gebhart/patrepeval huggingface dataset:
Abstract 5542: Annexin A5 Reduces Inflammation Mediated Vascular Remodelling and Post-interventional Atherosclerosis and Improves Vascular Function in Hypercholesterolemic Mice (2009)

Annexin A5 is abundant in atherosclerotic plaques, has anti-thrombotic properties, and it may reduce ischaemia-reperfusion injury. We showed that annexin A5 could reduce neointima development in mouse models of vein graft restenosis and cuff induced neointima formation in hypercholesterolemic ApoE3*Leiden mice. These effects were associated to a reduction in vessel wall leucocytes, indicating an anti-inflammatory effect. Here we further investigated if annexin A5 possess anti-inflammatory properties, and can affect functional consequences of inflammation in the vessel wall. Methods. Plasma cytokines were measured by multiplex immunoassay in hypercholesterolemic ApoE3*Leiden mice 28 days after the placement of a vein graft in the carotid artery. Mice were treated with 1 mg/kg bw of annexin A5 or placebo once daily by intraperitoneal injection. In a second experiment, ApoE (−/−) mice were given a western diet for 16 weeks, after which they were treated with annexin A5 or placebo for 3 days, as above. They were then anaesthetised (isoflurane) and a Samba Preclin pressure transducer was introduced into the aortic arch through a carotid artery. Endothelium mediated dilatation was induced by intraperitoneal injection of 3 μ g metacholine. Results. Plasma levels of G-CSF, IFN γ and MIP-1 β /CCL4 were significantly reduced by annexin A5 28 days after surgery. Vein graft remodelling was reduced by 48±12% in the annexin A5 treated mice. In apoE (−/−) mice, diastolic and systolic blood pressure were not significantly different. Placebo treated mice responded to metacholine with a minor reduction in diastolic (−1 mmHg at 3 minutes after injection) and systolic blood pressure (−5 mmHg). In contrast, annexin A5 treated mice responded with a pronounced and more long-lasting reduction in diastolic (− 11 mmHg) and systolic (−22 mmHg) blood pressures, p < 0.05 for both pressures. Conclusions. Annexin A5 has systemic anti-inflammatory properties, as shown by reduced plasma cytokine levels, as well as it (as previously shown) blocks vascular inflammation in disease models. It can also normalise disease related impairment of endothelium mediated dilatation. Annexin A5 may have therapeutic benefits in inflammation associated vascular disease.
Mercury removal from water by iron sulfide minerals. An electron spectroscopy for chemical analysis (ESCA) study (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMercury removal from water by iron sulfide minerals. An electron spectroscopy for chemical analysis (ESCA) studyJames R. Brown, G. Michael Bancroft, William S. Fyfe, and Ronald A. N. McLeanCite this: Environ. Sci. Technol. 1979, 13, 9, 1142–1144Publication Date (Print):September 1, 1979Publication History Published online1 May 2002Published inissue 1 September 1979https://pubs.acs.org/doi/10.1021/es60157a013https://doi.org/10.1021/es60157a013research-articleACS PublicationsRequest reuse permissionsArticle Views537Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Lateral aberration measurements with a digital Talbot interferometer (1984)

A new technique for computer-based Talbot interferometry is presented. Fast and automatic aberration measurement has been achieved with improved accuracy and sensitivity. Experimental results are given that agree with the results of calculation by ray tracing.
Development of a Safe and Effective One-Minute Preoperative Skin Preparation (1985)

Three consecutive controlled randomized clinical trials utilizing 1,324 patients were conducted to study the efficacy of incise drapes to prevent wound infections. When a polyester antimicrobial incise drape (loban 2 Antimicrobial Film) was applied to an operative area after a one-minute skin preparation using either 70% alcohol or 2% iodine in 90% alcohol, the clean wound infection rate (1.3%) and overall wound infection rate (2.5%) were comparable to those following a standard ten-minute skin preparation with Betadine (1.3% and 2.3%, respectively). During preliminary studies, it was demonstrated that separation of polyethylene antimicrobial incise drapes from the skin during operation was associated with a sixfold increase in infection rate when compared with operations in which the incise drape did not lift. Design of the drape and technique of application are important considerations in preventing lift from the skin.
NITRIC OXIDE AND PEROXYNITRITE MEDIATED ACTIVATION OF POLY-ADF RIBOSYL SYNTHETASE CAUSES DEPLETION OF CELLULAR ENERGY STORES ??? A NOVEL PATHWAY IN THE PATHOGENESIS OF CIRCULATORY SHOCK (1995)

