gebhart/patrepeval huggingface dataset:
Influence of Enol Ether Amino Acids, Inhibitors of Ethylene Biosynthesis, on Aminoacyl Transfer RNA Synthetases and Protein Synthesis (1979)

The analogs of rhizobitoxine, aminoethoxyvinylglycine (AVG) (l-2-amino-4-2'-aminoethoxy-trans-3 butenoic acid) and methoxyvinylglycine (MVG) (l-2-amino-4-methoxy-trans-3-butenoic acid), that are potent inhibitors of ethylene biosynthesis at 0.1 millimolar also inhibited protein synthesis and charging of tRNA especially at 1 millimolar and higher concentrations. The saturated analog of MVG inhibited ethylene synthesis while the saturated analog of AVG did not. Both saturated AVG and MVG inhibit methionyl- and leucyl-amino acyl-tRNA synthetase. Because of the inhibition of amino acid metabolism in plant tissues by these rhizobitoxine analogs caution is advised in interpreting the results obtained with concentrations of compounds above 0.1 millimolar.
Conversion of crude oil to methane by a microbial consortium enriched from oil reservoir production waters (2014)

The methanogenic biodegradation of crude oil is an important process occurring in petroleum reservoirs and other oil-containing environments such as contaminated aquifers. In this process, syntrophic bacteria degrade hydrocarbon substrates to products such as acetate, and/or H2 and CO2 that are then used by methanogens to produce methane in a thermodynamically dependent manner. We enriched a methanogenic crude oil-degrading consortium from production waters sampled from a low temperature heavy oil reservoir. Alkylsuccinates indicative of fumarate addition to C5 and C6 n-alkanes were identified in the culture (above levels found in controls), corresponding to the detection of an alkyl succinate synthase encoding gene (assA/masA) in the culture. In addition, the enrichment culture was tested for its ability to produce methane from residual oil in a sandstone-packed column system simulating a mature field. Methane production rates of up to 5.8 μmol CH4/g of oil/day were measured in the column system. Amounts of produced methane were in relatively good agreement with hydrocarbon loss showing depletion of more than 50% of saturate and aromatic hydrocarbons. Microbial community analysis revealed that the enrichment culture was dominated by members of the genus Smithella, Methanosaeta, and Methanoculleus. However, a shift in microbial community occurred following incubation of the enrichment in the sandstone columns. Here, Methanobacterium sp. were most abundant, as were bacterial members of the genus Pseudomonas and other known biofilm forming organisms. Our findings show that microorganisms enriched from petroleum reservoir waters can bioconvert crude oil components to methane both planktonically and in sandstone-packed columns as test systems. Further, the results suggest that different organisms may contribute to oil biodegradation within different phases (e.g., planktonic vs. sessile) within a subsurface crude oil reservoir.
The compliance characteristics of the Portex Soft-Seal cuff improves seal against leakage of fluid in a pig trachea model (1999)

Crit Care 1999, 3 3 ( (s su up pp pl l 1 1) ):P1 I In nt tr ro od du uc ct ti io on n: : Critically ill patients requiring intensive care are at risk of iatrogenic ocular damage. Studies have reported an incidence of eye problems of up to 40% in critically ill ventilated patients. We conducted this study to assess the incidence of ocular complications in our intensive care unit where all patients are cared for according to an eye care standard.
Practical aspects of the doppler effect in mobile communication systems (2008)

The purpose of this paper is to conduct theoretical analysis and calculation of transmitter motion influence on received signal parameters. The form of the straight electric field and the Doppler frequency is the result of the theoretical analysis presented in this paper. To solve of the wave equation we have made use of integral transformations i.e. the Laplace transform (in relation to normalized time variable) and the Fourier transform (in relation to space variables). Finally, the practical time aspects of the location signal source measurement on the basis of the frequency Doppler is presented.
Design of a Multicore Sparse Cholesky Factorization Using DAGs (2010)

