gebhart/patrepeval huggingface dataset:
An shRNA silencing a non‐toxic transgene reduces nutrient consumption and increases production of adenoviral vectors in a novel packaging cell (2008)

During production of an adenoviral vector in a packaging cell, transgene is expressed concomitantly with the adenoviral gene. Depending on the protein encoded by the transgene, the yield of an adenoviral vector can be reduced or blocked as a result of transgene expression in the packaging cell. We tested the effect of a short hairpin RNA (shRNA) inhibiting the expression of a transgene encoding hIcon, a therapeutic molecule that selectively destroys pathological angiogenic blood vessels, on the yield of an adenoviral vector containing the transgene. The results showed that the yield of infectious adenoviral vector particles was increased about 10-fold in a novel packaging cell with stable production of an shRNA that can silence the transgene, as compared to the yield in standard packaging line, and the consumption of nutrient in the novel packaging cell line is decreased due to silence of adenoviral transgene expression. The study indicates that shRNA can increase the production of adenoviral vectors by silencing transgene and reducing nutrient consumption of the novel packaging cell. The use of shRNA silencing expression of transgenes encoding therapeutic molecules can reduce the time and cost of producing adenoviral vectors for clinical use.
MMP-inhibitory effect of chlorhexidine applied in a self-etching adhesive. (2011)

When incorporated in a two-step self-etching adhesive primer (Clearfil SE Bond primer), chlorhexidine can partially maintain its inhibitory effect on MMPs.
Test for Temporal or Spatial Restrictions in Gene Product Function During the Cell Division Cycle (1983)

The ability of a functional gene to complement a nonfunctional gene may depend upon the intracellular relationship of the two genes. If so, the function of the gene product in question must be limited in time or in space. CDC (cell division cycle) gene products of Saccharomyces cerevisiae control discrete steps in cell division; therefore, they constitute reasonable candidates for genes that function with temporal or spatial restrictions. In an attempt to reveal such restrictions, we compared the ability of a CDC gene to complement a temperature-sensitive cdc gene in diploids where the genes are located within the same nucleus to complementation in heterokaryons where the genes are located in different nuclei. In CDC X cdc matings, complementation was monitored in rare heterokaryons by assaying the production of cdc haploid progeny (cytoductants) at the restrictive temperature. The production of cdc cytoductants indicates that the cdc nucleus was able to complete cell division at the restrictive temperature and implies that the CDC gene product was provided by the other nucleus or by cytoplasm in the heterokaryon. Cytoductants from cdc28 or cdc37 crosses were not efficiently produced, suggesting that these two genes are restricted spatially or temporally in their function. We found that of the cdc mutants tested 33 were complemented; cdc cytoductants were recovered at least as frequently as CDC cytoductants. A particularly interesting example was provided by the CDC4 gene. Mutations in CDC4 were found previously to produce a defect in both cell division and karyogamy. Surprisingly, the cell division defect of cdc4 nuclei is complemented by CDC4 nuclei in a heterokaryon, whereas the karyogamy defect is not.
Novel 5-HT<sub>7</sub>Receptor Inverse Agonists. Synthesis and Molecular Modeling of Arylpiperazine- and 1,2,3,4-Tetrahydroisoquinoline-Based Arylsulfonamides (2004)

A series of arylpiperazine- and 1,2,3,4-tetrahydroisoquinoline-based arylsulfonamides was synthesized and evaluated for their interactions with the constitutively active 5-HT7 receptor. Effects on basal adenylate cyclase activity were measured using HEK-293 cells expressing the rat 5-HT7. All ligands produced a decrease of adenylate cyclase activity, indicative of their inverse agonism. Additionally, computational studies with a set of 22 inverse agonists, including these novel inverse agonists and inverse agonists known from literature, resulted in a pharmacophore model and a CoMFA model (R2 = 0.97, SE = 0.18). Docking of inverse agonists at the binding site of a model of the helical parts of the 5-HT7 receptor, based on the alpha carbon template for 7-TM GPCRs, revealed interesting molecular interactions and a possible explanation for observed structure-activity relationships.
Mobile ad hoc networking: milestones, challenges, and new research directions (2014)

