gebhart/patrepeval huggingface dataset:
Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity. (1992)

Binding of GTP induces alpha subunits of heterotrimeric G proteins to take on an active conformation, capable of regulating effector molecules. We expressed epitope-tagged versions of the alpha subunit (alpha s) of Gs in genetically alpha s-deficient S49 cyc- cells. Addition of a hemagglutinin (HA) epitope did not alter the ability of wild type alpha s to mediate hormonal stimulation of adenylyl cyclase or to attach to cell membranes. The HA epitope did, however, allow a mAb to immunoprecipitate the recombinant protein (HA-alpha s) quantitatively from cell extracts. We activated the epitope-tagged alpha s in intact cells by: (a) exposure of cells to cholera toxin, which activates alpha s by covalent modification; (b) mutational replacement of arginine-201 in HA-alpha s by a cysteine residue, to create HA-alpha s-R201C; like the cholera toxin-catalyzed modification, this mutation activates alpha s by slowing its intrinsic GTPase activity; and (c) treatment of cells with the beta-adrenoceptor agonist, isoproterenol, which promotes binding of GTP to alpha s, thereby activating adenylyl cyclase. Both cholera toxin and the R201C mutation accelerated the rate of degradation of alpha s (0.03 h-1) by three- to fourfold and induced a partial shift of the protein from a membrane bound to a soluble compartment. At steady state, 80% of HA-alpha s- R201C was found in the soluble fraction, as compared to 10% of wild type HA-alpha s. Isoproterenol rapidly (in < 2 min) caused 20% of HA-alpha s to shift from the membrane-bound to the soluble compartment. Cholera toxin induced a 3.5-fold increase in the rate of degradation of a second mutant, HA-alpha s-G226A, but did not cause it to move into the soluble fraction; this observation shows that loss of membrane attachment is not responsible for the accelerated degradation of alpha s in response to activation. Taken together, these findings show that activation of alpha s induces a conformational change that loosens its attachment to membranes and increases its degradation rate.
Leukocytes Classification and Segmentation in Microscopic Blood Smear: A Resource-Aware Healthcare Service in Smart Cities (2016)

Smart cities are a future reality for municipalities around the world. Healthcare services play a vital role in the transformation of traditional cities into smart cities. In this paper, we present a ubiquitous and quality computer-aided blood analysis service for the detection and counting of white blood cells (WBCs) in blood samples. WBCs also called leukocytes or leucocytes are the cells of the immune system that are involved in protecting the body against both infectious disease and foreign invaders. Analysis of leukocytes provides valuable information to medical specialists, helping them in diagnosing different important hematic diseases, such as AIDS and blood cancer (Leukaemia). However, this task is prone to errors and can be time-consuming. A mobile-cloud-assisted detection and classification of leukocytes from blood smear images can enhance accuracy and speed up the detection of WBCs. In this paper, we propose a smartphone-based cloud-assisted resource aware framework for localization of WBCs within microscopic blood smear images using a trained multi-class ensemble classification mechanism in the cloud. In the proposed framework, nucleus is first segmented, followed by extraction of texture, statistical, and wavelet features. Finally, the detected WBCs are categorized into five classes: basophil, eosinophil, neutrophil, lymphocyte, and monocyte. Experimental results on numerous benchmark databases validate the effectiveness and efficiency of the proposed system in comparison to the other state-of-the-art schemes.
Lithography-based ceramic manufacture (LCM) of auxetic structures: present capabilities and challenges (2016)

Auxetic metamaterials are known for having a negative Poisson's ratio (NPR) and for displaying the unexpected properties of lateral expansion when stretched and densification when compressed. Even though a wide set of micro-manufacturing resources have been used for the development of auxetic metamaterials and related devices, additional precision and an extension to other families of materials is needed for their industrial expansion. In addition, their manufacture using ceramic materials is still challenging. In this study we present a very promising approach for the development of auxetic metamaterials and devices based on the use of lithography-based ceramic manufacturing. The process stands out for its precision and complex three-dimensional geometries attainable, without the need of supporting structures, and for enabling the manufacture of ceramic auxetics with their geometry controlled from the design stage with micrometric precision. To our knowledge it represents the first example of application of this technology to the manufacture of auxetic geometries using ceramic materials. We have used a special three-dimensional auxetic design whose remarkable NPR has been previously highlighted.
Species Richness of Plant Parasites and Growth Form of Their Hosts (1979)

