gebhart/patrepeval huggingface dataset:
A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA (2013)

Several methods for the detection of RNA have been developed over time. For small RNA detection, a stem-loop reverse primer-based protocol relying on TaqMan RT-PCR has been described. This protocol requires an individual specific TaqMan probe for each target RNA and, hence, is highly cost-intensive for experiments with small sample sizes or large numbers of different samples. We describe a universal TaqMan-based probe protocol which can be used to detect any target sequence and demonstrate its applicability for the detection of endogenous as well as artificial eukaryotic and bacterial small RNAs. While the specific and the universal probe-based protocol showed the same sensitivity, the absolute sensitivity of detection was found to be more than 100-fold lower for both than previously reported. In subsequent experiments, we found previously unknown limitations intrinsic to the method affecting its feasibility in determination of mature template RISC incorporation as well as in multiplexing. Both protocols were equally specific in discriminating between correct and incorrect small RNA targets or between mature miRNA and its unprocessed RNA precursor, indicating the stem-loop RT-primer, but not the TaqMan probe, triggers target specificity. The presented universal TaqMan-based RT-PCR protocol represents a cost-efficient method for the detection of small RNAs.
Viscoelastic Properties of Pressure-Sensitive Adhesives (1997)

Abstract Recent studies on the correlation of viscoelastic properties of pressure-sensitive adhesive (PSAs) with industry standard performances such as peel, tack and shear are reviewed and discussed. One notewothy feature in these correlations is the separation of the bonding and debonding steps in PSA adhesion, which specifies the characteristic bonding and debonding frequencies of different PSA tests. Viscoelastic windows (VW) for different types of pressure-sensitive adhesives (PSAs) proposed by these workers are also compared and discussed. The observed good correlations reaffirm the importance of bulk viscoelastic properties to PSA adhesion performances. Key Words: Pressure-sensitive adhesivesdynamic mechanical analysisviscoelastic propertiesdynamic storage and loss modulibonding and debonding frequenciespeelshear and tack performanceviscoelastic windows
Wirtschaftspolitische Interventionen und Landwirtschaft (1956)

Agricultural and Food Policy
Review of Inventory of Main Holdings of the Sound Archives (1979)

The Public Archives of Canada has just published INVENTORY OF MAIN HOLDINGS OF THE SOUND ARCHIVES / REPERTOIRE SELECTIF DES ARCHIVES SONORES. This is the first of a projected series of publications designed to inform researchers, educators, and broadcasters of the historical sound recordings held at the Public Archives.
Powered Flight of Electron Cyclotron Resonance Ion Engines on Hayabusa Explorer (2007)

The electron cyclotron resonance ion engine has long life and high reliability because of electrodeless plasma generation in both the ion generator and the neutralizes Four μ 10s, each generating a thrust of 8 mN, specific impulse of 3200 s, and consuming 350 W of electric power, propelled the Hayabusa asteroid explorer launched on May 2003. After vacuum exposure and several baking runs to reduce residual gas, the ion engine system established continuous acceleration. Electric propelled delta-V Earth gravity assist, a new orbit change scheme that uses electric propulsion with a high specific impulse was applied to change from a terrestrial orbit to an asteroid-based orbit In 2005, Hayabusa, using solar electric propulsion, managed to successfully cover the solar distance between 0.86 and 1.7 AU. It rendezvoused with, landed on, and lifted off from the asteroid Itokawa. During the 2-year flight, the ion engine system generated a delta-V of 1400 m/s while consuming 22 kg of xenon propellent and operating for 25,800 h.
Proposed Experiment to Test Local Hidden-Variable Theories (1969)

A theorem of Bell, proving that certain predictions of quantum mechanics are inconsistent with the entire family of local hidden-variable theories, is generalized so as to apply to realizable experiments. A proposed extension of the experiment of Kocher and Commins, on the polarization correlation of a pair of optical photons, will provide a decisive test between quantum mechanics and local hidden-variable theories.
Rheumatologic manifestations of gastrointestinal disorders (1999)