In this article we discuss the state of the art of (mobile) multihop ad hoc networking. This paradigm has often been identified with the solutions developed inside the IETF MANET working group, and for this reason it is called the MANET paradigm. However, they do not coincide, and in the last decade they clearly diverged. In this article, we start from the reasons why the MANET paradigm did not have a major impact on computer communications, and we discuss the evolution of the multihop ad hoc networking paradigm by building on the lessons learned from the MANET research. Specifically, we analyze four successful networking paradigms, mesh, sensor, opportunistic, and vehicular networks, that emerged from the MANET world as a more pragmatic application of the multihop ad hoc networking paradigm. We also present the new research directions in the multihop ad hoc networking field: peoplecentric networking, triggered by the increasing penetration of the smartphones in everyday life, which is generating a people-centric revolution in computing and communications.
Range of Motion Studies for Total Hip Replacements (1975)

Significant differences in ROM exist between different THR prosthesis designs: several of the prosthesis designs tested are marginal in flexion; several millimeters of socket wear will decrease the ROM. The results also emphasize the importance of proper component orientation at surgery. The surgeon has less latitude in orienting the components of a THR with limited ROM. Subluxation and dislocation due to rim contact can be minimized with most prosthetic units by instructing the patients to abduct and/or externally rotate their hips during acute flexion. Analyses suggest that impingement of prosthesis neck and socket rim may lead to increased risk of dislocation and increased rim wear. Prostheses with adequate ROM for everyday activities should provide stability, less frequent neck and socket contact with decreased rim wear, less force transmission to acrylic-bone interface, and less diminution of ROM with wear of the socket wall.
STT-MRAM for embedded memory applications from eNVM to last level cache (2017)

Spin-Transfer-Torque Magnetic Random Access Memory (STT-MRAM) is emerging as a leading candidate for a variety of embedded memory applications ranging from embedded NVM to working memory and last level cache. In this paper, we review recent breakthroughs that have brought perpendicular STT-MRAM to the cusp of mass production.
Dynamics of vibrationally excited ozone formed by three-body recombination. II. Kinetics and mechanism (1987)

Spectrally resolved infrared chemiluminescence from vibrationally excited ozone, O3(v), has been used to study the reaction kinetics of O3(v) in discharged O2/Ar mixtures at ∼1 Torr and 80–150 K. Dependences of the excited state number densities on temperature and O2 mole fraction indicate O3(v) is formed primarily by three-body recombination of O with O2 and is destroyed by rapid chemical reaction with O. Several secondary excitation reactions involving vibrationally and electronically excited O2 are also indicated. The data are treated with a detailed steady-state analysis of the discharge kinetics, to extract estimates for rate coefficients of the key elementary reactions. The effective ‘‘quasinascent’’ state distribution in recombination is also inferred; this distribution shows selective recombination into the asymmetric stretching mode, but an apparently statistical (i.e., collisionally scrambled) behavior among the vibrational states within that mode. The results are discussed in terms of the detailed dynamics of three-body recombination.
On the Relationship between Economic Law and Civil Law (2005)

Economic law and civil law are important law department which adjust market dealing and economic run. They are important indemnify of market economic development. There are both correspondent and difference between two relationship. Recognizing these, it is important meaning on propelling economic development.
Biomechanical Stability of Four Cranial Cruciate Ligament Repair Techniques in the Dog (1991)

