gebhart/patrepeval huggingface dataset:
Corticotropin-releasing factor: long-lasting facilitation of the acoustic startle reflex (1992)

Intracerebroventricular infusion of corticotropin-releasing factor (CRF) (0.1-1.0 micrograms) produced a pronounced, dose-dependent enhancement of the acoustic startle reflex in rats. This excitatory effect began about 20-30 min after infusion, grew steadily over the 2 hr test period, and lasted at least 6 hr. Higher doses of CRF (10 micrograms) often produced marked facilitation and then inhibition of startle that oscillated repeatedly with a period of 10-20 min. CRF-enhanced startle did not result from an increase in sensitization produced by repetition of the startle stimulus or from a blockade of habituation. Peripheral injections of the autonomic ganglionic blockers hexamethonium (10 mg/kg) or chlorisondamine (3 mg/kg) slightly attenuated the magnitude of CRF-enhanced startle, suggesting a partial role of peripheral sympathetic activation. Intracerebroventricular infusion of the CRF antagonist alpha-helical CRF9-41 (alpha hCRF; 25 or 50 micrograms) blocked CRF-enhanced startle when infused 5 min prior to CRF, indicating a central site of action. CRF-enhanced startle also was reversed when alpha hCRF was given 90 min after infusion of CRF. This suggests that exogenously applied CRF remains in the brain for a very long time after administration or that CRF given exogenously initiates a process that results in a long-lasting activation of endogenous CRF. Because the startle reflex is elevated by both conditioned and unconditioned fear, these data lend further support to the idea that CRF infusion produces a behavioral state that resembles fear or anxiety. Because startle is mediated by a well-defined neural pathway, CRF-enhanced startle may provide a useful behavioral assay to analyze the neural systems upon which exogenous CRF acts to produce its behavioral effects.
The concentration of the mitochondrial pyruvate carrier in rat liver and heart mitochondria determined with α-cyano-β-(1-phenylindol-3-yl)acrylate (1984)

alpha-Cyano-beta-(1-phenylindol-3-yl)acrylate inhibited pyruvate transport into both liver and heart mitochondria approximately linearly with respect to its concentration until 65% inhibition was achieved. The extent of inhibition was dependent on the mitochondrial protein concentration. By extrapolation of plots of inhibition versus inhibitor concentration to total inhibition, or by mathematical analysis of the plots, the concentration of pyruvate transporter molecules per mg of protein was calculated to be approximately 100 pmol/mg for both heart and liver mitochondria, and the Ki about 7 nM. The data also suggest that pyruvate transport is rate-limiting for pyruvate oxidation by heart mitochondria in State 3, but not by liver mitochondria.
Identification of small molecule compounds targeting the interaction of HIV-1 Vif and human APOBEC3G by virtual screening and biological evaluation (2018)

Human APOBEC3G (hA3G) is a restriction factor that inhibits human immunodeficiency 1 virus (HIV-1) replication. The virally encoded protein Vif binds to hA3G and induces its degradation, thereby counteracting the antiviral activity of hA3G. Vif-mediated hA3G degradation clearly represents a potential target for anti-HIV drug development. Herein, we have performed virtual screening to discover small molecule inhibitors that target the binding interface of the Vif/hA3G complex. Subsequent biochemical studies have led to the identification of a small molecule inhibitor, IMB-301 that binds to hA3G, interrupts the hA3G-Vif interaction and inhibits Vif-mediated degradation of hA3G. As a result, IMB-301 strongly inhibits HIV-1 replication in a hA3G-dependent manner. Our study further demonstrates the feasibility of inhibiting HIV replication by abrogating the Vif-hA3G interaction with small molecules.
Design of a Gag Pentapeptide Analogue that Binds Human Cyclophilin A More Efficiently than the Entire Capsid Protein:  New Insights for the Development of Novel Anti-HIV-1 Drugs (2000)

