gebhart/patrepeval huggingface dataset:
An integrated multi-modal sensor network for video surveillance (2005)

To enhance video surveillance systems, multi-modal sensor integration can be a successful strategy. In this work, a computer vision system able to detect and track people from multiple cameras is integrated with a wireless sensor network mounting PIR (Passive InfraRed) sensors. The two subsystems are briefly described and possible cases in which computer vision algorithms are likely to fail are discussed. Then, simple but reliable outputs from the PIR sensor nodes are exploited to improve the accuracy of the vision system. In particular, two case studies are reported: the first uses the presence detection of PIR sensors to disambiguate between an opened door and a moving person, while the second handles motion direction changes during occlusions. Preliminary results are reported and demonstrate the usefulness of the integration of the two subsystems.
A data description language approach to file translation (1976)

The basic research on data definition languages and the translation algorithm was supported by the Air Force Office of Scientific Research, Air Force Systems Command, U.S.A.F., under Grant No. AFOSR-72-2219. The development and implementation of the Prototype Data Translator was supported by the Joint Technical Support Activity of the Defense Communications Agency under Contract No. DCA 100-72-C-0019. The authors would like to express their appreciation to Janet Eggleton for her assistance in the technical editing and final preparation of this paper.
Chlorosis in ornamentals: Control of lime-induced chlorosis by soil applications of chelated iron can be effective (1953)

Thousands of chlorotic trees and shrubs—on lime soil in southern California—can be made to become green by soil applications of iron-containing chelates.
Protein Synthesis Inhibitors, in Synergy with 5-Azacytidine, Restore Sodium/Iodide Symporter Gene Expression in Human Thyroid Adenoma Cell Line, KAK-1, Suggesting<i>Trans</i>-Active Transcriptional Repressor (2006)

This suggests that trans-active protein factor(s) represses endogenous hNIS transcription in KAK-1 cells under basal conditions, accounting for loss of iodine uptake. Inhibition of this repressive activity increases endogenous hNIS transcription and presents a novel target to restore hNIS expression in dedifferentiated thyroid carcinoma.
Increased expression of matrix metalloproteinase-9 in the eutopic endometrial tissue of women with endometriosis (2006)

These findings make plausible the involvement of MMP-9/TIMP-1 imbalance in the invasiveness of the endometrial tissue of patients with endometriosis and the ectopic development of the disease.
Open-loop tracking of rising and setting GNSS radio-occultation signals from an Airborne Platform: Signal model and statistical analysis (2013)

Global Navigation Satellite System (GNSS) radio occultation (RO) is an atmospheric sounding technique based upon the change in propagation direction of low-elevation GNSS signals through the stratified atmosphere. Atmospheric water vapor profiles can be retrieved from inverting the bending-angle measurements. Open-loop (OL) tracking, in which the received RO signal is cross-correlated with a model signal that does not include refraction effects, is applied to estimate the small excess phase due to atmospheric bending. OL tracking is demonstrated on rising as well as setting satellites observed from an airborne receiver. Setting signals are tracked by processing the sampled signal in reverse, allowing the initialization of tracking after the satellite has reached a higher elevation. A model has been developed to relate the signal to noise ratio (SNR) of the complex correlation to the variance in the residual phase estimate and is used to set a threshold on the minimum SNR for OL tracking. This model is shown to agree well with experimental measurements.
The Tumor Suppressor PTEN Negatively Regulates Insulin Signaling in 3T3-L1 Adipocytes (2000)

