gebhart/patrepeval huggingface dataset:
Molecular cloning and biochemical characterization of a lipoxygenase in almond (<i>Prunus dulcis</i>) seed (2001)

We have characterized an almond (Prunus dulcis) lipoxygenase (LOX) that is expressed early in seed development. The presence of an active lipoxygenase was confirmed by western blot analysis and by measuring the enzymatic activity in microsomal and soluble protein samples purified from almond seeds at this stage of development. The almond lipoxygenase, which had a pH optimum around 6, was identified as a 9-LOX on the basis of the isomers of linoleic acid hydroperoxides produced in the enzymatic reaction. A genomic clone containing a complete lipoxygenase gene was isolated from an almond DNA library. The 6776-bp sequence reported includes an open reading frame of 4667 bp encoding a putative polypeptide of 862 amino acids with a calculated molecular mass of 98.0 kDa and a predicted pI of 5.61. Almond seed lipoxygenase shows 71% identity with an Arabidopsis LOX1 gene and is closely related to tomato fruit and potato tuber lipoxygenases. The sequence of the active site was consistent with the isolated gene encoding a 9-LOX.
[Expression pattern of profilin homologue in embryogenesis of amphioxus]. (2004)

The expression pattern of profilin homologue in embryogenesis of amphioxus was characterized by whole-mount in situ hybridization combined serial histological sections and Northern blot. The results revealed that the dynamic expression pattern of the profilin homologue just accorded with muscle differentiating in this animal. It was most possibly involved in the development of muscle in amphioxus. The result of Southern blot supported that there was only one copy of profilin homologue in the genome of Qingdao amphioxus. It may be good for explaining the molecular mechanism of muscle differentiation in amphioxus.
Clinical Evaluation of Respiratory Motion Compensation for Anatomical Roadmap Guided Cardiac Electrophysiology Procedures (2011)

X-ray fluoroscopically guided cardiac electrophysiological procedures are routinely carried out for diagnosis and treatment of cardiac arrhythmias. X-ray images have poor soft tissue contrast and, for this reason, overlay of static 3-D roadmaps derived from preprocedural volumetric data can be used to add anatomical information. However, the registration between the 3-D roadmap and the 2-D X-ray image can be compromised by patient respiratory motion. Three methods were designed and evaluated to correct for respiratory motion using features in the 2-D X-ray images. The first method is based on tracking either the diaphragm or the heart border using the image intensity in a region of interest. The second method detects the tracheal bifurcation using the generalized Hough transform and a 3-D model derived from 3-D preoperative volumetric data. The third method is based on tracking the coronary sinus (CS) catheter. This method uses blob detection to find all possible catheter electrodes in the X-ray image. A cost function is applied to select one CS catheter from all catheter-like objects. All three methods were applied to X-ray images from 18 patients undergoing radiofrequency ablation for the treatment of atrial fibrillation. The 2-D target registration errors (TRE) at the pulmonary veins were calculated to validate the methods. A TRE of 1.6 mm ± 0.8 mm was achieved for the diaphragm tracking; 1.7 mm ± 0.9 mm for heart border tracking, 1.9 mm ± 1.0 mm for trachea tracking, and 1.8 mm ± 0.9 mm for CS catheter tracking. We present a comprehensive comparison between the techniques in terms of robustness, as computed by tracking errors, and accuracy, as computed by TRE using two independent approaches.
Dual‐opposite‐injection CZE: Theoretical aspects and application to organic and pharmaceutical compounds (2007)

Several important figures of merit (migration time, efficiency, resolution, resolution per unit time, and electrophoretic selectivity) are quantitatively compared for the first time for conventional CZE and dual-opposite-injection CZE (DOI-CZE). Aspects of DOI-CZE relevant to the separation of organic and pharmaceutical ions (MW>120 Da) are also discussed. Two new approaches to resolve the codetection of anions and cations, hydrodynamic flow-modified DOI-CZE and polarity reversal in combination with asymmetric detector window positioning, are compared with the method of preliminary transport, a variable procedure within sequential sample introduction, using a six-component sample of organic and pharmaceutical compounds. The advantages of DOI-CZE for the simultaneous analysis of organic/pharmaceutical anions and cations are illustrated in a direct comparison of conventional CZE and DOI-CZE for the separation of a ten-component mixture of pharmaceutical ions (five ionized acids and five ionized bases).
AB-Stacked Multilayer Graphene Synthesized <i>via</i> Chemical Vapor Deposition: A Characterization by Hot Carrier Transport (2012)

