gebhart/patrepeval huggingface dataset:
BRCA1‐IRIS activates cyclin D1 expression in breast cancer cells by downregulating the JNK phosphatase DUSP3/VHR (2007)

Cyclin D1 plays an important role in cell cycle progression. In breast cancer, Cyclin D1 expression is deregulated by several mechanisms. We previously showed that in breast cancer cells, overexpression of BRCA1-IRIS induces Cyclin D1 overexpression and increases cell proliferation. BRCA1-IRIS alone or in complex with steroid receptor co-activators was targeted to the cyclin D1 promoter pre-bound by the c-Jun/AP1 and activated its transcription, which could explain the co-overexpression of BRCA1-IRIS and Cyclin D1 in breast cancer cells coupled with their increased proliferation. We report here an alternate or a complementary pathway by which BRCA1-IRIS activates Cyclin D1 expression. BRCA1-IRIS overexpression decreases the expression of the dual specificity phosphatase, DUSP3/VHR, an endogenous inhibitor of several MAPKs, including c-Jun N-terminal kinase. Although, the mechanism by which BRCA1-IRIS overexpression accomplishes that is not yet known, it is sufficient to induce Cyclin D1 overexpression in a human mammary epithelial cell model. Cyclin D1 overexpression could be blocked by co-overexpression of VHR in those cells. Furthermore, in 2 breast cancer cell lines that overexpress both BRCA1-IRIS and Cyclin D1 (MCF-7 and SKBR3) depletion of BRCA1-IRIS by RNA interference attenuated the expression of Cyclin D1 by elevating the expression level of VHR. These data demonstrate a critical role for BRCA1-IRIS in human breast cancer cell-cycle control and suggest that deregulated expression of BRCA1-IRIS is likely to reduce dependence on normal physiological growth stimuli, thereby providing a growth advantage to tumor cells and a potential mechanism of resistance to endocrine therapy.
Low Noise Integrated SHF Converter for Satellite TV Broadcasting (1986)

We have successfully developed a high performance SHF converter for satellite TV broadcasting system, which converts the 12GHz incoming signal down to the 1GHz signal.
An Overview of Real-Time Operating Systems (2007)

Over the past 25 years, advances in semiconductor manufacturing have led to smaller and faster computers, which in turn has stimulated the development of “smarter” laboratory devices that can control complex networks of devices and process large amounts of data fast and reliably. As more functionality is pushed down to laboratory devices and personal computers, the sophistication needed to manage external resources, events, and information grows. In some cases, only realtime operating systems (RTOSs) can meet the time and resource constraints of such systems. Whether you write your own software for lab automation, write middleware to help communicate between programs, or use off-the-shelf software, it is beneficial to know when a RTOS is an appropriate platform for your application. This paper provides an overview of RTOSs, the criteria needed for their evaluation, and examples of typical RTOSs. Our main purpose is to enable the reader to understand basic concepts of real-time systems and to stimulate further investigation into their unique properties in the context of laboratory automation.
5-Arylideneaminouracils: II. Synthesis of sodium and ammonium salts (2009)

In continuation of studies on the design of new 5-arylideneaminouracil derivatives possessing antimicrobial and antiviral properties, the corresponding ammonium and sodium salts were synthesized. The antimicrobial activity of water-soluble salts of substituted aminouracils in vitro was shown to be several times higher than that of parent neutral compounds.
Demand Assigned Multiple Access Systems Using Collision Type Request Channels: Traffic Capacity Comparisons (1979)

The traffic carrying capabilities of demand assigned multiple access (DAMA) systems in which requests for communication circuits are transmitted over shared collision type channels to a master controller are investigated. The circuit request protocol permits both busy and blocked responses in addition to circuit allocations. The theoretically maximum traffic capacity is determined and it is shown that even very simple systems of this type can achieve efficient bandwidth utilization in realistic situations.
Millimeter-Wave Electronically Steerable Integrated Lens Antennas for WLAN/WPAN Applications (2012)

