gebhart/patrepeval huggingface dataset:
Fluorometric determination of total vitamin C and total isovitamin C in foodstuffs and beverages by high-performance liquid chromatography with precolumn derivatization (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFluorometric determination of total vitamin C and total isovitamin C in foodstuffs and beverages by high-performance liquid chromatography with precolumn derivatizationAndries J. Speek, Jaap Schrijver, and Wil H. P. SchreursCite this: J. Agric. Food Chem. 1984, 32, 2, 352–355Publication Date (Print):March 1, 1984Publication History Published online1 May 2002Published inissue 1 March 1984https://pubs.acs.org/doi/10.1021/jf00122a043https://doi.org/10.1021/jf00122a043research-articleACS PublicationsRequest reuse permissionsArticle Views465Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
New live screening of plant-nematode interactions in the rhizosphere (2018)

Free living nematodes (FLN) are microscopic worms found in all soils. While many FLN species are beneficial to crops, some species cause significant damage by feeding on roots and vectoring viruses. With the planned legislative removal of traditionally used chemical treatments, identification of new ways to manage FLN populations has become a high priority. For this, more powerful screening systems are required to rapidly assess threats to crops and identify treatments efficiently. Here, we have developed new live assays for testing nematode responses to treatment by combining transparent soil microcosms, a new light sheet imaging technique termed Biospeckle Selective Plane Illumination Microscopy (BSPIM) for fast nematode detection, and Confocal Laser Scanning Microscopy for high resolution imaging. We show that BSPIM increased signal to noise ratios by up to 60 fold and allowed the automatic detection of FLN in transparent soil samples of 1.5 mL. Growing plant root systems were rapidly scanned for nematode abundance and activity, and FLN feeding behaviour and responses to chemical compounds observed in soil-like conditions. This approach could be used for direct monitoring of FLN activity either to develop new compounds that target economically damaging herbivorous nematodes or ensuring that beneficial species are not negatively impacted.
Cutaneous Innervation of Lower Eyelid (2008)

The loss of skin sensation or numbness after lower blepharoplasty is not uncommon. The aim of this study is to elucidate the infraorbital nerve (ION) and zygomaticofacial nerve (ZFN) in detail. Twenty-one hemifaces of 14 fresh Korean adult cadavers were dissected. Infraorbital nerve and ZFN came out of infraorbital foramen and zygomaticofacial foramen. They ran along superficial to the periosteum within and beneath the epimysium of the orbicularis oculi muscle and then through orbicularis oculi muscle perpendicularly and distributed to the skin. The distal branch approached to the lower border of the tarsal plate. Most terminal branches (93.8%) of ION were distributed medial to the lateral canthus. Only a few branches (6.2%) were lateral to the lateral canthus. Most (99.4%) terminal branches of ZFN were distributed lateral to the lateral canthus. Very few (0.6%) branches were medial to the lateral canthus. We conclude that the skin-muscle flap infringes less than the skin flap on the terminal branches of ION and ZFN in exposure of the orbital floor as well as in lower blepharoplasty.
Situational Awareness Using the Responsive Workbench (1997)

Abstract : Our task is to provide situational awareness for the complex logistical task of directing the movement of U.S. Marines and material over rugged terrain, day and night, in uncertain weather conditions. This difficulty is multiplied by the well-known dangers of amphibious assault, long considered the most difficult problem in warfare. Even with the advent of computers and sophisticated decision-making software in Marine Corps Combat Operation Centers, command and control is predominantly undertaken with paper maps and acetate overlays. This is a cumbersome, time consuming process. In addition, detailed maps and overlays can take several hours to print and distribute. There currently exists no overall picture of the battlespace that provides a commander with a dynamic range of resolution sufficient to track units ranging from aircraft carriers to six-Marine fire teams.
Unequally Powered Cryptography With Physical Unclonable Functions for Networks of Internet of Things Terminals (2019)

In this paper, cryptographic architectures based on unequal computing resources are modelled, and experimentally verified with Window-10 PCs powered with Intel I-7 processor. Low power IoT terminal devices generate keys from physical unclonable functions, which can be recognized by the server through a matching engine. We are here proposing methods to modulate the efficiency of the matching engine by injecting erratic bits in the keys, and/or by fragmenting the keys in smaller sub-keys. When the environment is hostile, the difficulty in matching the keys can be adjusted in such a way that the server needs access to increasingly powerful computing power to recognize the terminal device thereby placing attackers with inferior computing power at a strong disadvantage. The objective of this research work is to find, through the modelling effort, the conditions where unequally powered cryptography can take advantage of high performance computers to protect networks of IoT terminals.
Transcriptomic responses to sodium chloride‐induced osmotic stress: A study of industrial fed‐batch CHO cell cultures (2010)

