gebhart/patrepeval huggingface dataset:
Substituent effects on the solution conformation of rifamycin S (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstituent effects on the solution conformation of rifamycin SM. F. Dampier, C. W. Chen, and H. W. Whitlock Jr.Cite this: J. Am. Chem. Soc. 1976, 98, 22, 7064–7069Publication Date (Print):October 1, 1976Publication History Published online1 May 2002Published inissue 1 October 1976https://pubs.acs.org/doi/10.1021/ja00438a055https://doi.org/10.1021/ja00438a055research-articleACS PublicationsRequest reuse permissionsArticle Views343Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Detection, differentiation, and identification of botulinum neurotoxin serotypes C, CD, D, and DC by highly specific immunoassays and mass spectrometry (2016)

Botulinum neurotoxin (BoNT) serotypes C and D and their mosaic variants CD and DC cause severe cases of botulism in animal husbandry and wildlife. Epidemiological data on the exact serotype or toxin variant causing outbreaks are rarely available, mainly because of their high sequence identity and the lack of fast and specific screening tools to detect and differentiate the four similar toxins. To fill this gap, we developed four highly specific sandwich enzyme-linked immunosorbent assays (ELISAs) able to detect and differentiate botulinum neurotoxins type BoNT/C, D, CD, and DC based on four distinct combinations of specific monoclonal antibodies targeting both conserved and divergent subdomains of the four toxins. Here, highly sensitive detection with detection limits between 2 and 24 pg mL(-1) was achieved. The ELISAs were extensively validated and results were compared with data obtained by quantitative real-time PCR using a panel of Clostridium botulinum strains, real sample materials from veterinary botulism outbreaks, and non-BoNT-producing Clostridia. Additionally, in order to verify the results obtained by ELISA screening, the new monoclonal antibodies were used for BoNT enrichment and subsequent detection (i) on a functional level by endopeptidase mass spectrometry (Endopep-MS) assays and (ii) on a protein sequence level by LC-MS/MS spectrometry. Based on all technical information gathered in the validation study, the four differentiating ELISAs turned out to be highly reliable screening tools for the rapid analysis of veterinary botulism cases and should aid future field investigations of botulism outbreaks and the acquisition of epidemiological data.
An improved technique for immunofixation of electrophoretograms. (1978)

Immunofixation coupled with electrophoresis is winning favor as an easy, sensitive, and versatile method for detecting specific protein fractions. Here, we discuss problems which appear when antisera are applied onto the electrophoretic medium (Cellogel or cellulose acetate). Longitudinal slits can be made in the electrophoretic medium without worsening resolution. Such longitudinally slitted electrophoretic membranes can be used in electrophoresis and specific antisera applied simply by pipetting them onto the electrophoretograms. The method has been used successfully in typing immunoglobulins.
Kinetic and spectral studies on the redox forms of methanol dehydrogenase from <i>Hyphomicrobium</i> X (1988)

Several reaction rate constants in the catalytic cycle of methanol dehydrogenase (EC 1.1.99.8) in vitro were determined with stopped-flow spectrophotometry. The studies revealed that the high pH required for adequate activity of the enzyme is related to the strong pH dependency of the oxidation rates of the reduced and semiquinone enzyme forms, MDHred and MDHsem, with the artificial electron acceptor Wurster's blue. The rate-limiting step in the catalytic cycle is associated with the conversion of oxidized enzyme-substrate complex (MDHox.S) into reduced enzyme (MDHred) and product. The effect of activator (ammonium salts) was also confined to this step, but even saturating concentrations were unable to remove the limitation completely. Making use of the large deuterium isotope effect associated with substrate oxidation in the absence of activator, the transient MDHox.C2H3OH complex could be isolated and its slow decomposition into MDHred and formaldehyde could be demonstrated. Further evidence is presented to support the view that the different absorption spectra observed originate from genuine redox forms of methanol dehydrogenase with different redox states of the cofactor PQQ and not from enzyme-electron acceptor complexes. The results confirm and extend our original view on the mechanism of action and contradict the mechanism proposed by others [Parkes, C. & Abeles, R. H. (1984) Biochemistry 23, 6355-6363].
Detection of circulating adhesion molecules ICAM-1, VCAM-1 and E-selectin in Wegener's granulomatosis, systemic lupus erythematosus and chronic renal failure. (1995)