This paper presents design and experimental verification of electronically steerable integrated lens antennas (ILAs) for WLAN/WPAN communication systems operating in the 60-GHz frequency band. The antenna is comprised of a quartz extended hemispherical lens, four switched aperture coupled microstrip antenna (ACMA) elements, and a distribution circuit based on SPDT MMIC switches. The designed ILAs are capable of electronic steering between four different antenna main beam directions in one plane. Fixed beam and electronically steerable ILA prototypes are fabricated and tested. The results are given for two quartz dielectric lenses with the radii of 7.5 and 12.5 mm in order to meet a wide range of WLAN/WPAN requirements. The measured maximum gains of the designed ILAs are 18.4 and 23.2 dBi. The experimental results of the fabricated electronically steerable quartz ILA prototypes prove the simulation results and show ±35 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">°</sup> and ±22 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">°</sup> angle sector coverage for the lenses with the 7.5 and 12.5 mm radii, respectively. The bandwidth of the ILAs exceeds the frequency band of 57-66 GHz allocated for WLAN/WPAN applications. The designed ILAs meet all the requirements for steerable directional antennas of 60-GHz WLAN/WPAN systems.
Cryptosporidium merozoite isolation and purification using differential centrifugation techniques. (1992)

Simple modifications to a recently published merozoite purification procedure (Bjorneby et al., J. Immunol. 145:298, 1990) increased yields 3- to 5-fold. Calves were infected with 2.5 x 10(8) Cryptosporidium parvum oocysts and sacrificed 65 h post-infection. The ilium and caecum were removed. The tissue was sieved through a large strainer (2 mm2) to produce a homogeneous suspension. Red blood cells were removed by differential centrifugation (600 g); merozoites remained in the supernatant. The merozoites were pelleted (2,100 g) and washed in modified Hank's balanced salt solution deficient in Mg+2 and Ca+2. Percoll purification (density 1.070 g/ml and centrifugation speed of 22,000 g for 30 min) yielded 8 x 10(8) merozoites. Nineteen monoclonal antibodies (MAb) detected by either an enzyme-linked immunosorbent assay or an immunofluorescence assay, have been generated against the merozoite stage. Gels of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver-stained showed that sporozoites and merozoites have many common lower molecular weight proteins. Western blots of sporozoite and merozoite antigens reacted with anti-sporozoite MAb showed several cross-reacting antigens shared by these life-cycle stages.
New insights into the composition, molecular mass and stoichiometry of the protein complexes of plant mitochondria (1996)

Recently a powerful electrophoresis method for the native preparation and characterization of the respiratory protein complexes of mitochondria from fungi and mammals has been developed, which employs Coomassie dyes to introduce charge shifts on proteins (Schägger and von Jagow (1991) Anal. Biochem. 199, 223-231). The procedure, which is called 'blue native-polyacrylamide gel electrophoresis' (BN-PAGE), was modified and introduced for the analysis of mitochondria from higher plants. BN-PAGE of mitochondrial protein from potato allows the separation of nine distinct protein complexes between 100 and 1000 kDa and reveals novel results for their composition, molecular mass and stoichiometry. For the first time soluble mitochondrial protein complexes, like the HSP60 complex (750 kDa) and a complex of 200 kDa, which includes a formate dehydrogenase, are analysed by BN-PAGE. Complex I from potato (1000 kDa) is about 100 kDa larger than the corresponding enzyme from beef and can be resolved into more than 30 different subunits on a second gel dimension. The F1F0 ATP synthase (580 kDa) and the cytochrome c oxidase (160 kDa) from potato seem to contain more subunits than hitherto reported. Direct sequencing of subunits revealed that the F1 part of the F1F0 ATP synthase lacks the oligomycin sensitivity conferring protein (OSCP), which was reported to be present in F1 parts of dicotyledonous plants, but contains the ATPase inhibitory protein. N-terminal sequences of 16 mitochondrial proteins were obtained, several of which are presented for the first time from a plant source. BN-PAGE allows the preparation of mitochondrial protein complexes from gram amounts of plant tissue, as the procedure only requires milligram amounts of organelles. This potential of BN-PAGE is demonstrated by the separation and characterization of the mitochondrial enzyme complexes from Arabidopsis thaliana. Further analysis of organellar protein complexes by BN-PAGE will allow the generation of 'protein maps' from different tissues and developmental stages or from mutant plants.
A WDMA protocol for multichannel DQDB networks (2002)

