gebhart/patrepeval huggingface dataset:
Role of L-arginine-nitric oxide pathway in myocardial reoxygenation injury (1992)

In view of the recent findings that NO reacts with superoxide anion to generate hydroxyl radical, the present study was conducted to ascertain the role of endogenous NO in mediating myocardial reoxygenation injury in the hypoxic piglet on cardiopulmonary bypass. Anesthetized piglets were made hypoxic (PaO2 = 20-30 mmHg) for up to 120 min, followed by reoxygenation on cardiopulmonary bypass for 30 min. Reoxygenation caused rapidly developing myocardial injury characterized by decreased contractility (expressed as end-systolic elastance) and increased lipid peroxidation (measured as conjugated dienes). Systemic venous and coronary sinus blood content of NO decreased significantly during hypoxia and increased substantially above prehypoxic levels during reoxygenation on cardiopulmonary bypass. Administration of either the antioxidants mercaptopropionyl glycine and catalase or the NO synthase inhibitor, NG-nitro-L-arginine methyl ester, to the extracorporeal circuit afforded similar and nearly complete protection against myocardial reoxygenation injury. The protective effects of NG-nitro-L-arginine methyl ester were nullified by adding an excess of L-arginine to the pump circuit, suggesting that the L-arginine-NO pathway is involved in myocardial reoxygenation injury.
Highly Diastereoselective α-Mannopyranosylation in the Absence of Participating Protecting Groups (2000)

S-Phenyl 2,6-di-O-benzyl-3,4-O-(2',3'-dimethoxybutane-2', 3'-diyl)-1-thia-alpha-D-mannopyranoside and its sulfoxide, following activation at -78 degrees C with benzenesulfenyl triflate or triflic anhydride, respectively, provide the corresponding alpha-mannosyl triflate as demonstrated by NMR spectroscopy. On addition of an acceptor alcohol alpha-mannosides are then formed. Similarly, S-phenyl 2,3-O-carbonyl-4, 6-O-benzylidene-1-thia-alpha-D-mannopyranoside and ethyl 3-O-benzoyl-4, 6-O-benzylidene-2-O-(tert-butyldimethylsilyl)-1-thia-alpha-D-mannopyr anoside both provide alpha-mannosides on activation with benzenesulfenyl triflate followed by addition of an alcohol. These results stand in direct contrast to the highly beta-selective couplings of comparable glycosylations with 2,3-di-O-benzyl-4, 6-O-benzylidene protected mannosyl donors and draw attention to the subtle interplay of reactivity and structure in carbohydrate chemistry.
Marketing Myopia: An Integrated View (1993)

Outlines the foundations and dimensions of marketing myopia and proposes a two‐dimensional classification of its several types. Presents an integrated analysis which is intended to enrich, rather than replace, the existing explanations and to clarify and reinforce a caveat proposed 30 years ago. Makes a recommendation for seeking innovative marketing strategies which are designed to remove some of the myopia‐caused restrictions on the range of strategic options.
60-GHz-band LTCC module technology for wireless gigabit transceiver applications (2005)

This paper presents 60-GHz-band module technology for gigabit wireless systems. All millimeter-wave components described here are flip-chip mountable devices, providing highly repeatable interconnects even for such a high-frequency range. For multi-chip modules, multi-layer LTCC substrates with cavity structures are employed, where MMICs, filters and dielectric resonator oscillators are mounted. Once the module fabrication is completed, only DC feeding and baseband I/O should be cared to connect with printed wiring boards. For 60-GHz-band ASK modules, modulation/demodulation with a speed more than 1 Gb/s and an output power of 10 mW are achieved. The modules are implemented in several applications. The uncompressed high-definition video transmission systems are highlighted.
Predictive tools in VLSI system design: timing aspects (2003)

A major problem in hierarchical design is to achieve consistency of the design steps that will not require iterations and will converge to the 'reasonably good' solution. To achieve this goal, additional efforts need to be made of each level of the hierarchical top-down process to derive constraints on variables of the lower level of hierarchy and use these additional constraints to solve the problems of lower levels. The authors illustrate this concept with the design step positioned between the logical level of simulation for VLSI and the physical implementation of the design. This step performs the timing analysis of the logic and provides constraints for the physical implementation of the design. If these constraints are satisfied on the layout phase, then timing-error-free design is obtained.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Development of a Synbiotic Beverage Enriched with Bifidobacteria Strains and Fortified with Whey Proteins (2017)

