gebhart/patrepeval huggingface dataset:
ERG K <sup>+</sup> channel blockade enhances firing and epinephrine secretion in rat chromaffin cells: the missing link to LQT2‐related sudden death? (2002)

The ether-a-go-go-related genes (erg) are expressed in tissues other than heart and brain, in which human erg (HERG) K+ channels are known to regulate the repolarization of heart action potentials and neuronal spike-frequency accommodation. We provide evidence that erg1 transcripts and ERG proteins are present in rat chromaffin cells in which we could isolate a K+ current that was biophysically and pharmacologically similar to the ERG current. Firing frequency and catecholamine release were analyzed at the single-cell level by means of perforated patch-clamp and carbon fiber electrochemical detection. It was found that the blocking of ERG, KATP, and KCa channels led to hyperexcitability and an increase in catecholamine release. Combined immunocytochemical experiments with antibodies directed against phenylethanolamine N-methyltransferase and ERG channels suggested expression of these channels in epinephrine- but not in norepinephrine-containing cells. It is concluded that, in addition to being crucial in regulating the QT period in the heart, ERG channels play a role in modulating epinephrine, a fundamental neurotransmitter shaping cardiac function. This finding suggests that the sudden death phenotype associated with LQT2 syndrome mutations may be the result of an emotionally triggered increase in epinephrine in a long-QT running heart.
The Anti-Addiction Drug Ibogaine and the Heart: A Delicate Relation (2015)

The plant indole alkaloid ibogaine has shown promising anti-addictive properties in animal studies. Ibogaine is also anti-addictive in humans as the drug alleviates drug craving and impedes relapse of drug use. Although not licensed as therapeutic drug and despite safety concerns, ibogaine is currently used as an anti-addiction medication in alternative medicine in dozens of clinics worldwide. In recent years, alarming reports of life-threatening complications and sudden death cases, temporally associated with the administration of ibogaine, have been accumulating. These adverse reactions were hypothesised to be associated with ibogaine's propensity to induce cardiac arrhythmias. The aim of this review is to recapitulate the current knowledge about ibogaine's effects on the heart and the cardiovascular system, and to assess the cardiac risks associated with the use of this drug in anti- addiction therapy. The actions of 18-methoxycoronaridine (18-MC), a less toxic ibogaine congener with anti-addictive properties, are also considered.
Adhesion of uric acid crystals to the surface of renal epithelial cells (2000)

Adhesion of microcrystals that nucleate in tubular fluid to the apical surface of renal tubular cells could be a critical step in the formation of kidney stones, 12% of which contain uric acid (UA) either alone or admixed with calcium oxalates or calcium phosphates. UA crystals bind rapidly to monolayer cultures of monkey kidney epithelial cells (BSC-1 line), used to model the surface of the nephron, in a concentration-dependent manner. The urinary glycoproteins osteopontin, nephrocalcin, and Tamm-Horsfall glycoprotein had no effect on binding of UA crystals to the cell surface, whereas other polyanions including specific glycosaminoglycans blocked UA crystal adhesion. Specific polycations also inhibited adhesion of UA crystals and appeared to exert their inhibitory effect by coating cells. However, removal of anionic cell surface molecules with neuraminidase, heparitinase I, or chondroitinase ABC each increased UA crystal binding, and sialic acid-binding lectins had no effect. These observations suggest that hydrogen bonding and hydrophobic interactions play a major role in adhesion of electrostatically neutral UA crystals to renal cells, unlike the interaction of calcium-containing crystals with negatively charged molecules on the apical cell surface via ionic forces. After adhesion to the plasma membrane, subsequent cellular events could contribute to UA crystal retention in the kidney and the development of UA or mixed calcium and UA calculi.
Synthesis of High-Temperature Titania−Alumina Supports (2006)

