gebhart/patrepeval huggingface dataset:
Direct construction of a chromosome-specific<i>Not</i>I linking library from flow-sorted chromosomes (1989)

A linking library consists of genomic DNA fragments which contain a specific rare restriction enzyme site; such clones are very useful as probes in pulsed field gel electrophoresis and in mapping and cloning large regions of DNA. However, identifying those linking clones which map to a certain chromosomal region can be laborious. Therefore, we have developed a straightforward procedure for constructing a linking library directly from flow-sorted chromosomes. As a test of the approach, a NotI linking library was constructed from the chromosome 17 fraction of a flow-sort of human chromosomes, using only 70 ng of DNA. Thirteen of sixteen linking clones were mapped to chromosome 17, suggesting that the library is highly enriched for this chromosome. This method should be generally applicable to other chromosomes and enzymes as well.
Regulation of Inflammatory Cytokine Production by MKP‐5 in Macrophages (2015)

Mitogen-activated protein kinases (MAPKs) include p38 MAPKs, c-Jun N-terminal kinases (JNKs) and Extracellular signal-regulated kinases (ERKs), and they regulate many cell processes, such as cell division, differentiation and release of inflammatory mediators. MAPK activity is controlled by mitogen-activated protein kinase phosphatases (MKPs), a phosphatase family with 11 members. MKP-1 is the most studied member of MKP family, and it is one of the anti-inflammatory factors induced by glucocorticoids. Less is known about the other MAPK phosphatases although they hold a promise as anti-inflammatory drug targets. In this study, we investigated the effect of MKP-5 on MAPK phosphorylation and cytokine production in J774 mouse macrophages. We used MKP-5 siRNA and an MKP-5 inhibitor (AS077234-4) to modulate MKP-5 function. We found that MKP-5 controlled p38 MAPK phosphorylation, but not that of JNK or ERK. In addition, the production of IL-6 and TNF was suppressed by MKP-5 in macrophages. Our results introduce a novel concept that compounds able to enhance MKP-5 expression and/or activity hold anti-inflammatory potential, because MKP-5 down-regulates the release of inflammatory mediators by controlling p38 MAPK activity.
Preparation and utility of dianions from N-tert-butylthiophene-2-sulfonamide (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and utility of dianions from N-tert-butylthiophene-2-sulfonamideSamuel L. Graham and Thomas H. ScholzCite this: J. Org. Chem. 1991, 56, 13, 4260–4263Publication Date (Print):June 1, 1991Publication History Published online1 May 2002Published inissue 1 June 1991https://doi.org/10.1021/jo00013a034RIGHTS & PERMISSIONSArticle Views399Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (551 KB) Get e-Alerts Get e-Alerts
Let's Make It Clear: How Central Counterparties Save(d) the Day* (2010)

will certainly be featured in history books as one of the greatest financial failures so far, but it will also be recorded as yet another episode of the historically successful performance of clearing arrangements in ensuring the resiliency of markets. Recognizing the usefulness of safe and sound clearing and settlement procedures, the Federal Reserve has recently supported the attempt to shift the clearing of some contracts to a central counterparty. In this article, Cyril Monnet outlines the arguments in favor of central counterparty clearing, the economic rationale for trade clearing through a central counterparty, and some possible limits to the advantages of clearing trades through a central counterparty. Following the bankruptcy of Lehman Brothers in September 2008, market participants were worried that Lehman’s positions of more than $500 billion would take ages to unwind. Lehman’s creditors did not know whether they would be able to recover all of the funds from their Cyril Monnet is a senior economic advisor and economist in the Philadelphia
A high efficiency 500 W step-up Cuk converter (2002)

A design of a high efficiency, ultra low ripple 500 W converter is presented. The high efficiency and low ripple of the design is attributed to the use of the noninverting, step-up extension of the coupled-inductor Cuk converter. The comparable standard boost converter will have much more output ripple and lower efficiency. Detailed design descriptions, test data and comparison with the boost converter reveal the inherent efficiency and ripple advantages of the step-up coupled-inductor Cuk converter topology.
pH gated glucose responsive biomimetic single nanochannels (2012)

The development of pH gated glucose (Glu) biosensor is of great significance to human health. Herein, we have designed a pH gated Glu responsive biomimetic nanochannel, modified with 3-aminobenzeneboronic acid. The Glu responsive property can be regulated by pH which can switch nanochannels from the "on" to "off" state.
A Chip Correlation MMSE Receiver with Multipath Interference Correlative Timing for DS-CDMA systems (2005)

