gebhart/patrepeval huggingface dataset:
Topical Ciprofloxacin/Dexamethasone is Superior to Ciprofloxacin Alone in Pediatric Patients with Acute Otitis Media and Otorrhea through Tympanostomy Tubes (2003)

Abstract Objective To determine whether topical administration of a corticosteroid improves resolution of acute tympanostomy tube otorrhea when combined with topical antibiotic drops. Study Design Randomized, patient‐masked, parallel‐group, multicenter trial of topical otic ciprofloxacin/dexamethasone versus topical ciprofloxacin alone in 201 children aged 6 months to 12 years with acute otitis media with tympanostomy tubes (AOMT) of less than or equal to 3 weeks' duration and visible otorrhea. Methods Eligible patients were randomized to receive three drops of either ciprofloxacin 0.3%/dexamethasone 0.1% or ciprofloxacin 0.3% into the affected ear or ears twice daily for 7 days. Clinical signs and symptoms of AOMT were evaluated on days 1 (baseline), 3, 8 (end‐of‐therapy), and 14 (test‐of‐cure), and twice‐daily assessments of otorrhea were recorded in patient diaries. Results The mean time to cessation of otorrhea in the microbiologically culture‐positive patient population ( n = 167) was significantly shorter with topical ciprofloxacin/dexamethasone than with ciprofloxacin alone (4.22 vs. 5.31 days; P = .004). This resulted in significantly better clinical responses on days 3 and 8 ( P < .0001 and P = .0499, respectively). However, there were no significant differences between the two treatment groups in either the clinical response or the microbial eradication rate by day 14. Conclusions Topical otic treatment with ciprofloxacin/dexamethasone is superior to treatment with ciprofloxacin alone and results in a faster clinical resolution in children with AOMT. The contribution of the corticosteroid in achieving a 20% reduction (1.1 day) in time to cessation of otorrhea is clinically meaningful and represents an important advance over single‐agent antibiotic therapy.
<i>Ab Initio</i> Modeling of Protein/Biomaterial Interactions: Glycine Adsorption at Hydroxyapatite Surfaces (2008)

How does glycine adsorb at hydroxyapatite surfaces? Ab initio simulations based on periodic B3LYP GTO calculations reveal the detailed mechanism of binding to the (001) and (010) surfaces by shedding light on how acid and basic amino acid residues of proteins interact with hydroxyapatite based biomaterials.
Comparison between clinical evaluation and ultrasonography in detecting hydrarthrosis of the knee. (1999)

Clinical knee hydrarthrosis detection is less accurate and reproducible than US detection.
Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity (2007)

Faithful chromosome segregation in mitosis requires the formation of a bipolar mitotic spindle with stably attached chromosomes. Once all of the chromosomes are aligned, the connection between the sister chromatids is severed by the cysteine protease separase. Separase also promotes centriole disengagement at the end of mitosis. Temporal coordination of these two activities with the rest of the cell cycle is required for the successful completion of mitosis. In this study, we report that depletion of the microtubule and kinetochore protein astrin results in checkpoint-arrested cells with multipolar spindles and separated sister chromatids, which is consistent with untimely separase activation. Supporting this idea, astrin-depleted cells contain active separase, and separase depletion suppresses the premature sister chromatid separation and centriole disengagement in these cells. We suggest that astrin contributes to the regulatory network that controls separase activity.
A historical perspective of speech recognition (2014)

What do we know now that we did not know 40 years ago?
Adaptive fuzzy c-shells clustering and detection of ellipses (1992)

Several generalizations of the fuzzy c-shells (FCS) algorithm are presented for characterizing and detecting clusters that are hyperellipsoidal shells. An earlier generalization, the adaptive fuzzy c-shells (AFCS) algorithm, is examined in detail and is found to have global convergence problems when the shapes to be detected are partial. New formulations are considered wherein the norm inducing matrix in the distance metric is unconstrained in contrast to the AFCS algorithm. The resulting algorithm, called the AFCS-U algorithm, performs better for partial shapes. Another formulation based on the second-order quadrics equation is considered. These algorithms can detect ellipses and circles in 2D data. They are compared with the Hough transform (HT)-based methods for ellipse detection. Existing HT-based methods for ellipse detection are evaluated, and a multistage method incorporating the good features of all the methods is used for comparison. Numerical examples of real image data show that the AFCS algorithm requires less memory than the HT-based methods, and it is at least an order of magnitude faster than the HT approach.
Design protocols in triarylamine cored dendrimer-based explosive sensors (2012)

