gebhart/patrepeval huggingface dataset:
Mechanisms of Nerve Capping Technique in Prevention of Painful Neuroma Formation (2014)

Nerve capping techniques have been introduced as a promising treatment modality for the treatment of painful neuroma with varied outcomes; however, its exact mechanism is still unknown. RhoA is one of the members of the RAS superfamily of GTPases that operate as molecular switches and plays an important role in peripheral nerve regeneration. Our aim was to investigate the structural and morphologic mechanisms by which the nerve capping technique prevents the formation of painful neuromas after neuroectomy. We also hoped to provide a theoretical basis for this treatment approach. An aligned nanofiber conduit was used for the capping procedure and the sciatic nerve of Sprague-Dawley rats was selected as the animal model. Behavioral analysis, extent of neuroma formation, histological assessment, expressions of pain markers of substance P and c-fos, molecular biological changes as well as ultrastructural features were investigated and compared with the findings in a no-capping control group. The formation of traumatic neuromas was significantly inhibited in the capping group with relatively "normal" structural and morphological features and no occurrence of autotomy and significantly lower expression of pain markers compared to the no-capping group. The gene expression of RhoA was consistently in a higher level in the capping group within 8 weeks after surgery. This study shows that capping technique will alter the regeneration state of transected nerves and reduce painful neuroma formation, indicating a promising approach for the treatment of painful neuroma. The initiation of the "regenerative brake" induced by structural as well as morphological improvements in the severed nerve is theorized to be most likely a key mechanism for the capping technique in the prevention of painful neuroma formation.
Increase in progressive motility and improved morphology of human spermatozoa following their migration through Percoll gradients (1986)

Human spermatozoa were separated on the basis of their motility in a discontinuous Percoll-gradient made up in tissue culture medium containing 10% (v/v) human serum (TCMS). Portions of ejaculates were placed on top of the gradients. After 3 h at 37 degrees C the bottom 1.5 ml was collected and the sperm washed free of the Percoll solution by centrifugation at 240 X g after dilution in TCMS. In this way the spermatozoa were separated from seminal fluid by means of the swimming rate of the sperm. When semen samples from normal men were used, total recovery of sperm after separated on a Percoll gradient was 21 +/- 2.3%. The progressive motility index increased by a factor of 15 +/- 1 when comparing separated samples with the same unseparated ejaculate, and the frequency of sperm with normal morphology increased from 60 to 85%. The improvements in these semen samples was attributable to the Percoll separation as the washing procedure itself was without effect. Using this method sperm of relatively unifirm motility and morphology can be collected. These may then be used for further biochemical and physiological studies. Improved sperm quality was also obtained when samples from patients with abnormal semen profiles were separated in this way, although the degree of improvement was much more variable than that obtained with semen from normal fertile men. This indicates that this method can be used in clinical practice in selected cases for the preparation of sperm for insemination or for in-vitro fertilization.
Calcium-induced intracellular cross-linking of lipocortin I by tissue transglutaminase in A431 cells. Augmentation by membrane phospholipids. (1991)

Covalently cross-linked multimers of lipocortin I are shown to be present in human epidermoid carcinoma A431 cells treated with epidermal growth factor or the calcium ionophore A23187. This intracellular cross-linking of lipocortin I is suggested to be mediated by the action of tissue transglutaminase, a Ca2(+)-dependent protein cross-linking enzyme. Cross-linking of lipocortin I competes with proteolytic digestion of the protein, and pretreatment of the cells with inhibitors for calpain (Ca2(+)-dependent intracellular protease) markedly enhanced the cross-linking of lipocortin I. Cross-linked lipocortin I is shown to be present in the soluble fraction of A431 cells as well as in the particulate fraction; a 34-kDa fragment of lipocortin I was solubilized successfully by plasmin digestion of the latter fraction. Immunofluorescence microscopy using specific antilipocortin-I antibody showed that cross-linked lipocortin I forms an envelope-like structure, which is not extracted with [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) or Triton X-100. In vitro incubation of purified lipocortin I with tissue transglutaminase resulted in the formation of covalently cross-linked lipocortin I dimer, tetramer, and so on. Amine incorporation and cross-linking studies using lipocortin I and its N-terminal truncated derivatives indicated that the cross-linking site is localized within the plasmin-susceptible N-terminal 29 amino acids of lipocortin I. The cross-linking of lipocortin I is shown to be accelerated more than 10 times by the addition of phosphatidylserine vesicles, on which lipocortin I molecules are most likely aligned in a conformation suitable for cross-linking. Collectively, these findings suggest that an increase of intracellular calcium concentration results in the attachment of lipocortin I onto the plasma membrane phospholipids through the C-terminal domain of the molecule where the membrane-bound lipocortin I is cross-linked by the action of tissue transglutaminase through the N-terminal domain.
On fault tolerance of reconfigurable arrays using spare processors (1991)

