gebhart/patrepeval huggingface dataset:
Persistent intracellular calcium pool depletion by thapsigargin and its influence on cell growth. (1991)

The intracellular Ca2+ pump inhibitor, thapsigargin, added to DDT1MF-2 smooth muscle cells in culture, irreversibly inhibited accumulation of Ca2+ within cells, permanently emptied the inositol 1,4,5-trisphosphate (InsP3)-sensitive Ca2+ pool, and simultaneously induced profound alteration of cell growth. After only a brief (30-min) treatment of cultured cells with 3 microM thapsigargin followed by extensive washing, the total releasable InsP3-sensitive Ca2+ pool remained entirely empty, even after 7 days of culture without thapsigargin. After thapsigargin treatment, cells retained viability, usual morphology, and normal mitochondrial function. Despite the otherwise normal appearance and function of thapsigargin-treated cells, cell division was completely blocked by thapsigargin. DNA synthesis was completely inhibited when thapsigargin was added immediately after passaging, but was suppressed only slowly (4-6 h) when added to rapidly synthesizing cells (24 h after passaging). Protein synthesis was reduced by approximately 70% in thapsigargin-treated cells. The sensitivity of thapsigargin-mediated inhibition of cell division, DNA synthesis, protein synthesis, and Ca(2+)-pumping activity were all similar with the EC50 values for thapsigargin in each case being close to 10 nM. Upon application to DDT1MF-2 cells, thapsigargin transiently increased resting cytosolic Ca2+ (0.15 microM) to a peak of 0.3 microM within 50 s; thereafter, free Ca2+ declined to 0.2 microM by 150 s and continued to slowly decline toward resting levels. Cells treated with thapsigargin for 1-72 h in culture displayed normal resting cytosolic Ca2+ levels. However, application of thapsigargin or epinephrine to such cells resulted in no change in the intracellular Ca2+, indicating that the internal Ca2+ pool remained completely empty. These results suggest that emptying of Ca2+ from intracellular thapsigargin-sensitive Ca(2+)-pumping pools induces profound alteration of cell proliferation.
Transformation toughening of metastable austenitic steels (1984)

Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 1984.
Hormone-Dependent Regulation of Chicken Progesterone Receptor Deoxyribonucleic Acid Binding and Phosphorylation* (1989)

To further understand the structure-function relationships of the chicken oviduct progesterone receptor, the effects of in vivo and in situ progesterone treatment were studied. Immunoprecipitated receptors isolated from oviduct slices incubated in the presence of H(3)32PO4 exhibited hormone-dependent phosphorylation. This was correlated with an increase in the apparent mol wt of receptors when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and increased DNA binding of total cytosolic receptors. Further, in vivo progesterone treatment resulted in dissociation of both the A and B receptor forms from nonhormone-binding proteins (such as heat shock protein-90) in vitro when analyzed by sucrose gradient ultracentrifugation. The 4S and 8S receptors were separated by phosphocellulose column chromatography, treated with ammonium sulfate to convert all receptors to DNA-binding forms, and analyzed for binding to DNA cellulose. The 4S receptor produced as a consequence of in vivo hormone treatment had a 3.35-fold higher affinity for DNA and bound to about a 3-fold greater extent than receptor that did not show altered interaction with other proteins. Thus, in vivo progesterone treatment results in increased receptor phosphorylation, altered interaction with heat shock protein-90, and increased DNA binding.
Statistical analysis and autoregressive modeling of the indoor radio propagation channel (2003)

The results from a statistical analysis of indoor radio propagation data that were collected from two modern office buildings are presented. The data base for this analysis consists of 12000 measurements (6000 in each building) taken at specific locations throughout the buildings. The data consists of the measured complex transfer function of the indoor channel. The complex impulse response is obtained from an inverse Fourier transform. Values for the root mean square delay spread ( tau /sub rms/), coherence bandwidth (B/sub C/), and path loss are given. The results indicate that tau /sub rms/ is typically between 18 and 35 nanoseconds, that B/sub C/ is typically between 4 and 11 MHz, and that B/sub C/ and tau /sub rms/ are linearly related. Also, the results indicate that B/sub C/ (and hence, tau /sub rms/) is a strong function of path loss. The authors also present results from autoregressive modeling and show how they correspond to traditional channel characterization techniques like tau /sub rms/.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Low-Overhead Asynchronous Interconnection Network for GALS Chip Multiprocessors (2011)