We report the synthesis of AB-stacked multilayer graphene via ambient pressure chemical vapor deposition on Cu foils and demonstrate a method to construct suspended multilayer graphene devices. In four-terminal geometry, such devices were characterized by hot carrier transport at temperatures down to 240 mK and in magnetic fields up to 14 T. The differential conductance (dI/dV) shows a characteristic dip at longitudinal voltage bias V = 0 at low temperatures, indicating the presence of hot electron effect due to a weak electron-phonon coupling. Under magnetic fields, the magnitude of the dI/dV dip diminishes through the enhanced intra-Landau level cyclotron phonon scattering. Our results provide new perspectives in obtaining and understanding AB-stacked multilayer graphene, important for future graphene-based applications.
The activation and maintenance of<i>Pax2</i>expression at the mid-hindbrain boundary is controlled by separate enhancers (2002)

Pax2 is the earliest known gene to be expressed throughout the mid-hindbrain region in late gastrula embryos of the mouse and is essential for the formation of an organizing center at the midbrain-hindbrain boundary (MHB), which controls midbrain and cerebellum development. We have used transgenic analysis to identify three MHB-specific enhancers in the upstream region of the mouse Pax2 gene. A 120 bp enhancer (at -3.7 kb) in cooperation with the endogenous promoter was sufficient to induce transgene expression in the anterior neural plate of late gastrula embryos, while it was already inactivated again at the MHB during somitogenesis. The activity of this early enhancer was severely reduced by mutation of three homeodomain-binding sites, two of which are part of a recognition sequence for POU homeodomain proteins. Oct3/4 (Pou5f1), the mouse ortholog of zebrafish Pou2, efficiently bound to this sequence, suggesting its involvement in the regulation of the early Pax2 enhancer. Starting at the four-somite stage, Pax2 is expressed at the MHB under the control of two enhancers located at -4.1 kb and -2.8 kb. The distal late enhancer contains a 102 bp sequence that is not only highly conserved between the mouse and pufferfish Pax2 genes, but also contributes to the enhancer activity of both genes in transgenic mice. The proximal 410 bp enhancer, which overlaps with a kidney-specific regulatory element, contains a functional Pax2/5/8-binding site and thus maintains Pax2 expression at the MHB under auto- and cross-regulatory control by Pax2/5/8 proteins. Importantly, the early and proximal late enhancers are not only sufficient but also necessary for expression at the MHB in the genomic context of the Pax2 locus, as their specific deletion interfered with correct temporal expression of a large Pax2 BAC transgene. Hence, separate enhancers under the control of distinct transcription factors activate and maintain Pax2 expression at the MHB.
Automatic and Noninvasive Indoor Air Quality Control in HVAC Systems (2016)

This paper presents a methodology for assessing and monitoring the cleaning state of a heating, ventilation, and air conditioning (HVAC) system of a building. It consists of a noninvasive method for measuring the amount of dust in the whole ventilation system, that is, the set of filters and air ducts. Specifically, it defines the minimum amount of measurements, their time table, locations, and acquisition conditions. The proposed method promotes early intervention on the system and it guarantees high indoor air quality and proper HVAC working conditions. The effectiveness of the method is proved by some experimental results on different study cases.
SUMMARY OF PERIODICAL LITERATURE (1964)

SUMMARY OF PERIODICAL LITERATURE EDWIN MORGAN, EDWIN MORGAN UNIVERSITY OF GLASGOW Search for other works by this author on: Oxford Academic Google Scholar JOHN A. M. RILLIE JOHN A. M. RILLIE UNIVERSITY OF GLASGOW Search for other works by this author on: Oxford Academic Google Scholar The Review of English Studies, Volume XV, Issue 57, 1964, Pages 120–123, https://doi.org/10.1093/res/XV.57.120 Published: 01 January 1964
THE EFFECTS OF ISOSTEVIOL AGAINST MYOCARDIUM INJURY INDUCED BY ISCHAEMIA–REPERFUSION IN THE ISOLATED GUINEA PIG HEART (2007)

