gebhart/patrepeval huggingface dataset:
The characterization of a murine model of mucopolysaccharidosis II (Hunter syndrome) (2007)

Mucopolysaccharidosis II (MPS II, Hunter syndrome in humans) is an X-linked inherited lysosomal storage disease caused by a deficiency in the lysosomal enzyme iduronate-2-sulfatase (I2S). I2S catalyses a step in the catabolism of glycosaminoglycans (GAGs) dermatan sulfate and heparan sulfate, and when it is deficient or absent GAGs accumulate in tissues and organs. Male knockout mice (IdS-KO), which lack the gene coding for I2S, exhibit many of the characteristics seen in the human disease. Compared to wild-type control mice, urine GAG excretion was elevated at 4 weeks of age and remained high throughout the lifespan, and tissue GAG levels were elevated as early as 7 weeks of age. Liver, spleen and other organs were significantly larger in the IdS-KO mice than in the wild-type. Radiographic examination revealed sclerosis and enlargement of the skull at 4 weeks of age and appendicular bone enlargement at 10-13 weeks of age. Micro CT scans showed severe periosteal bone formation at the lateral aspect of the distal tibia and calcification of the calcaneus tendon. This model was used in the development of idursulfase for treatment of MPS II and may continue to be useful in the evaluation of treatment strategies of this chronic and progressive disorder.
Highly crystalline MOF-based materials grown on electrospun nanofibers (2015)

Supported Metal Organic Frameworks (MOFs) with a high specific surface area are of great interest for applications in gas storage, separation, sensing, and catalysis. In the present work we report the synthesis of a novel composite architecture of MOF materials supported on a flexible mat of electrospun nanofibers. The system, based on three-dimensional interwoven nanofibers, was designed by using a low-cost and scalable multistep synthesis protocol involving a combination of electrospinning and low-temperature atomic layer deposition of oxide materials, and their subsequent solvothermal conversion under either conventional or microwave-assisted heating. This highly versatile approach allows the production of different types of supported MOF crystals with controlled sizes, morphology, orientation and high accessibility.
Zn2+-dependent reversible inactivation of rat liver phosphofructokinase-1. Purification of the inactivating protein and characterization of the inactivation reaction. (1986)

A protein has been purified from rat liver (about 5 mg from 100 g) which inactivates rat liver phosphofructokinase-1. According to dodecyl sulfate gel electrophoresis the protein consists of a single peptide chain with a Mr of 19,000. The inactivation of phosphofructokinase-1 by this protein results from a dissociation of phosphofructokinase-1 into its inactive protomers (Mr = 82,000). The inactivation is dependent on zinc ions in micromolar concentration (about 1-2 microM), but is inhibited by higher concentrations (greater than 50 microM). Fructose 1,6-bisphosphate as well as fructose 2,6-bisphosphate inhibit the inactivation reaction. In addition, both compounds as well as ATP can reverse the dissociation of phosphofructokinase-1. The phosphofructokinase-1 inactivating protein has no phosphatase activity with [32P]phosphofructokinase or low molecular weight phospho-compounds and does not possess any detectable proteolytic activity. It has the same affinity for the phospho- and the dephosphoform of phosphofructokinase-1, but preincubation of phosphofructokinase-1 with this inactivating protein reduces the maximum amount of phosphate incorporated into phosphofructokinase-1 and accelerates the velocity of the dephosphorylation reaction. A direct Zn2+-dependent binding of phosphofructokinase-1 to the inactivating protein has been demonstrated in experiments with matrix-bound phosphofructokinase-1 inactivating protein.
Differential Fluorescent Staining of Human Chromosomes with Daunomycin and Adriamycin—The D-Bands (1975)

Human chromosome preparations were treated with a group of anthracycline antibiotics. Well-defined, orange-red fluorescent bands were observed on chromosomes after the slide was stained with daunomycin and adriamycin but not with nogalamycin. The characteristic differential bands appeared to be similar to the banding patterns obtained by the quinacrine techniques. Interaction of these antibiotics with DNA could provide information on the general mechanism of fluorescent banding. Further, these bands (D-bands) appeared to be more stable than the Q-bands and may have some usefulness for routine clinical cytogenetic analysis.
Ambulatory Quantitative Assessment of Body Position, Bradykinesia, and Hypokinesia in Parkinson's Disease (1998)

