gebhart/patrepeval huggingface dataset:
Effects and Action Mechanisms of Berberine and Rhizoma coptidis on Gut Microbes and Obesity in High-Fat Diet-Fed C57BL/6J Mice (2011)

Gut microbes play important roles in regulating fat storage and metabolism. Rhizoma coptidis (RC) and its main active compound, berberine, have either antimicrobial or anti-obesity activities. In the present study, we hypothesize that RC exerts anti-obesity effects that are likely mediated by mechanisms of regulating gut microbes and berberine may be a key compound of RC. Gut microbes and glucose and lipid metabolism in high-fat diet-fed C57BL/6J (HFD) mice in vivo are investigated after RC and berberine treatments. The results show that RC (200 mg/kg) and berberine (200 mg/kg) significantly lower both body and visceral adipose weights, and reduce blood glucose and lipid levels, and decrease degradation of dietary polysaccharides in HFD mice. Both RC and berberine significantly reduce the proportions of fecal Firmicutes and Bacteroidetes to total bacteria in HFD mice. In the trial ex vivo, both RC and berberine significantly inhibit the growth of gut bacteria under aerobic and anaerobic conditions. In in vitro trials, both RC and berberine significantly inhibit the growth of Lactobacillus (a classical type of Firmicutes) under anaerobic conditions. Furthermore, both RC and berberine significantly increase fasting-induced adipose factor (Fiaf, a key protein negatively regulated by intestinal microbes) expressions in either intestinal or visceral adipose tissues. Both RC and berberine significantly increase mRNA expressions of AMPK, PGC1α, UCP2, CPT1α, and Hadhb related to mitochondrial energy metabolism, which may be driven by increased Fiaf expression. These results firstly suggest that antimicrobial activities of RC and berberine may result in decreasing degradation of dietary polysaccharides, lowering potential calorie intake, and then systemically activating Fiaf protein and related gene expressions of mitochondrial energy metabolism in visceral adipose tissues. Taken together, these action mechanisms may contribute to significant anti-obesity effects. Findings in the present study also indicate that pharmacological regulation on gut microbes can develop an anti-obesity strategy.
Structural Analysis of DFG‐in and DFG‐out Dual Src‐Abl Inhibitors Sharing a Common Vinyl Purine Template (2009)

Bcr-Abl is the oncogenic protein tyrosine kinase responsible for chronic myeloid leukemia (CML). Treatment of the disease with imatinib (Gleevec) often results in drug resistance via kinase mutations at the advanced phases of the disease, which has necessitated the development of new mutation-resistant inhibitors, notably against the T315I gatekeeper mutation. As part of our efforts to discover such mutation resistant Abl inhibitors, we have focused on optimizing purine template kinase inhibitors, leading to the discovery of potent DFG-in and DFG-out series of Abl inhibitors that are also potent Src inhibitors. Here we present crystal structures of Abl bound by two such inhibitors, based on a common N9-arenyl purine, and that represent both DFG-in and -out binding modes. In each structure the purine template is bound deeply in the adenine pocket and the novel vinyl linker forms a non-classical hydrogen bond to the gatekeeper residue, Thr315. Specific template substitutions promote either a DFG-in or -out binding mode, with the kinase binding site adjusting to optimize molecular recognition. Bcr-Abl T315I mutant kinase is resistant to all currently marketed Abl inhibitors, and is the focus of intense drug discovery efforts. Notably, our DFG-out inhibitor, AP24163, exhibits modest activity against this mutant, illustrating that this kinase mutant can be inhibited by DFG-out class inhibitors. Furthermore our DFG-out inhibitor exhibits dual Src-Abl activity, absent from the prototypical DFG-out inhibitor, imatinib as well as its analog, nilotinib. The data presented here provides structural guidance for the further design of novel potent DFG-out class inhibitors against Src, Abl and Abl T315I mutant kinases.
Erythropoietin Increases Neurogenesis and Oligodendrogliosis of Subventricular Zone Precursor Cells After Neonatal Stroke (2013)

These data suggest that subventricular zone neural progenitor cells proliferate and migrate to the site of injury after neonatal stroke and multiple doses of EPO, with a shift in cell fate toward neurogenesis and oligodendrogliosis at both early and late time points. The contribution of local cell proliferation and neurogenesis remains to be determined.
Tourniquets: A Review of Current Use with Proposals for Expanded Prehospital Use (2008)