Division of Critical Care, Children's Hospital Medical Center,3333 Burnet Avenue, Cincinnati, OH, 45229
Cell Cycle Synchronization at the G<sub>2</sub>/M Phase Border by Reversible Inhibition of CDK1 (2006)

Chemical agents for cell cycle synchronization have greatly facilitated the study of biochemical events driving cell cycle progression. G1, S and M phase inhibitors have been developed and used widely in cell cycle research. However, currently there are no effective G2 phase inhibitors and synchronization of cultured cells in G2 phase has been challenging. Recently, a selective CDK1 inhibitor, RO-3306, has been identified that reversibly arrests proliferating human cells at the G2/M phase border and provides a novel means for cell cycle synchronization. A single-step protocol using RO-3306 permits the synchronization of >95% of cycling cancer cells in G2 phase. RO-3306 arrested cells enter mitosis rapidly after release from the G2 block thus allowing for isolation of mitotic cells without microtubule poisons. RO-3306 represents a new molecular tool for studying CDK1 function in human cells.
Primary glia expressing the G93A-SOD1 mutation present a neuroinflammatory phenotype and provide a cellular system for studies of glial inflammation (2006)

Detailed study of glial inflammation has been hindered by lack of cell culture systems that spontaneously demonstrate the "neuroinflammatory phenotype". Mice expressing a glycine --> alanine substitution in cytosolic Cu, Zn-superoxide dismutase (G93A-SOD1) associated with familial amyotrophic lateral sclerosis (ALS) demonstrate age-dependent neuroinflammation associated with broad-spectrum cytokine, eicosanoid and oxidant production. In order to more precisely study the cellular mechanisms underlying glial activation in the G93A-SOD1 mouse, primary astrocytes were cultured from 7 day mouse neonates. At this age, G93A-SOD1 mice demonstrated no in vivo hallmarks of neuroinflammation. Nonetheless astrocytes cultured from G93A-SOD1 (but not wild-type human SOD1-expressing) transgenic mouse pups demonstrated a significant elevation in either the basal or the tumor necrosis alpha (TNFalpha)-stimulated levels of proinflammatory eicosanoids prostaglandin E2 (PGE2) and leukotriene B4 (LTB4); inducible nitric oxide synthase (iNOS) and *NO (indexed by nitrite release into the culture medium); and protein carbonyl products. Specific cytokine- and TNFalpha death-receptor-associated components were similarly upregulated in cultured G93A-SOD1 cells as assessed by multiprobe ribonuclease protection assays (RPAs) for their mRNA transcripts. Thus, endogenous glial expression of G93A-SOD1 produces a metastable condition in which glia are more prone to enter an activated neuroinflammatory state associated with broad-spectrum increased production of paracrine-acting substances. These findings support a role for active glial involvement in ALS and may provide a useful cell culture tool for the study of glial inflammation.
High-Yield, In Vitro Protein Expression Using a Continuous-Exchange, Coupled Transcription/Translation System (2001)

The Rapid Translation System (RTS 500) (Roche Molecular Biochemicals) is a high-yield protein expression system that utilizes an enhanced E. coli lysate for an in vitro transcription/translation reaction. In contrast to conventional transcription/translation, this system allows protein expression to continue for more than 24 h. We demonstrated the utility of the RTS 500 by expressing different soluble and active proteins that generally pose problems in cell-based expression systems. We first expressed GFP-lunasin, a fusion protein that, because of its toxicity, has been impossible to produce in whole cells. The second protein we expressed, human interleukin-2 (IL-2), is generally difficult to produce, either as the native molecule or as a GSTfusion protein, in a soluble form in bacteria. Finally, we demonstrated the capacity of the RTS 500 to co-express proteins, by the simultaneous production of GFP and CAT in a single reaction. This new technology appears to be particularly usefulfor the convenient production of preparative amounts (100-900 microg) of proteins that are toxic or insoluble in cell-based systems.
Case Based Learning in Microbiology (2014)

