gebhart/patrepeval huggingface dataset:
An off-chip antenna for mm-wave applications (2013)

A multi-layer PCB technology incorporating Teflon-based laminates has been used to implement a horn-like antenna for millimeter-wave applications. Although the antenna has a better-than-10-dB return loss for the whole standard 60-GHz band, i. e. 57 GHz - 66 GHz, its radiation pattern changes drastically at the higher-band frequencies. Moreover, due to the very low loss material used for the antenna implementation, the interconnect loss is low when compared to other competitive technologies.
CAT: a high performance, distributed component architecture toolkit for the grid (2003)

Grid systems, such as Globus, Legion and Globe, provide an infrastructure for implementing metacomputing over the Internet. The Component Architecture Toolkit (CAT) provides a software layer above the grid that facilitates programming and end-user interaction with the grid.
Spectroscopic and Microscopic Investigation of Gold Nanoparticle Formation: Ligand and Temperature Effects on Rate and Particle Size (2011)

We report a spectroscopic and microscopic investigation of the synthesis of gold nanoparticles (AuNPs) with average sizes of less than 5 nm. The slow reduction and AuNP formation processes that occur by using 9-borabicyclo[3.3.1]nonane (9-BBN) as a reducing agent enabled a time-dependent investigation based on standard UV-vis spectroscopy and transmission electron microscopy (TEM) analyses. This is in contrast to other borohydride-based syntheses of thiolate monolayer protected AuNPs which form particles very rapidly. We investigated the formation of 1-octadecanethiol (ODT) protected AuNPs with average diameters of 1.5-4.3 nm. By studying the progression of nanoparticle formation over time, we find that the nucleation rate and the growth time, which are interlinked with the amount of ODT and the temperature, influence the size and the size dispersion of the AuNPs. High-resolution TEM (HRTEM) analyses also suggest that the nanoparticles are highly single crystalline throughout the synthesis and appear to be formed by a diffusion-controlled Ostwald-ripening growth mechanism.
The EMED System of foot pressure analysis. (1993)

The EMED System is actually a family of individual modules developed to measure and record pressure distribution under the plantar surface of the foot to provide important information regarding the loading characteristics of the lower extremity. One must distinguish between static measurements taken while the patient is standing and dynamic measurements taken during locomotion. With static measurement, it is not possible to determine the form or the loading pattern of the foot during function. Dynamic measurements are used to determine loading during the actual stance phase of gait, quantified by parameters such as the length and width changes of the foot, angle of gait, digital function, high pressure points, and many other parameters. Important measurement parameters are calculated automatically using either EMED-Intern or EMED-Extern software. Each module of the EMED System is designed specifically to provide the clinician or investigator with the appropriate tool to collect data for analysis of function, direct therapy, and aid in the diagnosis of functional foot problems.
A Fluorescent Interleukin‐8 Receptor Probe Produced by Targetted Labelling at the Amino Terminus (1995)

Interleukin-8 is the most extensively characterised member of the structurally related chemotactic and pro-inflammatory proteins collectively called chemokines. It binds to two closely related members of the seven transmembrane chemokine receptor family found on a variety of leukocyte cell types. In order to study the interaction of interleukin-8 with its receptors, and their distribution, we have produced a fluorescently labelled protein as an alternative to the radioactive 125I-interleukin-8 ligand. Interleukin-8 is naturally produced as two forms, a 72-residue polypeptide by monocytes and a 77-residue form produced by endothelial cells which has an extension of five amino acids at the amino terminal. Both forms are active at nanomolar concentrations, implying that chemical modification to the amino terminus of the 72-residue form will not destroy activity. The 72-residue interleukin-8 sequence starts with a serine residue, which can be oxidised under mild conditions to give a reactive glyoxylyl function which is then reacted with a nucleophilic fluorescein derivative. The site-specifically labelled protein was easily isolated by reverse-phase HPLC. The dissociation constant of the fluorescently labelled interleukin-8 from its receptors on neutrophils was measured by displacement of 125I-interleukin-8 and found to be 10 nM compared to 1 nM for the unmodified protein. The modified protein is highly active in in vitro bioassays using human neutrophils, giving an EC50 of 7 nM in chemotaxis and an EC50 of 0.62 nM for shape change. The binding of the fluorescent protein to neutrophils can also be measured by fluorescent automatic cell sorter (FACS) analysis, and can be competed by unlabelled interleukin-8. The amino-terminal modification of interleukin-8 has produced a reagent which is useful for the quantification of interleukin-8 receptor expression, and will also be useful in monitoring the fate of the ligand after receptor binding.
Search advertising using web relevance feedback (2008)