The adhesion molecules ICAM-1, VCAM-1 and E-Selectin are regulated by proinflammatory cytokines and play an important role in the binding and activation of leukocytes in inflammatory diseases. This study measured the serum concentrations of circulating adhesion molecules (cICAM-1, cVCAM-1, cE-Selectin) by sandwich ELISA in systemic vasculitis with renal involvement (Wegener's granulomatosis WG, n = 25; systemic lupus erythematosus SLE, n = 50) in comparison to chronic glomerulonephritis (n = 10), stable renal allograft function and end-stage renal disease (CAPD/hemodialysis, each n = 10). Both cICAM-1 and cVCAM-1 levels, but not cE-Selectin, were significantly increased (p < 0.001) in active WG compared to healthy controls. cICAM-1, not cVCAM-1 differed significantly between active and inactive WG. Only cVCAM-1 was significantly elevated in active/inactive SLE (p < 0.01). WG with rapidly progressive glomerulonephritis had significantly raised levels of cICAM-1 and cVCAM-1, but not cE-Selectin, compared to controls (p < 0.005). In lupus nephritis only cVCAM-1 was significantly elevated (p < 0.01). cICAM-1 and cVCAM-1 levels were significantly raised in WG patients, that were hemodialysed and in patients with hemodialysis because of different reasons when compared to controls. However, cVCAM-1 but not cICAM-1 was significantly higher in WG with HD than without HD. Patients with chronic glomerulonephritis, renal allografts or CAPD also had significantly raised cVCAM-1 concentrations. These data suggest, that levels of circulating adhesion molecules might reflect different pathophysiologic processes in systemic vasculitides and endothelial/immune activation in non-inflammatory renal diseases.(ABSTRACT TRUNCATED AT 250 WORDS)
MAST suit update (1984)

THE MAST suit has become a standard component of the prehospitalization armamentarium.<sup>1</sup>Other terms in the medical literature that have been used to refer to essentially the same device include medical antishock trousers, military antishock trousers, antishock garments, G-suits, anti-shock air pants, external counterpressure devices, pneumatic trousers, counterpressure suits, circumferential pneumatic compression devices, and pneumatic antishock garments. MAST suit is the most popular term; it is often used to refer to all of the aforementioned devices, although it is a trade name. This article critiques the antishock trousers made by all manufacturers. When we allude to MAST suits we are not singling out one manufacturer but referring to the generic product. The MAST suit consists of a pair of trousers with three inflatable bladders held in place by Velcro fasteners or zippers. It resembles a large blood pressure cuff tailored into a pair of pants. The suit consists of
Analog Circuit for Timing Measurements With Large Area SiPMs Coupled to LYSO Crystals (2013)

The most common method for time pick-off from signals coming from SiPMs coupled to scintillator crystals in PET applications is the Leading Edge Triggering. In this work we propose a new filtering scheme to be applied before the discriminator. It implements a type of baseline compensation aimed at the reduction of the time jitter due to the dark counts of the detector and is based on a simple analog filter, which is well suited for an ASIC implementation. We describe a circuit, built with discrete components, that implements the filter. Finally we report on the Coincidence Resolving Time measurements performed coupling the circuit to the real detector, composed of a SiPM built at FBK and a LYSO crystal, for the detection of 511 keV photons. The results obtained are promising, showing that the method is quite effective in reducing the impact of the detector noise on the timing performance of the system.
A hybrid full-wave analysis of via-hole grounds using finite-difference and finite-element time-domain methods (1997)

