gebhart/patrepeval huggingface dataset:
Non-Invasive Imaging of Cardiac Activation and Recovery (2009)

The sequences of activation and recovery of the heart have physiological and clinical relevance. We report on progress made over the last years in the method that images these timings based on an equivalent double layer on the myocardial surface serving as the equivalent source of cardiac activity, with local transmembrane potentials (TMP) acting as their strength. The TMP wave forms were described analytically by timing parameters, found by minimizing the difference between observed body surface potentials and those based on the source description. The parameter estimation procedure involved is non-linear, and consequently requires the specification of initial estimates of its solution. Those of the timing of depolarization were based on the fastest route algorithm, taking into account properties of anisotropic propagation inside the myocardium. Those of recovery were based on electrotonic effects. Body surface potentials and individual geometry were recorded on: a healthy subject, a WPW patient and a Brugada patient during an Ajmaline provocation test. In all three cases, the inversely estimated timing agreed entirely with available physiological knowledge. The improvements to the inverse procedure made are attributed to our use of initial estimates based on the general electrophysiology of propagation. The quality of the results and the required computation time permit the application of this inverse procedure in a clinical setting.
Enzymatic Amplification of Hepatitis B Virus DNA in Serum Compared with Infectivity Testing in Chimpanzees (1989)

The in vivo infectivity titration of hepatitis B virus (HBV) has been biologically standardized in terms of chimpanzee infectious dose (CID50). The polymerase chain reaction (PCR) was used for in vitro enzymatic amplification of HBV DNA and comparison with CID50. Serial dilutions of human serum specimens containing HBV adw (CID50 10(-7] and ayw (CID50 10(-7.5] were tested to determine the reproducibility and sensitivity of PCR for the detection of HBV DNA. The detection limit of HBV DNA in serum using PCR was 10(-8) for either amplification of HBV DNA extracted from serum or direct amplification of HBV DNA in proteolyzed serum. The amplification efficiency of PCR in proteolysates was not significantly reduced by using a 0.25% concentration (vol/vol) of nonionic detergents and 2.5% concentration (vol/vol) of digested human serum. Thus, PCR is a specific and rapid method for in vitro detection of HBV DNA that is more sensitive than the in vivo infectivity titration of HBV by chimpanzee inoculation. Therefore, if HBV-related DNA cannot be detected by PCR in specimens or biologic products, they probably do not contain infectious HBV.
Augmenting the catalytic activity of CoFe<sub>2</sub>O<sub>4</sub> by substituting rare earth cations into the spinel structure (2016)

The current research work evaluates significant enhancement in photo-Fenton activity of pristine cobalt ferrite (CoFe<sub>2</sub>O<sub>4</sub>) by inserting very small quantity of rare earth cations such as cerium (Ce<sup>3+</sup>) and lanthanum (La<sup>3+</sup>) into its spinel structure.
The oxidation of magnetite and related spinels. Constitution of gamma ferric oxide (1956)

I. David and A. J. E. Welch, Trans. Faraday Soc., 1956, 52, 1642 DOI: 10.1039/TF9565201642
The carboxy-terminal region of human lipoprotein lipase is necessary for its exit from the endoplasmic reticulum (1998)

Certain missense substitutions on the human lipase (hLPL) gene produce mutated proteins that are retained in different compartments along the secretory pathway. The purpose of the present study was to elucidate whether the C-terminal domain of the hLPL molecule could be important for secretion. We constructed by site-directed mutagenesis three carboxy-terminal mutated (F388-->Stop, K428-->Stop and K441-->Stop) hLPL cDNAs that were expressed in COS1 cells. Immunoblotting of cell extracts showed that all three constructs led to similar levels of protein. Both wild type (WT) hLPL and the truncated K441-->Stop hLPL were secreted to the extracellular medium, and presented a similar intracellular distribution pattern as shown by immunofluorescence. Neither F388-->Stop nor K428-->Stop hLPL protein was detected in cell medium. Immunofluorescence experiments showed that both truncated hLPL were retained within an intracellular compartment, which became larger. Double immunofluorescence analysis using antibodies against LPL and antiprotein disulfide isomerase as a marker showed that the truncated K428-->Stop hLPL was retained within the rough endoplasmic reticulum. This truncated protein was not found in other compartments in the secretory pathway, such as Golgi complex and lysosomes, indicating that it did not exit the endoplasmic reticulum. Further analysis of the C-terminal region of the LPL molecular model showed both that F388-->Stop and K428-->Stop hLPL truncated proteins are highly hydrophobic. As retention of secretory proteins in the rough endoplasmic reticulum is a quality control mechanism of the secretory pathway, we conclude that the C-terminal domain of hLPL is critical for correct intracellular processing of the newly synthesized protein.
Study of the effect of midwife outpatient service on pregnancy outcome in pregnant women from floating population (2009)