The rapid emergence of multicore machines has led to the need to design new algorithms that are efficient on these architectures. Here, we consider the solution of sparse symmetric positive-definite linear systems by Cholesky factorization. We were motivated by the successful division of the computation in the dense case into tasks on blocks and use of a task manager to exploit all the parallelism that is available between these tasks, whose dependencies may be represented by a directed acyclic graph (DAG). Our sparse algorithm is built on the assembly tree and subdivides the work at each node into tasks on blocks of the Cholesky factor. The dependencies between these tasks may again be represented by a DAG. To limit memory requirements, blocks are updated directly rather than through generated-element matrices. Our algorithm is implemented within a new efficient and portable solver HSL_MA87. It is written in Fortran 95 plus OpenMP and is available as part of the software library HSL. Using problems arising from a range of applications, we present experimental results that support our design choices and demonstrate that HSL_MA87 obtains good serial and parallel times on our 8-core test machines. Comparisons are made with existing modern solvers and show that HSL_MA87 performs well, particularly in the case of very large problems.
Direct Cast Titanium Aluminide Strip (1991)

Abstract : The purpose of the Phase I research project is to demonstrate the feasibility of melting titanium alloys using Duriron's induction slag melting process and casting them into rapidly solidified strip by RIBTEC's melt overflow process. The strip might be used for SiC-Ti metal matrix composites for advanced airframe structures.
Boron Isotope Separation by Ion Exchange Chromatography Using Weakly Basic Anion Exchange Resin (1980)

Abstract Isotopic plateau displacement chromatography, a useful method for isotope separation is presented. The boric acid band formed in a column of weakly basic anion exchange resin Diaion WA21 can be eluted with pure water. In order to obtain good accumulation of the isotope effect, a series of experiments with different migration length were carried out. The boron-10 enriched part of the boric acid absorbed band was always preceded by the isotopic plateau part, in which the atomic fraction of boron-10 was maintained at its original value. The atomic fraction of boron-10 at the end of the chromatogram increased with migration length, and in the case of 256-m migration, boron-10 was enriched from its original atomic fraction of 19.84 to 91.00%, the separation factor S being constant irrespective of migration length: S=1.0100±0.0005.
Novel Regulation of Heat Shock Genes during Carrot Somatic Embryo Development (1989)

J. Lynn Zimmerman, Nestor Apuya, Kamel Darwish, Cynthia O'Carroll, Novel Regulation of Heat Shock Genes during Carrot Somatic Embryo Development, The Plant Cell, Vol. 1, No. 12 (Dec., 1989), pp. 1137-1146
Racemic Synthesis and Antiviral Evaluation of 4′(α)-Hydroxymethyl and 6′(α)-Methyl Substituted Apiosyl Nucleosides (2007)

This article reports the novel synthesis of substituted apiosyl nucleosides. The key apiosyl intermediate 9 was constructed by sequential ozonolysis, reductions, and acetylation from the ester derivative 6. The nucleosides of uracil, thymine, cytosine, and adenine were synthesized using the glycosyl condensation procedure (silyated base and TMSOTf). The antiviral activities of the synthesized compounds against the HIV-1, HSV-1, HSV-2, and HCMV viruses were evaluated. The adenine derivative 26 showed weak anti-HIV activity (EC(50) = 10.1 microg/ml) without exhibiting any cytotoxicity up to a concentration of 100 microM.
STRUCTURAL STUDIES BY ELECTRON TOMOGRAPHY: From Cells to Molecules (2005)

Electron tomography (ET) is uniquely suited to obtain three-dimensional reconstructions of pleomorphic structures, such as cells, organelles or supramolecular assemblies. Although the principles of ET have been known for decades, its use has gathered momentum only in recent years, thanks to technological advances and its combination with improved specimen preparation techniques. The rapid freezing/freeze-substitution preparation is applicable to whole cells and tissues, and it is the method of choice for ET investigations of cellular ultrastructure. The frozen-hydrated preparation provides the best possible structural preservation and allows the imaging of molecules, complexes, and supramolecular assemblies in their native state and their natural environment. Devoid of staining and chemical fixation artifacts, cryo-ET provides a faithful representation of both the surface and internal structure of molecules. In combination with advanced computational methods, such as molecular identification based on pattern recognition techniques, cryo-ET is currently the most promising approach to comprehensively map macromolecular architecture inside cellular tomograms.
Submicron trackwidth and stripe height MR sensor test structures (1996)

