gebhart/patrepeval huggingface dataset:
Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes (2015)

Here, we introduce a new serum-free defined medium (SPM) that supports the cultivation of human pluripotent stem cells (hPSCs) on recombinant human vitronectin-N (rhVNT-N)-coated dishes after seeding with either cell clumps or single cells. With this system, there was no need for an intervening sequential adaptation process after moving hPSCs from feeder layer-dependent conditions. We also introduce a micropatterned dish that was coated with extracellular matrix by photolithographic technology. This procedure allowed the cultivation of hPSCs on 199 individual rhVNT-N-coated small round spots (1 mm in diameter) on each 35-mm polystyrene dish (termed "patterned culture"), permitting the simultaneous formation of 199 uniform high-density small-sized colonies. This culture system supported controlled cell growth and maintenance of undifferentiated hPSCs better than dishes in which the entire surface was coated with rhVNT-N (termed "non-patterned cultures"). Non-patterned cultures produced variable, unrestricted cell proliferation with non-uniform cell growth and uneven densities in which we observed downregulated expression of some self-renewal-related markers. Comparative flow cytometric studies of the expression of pluripotency-related molecules SSEA-3 and TRA-1-60 in hPSCs from non-patterned cultures and patterned cultures supported this concept. Patterned cultures of hPSCs allowed sequential visual inspection of every hPSC colony, giving an address and number in patterned culture dishes. Several spots could be sampled for quality control tests of production batches, thereby permitting the monitoring of hPSCs in a single culture dish. Our new patterned culture system utilizing photolithography provides a robust, reproducible and controllable cell culture system and demonstrates technological advantages for the mass production of hPSCs with process quality control.
Video processing techniques for traffic flow monitoring: A survey (2011)

Video-based traffic flow monitoring is a fast emerging field based on the continuous development of computer vision. A survey of the state-of-the-art video processing techniques in traffic flow monitoring is presented in this paper. Firstly, vehicle detection is the first step of video processing and detection methods are classified into background modeling based methods and non-background modeling based methods. In particular, nighttime detection is more challenging due to bad illumination and sensitivity to light. Then tracking techniques, including 3D model-based, region-based, active contour-based and feature-based tracking, are presented. A variety of algorithms including MeanShift algorithm, Kalman Filter and Particle Filter are applied in tracking process. In addition, shadow detection and vehicles occlusion bring much trouble into vehicle detection, tracking and so on. Based on the aforementioned video processing techniques, discussion on behavior understanding including traffic incident detection is carried out. Finally, key challenges in traffic flow monitoring are discussed.
Characterization of Shiga Toxin-Producing <i>Escherichia coli</i> Strains Isolated from Human Patients in Germany over a 3-Year Period (2004)

We have investigated 677 Shiga toxin-producing Escherichia coli (STEC) strains from humans to determine their serotypes, virulence genes, and clinical signs in patients. Six different Shiga toxin types (1, 1c, 2, 2c, 2d, and 2e) were distributed in the STEC strains. Intimin (eae) genes were present in 62.6% of the strains and subtyped into intimins alpha1, beta1, gamma1, epsilon, theta, and eta. Shiga toxin types 1c and 2d were present only in eae-negative STEC strains, and type 2 was significantly (P < 0.001) more frequent in eae-positive STEC strains. Enterohemorrhagic E. coli hemolysin was associated with 96.2% of the eae-positive strains and with 65.2% of the eae-negative strains. Clinical signs in the patients were abdominal pain (8.7%), nonbloody diarrhea (59.2%), bloody diarrhea (14.3%), and hemolytic-uremic syndrome (HUS) (3.5%), and 14.3% of the patients had no signs of gastrointestinal disease or HUS. Infections with eae-positive STEC were significantly (P < 0.001) more frequent in children under 6 years of age than in other age groups, whereas eae-negative STEC infections dominated in adults. The STEC strains were grouped into 74 O:H types by serotyping and by PCR typing of the flagellar (fliC) genes in 221 nonmotile STEC strains. Eleven serotypes (O157:[H7], O26:[H11], O103:H2, O91:[H14], O111:[H8], O145:[H28], O128:H2, O113:[H4], O146:H21, O118:H16, and O76:[H19]) accounted for 69% of all STEC strains. We identified 41 STEC strains belonging to 31 serotypes which had not previously been described as human STEC. Twenty-six of these were positive for intimins alpha1 (one serotype), beta1 (eight serotypes), epsilon (two serotypes), and eta (three serotypes). Our study indicates that different types of STEC strains predominate in infant and adult patients and that new types of STEC strains are present among human isolates.
Total System Concept for Manufacturing Injection Molded Glass Reinforced Polyester Components (1977)