Catalyst support materials based upon composite metal oxides often incorporate the beneficial aspects of the constituents. For the case of TiO2−Al2O3 composites, however, it has proven difficult to retain a high anatase content and surface area at temperatures of 900 °C and above. This work reports a sol−gel synthesis method that solves these problems. The mixed oxide incorporates 100% anatase regardless of composition and at a Ti:Al-0.4:0.6 weight ratio has a surface area 74 m2/g. Both properties are stable after annealing up to total times of 34 h at 900 °C. Experiments with supported Pd show that metal dispersion on the mixed oxide is observed roughly twice that of a pure Al2O3 support. The action of acetic acid in the preparation mixture during sol formation and calcination, augmented by solid solution formation between TiO2 and Al2O3 during calcinations, seems to account for these results.
Continuously tunable optical filter for use in resonance Raman spectroscopy (1977)

A tunable optical filter is described for removing undesired emissions from laser beams for use in resonance Raman and fluorescence spectroscopy. The bandpass is 1 nm, and the filter is tunable from 400 nm to 750 nm. The degree of polarization of the dye laser beam is improved by the filter. Two independent controls tune the filter to the selected frequency, while the input and output beam directions remain fixed.
Performance of a simple chromatin-rich segmentation algorithm in quantifying basal cell carcinoma from histology images (2012)

The proposed image analysis algorithm demonstrates the feasibility of automatically extracting tumour regions from digitized basal cell carcinoma histology slides. The proposed algorithm may be adaptable to other stain combinations and tumour types.
Engineering Heritage: Beyond the Technical (2003)

The concept of engineering heritage encompasses more than recording and preserving evidence of past technical achievements. An overemphasis on technical criteria can result in the virtual exclusion of equally important social, political and economic determinants. The recognition of engineering as a profession owed much to the public perception that its members worked rationally and objectively in the public interest, while engineers themselves valued their belief in technical knowledge and skills as a means of resolving social problems. Moreover, the multi-faceted nature of engineering heritage is readily accommodated in Queensland's heritage legislation provisions. Of the eight criteria used to determine the cultural significance of a 'place', four are concerned with social, cultural and historical identifiers. In this paper, by examining the social, political and economic, as well as the technical, aspects of two Queensland public works projects, I wish to present a view of engineering heritage as the sum of many parts.
Isolation and characterization of human <i>reg</i> protein produced in <i>Saccharomyces cerevisiae</i> (1990)

reg was originally identified as a gene expressed during the regeneration of insulin-producing pancreatic beta-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino-terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of alpha-helix and beta-sheet contents.
Un estudio sobre la relación entre las notas de Matemáticas Empresariales y Estadística Básica para las Ciencias Sociales en la ULPGC (2014)

En este trabajo se estudia la relación entre las notas obtenidas, en las asignaturas Ma-temáticas Empresariales y Estadística Básica para las Ciencias Sociales, por los estudiantes de primero del Grado de Administración y Dirección de Empresas de la Universidad de Las Palmas de Gran Canaria durante el curso académico 2012-2013. Para ello se considera un modelo estadístico basado en el uso de una distribución discreta bivariante como es la distribución Poisson bivariante, que permite, una vez estudiada la validación del modelo a la muestra disponible, analizar el comportamiento de la nota media de la asignatura de Estadística Básica para las Ciencias Sociales (impartida en el segundo semestre) dadas las notas de la asignatura Matemáticas Empresariales (impartida en el primer semestre), obteniendo así un modelo con carácter predictivo. Los resultados muestran una fuerte dependencia de las notas logradas por un estudiante en la asignatura de Estadística Básica para las Ciencias Sociales condicionada a las notas alcanzadas en Matemáticas Empresa-riales, observándose por tanto un rendimiento académico similar en ambas asignaturas.
<i>Agrobacterium tumefaciens</i>‐mediated barley transformation (1997)

