gebhart/patrepeval huggingface dataset:
Flow cytometric analysis of <i>Chlamydia trachomatis</i> interaction with L cells (1987)

Immunofluorescent staining and flow cytometric analysis have been investigated as means of studying the early stages of in vitro infection of Chlamydia trachomatis. The lymphogranuloma venereum strain of C. trachomatis was grown in vitro in L cells, fixed in p-formaldehyde, stained with fluorescein isothiocyanate (FITC)-conjugated monoclonal antibody to the chlamydial major outer membrane protein, and analyzed flow cytometrically. Infected cells stained 50-100 times more intensely than uninfected cells, and they could easily be discriminated by flow analysis. The number of infected cells and the fluorescence intensity of individual cells were proportional to the multiplicity of infection. The attachment of purified elementary bodies to L cells could be analyzed by immunofluorescence and flow cytometry. Cells exposed to 0.26 inclusion-forming units/cell could be discriminated from an unexposed population. Flow analysis of purified elementary bodies was possible after fluorescent staining with the aid of a laser-based cytometer and gating on low volume.
Data Sharing for Cloud Computing Platforms (2014)

Cloud computing platforms consist of a set of reliable services that are run in the cloud. Typically, consumer applications use software development kits (SDKs) provided by the computing platform services to store, update, and retrieve instances of data in the cloud. Services provided by the cloud computing platform, expose different data modeling paradigms that consumer applications use to interact with the cloud. The service-specific data modeling paradigms and SDKs increase the complexity of data sharing between consumer applications that interact with the different services of the cloud computing platform. To make matters more complicated, it's not uncommon in an enterprise to find different groups using different cloud computing platforms. In this paper, we will describe a set of abstractions that can be used to abstract different computing platforms. The abstractions not only abstract the computing platform, but also enable the data discovery and sharing between applications. We will further show that these abstractions do not add substantial latency on the performance of the computing platform.
Triazine Derivatives Inhibit Rat Hepatocarcinogenesis but Do Not Enhance Gap Junctional Intercellular Communication (1997)

We report here novel candidate chemopreventive agents active against experimental hepatocarcinogenesis. The triazine derivatives 6-(2-chlorophenyl)-2,4-diamino-1,3,5-triazine (2CPDAT), 6-(3-chlorophenyl)-2,4-diamino-1,3,5-triazine (3CPDAT), 6-(4-chlorophenyl)-2,4-diamino-1,3,5-triazine (4CPDAT), 6-(4-pyridyl)-2,4-diamino-1,3,5-triazine (PyDAT), and 6-(pyridine N-oxid-4-yl)-2,4-diamino-1,3,5-triazine (PyNODAT), synthesized in our laboratory, in addition to 6-(2,5-dichloro-phenyl)-2,4-diamino-1,3,5-triazine (DCPDAT), or irsogladine, which is a widely used anti-ulcer drug, were investigated for potential chemopreventive effects in a rat liver medium-term bioassay system. A significant inhibitory influence on enzyme-altered liver foci was found for 2CPDAT, 3CPDAT, 4CPDAT, and PyNODAT, but not for DCPDAT or PyDAT. The involvement of gap junctional intercellular communication in the inhibition was studied, but no change in gap junctional intercellular communication capacity in rat liver cells in vitro or in gap junction protein (connexin 32) expression in rat liver in vivo was noted. These results indicate that, although these irsogladine analogues exert inhibitory effects on rat liver carcinogenesis, their action is independent of modification of gap junctional intercellular communication.
The Seamstress Library (2007)

Copyright c ⃝ 2007 Hayden Walles. Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled “GNU Free Documentation License”.
Ledipasvir and sofosbuvir in patients with genotype 1 hepatitis C virus infection and compensated cirrhosis: An integrated safety and efficacy analysis (2015)

This analysis suggests that 12 weeks of LDV-SOF is safe and effective for treatment-naïve patients with HCV genotype 1 and compensated cirrhosis. The relatively lower SVR in treatment-experienced patients treated with 12 weeks of LDV-SOF raises the question of whether these patients would benefit from adding RBV or extending treatment duration to 24 weeks.
Infrared detection of hydrogen-generated free carriers in polycrystalline ZnO thin films (2005)

