gebhart/patrepeval huggingface dataset:
Shape of large bound polysomes in cultured fibroblasts and thyroid epithelial cells (1999)

Large bound polysomes were observed by conventional electron microscopy in surface or en face views of the rough endoplasmic reticulum (RER) in two cultured cell types. Cultured thyroid follicular epithelial cells and dermal fibroblasts, both from rats, were prepared for electron microscopy. Ultrathin sections were cut in the plane of the flattened cells to maximize the incidence of RER surface views. Some observations were also made on tissue sections of rat thyroid. Most of the large, RER-bound polysomes in both cell types appeared as two parallel rows of ribosome, thus resembling the shape of long hairpins, although probably closed at both ends. The two parallel rows of ribosomes were about 14 nm apart, and the center-to-center distance between ribosomes in the strands averaged 25 nm. Most of the large bound polysomes in thyroid epithelial cells were presumably making thyroglobulin subunits (330 kDa), while a majority of those in the fibroblasts were probably making prepro-alpha chains of collagen I (150 kDa). It was not possible in this material to see complete large polysomes, because their size usually caused them to extend out of the plane of section. In addition to the hairpin polysomes, there were smaller numbers of other forms. A characteristic large spiral polysome was seen occasionally in both cell types and contained as many as 31 ribosomes. One or two dense particles were sometimes seen in the center of spiral or circular polysomes. The consistent hairpin shape of most large bound polysomes observed in this study suggests that their shape is quite stable.
Synthesis, anti‐HIV and Antitubercular Activities of Lamivudine Prodrugs. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
SPLUNC1 (PLUNC) is expressed in glandular tissues of the respiratory tract and in lung tumours with a glandular phenotype (2005)

Short PLUNC1 (SPLUNC1) is the founding member of a novel family of proteins (PLUNC) expressed in the upper respiratory tract that may function in host defence. It is one of the most highly expressed genes in the upper airways and the protein has been detected in sputum and nasal secretions. This study describes, for the first time, the precise cellular localization of SPLUNC1 in human tissues from the respiratory tract. Although SPLUNC1 is found in some epithelial cells of the upper airways and coats the surface epithelial cell lining of the major airways, the most significant site of protein localization is in mucous cells and ducts of submucosal glands. Intense staining is also seen in minor glands of the nose, sinuses, posterior tongue and tonsil, suggesting that the protein is secreted into mucoid secretions of these tissues, where it probably functions in host defence. No staining was seen in peripheral lung tissue. As SPLUNC1 has been suggested to be a novel lung cancer marker, a limited panel of lung cancers was also studied. The findings suggest that SPLUNC1 is commonly expressed in adenocarcinomas, muco-epidermoid carcinoma, and bronchio-alveloar carcinoma, and is absent from small-cell carcinoma and squamous cell carcinoma. This expression pattern is consistent with the presumed phenotypic origin of these tumours and suggests that SPLUNC1 may be a useful marker for lung cancer.
Expression of APRG1 mRNA in breast cancer tissues (2007)

Objective To detect the expression of APRG1 mRNA in breast cancer,cancer-adjacent tissues and normal breast tissues from the patients with benign breast diseases,and study the relationship between APRG1 mRNA expression in breast cancer tissues with different clinical pathological characteristics.Methods Seventy-eight cancer tissues,cancer-adjacent tissues and 32 normal breast tissues were analyzed.The level of transcription of APRG1 was determined by reverse transcriptase polymerase chain reaction(RT-PCR).Results The level of APRG1 mRNA expression was lower in malignant tissues than that in normal breast tissues.The expression fell further with increasing TNM stage.This became statistically significant when TNM stage Ⅲ compared to stage Ⅰ(P0.05).The reduction of APRG1 mRNA expression was lower in nuclear grade 3 tumors than that in grade 1(P0.05).Conclusion This study shows a down-regulating trend in APRG1 mRNA expression in malignant tissues,especially in more advanced disease,suggesting that APRG1 is a tumor suppressor gene.
Field performance of embryogenic cell suspension-derived banana plants (Musa 'AAA', cv. 'Grand Nain') (1999)

