gebhart/patrepeval huggingface dataset:
Automatic Tuning Techniques for Single-Sideband Equipment (1956)

Methods of automatically positioning tuned circuits for rapid frequency changes are discussed. Equipment ranging from low level single-sideband exciter-receivers to high power linear rf amplifiers are considered with attention given to the correlation of positioning accuracy, tuning change time, and ultimate cost of equipment. The merits and limitations of mechanical positioning, follow-up potentiometer servo systems, and phase servo systems, are noted. Special emphasis is placed upon the adjustment of tank circuits in linear power amplifiers. The problem of obtaining phase information for the servo systems is presented and several improved circuits for tuning and loading detection are explained. The paper is concluded with a general discussion of gain control, sequencing of control circuits, and protection of tubes during tuning cycles.
The Effect of Viscosity on Near-Wellbore Tortuosity and Premature Screenouts (1994)

Abstract The success of most fracture treatments is primarily dependent on the ability to evaluate the characteristics and critical mechanisms that control how a formation hydraulically fractures. By developing an understanding of the mechanisms, it is possible to make the necessary improvements to ensure optimum proppant placement and therefore maximize economic results. This discussion presents a simple, low cost method for improving the overall fracture geometry and reducing the risk of premature screenouts. Dramatic improvements in near-wellbore tortuosity and improvements in proppant placement can be achieved by maximizing the viscosity of fluid that is used to initiate the fracture and carry proppant through the near-wellbore region. Using the approaches presented, it has been possible to eliminate premature screenouts and improve the overall proppant placement in many different environments.
Duration of Suppression of Adrenal Steroids after Glucocorticoid Administration (2010)

<p/> <p>Hydrocortisone has long been the treatment of choice for congenital adrenal hyperplasia (CAH). However, treatment with this medication remains problematic. Patients with 21-hydroxylase deficiency CAH have significant diurnal variation in the secretion of 17-hydroxyprogesterone (17OHP). When considering treatment strategies, this variation must be considered along with the pharmacokinetic and pharmacodynamic properties of exogenous glucocorticoids. Orally administered hydrocortisone is highly bioavailable, but it has a short time to maximum concentration (<inline-formula><graphic file="1687-9856-2010-712549-i1.gif"/></inline-formula>) and half life (<inline-formula><graphic file="1687-9856-2010-712549-i2.gif"/></inline-formula>). While prednisone has a somewhat longer <inline-formula><graphic file="1687-9856-2010-712549-i3.gif"/></inline-formula> and <inline-formula><graphic file="1687-9856-2010-712549-i4.gif"/></inline-formula>, they remain relatively short. There have been several studies of the pharmacodynamics of hydrocortisone. We present data indicating that the maximum effect of hydrocortisone in CAH patients is seen 3 hours after a morning dose. After an evening dose, suppression of adrenal hormones continues until approximately 0500 the next day. In both situations, however, there is a large degree of intersubject variability. These data are consistent with earlier published studies. Use of alternate specimen types, possibly in conjunction with delayed release hydrocortisone preparations under development, may allow the practitioner to design a medication regimen that provides improved control of androgen secretion. Whatever dosing strategy is used, clinical judgment is required to ensure the best outcome.</p>
Deletion of BBA64, BBA65, and BBA66 Loci Does Not Alter the Infectivity of<i>Borrelia burgdorferi</i>in the Murine Model of Lyme Disease (2008)

