gebhart/patrepeval huggingface dataset:
Wild-type p53 epitope naturally processed and presented by an HLA-B haplotype on human breast carcinoma cells. (1999)

To broaden the clinical applicability of peptide-based immunotherapy in breast cancer, there is a need to identify further tumor-associated peptide epitopes that are specific for HLA alleles, in addition to HLA-A2. The HLA-B44 haplotype is one of the most common HLA-B haplotypes, occurring in 10-20% of the population. We performed the structural characterization of HLA class I-bound self-peptides presented by a human breast cancer cell line with a HLA-A68, A32, B40, B44 haplotype, to identify potential tumor-specific antigens. Of 13 sequenced peptides, 1 peptide had the HLA-A68 peptide binding motif and 12 peptides had the HLA-B40, B44 peptide binding motif. One of the latter peptides, FEVRVCACPG, shared 100% homology to residues 270-279 of wild-type P53 protein. Our study, thus, provides direct evidence for the natural processing and presentation of p53 epitope 270-279 by HLA-B40, B44-bearing human breast tumor cells. Epitopes spanning this region of P53 may have potential use for immunotherapy in patients expressing HLA-A2 and -B44 supertypes.
Software update: the ORCA program system, version 4.0 (2017)

This short update provides an overview of the capabilities that have been added to the ORCA electronic structure package (version 4.0) since publication of the first article in 2012. WIREs Comput Mol Sci 2018, 8:e1327. doi: 10.1002/wcms.1327 This article is categorized under: Electronic Structure Theory > Ab Initio Electronic Structure Methods Electronic Structure Theory > Density Functional Theory Software > Quantum Chemistry
Engineering Vitamin E Content: From Arabidopsis Mutant to Soy Oil (2003)

We report the identification and biotechnological utility of a plant gene encoding the tocopherol (vitamin E) biosynthetic enzyme 2-methyl-6-phytylbenzoquinol methyltransferase. This gene was identified by map-based cloning of the Arabidopsis mutation vitamin E pathway gene3-1 (vte3-1), which causes increased accumulation of δ-tocopherol and decreased γ-tocopherol in the seed. Enzyme assays of recombinant protein supported the hypothesis that At-VTE3 encodes a 2-methyl-6-phytylbenzoquinol methyltransferase. Seed-specific expression of At-VTE3 in transgenic soybean reduced seed δ-tocopherol from 20 to 2%. These results confirm that At-VTE3 protein catalyzes the methylation of 2-methyl-6-phytylbenzoquinol in planta and show the utility of this gene in altering soybean tocopherol composition. When At-VTE3 was coexpressed with At-VTE4 (γ-tocopherol methyltransferase) in soybean, the seed accumulated to >95% α-tocopherol, a dramatic change from the normal 10%, resulting in a greater than eightfold increase of α-tocopherol and an up to fivefold increase in seed vitamin E activity. These findings demonstrate the utility of a gene identified in Arabidopsis to alter the tocopherol composition of commercial seed oils, a result with both nutritional and food quality implications.
The exploration of disease-specific gene regulatory networks in esophageal carcinoma and stomach adenocarcinoma (2019)

This proposed computational framework predicted disease-related biomolecules effectively and discovered the correlation between two types of cancers, which helped develop the diagnostic and therapeutic strategies of Esophageal Carcinoma and Stomach Adenocarcinoma.
Field localization and enhanced Second-Harmonic Generation in silicon-based microcavities (2007)

High-quality amorphous Silicon Nitride (a-Si(1-x)N(x):H) Fabry-Pérot microcavities can show resonant surface Second Harmonic Generation (SHG) effect. We consider two different layouts of planar microcavities with almost identical linear reflectance and show how the structure geometry can strongly affect SHG yield. In particular, a difference of more than one order of magnitude in the SHG intensity is observed when the fundamental beam is tuned at the cavity resonance frequency. We explain this finding on the basis of a theoretical model taking into account the spatial distribution of the electric fields of the pump and harmonic frequencies inside the structure. A satisfactory matching of experimental data with the theoretical model is obtained by considering the source of second-order nonlinearity as limited to surface contributions.
Testing for Sexually Transmitted Diseases among Women of Reproductive Age: United States, 1988 (1991)

