gebhart/patrepeval huggingface dataset:
Specific Function of DNA Ligase I in Simian Virus 40 DNA Replication by Human Cell-free Extracts Is Mediated by the Amino-terminal Non-catalytic Domain (1997)

The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is believed to be due to DNA ligase I. This enzyme is composed of a 78-kDa carboxyl-terminal catalytic domain and a 24-kDa amino-terminal region that is not required for ligation activity in vitro. Extracts of the human cell line 46BR.1G1, in which DNA ligase I is mutationally altered, supported aberrant in vitro SV40 DNA replication; the joining of Okazaki fragments was defective, and unligated intermediates were unstable. Human DNA ligase I, but not DNA ligase III or bacteriophage T4 DNA ligase, complemented both defects in 46BR.1G1 extracts. The catalytic domain of DNA ligase I was 10-fold less effective in complementation experiments than the full-length protein, indicating that the amino-terminal region of the enzyme is required for efficient lagging strand DNA replication. Moreover, in vitro SV40 DNA replication in normal human cell extracts was inhibited by an excess of either full-length DNA ligase I or the amino-terminal region of the protein, but not by the catalytic domain. This inhibition may be mediated by the interaction of the amino-terminal region of DNA ligase I with other replication proteins.
Characterization of the promoter elements required for hepatic and intestinal transcription of the human apoB gene: definition of the DNA-binding site of a tissue-specific transcriptional factor. (1990)

The promoter elements important for intestinal and hepatic transcription of the human apoB gene have been localized downstream of nucleotide -150. Footprinting analysis using hepatic nuclear extracts identified four protected regions, -124 to -100, -97 to -93, -86 to -33, and +33 to +52. Gel electrophoretic mobility shift assays showed that multiple factors interact with the apoB sequence -86 to -33, while the region -88 to -61 binds a single nuclear factor. Methylation interference analysis and nucleotide substitution mutagenesis identified the binding site of the factor between residues -78 and -68. Binding competition experiments indicate that this factor recognizes the regulatory elements of other liver-specific genes.
Effects of supplementing organic microminerals and methionine during the rearing phase of replacement gilts on lameness, growth, and body composition1 (2018)

Lameness is a primary reason for culling and mortality within a sow herd. This study evaluated the impact of feeding organic trace minerals and methionine (Met) to growing gilts (134 d) on lameness, performance, body composition and claw health (to first parity), productivity (to second parity), and reproductive performance through 2 parities. Young gilts (28.8 ± 8.8 kg of body weight [BW], n = 360) were BW blocked (10 gilts/pen) and randomly allotted to 1 of 4 dietary treatments: control (CON, basal diet); CON plus organic minerals (MIN, at 10, 20, and 50 mg/kg of Cu, Mn, and Zn, respectively; Aplomotec Plus, Tecnología & Vitaminas, S.L, Alforja, Spain); additional Met (MET, at 102% Met: Lys); and MET plus MIN (MM). Feed was provided ad libitum. Lameness, BW, and body composition were measured 7 times during rearing, at gilt service, day 109 of gestation, and first weaning. Gilts fed the MM diet had lower average daily feed intake (5.1%) and final BW (2.1%) than CON gilts (P < 0.05), whereas MIN and MET were intermediate and not different from each other. Similarly, final backfat (BF) was greatest in CON (P < 0.05), whereas CON and MIN increased final loin depth compared with MM (P < 0.05) with MET not being different. During rearing, 7.7% of all gilts presented lameness, which appeared between 106.8 and 129.7 kg BW confidence interval. Gilts that had been or were lame had reduced BW and average daily gain compared with never lame gilts (P < 0.05). Lameness during rearing was highest (P < 0.01) in gilts fed CON diet (14.8%), with no differences amongst MIN (2.0%), MET (5.3%), or MM (6.5%). In the sow herd, 21% of sows showed lameness and 24% of those were associated with claw lesions. At weaning, gilts fed CON diet had highest (P < 0.01) prevalence of lameness (20.8%) with no differences amongst MIN (6.5%), MET (11.1%), or MM (7.6%). Over the first 2 parities, 27.3% of gilts were culled. On farm, lameness was associated with 0.7 more stillborn piglets (P < 0.10), 1 mm more BF loss in first lactation (P < 0.05), and increased weaning-to-estrus by 3 d (P < 0.05). In conclusion, lameness during rearing was decreased by supplementing organic trace minerals, methionine, and their combination, which also reduced lameness during lactation.
Translation: In retrospect and prospect (2001)