The business of Web search, a $10 billion industry, relies heavily on sponsored search, whereas a few carefully-selected paid advertisements are displayed alongside algorithmic search results. A key technical challenge in sponsored search is to select ads that are relevant for the user's query. Identifying relevant ads is challenging because queries are usually very short, and because users, consciously or not, choose terms intended to lead to optimal Web search results and not to optimal ads. Furthermore, the ads themselves are short and usually formulated to capture the reader's attention rather than to facilitate query matching.
Protection of NIH 3T3 Cells from Infection by Trypomastigotes and Sphaeromastigotes of Trypanosoma cruzi, Telahuen Strain, by Porphyrins in the Presence and Absence of Light (630 and 690 NM) (1989)

The light and dark toxicities of naturally occurring and synthesized porphyrins were investigated for their potential to protect NIH 3T3 cells from infection with mammalian tissue culture-derived trypomastigotes of Trypanosoma cruzi. The fluorescent nucleic acid dye Hoechst 33342 was employed to trace T. cruzi intracellular development following porphyrin exposure with and without irradiation. The synthetic porphyrins investigated included isomers A and B of mono- and diacid benzoporphyrin (BPD-MA, BPD-DA, BPD-MB, and BPD-DB). derivatives of naturally occurring hematoporphyrin dihematoporphyrin ether (DHE), and hydroxyethylvinyl deuteroporphyrin (HEVD). These porphyrins provided positive protection following irradiation with light Protection also was obtained due to dark toxicity phenomena. HEVD at a concentration of 1.0 micrograms/ml followed by irradiation of 20 J/cm2 of light at 630 nm provided 90% protection, whereas BPD-MA and BPD-MB provided greater than 85% protection using irradiation at 690 nm. Dark protection was greatest at 1.0 micrograms/ml with the BPD (55-70%), but HEVD provided the greatest toxicity (89%) at a concentration of 10 micrograms/ml. Further investigations are in progress to find methods to increase the protection obtained and to determine the mechanism(s) involved in protection.
Photochromic Copolymers Containing Bisthienylethene Units (2004)

Abstract Summary: Two simple yet effective strategies (Wittig condensation reaction and radical polymerization) for the synthesis of photochromic copolymers containing bisthienylethene units are presented. A new family of photochromic copolymer containing bisthienylethene and fluorophores units in the main chains was synthesized by Wittig condensation reaction. Another novel photochromic copolymer with multi‐component photochromic pendent groups was prepared by radical polymerization. Photochromism of the copolymers was investigated in solid film and in solution. Photochromic copolymers containing bisthienylethene units. magnified image Photochromic copolymers containing bisthienylethene units.
Electromechanical Properties of Lead Titanate Zirconate Ceramics Modified with Certain Three‐or Five‐Valent Additions (1959)

When small amounts of lanthana, neodymia, niobia, or tantala are added to lead titanate zirconate compositions near the morphotropic (rhombohedral‐tetragonal) phase boundary, the modified ceramics show piezoelectric planar coupling coefficients of about 0.50 and substantially increased dielectric constants of up to 1545. The mechanical Q factor is decreased to about 70, and there is a marked increase in volume resistivity at temperatures up to 500°C. Other effects noted are better shelf‐aging characteristics, improved stability with respect to temperature dependence of resonant frequency, and a lowering of the Curie temperature. Except with neodymia, a definite refinement of the grain structure is also observed. By similar modification of Pb 0.95 Sr 0.05 (Zr. 0.54 Ti 0.45 )O 3 dielectric constants up to 1792 are obtained along with planar couplings of at least 0.49.
Faire l’inventaire du patrimoine ferroviaire : expériences et méthodes (2009)

Accueillie, passage Colbert, dans les locaux parisiens de l’Institut national du Patrimoine, la journée d’études du 22 octobre 2008 fut organisée conjointement par l’Association pour l’histoire des chemins de fer en France et la mission Inventaire général du patrimoine culturel, qui assure les compétences de l’État dans ce domaine au sein de la sous-direction de l’archéologie, de l’ethnologie, de l’inventaire et du système d’information (direction de l’architecture et du patrimoine, ministère...
Real-time 3D human tracking for mobile robots with multisensors (2017)

