gebhart/patrepeval huggingface dataset:
Conditional autoimmunity mediated by human natural anti‐FcεRIα autoantibodies? (2001)

Natural antibodies provide an early defense mechanism against pathogens, show a frequent self-reactivity, and are present throughout life. Two questions concern the physiological control of self-reactivity and the pathogenetic link to autoimmune disease. Here we propose a concept of conditional autoimmunity involving natural antibodies against the alpha chain of the high-affinity receptor for IgE (Fc(epsilon)RIalpha ). Like other natural antibodies, anti-Fc(epsilon)RIalpha antibodies are found in sera of healthy donors. We now report the first human recombinant anti-Fc(epsilon)RIalpha autoantibodies isolated by repertoire cloning from a human tonsillar IgM library. These high-affinity antibodies recognize Fc(epsilon)RIalpha on cells and trigger histamine release from freshly isolated blood basophils. However, the latter effect requires IgE removal from the Fc(epsilon)RI. The same conditional histamine release is seen when using sera from individual normal donors and affinity-purified anti-Fc(epsilon)RIalpha antibodies isolated from multidonor therapeutic IgG preparations. We propose that such anti-Fc(epsilon)RIalpha antibodies can become pathogenic and that this is dependent on the state of occupancy of the Fc(epsilon)RIalpha by its natural ligand IgE. We suggest that an imbalance between Fc(epsilon)RIalpha occupancy and natural anti-Fc(epsilon)RIalpha antibodies may be implicated in the pathogenesis of autoimmune urticaria.
Energy aware Networks: Reducing Power Consumption by Switching Off Network Portions (2008)

According to several studies, the power consumption of the Internet accounts for up to 10% of the worldwide energy consumption, and several initiatives are being put into place to reduce the power consumption of the ICT sector in general. To this goal, we propose a novel approach to switch off network nodes and links while still guaranteeing full connectivity and maximum link utilization. After showing that the problem falls in the class of capacitated multi-commodity flow problems, and therefore it is NP-complete, we propose some heuristic algorithms to solve it. Results shows that it is possible to reduce the number of links and nodes currently used by up to 25% and 10% respectively while offering the same service quality.
Phosphonium labeling for increasing metabolomic coverage of neutral lipids using electrospray ionization mass spectrometry (2009)

Mass spectrometry has become an indispensable tool for the global study of metabolites (metabolomics), primarily using electrospray ionization mass spectrometry (ESI-MS). However, many important classes of molecules such as neutral lipids do not ionize well by ESI and go undetected. Chemical derivatization of metabolites can enhance ionization for increased sensitivity and metabolomic coverage. Here we describe the use of tris(2,4,6,-trimethoxyphenyl)phosphonium acetic acid (TMPP-AA) to improve liquid chromatography (LC)/ESI-MS detection of hydroxylated metabolites (i.e. lipids) from serum extracts. Cholesterol which is not normally detected from serum using ESI is observed with attomole sensitivity. This approach was applied to identify four endogenous lipids (hexadecanoyl-sn-glycerol, dihydrotachysterol, octadecanol, and alpha-tocopherol) from human serum. Overall, this approach extends the types of metabolites which can be detected using standard ESI-MS instrumentation and demonstrates the potential for targeted metabolomics analysis.
TopicFlow Model: Unsupervised Learning of Topic-specific Influences of Hyperlinked Documents (2011)

Modeling influence of entities in networked data is an important problem in information retrieval and data mining. Popular algorithms such as PageRank capture this notion of authority by analyzing the hyperlink structure, but they ignore the topical content of the document. However, often times, authority is topic dependent, e.g., a web page of high authority in politics may be an unknown entity in sports. In this work, we describe a new model called TopicFlow, that combines ideas from network flow and topic modeling, and captures the notion of topic specific influences of hyperlinked documents in a completely unsupervised fashion. We show that on the task of citation recommendation, the TopicFlow model, when combined with TF-IDF based cosine similarity, outperforms several competitive baselines by as much as 6.4%. We also present some qualitative visualizations to demonstrate the expressive power of the new model. 1
ANALYSIS OF <i>nm23</i> GENE EXPRESSIONS IN HUMAN BLADDER AND RENAL CANCERS (1994)