Objective To investigate the effect of midwife outpatient service on pregnancy outcome in pregnant women from floating population. Methods The data of 6236 pregnant and parturient women who were from floating population from July 2006 to July 2008 in our hospital were analyzed retrospectively. Among them 3 076 cases from July 2006 to July 2007 (before implementing floating population management) were as control group, and 3 160 cases from July 2007 to July 2008 (after implementing floating population management) were as observation group. The frequency of antenatal examination, satisfaction and adverse pregnancy outcome between two groups were compared. Results The frequency of antenatal examination, the rates of hospital childbirth, natural labor and satisfaction in observation group were obviously higher than those in the control group (P 0.05). Conclusions Using the midwife outpatient service and strengthening antenatal examination of pregnant and parturient women and follow-up could effectively reduce the incidence rate of adverse pregnancy outcome of floating population.

Key words: 
Midwife;  Floating population;  Antenatal cara;  Pregnancy out come
Lymphocyte recognition of lymph node high endothelium. VII. Cell surface proteins involved in adhesion defined by monoclonal anti-HEBFLN (A.11) antibody. (1985)

Lymphocyte entry into lymph nodes (LN) and Peyer's patches (PP) occurs specifically at high endothelial cell venules (HEV). We previously isolated a high endothelial binding factor (HEBFLN) from rat lymph that blocked the lymphocyte binding sites of HEVLN but not HEVPP. In this study, mouse monoclonal anti-HEBFLN antibody (A.11) was used to investigate rat lymphocyte surface structures mediating adhesion to high endothelium. The A.11 antigen was expressed on the majority of thoracic duct lymphocytes (TDL), spleen, LN, PP cells, but was only detected on few (1 to 10%) thymus and bone marrow cells (indirect immunofluorescence). The treatment of TDL with the A.11 IgG blocked their ability to bind to HEVLN. This effect was specific, inasmuch as A.11 antibody did not block lymphocyte binding to HEVPP, and an anti-leukocyte-common antigen monoclonal antibody, OX1, did not block lymphocyte binding to HEVLN. In addition, the A.11 antigen isolated from the lymph and detergent lysates of TDL by antibody affinity chromatography had the capacity to block the lymphocyte binding sites of HEVLN but not HEVPP. Immunoprecipitation studies revealed that the A.11 antibody recognized the radioiodinated surface membrane proteins of TDL and TDL-derived T cells and B cells, which resolved with SDS-PAGE autoradiography into three polypeptides with relative m.w. of approximately 135,000, 63,000, and 40,000. We conclude that the A.11 antigen is a component of the lymphocyte surface recognition structure that mediates adhesion to high endothelial cells of rat peripheral lymph nodes.
NALOXONE DECREASES FOOD INTAKE IN OBESE HUMANS (1982)

Food intake decreased with a 15 mg naloxone infusion in obese humans but not in lean controls or obese subjects given 2 mg. Glucose and insulin levels did not differ from control during naloxone infusion alone or with meal stimulation. Endogenous opiates may contribute to obesity in humans but probably not through changes in insulin secretion.
Microbial Biosensors Based on Respiratory Inhibition (2003)

Biosensors generally provide a rapid and convenient alternative to conventional methods for monitoring chemical substances in fields as diverse as medicine, environmental monitoring, and food processing. A biosensor is a device that combines a biologic-sensing element with an electronic signal-transducing element. As biologic-sensing elements, enzymes, antibodies, receptors, organelles, and microorganisms, as well as animal and plant cells, can be used.
Integrative Model for Binding of <i>Bacillus thuringiensis</i> Toxins in Susceptible and Resistant Larvae of the Diamondback Moth ( <i>Plutella xylostella</i> ) (1999)

Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth (Plutella xylostella) is the first insect to evolve resistance to B. thuringiensis in open-field populations. The only known mechanism of resistance to B. thuringiensis in the diamondback moth is reduced binding of toxin to midgut binding sites. In the present work we analyzed competitive binding of B. thuringiensis toxins Cry1Aa, Cry1Ab, Cry1Ac, and Cry1F to brush border membrane vesicles from larval midguts in a susceptible strain and in resistant strains from the Philippines, Hawaii, and Pennsylvania. Based on the results, we propose a model for binding of B. thuringiensis crystal proteins in susceptible larvae with two binding sites for Cry1Aa, one of which is shared with Cry1Ab, Cry1Ac, and Cry1F. Our results show that the common binding site is altered in each of the three resistant strains. In the strain from the Philippines, the alteration reduced binding of Cry1Ab but did not affect binding of the other crystal proteins. In the resistant strains from Hawaii and Pennsylvania, the alteration affected binding of Cry1Aa, Cry1Ab, Cry1Ac, and Cry1F. Previously reported evidence that a single mutation can confer resistance to Cry1Ab, Cry1Ac, and Cry1F corresponds to expectations based on the binding model. However, the following two other observations do not: the mutation in the Philippines strain affected binding of only Cry1Ab, and one mutation was sufficient for resistance to Cry1Aa. The imperfect correspondence between the model and observations suggests that reduced binding is not the only mechanism of resistance in the diamondback moth and that some, but not all, patterns of resistance and cross-resistance can be predicted correctly from the results of competitive binding analyses of susceptible strains.
Development of Low-Cost Portable Spectrometers for Detection of Wood Defects (2020)

Portable spectroscopic instruments are an interesting alternative for in-field and on-line measurements. However, the practical implementation of visible-near infrared (VIS-NIR) portable sensors in the forest sector is challenging due to operation in harsh environmental conditions and natural variability of wood itself. The objective of this work was to use spectroscopic methods as an alternative to visual grading of wood quality. Three portable spectrometers covering visible and near infrared range were used for the detection of selected naturally occurring wood defects, such as knots, decay, resin pockets and reaction wood. Measurements were performed on wooden discs collected during the harvesting process, without any conditioning or sample preparation. Two prototype instruments were developed by integrating commercially available micro-electro-mechanical systems with for-purpose selected lenses and light source. The prototype modules of spectrometers were driven by an Arduino controller. Data were transferred to the PC by USB serial port. Performance of all tested instruments was confronted by two discriminant methods. The best performing was the microNIR instrument, even though the performance of custom prototypes was also satisfactory. This work was an essential part of practical implementation of VIS-NIR spectroscopy for automatic grading of logs directly in the forest. Prototype low-cost spectrometers described here formed the basis for development of a prototype hyperspectral imaging solution tested during harvesting of trees within the frame of a practical demonstration in mountain forests.
Neuroprotective Strategies to Avert Seizure‐Induced Neurodegeneration in Epilepsy (2007)

Neurodegeneration in limbic circuits is a hallmark feature of chronic temporal lobe epilepsy (TLE). Studies in experimental animal models and human patients indicate that seizure-induced neuronal injury involves some active, as well as passive cell death processes. Experimental approaches that inhibit active steps in cell death programs have been shown to reduce neuronal cell death and sclerosis, but not to prevent epileptogenesis in animal models of TLE. These findings suggest that we need additional research using both animal models and brain slices from human patients to understand the pathological mechanisms underlying seizure generation. Such comparative studies will also aid in evaluating the potential therapeutic value of inhibiting cell death in seizure disorders.
Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators. (1992)