Genetically transformed barley was produced by eco‐cultivating immature embryo explants with Agrobacterium tumefaciens carrying a binary vector coding for chimaeric bacterial genes, bar and gus , and selecting for bialaphos‐resistant cultures from which plants were regenerated. Integration of both genes was confirmed by gel blot hybridization analysis of DNA from the transformed plants and their progenies. From 1282 embryos, plants were recovered for 54 independently transformed lines, giving a transformation efficiency of 4.2%. Transgene numbers in the different lines ranged from single copy insertion to at least ten copies. Sixteen out of 18 plants grown to maturity were fully fertile. Both marker genes, bar and gus , were expressed and co‐segregated in the T 1 progeny plants. In the majority of cases, the genes showed Mendelian segregation predicted for transgene insertion at a single locus. In one family with multiple transgene insertions, molecular analysis of T 1 and T 2 plants suggested that the T‐DNA had inserted at two unlinked loci.
Thermal stresses in aluminum lines bounded to substrates (1992)

Thermal stresses in unpassivated and passivated aluminum thin-film lines bonded to substrates have been calculated using finite-element techniques. For the unpassivated lines, only elastic behavior was considered. The stresses were found to increase with increasing linewidth and with increasing substrate rigidity. Edge effects were found to become nonnegligible for narrow lines. For passivated lines, allowed to deform either elastically or in elastic perfectly plastic manner, the stresses increase with decreasing linewidth, with increasing passivation thickness, and with increasing passivation stiffness. Stresses in elastic perfectly plastic lines are slightly lower and have flatter profiles than stresses in purely elastic lines.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Endostatin-Cytosine Deaminase Fusion Protein Suppresses Tumor Growth by Targeting Neovascular Endothelial Cells (2006)

Endostatin, an angiogenesis inhibitor tested in multiple clinical trials, selectively targets neovascular endothelial cells, suppressing tumor growth. To enhance the therapeutic efficacy of endostatin, we fused endostatin with cytosine deaminase, which converts a prodrug 5-flucytosine into a cytotoxic 5-fluorouracil. This therapeutic strategy was developed based on the observation that the endostatin-green fluorescence protein gene and endostatin-luciferase gene selectively target to endothelial cells in vitro and to the tumor site in vivo, respectively. When we used the endostatin-cytosine deaminase fusion protein to treat s.c. grafted tumors or experimental metastasis tumors, our results showed that endostatin-cytosine deaminase treatment provided stronger tumor growth suppression and increased mean survival time of the mice compared with the treatments of endostatin alone, cytosine deaminase alone, or endostatin plus cytosine deaminase. The endostatin-cytosine deaminase protein significantly inhibited the growth of endothelial cells and preferentially induced tumor cell apoptosis. This endostatin-cytosine deaminase fusion approach opens an avenue for cancer-targeting therapy.
Methods for quantitation of gene expression (2009)

Gene expression of protein encoding genes can be quantitatively measured at the transcriptional level by a number of low- to high-throughput methods. The sensitivity of each method is dependent on both the intrinsic properties of the respective technology and the absolute number of each mRNA molecule to be measured. For these reasons, the correlation of measurements between technological platforms may be variable. Due to the complexity of the transcriptome, the purpose of a gene expression study dictates the choice of method as each is connected to a set of advantages and disadvantages. Strategies such as global mRNA amplification of small samples, have been implemented to overcome previous limitations. However, stochastic events will limit quantitative measurements of any tool when in-put levels are extremely low. Due to the versatile nature of microarray technology, this method will likely persist as a highly applied tool to query the levels of non-coding transcripts, a new expansion in the field of gene expression analysis although possible advances of the technology may occur.
<i>Short-Term Preservation of Mouse Bone Marrow at Refrigeration and Room Temperature for Irradiation Experiments</i> (1957)