The rapidly expanding market for monoclonal antibody and Fc-fusion-protein therapeutics has increased interest in improving the productivity of mammalian cell lines, both to alleviate capacity limitations and control the cost of goods. In this study, we evaluated the responses of an industrial CHO cell line producing an Fc-fusion-protein to hyperosmotic stress, a well-known productivity enhancer, and compared them with our previous studies of murine hybridomas (Shen and Sharfstein, Biotechnol Bioeng. 2006;93:132-145). In batch culture studies, cells showed substantially increased specific productivity in response to increased osmolarity as well as significant metabolic changes. However, the final titer showed no substantial increase due to the decrease in viable cell density. In fed batch cultures, hyperosmolarity slightly repressed the cellular growth rate, but no significant change in productivity or final titer was detected. To understand the transcriptional responses to increased osmolarity and relate changes in gene expression to increased productivity and repressed growth, proprietary CHO microarrays were used to monitor the transcription profile changes in response to osmotic stress. A set of osmotically regulated genes was generated and classified by extracting their annotations and functionalities from online databases. The gene list was compared with results previously obtained from similar studies of murine-hybridoma cells. The overall transcriptomic responses of the two cell lines were rather different, although many functional groups were commonly perturbed between them. Building on this study, we anticipate that further analysis will establish connections between productivity and the expression of specific gene(s), thus allowing rational engineering of mammalian cells for higher recombinant-protein productivity.
Heterodyne frequency measurements of a voltage-controlled diode laser (1993)

A single-mode GaAlAs diode laser is frequency stabilized by keeping the injection current and the voltage across the laser constant. The stability of the laser is studied by heterodyning it with another laser locked to the center of a hyperfine transition of the D/sub 2/-line of /sup 85/Rb. Alternatively, the frequency stability is observed by tuning the voltage-controlled diode laser to the center of an absorption line of rubidium vapor and recording changes in the absorption signal. In both cases a long-term frequency stability of 4*10/sup -8/ is observed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Bacitracin is not a specific inhibitor of protein disulfide isomerase (2010)

To successfully dissect molecular pathways in vivo, there is often a need to use specific inhibitors. Bacitracin is very widely used as an inhibitor of protein disulfide isomerase (PDI) in vivo. However, the specificity of action of an inhibitor for a protein-folding catalyst cannot be determined in vivo. Furthermore, in vitro evidence for the specificity of bacitracin for PDI is scarce, and the mechanism of inhibition is unknown. Here, we present in vitro data showing that 1 mM bacitracin has no significant effect on the ability of PDI to introduce or isomerize disulfide bonds in a folding protein or on its ability to act as a chaperone. Where bacitracin has an effect on PDI activity, the effect is relatively minor and appears to be via competition of substrate binding. Whereas 1 mM bacitracin has minimal effects on PDI, it has significant effects on both noncatalyzed protein folding and on other molecular chaperones. These results suggest that the use of bacitracin as a specific inhibitor of PDI in cellular systems requires urgent re-evaluation.
Patterned growth of nanocrystalline silicon thin films through magnesiothermic reduction of soda lime glass (2012)

A low-cost and green method of producing nanocrystalline silicon thin films is presented. Using a magnesiothermic reduction process, we have successfully converted the surface of soda lime glass directly into silicon thin film. Furthermore, by varying reaction time, the amount of silicon produced in thin film form (or layer thickness) can be controlled. The nanocrystalline silicon thin films on glass were characterized using scanning electron microscopy, energy dispersive spectroscopy and Raman spectroscopy. Finally, the optical properties of the thin films derived at different reaction times were also measured. The band gaps of the synthesized thin films were within the range of 2.3–2.5 eV.
Variable selection techniques in discriminant analysis: I. Description (1982)

The problem of assessing the relative importance of variable subsets in discriminant analysis is discussed. Attention is focused on techniques for determining those subsets which are ‘adequate’ for discrimination from the descriptive viewpoint. A number of procedures which have been proposed or used in the literature are described, illustrated and compared with reference to various aspects, including rationale, statistical significance testing and computational difficulties.
On verifying the correctness of retimed circuits (2002)

We address the problem of verifying a retimed circuit. After retiming, some latches in a sequential circuit are repositioned to reduce the clock cycle time and thus the behavior of the combinational portion is changed. Here, we present a novel approach to check the correctness of a retimed circuit according to the definition of 3-valued equivalence. This approach is based on our verification framework using sequential ATPG techniques. We further incorporate an algorithm to pre-process the circuits and make the verification process even more efficient. We will present the experimental results of verifying the retimed circuits with hundreds of flip-flops on ISCAS89 benchmark circuits to show its capability.
Comment on ‘‘Scanned-cantilever atomic force microscope’’ [Rev. Sci. Instrum. <b>64</b>, 908 (1993)] (1994)