Addresses fault tolerance of a processor array that is reconfigurable by replacing faulty processors with spare processors. The fault tolerance of such a reconfigurable processor array depends on not only an algorithm for spare processor assignment but also an organization of spare processors in the reconfigurable array. The paper discusses a relationship between fault tolerance of reconfigurable arrays and their organizations of spare processors with respect to the smallest number of faulty processors for which the reconfigurable array cannot be failure-free as a processor array system no matter what reconfiguration is used. An optimum n*n reconfigurable array using 2n spare processors is presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Selfoc microlens with a spherical surface (1982)

This paper presents the light-focusing properties of a gradient-index rod lens with a spherical end surface which aims at increasing numerical aperture and decreasing lens aberration. Optimum refractive-index profiles for obtaining a smaller focused spot are derived, and the increase of numerical aperture is clarified. Experimental results are described; the Selfoc microlens with a spherical end surface realizes a focused spot below 2 microm in diameter.
Transfert d'une observation exploratoire à partir du guide européen : l'expérimentation de Chapelle-lez-Herlaimont (2008)

International audience
Chromosome aberration dosimetry using human lymphocytes in simulated partial body irradiation (1973)

The effect of X-irradiation on the yield of transformed cells in culture has been studied in human peripheral blood lymphocytes. Aberration yields are obtained for mixed cultures prepared from equal numbers of normal and irradiated lymphocytes and from pure cultures of irradiated cells. Comparison of data from these two types of culture show that the observed yield ranges from 90% of the expected at 50 rad to 11% at 700 rad. Poisson analysis demonstrates that for all doses, cells with high levels of chromosome structural damage are not selectively eliminated.
Laser‐based imaging for reverse engineering (2003)

In this paper, we explore the technical challenges to automatically generate computer‐aided design models of existing vehicle parts using laser range imaging techniques. We propose a complete system that integrates data acquisition and model reconstruction. We discuss methods to resolve the occlusion problem and the associated registration problem. We also present our reconstruction algorithm. This range image‐based, computer‐aided reverse engineering system has a potential for faster model reconstruction over traditional reverse engineering technologies. Finally, we present results derived from the system.
FAST RESIDUE-TO-BINARY CONVERSION USING BASE EXTENSION AND THE CHINESE REMAINDER THEOREM (2007)

Residue number systems are attractive due to their basic arithmetic operations, such as addition and multiplication, are carry-free; this facilitates low-power, high-speed implementations. But converting from residue to conventional notation is generally problematic. In this paper, we propose a high-speed architecture for reverse conversion; this is based on Base Extension and the Chinese Remainder Theorem.
Architecture and networking issues in satellite systems for personal communications (1994)

