gebhart/patrepeval huggingface dataset:
Progressive growth in immunodeficient mice and host cell recruitment by mouse endothelial cells transformed by polyoma middle-sized T antigen: implications for the pathogenesis of opportunistic vascular tumors. (1994)

A retroviral construct encoding polyoma middle-sized T antigen was used to generate transformed endothelial cell lines from heart (H5V), brain (B9V), and whole-embryo (E10V) of C57BL/6 mice. When injected into syngeneic recipients, H5V and the less studied B9V and E10V cells caused vascular tumors which, depending on the number of cells inoculated, regressed or progressed, leading to death of the host. When H5V cells were injected into immunodeficient mice, tumors were observed with inocula which did not form lesions in immunocompetent recipients and regression did not occur. Treatment with anti-LFA-1, anti-Thy-1.2, and anti-CD8 antibodies abolished rejection; anti-CD4 was a somewhat less effective inhibitor of resistance. Animals with progressive tumors exhibited secondary lesions in various organs with prominent skin involvement in nude mice. Histologically, the tumors had the appearance of a hemangioma, with areas resembling Kaposi sarcoma. Cells lining vascular lacunae had the morphological features of injected H5V cells. The lesions were characterized by prominent neovascularization and mononuclear cell infiltration. Southern blot hybridization analysis revealed that approximately 5% of the cells in the tumor mass were transplanted H5V cells. Thus, the H5V transformed endothelial line causes vascular lesions that are sustained to a large extent by recruitment of host cells and manifests full malignant behavior only in immunocompromised hosts. The hypothesis of a tumor sustained by a minute proportion of transformed cells, which recruit host elements and express full malignant behavior only in immunodeficient hosts, would account for several features of some vascular neoplasms in man.
Altered Neural Cell Fates and Medulloblastoma in Mouse <i>patched</i> Mutants (1997)

The PATCHED (PTC) gene encodes a Sonic hedgehog (Shh) receptor and a tumor suppressor protein that is defective in basal cell nevus syndrome (BCNS). Functions of PTC were investigated by inactivating the mouse gene. Mice homozygous for the ptc mutation died during embryogenesis and were found to have open and overgrown neural tubes. Two Shh target genes, ptc itself and Gli, were derepressed in the ectoderm and mesoderm but not in the endoderm. Shh targets that are, under normal conditions, transcribed ventrally were aberrantly expressed in dorsal and lateral neural tube cells. Thus Ptc appears to be essential for repression of genes that are locally activated by Shh. Mice heterozygous for the ptc mutation were larger than normal, and a subset of them developed hindlimb defects or cerebellar medulloblastomas, abnormalities also seen in BCNS patients.
Effect of Herpes Simplex Suppression on Incidence of HIV among Women in Tanzania (2008)

These data show no evidence that acyclovir (400 mg twice daily) as HSV suppressive therapy decreases the incidence of infection with HIV. (Current Controlled Trials number, ISRCTN35385041 [controlled-trials.com].).
Tolerance of Simulated Amniotic Fluid in Premature Neonates (2002)

Enteral administration of an amniotic fluid-like solution to preterm neonates is well tolerated in doses <or=20 mL/kg/d.
A Monocyte Chemoattractant Protein-1 (MCP-1) Polymorphism And Outcome After Renal Transplantation (2002)

Among the factors modulating transplant rejection and cardiovascular disease, chemokines and their respective receptors deserve special attention. In this respect, increased expression of MCP-1 and the corresponding receptor CCR2 have been demonstrated in renal transplant rejection and coronary artery disease. The impact of the MCP-1-2518G and CCR2-64I genotypes on renal allograft function was investigated in 232 patients who underwent transplantation over an 11-yr period. Genomic DNA was genotyped using PCR with sequence-specific primers followed by restriction fragment length polymorphism analysis. Eighteen (7.8%) patients were homozygous for the MCP-1-2518G mutation. The G/G allele of MCP-1 -2518 behaved as a determinant for long-term allograft survival and resulted in reduction of the mean graft survival, as compared with the heterozygous (A/G) or wild-type (A/A) allele (67 +/- 14 versus 95 +/- 4 mo; Log rank P = 0.0052). The 64I mutation of CCR2 had no effect on kidney graft failure (93 +/- 6 and 91 +/- 5 mo, respectively; P = 0.81). None of the investigated polymorphisms showed a significant shift in gene frequency in acute rejection and rejection-free groups. In conjunction with these findings, peripheral blood mononuclear cells from kidney transplant recipients carrying the G-allele were characterized by a 2.5-fold higher MCP-1 secretion (P < 0.05). In conclusion, recipients of renal transplants homozygous for the -2518 G mutation of the MCP-1 gene are at risk for premature kidney graft failure. This variant of MCP-1 may be a future predictor for long-term kidney graft failure.
Feeding strategies for 2012 (2012)

