gebhart/patrepeval huggingface dataset:
Flow cytometry of human gynecologic specimens using log chromomycin A3 fluorescence and log 90 degrees light scatter. (1979)

Flow cytometry and electronic cell sorting are being investigated to screen gynecologic specimens for cervical neoplasia. Cellular DNA content is quantitated by Chromomycin A3 fluorescence and cell size is quantitated by 90 degrees light scatter; the logarithms of the measured intensities are used to produce a two parameter histogram. To determine the cell types responsible for signals in various histogram regions, systematic electronic cell sorting is performed. The sorted fractions are sedimented into microscope slides and stained by the Papanicolaou technique. The cells in each fraction are identified by conventional cytomorphologic criteria. Morphologic analysis of sorted cells reveals histogram regions corresponding to specific cell types. One very important region contains the highest concentration of signals from abnormal cells and is therefore the best region to analyze for specimen abnormality. However, because a significant number of signals in this region are from normal cells, specimens cannot be diagnosed by their analysis. Another important histogram region is composed primarily of signals from endocervical columnar and metaplastic cells. The presence of such cells is a good criterion for specimen adequacy, therefore analysis of signals in this region is essential to assess specimen adequacy for automatic screening.
Mapping Temperature-sensitive Mutants of Vesicular Stomatitis Virus by RNA Heteroduplex Formation (1981)

Duplex RNA molecules made by hybridization of virion and mRNA of vesicular stomatitis virus (VSV) were digested with ribonuclease and separated into five size classes, each containing the gene and the mRNA for one of the VSV proteins. Denaturation of the duplexes yielded full size mRNA lacking poly(A) tails. Utilizing duplex formation between the RNAs from VSV temperature-sensitive (ts) mutants and their revertants and subsequent RNase digestion under varying salt conditions, specific cleavages within a certain duplex were seen for representative mutants from complementation groups, III, IV and V. Specific cleavages were not seen for a group II mutant. From these results gene assignments cannot be made for group II; equivocal assignments are made for group III and clear assignments made for group IV and V. The assignment for the group V mutants, however, does not conform to expectations. Nevertheless, from these studies and other published ones, there is the suggestion that interactions may exist between the gene products of complementation groups II and V during VSV transcription and morphogenesis. These results also support the lack of transcriptional splicing for VSV mRNAs.
Effect of tissue inhibitor of the matrix metalloproteinases-2 expression on the growth and spontaneous metastasis of a human melanoma cell line. (1994)

In this study, we examined the effect of expression of tissue inhibitor of metalloproteinases-2 (TIMP-2) on the growth and dissemination of a highly metastatic human melanoma cell line (M24net). M24net melanoma cells express a number of matrix metalloproteinases (MMPs), including gelatinase A and B (MMP-2 and MMP-9) and interstitial collagenase (MMP-1) (A. M. P. Montgomery et al., Cancer Res., 53: 693-700, 1993). The activity of these proteases was effectively down-regulated by transfecting M24net cells with complementary DNA-encoding human TIMP-2. Overexpression of TIMP-2 markedly reduced melanoma growth in the skin of immunodeficient mice but did not prevent these highly malignant cells from spontaneously metastasizing to the lungs and lymph nodes of inoculated mice. We provide a mechanism to account for the growth inhibitory property of TIMP-2 based on its ability to regulate M24net cell growth in three-dimensional interstitial collagen. In the presence of this matrix, M24net cells assume a differentiated morphology and have a reduced growth rate. We present evidence that overexpression of TIMP-2 increases the susceptibility of M24net cells to growth inhibition and morphological differentiation by occluding interstitial collagen.
GESTÃO DE SISTEMA DE INFORMAÇÃO ACADÊMICA: UM ESTUDO DESCRITIVO DA SATISFAÇÃO DOS USUÁRIOS (2005)