The use of arterial tourniquets in prehospital emergency care has been fraught with controversy and superstition for many years despite the potential utility of these tools. This review examines this controversy in the context of the history of the tourniquet as well as its recent use in surgery and modern battlefield casualty care. Safe prehospital tourniquet use is widespread in the military and is based on sound physiologic data and clinical experience from the surgical use of tourniquets. The physiologic, pathophysiologic, and clinical underpinnings of safe tourniquet use are reviewed here, along with a discussion of alternatives to tourniquets. Prehospital settings in which tourniquets are useful include tactical emergency medical services (EMS) and other law enforcement environments as well as disaster and mass casualty incidents. Beyond this, we present arguments for tourniquet use in more routine EMS settings, in which it may be beneficial but has heretofore been considered inappropriate. Protocols that foster safe, effective prehospital tourniquet use in these settings are then presented. Finally, we discuss future directions in which tourniquet research and other initiatives will further enhance the safe, rational use of this potentially life-saving tool.
Structure and Cytopathic Effects of Nelson Bay Virus (1970)

A virus isolated from a flying fox (Pteropus poliocephalus) has a morphology similar to that of reoviruses. However, unlike the reoviruses, this virus has a rapid cytopathic effect, causing cell fusion, vacuolization of the cytoplasm, and an unusual nuclear degeneration. Immunofluorescence indicates that viral antigen is distributed through both nucleus and cytoplasm. Viral maturation appears to take place in discrete cytoplasmic factories, and there is no apparent involvement of spindle tubules.
Antitumor Activity of the Investigational Proteasome Inhibitor MLN9708 in Mouse Models of B-cell and Plasma Cell Malignancies (2011)

Our results clearly showed the antitumor activity of MLN2238 in a variety of mouse models of B-cell lymphoma and PCM, supporting its clinical development. MLN9708 is being evaluated in multiple phase I and I/II trials.
Myeloid thrombomodulin lectin-like domain inhibits osteoclastogenesis and inflammatory bone loss (2016)

Osteoclastogenesis is an essential process during bone metabolism which can also be promoted by inflammatory signals. Thrombomodulin (TM), a transmembrane glycoprotein, exerts anti-inflammatory activities such as neutralization of proinflammatory high-mobility group box 1 (HMGB1) through TM lectin-like domain. This study aimed to identify the role of myeloid TM (i.e., endogenous TM expression on the myeloid lineage) in osteoclastogenesis and inflammatory bone loss. Using human peripheral blood mononuclear cells and mouse bone marrow-derived macrophages, we observed that the protein levels of TM were dramatically reduced as these cells differentiated into osteoclasts. In addition, osteoclastogenesis and extracellular HMGB1 accumulation were enhanced in primary cultured monocytes from myeloid-specific TM-deficient mice (LysMcre/TM(flox/flox)) and from TM lectin-like domain deleted mice (TM(LeD/LeD)) compared with their respective controls. Micro-computerized tomography scans showed that ovariectomy-induced bone loss was more pronounced in TM(LeD/LeD) mice compared with controls. Finally, the inhibiting effects of recombinant TM lectin-like domain (rTMD1) on bone resorption in vitro, and bone loss in both the ovariectomized model and collagen antibody-induced arthritis model has been detected. These findings suggested that the myeloid TM lectin-like domain may inhibit osteoclastogenesis by reducing HMGB1 signaling, and rTMD1 may hold therapeutic potential for inflammatory bone loss.
Performance and reliability of InGaAsP superluminescent diode (1992)

The performance and reliability of a 1.3- mu m superluminescent diode (SLD) with a novel structure are reported. A window structure with a tapered active layer is applied to suppress lasing oscillation. A V-groove structure is introduced to achieve high coupling efficiency into a single-mode fiber. The design of optimized device dimensions allows SLD operation to be obtained even at 0 degrees C. 1.3 mW is coupled into a single-mode fiber at 150 mA and 25 degrees C. The spectral modulation depth is 15% over the entire emission spectral width of 32 nm. The operating life of a SLD has been estimated from the results of accelerated aging carried out for 5000 h at the ambient temperatures of 50 and 125 degrees C. The activation energy of degradation is estimated to be 0.58 eV, and the extrapolated life is 10/sup 6/ h at an ambient temperature of 25 degrees C.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The <i>Arabidopsis PBS1</i> resistance gene encodes a member of a novel protein kinase subfamily (2001)