<div class="htmlview paragraph">A unique method for manufacturing glass-fiber reinforced polyester automotive components has been developed by USM Corporation. This method referred to as the Total System Concept, is unique in that it makes total integration of injection molding and continuous material mixing possible.</div> <div class="htmlview paragraph">The future growth of glass-fiber reinforced plastics will be influenced by equipment and material developments which improve the performance and economics of end use applications. The ‘Total System’ offers a means of reaching this growth potential.</div> <div class="htmlview paragraph">The ‘Total System’ has been fully developed and tested, and is a functional approach to manufacturing injection molded, glass fiber reinforced polyester components at highly economic levels.</div>
Coupled Biaxial Films (1964)

Biaxial films were proposed by Pugh for storage elements of a noncoincident memory. In this paper, it is demonstrated that two identical uniaxial films with their easy axes at a right angle and driven by interposed striplines that carry currents also exhibit biaxial properties.
SEMI-SYSTOLIC ARRAY BASED MOTION ESTIMATION PROCESSOR DESIGN (1995)

This paper presents a new VLSI architecture for full-search block matching algorithm. The proposed architecture has two specific features: (1) it lhas a processor element (PE) array which provides sufficient computational power, where PE&apos;s work in a semi-systolic style and (2) it contains stream memory banks which provide scheduled data flow to reduce idle operations within PE array. By exploiting broadcasting and local data communications, hardware efficiency of the proposed architecture can be up to 1O(P%, which outperforms those systolic-array solutions found in the literature. Many architectural solutions for implementing block maching
The use of reduced healing times on ITI<sup>®</sup> implants with a sandblasted and acid‐etched (SLA) surface: (2002)

ITI dental implants are available with two bone-anchoring surfaces, a titanium plasma-sprayed (TPS) surface, and a recently introduced sandblasted and acid-etched (SLA) surface. Cell culture and animal tests demonstrate that the SLA surface stimulates bone cell differentiation and protein production, has large amounts of bone-to-implant contact, and results in large removal torque values in functional testing of the bone contact. As a result of these studies, a prospective human clinical trial was initiated to determine whether the 4.1 mm diameter SLA ITI solid screw implants could be predictably and safely restored as early as six weeks after implant placement surgery. The protocol restricted the use of the reduced healing time to a) healthy patients with sufficient bone volume to surround the implant, and b) those patients who had good bone quality (classes I-III) at the implant recipient site. Patients with poorer bone quality (class IV) did not have restorations until 12 weeks after implant placement. The clinical trial is an ongoing multicenter trial, with six centers in four countries, and with follow-up over five years. The primary outcome variable was abutment placement with a 35 Ncm force, with no countertorque and no pain or rotation of the implant. A secondary outcome was implant success, as defined by no mobility, no persistent pain or infection, and no peri-implant radiolucency. To date, 110 patients with 326 implants have completed the one-year post-loading recall visit, while 47 patients with 138 implants have completed the two-year recall. Three implants were lost prior to abutment connection. Prosthetic restoration was commenced after shortened healing times on 307 implants. The success rate for these implants, as judged by abutment placement, was 99.3% (with an average healing time of 49 days). Life table analyses demonstrated an implant success rate of 99.1%, both for 329 implants at one year and for 138 implants at two years. In the 24-month period after restoration, no implant losses were reported for the 138 implants. These results demonstrate that, under defined conditions, solid screw ITI implants with an SLA endosseous surface can be restored after approximately six weeks of healing with a high predictability of success, defined by abutment placement at 35 Ncm without countertorque, and with subsequent implant success rates of greater than 99% two years after restoration.
A Distributed Collaborative Product Customization System Based on Web3D (2007)

The paper presents a distributed collaborative product customization system based on Web3D. It is related to the integration of both the three-dimensional and the two-dimensional visible information of the product under the web environment, which provides distributed collaborative product customization for product users in a virtual environment. The article focuses on the system architecture, the creation of distributed collaborative customization environment, the product customization and the virtual display technology.
Three-dimensional photoacoustic tomography of finger joint: from phantom experiment to in vivo study (2009)

In this study, we present phantom experiments and in-vivo human examination to evaluate the possibility of threedimensional (3D) photoacoustic imaging of the finger joints in a spherical scanning geometry. The phantom results show that 1mm thick "cartilage" can be accurately differentiated from the "bones" with a 1 MHz transducer. Results based on in-vivo finger joint study show that major anatomical features in the joint can be imaged, and the cartilages associated with the joint can be differentiated well from the phalanx. The results shown in this paper suggest that 3D PAT in a spherical scanning geometry has the potential to be used for early diagnosis of joint diseases
Serum Lipoprotein Pattern in Rats, Dogs and Monkeys, Including Method Comparison and Influence of Menstrual Cycle in Monkeys (1993)

