gebhart/patrepeval huggingface dataset:
Radical interception by carotenoids and effects on UV carcinogenesis (1998)

Studies employing time-resolved techniques have shown that beta-carotene, astaxanthin, and lycopene behave quite distinctly with respect to radical quenching and stability, lycopene being the least stable. These results are compatible with the relative effects of the various carotenoids on ultraviolet (UV)-mediated carcinogenesis in mice in which a statistically significant exacerbation by beta-carotene and astaxanthin, but not by lycopene, was observed. Interactions between these carotenoids and vitamin C and E radicals not only provide a chemical basis to explain the failure of beta-carotene to provide benefit in recent clinical trials but suggest that future carotenoid supplementation studies should proceed with caution until carotenoid interactions and radical repair mechanism(s) are elucidated.
Preparation and aromatization of poly‐1,3‐cyclohexadiene. II (1963)

Abstract Further work on the polymerization of 1,3‐cyclohexadiene by the action of Zieglertype catalysis and by cationic initiation has been carried out and polymers with an inherent viscosity of 0.1–0.19 have been obtained consistently. Dehydrogenation of the poly‐1,3‐cyclohexadiene by halogenation followed by pyrolysis has given good conversion to a polyphenyl which has some units which are incompletely aromatized. This polymer has very good heat stability but is not a useful polymer for plastic purposes.
A molecular chaperone, ClpA, functions like DnaK and DnaJ. (1994)

The two major molecular chaperone families that mediate ATP-dependent protein folding and refolding are the heat shock proteins Hsp60s (GroEL) and Hsp70s (DnaK). Clp proteins, like chaperones, are highly conserved, present in all organisms, and contain ATP and polypeptide binding sites. We discovered that ClpA, the ATPase component of the ATP-dependent ClpAP protease, is a molecular chaperone. ClpA performs the ATP-dependent chaperone function of DnaK and DnaJ in the in vitro activation of the plasmid P1 RepA replication initiator protein. RepA is activated by the conversion of dimers to monomers. We show that ClpA targets RepA for degradation by ClpP, demonstrating a direct link between the protein unfolding function of chaperones and proteolysis. In another chaperone assay, ClpA protects luciferase from irreversible heat inactivation but is unable to reactivate luciferase.
Epigenetic Regulation of <i>UBE3A</i> and Roles in Human Neurodevelopmental Disorders (2015)

The E3 ubiquitin ligase UBE3A, also known as E6-AP, has a multitude of ascribed functions and targets relevant to human health and disease. Epigenetic regulation of the UBE3A gene by parentally imprinted noncoding transcription within human chromosome 15q11.2-q13.3 is responsible for the maternal-specific effects of 15q11.2-q13.3 deletion or duplication disorders. Here, we review the evidence for diverse and emerging roles for UBE3A in the proteasome, synapse and nucleus in regulating protein stability and transcription as well as the current mechanistic understanding of UBE3A imprinting in neurons. Angelman and Dup15q syndromes as well as experimental models of these neurodevelopmental disorders are highlighted as improving understanding of UBE3A and its complex regulation for improving therapeutic strategies.
Radiosensitization of Hypoxic Mammalian Cells <i>in Vitro</i> by Some 5-substituted-4-nitroimidazoles (1981)

The efficiencies of various 5-substituted-4-nitroimidazoles as radiation sensitizers have been determined in hypoxic Chinese hamster cells irradiated in vitro. Compared with published data on the sensitizing properties of substituted 2-nitro- and 5-nitroimidazoles, some of the 4-nitro derivatives show unusually high sensitizing efficiencies defined as the concentrations required to give an enhancement ratio of 1.6. The equilibrium one-electron reduction potentials of the compounds (E17) were measured by a pulse radiolysis technique and the results show that although sensitizing efficiencies are unexpectedly high, based on considerations of electron affinity, they still increase with increasing values of E17. Enhancement ratios were determined in two V79 cell lines for combinations of one of these compounds (a 4-nitroimidazole containing the group SO2.O.phenyl in the 5-position, NSC 38087) with various concentrations of misonidazole. The sensitization observed suggests that the two compounds may be operating by different mechanisms.
Showroom Sample (1994)