We used ambulatory monitoring to quantify body position, bradykinesia, and hypokinesia simultaneously in 50 patients with Parkinson's disease (PD) and 43 healthy elderly during the diurnal period. Reliable automatic detection of three defined body positions proved possible. As compared with controls, PD patients spent less time upright and more time during the day lying down, which correlated well with the self-reported time spent lying down. PD patients had significantly lower mean values of extremity acceleration and higher mean values of immobility than controls. The objective measures of bradykinesia and hypokinesia showed only a modest or no relation to the semiquantitative subjective Unified Parkinson's Disease Rating Scale (UPDRS) motor scores, which most likely was due to differences between the methods. In contrast to bradykinesia measures, hypokinesia measures showed clear sex differences in both patients and controls. Over time, trunk and arm movements occurred more frequently in women than in men. Our ambulatory monitoring assessment disclosed clinically relevant information about the mobility profile and offers a way to quantify cardinal movement features simultaneously in PD patients throughout the day.
[Review of] John A. Grim. The Shaman: Patterns of Siberian and Ojibway Healing (1987)

John A. Grim utilizes the methodology of the fields of anthropology, mythology, psychology, and sociology to elucidate the religious meaning of shamanism as exemplified in Siberian and Ojibway societies. Although shamans have long been viewed as primordial religious personalities, a comprehensive interpretation of the shamanic religious experience has been lacking. This book provides important insights that will be of interest to scholars and general readers interested in the American Indian religious experience.
The Interest Rate Pass-Through: An Empirical Study Of Seven Asian Economies (2017)

This paper examines the extent of interest rate pass-through in seven Asian economies including Indonesia, Korea, the Philippines, Singapore, Thailand, Hong Kong, and Taiwan. The data selected ranges from after the 1997 Asian financial crisis to right before the 2008 financial turmoil and examines the question of whether these Asian economiesâ€™ banking systems have recovered and become stronger since the 1997 financial crisis. The Error Correction Model was adopted in the study, and the empirical results suggested that banks adjust the markets to their retail rates with some levels of delay and the extent of the adjustments in intermediate and long-term pass-through vary depending on the nature of financial instruments and the length of their maturities. In general, countries with more highly developed financial markets usually respond to market conditions more actively and fully, while countries with less developed financial markets tend to have a longer adjustment process. Lending rates are usually stickier than deposit rates in the countries studied
The instability of a dispersion of sedimenting spheroids (1989)

It is shown that hydrodynamic interactions between non-Brownian, non-spherical, sedimenting particles give rise to an increase in the number of neighbouring particles in the vicinity of any given particle. This result suggests that the suspension is unstable to particle density fluctuations even in the absence of inertia; a linear stability analysis confirms this inference. It is argued that the instability will lead to convection on a lengthscale ( nl ) −½ , where l is a characteristic particle length and n is the particle number density. Sedimenting suspensions of spherical particles are shown to be stable in the absence of inertial effects.
Generating reusable web components from mockups (2018)

The transformation of a user interface mockup designed by a graphic designer to web components in the final app built by a web developer is often laborious, involving manual and time consuming steps. We propose an approach to automate this aspect of web development by generating reusable web components from a mockup. Our approach employs visual analysis of the mockup, and unsupervised learning of visual cues to create reusable web components (e.g., React components). We evaluated our approach, implemented in a tool called VizMod, on five real-world web mockups, and assessed the transformations and generated components through comparison with web development experts. The results show that VizMod achieves on average 94% precision and 75% recall in terms of agreement with the developers' assessment. Furthermore, the refactorings yielded 22% code reusability, on average.
Effect of QPQ Salt Bath Oxidation on Corrosion Resistance (2006)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Chronic plasma cell endometritis in hysterectomy specimens of HIV-infected women: A retrospective analysis (1998)

Chronic endometritis was common and of a higher grade among HIV-infected women requiring hysterectomy in our series. Diagnosis and treatment of endometritis should be considered in HIV-infected women with uterine bleeding and/or tenderness. We speculate that antiretroviral and/or antimicrobial treatment for endometritis may effectively treat endometritis and eliminate the need for surgery in some HIV-infected women. We suggest that consideration and treatment of endometritis in HIV-1 infected women being evaluated for possible hysterectomy has the potential to reduce costs and morbidity for patients and providers who may be exposed during surgical procedures.
Composite Camshaft - Avoid Lobe Grinding Using Precision PM Lobes (1997)

<div class="htmlview paragraph">In order to obtain an optimum condition of valve lift running with roller followers, in most cases the lobe profile needs to have a concave form (negative radii in the area of the cam lift profile). These negative radii can be less than 50mm. Conventional grinding equipment is not suitable to manufacture this type of cam profile. To avoid these disadvantages, a new type of composite camshaft has been developed.</div> <div class="htmlview paragraph">Precision PM lobes are fitted by the thermal shrink fit process to a prepared shaft and as a result no grinding of the lobes is necessary. To avoid lobe grinding, a manufacturing process for the lobes and camshafts was developed, which produces a sufficiently accurate lobe form within the process.</div>
Solvents from Pine (1943)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolvents from PineR. C. PalmerCite this: Ind. Eng. Chem. 1943, 35, 10, 1023–1025Publication Date (Print):October 1, 1943Publication History Published online1 May 2002Published inissue 1 October 1943https://pubs.acs.org/doi/10.1021/ie50406a003https://doi.org/10.1021/ie50406a003research-articleACS PublicationsRequest reuse permissionsArticle Views84Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Peptide charge state determination for low-resolution tandem mass spectra (2005)

