gebhart/patrepeval huggingface dataset:
Interphase Nuclei of Many Mammalian Cell Types Contain Deep, Dynamic, Tubular Membrane-bound Invaginations of the Nuclear Envelope (1997)

The nuclear envelope consists of a double-membraned extension of the rough endoplasmic reticulum. In this report we describe long, dynamic tubular channels, derived from the nuclear envelope, that extend deep into the nucleoplasm. These channels show cell-type specific morphologies ranging from single short stubs to multiple, complex, branched structures. Some channels transect the nucleus entirely, opening at two separate points on the nuclear surface, while others terminate at or close to nucleoli. These channels are distinct from other topological features of the nuclear envelope, such as lobes or folds. The channel wall consists of two membranes continuous with the nuclear envelope, studded with features indistinguishable from nuclear pore complexes, and decorated on the nucleoplasmic surface with lamins. The enclosed core is continuous with the cytoplasm, and the lumenal space between the membranes contains soluble ER-resident proteins (protein disulphide isomerase and glucose-6-phosphatase). Nuclear channels are also found in live cells labeled with the lipophilic dye DiOC6. Time-lapse imaging of DiOC6-labeled cells shows that the channels undergo changes in morphology and spatial distribution within the interphase nucleus on a timescale of minutes. The presence of a cytoplasmic core and nuclear pore complexes in the channel walls suggests a possible role for these structures in nucleo-cytoplasmic transport. The clear association of a subset of these structures with nucleoli would also be consistent with such a transport role.
A Study on the Procedure of Decision-Making for Entrance Architecture of Modern MRT Station in Taiwan (2004)

The purpose of this study is to establish a set of consistent guidelines for deciding entrance architecture of modern MRT (Mass Rapid Transit) stations in Taiwan, so that the details of construction, passenger circulation, and operation and maintenance procedures will be uniform throughout the entire MRT network. There exist the multi-criteria evaluation of subjectivities and externalities for the decision. To fulfill the interaction between subjectivities and externalities, we applied the appropriate ways, which included 1) the method of key-informant interview with decision-makers, professionals, experts and scholars to establish the multi-criteria, 2) the method of questionnaire and mathematical calculation to establish the weighting of each criterion, and finally applied 3) the method of multi-criteria evaluation with qualitative and quantitative data (MEQQD) for decision making. After the procedure of this study, it was found that ′site response′ was the most dominant criterion and the method of MEQQD could evaluate the alternatives to obtain the objective and appropriated consequence.
The distribution of sodium and potassium in amphibian embryos during early development (1973)

1. Intracellular and intercellular concentrations of sodium and potassium have been measured in pregastrular embryos of Xenopus laevis and Amblystoma mexicanum. Calcium and magnesium contents have also been determined.2. Between egg and gastrula stages of development the potassium concentration is near to 60 m-mole/l. embryo. Up to the blastula stage the sodium concentration is near to 50 m-mole/l. embryo; the amount of sodium in the embryo begins to fall just before gastrulation.3. Embryo calcium and magnesium concentrations show no significant variations prior to gastrulation. Average calcium concentrations for the different stages range from 5 to 8 m-mole/l. embryo; magnesium concentrations lie between 12.4 and 17 m-mole/l. embryo.4. The intercellular fluid contains 100 mM sodium and 1 mM potassium; the majority of the sodium is ionically active. As no net uptake of sodium or potassium occurs before gastrulation these cations must have been transferred from the cells to the cavity.5. At all developmental stages studied, the intracellular potassium concentration is close to 100 m-mole/l. cell water. Intracellular sodium falls steadily from 80 m-mole/l. cell water in eggs to 30 m-mole/l. cell water at the beginning of gastrulation.6. The intracellular sodium activity, measured with sodium sensitive intracellular micro-electrodes, is relatively constant between egg and blastula stages at about 14 mM. During each cell division cycle the intracellular sodium activity rises transiently by 2-3 mM.
Vapor feeding of liquid metal cathodes (1970)

