gebhart/patrepeval huggingface dataset:
Inhibition of human epithelial ovarian cancer cellgrowth in vitro by agonistic and antagonistic analogues of luteinizinghormone-releasing hormone. (1994)

In this study, we investigated the effects of luteinizing hormone-releasing hormone (LH-RH) agonist [D-Trp6]LH-RH, LH-RH antagonist [Ac-D-Nal(2)1,D-Phe(pCl)2,D-Pal(3)3,D-Cit6,D-Ala10]LH-RH (SB-75), and estradiol on the growth of human epithelial ovarian cancer cell line OV-1063. Cells were cultured under estrogen-deprived conditions. Estradiol inhibited cell proliferation, as measured by cell number at 10(-9)-10(-7) M and [3H]thymidine incorporation into DNA at 10(-13)-10(-8) M. Both LH-RH analogs inhibited cell growth dose dependently in the range 10(-8)-10(-5) M, but SB-75 induced a greater growth inhibition than [D-Trp6]LH-RH. In OV-1063 cells, 125I-labeled [D-Trp6]LH-RH was bound to one class of specific, saturable binding sites with high affinity (Kd = 1.4 +/- 0.3 nM) and low capacity (4000 binding sites per cell). 125I-labeled [D-Trp6]LH-RH could be displaced by unlabeled [D-Trp6]LH-RH and SB-75, suggesting that both analogs are bound to the same receptor on OV-1063 cells. Ligand binding was dependent on time and temperature. Receptor internalization assay showed that the ligand-receptor complex was internalized at 37 degrees C, which indicates the presence of biologically active LH-RH receptors on OV-1063 cells. These results suggest that estradiol and LH-RH analogs can suppress the growth of OV-1063 human epithelial ovarian cancer cells by a direct action and that the inhibitory effect of LH-RH analogs is mediated through the high-affinity LH-RH receptors.
Abstract P6-04-04: Hypoxia Is Associated with Somatic Loss of BRCA1 Protein and Pathway Activity in Triple Negative Breast Cancer (2010)

Abstract Background: Triple negative breast cancer (TNBC) has loss of BRCA1 activity either through germline mutation, epigenetic modification or negative transcriptional regulators. Yale investigators have demonstrated that tumor hypoxia leads to loss of critical DNA repair activities, including BRCA1, RAD-51 and γ-H2AX (Bindra RS, et al. Cancer Res 2005;65(24): 11597-604). This study was designed to explore the relationship of BRCA1 loss and hypoxia using Carbonic Anhydrase IX (CA-IX), a downstream target of hypoxia-induced factor 1a (HIF-1a), an accepted surrogate biomarker for tumor hypoxia, in TNBC. Methods: Two cohorts of breast tumors from Yale archival materials were studied. The first consisted of 660, unselected breast tumors, the second a cohort of 130 TNBC, both with long term clinical followup. Automated Quantitative Analysis (AQUA) was used to detect the intensity of BRCA1 and CAIX within specific subcellular compartments. CA-IX antibody M75 was provided by the J Zavada lab (1:10000), BRCA1 monoclonal antibody M110 (Ab-1) was purchased from Calbiochem (1:1000). The 130 TNBC cohort was also subjected to whole genome expression analysis. In brief, tissue core biopsies from tumor blocks were subjected to nucleic acid extraction using RecoverAll Total Nucleic Acid Isolation kit (Applied Biosystems) and 600ng total RNA were processed by the Keck Microarray Facility for the Illumina DASL platform. Statistical analysis of gene expression data was carried out in Bioconductor/R software. A set of relevant signatures was selected by enrichment analysis of modules identified by principal component analysis. Signature scores were computed as Pearson correlation between the signature vector of gene contributions and each sample's expression profile for these genes. Results: In the cohort of 660 specimens, 22 were found to have high CA-IX expression score (above cut-off value of 10). Cut-off was based on known positive cell lines and corresponding AQUA scores, as well as visual confirmation of positivity. A negative correlation (Rho=-0.6, p=0.0165) of BRCA1 nuclear protein with CA-IX level was found and this data was reproducible on a duplicate array. Of note, 14 out of 22 hypoxic breast tumors were from triple negative breast cancers (TNBC) (p=0.0034). In the triple negative cohort, CAIX staining was positive in 20 % of cases (n=15/75) and in these samples it was associated with the 2002 van ‘t Veer BRCA1 mutant signature (Rho=0.51). Positive CAIX staining was also associated with worse outcome (p=0.046) as was CAIX mRNA expression (p=0.02). Conclusions: In human breast tumors BRCA1 nuclear expression is negatively correlated with tumor hypoxia, measured by CAIX. A subset of TNBC has higher levels of hypoxia and BRCA1 signatures. This data suggests that CAIX may be a useful biomarker for BRCA1 loss, and possibly for response to PARP inhibitor therapy. This will be evaluated in our recently completed trial of BSI-201+Irinotecan. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P6-04-04.
Carbon nanotube effects on electroluminescence and photovoltaic response in conjugated polymers (2005)

