gebhart/patrepeval huggingface dataset:
More than just water channels: unexpected cellular roles of aquaporins (2005)

Aquaporins (AQPs) are membrane proteins that transport water and, in some cases, also small solutes such as glycerol. AQPs are expressed in many fluid-transporting tissues, such as kidney tubules and glandular epithelia, as well as in non-fluid-transporting tissues, such as epidermis, adipose tissue and astroglia. Their classical role in facilitating trans-epithelial fluid transport is well understood, as in the urinary concentrating mechanism and gland fluid secretion. AQPs are also involved in swelling of tissues under stress, as in the injured cornea and the brain in stroke, tumor and infection. Recent analysis of AQP-knockout mice has revealed unexpected cellular roles of AQPs. AQPs facilitate cell migration, as manifested by reduced tumor angiogenesis in AQP1-knockout mice, by a mechanism that might involve facilitated water transport in lamellipodia of migrating cells. AQPs that transport both glycerol and water regulate glycerol content in epidermis and fat, and consequently skin hydration/biosynthesis and fat metabolism. AQPs might also be involved in neural signal transduction, cell volume regulation and organellar physiology. The many roles of AQPs could be exploited for clinical benefit; for example, treatments that modulate AQP expression/function could be used as diuretics, and in the treatment of brain swelling, glaucoma, epilepsy, obesity and cancer.
MINORIAS NO SÉCULO XXI, UMA ANÁLISE SOBRE OS DIREITOS DOS TRANSEXUAIS EM TERRITÓRIO BRASILEIRO (2014)

O presente trabalho tem como proposito uma analise dos direitos dos transexuais dentro da perspectiva juridica, psicologica e medica dentro do territorio brasileiro, ate entao, pouco divulgado em cenario academico, mas com a recente evolucao das medidas protetivas e de novas legislacoes internas, alem de modificacoes de legislacoes que o Brasil ja possuia, despertou o interesse de se fazer estudar. O foco, portanto, esta no aspecto juridico, apontando a legislacao que a tutela, apresentando tambem normas que nao estao diretamente ligadas a defesa dos transexuais, mas que servem e sao utilizadas para se fazer proteger os direitos fundamentais desse grupo tao descriminado. Primeiramente antes de adentrar no contexto juridico contemporâneo, o autor inicia analisando e diferenciando as novas sexualidades em um aspecto geral, passando pela tutela juridica penal e civil dentro do territorio brasileiro apresentando decisoes sobre o fato no solo patrio, entrando tambem nas resolucoes nao propriamente juridica do Conselho Federal de Medicina (CFM), culminando na analise das consequencias psicologicas antes e depois da cirurgia.
RPM: a rapid prototyping engine for multiprocessor systems (1995)

RPM enables rapid prototyping of different multiprocessor architectures. It uses hardware emulation for reliable design verification and performance evaluation. The major objective of the RPM project is to develop a common, configurable hardware platform to accurately emulate different MIMD systems with up to eight execution processors. Because emulation is orders of magnitude faster than simulation, an emulator can run problems with large data sets more representative of the workloads for which the target machine is designed. Because an emulation is closer to the target implementation than an abstracted simulation, it can accomplish more reliable performance evaluation and design verification. Finally, an emulator is a real computer with its own I/O; the code running on the emulator is not instrumented. As a result, the emulator looks exactly like the target machine (to the programmer) and can run several different workloads, including code from production compilers, operating systems, databases, and software utilities.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A novel deletion in the RET proto-oncogene found in sporadic medullary thyroid carcinoma. (1996)