A new asynchronous interconnection network is introduced for globally-asynchronous locally-synchronous (GALS) chip multiprocessors. The network eliminates the need for global clock distribution, and can interface multiple synchronous timing domains operating at unrelated clock rates. In particular, two new highly-concurrent asynchronous components are introduced which provide simple routing and arbitration/merge functions. Post-layout simulations in identical commercial 90 nm technology indicate that comparable recent synchronous router nodes have 5.6-10.7 more energy per packet and 2.8-6.4 greater area than the new asynchronous nodes. Under random traffic, the network provides significantly lower latency and identical throughput over the entire operating range of the 800 MHz network and through mid-range traffic rates for the 1.36 GHz network, but with degradation at higher traffic rates. Preliminary evaluations are also presented for a mixed-timing (GALS) network in a shared-memory parallel architecture, running both random traffic and parallel benchmark kernels, as well as directions for further improvement.
[Cytokine production by MTSC 4 cells, an established mouse thymic dendritic cell line]. (1994)

The cytokine production by MTSC 4 cells, a murine thymic dendritic cell line established in our laboratory, has been investigated. Without exogenous stimulation, the MTSC 4 cells constitutively produce multiple types of cytokines, including IL-1, IL-6, IL-7, CSF, IFN and Chemotactic factor (CF). Of which, IL-6 and IFN were abundant: IL-1 and Chemokine, moderate: IL-7 and CSF at low level. MTSC4 cells could not produce detectable either IL-3 of TNF alpha. The establishment of MTSC 4 may be useful in the analysis of the mechanism of thymus selection, and in the study of the network regulation of autocrined cytokines.
Silver(I) chemistry in aqueous alkaline media. 2. Study of the interaction of silver(I) with .beta.-disulfone carbanions in aqueous alkaline media (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSilver(I) chemistry in aqueous alkaline media. 2. Study of the interaction of silver(I) with .beta.-disulfone carbanions in aqueous alkaline mediaJohn R. DeMember, Hilton F. Evans, Fredrick A. Wallace, and Patricia A. TariverdianCite this: J. Am. Chem. Soc. 1983, 105, 17, 5647–5652Publication Date (Print):August 1, 1983Publication History Published online1 May 2002Published inissue 1 August 1983https://pubs.acs.org/doi/10.1021/ja00355a021https://doi.org/10.1021/ja00355a021research-articleACS PublicationsRequest reuse permissionsArticle Views90Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Methoxsalen in the Treatment of Vitiligo (1959)

Introduction and Purpose It has been shown that methoxsalen will enhance the ability of vitiliginous skin to repigment with prolonged exposure to sunlight, the effectiveness varying with the area of skin involved and with the intensity of treatment.<sup>1-12</sup>Assuming that 100% repigmentation will be attained if treatment is continued long enough and with sufficient intensity, then the question arises as to the permanency of this repigmentation. Must treatment be continued indefinitely, or can it be stopped when the degree of repigmentation is satisfactory and the skin still retain the new pigment? Previous reports have not been concerned with this aspect of the treatment of vitiligo with methoxsalen. The purpose of this study was to answer this practical question within the limits imposed by the period of time methoxsalen has been available for clinical trial and the availability of records and of patients for follow
A constrained adaptive beamformer for medical ultrasound: initial results (2003)

Adaptive beamforming has been widely used as a way to improve image quality in medical ultrasound applications by correcting phase and amplitude aberration errors resulting from tissue inhomogeneity. A less-studied concern in ultrasound beamforming is the deleterious contribution of bright off-axis targets. This paper describes a new approach, the constrained adaptive beamformer (CAB), which builds on classic array processing methods from radar and sonar. Given a desired frequency response for the mainlobe beam, the CAB reduces off-axis signals by imposing an optimal set of weights on the receive aperture. A linearly constrained adaptive filter dynamically adjusts the aperture weights in response to the incoming data. Initial results show a factor of two improvement in point target resolution and a 60% contrast improvement for low echogenicity cysts. The CAB could considerably improve cardiac and abdominal image quality. We address implementation issues and discuss future work.
Alterations in lymphopoiesis after hematopoietic reconstitution with IL-7 virus-infected bone marrow. (1993)

Murine bone marrow was infected with a helper-free recombinant retrovirus expressing the mIL-7 gene and used to reconstitute lethally irradiated hosts. Twenty-three percent of mIL-7 retrovirus-infected recipients became moribund within 4-16 wk posttransplant with splenomegaly and hyperplastic lymph nodes, elevated white blood cell counts, plus other noticeable abnormalities including, in one animal, lymphocytic ascites. FACS analysis of hematopoietic tissue in diseased mice revealed marked alterations in T cell subsets of spleen and lymph nodes. These differences in extrathymic tissues compared with control animals included increases in CD4(-)-CD8+ lymphocytes and most strikingly the appearance of large numbers of an unusual CD4(+)-CD8+ T cell population with other characteristics of immature thymocytes (CD3lo-Thy1(+)-HSAhi). 3H-thymidine incorporation assays performed on extrathymic lymphocytes from a lymph node or ascites of two affected mice showed high levels of proliferation in the absence of either CD3 cross linking or exogenous IL-7 stimulation. Interestingly, in contrast to the effects noted on peripheral lymphoid tissues, no alteration in thymic size was noted and the proportion of CD4(+)-CD8+ cells was generally decreased with corresponding increases in CD4+ or CD8+ or CD4(-)-CD8- cells. These results provide further evidence of the involvement of IL-7 in the development and proliferation of early T cells in vivo and point to the possibility of IL-7 involvement in extrathymic expansion of a primitive class of T cells, the functional nature of which remains to be elucidated.
Cancer screening in the United States, 2009: A review of current American Cancer Society guidelines and issues in cancer screening (2009)