The changes in the free-carrier concentration in polycrystalline ZnO films during exposure to H2 and O2 plasmas were studied using in situ attenuated total reflection Fourier transform infrared spectroscopy. The carrier concentration and mobility were extracted from the free-carrier absorption in the infrared using a model for the dielectric function. The electron density in polycrystalline zinc oxide films may be significantly increased by &gt;1019cm−3 by brief exposures to hydrogen plasma at room temperature and decreased by exposure to O2 plasmas. Room-temperature oxygen plasma removes a fraction of the H at donor sites but both elevated temperatures (∼225°C) and O2 plasma were required to remove the rest. We demonstrate that combinations of O2 and H2 plasma treatments can be used to manipulate the carrier density in ZnO films. However, we also show the existence of significant drifts (∼15%) in the carrier concentrations over very long time scales (hours). Possible sites for H incorporation in polycrystalline films and reasons for the observed carrier-concentration changes are proposed.
Tricyclo[5.1.0.03.5]octan-2-ols (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTricyclo[5.1.0.03.5]octan-2-olsJoseph B. Lambert, Fred R. Koeng, and J. Warren HamersmaCite this: J. Org. Chem. 1971, 36, 20, 2941–2947Publication Date (Print):October 1, 1971Publication History Published online1 May 2002Published inissue 1 October 1971https://pubs.acs.org/doi/10.1021/jo00819a007https://doi.org/10.1021/jo00819a007research-articleACS PublicationsRequest reuse permissionsArticle Views171Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
MUC3 Human Intestinal Mucin (1997)

MUC3 is a large mucin glycoprotein expressed by the human intestine and gall bladder. In this manuscript, we present details of the deduced protein structure of MUC3. The MUC3 carboxyl-terminal domain is 617 residues in length, including 511 residues of a non-repetitive mucin-like domain (27% Thr, 22% Ser, and 11% Pro) and a 106-residue Cys-rich domain with homology to the epidermal growth factor (EGF) -like structural motifs found in many proteins. The region of MUC3 located upstream of the previously described 51-base pair (bp) tandem repeats, which encode a major Ser and Thr-rich domain, consists of a second type of repetitive structure with an imperfect periodicity of approximately 1125 bp. This domain is also mucin-like and appears to be considerably larger than 2000 residues (6000 bp). The MUC3 gene itself is large and complex. Using pulse field gel electrophoresis and blot analysis, the smallest fragment found that contained all human genomic DNA hybridizing to the 51-bp tandem repeat probe was 200 kilobases with restriction enzyme SwaI. Both PvuII and PstI produced two sets of hybridizing fragments that were hypervariable within the human population with a pattern suggestive of both a variation in the number of tandem repeats (VNTR) and sequence polymorphism. These fragments varied independently of each other, but no genetic recombination was detected in a study of 40 human families. Thus, the MUC3 gene encodes a very large glycoprotein with a structure very different from that of any mucin currently described.
Transcription on lampbrush chromosome loops in the absence of U2 snRNA. (1992)

The five small nuclear RNAs (snRNAs) involved in splicing occur on the loops of amphibian lampbrush chromosomes and in hundreds to thousands of extrachromosomal granules called B snurposomes. To assess the role of these snRNAs during transcription and to explore possible relationships between the loops and B snurposomes, we injected single-stranded antisense oligodeoxynucleotides (oligos) against U1 and U2 snRNA into toad and newt oocytes. As shown before, antisense U1 and U2 oligos caused truncation of U1 and complete destruction of U2 snRNAs, respectively. However, injection of any oligo, regardless of sequence, brought on dramatic cytological changes, including shortening of the chromosomes and retraction of the lateral loops, with concomitant shutdown of polymerase II transcription, as well as disappearance of some or all of the B snurposomes. When injected oocytes were incubated for 12 h or longer in physiological saline, these changes were reversible; that is, the chromosomes lengthened, transcription (detected by 3H-UTP incorporation) resumed on newly extended lateral loops, and B snurposomes reappeared. In situ hybridization showed that loops and B snurposomes had negligible amounts of U2 snRNA after recovery from injection of the anti-U2 oligo, whereas these structures had normal levels of U2 snRNA after recovery from a control oligo. Thus, the morphological integrity of B snurposomes and lampbrush chromosome loops is not dependent on the presence of U2 snRNA. Because transcription occurs in the absence of U2 snRNA, we conclude that splicing is not required for transcription on lampbrush chromosome loops.
A Fast Approach for Overcomplete Sparse Decomposition Based on Smoothed $\ell ^{0}$ Norm (2008)