Wavelength division multiplexing (WDM) provides another dimension of freedom to tap into the vast optical bandwidth. High-speed lightwave networks employing linear fiber bus topology have become attractive with the emergence of erbium-doped optical amplifiers. In this paper we propose a media access protocol for a WDM lightwave LAN/MAN assuming dual bus topology, which is an extension of the distributed queue dual bus (DQDB) network to the multichannel case. A large number of stations connected to both buses are supported using a small number of wavelengths. An approximate mathematical model is constructed to analyze the performance of the proposed protocol, with its accuracy verified by simulations. Numerical results show, that the protocol can achieve very good throughput and delay performance. Moreover, the network demonstrates better fairness in access delays than single-channel DQDB network.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Geometric view on colloidal interactions above the nematic-isotropic phase transition (2002)

Particles dispersed in a liquid crystal above the nematic-isotropic phase transition are surrounded by a surface-induced nematic wetting layer. When the nematic coronas of two particles overlap, they experience a strong attraction since the volume of nematic ordering and therefore the free energy is reduced. For normal anchoring of the liquid-crystal molecules on the particles' surfaces, we demonstrate that the implementation of this geometric view reproduces the Yukawa interaction derived by Galatola and Fournier in a recent paper [Phys. Rev. Lett. 86, 3915 (2001)], however with half the strength. To understand the factor 2, we rederive the Yukawa potential with the approximation of linear superposition of two one-particle profiles. At the end, we comment on the similarities of our approach to the screened electrostatic interaction of charged colloids.
Digitally Linearizable Compandors with Comments on "Project for a Digital Telephone Network" (1970)

A general model for digitally linearizable companding laws for use in digital communication systems is presented. The model is utilized to compare a ternary code presented by Neu and Kündig with a binary code. The primary considerations are ratio of signal to quantization noise, companding improvement, and linearization.
Filtration of Particulates in the Human Nose (1998)

This report reviews the current theories on the deposition of inhaled particulates in the human nose. The inhalation of particulates represents a challenge to the upper respiratory tract. The concentration of airborne particulates and pollutants varies between occupational and nonoccupational exposure. Regional deposition of potentially hazardous inhaled material determines the local and systemic toxicity. In vitro experiments using casting and molding techniques with water dye models or laser Doppler anemometry indicate that nasal airflow is determined by the size of the nasal valve and the turbinates. The main airstream during inspiration passes through the lower nasal passage. Filtration capacity of the human nose can be expressed mathematically or experimentally. In vivo experiments applying monodisperse or polydisperse aerosols during single or multiple respiratory cycles have shown by using microscopic or laser-optic identification that particles larger than 3 microm have a maximum deposition in the anterior part of the nose (nasal valve). Particles smaller than 3 microm and larger than 0.5 microm are filtered by the nasal mucosa and transported by cilia propulsion to the nasopharynx. The filtration for particles smaller than 0.5 microm is low. They seem to pass easily into the lower respiratory tract. This knowledge has an impact on nasal septal surgery and rhinoplasty. By influencing the structure of the nasal valve and the lower turbinates, the filtration capacity of the nose can be significantly decreased.
A cylindrically symmetric magnetic shield for a large‐bore 3.0 Tesla magnet (1993)

A 3.0 Tesla, 0.80-m bore magnet replaced our previous 1.9 Tesla, 0.76-m magnet. The 3.0 Tesla replacement magnet had a dipole moment of 1.7 with respect to that of the 1.9 Tesla magnet. The pre-existing cylindrically symmetric passive steel shielding was modified to confine the fringe fields of the replacement magnet to match those of the previous magnet. A cylindrically symmetric inner shield insert of about 20,000 kg was designed, fabricated, and installed. Upon energization of the magnet, the combined shielding met all design criteria. Alternative designs, optimization of cylindrically symmetric designs, and costs of fabrication, are presented herein.
Two-atom van der Waals interaction between polarizable/magnetizable atoms near magnetoelectric bodies (2007)

