gebhart/patrepeval huggingface dataset:
Sensitive Determination of Microbial Growth by Nucleic Acid Staining in Aqueous Suspension (2006)

The determination of cell numbers or biomass in laboratory cultures or environmental samples is usually based on turbidity measurements, viable counts, biochemical determinations (e.g., protein and lipid measurements), microscopic counting, or recently, flow cytometric analysis. In the present study, we developed a novel procedure for the sensitive quantification of microbial cells in cultures and most-probable-number series. The assay combines fluorescent nucleic acid staining and subsequent fluorescence measurement in suspension. Six different fluorescent dyes (acridine orange, DAPI [4',6'-diamidino-2-phenylindole], ethidium bromide, PicoGreen, and SYBR green I and II) were evaluated. SYBR green I was found to be the most sensitive dye and allowed the quantification of 50,000 to up to 1.5 x 10(8) Escherichia coli cells per ml sample. The rapid staining procedure was robust against interference from rRNA, sample fixation by the addition of glutaric dialdehyde, and reducing agents such as sodium dithionite, sodium sulfide, and ferrous sulfide. It worked well with phylogenetically distant bacterial and archaeal strains. Excellent agreement with optical density measurements of cell increases was achieved during growth experiments performed with aerobic and sulfate-reducing bacteria. The assay offers a time-saving, more sensitive alternative to epifluorescence microscopy analysis of most-probable-number dilution series. This method simplifies the quantification of microbial cells in pure cultures as well as enrichments and is particularly suited for low cell densities.
Specific Roles of NMDA and AMPA Receptors in Direction-Selective and Spatial Phase-Selective Responses in Visual Cortex (2001)

Cells in the superficial layers of primary visual cortex (area 17) are distinguished by feedforward input from thalamic-recipient layers and by massive recurrent excitatory connections between neighboring cells. The connections use glutamate as transmitter, and the postsynaptic cells contain both NMDA and AMPA receptors. The possible role of these receptor types in generating emergent responses of neurons in the superficial cortical layers is unknown. Here, we show that NMDA and AMPA receptors are both involved in the generation of direction-selective responses in layer 2/3 cells of area 17 in cats. NMDA receptors contribute prominently to responses in the preferred direction, and their contribution to responses in the nonpreferred direction is reduced significantly by GABAergic inhibition. AMPA receptors decrease spatial phase-selective simple cell responses and generate phase-invariant complex cell responses.
Vacuum Consolidation Method - Worldwide Practice and the Latest Improvement in Japan (2006)

Vacuum consolidation method is a technique of applying vacuum suction to an isolated soil mass to reduce the atmospheric pressure in it, thus by the way of reducing the pore water pressure in the soil the effective stress is increased without changing the total stress. By improving construction techniques, as well as developing analytical methods for designing, the technique has become an effective and economical method for soft ground improvement and capable to conduct in various site conditions. Researches are also extended to other application fields such as acceleration dewatering and consolidation of fluid-like materials. Conducting of numerous vacuum consolidation projects has been tried in many countries in the world, through that practical experiences are accumulated, which would have significant importance in improving its efficacy and advancing the technique. For the purpose of gaining experience, numbers of published documents and research papers on vacuum consolidation practice from leading countries in this field of technology have been reviewed. This paper summarizes and compares various vacuum consolidation systems with emphasizing on system specification and configuration, design and construction practice, and introduces the newest improvement of this technology in Japan.
Peripheral blood stem cell support reduces the toxicity of intensive chemotherapy for children and adolescents with metastatic sarcomas (2002)

PBSC-supported multicycle chemotherapy is a feasible method to increase chemotherapy DI for pediatric patients with metastatic sarcomas. Although the CR rate compared favorably with previously reported response rates, the 2-year EFS rate was similar to that achieved with other intensive regimens.
INSURANCE INSTITUTE FOR HIGHWAY SAFETY SIDE IMPACT CRASHWORTHINESS EVALUATION PROGRAM: IMPACT CONFIGURATION AND RATIONALE (2003)