Review| May 01 1994 Showroom Sample The Visual Politics of Hip Hop, Zeitgeist Theatre Experiments, New Orleans, Lousiana, January 28-29 Janet Sorensen Janet Sorensen Janet Sorensen is currently completing her Ph.D. in English at SUNY Buffalo and will begin teaching at Indiana University in the fall. Search for other works by this author on: This Site PubMed Google Scholar Afterimage (1994) 21 (10): 11–12. https://doi.org/10.1525/aft.1994.21.10.11 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Janet Sorensen; Showroom Sample. Afterimage 1 May 1994; 21 (10): 11–12. doi: https://doi.org/10.1525/aft.1994.21.10.11 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAfterimage Search This content is only available via PDF. © 1994 by The Regents of the University of California1994 Article PDF first page preview Close Modal You do not currently have access to this content.
Focused Differential Glycan Analysis with the Platform Antibody-assisted Lectin Profiling for Glycan-related Biomarker Verification (2008)

Protein glycosylation is a critical subject attracting increasing attention in the field of proteomics as it is expected to play a key role in the investigation of histological and diagnostic biomarkers. In this context, an enormous number of glycoproteins have now been nominated as disease-related biomarkers. However, there is no appropriate strategy in the current proteome platform to qualify such marker candidate molecules, which relates their specific expression to particular diseases. Here, we present a new practical system for focused differential glycan analysis in terms of antibody-assisted lectin profiling (ALP). In the developed procedure, (i) a target protein is enriched from clinic samples (e.g. tissue extracts, cell supernatants, or sera) by immunoprecipitation with a specific antibody recognizing a core protein moiety; (ii) the target glycoprotein is quantified by immunoblotting using the same antibody used in (i); and (iii) glycosylation difference is analyzed by means of antibody-overlay lectin microarray, an application technique of an emerging glycan profiling microarray. As model glycoproteins having either N-linked or O-linked glycans, prostate-specific antigen or podoplanin, respectively, were subjected to systematic ALP analysis. As a result, specific signals corresponding to the target glycoprotein glycans were obtained at a sub-picomole level with the aid of specific antibodies, whereby disease-specific or tissue-specific glycosylation changes could be observed in a rapid, reproducible, and high-throughput manner. Thus, the established system should provide a powerful pipeline in support of on-going efforts in glyco-biomarker discovery.
Transcription of DNA from the 70 <i>S</i> RNA of Rous Sarcoma Virus I. Identification of a Specific 4 <i>S</i> RNA Which Serves as Primer (1974)

The enzymatic transcription of DNA from the 70S RNA of Rous sarcoma virus (RSV) is initiated on the 3' terminus of a molecule of 4S RNA which is hydrogen bonded to the viral genome. We labeled this primer with radioactive deoxynucleotides, and demonstrated that its release from 70S RNA by thermal denaturation was accompanied by a reduction in the template activity of the viral RNA. Two-dimensional electrophoresis in polyacrylamide gels separated the 4S RNAs associated with the 70S RNA of RSV into approximately eight fractions, each of which appeared to contain a discrete species of tRNA. The RNA in one of these fractions served as the principal primer for initiation of DNA synthesis by both detergent-disrupted virions of RSV and purified RNA-directed DNA polymerase with RSV 70S RNA as template.
Pharmacological Modulation of Anti-Tumor Immunity Induced by Oncolytic Viruses (2014)

Oncolytic viruses (OVs) not only kill cancer cells by direct lysis but also generate a significant anti-tumor immune response that allows for prolonged cancer control and in some cases cures. How to best stimulate this effect is a subject of intense investigation in the OV field. While pharmacological manipulation of the cellular innate anti-viral immune response has been shown by several groups to improve viral oncolysis and spread, it is increasingly clear that pharmacological agents can also impact the anti-tumor immune response generated by OVs and related tumor vaccination strategies. This review covers recent progress in using pharmacological agents to improve the activity of OVs and their ability to generate robust anti-tumor immune responses.
Identification of enhancer-like elements in human IFN-gamma genomic DNA. (1990)

