gebhart/patrepeval huggingface dataset:
Early Production of Type I Interferon during West Nile Virus Infection: Role for Lymphoid Tissues in IRF3-Independent Interferon Production (2007)

Infection of cells with flaviviruses in vitro is reduced by pretreatment with small amounts of type I interferon (IFN-alpha/beta). Similarly, pretreatment of animals with IFN and experiments using mice defective in IFN signaling have indicated a role for IFN in controlling flavivirus disease in vivo. These data, along with findings that flavivirus-infected cells block IFN signaling, suggest that flavivirus infection can trigger an IFN response. To investigate IFN gene induction by the very first cells infected during in vivo infection with the flavivirus West Nile virus (WNV), we infected mice with high-titer preparations of WNV virus-like particles (VLPs), which initiate viral genome replication in cells but fail to spread. These studies demonstrated a brisk production of IFN in vivo, with peak levels of over 1,000 units/ml detected in sera between 8 and 24 h after inoculation by either the intraperitoneal or footpad route. The IFN response was dependent on genome replication, and WNV genomes and WNV antigen-positive cells were readily detected in the popliteal lymph nodes (pLN) of VLP-inoculated mice. High levels of IFN mRNA transcripts and functional IFN were also produced in VLP-inoculated IFN regulatory factor 3 null (IRF3(-/-)) mice, indicating that IFN production was independent of the IRF3 pathways to IFN gene transcription, consistent with the IFN type produced (predominantly alpha).
Recent Progress of Vascular Graft Engineering in Japan (2004)

The development of a small-diameter vascular graft has long been awaited. This review covers research activities, achievements and progress on vascular engineering in Japan, which was conducted over the last decade. The article includes recently developed experimental scaffolds, biologically active artificial extracellular matrices (ECMs) or non-fouling synthetic coatings, cell sourcing including the autologous vascular cell type, endothelial progenitor cells and genetically-engineered, temporary endothelial-like cells. The discussions were presented from biomechanical, biomaterial, cellular and tissue aspects. Once the mechano-biological and biologically active extracellular milieus are established in a designed vascular graft, the functional, structural and mechanical tissue morphogenesis and adaptation of implanted vascular grafts may proceed with implantation duration, and the spatio-temporal tissue modulations at cytokine, cellular, ECM levels under physiological stress proceed to regenerate vascular tissue architecture. The ultimate solution to a small-diameter vascular graft should be realized by optimal combinations of these factors.
STEP-NC and function blocks for interoperable manufacturing (2006)

Interoperable manufacturing systems help manufacturing companies stay competitive in the environment of frequent and unpredictable market changes. An important part of a manufacturing system is computer numerically controlled (CNC) machine tools. Over the years, G-codes have been extensively used by CNC machine tools and are now considered as a bottleneck for making these machines adaptable and interoperable. Two new technologies emerged in recent years: Standard for the Exchange of Product data for Numerical Control (STEP-NC) and function blocks. The STEP-NC data model represents a common standard for NC programming, making the goal of a generic NC code generation facility a reality. Function blocks are an emerging IEC standard for distributed industrial processes and control systems. They can be used for CNC controls to encapsulate machining data, such as machining features and their needed algorithms. This paper introduces the above two new standards and the technologies that are developed based on the standards. The main body is devoted to analyze the standards from the functionality viewpoint. These functionalities include, bidirectional information flow in computer-aided design/computer-aided manufacturing, data sharing over the Internet, the use of feature-based machining concept, modularity and reusability, intelligent and autonomous CNC, and portability among resources. Some implementations are also presented to showcase how the standards are used to develop technologies for interoperable machining. Note to Practitioners-Modern computer numerically controlled (CNC) machine tools are limited in functions because their controllers rely on G-codes for communications. G-code is considered a "dumb" language as it only documents instructional and procedural data, leaving most of the design information behind. G-code programs are also hardware dependent, denying modern CNC machine tools desired interoperability and portability. In recent years, two new standards emerged, STEP-NC and function blocks. They may hold the key to empowering CNC machine tools with richer information which, in turn, gives CNC machine tools the ability to "think" intelligently and to be interoperable. This paper introduces these two standards, the technologies that have been developed based on the standards and some prototype systems using the standards and technologies. The intention is not to highlight any achieved research outcome. Instead, the focus is on informing the research and practical world about these new standards, analyzing them from the viewpoint of supporting interoperable CNC machine tools, and offering some futuristic views about these standards and technologies. While these standards are still in their infancy, research activities and prototype systems are already coming thick and fast. There seems to be a "healthy" mixture of participants working in the field. They range from the manufacturers of all systems related to the data interface (i.e., CAM systems, controls, and machine tools), to the users and academic institutions.
Hypolipidemic Effects of 2‐Furoic Acid in <i>Sprague‐Dawley</i> Rats (1993)