Germ line point mutations in the RET proto-oncogene have been implicated in four inherited disorders: multiple endocrine neoplasia 2A (MEN 2A) and 2B (MEN 2B); familial medullary thyroid carcinoma (FMTC); and Hirschprung's disease, a congenital lack of enteric plexus neurons. Oncogenically activated RET has also been demonstrated in some sporadic medullary thyroid tumors, which show somatic missense mutations in the same regions as those found in MEN 2B. Upon screening archival sporadic MTC tumor tissue by nonradioactive single-strand conformational polymorphism analysis (SSCP), a markedly divergent exon 11 pattern was found in an unusually aggressive neoplasm. Sequencing of PCR amplified DNA revealed the deletion of nine bases encompassing a key cysteine codon at position 1831-3, often altered in MEN 2A. Normal thyroid tissue from the same patient showed a normal SSCP pattern and sequence for this exon. This novel somatic mutation further implicates the RET proto-oncogene in the development of MTC.
Serum miR‐19a expression correlates with worse prognosis of patients with non‐small cell lung cancer (2013)

High serum miR-19a expression may be an independent poor prognostic factor for survival in NSCLC patients.
Yttrium Hydrido Complexes that Contain a Less “Constrained Geometry” Ligand:  Synthesis, Structure, and Efficient Hydrosilylation Catalysis (2001)

σ-Bond metathesis of the new linked amino-cyclopentadiene (C5Me4H)CH2SiMe2NHCMe3 with Y(CH2SiMe3)3(THF)2 gave the alkyl-yttrium complex [Y(η5:η1-C5Me4CH2SiMe2NCMe3)(CH2SiMe3)(THF)] (1) in 66% yield. Compound 1 was characterized by NMR spectroscopy and X-ray crystal structure analysis. By hydrogenolysis, 1 was quantitatively converted into the dimeric hydride [Y(η5:η1-C5Me4CH2SiMe2NCMe3)(THF)(μ-H)]2 (2). X-ray crystal structure analysis revealed a Y2H2 core connected to two [Y(η5:η1-C5Me4CH2SiMe2NCMe3)(THF)] fragments in a skewed manner, with a relatively long yttrium−yttrium distance of 3.7085(8) Å. In solution, two diastereomers were observed by 1H NMR spectroscopy at −78 °C. Dimer 2 was shown to efficiently catalyze the hydrosilylation of 1-decene with PhSiH3 to give the terminal silane nC10H21SiH2Ph exclusively. Although the product of addition of the yttrium-hydride complex 2 to styrene, [Y(η5:η1-C5Me4CH2SiMe2NCMe3){CH(CH3)Ph}(THF)] (3), contains a 1-phenethyl ligand derived from internal addition, hydrosilylation of styrene with PhSiH3 catalyzed by 2 gave both terminal- and internal-hydrosilylated products in a ratio of 1:1.4.
Specific correction of a splice defect in brain by nutritional supplementation (2011)

Recent studies emphasize the importance of mRNA splicing in human genetic disease, as 20-30% of all disease-causing mutations are predicted to result in mRNA splicing defects. The plasticity of the mRNA splicing reaction has made these mutations attractive candidates for the development of therapeutics. Familial dysautonomia (FD) is a severe neurodegenerative disorder, and all patients have an intronic IVS20+6T>C splice site mutation in the IKBKAP gene, which results in tissue-specific skipping of exon 20 and a corresponding reduction in ikappaB kinase complex associated protein (IKAP) levels. We created transgenic mouse lines using a human IKBKAP bacterial artificial chromosome (BAC) into which we inserted the IKBKAP splice mutation (FD BAC) and have shown that the transgenic mice exhibit the same tissue-specific aberrant splicing patterns as seen in FD patients. We have previously demonstrated that the plant cytokinin kinetin can significantly improve the production of wild-type IKBKAP transcripts in FD lymphoblast cell lines by improving exon inclusion. In this study, we tested the ability of kinetin to alter IKBKAP splicing in the transgenic mice carrying the FD BAC and show that it corrects IKBKAP splicing in all major tissues assayed, including the brain. The amount of wild-type IKBKAP mRNA and IKAP protein was significantly higher in the kinetin-treated mice. These exciting results prove that treatment of FD, as well as other mechanistically related splicing disorders, with kinetin holds great promise as a potential therapeutic aimed at increasing normal protein levels, which may, in turn, slow disease progression.
A comparative study of women with chronic pelvic pain, chronic nonpelvic pain and those with no history of pain attending general practitioners (1998)

