gebhart/patrepeval huggingface dataset:
Mutations in the human homologue of the Drosophila segment polarity gene patched (PTCH) in sporadic basal cell carcinomas of the skin and primitive neuroectodermal tumors of the central nervous system. (1997)

The human homologue of the Drosophila segment polarity gene patched (PTCH) has recently been identified as the tumor suppressor gene responsible for the nevoid basal cell carcinoma (BCC) syndrome (H. Hahn et al., Cell, 85: 841-851, 1996; R. L. Johnson et al., Science (Washington DC), 272: 1668-1671, 1996). In addition to multiple BCCs, patients with nevoid BCC syndrome have a predisposition for the development of primitive neuroectodermal tumors (PNETs) of the central nervous system. We have analyzed 9 sporadic BCCs and 37 PNETs for mutation and expression of the PTCH gene. PTCH mutations were found in 3 BCCs (33.3%) and in 5 PNETs (14%), including 1 of 5 cerebral PNETs, 2 of 15 medulloblastomas, and 2 of 17 desmoplastic medulloblastomas. The sequence changes in six of these tumors (four PNETs, two BCCs) were mutations predicted to result in truncated proteins. Missense mutations were detected in one PNET and one BCC each. In addition, novel sequence polymorphisms were found in exon 2, intron 5, intron 10, and intron 14 of PTCH. Reverse transcription-PCR analysis revealed increased PTCH expression levels compared to nonneoplastic brain tissue and normal skin in the majority of PNETs and BCCs investigated. Our data suggest that genetic alterations of PTCH are not only of significance in hereditary and sporadic BCCs but are also involved in the molecular pathogenesis of a subset of sporadic central nervous system PNETs.
JAK-STAT Signal Inhibition Regulates Competition in the <i>Drosophila</i> Testis Stem Cell Niche (2009)

Adult stem cells often reside in local microenvironments, or niches. Although niches can contain multiple types of stem cells, the coordinate regulation of stem cell behavior is poorly understood. In the Drosophila testis, Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling is directly required for maintenance of the resident germline and somatic stem cells. We found that the JAK-STAT signaling target and inhibitor Suppressor of cytokine signaling 36E (SOCS36E) is required for germline stem cell maintenance. SOCS36E suppresses JAK-STAT signaling specifically in the somatic stem cells, preventing them from displacing neighboring germline stem cells in a manner that depends on the adhesion protein integrin. Thus, in niches housing multiple stem cell types, negative feedback loops can modulate signaling, preventing one stem cell population from outcompeting the other.
Repair and Replacement of Small Arteries, Microsuture Technique (1966)

ECO~STRUCTIVE procedures on small arteries have not been as extensive and as successful as those of the larger vessels. Progressive improvements have been nlade in this field in relation to large vessels since the concept of vascular reconstruct ion was introduced two centuries ago. However , the progress has been limited in arteries of small calibre. At the present t ime the methods available for repair and replacement of small arteries are: (1) Gross suture technique; (2) Prosthet ic technique; (3) Adhesive technique ;7 (4) Stapling technique; and (5) Microsuture technique. Among these the microsuture technique is the mos t recent me thod of vascular repair and replacement. This was introduced by Jacobson and Suarez in 19603. Although it was felt t ha t the microsuture technique would extend vascular surgery to m a n y previously inaccessible areas, not much has been wri t ten on the subject. The present s tudy is similar to the one repor ted by Jacobson and Suarez; however, there are a few new observat ions and procedures in this report.
Single-mode fiber-optic directional coupler (1982)

A low-loss compact single-mode fiber optical directional coupler (SMF-ODC) is developed for use in SMF reflectometers or gyroscopes. The SMF-ODC is made of two sapphire ball lenses, a half-mirror, and two ferrules. Two fibers (endfaces are concave) are aligned parallel to each other in the ferrule. The optical and mechanical axes are tilted. Cross-talk light from fibers or lenses is avoided. The SMF-ODC excess loss is 1.5-2.5 dB at the 1.2-1.5-microm wavelength range. The directivity is 53 (51) dB at the 1.3 (1.53)-microm wavelength. The light polarization dependency is < or =0.1 dB. The return loss is > or =43 dB.
Reversible Thermal Tuning of All‐Dielectric Metasurfaces (2017)