The objective of this study was to develop a new synbiotic beverage evaluating the ability of some bifidobacteria strains to grow in this beverage which was fortified with whey proteins up to 20 g L<sup>-1</sup>, and enriched with 10 g L<sup>-1</sup> of prebiotic inulin or resistant starch. The ability of <i>Bifidobacterium</i> strains to survive for 30 days at 4°C was evaluated in two synbiotic whey protein fortified beverages formulated with 2% of whey proteins and 1% of inulin or resistant starch. Microbial growth was significantly affected by the whey protein amount as well as by the kind of prebiotic fiber. Resistant starch promoted the growth of the <i>Bifidobacterium pseudocatenulatum</i> strain and its viability under cold storage, also conferring higher sensory scores. The development of this new functional beverage will allow to carry out <i>in vivo</i> trials in order to validate its pre- and probiotic effects.
Influence of the multisine excitation amplitude design for biomedical applications using Impedance Spectroscopy (2011)

Electrical Impedance Spectroscopy (EIS) is a powerful tool to collect data from many biological materials in a wide variety of applications. Body composition fluid or tissue and organ state monitoring are just some examples of these applications. While the classical EIS is based on frequency sweep, the EIS technique using broadband excitations allows to acquire simultaneous impedance spectrum data. The strength and weakness of broadband EIS relies on the fact that it enables multiple Electrical Bio-Impedance (EBI) data collection in a short measuring time but at the cost of losing impedance spectrum accuracy. In general, there is a relationship between the broadband excitation time/frequency properties and the final EBI's accuracy obtained. This paper studies the influence of the multisine broadband excitation amplitude's design over the EBI accuracy by means of the resultant Noise-to-Signal Ratio (NSR) obtained when measuring with a custom impedance analyzer. Theory has been supported by a set of validation experiments.
Program Fragmentation as a Metamorphic Software Protection (2007)

Unauthorized reverse-engineering of programs and algorithms is a major problem for the software industry. Reverse-engineers search for security holes in the program to exploit or try to steal competitors' vital algorithms. To discourage reverse-engineering, developers use a variety of static software protections to obfuscate their programs. Metamorphic software protections add another layer of protection to traditional static obfuscation techniques, forcing reverse-engineers to adjust their attacks as the protection changes. Program fragmentation combines two obfuscation techniques, outlining and obfuscated jump tables, into a new, metamorphic protection. Sections of code are removed from the main program flow and placed throughout memory, reducing the program's locality. These fragments move and are called using obfuscated jump tables, making program execution difficult to follow. This research assesses the performance overhead of a program fragmentation engine and provides analysis of its effectiveness against reverse-engineering techniques. Results show that program fragmentation has low overhead and is an effective technique to complicate disassembly of programs using two common disassembler/debugger tools.
Renal failure after major angiography can be avoided with hydration (1981)

A reported 12% incidence of acute renal failure after angiography prompted this prospective study to substantiate or repudiate this seemingly excessive rate. In 537 consecutive patients undergoing cerebral, abdominal, or peripheral angiography, there was no instance of renal failure following the procedure. The results of this study indicate that when adequate hydration is maintained, angiography does not pose a "significant hazard" of renal failure as previously reported, even in patients with underlying medical problems.
Denaturation and Renaturation of Self-Assembled Yeast Iso-1-cytochrome <i>c</i> on Au (2004)

We have made surface plasmon resonance (SPR) measurements of yeast iso-1-cytochrome c (Cyt c) on a gold surface. Angle-resolved SPR curves are recorded as a function of urea concentration before and after self-assembly of the Cyt c. Exposure to a urea solution causes denaturation of Cyt c, which shifts the minimum in the SPR curve to a larger angle and decreases the signal amplitude. The Gibbs free energy change for denaturation of the protein on Au is calculated from the change of the SPR signal amplitude with urea concentration. We find that (1) Cyt c can be reversibly denatured and renatured, depending on the urea concentration, and (2) the Gibbs free energy change for denaturation of Cyt c on Au surface in water, DeltaG degrees (water), is 1.5 kcal/mol, which is approximately 4 times less than that in bulk solution.
Predicting Intent Using Activity Logs (2017)