Whenever we try to introduce any new method, we need to put in lot of efforts. The experience may not be smooth selling every time, and one needs to be prepared for the same. But few things I learned, most of the teacher colleagues cooperate so concentrate on them and not on those who show resistance. Any new method will not be accepted initially but if it proved to be good and useful will be accepted after initial resistance.
Application of High Current Arsenic Ion Implantation to Dynamic MOS Memory LSI's (1979)

Application of high current arsenic ion implantation technology to the fabrication of MOS LSI's is investigated. Substrate temperature rise during ion implantation, carrier profile and residual defect profile after high temperature annealing are measured to solve the fundamental problems involved in high current ion implantation. MOS FET's, junction diodes and 4 K-bit dynamic MOS memory LSI's are fabricated using high current arsenic ion implantation technology. MOS FET characteristics are improved by this implantation technology, when compared with the characteristics of devices fabricated by conventional ampule diffusion technology. The generation-recombination center concentrations derived from junction leakage current measurements are below 1011 cm-3, and are in good agreement with the results for dynamic MOS memory refreshtime.
Mammalian Sperm-Egg Fusion: Evidence That Epididymal Protein DE Plays a Role in Mouse Gamete Fusion1 (2000)

Rat epididymal protein DE associates with the sperm surface during epididymal maturation and is a candidate molecule for mediating gamete membrane fusion in the rat. Here, we provide evidence supporting a role for DE in mouse sperm-egg fusion. Western blot studies indicated that the antibody against rat protein DE can recognize the mouse homologue in both epididymal tissue and sperm extracts. Indirect immunofluorescence studies using this antibody localized the protein on the dorsal region of the acrosome. Experiments in which zona-free mouse eggs were coincubated with mouse capacitated sperm in the presence of DE showed a significant and concentration-dependent inhibition in the percentage of penetrated eggs, with no effect on either the percentage of oocytes with bound sperm or the number of sperm bound per egg. Immunofluorescence experiments revealed specific DE-binding sites on the fusogenic region of mouse eggs. Because mouse sperm can penetrate zona-free rat eggs, the participation of DE in this interaction was also investigated. The presence of the protein during gamete coincubation produced a significant reduction in the percentage of penetrated eggs, without affecting the binding of sperm to the oolemma. These observations support the involvement of DE in an event subsequent to sperm-egg binding and leading to fusion in both homologous (mouse-mouse) and heterologous (mouse-rat) sperm-egg interaction. The lack of disintegrin domains in DE indicates that the protein interacts with its egg-binding sites through a novel mechanism that does not involve the reported disintegrin-integrin interaction.
Reaction of α-(<i>n</i>-Alkylcarbonyloxy)alkyl (ACOA) Halides with 4-Hydroxyacetanilide and 2,2,5,7,8-Pentamethyl-6-chromanol: The Effect of Steric Hindrance on Reaction Path (2008)

A convenient synthesis of α-(n-alkylcarbonyloxy)alkyl (ACOA) iodides has been developed and a homologous series of n-alkylcarbonyloxymethyl (ACOM) iodides have been used to alkylate 4-hydroxyacetanilide (acetaminophen, APAP), a sterically unhindered phenol, and a sterically hindered phenol (2,2,5,7,8-pentamethyl-6-chromanol). Steric hindrance was not a significant factor in the ratio of acylated (Path b) to alkylated (Path a) for these reactions. Given the reported toxicity associated with sterically hindered ACOM prodrugs, n-alkyl ACOM and ACOA promoieties present themselves as viable alternatives to the more commonly used pivalate-based derivatives.
Multiple subsets of HIV‐specific cytotoxic T lymphocytes in humans and in mice (1989)

