gebhart/patrepeval huggingface dataset:
Signals for the incorporation and orientation of cytochrome P450 in the endoplasmic reticulum membrane. (1988)

Cytochrome P450b is an integral membrane protein of the rat hepatocyte endoplasmic reticulum (ER) which is cotranslationally inserted into the membrane but remains largely exposed on its cytoplasmic surface. The extreme hydrophobicity of the amino-terminal portion of P450b suggests that it not only serves to initiate the cotranslational insertion of the nascent polypeptide but that it also halts translocation of downstream portions into the lumen of the ER and anchors the mature protein in the membrane. In an in vitro system, we studied the cotranslational insertion into ER membranes of the normal P450b polypeptide and of various deletion variants and chimeric proteins that contain portion of P450b linked to segments of pregrowth hormone or bovine opsin. The results directly established that the amino-terminal 20 residues of P450b function as a combined insertion-halt-transfer signal. Evidence was also obtained that suggests that during the early stages of insertion, this signal enters the membrane in a loop configuration since, when the amino-terminal hydrophobic segment was placed immediately before a signal peptide cleavage site, cleavage by the luminally located signal peptidase took place. After entering the membrane, the P450b signal, however, appeared to be capable of reorienting within the membrane since a bovine opsin peptide segment linked to the amino terminus of the signal became translocated into the microsomal lumen. It was also found that, in addition to the amino-terminal combined insertion-halt-transfer signal, only one other segment within the P450b polypeptide, located between residues 167 and 185, could serve as a halt-transfer signal and membrane-anchoring domain. This segment was shown to prevent translocation of downstream sequences when the amino-terminal combined signal was replaced by the conventional cleavable insertion signal of a secretory protein.
Methylcitrate synthase from <i>Aspergillus nidulans</i>: implications for propionate as an antifungal agent (2000)

Aspergillus nidulans was used as a model organism to investigate the fungal propionate metabolism and the mechanism of growth inhibition by propionate. The fungus is able to grow slowly on propionate as sole carbon and energy source. Propionate is oxidized to pyruvate via the methylcitrate cycle. The key enzyme methylcitrate synthase was purified and the corresponding gene mcsA, which contains two introns, was cloned, sequenced and overexpressed in A. nidulans. The derived amino acid sequence of the enzyme shows more than 50% identity to those of most eukaryotic citrate synthases, but only 14% identity to the sequence of the recently detected bacterial methylcitrate synthase from Escherichia coli. A mcsA deletion strain was unable to grow on propionate. The inhibitory growth effect of propionate on glucose medium was enhanced in this strain, which led to the assumption that trapping of the available CoA as propionyl-CoA and/or the accumulating propionyl-CoA itself interferes with other biosynthetic pathways such as fatty acid and polyketide syntheses. In the wild-type strain, however, the predominant inhibitor may be methylcitrate. Propionate (100 mM) not only impaired hyphal growth of A. nidulans but also synthesis of the green polyketide-derived pigment of the conidia, whereas in the mutant pigmentation was abolished with 20 mM propionate.
A 45-Mbits/sec. VLSI Viterbi decoder for digital video applications (2002)

The author describes the unique architecture and feature set incorporated into the STEL-2060 Viterbi Decoder, a VLSI device designed by Standford Telecom. This device was designed primarily for direct broadcasting by satellite (DBS) services and operates up to 45 Mb/s. The STEL-2060 uses industry standard, constraint length 7, polynomials and incorporates depuncturing circuitry to allow it to be used with punctured codes up to rate 7/8, as well as standard rate 1/2 coding.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Resistance heating using electrically conductive cements (2004)

Steel fibre (8 μm diameter, 0·72 vol.%)-reinforced cement was found to be effective for resistance heating. A DC electrical power input of 5·6 W (7·1 V, 0·79 A) resulted in a maximum temperature of 60°C (initial temperature = 19°C) and a time of 6 min to reach half the maximum temperature. Efficiency of energy conversion increased with time of heating, reaching 100% after 50 min. The heat power output per unit area was 750 W/m 2 for steel fibre cement, compared with 340 W/m 2 for a metal wire with the same resistance. The use of carbon fibres or graphite particles in place of steel fibres resulted in less effective heating, due to the higher resistivity.
Synthesis of Aminobenzoic Acid-Based Nonpeptide Templates: Applications in Peptidomimetic Drug Discovery (2003)

Aminobenzoic acid-based congeners are emerging as a useful class of nonpeptide templates for applications in peptidomimetic drug discovery. Exemplifying such compounds are recently described antagonists or inhibitors of several receptor and signal transduction protein targets, including, integrin gpIIbIIIa receptor antagonists (1), Ras farnesyl protein transferase inhibitors (2), Src SH2 domain antagonists (3), and growth hormone-releasing peptide (GHRP) receptor agonists (4). Representative examples of these compounds are shown in Fig. 1. Fig. 1. Chemical structures of integrin receptor antagonist, Ras famesyl protein transferase inhibitor, Src SH2 domain antagonist, and GHRP receptor agonist leads, which incorporate aminobenzoic acid-based nonpeptide templates.
Queuing Models for Analysis of Traffic Adaptive Signal Control (2007)