Cyclophilin A (hCyp-18), a ubiquitous cytoplasmic peptidyl-prolyl cis/trans isomerase (PPIase), orchestrates HIV-1 core packaging. hCyp-18, incorporated into the virion, enables core uncoating and RNA release and consequently plays a critical role in the viral replication process. hCyp-18 specifically interacts with a single exposed loop of the Gag polyprotein capsid domain via a network of nine hydrogen bonds which mainly implicates a 7-mer fragment of the loop. As previously reported, the corresponding linear heptapeptide Ac-Val-His-Ala-Gly-Pro-Ile-Ala-NH(2) (2) binds to hCyp-18 with a low affinity (IC(50) = 850 +/- 220 microM) but a potentially useful selectivity for hCyp-18 relative to hFKBP-12, another abundant PPIase. On the basis of X-ray structures of Gag fragments:hCyp-18 complexes, we generated a series of modified peptides in order to probe the determinants of the interaction and hence to select a peptidic ligand displaying a higher affinity than the capsid domain of Gag. We synthesized a series of heptapeptides to test the energetic contribution of amino acids besides the Gly-Pro moiety. In particular the importance of the histidine residue for the interaction was underscored. We also investigated the influence of N- and C-terminal modifications. Hexapeptides containing either deaminovaline (Dav) in place of the N-terminal valine or substitution of the C-terminal alanine amide with a benzylamide group displayed increased affinities. Combination of both modifications gave the most potent competitor Dav-His-Ala-Gly-Pro-Ile-NHBn (28) which has a higher affinity for hCyp-18 (K(d) = 3 +/- 0.5 microM) than the entire capsid protein (K(d) = 16 +/- 4 microM) and a very low affinity for hFKBP-12. Some of our results strongly suggest that the title compound is not a substrate of hCyp-18 and interacts preferentially in the trans conformation.
Electrical stimuli improve osteogenic differentiation mediated by aniline pentamer and PLGA nanocomposites (2013)

Electrical stimulation may improve the proliferation of animal cells. In the present study, osteoblasts were cultured on electroactive aniline pentamer (AP)/poly(lactic-co-glycolic acid) (PLGA) copolymer composites, on which electric pulse was imposed. The combination of polymer and electric pulse enhanced the osteogenic differentiation of the osteoblasts, characterized by the upregulated expression of bone morphogenetic protein (BMP)-2, collagen I and osteonectin and the phosphorylation of Samd4, in contrast to polymer or electrical pulse alone. This action occurred in a polymer content-dependent manner. Therefore, the action of the electric pulse, assisted by the electroactive polymer implant, may be promising in the expedition of injured bone repair.
An efficient certificate revocation validation scheme with k-means clustering for vehicular ad hoc networks (2012)

Vehicular ad hoc networks (VANETs) are emerging as a promising approach to improve traffic safety and provide a wide range of wireless applications to passengers in vehicles. To launch reliable and trusted vehicular communications, one prerequisite is to ensure peer vehicles' credibility, by means of digital certificate validation from messages that are sent out by other vehicles. However, in vehicular communication systems, certificate validation is more time sensitive than in traditional networks, because each vehicle receives a large number of messages in a short period of time. Another issue that needs to be tackled is the unsuccessful delivery of information between vehicles and other entities on the road due to their high rate of mobility. For these reasons, we need new solutions to speed up the process of certificate validation. In this article, we propose a certificate revocation status validation scheme; this uses the concept of clustering in the realm of data mining, which can meet the aforementioned requirements. We employ the technique of k-means clustering to boost up the efficiency of certificate validation, thereby enhancing the security of VANETs. Additionally, a comprehensive analysis in terms of security for the scheme is presented; and the analytical results demonstrate that this scheme can effectively improve the validation of a certificate, thus securing the vehicular communication in vehicular networks.
Phenotypic Reversion or Death of Cancer Cells by Altering Signaling Pathways in Three-Dimensional Contexts (2002)

The 3D rBM assay can be used to identify signaling pathways that, when manipulated in concert, can lead to the restoration of morphologically normal breast structures or to death of the tumor cells, even highly metastatic cells. This approach may be useful to design therapeutic intervention strategies for aggressive breast cancers.
Intercomparison of Daegwallyeong Cloud Physics Observation System (CPOS) Products and the Visibility Calculation by the FSSP Size Distribution during 2006-2008 (2010)