In this paper, a fast algorithm for overcomplete sparse decomposition, called SL0, is proposed. The algorithm is essentially a method for obtaining sparse solutions of underdetermined systems of linear equations, and its applications include underdetermined sparse component analysis (SCA), atomic decomposition on overcomplete dictionaries, compressed sensing, and decoding real field codes. Contrary to previous methods, which usually solve this problem by minimizing the <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">l</i> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup> norm using linear programming (LP) techniques, our algorithm tries to directly minimize the <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">l</i> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup> norm. It is experimentally shown that the proposed algorithm is about two to three orders of magnitude faster than the state-of-the-art interior-point LP solvers, while providing the same (or better) accuracy.
Phosphotriester formation by the haloethylnitrosoureas and repair of these lesions by<i>E.coli</i>BS21 extracts (1988)

The alkylation of phosphates in DNA by therapeutically active haloethylnitrosoureas was studied by reacting N-chloroethyl-N-nitrosourea (CNU) with dTpdT, separating the products by HPLC, and identifying them by co-chromatography with authentic markers. Both hydroxyethyl and chloroethyl phosphotriesters of dTpdT were identified; a similar reaction between CNU and dTR yielded 3-hydroxyethyl and 3-chloroethyl dTR as the major products of ring alkylation. A DNA-like substrate for repair studies was synthesized by reacting 14C-labelled N-(2-chloroethyl)-N'-cyclohexyl-N-nitrosourea (14C-CCNU) with poly dT and annealing the product to poly dA. An extract of E. coli strain BS21 selectively transferred a chloroethyl group from one of the chloroethyl phosphotriester isomers in this substrate to the bacterial protein; chemical instability of the hydroxyethyl phosphotriesters precluded definite conclusions about the repair of this product.
Size- and Composition-Dependent Enhancement of Electrocatalytic Oxygen Reduction Performance in Ultrathin Palladium–Gold (Pd<sub>1–<i>x</i></sub>Au<sub><i>x</i></sub>) Nanowires (2012)

In this report, we examine the composition- and size-dependent performance in hierarchical Pd1–xAux nanowires (NWs) encapsulated with a conformal Pt monolayer shell (Pt∼Pd1–xAux). The ultrathin Pd1–xAux NWs are prepared by a solution-based method wherein the chemical composition can be readily and predictably controlled. Importantly, as-prepared Pd9Au NWs maintain significantly enhanced oxygen reduction reaction (ORR) activity (0.40 mA/cm2), as compared with elemental Pd NW/C (0.12 mA/cm2) and Pt nanoparticles (NP)/C (0.20 mA/cm2), respectively. After the deposition of a Pt monolayer, a volcano-type composition dependence is observed in the ORR activity of the Pt∼Pd1–xAux NWs as the Au content is increased from 0 to 30% with the activity of the Pt∼Pd9Au NWs (0.98 mA/cm2, 2.54 A/mgPt), representing the optimum performance. We note that the platinum group metal activity of the ultrathin 2 nm NWs (0.64 A/mg) is significantly enhanced as compared with that of analogous 50 nm NWs (0.16 A/mg) and commercial Pt NP/C (0.1–0.2 A/mg), thereby highlighting a distinctive size-dependent enhancement in NW performance.
The hypocretin/orexin system in sleep disorders: preclinical insights and clinical progress (2016)