A hybrid full-wave analysis using finite-difference time-domain (FDTD) and finite-element time-domain (FETD) methods is developed to analyze locally arbitrarily shaped microwave structures. This hybrid method employs the standard FDTD method with super-absorbing Mur's first-order absorbing-boundary condition (ABC) and the FETD method using the second-order vector prism elements. An interpolation scheme is proposed for communicating between the FDTD and FETD fields, which will not require the effort of fitting the FETD mesh to the FDTD cells in the interface region. This method is applied to calculate the scattering parameters of single and multiple cylindrical via holes in a microstrip structure. Applying FETD to the via-hole grounds and FDTD to the remaining region preserves the advantages of both FETD flexibility and FDTD efficiency. A comparison of the results with the mode-matching data and the FDTD staircasing data verifies the accuracy of the proposed method.
The Gαi-GIV binding interface is a druggable protein-protein interaction (2017)

Heterotrimeric G proteins are usually activated by the guanine-nucleotide exchange factor (GEF) activity of GPCRs. However, some non-receptor proteins are also GEFs. GIV (a.k.a Girdin) was the first non-receptor protein for which the GEF activity was ascribed to a well-defined protein sequence that directly binds Gαi. GIV expression promotes metastasis and disruption of its binding to Gαi blunts the pro-metastatic behavior of cancer cells. Although this suggests that inhibition of the Gαi-GIV interaction is a promising therapeutic strategy, protein-protein interactions (PPIs) are considered poorly "druggable" targets requiring case-by-case validation. Here, we set out to investigate whether Gαi-GIV is a druggable PPI. We tested a collection of >1,000 compounds on the Gαi-GIV PPI by in silico ligand screening and separately by a chemical high-throughput screening (HTS) assay. Two hits, ATA and NF023, obtained in both screens were confirmed in secondary HTS and low-throughput assays. The binding site of NF023, identified by NMR spectroscopy and biochemical assays, overlaps with the Gαi-GIV interface. Importantly, NF023 did not disrupt Gαi-Gβγ binding, indicating its specificity toward Gαi-GIV. This work establishes the Gαi-GIV PPI as a druggable target and sets the conceptual and technical framework for the discovery of novel inhibitors of this PPI.
Aerobic Oxygenation of Olefinic Compounds into the Corresponding α-Hydroxy Ketones Using the Catalyst System of OsO4 and Ni(II) Complex (1991)

Abstract In the presence of the catalyst system of OsO4 and bis(3-methyl-2,4-pentanedionato)nickel(II), various olefinic compounds are directly converted into the corresponding α-hydroxy ketones in good yields with molecular oxygen and an aldehyde.
Relation Between Olfactory Threshold Concentration and Pyruvic Acid Content of Onion Juice (1962)

SUMMARY A highly significant correlation (r =−0.97) exists between the amount of enzymatically developed pyruvic acid present in the juice of comminuted onion and the olfactory threshold concentration of the juice. The correlation indicates that determination of pyruvic acid in freshly prepared onion juice constitutes a fairly reliable, simple, and convenient method of estimating at least one aspect of onion flavor.
Source and system features for speaker recognition using AANN models (2002)

We study the effectiveness of the features extracted from the source and system components of the speech production process for the purpose of speaker recognition. The source and system components are derived using linear prediction (LP) analysis of short segments of speech. The source component is the LP residual derived from the signal, and the system component is a set of weighted linear prediction cepstral coefficients. The features are captured implicitly by a feedforward autoassociative neural network (AANN). Two separate speaker models are derived by training two AANN models using feature vectors corresponding to source and system components. A speaker recognition system for 20 speakers is built and tested using both the models to evaluate the performance of source and system features. The study demonstrates the complementary nature of the two components.
The pharmacological properties of Y‐23684, a benzodiazepine receptor partial agonist (1994)