This letter reports the experimental results of enhanced electroluminescence (EL) and photovoltaic (PV) response upon doping single-wall carbon nanotubes (SWNTs) into conjugated polymer poly[2-methoxy-5-(2′-ethylhexyloxy)-1, 4-phenylenevinylene] (MEHPPV) based on single-layer light-emitting diodes. We found that the dispersed SWNTs result in two processes: charge transport and exciton dissociation at the tube-chain interface in the SWNT/polymer composites. The detailed EL and PV studies indicate that low SWNT doping concentrations mainly improve the bipolar charge injection, leading to enhanced both reverse and forward EL with reduced threshold voltage. As the SWNT doping concentration continues to increase, the interfacial exciton dissociation becomes dominated, giving rise to an increased PV response. This SWNT concentration-dependent charge transport and exciton dissociation present a pathway to individually address the dual EL and PV functionalities of SWNT-doped polymer composites by controlling the doping level of the SWNTs.
Continuation of Statin Therapy in Patients with Presumed Infection: A Randomized Controlled Trial (2010)

This study does not support a beneficial role of continuing preexisting statin therapy on sepsis and inflammatory parameters. Cessation of established statin therapy was not associated with an inflammatory rebound. Clinical trial registered at the Australian New Zealand Clinical Trials Registry (ACTRN 12605000756628).
Identification and Characterization of a Novel Diterpene Gene Cluster in Aspergillus nidulans (2012)

Fungal secondary metabolites are a rich source of medically useful compounds due to their pharmaceutical and toxic properties. Sequencing of fungal genomes has revealed numerous secondary metabolite gene clusters, yet products of many of these biosynthetic pathways are unknown since the expression of the clustered genes usually remains silent in normal laboratory conditions. Therefore, to discover new metabolites, it is important to find ways to induce the expression of genes in these otherwise silent biosynthetic clusters. We discovered a novel secondary metabolite in Aspergillus nidulans by predicting a biosynthetic gene cluster with genomic mining. A Zn(II)(2)Cys(6)-type transcription factor, PbcR, was identified, and its role as a pathway-specific activator for the predicted gene cluster was demonstrated. Overexpression of pbcR upregulated the transcription of seven genes in the identified cluster and led to the production of a diterpene compound, which was characterized with GC/MS as ent-pimara-8(14),15-diene. A change in morphology was also observed in the strains overexpressing pbcR. The activation of a cryptic gene cluster by overexpression of its putative Zn(II)(2)Cys(6)-type transcription factor led to discovery of a novel secondary metabolite in Aspergillus nidulans. Quantitative real-time PCR and DNA array analysis allowed us to predict the borders of the biosynthetic gene cluster. Furthermore, we identified a novel fungal pimaradiene cyclase gene as well as genes encoding 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase and a geranylgeranyl pyrophosphate (GGPP) synthase. None of these genes have been previously implicated in the biosynthesis of terpenes in Aspergillus nidulans. These results identify the first Aspergillus nidulans diterpene gene cluster and suggest a biosynthetic pathway for ent-pimara-8(14),15-diene.
CMOS fully-differential bandpass ΣΔmodulator usingswitched-current circuits (1996)