This review is occasioned by the fact that the problem of translation, which has simmered on the biological sidelines for the last 40 years, is about to erupt center stage--thanks to the recent spectacular advances in ribosome structure. This most complex, beautiful, and fascinating of cellular mechanisms, the translation apparatus, is also the most important. Translation not only defines gene expression, but it is the sine qua non without which modern (protein-based) cells would not have come into existence. Yet from the start, the problem of translation has been misunderstood--a reflection of the molecular perspective that dominated Biology of the last century. In that the our conception of translation will play a significant role in creating the structure that is 21st century Biology, it is critical that our current (and fundamentally flawed) view of translation be understood for what it is and be reformulated to become an all-embracing perspective about which 21st century Biology can develop. Therefore, the present review is both a retrospective and a plea to biologists to establish a new evolutionary, RNA-World-centered concept of translation. What is needed is an evolutionarily oriented perspective that, first and foremost, focuses on the nature (and origin) of a primitive translation apparatus, the apparatus that transformed an ancient evolutionary era of nucleic acid life, the RNA World, into the world of modern cells.
High-frequency-variation speed control of spindle motor for chatter vibration suppression in NC machine tools (2014)

This paper proposes a spindle motor control method for self-excited chatter vibration suppression in numerical control (NC) machine tools. In conventional NC machine tools, spindle speed is set to constant value during machining, and the spindle speed is determined according to analysis or operator's experience. The proposed method reduces self-excited chatter vibration by varying spindle speed with high frequency during machining. This method can also suppress the disturbance derived from cutting resistance by repetitive control, and achieve spindle speed variation with high frequency by perfect tracking control. Finally, we show the advantages of the proposed method by simulations and experiments.
Guayule Rubber: Vulcanization Properties of Gum and Filled Stocks (1977)

Abstract Guayule rubber has been used both in the resinous and deresinated form for more than sixty years during periods of either scarcity or high prices of hevea rubber. During this time little or no efforts have been made to standardize the individual processes to a point where the products had specifications similar to the “Technically Specified Rubbers”. This lack of specifications consequently left users of the guayule rubbers with various opinions of the capabilities of this type of natural rubber in their commercial products. It was generally believed that the rubber was only good for low-grade products or where guayule was only used as a tackifier for other rubbers. Recent interest in the utilization of arid regions and the increasing need for less dependence on foreign sources has led the Mexican government to develop a modern guayule product. It was the purpose of this study to evaluate Mexican guayule rubber in cooperation with CIQA in an effort to determine processing and vulcanization characteristics of the products prepared in their pilot plant in Saltillo, Mexico.
Practical Real‐Time Lens‐Flare Rendering (2013)

Abstract We present a practical real‐time approach for rendering lens‐flare effects. While previous work employed costly ray tracing or complex polynomial expressions, we present a coarser, but also significantly faster solution. Our method is based on a first‐order approximation of the ray transfer in an optical system, which allows us to derive a matrix that maps lens flare‐producing light rays directly to the sensor. The resulting approach is easy to implement and produces physically‐plausible images at high framerates on standard off‐the‐shelf graphics hardware.
mixOmics: An R package for ‘omics feature selection and multiple data integration (2017)

