gebhart/patrepeval huggingface dataset:
Multiple forms of the human tyrosine phosphatase RPTP alpha. Isozymes and differences in glycosylation. (1994)

Among all the receptor-linked protein-tyrosine-phosphatase RPTP alpha clones described from mammalian tissues, one differed in that it encoded a 9-amino-acid insert 3 residues upstream from the transmembrane segment (Kaplan, R., Morse, B., Huebner, K., Croce, C., Howk, R. Ravera, M., Ricca, G., Jaye, M., and Schlessinger, J. (1990) Proc. Natl. Acad. Sci. U. S. A. 87, 7000-7004). Using the polymerase chain reaction technique, simultaneous expression of both isoforms was demonstrated in human T-cell and vascular smooth muscle libraries, as well as in the A431 human epidermal cancer cell line. Following transient expression in COS-1 cells, each isoform gave rise to two proteins of 100 and 130 kDa, respectively. Endoglycosidase treatment showed that the 100-kDa species corresponded to a molecule exclusively glycosylated on N-residues, whereas the 130-kDa species contained both, N- and O-linked carbohydrates. Pulse-chase experiments demonstrated that the smaller RPTP alpha protein is a precursor of the larger one. A high affinity antibody was generated that recognizes the immature protein only; however, both proteins can be detected by Western blot analysis after a simple chemical hydrolysis. Following Superose 12 chromatography, the 100- and 130-kDa species of RPTP alpha emerged as 200- and 340-kDa proteins, respectively. Both species exhibited similar enzymatic activities as determined with a peptide substrate in immunoprecipitates.
Olefin hydrogenation over zeolite H-ZSM-5. (1986)

Conversion of synthesis gas to hydrocarbons was carried out in a two-stage system consisting of a methanol synthesis reactor followed by a hydrocarbon-forming reactor containg H-ZSM-5 zeolite. It was found that the H-ZSM-5 zeolite catalyst was active in olefin hydrogenation.
Interstrand cross-linking of duplex DNA by nitrous acid: covalent structure of the dG-to-dG cross-link at the sequence 5'-CG (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInterstrand cross-linking of duplex DNA by nitrous acid: covalent structure of the dG-to-dG cross-link at the sequence 5'-CGJames J. Kirchner, Snorri T. Sigurdsson, and Paul B. HopkinsCite this: J. Am. Chem. Soc. 1992, 114, 11, 4021–4027Publication Date (Print):May 1, 1992Publication History Published online1 May 2002Published inissue 1 May 1992https://pubs.acs.org/doi/10.1021/ja00037a001https://doi.org/10.1021/ja00037a001research-articleACS PublicationsRequest reuse permissionsArticle Views271Altmetric-Citations68LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Adeno-Associated Virus-Mediated Gene Transfer in Hematopoietic Stem/Progenitor Cells as a Therapeutic Tool (2006)

Hematopoietic stem cells (HSCs) have unique properties of self-renewal, differentiation and proliferation. HSCs are easily accessible, and can be readily delivered back to patients by autologous transplantation, which renders them as attractive targets for ex vivo gene therapy. The adeno-associated virus (AAV) vectors have to date not been associated with any malignant disease, and have gained attention as a potentially safer alternative to the more commonly used retroviral vectors for HSC gene therapy. Although conflicting data exist with regard to HSC transduction by AAV vectors, in this review, we provide an overview of AAV-mediated HSC gene transfer - obstacles as well strategies to improve the transduction efficiency - and the potential use of AAV vectors for gene therapy of human diseases involving HSCs.
High quality alias free image rotation (2002)

This paper presents new algorithms for the rotation of images. The primary design criteria for these algorithms is very high quality. Common methods for image rotation, including convolutional and separable approaches, are examined and shown to exhibit significant high frequency aliasing problems. A new resampling filter design methodology is presented which minimizes the problem for conventional convolution-based image rotation. The paper also presents a new separable image rotation algorithm which exhibits improved performance in term of reduction in artifacts and an efficient O(N/sup 2/logN) running time.
A pluggable service-to-service communication mechanism for home multimedia networks (2002)