Growth and yield characteristics of two different clones of banana plants (Musa AAA cv. Grand Nain) originated from embryogenic cell suspensions were studied. These characteristics were compared with those plants produced by the conventional in vitro budding multiplication method. Two types of variants were observed during the acclimatization phase among 500 embryogenic cell suspension derived plants. The first type concerned banana plants with "variegated or deformed limbs", the second type concerned plants with "double limb". During the field growth, these two variant types produced plants not morphologically different from the other plants. A Fisher block model was used to compare the field performances of the two clones produced through the two in vitro propagation techniques. The analysis of variance showed that there were no significant differences between the plants produced by either micropropagation techniques for the plant height and circumference, the length of the reference limb, the number of inflorescences and fruits, the bunch weight, the period of time between planting and flowering, and between planting and harvesting. This study showed that banana plants with an agronomical behaviour similar to those produced by the conventional in vitro budding method could be regenerate from embryogenic cell suspension. Embryogenic cell suspensions can be considered as an adequate tool for genetic transformation in banana genetic improvement programs. The study also support the idea according to which somatic embryogenesis could constitute a new propagation technique for bananas. However, field experiments on a larger scale are now essential to confirm this last hypothesis
High fibrinogen and plasminogen activator inhibitor activity in growth hormone-deficient adults. (1994)

Hypopituitary patients on routine replacement therapy except growth hormone (GH) have an increased risk of death from cardiovascular diseases compared with healthy subjects. Untreated GH deficiency might explain the premature death from vascular disease. Plasminogen activator inhibitor (PAI-1) activity, fibrinogen, insulin, blood lipid, and blood pressure levels were studied in 20 GH-deficient adults (10 men, 10 women) 50 +/- 11 years old with routine hormone replacement therapy (except GH) and compared with 20 healthy control subjects matched for sex, age, and body mass index. GH-deficient subjects had a higher waist-to-hip circumference ratio (P < .001), serum triglycerides (P < .02), PAI-1 activity (13.2 +/- 10.6 versus 6.8 +/- 4.8 U/mL [P < .05]), and fibrinogen (3.2 +/- 0.7 versus 2.4 +/- 0.6 g/L [P < .001]) and lower blood glucose (P < .05) compared with control subjects. Blood pressure, insulin, and cholesterol levels were similar. The aberrations found in this study might contribute to an increased atherothrombotic propensity and play a role in the pathogenesis of cardiovascular disease.
A novel ferrocenyl diselenide for the catalytic asymmetric aryl transfer to aldehydes (2001)

An oxazolinyl ferrocenyl diselenide was synthesised by directed ortho-metalation and used as catalyst precursor in the asymmetric addition of diethyl- and diphenylzinc to various aldehydes, yielding synthetically useful secondary alcohols, of which some are difficult to access using other catalytic methodologies. Enantioselectivities of up to 44% for the former and up to 85% for the latter transformations were obtained.
Vehicle Ego-Localization in Autonomous Lane-Keeping Evasive Maneuvers (2016)

This paper presents a method for precise and reliable vehicle ego-localization required for autonomous vehicle guidance in highly dynamic lane-keeping evasive maneuvers. It combines the measurements of a low-cost mono-camera used for lane detection with the information of standard vehicle sensors. The proposed data-fusion concept uses an advanced vehicle model based Extended Kalman Filter that takes into account the nonlinear tire characteristics at the limits of driving physics. Extensions of the fusion concept are derived which compensate the application-related limitations of the camera. The experimental results of a pedestrian collision avoidance maneuver demonstrate the accuracy and robustness of the method.
New Chemical Synthesis of Functionalized Arylzinc Compounds from Aromatic or Thienyl Bromides under Mild Conditions Using a Simple Cobalt Catalyst and Zinc Dust (2003)