All‐dielectric metasurfaces provide a powerful platform for a new generation of flat optical devices, in particular, for applications in telecommunication systems, due to their low losses and high transparency in the infrared. However, active and reversible tuning of such metasurfaces remains a challenge. This study experimentally demonstrates and theoretically justifies a novel scenario of the dynamical reversible tuning of all‐dielectric metasurfaces based on the temperature‐dependent change of the refractive index of silicon. How to design an all‐dielectric metasurface with sharp resonances by achieving interference between magnetic dipole and electric quadrupole modes of constituted nanoparticles arranged in a 2D lattice is shown. Thermal tuning of these resonances can cause drastic but reciprocal changes in the directional scattering of the metasurface in a spectral window of 75 nm. This change can result in a 50‐fold enhancement of the radiation directionality. This type of reversible tuning can play a significant role in novel flat optical devices including the metalenses and metaholograms.
Register binding and port assignment for multiplexer optimization (2004)

Data path connection elements, such as multiplexers, consume a significant amount of area on a VLSI chip, especially for FPGA designs. Multiplexer optimization is a difficult problem because both register binding and port assignment to reduce total multiplexer connectivity during high-level synthesis are NP-complete problems. In this paper, we first formulate a k-cofamily-based register binding algorithm targeting the multiplexer optimization problem. We then further reduce the multiplexer width through an efficient port assignment algorithm. Experimental results show that we are 44% better overall than the left-edge register binding algorithm on the total usage of multiplexer inputs and 7% better than a bipartite graph-based algorithm. For large designs, we are able to achieve significantly better results consistently. After technology mapping, placement and routing for an FPGA architecture, it shows considerably positive impacts on chip area, delay and power consumption.
Effect of lecithin and starch on alginate-encapsulated probiotic bacteria (2010)

The effect of lecithin and starch on viability of alginate encapsulated probiotics was determined at different temperatures. Probiotic organisms (1% v/v>10Log CFU ml(-1)) were encapsulated using alginate (2% w/v), gelatinized starches (2% w/v) and lecithin (0-4% w/v) and stored in sealed containers at 4, 23 and 37 degrees C (to simulate shelf storage conditions). Incorporation of lecithin improved the entrapment efficiency (p < 0.05) and the viability of encapsulated bacteria (p = 0.02). Encapsulated Lactobacillus, Bifidobacterium species and Lactococcus lactis in lecithin containing freeze-dried beads had good survival stability (above 6Log CFU ml(-1)) at 23 degrees C for 12 weeks. The bacteria in the beads showed 6Log survival by the end of 2 weeks at 37 degrees C. Encapsulated L. casei in the alginate beads containing lecithin were also more stable in the yoghurt than the beads without lecithin. SEM analysis of the beads showed an irregular surface for the beads without lecithin.
Effects of Ovariectomy and Dehydroepiandrosterone (DHEA) on Vaginal Wall Thickness and Innervation (2012)

The relatively potent stimulatory effect of DHEA on intravaginal nerve fiber density provides a possible explanation for the beneficial effects of intravaginal DHEA on sexual dysfunction observed in postmenopausal women.
Multiple-Dose Arecoline Infusions in Alzheimer's Disease (1988)

Twelve patients with dementia of the Alzheimer type received two-hour infusions of placebo and the muscarinic cholinergic agonist arecoline hydrobromide at rates of 1, 2, and 4 mg/h in a double-blind, randomized fashion. These infusions resulted in dose-dependent physiologic and neuroendocrine effects consistent with central cholinergic stimulation. Infusions were generally well tolerated. No statistically significant improvement in performance on most cognitive tasks assessing knowledge memory and episodic learning and memory was observed at any dose, although marginal improvement in picture recognition ability and in ratings of word-finding were observed at the lower doses. Psychomotor activation and slightly improved affect were reliably observed at the lower doses, whereas increasing psychomotor retardation was observed at the highest dose. The data support a role for central cholinergic modulation of some aspects of cognition, behavior, and affect in this population. The apparent greater behavioral sensitivity of patients with Alzheimer's disease in comparison with subject populations previously studied, as well as the altered dose responsiveness, merit further study in relationship to normal aging.
[A special type of mechanical acne: fiddler's neck dermatitis]. (1984)