Modified liquid metal cathode apparatus permits vapor feeding in the diffuse-spot pattern mode. Vapor-fed cathode has advantages over liquid-fed systems.
Extended Object Tracking with Random Hypersurface Models (2014)

The random hypersurface model (RHM) is introduced for estimating a shape approximation of an extended object in addition to its kinematic state. An RHM represents the spatial extent by means of randomly scaled versions of the shape boundary. In doing so, the shape parameters and the measurements are related via a measurement equation that serves as the basis for a Gaussian state estimator. Specific estimators are derived for elliptic and star-convex shapes.
A mutant alpha subunit of G12 potentiates the eicosanoid pathway and is highly oncogenic in NIH 3T3 cells. (1993)

The discovery of GTPase-inhibiting mutations in genes for alpha subunits of Gs or G(i2) in certain human endocrine tumors has raised the possibility that heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) might contribute to neoplastic disease. Expression of GTPase-deficient alpha s or alpha i2 polypeptides in rodent fibroblasts increases or decreases cAMP, respectively, and induces certain alterations in cell growth but only a few of the phenotypic changes associated with cellular transformation. In contrast, an analogous mutation in the alpha subunit of Gq, which activates phosphatidylinositol (PI)-specific phospholipase C, is fully oncogenic. However, activated alpha q is cytotoxic and several orders of magnitude less potent as an oncogene than certain G protein-coupled receptors. Thus, G proteins other than those inducing PI hydrolysis might possess high transforming efficiency. In the present study, we explored the G12 family of G proteins for their oncogenic potential. Our results show that whereas overexpression of wild-type alpha 12 in NIH 3T3 cells is itself weakly transforming, an activated alpha 12 behaves as a remarkably potent oncogene. Transformation by alpha 12 correlates with alterations in the eicosanoid pathway but not with PI-specific phospholipase C or other G protein-linked second messengers.
An Efficient One-Pot Method for the Large-Quantitative Production of Radiopharmaceutical Ligand: Diazadioximes. (2000)

An efficient one-pot procedure for the preparation of diazadioxime was described. Treatment of ketooximes with alkyldiamine followed by NaBH4 in dry ethanol afforded the corresponding d,l-diazadioximes in 56--74% yield without isolation of the intermediates.
Direct measurement of grating chirp using resonantsidescatter spectra (1996)

The local chirp parameter along a grating length is measured directly using the variation in resonant sidescatter spectral peaks as a function of grating length. The resonant sidescater spectra is suitable for characterising the variation in period along waveguide Bragg grating devices.
Assessment of the bradyphrenia of parkinsonism: a novel use of delayed auditory feedback (1993)

Sufferers from parkinsonism have difficulty shifting or developing an appropriate mental set. Delayed auditory feedback may, therefore, disrupt their speech more than that of healthy controls. This was the case when 104 subjects with idiopathic parkinsonism and 144 without were compared. Moreover, the disruptive effect was complementary, in discriminating between those with and without clinical parkinsonism, to the response in reaction time to a warning. Unlike the latter, the disruption caused by delayed auditory feedback appeared independent of mental test score results and uninfluenced by consumption of tobacco or anti-parkinsonian therapy. Neither were influenced by a rating of affect. More precise delineation of the mental disorders of parkinsonism is needed in clinical practice, set against background information on prognosis and drug responsiveness.
Additional New and Unreported Species of Mycorrhizal Fungi (Endogonaceae) from Florida (1982)

The occurrence of five species of Endogonaceae previously unreported from Florida is noted and seven new species (six in Glomus) are described. Glomus aggregatum is the only new species from Florida which forms sporocarps in and around roots and is similar in appearance to the previously described taxon G.fasciculatum. Glomus intraradices forms yellow-brown spores almost exclusively in the root. Glomus claroideum has hyaline to yellow spores which are smaller but similar in appearance to the previously described taxon G. darum. Glomus leptotichum has relatively large white spores with a thin wall bearing a faint reticulum. Spores of Glomus fecundisporum are gray-white, have two walls and are frequently borne in clusters. Glomus tortuosum has a sinuous hyphal mantle covering the spore. Gigaspora albida has generally smaller spores with slightly thicker walls but is similar in spore shape and color to the previously described taxon G. gigantea. A synoptic key to the 34 species of Endogonaceae described in Florida is included.
ChemInform Abstract: SYNTHESIS WITH TIN TEMPLATES: PREPARATION OF MACROCYCLIC TETRALACTONES (1982)

