gebhart/patrepeval huggingface dataset:
Methionine aminopeptidases type I and type II are essential to control cell proliferation (2005)

The dependence of cell growth on methionine aminopeptidase (MetAP) function in bacteria and yeast is firmly established. Here we report experimental evidence that the control of cell proliferation in mammalian cells is directly linked and strictly dependent on the activity of both MetAP-1 and MetAP-2. The targeted downregulation of either methionine aminopeptidase MetAP-1 or MetAP-2 protein expression by small interfering RNA (siRNA) significantly inhibited the proliferation of human umbilical vein endothelial cells (HUVEC) (70%-80%), while A549 human lung carcinoma cell proliferation was less inhibited (20%-30%). The cellular levels of MetAP-2 enzyme were measured after MetAP-2 siRNA treatment and found to decrease over time from 4 to 96 h, while rapid and complete depletion of MetAP-2 enzyme activity was observed after 4 h treatment with two pharmacological inhibitors of MetAP-2, PPI-2458 and fumagillin. When HUVEC and A549 cells were treated simultaneously with MetAP-2 siRNA and PPI-2458, or fumagillin, which irreversibly inhibit MetAP-2 enzyme activity, no additive effect on maximum growth inhibition was observed. This strongly suggests that MetAP-2 is the single critical cellular enzyme affected by either MetAP-2 targeting approach. Most strikingly, despite their significantly different sensitivity to growth inhibition after targeting of either MetAP-1 or MetAP-2, HUVEC, and A549 cells, which were made functionally deficient in both MetAP-1 and MetAP-2 were completely or almost completely inhibited in their growth, respectively. This closely resembled the observed growth inhibition in genetically double-deficient map1map2 yeast strains. These results suggest that MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation and that MetAP-dependent growth control is evolutionarily highly conserved.
Seven new monogalactosyl diacylglycerols isolated from the axenic cyanobacterium Phormidium tenue. (1990)

Seven new galactolipids (2 to 8) were isolated from the cultured cyanobacterium P. tenue as substances causing lysis of this alga. The structures of these galactolipids were determined by physicochemical evidence and enzymatic hydrolysis using lipase. Those galactolipids which contained unsaturated fatty acids were more potently autolytic.
ChemInform Abstract: Hydrothermal Preparation and Characterization of Luminescent CdWO<sub>4</sub> Nanorods. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Sensor Based Motion Planning: The Hierarchical Generalized Voronoi Graph (1997)

Sensor based motion planning incorporates sensor information reflecting the state of a robot's environment into its planning process, whereas traditional approaches assume complete prior knowledge of the robot's environment. Recent research has focused on the development and incremental construction of the hierarchical generalized Voronoi graph (HGVG), which is a concise representation of a robot's environment. The HGVG is advantageous in that it lends itself to sensor based construction in a rigorous and provably correct manner. With this approach, a robot can enter an unknown environment, incrementally construct the HGVG, and then use the HGVG for future excursions in the environment. Simulations and experiments validate this approach.
Monocyte‐mediated entry of pathogens into the central nervous system (1990)

The origin of the microglia has long been a subject of debate. However it is now clear that monocytes enter the normal central nervous system and follow a series of morphological transformations as they differentiate into microglia. Thus, microglia are of monocytic origin. Since monocytes migrate into the normal CNS, they represent potential vehicles for the entry of pathogens into the nervous system and indeed may carry particulate matter into the CNS. Both viruses and bacteria use this 'Trojan horse' mechanism of entry in the pathogenesis of CNS disease.
Electron-hole recombination in disordered organic semiconductors: Validity of the Langevin formula (2009)