Approximately 13.3 million women aged 15-44--about 26 percent of all women of reproductive age who had ever had intercourse--were tested for sexually transmitted diseases (STDs) in the 12 months before the 1988 National Survey of Family Growth. Slightly over half of the women tested received the test as part of a family planning visit. Women who used clinics for family planning services were most likely to be tested--54 percent compared with 34 percent of women who used a private doctor for family planning and 16 percent of women who did not receive family planning services that year. The findings suggest that access to medical care, especially access to family planning clinics, has strong effects on a woman's chances of being screened for STDs, independent of her individual characteristics. The most important individual characteristic affecting the chance of being tested for an STD was race: About 47 percent of black, 27 percent of Hispanic, and 23 percent of white and other women were tested in the 12 months before the survey. Among groups with high-risk behaviors, only 34 percent of sexually experienced teenagers were screened for STDs in the past year, as were only 43 percent of women with a positive STD history and 32 percent of women with 10 or more lifetime partners.
Mediated Electron Transfer at Redox Active Monolayers. Part 4: Kinetics of Redox Enzymes Coupled With Electron Mediators (2003)

A detailed kinetic analysis of the pertinent physical processes underlying the operation of enzyme electrodes immobilized within alkane thiol self assembled monolayers is developed. These electrodes utilize a soluble mediator, which partitions into the monolayer, regenerates the active catalytic form of the enzyme and is re-oxidized at the underlying support electrode surface giving rise to a current which reflects kinetic events at the enzyme surface. Both the enzyme/substrate and enzyme mediator kinetics have been quantified fully in terms of a ping-pong mechanism for the former and Michaelis-Menten kinetics for the latter. The effect of substrate and mediator diffusion in solution have also been specifically considered and the latter processes have been shown to result in a complex expression for the reaction flux. Four limiting kinetic cases have been enumerated and simple expressions for the reaction flux in each of these rate limiting situations have been developed. Kinetic case diagrams have been presented as an aid to mechanistic diagnosis. The complicating effects of diffusive loss of reduced mediator from the enzyme layer have also been examined and the relation between the observed flux corresponding to reduced mediator oxidation at the support electrode and the substrate reaction flux in the enzyme layer have been quantified in terms of an efficiency factor. Results extracted from recently published practical realizations of immobilized monolayer enzyme systems have been discussed in the context of the proposed model analysis.
Hospital and Surgeon Variation in Complications and Repeat Surgery Following Incident Lumbar Fusion for Common Degenerative Diagnoses (2012)

To improve the safety of lumbar spinal fusion surgery, quality improvement efforts that focus on surgeons' discretionary use of operative techniques may be more effective than those that target hospitals.
RF circuits technique of dual–band transceiver IC for GSM and DCS1800 applications (1999)

This paper describes a dual band transceiver IC for small and low cost handsets applied to GSM and DCS 1800 applications. The IC fabricated in a 0.35µm SOI BiCMOS process includes a dual band receiver, transmitter, and synthesizers. The receiver contains on-chip LNAs, and low distortion mixers. The evaluation results of the LNA of GSM and DCS1800 show gain of 18.8dB, 13.5dB, NF of 1.7dB, 1.8dB, and ICP of -8.8dBm, -8.8dBm, respectively. The mixer results for GSM are gain of 9.2dB, NF of 8.9 dB and ICP of -4.5 dBm. The mixer results for DCS1800 are gain of 10.8 dB, NF of 8.7 dB and ICP of -3.8 dBm. The reference sensitivity of -107.8 dBm for GSM and -108.3 dBm for DCS 1800 were obtained.
Selective brain cooling in humans: “fancy” or fact? (1993)