The preparation, photophysical characterisation and explosive sensing of two first-generation triarylamine-based dendrimers are reported. The dendrimers are constructed of a triphenylamine core, dendrons comprised of a phenyl branching unit with thiophene (Dendrimer 1) or bithiophene (Dendrimer 2) moieties, and dodecyl surface groups. By simply changing the number of thiophene units within the dendrons, it was found that the emissive chromophore could be switched from the centre (Dendrimer 1) to the dendrons (Dendrimer 2). Both Dendrimer 1 and 2 detected nitro-aromatic and -aliphatic analytes by fluorescence quenching, with the former showing a much greater response. In particular, Dendrimer 1 showed a particularly high affinity for the difficult-to-detect nitroaliphatic taggant 2,3-dinitro-2,3-dimethylbutane (DMNB). A combination of steady-state and time-resolved Stern–Volmer measurements revealed that the high affinity for DMNB was due to a combination of long luminescence lifetime and dominant static quenching.
Different mechanisms for inhibition of cell proliferation via cell cycle proteins in PC12 cells by nerve growth factor and staurosporine (1997)

PC12 cells have previously been shown to cease cell division during nerve growth-factor (NGF)-induced differentiation by affecting specific cell cycle proteins. Staurosporine, a protein kinase inhibitor, also causes PC12 cell differentiation, independently of neurotrophins or plasma membrane receptors. We have investigated the relationship of the tumor suppressor protein, p53, and other cell cycle proteins to the antiproliferative effects of NGF and staurosporine in PC12 cells. NGF treatment of PC12 cells stimulated an increase of p53 protein in the nucleus and, more slowly, an increase in total cellular p53 protein. Levels of the cyclin-kinase inhibitor p21/WAF1, cyclin D1, and cyclin G, all downstream transcriptional targets of p53, increased after short times of NGF treatment. Cessation of replication and differentiation occurred more rapidly in defined medium (2 days) than in serum medium (6 days), in correspondence with the more rapid changes in both p53 and p21/WAF1 levels in defined medium (1 hour) than in serum (1 day). Levels of p34cdc2 and p33cdk2 kinase dropped after 6 to 10 days treatment with NGF in serum, close to the time of terminal differentiation. Staurosporine, on the other hand, inhibited DNA replication of PC12 cells in a time- and dose-dependent fashion by affecting cyclin-dependent kinases. Staurosporine had no effect on the protein levels of p53, p21/WAF1, or cyclin G. The kinase activity of both p34cdc2 and p33cdk2 were inhibited in vitro with IC50 values of 20 nM and 75 nM, respectively. In vivo p34cdc2 kinase activity was inhibited within 1 day, before the decrease in the levels of p34cdc2 protein at days 2 to 3. In contrast, in vivo p33cdk2 kinase activity only decreased in concert with protein levels. Although both NGF and staurosporine inhibit DNA replication concomitant with induction of differentiation by affecting the activity of p34cdc2 and p33cdk2, the mechanism of the two agents is quite different. NGF achieves inhibition of activity of these cyclin-dependent kinases by signalling through the TrkA receptor to the tumor supressor protein p53 and then to p21/WAF1. In contrast, staurosporine directly inhibits the activity of p34cdc2 and p33cdk2 by binding to them and also indirectly by alteration of their phosphorylation through other regulatory kinases. J. Neurosci. Res. 49:461–474, 1997. © 1997 Wiley-Liss, Inc.
Occupant Position Sensing Systems: Functional Requirements and Technical Means (1993)

<div class="htmlview paragraph"><b>In this paper we discuss possible applications and design considerations for an Occupant Position Sensing System (OPSS). First, the minimum functional requirements and possible advanced applications for an OPSS are presented. The relation of an OPSS to current and future deployment decision systems is discussed. We then consider the implications of the functional requirements for the choice of technology. Finally, we describe a prototype occupant position sensor, and display its position and motion tracking capability.</b></div>
Rapid in situ hybridization technique using 16S rRNA segments for detecting and differentiating the closely related gram-positive organisms Bacillus polymyxa and Bacillus macerans (1992)