This paper proposes a pluggable service-to-service (S2S) communication mechanism in a middleware for home networks, called Virtual Networked Appliance (VNA) architecture. In the architecture, service description method and the plug-gable S2S communication mechanism are separated in an orthogonal way. Through the separation, VNA architecture solved problems of home networks on which users have to operate multiple heterogeneous middleware technologies simultaneously: middleware fragmentation problem, due to complexity of realizing heterogeneous services on one middle-ware technology: aspect realization violation problem. The pluggable S2S communication mechanism provides service programmers with a simple aspect representation method to define a service-specific protocol concern apart from the service's implementation. It also provides off-the-shelf protocol modules of such well-known communication protocols as RTP, RTSP, HTTP, and SMTP for an inter-service communication, and dynamically loads them based on the aspects defined by the programmer. This reduces the complexity of implementing heterogeneous services on the VNA architecture, thereby addressing the problems. In this paper, we first clarify the two problems. Then, we describe the proposed mechanism with an overview of the middleware architecture referring to a composite service: "Follow-You-and-Me Video."
The Inhibition of Coal Oxidation and Self Heating by Commercial Dust Suppressants (1987)

The ability of some commonly used dust suppressants to inhibit coal oxidation has been determined from calorimetry. An agglomerating agent, consisting primarily of an emulsified oil, was found to be the most effective inhibitor, resulting in a 50% reduction in the heating rate of the coal. This reduction has been subslantialed in stockpiling trials. The concentration of emulsifier used in this reagent was found to significantly affect the extent to which it inhibited the oxidation reaction. This is believed to be due to changes in the relative magnitudes of the rates of pore penetration and surface spreading, of the oil, with increasing emulsifier concentration.
Prognostics of aircraft bleed valves using a SVM classification algorithm (2012)

Non planned maintenance in aircraft systems is usually associated with high costs. It is believed that at least part of these costs can be avoided if adequate system prognosis programs are used. The aim of these programs is to evaluate the current state of an aircraft component based on the available system data (e.g., flight and maintenance data) and to estimate the future performance and the remaining useful life of this component. Several algorithms can be used for this purpose. This paper proposes a method of performing prognostics on aircraft component based a binary classification Support Vector Machine (SVM) Classification algorithm. The algorithm is used to classify each flight according to a fault pattern which the algorithm was trained to recognize. Flight data and maintenance logs were used to generate the training and testing datasets. In this case study, for each flight, a number of characteristics were extracted from parameters of the aircraft Air Management System. From the classification results a degradation index is created to serve as an aid to better plan the aircraft maintenance. An advantage of the proposed method is that it does not require a deep knowledge on the system. Furthermore, it does not need a large amount of input-output samples to be trained. The method should be easily extended to other aircraft systems as long as enough flight data and maintenance logs are available.
Anticonvulsant Therapy for Mood Disorders (1994)

Lithium therapy for bipolar illness is well accepted, despite some difficulties with its use. The administration of anticonvulsants has increased over the past 20 years. This paper outlines advantages and problems associated with anticonvulsant therapy for mood disorders.
Health‐related quality of life after radical cystectomy for bladder cancer: a comparison of ileal conduit and orthotopic bladder replacement (2002)