A mechanism that selectively cools the brain during hyperthermia is a well-accepted fact in animals. Selective brain cooling (SBC) during hyperthermia has also been proposed in humans, but this suggestion has met with considerable debate. Several authors have rejected the idea of human SBC for the following reasons: 1) SBC is illogical because this mechanism removes the error signal activating the defense against hyperthermia; 2) unlike other animals, humans do not pant and thus do not possess a powerful heat sink at a short distance from the brain; 3) humans do not have a carotid rete, the countercurrent heat exchanger between the arterial and venous bloods flowing in and out of the brain; 4) the high and constant arterial blood flow of the brain is sufficient to cool the brain under all conditions; and 5) the relatively low tympanic temperature (Tty) recorded in hyperthermic humans is not a sign of SBC, but rather is the sign of contamination of Tty by a low head skin temperature. These arguments are reviewed and rejected and results of several recent experiments are summarized. Finally, recent experimental articles that contradict the existence of human SBC or the validity of Tty are discussed and their conclusions refuted. This review points to overwhelming evidence in favor of human SBC.
Peroxide Desulfurization of Thioureas (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPeroxide Desulfurization of ThioureasMAX J. KALMCite this: J. Org. Chem. 1961, 26, 8, 2925–2929Publication Date (Print):August 1, 1961Publication History Published online1 May 2002Published inissue 1 August 1961https://pubs.acs.org/doi/10.1021/jo01066a072https://doi.org/10.1021/jo01066a072research-articleACS PublicationsRequest reuse permissionsArticle Views321Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Development of Social Attachments in Infancy (1964)

H. Rudolph Schaffer, Peggy E. Emerson, The Development of Social Attachments in Infancy, Monographs of the Society for Research in Child Development, Vol. 29, No. 3, The Development of Social Attachments in Infancy (1964), pp. 1+3+5-9+11-19+21-41+43-61+63-73+75-77
Ultraviolet radiation-induced melanogenesis in human melanocytes Effects of modulating protein kinase C (1994)

The mechanism by which ultraviolet radiation induces melanogenesis in epidermal melanocytes is unknown. Previous observations that in cultured human melanocytes 1-oleoyl-2-acetylglycerol augmented both basal and ultraviolet radiation-induced melanogenesis, suggested that the responses were mediated via protein kinase C. However, paradoxically the phorbol ester TPA was without effect. Therefore, the present study has examined the involvement of protein kinase C in melanogenesis. Analysis of the isozyme profile of human melanocytes revealed the presence of protein kinase C alpha, beta I, epsilon and zeta but not the isozyme eta. Following exposure to 500 nM TPA for 24 hours, isozymes alpha, beta I and epsilon were downregulated, but zeta was unaffected. Similar isozyme profiles were observed in S91 and SKMEL3 melanoma cells. The melanogenic responses to 1-oleoyl-2-acetylglycerol and ultraviolet radiation were unaffected by inhibition of protein kinase C with Ro31-8220, or ablation by downregulation with 500 nM TPA, in human melanocytes and melanoma cells. 1-Oleoyl-2-acetylglycerol had no effect on protein kinase C activity in human melanocytes, as measured by rapid phosphorylation of the 80 kDa protein myristoylated alanine-rich C kinase substrate (MARCKS). Ultraviolet radiation induced a small increase in MARCKS protein phosphorylation but this effect was inhibited by pretreatment for 24 hours with 500 nM TPA, which had no effect on ultraviolet-induced melanogenesis. Overall, these findings indicate that 1-oleoyl-2-acetylglycerol and ultraviolet radiation activate melanogenesis via protein kinase C-independent pathways.
Teaching of Electronic Technology (2012)

Electronic technic training are an important foundation course for the system of engineering training.It is plays a very important role in improve students ' ability to innovate,and analysis and solve practical problems.The article according to the experience of many years of teaching,summed up for this course content and teaching methods to make the teaching more in line with the actual teaching and development.
Complete sequence analysis of a gene (OS-9) ubiquitously expressed in human tissues and amplified in sarcomas (1996)