Abstract The paper expands on some specific aspects of personal communications via satellite, often–although not necessarily–envisaging extensive use of ‘small’ satellites, using orbits not necessarily coinciding with the geostationary one (36,000 km), but rather at 1000–11,000 km altitude. One key point to be borne in mind is the issue of internetworking between satellite and terrestrial systems. Network integration should be carefully implemented in order to comply with ITU future public land mobile telecommunication systems (FPLMTS) requirements, also accepted in the European Telecommunication Standards Institute (ETSI) universal mobile telecommunication systems (UMTS). In addition to some insight into the technology of small satellites and associated orbits, architectures and networking aspects of most relevant systems providing personal communication services (PCS) are addressed in some detail, and potential applications highlighted. Frequency spectrum allocation is addressed in the light of the 1992 World Administrative Radio Conference (WARC‐92), taking into account also multiple access problems and regulatory issues such as Federal Communications Commission (FCC) licensing and spectrum sharing debates. Some remarks on critical technologies and cost conclude the paper.
Domino reactions of 1-(2-alkoxyaryl)-3-akylprop-2-yn-1-ones with sodium sulfide leading to thiochromen-4-one derivatives (2012)

A simple and practical approach to thiochromen-4-one derivatives has been developed through domino reactions of 1-(2-alkoxyaryl)-3-akylprop-2-yn-1-ones and sodium sulfide nonahydrate. The domino reactions underwent sequential addition and cyclization via cleavage of aromatic C–O bonds.
Upconversion Nanomaterials: Synthesis, Mechanism, and Applications in Sensing (2012)

Upconversion is an optical process that involves the conversion of lower-energy photons into higher-energy photons. It has been extensively studied since mid-1960s and widely applied in optical devices. Over the past decade, high-quality rare earth-doped upconversion nanoparticles have been successfully synthesized with the rapid development of nanotechnology and are becoming more prominent in biological sciences. The synthesis methods are usually phase-based processes, such as thermal decomposition, hydrothermal reaction, and ionic liquids-based synthesis. The main difference between upconversion nanoparticles and other nanomaterials is that they can emit visible light under near infrared irradiation. The near infrared irradiation leads to low autofluorescence, less scattering and absorption, and deep penetration in biological samples. In this review, the synthesis of upconversion nanoparticles and the mechanisms of upconversion process will be discussed, followed by their applications in different areas, especially in the biological field for biosensing.
Tripeptidase gene (pepT) of Lactococcus lactis: molecular cloning and nucleotide sequencing of pepT and construction of a chromosomal deletion mutant (1994)

The gene encoding a tripeptidase (pepT) of Lactococcus lactis subsp. cremoris (formerly subsp. lactis) MG1363 was cloned from a genomic library in pUC19 and subsequently sequenced. The tripeptidase of L. lactis was shown to be homologous to PepT of Salmonella typhimurium with 47.4% identity in the deduced amino acid sequences. L. lactis PepT was enzymatically active in Escherichia coli and allowed growth of a peptidase-negative leucine-auxotrophic E. coli strain by liberation of Leu from a tripeptide. Using a two-step integration-excision system, a pepT-negative mutant of L. lactis was constructed. No differences between the growth of the mutant and that of the wild-type strain in milk or in chemically defined medium with casein as the sole source of essential amino acids were observed.
El impacto de los trabajos forzados en la economía vasconavarra (1937-1945) (2012)

With this paper we try to make public some data and hypotheses on the impact of forced labour in the Spanish economy within the context of the Civil War and immediate post-war, so that we can bring Spanish historiography closer to some of the central debates of European research about forced labour in a war and dictatorship context, such as the weight of forced labour in a war and post-war economy, and the strategies of the State and enterprises towards it.
Response of Morphogenesis and Cell Proliferation to Allelopatic Compounds on Rice Germplasm (2023)

Allelopathic compounds are chemical substances produced by weeds, including Imperata cylindrica, that can inhibit the absorption of nutrients and cell growth of neighboring plants. Tissue culture technique can be used to determine the effect of media composition on morphogenesis and cell proliferation, as indicated by related genes. Through the process of somatic embryogenesis, cells regenerate into whole plants via several phases initiated by gene expression. Genes are the signal that connects environmental cues and plant cells. The objective of this study was to determine the effect of allelopathic compounds from Imperata cylindrica root extract on the morphogenesis and cell proliferation of indigenous Indonesian rice plant. The used callus was derived from the seeds of Bondoyudo, Caok, Ciliwung and Situbagendit rice varieties. The results of callus induction were selected and transferred to regeneration media that had been treated with I. cylindrica roots extract of 2.5 g/L and 5 g/L. The development of callus was monitored weekly for six weeks. As for the molecular analysis, four-week-old callus was utilized. The results demonstrated that the root extract of Imperata cylindrica had a specific effect on the morphogenesis and proliferation of rice cells depending on concentration and target plant. At a molecular level, the expression of the OsBBM, OsLEA, OsLEC1, OsSERK, and OsWOX4 genes was affected differently by the administration of allelopathic compounds at a concentration of 5 g/L on a molecular level.
Stepwise Morphological and Functional Maturation of Mechanotransduction in Rat Outer Hair Cells (2007)

