and implications (2010)

In this paper, we investigate a sample line-speed content-centric router's design, its resources and its usage scenarios. We specifically take a closer look at one of the suggested functionalities for these routers, the content store. The design is targeted at pull-based environments, where content can be pulled from the network by any interested entity. We discuss the interaction between the pull-based protocols and the content-centric router. We also provide some basic feasibility metrics, discussing some applicability aspects for such routers.
Intranasal Apomorphine in Parkinsonian On-Off Fluctuations (1992)

The efficacy of intranasal apomorphine was assessed in seven patients with Parkinson's disease and severe levodopa (L-dopa)-related "off-period" disabilities. All patients responded favorably to treatment with intranasal apomorphine. The speed and the quality of motor response and the pharmacokinetic profile showed results similar to those seen after subcutaneous injection of apomorphine administered by insulin pen syringe. The simplicity in the technique of intranasal apomorphine administration was found to be superior by all patients.
Macular zeaxanthins and lutein – a review of dietary sources and bioavailability and some relationships with macular pigment optical density and age-related macular disease (2007)

The retina is unique in the human body in containing three xanthophyll carotenoids; 3R,3'R-zeaxanthin, meso-zeaxanthin (MZ) and lutein. Humans consume 1 to 3 mg lutein per d and the lutein:zeaxanthin ratio in the diet is about 5:1.Xanthophyll pigments occur widely in vegetables and fruits but MZ is found in only a few foods such as the shrimp carapace and fish skin. In spite of the amounts of the different xanthophylls in the diet, zeaxanthin and MZ occur in approximately equal amounts in the eye, and their combined concentration can exceed that of lutein. In the present review the bioavailablity of zeaxanthin and lutein is assessed using the plasma xanthophyll response to dietary intervention. A number of studies have used single and mixed sources of the pure xanthophylls to achieve steady-state plasma responses. Mostly these have been with lutein and zeaxanthin but two using MZ are also described. Responses following the intervention with the pure xanthophylls are compared with those following food intervention. Vegetables are the richest source of dietary lutein and several vegetable-feeding studies are discussed. Intervention studies with eggs, which are a good source of zeaxanthin, suggest that the xanthophyll carotenoids in egg yolk may be more bioavailable than those in other foods and are described separately. MZ has been a component of a xanthophyll supplement added to chicken feed in Mexico in the last 10 years. Egg consumption in Mexico is approximately one egg/person per d and the potential contribution of this food source of MZ to Mexican dietary intakes is described. Very limited information from human feeding studies of MZ-containing supplements suggests that MZ is less well absorbed than zeaxanthin. However, MZ is unusual in the diet and not reported in the plasma. Thus plasma responses may not reflect true absorption if it takes MZ longer to equilibrate with body tissues than the other xanthophylls and competition with zeaxanthin may lower the relative concentrations of MZ in plasma. Lastly, the effects of long-term feeding with both pure and food sources of the xanthophyll pigments on macular pigment optical density is compared and the importance of previous dietary intake on the effects of intervention is discussed.
Hydroxylation by cytochrome P-450 and metalloporphyrin models. Evidence for allylic rearrangement (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydroxylation by cytochrome P-450 and metalloporphyrin models. Evidence for allylic rearrangementJohn T. Groves and Durga V. AdhyamCite this: J. Am. Chem. Soc. 1984, 106, 7, 2177–2181Publication Date (Print):April 1, 1984Publication History Published online1 May 2002Published inissue 1 April 1984https://pubs.acs.org/doi/10.1021/ja00319a044https://doi.org/10.1021/ja00319a044research-articleACS PublicationsRequest reuse permissionsArticle Views895Altmetric-Citations193LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Selecting Key Poses on Manifold for Pairwise Action Recognition (2011)

In action recognition, bag of visual words based approaches have been shown to be successful, for which the quality of codebook is critical. In a large vocabulary of poses (visual words), some key poses play a more decisive role than others in the codebook. This paper proposes a novel approach for key poses selection, which models the descriptor space utilizing a manifold learning technique to recover the geometric structure of the descriptors on a lower dimensional manifold. A PageRank-based centrality measure is developed to select key poses according to the recovered geometric structure. In each step, a key pose is selected from the manifold and the remaining model is modified to maximize the discriminative power of selected codebook. With the obtained codebook, each action can be represented with a histogram of the key poses. To solve the ambiguity between some action classes, a pairwise subdivision is executed to select discriminative codebooks for further recognition. Experiments on benchmark datasets showed that our method is able to obtain better performance compared with other state-of-the-art methods.
Enhanced NMDA Receptor-Mediated Synaptic Transmission, Enhanced Long-Term Potentiation, and Impaired Learning and Memory in Mice Lacking IRSp53 (2009)

