gebhart/patrepeval huggingface dataset:
Caveolin-1 regulates EGFR signalling in MCF-7 breast cancer cells and enhances gefitinib-induced tumor cell inhibition (2009)

Caveolin-1, an essential protein constituent of caveolae, is involved in cell signaling through its association with various signaling molecules. Epidermal growth factor receptor (EGFR) interacts directly with caveolin-1 and this interaction functionally regulates EGFR tyrosine kinase activity. In this report we investigated the role of caveolin-1 overexpression on EGFR signaling in MCF-7 breast cancer cell line. We demonstrate here that although total EGFR expression levels are similar, EGFR phosphorylation is diminished in MCF-7/CAV1 cells compared to parental MCF-7 cells. In addition, MCF-7/CAV1 cells exhibit higher total and activated Akt levels. Moreover, MCF-7/CAV1 cells stimulated with EGF display higher EGFR and Akt phosphorylation associated with enhanced proliferative and motility rates, compared to MCF-7 cells. Pretreatment with gefitinib inhibits EGF-induced stimulation of both EGFR and downstream Akt and MAPK more efficiently in MCF-7/CAV1 than in MCF-7 cells. In accordance, EGF-induced proliferation and migration is more effectively suppressed with gefinitib in MCF-7/CAV1 compared to parental cells. In conclusion these results suggest that caveolin-1 overexpression in MCF-7 breast cancer cell line modulates EGFR activation levels and EGF-induced EGFR signalling. This is associated with enhanced antitumor effects of gefitinib suggesting a role for EGFR inhibition in caveolin-1 overexpressing breast cancers.
The role of <i>clp</i>-regulated factors in antagonism against <i>Magnaporthe poae</i> and biological control of summer patch disease of Kentucky bluegrass by <i>Lysobacter enzymogenes </i>C3 (2005)

A global regulator was previously identified in Lysobacter enzymogenes C3, which when mutated, resulted in strains that were greatly reduced in the expression of traits associated with fungal antagonism and devoid of biocontrol activity towards bipolaris leaf-spot of tall fescue and pythium damping-off of sugarbeet. A clp gene homologue belonging to the crp gene family was found to globally regulate enzyme production, antimicrobial activity, and biological control activity expressed by Lysobacter enzymogenes C3 (Kobayashi et al. 2005). Here, we report on the expansion of the biocontrol range of L. enzymogenes C3 to summer patch disease caused by Magnaporthe poae. The clp- mutant strain 5E4 was reduced in its ability to suppress summer patch disease compared with the wild-type strain C3 and was completely devoid of antifungal activity towards M. poae. Furthermore, cell suspensions of 5E4 were incapable of colonizing M. poae mycelium in a manner that was distinct for C3. Strain C3 demonstrated biosurfactant activity in cell suspensions and culture filtrates that was associated with absorption into the mycelium during the colonization process, whereas 5E4 did not. These results describe a novel interaction between bacteria and fungi that intimates a pathogenic relationship.
Channel estimation for TD-LTE system (2011)

TD-LTE has become one of the mainstream technology for 4G. Novel technologies such as orthogonal frequency division multiplexing (OFDM) and multiple input, multiple output (MIMO) have been selected for LTE downlink to improve the performance of the current wireless communication systems. In TD-LTE system, channel estimation is an important basis to obtain channel information, proceed channel equalization and choose transmission modes. Some channel estimation algorithms for LTE downlink, i.e. the least square (LS), liner minimum mean squared error (LMMSE) and singular value decomposition (svd) are studied in this paper and the simulation of their performance is given.
A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs (2018)