Storage of normal mouse bone marrow was carried out to determine the maximum time donor marrow cells were capable of promoting recovery in lethally irradiated mice. The mouse bone marrow was stored in cell suspension, in intact excised femurs, and in intact femurs in the mouse cadaver. Temperature conditions of storage were refrigeration (2°–5°C) and room temperature (22°–25°C). Tyrode's solution was employed as the storage medium. Evaluation of the stored marrow was based on its ability to protect isologous lethally irradiated mice. Maximum storage time at 2°–5°C for cadaver femurs was 3 days, for excised femurs it was 4 days and for cell suspensions it was 5 days. Donor marrow stored at room temperature was effective in cell suspensions up to 2 days and in intact femurs for 1 day. Cadaver femurs showed no activity after 1 day. Short-term preservation of mouse bone marrow for irradiation experiments is feasible for a few days, depending on the conditions of storage and the temperature of storage. Submitted on October 11, 1956
A theoretical model of film condensation in a bundle of horizontal low finned tubes. (1988)

A previously proposed theoretical model of film condensation on a single horizontal low finned tube is extended to the case of a horizontal tube bundle with negligible vapor shear. Based on the flow characteristics of condensate on a vertical column of horizontal low finned tubes, two major flow modes, the column mode and the sheet mode, are considered. It is shown that the present model can predict the heat transfer coefficient satisfactorily for available experimental data including four fluids and five tube columns. Sample calculations show that the effects of the fin spacing and the number of vertical tube rows on the heat transfer performance are significant for R-12 with small values of σ and Δh, while these effects are small for steam with large σ and Δh values.
13‐Cis retinoic acid can enhance the antitumor activity of non‐replicating <scp>S</scp>endai virus particle against neuroblastoma (2012)

Hemagglutinating virus of Japan-envelope (HVJ-E) is a drug delivery vector based on inactivated Sendai virus. Recently, antitumor activities were found for HVJ-E itself and clinical trials of HVJ-E for some malignant tumors are now ongoing. We investigated the in vitro and in vivo antitumor effects of HVJ-E against neuroblastoma, which is one of the most common malignant solid tumors in childhood. The sensitivity of human neuroblastoma cell lines to HVJ-E correlated with the expression level of gangliosides, Sialylparagloboside (SPG) and GD1a, receptors for HVJ. Among the cell lines, SK-N-SH was the most sensitive to HVJ-E in vitro and total SPG and GD1a expression was the highest. Complete eradication of subcutaneous tumors derived from SK-N-SH cells was achieved by intratumoral injection of HVJ-E in SCID mice and no recurrence was observed for more than 300 days after HVJ-E inoculation. In contrast, NB1 cells expressed the lowest amount of GD1a and SPG and were resistant to HVJ-E in vitro. The expression of GD1a increased by 13-cis retinoic acid (13cRA), which is a therapeutic drug for high risk neuroblastoma, thus leading to an improved sensitivity to HVJ-E in vitro. Only growth inhibition of the subcutaneous tumors derived from NB1 cells was achieved by HVJ-E in the SCID mice, but the combination of 13cRA and HVJ-E could achieve partial eradication of the xenograft and also lead to an improved prognosis. In conclusion, HVJ-E is a promising therapeutic modality for neuroblastoma and 13cRA can be used as an adjuvant to HVJ-E.
Computed tomography with microwaves. (1980)

Computed tomographic images were generated using collimated microwaves of frequency 10.5 GHz instead of x rays. In the authors' laboratory version of a rotate-translate scanner, the beam is fixed and a phantom is moved between source and detector, the apparatus being suspended in a tank of fluid to provide impedance matching between phantom, source, and receiver. Phantoms consisting of test tubes of water, cylinders of wood, and animal tissues show spatial resolutions of about 2 cm. It is planned to apply the technique first to mammographic examination, which can best utilize the large difference in microwave absorption between fatty and nonfatty tissues.
Ion Concentration Polarization by Bifurcated Current Path (2017)

