gebhart/patrepeval huggingface dataset:
Rat-2 fibroblasts express specific adrenomedullin receptors, but not calcitonin-gene-related-peptide receptors, which mediate increased intracellular cAMP and inhibit mitogen-activated protein kinase activity (1999)

Rat-2 fibroblasts demonstrate specific binding of 125I-labelled rat adrenomedullin (KD=0.43 nM; Bmax=50 fmol/mg of protein) in the absence of 125I-labelled calcitonin-gene-related peptide (CGRP) binding. Therefore Rat-2 cells were used to examine the pharmacology and signal transduction pathways of adrenomedullin receptors. We examined the effects of adrenomedullin, the CGRP receptor antagonist CGRP-(8-37) and the amylin antagonists AC187 and AC253 on receptor binding and cAMP production. AC253, AC187 and CGRP-(8-37) inhibited 125I-adrenomedullin binding, with respective IC50 values of 25+/-8, 129+/-39 and 214+/-56 nM. Adrenomedullin dose-dependently increased intracellular cAMP (approximate EC50=1.0 nM). CGRP-(8-37), AC253 and AC187 antagonized adrenomedullin-stimulated cAMP production at micromolar concentrations. Using kinase-substrate assays, Mono Q FPLC and 'phospho-specific' Western blotting, we found that adrenomedullin alone abolished basal mitogen-activated protein kinase (MAPK) activity and dose-dependently inhibited platelet-derived-growth-factor-stimulated MAPK activity. Radioimmunoassay for adrenomedullin of media from Rat-2 cells showed a linear release of adrenomedullin-like immunoreactivity of 3.1 fmol/h per 2x10(6) cells. Gel-filtration chromatography showed that this adrenomedullin-like immunoreactivity co-eluted with synthetic rat adrenomedullin. Northern blotting with a rat adrenomedullin cDNA probe was used to confirm the presence of adrenomedullin mRNA. However, neither Northern blotting nor reverse transcriptase-PCR showed the presence of the cloned adrenomedullin receptor (L1). We conclude that the Rat-2 cell line expresses a specific adrenomedullin receptor (coupled to cAMP production and regulation of MAPK) and secretes adrenomedullin, which may participate in a regulatory control loop.
A new approach to micromagnetic simulation of thermal magnetic fluctuation noise in magnetoresistive read sensors (2005)

Thermal magnetic fluctuation noise forms the ultimate application limit of small magnetoresistive devices for magnetic data storage. The noise analysis of these devices becomes increasingly important for high-density recording. Such noise analyses by micromagnetic simulation are, however, computationally very intensive and require enormous amounts of simulation time. This paper presents a faster micromagnetic method to arrive at the noise and small signal dynamics of these sensors. It uses, for every cell in the simulation, a behavioral analog (i.e., an "analog computer" model) described by the same equations as the basic magnetic simulation cell (the Landau-Lifshitz-Gilbert equation, Slonczewski's spin torque addition and the equipartition principle). The sensor, as a network of exchange and demagnetization coupled cells, is then solved with a network simulator (such as PSpice). This process gives a drastic reduction in computing time and yet leads to high resolution spectra with very little residual uncertainty. The paper further presents a large number of simulation results for uniform sensors as well as for sensors with a nonuniform magnetic bias and a nonuniform electrical bias. It addresses the spatial distribution of the noise (standing spin waves in the noise) and the correlation of the noise in various parts of the sensors. Finally, as a further example of this method, the paper shows the effect of spin torque transfer on the noise and the small signal dynamics of a current perpendicular to plane giant magnetoresistive (CPP GMR) sensor.
Translocation t(2;7)(p12;q21-22) with dysregulation of the CDK6 gene mapping to 7q21-22 in a non-Hodgkin's lymphoma with leukemia. (2002)

We have demonstrated that CDK6 expression is dysregulated even when the breakpoint on 7q21-22 is located 66kb upstream from the coding region. Interestingly, the precise assignment of the lymphoma type in our case was not possible even when the splenic histology was analyzed.
Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing (2017)