Detection of DNA methylation in the genome has been possible for decades; however, the ability to deliberately and specifically manipulate local DNA methylation states in the genome has been extremely limited. Consequently, this has impeded our understanding of the direct effect of DNA methylation on transcriptional regulation and transcription factor binding in the native chromatin context. Thus, highly specific targeted epigenome editing tools are needed to address this. Recent adaptations of genome editing technologies, including fusion of the DNMT3A DNA methyltransferase catalytic domain to catalytically inactive Cas9 (dC9-D3A), have aimed to alter DNA methylation at desired loci. Here, we show that these tools exhibit consistent off-target DNA methylation deposition in the genome, limiting their capabilities to unambiguously assess the functional consequences of DNA methylation. To address this, we developed a modular dCas9-SunTag (dC9Sun-D3A) system that can recruit multiple DNMT3A catalytic domains to a target site for editing DNA methylation. dC9Sun-D3A is tunable, specific, and exhibits much higher induction of DNA methylation at target sites than the dC9-D3A direct fusion protein. Importantly, genome-wide characterization of dC9Sun-D3A binding sites and DNA methylation revealed minimal off-target protein binding and induction of DNA methylation with dC9Sun-D3A, compared to pervasive off-target methylation by dC9-D3A. Furthermore, we used dC9Sun-D3A to demonstrate the binding sensitivity to DNA methylation for CTCF and NRF1 in situ. Overall, this modular dC9Sun-D3A system enables precise DNA methylation deposition with the lowest off-target DNA methylation levels reported to date, allowing accurate functional determination of the role of DNA methylation at single loci.
Photon sieve telescope (2005)

The creation of next generation, ultra-large space telescopes (>20m diameter) will require novel technologies. Many current concepts involve curved membrane reflectors but the problem is creating a diffraction-limited, three-dimensional surface. Here we present the idea of using a flat diffractive element which requires no out-of-plane deformation and is thus much simpler to deploy. The primary is a photon sieve--a diffractive element consisting of a large number of precisely positioned holes distributed over a flat surface. Photon sieves can be simply designed to any conic, apodization and operating bandwidth. The photon sieve is easier to fabricate than the better known Fresnel zone plate as a single substrate can be used since there are no connected regions requiring support. Presented here are results of prototypes capable of diffraction-limited imaging over wide fields and useful bandwidths.
NEW YORK ACADEMY OF MEDICINE, SECTION OF PEDIATRICS (1940)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Pediatrics HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Pediatrics journal
A Pulsed Electromagnetic Field Therapy Device for Non-Specific Low Back Pain: A Pilot Randomized Controlled Trial (2019)

Aerotel Ltd.
Adaptive Tuning of Switched-Mode Power Supplies Operating in Discontinuous and Continuous Conduction Modes (2009)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> This paper presents an approach to adaptive tuning of voltage-mode digital controllers for switched-mode power supplies in the presence of large signal changes from discontinuous conduction mode (DCM) to continuous conduction mode (CCM) and <emphasis emphasistype="italic">vice versa</emphasis>. The approach is capable of maintaining a high-performance control loop without the stability issues related to DCM-to-CCM mode transitions. The adaptive tuner, modeled as a multiple-input–multiple-output (MIMO) controller, is designed to continuously adjust the parameters of a PID compensator such that crossover frequency and phase margin measured by the digital controller match desired values. A simplified design procedure for the adaptive tuning system is proposed that reduces the small-signal MIMO design into two independent single-input, single-output control loops. Simulation results are given showing that despite wide uncertainty in the power stage, the adaptive tuning system still converges to the desired stability margins. Experimental results are given using an 11- to 5-V, 45-W, DCM/CCM buck converter as a test bed. </para>
Thinning of the Cerebral Cortex in Aging (2004)