Microsimulation models are normally used to evaluate traffic-adaptive signal control systems. This paper develops an analytical approach for this evaluation based on queuing models. In particular, a queuing model is developed for a simplified adaptive control strategy that is based on rolling horizon scheme, in which a signal serves two movements alternatively. In this strategy, the first movement is served until the queue dissipates, then the second movement is served until the queue dissipates, then the signal goes back to serving the first movement, and this cyclic process repeats. A numerical algorithm is developed in a stochastic context to compute steady-state performance measures such as average delays and expected queue lengths. These results are compared with simulation-based results, and indeed, the analytically derived numerical method predicts well the simulation results
Hybrid row-column partitioning in teradata <sub>®</sub> (2016)

Data partitioning is an indispensable ingredient of database systems due to the performance improvement it can bring to any given mixed workload. Data can be partitioned horizontally or vertically. While some commercial proprietary and open source database systems have one flavor or mixed flavors of these partitioning forms, Teradata Database offers a unique hybrid row-column store solution that seamlessly combines both of these partitioning schemes. The key feature of this hybrid solution is that either row, column, or combined partitions are all stored and handled in the same way internally by the underlying file system storage layer. In this paper, we present the main characteristics and explain the implementation approach of Teradata's row-column store. We also discuss query optimization techniques applicable specifically to partitioned tables. Furthermore, we present a performance study that demonstrates how different partitioning options impact the performance of various queries.
Dissecting eukaryotic translation and its control by ribosome density mapping (2005)

Translation of an mRNA is generally divided into three stages: initiation, elongation and termination. The relative rates of these steps determine both the number and position of ribosomes along the mRNA, but traditional velocity sedimentation assays for the translational status of mRNA determine only the number of bound ribosomes. We developed a procedure, termed Ribosome Density Mapping (RDM), that uses site-specific cleavage of polysomal mRNA followed by separation on a sucrose gradient and northern analysis, to determine the number of ribosomes associated with specified portions of a particular mRNA. This procedure allows us to test models for translation and its control, and to examine properties of individual steps of translation in vivo. We tested specific predictions from the current model for translational control of GCN4 expression in yeast and found that ribosomes were differentially associated with the uORFs elements and coding region under different growth conditions, consistent with this model. We also mapped ribosome density along the ORF of several mRNAs, to probe basic kinetic properties of translational steps in yeast. We found no detectable decline in ribosome density between the 5' and 3' ends of the ORFs, suggesting that the average processivity of elongation is very high. Conversely, there was no queue of ribosomes at the termination site, suggesting that termination is not very slow relative to elongation and initiation. Finally, the RDM results suggest that less frequent initiation of translation on mRNAs with longer ORFs is responsible for the inverse correlation between ORF length and ribosomal density that we observed in a global analysis of translation. These results provide new insights into eukaryotic translation in vivo.
A novel approach to synthesize polymers for potential photodynamic therapy: from benzenedinitrile to phthalocyanine (2014)

RAFT synthesized polymers with benzenedinitrile side chains were converted to phthalocyanine conveniently, providing a novel method for making PDT polymers.
Reassembly of Nucleosomes at the MLH1 Promoter Initiates Resilencing Following Decitabine Exposure (2013)

Hypomethylating agents reactivate tumor suppressor genes that are epigenetically silenced in cancer. Inevitably these genes are resilenced, leading to drug resistance. Using the MLH1 tumor suppressor gene as a model, we showed that decitabine-induced re-expression was dependent upon demethylation and eviction of promoter nucleosomes. Following decitabine withdrawal, MLH1 was rapidly resilenced despite persistent promoter demethylation. Single molecule analysis at multiple time points showed that gene resilencing was initiated by nucleosome reassembly on demethylated DNA and only then was followed by remethylation and stable silencing. Taken together, these data establish the importance of nucleosome positioning in mediating resilencing of drug-induced gene reactivation and suggest a role for therapeutic targeting of nucleosome assembly as a mechanism to overcome drug resistance.
Interspinous Process Segmental Spinal Instrumentation (1984)

A method for interspinous segmental spinal instrumentation (ISSI) is described and the laboratory testing and early clinical results are reported. The method utilizes a button-wire implant that is passed through the thickest and strongest part of the base of the spinous process. Tension tests show the stress-relieving qualities of the implant improve pullout strength 47% over simple wire fixation of the spinous process. Tests on the scoliosis simulator show that the interspinous instrumented spine resists high compressive loads to failure, comparing favorably with other systems tested. Early clinical experience with the implant for a wide variety of uses has been encouraging. The authors use ISSI as their procedure of choice in idiopathic and congenital scoliosis.
Reassessment on the development of children with disability in Malaysia. (2008)