There are many different rheumatic symptoms of gastrointestinal (GI) disorders and a whole range of GI symptoms that occur in rheumatologic disorders. Spondyloarthropathies (SpA) are closely related to the GI tract. Bacterial DNA has been detected in peripheral but not the sacroiliac joints of patients, including enterobacteria; the significance of this finding is not clear yet because antibiotic therapy seems not to be effective. The synovial lymphocyte proliferation of reactive arthritis (ReA) patients to the 60-kD heat-shock protein of Yersinia plays a major role in the immune response. Anti-Klebsiella antibodies are associated with gut lesions in SpA; their significance for the pathogenesis of SpA remains uncertain. ReA patients seem to have an impaired TH1-cytokine response, which might contribute to disease persistence. HLA B27-positive subjects seem to have a low tumor necrosis factor-alpha secretor status, possibly leading to diminished immune responses against certain microbes. In patients with Whipple's disease, Tropheryma whippelii can be cultured from gut biopsy specimens when interleukin-4 is added. The gene for hemochromatosis has been identified. Hepatitis C virus DNA can be found in many patients with cryoglobulinemia. Treatment with interferon-alpha might help in some patients. Effective treatment for primary biliary cirrhosis with ursodeoxycholic acid is not helpful for rheumatic symptoms. The severity of esophageal dysfunction in systemic sclerosis does not correlate with symptoms. GI symptoms do, although not frequently, occur in vasculitides.
Defects of graphene on Ir(111): Rotational domains and ridges (2009)

We use low-energy electron microscopy (LEEM), low-energy electron diffraction (LEED), and scanning tunneling microscopy (STM) to study different orientations of single-layer graphene sheets on Ir(111). The most-abundant orientation has previously been characterized in the literature. Using selective-area LEED we find three other variants, which are rotated $14\ifmmode^\circ\else\textdegree\fi{}$, $18.5\ifmmode^\circ\else\textdegree\fi{}$, and $30\ifmmode^\circ\else\textdegree\fi{}$ with respect to the most common variant. The $\ensuremath{\sim}30\ifmmode^\circ\else\textdegree\fi{}$-rotated structure is also studied by STM. We propose that all four variants are moir\'e structures that can be classified using simple geometric rules involving periodic and quasiperiodic structural motifs. In addition, LEEM reveals that linear defects form in the graphene sheets during cooling from the synthesis temperature. STM shows that these defects are ridges, suggesting that the graphene sheets delaminate locally as the Ir substrate contracts.
Non-invasive monitoring and control in silicon photonics using CMOS integrated electronics (2014)

As photonics moves from the single-device level toward large-scale, integrated, and complex systems on a chip, monitoring, control, and stabilization of the components become critical. We need to monitor a circuit non-invasively and apply a simple, fast, and robust feedback control. Here, we show non-invasive monitoring and feedback control of high-quality-factor silicon (Si) photonic resonators assisted by a transparent detector that is directly integrated inside the cavity. Control operations are entirely managed by a CMOS microelectronic circuit that is bridged to the Si photonic chip and hosts many parallel electronic readout channels. Advanced functionalities, such as wavelength tuning, locking, labeling, and swapping, are demonstrated. The non-invasive nature of the transparent monitor and the scalability of the CMOS readout system offer a viable solution for the control of arbitrarily reconfigurable photonic integrated circuits aggregating many components on a single chip.
Efficient Index Structures for String Databases (2001)

We consider the problem of substring searching in large databases. Typical applications of this problem are genetic data, web data, and event sequences. Since the size of such databases grows exponentially, it becomes impractical to use inmemory algorithms for these problems. In this paper, we propose to map the substrings of the data into an integer space with the help of wavelet coefficients. Later, we index these coefficients using MBRs (Minimum Bounding Rectangles). We define a distance function which is a lower bound to the actual edit distance between strings. We experiment with both nearest neighbor queries and range queries. The results show that our technique prunes significant amount of the database (typically 50-95%), thus reducing both the disk I/O cost and the CPU cost significantly .
Deblurring Methods Using Antireflective Boundary Conditions (2008)