The serum lipoprotein pattern (HDL-, LDL- and VLDL-cholesterol) was determined in normal untreated rats, dogs and monkeys using the density gradient centrifugation method, and compared with that in humans. The serum of rats (Wistar) and dogs (Beagle) was found to contain mainly HDL-cholesterol, whereas the serum of monkeys (Macaca fascicularis) consists of similar amounts of HDL-cholesterol and LDL-cholesterol, as in humans. In contrast to rats and dogs, monkeys showed a very low amount of serum VLDL-cholesterol. Comparison of four different methods (density gradient centrifugation, FPLC, agarose gel electrophoresis and precipitation methods) for determination of HDL- and LDL-cholesterol in monkey serum showed that density gradient centrifugation and FPLC-methods have the highest accuracy and recovery rate. The FPLC-method offers the extra advantages that it requires less sample volume and is less laborious. In a separate experiment, the influence of the menstrual cycle on the serum lipoprotein pattern was investigated in female monkeys. No changes in total cholesterol, HDL- or LDL-cholesterol were detected in serum samples obtained in the follicular and luteal phases of the cycle. In conclusion, monkeys (Macaca fascicularis) may be more suitable than rats or dogs for the comparison of the effect of different compounds on serum lipoproteins in pharmacological and toxicological studies, because the lipoprotein pattern in this species is similar to that of humans.
Donor Origin of BK Virus in Renal Transplantation and Role of HLA C7 in Susceptibility to Sustained BK Viremia (2005)

In a previous study, we performed serial BK virus (BKV), polymerase chain reaction (PCR) and detected active BKV infection in 70 (35.4%) of 198 renal transplant recipients. In the current study, pre-transplant donor and recipient samples were analyzed for BKV antibody titer and HLA alleles. Donor antibody titer was inversely proportional to onset of viruria, p<0.001, directly proportional to duration of viruria, p=0.014 and directly proportional to peak urine viral titer p=0.005. Recipient pairs receiving kidneys from the same donor were concordant for BKV infection, p=0.017, and had matched sequences of segments of the NCCR and VP1 genes that tended to vary among recipients of kidneys from different donors. We did not see an association of HLA A, B, or DR, HLA allele mismatches or total HLA mismatches and BK infection. However, all 11 recipients with sustained BK viremia received kidneys from donors lacking HLA C7, and 10 recipients also lacked C7. These findings derive from the largest and most comprehensive prospective study of BKV infection in renal transplant recipients performed to date. Our data support donor origin for early BKV infection in kidney transplant recipients, and suggest that a specific HLA C locus may be associated with failure to control BKV infection.
Significance of Hepatitis B Viremia Levels Determined by A Quantitative Polymerase Chain Reaction Assay in Patients With Hepatitis B E Antigen–Negative Chronic Hepatitis B Virus Infection (2003)

Serum HBV DNA levels evaluated by sensitive quantitative PCR assays can be used for differentiation between HBeAg-negative chronic hepatitis B and inactive hepatitis B surface antigen carrier state, but the cut-off level should be set at approximately 30,000 copies/ml and certainly lower than the recently suggested level of 100,000 copies/ml.
The program decision logic approach to predicated execution (1999)

Modern compilers must expose sufficient amounts of Instruction-Level Parallelism (ILP) to achieve the promised performance increases of superscalar and VLIW processors. One of the major impediments to achieving this goal has been inefficient programmatic control flow. Historically, the compiler has translated the programmer's original control structure directly into assembly code with conditional branch instructions. Eliminating inefficiencies in handling branch instructions and exploiting ILP has been the subject of much research. However, traditional branch handling techniques cannot significantly alter the program's inherent control structure. The advent of predication as a program control representation has enabled compilers to manipulate control in a form more closely related to the underlying program logic. This work takes full advantage of the predication paradigm by abstracting the program control flow into a logical form referred to as a program decision logic network. This network is modeled as a Boolean equation and minimized using modified versions of logic synthesis techniques. After minimization, the more efficient version of the program's original control flow is re-expressed in predicated code. Furthermore, this paper proposes extensions to the HPL PlayDoh predication model in support of more effective predicate decision logic network minimization. Finally, this paper shows the ability of the mechanisms presented to overcome limits on ILP previously imposed by rigid program control structure.
<i>In vivo</i> and Microarray Analysis of Rexinoid-Responsive Anaplastic Thyroid Carcinoma (2008)

LGD1069 therapy decreases proliferation in an anaplastic thyroid cancer cell line that expresses retinoid X receptor-gamma, and this effect is confirmed with decreased tumor size in vivo in a nude mouse model. ANGPTL4 is increased in DRO in response to LGD1069 and may be a potential mediator of the effects of rexinoid treatment.
An analysis of the role of large conventions in the tourism industry (1983)