Inner ear mechanosensory hair cells convert mechanical vibrations into electrical signals via the coordinated interaction of multiple proteins precisely positioned within the sensory hair bundle. Present work identifies the time course for the acquisition and maturation of mechanoelectric transduction (MET) in rat cochlea outer hair cells maintained in organotypic cultures. A spatiotemporal developmental progression was observed morphologically and functionally with basal cochlea maturation preceding apical cochlea by 2-3 d in all measured properties. The fraction of mechanosensitive cells increased rapidly, with a midpoint at postnatal day 0 for basal cells, and correlated with myosin IIIa immunoreactivity. MET current magnitude increased over several days. Adaptation lagged the onset of transduction by a day and matured more slowly, overlapping but preceding the rise in myosin Ic immunoreactivity. Less than approximately 25% of myosin Ic expression was required for the mature adaptation response, suggesting multiple roles for this protein in hair bundle function. Directional sensitivity, lacking in immature responses, developed rapidly and correlated with the pruning of radial links and an increase in tenting of stereociliary tips. Morphological and electrophysiological data support a hypothesis in which key elements arrive independently at the site of MET, with a mature response occurring as membrane tension increases, likely by the increased tensioning of the tip link with the onset of adaptation. Organotypic cultures developed normal, tonotopically specific, MET response properties, suggesting that maturation was not influenced significantly by external factors such as innervation, endolymph, normal mechanical stimulation, or an intact organ of Corti.
Self‐help in housing and chengzhongcun in China's urbanization (2003)

This article focuses on the housing issues of rural migrants arising from urbanization, with particular reference to chengzhongcun , a topic with considerable impact on policymaking. An attempt is made to understand the underlying rationale of self‐help in housing and the important role of chengzhongcun in sheltering rural migrants in the context of China's rural‐urban dichotomy. As demonstrated in this study, chengzhongcun accommodate, with little in the way of government resources and assistance, millions of rural migrants because of their social accessibility and affordability. While not denying their social problems, we argue that chengzhongcun in fact act as an innovative and positive agent to promote urbanization in present day China by housing massive numbers of rural migrants and assimilating them into cities. Current government policies towards chengzhongcun have generated a wide range of interest conflicts and confrontations. The consequences of such conflicts show that the government policies were problematic and unworkable, as they violated basic market principles as well as citizen rights. Policy strategy towards the redevelopment of chengzhongcun must acknowledge their credibility in the Chinese road to urbanization and requires more thoughtful and prudent consideration of migrants' demands for affordable housing.
The molecular biology of caliciviruses. (1997)

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher and we support and invest in the microbiology community, to the benefit of everyone. This supports our principal goal to develop, expand and strengthen the networks available to our members so that they can generate new knowledge about microbes and ensure that it is shared with other communities.
On the computational complexity of bisimulation (1995)

article Free Access Share on On the computational complexity of bisimulation Authors: Faron Moller SICS, Kista, Sweden SICS, Kista, SwedenView Profile , Scott A. Smolka State Univ. of New York at Stony Brook, Stony Brook State Univ. of New York at Stony Brook, Stony BrookView Profile Authors Info & Claims ACM Computing SurveysVolume 27Issue 2pp 287–289https://doi.org/10.1145/210376.210406Published:01 June 1995Publication History 6citation492DownloadsMetricsTotal Citations6Total Downloads492Last 12 Months28Last 6 weeks9 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Changes in the Enzymes for Fatty Acid Synthesis and Desaturation during Acclimation of Developing Soybean Seeds to Altered Growth Temperature (1989)