Magnetic recording areal densities greater than 5 Gbit/in/sup 2/ will require magnetoresistive (MR) sensors with critical dimensions below 1.0 /spl mu/m. The longitudinal bias scheme used for this sensor size must provide stable device operation and must be compatible with fine dimension lithographic processing. A contiguous junction sensor structure with longitudinal material abutted to the sensor magnetic films and with lead material self aligned to the longitudinal bias material can satisfy these requirements. This is demonstrated by the fabrication and testing of submicron unshielded MR structures with stable transfer curves.
Single-cell perforin and granzyme expression reveals the anatomical localization of effector CD8<sup>+</sup>T cells in influenza virus-infected mice (2003)

Influenza virus infection activates cytolytic T lymphocytes (CTL) that contribute to viral clearance by releasing perforin and granzymes from cytoplasmic granules. Virus-specific, perforin-dependent CD8(+) CTL were detected in freshly isolated cells from the mouse lung parenchyma but not from the mediastinal lymph nodes (MLN), where they are primed, or from the spleen during primary influenza virus infection. To determine whether this difference was due to the low frequency or incomplete maturation of effector CTL in MLN, we measured expression of perforin, granzymes A, B, and C, and IFN-gamma mRNAs in CD8(+) populations and single cells immediately after isolation from virus-infected mice. Quantitative PCR revealed significant expression of perforin, granzyme A, granzyme B, and IFN-gamma in activated CD8(+) cells from MLN, spleen, and lung parenchyma. Granzyme C expression was not detected. Individual activated or nucleoprotein peptide/class I tetramer-binding CD8(+) cells from the three tissues expressed diverse combinations of perforin, granzyme, and IFN-gamma mRNAs. Although cells from lung expressed granzymes A and B at higher frequency, each of the tissues contained cells that coexpressed perforin with granzymes A and/or B. The main difference between MLN and lung was the elevated frequency of activated CD8(+) T cells in the lung, rather than their perforin/granzyme expression profile. The data suggest that some CTL mature into perforin/granzyme-expressing effector cells in MLN but reach detectable frequencies only when they accumulate in the infected lung.
Amentoflavone Induces Anti-angiogenic and Anti-metastatic Effects Through Suppression of NF-κB Activation in MCF-7 cells. (2015)

The aim of the present study was to investigate whether amentoflavone induces anti-angiogenic and anti-metastatic effects through suppression of NF-κB activation in breast cancer in vitro. Effects of NF-κB inhibitor 4-N-[2-(4-phenoxyphenyl) ethyl] quinazoline-4, 6-diamine (QNZ) and amentoflavone on the expression and secretion of angiogenesis- and metastasis-related proteins and cell invasion were investigated by western blotting, enzyme-linked immunosorbent assay (ELISA), and invasion assays. We also verified the effects of QNZ and amentoflavone on lipopolysaccharides (LPS)-activated cell invasion. Obtained results indicated that both QNZ and amentoflavone reduce NF-κB activation, expression and secretion of angiogenesis- and metastasis-related proteins, and cell invasion. QNZ and amentoflavone also reverse LPS-activated cell invasion. In conclusion, inhibition of NF-κB activation decreases expression and secretion of angiogenesis- and metastasis-related proteins. Amentoflavone may induce anti-angiogenic and anti-metastatic effects through suppression of NF-κB activation.
Hypoglycemic effects of the n-Butanol part of Sorbus tianschanica. (2014)

[Objective]To study the hypoglycemic effect of n-butanol part of tianschanica.[Method]Sorbus tianshanica was extracted with flux method,through gradient elution,n-butanol part was obtained.The diabetic rat model was induced by intraperitoneal injection of STZ,the rat blood glucose levels was detected.[Result]n-butanol part could lower blood glucose level in STZ induced hyperglycaemic rats.[Conclusion] Sorbus tianschanica has significant hypoglycemic effect.
Financial Exclusion : A New Angle to Urban Poverty in Latin America (2005)

Data gathered during studies conducted over the past four years in Mexico City, Mexico (Distrito Federal); Bogota, Colombia, and in several Brazilian cities suggests that in these countries somewhere between 65 and 85 percent of households are “unbanked”, those who do not hold any kind of deposit or transaction account in any formal sector financial institution.1 As Figure 1 shows, the proportions in developed countries are dramatically different. Not surprisingly, the unbanked in all countries show other characteristics of marginality – low incomes, low levels of education, and, in developed countries, high rates among minority and immigrant populations.
Carborane polymers (1972)