Objective To compare the health‐related quality of life (HRQoL) after radical cystectomy in patients with an ileal conduit or an orthotopic neobladder. Patients and methods The study included 85 men who underwent radical cystectomy for bladder cancer, comprising 48 with an orthotopic neobladder (26 with an ileal and 22 with a colon neobladder) and 37 with an ileal conduit. HRQoL was evaluated using the Short Form‐36 survey containing 36 questions assessing eight aspects, including physical functioning, role‐physical functioning, bodily pain, general health, vitality, social functioning, role‐emotional functioning and mental health. Results The mean follow‐up periods for patients with a neobladder (ileal and sigmoid) and with an ileal conduit was 45.9 (38.2 and 53.1, respectively) and 130.9 months, respectively. Scale scores were not affected by the duration of follow‐up in either group. There was no significant difference in any scale scores between the neobladder and ileal conduit groups. However, general health and social functioning in both the neobladder and ileal conduit groups appeared to be significantly lower than those in the general population in the USA. Furthermore, patients with a colon neobladder had a significantly higher score for role‐emotional functioning than those with an ileal neobladder, while there was no significant difference in the remaining seven scores between patients with ileal and colon neobladders. Conclusions Six of the eight scales of HRQoL were favourable in both patients with a neobladder or an ileal conduit, and there was no significant difference between these groups. In addition, the HRQoL of patients with an orthotopic neobladder (except for role‐emotional functioning) was unaffected by the segment of the intestine used for neobladder construction. Therefore, patients with both types of urinary diversion were generally satisfied with their overall health and quality of life.
Adulticidal Activity of Five Essential Oils against Culex pipiens quinquefasciatus (2005)

The aim of this study is to observe the adulticidal activity of five essential oils against the mosquito Culex pipiens quinquefasciatus. Fumigating adulticidal activity was investigated by airtight fumigation in conical flasks. The result showed that the toxic effect of the five essential oils varied with the period of fumigation. Rutaceae oil was the most toxic of the five. Carvacryl oil had the shortest adulticidal time (6.087 min). The chemical components of rutaceae oil were analyzed by GC/MS. The major components were α-citral (33.50%) and citral (35.77%). Citral showed marked adulticidal activity in a short-term fumigation. All five essential oils had considerable adulticidal effects on Cx. pipiens quinquefasciatus. © Pesticide Science Society of Japan
Regulation of cell fate determination in plants (2014)

EDITORIAL article Front. Plant Sci., 28 July 2014Sec. Plant Cell Biology https://doi.org/10.3389/fpls.2014.00368
Amino acid sequence of bovine spleen cathepsin B. (1988)

The complete amino acid sequence of bovine spleen cathepsin B was determined by manual and automatic Edman degradation of fragments prepared by proteolytic or chemical digestion of the enzyme. The single-chain form of the enzyme consists of 253 amino acid residues and its Mr is 27,468 (carbohydrate moiety not included). The light chain (residues 1-47) and the heavy chain (residues 50-253) of the enzyme are linked by the sequence -Gly-Arg (residues 48 and 49) in the single-chain form. Bovine spleen cathepsin B shows 80% sequence homology with cathepsins B from other species. An outstanding feature of bovine spleen cathepsin B not observed with the other cathepsins B is the presence of two additional half-cystine residues.
Root Coverage of Advanced Gingival Recession: A Comparative Study Between Acellular Dermal Matrix Allograft and Subepithelial Connective Tissue Grafts (2002)

Recession defects may be covered using ADMA or CTG, with no practical difference. However, CTG results in significantly greater gain of keratinized gingiva.
SPECIFIC MAPPING OF DISEASE RESISTANCE GENES IN TETRAPLOID CUT ROSES (2009)