We have previously shown that transfection of a plasmid clone containing full length human IFN-gamma genomic DNA into a murine T-lymphoblastoid line is followed by basal expression of the transfected gene, with increased transcription occurring upon stimulation of the cells with either phorbol ester or IL-2. In addition, upon transfection of this DNA into murine fibroblasts, high level constitutive transcription was observed. In contrast to the results obtained under tissue culture conditions, introduction of the same DNA into the mouse germline resulted in tissue-specific expression of the transgene. We now report identification of a region 500-bp 5' of the human IFN-gamma TATAA box that has strong, PMA-inducible, enhancer-like activity when linked to a reporter gene (CAT) and transfected into a murine T cell line. However, when the same region of IFN-gamma genomic DNA was introduced into NIH-3T3 cells, no enhancer activity was detected either in the presence or absence of PMA. We have further found that an intronic region of the IFN-gamma genomic DNA (nucleotides 405-674) also contains enhancer activity that is functional in either fibroblasts or T cells. Enhancer activity of the intronic region is also PMA-inducible in the mouse T cells but constitutive in fibroblasts. Collectively, our observations suggest that control of human IFN-gamma gene expression is complex, involving noncontiguous regulatory domains in both 5' flanking and intronic regions of that gene.
Race logic architecture (RALA): a novel logic concept using the race scheme of input variables (2002)

A novel logic concept, Race Logic Architecture (RALA), is proposed. RALA is a new logic operation architecture in that the racing between input variables along the interconnection lines functions as an active logic element instead of logic gates, while the logic gates play a simple passive role. Logic operations of RALA are based on wired-OR that utilizes shared space and serial-AND that utilizes the triggering sequence of input variables. With these two concepts, RALA can implement arbitrary Boolean operations. Various kinds of combinational circuits are simulated and compared with RALAs. RALA shows the best performance in delay time, area, and power product results. A 64-bit carry-look-ahead adder with RALA is fabricated by 0.25-/spl mu/m CMOS technology to verify its feasibility and functionality. The area of the adder is 800 /spl mu/m/spl times/150 /spl mu/m, and the delay time from the clock to Sum31 measured 0.9 ns.
Physical activity from rehabilitation to independent community function: the role of physical activity in handicapping conditions. (1991)

All ingredients are there--philosophies and principles, models and examples, competent and committed professionals, ready and willing agencies and organizations, and anxious-to-get-involved persons with handicapping conditions. The time has never been so right to go forward and onward to the heights with sport, recreation, and physical activity to produce lives worth living by persons with handicapping conditions. George Washington warned: "We ought not to look back unless it is to derive useful lessons from past errors, and for the purpose of profiting by dearly bought experience." In this case some might advise not to look back at all, because pessimists and disbelievers may be trying to overtake optimists and believers. As Henry David Thoreau stated: "If you build castles in the air, your work need not be lost; that is where they should be. Now put the foundations under them." Physical activity, recreation, and sport are important in physical, mental, emotional, and social adjustment of individuals with handicapping conditions. In many cases, active participation in such activities provides more effective and efficient rehabilitation and therapy than formal rehabilitation and therapeutic modalities and approaches. Throughout history some pioneers striving to introduce and use such techniques and approaches have been rebuffed and rejected by many facets of the medical establishment. Often, progress has come from highly committed advocates from various professional specializations with interests in activity programs for their populations. Although progress continues to be made, much greater recognition by and involvement of the medical profession are needed from the classrooms in medical schools, through all internships and residencies, to hospitals and clinics of all types, and among all medical branches and specialties.
Role of renin angiotensin system in pathogenesis of glomerulonephritis (2005)