Seven cases of trauma-induced dermatitis of the neck, are described. They were all the result of violin playing (Fiddler's neck). The histological, clinical and the therapeutic characteristics of our cases are similar to those of mechanical acne.
Assessment of gliosis around moveable implants in the brain (2009)

Repositioning microelectrodes post-implantation is emerging as a promising approach to achieve long-term reliability in single neuronal recordings. The main goal of this study was to (a) assess glial reaction in response to movement of microelectrodes in the brain post-implantation and (b) determine an optimal window of time post-implantation when movement of microelectrodes within the brain would result in minimal glial reaction. Eleven Sprague-Dawley rats were implanted with two microelectrodes each that could be moved in vivo post-implantation. Three cohorts were investigated: (1) microelectrode moved at day 2 (n = 4 animals), (2) microelectrode moved at day 14 (n = 5 animals) and (3) microelectrode moved at day 28 (n = 2 animals). Histological evaluation was performed in cohorts 1-3 at four-week post-movement (30 days, 42 days and 56 days post-implantation, respectively). In addition, five control animals were implanted with microelectrodes that were not moved. Control animals were implanted for (1) 30 days (n = 1), (2) 42 days (n = 2) and (3) 56 days (n = 2) prior to histological evaluation. Quantitative assessment of glial fibrillary acidic protein (GFAP) around the tip of the microelectrodes demonstrated that GFAP levels were similar around microelectrodes moved at day 2 when compared to the 30-day controls. However, GFAP expression levels around microelectrode tips that moved at day 14 and day 28 were significantly less than those around control microelectrodes implanted for 42 and 56 days, respectively. Therefore, we conclude that moving microelectrodes after implantation is a viable strategy that does not result in any additional damage to brain tissue. Further, moving the microelectrode downwards after 14 days of implantation may actually reduce the levels of GFAP expression around the tips of the microelectrodes in the long term.
Construction of tryptophan-producing recombinant strains of Brevibacterium lactofermentum using the engineered trp operons. (1988)

Brevibacterium lactofermentum is an industrially important Gram-positive bacterium used for the production of such amino acids as glutamic acid, lysine and threonine. Wehave directed our attention to breeding the tryptophan producer of this microorganism, because tryptophan is an important ingredient in medicines and animal feeds as an essential aminoacid.
The thrombopoietin/MPL axis is activated in the Gata1low mouse model of myelofibrosis and is associated with a defective RPS14 signature (2017)

Myelofibrosis (MF) is characterized by hyperactivation of thrombopoietin (TPO) signaling, which induces a RPS14 deficiency that de-regulates GATA1 in megakaryocytes by hampering its mRNA translation. As mice carrying the hypomorphic Gata1<sup>low</sup> mutation, which reduces the levels of Gata1 mRNA in megakaryocytes, develop MF, we investigated whether the TPO axis is hyperactive in this model. Gata1<sup>low</sup> mice contained two times more Tpo mRNA in liver and TPO in plasma than wild-type littermates. Furthermore, Gata1<sup>low</sup> LSKs expressed levels of Mpl mRNA (five times greater than normal) and protein (two times lower than normal) similar to those expressed by LSKs from TPO-treated wild-type mice. Gata1<sup>low</sup> marrow and spleen contained more JAK2/STAT5 than wild-type tissues, an indication that these organs were reach of TPO-responsive cells. Moreover, treatment of Gata1<sup>low</sup> mice with the JAK inhibitor ruxolitinib reduced their splenomegaly. Also in Gata1<sup>low</sup> mice activation of the TPO/MPL axis was associated with a RSP14 deficiency and a discordant microarray ribosome signature (reduced RPS24, RPS26 and SBDS expression). Finally, electron microscopy revealed that Gata1<sup>low</sup> megakaryocytes contained poorly developed endoplasmic reticulum with rare polysomes. In summary, Gata1<sup>low</sup> mice are a bona fide model of MF, which recapitulates the hyperactivation of the TPO/MPL/JAK2 axis observed in megakaryocytes from myelofibrotic patients.
Fatty Acid Receptor Gpr40 Mediates Neuromicrovascular Degeneration Induced by Transarachidonic Acids in Rodents (2013)