Each year, the American Cancer Society (ACS) publishes a report summarizing its recommendations for early cancer detection, data and trends in cancer screening rates, and select issues related to cancer screening. In 2008, the ACS, the American Gastroenterological Association, the American College of Gastroenterology, the Society for Gastrointestinal Endoscopy, and the American College of Radiology issued a joint update of guidelines for colorectal cancer screening in average-risk adults. In this issue, the current ACS guidelines and recent issues are summarized, updates of testing guidelines for early prostate cancer detection and colorectal cancer screening by the United States Preventive Services Task Force are discussed, and the most recent data from the Centers for Disease Control and Prevention's Behavioral Risk Factor Surveillance System and the National Health Interview Survey pertaining to participation rates in cancer screening are described.
New insights into posttranslational modifications of Hippo pathway in carcinogenesis and therapeutics (2016)

PTMs (posttranslational modifications) such as ubiquitylation, sumoylation, acetylation and protein methylation are pivotal modifiers that determine the activation, deactivation or subcellular localization of signaling proteins, facilitating the initiation, amplification and transduction of signaling. Accumulating evidence suggest that several key signaling molecules in Hippo signaling pathway are tightly regulated by various types of PTMs. Malfunction of these critical signaling modules such as YAP/TAZ, MAT1/2 and LATS1/2 due to deregulated PTMs has been linked to a variety of human diseases such as cancer. In this review article, we summarized the current understanding of the impact of PTMs in regulating Hippo signaling pathway and further discussed the potential therapeutic intervention from the view of PTMs and Hippo pathway.
Assessment of apoptosis occurring in spleen cells from nitrogen mustard-treated or gamma-irradiated mice. (1998)

The short-term consequences on spleen cells of the intraperitoneal administration of nitrogen mustard (HN-2) to mice or of a whole-body gamma irradiation have been evaluated. Experiments were designed to assess the induction of apoptosis in spleen cells following exposure to these agents. The occurrence of this type of cell death was analysed by several methods, in particular the quantification in the blood of phosphotidylserine-bearing microparticles shed by apoptotic cells. In response to HN-2 or radiations, spleens undergo a rapid involution of their weight and cellularity. Ex vivo apoptosis occurs within 24 hours in cultured lymphocytes in a dose-dependent manner after both treatments. As compared with untreated controls, circulating microparticles increased 3-fold after the injection of 5 mg/kg of HN-2.
Dynamics of molecular transport by surfactants in emulsions (2012)

We consider the dynamics of equilibration of the chemical potential of a fluorophore in a monodisperse emulsion containing droplets with two initially different concentrations of the fluorophore. Although the exchange mechanism involves a single timescale at the droplet (microscopic) level, the organisation of the droplets determines the exchange dynamics at the population (macroscopic) level. The micelle concentration in the continuous phase and the chemistry of the fluorophore control the microscopic exchange rate while the disorder of the initial condition determines the power-law of the long timescale, recovered in a minimal analytical model. We also show here that an additive in the droplet such as Bovine Serum Albumin (BSA) acts on the microscopic exchange rate and slows down the exchange process by increasing the solubility of the fluorophore in the dispersed phase rather than by creating a viscoelastic layer at the droplet interface.
Remote and cross-stack measurement of stack gas concentrations using a mobile FT-IR system (1982)

The EPA remote optical sensing of emissions (ROSE) system consists of a commercial FT-IR system and associated components installed in a van. The ROSE system was recently used to make both single-ended measurements of the emission spectra of the gas plume exiting a cement plant smokestack and also cross-stack measurements of the absorption spectra of the stack gases. The stack gases were also analyzed by conventional extractive techniques. The species NO, CO, CO(2), and NH(3) were observed in emission and absorption at concentrations of the order of 400, 50, 120,000, and 10 ppm, respectively. The species HC1, H(2)CO, HF, and SO(2) (typical concentrations of 20, 6, 0.5, and 40 ppm, respectively) were observed in absorption. Stack plume temperatures determined from the emission spectra agreed with in-stack temperature measurements to within +/-10%; concentration measurements agreed to within about +/-20%. This paper discusses the measurement and calibration procedures and shows the spectral signatures for the various species observed in the emission and absorption measurements.
Selective Benzopyranone and Pyrimido[2,1-<i>a</i>]isoquinolin-4-one Inhibitors of DNA-Dependent Protein Kinase:  Synthesis, Structure−Activity Studies, and Radiosensitization of a Human Tumor Cell Line in Vitro (2004)