2-Furoic acid was shown to be effective in lowering both serum cholesterol and serum triglyceride levels significantly in rats with an elevation of HDL cholesterol level at 20 mg/kg/day orally. LDL receptor activity was reduced in hepatocytes, aorta foam cells, small intestinal epithelium cells and fibroblasts. HDL receptor activity was elevated in the rat hepatocytes and small intestinal cells. These activities were correlated with inhibition of acyl CoA cholesterol acyl transferase activity. Neutral cholesterol ester hydrolase activity was elevated in rat hepatocytes and human fibroblasts. Thus, 2-furoic acid appears to interfere directly with activity of intracellular enzymes rather than affecting high affinity-mediated lipoprotein membrane receptors. In vivo treatment with 2-furoic acid led to reduction in the liver and small intestine ATP dependent citrate lyase, acetyl CoA synthetase, acyl CoA cholesterol acyl transferase, sn-glycerol 3-phosphate acyl transferase, phosphatidylate phosphohydrolase and heparin induced lipoprotein lipase activities. 2-Furoic acid reduced biliary cholesterol levels but the agent increased bile salts which are lithogenic. Acute toxicity studies in mice suggest that the agent has some hepatic toxicity effects. The LD50 was relatively low at 250 mg/kg IP in mice.
A switched-mode multichannel neural stimulator with a minimum number of external components (2013)

This work proposes a system design for neural stimulators. It eliminates the need for a DC-DC converter which is normally used to increase the battery voltage. Instead it combines the DC-DC converter and stimulator output stage into a single system block. The number of external components needed for the system is reduced to a single inductor only. The system offers high power efficiency operation (theoretical power efficiency of 100% and a simulated efficiency of around 60% over the full output range) by employing switched-mode operation. Furthermore the output has a current source character, making charge balanced stimulation relatively easy to implement. Finally it is also possible to stimulate multiple channels independently with this system. An example design is presented that can stimulate two channels with a maximum output voltage of 10 V, while being powered from a 3.5V battery.
Single-axis motion control of wind turbine (2013)

In this paper a single-axis motion control of wind turbine is discussed and wind turbine controller FBs is designed so as to use IEC 61131 FBs library for the wind turbine controller programming, which makes wind turbine controller software to be simple, clear and more flexible. The I/O correspondence between IEC 61131 Single-Axis FBs and wind turbine controller is discussed first. In view of the Betz's law from aerodynamics, the question about rotor speed as input variable instead of wind speed has been confirmed in this paper. After a brief introduction of the turbine control systems and general control principle of wind turbine, the single-axis FBs of wind turbine are proposed. A simplified FB network is used to describe pitch and torque motion control system. The software implementation of the signal-axis FBs are illustrated finally.
Nitric Oxide Produced by Inducible Nitric Oxide Synthase Delays Gastric Emptying in Lipopolysaccharide-treated Rats (1997)

