gebhart/patrepeval huggingface dataset:
Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia (2011)

The combination of CBMC infusion and rehabilitation training may be a safe and effective treatment for ataxia, which dramatically improves patients' functional symptoms. These data support expanded double blind, placebo-controlled studies for these treatment modalities.
Photoprotection of Patients Sensitive to Short and/or Long Ultraviolet Light with Dihydroxyacetone/Naphthoquinone (1974)

The topical sunscreen, dihydroxyacetone/lawsone (DHA/lawsone), was tested on 18 patients with photosensitivity disorders. Separate sequential application of the components increased sunlight tolerance in these patients twelvefold, thus providing four times greater protection against sunlight than did the previous technique employing mixtures of the components. This sunscreen, which is chemically bound to the stratum corneum, provides both long and short ultraviolet photoprotection.
Ion-selective electrode for the determination of phencyclidine (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIon-selective electrode for the determination of phencyclidineCharles R. Martin and Henry. FreiserCite this: Anal. Chem. 1980, 52, 11, 1772–1774Publication Date (Print):September 1, 1980Publication History Published online1 May 2002Published inissue 1 September 1980https://doi.org/10.1021/ac50061a058RIGHTS & PERMISSIONSArticle Views112Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (424 KB) Get e-Alertsclose Get e-Alerts
Heteroatom-assisted substitution of acyclic secondary tosylates with lithium dialkylcuprates. An expedient route to stereochemically defined deoxypropionate and related biosynthetic subunits (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHeteroatom-assisted substitution of acyclic secondary tosylates with lithium dialkylcuprates. An expedient route to stereochemically defined deoxypropionate and related biosynthetic subunitsS. Hanessian, B. Thavonekham, and B. DeHoffCite this: J. Org. Chem. 1989, 54, 25, 5831–5833Publication Date (Print):December 1, 1989Publication History Published online1 May 2002Published inissue 1 December 1989https://pubs.acs.org/doi/10.1021/jo00286a001https://doi.org/10.1021/jo00286a001research-articleACS PublicationsRequest reuse permissionsArticle Views614Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Prediction of Asphaltene Instability under Gas Injection with the PC-SAFT Equation of State (2005)

Proper prediction of the potential for asphaltene precipitation in deepwater reservoirs represents a challenge for the flow assurance area due to both high intervention costs in case of asphaltene problems and extreme conditions of pressure, temperature, and composition. The probability of asphaltene precipitation increases when light-oil gases are considered for miscible gas injection processes. In this work, the applicability of simple and recombined live oil models using the PC-SAFT equation of state to predict the onset of asphaltene precipitation is demonstrated by studying pressure depletion and gas injection (carbon dioxide (CO2), nitrogen (N2), methane, and ethane) in oil reservoirs. The recombined model input includes the SARA characterization, the oil compositional analysis, and the gas−oil ratio. The pure component PC-SAFT parameters are fitted to the saturated liquid density and vapor pressure data, and the oil/gas pseudocomponent parameters are estimated from molecular-weight-based correlations. The EOS asphaltene parameters are fitted to precipitation data from oil titration with n-alkanes at ambient conditions. When asphaltene experimental data are not available, the asphaltene parameters are tuned to data at reservoir conditions, and then the model is run in a predictive mode. The PC-SAFT model was found to be in qualitative agreement with experimental data for the precipitant/crude oil systems in this study. In the prediction of the asphaltene onset using the model live oil, the results indicate that the ethane and CO2 mass fraction concentrations required to produce asphaltene precipitation is greater than for methane at the same pressure. Simulation results using the recombined model show that N2 addition produces a high asphaltene precipitation effect even at low concentrations. The simulated phase envelope for liquid−liquid asphaltene phase separation shows asphaltene stability bounded by an upper critical solution temperature and a lower critical solution temperature. The approach demonstrates that only molecular size and van der Waals interactions can explain laboratory and field observations of asphaltene phase behavior.
CXCL13 drives spinal astrocyte activation and neuropathic pain via CXCR5 (2016)

Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, we found that CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice. shRNA-mediated inhibition of CXCL13 in the spinal cord persistently attenuated SNL-induced neuropathic pain. Interestingly, CXCL13 expression was suppressed by miR-186-5p, a microRNA that colocalized with CXCL13 and was downregulated after SNL. Spinal overexpression of miR-186-5p decreased CXCL13 expression, alleviating neuropathic pain. Furthermore, SNL induced CXCR5 expression in spinal astrocytes, and neuropathic pain was abrogated in Cxcr5-/- mice. CXCR5 expression induced by SNL was required for the SNL-induced activation of spinal astrocytes and microglia. Intrathecal injection of CXCL13 was sufficient to induce pain hypersensitivity and astrocyte activation via CXCR5 and ERK. Finally, intrathecal injection of CXCL13-activated astrocytes induced mechanical allodynia in naive mice. Collectively, our findings reveal a neuronal/astrocytic interaction in the spinal cord by which neuronally produced CXCL13 activates astrocytes via CXCR5 to facilitate neuropathic pain. Thus, miR-186-5p and CXCL13/CXCR5-mediated astrocyte signaling may be suitable therapeutic targets for neuropathic pain.
Active contour models - a multiscale implementation for anatomical feature delineation in cervical images (2005)