The Insurance Institute for Highway Safety (IIHS) has begun a new side impact crashworthiness evaluation program using tests that represent impacts from large pickup trucks or sport utility vehicles. Such vehicles are increasingly common in the North American fleet and often are the striking vehicles in side impacts with serious injuries. Earlier reports document the research underpinning the design of the new IIHS moving deformable barrier (MDB) and the selection of the SID-IIs dummy for the driver and left rear passenger positions. In this report, research is discussed in which alternative mass (1,500 or 1,900 kg), impact speed (48.3 or 50.0 km/h), and approach angle (crabbed or perpendicular) of the MDB were investigated. Impact speed affected dummy injury measures and kinematics more than mass or approach angle. Based on these results, the impact configuration for the side impact program specifies a 1,500 kg MDB, a perpendicular impact into the side of a stationary vehicle, and a test speed of 50.0 km/h. For the covering abstract see ITRD E825082.
Development of a rotary joint fluid coupling for Space Station Freedom (1991)

The design and development is described of a rotary joint fluid coupling for Space Station Freedom. The function of this fluid coupling is to transfer a heat rejection fluid between stationary and rotating interfaces with the Space Station thermal bus system. The design of this coupling incorporates a modular type design to allow maximum flexibility, two types of seals (mechanical face seals and shaft lip seals) for redundancy, materials with excellent ammonia compatibility, and coatings that enhance seal wear resistance and increased protection against corrosion. This design was thoroughly tested and met all design requirements. Potential applications of this hardware include uses in gun turrets, coal slurries, farming equipment, and any other applications that require the low leakage transfer of fluids between stationary and rotating interfaces.
Metabolic flux analysis and metabolic engineering of microorganisms (2007)

Recent advances in metabolic flux analysis including genome-scale constraints-based flux analysis and its applications in metabolic engineering are reviewed. Various computational aspects of constraints-based flux analysis including genome-scale stoichiometric models, additional constraints used for the improved accuracy, and several algorithms for identifying the target genes to be manipulated are described. Also, some of the successful applications of metabolic flux analysis in metabolic engineering are reviewed. Finally, we discuss the limitations that need to be overcome to make the results of genome-scale flux analysis more realistically represent the real cell metabolism.
Summarizing popular music via structural similarity analysis (2004)

We present a framework for summarizing digital media based on structural analysis. Though these methods are applicable to general media, we concentrate here on characterizing the repetitive structure in popular music. In the first step, a similarity matrix is calculated from interframe spectral similarity. Segment boundaries, such as verse-chorus transitions, are found by correlating a kernel along the diagonal of the matrix. Once segmented, spectral statistics of each segment are computed. In the second step, segments are clustered, based on the pairwise similarity of their statistics, using a matrix decomposition. Finally, the audio is summarized by combining segments representing the clusters most frequently repeated throughout the piece. We present results on a small corpus showing more than 90% correct detection of verse and chorus segments.
Deep Learning IP Network Representations (2018)

We present DIP, a deep learning based framework to learn structural properties of the Internet, such as node clustering or distance between nodes. Existing embedding-based approaches use linear algorithms on a single source of data, such as latency or hop count information, to approximate the position of a node in the Internet. In contrast, DIP computes low-dimensional representations of nodes that preserve structural properties and non-linear relationships across multiple, heterogeneous sources of structural information, such as IP, routing, and distance information. Using a large real-world data set, we show that DIP learns representations that preserve the real-world clustering of the associated nodes and predicts distance between them more than 30% better than a mean-based approach. Furthermore, DIP accurately imputes hop count distance to unknown hosts (i.e., not used in training) given only their IP addresses and routable prefixes. Our framework is extensible to new data sources and applicable to a wide range of problems in network monitoring and security.
A Layered Grammar of Graphics (2009)