Control of fungal diseases is a major constraint of cut-rose cultivation in greenhouses and in transportation around the world. Therefore, development of resistant cultivars is a promising way to reduce the use of chemicals required for controlling the diseases. Genetic analyses and breeding for resistance, however, are hampered by the high degree of heterozygosity and the polyploid nature of cultivated roses. Nucleotide-binding site (NBS) profiling of Van der Linden et al. (2004) was used as a tool enabling a more directed way of studying the genetics of resistance to pathogens responsible for diseases such as powdery mildew. NBS profiling is a multiplex screening technique, producing amplified resistance gene (R-gene) and resistance gene analogue (RGA) fragments by using degenerated primers based on the conserved motifs present in the NBS domain of resistance genes. Since RGAs are abundantly distributed and highly polymorphic within the plant genome, NBS profiling generates multiple markers of putative resistance genes. Twelve NBS degenerated primer/ restriction enzyme combinations were used to genotype the whole rose tetraploid K5 population (Yan, 2005) and its parents. To generate RGA profiles, the restriction enzymes: AluI, HaeIII, Mse and RsaI were used in combination with degenerated primers NBS1, NBS3, and NBS5a6. The profiles were dominantly scored resulting in 106 polymorphic RGA markers which segregated in a 1:1 or 3:1 ratio. Uni-and bi-parental simplex markers will be mapped on the two available AFLP/SSR K5 maps (Yan, 2005) with Joinmap 4.0. The resulting parental tetraploid maps will be used to dissect the genetic variation for resistance to powdery mildew resistance. Additional Rosaceae SSRs mentioned in the literature are currently tested on the K5 population to obtain allelic bridges between the tetraploid and diploid genetic maps in rose and related species in order to align them. These bridges will improve cross-ploidy comparisons in roses in order to strengthen cut rose breeding
Comparison of N<sub>2</sub> and NH<sub>3</sub> Plasma Passivation Effects on Polycrystalline Silicon Thin-Film Transistors (1998)

The passivation effects of pure N 2 plasma on polycrystalline silicon (poly-Si) thin-film transistors (TFTs) have been studied and compared with those of NH 3 plasma. We found that, similar to the recently-proposed NH 3 plasma passivation, N 2 plasma passivation can also effectively improve device parameters such as field effect mobility, threshold voltage, subthreshold swing, off current, and on/off current ratio. While the improvement of NH 3 -passivated devices can be attributed to both hydrogen and nitrogen passivation effects, nitrogen passivation alone is mainly responsible for the improvement of N 2 -passivated devices. In addition, we found that N 2 -passivated devices exhibit much better hot-carrier reliability than NH 3 -passivated devices. The better hot-carrier reliability of N 2 -passivated devices can be explained by the formation of strong Si–N bonds which are difficult to break, while effectively eliminating the weaker N–H bonds, which are inevitable in NH 3 -passivated devices. The results of a Fourier transform infrared (FTIR) spectrum analysis performed in this study are consistent with this proposition.
Aqueous-Based Polymeric Dispersion: Face-Centered Cubic Design for the Development of Atenolol Gastrointestinal Therapeutic System (1998)

The main objective of the study was to use the statistical response surface methodology to evaluate the effect of formulation variables and their interaction on drug release in atenolol gastrointestinal therapeutic system (GITS) development coated with cellulose acetate (CA) pseudolatex system. A three-factor, three-level, face-centered design was employed to evaluate the quadratic response surface, and to achieve the desired response in atenolol delivery. Coating was achieved by spraying plasticized CA pseudolatex on atenolol bilayered tablets in a Uni-Glatt fluidized-bed coater. The independent variables were orifice size (X1), percent coating weight gain (X2) and Carbopol 934P (X3). The response variable (Y1) was time for 50% (t50%) drug dissolution with a constraint on cumulative percent released in 24 hr (Y2). The polynomial equation obtained was Y1 = 61.88 - 3779.8X1 + 1.89X2 - 3.95X3 - 266.87 X1X2 - 1675X1X3 + 0.07X2X3 + 215123X1(2) + 0.06X2(2) + 0.10X3(2). Contour and response plots were used to relate the independent and dependent variables. The optimization procedure predicted a t50% of 10.83 hr with 92% atenolol release in 24 hr when orifice size (X1), percent coating weight gain (X2), and Carbopol 934P (X3) were 0.014 in., 13%, and 20 mg, respectively. Preparation and testing of optimized formulation showed a good correlation between the predicted and observed values.
Cross-domain approximate string matching (2003)

Approximate string matching is an important paradigm in domains ranging from speech recognition to information retrieval and molecular biology. We introduce a new formalism for a class of applications that takes two strings as input, each specified in terms of a particular domain, and performs a comparison motivated by constraints derived from a third, possibly different domain. This issue arises, for example, when searching multimedia databases built using imperfect recognition technologies (e.g., speech, optical character, and handwriting recognition). We present a polynomial time algorithm for solving the problem, and describe several variations that can also be solved efficiently.
Stat5 promotes metastatic behavior of human prostate cancer cells in vitro and in vivo (2010)