Mass spectrometry is a particularly useful technology for the rapid and robust identification of peptides and proteins in complex mixtures. Peptide sequences can be identified by correlating their observed tandem mass spectra (MS/MS) with theoretical spectra of peptides from a sequence database. Unfortunately, to perform this search the charge of the peptide must be known, and current chargestate- determination algorithms only discriminate singlyfrom multiply-charged spectra: distinguishing +2 from +3, for example, is unreliable. Thus, search software is forced to search multiply-charged spectra multiple times. To minimize this inefficiency, we present a support vector machine (SVM) that quickly and reliably classifies multiplycharged spectra as having either a +2 or +3 precursor peptide ion. By classifying multiply-charged spectra, we obtain a 40% reduction in search time while maintaining an average of 99% of peptide and 99% of protein identifications originally obtained from these spectra.
A generalized metric distance between hierarchically partitioned images (2005)

This article presents a generalized metric distance, called Δ-distance, between images represented by a tree structure resulting from a recursive image partition. This distance is used to perform content-based image retrieval queries in databases. Δ-distance allows to retrieve images globally similar to a query image. This distance takes into account the location of the image visual features. It can be performed using a multi-level filtering algorithm. Moreover, Δ-distance allows region-based queries. In this case, the resulting images contain quadrants similar to the quadrants selected by the user in the query image or contain quadrants similar to the entire query image. Because it is a generalized distance function, some particular cases of the Δ-distance appear in existing content-based image retrieval systems.
Highly sensitive stool DNA testing of <i>Fusobacterium nucleatum</i> as a marker for detection of colorectal tumours in a Japanese population (2016)

Background Accumulating evidence shows an over-abundance of Fusobacterium nucleatum in colorectal tumour tissues. Although stool DNA testing of Fusobacterium nucleatum might be a potential marker for the detection of colorectal tumours, the difficulty in detecting Fusobacterium nucleatum in stool by conventional methods prevented further explorations. Therefore, we developed a droplet digital polymerase chain reaction (PCR) assay for detecting Fusobacterium nucleatum in stool and investigated its clinical utility in the management of colorectal tumours in a Japanese population. Methods Feces were collected from 60 healthy subjects (control group) and from 11 patients with colorectal non-advanced adenomas (non-advanced adenoma group), 19 patients with colorectal advanced adenoma/carcinoma in situ (advanced adenoma/carcinoma in situ (CIS) group) and 158 patients with colorectal cancer of stages I to IV (colorectal cancer group). Absolute copy numbers of Fusobacterium nucleatum were measured by droplet digital PCR. Results The median copy number of Fusobacterium nucleatum was 17.5 in the control group, 311 in the non-advanced adenoma group, 122 in the advanced adenoma/CIS group, and 317 in the colorectal cancer group. In comparison with that in the control group, the Fusobacterium nucleatum level was significantly higher in the non-advanced adenoma group, the advanced adenoma/CIS group and the colorectal cancer group. Conclusions This study illustrates the potential of stool DNA testing of Fusobacterium nucleatum by droplet digital PCR to detect individuals with colorectal tumours in a Japanese population.
Targeted Identification of Sialoglycoproteins in Hypoxic Endothelial Cells and Validation in Zebrafish Reveal Roles for Proteins in Angiogenesis (2014)

The formation of new vessels in the tumor, termed angiogenesis, is essential for primary tumor growth and facilitates tumor invasion and metastasis. Hypoxia has been described as one trigger of angiogenesis. Indeed, hypoxia, which is characterized by areas of low oxygen levels, is a hallmark of solid tumors arising from an imbalance between oxygen delivery and consumption. Hypoxic conditions have profound effects on the different components of the tumoral environment. For example, hypoxia is able to activate endothelial cells, leading to angiogenesis but also thereby initiating a cascade of reactions involving neutrophils, smooth muscle cells, and fibroblasts. In addition, hypoxia directly regulates the expression of many genes for which the role and the importance in the tumoral environment remain to be completely elucidated. In this study, we used a method to selectively label sialoglycoproteins to identify new membrane and secreted proteins involved in the adaptative process of endothelial cells by mass spectrometry-based proteomics. We used an in vitro assay under hypoxic condition to observe an increase of protein expression or modifications of glycosylation. Then the function of the identified proteins was assessed in a vasculogenesis assay in vivo by using a morpholino strategy in zebrafish. First, our approach was validated by the identification of sialoglycoproteins such as CD105, neuropilin-1, and CLEC14A, which have already been described as playing key roles in angiogenesis. Second, we identified several new proteins regulated by hypoxia and demonstrated for the first time the pivotal role of GLUT-1, TMEM16F, and SDF4 in angiogenesis.
Opposing Action of Casein Kinase 1 and Calcineurin in Nucleo-cytoplasmic Shuttling of Mammalian Translation Initiation Factor eIF6 (2010)