Borrelia burgdorferi, the causative agent of Lyme disease, alters its gene expression in response to highly disparate environmental signals encountered in its tick vector versus vertebrate hosts. Whole-genome transcriptional profile analysis of B. burgdorferi, propagated in vitro under mammalian-host-specific conditions, revealed significant upregulation of several linear plasmid 54 (lp54)-encoded open reading frames (ORFs). Among these ORFs, BBA64, BBA65, and BBA66 have been shown to be upregulated in response to multiple mammalian-host-specific signals. Recently, we determined that there was no significant difference in the ability of BBA64(-) mutant to infect C3H/HeN mice compared to its isogenic control strains, suggesting that B. burgdorferi might utilize multiple, functionally related determinants to establish infection. We further generated BBA65(-) and BBA66(-) single mutants in a noninfectious, lp25(-) clonal isolate of B. burgdorferi strain B31 (ML23) and complemented them with the minimal region of lp25 (BBE22) required for restoring the infectivity. In addition, we generated a BBA64(-) BBA65(-) BBA66(-) triple mutant using an infectious, clonal isolate of B. burgdorferi strain B31 (5A11) that has all of the infection-associated plasmids. There were no significant differences in the ability to isolate viable spirochetes from different tissues of C3H/HeN mice infected via intradermal needle inoculation with either the individual single mutants or the triple mutant compared to their respective isogenic parental strains at days 21 and 62 postinfection. These observations suggest that B. burgdorferi can establish infection in the absence of expression of BBA64, BBA65, and BBA66 in the murine model of Lyme disease.
Serological Characterization of HTLV-III Infection in AIDS and Related Disorders (1986)

Current efforts to test blood donors and other persons for exposure to the human T cell lymphotropic virus type III (HTLV-III), the etiologic agent of the acquired immunodeficiency syndrome (AIDS), are based on the measurement of serum antibodies to viral antigens. We studied presence of serum antibodies to HTLV-III-related antigens from 767 individuals with AIDS or AIDS-related complex (ARC) or asymptomatic persons at risk for AIDS by using ELISA and immunoblot techniques. Of the 280 specimens from AIDS and ARC subjects that were tested, 99% were ELISA reactive and 96% were immunoblot reactive. Greater than 96% of the seropositive subjects manifested antibodies to the p24 core antigen, whereas only 88% had antibodies to the gp41 envelope-related glycoprotein. Contrary to previous reports, a short incubation time in the immunoblot assay failed to detect low-titer or low-affinity antibodies that were detected by overnight incubation. There was no apparent difference in pattern of antibodies to HTLV-III-related antigens in symptomatic vs. asymptomatic seropositive individuals.
The mRNAs for cyclin A, c-<i>myc</i> and ribosomal proteins L4 and S6 are associated with cytoskeletal-bound polysomes in HepG2 cells (1995)

Cytosolic, cytoskeleton and membrane fractions were extracted from HepG2 cells by a sequential detergent/salt extraction procedure. The cytosolic fraction contained 93% of the lactic dehydrogenase activity while the cytoskeleton fraction was enriched in actin and vimentin. The distribution of mRNAs for c-myc, glucose transporter 1, ribosomal proteins L4 and S6 and cyclin A were investigated by Northern hybridization of total RNA extracted from polysomes isolated from cytosolic, cytoskeleton and membrane fractions. The membrane-bound polysomes were enriched in the glucose transporter 1 mRNA and the cytoskeleton-bound polysomes were enriched in the mRNAs for the two ribosomal proteins, c-myc and cyclin A. The results suggest that the mRNAs for nuclear proteins are one class of mRNAs which are translated on polysomes associated with the cytoskeleton; this may be related to the requirement to transport the newly synthesized protein to the nucleus.
Study of local intracellular signals regulating axonal morphogenesis using a microfluidic device (2016)

The establishment and maintenance of axonal patterning is crucial for neuronal function. To identify the molecular systems that operate locally to control axonal structure, it is important to manipulate molecular functions in restricted subcellular areas for a long period of time. Microfluidic devices can be powerful tools for such purposes. In this study, we demonstrate the application of a microfluidic device to clarify the function of local Ca<sup>2+</sup> signals in axons. Membrane depolarization significantly induced axonal branch-extension in cultured cerebellar granule neurons (CGNs). Local application of nifedipine using a polydimethylsiloxane (PDMS)-based microfluidic device demonstrated that Ca<sup>2+</sup> entry from the axonal region via L-type voltage-dependent calcium channels (L-VDCC) is required for branch extension. Furthermore, we developed a method for locally controlling protein levels by combining genetic techniques and use of a microfluidic culture system. A vector for enhanced green fluorescent protein (EGFP) fused to a destabilizing domain derived from <i>E. coli</i> dihydrofolate reductase (ecDHFR) is introduced in neurons by electroporation. By local application of the DHFR ligand, trimethoprim (TMP) using a microfluidic device, we were able to manipulate differentially the level of fusion protein between axons and somatodendrites. The present study revealed the effectiveness of microfluidic devices to address fundamental biological issues at subcellular levels, and the possibility of their development in combination with molecular techniques.
Evaluation of Dual Immunohistochemistry and Chromogenic In Situ Hybridization for HER2 on a Single Section (2011)