We measured nm23-H1 and nm23-H2 mRNA levels in tissues from 22 human bladder cancers and 16 renal cell carcinomas, and in 7 bladder cancer and 6 renal cancer cell lines by Northern blot and slot blot hybridization analyses. Differences in mRNA levels were evaluated in primary tumor tissues and in paired normal tissues and cell lines. Moreover, nm23 gene expression in primary tumor tissues was compared with clinicopathological features. High nm23-H1 and nm23-H2 expression was observed in cancerous areas of human bladder tissue (nm23-H1: p = 0.001, nm23-H2: p = 0.001) and bladder cancer cell lines (nm23-H1: p = 0.001, nm23-H2: p < 0.001) compared with that in normal bladder mucosa. However, mRNA levels of both nm23 genes were not associated with histological grade, pathological stage, tumor metastasis or prognosis. On the other hand, in human renal cell carcinomas, levels of both nm23 mRNAs in tumor tissues were similar to those in paired normal kidneys, but elevated in cultured cell lines (nm23-H1: p = 0.002, nm23-H2: p = 0.014). Moreover, there was a tendency towards high nm23 gene expression in grade 2 tumors compared with grade 1 (grade 1 vs grade 2, nm23-H1: p = 0.107, nm23-H2: p = 0.008; no grade 3 tumors in this study) and in high stage renal cancers (< or = stage II vs stage III < or =, nm23-H1: p = 0.023, nm23-H2: p = 0.005).(ABSTRACT TRUNCATED AT 250 WORDS)
Perinatal events in the development of asthma (1999)

Many potential factors are likely involved in the pathogenesis of asthma. These include prenatal, peripartum, and postnatal influences. Prenatally, genetic endowment, maternal smoking, in utero allergen sensitization, and alterations in maternal immune function, especially at the placental level, may increase the risk for asthma and atopy. In the peripartum period, suspected factors include obstetric practices (eg, the use of prostaglandins, hormones, and other agents) and prematurity. Postnatally passive smoke exposure, neonatal or early childhood infections and breast-feeding are under increasing scrutiny as to their possible role in the development of asthma. Despite the volumes of work already reported, much more is left to be done to sort out the complex interrelationships of these and other as yet unsuspected influences on the development of asthma.
Technical Aspects of Bilirubin Determination in Whole Blood (2005)

Spectroscopic analysis of hemoglobin (Hb) and its derivatives (CO oximetry), in combination with blood gas analysis, provides valuable information about oxygen transport in human blood. In neonatal care, there is additional interest in monitoring bilirubin due to its potentially brain-damaging effect. The device presented herein permits the simultaneous determination of Hb derivatives and bilirubin from a single sample as small as 60 μL, thus eliminating the need for excessive blood collection from the newborn.
Arachidonic acid production by microorganisms (1992)

Arachidonic acid (AA) is a natural precursor of prostaglandins, thromboxanes, leukotrienes, prostacyclins, and a large group of C20 compounds which are of intrinsic medical interest. At present, the only source of AA is animal tissues. However, it is present in several microorganisms. This review describes the production of AA by microorganisms and its recovery and the potential of microorganisms as a source of AA.
Applications for Navy Unmanned Aircraft Systems (2010)

Abstract : There has been tremendous growth in demand for unmanned aircraft systems (UASs) by the United States military since 2001. The United States Navy is making large investments in a number of major UAS programs, including Broad Area Maritime Surveillance (BAMS) UAS, the Unmanned Combat Aircraft System Demonstrator (UCAS-D), the Fire Scout vertical takeoff/landing tactical UAS (VTUAS), and the Small Tactical/Tier II UAS (STUAS/Tier II UAS). Navy OPNAV N81 asked RAND to provide an evaluation of the Navy's ongoing and proposed UAS programs and to describe the most promising applications of UAS to operational tasks. These assessments were to include arguments for and against using manned vehicles to perform the same tasks as unmanned vehicles, where appropriate. Completed in September 2008, the study does not provide an exhaustive look at all DoD missions for UAS. It does, however, discuss the strengths and weaknesses of manned and unmanned aircraft for certain missions of importance to the Navy. We emphasized traditional Navy missions rather than Navy contributions to irregular warfare, though we examined a few nontraditional missions, such as counter-piracy.
ABSTRACT: Process-based Stochastic Modeling of Meandering Channelized Reservoirs (2001)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Hybrid predictive control strategy for a public transport system with uncertain demand (2010)