IRSp53 is an adaptor protein that acts downstream of Rac and Cdc42 small GTPases and is implicated in the regulation of membrane deformation and actin filament assembly. In neurons, IRSp53 is an abundant postsynaptic protein and regulates actin-rich dendritic spines; however, its in vivo functions have not been explored. We characterized transgenic mice deficient of IRSp53 expression. Unexpectedly, IRSp53(-/-) neurons do not show significant changes in the density and ultrastructural morphologies of dendritic spines. Instead, IRSp53(-/-) neurons exhibit reduced AMPA/NMDA ratio of excitatory synaptic transmission and a selective increase in NMDA but not AMPA receptor-mediated transmission. IRSp53(-/-) hippocampal slices show a markedly enhanced long-term potentiation (LTP) with no changes in long-term depression. LTP-inducing theta burst stimulation enhances NMDA receptor-mediated transmission. Spatial learning and novel object recognition are impaired in IRSp53(-/-) mice. These results suggest that IRSp53 is involved in the regulation of NMDA receptor-mediated excitatory synaptic transmission, LTP, and learning and memory behaviors.
Possible mechanisms of muscle cramp from temporal and spatial surface EMG characteristics (2000)

In this study, the initiation and development of muscle cramp are investigated. For this, we used a 64-channel surface electromyogram (EMG) to study the triceps surae muscle during both cramp and maximal voluntary contraction (MVC) in four cramp-prone subjects and during cramp only in another four cramp-prone subjects. The results show that cramp presents itself as a contraction of a slowly moving fraction of muscle fibers, indicating that either the spatial arrangement of the motoneurons and muscle fibers is highly related or that cramp spreads at a level close to the muscle. Spectral analyses of the EMG and peak-triggered average potentials show the presence of extremely short potentials during cramp compared with during MVC. These results can also be interpreted in two ways. Either the motoneurons fire with enlarged synchronization during MVC compared with cramp, or smaller units than motor units are active, indicating that cramp is initiated close to or even at the muscle fiber level. Further research is needed to draw final conclusions.
Líquen plano oral (2011)

Oral lichen planus (OLP) is a relatively common mucosal disease that can present isolated or associated with cutaneous lichen planus. Contrarily to its cutaneous counterpart, though, OLP tends to be chronic, relapsing, and difficult to treat. Severe morbidity is related to erosive forms, and more aggressive presentations have been described, such as the "gingivo-vulvar syndrome". This article reviews the current knowledge about the pathogenesis, clinical picture, differential and laboratorial diagnosis, prognosis, and treatment of OLP.
Differential effects of protein kinase C inhibitors on chemokine production in human synovial fibroblasts (1996)

1. Rheumatoid arthritis is associated with the accumulation and activation of selected populations of inflammatory cells within the arthritic joint. One putative signal for this process is the production, by resident cells, of a group of inflammatory mediators known as the chemokines. 2. The chemokines interleukin-8 (IL-8), monocyte chemotactic protein-1 (MCP-1) and RANTES (regulated on activation normal T-cell expressed and presumably secreted) are target-cell specific chemoattractants produced by synovial fibroblasts in response to stimulation with interleukin-1 alpha (IL-1 alpha) or tumour necrosis factor alpha (TNF alpha). The signalling pathways involved in their production are not well defined. We therefore used four different protein kinase C inhibitors to investigate the role of this kinase in the regulation of chemokine mRNA and protein expression in human cultured synovial fibroblasts. 3. The non-selective PKC inhibitor, staurosporine (1-300 nM) significantly increased the production of IL-1 alpha-induced IL-8 mRNA and protein. A specific PKC inhibitor, chelerythrine chloride (0.1-3 microM), also caused a small concentration-dependent increase in IL-8 mRNA and protein production. In contrast, 3-[1-[3-(amidinothio)propyl]-3-indoly]-4-(1-methyl-3-indolyl )- 1H-pyrrole-2,5-dione methanesulphonate (Ro 31-8220) and 2[1-(3-dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3- yl)-maleimide (GF 109203X), two selective PKC inhibitors of the substituted bisindolylmaleimide family had a concentration-dependent biphasic effect on IL-1 alpha or TNF alpha-induced chemokine expression. At low concentrations they caused a stimulation in chemokine production, which was especially evident at the mRNA level. At higher concentrations both inhibited IL-1 alpha or TNF alpha-induced chemokine mRNA and protein production. Ro 31-8220 was 10 fold more potent than GF 109203X, with an IC50 of 1.6 +/- 0.08 microM (mean +/- s.e.mean, n = 4) for IL-1 alpha induced IL-8 production. Ro 31-8220 also inhibited the expression of IL-1 alpha or TNF alpha-induced MCP-1 and RANTES mRNA with a similar potency. 4. The stimulatory effect of staurosporine is discussed in relation to the known poor selectivity of this inhibitor for PKC. It is proposed that activation of an isoform of PKC, possibly PKC epsilon or zeta, which is inhibited by higher concentrations of the bisinodolylmaleimides, plays a role in the regulation of chemokine expression induced by IL-1 alpha or TNF alpha in synovial cells. 5. The inhibition of chemokine production by bisindolylmaleimide compounds heralds a novel approach for future anti-inflammatory therapies.
Simulation of electromagnetic well‐logging tools by the Nédélec edge finite elements (2007)