A rapid, sensitive, inexpensive in situ hybridization technique, using 30-mer 16S rRNA probes, can specifically differentiate two closely related Bacillus spp., B. polymyxa and B. macerans. The 16S rRNA probes were labeled with a rhodamine derivative (Texas Red), and quantitative fluorescence measurements were made on individual bacterial cells. The microscopic fields analyzed were selected by phase-contrast microscopy, and the fluorescence imaging analyses were performed on 16 to 67 individual cells. The labeled 16S rRNA probe, POL, whose sequence was a 100% match with B. polymyxa 16S rRNA but only a 60% match with B. macerans 16S rRNA, gave quantitative fluorescence ratio measurements that were 34.8-fold higher for B. polymyxa cells than for B. macerans cells. Conversely, the labeled probe, MAC, which matched B. polymyxa 16S rRNA in 86.6% of its positions and B. macerans 16S rRNA in 100% of its positions, gave quantitative fluorescence measurements that were 59.3-fold higher in B. macerans cells than in B. polymyxa cells. Control probes, whose 16S rRNA sequence segment (P-M) was present in both B. polymyxa and B. macerans as well as a panprokaryotic probe (16S), having a 100% match with all known bacteria, hybridized equally well with both organisms. These latter hybridizations generated very high fluorescence signals, but their comparative fluorescence ratios (the differences between two organisms) were low. The control paneukaryotic probe (28S), which had less than 30% identity for both B. macerans and B. polymyxa, did not hybridize with either organism.
The Study of Mime as a Manifestation of Sociability, as Play and Artistic Expression (1965)

Mime expresses a condition suffered by all men: the physical condition. Since here is the seat of all consciousness and the implicit support of the mind's most delicate constructions, mime is the original and universal language. Learning to talk is mimicry at the beginning, and the small child repeats the words before he has understood their meaning. The adult never stops resorting to it; sympathy, love are born and avowed in a mimetic exchange; and relaxation results from the freedom of movement which most games entail. Mime is also a means of social communication; it gives birth to pity, which is awakened by physical sympathy. Pity in turn is the most binding sentiment of all : Montesquieu, Jean-Jacques Rousseau and anthropology consider it fundamental to the condition of society.
A method of HLA class II typing using non‐radioactive labelled oligonucleotides (1989)

The typing of HLA class II genes using molecular biology techniques has brought undoubtedly new insights in the analysis of their polymorphism. Particularly interesting is the dot-blot analysis of enzymatically-amplified genomic DNA hybridized with sequence-specific oligonucleotides. In order to use this technique of typing on a routine basis, we established a non-radioactive detection method of enzymatically-amplified genomic DNA dot-blots. We could clearly demonstrate that, using biotin-labelled specific oligonucleotides, it was possible to specifically discriminate between DQB1 first domain DNA sequences displaying three, two or even only one base-pair difference at a given codon position. The very satisfactory sensitivity level reached by this non-radioactive detection method could safely allow its use for clinical applications of HLA typing at the DNA level.
Genetic susceptibility to carbamazepine-induced cutaneous adverse drug reactions (2006)

The anticonvulsant carbamazepine (CBZ) frequently causes cutaneous adverse drug reactions (cADRs), including maculopapular eruption (MPE), hypersensitivity syndrome (HSS), Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN). We reported that SJS/TEN caused by CBZ is strongly associated with the HLA-B*1502 gene in Han Chinese. Here, we extended our genetic study to different types of CBZ-cADRs (91 patients, including 60 patients with SJS/TEN, 13 patients with hypersensitivity syndrome and 18 with maculopapular exanthema versus 144 tolerant controls). We used MALDI-TOF mass spectrometry to screen the genetic association of 278 single nucleotide polymorphisms (SNPs), which cover the major histocompatibility complex (MHC) region, tumor necrosis factor-alpha, heat shock protein and CBZ-metabolic enzymes, including CYP3A4, 2B6, 2C8, 2C9, 1A2 and epoxide hydrolase 1. In addition, we genotyped 20 microsatellites in the MHC region and performed HLA-typing to construct the recombinant map. We narrowed the susceptibility locus for CBZ-SJS/TEN to within 86 kb flanking the HLA-B gene on the extended B*1502 haplotype, and confirmed the association of B*1502 with SJS/TEN [Pc=1.6x10, odds ratio (OR)=1357; 95% confidence interval (CI)=193.4-8838.3]. By contrast to CBZ-SJS/TEN, HLA-B*1502 association was not observed in the MPE or HSS groups: MPE was associated with SNPs in the HLA-E region and a nearby allele, HLA-A*3101 (Pc=2.2x10, OR=17.5; 95% CI=4.6-66.5), and HSS with SNPs in the motilin gene (Pc=0.0064, OR=7.11; 95% CI=3.1-16.5) located terminal to the MHC class II genes. No SNPs in genes involved in CBZ metabolism were associated with CBZ-induced cADRs. Our data suggest that HLA-B*1502 could contribute to the pathogenesis of CBZ-SJS/TEN, and that genetic susceptibility to CBZ-induced cADRs is phenotype-specific.
Wave-Equation Migration Velocity Analysis using Focusing of Extended Images (2009)