Recent advances in single-cell, transcriptomic profiling have provided unprecedented access to investigate cell heterogeneity during tissue and organ development. In this study, we used massively parallel, single-cell RNA sequencing to define cell heterogeneity within the zebrafish kidney marrow, constructing a comprehensive molecular atlas of definitive hematopoiesis and functionally distinct renal cells found in adult zebrafish. Because our method analyzed blood and kidney cells in an unbiased manner, our approach was useful in characterizing immune-cell deficiencies within DNA-protein kinase catalytic subunit (<i>prkdc</i>), interleukin-2 receptor γ a (<i>il2rga</i>), and double-homozygous-mutant fish, identifying blood cell losses in T, B, and natural killer cells within specific genetic mutants. Our analysis also uncovered novel cell types, including two classes of natural killer immune cells, classically defined and erythroid-primed hematopoietic stem and progenitor cells, mucin-secreting kidney cells, and kidney stem/progenitor cells. In total, our work provides the first, comprehensive, single-cell, transcriptomic analysis of kidney and marrow cells in the adult zebrafish.
Inhibition of azoxymethane‐induced experimental colon carcinogenesis in wistar rats by 6‐hydroxydopamine (1992)

The effects of 6-hydroxydopamine (6-OHDA) on the incidence, number and histology of colon tumors induced by azoxymethane (AOM), and on norepinephrine (NE) concentration in the colon wall and the labelling index of the colon mucosa were investigated in Wistar rats. Rats received sub-cutaneous (s.c.) injections of AOM once a week for 10 weeks, and throughout 35 weeks were also given intraperitoneal (i.p.) injections of 6-OHDA. Prolonged administration of 6-OHDA was found to cause a significant reduction in the incidence and number of colon tumors. However, it had no influence on the histological features or depths of involvement of colon tumors and/or adenocarcinomas. Its administration also caused significant decreases in the NE concentration in the colon wall and in the labelling index of the colon mucosa. Our findings indicate that 6-OHDA has a protective effect against colon carcinogenesis, and that the activity of the sympathetic nervous system may have an important influence on colon carcinogenesis.
Short-Term Exposure to Membrane-Active Antibiotics Inhibits <i>Cryptosporidium parvum</i> Infection in Cell Culture (2000)

A cell culture system and double fluorogenic staining were used to study the susceptibility of Cryptosporidium parvum to membrane-active antibiotics. Buforin II and magainin II exerted a cytotoxic effect on sporozoites but did not consistently affect oocyst viability. Lasalocid and nigericin demonstrated less activity against sporozoites but reduced the infectivity of oocysts.
Six Phase Synchronous Machine with AC and DC Stator Connections, Part I: Equivalent Circuit Representation and Steady-State Analysis (1983)

This paper describes a detailed circuit representation of a six phase synchronous machine wherein mutual leakage couplings between the two sets of three phase stator windings are included. The main modes of power transfer of a six phase machine with sinusoidal inputs are examined. A harmonic phasor method of steady-state analysis to obtain the current, voltage and torque waveforms of a six phase machine with mixed AC-DC stator connections is described. It is shown that the output waveforms for various operating conditions from a digital computer program using the harmonic phasor method agree remarkably well with those obtained from an established detailed analog simulation.
Maximum likelihood genetic sequence reconstruction from oligo content (1994)

Abstract One promising technique for determining long genetic sequences is sequencing by oligonucleotide content. This technique involves probing a segment of the unknown multimillion “character” genetic sequence for the presence or absence of known short subsequences. The information obtained from such hybridization experiments may be represented in network form. Network optimization methods may then be applied to identify the most likely forms of the unknown target sequence. © 1994 by John Wiley & Sons, Inc.
Increased Akt and phosphorylated Akt expression are associated with malignant biological features of prostate cancer in Japanese men (2007)

Increased expression of both Akt and p-Akt were associated with higher tumour grade as well as a higher AR-staining score and Ki67 LI. These data indicate that Akt and p-Akt might be molecular markers for detecting malignant biological features of prostate cancer in the Japanese population.
Fluoroquinolone Action against Clinical Isolates of <i>Mycobacterium tuberculosis</i> : Effects of a C-8 Methoxyl Group on Survival in Liquid Media and in Human Macrophages (1999)