PTEN is a tumor suppressor with sequence homology to protein-tyrosine phosphatases and the cytoskeleton protein tensin. PTEN is capable of dephosphorylating phosphatidylinositol 3,4, 5-trisphosphate in vitro and down-regulating its levels in insulin-stimulated 293 cells. To study the role of PTEN in insulin signaling, we overexpressed PTEN in 3T3-L1 adipocytes approximately 30-fold above uninfected or control virus (green fluorescent protein)-infected cells, using an adenovirus gene transfer system. PTEN overexpression inhibited insulin-induced 2-deoxy-glucose uptake by 36%, GLUT4 translocation by 35%, and membrane ruffling by 50%, all of which are phosphatidylinositol 3-kinase-dependent processes, compared with uninfected cells or cells infected with control virus. Microinjection of an anti-PTEN antibody increased basal and insulin stimulated GLUT4 translocation, suggesting that inhibition of endogenous PTEN function led to an increase in intracellular phosphatidylinositol 3,4,5-trisphosphate levels, which stimulates GLUT4 translocation. Further, insulin-induced phosphorylation of downstream targets Akt and p70S6 kinase were also inhibited significantly by overexpression of PTEN, whereas tyrosine phosphorylation of the insulin receptor and IRS-1 or the phosphorylation of mitogen-activated protein kinase were not affected, suggesting that the Ras/mitogen-activated protein kinase pathway remains fully functional. Thus, we conclude that PTEN may regulate phosphatidylinositol 3-kinase-dependent insulin signaling pathways in 3T3-L1 adipocytes.
Herpes simplex virus type 1 origin-dependent DNA replication in insect cells using recombinant baculoviruses (1992)

The minimal set of seven herpes simplex virus type 1 (HSV-1) genes required for viral origin-dependent DNA synthesis was previously identified using a transient replication assay in a mammalian cell line permissive for HSV-1 growth. We have constructed recombinant baculoviruses which efficiently express the products of each of these seven genes in infected Spodoptera frugiperda (Sf) insect cells. When Sf cells were transfected with a plasmid containing a functional HSV-1 origin of replication, and subsequently superinfected with a mixture of these seven viruses, the input plasmid was amplified. This amplification exhibited properties characteristic of genuine HSV-1 DNA replication: all seven HSV-1 replication gene products were required, replicated DNA was detected as concatemers, and mutated origins were impaired to similar extents in insect cells and cells permissive for HSV-1 replication. These results demonstrate that the HSV-1 proteins expressed in Sf cells are fully competent for viral DNA synthesis, and indicate that any host function essential in mammalian cells must also be present in the infected insect cells. This system also provides a convenient method by which mutated replication proteins can be screened for function and produced in amounts sufficient for biochemical studies. Using this approach we show that the ability of the UL9 protein to bind to the viral origins of replication is not sufficient for it to facilitate DNA synthesis.
Design of a DSP-based code-phase-shift keying modem for wireless local area network applications (2002)

We present the design of a receiver which employs code-phase-shift keying, an M-ary direct-sequence spread-spectrum technique in which each of the M signaling waveforms is obtained by a different code phase shift of a single pseudonoise sequence. The tracking and acquisition schemes are adapted from conventional DS-SS; while the carrier-wave synchronization problem is solved by a novel phase-invariant reception technique. The design is tested by a DSP-based implementation. Measurement results are compared with theoretical predictions.
First record of 'Apaturina erminea' (Cramer) (Lepidoptera: Nymphalidae) from Australia (1981)

A single male of 'Apaturina erminea' is recorded from Iron Range, N. Queensland, this being the first specimen taken in Australia. The subspecific status of the specimen is uncertain.
Control architecture for an adaptive electronically steerable flash lidar and associated instruments (2014)

An Electronically Steerable Flash Lidar (ESFL), developed by Ball Aerospace & Technologies Corporation, allows realtime adaptive control of configuration and data-collection strategy based on recent or concurrent observations and changing situations. This paper reviews, at a high level, some of the algorithms and control architecture built into ESFL. Using ESFL as an example, it also discusses the merits and utility such adaptable instruments in Earth-system studies.
Topology-aware task mapping for reducing communication contention on large parallel machines (2006)

Communication latencies constitute a significant factor in the performance of parallel applications. With techniques such as wormhole routing, the variation in no-load latencies became insignificant, i.e., the no-load latencies for far-away processors were not significantly higher (and too small to matter) than those for nearby processors. Contention in the network is then left as the major factor affecting latencies. With networks such as fat-trees of hypercubes, with number of wires growing as P log P, even this is not a very significant factor. However, for torus and grid networks now being used in large machines such as BlueGene/L and the Cray XT3, such contention becomes an issue. We quantify the effect of this contention with benchmarks that vary the number of hops traveled by each communicated byte. We then demonstrate a process mapping strategy that minimizes the impact of topology by heuristically minimizing the total number of hop-bytes communicated. This strategy, and its variants, are implemented in an adaptive runtime system in Charm++ and adaptive MPI, so it is available for a broad class of applications.
High‐gradient magnetic affinity separation of trypsin from porcine pancreatin (2002)