Trees tend to have more pest species than do shrubs, which in turn have more than herbs, per unit area of geographical distribution. These patterns are shown by species/area analyses of the insect associates of 215 genera of British plants and the fungal parasites of 323 United States plant species. Within these growth-form categories some comparisons show no discernible differences in pest species richness: Angiosperm trees have approximately the same number of fungal parasites as gymnosperms, and monocot herbs have about the same number of fungal parasites as do herbs in general, in the United States. British sedges, grasses, all monocots, composites, and bog plants do not support discernably different numbers of insect species than herbs in general, per unit host range. However, other comparisons within the herb-shrub-tree categories do show differences: Ferns support fewer insect species than other herb genera in Britain, and weedy herbs support fewer fungal parasite species than climax and subclimax herbs in the United States, per unit range of host species. We propose that the greater size and morphological complexity of trees than shrubs, and than herbs in turn, accounts for these patterns in pest species richness; larger more complex hosts present a greater diversity of pest niches. If one makes the assumption that these plant categories represent a spectrum of resource constancy to parasites, then our results might be interpreted to support the idea that parasite pressure will be slightly greater upon more "apparent" host species. Much residual variation remains about the species/area lines even after the effects of plant growth form have been subtracted. The residual variation might be explained by underreporting of pests, by differences in commonness of plant taxa of equal geographic range, and by variation in the effectiveness of plant morphology and plant chemistry in protecting the plant from pests.
A new look on the parallel implementation of the Shur algorithm for the solution of Toeplitz equations (2005)

The Shür algorithm is considered in this paper and two different computational organizations are studied. The first is equivalent to the well known "parallel" solution of the Toeplitz equations, the second gives an order recursive algorithm. A simple demonstration of the Shür recursion is outlined and a "superlattice" structure is given for the imple - mentation of both types of algorithms.
An Efficient Method for Simulating Frequency-Dependent Friction in Transient Liquid Flow (1975)

An efficient procedure is developed for simulating frequency-dependent friction in transient laminar liquid flow by the method of characteristics. The procedure consists of determining an approximate expression for frequency-dependent friction such that the use of this expression requires much less computer storage or computation time than the use of the exact expression. The derived expression for frequency-dependent friction approximates the exact expression very well in both time and frequency domains. Calculated results for a test system are compared with the experimental results so show that the approximate expression predicts accurately the surge pressures, pressure wave distortion as well as pressure attenuation in a liquid line.
Foreward Vol.3 (1970)

DOI: http://dx.doi.org/10.3329/mist.v3i0.8044
Long-term tests of capacitive humidity sensors (1994)

Capacitive air humidity sensors have emerged in increasing numbers, makes and qualities. Of all hygrometers available at present, it is estimated that 70-75% employ a capacitive type of sensor. In this paper the results of outdoor experiments over a period of more than one year are summarized for most of the commercially available types.
Experimental investigation of infrared rapid surface heating for injection molding (2006)

Abstract A low cost and practical infrared rapid surface heating system for injection molding is designed and investigated. The system was designed to assemble on the mold and a control system was used to operate the motion of the lamp holder. Four infrared halogen lamps (1 kW each) were used as the radiative source to heat the surface of mold insert. The temperature increase is verified on the mold plate with a thermal video system. Two types of specular reflectors combined with different bulb configurations were applied to study the heating ability of radiation heating. A modified spiral flow mold was used to test the enhancing filling ability of the rapid surface heating system. Three resins, PP, PMMA and PC were molded in the spiral flow injection molding experiments. If spherical reflector and centralized lamp configuration are used, the temperature at the center of the mold surface is the highest. The temperature of mold center surface is raised from 83°C to 188°C with 15 s of infrared heating. Because the surface temperature of the mold insert is higher than the glass transition temperature of resins before filling, the flow distance of resins in the modified spiral flow mold will be increased. The location effect of the infrared surface heating system on a thin‐long cavity was studied to demonstrate the possibility of using smaller infrared heating area on a large mold surface. A microprobe cavity also demonstrated that with the assistance of infrared heating technology the formability of a microprobe can be greatly improved. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 3704–3713, 2006
Adaptive object-model builder (2009)

An Adaptive Object-Model system represents user-defined classes, attributes, relationships, and behavior as metadata. This paper presents the Adaptive Object-Model Builder pattern that is used to construct AOM entities. An AOM Builder reads an externally stored build description to construct a build process. This process is then executed to construct a properly initialized AOM entity. Since an AOM Builder is driven by metadata descriptions of entities and their build processes, a single generic AOM Builder implementation can construct different entity types.
Optimization of the optical switching characteristics of two-section Fabry-Perot lasers (1993)