The thickness of the cerebral cortex was measured in 106 non-demented participants ranging in age from 18 to 93 years. For each participant, multiple acquisitions of structural T1-weighted magnetic resonance imaging (MRI) scans were averaged to yield high-resolution, high-contrast data sets. Cortical thickness was estimated as the distance between the gray/white boundary and the outer cortical surface, resulting in a continuous estimate across the cortical mantle. Global thinning was apparent by middle age. Men and women showed a similar degree of global thinning, and did not differ in mean thickness in the younger or older groups. Age-associated differences were widespread but demonstrated a patchwork of regional atrophy and sparing. Examination of subsets of the data from independent samples produced highly similar age-associated patterns of atrophy, suggesting that the specific anatomic patterns within the maps were reliable. Certain results, including prominent atrophy of prefrontal cortex and relative sparing of temporal and parahippocampal cortex, converged with previous findings. Other results were unexpected, such as the finding of prominent atrophy in frontal cortex near primary motor cortex and calcarine cortex near primary visual cortex. These findings demonstrate that cortical thinning occurs by middle age and spans widespread cortical regions that include primary as well as association cortex.
Slicing-based test case generation from UML activity diagrams (2009)

UML diagrams are important design and modeling artifacts. These diagrams can also be used to generate test cases. We present a novel test case generation method that is based on dynamic slicing of UML activity diagrams. We use flow dependence graph (FDG) of an activity diagram to generate dynamic slices. Dynamic slices are created using an edge marking method. Slices are constructed corresponding to each conditional predicate on activity edges and test cases are automatically generated with respect to each slice. Our generated test cases satisfy path coverage criterion. Our test data generation scheme is automatic and the test data is generated considering the slice condition. We have implemented our approach to realize a prototype tool.
Crosslinked blended poly(vinyl alcohol)/N-methylol nylon-6 membranes for the pervaporation separation of ethanol-water mixtures (1998)

Crosslinked blended membranes of poly(vinyl alcohol) (PVA) and N-methylol nylon-6 were prepared either by thermal crosslinking at 180°C or by chemical crosslinking with maleic acid. The pervaporation performance for the separation of ethanol–water mixtures of these membranes was investigated in terms of feed concentration, PVA content, and crosslinking agent content. The pervaporation performance of two differently crosslinked membranes was strongly influenced by the nature of the crosslinkage. Significant improvement in the pervaporation separation index can be achieved for chemically crosslinked membranes. From the comparison between the pervaporation and sorption tests, it is suggested that, for hydrophilic membranes, sorption properties dominate the pervaporation performance at feed solutions of higher water content, while diffusion properties govern at feed solutions of higher ethanol content. © 1998 John Wiley & Sons, Inc. J. Appl. Polym. Sci. 70: 317–327, 1998
Surface Roughening of Polystyrene and Poly(methyl methacrylate) in Ar/O2 Plasma Etching (2010)

Selectively plasma-etched polystyrene-block-poly(methyl methacrylate) (PS-b-PMMA) diblock copolymer masks present a promising alternative for subsequent nanoscale patterning of underlying films. Because mask roughness can be detrimental to pattern transfer, this study examines roughness formation, with a focus on the role of cross-linking, during plasma etching of PS and PMMA. Variables include ion bombardment energy, polymer molecular weight and etch gas mixture. Roughness data support a proposed model in which surface roughness is attributed to polymer aggregation associated with cross-linking induced by energetic ion bombardment. In this model, RMS roughness peaks when cross-linking rates are comparable to chain scissioning rates, and drop to negligible levels for either very low or very high rates of cross-linking. Aggregation is minimal for very low rates of cross-linking, while very high rates produce a continuous cross-linked surface layer with low roughness. Molecular weight shows a negligible effect on roughness, while the introduction of H and F atoms suppresses roughness, apparently by terminating dangling bonds. For PS etched in Ar/O2 plasmas, roughness decreases with increasing ion energy are tentatively attributed to the formation of a continuous cross-linked layer, while roughness increases with ion energy for PMMA are attributed to increases in cross-linking from negligible to moderate levels.
Deterministic Networking Use Cases (2019)