RESUMO O uso das tecnologias digitais de comunicação e informação para o gerenciamento das atividades-meio das instituições de ensino superior está se tornando cada vez mais freqüente, possibilitando a realização de inúmeras pesquisas. Entre as investigações sobre esta temática estão os estudos sobre satisfação de usuários de sistemas de informação. A presente pesquisa teve como objetivo investigar o grau de satisfação dos usuários do Sistema de Informação Acadêmico Collegium das Faculdades UNICEN – Campus de Primavera do Leste, MT. Para tanto, sistematizou-se um referencial teórico acerca dos avanços tecnológicos da sociedade da informação, particularizando alguns conceitos de sistemas de informação, reflexões teóricas sobre a satisfação dos usuários e os métodos de avaliação dos referidos sistemas. Este estudo enquadra-se em uma categoria de pesquisa qualitativa denominada estudo descritivo. Na coleta dos dados empre-gou-se a triangulação de diversas técnicas de pesquisa, incluindo entrevistas, análise documental e aplicação de questionário. A análise das evidências empíricas revelou um alto grau de satisfação dos usuários em relação ao sistema utilizado. Apesar disso, a implantação do sistema encontrou alguns entraves que vão desde a falta de planejamento, passando pela falta de treinamento dos usuários, até a ausência de um plano de comunicação que expressasse as vantagens do sistema Collegium. A pesquisa apresenta um conjunto de reflexões que poderá contribuir ao aperfeiçoamento da gestão acadêmica da instituição, bem como ao próprio processo de gestão do sistema analisado.
Effect of Depolymerized Alginates on the Growth of Bifidobacteria (1992)

Journal Article Effect of Depolymerized Alginates on the Growth of Bifidobacteria Get access Hisayoshi Akiyama, Hisayoshi Akiyama Otsuka Chemical Co., Ltd., Division of Food and Beverage Research, Kawauchi, Tokushima 771–01, Japan Search for other works by this author on: Oxford Academic Google Scholar Taeko Endo, Taeko Endo Otsuka Chemical Co., Ltd., Division of Food and Beverage Research, Kawauchi, Tokushima 771–01, Japan Search for other works by this author on: Oxford Academic Google Scholar Ryo Nakakita, Ryo Nakakita Otsuka Chemical Co., Ltd., Division of Food and Beverage Research, Kawauchi, Tokushima 771–01, Japan Search for other works by this author on: Oxford Academic Google Scholar Kousaku Murata, Kousaku Murata Otsuka Chemical Co., Ltd., Division of Food and Beverage Research, Kawauchi, Tokushima 771–01, JapanResearch Institutefor Food Science, Kyoto University, Uji, Kyoto 611, Japan Corresponding author. Search for other works by this author on: Oxford Academic Google Scholar Yoshimasa Yonemoto, Yoshimasa Yonemoto Otsuka Chemical Co., Ltd., Division of Food and Beverage Research, Kawauchi, Tokushima 771–01, Japan Search for other works by this author on: Oxford Academic Google Scholar Kenichi Okayama Kenichi Okayama Otsuka Chemical Co., Ltd., Division of Food and Beverage Research, Kawauchi, Tokushima 771–01, Japan Search for other works by this author on: Oxford Academic Google Scholar Bioscience, Biotechnology, and Biochemistry, Volume 56, Issue 2, 1 January 1992, Pages 355–356, https://doi.org/10.1271/bbb.56.355 Published: 01 January 1992 Article history Received: 13 August 1991 Published: 01 January 1992
The NMR spectra and conformations of cyclic compoundsV: Proton couplings and chemical shifts in bridged cyclobutanes (1972)

Abstract The PMR spectra of twelve pinene derivatives are reported, analysed and assigned. The proton couplings in the bridged cyclobutane group are compared with those of other strained cyclobutanes, and the relationship between 2 J HH and the C.CH 2 .C angle is shown to be anomalous in these systems, suggesting unusually small H. C. H. angles in cyclobutanes. The very large values of 4 J HH (eq‐eq) in buckled cyclobutanes are interpreted in terms of current M. O. theory and also given a simple geometric rationalisation based on the direct mechanism. The various couplings in the pinene skeleton are discussed in terms of present theories and minor conformational effects in these molecules. Substituent chemical shift (SCS) values for Me and OH groups around the pinene skeleton are obtained, and shown not to agree with calculations based on present theories of chemical shifts.
From quantum cloning to quantum key distribution with continuous variables: a review (Invited) (2007)