Much of the understanding of the hypocretin/orexin (HCRT/OX) system in sleep-wake regulation came from narcolepsy-cataplexy research. The neuropeptides hypocretin-1 and -2/orexin-A and -B (HCRT-1 and -2/OX-A and -B, respectively), as we know, are intimately involved in the regulation wakefulness. The HCRT/OX system regulates sleep-wake control through complex interactions between monoaminergic/cholinergic (wake-promoting) and gamma-aminobutyric acid-ergic (sleep-promoting) neuronal systems. Deficiency of HCRT/OX results in loss of sleep-wake control or stability with consequent unstable transitions between wakefulness to nonrapid eye movement and rapid eye movement sleep. This manifests clinically as abnormal daytime sleepiness with sleep attacks and cataplexy. Research on the development of HCRT/OX agonists and antagonists for the treatment of sleep disorders has dramatically increased with the US Food and Drug Administration approval of the first-in-class dual HCRT/OX receptor antagonist for the treatment of insomnia. This review focuses on the origin, mechanisms of HCRT/OX receptors, clinical progress, and applications for the treatment of sleep disorders.
Hydraulic system design for full hybrid transmission (2011)

The design method of hydraulic system of power split full hybrid transmission is studied. Oil volume flow and pressure requirement is confirmed according to the vehicle control strategy. Mechanical gear pump and electric pump are used to supply oil flow for all driving modes. Self-adapted pressure regulation circuit is designed to supply high pressure oil for brake engagement. Dynamic model of hydraulic system was established to investigate the characteristics of oil flow and pressure. The simulation results indicate the hydraulic proposal is operative and the oil flow and pressure reach the design requirement.
Computer-assisted knee arthroplasty <i>versus</i> a conventional jig-based technique (2004)

We have compared a new technique of computer-assisted knee arthroplasty with the current conventional jig-based technique in 70 patients randomly allocated to receive either of the methods. Post-operative CT was performed according to the Perth CT Knee Arthroplasty protocol and pre- and post-operative Maquet views of the limb were taken. Intra-operative and peri-operative morbidity data were collected and blood loss measured. Post-operative CT showed a significant improvement in the alignment of the components using computer-assisted surgery in regard to femoral varus/valgus (p = 0.032), femoral rotation (p = 0.001), tibial varus/valgus (p = 0.047) tibial posterior slope (p = 0.0001), tibial rotation (p = 0.011) and femorotibial mismatch (p = 0.037). Standing alignment was also improved (p = 0.004) and blood loss was less (p = 0.0001). Computer-assisted surgery took longer with a mean increase of 13 minutes (p = 0.0001).
Matching Local Self-Similarities across Images and Videos (2007)

We present an approach for measuring similarity between visual entities (images or videos) based on matching internal self-similarities. What is correlated across images (or across video sequences) is the internal layout of local self-similarities (up to some distortions), even though the patterns generating those local self-similarities are quite different in each of the images/videos. These internal self-similarities are efficiently captured by a compact local "self-similarity descriptor"', measured densely throughout the image/video, at multiple scales, while accounting for local and global geometric distortions. This gives rise to matching capabilities of complex visual data, including detection of objects in real cluttered images using only rough hand-sketches, handling textured objects with no clear boundaries, and detecting complex actions in cluttered video data with no prior learning. We compare our measure to commonly used image-based and video-based similarity measures, and demonstrate its applicability to object detection, retrieval, and action detection.
Genome Sequence of <i>Pantoea ananatis</i> LMG20103, the Causative Agent of <i>Eucalyptus</i> Blight and Dieback (2010)

Pantoea ananatis is a Gram-negative plant pathogen that causes disease on a broad range of host plants, including pineapple, maize, rice, onion, melons, and Eucalyptus, and has been implicated in several cases of human disease. Here, we report the genome sequence of P. ananatis LMG20103 isolated from diseased Eucalyptus in South Africa.
A METHOD TO EVALUATE STRESS AND DEFORMATION OF SMALL-SIZED BURIED PIPE INDUCED BY WHEEL LOADS (1994)