This draft presents use cases from diverse industries which have in
common a need for deterministic flows. Deterministic in this
context means that such flows provide guaranteed bandwidth, bounded
latency, and other properties germane to the transport of time-
sensitive data. These use cases differ notably in their network
topologies and specific desired behavior, providing as a group broad
industry context for DetNet. For each use case, this document will
identify the use case, identify representative solutions used today,
and describe potential improvements that DetNet can enable. The Use
Case Common Themes section then extracts and enumerates the set of
common properties implied by these use cases.
Discovery of Protein Disulfide Isomerase P5 Inhibitors that Reduce the Secretion of MICA from Cancer Cells (2014)

In order to regulate the activity of P5, which is a member of the protein disulfide isomerase family, we screened a chemical compound library for P5-specific inhibitors, and identified two candidate compounds (anacardic acid and NSC74859). Interestingly, anacardic acid inhibited the reductase activity of P5, but did not inhibit the activity of protein disulfide isomerase (PDI), thiol-disulfide oxidoreductase ERp57, or thioredoxin. NSC74859 inhibited all these enzymes. When we examined the effects of these compounds on the secretion of soluble major histocompatibility complex class-I-related gene A (MICA) from cancer cells, anacardic acid was found to decrease secretion. In addition, anacardic acid was found to reduce the concentration of glutathione up-regulated by the anticancer drug 17-demethoxygeldanamycin in cancer cells. These results suggest that anacardic acid can both inhibit P5 reductase activity and decrease the secretion of soluble MICA from cancer cells. It might be a novel and potent anticancer treatment by targeting P5 on the surface of cancer cells.
Producing Nanoparticles Using Precipitation with Compressed Antisolvent (2007)

The application of supercritical fluid processing in pharmaceutical research is increasing, particularly in the field of particle formation for drug delivery systems, mainly due to advantages over the conventional particle formation in terms of improved control, flexibility, ease of operation, and level of toxicity from the presence of organic solvents and contaminants. Particle formation of salicylic acid using supercritical carbon dioxide (CO2) was investigated using the precipitation with compressed antisolvent (PCA) technique using ethanol and acetone as solvents. The solubility of salicylic acid (SA) in supercritical CO2 was measured in its pure form and with modifiers, at 45 and 55 °C over a pressure range of 95−250 bar. The output of the study gave promising conditions in particle formation by adjusting parameters based on the phase diagram obtained. Particles in the nanorange can be produced in the higher supersaturation region without changing the nozzle size of the PCA system. All the experimental conditions investigated here gave an average particle size of approximately 100 nm, with the smallest average particle size of 63.35 nm achieved by using 1% acetone modifying condition at 45 °C. The largest average particle size produced was 102.75 nm at the condition with 1% ethanol operating under a temperature of 55 °C. A dissolution experiment was also carried out to show significant improvement of drug dissolution rate with the smaller particle size.
[Effects of mast cells on degradation of collagen fibers in dimethylnitrosamine-induced hepatic fibrosis of rat]. (2012)

In the DMN-induced rat liver fibrosis model, mast cell may be an integral player in the pathogenesis of liver fibrosis and may contribute to the degradation of fibrosis by synthesizing and secreting MMP-2.
Macroporous Supported Cu–V Oxide as a Promising Substitute of the Pt Catalyst for Sulfuric Acid Decomposition in Solar Thermochemical Hydrogen Production (2012)

The macroporous supported Cu–V oxide prepared by a novel dissolution–reprecipitation process was found to be the first example of a promising substitute of Pt catalysts for sulfuric acid decomposition at moderate temperatures (∼600 °C), which is required in solar thermochemical hydrogen production. Stepwise impregnation of Cu(NO3)2 and NH4VO3 onto 3-D ordered mesoporous SiO2, and subsequent heating at 650 °C yielded the deposition of copper pyrovanadate (Cu2V2O7, melting point: 780 °C) not only in mesopores but also on the external surface. Thermal aging at 800 °C caused the congruent melting of Cu2V2O7 followed by smooth penetration of the melt into mesopores and homogeneous covering of cavity walls. Because of the solubility of SiO2 into the molten vanadate, dissolution–reprecipitation should be equilibrated to allow substantial structural conversion from mesoporous to macroporous SiO2 frameworks. The resulting macroporous catalyst consisting of highly dispersed thin layers of active Cu2V2O7 is considered efficient for catalytic reactions and the mass transfer of reactants and products in the presence of high-concentration vapors.
Studies on Zn uptake and influence of Zn and Fe on chlorophyll development in young maize cultivars (1985)