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Cutting Edge: Toll-Like Receptor 4 (TLR4)-Deficient Mice Are Hyporesponsive to Lipopolysaccharide: Evidence for TLR4 as the <i>Lps</i> Gene Product (1999)

The human homologue of Drosophila Toll (hToll), also called Toll-like receptor 4 (TLR4), is a recently cloned receptor of the IL-1/Toll receptor family. Interestingly, the TLR4 gene has been localized to the same region to which the Lps locus (endotoxin unresponsive gene locus) is mapped. To examine the role of TLR4 in LPS responsiveness, we have generated mice lacking TLR4. Macrophages and B cells from TLR4-deficient mice did not respond to LPS. All these manifestations were quite similar to those of LPS-hyporesponsive C3H/HeJ mice. Furthermore, C3H/HeJ mice have, in the cytoplasmic portion of TLR4, a single point mutation of the amino acid that is highly conserved among the IL-1/Toll receptor family. Overexpression of wild-type TLR4 but not the mutant TLR4 from C3H/HeJ mice activated NF-kappaB. Taken together, the present study demonstrates that TLR4 is the gene product that regulates LPS response.
Reactivation of Mutant p53 through Interaction of a C-Terminal Peptide with the Core Domain (1999)

A synthetic 22-mer peptide (peptide 46) derived from the p53 C-terminal domain can restore the growth suppressor function of mutant p53 proteins in human tumor cells (G. Selivanova et al., Nat. Med. 3:632-638, 1997). Here we demonstrate that peptide 46 binds mutant p53. Peptide 46 binding sites were found within both the core and C-terminal domains of p53. Lys residues within the peptide were critical for both p53 activation and core domain binding. The sequence-specific DNA binding of isolated tumor-derived mutant p53 core domains was restored by a C-terminal polypeptide. Our results indicate that C-terminal peptide binding to the core domain activates p53 through displacement of the negative regulatory C-terminal domain. Furthermore, stabilization of the core domain structure and/or establishment of novel DNA contacts may contribute to the reactivation of mutant p53. These findings should facilitate the design of p53-reactivating drugs for cancer therapy.
Haemophilus Influenzae Type b (Hib) Antibody Concentrations and Vaccination Responses in Patients with Chronic Lymphocytic Leukaemia: Predicting Factors for Response (2002)

We have recently demonstrated a moderate vaccination response rate of 43% against Haemophilus influenzae type b (Hib) conjugate vaccine among adult and elderly patients with chronic lymphocytic leukaemia (CLL). We now investigated demographic and immunological factors predicting the favourable response and protective antibody concentrations for Hib conjugate vaccine in CLL. Lower age was associated with protective pre- and post-vaccination antibody concentrations. High IgG1 and IgA concentrations were also associated with the protective efficacy. High IgM, in turn, was the best predictor of a significant vaccination response. Again, lower age seemed to be involved in this outcome. Judging from these findings, it would seem beneficial to vaccinate all CLL patients with conjugate vaccines at the presentation of the disease. Investigations of a new pneumococcal conjugate vaccine in CLL are warranted.
Statistical Parametric Speech Synthesis Using Bottleneck Representation From Sequence Auto-encoder (2016)

In this paper, we describe a statistical parametric speech synthesis approach with unit-level acoustic representation. In conventional deep neural network based speech synthesis, the input text features are repeated for the entire duration of phoneme for mapping text and speech parameters. This mapping is learnt at the frame-level which is the de-facto acoustic representation. However much of this computational requirement can be drastically reduced if every unit can be represented with a fixed-dimensional representation. Using recurrent neural network based auto-encoder, we show that it is indeed possible to map units of varying duration to a single vector. We then use this acoustic representation at unit-level to synthesize speech using deep neural network based statistical parametric speech synthesis technique. Results show that the proposed approach is able to synthesize at the same quality as the conventional frame based approach at a highly reduced computational cost.
GAA Trinucleotide Repeat Region Regulates M9/pMGA Gene Expression in <i>Mycoplasma gallisepticum</i> (2000)

