gebhart/patrepeval huggingface dataset:
Guanosine Triphosphatase Activating Protein (GAP) Interacts with the p21 <i>ras</i> Effector Binding Domain (1988)

A cytoplasmic protein that greatly enhances the guanosine triphosphatase (GTPase) activity of N-ras protein but does not affect the activity of oncogenic ras mutants has been recently described. This protein (GAP) is shown here to be ubiquitous in higher eukaryotes and to interact with H-ras as well as with N-ras proteins. To identify the region of ras p21 with which GAP interacts, 21 H-ras mutant proteins were purified and tested for their ability to undergo stimulation of GTPase activity by GAP. Mutations in nonessential regions of H-ras p21 as well as mutations in its carboxyl-terminal domain (residues 165-185) and purine binding region (residues 117 and 119) did not decrease the ability of the protein to respond to GAP. In addition, an antibody against the carboxyl-terminal domain did not block GAP activity, supporting the conclusion that GAP does not interact with this region. Transforming mutations at positions 12, 59, and 61 (the phosphoryl binding region) abolished GTPase stimulation by GAP. Point mutations in the putative effector region of ras p21 (amino acids 35, 36, and 38) were also insensitive to GAP. However, a point mutation at position 39, shown previously not to impair effector function, did not alter GAP-p21 interaction. These results indicate that GAP interaction may be essential for ras p21 biological activity and that it may be a ras effector protein.
Use of video shadow for small group interaction awareness on a large interactive display surface (2003)

This paper reports work done as part of the Large Interactive Display Surface(LIDS) project at the University of Waikato. One application of the LIDS equipment is distributed meeting support. In this context large display surfaces are used as shared workspaces by people at collaborating sites. A meeting will start with a shared presentation document, typically an agenda document with summary and detail on agenda items as required. During the meeting, annotations will be made on the shared document, and new pages will be added with notes and drawings.\nTo prevent access collisions and generally mediate use of the shared space, mechanisms to provide awareness of actions of people at other sites are required. In our system a web camera is used to capture a low-resolution image of the person/people near the board on each side. Rather than transmit the image directly we compute a shadow/silhouette The shadow is displayed ‘behind’ other screen content. This provides awareness of position and impending write actions and allows intentional pointing to locations on the screen. It also has the advantages of being transmitted with low bandwidth, being relatively insensitive to low frame rates, and minimizing visual interference with the substantive data being displayed on the screen.
Text-mining the voice of the people (2012)

Statistical techniques help public leaders turn text in unstructured citizen feedback into responsive e-democracy.
Detection of Legionella species in reclaimed water and air with the EnviroAmp Legionella PCR kit and direct fluorescent antibody staining (1995)

Reclaimed water is an important resource for areas with inadequate water supplies. However, there have been few studies on the variety of microorganisms found in this type of water, since typically reclaimed water is examined only for the presence of coliform bacteria. Many microorganisms, including the legionellae, are known to be more resistant to chlorine than are coliform bacteria. Previously, we detected > 10(3) Legionella cells per ml in primary and secondary sewage effluents and observed no significant reduction in population numbers throughout the treatment process. In this study, we detected Legionella spp. in chlorinated effluent by using an EnviroAmp Legionella PCR kit and direct fluorescent antibody (DFA) staining. However, we were not able to isolate Legionella spp. from either natural or seeded reclaimed water samples. This suggests that the Legionella spp. detected by the PCR and DFA methods may be injured or viable but nonculturable after exposure to the high residual chlorine levels typically found in this type of water source. The numbers of coliform bacteria were low (< 2 cells per 100 ml) in most reclaimed water samples and were not correlated with the presence or absence of Legionella spp. We also collected air samples from above a secondary aeration basin and analyzed them by using the PCR, DFA, and plate culture methods. Legionella spp. were detected in the air obtained from above the secondary basin with all three methods. We concluded that the PCR was superior to the culture and DFA methods for detecting Legionella spp. in environmental water samples.
Single Molecules Trapped by Dynamic Inhomogeneous Temperature Fields (2015)