Accurate modeling of electron-hole recombination in organic light-emitting diodes (OLEDs) is essential for developing a complete description of their functioning. Traditionally, the recombination rate is described by the Langevin formula, with a proportionality factor equal to the sum of the electron and hole mobilities. In the disordered organic semiconductors used in OLEDs these mobilities have been shown to depend strongly on the carrier densities and on the electric field. Moreover, the energetic disorder leads to percolating pathways for the electron and hole currents, which may or may not be correlated. To answer the question whether the Langevin formula is still valid under such circumstances we perform Monte Carlo simulations of the recombination rate for Gaussian energetic disorder. We vary the disorder energy, the temperature, the densities, and mobility ratio of electrons and holes, the electric field, and the type of correlation between the electron and hole energies. We find that at zero electric field the Langevin formula is surprisingly well obeyed, provided that a change in the charge-carrier mobilities due to the presence of charge carriers of the opposite type is taken into account. Deviations from the Langevin formula at finite electric field are small at the field scale relevant for OLED modeling.
Selective Activation of Antigen-Experienced T Cells by Anti-CD3 Constrained on Nanoparticles (2013)

Activation of T cells through the TCR is mediated by the TCR-CD3 signaling complex. Cross linking of this complex with Abs directed against CD3 leads to potent activation of T cells. However, such activation is not Ag-specific. We exploited the observation that the TCR-CD3 complex is clustered on T cells that have been activated by Ag by using anti-CD3 nanoparticles to selectively activate Ag-experienced mouse T cells. We find that constraining anti-CD3 on the surface of a nanoparticle markedly and selectively enhances proliferation and cytokine production of Ag-experienced T cells but does not activate naive T cells. This effect was recapitulated in heterogeneous cultures containing mixtures of Ag-specific CD4(+) or CD8(+) T cells and bystander T cells. Furthermore, in vivo anti-CD3-coated nanoparticles increased the expansion of Ag-specific T cells following vaccination. Overall, these findings indicate that anti-CD3-coated nanoparticles could be use to enhance the efficacy of vaccines and immunotherapy. The results also suggest constraining a ligand on the surface of a nanoparticle might as general strategy for selectively targeting clustered receptors.
Autonomes Fahren auf der Autobahn – eine Potentialstudie für zukünftige Fahrerassistenzsysteme (2012)

Automatisiertes Fahren ohne Fahrereingriff auf der Autobahn, Vision oder bereits machbar? Im Rahmen der Technologie-Potentialstudie „hochautomatisiertes Fahren auf der Autobahn (HAF)“ der BMW Group Forschung und Technik wird ein Versuchsfahrzeug fur eine hochautomatisierte Autobahnfahrt entwickelt. Die Ergebnisse dieses Forschungsprojekts geben Auskunft uber die technische Machbarkeit und heutige Grenzen, vor allem aber wird dabei wertvolles Grundlagenwissen fur zukunftige Fahrerassistenzsysteme (FAS) gewonnen.
Sympathetic predominance an essential hypertension: a study employing spectral analysis of heart rate variability (1988)

In this study on 91 subjects we tested the hypothesis of an enhanced sympathetic activity in uncomplicated essential hypertension employing spectral analysis of heart rate variability. With this technique the tonic sympathetic and vagal activities and their changes are respectively assessed by the power of approximately 0.1 Hz (low frequency, LF) and approximately 0.25 Hz (respiratory linked, high frequency, HF) components of the spectrum of the beat by beat variability of RR interval. When comparing the 40 subjects with diastolic blood pressure consistently greater than 95 mmHg (hypertensives, Ht), with 35 age-matched controls (diastolic arterial pressure less than 90 mmHg, Nt), we observed that LF was greater and HF smaller in Ht as compared to Nt, thus suggesting an enhanced sympathetic activity and a reduce vagal activity in Ht. Additionally, passive tilt, which in Nt enhances LF [delta = 26 +/- 2 normalized units (nu)] and reduces HF (delta = -22 +/- 2, nu), produced smaller (P less than 0.05) changes in Ht (delta LF = 6.3 +/- 2.7 and delta HF = -7.5 +/- 2.3 nu). Furthermore, the values of LF at rest and the altered effects of tilt on LF and HF were significantly correlated with the degree of the hypertensive state. Chronic beta-adrenergic blockade (atenolol 100 mg once daily for 2 weeks, n = 13) reduced heart rate and blood pressure (from 162/103 to 136/88 mmHg) together with a significant diminution of LF and an increase of HF. Thus, spectral analysis of RR variability appears to be a convenient non-invasive technique to follow the progressive alterations in sympatho-vagal balance present in essential hypertension.
Advances in Gene Therapy for Diseases of the Eye (2016)