Abstract Dibutylzinnoxid (I) bildet mit Glykolen wie (II) cyclische Kondensate wie (III).
Moloney Murine Leukemia Virus Envelope Protein Subunits, gp70 and Pr15E, Form a Stable Disulfide-Linked Complex (1998)

The nature and stability of the interactions between the gp70 and Pr15E/p15E molecules of murine leukemia virus (MLV) have been disputed extensively. To resolve this controversy, we have performed quantitative biochemical analyses on gp70-Pr15E complexes formed after independent expression of the amphotropic and ecotropic Moloney MLV env genes in BHK-21 cells. We found that all cell-associated gp70 molecules are disulfide linked to Pr15E whereas only a small amount of free gp70 is released by the cells. The complexes were resistant to treatment with reducing agents in vivo, indicating that the presence and stability of the disulfide interaction between gp70 and Pr15E are not dependent on the cellular redox state. However, disulfide-bonded Env complexes were disrupted in lysates of nonalkylated cells in a time-, temperature-, and pH-dependent fashion. Disruption seemed not to be caused by a cellular factor but is probably due to a thiol-disulfide exchange reaction occurring within the Env complex after solubilization. The possibility that alkylating agents induce the formation of the intersubunit disulfide linkage was excluded by showing that disulfide-linked gp70-Pr15E complexes exist in freshly made lysates of nonalkylated cells and that disruption of the complexes can be prevented by lowering the pH. Together, these data establish that gp70 and Pr15E form a stable disulfide-linked complex in vivo.
Contra: The Commission and the Stability Pact (2004)

Wachstumspolitik, Reform, Politische Stabilität, EU-Recht, Growth policy, Political stability, Community law
On the beta-binomial model for analysis of spectral count data in label-free tandem mass spectrometry-based proteomics (2009)

Supplementary data are available at Bioinformatics online.
Secure, Dependable, and High Performance Cloud Storage (2010)

There have been works considering protocols for accessing partitioned data. Most of these works assume the local cluster based environment and their designs target atomic semantics. However, when considering widely distributed cloud storage systems, these existing protocols may not scale well. In this paper, we analyze the requirements of access protocols for storage systems based on data partitioning schemes in widely distributed cloud environments. We consider the regular semantics instead of atomic semantics to improve access efficiency. Then, we develop an access protocol following the requirements to achieve correct and efficient data accesses. Various protocols are compared experimentally and the results show that our protocol yields much better performance than the existing ones.
Identification of Peptides Derived from the Human Antimicrobial Peptide LL-37 Active against Biofilms Formed by Pseudomonas aeruginosa Using a Library of Truncated Fragments (2012)

Persistent Pseudomonas aeruginosa infections are a major cause of morbidity and mortality in cystic fibrosis (CF) patients and are linked to the formation of a biofilm. The development of new biofilm inhibition strategies is thus a major challenge. LL-37 is the only human antimicrobial peptide derived from cathelicidin. The effects on the P. aeruginosa PAO1 strain of synthetic truncated fragments of this peptide were compared with the effects of the original peptide. Fragments of LL-37 composed of 19 residues (LL-19, LL13-31, and LL7-25) inhibited biofilm formation. The strongest antibiofilm activity was observed with the peptides LL7-37 and LL-31, which decreased the percentage of biomass formation at a very low concentration. Some peptides were also active on the bacteria within an established biofilm. LL7-31, LL-31, and LL7-37 increased the uptake of propidium iodide (PI) by sessile bacteria. The peptide LL7-37 decreased the height of the biofilm and partly disrupted it. The peptides active within the biofilm had an infrared spectrum compatible with an α-helix. LL-37, but not the peptides LL7-31 and LL7-37, showed cellular toxicity by permeabilizing the eukaryotic plasma membrane (uptake of ethidium bromide and release of lactate dehydrogenase [LDH]). None of the tested peptides affected mitochondrial activity in eukaryotic cells. In conclusion, a 25-amino-acid peptide (LL7-31) displayed both strong antimicrobial and antibiofilm activities. The peptide was even active on cells within a preformed biofilm and had reduced toxicity toward eukaryotic cells. Our results also suggest the contribution of secondary structures (α-helix) to the activity of the peptides on biofilms.
Combination Chemotherapy in Rheumatoid Arthritis (1988)