Specific recognition of Pseudomonas syringae strains that express the avirulence gene avrPphB requires two genes in Arabidopsis, RPS5 and PBS1. Previous work has shown that RPS5 encodes a member of the nucleotide binding site-leucine rich repeat class of plant disease resistance genes. Here we report that PBS1 encodes a putative serine-threonine kinase. Southern blot analysis revealed that the pbs1-1 allele contained a deletion of the 3' end of the PBS1 open reading frame. DNA sequence analysis of the pbs1-2 allele showed it to be a missense mutation that caused a glycine to arginine substitution in the activation segment of PBS1, a region known to regulate substrate binding and catalytic activity in many protein kinases. The identity of PBS1 was confirmed using both transient transformation and stable transformation of mutant pbs1 plants. Comparison of the predicted PBS1 amino acid sequence with other plant protein kinases revealed that PBS1 belongs to a distinct subfamily of protein kinases that contains no other members of known function. The Pto kinase of tomato, which is required for specific resistance to P. syringae strains expressing avrPto, did not fall in the same subfamily as PBS1 and is only 42% identical in the kinase domain. These data suggest that PBS1 and Pto may fulfil different functions in the recognition of pathogen avirulence proteins. We discuss several possible models for the roles of PBS1 and RPS5 in AvrPphB recognition.
Progression of Vertebral Wedging in an Asymmetrically Loaded Rat Tail Model (1997)

This study shows that vertebrae, when asymmetrically loaded, become wedged. This is consistent with the concept of mechanically provoked progression of scoliotic deformities according to the Hueter-Volkmann law.
Video Simulation for Training Land Design and Management. TRADOC Installations, Fiscal Years 1992-1993. (1995)

Technical Report
Efficient viscous design of realistic aircraft configurations (1998)

This paper addresses the use of the Constrained Direct Iterative Surface Curvature (CDISC) design method in the aircraft design process. A discussion of some of the requirements for practical use of CFD in the design process is followed by a description of different CFD design methods, along with their relative strengths and weaknesses. A detailed description of the CDISC design method highlights some of the aspects of the method that provide computational efficiency and portability, as well as the flow and geometry constraint capabilities. In addition, an efficient approach to multipoint design, the Weighted Averaging of Geometries (WAG) method, is described and illustrated using a couple of simple examples. The CDISC and WAG methods are then applied to a complex generic business jet geometry using an unstructured grid flow solver to demonstrate the multipoint and multicomponent design capabilities of these methods. Introduction
Spin-polarized current induced magnetization switch: Is the modulus of the magnetic layer conserved? (invited) (2002)

The direct effect of spin-polarized current on magnetization states is studied on various electrodeposited single contacted nanowires (diameter about 60 nm). Three kinds of samples have been studied: (1) Homogeneous Ni nanowires, (2) nanowires composed of both a homogeneous Ni part and a multilayered Co(10 nm)/Cu(10 nm) part, (3) pseudospin-valve pillars Co(30 nm)/Cu(10 nm)/Co(10) electrodeposited in Cu wires. The magnetization reversal due to the current injection is observed in the three cases. The effect is observed with using different experimental protocols, including current activated after-effect measurements. The results obtained suggest that two different mechanisms are able to account for the magnetization reversal: exchange torque and spin transfer. We propose a definition of the two mechanisms based on the conservation or nonconservation of the magnetic moment of the ferromagnetic nanostructure.
Sodium butyrate increases glucose transporter expression in LLC-PK1 cells. (1988)

The effect of sodium butyrate on the expression of the facilitated glucose transporter (GT) was investigated in the pig kidney cell line LLC-PK1. When cells were treated with butyrate, GT mRNA expression was remarkably enhanced with a maximal effect at 5 mM. Levels of GT mRNA were increased at 1 day after butyrate treatment and continued to increase for at least 4 days; however, acetate and propionate did not affect GT mRNA levels significantly. The induction of GT mRNA by butyrate was accompanied by an increase in GT function. The expression of GT mRNA decreased in HepG2, HT-29, and COS cells by treatment with butyrate for 1 day. Interestingly, glucose deprivation of LLC-PK1 cells reduced the induction of GT mRNA by butyrate, although starvation itself slightly enhanced steady-state GT mRNA levels. Therefore, expression of GT in LLC-PK1 cells is strongly induced by butyrate by a pathway that apparently depends on the presence of glucose in culture medium.
A wireless PDA-based physiological monitoring system for patient transport (2004)