These findings indicate that women with chronic pelvic pain have a higher incidence of past sexual abuse compared with women in a comparison pain group and with women with no pain.
Two Novel Ubiquitin-fold Modifier 1 (Ufm1)-specific Proteases, UfSP1 and UfSP2 (2006)

Ubiquitin-fold modifier 1 (Ufm1) is a recently identified new ubiquitin-like protein, whose tertiary structure displays a striking resemblance to ubiquitin. Similar to ubiquitin, it has a Gly residue conserved across species at the C-terminal region with extensions of various amino acid sequences that need to be processed in vivo prior to conjugation to target proteins. Here we report the isolation, cloning, and characterization of two novel mouse Ufm1-specific proteases, named UfSP1 and UfSP2. UfSP1 and UfSP2 are composed of 217 and 461 amino acids, respectively, and they have no sequence homology with previously known proteases. UfSP2 is present in most, if not all, of multicellular organisms including plant, nematode, fly, and mammal, whereas UfSP1 could not be found in plant and nematode upon data base search. UfSP1 and UfSP2 cleaved the C-terminal extension of Ufm1 but not that of ubiquitin or other ubiquitin-like proteins, such as SUMO-1 and ISG15. Both were also capable of releasing Ufm1 from Ufm1-conjugated cellular proteins. They were sensitive to inhibition by sulfhydryl-blocking agents, such as N-ethylmaleimide, and their active site Cys could be labeled with Ufm1-vinylmethylester. Moreover, replacement of the conserved Cys residue by Ser resulted in a complete loss of the UfSP1 and UfSP2 activities. These results indicate that UfSP1 and UfSP2 are novel thiol proteases that specifically process the C terminus of Ufm1.
Existence And Global Exponential Stability Of Periodic Solutions Of Cellular Neural Networks With Distributed Delays And Impulses On Time Scales (2011)

In this paper, by using Mawhin-s continuation theorem of coincidence degree and a method based on delay differential inequality, some sufficient conditions are obtained for the existence and global exponential stability of periodic solutions of cellular neural networks with distributed delays and impulses on time scales. The results of this paper generalized previously known results.
Diagnostic value of multiplex real-time polymerase chain reaction for mycoplasma and trachomatis infection in respiratory tract infection children (2017)

Objective
To investigate the diagnostic value of multiplex real-time PCR (TaqMan probe) for simultaneous detection of Mycoplasma pneumoniae (MP) , Chlamydophila pneumonia (CP) and Chlamydia trachomatis (CT) in hospitalized children with respiratory tract infection.


Methods
The standard curves were plotted based on recombinant plasmids that was amplified after gradient dilution, so as to measure the amplification efficiency of the multiplx real-time PCR method, and the sensitivity, specificity and reproducibility were verified. There were 151 throat swab samples from hospitalized children with suspected respiratory tract infection collected. The MP, CP and CT gene fragments were detected by multiplex real-time PCR. There were 128 samples identified by MP-IgM and CP-IgM serological detection to evaluate the diagnostic value in clinical detection.


Results
The amplification efficiency of MP, CP and CT primers and probes reached up to 109.20%, 116.90% and 112.80%; and the minimum detectable concentration were 50, 25 and 25 pg/μL, respectively. No cross reactions with other known pathogens was observed, and coefficients of variation among different amplification reactions were all less than 3%. In 151 samples, the positive rates of MP, CP and CT by multiplex real-time PCR were 23.84% (36 samples) , 17.88% (27 samples) and 2.65% (4 samples) . In 128 samples, the positive rates of MP-IgM and CP-IgM were 22.65% and 14.06%, respectively.