The authors present a fourth-order bandpass ΣΔ switched-current modulator IC in 0.8 µm CMOS single-poly technology. It is the first reported integrated circuit realisation of a bandpass ΣΔ modulator using switched-current circuits. Its architecture is obtained by applying a lowpass to bandpass transformation (z-1 → – z-2) to a second-order lowpass modulator. It has been realised using fully-differential circuitry with common-mode feedback. Measurements show 8 bit dynamic range up to 5 MHz clock frequency.
Increased lymphocyte beta‐adrenergic receptor density in progressive multiple sclerosis is specific for the CD8+, CD28− suppressor cell (1991)

Beta-adrenergic receptor density on T cells from healthy humans is greatest on suppressor cells (CD8+, CD28-) and the effect of catecholamines, secreted by the sympathetic nervous system, predominates on this subset. The sympathetic skin response, a measure of sympathetic nervous system function, is absent in most patients with chronic progressive multiple sclerosis (MS). We measured beta-adrenergic receptor density on suppressor cells, cytotoxic cells, and monocytes from patients with chronic progressive MS and healthy control subjects. Control receptor density on suppressor cells was 2.8 +/- 0.3 fmol/10(6) cells versus a density of 5.1 +/- 0.7 fmol/10(6) cells for patients. Cytotoxic cell (CD8+, CD28+) receptor density was 1.4 +/- 0.4 fmol/10(6) cells in control subjects and 0.9 +/- 0.3 fmol/10(6) cells in the patients. Monocytes displayed beta-adrenergic receptor densities of 2.6 +/- 0.4 fmol/10(6) cells in normal individuals and 2.7 +/- 0.4 fmol/10(6) cells in the patient group. CD8 lymphocyte beta-adrenergic receptor densities in patients with relapsing-remitting and those with stable MS were not different from control values, yet were significantly less than the values for patients with chronic progressive MS. We find that mononuclear cells from healthy control subjects and patients with chronic progressive MS proliferate in response to 200 units/ml of recombinant human interleukin-2 (IL-2) similarly. However, IL-2 treatment increased beta-adrenergic receptor density on normal mononuclear cells, but failed to increase it on mononuclear cells from patients with chronic progressive MS.(ABSTRACT TRUNCATED AT 250 WORDS)
Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips (2016)

We demonstrate an acoustic platform for micro-vortexing in disposable polymer microfluidic chips with small-volume (20 μl) reaction chambers. The described method is demonstrated for a variety of standard vortexing functions, including mixing of fluids, re-suspension of a pellet of magnetic beads collected by a magnet placed on the chip, and lysis of cells for DNA extraction. The device is based on a modified Langevin-type ultrasonic transducer with an exponential horn for efficient coupling into the microfluidic chip, which is actuated by a low-cost fixed-frequency electronic driver board. The transducer is optimized by numerical modelling, and different demonstrated vortexing functions are realized by actuating the transducer for varying times; from fractions of a second for fluid mixing, to half a minute for cell lysis and DNA extraction. The platform can be operated during 1 min below physiological temperatures with the help of a PC fan, a Peltier element and an aluminum heat sink acting as the chip holder. As a proof of principle for sample preparation applications, we demonstrate on-chip cell lysis and DNA extraction within 25 s. The method is of interest for automating and chip-integrating sample preparation procedures in various biological assays.
[Prediction of the parameters of olfactory activity of ambient air pollutants]. (2009)

The paper presents rapid experimental and calculating methods for predicting the parameters of the olfactory activity of ambient air pollutants. The experimentally established and designed thresholds of the odor of benzene derivatives were compared, which indicated the reliability of the methods. These procedures were used in the studies by this and other investigators to establish the OBUV of more than 30 substances and to design experiments determining the ambient air thresholds of the odor of the substances.
High-performance metal/silicide antifuse (for CMOS technology) (1992)