Over the last few years, huge progress has been made with regard to the understanding of molecular mechanisms underlying the pathogenesis of neurodegenerative diseases of the eye. Such knowledge has led to the development of gene therapy approaches to treat these devastating disorders. Challenges regarding the efficacy and efficiency of therapeutic gene delivery have driven the development of novel therapeutic approaches, which continue to evolve the field of ocular gene therapy. In this review article, we will discuss the evolution of preclinical and clinical strategies that have improved gene therapy in the eye, showing that treatment of vision loss has a bright future.
Experience of Retrofitting Automotive Air-Conditioning from CFC12 to HFC134a (1993)

<div class="htmlview paragraph">A method of retro-fitting automotive air-conditioning systems from CFC12 to HFC134a has been developed.</div> <div class="htmlview paragraph">This method only requires the use of conventional refrigerant recovery and recycle machines and offers a cost effective method of retrofitting a range of automotive air-conditioning systems to HFC 134a.</div>
Efficacy of Co-administration of Garlic Extract and Metformin for Prevention of Gentamicin-Renal Toxicity in Wistar Rats: A Biochemical Study. (2013)

The results indicate that MF and garlic or their combination have curative and protective activity against GM nephrotoxicity.
Robust Neighbor Discovery Through SideLink Demodulation Reference Signal for LTE ProSe Network (2019)

By introducing the Proximity Services (ProSe) functionalities in its 12 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sup> release, the 3 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">rd</sup> Generation Partnership Project (3GPP) made possible the ability to perform device-to-device (D2D) communication in Long Term Evolution-Advanced (LTE-A) cellular network. Direct communication between nearby User Equipments (UEs) is then enabled through the newly introduced sidelink. This paper investigates neighbor discovery for D2D communication exploiting Demodulation Reference Signals (DMRS), which are associated with physical sidelink channels for coherent demodulation. By simply listening to its sidelink signal, a ProSe-enabled UE can identify its peers and initiate a direct link without any additional overhead. The study examines the properties of DMRS sequences and proposes a robust neighbor discovery scheme tailored to DMRS structure based on a power normalized correlation process. A reduction in the computational load required to establish a link between two neighbors is proposed and evaluated. Simulation results show that nearby users can be discovered with a reasonable complexity while achieving a high discovery accuracy.
Fertility Therapy May Aid Gene Transfer (1999)

The premillennium frenzy about cloned drug-secreting sheep and cows, or pigs that have been given human genes in hopes of using them as organ donors, tends to gloss over the fact that introducing foreign genes into animals other than mice is still very difficult. Because current techniques—which primarily involve injecting DNA directly into fertilized eggs—have only a modest success rate, costs can soar to more than $300,000 for a single cow carrying a foreign gene. Now, genome tinkers may have a new tool for beefing up animal genomes with exotic DNA: sperm.
Eukaryotic Molybdopterin Synthase (1999)

We describe the primary structure of eukaryotic molybdopterin synthase small and large subunits and compare the sequences of the lower eukaryote, Aspergillus nidulans, and a higher eukaryote, Homo sapiens. Mutants in the A. nidulans cnxG (encoding small subunit) and cnxH (large subunit) genes have been analyzed at the biochemical and molecular level. Chlorate-sensitive mutants, all the result of amino acid substitutions, were shown to produce low levels of molybdopterin, and growth tests suggest that they have low levels of molybdoenzymes. In contrast, chlorate-resistant cnx strains have undetectable levels of molybdopterin, lack the ability to utilize nitrate or hypoxanthine as sole nitrogen sources, and are probably null mutations. Thus on the basis of chlorate toxicity, it is possible to distinguish between amino acid substitutions that permit a low level of molybdopterin production and those mutations that completely abolish molybdopterin synthesis, most likely reflecting molybdopterin synthase activity per se. Residues have been identified that are essential for function including the C-terminal Gly of the small subunit (CnxG), which is thought to be crucial for the sulfur transfer process during the formation of molybdopterin. Two independent alterations at residue Gly-148 in the large subunit, CnxH, result in temperature sensitivity suggesting that this residue resides in a region important for correct folding of the fungal protein. Many years ago it was proposed, from data showing that temperature-sensitive cnxH mutants had thermolabile nitrate reductase, that CnxH is an integral part of the molybdoenzyme nitrate reductase (MacDonald, D. W., and Cove, D. J. (1974) Eur. J. Biochem. 47, 107-110). Studies of temperature-sensitive cnxH mutants isolated in the course of this study do not support this hypothesis. Homologues of both molybdopterin synthase subunits are evident in diverse eukaryotic sources such as worm, rat, mouse, rice, and fruit fly as well as humans as discussed in this article. In contrast, molybdopterin synthase homologues are absent in the yeast Saccharomyces cerevisiae. Precursor Z and molybdopterin are undetectable in this organism nor do there appear to be homologues of molybdoenzymes.
Reference Resolution Challenges for an Intelligent Agent: The Need for Knowledge (2009)