Amplification and overexpression of genes involved in cellular growth control occur frequently in human tumors. Using a chromosome microdissection-based hybrid-selection strategy, we recently identified two novel genes (OS-9 and OS-4) within 12q13-15, a region frequently amplified in human cancers. We now report further characterization of the full-length OS-9 cDNA sequence consists of 2785 bp from which an open reading frame (ORF) with 667 amino-acid residues was deduced, The predicted polypeptide was water soluble and acidic. We also demonstrate that the OS-9 gene encoded a 2.8-kb mRNA transcribed in all 16 human tissues examined, suggesting that OS-9 is ubiquitously expressed in human tissues. OS-9 was co-amplified with CDK4 in three of five sarcoma tissues. Homology analysis of the amino-acid sequence reveals significant similarities between OS-9 and two ORFs deduced from genomic sequences in Caenorhabditis elegans and Saccharomyces cerevisiae. The region of similarity extended over 200 residues (approximately one-third of each ORF), and eight cysteines were conserved in all three ORFs. These observations suggest that this region comprises a functional domain present in a novel evolutionarily conserved gene family defined by OS-9.
Conjugated Polymers via Electrochemical Polymerization of Thieno[3,4-<i>b</i>]thiophene (T34bT) and 3,4-Ethylenedioxythiophene (EDOT) (2003)

Herein the electrochemical polymerizations of thieno[3,4-b]thiophene (T34bT) and 3,4-ethylenedioxythiophene (EDOT) and the electrochemical copolymerizations of T34bT and EDOT carried out in tetrabutylammonium perchlorate and tetrabutylammonium hexafluorophosphate electrolyte are compared utilizing cyclic voltammetry, chronocoulometry, and chronogravimetry. Simultaneous polymerization of T34bT and EDOT produced a copolymer, as confirmed by vis-NIR spectroscopy. The simultaneous polymerization of T34bT and EDOT exhibits a peak for monomer oxidation intermediate to the monomer oxidation peaks for each of the single component monomer systems. Furthermore, the copolymer exhibited tremendous stability to n-doping in comparison to either of the homopolymers of T34bT or EDOT. Polymerization rates as determined via chronogravimetry were found to be highest for EDOT with the lowest rate being obtained for the copolymerization of T34bT and EDOT. All polymers exhibited high anion dominancy (>80%) with the highest value, ∼92%, exhibited by PT34bT. Doping levels ranging from 27 to 38% were obtained for polymers containing T34bT. PEDOT was determined to exhibit doping levels of approximately 15%.
Revascularization of the heart by laser (1981)

The possibility of revascularizing the myocardium with high-energy laser was investigated based on the finding that the laser beam could produce small channels in the tissue of the myocardium that were devoid of debris and scarring. The technique was investigated in 4 groups of 6 mongrel dogs each. In 3 of the groups, the left anterior descending (LAD) coronary artery was ligated above the first diagonal branch and the myocardium was treated with the laser energy according to the protocol of that group. In the fourth control group, the LAD artery was ligated but the myocardium was not subjected to laser treatment. In the first 3 groups, the myocardium was protected by the channels produced by the application of the laser energy. The animals were subsequently sacrificed at various intervals. In the control group, all of the animals died within 20 minutes of ligation of the artery. At autopsy, the epicardial sites of the channels created by the laser were clearly visible and a lack of charring in the channels was demonstrated. The penetration through the endocardial surface could be demonstrated. Microscopically, the channels were patent and endothelialized. It is concluded that the channels created in the myocardium effectively helped to protect the myocardium from acute coronary artery occlusion in this model in dogs.
Modulation of Substrate Specificity upon Modification of Cholesterol Oxidase from <i>Brevibacterium Sterolicum</i> by Hydrogen Peroxide: Evidence for a Peripheral Site (2004)