We describe a chip correlation MMSE receiver with multipath interference correlative timing (MICT) for downlink direct-sequence code-division multiple access (DS-CDMA) systems. The receiver can be implemented easily, and analysis shows that the proposed method can obtain a chip correlation matrix that is about 50-times faster than the covariance matrix in W-CDMA systems, and the bit error rate (BER) performance of the receiver is the same as that of conventional maximum likelihood detectors (MLDs), operating under ideal conditions. Further, computer simulations showed that the BER performance maintained a good performance even in fast fading environments.
High-dose chemotherapy and autologous haematopoietic stem cell rescue for children with high-risk neuroblastoma (2010)

Based on the currently available evidence, myeloablative therapy seems to be a good treatment option for children with high-risk neuroblastoma. It results in higher survival rates than conventional therapy, although possible higher levels of adverse effects should be kept in mind. A definitive conclusion regarding the effect of myeloablative therapy in different subgroups is not possible. This systematic review only allows a conclusion on the concept of myeloablative therapy; no conclusions can be made regarding the best treatment strategy. Future trials on the use of myeloablative therapy for high-risk neuroblastoma should focus on identifying the most optimal induction and/or myeloablative regimen. The best study design to answer these questions is a RCT. These RCTs should be performed in homogeneous study populations (for example, regarding stage of disease and patient age) and have a long-term follow up. Different risk groups should be taken into account.
Housing Design for a New RF Breast Coil Concept for Use in MRI Applications (2008)

MRI has proven to be a reliable means of breast cancer screening, superior in many ways to conventional screening methods. Reflecting the goal of the project sponsor, this project aims to design a mechanical housing concept that incorporates an existing multi-channel RF breast coil array concept. This included steps towards incorporating the necessary electrical and RF components in a design for a low profile, ergonomic housing as well as the development of design specifications that could be used by the sponsor and their engineers to eventually manufacture the breast coil and move it into the clinical MRI breast coil market.
Grasping of static and moving objects using a vision-based control approach (2002)

Recent work combining robotics with vision has emphasized an active vision paradigm where the system changes the pose of the camera to improve environmental knowledge or to establish and presence a desired relationship between the robot and objects in the environment. Much of this work has concentrated upon the active observation of objects by the robotic agent. In this paper we present extensions to the controlled active vision framework that focus upon the autonomous grasping of a moving or static object in the manipulator's workspace. Our work extends the capabilities of an eye-in-hand system beyond those as a "pointer" or a "camera orienter" to provide the flexibility required to robustly interact with the environment in the presence of uncertainty. The proposed work is experimentally verified using the Minnesota robotic visual tracker to automatically select object features, to derive estimates of unknown environmental parameters, and to supply a control vector based upon these estimates to guide the manipulator in the grasping of a moving or static object.
Practice Parameters for the Nonpharmacologic Treatment of Chronic Insomnia (1999)

Insomnia is the most common sleep complaint reported to physicians. Treatment has traditionally involved medication. Behavioral approaches have been available for decades, but lack of physician awareness and training, difficulty in obtaining reimbursements, and questions about efficacy have limited their use. These practice parameters review the current evidence with regards to a variety of nonpharmacologic treatments for insomnia. Using a companion paper which provides a background review, the available literature was analyzed. The evidence was graded by previously reported criteria of the American Academy of Sleep Medicine with references to American Psychological Association criteria. Treatments considered include: stimulus control, progressive muscle relaxation, paradoxical intention, biofeedback, sleep restriction, multicomponent cognitive behavioral therapy, sleep hygiene education, imagery training, and cognitive therapy. Improved experimental design has significantly advanced the process of evaluation of nonpharmacologic treatments for insomnia using guidelines outlined by the American Psychological Association (APA). Recommendations for individual therapies using the American Academy of Sleep Medicine recommendation levels for each are: Stimulus Control (Standard); Progressive Muscle Relaxation, Paradoxical Intention, and Biofeedback (Guidelines); Sleep Restriction, and Multicomponent Cognitive Behavioral Therapy (Options); Sleep Hygiene Education, Imagery Training, and Cognitive Therapy had insufficient evidence to be recommended as a single therapy. Optimal duration of therapy, who should perform the treatments, long term outcomes and safety concerns, and the effect of treatment on quality of life are questions in need of future research.
Modularization of XHTML (2001)

This Recommendation specifies an abstract modularization of XHTML and an implementation of the abstraction using XML Document Type Definitions (DTDs). This modularization provides a means for subsetting and extending XHTML, a feature needed for extending XHTML’s reach onto emerging platforms.
Preservation of Listeria monocytogenes and other pathogenic foodborne bacteria on silica gel (1993)