In this paper we consider the numerical solution of self-adjoint deblurring problems on bounded intervals. For these problems it has recently been shown that appropriate modeling of the solution near the boundary of the interval may significantly improve the numerical reconstructions. Among the alternatives the so-called antireflective boundary condition appears to be the best known choice. Here we develop an appropriate, i.e., stable and efficient implementation of this model in two steps, namely by (i) transforming the problem to homogeneous boundary values, and (ii) using the fast sine transform to solve the transformed problem. This approach allows us to incorporate regularization in a very straightforward way. Numerical reconstructions are superior qualitatively and quantitatively to those obtained with the reblurring method of Donatelli and Serra-Capizzano.
Copper-64-diacetyl-bis( <i>N</i> <sup>4</sup> -methylthiosemicarbazone): An agent for radiotherapy (2001)

Systemic administration of hypoxia-selective (64)Cu-diacetyl-bis(N(4)-methylthiosemicarbazone) ((64)Cu-ATSM) has increased significantly the survival time of hamsters bearing human GW39 colon cancer tumors. Radiotherapy experiments were performed in animals bearing either 7-day-old (0.5-1.0 g) or 15-day-old (1.5-2.0 g) tumors. Studies compared animals treated with a single dose of 0, 4, 6, 7, 8, or 10 mCi of (64)Cu-ATSM (1 Ci = 37 GBq) with or without the vasodilator hydralazine. A multiple dose regimen of 3 x 4 mCi at 72-h intervals was studied also. Single doses of >6 mCi of (64)Cu-ATSM and the dose-fractionation protocol significantly increased the survival time of the hamsters compared with controls. The highest dose, 10 mCi of (64)Cu-ATSM, increased survival to 135 days in 50% of animals bearing 7-day-old tumors, 6-fold longer than control animals' survival (20 days), with only transient leucopenia and thrombocytopenia but no overt toxicity. Human absorbed doses were calculated from hamster biodistribution; the dose-critical organs were the lower large intestine (1.43 +/- 0.19 rad/mCi) and upper large intestine (1.20 +/- 0.38 rad/mCi). High-resolution MRI and positron-emission tomography using a therapeutic administration of 10 mCi were used to monitor tumor volume and morphology and to assess tumor dosimetry accurately, giving a tumor dose of 81 +/- 7.5 rad/mCi. (64)Cu-ATSM has increased the survival time of tumor-bearing animals significantly with no acute toxicity and thus is a promising agent for radiotherapy.
Multipass amplification of soft x rays in a laser cavity (1988)

We report the first demonstration to our knowledge of multipass amplification of soft x rays. A gain medium of neonlike selenium ions was placed within a resonant cavity composed of a multilayer mirror and a beam splitter designed for normal-incidence use at the 20.63- and 20.96-nm laser lines of the neonlike selenium. The laser-cavity output was time resolved and exhibited three distinct temporal components identifiable as the single-, double-, and triple-pass amplified emission. In these experiments, multipass amplification was limited by the finite duration of the gain medium.
Noncognitive Variables in Predicting Academic Success by Race (1984)

The purpose of this study was to examine the reliability, construct validity, and predictive validity of the Non-Cognitive Questionnaire for different racial groups. The questionnaire had adequate test-retest reliability, and evidence for its construct validity was provided. It significantly added to predicting grades for both White and Black subsamples and significantly predicted persistence for the Black subsamples.
Use of a TiN barrier to improve GaAs FET ohmic contact reliability (1985)

We have used a reactively sputtered TiN diffusion barrier to prevent interpenetration of a (Ni)GeAuPt ohmic contact layer and Ti/Pt/Au overlay on GaAs devices baked at 250-300°C in air. Planar GaAs MESFET's and TLM patterns were fabricated and iteratively tested and baked. Devices without TiN showed severe degradation in morphology and dc and RF performance. Devices with TiN remained essentially unchanged.
Microstructure-property relationships in high chromium white iron alloys (1996)

