gebhart/patrepeval huggingface dataset:
Cloning, sequencing, and expression in Escherichia coli of a Streptococcus faecalis autolysin (1991)

A Streptococcus faecalis genomic bank was obtained by partial digestion with MboI and cloning into the SalI restriction site of pTZ18R. Screening of about 60,000 Escherichia coli transformants for cell wall lysis activity was done by exposing recombinant colonies grown on medium containing lyophilized Micrococcus lysodeikticus cells to chloroform and toluene vapors in order to release proteins. Because this procedure provoked cell death, colonies could not be used directly for transformant recovery; however, recovery was achieved by partial purification of plasmid DNA from active colonies on the agar plate and transformation of E. coli competent cells. About 60 recombinants were found. One of them (pSH6500) codes for a lytic enzyme active against S. faecalis and M. lysodeikticus cell walls. A shorter clone (pSH4000) was obtained by deleting an EcoRI fragment from the 6.5-kb original insert, leaving a 4-kb EcoRI-MboI insert; this subclone expressed the same lytic activity. Sequencing of a portion of pSH4000 revealed a unique open reading frame of 2,013 nucleotides coding for a 641-amino-acid (74-kDa) polypeptide and containing four 204-nucleotide direct repeats.
Enhancement of erythropoiesis <i>in vitro</i> by human growth hormone is mediated by insulin‐like growth factor I (1988)

Insulin-like growth factor I (IGF-I) is the presumed paracrine or autocrine growth-promoting mediator of growth hormone in peripheral tissues. In order to evaluate the role of IGF-I as mediator of human growth hormone (hGH) in erythropoiesis, we compared the effects of both peptides upon in vitro colony formation by primitive (BFU-E) and relatively mature (CFU-E) human erythroid precursors. Biosynthetic IGF-I (2 ng/ml) and hGH (25 ng/ml) induced a significant increase in the growth of both BFU-E and CFU-E. BFU-E growth was maximally enhanced by 6 ng/ml IGF-I and by 50 ng/ml hGH, resulting in an increase in burst numbers of 62 +/- 12% and 52 +/- 12%, respectively. Maximal enhancement of CFU-E growth was detected at higher concentrations of IGF-I (20 ng/ml) and hGH (150 ng/ml), with respective increases of 121 +/- 35% and 137 +/- 18% in colony numbers. Enhancement of bone marrow and peripheral blood erythroid progenitor cell growth by hGH required the presence of monocytes and was abrogated by specific monoclonal antibodies directed against IGF-I membrane receptors. The in vitro growth-promoting effect of hGH upon human erythroid precursors thus appears to be mediated by paracrine IGF-I.
Realization of a low-voltage and low-power Colpitts quadrature oscillator (2006)

LSI2
Immunodiagnosis of schistosomiasis patients in The Netherlands: comparison of antibody and antigen detection before and after chemotherapy. (1990)

We investigated in a retrospective study the application of an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of the schistosome circulating anodic antigen (CAA) to the diagnosis of schistosomiasis in our laboratory. CAA-titres were compared with IgG-titres as determined with an ELISA using soluble egg antigen (SEA) and IgM-titres as determined in an immuno fluorescence assay (IFA) on sections of Rossman's fixed schistosomes. Pre- and post-treatment serum samples of 182 individuals, suspected for schistosomiasis, were used for this study, including 100 control samples. CAA was detected in 20.7% of all patients who were positive in either IFA or SEA-ELISA or both. CAA-titres did not correlate with either IgM-titres or IgG-titres. No significant drop in IgG level was found after specific treatment (Wilcoxon's P = 0.35). In contrast, a significant fall in both IgM-titre (P less than 0.01), and CAA-level (P less than 0.01) was observed. All CAA-titres became negative after chemotherapy.
[Results of international clinical trials with raloxifene]. (1999)