We demonstrate a single molecule trapping concept that modulates the actual driving force of Brownian motion--the temperature. By spatially and temporally varying the temperature at a plasmonic nanostructure, thermodiffusive drifts are induced that are used to trap single nano-objects. A feedback controlled switching of local temperature fields allows us to confine the motion of a single DNA molecule for minutes and tailoring complex effective trapping potentials. This new type of thermophoretic microbeaker even provides control over a well-defined number of single molecules and is scalable to large arrays of trapping structures.
Speed Sensorless Vector Control of a Redundant Permanent Magnet Wind Power Generator (2007)

Permanent magnet motors are coming more and more popular in many low speed and high speed applications. The conventional motor drive, consisting of a standard speed induction motor with a gearbox, can be replaced of a very low speed or a very high speed permanent magnet motor having no gearbox. The advantages are, for example, the increased efficiency and the reliability, and the reduced weight, noise and costs of the whole drive system. Permanent magnet motors can be designed efficiently for very low speeds with a high pole number. This makes PM-machines very attractive for wind power generators, which are in high power (≫ 1 MW) wind mills typically rotating about 10 rpm - 20 rpm. One important aspect of motor drives is the reliability of the drive system. The reliability of the PM wind power generator can be increased by using multiple stator modules or stator segments independent of each other. These segments can be considered, for example, as independent stator parallel windings which are each fed by own frequency converter. If one of the frequency converters fails, the other ones can continue the operation while the failed unit is changed. This paper describes the speed sensorless vector control of a variable speed multi-module PM wind power generator. The theory and laboratory tests are shown for a 4×1 kW PM-generator. Finally, the actual wind power generator tested is a 16 rpm 3 MW axial flux PM-generator consisting of 3 stator modules.
Instrumenting home networks (2011)

In managing and troubleshooting home networks, one of the challenges is in knowing what is actually happening. Availability of a record of events that occurred on the home network before trouble appeared would go a long way toward addressing that challenge. In this position/work-in-progress paper, we consider requirements for a general-purpose logging facility for home networks. Such a facility, if properly designed, would potentially have other uses. We describe several such uses and discuss requirements to be considered in the design of a logging platform that would be widely supported and accepted. We also report on our initial deployment of such a facility.
Monolithic integration of GaN-based micromechanical resonators and HEMTs for timing applications (2012)

A platform for intimate integration of high-frequency gallium nitride (GaN) micromechanical resonators and AlGaN/GaN high electron mobility transistors (HEMTs) is reported. For the first time, cascade of a two-port GaN bulk acoustic resonator and AlGaN/GaN HEMT was co-fabricated on a silicon substrate. A high quality factor (Q) of 7413 is reported for a GaN contour-mode resonator at the resonance frequency of 119.8 MHz. More than 30 dB of signal tuning was achieved by using integrated HEMT for signal readout and amplification at the resonator output.
Myofibrillar ATPase activity during isometric contraction and isomyosin composition in rat single skinned muscle fibres. (1994)

1. Myofibrillar ATPase activity, isometric tension (Po) and unloaded shortening velocity (Vo) were determined in single skinned fibres isolated from rat hindlimb muscles during maximal calcium activation at 12 degrees C. In each fibre, myosin heavy chain (MHC) isoforms were identified using electrophoresis and immunocytochemistry. ATPase activity was determined spectrophotometrically from NADH oxidation in a coupled enzyme assay. 2. On the basis of their MHC isoform composition, the fibres (n = 102) were divided into five groups containing the slow isoform, I MHC, or one of the fast isoforms, IIB MHC, IIA MHC, IIX MHC, or a mixture of the latter three. ATPase activity was significantly higher in IIB than in 2X and IIA fibres (0.230 +/- 0.010, 0.178 +/- 0.023 and 0.168 +/- 0.026 nmol mm-3 s-1, respectively). Mixed fibres had intermediate values. ATPase activity in slow fibres was considerably less (0.045 +/- 0.006 nmol mm-3 s-1). 3. The ratio between ATPase activity and Po, i.e. tension cost, was found to be 2.90 +/- 0.09, 2.56 +/- 0.14, 1.89 +/- 0.22, 1.52 +/- 0.13 and 0.66 +/- 0.004 pmol ATP nM-1 mm-1 s-1 in IIB, mixed, IIX, IIA and slow fibres, respectively. All the differences were statistically significant except that between IIA and IIX fibres. 4. Within each group of fibres with the same MHC composition, ATPase activity was found to correlate with Po, but not Vo. However, ATPase activity was found to correlate with Vo when all the fibre types were pooled together. 5. In thirty-seven fast fibres the MLC ratio, i.e. the proportion of the fast alkali light chain isoform, MLC3f, to the amount of the regulatory light chain, MLC2f, was determined. IIB fibres had the highest proportion of MLC3f and IIA fibres, the lowest. 6. A multiple regression analysis, used to distinguish between the effects of MHC and MLC composition, showed that ATPase activity was insensitive to the MLC ratio, whereas it had a significant impact on Vo. 7. The results obtained in this study indicate that in rat skeletal muscle fibres: (a) ATPase activity during isometric contractions and tension cost are strongly dependent on MHC isoform composition, and (b) there is no evidence that the alkali MLC ratio is a determinant of ATPase activity.
Reactions of poly(phenylene oxide)s with quinones. I. The quinone‐coupling reaction between low‐molecular‐weight poly(2,6‐dimethyl‐1,4‐phenylene oxide) and 3,3′,5,5′‐tetramethyl‐4,4′‐diphenoquinone (1981)