The van der Waals potential of two atoms in the presence of an arbitrary arrangement of dispersing and absorbing magnetoelectric bodies is studied. Starting from a polarizable atom placed within a given geometry, its interaction with a second polarizable/magnetizable atom is deduced from its Casimir-Polder interaction with a weakly polarizable/magnetizable test body. The general expressions for the van der Waals potential obtained thusly are illustrated by considering first the case of two atoms in free space, with special emphasis on the interaction between (i) two polarizable atoms and (ii) a polarizable and a magnetizable atom. Furthermore, the influence of magnetoelectric bodies on the van der Waals interaction is studied in detail for the example of two atoms placed near a perfectly reflecting plate or a magnetoelectric half space, respectively.
Stabilization of Interferon Messenger RNA Activity by Treatment of Cells with Metabolic Inhibitors and Lowering of the Incubation Temperature (1973)

Interferon production was induced in a strain of human diploid foreskin cells with poly(I).poly(C). Cycloheximide was included in the culture medium at the time of addition of the inducer. Actinomycin D was added to the cultures 4 or 5 hr later, before the inhibition of protein synthesis was reversed at 6 hr. Thus, subsequent interferon synthesis had to be directed by messenger RNA synthesized before addition of actinomycin D. The amount of interferon produced after such treatment was about 50-fold greater than in cells induced with poly(I).poly(C) but not treated with inhibitors. Experiments using cordycepin suggested that in spite of the continued presence of the inducer the synthesis of the bulk of interferon messenger RNA was completed within the first 2 hr of exposure of cells to poly(I).poly(C) and cycloheximide. Transcription of interferon messenger RNA was apparently not affected when interferon synthesis was suppressed to various degrees by different inhibitors of protein synthesis, indicating the independence of transcription and translation. The high rate of interferon synthesis after the reversal of cycloheximide action was more sustained at 32 degrees than at 37 degrees . The rate of decrease of overall protein synthesis in cells treated with actinomycin D and then incubated either at 32 degrees or 37 degrees showed a similar dependence on incubation temperature, suggesting that the stability of messenger RNA (or of another actinomycin D-sensitive component required for protein synthesis) was greater at the lower temperature.
A selective inhibitor of ceramide synthase 1 reveals a novel role in fat metabolism (2018)

Specific forms of the lipid ceramide, synthesized by the ceramide synthase enzyme family, are believed to regulate metabolic physiology. Genetic mouse models have established C16 ceramide as a driver of insulin resistance in liver and adipose tissue. C18 ceramide, synthesized by ceramide synthase 1 (CerS1), is abundant in skeletal muscle and suggested to promote insulin resistance in humans. We herein describe the first isoform-specific ceramide synthase inhibitor, P053, which inhibits CerS1 with nanomolar potency. Lipidomic profiling shows that P053 is highly selective for CerS1. Daily P053 administration to mice fed a high-fat diet (HFD) increases fatty acid oxidation in skeletal muscle and impedes increases in muscle triglycerides and adiposity, but does not protect against HFD-induced insulin resistance. Our inhibitor therefore allowed us to define a role for CerS1 as an endogenous inhibitor of mitochondrial fatty acid oxidation in muscle and regulator of whole-body adiposity.
An Edible Film of Lipids and Cellulose Ethers: Barrier Properties to Moisture Vapor Transmission and Structural Evaluation (1989)

ABSTRACT An edible, composite film of lipid and cellulose ethers was developed and appraised as a barrier to moisture vapor transmission. The film was comprised of a matrix of methylcellulose, hydroxypropyl meth‐ylcellulose and saturated C 16 and C 18 fatty acids, with a thin layer of white beeswax laminated to the surface. The edible film effectively retarded transport of moisture at water activities (a w ) up to at least 0.97 and maintained good barrier properties even when the a w on the low‐humidity side of the film was relatively high. The apparent activation energy for water vapor transmission through the edible film was 14.2 ± 2.5 kcal/mole. Electron microscopy revealed the importance of lipid morphology in determining moisture resistance lipid‐base films.
Immediate Implant Placement: Positives and Negatives (2010)

The immediate implant placement approach has been studied extensively since being introduced. Evidence available indicates that it is a successful procedure that may benefit patients. However, careful planning and case selection are needed to ensure implant success and final esthetic outcomes.
The MALE STERILITY1 Gene of Arabidopsis, Encoding a Nuclear Protein with a PHD-finger Motif, is Expressed in Tapetal Cells and is Required for Pollen Maturation (2002)