When the lethal action of a C-8 methoxyl fluoroquinolone against clinical isolates of Mycobacterium tuberculosis in liquid medium was measured, the compound was found to be three to four times more effective (as determined by measuring the 90% lethal dose) than a C-8-H control fluoroquinolone or ciprofloxacin against cells having a wild-type gyrA (gyrase) gene. Against ciprofloxacin-resistant strains, the C-8 methoxyl group enhanced lethality when alanine was replaced by valine at position 90 of the GyrA protein or when aspartic acid 94 was replaced by glycine, histidine, or tyrosine. During infection of a human macrophage model by wild-type Mycobacterium bovis BCG, the C-8 methoxyl group lowered survival 20- to 100-fold compared with the same concentration of a C-8-H fluoroquinolone. The C-8 methoxyl fluoroquinolone was also more effective than ciprofloxacin against a gyrA Asn94 mutant of M. bovis BCG. In an M. tuberculosis-macrophage system the C-8 methoxyl group improved fluoroquinolone action against both quinolone-susceptible and quinolone-resistant clinical isolates. Thus, a C-8 methoxyl group enhances the bactericidal activity of quinolones with N1-cyclopropyl substitutions; these data encourage further refinement of fluoroquinolones as antituberculosis agents.
18세기 프랑스 면직물 고찰 -직물견본수집록 「리슐리유 컬렉션」을 중심으로- (2010)

본 연구는 면직물의 다양한 유형을 수록하고 있는「리슐리유 컬렉션」을 통해, 18세기 초중반 프랑스 면직물에 관한 연구를 목적으로 하며, 연구 방법은 다음과 같다. 첫째, 선행연구를 토대로 18세기 프랑스 직물의 유형 및 면 프린트 직물 산업과 기술에 대한 역사적 배경을 고찰하였다. 둘째,「리슐리유 컬렉션」의 직물 견본 총 4818개 중 분류된 면직물 264점의 목록을 작성, 유형 및 명칭에 따른 역사, 조성 및 조직 특성, 용도, 생산지 등을 조사하고, 18세기 직물에 관한 문헌 및 선행연구와 종합하여 그 제반 특성을 연구하였다. 뚜왈, 쉬노아즈, 시아모아즈, 엥디엔느 등 다양한 기법의 프린트 직물이 215점으로 수집된 견본의 주를 이루고 있다. 조성에 따른 직물의 종류는 뚜왈, 꾸띠, 퓌뗀느, 다마는 교직하지 않고 면으로만 제직되었고, 꺄디는 모, 바젱은 마, 뽀쁠린과 빠스멍띠에는 견과 각각 교직하였다. 조직에 따른 직물의 종류로는 뚜왈, 부딴느, 꾸띠, 뽀쁠린은 평직, 퓌뗀느, 바젱, 꺄디는 능직, 다마는 변형 능직으로 이루어졌으며, 빠스멍띠에는 특수 조직으로 이루어진 장식끈이다. 뚜왈, 쉬노아즈, 시아모아즈, 엥디엔느 등의 프린트 직물을 포함한 대부분의 직물들은 옷감으로 사용되었다. 섬세하게 짠 퓌뗀느는 안감, 모와 교직한 기모직의 꺄디는 겨울옷감, 견과 교직하여 섬세하게 제직한 뽀쁠린은 란제리류로 사용되었다. 마와 섞어 굵게 짠 꾸띠는 콜셋에 사용되었고, 부딴느, 바젱, 꾸띠, 다마는 침장구용으로 쓰였다. 수록된 직물의 주 생산지는 프랑스 북부의 루앙, 메슬레, 아베빌, 젠느와 중부의 오를레앙 그리고 남부의 마르세이유, 앙띠브, 프로방스, 뻬르삐냥, 뚤롱 등이다.
Overcoming the data-scale management challenge in large, dynamic networks (2005)

A large military network may deliver hundreds of applications to thousands of users across many tens of thousands of devices, under conditions of constant and unpredictable change. This poses insuperable problems for traditional network management systems, which attempt to assemble a coherent view of infrastructure operations and their impact on application performance and business processes by aggregating data from thousands of infrastructure elements, agents, and probes into a monolithic master database. Such an approach cannot address the fundamental data-scale management challenge posed by large, dynamic networks: making sense of the sheer volume of data that must be collected, aggregated, and stored to manage such networks; and there are significant additional obstacles to success. These problems can be overcome with a distributed, flow-based approach using techniques adapted from high-energy physics research to address the data-scale management challenge.
Dietary fat and immune function. I. Antibody responses, lymphocyte and accessory cell function in (NZB x NZW)F1 mice. (1985)