近年, アンジオテンシンII (Ang II) の多面的な細胞活性が注目されている. Ang IIは古典的な解釈を超え, その多様性に富む細胞活性化を通して様々な疾患の病態に影響を与えることが明らかになってきた. また, レニン・アンジオテンシン系 (RAS) の作用の一部は, Ang IIが産生される場所に依存している可能性があり, 局所のRASの重要性が議論されている. 糸球体腎炎の病態におけるRASの役割は, 進展および発症という2点に整理される. 進展因子としては, Ang IIによるTGFβ発現増強などを介した糸球体硬化の機序に関わる点と, 尿細管間質の線維化を惹起する主要因子である尿蛋白の調節因子という点が考えられる. 一方でRASは, 白血球浸潤機序に深く関わることも判明し, 炎症の惹起因子として働いていることも考えられている. 原因を問わず糸球体障害により活性化された局所のRASは, 免疫担当細胞の浸潤を誘導することが報告されている. このAng IIを介した白血球浸潤は, 尿細管間質病変と同様に障害に付随する非特異的な炎症性の応答である. 最近われわれは, 糸球体に沈着する免疫複合体の一部は, 沈着すること自体で糸球体局所のRASを活性化し, 抗原特異的T細胞 (Th1タイプ) の糸球体内への浸潤を強力に誘導し, 糸球体腎炎の発症の感受性や予後を規定することを確認した. Ang IIはメサンギウム細胞上のAT1レセプターを介してTh1特異的なケモカインの産生を増加させることから, これがT細胞依存性糸球体腎炎の機序の一つと考えられた. このようにRASは, 免疫現象の一部として腎炎の発症に関わる可能性も考えられ, RAS抑制薬を腎炎に投与する意義は想像以上に大きいかもしれない.
A low power CMOS UWB pulse generator (2005)

The paper presents a low power CMOS pulse generator design for impulse based UWB applications. The basic structure of the design involves a variable length rectangle pulse generator, a CMOS quasi-Gaussian pulse shaping filter and a small monopole antenna model. Simulation results show the pulse after the antenna is compliant with the FCC's spectrum mask for 0~960 MHz band and has a power consumption of 234 muW at the pulse repetition frequency of 100 MHz. The CMOS circuit used to create the pulse generator is simple and lends itself to a low-power, single-chip UWB transmitter solution
A graphical environment for the design of concurrent real-time systems (1997)

Concurrent real-time systems are among the most difficult systems to design because of the many possible interleavings of events and because of the timing requirements that must be satisfied. We have developed a graphical environment based on Real-Time Graphical Interval Logic (RTGIL) for specifying and reasoning about the designs of concurrent real-time systems. Specifications in the logic have an intuitive graphical representation that resembles the timing diagrams drawn by software and hardware engineers, with real-time constraints that bound the durations of intervals. The syntax-directed editor of the RTGIL environment enables the user to compose and edit graphical formulas on a workstation display; the automated theorem prover mechanically checks the validity of proofs in the logic; and the database and proof manager tracks proof dependencies and allows formulas to be stored and retrieved. This article describes the logic, methodology, and tools that comprise the prototype RTGIL environment and illustrates the use of the environment with an example application.
Pathogenesis of Liver Injury (2007)

Acute and chronic liver injuries are everyday occurrences in clinical practice. Indeed, monitoring, surveillance, and screening for liver disease by measuring serum aminotransferase values are standards of care. Despite the widespread recognition of the importance of liver injury in human health, the mechanisms underlying liver injury remain complex and incompletely understood. However, considerable progress has been made recently in understanding the pathogenesis of liver injury. This emerging information has spurred several biotech and large pharmaceutical companies to develop and test hepatoprotective and antifibrogenic agents. Given the rapid expansion of our knowledge on the pathogenesis of liver injury, which is being translated into potential therapeutic approaches, it is both timely and topical to review the pathogenic mechanisms of liver injury. Therefore, this issue of Seminars in Liver Disease is devoted to this topic.
Enzymes of phospholipid metabolism in rat pancreatic islets: Subcellular distribution and the effect of glucose and calcium (1986)

The effect of glucose and calcium on the activities of the phosphatidylinositol cycle enzymes, CDP-diglyceride inositol transferase, diacylglycerokinase, and lysophosphatidylcholine 2-acyltransferase in rat pancreatic islets was studied. Calcium inhibited the activity of CDP-diglyceride inositol transferase but had no effect on lysophosphatidylcholine 2-acyltransferase and diacylglycerokinase activities. Upon preincubation of islets in a concentration of glucose known to stimulate insulin release, the activity of lysophosphatidylcholine 2-acyltransferase, but not that of diacylglycerokinase or the CDP-diglyceride inositol transferase, was stimulated. Subcellular fractionation of pancreatic islets showed that secretory granule membranes were enriched in CDP-diglyceride inositol transferase, whereas lysophosphatidylcholine 2-acyltransferase activity was highest in the microsomal membranes. The activation of 2-acyltransferase by incubating islets in insulinotropic glucose, and the calcium sensitivity of CDP-diglyceride inositol transferase, suggest that these enzymes may have roles in regulation of insulin secretion.
Two-dimensional equalization: theory and applications to high density magnetic recording (1994)