Manganese oxidation by manganese peroxidase (MnP) was investigated. Stoichiometric, kinetic, and MnII binding studies demonstrated that MnP has a single manganese binding site near the heme, and two MnIII equivalents are formed at the expense of one H2O2 equivalent. Since each catalytic cycle step is irreversible, the data fit a peroxidase ping-pong mechanism rather than an ordered bi-bi ping-pong mechanism. MnIII-organic acid complexes oxidize terminal phenolic substrates in a second-order reaction. MnIII-lactate and -tartrate also react slowly with H2O2, with third-order kinetics. The latter slow reaction does not interfere with the rapid MnP oxidation of phenols. Oxalate and malonate are the only organic acid chelators secreted by the fungus in significant amounts. No relationship between stimulation of enzyme activity and chelator size was found, suggesting that the substrate is free MnII rather than a MnII-chelator complex. The enzyme competes with chelators for free MnII. Optimal chelators, such as malonate, facilitate MnIII dissociation from the enzyme, stabilize MnIII in aqueous solution, and have a relatively low MnII binding constant.
Real-time inter-processing for pc based robot controllers in tele-robotic and gantry robotic controller system (2009)

High performance robot control systems are essential to control with fully deterministic and reliable processes. To provide such a versatile and high performance robot control platform, an embedded robot controller based on parallel or inter-processes computing concept will be proposed In this paper presents the development of real-time controllers for robot controllers which features high computational power. An inter-process programming concept with real-time operating system are show which it can perform successfully the advanced requirement such as a 6 DOF tele-robotic and gantry robotic system.
Rapid Sara Seprations by High Performance Liqid Chromatography (1980)

Abstract This paper presents both rapid analytical and preparative high performance liquid chromatographic (HPLC) techniques for separating liquid-fuel type materials into saturates, aromatics, resins, and asphaltenes (SARA). The preparative method, an adaptation of a technique developed by Jewell, et. al. (1, 4), significantly decreases analysis time. The analytical technique utilizes HPLC to achieve the same separations in less time.
Prelaunch characteristics of the Moderate Resolution Imaging Spectroradiometer (MODIS) on EOS-AM1 (1998)

The Moderate Resolution Imaging Spectroradiometer (MODIS), with 36 bands and 0.25-, 0.5-, and 1.0-km geometric instantaneous-fields-of-view (GIFOVs) at nadir, has completed system level testing and has been integrated onto the Earth Observing System (EOS)-AM1 spacecraft, which is slated for launch in 1998. Raytheon Santa Barbara Remote Sensing (SBRS), Goleta, CA, the MODIS developer, has performed extensive characterization and calibration measurements that have demonstrated a system that meets or exceeds most of NASA's demanding requirements. Based on this demonstrated capability, the MODIS Science Team, an international group of 28 land, ocean, atmosphere, and calibration remote-sensing scientists, has commenced delivery of algorithms that will routinely calculate 42 MODIS standard data products postlaunch. These products range from atmospheric aerosols, snow cover, and land and water surface temperature to leaf area index, ocean chlorophyll concentration, and sea ice extent, to name just a few. A description of the Science Team, including members' research interests and descriptions of their MODIS algorithms, can be found at the MODIS homepage (http://ltpwww.gsfc.nasa.gov/MODIS/MODIS.html). The MODIS system level testing included sufficient measurements in both ambient and thermal-vacuum environments to both demonstrate specification compliance and enable postlaunch implementation of radiometric calibration algorithms. The latter will include calculations to account for changes in response versus scan angle, response versus temperature, and response linearity. The system level tests also included performance verification of the onboard calibration systems, including the solar diffuser stability monitor (SDSM), the blackbody (BB), and the spectral radiometric calibration assembly (SRCA), which will enahle monitoring of MODIS performance postlaunch. Descriptions of these subsystems are also on the MODIS homepage.
Dispersion of microwave-modulated optical signals (1993)

Experimental and analytical evaluation of dispersion effects that control intersymbol interference in gigabit, single-mode-fiber, digital systems is described. Both direct modulation of distributed-feedback (DFB) lasers and external modulation by Mach-Zehnder modulators are studied. The experiment uses sinusoidal modulation of the light at a microwave frequency, e.g. 5 GHz, and the subsequent measurement of signal gain or loss in a dispersive fiber. For DFB lasers, the transmission measurements are used to derive the transient and adiabatic components of chirp. It is shown that, for external modulation at 5 GHz, the 1.55- mu m optical signals suffer relatively little dispersion loss for transmission distances up to 60 km. Analytical solutions for waveform distortion are given for external modulation and show good agreement with measurements.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Photonic Devices Based on Crystalline Organic Semiconductors for Optoelectronic Integrated Circuits (1995)