Journal Article Preservation of Listeria monocytogenes and other pathogenic foodborne bacteria on silica gel Get access L. K. Bagi, L. K. Bagi US Department of Agriculture, ARS, Eastern Regional Research Center, Microbial Food Safety Research Unit, 600 E. Mermaid Lane, Philadelphia. PA 19118, USA Search for other works by this author on: Oxford Academic Google Scholar R. L. Buchanan R. L. Buchanan US Department of Agriculture, ARS, Eastern Regional Research Center, Microbial Food Safety Research Unit, 600 E. Mermaid Lane, Philadelphia. PA 19118, USA Search for other works by this author on: Oxford Academic Google Scholar Letters in Applied Microbiology, Volume 17, Issue 1, 1 July 1993, Pages 37–39, https://doi.org/10.1111/j.1472-765X.1993.tb01430.x Published: 01 July 1993 Article history Received: 10 December 1992 Accepted: 27 January 1993 Published: 01 July 1993
Polymerase chain reaction (PCR) for detection of Mspl and Dral polymorphism at the THRB gene (1991)

Polymerase chain reaction (PCR) for detection of Mspl and Dral polymorphism at the THRB gene Get access P.S. Ganly, P.S. Ganly Medical Research Council Clinical Oncology and Radiotherapeutics Unit, Medical Research Council CentreHills Road, Cambridge, CB2 2QH, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar P.H. Rabbitts P.H. Rabbitts Medical Research Council Clinical Oncology and Radiotherapeutics Unit, Medical Research Council CentreHills Road, Cambridge, CB2 2QH, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 13, 11 July 1991, Page 3760, https://doi.org/10.1093/nar/19.13.3760 Published: 11 July 1991
Demonstration of a novel SCM based PON including a vitually-passive uplink element (1996)

This paper describes a WDM-SCM PON scheme with low speed user components which incorporates a novel quasi-passive coarse wavelength division multiplexing (CWDM) uplink concentrator. A proof of principle uplink system with 2 simultaneous 1 Gb/s SCM channels is shown to have very low transmission penalty.
Transmitter based inverse filters for MAI and ISI mitigation in a TDD/CDMA downlink (2003)

Recently, research has been focused on techniques that simplify the mobile receiver structure in a CDMA system by means of signal precoding. These methods assume knowledge of the channel at the transmitter which is feasible in a time division duplex (TDD) system where the channel is assumed to be the same on uplink and downlink. A novel algorithm based on prefiltering the spread signal in the base station is described. The prefilter taps are determined with an MMSE criterion and the necessary power constraint. We show that this technique increases the system capacity with a low computational cost compared with existing schemes. The portable unit's structure is reduced to the conventional receiver for a single user in an AWGN channel, a matched filter. Thus no channel estimation, equalisation or multiuser detection is required at the mobile. The theoretical analysis is supported with simulation results where the performance is compared to other recently published methods.
Reactions of titanium tetrachloride with aminoboranes / (1969)

With HCl-Synthesis and Characterization of \L ( CH 3
Titanium and Alloy Surfaces for Adhesive Bonding (1974)

Abstract The nature of the oxide on the surface of titanium and the Ti-6Al-4 V alloy after a number of recommended treatments has been examined by x-ray and electron diffraction. No evidence for material other than titania in its rutile form was obtained even though anatase and fluoride have been reported. The most efficient surface for adhesive bonding is a rough surface of the black oxide such as is produced by treatment of the metal in alkaline hydrogen peroxide.
Restriction of the TCR repertoire inhibits the development of memory T cells and prevents autoimmunity in lpr mice (1996)

The lpr mutation, a disruption of the fas gene, induces spontaneous autoimmunity characterized by high titers of autoantibodies, lymphadenopathy, autoreactive T cells, and early mortality. The mechanism of autoimmunity, however, remains unknown. The driving force for disease could result from the T cell recognition of autoantigen or, alternatively, an intrinsic T cell defect that promotes autoreactivity. We investigated the role of antigen-TCR interaction in the pathogenesis of lpr autoimmunity by transferring the DO-11.10 TCR beta-chain transgene (Vbeta8.2-Dbeta1.1-Jbeta1.1) to the MRL-lpr/lpr background producing the MRL-lprbeta strain. Our results show that the MRL-lpr beta transgenic strain has increased survival, lower titers of autoantibodies, and decreased lymphadenopathy compared with nontransgenic littermates. These beneficial effects were associated with decreased expansion of CD4+ T cells expressing memory phenotypes (CD44+, CD45RB-, and LECAM-) in the transgenic compared with nontransgenic strains. A role for impaired recognition of autoantigen by T cells expressing the TCR transgene was suggested by comparing the phenotypes of Vbeta8.2+ (transgene+) vs Vbeta8.2- (transgene-) CD4+ T cells within the transgenic mice. These experiments show that Vbeta8.2- T cells, which express endogenously rearranged TCR, are the major contributors to the expansion of memory T cells in the transgenic mice. In contrast, T cells with memory phenotypes expand similarly in both the Vbeta8.2+ and Vbeta8.2- subsets of nontransgenic mice. Based on these results, we hypothesize that TCR recognition of autoantigen is a major contributor to autoimmunity in lpr mice and that T cells expressing a memory phenotype are perpetrators of this process.
Diode-pumped microlasers for display applications (1998)