AbstractAbstractHigh chromium white irons are ferrous based alloys containing 11–30 wt-% chromium and 1.8–3.6 wt-% carbon, with molybdenum, manganese, copper, and nickel sometimes added as additional alloying elements. The microstructure of these alloys typically consists of hard primary and/or eutectic carbides in a matrix of austenite or one of its transformation products. The presence of hard alloy carbides results in excellent abrasion resistance and, consequently, these alloys are commonly used for materials handling in the mining and minerals processing industries. Alloy content, solidification parameters, and thermal processing can dramatically alter the microstructure that is produced, and this in turn can influence the properties and hence performance of white iron alloys during service. This review outlines the development of the microstructure in high chromium white irons through solidification and thermal processing. The metallurgical effects of conventional processing techniques are discussed, and advances in aspects such as alloying and cryogenic treatments covered. The results of laboratory abrasion tests are summarised, and the effect of microstructure on the wear properties are discussed. The toughness and impact resistance of white cast irons, which are often thought to represent a limiting factor in their use, is reviewed, with particular regard given to the effects of microstructural constituents.
Robust confidence annotation and rejection for continuous speech recognition (2002)

We are looking for confidence scoring techniques that perform well on a broad variety of tasks. Our main focus is on word-level error rejection, but most results apply to other scenarios as well. A variation of the normalized cross entropy that is adapted to that purpose is introduced. It is successfully used to automatically select features and optimize the word-level confidence measure on several test sets. Sentence-level confidence geared toward the rejection of out-of-grammar utterances is also investigated. The combination of a word graph based technique and the acoustic score shows excellent performance across all the tasks we considered.
Reducing the Peak to Average Power Ratio of LDS-OFDM signals (2010)

Low Density Signature-Orthogonal Frequency Division Multiplexing (LDS-OFDM) has been introduced recently as an efficient multiple access technique. High Peak to Average Power Ratio (PAPR) is an important obstacle to multicarrier communication systems. This paper concentrates on the PAPR investigation and its reduction for LDS-OFDM signals. Specifically, we will investigate the impact of subcarrier allocation schemes and the phases of the signatures on the PAPR of LDS-OFDM signals. Firstly, the PAPR of LDS-OFDM with conventional signatures is investigated. Then we propose two methods for PAPR reduction; Newman phases and DFT pre-coding. The former method is simple and doesn't imply changes in the system structure while the DFT pre-coding implies a modification in the system. Simulation results show that using Newman phases considerably reduces the PAPR of LDS-OFDM. Further PAPR reduction is achieved using DFT pre-coded LDS-OFDM on the cost of higher complexity.
Hierarchical honeycomb auxetic metamaterials (2015)

Abstract Most conventional materials expand in transverse directions when they are compressed uniaxially resulting in the familiar positive Poisson’s ratio. Here we develop a new class of two dimensional (2D) metamaterials with negative Poisson’s ratio that contract in transverse directions under uniaxial compressive loads leading to auxeticity. This is achieved through mechanical instabilities (i.e., buckling) introduced by structural hierarchy and retained over a wide range of applied compression. This unusual behavior is demonstrated experimentally and analyzed computationally. The work provides new insights into the role of structural organization and hierarchy in designing 2D auxetic metamaterials and new opportunities for developing energy absorbing materials, tunable membrane filters and acoustic dampeners.
Static and dynamic analysis of call chains in java (2004)

This work presents a parameterized framework for static and dynamic analysis of call chains in Java components. Such analyses have a wide range of uses in tools for software understanding and testing. We also describe a test coverage tool built with these analyses and the use of the tool on a real-world test suite. Our experiments evaluate the exact precision of several instances of the framework and provide a novel approach for estimating the limits of class analysis technology for computing precise call chains.
Renal AA-amyloidosis in intravenous drug users – a role for HIV-infection? (2012)

AA-amyloidosis was the predominant cause of progressive renal disease in the last 10 years in patients with IVDU. The highest rate of AA-amyloidosis observed was seen in HIV infected patients with IVDU. We speculate that chronic HIV-infection as well as the associated immunosuppression might promote development of AA-amyloidosis by increasing frequency and duration of infections acquired by IVDU.
Snoring: Clinical Implications and Treatment (1986)