To observe and analyze the characteristics of cloud and precipitation properties, the Cloud physics Observation System (CPOS) has been operated from December 2003 at Daegwallyeong (, , 842 m) in the Taebaek Mountains. The major instruments of CPOS are follows: Forward Scattering Spectrometer Probe (FSSP), Optical Particle Counter (OPC), Visibility Sensor (VS), PARSIVEL disdrometer, Microwave Radiometer (MWR), and Micro Rain Radar (MRR). The former four instruments (FSSP, OPC, visibility sensor, and PARSIVEL) are for the observation and analysis of characteristics of the ground cloud (fog) and precipitation, and the others are for the vertical cloud characteristics (http://weamod.metri.re.kr) in real time. For verification of CPOS products, the comparison between the instrumental products has been conducted: the qualitative size distributions of FSSP and OPC during the hygroscopic seeding experiments, the precipitable water vapors of MWR and radiosonde, and the rainfall rates of the PARSIVEL(or MRR) and rain gauge. Most of comparisons show a good agreement with the correlation coefficient more than 0.7. These reliable CPOS products will be useful for the cloud-related studies such as the cloud-aerosol indirect effect or cloud seeding. The visibility value is derived from the droplet size distribution of FSSP. The derived FSSP visibility shows the constant overestimation by 1.7 to 1.9 times compared with the values of two visibility sensors (SVS (Sentry Visibility Sensor) and PWD22 (Present Weather Detect 22)). We believe this bias is come from the limitation of the droplet size range () measured by FSSP. Further studies are needed after introducing new instruments with other ranges.
4-Aminoquinolone Piperidine Amides: Noncovalent Inhibitors of DprE1 with Long Residence Time and Potent Antimycobacterial Activity (2014)

4-Aminoquinolone piperidine amides (AQs) were identified as a novel scaffold starting from a whole cell screen, with potent cidality on Mycobacterium tuberculosis (Mtb). Evaluation of the minimum inhibitory concentrations, followed by whole genome sequencing of mutants raised against AQs, identified decaprenylphosphoryl-β-d-ribose 2'-epimerase (DprE1) as the primary target responsible for the antitubercular activity. Mass spectrometry and enzyme kinetic studies indicated that AQs are noncovalent, reversible inhibitors of DprE1 with slow on rates and long residence times of ∼100 min on the enzyme. In general, AQs have excellent leadlike properties and good in vitro secondary pharmacology profile. Although the scaffold started off as a single active compound with moderate potency from the whole cell screen, structure-activity relationship optimization of the scaffold led to compounds with potent DprE1 inhibition (IC50 < 10 nM) along with potent cellular activity (MIC = 60 nM) against Mtb.
Epidermal Patch with Glucose Biosensor: pH and Temperature Correction toward More Accurate Sweat Analysis during Sport Practice (2020)

We present an epidermal patch for glucose analysis in sweat incorporating for the first time pH and temperature correction according to local dynamic fluctuations in sweat during on-body tests. This sort of correction is indeed the main novelty of the paper, being crucial toward reliable measurements in every sensor based on an enzymatic element whose activity strongly depends on pH and temperature. The results herein reported for corrected glucose detection during on-body measurements are supported by a two-step validation protocol: with the biosensor operating off- and on-bodily, correlating the results with UV-vis spectrometry and/or ion chromatography. Importantly, the wearable device is a flexible skin patch that comprises a microfluidic cell designed with a sweat collection zone coupled to a fluidic channel in where the needed electrodes are placed: glucose biosensor, pH potentiometric electrode and a temperature sensor. The glucose biosensor presents a linear range of response within the expected physiological levels of glucose in sweat (10-200 μM), and the calibration parameters are dynamically adjusted to any change in pH and temperature during the sport practice by means of a new "correction approach". In addition, the sensor displays a fast response time, appropriate selectivity, and excellent reversibility. A total of 9 validated on-body tests are presented: the outcomes revealed a great potential of the wearable glucose sensor toward the provision of reliable physiological data linked to individuals during sport activity. In particular, the developed "correction approach" is expected to impact into the next generation of wearable devices that digitalize physiological activities through chemical information in a trustable manner for both sport and healthcare applications.
Robust channel estimation and ISI cancellation for OFDM systems with suppressed features (2005)