This paper presents a vision of how language-endowed, next- generation intelligent agents might resolve – i.e., fully interpret – references to objects and events in language input. It describes some of the more difficult reference phenomena that are not being sufficiently treated by practical systems and suggests what kinds of knowledge must be available to intelligent agents to enable them to reach human competence in reference resolution.
Synthesis and activity of four (<i>N</i>,<i>N</i>‐dimethylamino)benzamide nonsteroidal anti‐inflammatory drugs based on thiazole and thiazoline (2006)

Abstract magnified image Four compounds derived from 2‐aminothiazole and 2‐amino‐2‐thiazoline were prepared by coupling the respective bases with the acid chlorides of either 3‐ or 4‐( N , N ‐dimethylamino)benzoic acid. Products were identified using infrared spectroscopy, 1 H NMR spectroscopy and electrospray mass spectroscopy and in two cases by single‐crystal X‐ray diffraction. Of the four, N ‐(thiazol‐2‐yl)‐3‐( N , N ‐dimethylamino)‐benzamide (1), N ‐(thiazolin‐2‐yl)‐4‐( N , N ‐dimethylamino)benzamide (2), N ‐(thiazolin‐2‐yl)‐3‐( N , N ‐dimethylamino) benzamide (3) and N ‐(thiazolin‐2‐yl)‐4‐( N , N ‐dimethylamino)benzamide (4), the hydrochloride salts of compounds 3 and 4 showed anti‐inflammatory activity across a concentration range of 10 −2 −5 × 10 −4 M while 3 (at a concentration of 10 −5 M ) was found to have no adverse effect on myocardial function. The X‐ray crystal structure of 2 and the 1:1 adduct structure of 3 with 3‐( N , N ‐dimethylamino)benzoic acid are reported.
A cAMP Biosensor-Based High-Throughput Screening Assay for Identification of Gs-Coupled GPCR Ligands and Phosphodiesterase Inhibitors (2015)

Cyclic adenosine 3',5'-monophosphate (cAMP) is an important second messenger, and quantification of intracellular cAMP levels is essential in studies of G protein-coupled receptors (GPCRs). The intracellular cAMP levels are regulated by the adenylate cyclase (AC) upon activation of either Gs- or Gi-coupled GPCRs, which leads to increased or decreased cAMP levels, respectively. Here we describe a real-time Förster resonance energy transfer (FRET)-based cAMP high-throughput screening (HTS) assay for identification and characterization of Gs-coupled GPCR ligands and phosphodiesterase (PDE) inhibitors in living cells. We used the β2-adrenergic receptor (β(2)AR) as a representative Gs-coupled receptor and characterized two cell lines with different expression levels. Low receptor expression allowed detection of desensitization kinetics and delineation of partial agonism, whereas high receptor expression resulted in prolonged signaling and enabled detection of weak partial agonists and/or ligands with low potency, which is highly advantageous in large HTS settings and hit identification. In addition, the assay enabled detection of β(2)AR inverse agonists and PDE inhibitors. High signal-to-noise ratios were also observed for the other representative Gs-coupled GPCRs tested, GLP-1R and GlucagonR. The FRET-based cAMP biosensor assay is robust, reproducible, and inexpensive with good Z factors and is highly applicable for HTS.
Introduction (1979)