In this article, a hybrid predictive control (HPC) strategy is formulated for the real-time optimisation of a public transport system operation run using buses. For this problem, the hybrid predictive controller corresponds to the bus dispatcher, who dynamically provides the optimal control actions to the bus system to minimise users’ total travel time (on-vehicle ride time plus waiting time at stops). The HPC framework includes a dynamic objective function and a predictive model of the bus system, written in discrete time, where events are triggered when a bus arrives at a bus stop. Upon these events, the HPC controller makes decisions based on two well-known real-time transit control actions, holding and expressing. Additionally, the uncertain passenger demand is included in the model as a disturbance and then predicted based on both offline and online information of passenger behaviour. The resulting optimisation problem of the HPC strategy at every event is Np-hard and needs an efficient algorithm to solve it in terms of computation time and accuracy. We chose an ad hoc implementation of a Genetic Algorithm that permits the proper management of the trade-off between these two aspects. For real-time implementation, the design of this HPC strategy considers newly available transport technology such as the availability of automatic passenger counters (APCs) and automatic vehicle location (AVL) devices. Illustrative simulations at 2, 5 and 10 steps ahead are conducted, and promising results showing the advantages of the real-time control schemes are reported and discussed.
Mapping the Binding Site for Matrix Metalloproteinase on the N-Terminal Domain of the Tissue Inhibitor of Metalloproteinases-2 by NMR Chemical Shift Perturbation (1997)

Changes in the NMR chemical shift of backbone amide nuclei (1H and 15N) have been used to map the matrix metalloproteinase (MMP) binding site on the N-terminal domain of the tissue inhibitor of metalloproteinase-2 (N-TIMP-2). Amide chemical shift changes were measured on formation of a stable complex with the catalytic domain of stromelysin-1 (N-MMP-3). Residues with significantly shifted amide signals mapped specifically to a broad site covering one face of the molecule. This site (the MMP binding site) consists primarily of residues 1-11, 27-41, 68-73, 87-90, and 97-104. The site overlaps with the OB-fold binding site seen in other proteins that share the same five-stranded beta-barrel topology. Sequence conservation data and recent site-directed mutagenesis studies are discussed in relation to the MMP binding site identified in this work.
Roseobacter algicola sp. nov., a New Marine Bacterium Isolated from the Phycosphere of the Toxin-Producing Dinoflagellate Prorocentrum lima (1995)

We describe a new species on the basis of phenotypic characteristics and the results of an analysis of small-subunit rRNA sequences. Three strains of this organism were isolated from a culture of the toxin-producing dinoflagellate Prorocentrum lima. These bacteria are gram-negative, strictly aerobic, ovoid organisms that are motile by means of one or two subpolar flagella. They grow at temperatures ranging from 10 to 37 degrees C and in the presence of NaCl concentrations ranging from 0.1 to 2 M and have an absolute requirement for sodium ions. They are strictly aerobic with a nonfermentative type of metabolism and are not able to grow anaerobically in presence or absence of nitrate. They do not denitrify. They exhibit oxidase, catalase, gelatinase, esculinase, beta-galactosidase, and (to a lesser extent) amylase activities. The three strains which we examined require thiamine and biotin for growth. They grow only when glucose, trehalose, saccharose, fructose, maltose, pyruvate, malate, citrate, esculin, 2-ketoglutarate, 5-ketogluconate, glutamate, or shikimate is present as a sole carbon source. The three strains have identical small-subunit rRNA sequences. A phylogenetic analysis of these sequences revealed that these bacteria belong to the alpha subdivision of the Proteobacteria and that they form a distinct and robust monophyletic group with Roseobacter denitrificans and Roseobacter litoralis. This result and the general phenotypic characteristics of the organisms place them in the genus Roseobacter, although they do not produce bacteriochlorophyll a, in contrast to previously described Roseobacter species.(ABSTRACT TRUNCATED AT 250 WORDS)
Intellectual Property Rights in the Internet Era: the New Frontier, 5 J. Marshall Rev. Intell. Prop. L. 589 (2006) (2006)