1. The pharmacological properties of a benzodiazepine receptor (BZR) partial agonist, Y-23684 were investigated in comparison with those of diazepam, a conventional BZR full agonist. 2. Y-23684 and diazepam showed high and selective affinity for the BZR with Ki values of 41 and 5.8 nM, respectively. 3. In contrast to diazepam, variability was noted in the anticonvulsive potency of Y-23684 depending on convulsants (bicuculline, pentylenetetrazol and maximal electrical shock). Y-23684 produced the most potent protective effect against bicuculline in rats and mice with ED50S of 1.3 and 1.2 mg kg-1, respectively. 4. In rat conflict models (Geller-Seifter and water-lick tests), Y-23684 produced an antipunishment action at doses 2-4 times lower than diazepam. In contrast to diazepam, Y-23684 did not affect unpunished responding up to 50 mg kg-1 in the Geller-Seifter test. 5. In other rat models of anxiety (social interaction and elevated plus-maze tests), Y-23684 was as efficacious as and ten fold more potent than diazepam. In a mouse model of anxiety (exploration (light/dark box) test), Y-23684 was as efficacious and two fold less potent as diazepam. In these paradigms, Y-23684 showed a selective anxiolytic profile over a wide dose-range without loss of efficacy and sedative action. 6. The impairment of motor coordination (rotarod) and potentiation of CNS depressants (ethanol and hexobarbitone) by Y-23684 was much weaker than that of diazepam. 7. These results suggest that Y-23684 would be a potent and selective anxiolytic agent in man with less side-effects than conventional BZ-anxiolytics.
Leaf Variation among Cannabis Species from a Controlled Garden (1980)

The genus Cannabis has had a long association with man and contains considerable variation in growth form, achene size, and chemical content. The variation has taxonomically been variously interpreted. Some maintain the genus is polytypic with at least three species (Emboden, 1974; Schultes et al., 1974). Others (Small and Cronquist, 1976) believe that it is properly viewed as monotypic: i.e. limited to C. sativa L. They do, however, recognize several infraspecific varieties. Historical aspects and rationale for the different treatments are amply covered in these papers. My work on wood anatomy (Anderson, 1974) supported the polytypic generic concept. A controlled garden (2.6 acres) is maintained at the University of Mississippi by the School of Pharmacy for the National Institute on Drug Abuse where collections from throughout the world are propagated. In 1976, 1 visited the facility several times to obtain additional vouchered wood samples from plants grown in a uniform garden. While gathering the Cannabis samples, I became aware of differences in leaf morphology. Variation in garden-grown plants has already been noted by Quimby et al. (1973), but they did not quantify their data. This report analyzes leaf form in Cannabis and compares the variation in relation to the alternative taxonomie treatments.
Packetization of 3D progressive meshes for streaming over lossy networks (2006)

In this paper, we consider the problem of packetizing progressive 3D geometry models for streaming over a lossy network. We adopt a graph-theoretic approach to model packetization of progressive 3D models, with the goal of minimizing dependencies among packets. We show that this packetization problem is strongly NP-complete, and we propose two packing heuristics. Our experiments show that both heuristics perform better than the naive method. In particular, the greedy packing heuristic produces significant improvement in the number of rendered nodes when the network is lossy.
[Humoral immunization and cell-mediated immunization evoked by HBsAg and B7-2 Ag coexpression recombinant adenovirus vector]. (2001)

This could be a novel strategy for a development of both preventive and therapeutic vaccines against HBV infection. The recombinant adenovirus vector is an effective and safety vector system suitable to the experiments of gene immunization and gene therapy for incurable diseases.
Optimization of the Synthesis of <I>α</I>-MoO<SUB>3</SUB> Nanoribbons and Hydrodesulfurization (HDS) Catalyst Test (2007)