A new drug class called Selective Estrogen Receptor Modulators (SERM) could combine ideal properties for a product designed for menopausal women. The most widely studied member of this class is raloxifene which is currently marketed in several countries for the prevention of osteoporosis in menopaused women. This product is a nonsteroidal derivative of benzothiophene which, like estrogens, has a preventive effect against bone loss involving the spine and peripheral skeleton and a cholesterol lowering effect, both in the ovariectomized rat and in menopausal women. Unlike estrogens, raloxifene does not stimulate breast or uterine tissue. These interesting properties make raloxifene a possible preventive treatment for osteoporosis and other menopause-related risks for menopausal women of all ages. Multicenter studies have been conducted in recently menopausal women who received either raloxifene at the doses of 30, 60, or 150 mg/day or a placebo in a randomized protocol. All subjects were also given calcium supplementation. Bone density was measured twice a year for 36 months by dual X-rays absorptiometry and showed a significant decrease at all sites in the placebo group while there was a significant increase in the spine, the hip and the overall skeleton for all three raloxifen groups. After 24 months of treatment, mean increase over placebo was 2.4% for 60 mg raloxifene measured on the spine and total hip and 2% for the overall skeleton. Markers of bone formation (serum osteocalcin and bone alkaline phasphatase) and resorption (urinary CrossLaps) decreased significantly reaching, after 3 to 6 months of treatment, the levels observed in non menopausal women. In addition, total serum cholesterol as well as LDL-cholesterol decreased significantly in a dose-dependent fashion in all groups treated with raloxifene. Serum HDL-cholesterol and triglycerides did not very significantly during treatment. Hot flashes were the most frequently observed undesirable effect, at a frequency slightly higher in the raloxifene group (25%) than in the placebo group (18%). This undesirable effect was of low intensity and generally occurred during the first months of treatment. It did not cause a higher drop out rate (raloxifen 1.5%; placebo 2.1%). The preliminary data at two years follow-up suggest that raloxifene is not associated with an increased risk of breast cancer. In conclusion, raloxifene is a particularly interesting drug for menopausal women showing very promising efficacy and clinical tolerance.
Analysis of packet reservation multiple access: voice data integration for wireless networks (2002)

It is shown that PRMA (packet-reservation multiple-access) gracefully accepts low-rate data terminals with moderate data packet delays. Due to the two classes of terminals, calculation of system equilibrium requires the solution of two simultaneous nonlinear equations. By a judicious change of variables, this is reduced to a univariate problem of the same form as the speech-only system. Results for a particular design example show that, with a small reduction in the number of speech terminals, all terminals can be provided with a low-rate data capability (1200/2400 b/s) with data packet delays of a few hundred milliseconds. The results are of interest in connection with the development of future microcellular systems which will require distributed network control.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Tools for modelling the kinematics of multi-body systems (1983)

The kinematic behaviour of robots, an important subset of multibody systems, is described by highly nonlinear equations. Due to the 3-D movements and nonlinearities of the connections, simulation and optimization software has to be developed for analyses. Currently available software restricts the user to use simple connections. Some advanced tools are presented for modelling the kinematics of multibody systems with arbitrary connections. Both the simulation and optimization problems are formulated and attention is paid to appropriate numerical solvers
Fatty acid and lipid compositions of <i>Conidiobolus</i> (1983)

The fatty acid composition of the total lipids from two Conidiobolus species was studied by gas—liquid chromatography. The major fatty acids of C. lamprauges were palmitic acid (C 16:0 ), oleic acid (C 18:1 ), linolenic acid (C 18:3 ), and arachidonic acid (C 20:4 ). For C. eurymitus , myristic acid (C 14:0 ), C 16:0 , and linoleic acid (C 18:2 ) were the most abundant acids. The fatty acid composition of C. eurymitus was quite different from that of the Conidiobolus species as mentioned in other reports. The lipid composition of the total lipids of C. lamprauges and C. eurymitus was also studied by thinchrography on quartz rods. Triglycerides and phospholipids were the major components in the two Conidiobolus species.
A child’s perspective (2013)