This is a cohort study investigating the profile of children with disability registered with the primary health care clinics in Malaysia. The purpose of the study was to determine whether reassessment on the development of children with disability under rehabilitation should be done at three months interval or six months interval. Secondary data from the pilot project conducted by the Family Health Development Division, Ministry of Health Malaysia was used in this study. The study was carried out for seven months from 1st August 2004 until 28th February 2005. A total of 168 disabled children followed up for six months were selected in this study. Schedule of Growing Scale (SGS) II was the tool used for analysis. Results showed a statistically significant difference in the mean total SGS score at six months interval but not at three months interval. The result suggests that reassessment on children with Down Syndrome, Autism, Cerebral Palsy, mental retardation and delayed speech under rehabilitation should be carried out every six months while children with gross developmental delay and slow learner might need a longer interval for reassessment.
Optische Effekte freier Träger in SnO2-Schichten (1962)

Das Reflexionsvermögen n-leitender SnO 2 -Schichten mit Trägerkonzentrationen zwischen (1·10 20 und 6·10 20 ) /cm 3 wurde bis zu Wellenlängen von 12 μ untersucht und die Ergebnisse unter Einbeziehung von HALL-Messungen mit der DRUDEShcen Theorie verglichen. Für λ &gt; 4 μ entsprechend ω &lt; 1/τ (ω = Frequenz des Lichtes, τ = mittlere Stoßzeit der Elektronen) ergab sich eine gute Übereinstimmung mit der Theorie. Im Gültigkeitsbereich der quantenmechanischen Absorptionstheorien, d. h. für λ &lt; 4 μ, traten gewisse Abweichungen auf, die vermutlich durch Störstellenstreuung bedingt sind.
Guided waves on sinusoidally-modulated reactance surfaces (1959)

A rigorous solution is derived for the propagation characteristics and field distributions of waves guided by a plane surface which possesses a surface reactance modulated sinusoidally in the propagation direction. The explicit field amplitudes and the determinantal equation for the propagation wavenumber are expressed in a continued fraction form which is rapidly convergent for all values of modulation. Numerical results are obtained for both surface wave (modal) and leaky wave (nonmodal) solutions. The relevance of these studies to high-gain modulated surface-wave antennas is discussed.
Untargeted Metabolomics Identifies Enterobiome Metabolites and Putative Uremic Toxins as Substrates of Organic Anion Transporter 1 (Oat1) (2011)

Untargeted metabolomics on the plasma and urine from wild-type and organic anion transporter-1 (Oat1/Slc22a6) knockout mice identified a number of physiologically important metabolites, including several not previously linked to Oat1-mediated transport. Several, such as indoxyl sulfate, derive from Phase II metabolism of enteric gut precursors and accumulate in chronic kidney disease (CKD). Other compounds included vitamins (pantothenic acid, 4-pyridoxic acid), urate, and metabolites in the tryptophan and nucleoside pathways. Three metabolites, indoxyl sulfate, kynurenine, and xanthurenic acid, were elevated in the plasma and interacted strongly and directly with Oat1 in vitro with IC50 of 18, 12, and 50 μM, respectively. A pharmacophore model based on several identified Oat1 substrates was used to screen the NCI database and candidate compounds interacting with Oat1 were validated in an in vitro assay. Together, the data suggest a complex, previously unidentified remote communication between the gut microbiome, Phase II metabolism in the liver, and elimination via Oats of the kidney, as well as indicating the importance of Oat1 in the handling of endogenous toxins associated with renal failure and uremia. The possibility that some of the compounds identified may be part of a larger remote sensing and signaling pathway is also discussed.
Removal of O-Benzyl Blocking Groups with Trifluoroacetic Acid<sup>1</sup> (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleRemoval of O-Benzyl Blocking Groups with Trifluoroacetic Acid1John P. Marsh Jr. and Leon GoodmanCite this: J. Org. Chem. 1965, 30, 7, 2491–2492Publication Date (Print):July 1, 1965Publication History Published online1 May 2002Published inissue 1 July 1965https://pubs.acs.org/doi/10.1021/jo01018a528https://doi.org/10.1021/jo01018a528research-articleACS PublicationsRequest reuse permissionsArticle Views808Altmetric-Citations43LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Extended Baryonic Halo of NGC 3923 (2017)