Quantum information processing with continuous variables is a paradigm that has attracted a growing interest over the past years, partly as a consequence of the prospects for high-rate quantum communication systems based on standard optical telecommunication components. In this overview article, we introduce the concept of quantum continuous variables in optics and then turn to the fundamental impossibility of cloning continuous-variable light states, a result that lies at the heart of quantum key distribution. Then we present state-of-the-art quantum key distribution systems relying on continuous variables, focusing mainly on the protocols using Gaussian-modulated coherent light states and emphasizing the current experimental demonstration of these systems. Finally, we briefly review recent security proofs of these cryptographic protocols.
Antidiabetic therapy and increased risk of hepatocellular carcinoma in chronic liver disease (2009)

Our study confirms that type 2 diabetes mellitus is an independent risk factor for HCC and pre-exists in the majority of HCC patients. Moreover, in male patients with type 2 diabetes mellitus, our data shows a direct association of HCC with insulin and sulphanylureas treatment and an inverse relationship with metformin therapy.
Broadband characterization of indoor powerline channel and its capacity consideration (2005)

For data communication over any medium, it is necessary to determine the characteristics of the communication channel. Powerline channel has been considered as a medium not only for low-rate, control communications, but also for high-speed data communications. In order to implement advanced communication technologies, the complete knowledge of the broadband powerline channel, including the analysis of the channel capacity, are required. This paper strives to widen the understanding of broadband indoor powerline channel by focusing on the properties of indoor powerline channels and its noise behavior. Capacity of the PLC-channel is optimized by using a water-pouring algorithm.
Three-Frame Difference Algorithm Research Based on Mathematical Morphology (2012)

Because of the features of moving target, such as strong speed degeneration and uncertain route, it becomes hard to detect and track the moving target. Although there are many detecting methods for moving target, the noise of detection affects the detecting precision dramatically. In this paper, we extract target by three frame subtraction, and then process the extracting image by the method of mathematical morphology. By this method, the moving target can be detected effectively and the noise is eliminated dramatically. The experimental results show that this method has less calculation amount, higher real-time performance and higher accuracy for moving target detection, and this method can be well applied outdoor.
IKK/NF-κB regulates skeletal myogenesis via a signaling switch to inhibit differentiation and promote mitochondrial biogenesis (2008)

Nuclear factor κB (NF-κB) is involved in multiple skeletal muscle disorders, but how it functions in differentiation remains elusive given that both anti- and promyogenic activities have been described. In this study, we resolve this by showing that myogenesis is controlled by opposing NF-κB signaling pathways. We find that myogenesis is enhanced in MyoD-expressing fibroblasts deficient in classical pathway components RelA/p65, inhibitor of κB kinase β (IKKβ), or IKKγ. Similar increases occur in myoblasts lacking RelA/p65 or IKKβ, and muscles from RelA/p65 or IKKβ mutant mice also contain higher fiber numbers. Moreover, we show that during differentiation, classical NF-κB signaling decreases, whereas the induction of alternative members IKKα, RelB, and p52 occurs late in myogenesis. Myotube formation does not require alternative signaling, but it is important for myotube maintenance in response to metabolic stress. Furthermore, overexpression or knockdown of IKKα regulates mitochondrial content and function, suggesting that alternative signaling stimulates mitochondrial biogenesis. Together, these data reveal a unique IKK/NF-κB signaling switch that functions to both inhibit differentiation and promote myotube homeostasis.
Electrogenic uptake of glutamate and aspartate into glial cells isolated from the salamander (Ambystoma) retina. (1991)