Conclusions
The multiplex real-time PCR method can provide rapid and reliable evidence for early pathogen detection in children with respiratory tract infection.


Key words: 
Polymerase chain reaction; Respiratory tract infection; Mycoplasma pneumonia; Chlamydophila pneumonia; Chlamydia trachomatis
Identification of SopE2, a <i>Salmonella</i> Secreted Protein Which Is Highly Homologous to SopE and Involved in Bacterial Invasion of Epithelial Cells (2000)

Type III secreted Sop protein effectors are delivered into target eukaryotic cells and elicit cellular responses underlying Salmonella pathogenicity. In this work, we have identified another secreted protein, SopE2, and showed that SopE2 is an important invasion-associated effector. SopE2 is encoded by the sopE2 gene which is present and conserved in pathogenic strains of Salmonella. SopE2 is highly homologous to SopE, a protein encoded by a gene within a temperate bacteriophage and present in only some pathogenic strains.
Leviticus. By J. R. Porter. (The Cambridge Bible Commentary on the N.E.B.) Cambridge University Press, 1976. Pp. x + 232. £5·95 (cloth), £2·95 (paper). (1978)

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Scalable synthesis of self-assembling nanoparticle clusters based on controlled steric interactions (2011)

A simple one-pot method is presented for the synthesis of amphiphilic gold nanoparticles that can spontaneously self-assemble into stable clusters with controllable geometry. The technique is based on the control of colloidal interactions through sequential functionalization of the surfaces with long polyethylene glycol (PEG) chains followed by short alkane–thiol molecules. This process renders the gold nanoparticles amphiphilic and initiates the self-assembly of stable clusters in the dispersion. It is shown that the dominant structures and sizes of the clusters (i.e. singlets, doublets, triplets and others) are directly and reproducibly controlled by the concentration of PEG chains that are linked to the particle surface. Changes in the structure of the clusters are systematically characterized by small angle X-ray scattering (SAXS), dynamic light scattering (DLS) and transmission electron microscopy (TEM). Systematic shifts in the plasmon resonance peak of the gold nanoparticle clusters demonstrate that this new method for self-assembly can be effectively used to tune optical properties with great precision. With this new approach, we show that it is possible to fabricate large numbers of amphiphilic nanoparticles and clusters with controllable structure by manipulating the repulsive steric forces that are imparted by surface bound polymers and the attractive interactions of the hydrophobic alkane–thiols. The major advantage of this technique lies in its simplicity, versatility and potential for scalable production of self-assembled ‘colloidal molecules’.
Indirect photometric chromatography of anions using sodium naphthalenetrisulfonate (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIndirect photometric chromatography of anions using sodium naphthalenetrisulfonateShahbaz A. Maki and Neil D. DanielsonCite this: Anal. Chem. 1991, 63, 7, 699–703Publication Date (Print):April 1, 1991Publication History Published online1 May 2002Published inissue 1 April 1991https://doi.org/10.1021/ac00007a010RIGHTS & PERMISSIONSArticle Views78Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (552 KB) Get e-Alertsclose Get e-Alerts
Emerging cancer incidence trends in Canada: The growing burden of young adult cancers (2020)

Incidence among young adults is increasing for some cancers associated with obesity but decreasing for many cancers associated with infections or smoking. Although further studies are needed to replicate these findings and understand the etiology of early-onset cancers, measures to promote healthy behaviors in young adults warranted.
On the Impossibility of Cryptography Alone for Privacy-Preserving Cloud Computing. (2010)

All in-text\treferences\tunderlined\tin\tblue\tare\tlinked\tto\tpublications\ton\tResearchGate, letting you\taccess\tand\tread\tthem\timmediately.
‘Artilysation’ of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci (2016)

Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10 min. In addition, lower doses of Art-240 are required to achieve the same bactericidal effect.
RMB-a reconfigurable multiple bus network (2002)