Trapped in the darkened crowd I thought I was going to die. The noise beat away at my insides and the jostling children felt like an assault. I could see no way out and no way to make it stop. I was invisible, naked; drowning in a world that was merciless and hateful. There was no escape.
Impact of time to therapy and reperfusion modality on the efficacy of adenosine in acute myocardial infarction: the AMISTAD-2 trial (2006)

In this post hoc analysis, 3 h adenosine infusion administered as an adjunct to reperfusion therapy within the first 3.17 h onset of evolving anterior ST-segment elevation AMI enhanced early and late survival, and reduced the composite clinical endpoint of death or CHF at 6 months.
Separation of <i>Escherichia coli</i> Bacteria from Peripheral Blood Mononuclear Cells Using Standing Surface Acoustic Waves (2013)

A microfluidic device was developed to separate heterogeneous particle or cell mixtures in a continuous flow using acoustophoresis. In this device, two identical surface acoustic waves (SAWs) generated by interdigital transducers (IDTs) propagated toward a microchannel, which accordingly built up a standing surface acoustic wave (SSAW) field across the channel. A numerical model, coupling a piezoelectric effect in the solid substrate and acoustic pressure in the fluid, was developed to provide a better understanding of SSAW-based particle manipulation. It was found that the pressure nodes across the channel were individual planes perpendicular to the solid substrate. In the separation experiments, two side sheath flows hydrodynamically focused the injected particle or cell mixtures into a very narrow stream along the centerline. Particles flowing through the SSAW field experienced an acoustic radiation force that highly depends on the particle properties. As a result, dissimilar particles or cells were laterally attracted toward the pressure nodes at different magnitudes, and were eventually switched to different outlets. Two types of fluorescent microspheres with different sizes were successfully separated using the developed device. In addition, Escherichia coli bacteria premixed in peripheral blood mononuclear cells (PBMCs) were also efficiently isolated using the SSAW-base separation technique. Flow cytometric analysis on the collected samples found that the purity of separated E. coli bacteria was 95.65%.
Collusion of fingerprints via the gradient attack (2005)

The difficulty of building an effective digital rights management system stems from the fact that traditional cryptographic primitives such as encryption or scrambling do not protect audio or video signals once they are played in plain-text. This fact, commonly referred to as "the analog hole," has been responsible for the popularity of multimedia file sharing which cannot be controlled, at least technically, by content's copyright owners. In this paper, we explore a specific issue in multimedia fingerprinting as an answer to "the analog hole" problem. We analyze the collusion resistance of spread-spectrum fingerprints with an arbitrary probability distribution of their source using a recently introduced collusion procedure, the gradient attack
Technologies for building networked collaborative environments (2003)

In this paper, we begin by reporting our work in lip synchronization. We then expand on this technology to introduce our recent effort in developing a networked collaborative environment that integrates image analysis, face animation, and directional sound in order to provide a truly immersive environment, with the goal of replacing existing video conferencing platforms. A related technology, streaming of 3D objects, is also introduced.
Concept of vertical bipolar transistor with lateral drift region, applied to high voltage SiGe HBT (2012)

We demonstrate the increase of available collector emitter voltage of integrated vertical bipolar transistors by means of an additional lateral drift region introduced between sub collector and collector contact region. The chosen approach enables the fabrication of high voltage bipolar transistors for RF power applications alternatively to the construction of deep collector wells in vertical direction by an extra epitaxy step or ion implantation with very high energy. The new approach was verified with a modified standard SiGe:C HBT integrated in a high performance BiCMOS process. After introduction of an additional lateral drift region with a length of 1.2 μm BV <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">CE0</sub> of the HBT has increased from 7 V to 18 V.
Effective Training Data Improved Ensemble Approaches for Urinalysis Model (2011)

Urinalysis remains one of the most commonly performed tests in clinical practice. Laboratory work can be greatly relieved by automated analyzing techniques. However, noisy and imbalanced urine samples make automatically identifying and classifying urine-related diseases become very difficult. This paper proposed hybrid sampling-based ensemble learning strategies by improving training data and classification performance. Having compared the effectiveness of several learning classifiers and data processing techniques, the experiments showed that the suggesting methods provided better classification accuracy than other approaches.
A perfluorinated anion exchange membrane with a 1,4-dimethylpiperazinium cation (2011)