Abstract A bifunctional polymer is formed when low‐molecular‐weight poly(2,6‐dimethyl‐1,4‐phenylene oxide) (I) reacts with 3,3′,5,5′‐tetramethyl‐4,4′‐diphenoquinone (II). Infrared (IR), nuclear magnetic resonance (NMR), and gel permeation chromotography (GPC) measurements indicate that the quinone is bound covalently to the polymer chain as a biphenyl moiety which can be located at a terminal position (III, a = 0) or an internal position (III, a > 0): magnified image Acetylation of III produces a diacetate ester characterized by field desorption mass spectrometry to confirm the bifunctional nature of III. The reaction of I with II proceeds at 25°C but is faster at elevated temperatures or with amine catalysis. Oxidation of III with oxygen and a copper/amine catalyst of the type used initially to prepare I regenerates II from the biphenyl moiety in III in high yield and converts the remaining oxyphenylene units to high‐molecular‐weight polymer.
Improvement of Virtual View Rendering Based on Depth Image (2011)

Virtual view rendering has great importance in 3-D display. In order to get high-quality image of an arbitrary view from 2-D source image, improvement on depth-image based rendering (DIBR) is studied in this paper. By analyzing the process of virtual view rendering of DIBR, an improved method is proposed, which generates new view by modifying parallax of two views and incorporating image in painting. The parallax is deduced from depth information, and modified afterwards according to parallax gradient, so as to reduce hole regions before generating new view by 3-D image warping. To further remove the remaining holes, image in painting combining texture and depth information is incorporated. Experimental results show the superiority of the proposed method.
Municipal Solid Waste Management—an Example of a Partnership Between Five Municipalities (1991)

Throughout the 1970s and 1980s many joint municipal partnerships were established in Denmark to take responsibility for the planning and handling of waste on behalf of municipalities in the partnership. RENO SYD is a typical joint municipal partnership, established to handle the municipal solid waste of five municipalities with a total of 65,000 inhabitants. The paper reviews the by-laws and organization of RENO SYD; the facility financing ownership or leasing; the collection of the various waste types; the reception at recycling cites; the refuse treatment, energy recovery, waste separation, treatment and landfill disposal; recycling; and economics, including tipping fees and taxation.
Efficient aerobic epoxidation of alkenes in perfluorinated solvents catalysed by chiral (salen) Mn complexes (1998)

G. Pozzi and F. Cinato, Chem. Commun., 1998, 877 DOI: 10.1039/A800558C
Neurochemical Characteristics of a Novel Dorsal Root Ganglion X Neuroblastoma Hybrid Cell Line, F‐11 (1987)