The present study suggests that NO, probably produced by iNOS, is one of the factors involved in the delay of gastric emptying in the LPS-treated rats and probably in those with sepsis.
Inactivation and partition of human T‐cell lymphotrophic virus, type III, during ethanol fractionation of plasma (1986)

Because of concern about the safety of immune globulins prepared for injection, we studied the effects of ethanol fractionation of human plasma on human lymphotropic virus, type III, (HTLV-III) by spiking the products of various fractionation steps with HTLV-III. Tests of inactivation and removal indicated that the ratio of residual live virus in plasma fractions/live virus in starting plasma was about 1 X 10(-15) for precipitate II from which immune globulin for injection is manufactured. The results are reassuring regarding the potential safety of immune globulin.
Periosteally derived osteoblast-like cells differentiate into chondrocytes in suspension culture in agarose (2000)

Pluripotent cells from the periosteal layer adjacent to cortical bone attain an osteoblast-like phenotype in culture when reaching confluence in monolayer. It is unknown whether such newly differentiated osteoblast-like cells preserve the chondrogenic potential characteristics for stem cells derived from the periosteum. Primary osteoprogenitor cells derived from bovine metacarpal periosteum were differentiated into alkaline phosphatase-positive osteoblast-like cells by an established monolayer culture protocol. After transfer into suspension culture in agarose gels, the cells differentiated into chondrocytes demonstrated by the production of collagen II, but not of collagen I, as well as alkaline phosphatase activity was abated. Contrarily, with continuation of monolayer culture, the cells maintained their osteoblast-like phenotype and secreted large amounts of collagen I and a minor quantity of collagen III and V. The alkaline phosphatase activity steadily increased during the entire culture period of 2 weeks. Thus, our culture techniques can serve as useful tools to study mechanisms of differentiation by modulating the phenotypic potential of osteogenic cells. The results presented here support the notion that the extracellular environment strongly influences the cell type and its metabolism.
Characterization of the Accommodation Effect in Soft Hysteretic Materials Via Sensorless Measurement Technique (2009)

The well-known voltamperometric method has only been used so far in the identification of symmetric hysteresis cycles for reasons that are explained in the main text. This paper extends the sensorless measurement method to the characterization of asymmetric loops, without losing the main advantage of this technique, i.e., the low-cost setup and the simplicity of the procedure. The current through-and the induced voltage on-the primary and secondary coils, which are wound around a magnetic core, are the measurands. Moreover, by carefully choosing the input signals, so that a series of major loops is defined along the whole <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">B</i> - <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">H</i> measured path, a way is suggested to avoid the problems caused by the dc offset of the instrumentation chain and to reduce the uncertainty in the determination of the initial state of magnetization. Both the offset and the initial magnetization state are necessary for a correct reconstruction of the induction field. A complete analysis of the capabilities and critical aspects of the proposed measurement procedure is carried out through a theoretical discussion supported by simulation and experimental results. The measurement of nonsymmetric cycles and their accommodation are shown and discussed as the final objective of the proposed approach.
David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody Fragments (2019)

Since the licensing of the first monoclonal antibody therapy in 1986, monoclonal antibodies have become the largest class of biopharmaceuticals with over 80 antibodies currently approved for a variety of disease indications. The development of smaller, antigen binding antibody fragments, derived from conventional antibodies or produced recombinantly, has been growing at a fast pace. Antibody fragments can be used on their own or linked to other molecules to generate numerous possibilities for bispecific, multi-specific, multimeric, or multifunctional molecules, and to achieve a variety of biological effects. They offer several advantages over full-length monoclonal antibodies, particularly a lower cost of goods, and because of their small size they can penetrate tissues, access challenging epitopes, and have potentially reduced immunogenicity. In this review, we will discuss the structure, production, and mechanism of action of EMA/FDA-approved fragments and of those in clinical and pre-clinical development. We will also discuss current topics of interest surrounding the potential use of antibody fragments for intracellular targeting and blood-brain barrier (BBB) penetration.
Fused Heterocycles, Furo[3,2-<i>d</i>]pyrimidines and Dihydrocyclopenta[d]pyrimidines, as Potential New Herbicides (1992)