Acquiring the accurate 3-D position of a target person around a robot provides fundamental and valuable information that is applicable to a wide range of robotic tasks, including home service, navigation and entertainment. This paper presents a real-time robotic 3-D human tracking system which combines a monocular camera with an ultrasonic sensor by the extended Kalman filter (EKF). The proposed system consists of three sub-modules: monocular camera sensor tracking model, ultrasonic sensor tracking model and multi-sensor fusion. An improved visual tracking algorithm is presented to provide partial location estimation (2-D). The algorithm is designed to overcome severe occlusions, scale variation, target missing and achieve robust re-detection. The scale accuracy is further enhanced by the estimated 3-D information. An ultrasonic sensor array is employed to provide the range information from the target person to the robot and Gaussian Process Regression is used for partial location estimation (2-D). EKF is adopted to sequentially process multiple, heterogeneous measurements arriving in an asynchronous order from the vision sensor and the ultrasonic sensor separately. In the experiments, the proposed tracking system is tested in both simulation platform and actual mobile robot for various indoor and outdoor scenes. The experimental results show the superior performance of the 3-D tracking system in terms of both the accuracy and robustness.
Lentiviral shRNA silencing of murine bone marrow cell CCR2 leads to persistent knockdown of CCR2 function in vivo (2005)

A major barrier in hematopoietic gene function studies is posed by the laborious and time-consuming generation of knockout mice with an appropriate genetic background. Here we present a novel lentivirus-based strategy for the in situ generation of hematopoietic knockdowns. A short hairpin RNA (shRNA) was designed targeting murine CC-chemokine receptor 2 (CCR2), which was able to specifically blunt CCR2 expression at the mRNA, protein, and functional levels in vitro. Reconstitution of irradiated recipient mice with autologous bone marrow that had been ex vivo transduced with shRNA lentivirus led to persistent down-regulation of CCR2 expression, which translated into a 70% reduction in CCR2-dependent recruitment of macrophages to an inflamed peritoneal cavity without noticeable side effects on related chemokine receptors or general inflammation status. These findings clearly demonstrate the potential of shRNA lentivirus-infected bone marrow transplantation as a rapid and effective method to generate hematopoietic knockdowns for leukocyte gene function studies.
Secretion of <i>E</i> , <i>E</i> -Farnesol and Biofilm Formation in Eight Different <i>Candida</i> Species (2008)

Production of E,E-farnesol (FOH) and biofilm formation were studied under various conditions in 56 strains of eight Candida spp. FOH production differed significantly not only between Candida spp. but within Candida albicans strains as well. FOH concentrations and biofilm formation were the highest for C. albicans.
Characterization of the Structure of Carbon Fibers by Wide-Angle and Small-Angle X-ray Scatterings (1989)

A characteristic feature of carbon fiber structure is that the fibers comprise the preferentially oriented carbon-layer stacks and the needle-like microvoids oriented nearly parallel to the fiber axis. In order to study the anisotropic nature of the carbon fiber structure by the X-ray measurement, it is necessary to carry out the measurement on a unidirectionally aligned fiber bundle. For this purpose, it is inappropriate to apply the Bragg, Scherrer, and Warren equations for wide-angle X-ray diffraction and the currently used theories for the small-angle X-ray scattering of isotropic bodies. The present authors have developed previously the methods enabling one to analyse the wide- and small-angle X-ray scatterings from a carbon fiber bundle. In this paper, these methods, together with a convenient method determining the primary beam power by using air scattering, are concisely summerized to facilitate their practical application, and are discussed in relation to the Bragg, Scherrer, and Warren equations. By the proposed methods, the structural parameters characterizing the stacks of carbon layers, such as lattice constants, stacking regularity, sizes, orientation and volume fraction are evaluated from the wide-angle X-ray diffraction intensity distribution, and the characteristic parameters of microvoids, such as cross-sectional sizes, volume fraction, number per unit cross-sectional area of the fiber, inter-void spacing, variation in electron density, crosssectional size distribution and cross-sectional shape are evaluated from the small-angle X-ray scattering intensity distribution.
Fibrin-Targeted PET Probes for the Detection of Thrombi (2013)