The evolution of polymers incorporating the closo-carboranes C 2 B 5 H 7 and C 2 B 10 H 12 is reviewed. Both addition and condensation polymers and Copolymers are described which include various examples ofpolyesters, polyvinyls and polyamides. Primary emphasis is placed upon the polycarboranesiloxane polymers which exhibit unparalleled thermal stability.
An endo-acting proline-specific oligopeptidase from Treponema denticola ATCC 35405: evidence of hydrolysis of human bioactive peptides (1994)

An endo-acting proline-specific oligopeptidase (prolyl oligopeptidase [POPase], EC 3.4.21.26) was purified to homogeneity from the Triton X-100 extracts of cells of Treponema denticola ATCC 35405 (a human oral spirochete) by a procedure that comprised five successive fast protein liquid chromatography steps. The POPase is a cell-associated 75- to 77-kDa protein with an isoelectric point of ca. 6.5. The enzyme hydrolyzed (optimum pH 6.5) the Pro-pNA bond in carbobenzoxy-Gly-Pro-p-nitroanilide (Z-Gly-Pro-pNA) and bonds at the carboxyl side of proline in several human bioactive peptides, such as bradykinin, substance P, neurotensin, angiotensins, oxytocin, vasopressin, and human endothelin fragment 22-38. The minimum hydrolyzable peptide size was tetrapeptide P3P2P1P'1, while the maximum substrate size was ca. 3 kDa. An imino acid residue in position P1 was absolutely necessary. The hydrolysis of Z-Gly-Pro-pNA was potently inhibited by the following, with the Ki(app) (in micromolar) in parentheses: insulin B-chain (0.7), human endothelin-1 (0.5), neuropeptide Y (1.7), substance P (32.0), T-kinin (4.0), neurotensin (5.0), and bradykinin (16.0). Chemical modification and inhibition studies suggest that the POPase is a serine endopeptidase whose activity depends on the catalytic triad of COOH ... Ser ... His but not on a metal. The amino acid sequence around the putative active-site serine is Gly-Gly-Ser-Asn-Pro-Gly. The enzyme is suggested to contain a reactive cysteinyl residue near the active site. Amino acid residues 4 to 24 of the first 24 N-terminal residues showed a homology of 71% with the POPase precursor from Flavobacterium meningosepticum and considerable homology with the Aeromonas hydrophila POPase. The ready hydrolysis of human bioactive peptides at bonds involving an imino acid residue suggests that enzymes like POPase may contribute to the chronicity of periodontal infections by participating in the peptidolytic processing of those peptides.
Closing the Distance (1998)

Geriatric technicians work in the field, providing a link between care providers and rural elderly patients. In this study, the new role enhanced patient outcomes and satisfaction.
<i>Pseudomonas aeruginosa</i> Vaccine in Cancer Patients (1973)

During 1969 to 1972, 361 adult patients (176 vaccinees, 185 control patients) at a cancer center were randomized into a prospective study of the lipopolysaccharide vaccine derived from 7 serotypes ofPseudomonas aeruginosa. There were no significant differences in underlying disease, antibiotic therapy, or antineoplastic treatment between the vaccine and control groups. Fatal bacteremic pseudomonas infection occurred in 19 control subjects as opposed to in 10 vaccinees, and nonbacteremic pseudomonas infections associated with death occurred in 12 control patients and in 3 vaccinees. Protection against bacteremic death was not significant, but the mortality rate associated with pseudomonas infection was 13 out of 176 for the vaccinated group versus 31 out of 185 in the control group (P< 0.01). Fatal pseudomonas infections in both vaccine and control groups were associated with marked leukopenia, low titers of opsonizing pseudomonas antibodies, or both. As an adjunct to therapy of neoplasms, immunoprophylaxis with pseudomonas vaccine was associated with a significant but limited reduction in fatal pseudomonas infection. Vaccine administration was also accompanied by a high incidence of untoward reactions.
Aurora 3d-measurement from whole-sky time series image using fish-eye stereo camera (2016)