This information was part of the February 2012 issue of Eastern DairyBusiness Magazine. The Manager, a section within the Eastern DairyBusiness Magazine, is authored and organized by the PRO-DAIRY program in the College of Agriculture and Life Sciences at Cornell University.
Performance-Based Gas Turbine Health Monitoring, Diagnostics, and Prognostics: A Survey (2018)

Health monitoring is an essential part of condition-based maintenance and prognostics and health management for gas turbines. Various health monitoring systems have been developed based on the measurement and observation of the fault symptoms including turbine performance parameters such as heat rate, and nonperformance symptoms such as structural vibration. This paper focuses on surveying state-of-the-art condition monitoring, diagnostic and prognostic techniques using performance parameters acquired from gas-path data that are mostly available from the operating systems of gas turbines. Performance parameters and the corresponding effective factors are presented in the beginning. Structure of performance monitoring and diagnostic systems are systematically laid out next, and the recent developments in each section are surveyed and discussed. Observing the importance of the prognostics in the recent trend of health monitoring research, an emphasis is given on the prognostic frameworks and their implementation for the remaining useful life prediction. A conclusion along with a brief discussion on the current state and potential future directions is provided at the end.
The Gangs of New Labour: yobs or just youth? (2003)

(2003). The Gangs of New Labour: yobs or just youth? Criminal Justice Matters: Vol. 54, Youth and Crime, pp. 10-11.
Sensitivity Enhancement of Wheatstone Bridge Circuit for Resistance Measurement (2009)

Present work deals with the development of a low cost, appreciably accurate and sensitive electronic circuit for resistive sensor. The circuit is based on the modification of the Wheatstone bridge using active devices. It helps measurement of the incremental resistance precisely and linearly. It requires few components for its hardware implementation and found to be suitable in case there is small change in resistance due to change in physical quantity or chemical analytes to be measured. Theory of the proposed bridge circuit has been discussed and experimental results have been compared with conventional full bridge circuit. Experiments have been conducted with metallic strain gauge sensor but it can be utilized to other resistive sensors. Results show that the output of the circuit is almost four times more than usual full Wheatstone bridge circuit. Experimental results show that the errors due to the effects of the ambient temperature and connecting lead resistance are minimized. Copyright © 2009 IFSA.
In loving memory of Professor Pam Gilbert (nee Phillips) 12/5/1946-13/11/2002 (2003)

Staff in the School of Education at James Cook University were deeply saddened by Pare Gilbert's death on 13 November 2002 at her home in Townsville. I still find it difficult to believe that she is not with us. Pam worked until the end, writing, supervising research students, teaching and talking with colleagues, running research projects. There are still so many reminders in our daily working lives of her vitality and wit, of her intellect and humour, of her thoughtfulness and good sense. I first met Pam at a conference in Perth in 1990. Pam was a keynote speaker and the star of the show. I was a novice academic, newly appointed to a university position following several years teaching in high schools. Pare had sought me out by asking a conference organiser to introduce us. I was stunned. Pam was a giant in the academic world I was only just entering. Internationally recognised, lauded in the UK, Canada, New Zealand the USA, Pam's work was cited in the leading journals in her field, she had published several scholarly books, every serious scholar in English and literacy education had read her work, and she was the only reason I knew about James Cook University. I was so nervous that I barely remember anything about our conversation, but I was reminded of it recently while listening to Pam's husband, Rob Gilbert, speak at her funeral. Rob commented then that he believed Pam had no interest at all in promoting herself. Despite all the honours and plaudits and invitations to speak at so many notable occasions ... she really had no interest in being the centre of attention. Rather, she was always more interested in what others were doing, and if she could help them. That certainly was my experience on that memorable day when I had coffee with the famous researcher from a place called Townsville, whose work I had had to get my head around in order to write my own thesis. Pam's collegiality and generosity in sharing ideas and exploring the boundaries of her field had no limits and her humble approach to her own achievements set a rare benchmark in university circles. A significant feature of Pam's career is that her work is widely acknowledged as innovative and relevant by professional educators, academics and students alike. Her extraordinary career spanned twenty years teaching at high schools and another twenty years as an academic at JCU. During her school teaching career Pare held teaching positions at Ayr, Townsville, Brisbane, Ipswich, and Nambour. She was Head of English at the then Heatley High School, and taught in London during the late 1970s while also studying at the London Institute of Education. …
Bilateral Sensorineural Hearing Loss in Childhood – Three Cases Presenting in British Forces Germany (1990)