A new chemical method for the preparation of arylzinc intermediates is described in acetonitrile, on the basis of the activation of aryl bromides by low-valent cobalt species arising from the reduction of cobalt halide by zinc dust. This procedure allows for the synthesis of a variety of functionalized aryl- and thienylzinc species in good to excellent yields. The versatility and the simplicity of that original method represent an alternative to most known procedures.
Beginning J2me: From Novice To Professional (Beginning from Novice to Professional) (2005)

Testis gene Znf230 may play a role in mammalian spermatogenesis according to previous reports. Deleting 5' important exons to block the formation of protein was a routine way in gene-knockout experiments. To investigate the physiological function of Znf230 gene, the mutant mice with disrupted exon 2 of Znf230 were generated in this study. Results showed that, mutant Znf230 mice were fertile and showed normal body, genitourinary organs, testes weights, and spermatid number but the litter size of the offspring reduced with unclear reasons. Hematoxylin and eosin staining showed that the testicular tissue of mutant mice was intact. Reverse transcriptase polymerase chain reaction analysis showed that two novel mutant transcripts appeared in the mutant mice: the short one including exon-1 and exon-3 to -6, the long one unexpectedly containing a partial sequence from the pPNT vector acting as a new exon 2. Bioinformatic analysis of the long transcript revealed that it might code a 24-kDa N-terminal mutant protein with the same 182 amino acids as that of the wild-type Znf230 in the C-terminus, indicating that the potential functional region of C3HC4-type RING finger was intact in mutant protein. Western blot and immunohistochemistry analyses also implied that this N-terminal mutation of Znf230 might not disrupt the possible role that wild-type Znf230 played in spermatogenesis. In summary, a potential exon structure in the targeting vector sequence involved in the expression of targeting Znf230 gene and disturbed the strategy of this gene-targeting experiment.
Delivery of dsRNA with lactic acid bacteria for RNA interference (2007)

RNA interference (RNAi) mechanism is promising for gene-targeted therapy. In this study, Lactobacillus casei were engineered to produce long double-stranded RNA (dsRNA) or short hairpin RNA (shRNA) for RNAi-delivery to the intestine. We prepared two kinds of L. casei; one produces shRNA which works in mammalian cells, and the other produces long dsRNA which works in nematode. We confirmed the expression of shRNA and dsRNA in the transformed L. casei by Northern blotting and RT-PCR analyses.
Labor in the New Deal (1933)

Review| November 01 1933 Labor in the New Deal E. Francis Brown E. Francis Brown Search for other works by this author on: This Site PubMed Google Scholar Current History (1933) 39 (2): 203–210. https://doi.org/10.1525/curh.1933.39.2.203 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation E. Francis Brown; Labor in the New Deal. Current History 1 November 1933; 39 (2): 203–210. doi: https://doi.org/10.1525/curh.1933.39.2.203 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentCurrent History Search This content is only available via PDF. © 1933 by The Regents of the University of California1933 Article PDF first page preview Close Modal You do not currently have access to this content.
Abstract 1689: Synergistic effect of JQ1 and rapamycin for treatment of human osteosarcoma (2014)