A feature-suppressed orthogonal frequency-division multiplexing (OFDM) system and the corresponding channel estimation and intersymbol interference (ISI) mitigation techniques are investigated in this paper. Cyclic prefix (CP) and pilot tones, which are commonly used in civilian OFDM systems for ISI mitigation and channel estimation, create distinctive waveform features that can be easily used for synchronization and channel estimation purposes by intercepting receivers. As a result, CP and pilot tones are eliminated in the proposed feature suppressed OFDM system to reduce the interception probability. Instead, a set of specially designed OFDM symbols, driven by different pseudorandom sequences, are employed as preambles to avoid unique spectral signature. These preambles are inserted into the OFDM data symbol stream periodically and in a round-robin manner. In addition, a random frequency offset is introduced to each preamble to further mask the multicarrier signature. New challenges arising from these feature suppression efforts are studied, including robust channel estimation and demodulation techniques in the presence of frequency offset and severe interference. Based on our interference analysis, an iterative ISI and intercarrier interference (ICI) estimation-cancellation-based technique is proposed for both channel estimation and OFDM data demodulation. Our channel estimator performs joint frequency offset and channel impulse response estimation based on the maximum-likelihood (ML) principle. To reduce its complexity, we employ a number of techniques, which include approximation of the ML metrics, as well as fast Fourier transform pruning. The performances and feasibility of the proposed feature suppressed OFDM system and the channel estimator are analyzed and verified through numerical simulations.
Preventive Effect of D-002, a Mixture of Long-Chain Alcohols from Beeswax, on the Liver Damage Induced with CCl <sub>4</sub> in Rats (2007)

D-002 is a mixture of higher aliphatic primary alcohols purified from beeswax with antioxidant effects. Acute hepatotoxicity induced with CCl4 in rats has been related to increased hepatic lipid peroxidation and prevented with some antioxidants. This study investigated whether D-002 could prevent the acute CCl4-induced hepatotoxicity in rats. Animals were randomly distributed into four groups: a negative control treated orally with the vehicle and three groups injected with CCl4 (1 mL/kg) and treated orally either with the vehicle (positive control) or with D-002 (25 and 100 mg/kg). Eighteen hours after CCl4 dosing, rats were anesthetized, and livers were removed for histopathological studies. Some portions were taken and homogenized for assessing malondialdehyde (MDA) concentrations. Positive, but not negative, controls showed ballooned cells, swollen hepatocytes, and lipid-included hepatocytes, as well as necrotic areas and inflammatory infiltrates. D-002 (25 and 100 mg/kg) dose-dependently and significantly (P < .01) decreased the frequency of all abnormal liver cell types and increased that of normal hepatocytes compared with the positive controls, not showing necrotic areas or inflammatory infiltrates. D-002 dose-dependently decreased hepatic MDA levels, but only in the highest dose group were these levels significantly lower than in the positive control. In conclusion, D-002 effectively prevented the histological liver disturbances and lowered MDA levels, a marker of cellular lipid peroxidation, in rats treated with CCl4. Since increased liver lipid peroxidation has been postulated as a cause of CCl4-induced liver damage in rats, the preventive effects of D-002 could be due to its antioxidant action, but such a proposal still requires further research.
Neurogenesis in Adult Subventricular Zone (2002)

Much excitement has been generated by the identification of adult brain regions harboring neural stem cells and their continual generation of new neurons throughout life. This is an important departure from traditional views of the germinal potential of the postnatal brain. However, a more profound
Proceedings of the International Working Conference on Advanced Visual Interfaces (2012)

Since 1992, the International Working Conference on Advanced Visual Interfaces (AVI) has become a systematic biennal appointment for a wide International community of highly qualified researchers, who share the interest in the conception, design and implementation of innovative interfaces promoting individual and societal life quality improvements.

The 2012 edition has taken place in the spectacular scenario of Capri island (Italy), and has attracted an outstanding number of research paper submissions from 31 countries from 5 continents, which proves the ever growing interest that the covered research areas are attracting from the International scientific community. We were honored to count on the profound expertise of the members of the Program Committee to carry out a rigorous peer-reviewing process, which allowed us to select 54 high quality full papers, out of 193 submissions, with an acceptance rate of 28%. Moreover, we were able to select 46 short papers, out of 101 submissions describing promising work in progress, deserved to be presented to stimulate brainstorming discussions among participants on the envisaged research challenges. As a result, the technical program covers several interesting topics related to perceptual interface design, implementation, productization, evaluation and deployment.
Growth of AlN/GaN layered structures by gas source molecular-beam epitaxy (1990)