People have different intents in using online platforms. They may be trying to accomplish specific, short-term goals, or less well-defined, longer-term goals. While understanding user intent is fundamental to the design and personalization of online platforms, little is known about how intent varies across individuals, or how it relates to their behavior. Here, we develop a framework for understanding intent in terms of goal specificity and temporal range. Our methodology combines survey-based methodology with an observational analysis of user activity. Applying this framework to Pinterest, we surveyed nearly 6000 users to quantify their intent, and then studied their subsequent behavior on the web site. We find that goal specificity is bimodal - users tend to be either strongly goal-specific or goal-nonspecific. Goal-specific users search more and consume less content in greater detail than goal-nonspecific users: they spend more time using Pinterest, but are less likely to return in the near future. Users with short-term goals are also more focused and more likely to refer to past saved content than users with long-term goals, but less likely to save content for the future. Further, intent can vary by demographic, and with the topic of interest. Last, we show that user's intent and activity are intimately related by building a model that can predict a user's intent for using Pinterest after observing their activity for only two minutes. Altogether, this work shows how intent can be predicted from user behavior.
A new method for the determination of the relative acidities of alcohols in alcoholic solutions. The nucleophilicities and competitive reactivities of alkoxides and phenoxides (1979)

A new semiquantitative method has been developed for measuring the relative acidities of methanol, ethanol, isopropyl alcohol, and tert-butyl alcohol in mixed hydroxylic solvents. A solution of the alkoxides of two alcohols in an excess of the two alcohols is allowed to react with n-butyl bromide to form a mixture of two ethers. The composition of the ether mixture is a measure of the "competitive reactivity" of thetwo alkoxides. This can be measured directly, and in theory can be factored into two components: the relative nucleophilicity and the relative basicity of the two alkoxides. Relative nucleophilicities are determined by using solutions in which phenol is one component. Knowing the competitive reactivities and nucleophilicities, the relative acidities of methanol, ethanol, isopropyl alcohol, and tert-butyl alcohol in alcoholic media are shown to be 4.4, 1.0, 0.24, and 0.21, respectively. The relative nucleophilicities of hydroxide, methoxide, ethoxide, isopropoxide, tert-butoxide, phenoxide, and m-cresoxide are 0.08, 0.82, 1.0, 0.4, 0.04, 0.46, and 0.57 respectively.
The mechanism of the drug induced partial displacement of methyl green from DNA. (1988)

The displacement of methyl green, a dye bound ionically to double stranded DNA, has been suggested as a potential assay for drug-DNA interaction. The present investigation studies the equilibrium system of methyl green, its colorless carbinol form and DNA in the presence of salt (MgSO4), ethidium bromide, and quinine. The reversibility of the equilibrium, and the absence of sequestered sites or two modes of binding for methyl green are demonstrated.
Capillary Electrophoresis Chips with a Sheath-Flow Supported Electrochemical Detection System (2004)

Microfabricated capillary electrophoresis chips containing an integrated sheath-flow electrochemical detector are developed with the goal of minimizing the influence of separation voltages on end-column detection while maintaining optimum performance. The microdevice consists of an upper glass wafer carrying the etched separation, injection, and sheath-flow channels and a lower glass wafer on which gold- and silver-plated electrodes have been fabricated. The sheath-flow channels join the end of the separation channel from each side, and gravity-driven flow carries the analytes to the electrochemical detector placed at working distances of 100, 150, 200, and 250 microm from the separation channel exit. The performance of this detector is evaluated using catechol and a detection limit of 4.1 microM obtained at a working distance of 250 microm. Detection of DNA restriction fragments and PCR product sizing is demonstrated using the electroactive intercalating dye, iron phenanthroline. Additionally, an allele-specific, PCR-based single-nucleotide polymorphism typing assay for the C282Y substitution diagnostic for hereditary hemochromatosis is developed and evaluated using ferrocene-labeled primers. This study advances the feasibility of high-speed, high-throughput chemical and genetic analysis using microchip electrochemical detection.
Three-Dimensional Crystalline and Homogeneous Metallic Nanostructures Using Directed Assembly of Nanoparticles (2014)