Abstract Ion concentration polarization (ICP) is a fundamental electrokinetic process that occurs near a perm-selective membrane under dc bias. Overall process highly depends on the current transportation mechanisms such as electro-convection, surface conduction and diffusioosmosis and the fundamental characteristics can be significantly altered by external parameters, once the permselectivity was fixed. In this work, a new ICP device with a bifurcated current path as for the enhancement of the surface conduction was fabricated using a polymeric nanoporous material. It was protruded to the middle of a microchannel, while the material was exactly aligned at the interface between two microchannels in a conventional ICP device. Rigorous experiments revealed out that the propagation of ICP layer was initiated from the different locations of the protruded membrane according to the dominant current path which was determined by a bulk electrolyte concentration. Since the enhancement of surface conduction maintained the stability of ICP process, a strong electrokinetic flow associated with the amplified electric field inside ICP layer was significantly suppressed over the protruded membrane even at condensed limit. As a practical example of utilizing the protruded device, we successfully demonstrated a non-destructive micro/nanofluidic preconcentrator of fragile cellular species ( i . e . red blood cells).
Semi-Retractable Current Leads for a 21 T FT-ICR Magnet System (2008)

The development of the semi-retractable current leads for a 21 T Fourier transform ion cyclotron resonance (FT-ICR) superconducting magnet system is presented. The semi-retractable current leads are composed of a normal metal element, conducting the current from room temperature to intermediate temperature, and an HTS element, conducting the current down to liquid helium temperature. An HTS element is partly immersed in liquid helium and the joint between the normal metal and HTS element is continuously refrigerated by a cryocooler. After magnet energization the metal element is disengaged from the HTS element without breaking vacuum to the insulating vacuum space. In the paper, the optimized dimensions of the leads are presented in order to minimize the thermal heat load when carrying operational current with some margin. The intermediate block with a lockable set point and the insulating vacuum system are fabricated and the adaptability and reliability are tested during engage and disengage performance. The effects of vacuum level and performance cycle on the electrical contact resistance in a lockable set are also investigated.
Flight Qualified Pulsed Electric Thruster for Satellite Control (1974)

Covers advancements in spacecraft and tactical and strategic missile systems, including subsystem design and application, mission design and analysis, materials and structures, developments in space sciences, space processing and manufacturing, space operations, and applications of space technologies to other fields.
Large-scale DNA microarray analysis of atopic skin lesions shows overexpression of an epidermal differentiation gene cluster in the alternative pathway and lack of protective gene expression in the cornified envelope (2005)

Our results, showing downregulation of the cornified envelope genes and upregulation of the alternative keratinization pathway, are the first to suggest abnormal epidermal differentiation and defective defences as key abnormalities in AD.
An implementation of BCH codes in a FPGA (2010)

Our paper presents the prototyping of a BCH (Bose, Chaudhuri, and Hocquenghem) encoder and decoder using a Field Programmable Gate Array (FPGA) reconfigurable chip. These types of codes are used in communications networks to detect and correct errors. Typically, the algorithms that implement these codes are sequentially type. Our solution is a combination of a parallel and a sequential implementation. We implemented the BCH code in a 3s400FG456 FPGA. In this implementation we used 15 bits-size word code and the results show that the circuits work quite well.
Epitaxial NiInGaAs formed by solid state reaction on In0.53Ga0.47As: Structural and chemical study (2013)

Thin epitaxial layers of NiInGaAs formed by solid state reaction of Ni on (100) In0.53Ga0.47As are used as metal source and drain regions for In0.53Ga0.47As metal oxide field effect transistors. Here, the authors present a structural and chemical analysis of this phase. The stoichiometry of the layer was determined as Ni2In0.53Ga0.47As. Transmission electron microscopy revealed an abrupt interface and a detailed x-ray diffraction analysis showed that the layer is of a hexagonal lattice, which grows epitaxially with the orientation relations of {100}InGaAs||{100}NiInGaAs; ⟨011¯⟩InGaAs||[001]NiInGaAs. Only one domain can be observed in this epitaxial growth. Understanding the structure of these layers is a crucial step not only in their incorporation into InGaAs based devices but also a step toward novel devices.