Three cases of bilateral sensorineural deafness detected at various stages of childhood in British Forces Germany (BFG) illustrate the importance of providing appropriate audiological screening for all children in the Services and muItidisciplinary assessment for any child with suspected developmental delay.
GRIM-1, a Novel Growth Suppressor, Inhibits rRNA Maturation by Suppressing Small Nucleolar RNAs (2011)

We have recently isolated novel IFN-inducible gene, Gene associated with Retinoid-Interferon-induced Mortality-1 (GRIM-1), using a genetic technique. Moderate ectopic expression of GRIM-1 caused growth inhibition and sensitized cells to retinoic acid (RA)/IFN-induced cell death while high expression caused apoptosis. GRIM-1 depletion, using RNAi, conferred a growth advantage. Three protein isoforms (1α, 1β and 1γ) with identical C-termini are produced from GRIM-1 mRNA. We show that GRIM-1 isoforms interact with NAF1 and DKC1, two essential proteins required for box H/ACA sno/sca RNP biogenesis and suppresses box H/ACA RNA levels in mammalian cells by delocalizing NAF1. Suppression of these small RNAs manifests as inefficient rRNA maturation and growth suppression. Interestingly, yeast Shq1p also caused growth suppression in mammalian cells. Consistent with its growth-suppressive property, GRIM-1 expression is lost in a number of human primary prostate tumors. Our observations support a recent study that GRIM-1 might act as a co-tumor suppressor in the prostate.
Development of a Human Gamma Interferon Enzyme Immunoassay and Comparison with Tuberculin Skin Testing for Detection of<i>Mycobacterium tuberculosis</i>Infection (1998)

A sensitive two-step simultaneous enzyme immunoassay (EIA) for human gamma interferon (IFN-gamma) has been developed and used as an in vitro test for human tuberculosis (TB) in comparison with tuberculin skin testing. The EIA was shown to be highly sensitive, detecting less than 0.5 IU of recombinant human IFN-gamma per ml within a linear detection range of 0.5 to 150 IU/ml. The assay was highly reproducible and specific for native IFN-gamma. In addition, the assay detected chimpanzee, orangutan, gibbon, and squirrel monkey IFN-gammas. Cross-reactions with other human cytokines or with IFN-gammas derived from mice, cattle, or Old World monkeys were not evident. The assay was used to detect TB infection by incubating whole blood overnight with human, avian, and bovine tuberculin purified protein derivatives (PPDs), as well as positive (mitogen)- and negative-control preparations. The levels of IFN-gamma in plasma supernatants were then determined. Blood from 10 tuberculin skin test-positive individuals responded predominantly to the human tuberculin PPD antigen and to a lesser extent to bovine and avian PPD antigens. By contrast, blood from 10 skin test-negative individuals showed minimal responses or no response to any of the tuberculin PPDs. Detectable levels of IFN-gamma were present in all blood samples stimulated with mitogen. In vivo tuberculin reactivity was correlated with IFN-gamma responsiveness in vitro. These results support the further study of the blood culture-IFN-gamma EIA system as an alternative to skin testing for the detection of human TB infection.
Adsorption Kinetics of Alcohols on Single-Wall Carbon Nanotubes: An Ultrahigh Vacuum Surface Chemistry Study (2008)