The advent of high throughput technologies has led to a wealth of publicly available 'omics data coming from different sources, such as transcriptomics, proteomics, metabolomics. Combining such large-scale biological data sets can lead to the discovery of important biological insights, provided that relevant information can be extracted in a holistic manner. Current statistical approaches have been focusing on identifying small subsets of molecules (a 'molecular signature') to explain or predict biological conditions, but mainly for a single type of 'omics. In addition, commonly used methods are univariate and consider each biological feature independently. We introduce mixOmics, an R package dedicated to the multivariate analysis of biological data sets with a specific focus on data exploration, dimension reduction and visualisation. By adopting a systems biology approach, the toolkit provides a wide range of methods that statistically integrate several data sets at once to probe relationships between heterogeneous 'omics data sets. Our recent methods extend Projection to Latent Structure (PLS) models for discriminant analysis, for data integration across multiple 'omics data or across independent studies, and for the identification of molecular signatures. We illustrate our latest mixOmics integrative frameworks for the multivariate analyses of 'omics data available from the package.
Self-energized wireless pressure sensor using energy extraction from injection mold pressure differential (2003)

With the prolific use of sensors for manufacturing process monitoring and the growing demand for system integration, the issue of packaging and installation has assumed an increasingly central role. This paper presents the design of a self-energized pressure sensor that extracts energy from the pressure differential of the polymer melt during the injection molding process. A piezoelectric element is used as the energy converter to convert the high melt pressure into proportional electrical charges, which in turn, actuate an ultrasound signal through a miniature energy switch. Based on predetermined energy threshold values, the actuator generates a train of ultrasound pulses, which represent the continuous melt pressure in a digitized form. The ultrasound pulses propagate wirelessly through the mold steel and are detected by a remotely located signal receiver. Through multiplication of the number of pulses with the energy threshold values, the polymer melt pressure profile is reconstructed. To enable a self-energized sensor design, an analytical study has been conducted to establish a quantitative relationship between the polymer melt pressure and the energy that can be extracted through the use of a piezoelectric converter.
Biophotonics of Native Silk Fibrils (2018)

Native silk fibroin (NSF) is a unique biomaterial with extraordinary mechanical and biochemical properties. These key characteristics are directly associated with the physical transformation of unstructured, soluble NSF into highly organized nano- and microscale fibrils rich in β-sheet content. Here, it is shown that this NSF fibrillation process is accompanied by the development of intrinsic fluorescence in the visible range, upon near-UV excitation, a phenomenon that has not been investigated in detail to date. Here, the optical and fluorescence characteristics of NSF fibrils are probed and a route for potential applications in the field of self-assembled optically active biomaterials and systems is explored. In particular, it is demonstrated that NSF can be structured into autofluorescent microcapsules with a controllable level of β-sheet content and fluorescence properties. Furthermore, a facile and efficient fabrication route that permits arbitrary patterns of NSF microcapsules to be deposited on substrates under ambient conditions is shown. The resulting fluorescent NSF patterns display a high level of photostability. These results demonstrate the potential of using native silk as a new class of biocompatible photonic material.
A Recipe Based On-line Food Store (1999)

In this paper we present a recommender system design for recipe based on-line food shopping. Our system differs in two major ways from existing system. First we use an editor that labels clusters of users, such as meat lovers and vegetarians; based on what recipes they have chosen. Secondly, these clusters are available to users, so they can not only choose recipes based on their own user group but also navigate among other user groups.
Paper stiffness monitoring using laser ultrasonics (2002)

The use of a laser ultrasonics method to induce and detect Lamb waves in paper was demonstrated. A pulsed Nd:YAG laser was used for Lamb wave excitation. Detection of out-of-plane and in-plane surface motions was accomplished using a CW Ar:ion laser and appropriate heterodyne interferometric systems. Two propagation modes, a fast mode and a slow mode, were observed with substantially different velocities, amplitudes, and frequency contents. They were found to correspond to the fundamental dilatational (S/sub 0/) and bending (A/sub 0/) modes for Lamb waves, respectively. Velocity measurements gathered along paper machine direction and cross-machine direction for the S/sub 0/ mode were shown to be in very good agreement with velocities obtained using a pair of contact bimorph transducers. Results are presented for various commercial paper grades ranging from fine papers to paperboards. The rationale for contactless real-time stiffness monitoring of a moving paper web is discussed.
Synthesis and properties of new antiferroelectric liquid crystals with tetralin core (1996)