We introduce a robust and scale-flexible approach to macromolecule purification employing tailor-made magnetic adsorbents and high-gradient magnetic separation technology adapted from the mineral processing industries. Detailed procedures for the synthesis of large quantities of low-cost defined submicron-sized magnetic supports are presented. These support materials exhibit unique features, which facilitate their large-scale processing using high magnetic field gradients, namely sufficiently high magnetization, a relatively narrow particle size distribution and ideal superparamagnetism. Following systematic optimization with respect to activation chemistry, spacer length and ligand density, conditions for preparation of effective high capacity (Q(max) = 120 mg g(-1)) strongly interacting (Kd < 0.3 microm) trypsin-binding adsorbents based on immobilized benzamidine were established. In small-scale studies approximately 95% of the endogenous trypsin present in a crude porcine pancreatin feedstock was recovered with a purification factor of approximately 4.1 at the expense of only a 4% loss in alpha-amylase activity. Efficient recovery of trypsin from the same feedstock was demonstrated at a vastly increased scale using a high-gradient magnetic separation system to capture loaded benzamidine-linked adsorbents following batch adsorption. With the aid of a simple recycle loop over 80% of the initially adsorbed trypsin was recovered in-line with an overall purification factor of approximately 3.5.
Detection of Rifampin Resistance in <i>Mycobacterium tuberculosis</i> in a Single Tube with Molecular Beacons (2001)

Current clinical assays for determining antibiotic susceptibility in Mycobacterium tuberculosis require many weeks to complete due to the slow growth of the bacilli. Here we demonstrate an extremely sensitive single-tube PCR assay that takes less than 3 h and reliably identifies rifampin-resistant M. tuberculosis in DNA extracted directly from sputum. Ninety-five percent of mutations associated with rifampin resistance occur in an 81-bp core region of the bacterial RNA polymerase gene, rpoB. All mutations that occur within this region result in rifampin resistance. The assay uses novel nucleic acid hybridization probes called molecular beacons. Five different probes are used in the same reaction, each perfectly complementary to a different target sequence within the rpoB gene of rifampin-susceptible bacilli and each labeled with a differently colored fluorophore. Together, their target sequences encompass the entire core region. The generation of all five fluorescent colors during PCR amplification indicates that rifampin-susceptible M. tuberculosis is present. The presence of any mutation in the core region prevents the binding of one of the molecular beacons, resulting in the absence of one of the five fluorescent colors. When 148 M. tuberculosis clinical isolates of known susceptibility to rifampin were tested, mutations associated with rifampin resistance were detected in 63 of the 65 rifampin-resistant isolates, and no mutations were found in any of the 83 rifampin-susceptible isolates. When DNA extracted directly from the sputum of 11 patients infected with rifampin-resistant tuberculosis was tested, mutations were detected in all of the samples. The use of this rapid assay should enable early detection and treatment of drug-resistant tuberculosis in clinical settings.
The Epididymal Fat Pad as a Transplant Site for Minimal Islet Mass (2007)

The epididymal fat pad was evaluated as a site of islet transplantation in a syngeneic murine model of diabetes by comparing the transplant outcomes to that of islets transplanted intraportal. Mouse islets engrafted on the intra-abdominal epididymal fat pad ameliorated streptozotocin-induced hyperglycemia with similar efficacy as grafts implanted intraportally. Mice that received as few as 50 islets, either intraportal or in the epididymal fat pad, displayed similar glucose tolerance curves. Bioluminescence imaging and glucose measurement showed stable luminescence signals and blood glucose levels for over 5 months in both transplant sites using transgenic luciferase-positive islets. Prompt recurrent hyperglycemia occurred in all mice after removal of the epididymal fat pad bearing the islet graft. Histological examination of the grafts showed well-granulated insulin containing cells surrounded by healthy adipocytes. This study indicates that the epididymal fat pad maybe a useful islet transplant site in the mouse model for effective glycemic control.
Monoclonal Antibody Targeted Delivery of Boron Loaded Gold Nanoparticles to Pancreatic Cancer Cells for Boron Neutron Capture Therapy. (2014)