Abstract Evidence for a peripheral site that recognizes micelles where cholesterol is inserted, is presented for cholesterol oxidase from Brevibacterium sterolicum (CHOD). The Ω loop part of this site contains methionine residues. Their oxidation is reflected in a different selectivity to the micelles. When CHOD is oxidized with hydrogen peroxide, preferential introduction of two oxygen atoms by hydrogen peroxide to the enzyme is observed by the electrospray mass spectrometry. The modified enzyme does not oxidize cholesterol inserted in H‐Triton X‐100/sodium cholate mixed micelles. However, cholesterol solubilized in microemulsions, in biphasic media or inserted in hydroxypropyl‐β‐cyclodextrin is oxidized by the modified enzyme. This modified enzyme oxidizes pregn‐5‐en‐3β‐ol solubilized in mixed micelles, and soluble steroids, such as 3β‐hydroxyandrost‐5‐en‐17β‐carboxylic acid or 3β‐hydroxyandrost‐5‐en‐17‐one. So the modification does not occur at the active site, but at the peripheral site (methionine 81), abolishing the recognition of the micelles and thus inactivating the enzyme specifically towards cholesterol inserted in the micelles.
Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR (1994)

Several PCR assays which identify the genus Brucella but do not discriminate among species have been reported. We describe a PCR assay that comprises five oligonucleotide primers which can identify selected biovars of four species of Brucella. Individual biovars within a species are not differentiated. The assay can identify three biovars (1, 2, and 4) of B. abortus, all three biovars of B. melitensis, biovar 1 of B. suis, and all B. ovis biovars. These biovars include all of the Brucella species typically isolated from cattle in the United States, a goal of the present research. The assay exploits the polymorphism arising from species-specific localization of the genetic element IS711 in the Brucella chromosome. Identity is determined by the size(s) of the product(s) amplified from primers hybridizing at various distances from the element. The performance of the assay with U.S. field isolates was highly effective. When 107 field isolates were screened by the described method, there was 100% agreement with the identifications made by conventional methods. Six closely related bacteria (Agrobacterium radiobacter, Agrobacterium rhizogenes, Ochrobactrum anthropi, Rhizobium leguminosarum, Rhizobium meliloti, and Rhodospirillum rubrum) and two control bacteria (Bordetella bronchiseptica and Escherichia coli) tested negative by the assay.
12  kW clad pumped Raman all-passive-fiber laser with brightness enhancement (2018)

An all-fiber clad pumped Raman fiber laser (FL) oscillator with a CW power of 1.2 kW and an efficiency of 85% is presented. To the best of our knowledge, this laser is the highest power and the highest efficiency Raman FL demonstrated in any configuration with brightness enhancement (BE). To the best of our knowledge, it is also the first greater than kilowatt FL of any kind that does not utilize rare-earth doping in the oscillator fiber (all passive fiber). The beam quality (BQ) of the Raman laser was M<sup>2</sup>=2.75 at 1 kW, and the pump-Stokes BE factor was approximately 7. The laser consists of a specially designed triple-clad fiber which confines the low BQ pump power into the multi-mode inner clad, while generating the Raman signal in the large-mode-area core. The second Stokes is inhibited by selecting the appropriate inner clad-to-core area ratio and by the oscillator's selective fiber Bragg grating reflectors.
Tokamak experimental power reactor studies (1975)

Three designs of an EPR are briefly described. The plasma physics involved in the EPR design is discussed. Brief discussions on the following topics are included: (1) MHD equilibrium, (2) impurity control, (3) transport scaling, (4) burn cycle dynamics, (5) first wall system, (6) blanket/shield systems, (7) plasma heating, (8) toroidal field coils, (9) poloidal field coils, (10) energy storage and transfer, (11) tritium fuel cycle, (12) radiation damage, and (13) costs. (MOW)
Doxycycline versus doxycycline and rifampin in undifferentiated spondyloarthropathy, with special reference to chlamydia-induced arthritis. A prospective, randomized 9-month comparison. (2004)

The combination of doxycycline and rifampin for 9 months seemed to be effective in treatment of chronic uSpA. This is the first study to demonstrate therapeutic benefit with antimicrobials to a chronic inflammatory arthritis possibly secondary to persistent Chlamydia.