Of the major European powers only Russia had an ‘intelligentsia question’. Elsewhere, wrote the literary critic and historian of the intelligentsia D. Ovsyaniko-Kulikovsky in 1908 there was discussion of science, politics, philosophy and art; in Russia there was only discussion of the nature of the intelligentsia.’ The purpose of this study is to examine the parameters of this discussion in one of its last and most intense phases, that which occurred at the time of the attempted revolution of 1905. In view of the unfamiliarity of, first, the concept of an ‘intelligentsia’ as it was understood in Russia and, second, the context of this last outburst of the debate, it is necessary to make a few preliminary remarks, though this in itself is difficult in that the answers to the preliminary questions about the intelligentsia are the stuff of the debate itself and to give a full answer would pre-empt all that follows.
Simultaneous Detection of BCL-2 Protein, Trisomy 12, Retinoblastoma and P53 Monoallelic Gene Deletions in B-Cell Chronic Lymphocytic Leukemia by Fluorescence in Situ Hybridization (FISH): Relation to Disease Status (2000)

Various genetic abnormalities are often found in B-CLL, but their relative importance in the pathogenesis and evolution of the disease has not been adequately clarified. We studied the expression of bcl-2 protein and the possible simultaneous occurrence of bcl-2 overexpression, trisomy 12 and the Rb1 and p53 gene deletions in 38 patients with B-CLL by combining immunophenotyping and dual color interphase FISH. We also looked for correlation between the genetic abnormalities and clinical parameters such as stage, disease duration from diagnosis to the time of study and overall survival. High expression of the bcl-2 protein was found in 76.3% of the patients (29/38). Trisomy 12 was found in 37% of cases (14/38) and Rb1 monoallelic gene deletion in 42% (16/38). The percentage of cells with hemizygous Rb1 deletion ranged from 13 to 18%. Monoallelic deletion of p53 was found in 29% of cases (11/38). The number of cells with only one signal ranged from 28 to 98%. Patients in stage A had on average, less than one abnormality, while patients in stage C had 2.6 abnormalities. Patients appeared to accumulate genetic abnormalities with time. Bcl-2 overexpression was found early in the course of the disease. Trisomy 12 appeared later, at about the same time as Rb1 deletion, but was not associated with adverse prognosis. Monoallelic deletion of p53 gene appeared rather late in the course of the disease and was associated with advanced stage. Despite the fact that more deaths occurred in the group of patients with three or four abnormalities and the presence of p53 gene deletion, differences in survival were not statistically significant, probably due to the limited number of patients in each group. A larger group of patients studied in a prospective manner will better clarify these issues in the future.
Implementation of baseband chip for the terrestrial digital multimedia broadcasting (T-DMB) system (2005)

A new national standard for terrestrial digital multimedia broadcasting (T-DMB) has been announced in Korea to provide high quality digital audio, video, and data broadcasting services to fixed, mobile, and portable receivers. We have developed an FPGA/ASIC implementation for the T-DMB system. The paper describes the features and functions of the newly developed T-DMB system. We also present the design, implementation, and performance of the baseband chip for the T-DMB system. The chip was fabricated using a 0.25 /spl mu/m 1P4M CMOS process.
Organometallic precursors in the growth of epitaxial thin films of III-V semiconductors by metal-organic chemical vapor deposition (MOCVD) (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganometallic precursors in the growth of epitaxial thin films of III-V semiconductors by metal-organic chemical vapor deposition (MOCVD)P. Zanella, G. Rossetto, N. Brianese, F. Ossola, M. Porchia, and J. O. WilliamsCite this: Chem. Mater. 1991, 3, 2, 225–242Publication Date (Print):March 1, 1991Publication History Published online1 May 2002Published inissue 1 March 1991https://pubs.acs.org/doi/10.1021/cm00014a008https://doi.org/10.1021/cm00014a008research-articleACS PublicationsRequest reuse permissionsArticle Views624Altmetric-Citations65LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Calibration and discrimination by daily Logistic Organ Dysfunction scoring comparatively with daily Sequential Organ Failure Assessment scoring for predicting hospital mortality in critically ill patients* (2002)

Daily LOD and SOFA scores showed good accuracy and internal consistency, and they could be used to adjust severity for events occurring in the intensive care unit.