Pancreatic adenocarcinoma is an aggressive disease with an extremely high mortality rate.New therapies are needed to treat this deadly disease.Gold nanoparticles (GNP) are attractive vehicles for cancer therapeutics.The chemistry of gold allows for easy attachment of therapies.In addition GNP can also access tumors through the leaky vasculature.Boron Neutron Capture Therapy (BNCT) involves localizing non-toxic and non-radioactive Boron-10 to tumor cells.When a boron-10 atom is hit with low thermal energy neutrons it decays into destructive high-energy lithium ions and -particles.By loading boron onto GNP, the therapeutic delivery to tumors can potentially be increased.The success of BNCT also relies on targeting the boron specifically to the tumor in order to localize damage to the tumor.Through attaching an antibody that recognizes a cell surface protein on pancreatic adenocarcinoma cells to the GNP the boron can be directed to the tumor.Here glycoprotein A33 is used as the target.Pancreatic adenocarcinoma cell line BxPC3 does not express A33, while another pancreatic cancer cell line CAPAN2 does.This model allows for the selectivity of boron loaded monoclonal antibody conjugated gold nanoparticles to be evaluated.In order to expose the cells to low thermal energy neutrons they must be transported to MIT Nuclear Reactor Lab, which involves cell culture in CO 2 independent conditions.Culture of cell in CO 2 independent conditions caused no significant morphological changes and importantly A33 expression did not change significantly in BxPC3 and CAPAN2 cell lines.An initial irradiation experiment was performed with monoclonal conjugated boron loaded gold nanoparticles constructs that provides a platform for additional investigation into nanoparticle targeted BNCT.
Development, implantation, <i>in vivo</i> elution, and retrieval of a biocompatible, sustained release subretinal drug delivery system (2005)

A biocompatible, sustained-release subretinal drug-delivery platform was developed to overcome the therapeutic accessibility limitations of current retinal disease treatments. The prototype implants were fabricated by coating nitinol, poly(methyl methacrylate) or chromic gut core filaments, with a drug-eluting polymer matrix. The polymer coatings are manufactured and coated by SurModics. The coating is a mixture of poly(butyl methacrylate) and poly(ethylene-co-vinyl acetate). The drug is either triamcinolone acetonide or sirolimus. The rods were successfully implanted into the subretinal space of 20/24 rabbits. Four rabbits were lost to early surgery from a dysfunctional infusion line and hemorrhage. No serious complications were observed during the 4-week follow-up period. Slight conjunctival redness was reported in all rabbits by 1-day follow-up, but the redness had subsided by the following week. Intraocular lens touch occurred in six rabbits during the implantations; of these, four had a lensectomy at the time of surgery, and the remaining two developed cataract. Corneal edema developed in three rabbits by 1-week follow-up, but subsided within 2 weeks. Initial observations of the implantation and elution characteristics revealed that the implants are well tolerated by the retinal tissue and that the implant can elute triamcinolone acetonide for a period of at least 4 weeks without eliciting an inflammatory response or complications. There were adverse clinical indications with the sirolimus-loaded implants at the delivered dose. Device retrieval required an uncomplicated surgical procedure, and revealed no associated or adherent tissue. Implant drug content analysis and opacity changes to the polymer matrix coating following retrieval demonstrated the sustained elution of the drug.
Inserção do gene PR5K em cana-de-açúcar visando induzir resistência ao fungo da ferrugem Puccinia melanocephala. (2003)