A low-programmed-resistance low-thermal-budget, high-performance metal/silicide antifuse is reported. The programmed ON-State resistance of the metal/silicide antifuse is around 60 Omega , which is a factor of 10 less than that of Si-based antifuses (poly/n/sup +/ and poly/poly). Metal/silicide antifuses also eliminate the nonlinear ON-state resistance seen in Si-based antifuses. Programming of the antifuse can be done in 2 ms at 14 V, which is comparable to Si-based antifuses. Both ON- and OFF-state reliability of the metal/silicide antifuse are shown to be satisfactory.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation (2011)

Chordates undergo a characteristic morphogenetic process during neurulation to form a dorsal hollow neural tube. Neurulation begins with the formation of the neural plate and ends when the left epidermis and right epidermis overlying the neural tube fuse to close the neural fold. During these processes, mitosis and the various morphogenetic movements need to be coordinated. In this study, we investigated the epidermal cell cycle in Ciona intestinalis embryos in vivo using a fluorescent ubiquitination-based cell cycle indicator (Fucci). Epidermal cells of Ciona undergo 11 divisions as the embryos progress from fertilization to the tadpole larval stage. We detected a long G2 phase between the tenth and eleventh cell divisions, during which fusion of the left and right epidermis occurred. Characteristic cell shape change and actin filament regulation were observed during the G2 phase. CDC25 is probably a key regulator of the cell cycle progression of epidermal cells. Artificially shortening this G2 phase by overexpressing CDC25 caused precocious cell division before or during neural tube closure, thereby disrupting the characteristic morphogenetic movement. Delaying the precocious cell division by prolonging the S phase with aphidicolin ameliorated the effects of CDC25. These results suggest that the long interphase during the eleventh epidermal cell cycle is required for neurulation.
Guest Editors' Introduction: Confronting the Hardware Trustworthiness Problem (2010)

The emergence of a globalized, horizontal semiconductor business model raises concerns involving the security and trust of the information systems on which modern society is increasingly reliant for mission-critical functionality. Traditionally, hardware-based security techniques and countermeasures modify hardware to prevent attacks and to protect IP blocks or secret keys. Some of the emerging types of attacks covered by this special issue are fundamentally different: the attacker is assumed to maliciously alter the design before or during fabrication.
Neuroinflammation Mechanisms and Phytotherapeutic Intervention: A Systematic Review (2020)

Neuroinflammation is indicated in the pathogenesis of several acute and chronic neurological disorders. Acute lesions in the brain parenchyma induce intense and highly complex neuroinflammatory reactions with similar mechanisms among various disease prototypes. Microglial cells in the CNS sense tissue damage and initiate inflammatory responses. The cellular and humoral constituents of the neuroinflammatory reaction to brain injury contribute significantly to secondary brain damage and neurodegeneration. Inflammatory cascades such as proinflammatory cytokines from invading leukocytes and direct cell-mediated cytotoxicity between lymphocytes and neurons are known to cause "collateral damage" in models of acute brain injury. In addition to degeneration and neuronal cell loss, there are secondary inflammatory mechanisms that modulate neuronal activity and affect neuroinflammation which can even be detected at the behavioral level. Hence, several of health conditions result from these pathogenetic conditions which are underlined by progressive neuronal function loss due to chronic inflammation and oxidative stress. In the first part of this Review, we discuss critical neuroinflammatory mediators and their pathways in detail. In the second part, we review the phytochemicals which are considered as potential therapeutic molecules for treating neurodegenerative diseases with an inflammatory component.
Subsonic aerodynamics and performance of a smart vortex generator system (1996)

A new system for active flow control using smart vortex generators (SVG) is presented. Increments in C/max from modern vortex generators (VGs) are determined through wind-tunnel testing on a two-dimensional wing section. Using an optimized VG configuration, a system was built with 1) a shear-flow sensor that detected the onset and depth of stall, 2) a series of shape-memory-alloy VGs, and 3) a lift-todrag (L/D) maximizing controller. The system demonstrated a 14% increase in C /inax, a 2.7-deg rise in a,*,,, a 42% jump in L/D through the stall, and a low a C M penalty of less than 0.1%. Further testing demonstrated that the system consumed only 9.2 W of power, responded hi less than 0.8 s, and was capable of unstalling an airfoil that had exceeded o^,, by up to 3 deg.
Fischer-Tropsch Fuels: Why Are They of Interest to the United States Military? (2005)