The heart of a massively parallel computer is its interconnection network. In this article we present a reconfigurable multiple bas network to support circuit switching as means of communication between processors of a multiprocessor machine. The main contribution of the papers is in demonstrating the simplicity of the routing hardware whilst still providing modularity and full utilization of the multiple bus system. A comparison with major interconnection network is also presented.
Zeolitic imidazolate framework composite membranes and thin films: synthesis and applications (2014)

Zeolitic imidazolate frameworks (ZIFs), a subclass of metal organic frameworks, are built of tetrahedral metal ions bridged by imidazolates. They have permanent porosity and relatively high thermal and chemical stability, which make them attractive candidates for many industrial applications. In recent years, significant progress has been made in developing ZIFs into membranes and thin films for gas separation, liquid separation (pervaporation) and functional devices. Various techniques, such as direct synthesis, secondary synthesis, reactive seeding and functional chemicals as linkers, and contra-diffusion synthesis, have been reported for the fabrication of ZIF membranes and films. As ZIFs have good compatibility with polymers, they have been incorporated into polymers with high loadings to form mixed matrix membranes. The resulting symmetric dense or asymmetric composite membranes exhibit good performance in gas separation and liquid separation via pervaporation. The recent developments of ZIF membranes/films, ZIF-polymer mixed matrix membranes and their applications are reviewed in this article.
(Invited) Integration Challenges for Copper Damascene Electroplating (2010)

Copper electroplating has become the commonly used technology to manufacture interconnects in semiconductor products. The art of copper damascene electroplating is to provide a void-free fill for all design features, but to avoid high topography on top of those structures at the same time. The desired direction is superfill. The most widely published way to control this is to find the right electrolyte additive type and concentration for the features that have to be filled. However, there is a variety structures on a semiconductor chip and in a high-volume manufacturing there is a variety of products that may have different optimal fill conditions. In this paper, fill and topography control by current density modification and its implication to CMP will be shown.
An object-oriented specification and its generation for compiler (1992)

In this paper, an object-oriented approach to compiler specification is presented. The syntax symbols are treated as templates which instantiate nodes of parse trees to perform semantics analysis. The compiler generating techniques, specifically for this approach, are also presented in brief.
DPP‐4 Resistant Glucagon‐Like Peptide‐1 Analog LY548806: A Novel Agent for Control of Acute Hyperglycemia (2007)

The present studies identified a novel DPP‐4 resistant GLP‐1 analogue LY548806 and characterized its pharmacological activity in a rat model of acute hyperglycemia. LY548806 was more than 20‐fold less susceptible to DPP‐4 cleavage and exhibited a 50‐fold greater physical stability over native GLP‐1, and was a full agonist with an EC 50 of 10.6 ± 1.6 pM in vitro . In an acute hyperglycemia model, continuous infusion of LY548806 (0.1–10 μg/kg/hr) or insulin (0.01 U–0.1U/kg/hr) via the right jugular vein resulted in dose‐dependent reduction of glucose levels with estimated ED 50 values of 0.32 ± 0.11 ug and 0.08 ± 0.00 U respectively. In contrast to insulin, LY548806 did not produce hypoglycemia at higher dose levels. Continuous infusion of LY548806 (10 μg/kg/hr) or insulin (0.1 U/kg/hr) did not affect the plasma levels of the endogenous glucoregulatory hormones corticosterone, growth hormone, catecholamines, or insulin, indicating an insulin‐independent effect of LY548806 in this model. LY548806 significantly lowered the plasma levels of free fatty acids (FFA) and glucagon, whereas insulin only reduced FFA levels. In conclusion, we have identified a stable GLP‐1 analogue with improved solubility which effectively ameliorates acute hyperglycemia without the risk of hypoglycemia and may represent a novel therapeutic strategy for acute critical illness associated with acute hyperglycemia.