Directed assembly of nano building blocks offers a versatile route to the creation of complex nanostructures with unique properties. Bottom-up directed assembly of nanoparticles have been considered as one of the best approaches to fabricate such functional and novel nanostructures. However, there is a dearth of studies on making crystalline, solid, and homogeneous nanostructures. This requires a fundamental understanding of the forces driving the assembly of nanoparticles and precise control of these forces to enable the formation of desired nanostructures. Here, we demonstrate that colloidal nanoparticles can be assembled and simultaneously fused into 3-D solid nanostructures in a single step using externally applied electric field. By understanding the influence of various assembly parameters, we showed the fabrication of 3-D metallic materials with complex geometries such as nanopillars, nanoboxes, and nanorings with feature sizes as small as 25 nm in less than a minute. The fabricated gold nanopillars have a polycrystalline nature, have an electrical resistivity that is lower than or equivalent to electroplated gold, and support strong plasmonic resonances. We also demonstrate that the fabrication process is versatile, as fast as electroplating, and scalable to the millimeter scale. These results indicate that the presented approach will facilitate fabrication of novel 3-D nanomaterials (homogeneous or hybrid) in an aqueous solution at room temperature and pressure, while addressing many of the manufacturing challenges in semiconductor nanoelectronics and nanophotonics.
Total Synthesis of Verruculogen and Fumitremorgin A Enabled by Ligand-Controlled C–H Borylation (2015)

Verruculogen and fumitremorgin A are bioactive alkaloids that contain a unique eight-membered endoperoxide. Although related natural products such as fumitremorgins B and C have been previously synthesized, we report the first synthesis of the more complex, endoperoxide-containing members of this family. A concise route to verruculogen and fumitremorgin A relied not only on a hydroperoxide/indole hemiaminal cyclization, but also on the ability to access the seemingly simple starting material, 6-methoxytryptophan. An iridium-catalyzed C-H borylation/Chan-Lam procedure guided by an N-TIPS group enabled the conversion of a tryptophan derivative into a 6-methoxytryptophan derivative, proving to be a general way to functionalize the C6 position of an N,C3-disubstituted indole for the synthesis of indole-containing natural products and pharmaceuticals.
ChemInform Abstract: Cycloaddition Elimination Reactions of 4‐Aryl‐5‐arylimino‐1,2,4‐ dithiazolidin‐3‐ones (Mustard Oil Oxides). (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Requirement For C-terminal Sequences in Regulation of Ect2 Guanine Nucleotide Exchange Specificity and Transformation (2004)

Ect2 was identified originally as a transforming protein and a member of the Dbl family of Rho guanine nucleotide exchange factors (GEFs). Like all Dbl family proteins, Ect2 contains a tandem Dbl homology (DH) and pleckstrin homology (PH) domain structure. Previous studies demonstrated that N-terminal deletion of sequences upstream of the DH domain created a constitutively activated, transforming variant of Ect2 (designated DeltaN-Ect2 DH/PH/C), indicating that the N terminus served as a negative regulator of DH domain function in vivo. The role of sequences C-terminal to the DH domain has not been established. Therefore, we assessed the consequences of mutation of C-terminal sequences on Ect2-transforming activity. Surprisingly, in contrast to observations with other Dbl family proteins, we found that mutation of the invariant tryptophan residue in the PH domain did not impair DeltaN-Ect2 DH/PH/C transforming activity. Furthermore, although the sequences C-terminal to the PH domain lack any known functional domains or motifs, deletion of these sequences (DeltaN-Ect2 DH/PH) resulted in a dramatic reduction in transforming activity. Whereas DeltaN-Ect2 caused formation of lamellipodia, DeltaN-Ect2 DH/PH enhanced actin stress fiber formation, suggesting that C-terminal sequences influenced Ect2 Rho GTPase specificity. Consistent with this possibility, we determined that DeltaN-Ect2 DH/PH activated RhoA, but not Rac1 or Cdc42, whereas DeltaN-Ect2 DH/PH/C activated all three Rho GTPases in vivo. Taken together, these observations suggest that regions of Ect2 C-terminal to the DH domain alter the profile of Rho GTPases activated in vivo and consequently may contribute to the enhanced transforming activity of DeltaN-Ect2 DH/PH/C.
Characterization of double pulse-doped channel GaAs MESFETs (1993)