Temperature-induced changes in the enzymes for fatty acid synthesis and desaturation were studied in developing soybean seeds (Glycine max L. var Williams 82). Changes were induced by culture of the seed pods for 20 hours in liquid media at 20, 25, or 35 degrees C. Linoleoyl and oleoyl desaturases were 94 and 10 times as active, respectively, in seeds cultured at 20 degrees C as those cultured at 25 degrees C. Both desaturases had negligible activity in seeds cultured at 35 degrees C compared to seeds cultured at 20 degrees C. Though less dramatic, other enzymes also showed differences in activity after 20 hours in culture at 20, 25, or 35 degrees C. Stearoyl-acyl carrier protein (ACP) desaturase and CDP-choline:diacylglycerol phosphorylcholine transferase were most active in preparations from 20 degrees C cultures. Activities were twofold lower at 25 degrees C and a further threefold lower in 35 degrees C cultures. Cultures from 25 and 35 degrees C had 60 and 40%, respectively, of the phosphorylcholine:CTP cytidylyl transferase activity present in cultures grown at 20 degrees C. Fatty acid synthetase, malonyl-coenzyme A:ACP transacylase, palmitoyl-ACP elongation, and choline kinase were not significantly altered by culture temperature. These data suggest that the enzymes for fatty acid desaturation and phosphatidylcholine synthesis can be rapidly modulated in response to altered growth temperatures, while the enzymes for fatty acid synthesis and elongation are not.
Mammalian microRNA: an important modulator of host-pathogen interactions in human viral infections (2016)

MicroRNAs (miRNAs), which are small non-coding RNAs expressed by almost all metazoans, have key roles in the regulation of cell differentiation, organism development and gene expression. Thousands of miRNAs regulating approximately 60 % of the total human genome have been identified. They regulate genetic expression either by direct cleavage or by translational repression of the target mRNAs recognized through partial complementary base pairing. The active and functional unit of miRNA is its complex with Argonaute proteins known as the microRNA-induced silencing complex (miRISC). De-regulated miRNA expression in the human cell may contribute to a diverse group of disorders including cancer, cardiovascular dysfunctions, liver damage, immunological dysfunction, metabolic syndromes and pathogenic infections. Current day studies have revealed that miRNAs are indeed a pivotal component of host-pathogen interactions and host immune responses toward microorganisms. miRNA is emerging as a tool for genetic study, therapeutic development and diagnosis for human pathogenic infections caused by viruses, bacteria, parasites and fungi. Many pathogens can exploit the host miRNA system for their own benefit such as surviving inside the host cell, replication, pathogenesis and bypassing some host immune barriers, while some express pathogen-encoded miRNA inside the host contributing to their replication, survival and/or latency. In this review, we discuss the role and significance of miRNA in relation to some pathogenic viruses.
Vitamin D binding protein sequesters monomeric actin in the circulation of the rat. (1987)

Plasma vitamin D binding protein (DBP) may scavenge actin released during cell lysis. We examined the plasma disappearance and tissue appearance of 125I-DBP, 125I-G-actin, and the DBP-G-actin complex after their intravenous administration to rats. The plasma disappearance of DBP and DBP-actin were indistinguishable, with rapid initial (t1/2 = 2.6 h) and slower second (t1/2 = 7 h) slopes. After 125I-G-actin (nanomole) injection, plasma disappearance paralleled that of DBP and DBP-actin. All injected actin was associated with DBP, without evidence of free actin, actin-gelsolin complexes or actin oligomers. Tissue appearances of 125I-apo-DBP (apo) or holo-DBP were similar, with highest accumulations in perfused liver, kidney, and skeletal muscle. Although more complex phenomena (plasma entry of F-actin and intracellular actin binding proteins) would occur in vivo after cell lysis, our results suggest a role for DBP in the sequestration and disposition of actin monomers in the circulation.