Abstract Bromodomain and extra terminal domain (BET) proteins are important epigenetic regulators facillitating the transcription of genes in chromatin areas linked to acetylated histones. JQ1, a BET protein inhibitor, has antiproliferative activity againts many hematological and solid cancers, mainly through inhibition of c-myc and upregulation of p21. In this research, we investigated the use of JQ1 for human osteosarcoma (OS) treatment, due to the frequent copy number gain and overexpression of c-myc in OS cells. JQ1 significantly inhibited the proliferation and survival of seven OS cells (U2OS, G292, MG-63, HT-161, MNNG/HOS, SAOS-2, SJSA), at a relatively higher dose than other cancers (median IC50 = 7.35 μM). JQ1 induced G1 cell cycle arrest and premature senescence of OS cells, but produced little apoptosis. Interestingly, c-myc protein levels in JQ1-treated cells remained unchanged, whereas the upregulation of p21 protein was still observable, as noted by Western blots. Although effective in vitro, JQ1 failed to reduce the size of the MNNG/HOS xenografts in immunocompromised mice in vivo. To overcome the resistance of OS cells to JQ1 treatment, we tested JQ1 in combination with rapamycin. Rapamycin inhibits the assembly of mTOR complexes which is one of the important growth signaling pathways in many cancers including OS. JQ1 and rapamycin synergistically inhibited the growth and survival of OS cells in vitro. The drug combination nearly abrogated expression of c-myc protein, enhanced levels of p21, and induced apoptosis in OS cells in vitro. After 24 days of treatment in vivo, JQ1 (50mg/kg body weight) or rapamycin (1.5 mg/kg body weight) alone showed little effect on the size of human OS xenografts in nude mice. However, combination of both drugs significantly inhibited tumor growth by 85% and 73% (p&lt;0.05 for both) compared to either JQ1 or rapamycin alone, respectively. In conclusion, JQ1 inhibited proliferation and survival of OS cells in vitro but failed to reduce growth of OS xenografts in immunodeficient mice. When combined with rapamycin, JQ1 synergistically inhibited proliferation of OS cells both in vitro and in vivo. Citation Format: Dhong Hyun Tony Lee, Jun Qi, James Bradner, Jonathan Said, Ngan Doan, Charles Forscher, H Phillip Koeffler. Synergistic effect of JQ1 and rapamycin for treatment of human osteosarcoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1689. doi:10.1158/1538-7445.AM2014-1689
Identification of the antivasopermeability effect of pigment epithelium-derived factor and its active site (2004)

Vascular permeability plays a key role in a wide array of life-threatening and sight-threatening diseases. Vascular endothelial growth factor can increase vascular permeability. Using a model system for nonproliferative diabetic retinopathy, we found that pigment epithelium-derived factor (PEDF) effectively abated vascular endothelial growth factor-induced vascular permeability. A 44-amino acid region of PEDF was sufficient to confer the antivasopermeability activity. Additionally, we identified four amino acids (glutamate-101, isoleucine-103, leucine-112, and serine-115) critical for this activity. PEDF, or a derivative, could potentially abate or restore vision loss from diabetic macular edema. Furthermore, PEDF may represent a superior therapeutic approach to sepsis-associated hypotension, nephrotic syndrome, and other sight-threatening and life-threatening diseases resulting from excessive vascular permeability.
An Effective Preamble-Based Channel Estimation Structure for OFDM/OQAM Systems (2010)

In this paper, an effective preamble structure with only one zero-value guard symbols for OFDM/OQAM systems is proposed to improve spectrum efficiency. Moreover, a preamble interference cancellation (PIC) based channel estimation (CE) scheme is proposed to mitigate the residual ISI in OFDM/OQAM systems. Simulation results prove that the proposed preamble with PIC based channel estimation is an effective structure for OFDM/OQAM systems.
Reliability Analysis of Self-Repairable MEMS Accelerometer (2006)

MEMS (micro electromechanical system) yield and reliability have been a very critical issue. In our previous paper, the authors have proposed a self-repairable MEMS comb accelerometer device, and the yield analysis has demonstrated effective yield increase due to the BISR (built-in self-repair) design. In this paper, the authors developed a MEMS reliability model for quantitative assessment of the MEMS reliability analysis. Based on this model, analysis of the reliability of both non-BISR and BISR MEMS comb accelerometers under z-axis shocking environment. Simulation results demonstrate very effective reliability enhancement due to the BISR design. The reliability model can also be applied to other MEMS devices under various failure mechanisms in a similar way
A hydroxymethylglutaryl-CoA reductase inhibitor synthesized by yeasts (1995)

Screening of different yeast species showed that they are able to synthesize hydroxymethylglutaryl-CoA (HMGCoA) reductase inhibitors. Crude methanol extracts and the purified inhibitors from Pichia labacensis and Candida cariosilignicola were tested for their biological activity on the solubilized microsomal HMGCoA reductase from Chinese hamster ovary cells. Identification of the inhibitors was studied by thin layer chromatography, high pressure liquid chromatography and mass spectroscopy.
Cystic Fibrosis Gene Mutation in Two Sisters with Mild Disease and Normal Sweat Electrolyte Levels (1991)