The evaluation of HER2 status in invasive breast carcinoma can be performed by multiple methods. We assessed the feasibility of performing 2 of these, chromogenic in situ hybridization (CISH) and immunohistochemical staining, on single tissue sections of breast carcinoma. During assay development, sequential performance of immunohistochemical staining after CISH resulted in weaker HER2 expression than that obtained when immunohistochemical staining was performed alone; this was ameliorated by increased antibody incubation time. Performance of both techniques in a combined/hybrid protocol resulted in HER2 protein expression and gene signals identical to those produced by the individual techniques performed alone. Prospective validation of these dual staining protocols in 31 cases of breast carcinoma resulted in 100% concordance with results of CISH when performed alone, but was still associated with a reduced immunohistochemical signal in some cases. Although further testing is needed, we conclude that performance of both immunohistochemical staining and CISH on a single section is possible and could allow for direct "cell-by-cell" comparison of HER2 signals and potentially offer a more economical and real-time method for ongoing validation of HER2 testing.
Hand-eye calibration in unstructured environments using optical theodolites (1993)

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Guide to the sun (1992)

The sun has been an object of fascination and scientific interest to humans since the time of the ancient Greeks. With minimum technical language, ...
The Tishchenko Reaction: A Classic and Practical Tool for Ester Synthesis (2006)

Abstract This article reviews the Tishchenko reaction: 2 RCHO → RCOOCH2R (R = hydrogen, alkyl, and aryl), describing the reaction mechanisms, homogeneous and heterogeneous catalysts developed for the reaction, a number of specific examples, and the utility of the reaction in the industrially large-scale production of ethyl acetate from acetaldehyde.
Preparation of Thiomer Microparticles and In Vitro Evaluation of Parameters Influencing Their Mucoadhesive Properties (2006)

It was the aim of this study to develop mucoadhesive microparticulate delivery systems based on thiomers and to investigate parameters influencing their mucoadhesive properties. Microparticles were prepared via coazervation of thiolated or unmodified polycarbophil with fluorescein-diacetate as marker. The protective effect of the polymers toward enzymatic hydrolysis by intestinal enzymes was investigated. Mucoadhesion studies with microparticles, applied in dry and prehydrated form, were performed by ascertaining their residence time on intestinal mucosa. Furthermore, the influence of the amount of thiol groups on mucoadhesion was studied in vitro. Results showed that in comparison to unmodified polycarbophil, thiolated polycarbophil provided a more than 3-fold higher protective effect for the incorporated marker fluorescein-diacetate toward hydrolysis. When being applied in dry form 23.4 +/- 4.8% of the fluorescence marker being embedded in thiomer microparticles remained adhering to the intestinal mucosa within 3 h. In contrast, only 11.6 +/- 2.0% of the marker remained on the mucosa, when the thiomer microparticles were applied in prehydrated form. In addition, tests performed to assess the impact of the amount of thiol groups pointed out that a high amount of thiol groups is advantageous in order to further improve mucoadhesive properties. This knowledge should contribute to the design of highly efficient drug delivery systems being based on thiomer microparticles.
Chloroplast Accumulation Response Enhances Leaf Photosynthesis and Plant Biomass Production (2018)

Under high light intensity, chloroplasts avoid absorbing excess light by moving to anticlinal cell walls (avoidance response), but under low light intensity, chloroplasts accumulate along periclinal cell walls (accumulation response). In most plant species, these responses are induced by blue light and are mediated by the blue light photoreceptor, phototropin, which also regulates phototropism, leaf flattening, and stomatal opening. These phototropin-mediated responses could enhance photosynthesis and biomass production. Here, using various Arabidopsis (<i>Arabidopsis thaliana</i>) mutants deficient in chloroplast movement, we demonstrated that the accumulation response enhances leaf photosynthesis and plant biomass production. Conspicuously, <i>phototropin2</i> mutant plants specifically defective in the avoidance response but not in other phototropin-mediated responses displayed a constitutive accumulation response irrespective of light intensities, enhanced leaf photosynthesis, and increased plant biomass production. Therefore, our findings provide clear experimental evidence of the importance of the chloroplast accumulation response in leaf photosynthesis and biomass production.
Insights into the genome structure and copy-number variation of Eimeria tenella (2012)