Presented are thermal desorption spectroscopy (TDS) data of alcohol adsorption on single-wall carbon nanotubes (CNTs) supported on silica. The adsorption kinetics of methanol, ethanol, propanol, 2-propanol, butanol, pentanol, and hexanol have been studied. Multimass TDS confirms molecular adsorption/desorption with low coverage binding energies, E0, increasing linearly with the number of carbons, n. The coverage, Θ, dependence of the heat of adsorption, Ed(Θ), has been obtained by a Redhead analysis. The heat of sublimation increases linearly with n whereas the zeroth-order pre-exponential factor is approximately independent of n. In addition, TDS of alkanes (butane, pentane, hexane, trimethylpentane) as well as alcohol−alkane coadsorption data have been collected to characterize possible adsorption sites. Alkane TDS leads to fingerprint curves indicating three different adsorption sites (external, groove, internal) on CNTs, in agreement with earlier studies. For most of the alcohols only one monolayer TDS peak is seen indicating a dominance of lateral interactions. However, the coadsorption data provide evidence for alcohol adsorption on interior sites of the CNTs.
The latency pattern of Epstein–Barr virus infection and viral IL-10 expression in cutaneous natural killer/T-cell lymphomas (2001)

The nasal type, extranodal natural killer or T(NK/T)-cell lymphoma is usually associated with latent Epstein–Barr virus (EBV) infection. In order to elucidate the EBV gene expression patterns in vivo, we examined eight patients with cutaneous EBV-related NK/T-cell lymphomas, including six patients with a NK-cell phenotype and two patients with a T-cell phenotype. The implication of EBV in the skin lesions was determined by the presence of EBV-DNA, EBV-encoded nuclear RNA (EBER) and a clonality of EBV-DNA fragments containing the terminal repeats. Transcripts of EBV-encoded genes were screened by reverse transcription- polymerase chain reaction (RT-PCR), and confirmed by Southern blot hybridization. The expression of EBV-related antigens was examined by immunostaining using paraffin-embedded tissue sections and cell pellets of EBV-positive cell lines. Our study demonstrated that all samples from the patients contained EBV nuclear antigen (EBNA)-1 mRNA which was transcribed using the Q promoter, whereas both the Q promoter and another upstream promoter (Cp/Wp) were used in EBV-positive cell lines, B95.8, Raji and Jiyoye. Latent membrane protein-1 (LMP-1) mRNA was detected in seven of eight patients and all cell lines, whereas EBNA-2 transcripts were found only in the cell lines. Immunostaining showed no LMP-1, EBNA-2 or ZEBRA antigens in the paraffin-embedded tissue sections, although they were positive in the cell line cells. Latent BHRF1 transcripts encoding bcl-2 homologue and BCRF1 transcripts encoding viral interleukin (vIL)-10 were detected in one and two of eight patients, respectively. A patient with NK-cell lymphoma expressing both transcripts died of rapid progression of the illness. Our results indicate that the restricted expression of the latency-associated EBV genes and the production of vIL-10 and bcl-2 homologue may favour tumour growth, evading the host immune surveillance. © 2001 Cancer Research Campaign
Intradiscal drug delivery system for the treatment of low back pain (2009)

Possible solution to the long-term control of the low back pain (LBP) would be by using an injectable pain drug carrier that can be delivered locally. The drug can be released in a controlled manner. It is also allowed to inject repeatedly more drugs percutaneously with a minimal invasion. The main objective of this study was to develop such a drug delivery system (DDS) for long-term control of discogenic LBP. The DDS consists of in situ forming hydrogel matrix (Pluronic F127 plus sodium hyaluronate) containing microspheres (MS). The solid MS were used for long-term release of the drugs. Both hydrogel matrix and MS contained a model drug, bupivacaine base (BB). The phase transition (liquid at room temperature, gel at around body temperature) could be manipulated by changing the composition of the hydrogel. In vitro test showed that approximately 3% (w/w) of the BB loaded to MS were released during 42 days, demonstrating a good potential for sustained release of bupivacaine.
Fraternity Auditing Service (1942)

T HE period from 1920 to i928 was a building era for the Greek-letter organizations. By 1930 national fraternities owned 1,874 houses valued at $72,521,174.1 Many of these houses were financed when interest rates were high and when building materials were expensive. Incoming members of these organizations often shouldered enormous debts which had not been retired. Fraternities began to face new problems in attracting members. Some of the activities of these groups were absorbed by the colleges through the expansion of recreational and social activities and through the increased construction of dormitory facilities. The financial crises of i929, with the resulting depression which followed, added to the problems with which the fraternities were confronted and found many groups with insufficient income to meet operating expenses. Many organizations had to refinance, combine, or seek smaller quarters; some of them were forced to close. The instability of the organized houses often brought embarrassment to the college and student body. Mer-
Homogeneous Charge Compression Ignition of Diesel Fuel (1996)