Three-dimensional analysis of aurora is important for the research of solar wind and magnetic storms, because aurora reflects the relationship between solar wind and terrestrial magnetism. Therefore a method to reconstruct a three-dimensional shape of aurora precisely is demanded. In this paper, a method to measure three-dimensional shape of auroa by using only two fish-eye cameras is proposed. Two fish-eye cameras were installed at Fairbanks, Alaska, U.S.A to get the time series images of aurora. The images photographed by the cameras are performed calibration by using the star to estimate the camera attitude. The dense feature points are detected from the images of aurora by using template matching though the image of aurora has few characteristic pattern. Moreover, a feature point tracking between two images continuing in time is performed to improve precision of the feature point detection. Then the three-dimensional coordinates of feature points are calculated by triangulation, aurora shape is measured and visualized.
Robotic Transperitoneal Detrusor Myotomy: Description of a Novel Technique (2005)

The da Vinci Surgical Robotic System has recently been added to the armamentarium of minimally invasive surgeon and has been shown to be useful to urologists in performing complex operations. We report the first case of detrusor myotomy performed using the da Vinci, describe the novel technique, and review the indications and published outcomes of detrusor myotomy to identify potential applications of this novel technique in patients with neurogenic bladder.
Induced hyperactivity in boar spermatozoa and its evaluation by computer-assisted sperm analysis (2004)

Hyperactivity, a form of sperm motility characterized by vigorous flagellar movements, has been proposed as essential for fertilization in mammals. The objective of the present study was to establish a method for inducing hyperactivity in vitro in boar spermatozoa and to define threshold values to differentiate between hyperactive and non-hyperactive spermatozoa by computer-assisted sperm analysis (CASA) as a prerequisite for analyzing the energy metabolism during hyperactivity. In TALP-HEPES medium, non-frozen boar spermatozoa were stimulated to hyperactivity by 50 micromol l(-1) Ca2+ within 15 min at 37 degrees C if 5 micromol l(-1) of the Ca2+ ionophore A23187 was present. If 25% seminal plasma was present, boar spermatozoa required higher Ca2+ concentrations (about 700 micromol l(-1)) for hyperactivity. Under both conditions, immobilization and head-to-head agglutination were low so that hyperactive spermatozoa could be analyzed for at least 40 min. The transition from normal to hyperactive movement was characterized by an increase in flagellar beat angle from 49 degrees +/- 12 degrees to 200 degrees +/- 36 degrees (n = 32) and a decrease in flagellar curvature ratio from 0.89 +/- 0.04 to 0.47 +/- 0.11 (n = 32). For quantification of hyperactive boar sperm, kinematic parameters of hyperactive and non-hyperactive spermatozoa were measured by CASA and statistically evaluated (receiver operating characteristic (ROC) curve analysis). The threshold values of the following four parameters were well suited for differentiating between hyperactive and non-hyperactive boar spermatozoa (ROC curve analysis: > 50% specificity at 100% sensitivity). Hyperactive boar spermatozoa showed mean lateral head displacement > 3.5 microm, curvilinear velocity > 97 microm s(-1), linearity < 32% and wobble < 71%. According to this multiparametric definition, induction of hyperactivity increased significantly (P < 0.0001) the fraction of hyperactive spermatozoa in semen samples from 5.1 +/- 4.3% (n = 13) to 48.3 +/- 6.6% (n = 7) in the absence and to 44.2 +/- 7.6% (n = 10) in the presence of 25% seminal plasma, while the overall percentage of motile spermatozoa did not change significantly.
Id proteins are dynamically expressed in normal epidermis and dysregulated in squamous cell carcinoma. (2000)

Helix-loop-helix genes regulate many developmental pathways, and growing evidence associates dysregulated expression with tumorigenesis. We observed Id-1, Id-2, and Id-3 mRNA expression in proliferating human keratinocytes in vitro with subsequent down-regulation with differentiation. Immunohistochemical analysis of human tissue sections identified cytoplasmic Id-1 expression and nuclear Id-2 and Id-3 expression in the proliferating layers of the epidermis. Furthermore, we observed a columnar pattern of Id-2 and Id-3 staining, which may relate to the epidermal proliferative unit. In squamous cell carcinoma of the head and neck, Id protein immunoreactivity was observed in the majority of malignant keratinocytes in the most poorly differentiated sections, with reduced staining in well-differentiated disease.