We identified the Arabidopsis MALE STERILITY1 (MS1) gene by transposon-mediated mutagenesis. In the transposon-inserted allele ms1-8, normal immature microspores separated from tetrads, but their subsequent maturation was abnormal: the outer layer of the microspore was absent, and both the microspore and the tapetal layer gradually became vacuolated. Empty locules resulted. The MS1 gene was expressed only in the tapetal layer during a very short period when the microspores were packed as tetrads. By the time the microspores had separated, the gene was no longer expressed. MS1 was not expressed in microspores. MS1 encodes a protein with a PHD-finger motif characteristic of some transcriptional regulators. A fusion protein consisting of the N-terminus of MS1 and green fluorescent protein was localized in the nucleus. These results suggest that MS1 protein is a nuclear signal molecule indispensable for pollen maturation.
Voice Encoding for the Space Shuttle Using Adaptive Delta Modulation (1978)

Two algorithms for voice encoding are described. One, the modified Abate, is a simplified version that is the type designed for the Space Shuttle. Waveforms of the response of the delta modulators to channel errors are given and performance data that shows the relationship between channel errors and word intelligibility are included. An analytic derivation yielding a comparison between PCM and adaptive delta modulation with respect to channel errors is also given.
Linkage of DNA Methylation Quantitative Trait Loci to Human Cancer Risk (2014)

Epigenetic regulation and, in particular, DNA methylation have been linked to the underlying genetic sequence. DNA methylation quantitative trait loci (meQTL) have been identified through significant associations between the genetic and epigenetic codes in physiological and pathological contexts. We propose that interrogating the interplay between polymorphic alleles and DNA methylation is a powerful method for improving our interpretation of risk alleles identified in genome-wide association studies that otherwise lack mechanistic explanation. We integrated patient cancer risk genotype data and genome-scale DNA methylation profiles of 3,649 primary human tumors, representing 13 solid cancer types. We provide a comprehensive meQTL catalog containing DNA methylation associations for 21% of interrogated cancer risk polymorphisms. Differentially methylated loci harbor previously reported and as-yet-unidentified cancer genes. We suggest that such regulation at the DNA level can provide a considerable amount of new information about the biology of cancer-risk alleles.
The Control of Humidity by Saturated Salt Solutions (1948)

A table of data gives percentage relative humidities over saturated solutions of eighty-eight different salts or salt mixtures. Remarks on the suitability of these solutions for humidity control are included.
Plasma level of atrial natriuretic peptide as an indicator of increased cardiac load in uremic patients. (1990)

Plasma levels of atrial natriuretic peptide (ANP) were measured by radioimmunoassay in 43 non-dialyzed uremic patients at rest and during maximal exercise to assess the possible relationship between plasma ANP levels and cardiac function, as judged by M-mode echocardiography and exercise tolerance. Patients with poor exercise capacity (exercise time less than 6 min) on dynamic exercise test had decreased left ventricular ejection fraction, increased left atrial diameter, and increased left ventricular mass index (LVMI), compared with patients with better exercise capacity (exercise time greater than 6 min). Plasma ANP was significantly higher in patients with poor exercise capacity and impaired cardiac function (202 pg/ml [95% confidence interval 119 to 284] at rest and 227 pg/ml [149 to 304] during exercise), compared with patients with better exercise capacity (75 pg/ml [50 to 102], p less than 0.005, and 123 pg/ml [80 to 167], p less than 0.05, respectively). Plasma ANP increased significantly (p less than 0.005) during exercise only in patients with better cardiac function. The best correlation among the variables studied was found between LVMI and plasma ANP concentration at rest (r = 0.56, p less than 0.001) and during exercise (r = 0.51, p less than 0.005), whereas neither blood pressure nor renal function showed any significant correlation with ANP levels. We conclude that plasma ANP levels are elevated in uremic patients with impaired cardiac function, correlating with increased LVMI. Plasma ANP determinations are useful in identifying increased cardiac load and consequent cardiac hypertrophy and dysfunction with known associations with increased cardiovascular mortality in patients with chronic renal failure.