GPR40 emerges as the first identified G protein-coupled receptor conveying actions of nonenzymatically generated nitro-oxidative products, specifically TAAs, and is involved in (neonatal) HI encephalopathy.
Combined QF‐PCR and MLPA molecular analysis of miscarriage products: an efficient and robust alternative to karyotype analysis (2009)

This molecular approach is less labour-intensive, allows a higher sample throughput and has a higher success rate than karyotype analysis; it is therefore an efficient and cost-effective diagnostic testing strategy for miscarriage products.
Establishment of a transient expression system for<i>Dictyostelium discoideum</i> (1988)

We have established a rapid and sensitive transient expression system for Dictyostelium discoideum. We constructed a gene fusion containing the promoter from the Dictyostelium Actin 15 gene fused to the firefly luciferase gene. The enzymatic activity of this gene fusion, expressed at very high levels in stable transformants, was measured to determine optimum conditions for transient expression using electroporation to introduce the DNA into cells. With these conditions, we show that a luciferase gene fusion driven by a prestalk, cell-type specific promoter from the pst-cathepsin gene expresses luciferase at the appropriate developmental stage. In addition, we present results suggesting that the system will be useful for expressing genes in non-axenic cell lines. Finally, we observe that electroporation is more efficient for obtaining stable transformations than the standard calcium phosphate procedure using extrachromosomally replicating shuttle vectors but less efficient for vectors that integrate into the Dictyostelium chromosomes.
A simulation study of harmonics regeneration in noise reduction for electric and acoustic stimulation (2010)

Recent research results show that combined electric and acoustic stimulation (EAS) significantly improves speech recognition in noise, and it is generally established that access to the improved F0 representation of target speech, along with the glimpse cues, provide the EAS benefits. Under noisy listening conditions, noise signals degrade these important cues by introducing undesired temporal-frequency components and corrupting harmonics structure. In this study, the potential of combining noise reduction and harmonics regeneration techniques was investigated to further improve speech intelligibility in noise by providing improved beneficial cues for EAS. Three hypotheses were tested: (1) noise reduction methods can improve speech intelligibility in noise for EAS; (2) harmonics regeneration after noise reduction can further improve speech intelligibility in noise for EAS; and (3) harmonics sideband constraints in frequency domain (or equivalently, amplitude modulation in temporal domain), even deterministic ones, can provide additional benefits. Test results demonstrate that combining noise reduction and harmonics regeneration can significantly improve speech recognition in noise for EAS, and it is also beneficial to preserve the harmonics sidebands under adverse listening conditions. This finding warrants further work into the development of algorithms that regenerate harmonics and the related sidebands for EAS processing under noisy conditions.
Roles of ethylene and cytokinins in development of defense responses in Triticum aestivum plants infected with Septoria nodorum (2016)

Effects of ethephon (2-chloroethylphosphonic acid, ET), which is a producer of ethylene, and 1-methylcyclopropene (1-MCP), which inhibits ethylene binding with the corresponding receptors, on defense responses caused by the causal agent of leaf blotch (Septoria nodorum Berk.) in leaves of soft spring wheat (Triticum aestivum L.) of cultivars contrast in the resistance to the pathogen were studied. After treatment with 1-MCP, an induction of wheat resistance to the disease, more prominent in the susceptible cv. Kazakhstanskaya 10 than in the resistant cv. Omskaya 35, was found. The rise in the resistance was accompanied by rise in zeatin content in leaves, enhanced generation of hydrogen peroxide (most likely, due to the decreased catalase activity and increased peroxidase activity), and accumulation of transcripts of marker genes of the salicylate signaling pathway (PR-1 and PR-2). On the contrary, in ET-treated plants, all the studied defense responses were inhibited, and the pathogen developed more intensively. The effect of ethylene on zeatin distribution in infected wheat leaves of the susceptible cv. Kazakhstanskaya 10 was also found. In the 1-MCP-treated wheat leaves, cytokinins were localized in mesophyll cells and cell walls. In the ET-treated leaves, cell walls were free of zeatin, and the hormone concentrated in developing hyphae of the pathogen. The results allow for the hypothesis that wheat plant resistance is controlled by antagonistic interaction of signaling pathways of salicylic acid and ethylene with participation of cytokinins.
Elimination of Self-Reactive T Cells in the Thymus: A Timeline for Negative Selection (2013)