This paper describes the application of a whole genome mapping method to improve the assembly of the genome of E. tenella from shotgun data, and to help reveal its overall structure. A preliminary assessment of copy-number variation (extra or missing copies of genomic segments) between strains of E. tenella was also carried out. The emerging picture is of a very unusual genome architecture displaying inter-strain copy-number variation. We suggest that these features may be related to the known ability of this parasite to rapidly develop drug resistance.
Vancomycin Resistance: Structure of D-Alanine:D-Alanine Ligase at 2.3 Å Resolution (1994)

The molecular structure of the D-alanine:D-alanine ligase of the ddlB gene of Escherichia coli, co-crystallized with an S,R-methylphosphinate and adenosine triphosphate, was determined by x-ray diffraction to a resolution of 2.3 angstroms. A catalytic mechanism for the ligation of two D-alanine substrates is proposed in which a helix dipole and a hydrogen-bonded triad of tyrosine, serine, and glutamic acid assist binding and deprotonation steps. From sequence comparison, it is proposed that a different triad exists in a recently discovered D-alanine:D-lactate ligase (VanA) present in vancomycin-resistant enterococci. A molecular mechanism for the altered specificity of VanA is suggested.
100GbE PHY and MAC layer implementations (2010)

This article discusses the logical implementation of the media access control and the physical layer of 100 Gb/s Ethernet. The target are a MAC/PCS LSI, supporting MAC and physical coding sublayer, and a gearbox LSI, providing 10:4 parallel lane-width exchange inside an optical module. The two LSIs are connected by a 100 gigabit attachment unit interface, which consists of ten 10 Gb/s lines. We realized a MAC/PCS logical circuit with a low-frequency clock on a FPGA, whose size is 250 kilo LUTs with a 5.7 Mbit RAM, and the power consumption of the gearbox LSI estimated to become 2.3 W.
Glucose transport in lysosomal membrane vesicles. Kinetic demonstration of a carrier for neutral hexoses. (1990)

Lysosomal membrane vesicles isolated from rat liver were exploited to analyze the mechanism of glucose transport across the lysosomal membrane. Uptake kinetics of [14C]D-glucose showed a concentration-dependent saturable process, typical of carrier-mediated facilitated transport, with a Kt of about 75 mM. Uptake was unaffected by Na+ and K+ ions, membrane potentials, and proton gradients but showed an acidic pH optimum. Lowering the pH from 7.4 to 5.5 had no effect on the affinity of the carrier for the substrate but increased the maximum rate of transport about 3-fold. As inferred from the linearity of Scatchard plots, a single transport mechanism could account for the uptake of glucose under all conditions tested. As indicated by the transstimulation properties of the carrier, other neutral monohexoses, including D-galactose, D-mannose, D- and L-fucose were transported by this carrier. The transport rates and affinities of these sugars, measured by the use of their radiolabeled counterparts, were in the same range as those for D-glucose. Pentoses, sialic acid, and other acidic monosaccharides including their lactones, aminosugars, N-acetyl-hexosamines, and most L-stereoisomers, particularly those not present in mammalian tissues, were not transported by this carrier. Glucose uptake and transstimulation were inhibited by cytochalasin B and phloretin. The biochemical properties of this transporter differentiate it from other well-characterized lysosomal sugar carriers, including those for sialic acid and N-acetylhexosamines. The acidic pH optimum of this glucose transporter is a unique feature not shared with any other known glucose carrier and is consistent with its lysosomal origin.
Band-Preference Dynamic Spectrum Management in a DSL Environment (2006)