We investigated the properties of the novel dorsal root ganglion (DRG) hybrid cell line F-11 to see how closely these cells resembled normal DRG cells. Under normal growth conditions, F-11 cells appeared to contain several short neurite-like processes. However, these cells could also be grown under conditions in which they showed a much more extensive neuronal morphology, exhibiting many long neurites. Several differentiated features of DRG cells were present on F-11 cells. These included the presence of delta-opioid receptors, receptors for prostaglandins and bradykinin, and dihydropyridine-sensitive calcium channels. F-11 cells also synthesized and released a substance P-like compound, as determined by immunoreactivity. Both the number of bradykinin receptors and the voltage-sensitive calcium influx increased on cell differentiation. Opioid agonists (delta-specificity) were found to decrease cyclic AMP levels in F-11 cells in a naloxone- and pertussis toxin-reversible fashion. Bradykinin stimulated the synthesis of inositol-1,4-bisphosphate and inositol-1,4,5-trisphosphate. Ca2+ channel agonists stimulated voltage-sensitive Ca2+ influx in a dose-dependent, stereospecific manner, whereas Ca2+ channel antagonists inhibited Ca2+ influx. F-11 cells should, therefore, prove useful as models for authentic DRG neurons.
Review of Human Immunodeficiency Virus Type 1–Related Opportunistic Infections in Sub‐Saharan Africa (2003)

Understanding the natural history of human immunodeficiency virus type 1 (HIV-1) and opportunistic infections in sub-Saharan Africa is necessary to optimize strategies for the prophylaxis and treatment of opportunistic infections and to understand the likely impact of antiretroviral therapy. We undertook a systematic review of the literature on HIV-1 infection in sub-Saharan Africa to assess data from recent cohorts and selected cross-sectional studies to delineate rates of opportunistic infections, associated CD4 cell counts, and associated mortality. We searched the MEDLINE database and the Cochrane Database of Systematic Reviews and Cochrane Clinical Trials Register for English-language literature published from 1990 through April 2002. Tuberculosis, bacterial infections, and malaria were identified as the leading causes of HIV-related morbidity across sub-Saharan Africa. Of the few studies that reported CD4 cell counts, the range of cell counts at the time of diagnosis of opportunistic infections was wide. Policies regarding the type and timing of opportunistic infection prophylaxis may be region specific and urgently require further study.
Common Subspace for Model and Similarity: Phrase Learning for Caption Generation from Images (2015)

Generating captions to describe images is a fundamental problem that combines computer vision and natural language processing. Recent works focus on descriptive phrases, such as "a white dog" to explain the visual composites of an input image. The phrases can not only express objects, attributes, events, and their relations but can also reduce visual complexity. A caption for an input image can be generated by connecting estimated phrases using a grammar model. However, because phrases are combinations of various words, the number of phrases is much larger than the number of single words. Consequently, the accuracy of phrase estimation suffers from too few training samples per phrase. In this paper, we propose a novel phrase-learning method: Common Subspace for Model and Similarity (CoSMoS). In order to overcome the shortage of training samples, CoSMoS obtains a subspace in which (a) all feature vectors associated with the same phrase are mapped as mutually close, (b) classifiers for each phrase are learned, and (c) training samples are shared among co-occurring phrases. Experimental results demonstrate that our system is more accurate than those in earlier work and that the accuracy increases when the dataset from the web increases.
Proximity Effect Transfer from NbTi into a Semiconductor Heterostructure via Epitaxial Aluminum (2017)

We demonstrate the transfer of the superconducting properties of NbTi, a large-gap high-critical-field superconductor, into an InAs heterostructure via a thin intermediate layer of epitaxial Al. Two device geometries, a Josephson junction and a gate-defined quantum point contact, are used to characterize interface transparency and the two-step proximity effect. In the Josephson junction, multiple Andreev reflections reveal near-unity transparency with an induced gap Δ* = 0.50 meV and a critical temperature of 7.8 K. Tunneling spectroscopy yields a hard induced gap in the InAs adjacent to the superconductor of Δ* = 0.43 meV with substructure characteristic of both Al and NbTi.
<i>In Vitro</i> Selection of Functional Lantipeptides (2012)

In this report we present a method to identify functional artificial lantipeptides. In vitro translation coupled with an enzyme-free protocol for posttranslational modification allows preparation of more than 10(11) different lanthionine containing peptides. This diversity can be searched for functional molecules using mRNA-lantipeptide display. We validated this approach by isolating binders toward Sortase A, a transamidase which is required for virulence of Staphylococcus aureus. The interaction of selected lantipeptides with Sortase A is highly dependent on the presence of a (2S,6R)-lanthionine in the peptide and an active conformation of the protein.
Efficient finite element formulation for geothermal heating systems. Part II: transient (2006)