Laser displays have been investigated by engineers and scientists since shortly after the invention of the laser. The majority of these systems have been based on gas lasers or lamp-pumped solid-state lasers which are expensive, large in size, and require significant cooling systems. Due to these negative attributes, laser displays have been limited to applications which are not sensitive to size or cost. Recent advances in compact, air-cooled, diode-pumped, solid-state, visible microlasers have enabled the development of portable laser displays. Lasers are under development for both 'backlit' displays, where the lasers replace arc-lamps in an LCD/DMD projector, and 'direct-write' displays, where the image is formed by directly modulating and scanning the laser beam. Compact, multi-watt RGB laser modules have been demonstrated for use as 'light engines' in projection displays generating greater than 500 ANSI lumens. Advantages of microlaser-based displays include large color gamut, color accuracy, image uniformity, high resolution, large depth of focus, and low maintenance due to the long lifetime (greater than 10,000 hours) of the lasers. These advantages make them attractive for near term applications such as simulators, command and control centers, high end CAD workstation monitors, and longer term applications such as electronic cinema.
Vegetable Oil Deodorizer Distillate: A Rich Source of the Natural Bioactive Components (2016)

Deodorizer distillates are waste products of edible oil processing industries obtained during deodorization process of vegetable oils. It is very cheap source of several health beneficial components such as tocopherols, sterols, squalene as well as free fatty acids which have numerous industrial applications. These valuable components are being used in different foods, pharmaceutical formulations and cosmetics. Traditional sources of these useful components are vegetable oils, fruits, vegetables and nuts. Global need of these important components has been exceeded than their availability. The deodorizer distillates of various vegetable oils are considered to be a rich source of several valuable components. Present review will cover brief introduction of common processing stages involved in all vegetable oil processing, analytical methods for characterization of deodorizer distillates by instrumental techniques, importance and commercial value of deodorizer distillates. Future prospective of current field may leads to cost efficient processes and increased attention on the nutritional quality of deodorized oil and commercial applications of deodorizer distillates as well as their valuable components.
Acetylene .pi.-Coordination, Slippage to .sigma.-Coordination, and 1,2-Hydrogen Migration Taking Place on a Transition Metal. The case of a Ru(II) Complex as Studied by Experiment and ab Initio Molecular Orbital Simulations (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAcetylene .pi.-Coordination, Slippage to .sigma.-Coordination, and 1,2-Hydrogen Migration Taking Place on a Transition Metal. The case of a Ru(II) Complex as Studied by Experiment and ab Initio Molecular Orbital SimulationsYasuo Wakatsuki, Nobuaki Koga, Hiroshi Yamazaki, and Keiji MorokumaCite this: J. Am. Chem. Soc. 1994, 116, 18, 8105–8111Publication Date (Print):September 1, 1994Publication History Published online1 May 2002Published inissue 1 September 1994https://pubs.acs.org/doi/10.1021/ja00097a017https://doi.org/10.1021/ja00097a017research-articleACS PublicationsRequest reuse permissionsArticle Views941Altmetric-Citations169LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Discovery of a 2,4-Disubstituted Pyrrolo[1,2-<i>f</i>][1,2,4]triazine Inhibitor (BMS-754807) of Insulin-like Growth Factor Receptor (IGF-1R) Kinase in Clinical Development (2009)

This report describes the biological activity, characterization, and SAR leading to 9d (BMS-754807) a small molecule IGF-1R kinase inhibitor in clinical development.
Bench-to-bedside review: Delirium in ICU patients - importance of sleep deprivation (2009)

Delirium occurs frequently in critically ill patients and has been associated with both short-term and long-term consequences. Efforts to decrease delirium prevalence have been directed at identifying and modifying its risk factors. One potentially modifiable risk factor is sleep deprivation. Critically ill patients are known to experience poor sleep quality with severe sleep fragmentation and disruption of sleep architecture. Poor sleep while in the intensive care unit is one of the most common complaints of patients who survive critical illness. The relationship between delirium and sleep deprivation remains controversial. However, studies have demonstrated many similarities between the clinical and physiologic profiles of patients with delirium and sleep deprivation. This article aims to review the literature, the clinical and neurobiologic consequences of sleep deprivation, and the potential relationship between sleep deprivation and delirium in intensive care unit patients. Sleep deprivation may prove to be a modifiable risk factor for the development of delirium with important implications for the acute and long-term outcome of critically ill patients.