This paper proposes a mobile patient monitoring system, which integrates current personal digital assistant (PDA) technology and wireless local area network (WLAN) technology. At the patient's location, a wireless PDA-based monitor is used to acquire continuously the patient's vital signs, including heart rate, three-lead electrocardiography, and SpO2. Through the WLAN, the patient's biosignals can be transmitted in real-time to a remote central management unit, and authorized medical staffs can access the data and the case history of the patient, either by the central management unit or the wireless devices. A prototype of this system has been developed and implemented. The system has been evaluated by technical verification, clinical test, and user survey. The evaluation of performance yields a high degree of satisfaction (mean = 4.64, standard deviation--SD = 0.53 in a five-point Likert scale) of users who used the PDA-based system for intrahospital transport. The results also show that the wireless PDA model is superior to the currently used monitors both in mobility and in usability, and is, therefore, better suited to patient transport.
Relationship between cytotoxic activity and dipole moment for phthalimido- and chloroethyl-phenothiazines. (2000)

Among twelve phenothiazine-related compounds, the cytotoxic activity of six "half-mustard type" phenothiazines [7-12] was significantly higher than that of six phthalimido compounds [1-6]. 1-(2-Chloroethyl)-3-(2-chloro-10H-phenothiazin-10-yl)propylurea [9], 1-(2-chloroethyl)-3-(2-chloro-10H-phenothiazin-10-yl)butylurea+ ++ [10] and 1-(2-chloroethyl)-3-(2-trifluoromethyl-10H-phenothiazin-10-yl)b utylurea [12] showed the highest cytotoxic activity, in parallel with high delta mu (difference between two dipole moments, mu g and mu e). There was also positive relationship between cytotoxic activity and molecular orbital energy such as pi-LUMO, pi-HOMO, and lone pair orbitals originated from O, N1, and N3 atoms. The present study demonstrated that cytotoxic activity of "half-mustard type" phenothiazines can be predicted by their dipole moments and molecular orbital energies.
A pharmacokinetic model to document the actual disposition of topical ivermectin incattle (2003)

Ivermectin is a worldwide-used antiparasitic drug largely administered to cattle as a topical formulation (pour-on). The actual plasma and faecal disposition of pour-on ivermectin in cattle was documented using an original pharmacokinetic model, and taking into account the oral ingestion of the topical drug following physiological licking as a secondary route of exposure. Six pairs of monozygotic twin cattle received successively one i.v. and two pour-on administrations of ivermectin at a 3-5-month interval. For one pour-on administration, the twins were separated into an unrestrained group and a group where self- and allo-licking were prevented. Ivermectin concentrations in the plasma and faeces were determined by HPLC. Licking resulted in a high intra-and inter-individual variability of systemic exposure after topical application. By the means of pharmacokinetic modelling, we showed that 58-87% of the pour-on dose was ingested, while only 10% was absorbed percutaneously. Approximately 72% of the ingested ivermectin transited directly into the faeces, resulting in a 7-fold higher faecal excretion of the parent drug than in the non-lickers. We conclude that topical administration does not guarantee a controlled drug delivery in cattle. More importantly, the simulations revealed that non-treated cattle could get easily contaminated by allo-licking, raising the public health problem of unexpected drug residues in edible tissues.
Synthesis, characterization, and antibacterial activity of vanadium(IV) complexes of hydroxamic acids (2009)