There is an ongoing effort to develop better methods for noninvasive detection and characterization of thrombi. Here we describe the synthesis and evaluation of three new fibrin-targeted positron emission tomography (PET) probes (FBP1, FBP2, FBP3). Three fibrin-specific peptides were conjugated as 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)-monoamides at the C- and N-termini and chelated with (64)CuCl2. Probes were prepared with a specific activity ranging from 10 to 130 μCi/nmol. Both the peptides and the probes exhibited nanomolar dissociation constants (Kd) for the soluble fibrin fragment DD(E), although the Cu-DOTA derivatization resulted in a 2-3 fold loss in affinity relative to the parent peptide. Biodistribution and imaging studies were performed in a rat model of carotid artery thrombosis. For FBP1 and FBP2 at 120 min post injection, the vessel containing the thrombus showed the highest concentration of radioactivity after the excretory organs, that is, the liver and kidneys. This was confirmed ex vivo by autoradiography, which showed >4-fold activity in the thrombus-containing artery compared to the contralateral artery. FBP3 showed much lower thrombus uptake, and the difference was traced to greater metabolism of this probe. Hybrid MR-PET imaging with FBP1 or FBP2 confirmed that these probes were effective for the detection of an arterial thrombus in this rat model. A thrombus was visible on PET images as a region of high activity that corresponded to a region of arterial occlusion identified by simultaneous MR angiography. FBP1 and FBP2 represent promising new probes for the molecular imaging of thrombi.
Development of Innovative Pediocin PA-1 by DNA Shuffling among Class IIa Bacteriocins (2007)

Pediocin PA-1 is a member of the class IIa bacteriocins, which show antimicrobial effects against lactic acid bacteria. To develop an improved version of pediocin PA-1, reciprocal chimeras between pediocin PA-1 and enterocin A, another class IIa bacteriocin, were constructed. Chimera EP, which consisted of the C-terminal half of pediocin PA-1 fused to the N-terminal half of enterocin A, showed increased activity against a strain of Leuconostoc lactis isolated from a sour-spoiled dairy product. To develop an even more effective version of this chimera, a DNA-shuffling library was constructed, wherein four specific regions within the N-terminal half of pediocin PA-1 were shuffled with the corresponding sequences from 10 other class IIa bacteriocins. Activity screening indicated that 63 out of 280 shuffled mutants had antimicrobial activity. A colony overlay activity assay showed that one of the mutants (designated B1) produced a >7.8-mm growth inhibition circle on L. lactis, whereas the parent pediocin PA-1 did not produce any circle. Furthermore, the active shuffled mutants showed increased activity against various species of Lactobacillus, Pediococcus, and Carnobacterium. Sequence analysis revealed that the active mutants had novel N-terminal sequences; in active mutant B1, for example, the parental pediocin PA-1 sequence (KYYGNGVTCGKHSC) was changed to TKYYGNGVSCTKSGC. These new and improved DNA-shuffled bacteriocins could prove useful as food additives for inhibiting sour spoilage of dairy products.
Vessel injury and capillary leak (2003)

Our understanding of capillary leak syndrome remains fragmented and weighted toward specific mediators contributing to the leak. The implications of extensive edema and the mechanism by which it resolves continue to be the subject of speculation rather than study.
Tree‐to tree variation on Picea abies in growth inhibition against Fomes annosus (1980)

Abstract The spread of Fomes annosus was measured on a set of 20 mature trees which were artificial inoculated. These observations were compared with growth observations and laccase activity of F. annosus grown on wood dust from the same 20 trees. Although variation between Fomes strains as well as between trees was recorded, correspondance between field and laboratory observations was absent in the present material.
Pain Relief by Electrical Stimulation of the Central Gray Matter in Humans and Its Reversal by Naloxone (1977)

Relief of intractable pain was produced in six human patients by stimulation of electrodes permanently implanted in the periventricular and periaqueductal gray matter. The level of stimulation sufficient to induce pain relief seems not to alter the acute pain threshold. Indiscriminate repetitive stimulation produced tolerance to both stimulation-produced pain relief and the analgesic action of narcotic medication; this process could be reversed by abstinence from stimulation. Stimulation-produced relief of pain was reversed by naloxone in five out of six patients. These results suggest that satisfactory alleviation of persistent pain in humans may be obtained by electronic stimulation.
Steroid Metabolism by Epidermal Keratinocytes<sup>a</sup> (1988)