The human immunodeficiency virus type 1 (HIV-1) induces a strong cytotoxic T lymphocyte (CTL) response in humans following infection. HIV-specific CTL can be detected directly in the blood and lungs of infected patients, and can be expanded in vitro by stimulation with autologous HIV-infected lymphoblasts. Furthermore, CTL specific for HIV envelope glycoprotein gp160 have been obtained in mice by immunization with recombinant vaccinia virus (VV) that carry the HIV env gene. In this study, we show that mice also produce strong CTL responses to gag and nef proteins following immunization with VV recombinants, thus providing a convenient model system to study T lymphocyte immunity to defined HIV antigens. To determine the specificity of circulating HIV-immune CTL in humans, a panel of doubly transfected mouse P815 tumor cells was produced which express the human HLA-A2 or HLA-A3 transplantation antigen gene and one HIV-1 gene (env, gag or nef). Using these cells as targets to CTL, we show that HIV-infected humans carry co-existing CTL sub-populations of different specificities. Each subpopulation appears to vary in intensity among different individuals. Surprisingly, CTL specific for regulatory, non-structural nef protein appear to be a major constituent of the human immune response to HIV.
Best Practices for Verification, Validation, and Test in Model-Based Design (2008)

<div class="htmlview paragraph">Model-Based Design is no longer limited to R&amp;D and pilot programs; it is frequently used for production programs at automotive companies around the world. The demands of production programs drive an even greater need for tools and practices that enable automation and rigor in the area of verification, validation, and test. Without these tools and practices, achieving the quality demanded by the automotive market is not possible. This paper presents best practices in verification, validation, and test that are applicable to any program, but are critical when applying Model-Based Design in production programs.</div>
Simian Immunodeficiency Virus (SIV)<i>gag</i>DNA-Vaccinated Rhesus Monkeys Develop Secondary Cytotoxic T-Lymphocyte Responses and Control Viral Replication after Pathogenic SIV Infection (2000)

The potential contribution of a plasmid DNA construct to vaccine-elicited protective immunity was explored in the simian immunodeficiency virus (SIV)/macaque model of AIDS. Making use of soluble major histocompatibility class I/peptide tetramers and peptide-specific killing assays to monitor CD8(+) T-lymphocyte responses to a dominant SIV Gag epitope in genetically selected rhesus monkeys, a codon-optimized SIV gag DNA vaccine construct was shown to elicit a high-frequency SIV-specific cytotoxic T-lymphocyte (CTL) response. This CTL response was demonstrable in both peripheral blood and lymph node lymphocytes. Following an intravenous challenge with the highly pathogenic viral isolate SIVsm E660, these vaccinated monkeys developed a secondary CTL response that arose with more rapid kinetics and reached a higher frequency than did the postchallenge CTL response in control plasmid-vaccinated monkeys. While peak plasma SIV RNA levels were comparable in the experimentally and control-vaccinated monkeys during the period of primary infection, the gag plasmid DNA-vaccinated monkeys demonstrated better containment of viral replication by 50 days following SIV challenge. These findings indicate that a plasmid DNA vaccine can elicit SIV-specific CTL responses in rhesus monkeys, and this vaccine-elicited immunity can facilitate the generation of secondary CTL responses and control of viral replication following a pathogenic SIV challenge. These observations suggest that plasmid DNA may prove a useful component of a human immunodeficiency virus type 1 vaccine.
Picosecond Q-switched microchip lasers (2002)

Q-switched microchip lasers have the potential to produce pulses of less than 100-ps duration with peak powers in excess of 1 MW. Here the author designs a Nd:YAG microchip laser.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Characterization of <i>Escherichia coli men</i> Mutants Defective in Conversion of <i>o</i> -Succinylbenzoate to 1,4-Dihydroxy-2-Naphthoate (1982)