Wave-equation migration velocity analysis (WEMVA) is a velocity estimation technique designed to invert for velocity information using migrated images. Its capacity for handling multi-pathing makes it appropriate in complex subsurface regions characterized by strong velocity variation. WEMVA operates by establishing a linear relation between a velocity model perturbation and a corresponding migrated image perturbation. The linear relationship is derived from conventional extrapolation operators and it inherits the main properties of frequency-domain wavefield extrapolation. A key step in implementing WEMVA is to design an appropriate procedure for constructing image perturbations. Using time-lag extended images, one can characterize the error in migrated images by defining the focusing error as the shift of the focused reflection along the time-lag axis. Under the linear approximation, the focusing error can be transformed into an image perturbation by multiplying it with an image derivative taken relative to the time-lag parameter. The resulting image perturbation is thus a mapping of the velocity error in image space. This approach is computationally efficient and simple to implement, and no further assumptions about smoothness and homogeneity of the velocity model and reflector geometry are needed. Synthetic examples demonstrate the successful application of our method to a complex velocity model.
hnRNP A2/B1 binds specifically to single stranded vertebrate telomeric repeat TTAGGG<sub>n</sub> (1992)

We have previously isolated a protein from mouse liver nuclei that specifically binds to single stranded (TTAGGG)n repeats. TTAGGG is the telomeric repeats of mammals and we therefore named the new protein single stranded telomere binding protein (sTBP). Further studies now identify sTBP as heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 on the basis of amino acid sequence determination and antibody reactivity. A2 and B1 form a major part of the protein component of hnRNP particles and are abundant nuclear proteins. Unexpectedly, A2/B1 has a high specificity for binding to the RNA equivalent of TTAGGG, UUAGGG, but under the same conditions does not appear to have a strong affinity for a number of other RNA species.
Diagnosis of scan-chains by use of a configurable signature register and error-correcting codes (2004)

In this paper a new diagnosis method for scan designs with many scan-paths based on error correcting linear block codes with N information bits and K control bits is proposed, where N is the number of scan-paths. The new approach can be implemented on a modified STUMPS-architecture. In diagnosis mode the test has K times to be repeated. In the K repetitions of the test the outputs of the scan-paths are connected to a con.gurable signatureregister (with disconnected feedback logic) according to the coefficients of the K syndrome equations of the code. By monitoring the one-dimensional output sequence of the configurable signature register the failing scan-cells in the different scan-paths can be identified with the resolution of the selected error correcting code. Since for the relevant codes, e.g.(shortened) Hamming codes, T-error correcting BCH-code, the ratio {K \over N} decreases very fast with an increasing number N the method is useful for a large number of scan-paths.
Liner-Deployed Inflow Control Devices ICD Production Results in MacKay River SAGD Wells (2018)