Bistable two-section Fabry-Perot lasers with a saturable absorber have been fabricated, comprehensively characterized, and optimized with respect to gain and optical switching contrast. Best device performance requires the individual optimization of both section lengths, and a proper choice of current conditions. Light has to be injected into the gain section, instead of the absorber section, to take full advantage of device qualities. Based on this optimization high, all optical switching gain (26 dB) and large contrast (18 dB) have been achieved simultaneously.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Duckweeds (Lemnaceae family): a potential source of protein and amino acids (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDuckweeds (Lemnaceae family): a potential source of protein and amino acidsLouis L. Rusoff, Ernest W. Blakeney Jr., and Dudley D. Culley Jr.Cite this: J. Agric. Food Chem. 1980, 28, 4, 848–850Publication Date (Print):July 1, 1980Publication History Published online1 May 2002Published inissue 1 July 1980https://pubs.acs.org/doi/10.1021/jf60230a040https://doi.org/10.1021/jf60230a040research-articleACS PublicationsRequest reuse permissionsArticle Views1431Altmetric-Citations97LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The protocol for the Cannabidiol in children with refractory epileptic encephalopathy (CARE-E) study: a phase 1 dosage escalation study (2018)

http://clinicaltrials.gov [Internet]. Bethesda (MD): National Library of Medicine (US). 2016 Dec 16. Identifier NCT03024827, Cannabidiol in Children with Refractory Epileptic Encephalopathy: CARE-E; 2017 Jan 19 [cited 2017 Oct]; Available from: http://clinicaltrials.gov/ct2/show/NCT03024827.
The CDS1 Gene Encoding CDP-diacylglycerol Synthase In Saccharomyces cerevisiae Is Essential for Cell Growth (1996)

An open reading frame (CDS1) residing on chromosome II of Saccharomyces cerevisiae encodes a hydrophobic protein with a predicted molecular mass of 51,789 Da, which exhibits 29 and 37% amino acid sequence identities with CDP-diacylglycerol synthases reported from Escherichia coli and Drosophila, respectively. Induction of expression of a GAL1 promoter-driven CDS1 gene on a multicopy plasmid in a cds1 null mutant background resulted in synthase activity 10 times that of wild-type cells and an elevation in the apparent initial rate of synthesis of phosphatidylinositol relative to phosphatidylserine. Without induction, activity was reduced to 10% of wild-type levels, which was sufficient to support growth but resulted in an inositol excretion phenotype, and had an opposite effect on the above phospholipid synthesis. Null cds1 mutants were incapable of spore germination or vegetative growth and could not be complemented under uninduced conditions with a GAL1 promoter-driven CDS1 gene on a low copy plasmid. Therefore, the essential CDS1 gene encodes the majority, if not all, of the synthase activity. The lack of consensus RNA splice sites derived from the genomic CDS1 sequence predicts that the multiple subcellular locations for synthase activities do not arise through RNA processing events.
Receptor-mediated transcytosis of IgA in MDCK cells is via apical recycling endosomes. (1994)

Classically, the polymeric immunoglobulin receptor and its ligand, IgA, are thought to be sorted from basolateral early endosomes into transcytotic vesicles that directly fuse with the apical plasma membrane. In contrast, we have found that in MDCK cells IgA is delivered from basolateral endosomes to apical endosomes and only then to the apical cell surface. When internalized from the basolateral surface of MDCK cells IgA is found to accumulate under the apical plasma membrane in a compartment that is accessible to two apically added membrane markers: anti-secretory component Fab fragments, and avidin internalized from the biotinylated apical pole of the cell. This accumulation occurs in the presence of apical trypsin, which prevents internalization of the ligand from the apical cell surface. Using a modification of the diaminobenzidine density-shift assay, we estimate that approximately 80% of basolaterally internalized IgA resides in the apical endosomal compartment. In addition, approximately 50% of basolaterally internalized transferrin, a basolateral recycling protein, has access to this apical endosomal compartment and is efficiently recycled back to the basolateral surface. Microtubules are required for the organization of the apical endosomal compartment and it is dispersed in nocodazole-treated cells. Moreover, this compartment is largely inaccessible to fluid-phase markers added to either pole of the cell, and therefore seems analogous to the recycling endosome described in nonpolarized cells. We propose a model in which transcytosis is not a specialized pathway that uses unique transcytotic vesicles, but rather combines portions of pathways used by non-transcytosing molecules.
Catharanthus Alkaloids. XXXV. Isolation of Leurosidine N'<sub>b</sub>-Oxide From Catharanthus roseus (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatharanthus Alkaloids. XXXV. Isolation of Leurosidine N'b-Oxide From Catharanthus roseusSibabrata Mukhopadhyay and Geoffrey A. CordellCite this: J. Nat. Prod. 1981, 44, 5, 611–613Publication Date (Print):September 1, 1981Publication History Published online1 July 2004Published inissue 1 September 1981https://pubs.acs.org/doi/10.1021/np50017a021https://doi.org/10.1021/np50017a021research-articleACS PublicationsRequest reuse permissionsArticle Views115Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Emerging Race to Cure HIV Infections (2011)