The effects of excitatory amino acids on the membrane current of isolated retinal glial cells (Müller cells) were investigated using whole-cell patch clamping. 2. L-Glutamate evoked an inward current at membrane potentials between -140 and +50 mV. The current was larger at more negative potentials. 3. The glutamate-evoked current was activated by external cations with relative efficacies: Na+ much greater than Li+ greater than K+ greater than Cs+, choline. It was activated by internal cations with relative efficacies K+ greater than Rb+ greater than Cs+ much greater than choline. Chloride and divalent cations did not affect the glutamate-evoked current. 4. Raising the intracellular sodium or glutamate concentrations, or raising the extracellular potassium concentration, reduced the current evoked by external glutamate. The suppressive effect of internal glutamate was larger when the internal sodium concentration was high. 5. Some analogues of glutamate also evoked an inward current. Responses to L-aspartate resembled those to glutamate, but for aspartate the apparent affinity was higher and the voltage dependence of the current was steeper. In the physiological potential range the current evoked by a saturating dose of aspartate was less than that evoked by a saturating dose of glutamate. 6. The uptake blocker threo-3-hydroxy-DL-aspartate (30 microM) reduced the glutamate-evoked current, but also generated a current itself. Dihydrokainate (510 microMs) weakly inhibited the glutamate-evoked current without generating a current itself. 7. The commonly used blockers of glutamate-gated ion channels, 2-amino-5-phosphonovalerate (APV; 100 microMs), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 20 microMs), and kynurenate (1mM) had no effect on the glutamate-evoked current. 8. The voltage dependence, cation dependence and pharmacological profile of the current evoked by excitatory amino acids indicate that it is caused by activation of the high-affinity glutamate uptake carrier. This carrier appears to transport one glutamate anion into the cell, one K+ ion out of the cell, and two or more Na+ ions into the cell, on each carrier cycle. At the inner membrane surface some or all of the transported Na+ dissociates from the carrier after the transported glutamate has dissociated. 9. In addition to glutamate, the uptake carrier can also transport aspartate and threo-3-hydroxy-DL-aspartate, but not dihydrokainate.
Synthesis, Biological Evaluation And QSAR Analysis Of Some New Derivatives Of Ketoprofen And Flurbiprofen (1998)

New derivatives of ketoprofen and flurbiprofen were synthesized by Mannich reaction. The Mannich base amides of ketoprofen and flurbiprofen were synthesized using different amines. These were then evaluated for analgesic, anti-inflammatory, ulcerogenic activities and quantitative structure-activity relationship was analysed. Some of the derivatives were found to be potent with practically no ulcerogenic activity.
디지털 사이니지 표현기술에 따른 콘텐츠 연구 (2012)

Digital signagc is a form of media associated with places. What is significant in the concept of producing signage is to communicate with the public, and find, explore and apply a new method. It is not an invention concept, but a creation work. Also, it is to maximize the visual illusion of a new experience in harmony with design, technology and spatial design, There are three methods of making Trompe-l`oeil the first one is to place background and main objects on flat places, such as screen, wall or tables, with a prop. In the state, it is possible to oxpcrience contents; the second one is to use an input device through recognition of the motions of the human body. In other words, it uses moving gestures that occur in casual conversation and responds to them; and the third one is to float objects in space for display. These days, the method is usually applied. Floating image has the biggest advantage of being less affected by surroundings, although a space is not dark, and of creating independent contents. To become a leading country m the digital signage area, it is required to develop a core technology rather than a technology being sensitive to speed and responses. Also, it is necessary to develop digital signage. which can be used in everyday life, commercially valuable, and needed for customers, through application of contents and software, and to provide the media in an innovative and magical way. If realized so, the digital signage will become a new growth area where innovation ideas, technology, and advertising marketing are merged together.
Computer networking representations for parallel distributed computing algorithms (2005)

Concerns the application of the mean field annealing (MFA) algorithm for combinatorial optimization problems. In particular, discrete optimization problems may be reduced to minimization of a 0-1 Hamiltonian. A significant algorithm for finding optimal solutions to such problems is the simulated annealing (SA) algorithm. It models the degrees of freedom in a problem as if it were a collection of atoms slowly being cooled into a ground state corresponding to the optimal solution to the problem. Although its principle may be based on complex and massive connections between network elements, there may be the bound of connective complexity in the realization. To overcome this problem we might apply the MFA, which is based on a stochastic optimization model. This paper proposes a network computing technique to perform the MFA, and shows how to apply it to the graph partitioning problem.
Evaluation of Gene Therapy as a Treatment for Equine Traumatic Arthritis and Osteoarthritis (2000)