CYSTIC fibrosis is the most common fatal recessive disease in whites. In the past the diagnosis has been based on the presence of lung disease, pancreatic exocrine insufficiency, and increased sweat electrolyte concentrations.1 Patients with cystic fibrosis have well-defined abnormalities in epithelial tissue, including defective cyclic AMP—mediated regulation of chloride channels and an increased transepithelial potential difference, which reflects increased sodium absorption and decreased chloride permeability.2 3 4 5 6 The raised sweat chloride values that result from impaired reabsorption of salt in the sweat ducts have been exploited in the pilocarpine iontophoresis test, the cornerstone of diagnosis since 1959.7 Although the vast majority . . .
A comparative investigation of bone surface after cutting with mechanical tools and Er:YAG laser (2015)

We concluded that our new finding with the mechanical osteotomy suggests a biological explanation to the expected difference in subsequent bone healing. Our hypothesis is that the difference of surface characteristic yields to different bleeding pattern and subsequently results in different bone healing. The analyses of bone healing will support our hypothesis.
The role of packaging in achieving microwave browning and crisping (1989)

Abstract Microwave interactive packaging is a rapidly expanding and evolving technology. This class of package contains heater elements (susceptors or receptors) which convert microwave energy into conventional heat, thereby providing useful browning and crisping functions. This paper discusses the principles of microwave interactive package design.
ISLUS: An Immediate and Safe Live Update System for C Program (2017)

Traditional software updating techniques usually needs to shut down the applications, which make them not applicable for applications that needs continuous service. Dynamic software updating (DSU) can update the running programs without downtime, but the existing DSU systems cannot combine flexibility, immediacy and security when they update the software. This paper presents an Immediate and Safe Live Update System (ISLUS) for C program, which aims to update the multi-threaded program securely and immediately without downtime or extra assistance of the special compiler. To solve the semantic security problem when other DSU systems update the functions being called on the stack, ISLUS makes a novel intermediate function as a transition from the old function to the new function. To guarantee the reliability of DSU, which has many dangerous memory operations, ISLUS proposes a rollback mechanism based on checkpoint that can be used to restore the crashed program, which cannot be achieved by the other DSU systems. We provide an example to explain ISLUS and do the security analysis. We have prototyped our system and deployed ISLUS to update two big data applications: Memcached and Redis. The results indicate that ISLUS is effective and with low-overhead.
Power diodes with active control of emitter efficiency (2002)

In bipolar devices, active control of emitter efficiency allows an improved compromise between the on-state behaviour and the turn-off characteristics. This has been previously demonstrated in lateral IGBTs and in MOSFET type diode devices operating in the third quadrant. To avoid serious trigger problems occuring in MOSFET type devices, we propose a novel anode emitter structure for diodes with controlled emitter efficiency (Emitter Controlled Diode, ECD). This is achieved by an additional gate terminal, which controls the minority carrier extraction in the p-emitter via a MOS inversion channel. A suitable pretrigger time interval of the gate signal has been determined. It has also been demonstrated that though introducing a parasitic npn transistor, the safe operation at hard turn-off is not seriously affected.
A Passive Wireless Multi-Sensor SAW Technology Device and System Perspectives (2013)

This paper will discuss a SAW passive, wireless multi-sensor system under development by our group for the past several years. The device focus is on orthogonal frequency coded (OFC) SAW sensors, which use both frequency diversity and pulse position reflectors to encode the device ID and will be briefly contrasted to other embodiments. A synchronous correlator transceiver is used for the hardware and post processing and correlation techniques of the received signal to extract the sensor information will be presented. Critical device and system parameters addressed include encoding, operational range, SAW device parameters, post-processing, and antenna-SAW device integration. A fully developed 915 MHz OFC SAW multi-sensor system is used to show experimental results. The system is based on a software radio approach that provides great flexibility for future enhancements and diverse sensor applications. Several different sensor types using the OFC SAW platform are shown.