A perfluorinated anion exchange membrane with a 1,4-dimethylpiperazinium cation demonstrated better hydroxide ion conductivity and three-fold reduction in water uptake compared to a hydrocarbon AEM with the same cation. The perfluorinated AEM was stable over 30 days in 2 M KOH at 60 °C and demonstrated good fuel cell performance.
Theology and Culture (1978)

There is a moment in Twelfth Night when Feste, the Fool, calls upon Malvolio, who has been shut up in a dark cell as a dangerous madman. Feste, to render himself the more credible as a prison visitor, puts on a parson’s gown and a parson’s voice and, announcing that he is Sif Topas the curate who comes to visit Malvolio the lunatic, counsels him with words of learned but wholly irrelevant comfort. Malvolio, in frustration at this pastoral nonsense, most violently asserts that he is in no way mad, so the pretence clergyman agrees to test the prisoner’s wits by posing some deep question. ‘What’, he asks, ‘is the opinion of Pythagoras concerning wild fowl?’ From that encyclopaedic knowledge of the classical world we expect in a renascence man, Malvolio answers that the philosopher held ‘that the soul of our grandam might haply inhabit a bird’. Ruthless as if he were conducting a seminar, the Fool then asks him: ‘What think’st thou of his opinion?’ Malvolio rather carefully replies: T think nobly of the soul and no way approve his opinion’. But at this the clergyman seems quite convinced that Malvolio is indeed mad, declaring as he hurries away, ‘thou shalt hold th’opinion of Pythagoras ere I will allow of thy wits’. This absurd interlude seems to many, I suspect, a paradigm of theological talk. A madman and a fool disputing about the soul, and as if this were not itself at sufficient remove from reality, disputing about whether the human soul might animate a bird. I should like to suggest to you, however, that while this dialogue may very well present an unkind parody of the manners both of the student of religion and of the believer, its satire does not, even glancingly, touch the theologian’s enterprise.
REINING IN RIPENING (2007)

RETURN TO ISSUEPREVNewsNEXTREINING IN RIPENINGResearchers are learning to control key biochemical processes that affect the quality of FRUITS AND VEGETABLESSARAH EVERTSView Author Information C&EN BERLINCite this: Chem. Eng. News 2007, 85, 44, 10–15Publication Date (Print):October 29, 2007Publication History Published online29 October 2010Published inissue 29 October 2007https://pubs.acs.org/doi/10.1021/cen-v085n044.p010https://doi.org/10.1021/cen-v085n044.p010newsACS PublicationsCopyright © 2007 AMERICAN CHEMICAL SOCIETYArticle Views13Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Plant derived food Get e-Alerts
Integrated silicon photoconductors for picosecond pulsing and gating (1984)

Integrated photoconductors were constructed on a crystalline Si substrate through standard integrated circuit fabrication techniques followed by shadow-masked ion-beam irradiation. Optoelectronic cross-correlation measurements were performed on these structures with a femtosecond colliding-pulse mode-locked dye laser system. Photoconductors processed as pulsers produced <2-ps rise time ∼ 200-mV pulses with full width at half maxima (FWHM) of 20 ps. Photoconductors processed as sampling gates demonstrated 3-dB measurement bandwidths between 5.3 and 7.6 GHz. Due to the absence of jitter, on-chip signal delays were measured with sub-picosecond precision.
A new design paradigm for the rapid development of haptic and telehaptic applications (2005)

The concept of modern day haptics - providing the simulated sense of touch - has been a topic of research since the early 1980's. Since then, a number of researchers and companies have striven to drive haptic applications into the mainstream. One of the primary barriers to the development of commercially viable application is the disconnection between the application visionaries, which tend not to be haptics experts, and the available development tools. In this paper, we discuss a new paradigm for haptic and telehaptic application development The basis of this new paradigm is a drag and drop, block diagram based design environment Thus, the application developers, which tend to have industry specific expertise but rarely haptic knowledge, are able to independently prototype haptic and telehaptic applications with minimal software coding. This new paradigm promises to vastly improve prototyping efficiency and enable a much larger group of application developers
Channel capacity of charge-constrained run-length limited codes (1981)