都市部において使用される頻度の高く, 比較的浅層に埋設される機会の多い, 断面形状が円形で小口径の管を対象として, 一連の実験ならびに解析を実施し, その結果に基づき道路下の浅層に埋設した各種の管の応力, 変形を簡便に評価し得る方法を提案した. 更に, 実規模載荷実験による実測値と提案方法による計算値を比較したところ, バラツキはあるものの, ある程度満足すべき対比結果が得られた.
Class systems and the enforcement of social norms (1998)

We analyze a model in which there is socially inefficient competition among people. In this model, self-enforcing social norms can potentially control the inefficient competition. However, the inefficient behavior often cannot be suppressed in equilibrium among those with the lowest income due to the ineffectiveness of sanctions against those in the society with the least to lose. We demonstrate that, in such cases, it may be possible for society to be divided into distinct classes, with inefficient behavior suppressed in the upper classes but not in the lower.
Sonography of bladder and perivesical abnormalities (1981)

Sonography of bladder and perivesical abnormalitiesRL Bree and TM SilverAudio Available | Share
Strategies for the Fault-Tolerant Current Control of a Five-Phase Permanent-Magnet Motor (2007)

This paper deals with the postfault current control strategies of a five-phase permanent-magnet (PM) motor. The analysis covers both the open circuit of one and two phases and the short circuit at the machine terminal of one phase. The proposed control guarantees safe drive operation after any fault occurrence. For the sake of generality, an analytical model has been used to investigate the properties of each postfault strategy. The results are general, and they apply to PM motor of any power rating. Simulations and experimental results validate the theoretical predictions.
Heuristic functions for multiple activities with resource constraints (1993)

In this paper, we consider a class of problems in which activities compete for limited resources, and in which, once resources are assigned to activities, or groups of activities, the resulting optimization problems are relatively easy to solve. A class of heuristic functions is examined with respect to its worst-case performance ratios.
Functional Analysis of <i>Pid3-A4</i>, an Ortholog of Rice Blast Resistance Gene <i>Pid3</i> Revealed by Allele Mining in Common Wild Rice (2013)

The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice 'Digu' (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, '9311' (indica) and 'Nipponbare' (japonica). In this study, we performed functional analysis of Pid3-A4, an ortholog of Pid3 revealed by allele mining in the common wild rice A4 (Oryza rufipogon). The predicted protein encoded by Pid3-A4 shares 99.03% sequence identity with Pid3, with only nine amino-acid substitutions. In wild rice plants, Pid3-A4 is constitutively expressed, and its expression is not induced by Magnaporthe oryzae isolate Zhong-10-8-14 infection. Importantly, in transgenic plants, Pid3-A4, as compared with Pid3, displays a distinct resistance spectrum to a set of M. oryzae isolates, including those that prevail in the rice fields of Sichuan Province. Therefore, Pid3-A4 should be quite useful for the breeding of rice blast resistance, especially in southwestern China.
Automated Digital Analysis of Fiber Interferograms (1987)

An automatic method for the analysis of fiber interferograms is described. The system consists of an Aus Jena interference microscope fitted with a solid state video camera, an image array processor, and a dedicated DEC microcomputer. A key factor in the speed and accuracy of the analysis is an automatic fringe tracing procedure that eliminates operator bias. The system outputs both thickness profiles and chord-averaged refractive index profiles across the fiber sample. Examples of application to high speed spun nylon and PET and Kevlar® fibers are given.
Perceptual gamut mapping on the basis of image quality and preference factors (2006)

This study has three primary aims circumventing current limitations of color reproduction technologies: firstly, to derive base-line image quality factors from both color printer experts and academic research works. Individual factors were verified by systematic experiments, secondly, to develop a perceptual gamut mapping algorithm covering the image quality and preference factors derived, thirdly, to apply the algorithm to printer driver as acting for a vendor specific perceptual intent. Algorithm of this study tried to optimization between control parameters of gamut mapping and color shifting factors of preference, e.g. skin, sky and green grass. Profile builder using this algorithm outperforms, in industrial and academic aspects, existing commercial tool and CIE recommended algorithms.