In the work presented here, digital colposcopic images from cervix uteri are subjected to a segmentation algorithm using active contour models at multiresolution levels, on images formed by a Gaussian pyramid (GP). The segmentation aims to outline a specific feature from the cervical images $the transformation zone (TZ), where a possible neoplasia can occur. The process includes an implementation of a boundary-searching snake, based on both image gradient features and region features. The adaptive "snake" initially is executed on the image with the lowest resolution from the GP, aiming to avoid a specific artefact in these images, known as specular reflection (SR). Further, the snake coordinates are propagated to the image with highest resolution from the GP. Under a specific set of parameters, we achieved accurate delineation of the TZ, measured by comparison with pre-defined "ground truth".
Donepezil Significantly Improves Abilities in Daily Lives of Female down Syndrome Patients with Severe Cognitive Impairment: A 24-Week Randomized, Double-Blind, Placebo-Controlled Trial (2011)

Donepezil may effectively and safely improve overall functioning of DS patients with severe cognitive impairment.
Application of Multimedia Technology in Traditional Chinese Medicine Teaching (2013)

The merits and demerits were discussed of applying multimedia technology in the teaching of Traditional Chinese Medicine(TCM) at western medical hospital,and measures were proposed.The characteristics of directness,interactivity,much information,intelligibility and acceptance make multimedia technology to be a better teaching mode in the teaching of TCM.However,due to the abstractness of TCM,the large amount of knowledge points,and the shortness of teaching time,students at western medical colleges and universities still find that TCM is difficult to be understood.Therefore,it needs to further improve the multimedia teaching method and develop creative thinking in students,so as to scientize the teaching methods and contents.
Confronting The Growing Burden Of Chronic Disease: Can The U.S. Health Care Workforce Do The Job? (2009)

The U.S. chronic illness burden is increasing and is felt more strongly in minority and low-income populations: in 2005, 133 million Americans had at least one chronic condition. Prevention and management of chronic disease are best performed by multidisciplinary teams in primary care and public health. However, the future health care work-force is not projected to include an appropriate mix of personnel capable of staffing such teams. To prepare for the growing chronic disease burden, a larger interdisciplinary primary care workforce is needed, and payment for primary care should reward practices that incorporate multidisciplinary teams.
Prospective Vitreous Materials For Infrared Fiber Optics (1982)

Fiber optics operating in the mid-infrared (IR) offer the potential for lower losses and better tolerance to nuclear radiation than current silicate-based fibers. Moreover, mid-IR fibers may be useful for a variety of shorter distance applications such as laser surgery, spot welding, and infrared integrated optics. Although a wide array of potentially highly transmissive mid-IR materials are available in bulk form, most are not suitable for fiber fabrication. Recently, however, a variety of new multicomponent glasses based on the fluorides of heavy metals have been developed, which may offer the best prospects to date for high performance mid-IR fibers. A critical comparison of the advantages as well as the problems associated with various prospective materials and fiber fabrication techniques is given.
Improved auto-extrinsic calibration between stereo vision camera and laser range finder (2020)

This study identifies a way to accurately estimate extrinsic calibration parameters between stereo vision camera and 2D laser range finder (LRF) based on 3D reconstruction of monochromatic calibration board and geometric co-planarity constraints between the views from these two sensors. It supports automatic extraction of plane-line correspondences between camera and LRF using monochromatic board, which is further improved by selecting optimal threshold values for laser scan dissection to extract line features from LRF data. Calibration parameters are then obtained by solving co-planarity constraints between the estimated plane and line. Furthermore, the obtained parameters are refined by minimising reprojection error and error from the co-planarity constraints. Moreover, calibration accuracy is achieved because of extraction of reliable plane-line correspondence using monochromatic board which reduces the impact of range-reflectivity-bias observed in LRF data on checkerboard . As the proposed method supports to automatically extract feature correspondences, it provides a major reduction in time required from an operator in comparison to manual methods. The performance is validated by extensive experimentation and simulation, and estimated parameters from the proposed method demonstrate better accuracy than conventional methods.
ROS1 5-methylcytosine DNA glycosylase is a slow-turnover catalyst that initiates DNA demethylation in a distributive fashion (2009)