Mycoplasma gallisepticum, the cause of chronic respiratory infections in the avian host, possesses a family of M9/pMGA genes encoding an adhesin(s) associated with hemagglutination. Nucleotide sequences of M9/pMGA gene family members indicate extensive sequence similarity in the promoter regions of both the transcribed and silent genes. The mechanism that regulates M9/pMGA gene expression is unknown, but studies have revealed an apparent correlation between gene expression and the number of tandem GAA repeat motifs located upstream of the putative promoter. In this study, transposon Tn4001 was used as a vector with the Escherichia coli lacZ gene as the reporter system to examine the role of the GAA repeats in M9/pMGA gene expression in M. gallisepticum. A 336-bp M9 gene fragment (containing the GAA repeat region, the promoter, and the translation start codon) was amplified by PCR, ligated with a lacZ gene from E. coli, and inserted into the Tn4001-containing plasmid pISM2062. This construct was transformed into M. gallisepticum PG31. Transformants were filter cloned on agar supplemented with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) to monitor lacZ gene expression on the basis of blue/white color selection. Several cycles of filter cloning resulted in cell lineages in which lacZ gene expression alternated between the On and Off states in successive generations of progeny clones. The promoter regions of the M9-lacZ hybrid genes of individual progeny clones were amplified by PCR and sequenced. The only differences between the promoter regions of the blue and white colonies were in the number of GAA repeats. Clones that expressed lacZ had exactly 12 tandem copies of the GAA repeat. Clones that did not express lacZ invariably had either more than 12 (14 to 16) or fewer than 12 (5 to 11) GAA repeats. Southern analysis of M. gallisepticum chromosomal DNA confirmed that the phase-variable expression of the lacZ reporter gene was not caused by Tn4001 transposition. These data strongly indicate that changes in the length of the GAA repeat region are responsible for regulating M9/pMGA gene expression.
Design and Evaluation of an LED-based Light Fixture (2016)

Interior spaces, such as conference rooms, require multiple forms of lighting to meet different task needs. Linear fluorescent lamp fixtures commonly produce the general ambient light, and reflectorized halogen lamp fixtures produce the directional lighting. Current lighting practice uses multiple light source technologies and fixtures to achieve the required illumination for the various tasks. However, many light fixtures can create an unappealing architectural design, especially in a small space, and multiple light source technologies can cause maintenance difficulties. The ideal scenario would be to create one fixture with a single type of light source that could meet a variety of lighting needs. The size, potential energy savings, and reduced maintenance benefits of white light-emitting diodes (LEDs) make them attractive for use in general illumination applications. Therefore, the goal of this study was to investigate whether a light fixture could be designed with white LEDs to provide different beam distributions. A commercially available ray tracing package was utilized to model, analyze, and optimize light fixture concepts created using LEDs and light guides. The fixtures were optimized for light output distribution and efficiency. While a rectangular-shaped light guide with simple diffused reflective surfaces provided the necessary cosine beam distribution, a more sophisticated surface treatment was required on the light extracting surface of the light guide to create the batwing beam distributions. The surface finish structures were in the form of micro-prism arrays. By switching LED arrays on and off to different light guides, various
Analysis of the antimetastatic effects of synthetic muramyl tripeptide (CGP 19835A) encapsulated in liposomes in combination with other immunomodulatory agents and chemotherapeutic drugs. (1994)

The synthetic molecule muramyl tripeptide (CGP 19835A) encapsulated in liposomes is effective in increasing the survival of mice with spontaneous experimental lung metastases induced by the RENCA renal adenocarcinoma and B16 melanoma tumor models. The present study was aimed at extending the effects of CGP 19835A to another highly metastatic carcinoma model and at evaluating the efficacy of combination therapy with standard cytotoxic agents and other immunomodulators. C57BL/6 mice received whole tumor implants of PancO2, a spontaneously metastasizing pancreatic adenocarcinoma, subcutaneously in the hind leg. Therapeutic effects were measured by increased survival which is a direct function of the growth of spontaneous lung metastases in this system. No therapeutic efficacy was observed with CGP 19835A alone or in combination with any of a series of cytotoxic or biological agents, including cis-platinurn (cis-Pt), mitomycin C (MMC), adriamycin (ADR), cyclophosphamide (CP), interferon gamma (IFN gamma), and interleukin 2 (IL-2). In accord with previous studies, when the B16-F10 melanoma was used as an experimental metastatic tumor model, CGP 19835A, alone and in combination with CP, significantly reduced the number of pulmonary metastases. Cis-Pt, however, partially negated the effects of CGP 19835A when a combination of the two agents was used. The results indicate that CGP 19835A is an effective therapeutic agent in some models of spontaneous or experimental lung metastases, but not others, and that the effects of CGP 19835A are not enhanced by the accompanying cytotoxic drugs tested here.
Mutual Coupling Reduction for UWB MIMO Antennas With a Wideband Neutralization Line (2015)

A wideband neutralization line is proposed to reduce the mutual coupling of a compact ultrawideband (UWB) multiple-input-multiple-output (MIMO) antenna. With the introduced decoupling method, the designed UWB MIMO antenna covers the band of 3.1-5 GHz with an isolation of higher than 22 dB. The proposed wideband neutralization line is not necessarily placed in the clearance area between two MIMO elements and can be put above the copper ground. A small clearance (antenna area) of 35 ×16 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> is achieved. The designed UWB MIMO antenna is fabricated. Sparameters, radiation patterns, total efficiency, and realized gain of the prototype are measured and compared to the simulations.