\n A cana-de-açúcar é uma cultura de grande importância econômica para alguns paises da América especialmente para o Brasil. A mesma apresenta grandes problemas de ataque de patógenos e dentre eles destaca-se a ferrugem causada pelo fungo Puccinia melanocephala. O objetivo deste trabalho foi inserir um gene de resistência ao patógeno através da técnica de biolística. Calos embriogênicos de cana-de-açúcar da variedade australiana Q117 sensível à ferrugem, foram bombardeados com partículas de tungstênio revestidas com o vetor pRGA. Este vetor foi construído empregando-se o plasmídio pAHC17 que contém o gene da ubiquitina do milho ubi-1, que se expressa somente em monocotiledônea, o plasmídio pXL3 que contém o gene de interesse PR5K,relacionado com as PR5 proteínas, envolvida no sistema de defesa contra microorganismos patogênicos, e o plasmídio pAH9 que contém o gene neo, que confere resistência ao antibiótico geneticina. A seleção foi feita com o antibiótico geneticina. O plasmídio pRGA foi bombardeado em calos de cana-de-açúcar com 19 semanas, cultivadas em manitol e sorbitol 4 horas antes do bombardeamento. Após o bombardeamento, os calos foram transferidos para meio CI-3 onde ficaram no escuro por 10 dias. Os mesmos foram transferidos para meio CI-3-2,4-D de regeneração contendo o antibiótico de seleção geneticina (35 mg/mL). Os calos selecionadas foram repicadas para meio de cultivo CI-3BAP. As plântulas regeneradas apresentaram um percentual de 20%. As mesmas foram submetidas à análise de confirmação por PCR, empregando-se os primers D12 e E01. Pela analise do resultado do PCR, foi observado que cerca de 1% apresentou banda correspondente ao pRGA indicando possível transformação, e que várias regiões foram amplificadas em relação às analisadas.\n
Functional Analysis of the <i>Lactococcus lactis galU</i> and <i>galE</i> Genes and Their Impact on Sugar Nucleotide and Exopolysaccharide Biosynthesis (2001)

We studied the UDP-glucose pyrophosphorylase (galU) and UDP-galactose epimerase (galE) genes of Lactococcus lactis MG1363 to investigate their involvement in biosynthesis of UDP-glucose and UDP-galactose, which are precursors of glucose- and galactose-containing exopolysaccharides (EPS) in L. lactis. The lactococcal galU gene was identified by a PCR approach using degenerate primers and was found by Northern blot analysis to be transcribed in a monocistronic RNA. The L. lactis galU gene could complement an Escherichia coli galU mutant, and overexpression of this gene in L. lactis under control of the inducible nisA promoter resulted in a 20-fold increase in GalU activity. Remarkably, this resulted in approximately eightfold increases in the levels of both UDP-glucose and UDP-galactose. This indicated that the endogenous GalE activity is not limiting and that the GalU activity level in wild-type cells controls the biosynthesis of intracellular UDP-glucose and UDP-galactose. The increased GalU activity did not significantly increase NIZO B40 EPS production. Disruption of the galE gene resulted in poor growth, undetectable intracellular levels of UDP-galactose, and elimination of EPS production in strain NIZO B40 when cells were grown in media with glucose as the sole carbon source. Addition of galactose restored wild-type growth in the galE disruption mutant, while the level of EPS production was approximately one-half the wild-type level.
Resolution Enhancement of Photoacoustic FTIR Spectra (1985)

Fourier self-deconvolution of a photoacoustic FTIR spectrum by direct manipulation of the observed interferogram is shown to be possible. Over a narrow frequency range, the measured photoacoustic (PA) signal is approximately proportional to the optical absorption coefficient, thereby enabling known line shapes to be used in the deconvolution. The method is applied to the PA spectrum of kaolinite and the enhanced spectrum is compared with other theoretical and experimental results.
A Quantitative Description of the Binding Equilibria of para-Substituted Aniline Ligands and CdSe Quantum Dots (2010)

This paper describes the use of 1H NMR spectroscopy to measure the equilibrium constants for the solution-phase binding of two para-substituted aniline molecules (R-An), p-methoxyaniline (MeO-An) and p-bromoaniline (Br-An), to colloidal 4.1 nm CdSe quantum dots (QDs). Changes in the chemical shifts of the aromatic protons located ortho to the amine group on R-An were used to construct a binding isotherm for each R-An/QD system. These isotherms fit to a Langmuir function to yield Ka, the equilibrium constant for binding of the R-An ligands to the QDs; Ka ≈ 150 M−1 and ΔGads ≈ −19 kJ/mol for both R = MeO and R = Br. 31P NMR indicates that the native octylphosphonate ligands, which, by inductively coupled plasma atomic emission spectroscopy, cover 90% of the QD surface, are not displaced upon binding of R-An. The MeO-An ligand quenches the photoluminescence of the QDs at much lower concentrations than does Br-An; the observation, therefore, that Ka,MeO-An ≈ Ka,Br-An shows that this difference in quenching efficiencies is due solely to differences in the nature of the electronic interactions of the bound R-An with the excitonic state of the QD.