Combination Chemotherapy in Rheumatoid Arthritis Get access MARGARET FARR, MARGARET FARR Department of Rheumatology, The Medical School, University of BirminghamBirmingham B15 2TJ,UK Search for other works by this author on: Oxford Academic PubMed Google Scholar O. D. KITAS, O. D. KITAS Department of Rheumatology, The Medical School, University of BirminghamBirmingham B15 2TJ,UK Search for other works by this author on: Oxford Academic PubMed Google Scholar P. A. BACON P. A. BACON Department of Rheumatology, The Medical School, University of BirminghamBirmingham B15 2TJ,UK Search for other works by this author on: Oxford Academic PubMed Google Scholar Rheumatology, Volume 27, Issue 1, February 1988, Pages 73–74, https://doi.org/10.1093/rheumatology/27.1.73 Published: 01 February 1988 Article history Received: 03 October 1987 Published: 01 February 1988
MicroRNAs-10a and -10b Contribute to Retinoic Acid-induced Differentiation of Neuroblastoma Cells and Target the Alternative Splicing Regulatory Factor SFRS1 (SF2/ASF) (2010)

MicroRNAs (miRNAs) are an emerging class of non-coding endogenous RNAs involved in multiple cellular processes, including cell differentiation. Treatment with retinoic acid (RA) results in neural differentiation of neuroblastoma cells. We wanted to elucidate whether miRNAs contribute to the gene expression changes induced by RA in neuroblastoma cells and whether miRNA regulation is involved in the transduction of the RA signal. We show here that RA treatment of SH-SY5Y neuroblastoma cells results in profound changes in the expression pattern of miRNAs. Up to 42 different miRNA species significantly changed their expression (26 up-regulated and 16 down-regulated). Among them, the closely related miR-10a and -10b showed the most prominent expression changes. Induction of miR-10a and -10b by RA also could be detected in LA-N-1 neuroblastoma cells. Loss of function experiments demonstrated that miR-10a and -10b are essential mediators of RA-induced neuroblastoma differentiation and of the associated changes in migration, invasion, and in vivo metastasis. In addition, we found that the SR-family splicing factor SFRS1 (SF2/ASF) is a target for miR-10a -and -10b in HeLa and SH-SY5Y neuroblastoma cells. We show here that changes in miR-10a and -10b expression levels may regulate SFRS1-dependent alternative splicing and translational functions. Taken together, our results give support to the idea that miRNA regulation plays a key role in RA-induced neuroblastoma cell differentiation. The discovery of SFRS1 as direct target of miR-10a and -10b supports the emerging functional interaction between two post-transcriptional mechanisms, microRNAs and splicing, in the neuronal differentiation context.
Mucosal mast cells. II. Effects of anti-allergic compounds on histamine secretion by isolated intestinal mast cells. (1982)