Four commonly used cranial cruciate ligament (CCL) repair techniques were compared bio‐mechanically in canine cadaver stifles by assessment of immediate postoperative laxity (cranial drawer) and stiffness. Each joint was tested nondestructively before repair, with the CCL intact and with the CCL excised. The cranio‐caudal laxity after each repair was expressed as a percentage of the laxity in the same stifle after CCL excision. The stiffness of a repair was expressed as a percentage of the same intact joint's stiffness at an equivalent displacement. Repaired stifles were tested three consecutive times to the same maximum load (180 N) and the comparative laxity and stiffness in each test were determined. Fibular head transposition resulted in significantly increased stiffness, less laxity, and less decay per test than the other repairs. Lateral imbrication resulted in significantly less laxity on all tests, and significantly greater stiffness than the fascial strip or patellar tendon on the second and third tests to maximum load. The fascial strip resulted in significantly less laxity on all tests than the patellar tendon, but it was not significantly greater in stiffness. These data suggest that, of the reconstructive methods tested, fibular head transposition has biomechanically superior properties in the immediate postoperative period.
Surface-Enhanced Raman Scattering of Biological Molecules on Metal Colloid II: Effects of Aggregation of Gold Colloid and Comparison of Effects of pH of Glycine Solutions between Gold and Silver Colloids (1999)

As the second paper of a series of our studies on surface-enhanced Raman scattering (SERS) of biological molecules on metal colloid, this paper reports effects of aggregation of gold colloid on the SERS intensity of glycine (Gly) in aqueous solutions and comparison of effects of pH of the Gly solutions between gold and silver colloids. In order to investigate the relation between the SERS effect and colloidal aggregation, we have developed a spectroscopic system by which we can measure simultaneously time-resolved SERS spectra and a time-dependent intensity change in a surface plasmon absorption of a metal colloid system. By use of this instrument we monitored time-dependent intensity changes in the SERS signals and in an absorption band at 802 nm of Gly on gold colloid at pH 3.9 and 6.0. For both pH values, the intensities of the SERS and the absorption band change in concert with each other at the emergence of the SERS effect. The intensity changes in the SERS signals are rather slow for the solution of pH 6.0 while rapid for that of pH 3.9. The coagulation proceeds slowly near the isoelectric point of Gly, whereas the rapid coagulation happens in the acidic region. SERS spectra of Gly adsorbed on silver colloid were measured at various pH values, and it was found that, in sharp contrast to the gold colloid system, which we reported in our previous paper, the SERS intensity of Gly on the silver colloid decreases markedly at pH 2.0 where most of Gly in the solution has a COOH group instead of a COO − group. It seems that the coagulation of the silver colloid particles does not occur very much below p K 1 (2.35) because the COOH groups do not interact strongly with the positively charged silver particles.
A General Synthesis of 4-Substituted 6-(2-Imidazolinylamino)-5,8-dimethylquinolines (1998)

A general synthesis of 4-substituted 6-(2-imidazolinylamino)-5,8-dimethylquinolines 1 has been developed. All new compounds were synthesized from a common intermediate, 5,8-dimethyl-6-nitro-4-quinolone 3, the structure of which was confirmed by X-ray crystallography. This methodology involved the conversion of 3 into either a 4-chloro- or 4-bromoquinoline followed by the introduction of various 4-substituents late in the synthetic sequence. Substituents introduced in this way include alkyl (18a), alkoxy (12a, 12b), halo (9, 12c, 16), cyano (18b), thioalkyl (12d), acetamido (14), carboxamido (19), and hydroxy (10). This work illustrates the utility of 4-haloquinoline intermediates in the general synthesis of 4-substituted quinolines.
Remote sensing of NO using a differential absorption lidar (1980)

Single-ended remote sensing measurements of atmospheric NO have been made using differential absorption of frequency-doubled pulsed CO(2) laser radiation backscattered from topographic targets. Returns were obtained from targets at ranges out to 1.4 km, and significant NO concentrations above ambient were observed over a path which crossed a traffic roadway at a range of 0.5 km. In view of the severe atmospheric water vapor absorption in the spectral region containing the NO absorption band, the range dependence of the lidar returns was also measured in order to determine the differential absorption of the ambient atmosphere.The results differed significantly from those computed from atmospheric transmission data tapes.
An experimental evaluation of a group- versus computer-based intervention to improve food portion size estimation skills (2005)