It has become accepted, almost as an article of faith in the tourism industry, that a major option, open to both large cities and traditional resorts which wish to maintain or increase their tourism earnings, is the generation of large scale conference tourism through the construction of large convention or congress centres. Implicit in this assumption is a belief that large scale conventions will continue to occupy an important place in the tourism industry.
Pump-down completion of satellite underwater wells (1968)

Humble Oil and Refining Co. recently demonstrated an important advancement in subsea completion technology by remotely completing a satellite underwater well in its brand Isle Block 16 Field with pump-down tools. This was industry's first known attempt to perform completion operations with the rig off location. Pump-down tools and techniques were used to perforate and consolidate the producing sand at 10,100-ft TVD. This pump-down completion along with onshore tests simulating other pump-down completion-workover operations demonstrates the capability to remotely perform virtually all through-tubing type well servicing operations necessary to complete and maintain satellite subsea wells. This technology, in addition to significantly lowering completion costs of subsea wells, will substantially reduce maintenance costs by eliminating the need for many vertical re-entry workovers. In addition, ultimate recovery from a well may be increased due to the ability to inexpensively perform workovers which would not be economically attractive using conventional workover methods utilizing a mobile rig.
Near-Zero-Power Temperature Sensing via Tunneling Currents Through Complementary Metal-Oxide-Semiconductor Transistors (2017)

Temperature sensors are routinely found in devices used to monitor the environment, the human body, industrial equipment, and beyond. In many such applications, the energy available from batteries or the power available from energy harvesters is extremely limited due to limited available volume, and thus the power consumption of sensing should be minimized in order to maximize operational lifetime. Here we present a new method to transduce and digitize temperature at very low power levels. Specifically, two pA current references are generated via small tunneling-current metal-oxide-semiconductor field effect transistors (MOSFETs) that are independent and proportional to temperature, respectively, which are then used to charge digitally-controllable banks of metal-insulator-metal (MIM) capacitors that, via a discrete-time feedback loop that equalizes charging time, digitize temperature directly. The proposed temperature sensor was integrated into a silicon microchip and occupied 0.15 mm<sup>2</sup> of area. Four tested microchips were measured to consume only 113 pW with a resolution of 0.21 °C and an inaccuracy of ±1.65 °C, which represents a 628× reduction in power compared to prior-art without a significant reduction in performance.
Gaucher's Disease and Cancer: A Sphingolipid Perspective (2013)

Gaucher's disease is a sphingolipidosis characterized by a specific deficiency in an acidic glucocerebrosidase, which results in aberrant accumulation of glucosylceramide primarily within the lysosome. Gaucher's disease has been correlated with cases of myeloma, leukemia, glioblastoma, lung cancer, and hepatocellular carcinoma, although the reasons for the correlation are currently being debated. Some suggest that the effects of Gaucher's disease may be linked to cancer, while others implicate the therapies used to treat Gaucher's disease. This debate is not entirely surprising, as the speculations linking Gaucher's disease with cancer fail to address the roles of ceramide and glucosylceramide in cancer biology. In this review, we will discuss, in the context of cancer biology, ceramide metabolism to glucosylceramide, the roles of glucosylceramide in multidrug-resistance, and the role of ceramide as an anticancer lipid. This review should reveal that it is most practical to associate elevated glucosylceramide, which accompanies Gaucher's disease, with the progression of cancer. Furthermore, this review proposes that the therapies used to treat Gaucher's disease, which augment ceramide accumulation, are likely not linked to correlations with cancer.
Methods in alkaloid synthesis. Imino ethers as donors in the Michael reaction (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMethods in alkaloid synthesis. Imino ethers as donors in the Michael reactionBarry M. Trost and Robert A. KunzCite this: J. Am. Chem. Soc. 1975, 97, 24, 7152–7157Publication Date (Print):November 1, 1975Publication History Published online1 May 2002Published inissue 1 November 1975https://doi.org/10.1021/ja00857a030RIGHTS & PERMISSIONSArticle Views968Altmetric-Citations54LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (780 KB) Get e-Alerts Get e-Alerts
Species differences in insulin secretory responses during hemorrhagic shock. (1976)

Insulin secretory rates (ISR) during intravenous glucose tolerance tests (IVGTT's) were measured in six dogs subjected to hemorrhagic shock and were compared to ISR's from five monkeys subjected to shock of comparable severity. ISR's also were measured in normotensive control dogs and monkeys subjected to the same blood sampling protocol. A sixfold increase in ISR occurred in shocked dogs after glucose loading; however, no ISR response occurred in monkeys subjected to hemorrhage. It is concluded that marked species differences exist in the insulin-glucose metabolic responses to shock. In addition, the dog would appear to be an inappropriate experimental animal as applied to trauma-insulin metabolism in man.