The fabrication and characterization of a double pulse-doped (DPD) GaAs MESFET grown by organometallic vapor phase epitaxy (OMVPE) are reported. The electron mobility of a DPD structure with a carrier concentration of 3*10/sup 18//cm/sup 3/ was 2000 cm/sup 2//V-s, which is about 20% higher than that of a pulse-doped (PD) structure. Implementing the DPD structure instead of the conventional PD structure as a GaAs MESFET channel, the drain breakdown voltage, current gain cutoff frequency, and maximum stable gain (MSG) increase. The maximum transconductance of 265 mS/mm at a drain current density of 600 mA/mm, a current gain cutoff frequency of 40 GHz, and an MSG of 11 dB at 18 GHz were obtained for a 0.3 mu m n/sup +/ self-aligned DPD GaAs MESFET. >
A Simplified Method for the Preparation of Ethynyl P-Tolyl Sulfone and Ethynyl Phenyl Sulfone (1994)

Abstract Silica gel mediated desilylation of aryl 2-(trimethylsilyl)ethynyl sulfones was found to greatly simplify the synthesis of the acetylenic sulfones, ethynyl ptolyl sulfone 1 and ethynyl phenyl sulfone 2. Each were easily prepared in good yield and high purity on a multigram scale from bis(trimethylsilyl)acetylene 3.
Integrating microsecond circuit switching into the data center (2013)

Recent proposals have employed optical circuit switching (OCS) to reduce the cost of data center networks. However, the relatively slow switching times (10--100 ms) assumed by these approaches, and the accompanying latencies of their control planes, has limited its use to only the largest data center networks with highly aggregated and constrained workloads. As faster switch technologies become available, designing a control plane capable of supporting them becomes a key challenge.
Improving passive radio‐frequency identification localisation precision with moving direction estimation‐based feature improvement (2014)

To achieve high localisation accuracy and precision is a fundamental requirement of passive radio‐frequency identification (RFID) location systems. Current passive RFID location systems mostly deploy a dense RFID tag distribution to give initial localisation accuracy and use advanced localisation algorithms to extract the reliable features for enhancing localisation precision. High quality features are capable of significantly improving precision of passive RFID localisation systems, but it is hardly achieved in practice because of the environmental noise interference and the variety of tag behaviours. A localisation approach by using moving direction estimation to improve the quality of extracted features is proposed for enhancing localisation precision of passive RFID location systems. This approach relies on a general distribution of false‐reading occurring probability function derived from experimental measurements. A feature adjustment scheme is introduced to correct these features regarding the estimation of moving direction of object. The experimental results demonstrate that the proposed localisation approach with feature improvement achieves higher precision for the state‐of‐the art deterministic localisation algorithm (Han et al . 2007) in passive RFID location systems.
Enzymic and Molecular Properties of Base-Plate Parts of Bacteriophage P22 (1976)

Using 14C-labeled Salmonella bacterial cells as the substrate, the enzymic and molecular properties of the base-plate parts of phage P22 were studied. The base-plate part consisted of a single protein species which cleaved extensively the O-antigen of Salmonella typhimurium, Salmonelly schotimuellerie and with somewhat slower rate that of Salmonella typhi, releasing oligosaccharide products with rhamnose at the reducing end. Much less cleavage was observed with a strain of S. typhimurium lysogenic for P22, and no significant reaction with Salmonella anatum, Salmonella newington and Salmonella minneapolis. The base-plate part enzyme was a very heat-stable protein and only 10–20% loss was observed after treatment at 85 °C for 5 min. The pH optimum of the enzyme was around 7.5, and the glycosidase activity was not influenced by the ionic strength (25–250 mM) of the medium or the presence of Mg2+. The molecular weight of the base-plate part was 320000 by sedimentation equilibrium. Dodecylsulphate-acrylamide gel electrophoresis revealed a single band of molecular weight 77000, indicating that a single base-plate part corresponds to a tetramer of identical subunits. Circular dichroism spectra of P22 base-plate parts showed a major contribution of β structure. The protein was rich in acidic amino acids, glycine and serine.