There are no effective therapies for disseminated prostate cancer. Constitutive activation of Stat5 in prostate cancer is associated with cancer lesions of high histological grade. We have shown that Stat5 is activated in 61% of distant metastases of clinical prostate cancer. Active Stat5 increased metastases formation of prostate cancer cells in nude mice by 11-fold in an experimental metastases assay. Active Stat5 promoted migration and invasion of prostate cancer cells, and induced rearrangement of the microtubule network. Active Stat5 expression was associated with decreased cell surface E-cadherin levels, while heterotypic adhesion of prostate cancer cells to endothelial cells was stimulated by active Stat5. Activation of Stat5 and Stat5-induced binding of prostate cancer cells to endothelial cells were decreased by inhibition of Src but not of Jak2. Gene expression profiling indicated that 21% of Stat5-regulated genes in prostate cancer cells were related to metastases, while 7.9% were related to proliferation and 3.9% to apoptosis. The work presented here provides the first evidence of Stat5 involvement in the induction of metastatic behavior of human prostate cancer cells in vitro and in vivo. Stat5 may provide a therapeutic target protein for disseminated prostate cancer.
Inhibition of TLR2 promotes graft function in a murine model of renal transplant ischemia‐reperfusion injury (2011)

Toll-like receptors (TLRs) are important molecules involved in the activation of innate and subsequent development of adaptive immunity. TLRs are ligated by exogenous ligands from pathogens and by endogenous ligands released in inflammatory diseases. Activation of TLR leads to activation of NF-κB and release of proinflammatory cytokines, such as IL-6 and TNF-α. TLRs play an important role in the pathogenesis of renal diseases. Increased expression of TLRs have been associated with ischemic kidney damage, acute kidney injury, end-stage renal failure, acute renal transplant rejection, and delayed allograft function. OPN301 is a mouse anti-human TLR2 antibody that cross-reacts with mouse TLR2. We show that inhibition of TLR2 promotes graft function in an isograft model of renal transplantation. Recipient mice were treated intravenously with OPN301 before reperfusion of the transplanted kidney that had been subjected to 30 min of cold ischemia. After 5 d, the residual native kidney was removed, and renal transplant function was assessed 24 h later by measurement of blood urea nitrogen. Renal function in both saline- and isotype-treated mice was similar, with significant improvement in OPN301-treated mice (isotype-treated vs. OPN301-treated: 33.9±3.2 vs. 19.8±1.9 μM; P<0.01). The histopathological appearance corresponded with renal functional results. In OPN301-treated recipients, renal structure was well preserved, whereas in the saline-treated group, tubular injury was severe, with marked tubular thinning, epithelial shedding, cast formation and necrosis. Inhibition of TLR2 also leads to a decrease in C3d deposition, although it is unclear whether this is due directly to TLR2 inhibition or a decrease in renal inflammation. This study shows that inhibition of TLR2 with a therapeutic agent (OPN301) provides significant protection from ischemia/reperfusion injury in a model of kidney transplantation.
The alchemy screen-space ambient obscurance algorithm (2011)

Ambient obscurance (AO) produces perceptually important illumination effects such as darkened corners, cracks, and wrinkles; proximity darkening; and contact shadows. We present the AO algorithm from the Alchemy engine used at Vicarious Visions in commercial games. It is based on a new derivation of screen-space obscurance for robustness, and the insight that a falloff function can cancel terms in a visibility integral to favor efficient operations. Alchemy creates contact shadows that conform to surfaces, captures obscurance from geometry of varying scale, and provides four intuitive appearance parameters: world-space radius and bias, and aesthetic intensity and contrast.
Comparison of human B cell activation by TLR7 and TLR9 agonists (2008)

These results demonstrate that despite their molecular differences, the TLR7 and TLR9 agonists induce similar genes and proteins in purified human B cells.