With the rapid development of information and Internet technologies, how to protect intellectual property (IP) rights in the Internet era became a new frontier for IP practitioners and scholars internationally. This article highlights some important IP protection issues related to copyright, patent, trademark, and domain names, as well as the impact of technological advances on IP protection in the Internet era. The author believes that in order to solve these new emerging issues, the most important principle is keeping the balance between different sides with stakes in the IP right. Finally, international cooperation must be enhanced with more technical assistance to be offered by the developed countries to the developing countries in order to make the world more harmonious and balanced in terms of IP protection in this Internet era.
Heterogeneity in Formation of Lignin. VII. An Autoradiographic Study on the Formation of Guaiacyl and Syringyl Lignin in Poplar (1986)

Abstract Precursors of lignin biosynthesis, ferulic acid and sinapic acid labeled by 3H or 14C were administered to the differentiating xylem of poplar shoots. Biochemical interconversions between syringyl and guaiacyl type intermediates involved in the biosynthetic pathway were suppressed by administration of precursors in the dark. The process of heterogeneous deposition of guaiacyl and syringyl lignin on the cell wall was visualized by autoradiography. Guaiacyl lignin was deposited in the early stage of the xylem differentiation on the vessel wall followed by deposition of syringyl lignin on the fiber cell wall.
The human H5N1 influenza A virus polymerase complex is active in vitro over a broad range of temperatures, in contrast to the WSN complex, and this property can be attributed to the PB2 subunit (2008)

Influenza A virus (IAV) replicates in the upper respiratory tract of humans at 33 degrees C and in the intestinal tract of birds at close to 41 degrees C. The viral RNA polymerase complex comprises three subunits (PA, PB1 and PB2) and plays an important role in host adaptation. We therefore developed an in vitro system to examine the temperature sensitivity of IAV RNA polymerase complexes from different origins. Complexes were prepared from human lung epithelial cells (A549) using a novel adenoviral expression system. Affinity-purified complexes were generated that contained either all three subunits (PA/PB1/PB2) from the A/Viet/1203/04 H5N1 virus (H/H/H) or the A/WSN/33 H1N1 strain (W/W/W). We also prepared chimeric complexes in which the PB2 subunit was exchanged (H/H/W, W/W/H) or substituted with an avian PB2 from the A/chicken/Nanchang/3-120/01 H3N2 strain (W/W/N). All complexes were functional in transcription, cap-binding and endonucleolytic activity. Complexes containing the H5N1 or Nanchang PB2 protein retained transcriptional activity over a broad temperature range (30-42 degrees C). In contrast, complexes containing the WSN PB2 protein lost activity at elevated temperatures (39 degrees C or higher). The E627K mutation in the avian PB2 was not required for this effect. Finally, the avian PB2 subunit was shown to confer enhanced stability to the WSN 3P complex. These results show that PB2 plays an important role in regulating the temperature optimum for IAV RNA polymerase activity, possibly due to effects on the functional stability of the 3P complex.
Activation of Epithelial Sodium Channels by Prostasin in Xenopus Oocytes (2001)