The work is devoted to 3D‐numerical simulation of electromagnetic resistivity logging tools in the frequency domain, using the finite element method with Nádélec edge basic functions on a cylindrical grid. It considers the discretization method, properties of the arising discrete problems, and proposes an algebraic solver for them. Results of practical application are also presented.
Model cell culture system for defining the molecular and biochemical events mediating terminal differentiation of human melanoma cells (2008)

Abstract Cancer cells are commonly less differentiated than their normal progenitors; a phenotype that correlates with loss of specialized functions and an increased capability to self‐renew. Melanoma is an ideal model to analyze cancer progression and differentiation since a well‐characterized process of step‐wise tumor progression has been defined. Our lab previously described a combinatorial in vitro treatment protocol to induce terminal differentiation of human melanoma cells using a low dose of the PKC activator Mezerein (Mez) combined with interferon‐β (IFN‐β), which also activates IFN‐stimulated gene expression in addition to the re‐differentiation program. In principle, using an alternate way to induce terminal differentiation not including IFN‐β would be more compatible with gene expression profiling. A higher concentration of Mez alone induced terminal differentiation of HO‐1 human melanoma cells as measured by morphological, growth and biochemical assays. Pre‐treatment with the PKC inhibitor GF109203x blocked changes associated with differentiation and inhibited the ability of Mez to force irreversible/terminal differentiation. By combining this efficient method of inducing terminal differentiation with microarray analyses we now identify potential regulators of this process and demonstrate utility of this novel in vitro model in which to study the molecular determinants and mechanisms of human melanoma differentiation. J. Cell. Physiol. 218: 304–314, 2009. © 2008 Wiley‐Liss, Inc.
Reed-Sternberg cell: survival in a hostile sea. (1998)

In contrast to the non-Hodgkin's lymphomas, little is known regarding the origin, genetics, and function of the Reed-Sternberg cell of Hodgkin's disease. Unlike other cancers, the neoplastic cell of Hodgkin's disease, the Reed-Sternberg cell, is vastly outnumbered by a surrounding intense inflammatory infiltrate. How this rare neoplastic cell originates, persists, and disseminates in a presumably hostile cellular environment has remained a mystery. Understanding the biology of the Reed-Sternberg cell has been impeded by the rarity of the cell in tumor tissue. Herein, we describe how the application of single-cell genetic analysis has revealed a clonal and, possibly, germinal center B-cell origin of the Reed-Sternberg cell. By phenotype and function, Reed-Sternberg cells are highly interactive with their cellular microenvironment through cell-cell adhesion, expression of members of the tumor necrosis factor receptor superfamily, and elaboration of cytokines. Perhaps by their mimicry of immune system cells with antigen-presenting function, Reed-Sternberg cells mediate the unusual clinical and pathologic features of Hodgkin's disease: intense tissue inflammatory infiltrate, fibrosis, and constitutional symptoms.
Optimal Alarm Signal Processing: Filter Design and Performance Analysis (2013)

Accuracy and efficiency of alarm systems are of paramount importance in safe operations of industrial processes. Accuracy is measured by false and missed alarm rates (probabilities); while efficiency relates to the detection delay and complexity of the technique used. Moving average filters are often employed in industry for improved alarm accuracy. Can one do better than moving average filters? The following two problems are studied in this paper: First, given both normal and abnormal statistic distributions, how to design an optimal alarm filter (of fixed complexity) for best alarm accuracy, minimizing a weighted sum of false and missed alarm rates? Second, in what cases are moving average filters optimal? For the first problem, design of optimal linear FIR alarm filters is studied, and a numerical optimization based procedure is proposed. For the second problem, a sufficient condition is given under which the moving average filters are optimal.
Multimedia-IT-tools for global cooperation in engineering networks (2002)

To expanse the share of the global markets, manufacturers of capital goods have to secure a high customer closeness. Customer closeness means not only a quick service support if problems occur. The rising complexity of capitol goods and technological processes forces the efficient support of the manufacturer along the whole product life cycle. Global markets also call for customer closeness over long geographical distances between customer, manufacturer and supplier. With conventional tools, one can only fit cost intensive demands with a number of restrictions. Efficient internal organisation structures and external enterprise spread engineering networks combined with modern information and communication-tools can provide solutions for high customer closeness over long distances.