<div class="htmlview paragraph">A single-cylinder, direct-injection diesel engine was modified to operate on compression ignition of homogenous mixtures of diesel fuel and air. Previous work has indicated that extremely low emissions and high efficiencies are possible if ignition of homogeneous fuel-air mixtures is accomplished. The limitations of this approach were reported to be misfire and knock. These same observations were verified in the current work. The variables examined in this study included air-fuel ratio, compression ratio, fresh intake air temperature, exhaust gas recirculation rate, and intake mixture temperatures. The results suggested that controlled homogeneous charge compression ignition (HCCI) is possible. Compression ratio, EGR rate, and air fuel ratio are the practical controlling factors in achieving satisfactory operation. It was found that satisfactory power settings are possible with high EGR rates and stoichiometric fuel-air mixtures. Successful HCCI operation results in near zero smoke emissions.</div>
Characterization of Ryanodine Receptor–mediated Calcium Release in Human B Cells (2006)

Background Mutations in the ryanodine type 1 receptor (RyR1) are causative for malignant hyperthermia. Studies in human B lymphocytes have shown that measurement of RyR1-mediated intracellular Ca(2+) (Ca(2+)(i)) release can differentiate between normal and malignant hyperthermia-susceptible individuals. The authors have further developed the B-cell assay by pharmacologically characterizing RyR1-mediated Ca release in two normal human B-cell lines and demonstrating increased sensitivity of lymphocytes to the RyR1 agonist 4-chloro-m-cresol (4-CmC) in the porcine model of MH. Methods Ca(2+)(i) was measured fluorometrically using fura-2 in populations of cells in suspension or with fluo-4 in single cells using confocal microscopy. The Dakiki and PP normal human B cell lines were used, as well as lymphocytes obtained from normal and malignant hyperthermia-susceptible pigs. 4-CmC was used to elicit RyR1-mediated Ca release; all experiments were performed in the absence of external Ca(2+). Results EC(50) values for 4-CmC were 0.98 and 1.04 mm for Dakiki and PP cells, respectively, demonstrating reproducibility. The 4-CmC-induced increase in Ca(2+)(i) was eliminated by thapsigargin and was unaffected by xestospongin C. The Ca(2+)(i) increase was separable from mitochondrial stores and was inhibited by azumolene. Caffeine did not induce Ca(2+)(i) release, but ryanodine depleted intracellular stores by 50%. Lymphocytes from pigs carrying the Arg614Cys mutation in RyR1 showed increased sensitivity to 4-CmC (EC(50) = 0.47 vs. 0.81 mm for cells derived from normal animals). Conclusions RyR1-mediated Ca(2+) signals can be pharmacologically distinguished from other intracellular sources in human B cells, and alterations of RyR1 function can be successfully detected using Ca(2+) release from intracellular stores as an end point.
Erythropoietin triggers a burst of GATA-1 in normal human erythroid cells differentiating in tissue culture (1993)

GATA-1 is a central transcription-activator of erythroid differentiation. In the present work we have studied the kinetics of its expression and activity during development of normal human erythroid progenitors, grown in primary cultures. In response to the addition of erythropoietin (Epo), the cells undergo proliferation and differentiation in a synchronized fashion. This recently developed experimental system allows biochemical dissection of erythroid differentiation in a physiological meaningful environment. No DNA-binding activity of GATA-1 could be detected before the addition of Epo, although a very low level of mRNA was observed. Following Epo addition there was a sharp parallel rise in both mRNA and DNA-binding activity, consistent with positive autoregulation of the GATA-1 gene. After reaching a peak on day 7-9, both mRNA and protein activity decreased. The binding activity of the ubiquitous factor SP1 showed a biphasic pattern; its second peak usually coincided with the GATA-1 peak, suggesting that SP1 also plays a specific role in erythroid maturation. The highest activity of GATA-1 per erythroid cell was found on day 6-8, immediately preceding the major rise in globin gene mRNA and in the number of hemoglobinized cells. The results imply that a high level of GATA-1 activity is necessary for globin gene expression and erythroid maturation, suggesting that a requirement for a threshold concentration of GATA-1 is part of the mechanism that determines the final steps of erythroid maturation.