AlN/GaN layered structures with layer periods between 1.5 and 40 nm have been grown on (0001) oriented sapphire and α(6H)-SiC substrates. The growth was performed using a modified gas source molecular-beam epitaxy (MBE) technique. Standard effusion cells were used as sources of Al and Ga, and a small, MBE compatible, electron cyclotron resonance (ECR) plasma source was used to activate nitrogen gas prior to deposition. Chemical analysis of the layers was conducted using Auger spectrometry. X-ray diffractometry, transmission electron microscopy (TEM), and high-resolution electron microscopy (HREM) were employed for the structural and microstructural studies. Coherent interfaces (no relaxation by misfit dislocations) were observed for bilayer periods smaller than 6 nm. By contrast, completely relaxed individual layers of GaN and AlN with respect to each other were present for bilayer periods above 20 nm. Cathodoluminescence showed a shift in the emission peak from 3.42 eV for the sample with individual 10-nm thick GaN wells and AlN barriers to 4.11 eV for the sample having 1-nm thick individual layers.
Single sided thermal diffusivity imaging with a shuttered thermographic inspection system (2002)

A measurement configuration is discussed where shutters are placed in front of the imager and the flash lamps. Proper timing for the opening and closing of the shutters eliminates a direct interaction between the imager and flash lamps for a single sided measurement, enabling accurate measurement of the thermal response of a specimen. The time dependence of the thermal response is analyzed to produce an image of the effective diffusivity of the specimen.
Neuroblastoma in adults and adolescents (1997)

Neuroblastoma in adolescents and adults has different biologic characteristics and a longer course than in children; nevertheless, ultimately the outcome is poor regardless of stage. A much more aggressive or innovative therapeutic approach is needed for these patients.
2010 report from the Center for International Blood and Marrow Transplant Research (CIBMTR): current uses and outcomes of hematopoietic cell transplants for blood and bone marrow disorders. (2010)

These data indicate increasing use of HCT for persons with blood and bone marrow disorders. Recent trends include increasing use of alternative donors including HLA-matched unrelated persons and of HLA-matched umbilical cord blood cells, increasing use of blood cell rather than bone marrow grafts and increasing use of reduced-intensity pretransplant conditioning regimens. Many of these shifts are driven by logistical considerations like the need for donors in persons without an HLA-identical sibling or expanding access to allotransplants to older patients. In other instances, like the shift from bone marrow to blood cell grafts or from conventional to reduced-intensity pretransplant conditioning regimens few randomized clinical trials have been reported to justify these shifts. More data are needed to critically-assess the impact of these changes.
Transforming Growth Factor βl-Responsive Element: Closely Associated Binding Sites for USF and CCAAT-Binding Transcription Factor-Nuclear Factor I in the Type 1 Plasminogen Activator Inhibitor Gene (1992)

Transforming growth factor beta (TGF-beta) is the name of a group of closely related polypeptides characterized by a multiplicity of effects, including regulation of extracellular proteolysis and turnover of the extracellular matrix. Its cellular mechanism of action is largely unknown. TGF-beta 1 is a strong and fast inducer of type 1 plasminogen activator inhibitor gene transcription. We have identified a TGF-beta 1-responsive element in the 5'-flanking region of the human type 1 plasminogen activator inhibitor gene and shown that it is functional both in its natural context and when fused to a heterologous nonresponsive promoter. Footprinting and gel retardation experiments showed that two different nuclear factors, present in extracts from both TGF-beta 1-treated and nontreated cells, bind to adjacent sequences contained in the responsive unit. A palindromic sequence binds a trans-acting factor(s) of the CCAAT-binding transcription factor-nuclear factor I family. A partially overlapping dyad symmetry interacts with a second protein that much evidence indicates to be USF. USF is a transactivator belonging to the basic helix-loop-helix family of transcription factors. Mutations which abolish the binding of either CCAAT-binding transcription factor-nuclear factor I or USF result in reduction of transcriptional activation upon exposure to TGF-beta 1, thus showing that both elements of the unit are necessary for the TGF-beta 1 response. We discuss the possible relationship of these findings to the complexity of the TGF-beta action.