(1992). Fused Heterocycles, Furo[3,2-d]pyrimidines and Dihydrocyclopenta[d]pyrimidines, as Potential New Herbicides. Bioscience, Biotechnology, and Biochemistry: Vol. 56, No. 11, pp. 1897-1899.
Piezoelectric sensorics: force, strain, pressure, acceleration and acoustic emission sensors, materials and amplifiers (2002)

1 Introduction.- 2 Background of Piezoelectric Sensors.- 3 Piezoelectric Materials for Sensors.- 4 Piezoelectric Sensor Terminology.- 5 Piezoelectric Sensors.- 6 Force and Torque Sensors.- 7 Strain Sensors.- 8 Pressure Sensors.- 9 Acceleration Sensors.- 10 Acoustic Emission Sensors.- 11 Amplifiers for Piezoelectric Sensors.- References.- List of Manufacturers.
Overlapping roles of two Hox genes and the<i>exd</i>ortholog<i>ceh-20</i>in diversification of the<i>C. elegans</i>postembryonic mesoderm (2000)

Members of the Hox family of homeoproteins and their cofactors play a central role in pattern formation of all germ layers. During postembryonic development of C. elegans, non-gonadal mesoderm arises from a single mesoblast cell M. Starting in the first larval stage, M divides to produce 14 striated muscles, 16 non-striated muscles, and two non-muscle cells (coelomocytes). We investigated the role of the C. elegans Hox cluster and of the exd ortholog ceh-20 in patterning of the postembryonic mesoderm. By examining the M lineage and its differentiation products in different Hox mutant combinations, we found an essential but overlapping role for two of the Hox cluster genes, lin-39 and mab-5, in diversification of the postembryonic mesoderm. This role of the two Hox gene products required the CEH-20 cofactor. One target of these two Hox genes is the C. elegans twist ortholog hlh-8. Using both in vitro and in vivo assays, we demonstrated that twist is a direct target of Hox activation. We present evidence from mutant phenotypes that twist is not the only target for Hox genes in the M lineage: in particular we show that lin-39 mab-5 double mutants exhibit a more severe M lineage defect than the hlh-8 null mutant.
Formation of banana phases in bent-shaped molecules with unusual bent angles as low as 60° (2009)

We prepared a homologous series of bent-shaped molecules with a low bent angle of about 60° using a 1,7-naphthalene central core, a long side wing in which one phenyl ring is attached to the classical wing via an ester linkage and alkoxy tail groups with numbers of carbons of 4 to 14. All the compounds form well-defined mesophases. Two calamitic nematic (N) and SmA phases, and two banana SmAPA and B4 phases were identified with a sequence of N–SmA–SmAPA–B4 upon decreasing temperature. In all these phases, molecules behave as bent molecules irrespective of the low bent angle of approximately 60°, and are packed with the bent direction perpendicular to the n-director. SmAPA is different from SmA only by having biaxiality, and exhibits antiferroelectric switching characteristic of banana smectic phases.
Small yet effective (2010)

The Ethylene-responsive element binding factor-associated Amphiphilic Repression (EAR) motif is a small yet distinct regulatory motif that is conserved in many plant transcriptional regulator (TR) proteins associated with diverse biological functions. We have previously established a list of high-confidence Arabidopsis EAR repressors, the EAR repressome, comprising 219 TRs belonging to 21 different TR families. This class of proteins and the sequence context of the EAR motif exhibited a high degree of conservation across evolutionarily diverse plant species. Our comprehensive genome-wide analysis enabled refining EAR motifs as comprising either LxLxL or DLNxxP. Comparing the representation of these sequence signatures in TRs to that of other repressor motifs we show that the EAR motif is the one most frequently represented, detected in 10 to 25% of the TRs from diverse plant species. The mechanisms involved in regulation of EAR motif function and the cellular fates of EAR repressors are currently not well understood. Our earlier analysis had implicated amino acid residues flanking the EAR motifs in regulation of their functionality. Here, we present additional evidence supporting possible regulation of EAR motif function by phosphorylation of integral or adjacent Ser and/or Thr residues. Additionally, we discuss potential novel roles of EAR motifs in plant-pathogen interaction and processes other than transcriptional repression.
Evaluation of range-separated hybrid density functionals for the prediction of vibrational frequencies, infrared intensities, and Raman activities (2008)