Prostasin, a novel serine protease, was purified from seminal fluid, and its cDNA sequence was determined. Expression of prostasin was detected in human tissues, including prostate, kidney, and lung, as well as bodily fluids, including seminal fluid and urine. However, its physiologic role in the human body is not known. Recently, a novel regulatory mechanism by which serine proteases activate epithelial sodium channel in the Xenopus oocyte was identified. Therefore, it was hypothesized that prostasin could activate sodium currents, and a rat prostasin cDNA clone was isolated to investigate its physiologic function. Rat prostasin mRNA is expressed predominantly in kidney, and lower levels of expression were detected in prostate, lung, colon, stomach, and skin. These all are epithelial tissues in which the epithelial sodium channel (ENaC) is expressed. Coexpression of rat prostasin and rat ENaC in Xenopus oocytes increased the amiloride-sensitive sodium current by twofold. Preincubation of oocytes that expressed prostasin with aprotinin did not result in an increase in sodium current, compared with the control. The removal of aprotinin from the bath solution resulted in a twofold increase of the current only in oocytes that expressed prostasin, which indicates that protease activity of prostasin is required for the ENaC activation. Expression of rat prostasin had no effect on the potassium current when expressed with rat renal outer medulla K channel, which shows specificity of prostasin action for ENAC: These results indicate that prostasin acts as an extracellular regulator of ENAC:
Symbiotic jobscheduling for a simultaneous mutlithreading processor (2000)

Simultaneous Multithreading machines fetch and execute instructions from multiple instruction streams to increase system utilization and speedup the execution of jobs. When there are more jobs in the system than there is hardware to support simultaneous execution, the operating system scheduler must choose the set of jobs to coscheduleThis paper demonstrates that performance on a hardware multithreaded processor is sensitive to the set of jobs that are coscheduled by the operating system jobscheduler. Thus, the full benefits of SMT hardware can only be achieved if the scheduler is aware of thread interactions. Here, a mechanism is presented that allows the scheduler to significantly raise the performance of SMT architectures. This is done without any advance knowledge of a workload's characteristics, using sampling to identify jobs which run well together.We demonstrate an SMT jobscheduler called SOS. SOS combines an overhead-free sample phase which collects information about various possible schedules, and a symbiosis phase which uses that information to predict which schedule will provide the best performance. We show that a small sample of the possible schedules is sufficient to identify a good schedule quickly. On a system with random job arrivals and departures, response time is improved as much as 17% over a schedule which does not incorporate symbiosis.
Prodiginine (prodigiosin-like) pigments from <i>Streptomyces</i> and other aerobic Actinomycetes (1976)

About 95 microorganisms, mainly streptomycetes, were examined for prodiginine pigments. The test methods, including some media for stimulating pigment production, are given. All nine strains which produced prodiginine pigments were characterized and the pigments identified. The results were used to propose structures for five prodigiosin-like pigments from actinomycetes reported in the literature between 1947 and 1964. Streptoverticillium sp. 26-1 furnished good yields of butylcycloheptylprodiginine (I), whose antimicrobial activity is given.
The Effect of Ambulance Response Time on Survival Following Out-of-Hospital Cardiac Arrest (2018)

Rapid ambulance response is associated with a higher rate of survival from OHCA with good neurological outcome. The response time, independently of whether bystander resuscitation measures are provided, ha^ a significant independent effect on the survival rate. In drawing conclusions from these findings, one should bear in mind that this was a retrospective registry study, with the corresponding limitations.
3D MICRO X-RAY FLUORESCENCE ANALYSIS (2006)

This thesis introduces a new setup which extends the capabilities of X-ray spectroscopy. A confocal alignment of two polycapillary X-ray lenses facilitates three dimensional resolved X-ray fluorescence analysis (3D micro-XRF). With a spatial resolution of down to around 10 µm to 20 µm the determination of elemental densities is feasible nondestructively. Even information on the chemical state of elements can be obtained by exploring the X-ray Absorption Fine Structure (3D micro-XAFS) of elements of interest.The two modes, XRF and XAFS, of this new 3D analysis, their already successful application and their prospects as well as their restrictions and problems are portrayed with a detailed example of application for each one. We investigated paint layers and under glaze paintings, historical artifacts both of them, in order to throw light on the process of their production.Most of these experiments were performed with a synchrotron radiation source, but we also built up a 3D micro-XRF experiment with an X-ray tube. So far, 3D micro-XRF and 3D micro-XAFS prooved to be capable of providing valuable analytical results. Semiquantitative evaluations of depth profiling measurements were performed and the developments leading beyond that give hope that an entirely quantitative evaluation may be within reach soon.