The ability to accurately estimate and measure food portion sizes is important for preventing and treating obesity. This study describes the development, implementation and evaluation of a group- versus computer-based intervention to improve food portion estimation abilities using real food and food models. A convenience sample of 76 women was randomly assigned to one of three conditions: computer training, group training or a waitlist control condition. Assessments at baseline and 2 weeks post-intervention included portion size testing using real foods and food models, self-efficacy for judging portion sizes and using measuring utensils, and knowledge of portion information. At baseline, greater estimation errors were observed for amorphous foods. No group by time interaction was observed on estimation of real foods; however, both the computer and group training resulted in significant improvements in estimating the size of food models, greater self-efficacy for judging portion sizes and more accurate knowledge of portion information compared with the control condition. Process measures indicated that the group training was deemed more helpful and more personally relevant to the participants.
Nonreciprocal Packaging of Human Immunodeficiency Virus Type 1 and Type 2 RNA: a Possible Role for the p2 Domain of Gag in RNA Encapsidation (1998)

The ability of human immunodeficiency virus types 1 (HIV-1) and 2 (HIV-2) to cross-package each other's RNA was investigated by cotransfecting helper virus constructs with vectors derived from both viruses from which the gag and pol sequences had been removed. HIV-1 was able to package both HIV-1 and HIV-2 vector RNA. The unspliced HIV-1 vector RNA was packaged preferentially over spliced RNA; however, unspliced and spliced HIV-2 vector RNA were packaged in proportion to their cytoplasmic concentrations. The HIV-2 helper virus was unable to package the HIV-1 vector RNA, indicating a nonreciprocal RNA packaging relationship between these two lentiviruses. Chimeric proviruses based on HIV-2 were constructed to identify the regions of the HIV-1 Gag protein conferring RNA-packaging specificity for the HIV-1 packaging signal. Two chimeric viruses were constructed in which domains within the HIV-2 gag gene were replaced by the corresponding domains in HIV-1, and the ability of the chimeric proviruses to encapsidate an HIV-1-based vector was studied. Wild-type HIV-2 was unable to package the HIV-1-based vector; however, replacement of the HIV-2 nucleocapsid by that of HIV-1 generated a virus with normal protein processing which could package the HIV-1-based vector. The chimeric viruses retained the ability to package HIV-2 genomic RNA, providing further evidence for a lack of reciprocity in RNA-packaging ability between the HIV-1 and HIV-2 nucleocapsid proteins. Inclusion of the p2 domain of HIV-1 Gag in the chimera significantly enhanced packaging.
Spectral Methods for Determination of Water (1988)

Techniques based on the interaction of electromagnetic radiation with materials can be extremely useful for the characterization of water in solids of pharmaceutical interest. When combined with other physical methods, such as calorimetry, thermogravimetry, or titration, spectral techniques can be used to deduce information regarding the nature of water contained within a solid. The spectral methods are particularly useful in the study of hydrate species, and in the differentiation of hydrate water from entrapped or adventitious water. The most useful methods are powder x-ray diffraction, fourier transform infrared spectroscopy, and solid state nuclear magnetic resonance spectroscopy. These particular methods have been used in the study of ampicillin hydrate species.
Efficacy and safety of a calcium sensitizer, levosimendan, in patients with right heart failure due to pulmonary hypertension (2017)

Intravenous levosimendan can effectively improve severe RHF of PH patients in hospital and well tolerated.
Evaluation of Strategies for Crossbreeding of Dairy Cattle in Brazil (1990)

To compare breeding strategies, ec0nomic perfonnance was calculated for 376 cows of six red and white Holstein-Friesian x Guzera crossbred groups (1/4 to ~1/32 European grades), based on their accwnulated dairy production (914 lactations on 60 fanns) and on culling or death observations of 87 nonfreshening heifers. Perfonnance was predicted from a genetic model based on additive-dominance and inter se mating effects for the following: utilization of Fl females, upgrading to Holstein-Friesian, new synthetic breed, crisscrossing, and modified crisscrossing (of Holstein-Friesian sires for two generations and zebu sires for one generation). On the better-managed fanns, profit per day of herd life for those strategies, was equivalent to, respectively, 1.82, 1.36, -.33, .75, and 1.36 kg of milk, whereas corresponding equivalence on low management level fanns was 4.64, -.95, 1.37,2.72, and 2.23 kg. Differences between groups in culling and mortality rates were considerable in the low management level, influencing herd life and heifer cost and reducing profit of high European grades. hnportant economic gains may accme from choice of ~bree<!ing strategy to match the appropnate ammal genetic resources to husbandry practices. Continuous PI heifer replacement programs may have sound economic basis, particularly for low management level fanns. Crisscrossing was the second
Title of Abstract: The efficiency of poly(hexamethylene biguanide) hydrochloride (PHMD) as a root canal irrigant (2011)