The influence of dietary fat on autoimmunity in lupus-prone (NZB x NZW)F1 mice has been demonstrated. In defining further the effects of dietary lipid on the immune system of this strain, female weanling mice were placed on four diets differing in quantity and type of fat. Their immunologic response was then studied by a variety of tests at 4 and 7 mo of age. Few differences were seen among the four groups at 4 mo of age. At 7 mo of age, however, the mice receiving diets high in saturated and unsaturated fats had a reduced mitogenic response to T cell mitogens and an enhanced response to the B cell mitogen LPS. Immunoglobulin levels and delayed hypersensitivity responses did not show any consistent differences among the diet groups. At 7 mo, however, mice receiving diets high in unsaturated fat demonstrated hyperresponsiveness to injected sheep red blood cells as measured by the hemolytic plaque technique. In addition, peritoneal leukocytes from the same diet group exhibited an increased response to bromelain-treated autologous erythrocytes which was decreased after treatment with anti-Thy-1 antiserum and complement. Phagocytosis by peritoneal macrophages was significantly decreased in the animals fed high-fat diets, particular high saturated fat. Similarly, natural killer cell activity was markedly reduced in the mice with a high intake of saturated lipid, a finding which correlated with the in vitro production of interferon. These results indicate that diets high in fat influence immune responses and thus can affect the onset and severity of autoimmune disease. A low-fat diet can reduce the development of disease by maintaining normal immune responses. The data also suggest that unsaturated fat may influence T helper cell activity and therefore antibody production, whereas saturated fats may affect cellular immune responses which are dependent on membrane contact.
Sustained and NK/CD4+ T Cell-Dependent Efficient Prevention of Lung Metastasis Induced by Dendritic Cells Harboring Recombinant Sendai Virus (2009)

We recently demonstrated efficient antitumor immunity against murine tumors using dendritic cells (DCs) activated by recombinant Sendai viruses (rSeVs), and proposed a new concept, "immunostimulatory virotherapy," for cancer immunotherapy. However, there has been little information on the efficacy of this method in preventing metastatic diseases. In this study, we investigated the efficacy of vaccinating DCs activated by fusion gene-deleted nontransmissible rSeV (rSeV/dF) using a murine model of lung metastasis. Bolus and i.v. administration of DCs harboring rSeV/dF-expressing GFP without pulsation of tumor Ag (DC-rSeV/dF-GFP) 2 days before tumor inoculation showed efficient prevention against lung metastasis of c1300 neuroblastoma, but not of RM-9 prostatic cancer. We found that the timing of DC therapy was critical for the inhibition of pulmonary metastasis of RM-9, and that the optimal effect of DCs was seen 28 days before tumor inoculation. Interestingly, the antimetastatic effect was sustained for over 3 mo, even when administered DCs were already cleared from the lung and organs related to the immune system. Although NK cell activity had already declined to baseline at the time of tumor inoculation, Ab-mediated depletion studies revealed that CD4+ cells as well as the presence of, but not the activation of, NK cells were crucial to the prevention of lung metastasis. These results are the first demonstration of efficient inhibition of lung metastasis via bolus administration of virally activated DCs that was sustained and NK/CD4+ cell-dependent, and may suggest a potentially new mechanism of DC-based immunotherapy for advanced malignancies.
Selective, reversible, reagentless maltose biosensing with core–shell semiconducting nanoparticles (2005)

Reagentless and reversible maltose biosensors are demonstrated using ZnS coated CdSe (CdSe@ZnS) nanoparticle emission intensities. This method is based on electron transfer quenching of unimolecular protein-CdSe@ZnS nanoparticle assemblies, which is provided by a protein-attached Ru(II) complex. This Ru(II) complex is presumed to reduce a valence band hole of the CdSe@ZnS excited state by tunneling through the ZnS overcoating. The Ru(II) complex mediated quenching of CdSe@ZnS nanoparticle emission was only decreased 1.2-fold relative to the CdSe nanoparticle systems. While four different Ru(II) complex attachment sites provided different amounts of nanoparticle emission quenching (1.20 to 1.75-fold decrease), all of these attachment sites yielded maltose-dependent intensity changes (1.1 to 1.4-fold increase upon maltose addition). Maltose dissociation constants for these four biosensing systems range from 250 nM to 1.0 microM, which are similar to the maltose-maltose binding protein dissociation constant that these sensors are based on. The increased fluorescence intensity was found to only occur in the presence of maltose. Furthermore, the ability of these reagentless protein-nanoparticle assemblies to perform maltose biosensing reversibly is demonstrated with the addition of alpha-glucosidase. Three 50 microM maltose additions after alpha-glucosidase addition showed increases of 2.2 microM, 600 nM, and 150 nM maltose. This result demonstrates a fluorometric method for examining alpha-glucosidase activity. Using maltose binding protein to control Ru(II) complex interactions with CdSe@ZnS nanoparticle surfaces provide a novel class of highly fluorescent, photostable biosensors that are selective for maltose.
Dynamic crosstalk cancellation for binaural synthesis in virtual reality environments (2006)