Four independent menaquinone (vitamin K<sub>2</sub>-deficient mutants of <i>Escherichia coli</i>, blocked in the conversion of o-succinylbenzoate (OSB) to 1,4-dihydroxy-2-naphthoate (DHNA), were found to represent two distinct classes. Enzymatic complementation was observed when a cell-free extract of one mutant was mixed with extracts of any of the remaining three mutants. The missing enzymes in the two classes were identified by in vitro complementation with preparations of OSB-coenzyme A (CoA) synthetase or DHNA synthase isolated from <i>Mycobacterium phlei</i>. Mutants lacking DHNA synthase (and therefore complementing with <i>M. phlei</i> DHNA synthase) were designated <i>menB</i>, and the mutant lacking OSB-CoA synthetase (and therefore complementing with <i>M. phlei</i> OSB-CoA synthetase) was designated <i>menE</i>. The <i>menB</i> mutants produced only the spirodilactone form of OSB when extracts were incubated with [2,3-<sup>14</sup>C<sub>2</sub>]OSB, ATP, and CoA; the OSB was unchanged on incubation with an extract from the <i>menE</i> mutant under these conditions. Experiments with strains lysogenized by a <i>λ men</i> transducing phage (λG68) and transduction studies with phage P1 indicated that the <i>menB</i> and <i>menE</i> genes form part of a cluster of four genes, controlling the early steps in menaquinone biosynthesis, located at 48.5 min in the <i>E. coli</i> linkage map. Evidence was obtained for the clockwise gene order <i>gyrA....menC-E⃰ -B-D</i>, where the asterisk denotes the uncertain position of <i>menE</i> relative to <i>menC</i> and <i>menB</i>. The transducing phage (λG68) contained functional <i>menB</i>, <i>menC</i>, and <i>menE</i> genes, but only part of the <i>menD</i> gene, and it was designated λ <i>menCE⃰ B(D)</i>.
Corps Guidelines for Dam Safety Inspection Need Revamping (1975)

A protest (1) that Proposed Guidelines for Safety Inspection of Dams, Federal Register, August 1974, pp. 31334-31346, ignores well-publicized conclusions of a preexisting ASCE task committee and (2) that safety criteria of the proposed guidelines are overly conservative so that, if implemented, unwarranted millions of human welfare dollars will be wasted. Improved criteria are recommended. Enormity of any dam inspection and improvement program is also flagged. Its dollar cost ignores comparison of resulting values with values of other potential programs affecting human welfare. Its huge manpower need in even a drawn-out, 25-year schedule has no ready solution, but improved criteria would inspire maximum effort by civil engineers.
<title>Effects of high frequency injection and optical feedback on semiconductor laser performance</title> (1990)

At typical read powers, laser intensity fluctuations can be the dominant source of noise in the read channel of an optical drive. Even with the isolation present in most write-once optical heads, optical feedback (OFB) can be a significant contributor to the noise in the system read channel. The dominant laser noise sources are mode hopping and mode partition noise.' The former noise is particularly troublesome as it results from an alternation of the dominant laser mode, which causes output power fluctuations and irreproducible playback performance. At certain combinations of drive current, temperature, and optical feedback, this unstable behavior is observed. The second contributor, mode partition noise, is the broad-band noise associated with the transient variation in the partition of energy per mode.
Molecular detection of anthrax spores on animal fibres (2003)

The rapid diagnostic test could be applied to the analysis of commercial fibre samples for the detection of anthrax as required by health and safety legislation resulting in considerable savings in time and expense.
Preferential Growth of Cube-Oriented Grains in Partially Annealed and Additionally Rolled Aluminum Foils for Capacitors (2004)

It is well known that a sharp Cube texture is developed for the producing process of a pure aluminum foil for capacitors. The orientation analysis using scanning electron microscopy/electron backscattered diffraction pattern (SEM/EBSP) method has been performed on the transverse direction (TD) sections of partially annealed (PAed) and additionally rolled (ARed) foils through the thickness in order to reveal how Cube-oriented grains have advantages for growth in comparison with the other grains with orientation components of rolling texture. Samples were annealed repeatedly with increasing the temperature in a vacuum. The Cube grains were distributed throughout foil thickness homogeneously in the PAed and ARed foils. The main mechanism of preferential growth of the Cube grains in the PAed foils was driven by grain boundary energy. However, the growth for ARed foils was caused by strain induced grain boundary migration (SIBM) driven by the difference in stored energy between the Cube and the other grains, because the former store less strain energy than the latter after the additional rolling. Furthermore, the grown-up Cube grains having similar orientations met each other and made a cluster in both the foils. Since the clusters develop to reach the foil surfaces and become stable, the Cube grains are easy to cover all of the foil in the final stage of annealing.