An optimized process for synthesis of alpha-MoO3 nanoribbons characterized by uniform morphology and composition was carried out. The optimized process turned out to be the aging of a precursor of an aqueous solution of ammonium heptamolybdate for a week under constant stirring at 333 K; followed by hydrothermal treatment for 36 h up to 48 h at 473 K. The dimensions of the nanoribbons were between 5 and 10 microm in length and a width between 100 and 600 nm. The thickness was between 60 and 200 nm. This material was tested for hydrodesulfurization (HDS) of dibenzothiophene (DBT) by in situ activation and showed its catalytic activities to be similar to those of unsupported MoS2 catalysts. The structure and morphology of these materials was characterized by analytical transmission electron microscopy, scanning electron microscopy, and X-ray diffraction using the Rietveld method to determine the quantitative crystallographic phases. A chemical semiquantitative analysis was carried out by energy dispersive spectroscopy and a qualitative analysis was carried out by electron energy loss spectroscopy.
Investigation of a unified LTCC-based micromachining and packaging platform for high density/multifunctional microsystem integration (2012)

3D system-in-package has recently been considered a major enabler for high density and heterogeneous microsystem integration. We hereby proposed the concept of a unified micromachining and packaging platform based on LTCC (low temperature cofired ceramic) material system and process, which is implemented by first enhancing an existing LTCC hybrid IC fabrication line and then integrating different LTCC micromachining process modules one by one. Hence, the unified process flow can be accomplished within just one single package-test house. The platform has been capable of micromachining basic 3D MEMS (micro electromechanical system) microstructures into LTCC laminates and using them as a packaging substrate for mounting IC/MEMS from other process platforms, realizing self-contained and versatile microsystems of high density. The 3D microstructures formation process consisting of green tape machining, lamination and cofiring are demonstrated. The designing, analysis and fabricated samples of various micro functional structure enabled by the platform are illustrated, including embedded cooling microchannels (capable of lowering substrate temperature by more than 50K), microaccelerometer for harsh environment, micro Pirani gauge for in-situ vacuum level monitoring and THz (tera hertz) vacuum microelectronic devices. Samples of overall packaged MEMS and IC chips with micromachined LTCC substrate are displayed, showing ultra-low leakage (<; 5×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-11</sup> Pa·m <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> /s) vacuum packaging capability and significantly enhanced device performance. In addition, the platform has demonstrated the potential of stacking several laminates with mounted chips into a 3D frame-like microsystem. In comparison, 3D integration purely based on Si micromachining, e.g. anodic-bonding based in-situ wafer encapsulation, may only support a very limited spectrum of devices/materials and integration density and is somehow too expensive for many MEMS researchers.
Computational Physics Group (1970)

While I admire Mr B McNamara's enthusiasm for the Computational Physics Group, I cannot share it. The first meeting (see Physics Bulletin October 1969 p439) was enjoyable both for the hospitality of the Culham Laboratory and for the wide range of physics available for discussion.
Predictive Simulation and Benchmarking of Si and Ge pMOS FinFETs for Future CMOS Technology (2014)

In this paper, we study and compare Si versus Ge pMOS FinFETs at advanced node dimensions using ensemble Monte Carlo simulations. It is found that due to large external resistance, lack of stressing methods, smaller bandgap, larger dielectric constant, and increased variability that in the absence of major innovation, Ge is not an ideal candidate for channel replacement material of pMOS in future CMOS technology generation FinFETs. In order for Ge to compete with Si, it would at a minimum require a stressing mechanism and improved contact resistance, but leakage and variability would still be a concern for low-power applications.
Inclination Parameter Estimation for Manipulator and Humanoid Robot Links (2011)

A novel approach of dynamic, non-contact measurement of inclination parameters for robotics applications using the Vestibular Dynamic Inclinometer (VDI) is proposed in the paper. The gravity-invariant Vestibular Dynamic Inclinometer (VDI) is a sensor that consists of symmetrically placed two dual-axis linear accelerometers and one single-axis gyroscope. The deployment of the non-contact sensor is strategic and need not be exactly at the joints. The sensor is also able to estimate the inclination parameters of the base link and the acceleration of the surface of contact. The inclination parameters — base angle, joint angles, angular velocity and angular acceleration, are independent of integration errors/drift.