Aim was tTo test the efficacy of poly(hexamethylene biguanide) hydrochloride (PHMD) as a root canal irrigant against E.faecalis and P.aeruginosa in an experimental model ex vivo. Crowns and apical parts of twenty-two extracted single rooted human teeth were removed with diamond burs and root canals were instrumented with Hedstrom files to size #40. Smear layer was removed with sequential usage of 17% EDTA and 2.50% NaOCl and then sterilized in autoclave on 121oC for 15 minutes. Specimens were randomly divided into even groups and mounted with sticky-wax at the bottom of cell culture wells. Eleven specimens were inoculated with an overnight broth culture of E.faecalis (ATCC 51299) and eleven with P.aeruginosa (ATCC27853). After 48 h incubation and irrigation with saline dentin samples were collected from inner root canal walls with Hedstrom files #50 before and after irrigation with 10 mL of 1% PHMD (2 mL every 2 minutes) followed by irrigation with 2 mL saline. Samples were put into vials containing 2 mL of sterile saline and tenfold dilutions, were plated on blood agar (Oxoid, UK) after 30s vortexing. Colony forming units (CFU) were counted after 48h incubation at 37oC. Wilcoxon Signed Ranks test was used for statistical analysis. After treatment with PHMD P.aeruginosa was eradicated from all specimens and E.faecalis from 8 of 10 specimens. In two samples that grew E.faecalis after treatment reduction was from 104 CFU/mL to 101 CFU/mL. These results were statistically significant for both groups of microorganisms tested (P<0.01). PHMD in concentration 1% effectively reduces the number of E.faecalis and P.aeruginosa in an experimental tooth model and therefore its role as an endodontic irrigant should be studied further.
A Time-Gated Hybrid CMOS Sensor with Multi-Frame Storage for High-Speed X-Ray Imaging. (2014)

Abstract not provided.
Learning to Generate Long-term Future via Hierarchical Prediction (2017)

We propose a hierarchical approach for making long-term predictions of future frames. To avoid inherent compounding errors in recursive pixel-level prediction, we propose to first estimate high-level structure in the input frames, then predict how that structure evolves in the future, and finally by observing a single frame from the past and the predicted high-level structure, we construct the future frames without having to observe any of the pixel-level predictions. Long-term video prediction is difficult to perform by recurrently observing the predicted frames because the small errors in pixel space exponentially amplify as predictions are made deeper into the future. Our approach prevents pixel-level error propagation from happening by removing the need to observe the predicted frames. Our model is built with a combination of LSTM and analogy based encoder-decoder convolutional neural networks, which independently predict the video structure and generate the future frames, respectively. In experiments, our model is evaluated on the Human3.6M and Penn Action datasets on the task of long-term pixel-level video prediction of humans performing actions and demonstrate significantly better results than the state-of-the-art.
Directional Difference of Conduction Velocity in the Cardiac Ventricular Syncytium Studied by Microelectrodes (1959)

In the theory of electrocardiography the direction of myocardial fibers has been almost ignored. In order to evaluate whether this is proper or not, directional difference of conduction velocity in the ventricular syncytium was examined by capillary microelectrodes. Conduction velocity in the direction of myocardial fibers was found to be usually several times larger than that vertical to them. This difference is significantly large and cannot be ignored even though rapidity of conduction through Purkinje fibers is considered. This statement became more applicable in various abnormal conditions. Thus it is concluded that the direction of myocardial fibers should be given more attention in discussion of propagation of excitation waves in the cardiac ventricle.