Arabidopsis ROS1 belongs to a family of plant 5-methycytosine DNA glycosylases that initiate DNA demethylation through base excision. ROS1 displays the remarkable capacity to excise 5-meC, and to a lesser extent T, while retaining the ability to discriminate effectively against C and U. We found that replacement of the C5-methyl group by halogen substituents greatly decreased excision of the target base. Furthermore, 5-meC was excised more efficiently from mismatches, whereas excision of T only occurred when mispaired with G. These results suggest that ROS1 specificity arises by a combination of selective recognition at the active site and thermodynamic stability of the target base. We also found that ROS1 is a low-turnover catalyst because it binds tightly to the abasic site left after 5-meC removal. This binding leads to a highly distributive behaviour of the enzyme on DNA substrates containing multiple 5-meC residues, and may help to avoid generation of double-strand breaks during processing of bimethylated CG dinucleotides. We conclude that the biochemical properties of ROS1 are consistent with its proposed role in protecting the plant genome from excess methylation.
Evaluation of beta-globin gene therapy constructs in single copy transgenic mice (1997)

Effective gene therapy constructs based on retrovirus or adeno-associated virus vectors will require regulatory elements that direct expression of genes transduced at single copy. Most beta-globin constructs designed for therapy of beta-thalassemias are regulated by the 5'HS2 component of the locus control region (LCR). Here we show that a human beta-globin gene flanked by two small 5'HS2 core elements or flanked by a 5'HS3 (footprints 1-3) core and a 5'HS2 core are not reproducibly expressed in single copy transgenic mice. In addition, low copy transgene concatamers that contain only dimer 5'HS2 cores fail to express, whereas those that contain monomer 5'HS2 cores express at 14% per copy. These data suggest that spacing between HS cores is crucial for LCR activity. We therefore constructed a novel 3.0 kb LCR cassette in which the 5'HS2, 5'HS3 and 5'HS4 cores are each separated by approximately 700 bp. When linked to the 815 bp beta-globin promoter this LCR directs 45% levels of expression from four independent single copy transgenes. However, the 3.0 kb LCR linked to the 265 bp promoter expresses variable levels, averaging 18%, from three single copy transgenes. Our findings suggest that sequences in the distal promoter play a role in single copy transgene activation and that larger LCR and promoter elements are most suitable for gene therapy applications.
Circadian Regulation of Hippocampal Long-Term Potentiation (2005)

The goal of this study is to investigate the possible circadian regulation of hippocampal excitability and long-term potentiation (LTP) measured by stimulating the Schaffer collaterals (SC) and recording the field excitatory postsynaptic potential (fEPSP) from the CA1 dendritic layer or the population spike (PS) from the soma in brain slices of C3H and C57 mice. These 2 strains of mice were of interest because the C3H mice secrete melatonin rhythmically while the C57 mice do not. The authors found that the magnitude of the enhancement of the PS was significantly greater in LTP recorded from night slices compared to day slices of both C3H and C57 mice. They also found significant diurnal variation in the decay of LTP measured with fEPSPs, with the decay slower during the night in both strains of mice. There was evidence for a diurnal rhythm in the input/output function of pyramidal neurons measured at the soma in C57 but not C3H mice. Furthermore, LTP in the PS, measured in slices prepared during the day but recorded during the night, had a profile remarkably similar to the night group. Finally, PS recordings were carried out in slices from C3H mice maintained in constant darkness prior to experimentation. Again, the authors found that the magnitude of the enhancement of the PS was significantly greater in LTP recorded from subjective night slices compared to subjective day slices. These results provide the 1st evidence that an endogenous circadian oscillator modulates synaptic plasticity in the hippocampus.
Incremental generation of projections of CT-volumes (2002)

In order to achieve the illusion of rotation of an object in a 3-D image (density volume), a sequence of projections of the image must be computed. A method that alleviates the need to perform a full-fledged ray-casting operation for each projection in the sequence when the projections are depth coded (range images) is described. The method proceeds incrementally exploiting the coherence between successive projections. The idea behind the incremental method (INC) is that speed can be gained if costly ray-casting methods are as far as possible replaced by 2-D geometric transformations of the currently visible surface points. As is shown, INC significantly reduces the number of ray-casting operations needed. Experiments indicate that a speed up factor of about 10 is achievable. Increasing the angle increment phi diminishes the coherence between successive projections, and thus more rays are required. As phi is further increased, INC gradually degenerates into full ray casting.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Mechanisms of α-Defensin Bactericidal Action: Comparative Membrane Disruption by Cryptdin-4 and Its Disulfide-Null Analogue (2008)