Abstract Maize (Zea mays L.) seeds of hybrid Ganga‐2 and its parent cultivars were germinated and allowed to absorb Zn labelled ZnCl2 , ZnEDTA or ZnEDDHA for 24 hours. The translocation of Zn in seedlings raised from them was followed on 5, 7 and 10 days. It was found that while the endosperm Zn content decreased rapidly, there was no significant increase in Zn transported to scutellum, root and shoot. There was no difference between Zn treatments as well as between the cultivars. The results demonstrated the poor mobility of Zn in maize during early growth. Further studies showed that the pretreatment of seeds with ZnEDTA and low amount of Fe enhanced chlorophyll content in 10 day old seedlings. No hybrid vigor was observed either in Zn translocation, or effects of Zn and Fe on chlorophyll synthesis.
Abstract 659: Overexpression of TPX2 accelerates cell cycle progression in melanoma and is an indicator of response to Aurora A kinase inhibitors (2010)

Abstract There is a tremendous need to identify more effective treatments for metastatic melanoma. In order to detect genetic changes in melanoma that might facilitate the development of novel therapies, we performed aCGH in melanoma cell lines and tumors and identified that Targeting Protein for Xklp2 (TPX2) was the most commonly amplified gene on 20q11.21, a region of frequent gain. Gene expression profiling studies also demonstrated overexpression of TPX2, suggesting a previously unknown role in the development of melanoma. TPX2 is a microtubule-associated protein that regulates bipolar spindle assembly and centrosome replication; the TPX2 - Aurora A interaction is essential for accurate segregation of chromosome and mitosis progression. We evaluated a potential role for TPX2 overexpression in melanoma using TPX2 overexpressing melanoma cell lines as a model. TPX2 overexpressing cell lines display accelerated cell cycle progression, increased CDK1/CDK2 activity, cyclin A/B1 levels, and constitutive activation and overexpression of Aurora A. We depleted TPX2 using shRNA to further dissect its role in melanoma. In unsynchronized cells, TPX2 depletion causes prolonged mitotic arrest accompanied by persistent activation of the spindle-assembly checkpoint (SAC) and caspase-3, and subsequent caspase-dependent cell death. In contrast, with G1/S synchronization, TPX2 depletion decreases S phase entry and delays S-G2-M phase transition followed by marked cell death. Accordingly, TPX2 depletion was associated with decreased CDK1 activity but not CDK2 activity, and activation of caspase and low activation of the SAC. Our data demonstrate that TPX2 overexpression is required for increased CDK1-cyclinB1 activity, thus accelerating cell cycle progression. Accordingly, TPX2-overexpressing melanoma cell lines are dependent on TPX2 for proliferation, whereas non-overexpressing cell lines and melanocytes are not. Additionally, TPX2-overexpressing cell lines are 3-10 time more sensitive to an Aurora A kinase inhibitor (VX680) than non-overexpressing cell line, suggesting that TPX2-activating Aurora A kinase could become a novel therapeutic target for treatment of melanoma with highly expressed TPX2, Finally, TPX2-IHC staining demonstrated that a significantly increased expression of TPX2 in primary and metastatic melanoma as compared to begin nevi, suggesting it is a marker of progression. Thus, we have identified TPX2 as a novel gene upregulated in melanoma. Overexpression of TPX2 may be a marker of tumors sensitive to Aurora A inhibitors allowing patient stratification for optimal therapeutic selection. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 659.