Eukaryotic initiation factor 6 (eIF6), a highly conserved protein from yeast to mammals, is essential for 60 S ribosome biogenesis and assembly. Both yeast and mammalian eIF6 are phosphorylated at Ser-174 and Ser-175 by the nuclear isoform of casein kinase 1 (CK1). The molecular basis of eIF6 phosphorylation, however, remains elusive. In the present work, we show that subcellular distribution of eIF6 in the nuclei and the cytoplasm of mammalian cells is mediated by dephosphorylation and phosphorylation, respectively. This nucleo-cytoplasmic shuttling is dependent on the phosphorylation status at Ser-174 and Ser-175 of eIF6. We demonstrate that Ca(2+)-activated calcineurin phosphatase binds to and promotes nuclear localization of eIF6. Increase in intracellular concentration of Ca(2+) leads to rapid translocation of eIF6 from the cytoplasm to the nucleus, an event that is blocked by specific calcineurin inhibitors cyclosporin A or FK520. Nuclear export of eIF6 is regulated by phosphorylation at Ser-174 and Ser-175 by the nuclear isoform of CK1. Mutation of eIF6 at the phosphorylatable Ser-174 and Ser-175 to alanine or treatment of cells with the CK1 inhibitor, D4476 inhibits nuclear export of eIF6 and results in nuclear accumulation of eIF6. Together, these results establish eIF6 as a substrate for calcineurin and suggest a novel paradigm for calcineurin function in 60 S ribosome biogenesis via regulating the nuclear accumulation of eIF6.
Complications of Third-Trimester Amniocentesis Using Continuous Ultrasound Guidance (2002)

In Brief OBJECTIVE The objective of the study was to estimate the risks of third-trimester amniocentesis with continuous ultrasound guidance. METHODS Cohort study. We reviewed the medical records of women who had an amniocentesis with continuous ultrasound guidance after 30 weeks' gestation at a single institution from January 1991 through December 1994. For procedures performed from January 1991 to February 1994, we obtained information from a chart review. From March 1994 to December 1994, we collected data prospectively. The primary outcome was whether or not there were any complications within 48 hours of the procedure. We also sought to determine any risk factors associated with complications. RESULTS Complete records and data were available for 562 amniocenteses during the study period. The mean gestational age at the time of amniocentesis was 34.9 weeks. Of the 562 procedures, five (0.8%) were unsuccessful and 50 (9%) required more than one needle stick. The complication rate was 0.7% (95% confidence level (CI) = 0.02%, 1.9%). These included spontaneous labor in a preterm gestation (1), premature rupture of the membranes (1), placental abruption (1), and fetal–maternal hemorrhage (1). No patient required an emergency cesarean delivery and none suffered a perinatal death (95% CI 0, 0.8%). Complications were not associated with the number of needle sticks, the presence of bloody amniotic fluid, or the level of operator experience. CONCLUSIONS Third-trimester amniocentesis performed with continuous ultrasound guidance has a high success rate and low risk for complications. Third-trimester amniocentesis with continuous ultrasound guidance has a high success rate and a low complication rate.
Domain II Mutations in CRANE/IAA18 Suppress Lateral Root Formation and Affect Shoot Development in Arabidopsis thaliana (2008)

Lateral root formation is an important developmental component of root systems in vascular plants. Several regulatory genes for lateral root formation have been identified from recent studies mainly using Arabidopsis thaliana. In this study, we isolated two dominant mutant alleles, crane-1 and crane-2, which are defective in lateral root formation in Arabidopsis. The crane mutants have dramatically reduced lateral root and auxin-induced lateral root formation, indicating that the crane mutations reduce auxin sensitivity. In addition, the crane mutants have pleiotropic phenotypes in the aerial shoots, including long hypocotyls when grown in the light, up-curled leaves and reduced fertility. The crane mutant phenotypes are caused by a gain-of-function mutation in domain II of IAA18, a member of the Aux/IAA transcriptional repressor family which is expressed in almost all organs. In roots, IAA18 promoter::GUS was expressed in the early stages of lateral root development. In the yeast two-hybrid system, IAA18 interacts with AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19, transcriptional activators that positively regulate lateral root formation. Taken together, our results indicate that CRANE/IAA18 is involved in lateral root formation in Arabidopsis, and suggest that it negatively regulates the activity of ARF7 and ARF19 for lateral root formation.