Joint disease in horses and in humans is a significant social and economic problem and continued research and improvements in therapeutics are needed. Because horses have naturally occurring osteoarthritis that is similar to that of humans, the horse was chosen as a species to investigate gene transfer as a potential therapeutic modality for the treatment of osteoarthritis. Using an established model of equine osteoarthritis, the therapeutic effects resulting from overexpression the equine interleukin-1 receptor antagonist gene sequence through adenoviral mediated gene transfer was investigated. The results of the current study showed intraarticular expression of interleukin-1 receptor antagonist to have favorable effects such as an approximately 28 day upregulation of interleukin-1 receptor antagonist protein expression, significant improvement in clinical parameters of pain and disease activity, and beneficial effects in histologic parameters measured from synovial membrane and articular cartilage when compared with nontransduced joints. Based on the significant improvements seen in this work gene transfer of interleukin-1 receptor antagonist is a practical treatment modality for the equine patient and also offers future promise for human patients.
Presence of a Surface-Exposed Loop Facilitates Trypsinization of Particles of Sinsiro Virus, a Genogroup II.3 Norovirus (2007)

Noroviruses (NoVs) are the causative agents of nonbacterial acute gastroenteritis in humans. NoVs that belong to genogroup II (GII) are quite prevalent and prone to undergo recombination, and their three-dimensional structure is not yet known. Protein homology modeling of Sinsiro virus (SV), a member of the GII.3 NoVs, revealed the presence of a surface-exposed 20-amino-acid (aa) insertion in the P2 domain of the capsid protein (CP) relative to the Norwalk virus (NV) CP, which is a well known hot spot for mutations to counter the host immunological response. To further characterize the role of the long insertion in SV, the capsid protein gene was expressed using the recombinant baculovirus system. Trypsinization of the resultant virus-like particles yielded two predominant bands (31.7 and 26.1 kDa) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis. N-terminal sequencing and analysis of the mass spectroscopic data indicated that these fragments correspond to residues 1 to 292 (26.1 kDa) and 307 to 544 (31.7 kDa). In addition, the above data taken together with the comparative modeling studies indicated that the trypsin cleavage sites of the Sinsiro virus CP, Arg292 and Arg307, are located at the beginning of and within the 20-aa insertion in the P2 domain, respectively. This study demonstrates that the presence of the surface-exposed loop in the GII.3 NoVs facilitates the trypsinization of the capsid protein in the assembled form. The SV particles remain intact even after trypsin digestion and retain the suggested receptor binding linear epitope of residues 325 to 334. The above results are distinct from those obtained from the trypsinization studies performed earlier on the NV (GI) and VA387 (GII) viruses, both of which lack the large surface insertion and associated basic residues. These new observations may have implications for host receptor binding, cell entry, and norovirus infection in general.
QT prolongation and proarrhythmia by moxifloxacin: concordance of preclinical models in relation to clinical outcome (2005)

Moxifloxacin, a fluoroquinolone antibiotic associated with QT prolongation, has been recommended as a positive control by regulatory authorities to evaluate the sensitivity of both clinical and preclinical studies to detect small but significant increases in QT interval measurements. In this study, we investigated effects of moxifloxacin on the hERG current in HEK‐293 cells, electrocardiograms in conscious telemetered dogs, and repolarization parameters and arrhythmogenic potentials in the arterially perfused rabbit ventricular wedge model. Moxifloxacin inhibited the hERG current with an IC 50 of 35.7 μ M . In conscious telemetered dogs, moxifloxacin significantly prolonged QTc at 30 and 90 mg kg −1 , with mean serum C max of 8.52 and 22.3 μ g ml −1 , respectively. In the wedge preparation, moxifloxacin produced a concentration‐dependent prolongation of the action potential duration, QT interval, and the time between peak and end of the T wave, an indicator for transmural dispersion of repolarization. Phase 2 early after‐depolarizations were observed in one of five experiments at 30 μ M and five of five experiments at 100 μ M . The arrhythmogenic potential was also concentration‐dependent, and 100 μ M (∼18‐fold above the typical unbound C max exposure in clinical usage) appeared to have a high risk of inducing torsade de pointes (TdP). Our data indicated a good correlation among the concentration–response relationships in the three preclinical models and with the available clinical data. The lack of TdP report by moxifloxacin in patients without other risk factors might be attributable to its well‐behaved pharmacokinetic profile and other dose‐limiting effects. British Journal of Pharmacology (2005) 146 , 792–799. doi: 10.1038/sj.bjp.0706389