<div class="htmlview paragraph">Synthetic fuels can be manufactured through a process known as Fischer-Tropsch (FT) synthesis. The FT process converts synthetic gas, composed of primarily carbon monoxide and hydrogen, derived from various feedstocks (e.g. natural gas, coal, biomass or petroleum coke) into synthetic hydrocarbons. These hydrocarbons can then be further processed into various FT hydrocarbon products, including diesel fuels or jet fuels. The military has several reasons for their interest in FT fuels. This paper explores these reasons, including the emerging global FT industry, the technologies being investigated for power and propulsion systems, the environmental benefits, and the potential for enhanced strategic mobility and energy security.</div>
CURRÍCULOS ESTRUCTURADOS EN LA LÓGICA Y EL MÉTODO DE LA INGENIERÍA (2005)

En una sociedad sujeta a la incertidumbre y a la dinamica del cambio permanente, se precisa de un proceso de formacion integral de seres humanos y profesionales que les permita resolver con independencia y creatividad los problemas mas generales y frecuentes de su profesion y participar activamente en el desarrollo economico y social del pais. Para lograrlo, la Escuela de Ingenieria de Antioquia (EIA) ha asumido el reto de perfeccionar los curriculos de sus programas academicos con criterios de pertinencia, calidad academica y racionalidad, tanto en la duracion como en las actividades pedagogicas, complementadas con el autoaprendizaje, de acuerdo con las tendencias nacionales e internacionales y coherentes con las politicas gubernamentales y el proyecto institucional. En este articulo se presenta la propuesta curricular del programa de ingenieria civil, su estructura disciplinar minima esencial, asi como su articulacion logica, regidas por objetivos y disenadas en correspondencia con la aplicacion del metodo de la ingenieria. Abstract: In a society subjected to the uncertainty and the dynamics of permanent change, it is necessary to have an integral educational process for the students: both as professionals and human beings. This will allow students to solve with independency and creativity the most general and frequent problems of their profession, as well as to participate actively in the economical and social development of their country. To achieve this, the Antioquia School of Engineering (Escuela de Ingenieria de Antioquia) has assumed the challenge of enhancing their academic programs curricula with criteria such as pertinence, academic quality, and rationality. Those criteria take into account national and international tendencies for pedagogical activities, duration, and special emphasis in self-learning. This article shows the proposed curriculum for the Civil Engineering program, its minimal and essential disciplinary structure, as its logical articulation, guided by objectives and designed in correspondence with the application of the Engineering method.
The Nontoxic CTA1‐DD Adjuvant Enhances Protective Immunity Against <i>Helicobacter pylori</i> Infection Following Mucosal Immunization (2006)

Safe and efficacious adjuvants are much needed to facilitate the development of mucosal vaccines. Here, we have asked whether our nontoxic vaccine adjuvant, CTA1-DD, can enhance protective immunity against Helicobacter pylori infection. Intranasal immunizations with H. pylori lysate together with CTA1-DD-adjuvant induced significant protection in C57Bl/6 mice, almost as strong as similar immunizations using cholera toxin (CT)-adjuvant. Protection remained strong even at 8 weeks postchallenge and the bacterial colonization was reduced by 20-fold compared to lysate-immunized controls. Although CTA1-DD was designed to bind to B cells, microMT mice developed significant, but lower, level of protection following immunization. Intranasal immunizations with CT adjuvant in C57Bl/6 mice resulted in the development of severe postimmunization gastritis at 2 and 8 weeks postchallenge, whereas the degree of gastritis was substantially lower in the CTA1-DD-immunized mice. Protection induced by both CTA1-DD- and CT adjuvant was associated with a strong local infiltration of CD4(+) T cells in the gastric mucosa, and recall responses to specific Ag elicited substantial IFN-gamma production, indicating Th1-dominance. These findings clearly demonstrate that CTA1-DD adjuvant is a promising candidate to be further exploited in the development of a mucosal vaccine against H. pylori infection.
Bone and Development (2010)

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