Functional mast cells have been isolated from the lamina propria of the small intestine of rats infected with the nematode Nippostrongylus brasiliensis. The cells released histamine on challenge with specific antigen, anti-rat IgE, concanavalin A, and calcium ionophores but were less responsive than peritoneal mast cells (MMC) from the same animals. Intestinal mucosa mast cells (PMC) were refractory to the action of the basic secretagogues peptide 401 from bee venom and compound 48/80. The anti-allergic compounds disodium cromoglycate (less than or equal to 10(-3) M), AH 9679 (less than or equal to 10(-4) M), and theophylline (less than or equal to 10(-2)) did not inhibit antigen-induced histamine secretion by MMC, although these compounds were effective against PMC. In contrast, doxantrazole (10(-5) to 10(-3) M) inhibited the secretion of histamine from both MMC and PMC in a comparable dose-dependent fashion. Thus, we have established that mast cells from different sites are functionally heterogeneous not only in their response to various stimuli for histamine secretion, but also in their responses to different pharmacologic modulators of secretion. It cannot be assumed that anti-allergic compounds effective against mast cells in one tissue site or organ will be equally efficacious against mast cells in other sites. The extent of this functional heterogeneity must be established, and its investigation may provide new insights into the biochemical events involved in mast cell secretion.
About Brezis-Merle Problem with Holderian condition (2014)

Equipe d’Analyse Complexe et Geometrie, Universite Paris VI, 75005, Paris, Farnce Abstract We consider the following problem on open set â¦ of R 2 : ( −âui = Vie ui in â¦ ⊂ R 2 , ui = 0 in ∂â¦. Under some conditions , we give a quantization analysis and a compactness result for the previous problem.
Milacemide: A Placebo‐Controlled Study in Senile Dementia of the Alzheimer Type (1992)

Milacemide does not appear to be an effective treatment in enhancing cognition in SDAT patients.
Path diversity reception using SMI steering vector arrays and multi-trellis Viterbi equalizer (2002)

In this paper, path diversity reception using sample matrix inversion (SMI) steering vector arrays and a multi-trellis Viterbi equalizer is proposed to cope with frequency-selective fading. The proposed scheme estimates the channel impulse response and selects the delayed path that provides maximum path power. It then calculates two sets of array weight and output response vectors to extract the direct and selected paths and to estimate the array output response with the single constraint SMI algorithm. The branch-metric-combining multi-trellis Viterbi algorithm is used to estimate the most likely transmitted sequence from the array output signals. Simulation results show that the proposed scheme is superior to the previously proposed scheme in frequency-selective fading channels.
Vehicle-Network Development based on a Communications-Network Testbed (2006)

Light armoured vehicles rely on sensors, on-board computing and digital wireless communications to achieve improved performance and survivability. Constrained by low latency response to threats, individual vehicles share sensory information with other platoon vehicles benefiting from a flexible, dynamic, self-adapting network environment. As sensor and computing capability increases, network communications become saturated. To understand the operational requirements for these future vehicle networks, the high capacity technical communications network (HCTCN) low bandwidth testbed (LBTB) has been developed to provide a simulated environment for the radios and candidate database and transmission protocols selected. These concepts and approach to network communications is discussed in the paper
Archaeal protein translocation (2000)

Proper cell function relies on correct protein localization. As a first step in the delivery of extracytoplasmic proteins to their ultimate destinations, the hydrophobic barrier presented by lipid-based membranes must be overcome. In contrast to the well-defined bacterial and eukaryotic protein translocation systems, little is known about how proteins cross the membranes of archaea, the third and most recently described domain of life. In bacteria and eukaryotes, protein translocation occurs at proteinaceous sites comprised of evolutionarily conserved core components acting in concert with other, domain-specific elements. Examination of available archaeal genomes as well as cloning of individual genes from other archaeal strains reveals the presence of homologues to selected elements of the bacterial or eukaryotic translocation machines. Archaeal genomic searches, however, also reveal an apparent absence of other, important components of these two systems. Archaeal translocation may therefore represent a hybrid of the bacterial and eukaryotic models yet may also rely on components or themes particular to this domain of life. Indeed, considering the unique chemical composition of the archaeal membrane as well as the extreme conditions in which archaea thrive, the involvement of archaeal-specific translocation elements could be expected. Thus, understanding archaeal protein translocation could reveal the universal nature of certain features of protein translocation which, in some cases, may not be readily obvious from current comparisons of bacterial and eukaryotic systems. Alternatively, elucidation of archaeal translocation could uncover facets of the translocation process either not yet identified in bacteria or eukaryotes, or which are unique to archaea. In the following, the current status of our understanding of protein translocation in archaea is reviewed.