Abstract — This paper introduces an algorithm for spectrum management for digital subscriber line (DSL) systems based on band preference. The proposed method influences the usage of spectrum through band preference factors that subtly modify the loading algorithm of DSL modems. Ad-hoc algorithms for computing such band preference factors are discussed. Simulation results in a practical ADSL environment show that the performance of the proposed method is better than that of Iterative Water-filling (IWF) [1] and is close to that of Optimal Spectrum Balancing (OSB) [2], even with a small number of control parameters. I.
Effects of Carbon Dioxide Injection in Reactive Carbonates: Computational Rock Physics Basis for Time-Lapse Monitoring (2011)

Abstract Carbon dioxide injection into natural reservoirs has been used for enhanced oil recovery as well as, more recently, for geologic sequestration. In both technological applications, of major interest is remote sensing of the progress of the injected carbon dioxide through the subsurface as well as its effects on the physical characteristics of the rock, including the elastic, storage, and transport properties. Whereas it may be safe to assume that the presence of carbon dioxide does not alter the mineral matrix in most clastic reservoirs, the situation is very different in carbonates where such alteration may occur in real time, in a matter of hours, days and, certainly, months. Physical laboratory experiments have confirmed that the carbon dioxide interacts with formation water and, eventually, with the mineral matrix (Vanorio, T. et all, 2008). These chemical processes alter the pore space geometry in carbonates and can even create such flow conduits as relatively large wormholes. This pore-space and matrix alterations can definitely affect the elastic properties of carbonate rock, which are crucial in interpreting the 4D time-lapse seismic data for pore-fluid content in space and time. Because of the changes occurring in the mineral matrix during carbon dioxide injection, traditional fluid-substitution techniques often do not work in such reactive rock and, hence, can be misleading during seismic data interpretation.
Deep Reactive Ion Etching (DRIE) of High Aspect Ratio SiC Microstructures Using a Time-Multiplexed Etch-Passivate Process (2006)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Adaptive structured parallelism (2008)

Algorithmic skeletons abstract commonly-used patterns of parallel computation, communication, and interaction. Parallel programs are expressed by interweaving parameterised skeletons analogously to the way in which structured sequential programs are developed, using well-defined constructs. Skeletons provide top-down design composition and control inheritance throughout the program structure. Based on the algorithmic skeleton concept, structured parallelism provides a high-level parallel programming technique which\nallows the conceptual description of parallel programs whilst fostering platform independence and algorithm abstraction. By decoupling the algorithm\nspecification from machine-dependent structural considerations, structured parallelism allows programmers to code programs regardless of how the computation and communications will be executed in the system platform.
Bimatoprost, a novel efficacious ocular hypotensive drug now recognized as a member of a new class of agents called prostamides (2007)

Abstract Pursuit of a new FP‐agonist prodrug led to the identification of an interesting series of neutral C1‐substituted prostaglandin F 2α analogues. Although these initial analogues were devoid of any inherent pharmacological activity at the FP‐receptor, two compounds AGN‐190910 and AGN‐191129, were found to have pronounced effects in lowering intraocular pressure (IOP) in normotensive dogs and monkeys. The cat iris sphincter assay was quickly developed as a primary screen for these analogues, leading to rapid identification of AGN‐192024 (17‐phenyl PGF 2α ethyl amide, bimatoprost). While bimatoprost is structurally similar to naturally occurring mammalian hormones of the prostanoid family, surprisingly it demonstrates no significant activity at any of the known prostanoid receptors. Furthermore, results of considerable additional pharmacological studies provide evidence that it may indeed act through a unique receptor yet to be identified. The effect of Bimatoprost on lowering IOP has also been found to be unique in comparison to prostanoids. Bimatoprost reduces human IOP by increasing aqueous humor outflow through a dual mechanism of action where it improves both pressure‐dependent and pressure‐independent outflow pathways. First introduced to the market in 2002, bimatoprost is currently the most potent single therapy available for control of ocular hypertension. Drug Dev Res 68:147–155, 2007. ©2007 Wiley‐Liss, Inc.
Hepatocyte Growth Factor in Saliva of Patients with Renal Failure and Periodontal Disease (2012)

The HGF level in mixed saliva is the index of PD progression in subjects without renal failure and in CAPD-treated patients. PD is common in renal failure patients and is a significant problem concerning general health status.