The metabolism of various radiolabeled steroids by cultured human epidermal keratinocytes was studied in an attempt to identify the steroid-metabolizing enzymes present in these cells. Sulfatase activity was demonstrated in keratinocytes with either E1S or DS as substrates. The products of sulfatase action were E1 and DHEA, respectively. The specific activity of the enzyme was approximately 5- to 14-fold greater with E1S as the substrate compared with DS, and the rates of hydrolysis were linear with incubation time up to 3 h. The metabolism of DHEA by the keratinocyte 17 beta-HSOR-catalyzed reaction resulted in the predominant formation of 5-androstene-3 beta,17 beta-diol. The rate of formation of 5-androstene-3 beta,17 beta-diol was linear with time of incubation up to 18 h, and the specific activity of 17 beta-HSOR, with DHEA as the substrate, was greater in keratinocytes maintained in culture for 4 weeks compared with keratinocytes kept in culture for 1 week. Androstenedione was a minor product of DHEA metabolism. The metabolism of DHT by epidermal keratinocytes resulted in the formation of 5 alpha-androstanedione, 5 alpha-androstane-3 alpha,17 beta-diol, 5 alpha-androstane-3 beta,17 beta-diol, androsterone, and isoandrosterone: the rates of formation of 5 alpha-androstane-3 alpha,17 beta-diol and 5 alpha-androstane-3 beta,17 beta-diol were linear with incubation time up to 24 h, and the specific activities of 3 alpha-HSOR and 3 beta-HSOR did not appear to change with keratinocyte time in culture up to 3 weeks. The metabolism of DOC by epidermal keratinocytes resulted in 5 alpha-dihydrodeoxycorticosterone production: the rate of formation of this metabolite was linear with incubation time up to 4 h. The metabolism of E1 by epidermal keratinocytes yielded E2, and that of E2 resulted in the formation of E1. The rate of E1 formation from E2, was approximately 10-fold greater than the rate of formation of E2 from E1; these rates were linear with incubation time up to 4 h. Epidermal keratinocytes maintained in culture did not metabolize androstenedione to either E1 or E2, and pregnenolone was not metabolized by these cells. This study serves to ascertain that epidermal keratinocytes express steroid 5 alpha-reductase, 17 beta-HSOR, 3 beta-HSOR, 3 alpha-HSOR, 3 beta-hydroxysteroid oxidoreductase-delta 5----4-isomerase, and sulfatase activities.
Kinetic Analysis of Terminal and Unactivated CH Bond Oxyfunctionalization in Fatty Acid Methyl Esters by Monooxygenase‐Based Whole‐Cell Biocatalysis (2011)

Abstract The alkane monooxygenase AlkBGT from Pseudomonas putida GPo1 constitutes a versatile enzyme system for the ω‐oxyfunctionalization of medium chain‐length alkanes. In this study, recombinant Escherichia coli W3110 expressing alkBGT was investigated as whole‐cell catalyst for the regioselective biooxidation of fatty acid methyl esters to terminal alcohols. The ω‐functionalized products are of general economic interest, serving as building blocks for polymer synthesis. The whole‐cell catalysts proved to functionalize fatty acid methyl esters with a medium length alkyl chain specifically at the ω‐position. The highest specific hydroxylation activity of 104 U g CDW −1 was obtained with nonanoic acid methyl ester as substrate using resting cells of E. coli W3110 (pBT10). In an optimized set‐up, maximal 9‐hydroxynonanoic acid methyl ester yields of 95% were achieved. For this specific substrate, apparent whole‐cell kinetic parameters were determined with a V max of 204±9 U g CDW −1 , a substrate uptake constant ( K S ) of 142±17 μM, and a specificity constant V max / K S of 1.4 U g CDW −1 μ M −1 for the formation of the terminal alcohol. The same E. coli strain carrying additional alk genes showed a different substrate selectivity. A comparison of biocatalysis with whole cells and enriched enzyme preparations showed that both substrate availability and enzyme specificity control the efficiency of the whole‐cell bioconversion of the longer and more hydrophobic substrate dodecanoic acid methyl ester. The efficient coupling of redox cofactor oxidation and product formation, as determined in vitro , combined with the high in vivo activities make E. coli W3110 (pBT10) a promising biocatalyst for the preparative synthesis of terminally functionalized fatty acid methyl esters.