A grammar of graphics is a tool that enables us to concisely describe the components of a graphic. Such a grammar allows us to move beyond named graphics (e.g., the “scatterplot”) and gain insight into the deep structure that underlies statistical graphics. This article builds on Wilkinson, Anand, and Grossman (2005), describing extensions and refinements developed while building an open source implementation of the grammar of graphics for R, ggplot2. The topics in this article include an introduction to the grammar by working through the process of creating a plot, and discussing the components that we need. The grammar is then presented formally and compared to Wilkinson’s grammar, highlighting the hierarchy of defaults, and the implications of embedding a graphical grammar into a programming language. The power of the grammar is illustrated with a selection of examples that explore different components and their interactions, in more detail. The article concludes by discussing some perceptual issues, and thinking about how we can build on the grammar to learn how to create graphical “poems.” Supplemental materials are available online.
Sympathoinhibitory and Depressor Effects of Amlodipine in Spontaneously Hypertensive Rats (2003)

The authors examined whether central actions contribute to the hypotensive effects of peripherally administered amlodipine, a lipophilic dihydropyridine with slow onset and long duration of action. After 5 to 6 weeks of high (8%, H-Na) or regular (0.6%, R-Na) salt intake, changes in renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP), and heart rate (HR) were recorded at rest and in response to intravenous (iv) and intracerebroventricular (icv) injection, and prolonged iv infusion of amlodipine, in conscious spontaneously hypertensive rats (SHR). Iv injection of amlodipine at 50 to 100 microg/kg decreased MAP but increased RSNA and HR in a dose-related manner. In contrast, icv injection of amlodipine at 10 to 50 microg/kg caused parallel decreases in MAP, RSNA, and HR. Iv infusion of amlodipine at 50 microg/kg per hour for 3 hours followed by 100 microg/kg per hour for 2 hours also decreased in parallel RSNA, MAP, and HR. Maximal decreases in RSNA, MAP, and HR in response to icv injection and iv infusion were significantly larger in SHR on H-Na versus R-Na. All responses lasted at least 1 hour following iv and icv injection, or after the termination of iv infusion of amlodipine. These data suggest that in SHR during prolonged iv infusion, amlodipine appears to cross the blood-brain barrier, block brain l-type Ca2+ channels, and decrease sympathetic outflow and thereby BP. Central actions may prevail during iv infusion of amlodipine at low rates, and the decrease in BP is associated with sympathoinhibition. High salt intake markedly enhances its sympathoinhibitory action, likely through central mechanisms.
Sudden Increase in Isolation of Group B Streptococci, Serotype V, Is Not Due to Emergence of a New Pulsed-Field Gel Electrophoresis Type (1998)

Until recently, group B streptococcus, serotype V (GBS-V), was an infrequent cause of disease. It is now recognized as a significant cause of infections in both children and adults. To determine if this increase was due to the recent introduction and spread of a single clone of GBS-V, we analyzed, by pulsed-field gel electrophoresis (PFGE), the SmaI chromosomal DNA digests of 45 bacteria: 41 isolated from human infections between 1986 and 1996 in the United States, 2 from human infections in Argentina, and 2 from naturally infected mice. Seventeen patterns were found and arbitrarily designated patterns A to Q. Pattern N constituted 24 (53%) of the isolates and was found in all of the years tested and from all surveillance areas, as well as in both isolates from Argentina, and was very similar to the GBS-V isolated from a mouse. Pattern P was found in three isolates, pattern F was found in two, and the remaining patterns were found in one isolate each. We concluded that the majority of isolates of GBS-V are of one PFGE subtype and that this subtype was predominate before the increase in disease caused by GBS-V and that GBS-V disease is caused by several different subtypes.
Restless Legs Syndrome Treatment with Dopaminergic Drugs (1987)

Sixteen patients with symptoms of restless legs syndrome and resulting insomnia are included in this study. They were nine women and seven men with a mean age of 50.8 years and with a mean duration of symptoms of 6.3 years. The purpose of the study is to determine the drugs that are useful for the treatment of restless legs syndrome. In 13 patients L-Dopa plus benserazide, in two patients bromocriptine, and in the remaining one patient piribedil were used orally at night approximately 1 h before bedtime. Compared with placebo these dopaminergic drugs decreased the times of waking up and staying awake periods at a statistically significant level (p changed between 0.025 and 0.01, t test). In this susceptibility of CNS there is dysfunction of the dopamine system triggered by sleep, and resting and dopaminergic potentiation by drugs affect the symptoms.
Accelerated Stability Testing of a Clobetasol Propionate Loaded Nanoemulsion as per ICH Guideline (2013)