Galaxy halos and their globular cluster systems build up over time by the accretion of small satellites. We can learn about this process in detail by observing systems with ongoing accretion events and comparing the data with simulations. Elliptical shell galaxies are systems that are thought to be due to ongoing or recent minor mergers. We present preliminary results of an investigation of the baryonic halo—light profile, globular clusters, and shells/streams—of the shell galaxy NGC 3923 from deep Dark Energy Camera (DECam) g and i-band imaging. We present the 2D and radial distributions of the globular cluster candidates out to a projected radius of about 185 kpc, or ∼ 37 R e , making this one of the most extended cluster systems studied. The total number of clusters implies a halo mass of M h ∼ 3 × 10 13 M ⊙ . Previous studies had identified between 22 and 42 shells, making NGC 3923 the system with the largest number of shells. We identify 23 strong shells and 11 that are uncertain. Future work will measure the halo mass and mass profile from the radial distributions of the shell, N-body models, and line-of-sight velocity distribution (LOSVD) measurements of the shells using the Multi Unit Spectroscopic Explorer (MUSE).
Lateral Root Pruning – A Promising Forest Nursery Practice (1968)

The regeneration and growth of pruned roots of Douglas-fir seedlings were studied under nursery conditions. Root pruning did not decrease the height increment of seedlings. For the best development of dense and compact root systems, the bottom pruning should be done early in spring and the side pruning around the middle of June, on both sides of the row simultaneously.
Application of a New Dynamic Flow Model for Investigating the Biocompatibility of Modified Surfaces (2000)

An in vitro model was developed to compare the biocompatibility of four different coating methods (three heparin and one nonheparin) under hemodynamic conditions. Fresh human donor blood (heparin 5 IU/ml) was recirculated in a standardized experimental circuit. All circuit components were either coated or remained uncoated for control purposes. The aim of the study was to investigate a wide spectrum of effects on blood; coagulation parameters (e.g., fibrinogen, ATIII, thrombin-antithrombin-complex), complement parameters (C1rsC1 Inh, C3b(Bb)P, SC5b-9, C5a), differential blood analyses, platelet activation (flow cytometric investigations), PF 4, and PMN-elastase release were examined by showing possible trends. All heparin coated systems reduced platelet stimulation in comparison to untreated biomaterials. Leukocyte activation was reduced to different degrees depending upon the coating method used. Complement activation was markedly reduced by all coated systems. The results obtained indicate that the pump driven, dynamic blood flow model is suitable to characterize the biocompatibility of surface modified biomaterials. Advantages lie in the integration of the different polymers as parts of the circuit, the low priming volume, and the generation of blood flow conditions similar to those that occur in clinical applications.
Phase Transition for Aqueous Solution of Polyelectrolyte Complex Containing N-lsopropylacrylamide. (1997)

カチオン性もしくはアニオン性電解質モノマーとN-イソプロピルアクリルアミド (NIPAAM) の共重合体から作製した高分子電解質錯体 (PEC) は, pH, 塩濃度に加え温度の変化に応答してその構造を変化させることが期待される. 本研究ではこの熱応答性PEC水溶液の熱転移挙動について検討を行い, 以下の結果を得た. 電解質モノマーを導入した共重合体水溶液の転移温度は, 電解質モノマーの導入率, 溶液のpHにより変化した. PECを形成させた場合, 転移温度は単独系に比べ大きく低下した. これは, PECを形成することにより. ポリマーに脱水和を伴う自由度の低下が生ずるため全体として疎水性が増加し, その結果として熱による相転移が起こりやすくなったためと考えられる. また, 少量の塩を添加すると, 静電遮蔽効果によりPECの形成が妨げられ転移温度が上昇したが, さらに塩を添加すると塩析効果により逆に転移温度の低下が観察された.
Transcriptional activation of the Listeria monocytogenes hemolysin gene in Bacillus subtilis (1992)

The prfA gene of Listeria monocytogenes was recently reported to be required for expression of hly, which encodes a pore-forming hemolysin essential for pathogenicity (M. Leimeister-Wachter, C. Haffner, E. Domann, W. Goebel, and T. Chakraborty, Proc. Natl. Acad. Sci. USA 87:8336-8340, 1990). We demonstrate here that a hly-lacZ fusion introduced into Bacillus subtilis is strongly activated when the prfA gene product is supplied in trans under the control of an isopropyl-beta-D-thiogalactopyranoside-inducible promoter, Pspac. Moreover, the PrfA-dependent activation of hly is abolished by point mutations in a 14-bp DNA palindromic sequence present in the 5' upstream region of hly. This indicates that PrfA is both necessary and sufficient for hly transcriptional activation and establishes the palindrome as the likely target sequence for PrfA interaction. The presence of a palindrome in the upstream regions of three additional L. monocytogenes genes clustered near hly suggests that PrfA may serve as a transcriptional activator for a major virulence regulon of L. monocytogenes. In addition, the ability of PrfA to activate its target promoters effectively in B. subtilis suggests that further analysis of this regulon and perhaps other aspects of L. monocytogenes gene regulation might be carried out in part through reconstruction experiments in B. subtilis.