Snoring has only recently come under wide study. Recent research has established the close relationship of severe snoring to sleep apnea in terms of the obstructive pathophysiology. Snoring tends to increase in severity over time and may progress to sleep apnea. Severe snoring may be associated with pulmonary and systemic hypertension, secondary polycythemia, and cardiac arrhythmias.
Foam pads properties and their effects on posturography in participants of different weight (2015)

The type of foam pads used in posturography is indeed important. Our study shows that the samples with a higher modulus of elasticity produced the largest change in postural sway velocity during quiet stance. The results suggest that foam pads used for static computerised posturography should 1) possess a higher modulus of elasticity and 2) show linear deformation properties matched to the participants' weight.
Lysosomes and Fas-mediated liver cell death (2007)

A number of studies, mostly performed ex vivo, suggest that lysosomes are involved in apoptosis as a result of a release of their cathepsins into the cytosol. These enzymes could then contribute to the permeabilization of the outer mitochondrial membrane; they could also activate effector caspases. The present study aims at testing whether the membrane of liver lysosomes is disrupted during Fas-mediated cell death of hepatocytes in vivo, a process implicated in several liver pathologies. Apoptosis was induced by injecting mice with aFas (anti-Fas antibody). The state of lysosomes was assessed by determining the proportion of lysosomal enzymes (beta-galactosidase, beta-glucuronidase, cathepsin C and cathepsin B) present in homogenate supernatants, devoid of intact lysosomes, and by analysing the behaviour in differential and isopycnic centrifugation of beta-galactosidase. Apoptosis was monitored by measuring caspase 3 activity (DEVDase) and the release of sulfite cytochrome c reductase, an enzyme located in the mitochondrial intermembrane space. Results show that an injection of 10 microg of aFas causes a rapid and large increase in DEVDase activity and in unsedimentable sulfite cytochrome c reductase. This modifies neither the proportion of unsedimentable lysosomal enzyme in the homogenates nor the behaviour of lysosomes in centrifugation. Experiments performed with a lower dose of aFas (5 microg) indicate that unsedimentable lysosomal hydrolase activity increases in the homogenate after injection but with a marked delay with respect to the increase in DEVDase activity and in unsedimentable sulfite cytochrome c reductase. Comparative experiments ex vivo performed with Jurkat cells show an increase in unsedimentable lysosomal hydrolases, but much later than caspase 3 activation, and a release of dipeptidyl peptidase III and DEVDase into culture medium. It is proposed that the weakening of lysosomes observed after aFas treatment in vivo and ex vivo results from a necrotic process that takes place late after initiation of apoptosis.
Biomedical Applications of Photochemistry (2010)

Photochemistry is the study of photochemical reactions between light and molecules. Recently, there have been increasing interests in using photochemical reactions in the fields of biomaterials and tissue engineering. This work revisits the components and mechanisms of photochemistry and reviews biomedical applications of photochemistry in various disciplines, including oncology, molecular biology, and biosurgery, with particular emphasis on tissue engineering. Finally, potential toxicities and research opportunities in this field are discussed.
Multicarrier Passive Inter-Modulation Prediction from 2-Carrier Measurements (2013)

Passive inter-modulation products between transmitted signals may prevent the correct operation of satellite receivers using the same antenna. In many cases, these passive products do not obey the classical rule of 3 dB/dB slope as a function of carrier input power and they cannot be modeled using the classical theory based on polynomials. This has prevented the exact computation of carrier to inter-modulation ratio in multicarrier conditions from 2-carrier measurements. This has led to the use of higher than necessary margins. We present non-analytic models that generate non-integer inter-modulation slopes identical to that obtained in measurements and permit to predict multicarrier results.
Developing Active Sensor Networks with Micro Mobile Robots: Distributed Node Localization. (2006)

A new sensor network architecture called active sensor network (ASN) is proposed in this paper. By integrating multiple small, sensor network-friendly mobile robots into a traditional sensor network, a closed-loop, dynamic, adaptive sensor network is formed. Such sensor networks have the following merits: adaptivity, self healing, responsiveness and longer lifetime. This paper focuses on the distributed sensor node localization using multiple mobile robots. A potentialbased robot area partition algorithm and a localization algorithm are developed. Simulation results verify the proposed algorithms.