The physical and chemical degradation of drugs may result in altered therapeutic efficacy and even toxic effects. Therefore, the objective of this work was to study the stability of clobetasol propionate (CP) in a nanoemulsion. The nanoemulsion formulation containing CP was prepared by the spontaneous emulsification method. For the formulation of the nanoemulsion, Safsol, Tween 20, ethanol, and distilled water were used. The drug was incorporated into an oil phase in 0.05% w/v. The lipophilic nature of the drug led to the O/W nanoemulsion formulation. This was characterized by droplet size, pH, viscosity, conductivity, and refractive index. Stability studies were performed as per ICH guidelines for a period of three months. The shelf life of the nanoemulsion formulation was also determined after performing accelerated stability testing (40°C ± 2°C and 75% ± 5% RH). We also performed an intermediate stability study (30°C ± 2°C/65% RH ± 5% RH). It was found that the droplet size, conductivity, and refractive index were slightly increased, while the viscosity and pH slightly decreased at all storage conditions during the 3-month period. However, the changes in these parameters were not statistically significant (p≥0.05). The degradation (%) of the optimized nanoemulsion of CP was determined and the shelf life was found to be 2.18 years at room temperature. These studies confirmed that the physical and chemical stability of CP were enhanced in the nanoemulsion formulation.
Instantaneous polarization attributes in the time–frequency domain and wavefield separation (2005)

ABSTRACT We introduce a method of wavefield separation from multicomponent data sets based on the use of the continuous wavelet transform. Our method is a further generalization of the approach proposed by Morozov and Smithson, in that by using the continuous wavelet transform, we can achieve a better separation of wave types by designing the filter in the time–frequency domain. Furthermore, using the instantaneous polarization attributes defined in the wavelet domain, we show how to construct filters tailored to separate different wave types (elliptically or linearly polarized), followed by an inverse wavelet transform to obtain the desired wave type in the time domain. Using synthetic and experimental data, we show how the present method can be used for wavefield separation.
Metformin Suppresses Colorectal Aberrant Crypt Foci in a Short-term Clinical Trial (2010)

The biguanide metformin is widely used for treating diabetes mellitus. We previously showed the chemopreventive effect of metformin in two rodent models of colorectal carcinogenesis. However, besides epidemiologic studies, little is known about the effects of metformin on human colorectal carcinogenesis. The objective of this pilot study was to evaluate the chemopreventive effect of metformin on rectal aberrant crypt foci (ACF), which are an endoscopic surrogate marker of colorectal cancer. We prospectively randomized 26 nondiabetic patients with ACF to treatment with metformin (250 mg/d, n = 12) or no treatment (control, n = 14); 23 patients were evaluable for end point analyses (9 metformin and 14 control); the two groups were similar in ACF number and other baseline clinical characteristics. Magnifying colonoscopy determined the number of rectal ACF in each patient at baseline and after 1 month in a blinded fashion (as were all laboratory end point analyses). We also examined proliferative activity in colonic epithelium (via proliferating cell nuclear antigen labeling index) and apoptotic activity (via terminal deoxynucleotidyl transferase dUTP nick-end labeling). At 1 month, the metformin group had a significant decrease in the mean number of ACF per patient (8.78 +/- 6.45 before treatment versus 5.11 +/- 4.99 at 1 month, P = 0.007), whereas the mean ACF number did not change significantly in the control group (7.23 +/- 6.65 versus 7.56 +/- 6.75, P = 0.609). The proliferating cell nuclear antigen index was significantly decreased and the apoptotic cell index remained unaltered in normal rectal epithelium in metformin patients. This first reported trial of metformin for inhibiting colorectal carcinogenesis in humans provides preliminary evidence that metformin suppresses colonic epithelial proliferation and rectal ACF formation in humans, suggesting its promise for the chemoprevention of colorectal cancer.
Quantitative and functional assay of MDR1/P170-mediated MDR in ascites cells of patients with ovarian cancer. (2005)