Two dimensional, separable signal models are proposed for modeling the effects of intersymbol and inter-track interference in high-density magnetic storage media. The optimum (minimum mean-squared error) linear and decision feedback equalizers based on these models are developed. The use of a two dimensional LMS algorithm for adaptive updating of the equalizer coefficients is discussed and analytical results presented. Finally, simulation evidence is provided to predict the performance improvements achievable based on these models vis-a-vis one dimensional equalization.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Bandwidth tracing arbitration algorithm for mixed-clock SoC with dynamic priority adaptation (2004)

As the processing capabilities and operating frequency of embedded system are growing, so is the needed data bandwidth to fully utilize the processing capability. The ability to transfer huge amount of data between the embedded core and external devices is required for efficient system operation. In this paper, the data communication architecture for the mixed-clock system is proposed. The dynamic priority adaptation algorithm for bus arbitration is proposed for bandwidth guarantee. The communication architecture that incorporates the proposed arbitration algorithm adapts the priority of communication components dynamically based on the information from FIFO. The experiments show that the measured bandwidth of each component traces the required bandwidth well compared to the other arbitration algorithms.
A new approach to measuring transepithelial potentials in the bovine lens reveals a chloride‐dependent component (1994)

A new approach was used to measure anterior and posterior potentials and resistances of the bovine lens. Segments of the anterior, central and posterior lens were studied by mounting a disc of tissue from either the front or the back of the lens in a double chamber. At 35 degrees C the isolated anterior epithelial preparation exhibited a high potential of 9 mV and a low resistance of 279 omega cm2. The lens epithelium can therefore be classified as a 'leaky' or low resistance epithelium. Amiloride, benzamil and furosemide (frusemide) all reduced the anterior transepithelial potential and increased the resistance, amphotericin B (aqueous side) abolished the potential, whereas SITS (4-acetamido-4'-isothiocyanatostilbene-2,2'-disulphonic acid) had no effect. The posterior lens preparation was found to have a 'transepithelial' of -4 mV. This potential and a proportion of the anterior potential were not sensitive to ouabain, amiloride, furosemide, Na+ removal or raised K+, but were abolished by lowering [Cl-] and reduced by the Cl- channel blocker NPPB. We conclude that chloride plays a significant role in the maintenance of transepithelial potentials at both the anterior and posterior surfaces of the lens.
A polarimetric line-of-sight channel model for MIMO satellite communications (2013)

Spatial multiple-input multiple-output (MIMO) offers increased rate and/or reliability in rich scattering environments such as that of a multipath non-line-of-sight (NLOS) channel. In line-of-sight (LOS) dominated conditions, spatial diversity is reduced. Dual polarisation signalling has been shown to successfully exploit polarisation diversity when LOS is dominant. For satellite systems, such state-of-the-art dual-polarisation techniques do not currently account for the 3D geometry of the link, and specifically the polarisation mismatch from misalignment of the antenna elements. Performance loss due to the mismatch in antenna orientation can be overcome by using 3D polarimetric antennas at both the transmitter and receiver. In this paper, we present a novel polarimetric LOS MIMO channel model by combining satellite geometry, the Friis equation and polarisation mismatch. Our emphasis is on polarisation mismatch and how this varies over the field-of-view (FOV). Our model offers the opportunity for 3D polarimetric signalling techniques to deliver enhanced and more consistent link performance over the FOV.
X‐linked vacuolated myopathy: Membrane attack complex deposition on muscle fiber membranes with calcium accumulation on sarcolemma (1997)

Cytochemical localization of calcium on muscle fibers from patients with X-linked vacuolated myopathy demonstrated strong sarcolemmal or vacuolar calcium deposits in histologically abnormal muscle fibers. All membrane attack complex-positive fibers demonstrated such calcium accumulation. Total content of calcium was significantly elevated in muscles from patients with X-linked vacuolated myopathy compared to control muscles. Taken together, these results suggest that calcium accumulation on sarcolemma could be probably secondary to membrane attack complex deposition on the cell surface.