The elimination of autoreactive T cells occurs via thymocyte apoptosis and removal by thymic phagocytes, but the sequence of events in vivo, and the relationship between thymocyte death and phagocytic clearance, are unknown. Here we address these questions by following a synchronized cohort of thymocytes undergoing negative selection within a three-dimensional thymic tissue environment, from the initial encounter with a negative selecting ligand to thymocyte death and clearance. Encounter with cognate peptide-MHC complexes results in rapid calcium flux and migratory arrest in auto-reactive thymocytes over a broad range of peptide concentrations, followed by a lag period in which gene expression changes occurred, but there was little sign of thymocyte death. Caspase 3 activation and thymocyte loss were first detectable at 2 and 3 hours, respectively, and entry of individual thymocytes into the death program occurred asynchronously over the next 10 hours. Two-photon time-lapse imaging revealed that thymocyte death and phagocytosis occurred simultaneously, often with thymocytes engulfed prior to changes in chromatin and membrane permeability. Our data provide a timeline for negative selection and reveal close coupling between cell death and clearance in the thymus.
‘The Popish Army of the North’: Anti-Catholicism and Parliamentarian Allegiance in Civil War Yorkshire, 1642–46 (2000)

By the time of the outbreak of the Civil Wars, may educated British Protestants considered Roman Catholicism to be an anti-religion; indeed, the Cambridge divine William Fulke went so far as to equate it with devil worship. Wealthy and powerful English Catholics attracted extreme hostility in moments of political crisis throughout the early modern period, but in 1642, fear of Roman Catholicism was even used to legitimate the terrible act of rebellion. Keith Lindley has emphasized the civil war neutrality of English Catholics, while many current historians, nervous of displays of religious prejudice, have portrayed the anti-Catholic fears of parliamentarians as cynical propaganda. Michael Finlayson has condemned anti-Catholicism as ‘irrational paranoia’, to be compared with anti-Semitism, which might, had it not been for the growth of liberal traditions in nineteenth-century England, have led to some sort of ‘Final Solution’.
Therapeutic potential of K<sub>Ca</sub>3.1 blockers: recent advances and promising trends (2010)

The Ca(2+)-activated K(+) channel K(Ca)3.1 regulates membrane potential and calcium signaling in erythrocytes, activated T and B cells, macrophages, microglia, vascular endothelium, epithelia, and proliferating vascular smooth muscle cells and fibroblasts. K(Ca)3.1 has therefore been suggested as a potential therapeutic target for diseases such as sickle cell anemia, asthma, coronary restenosis after angioplasty, atherosclerosis, kidney fibrosis and autoimmunity, where activation and excessive proliferation of one or more of these cell types is involved in the pathology. This article will review the physiology and pharmacology of K(Ca)3.1 and critically examine the available preclinical and clinical data validating K(Ca)3.1 as a therapeutic target.
Formation of Bridging Alkene and Conjugated Dialkenes Exclusively Generated from Alkynes on the [3,3‘-Co(1,2-C<sub>2</sub>B<sub>9</sub>H<sub>11</sub>)<sub>2</sub>]<sup>-</sup> Platform. The Unique Hydroboration Role of [3,3‘-Co(1,2-C<sub>2</sub>B<sub>9</sub>H<sub>11</sub>)<sub>2</sub>]<sup>-</sup> (2003)

The unprecedented metal-mediated transformation of an alkyne into a B,B' bridging alkene is reported. Also, the unprecedented synthesis of a conjugated dialkene derivative of [3,3'-Co(1,2-C2B9H11)2]- generated only from an alkyne, contrary to the usual case where an alkyne and an alkene are needed, is described. This has been possible through the singular capacity of a B-H to produce hydroboration.