Abstract Vanadium(IV) complexes of composition [VCl2− n (acac)2(HL1,2) n ] (1–4) (where acac = ()HL1 = C6H5OCH2C(O)NHO−; phenoxyacetohydroxamate ion; HL2 = C6H5CH = CHC(O)NHO−; cinnamylhydroxamate ion, n = 1 and 2 have been synthesized by reaction of [VCl2(acac)2] with the potassium salt of phenoxyacetohydroxamic and cinnamylhydroxamic acids in predetermined molar ratios in THF + MeOH. The characterization of the complexes has been accomplished by elemental analyses, molar conductivity, magnetic measurements, IR, electronic, and mass spectral studies. Based upon spectral and molecular modeling dynamics, a distorted octahedral geometry around vanadium has been proposed. The thermal behavior of the complexes has been studied by TG and DTA techniques. The antibacterial activities of the newly synthesized complexes, vanadium precursor, and ligands have been assayed by the minimum inhibitory concentration method. The complexes have improved antibacterial activity over the free ligands. Keywords: Potassium salts of phenoxyacetohydroxamic and cinnamylhydroxamic acidsNon-oxovanadium(IV) complexesAntibacterial activity Acknowledgments Meena Kumari is grateful to University Grant Commission, New Delhi, for providing the financial support in the form of Major Research Project F. No.33-295/2007 (SR) dated 28.02.2008. The authors thank Department of Science & Technology (DST), Government of India, New Delhi, for providing the financial assistance for FT-IR and UV-Vis spectrophotometer facility to the department, Sophisticated Analytical Instrument Facility of CDRI, Lucknow, and Punjab University, Chandigarh for recording mass spectra and elemental analysis. The authors also thank IIT, Roorkee, for magnetic susceptibility measurements and Department of Biotechnology, Himachal Pradesh University, Shimla, for providing laboratory facilities.
Regionalization of the forebrain from neural plate to neural tube. (1995)

The vertebrate central nervous system is subdivided into distinct regions along the anteroposterior axis. It has been suggested that segmentation may play an important role in the regionalization and patterning of the neural tube, in particular in the development of the hindbrain. Recent evidence suggests that this may be true also in the forebrain. Several criteria that have been used to define segments and that have led to different views on how the anterior neural tube is subdivided will be discussed. However, regionalization of the neural ectoderm is a gradual and continuous process that starts as early as the induction of neural ectoderm. At the neural plate stage, distinct domains of gene expression have been established, indicating that a certain amount of regionalization has already taken place. Segmentation, as defined by lineage restriction between proposed segments or by the formation of morphological segment boundaries, is not thought to have occurred at the neural plate. When viewed in this context, segmentation may be considered a relatively late event in the ontogenesis of the nervous system. The early pattern of gene expression, the organization of the neural plate, and the underlying signals and tissue interactions may provide us with a better understanding of how the forebrain is regionalized.
Design of an Improved Type Rotary Inductive Coupling Structure for Rotatable Contactless Power Transfer System (2015)

This paper is aimed at analyzing the rotary inductive coupling structure of contactless rotary transformer. The main feature of the proposed rotatable contactless power transfer system is which winding is coaxial-interlayered for improving the magnetic coupling capability. There is no ferrite core used in the secondary-side of the rotary inductive coupling structure, this helps to ease the exerted force that is stress by the secondary-side on spindle. In order to verify the feasibility of the proposed contactless power transfer system for rotary applications, an inductive powered rotary machinery and the control system have been integrated. The experimental results show that the maximum power transfer efficiency of the proposed rotary inductive coupling structure is about 94.8%. The maximum output power received in the load end is 1030 W with transmission efficiency of 88%.
Post-Ischemic Helium Provides Neuroprotection in Rats Subjected to Middle Cerebral Artery Occlusion-Induced Ischemia by Producing Hypothermia (2009)

During the past decade, studies on the manipulation of various inhaled inert gases during ischemia and/or reperfusion have led to the conclusion that inert gases may be promising agents for treating acute ischemic stroke and perinatal hypoxia-ischemia insults. Although there is a general consensus that among these gases xenon is a golden standard, the possible widespread clinical use of xenon experiences major obstacles, namely its availability and cost of production. Interestingly, recent findings have shown that helium, which is a cost-efficient inert gas with no anesthetic properties, can provide neuroprotection against acute ischemic stroke in vivo when administered during ischemia and early reperfusion. We have investigated whether helium provides neuroprotection in rats subjected to middle cerebral artery occlusion (MCAO) when administered after reperfusion, a condition prerequisite for the therapeutic viability and possible clinical use of helium. In this study, we show that helium at 75 vol% produces neuroprotection and improvement of neurologic outcome in rats subjected to transient MCAO by producing hypothermia on account of its high specific heat as compared with air.