Abstract New antiferroelectric liquid crystals with tetralin core were synthesized. There is the limitation of the molecular length to exhibit SmCA* phase. The stability of SmCA* phase depends on the position and orientation of tetralin core.
Measurement of fiber grating properties using an interferometric and Fourier-transform-based technique (2002)

In conclusion, we have made approximate measurements of the characteristic fibre Bragg grating (FBG) properties that fit very well to the specified profiles. However, accuracy is lost in the measurements for FBGs with high reflectivities.
Integrin‐linked kinase production prevents anoikis in human mesenchymal stem cells (2007)

Human mesenchymal stem cells (hMSCs) were infected with an adenovirus expressing integrin-linked kinase (ILK) to understand the role of cell-ECM signal transduction cascades in suppressing anoikis. Survivability of ILK-infected hMSCs encapsulated in poly(ethylene glycol) (PEG) hydrogels, an anoikis-inducing environment, was sustained at 90% over 7 weeks, and survival was attributed to increased protein kinase B (PKB/Akt) activation. hMSCs encapsulated in RGD-modified hydrogels induced an upregulation in ILK production, PKB/Akt activation, and subsequent survival to the same extent of ILK-infected, encapsulated hMSCs. As negative controls, encapsulated hMSCs were infected with cyclization recombinase (a protein not associated with cell survival)-expressing virus, and uninfected hMSCs exhibited very little ILK production, PKB/Akt activation, and survival ( approximately 55% after 7 weeks). As a measure of cell-matrix interactions, vinculin was also quantified for the encapsulated hMSCs and found to be 30-fold greater for cells encapsulated in RGD-modified hydrogels and fivefold greater for ILK-infected hMSCs than controls, indicating that cell-material interactions are inducing the cell survivability of hMSCs encapsulated in RGD-modified hydrogels. In sum, ILK infection can support cell survival in the absence of matrix interactions and enable fundamental studies of three-dimensional cell function in response to extrinsic signals, independently of matrix-ligand interactions.
Encrypted Set Intersection Protocol for Outsourced Datasets (2014)

Secure and efficient data storage and computation for an outsourced database is a primary concern for users, especially with the push for cloud computing that affords both compute and resource scalability. Among the diverse secure building blocks for secure analytical computations on outsourced databases, the encrypted set intersection operation extracts common sensitive information from datasets belonging to different users. In existing methods, each user holds their sensitive data and all users follow a secure protocol to perform set intersection. This approach is not applicable to the cloud platform, where data resides in the cloud platform in encrypted form and not at each user site. To address this limitation, in this paper, we design the Encrypted Set Intersection Protocol (ESIP) that allows server and users to perform collaborative operations to obtain the correct set intersection result without violating privacy of data contributed by each user at the server.
Synthesis, Crystal Structure, and Potentiometry of Pyridine-Containing Tetraaza Macrocyclic Ligands with Acetate Pendant Arms (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis, Crystal Structure, and Potentiometry of Pyridine-Containing Tetraaza Macrocyclic Ligands with Acetate Pendant ArmsWon D. Kim, Duane C. Hrncir, Garry E. Kiefer, and A. Dean SherryCite this: Inorg. Chem. 1995, 34, 8, 2225–2232Publication Date (Print):April 1, 1995Publication History Published online1 May 2002Published inissue 1 April 1995https://pubs.acs.org/doi/10.1021/ic00112a040https://doi.org/10.1021/ic00112a040research-articleACS PublicationsRequest reuse permissionsArticle Views638Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Phytosulphokine gene regulation during maize (Zea mays L.) reproduction* (2005)