Timothy Ray Brown's startling fate has pushed to the front a daunting research challenge that long seemed a fool's errand
FOUR-ELEMENT DUAL-BAND MIMO ANTENNA SYSTEM FOR MOBILE PHONES (2015)

A dual-band multiple-input-multiple-output (MIMO) antenna system for LTE 700/2300/2500, UMTS2100, GSM 1800/1900 mobile phone applications is presented. The whole system consists of four identical 3-D IFAs (inverted F antenna) loaded with lumped inductors and folded on FR4 cuboids. Without any special designed decoupling structures, the measured isolation among antenna elements is higher than 13 dB. Return loss characteristics, correlation coefficient, gain and radiation performance are also presented.
Energy storage applications of biomass-derived carbon materials: batteries and supercapacitors (2017)

Recent advances in the application of biomass-derived carbon materials in batteries and supercapacitors.
Expression of the Reverse Tetracycline-Transactivator Gene Causes Emphysema-Like Changes in Mice (2006)

The doxycycline-inducible, gene regulatory system allows tight control of transgene expression for the study of organ development and disease pathogenesis. Multiple recent reports have employed this model to investigate various lung diseases including emphysema. For our study, we used this transgenic system to test whether prolonged, lung-specific, overexpression of the serine protease urokinase plasminogen activator (uPA) would result in alveolar wall destruction. Double transgenic mice were generated that possessed: (1) the rat Clara cell secretory protein promoter controlling the reverse tetracycline transactivator gene (CCSP:rtTA) and (2) the tetracycline operator controlling the murine uPA cDNA (tet[O]:muPA). Mice were treated with doxycycline beginning at age 6 wk to initiate uPA overexpression. Single transgenic and wild-type animals served as controls. A second group of double transgenic and control animals were maintained off of doxycycline. At ages 10, 18, and 30 wk, the mice underwent measurements of alveolar size, lung compliance, and total lung capacity. We found that, although the uPA overexpressing mice demonstrated an emphysema phenotype, similar abnormalities occurred in the CCSP-rtTA control animals. These CCSP-rtTA-related alterations occurred even without doxycycline exposure. Evaluation of a second transgenic line possessing the human surfactant protein C promoter controlling rtTA expression also exhibited lung abnormalities consistent with emphysema. These findings indicate that pulmonary epithelial expression of rtTA alone causes an emphysema phenotype in mice. Therefore, when using this system to study emphysema pathogenesis, the inclusion of proper controls is essential for accurate data interpretation.
A framework for protecting Web services with IPsec (2004)

This work proposes a WSDL security extension for describing IPsec policies in Web services negotiations. The proposed extension is based on the policy model proposed by IETF for describing IPsec policies. Besides the model, the paper presents a framework for embedding IPsec negotiations in Web services applications. The paper also presents the results of the overhead evaluation introduced by the use IPsec to protect Web services applications under different configurations.
Observation of ultrafast lattice heating using time resolved electron diffraction (2009)

We use ultrafast electron diffraction to study lattice heating of 20nm noble metal films after femtosecond optical excitation with moderate excitation fluences. Using the Debye–Waller effect, the rise times of the lattice temperature were measured to be 1.1ps in copper (5.9mJ∕cm2 incident fluence) and 4.7ps in gold (0.9mJ∕cm2).
Induction of programmed cell death (apoptosis) in mature lymphocytes (1991)

The apoptosis of human peripheral blood lymphocytes was analyzed by the breakdown of DNA into oligonucleosome-sized fragments. The mature lymphocytes were rendered sensitive to apoptosis by either the omission of fetal bovine serum in the culture medium or the addition of polymyxin B. In the first case it was counteracted by phorbol myristate acetate. The possible involvement of protein kinase C in cell survival is pointed out.