1. This study aimed to investigate the protective effects of isosteviol against myocardial ischaemia-reperfusion (IR) injury and its effects on mitochondrial adenosine triphosphate (ATP)-sensitive potassium channel (mitoK(ATP)) activity in vitro. 2. Groups of eight guinea pigs were treated as follows: constant perfusion control (PC), IR control, ischaemic preconditioning (IPC) + IR, isosteviol (50, 250 or 500 nmol) + IR, 5-hydroxydecanoate acid (5-HD) (5 micromol) + isosteviol (500 nmol) + IR. The guinea pig heart was isolated and perfused in Langendorff mode with modified Tyrode solution at a flow rate of 10 mL/min. Ischaemia was introduced for 30 min followed by reperfusion for 20 min. Cardiac function, coronary arterial flow rate, lactate dehydrogenase (LDH) and creatine kinase (CK) activities in the perfusate were measured prior to ischaemia and at the end of reperfusion. 3. There were no significant (P > 0.05) changes in cardiac function or markers of cell damage (i.e. activities of LDH and CK) in the PC group. In contrast, cardiac function was adversely affected in the IR group, with significant (P < 0.05) decreases in left ventricular developing pressure (LVDevP), dP/dt(max) and dP/dt(min) compared with baseline and the PC group. In addition, there were increases in activity of LDH (20%) and CK (67%) compared with baseline and the PC group. 4. Ischaemic preconditioning and pretreatment with isosteviol, at all dose levels, resulted in a significant (P < 0.05) attenuation of IR injury. Lactate dehydrogenase and CK activities were not significantly (P < 0.05) different compared with baseline. Isosteviol did not increase coronary flow, suggesting that the protective effect of isosteviol on the myocardium was not mediated by dilation of the coronary blood vessels. 5. Pretreatment with the mitoK(ATP) blocker 5-HD partially antagonized the effects of 500 nmol isosteviol, with a statistically significant attenuation of its protective effects on HR, LVDevP, dP/dt(max) and dP/dt(min) compared with isosteviol alone pretreatment. 6. The IR injury on the Langendorff perfused guinea pig heart was alleviated by isosteviol, which appears to mediate its effects through mitoK(ATP) channels. Future research might aim to investigate the interaction of isosteviol with mitoK(ATP) channels in order to clarify its mechanism of action.
Getting Chemicals into Trees Without Spraying (2011)

This fact sheet provides an overview of injection, implantation, and other ways to get chemicals, mainly pesticides, into trees. Many techniques and systems exist and some are very good, some are good in some situations, and some are ineffective or bad for trees. This fact sheet addresses all of these.
L-ergothioneine level in red blood cells of healthy human males in the Western province of Saudi Arabia (2001)

Ergothioneine is widely distributed in biological systems, particularly in red blood cells of animals. However, it's functional role in human body is not well understood. In order to investigate the biochemical effect of L-ergothioneine, its concentration changes in human blood with respect to ages in healthy individuals was first investigated. L-ergothioneine concentrations in the blood of Saudi males from western province at different stages of life were measured by the procedure of Carlsson et al., 1974. At early stages of life (1-10 years), the concentrations of LER is 1.5-2.0 mg/100 ml. It increases gradually at the age of 11-18 years where it reaches the maximum value of 3.7 mg/100 ml. Then, it declines gradually to 3.0-2.3 mg/ 100 ml during the period of 19-50 years. An increase in the level of LER (2.8 mg/100 ml) was seen at the age of 51+.
Robust Timely Scheduled Optical Burst Switching (2007)

The two-way reservation based optical-burst-switching scheme is implemented over existing distributed-control optical-circuit-switching networks through the novel robust timely scheduled reservation protocol to guarantee data delivery and minimize burst blocking while maximizing wavelength utilization.
The effect of vitamin E on the structure of membrane lipid assemblies (2003)