Abstract Ever since horizontal drilling became prominent decades ago, it has been the goal of oilfield operators to find an effective mechanism for controlling the toe-to-heel flux into the production liner to delay and/or reduce the inflow of unwanted fluids such as water, gas and steam, in order to maximize sweep efficiency and oil production/recovery. Inflow Control Devices (ICDs) are basically flow restrictors installed along the completion string to alter the pressure drawdown on the reservoir by choking back the high permeability/high mobility zones while allowing more influx from the lower permeability/lower mobility zones. In steam assisted gravity drainage (SAGD) production wells the primary goal is to operate at lower subcool while minimizing live steam production. The pace of ICD technology adoption has accelerated amongst the operators since its benefit was made prominent in the Surmont field (Stalder 2012). PetroChinaCanada (formerly known as Brion Energy) recognized the technical opportunity and commenced implementation of an ICD technology trial at its MacKay River asset. The technology selection, ICDs sizing and performace prediction was conducted in 2013 (Becerra et al). In 2014 two SAGD production wells, each located on different pads, were selected to evaluate if ICDs could have a beneficial impact on performance. The purpose of this paper is to present preliminary results of the two ICD producer wells which, as of November 2017, have shown superior performance to their non-ICD neighboring wells after about 6 months of production.
Lattice-Gas Quantum Computation (1998)

We present a quantum lattice-gas model for a quantum computer operating with continual wavefunction collapse; entanglement of the wavefunction occurs locally over small spatial regions between nearby qubits for only a short time period. The quantum lattice-gas is a noiseless method that directly models the lattice-gas particle dynamics at the mesoscopic scale. The system behaves like a viscous Navier–Stokes fluid. Numerical simulations indicate the viscosity of the quantum lattice-gas fluid is lower than its classical lattice-gas counterpart's.
Androgen-Regulated Expression of a Novel Member of the Aldo-Keto Reductase Superfamily in Regrowing Rat Prostate (2000)

The rat prostate is dependent on androgen for normal growth and differentiation. In addition, the organ undergoes rapid cell death upon withdrawal of androgen on castration, and the atrophied tissue is capable of regrowth after androgen replacement in adult animals. In our search for novel factor(s) that participate in this androgen-induced proliferation of adult rat prostate cells, we have generated a complementary DNA (cDNA) library enriched in cDNAs transiently up-regulated after androgen stimulation in castrated rat ventral prostate using a PCR-based subtractive hybridization technique. Sequence analysis of about one hundred clones in the library showed that approximately 70% of them are identical or closely related to genes of known function, the remaining ones showing no or very low similarity to any genes characterized previously. Among the former a new member of the rat aldo-keto reductase superfamily that is closely related to aflatoxin, B1 aldehyde reductase has been identified. The newly identified protein (androgen-inducible aldehyde reductase, AIAR) and rat aflatoxin B1 aldehyde reductase (AFAR) exhibit 80% amino acid sequence homology. The enzymatic activity toward 4-nitrobenzaldehyde of recombinant AIAR expressed in Escherichia coli was about 16% of that of rat AFAR. Northern blot analysis revealed AIAR expression in various adult rat tissues in addition to the ventral and dorsolateral prostates, which differs from the highly restricted expression of AFAR in the kidney and liver. The AIAR messenger RNA (mRNA) content of the ventral prostate was low in normal and castrated rats, transiently increased after androgen administration to castrated rats, attaining a peak 12–24 h after the treatment. Although the physiological substrate(s) of AIAR has not been identified, the current results suggest that AIAR expression is associated with some growth-related processes in regrowing rat prostate.
Thirty-month follow-up of sub-optimal responders to multiple treatments with ivermectin, in two onchocerciasis-endemic foci in Ghana (2004)

The pathogenesis of the sub-optimal response of Onchocerca volvulus to ivermectin was investigated in a 30-month follow-up of 28 individuals who, in a previous study, had been found to show a sub-optimal (N = 15) or adequate response (N = 13) to multiple treatments with the drug. Verbal informed consent was obtained before each subject was given a general clinical and ocular examination. Skin snips were taken from both iliac crests and both calves. Seventeen nodule carriers were hospitalized for nodulectomy. Adult worms were harvested, embryogrammes were constructed and all developmental stages were counted; degenerate, stretched microfilariae were noted separately. All the subjects were in good general health and all except one had received at least one additional treatment with ivermectin since the earlier study. A large proportion of the adult female worms in 10 out of the 11 sub-optimal responders who were nodule carriers were in full embryonic production but most of the stretched microfilariae they carried were degenerate. This picture is similar to that found in adult worms exposed to the first dose of ivermectin. In one subject who had no viable worms in his nodules, the existence of occult but actively reproductive worms was inferred from the high level of microfilaridermia observed less than 12 months after treatment. These observations confirm the existence of populations of adult female O. volvulus that respond poorly to repeated doses of ivermectin. The use of suramin in the treatment of the sub-optimal responders is discussed.