The sulphated pentapeptide phytosulphokine (PSK) was identified as a substance that promotes cell division in low-density suspension cultures and has been implicated in various aspects of tissue differentiation in plants. The peptide is derived from PSK precursor proteins that are encoded by small gene families. The physiological roles of PSK are still not clearly defined and little is known about expression of members of the PSK precursor gene family in any plant species. In this study, highly regulated tissue and cell type-specific expression are described for four PSK genes from maize (Zea mays L.) in female and male gametophytes, and during seed development. ZmPSK1 and ZmPSK3 were specifically and differentially expressed in cells of female and male gametophytes and in female and male gametes. In anthers ZmPSK1 or ZmPSK3 transcripts were found, for example, at high levels in secretory tapetal cells which support developing microspores. ZmPSK1 mRNA was abundant in mature pollen including sperm cells. ZmPSK1 and ZmPSK3 transcripts were also detected in egg and central cells of the female gametophyte and ZmPSK1 mRNA was present in synergids, indicating that the PSK peptide probably plays a role during gametogenesis, pollen germination, and fertilization. In developing maize kernels all four ZmPSK genes were expressed, albeit with striking differences in their expression patterns. It is proposed here that PSK is required for numerous but defined processes during gametophyte and early sporophyte development. In general, PSK availability appears to be controlled through transcriptional regulation in a tissue and cell type-specific and development-dependent manner.
Dynamic behavior of simple magnetic hole systems (1990)

Experiments on a system of two magnetic holes (nonmagnetic microspheres in ferrofluid) subject to a rotating magnetic field show various types of behavior depending on the driving frequency. For two spheres (holes) mechanically bound together the stable rotation mode at low frequencies is replaced above a critical frequency by a mode with alternating rotation directions. This is described by simple nonlinear equations, and simulations show good quantitative agreement with the experiments. For two free spheres not bound together one observes a transition to a mode in which the spheres undergo both angular and radial motion around the center of mass of the system. Depending on the frequency and the anisotropy of the rotating magnetic field the motion passes through a sequence of states with sphere rotation mode-locked to the driving frequency at different ratios in good qualitative agreement with simulations. Adding a constant magnetic field normal to the plane causes the minimum sphere separation to increase from contact to a finite value. At high frequencies the spheres are influenced by an effective magnetic potential, giving rise to a static particle separation proportional to the normal component of the field.
Localization of glucagon-like peptide (GLP) immunoreactants in human gut and pancreas using light and electron microscopic immunocytochemistry. (1985)

The distribution of peptide immunoreactivities predicted from the sequence of the human preproglucagon gene in enteroglucagon (EG; glicentin-like immunoreactant-containing) cells of the human gut and A cells of the pancreas has been determined by light and electron microscopic immunocytochemistry. At light microscopy the application of peroxidase-antiperoxidase and immunogold-silver staining methods has revealed that glucagon-like peptide (GLP-1 and GLP-2) immunoreactivities coexist with a glicentin-related immunodeterminant in human colorectal EG cells and pancreatic A cells. Using single and double colloidal gold probe electron immunocytochemistry, we have been able to show the coexistence of glicentin, GLP-1, and GLP-2 immunoreactivities within single EG cell secretory granules. No morphologic segregation of the proglucagon immunoreactants was observed in EG cells of the colonic mucosa. In pancreatic A cells we have localized GLP-1, GLP-2, and glucagon-[16-29] immunoreactivities solely to the electron-dense core of the secretory granules, whereas glicentin-related immunoreactivity was restricted to the electron-lucent halo. The results obtained in the present study have shown that the peptide immunoreactivities predicted from cDNA sequencing of the human preproglucagon gene are indeed expressed in colorectal EG and pancreatic A cells. The topographical segregation of immunoreactivities in the A cell secretory granule shows that antigenic determinants derived from the C-terminal portion of proglucagon are stored with glucagon in the core of the secretory granule.