A diverse range of chromen-2-one, chromen-4-one and pyrimidoisoquinolin-4-one derivatives was synthesized and evaluated for inhibitory activity against the DNA repair enzyme DNA-dependent protein kinase (DNA-PK), with a view to elucidating structure-activity relationships for potency and kinase selectivity. DNA-PK inhibitory activity varied widely over the series of compounds evaluated (IC(50) values ranged from 0.19 to >10 microM), with excellent activity being observed for the 7,8-benzochromen-4-one and pyrimido[2,1-a]isoquinolin-4-one templates. By contrast, inhibitors based on the benzochromen-2-one (coumarin) or 2-aryl-7,8-benzochromen-4-one (flavone) scaffolds were less potent. Crucially, these studies revealed a very constrained structure-activity relationship at the 2-position of the benzopyranone and pyrimido[2,1-a]isoquinolin-4-one pharmacophore, with only a 2-morpholino or 2-(2'-methylmorpholino) group being tolerated at this position. More detailed biological studies conducted with the most potent inhibitor NU7163 (48; IC(50) = 0.19 microM) demonstrated ATP-competitive DNA-PK inhibition, with a K(i) value of 24 nM, and 48 exhibited selectivity for DNA-PK compared with the related enzymes ATM, ATR, mTOR, and PI 3-K (p110alpha). Compound 48 sensitized the HeLa human tumor cell line to the cytotoxic effects of ionizing radiation in vitro, a dose modification factor of 2.3 at 10% survival being observed with an inhibitor concentration of 5 microM. This study identified these structural classes as novel DNA-PK inhibitors and delineated initial structure-activity relationships against DNA-PK.
Role of Chemokines and Their Receptors in Cancer (2009)

Metastases are the cause of 90% of human cancer deaths. The current treatment of cancer with chemo,- and/or radiotherapy is based on cell death by DNA damage neglecting the fact that cancer cell invasion into surrounding tissues and metastasizing are fundamental features of neoplasms and the major reason for treatment failure. Metastasis is the result of several sequential steps and represents a highly organized, non-random, and organ-selective process. A number of in vitro and in vivo models show that tumor cells use chemokine-mediated mechanisms during this metastasizing process, comparable to those observed in the regulation of leukocyte trafficking. Furthermore, chemokines modulate tumor behavior such as the regulation of tumor-associated angiogenesis, activation of host tumor-specific immunological responses, and direct stimulation of tumor cell proliferation in an autocrine fashion. These findings may lead to new drugs that target chemokines or their receptors and will likely be of great additional value for treatment of cancer patients.
Fibre optic oxygen sensor based on fluorescence quenching of evanescent-wave excited ruthenium complexes in sol–gel derived porous coatings (1993)

A simple, low-cost technique for the fabrication of optical sensors for oxygen is described and preliminary results obtained using these sensors are reported. The technique is based on coating a declad portion of an optical fibre with a microporous glass film prepared by the sol–gel process. A ruthenium complex [RuII–tris-(2,2′-bipyridine) or RuII–tris(4,7-diphenyl-1,10-phenanthroline)] is trapped in the nanometre-scale cage-like structure of the porous film. In this sensor configuration the complex is excited by the evanescent field of the 488 nm radiation guided by the optical fibre. The luminescence from such complexes is known to be quenched by oxygen and the sensors exhibit repeatable quenching behaviour when exposed to various concentrations of oxygen. The ratio R=I0/I100 where I0 and I100 represent the detected signals from a sensor exposed to 100% nitrogen and 100% oxygen, respectively, is used as a measure of the sensitivity of the sensor. Sensors based on the diphenylphenanthroline complex exhibit greater sensitivity than those based on the bipyridine complex, in accordance with theoretical predictions. More importantly, however, the design potential of the sol–gel process for sensor fabrication is demonstrated by the achievement of a substantial increase in R when the process parameters are adjusted to increase the pore volume.
A Markov Multi-Phase Transferable Belief Model: an Application for Predicting Data Exfiltration Apts (2013)

eXfiltration Advanced Persistent Threats (XAPTs) increasingly account for incidents concerned with intelligence information gathering by malicious adversaries. This research exploits the multi-phase nature of an XAPT, mapping its phases into a cyber attack kill chain. A novel Markov Multi-Phase Transferable Belief Model (MM-TBM) is proposed and demonstrated for fusing incoming evidence from a variety of sources which takes into account conflicting information. The MM-TBM algorithm predicts a cyber attacker's actions against a computer network and provides a visual representation of their footsteps.