A system is described that combines virtual imaging by binaural synthesis and reproduction over loudspeakers using a dynamic head-tracked crosstalk cancellation (CTC) for the listener who is interacting with a common visual virtual reality (VR) application in real time. To provide a full 360° rotation for the listener, the two-loudspeaker dynamic CTC solution was expanded to four channels. This acoustical system is designed for integration into a visual VR system that produces holographic video images and enables the generation of congruent visual and acoustical scenes.
Frequency and profile of objective cognitive deficits in hospitalized patients recovering from COVID-19 (2020)

Abstract Background Cognitive impairment is common following critical illness. A number of case reports and case series have suggested that cognitive deficits occur in patients with COVID-19. This study evaluated the frequency, severity, and profile of cognitive dysfunction in hospitalized patients recovering from COVID-19. Methods We obtained and analyzed cross-sectional neuropsychological data from a cohort of N=57 patients participating in inpatient rehabilitation. Our primary outcome measure was the Brief Memory and Executive Test (BMET). We calculated the frequency of impairment based on clinician diagnosis and by the BMET subtests using age-normed classification of impairment. We explored associations with intubation and extubation as markers of illness severity and complications, as well as psychiatric diagnosis. Outcomes Our sample was 75% male, 61% non-white, with a mean age of 64.5 (SD = 13.9) years. Patients were evaluated at a mean of 43.2 days post-admission. 88% had documented hypoxemic respiratory failure and 77% required intubation. 81% of patients had cognitive impairment, ranging from mild to severe. Deficits were most common in working memory (55% of patients impaired), set-shifting (47%), divided attention (46%), and processing speed (40%). Executive dysfunction was not significantly associated with intubation length or the time from extubation to assessment, nor was it associated with the presence of a psychiatric diagnosis. Interpretation Medically stable inpatients recovering from COVID-19 commonly have deficits in attention and executive functions. These deficits were not significantly correlated with length of intubation or time since extubation. Findings provide an early benchmark for studying the evolution of cognitive difficulties after COVID-19 and suggest that easy to disseminate interventions that remediate attention and executive dysfunctions may be important in this population. Funding The authors have no funding for this study to report.
"Chip-size" antennas for implantable sensors and smart dust (2005)

In this paper, we present the analysis, design and experimental results of /spl lambda//4-patch antennas operating at /spl sim/10 GHz. The ground plane has been adjusted to the patch area in order to drastically reduce the antenna size. Several geometries are presented, with HFSS simulation results. Impedance matching calculations for maximum power transfer between the antenna and the rectifying circuit is also presented. For a 4/spl times/5/spl times/1.6 mm/sup 3/ rectangular antenna fabricated on an RT/Duroid 5880 PCB, the measured maximum gain is 0.75 dB/sub i/ at 10.3 GHz.
ChemInform Abstract: A New Fungal Metabolite, Sch 202596, with Inhibitory Activity in the Galanin Receptor GALR1 Assay. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A robustness and audibility analysis of a temporal envelope modulating audio watermark (2005)

Audio watermarking techniques can be used to embed extra information into audio signals. The goal is to hide pre-specified data carrying some information into the audio stream such that it is not audible to the human ear (i.e., transparent) and is, at the same time, resistant to signal processing and removal attacks (i.e., robust). In the currently known watermarking systems, the above challenge is not always adequately resolved. This and other practical considerations such as complexity have motivated us to look into other alternatives to audio watermarking. In this paper, we present a new watermarking system referred to as MASK (modified audio signal keying) that has resulted from this study. In MASK, the short-term envelope of the audio signal is modified in such a way that the change is imperceptible to the human listener. Results of informal experiments do not only confirm this, but also show that MASK has a good robustness behavior.