Mammalian alpha-defensins all have a conserved triple-stranded beta-sheet structure that is constrained by an invariant tridisulfide array, and the peptides exert bactericidal effects by permeabilizing the target cell envelope. Curiously, the disordered, disulfide-null variant of mouse alpha-defensin cryptdin-4 (Crp4), termed (6C/A)-Crp4, has bactericidal activity equal to or greater than that of the native peptide, providing a rationale for comparing the mechanisms by which the peptides interact with and disrupt phospholipid vesicles of defined composition. For both live Escherichia coli ML35 cells and model membranes, disordered (6C/A)-Crp4 induced leakage in a manner similar to that of Crp4 but had less overall membrane permeabilizing activity. Crp4 induction of the leakage of the fluorophore from electronegative liposomes was strongly dependent on vesicle lipid charge and composition, and the incorporation of cardiolipin into liposomes of low electronegative charge to mimic bacterial membrane composition conferred sensitivity to Crp4- and (6C/A)-Crp4-mediated vesicle lysis. Membrane perturbation studies using biomimetic lipid/polydiacetylene vesicles showed that Crp4 inserts more pronouncedly into membranes containing a high fraction of electronegative lipids or cardiolipin than (6C/A)-Crp4 does, correlating directly with measurements of induced leakage. Fluorescence resonance energy transfer experiments provided evidence that Crp4 translocates across highly charged or cardiolipin-containing membranes, in a process coupled with membrane permeabilization, but (6C/A)-Crp4 did not translocate across lipid bilayers and consistently displayed membrane surface association. Thus, despite the greater in vitro bactericidal activity of (6C/A)-Crp4, native, beta-sheet-containing Crp4 induces membrane permeabilization more effectively than disulfide-null Crp4 by translocating and forming transient membrane defects. (6C/A)-Crp4, on the other hand, appears to induce greater membrane disintegration.
Caffeine promotes glutamate and histamine release in the posterior hypothalamus (2014)

Histamine neurons are active during waking and largely inactive during sleep, with minimal activity during rapid-eye movement (REM) sleep. Caffeine, the most widely used stimulant, causes a significant increase of sleep onset latency in rats and humans. We hypothesized that caffeine increases glutamate release in the posterior hypothalamus (PH) and produces increased activity of wake-active histamine neurons. Using in vivo microdialysis, we collected samples from the PH after caffeine administration in freely behaving rats. HPLC analysis and biosensor measurements showed a significant increase in glutamate levels beginning 30 min after caffeine administration. Glutamate levels remained elevated for at least 140 min. GABA levels did not significantly change over the same time period. Histamine level significantly increased beginning 30 min after caffeine administration and remained elevated for at least 140 min. Immunostaining showed a significantly elevated number of c-Fos-labeled histamine neurons in caffeine-treated rats compared with saline-treated animals. We conclude that increased glutamate levels in the PH activate histamine neurons and contribute to caffeine-induced waking and alertness.
Measurements of the isotopic composition of UF6 according to the fine structure of the IR absorption spectrum in the ν1 + ν3 band (2017)

Absorption spectra of the Q-branch of the ν1 + ν3 vibrational–rotational band of uranium hexafluoride (UF6) recorded in a range of 1290.0–1292.5 cm–1 using a laser spectrometer based on a quantum cascade laser have been studied. The spectra of samples with a natural isotopic composition (0.7% U235), an enriched sample (90% U235), and their gas mixtures (2, 5, and 20% U235) in a pressure range of 10–70 Torr at a temperature of T = 296 K have been analyzed. The experiments have revealed a highly reproducible fine structure of the recorded spectra. Periodic singularities in the fine-structure spectra have been interpreted as a manifestation of hot band transitions near the Q-branch. Anharmonicity constants X 21, X 31, and X 32 and their combinations X i1 + X i3 (i = 4, 5, 6) have been determined. The characteristic features in the fine-structure spectra and the initial spectrum have been used to determine the isotopic composition of enriched UF6 samples.
3.7 A 40-to-64Gb/s NRZ transmitter with supply-regulated front-end in 16nm FinFET (2016)

Due to increasing bandwidth demand in data centers and telecommunication infrastructures, the maximum data-rate of wireline transceivers is projected to double from 32Gb/s to 64Gb/s while keeping the same power envelope. This paper presents the design of a 64Gb/s NRZ transmitter for short-reach electrical links in a 16nm FinFET process. It is applicable to standards such as CEI-56-VSR/MR, using power efficient techniques that take into considerations FinFET device properties: low DIBL, negligible body effect, low junction capacitance, low channel leakage, high intrinsic gain, high gate capacitance and resistance, high flicker noise, and steep CV curve in accumulation region.