The effects of vitamin E on the activity of membrane-dependent enzymes suggest that it acts indirectly by modifying some properties of the lipid host. The effects of alpha-tocopherol (alpha-T) and alpha-tocopherol hemisuccinate (alpha-THS) on phospholipid monolayer structure, curvature, and bending elasticity were examined using X-ray diffraction and the osmotic stress method. These ligands were mixed with the hexagonal phase-forming lipid, dioleoylphosphatidylethanolamine (DOPE). Increasing levels up to 50 mol% alpha-T in DOPE in excess water result in a systematic decrease in the lattice dimension. Analysis of the structural changes imposed by alpha-T shows that it contributes a spontaneous radius of curvature of -13.7 A. This unusually negative value is comparable to diacylglycerols. alpha-T does not affect the bending elasticity of these monolayers. alpha-THS in its charged form decreases membrane curvature, but in its undissociated neutral form has a qualitatively similar but reduced effect on monolayer curvature, as does alpha-T. We discuss these results in terms of the local stresses such ligands would produce in the vicinity of a membrane protein, and how one might expect proteins to respond to such stress.
A Query Language for Multimedia Content (2007)

The growing amount of digital multimedia data available today and the de-facto MPEG-7 standard for multimedia content description has lead to the requirement of a query language for multimedia content. MPEG-7 is expressed in XML and it defines descriptors of the multimedia content such as audio-visual descriptors, location and time attributes as well as other metadata such as media author, media Uri and more. While most search solutions for multimedia today are based on text annotations, having the MPEG-7 standard opens an opportunity for real multimedia content based retrieval. In this paper we propose an IR-style query language for such multimedia content based retrieval that exploits the XML representation of MPEG-7. The query language is an extension of the “XML Fragments ” query language that was originally designed as a Query-By-Example for text-only XML collections. We mainly focus on the unique characteristics of Multimedia content which needs to support similarity search query (range search and K-nearest neighbors) and queries on spatio-temporal attributes.
The prognostic value of predischarge quantitative two-dimensional echocardiographic measurements and the effects of early lisinopril treatment on left ventricular structure and function after acute myocardial infarction in the GISSI-3 Trial (1996)

2-D echocardiography independently contributes to pre-discharge risk stratification in terms of 6-month mortality and clinical heart failure after myocardial infarction, and early, short-term treatment with lisinopril in unselected myocardial infarction patients attenuates left ventricular dilatation; an effect evident in patients with larger infarcts. These results probably only partly explain the effect of lisinopril on total mortality concentrated in the first week after infarction.
Field Application of HCl-Formic Acid System to Acid Fracture Deep Gas Wells Completed with Super Cr-13 Tubing in Saudi Arabia (2003)

Abstract This paper describes optimization of gelled and in-situ gelled HCl/formic acid systems to stimulate deep gas wells completed with super Cr-13 tubing. High temperatures encountered in deep wells and the susceptibility of super Cr-13 to severe corrosion by concentrated HCl solutions and high chloride ion concentrations render acid stimulation a very difficult task. Therefore, extensive experimental and field studies were performed to develop a cost effective acid system to enhance the productivity of deep gas wells, while maintaining the integrity of the super Cr-13 tubing. Coreflood tests indicated that this acid system can create deep wormholes in tight reservoir cores (less than 1 md). Corrosion tests, however, indicated that protecting super Cr-13 tubulars during pumping this acid (15 wt% HCl/9 wt% formic acid) is not an easy task, especially at downhole temperatures (275°F). A very limited number of inhibitor aids were found to be effective in protecting super Cr-13 at reservoir conditions. To optimize the corrosion inhibitor package, the bottom hole temperature was measured in a few wells during acid injection. Based on lab tests and field measurements, an acid treatment was designed and applied in the field. The treated wells responded positively to the treatment. Most importantly, the integrity of the super Cr-13 was maintained. This was confirmed from chemical analysis of well flowback samples following the treatment and measurement of the tubing inside diameter before and after the treatment. The maximum Cr concentration in the flowback sample was less than 2 mg/L. Molybdenum and nickel concentrations were less than 1 mg/L. The total iron concentration was less than 200 mg/L. This value is significantly less than that noted with wells completed with low-carbon steel tubulars.
The ferroelectric phases derived from the 4-<i>n</i>-alkoxycinnamic acids (1984)