Abstract This paper presents an extension to the work presented in Part I of this series of two articles to the transient case. Emphasis is placed on the development of a new model for heat flow in a double U‐shape vertical borehole heat exchanger and its thermodynamic interactions with surrounding soil mass. The discretization of the spatial‐temporal domain of the heat pipe model is done by the use of the space–time finite element technique in conjunction with the Petrov–Galerkin method and the finite difference method. The paper shows that the proposed model and the choice of the discretization technique, in addition to the utilization of a sequential numerical algorithm for solving the resulting system of non‐linear equations, have contributed in reducing significantly the required number of finite elements necessary for describing geothermal heating systems. Details of the mathematical derivations and comparison to experimental data are presented. Copyright © 2006 John Wiley & Sons, Ltd.
Real-World Performance of HER2 Testing--National Surgical Adjuvant Breast and Bowel Project Experience (2002)

Trastuzumab (Herceptin) provides clinical benefits for patients diagnosed with advanced breast cancers that have overexpressed the HER2 protein or have amplified the HER2 gene. The National Surgical Adjuvant Breast and Bowel Project (NSABP) Protocol B-31 is designed to test the advantage of adding Herceptin to the adjuvant chemotherapeutic regimen of doxorubicin and cyclophosphamide followed by paclitaxel (Taxol) in the treatment of stage II breast cancer with HER2 overexpression or gene amplification. Eligibility is based on HER2 assay results submitted by the accruing institutions. We conducted a central review of the first 104 cases entered in this trial on the basis of immunohistochemistry (IHC) results. We found that 18% of the community-based assays, which were used to establish the eligibility of patients to participate in the B-31 study, could not be confirmed by HercepTest IHC or fluorescence in situ hybridization (FISH) by a central testing facility. This report provides a snapshot of the quality of HER2 assays performed in laboratories nationwide.
Inflammatory Cytokines Induce Expression of Chemokines by Human Retinal Cells: Role in Chemokine Receptor Mediated Age-related Macular Degeneration (2015)

Chemokine reeptor-3 (CCR-3) was shown to be associated with choroidal neovascularization (CNV) in age-related macular degeneration (AMD). AMD is a vision threatening retinal disease that affects the aging population world-wide. Retinal pigment epithelium and choroid in the posterior part of the retina are the key tissues targeted in the pathogenesis of CNV in AMD. We used human retinal pigment epithelial (HRPE) and choroidal fibroblast (HCHF) cells, prepared from aged adult human donor eyes, to evaluate the expression of major CCR-3 ligands, CCL-5, CCL -7, CCL-11,CCL-24 and CCL-26. Microarray analysis of gene expression in HRPE cells treated with inflammatory cytokine mix (ICM= IFN-γ+TNF-α+IL-1β) revealed 75 and 23-fold increase in CCL-5 and CCL-7 respectively, but not CCL-11, CCL-24 and CCL-26. Chemokine secretion studies of the production of CCL5 and CCL7 by HRPE corroborated with the gene expression analysis data. When the HRPE cells were treated with either individual cytokines or the ICM, both CCL-5 and CCL-7 were produced in a dose dependent manner. Similar to the gene expression data, the ICM did not enhance HRPE production of CCL-11, CCL-24 and CCL-26. CCL-11 and CCL-26 were increased with IL-4 treatment and this HRPE production was augmented in the presence of TNF-α and IL1β. When HCHF cells were treated with either individual cytokines or the ICM, both CCL-5 and CCL-7 were produced in a dose dependent fashion. IL-4 induced low levels of CCL-11 and CCL-26 in HCHF and this production was significantly enhanced by TNF-α. Under these conditions, neither HRPE nor HCHF were demonstrated to produce CCL-24. These data demonstrate that chronic inflammation triggers CCL-5 and CCL-7 release by HRPE and HCHF and the subsequent interactions with CCR3 may participate in pathologic processes in AMD.