Direct detection of the expression level and function of MDR1/Pgp in the ascites provide useful information for the more efficient treatment of malignant diseases by proper adjustment of the chemotherapeutic protocol.
Towards Fault Propagation Analysis in Cloud Computing Ecosystems (2014)

Nowadays, Cloud Computing is a fundamental paradigm that provides computational resources as a service, on which users heavily rely. Cloud computing infrastructures behave as an ecosystem, where several actors play a crucial role. Unfortunately Cloud Computing Ecosystems (CCEs) are often affected by outages, such as those experienced by Amazon Web Service in the last years, that result from component faults that propagate through the whole CCE. Thus, there is still a need for approaches to improve CCEs' reliability. This paper discusses both existing approaches and open challenges for the dependability evaluation of CCEs, and the need for novel techniques and methodologies to prevent fault propagation within CCEs as a whole.
Advances in standoff detection of trace explosives by infrared photo-thermal imaging (2010)

A technique for stand-off detection of trace explosives using infrared (IR) photo-thermal (PT) imaging, remote explosives detection (RED), is under development at the Naval Research Laboratory. In this approach, compact IR quantum cascade lasers (QCLs) tuned to strong absorption bands of trace explosives illuminate a surface of interest. An IR focal plane array is used to image the surface and detect any small increase in the thermal emission upon laser illumination. The technique has been previously demonstrated at several meters of stand-off distance indoors and in field tests with sensitivity to explosive traces as small as a single grain (~1 ng), while operating the lasers below the eye-safe intensity limit (100 mW/cm<sup>2</sup>) at the tested wavelengths. By varying the incident wavelength slightly, selectivity between TNT and RDX has been achieved. A complete test and analysis can be performed in less than 1 second. This manuscript critically examines components used with RED and demonstrates several improvements. These include QCL drive electronics for narrower spectral emission linewidth, fixed wavelength QCL packaging that optimizes spectral and spatial output, fiber-optic coupling for QCL beam steering and spatial filtering, cooled IR sensors that increase sensitivity and speed, tunable QCL sources that increase selectivity and extend the library of possible analytes, and dynamic PT signal processing that can increase sensitivity and speed. When considered in combination with the capabilities previously demonstrated for RED, and its capability to operate within eye-safety limits, this technology offers the potential for a wide area of applications relating to the detection of trace explosives on surfaces in both non-contact and stand-off configurations.
A 24GHz down-conversion mixer with low noise and high gain (2012)

In this paper, a down-converting mixer implemented in the TSMC 0.18-µm CMOS process technology for applications in the 24GHz band is presented. For suppressing the flicker noise generated in the switching stage of the conventional Gilbert cell topology, a current bleeding technique combined with negative resistance is adopted to improve the noise figure (NF) of the mixer. In addition, an inductor is utilized to resonate with the parasitic capacitances at the output of the transconductance (Gm) stage and thus increases the power conversion gain (CG) and the linearity of the proposed mixer. The chip size of the proposed mixer is 1.062 × 0.979 mm2, and the core circuit consumes a dc power of 5.65mW from the 1.8-V voltage supply. The measured CG and NF for the proposed 24GHz mixer are 8.376dB and 11.6dB, respectively. And the measured 1dB compression point (P1dB) is −8.4dBm at the 24GHz operating frequency.
Silver poisoning associated with an antismoking lozenge. (1978)

In this letter we have performed a structural analysis at the atomic scale of GaAs/AlGaAs quantum dots grown by droplet epitaxy. The shape, composition, and strain of the quantum dots and the AlGaAs matrix are investigated. We show that the GaAs quantum dots have a Gaussian shape and that minor intermixing of Al with the GaAs quantum dot takes place. A wetting layer with a thickness of less than one bilayer was observed. © 2010 American Institute of Physics. U7 - Export Date: 2 August 2010 U7 - Source: Scopus U7 - Art. No.: 062101