Crystalline organic thin films are deposited under ultrahigh vacuum conditions at substrate temperatures from 77 K to 300 K. Due to the weak van der Waals bonding forces between the organic molecules, lattice matching is not required for layer growth. Structures can be prepared both on dielectric or amorphous substrates and on top of completely processed III-V or Si wafers. In this paper, we discuss the fabrication and performance of organic semiconductor electroluminescent devices, waveguides, and photodetectors.
Synaptic Cell Adhesion (2012)

Chemical synapses are asymmetric intercellular junctions that mediate synaptic transmission. Synaptic junctions are organized by trans-synaptic cell adhesion molecules bridging the synaptic cleft. Synaptic cell adhesion molecules not only connect pre- and postsynaptic compartments, but also mediate trans-synaptic recognition and signaling processes that are essential for the establishment, specification, and plasticity of synapses. A growing number of synaptic cell adhesion molecules that include neurexins and neuroligins, Ig-domain proteins such as SynCAMs, receptor phosphotyrosine kinases and phosphatases, and several leucine-rich repeat proteins have been identified. These synaptic cell adhesion molecules use characteristic extracellular domains to perform complementary roles in organizing synaptic junctions that are only now being revealed. The importance of synaptic cell adhesion molecules for brain function is highlighted by recent findings implicating several such molecules, notably neurexins and neuroligins, in schizophrenia and autism.
The Downstream Regulatory Element of the proU Operon of Salmonella typhimurium Inhibits Open Complex Formation by RNA Polymerase at a Distance (2000)

The intracellular concentration of K(+)-glutamate, chromatin-associated proteins, and a downstream regulatory element (DRE) overlapping with the coding sequence, have been implicated in the regulation of the proU operon of Salmonella typhimurium. The basal expression of the proU operon is low, but it is rapidly induced when the bacteria are grown in media of high osmolarity (e.g. 0.3 M NaCl). It has previously been suggested that increased intracellular concentrations of K(+)-glutamate activate the proU promoter in response to increased extracellular osmolarity. We show here that the activation of the proU promoter by K(+)-glutamate in vitro is nonspecific, and the in vivo regulation cannot simply be mimicked in vitro. In vivo specificity requires both the chromatin-associated protein H-NS and the DRE; they are both needed to maintain repression of proU expression at low osmolarity. How H-NS and the DRE repress the proU promoter in vivo has so far been unclear. We show that, in vivo, the DRE acts at a distance to inhibit open complex formation at the proU promoter.
Separate binding sites for nuclear factor 1 and a CCAAT DNA binding factor in the mouse alpha 2(I) collagen promoter. (1987)

A factor present in nuclear extracts of NIH-3T3 fibroblasts was identified which binds to a region between -315 and -295 with respect to the start of transcription in the mouse alpha 2(I) collagen promoter. This factor was assayed using three different DNA binding assays. Evidence that this factor is nuclear factor 1 or a related protein is based on competition experiments using fragments of another promoter that contains a nuclear factor 1 binding site as well as a fragment containing a consensus sequence for nuclear factor 1 binding sites. The nucleotide sequence of the binding site in the alpha 2(I) promoter. TCGN5GCCAA, presents similarities to the sequence published previously as a consensus recognition sequence for nuclear factor 1. Methylation interference experiments indicate that the factor interacts at least with two successive G residues in the major groove complementary to the two successive C residues in the GCCAA motif. This factor is different from a factor that binds to a CCAAT element between -84 and -80 in the mouse alpha 2(I) collagen promoter. This was shown by competition experiments using both crude NIH-3T3 fibroblast nuclear extracts and a partially purified CCAAT binding factor. These results suggest that in the alpha 2(I) collagen promoter nuclear factor 1 and a CCAAT binding factor bind to separate sites, despite analogies in their recognition sequences.