The methods of information theory are applied to run-length limited codes with charge constraints. These self-clocking codes are useful in several areas of information storage, including magnetic recording, where it may be desirable to eliminate the dc component of the frequency spectrum. The channel capacity of run-length limited codes, with and without charge constraints, is derived and tabulated. The channel capacity specifies the maximum ratio of data/message bits achievable in implementing these codes and gives insight into the choice of codes for a particular task. The well-known frequency modulation (FM) code provides a simple example of these techniques.
Morphologic conversion of a neuroblastoma-derived cell line by E6-mediated p53 degradation. (2001)

Neuroblastoma-derived tumor cells, unlike cells from other tumor types, characteristically express a wildtype but cytoplasmically sequestered p53 protein. To ascertain whether the p53 in these cells retained any physiological activity, we inactivated it in SK-N-SH cells, a neuroblastoma-derived cell line, by introducing the human papilloma virus type 16 E6 expression plasmid. Parent SK-N-SH cell cultures are composed of two cell types exhibiting characteristic morphologies designated neuroblastic (N-type) or substrate-adherent fibroblastic (S-type) cells, both of which have been shown to spontaneously transdifferentiate or interconvert. We report here that down-regulation of p53 resulted in conversion of SK-N-SH cells to the substrate-adherent fibroblast-like S-type cells. The morphologic conversion was accompanied by a loss of neurofilament expression, a marker for the neuronal N-type cells, an increase in the expression of vimentin, and a lack of responsiveness to retinoic acid-induced neuronal differentiation. Importantly, we did not observe N-type cells in the E6-transfected cell population, suggesting that they were incapable of transdifferentiating to the N-type morphology. We also tested the ability of these E6-transfected S-type cells to form colonies in soft agar and observed a markedly reduced capacity of these cells to do so when compared with the parent and mutant E6-transfected cells. These results suggest that p53 is required for the maintenance of the neuroblastic tumorigenic phenotype.
EXPRESSION OF THE MULTDORUG RESISTANCE GLYCOPROTEIN 170 IN THE PERIPHERAL BLOOD LYMPHOCYTES OF RHEUMATOID ARTHRITIS PATIENTS. THE PERCENTAGE OF LYMPHOCYTES EXPRESSING GLYCOPROTEIN 170 IS INCREASED IN PATIENTS TREATED WITH PREDNISOLONE (1996)

The objective was to evaluate the expression of the multidrug resistance P-glycoprotein (P-gp) in peripheral blood lymphocytes (PBL) of patients with rheumatoid arthritis (RA). PBL from 68 RA patients and 44 controls were evaluated. RA patients had a mean disease duration of 10.7 yr, with a mean number of past resistances to DMARDs of 0.82, and were treated with NSAIDs (n = 34), DMARDs (n = 25) and prednisolone (n = 40). Fluorescence flow cytometry was used to assess P-gp membrane expression on PBL. In the RA group, the percentage of PBL expressing P-gp was Higher in patients treated with prednisolone than in other patients [mean ± s.D.: 10.7 ± 15.8% vs 3.3 ± 7.6%, P < 0.03, Student] and was not related to other therapies, age, sex, RA duration, number of past resistances to DMARDs, activity, ESR, CRP. The percentage of PBL expressing P-gp did not differ in RA and control groups, but was higher in the prednisolone-treated RA patients than in controls. Prednisolone could induce a rise in the percentage of PBL expressing P-gp. On the contrary, patients with a high percentage of PBL expressing P-gp could be more resistant to DMARDs and need prednisolone earlier. Further studies are needed to address this question and to evaluate the potential implication of P-gp in drug resistance in RA.