Abstract The optically active 4-(2-methylbutyloxy)phenol and 4-hydroxy-(2′-methylbutyl) benzoate esters of the p-n-alkoxycinnamic acids have been made and characterized. These materials show similar ferroelectric liquid crystal properties to and in a temperature range between those found for the analogous two and three benzene ring systems. These materials provide chiral smectic C phases in intermediate temperatures ranges (50-90°C) required to produce eutectic ferroelectric mixtures over a wide range of temperatures.
Novel Type of Specialized Transduction forCTXφ or Its Satellite Phage RS1 Mediated by Filamentous PhageVGJφ in <i>Vibriocholerae</i> (2003)

The main virulence factor of Vibrio cholerae, the cholera toxin, is encoded by the ctxAB operon, which is contained in the genome of the lysogenic filamentous phage CTX phi. This phage transmits ctxAB genes between V. cholerae bacterial populations that express toxin-coregulated pilus (TCP), the CTX phi receptor. In investigating new forms of ctxAB transmission, we found that V. cholerae filamentous phage VGJ phi, which uses the mannose-sensitive hemagglutinin (MSHA) pilus as a receptor, transmits CTX phi or its satellite phage RS1 by an efficient and highly specific TCP-independent mechanism. This is a novel type of specialized transduction consisting in the site-specific cointegration of VGJ phi and CTX phi (or RS1) replicative forms to produce a single hybrid molecule, which generates a single-stranded DNA hybrid genome that is packaged into hybrid viral particles designated HybP phi (for the VGJ phi/CTX phi hybrid) and HybRS phi (for the VGJ phi/RS1 hybrid). The hybrid phages replicate by using the VGJ phi replicating functions and use the VGJ phi capsid, retaining the ability to infect via MSHA. The hybrid phages infect most tested strains more efficiently than CTX phi, even under in vitro optimal conditions for TCP expression. Infection and lysogenization with HybP phi revert the V. cholerae live attenuated vaccine strain 1333 to virulence. Our results reinforce that TCP is not indispensable for the acquisition of CTX phi. Thus, we discuss an alternative to the current accepted evolutionary model for the emergence of new toxigenic strains of V. cholerae and the importance of our findings for the development of an environmentally safer live attenuated cholera vaccine.
2.4 GHz Radio Transmission Measurements in a Basin filled with Sea Water (2013)

International audience
Naturally Occurring Lipid A Mutants in Neisseria meningitidis from Patients with Invasive Meningococcal Disease Are Associated with Reduced Coagulopathy (2009)

Neisseria meningitidis is a major cause of bacterial meningitis and sepsis worldwide. Lipopolysaccharide (LPS), a major component of the Gram-negative bacterial outer membrane, is sensed by mammalian cells through Toll-like receptor 4 (TLR4), resulting in activation of proinflammatory cytokine pathways. TLR4 recognizes the lipid A moiety of the LPS molecule, and the chemical composition of the lipid A determines how well it is recognized by TLR4. N. meningitidis has been reported to produce lipid A with six acyl chains, the optimal number for TLR4 recognition. Indeed, meningococcal sepsis is generally seen as the prototypical endotoxin-mediated disease. In the present study, we screened meningococcal disease isolates from 464 patients for their ability to induce cytokine production in vitro. We found that around 9% of them were dramatically less potent than wild-type strains. Analysis of the lipid A of several of the low-activity strains by mass spectrometry revealed they were penta-acylated, suggesting a mutation in the lpxL1 or lpxL2 genes required for addition of secondary acyl chains. Sequencing of these genes showed that all the low activity strains had mutations that inactivated the lpxL1 gene. In order to see whether lpxL1 mutants might give a different clinical picture, we investigated the clinical correlate of these mutations in a prospective nationwide observational cohort study of adults with meningococcal meningitis. Patients infected with an lpxL1 mutant presented significantly less frequently with rash and had higher thrombocyte counts, consistent with reduced cytokine induction and less activation of tissue-factor mediated coagulopathy. In conclusion, here we report for the first time that a surprisingly large fraction of meningococcal clinical isolates have LPS with underacylated lipid A due to mutations in the lpxL1 gene. The resulting low-activity LPS may have an important role in virulence by aiding the bacteria to evade the innate immune system. Our results provide the first example of a specific mutation in N. meningitidis that can be correlated with the clinical course of meningococcal disease.
Date and Time on the Internet: Timestamps (2002)

This document defines a date and time format for use in Internet protocols that is a profile of the ISO 8601 standard for representation of dates and times using the Gregorian calendar.