We present an assessment of different density functionals, with emphasis on range-separated hybrids, for the prediction of fundamental and harmonic vibrational frequencies, infrared intensities, and Raman activities. Additionally, we discuss the basis set convergence of vibrational properties of H2O with long-range corrected hybrids. Our results show that B3LYP is the best functional for predicting vibrational frequencies (both fundamental and harmonic); the screened-PBE hybrid (HSE) density functional works best for infrared intensities, and the long-range corrected PBE (LC-omegaPBE), M06-HF, and M06-L density functionals are almost as good as MP2 for predicting Raman activities. We show the predicted Raman spectrum of adenine as an example of a medium-size molecule where a DFT/Sadlej pVTZ calculation is affordable and compare our results against the experimental spectrum.
Development of Siliconized Epoxy Resins and Their Application as Anticorrosive Coatings (2011)

The present work involves the development of siliconized epoxy resin to overcome the drawback of epoxy resin like poor impact strength, high rigidity and moisture absorbing nature because of which they are not applied as corrosion resistant coating. By embedding silicone into the back bone of polymeric resin the above drawback can be reduced to substantial level. For achieving this, siliconised epoxy resins were prepared by reacting amine terminated silicone resin with novolac epoxy resin and meta-phenylenediamine was used as curing agent. The applied films of coating were baked at 150oC. Cured films were evaluated for their thermal, mechanical, chemical and corrosion resistance properties to ascertain the commercial utility of these eco-friendly resin for use in anti corrosive formulations. The siliconized epoxy resins system was found to exhibit good thermal and anticorrosive properties.
Design considerations and implementation of a cost-effective, portable remote monitoring unit using 3G wireless data networks (2005)

This paper describes the design and implementation of a light-weight, autonomous, patient-centric, portable medical unit that allows for anytime/anywhere monitoring and can find use in many monitoring scenarios like home-care, hospital wards, emergency help and disaster relief. The proposed unit called "TelePatient" achieves portability by exploiting a PDA and allows mobility through the use of cellular technology, enabling complete ubiquity. The design is based on open standards and is cost-effective. We incorporate transcoding software to fit data to limited bandwidth, as well as conserve energy on the power constrained PDA. We validate our design over real network 3G CDMA conditions and also test its use over WLAN802.11b, which can together cover a number of Telemedicine scenarios.
Invasion of erythrocytes by Plasmodium falciparum malaria parasites: evidence for receptor heterogeneity and two receptors (1986)

Plasmodium falciparum malaria parasites with different capabilities of invading sialic acid-deficient erythrocytes were identified. Thai-2 parasites cultured in Tn erythrocytes invaded neuraminidase-treated and Tn erythrocytes twice as efficiently as Thai-2 parasites cultured in normal erythrocytes and seven to ten times more efficiently than a cloned line of Camp parasites cultured in normal erythrocytes. All three parasite lines required sialic acid for optimal invasion, but Thai-2 parasites cultured in Tn erythrocytes invaded neuraminidase-treated erythrocytes with 45% efficiency whereas Camp parasites invaded neuraminidase-treated erythrocytes with less than 10% efficiency. P falciparum malaria parasites probably possess two receptors: one that binds to a sialic acid-dependent ligand and another that binds to a sialic acid-independent ligand. Parasites may differ in the quantity or affinity of their receptors for the sialic acid-independent ligand.