gebhart/patrepeval huggingface dataset:
Lysine 58 and Histidine 66 at the C-terminal α-Helix of Monocyte Chemoattractant Protein-1 Are Essential for Glycosaminoglycan Binding (1998)

Monocytes rolling on the endothelial cell layer interact with monocyte chemoattractant protein-1 (MCP-1) that is tethered to the proteoglycans on the luminal side of the endothelial cells and consequently initiate adhesion of monocytes in the early phase of immune response. The amino acid residues in MCP-1 involved in tethering to the proteoglycans have not been elucidated. MCP-1 showed binding to [3H]heparin with a KD of 1.5 microM. We substituted lysine or histidine residues at the C-terminal end of MCP-1 with alanine residues and tested these mutants for their ability to bind heparin, heparan sulfate, hyaluronic acid, and chondroitin sulfate-C. Substitution of Lys-58 or His-66 drastically reduced glycosaminoglycan binding. Substitution of Lys-56 or deletion of the five amino acid residues at the C terminus, including Lys-75, did not alter the heparin binding ability, suggesting that the other lysine residues at the C terminus are not involved in glycosaminoglycan binding. MCP-1 and its mutants did not bind hyaluronic acid as strongly as the other subunits of the GAGs. Substitution of Lys-58 or His-66 by alanine that prevented glycosaminoglycan binding did not affect Ca2+ influx, receptor binding, or chemotactic activity elicited by the chemokine on monocytic THP-1 cells. Therefore, we conclude that the Lys-58 and His-66 residues in the C-terminal alpha-helix of MCP-1 are essential for glycosaminoglycan binding and probably for the binding to the endothelial surface proteoglycans.
Heparin and non‐heparin‐like dextrans differentially modulate endothelial cell proliferation: <i>In vitro</i> evaluation with soluble and crosslinked polysaccharide matrices (2002)

Proliferation of endothelial cells (ECs) is a cellular step of particular importance for implanted cardiovascular biomaterials. Heparin and some synthetic water-soluble non-anticoagulant polysaccharides derived from dextran and bearing anionic carboxymethyl and hydrophobic benzylamine groups were first investigated for their effects on EC proliferation in vitro. The results assessed by cell counting, 3H-thymidine uptake, and flow cytometry analysis, showed that the derivatized dextran-bearing hydrophobic groups stimulated the EC growth in the presence of serum, whereas native dextran or dextran-bearing anionic carboxymethyl groups were inactive and heparin was slightly inhibitory. Then, we showed that the derivatized dextran enhanced EC proliferation by potentiation of the mitogenic activities of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2), two potent EC growth factors. In the presence of 2 nM of derivatized dextran, a 3-fold and 13-fold increase of 3H-thymidine uptake was obtained with VEGF and FGF-2, respectively. Finally, proliferation of ECs was investigated on crosslinked gels made of polysaccharides. It is of interest that EC proliferation was higher on gels containing the derivatized dextran than on plain hydrogels, and heparinized gels inhibited cell proliferation. From the obtained results, we propose that the synthetic non-heparin-like dextran may be of interest as a coating for the endothelialization of cardiovascular biomaterials.
Al x Ga 1−x As native-oxide-based distributed Bragg reflectors for vertical cavity surface emitting lasers (1998)

The problems associated with constructing AlxGa1−xAs native-oxide-based distributed Bragg reflectors (DBRs) for vertical cavity surface emitting lasers are investigated. Reflection and stability measurements are performed on structures with central λ/2 cavities (λ∼980 nm) of GaAs surrounded by two periods of native-oxide-based DBRs on the top and 2.5 periods on the bottom. Prior to crystal oxidation (H2O vapor+N2, 430 °C) a period of the DBRs consists of a ∼λ/4 optically thick layer of GaAs and a thicker (oxidation) layer of AlxGa1−xAs (x=0.95, 0.96, 0.97, 0.98, 1.00) surrounded by thinner (∼100 Å) buffer layers that are AlyGa1−yAs (y=0, 0.25, 0.50, 0.65, 0.070, 0.75, 0.80, 0.85). The DBRs are formed after oxidation of the high Al composition AlxGa1−xAs layers, and to some extent the AlyGa1−yAs buffer layers, forming a ∼λ/4 optically thick layer of the native oxide. For comparison, more complicated DBRs are created by oxidizing superlattice layers. It is found that the AlxGa1−xAs composition, x, of the oxidation layer, choice of oxidizing or nonoxidizing AlyGa1−yAs buffer layers (y), oxidation parameters, and post-processing parameters determine the DBR quality and stability, as well as the possibility of reoxidation.
Implants, Mechanical Devices, and Vascular Surgery for Erectile Dysfunction (2010)

It is important for the future of the field that patients be made aware of all treatment options for erectile dysfunction in order to make an informed decision. The treating physician should be aware of the patient's medical and sexual history in helping to guide the decision. More research is needed in the area of revascularization surgery, in particular, venous outflow surgery.
Diverse Sequential Subset Selection for Supervised Video Summarization (2014)

Video summarization is a challenging problem with great application potential. Whereas prior approaches, largely unsupervised in nature, focus on sampling use-ful frames and assembling them as summaries, we consider video summarization as a supervised subset selection problem. Our idea is to teach the system to learn from human-created summaries how to select informative and diverse subsets, so as to best meet evaluation metrics derived from human-perceived quality. To this end, we propose the sequential determinantal point process (seqDPP), a proba-bilistic model for diverse sequential subset selection. Our novel seqDPP heeds the inherent sequential structures in video data, thus overcoming the deficiency of the standard DPP, which treats video frames as randomly permutable items. Mean-while, seqDPP retains the power of modeling diverse subsets, essential for summa-rization. Our extensive results of summarizing videos from 3 datasets demonstrate the superior performance of our method, compared to not only existing unsuper-vised methods but also naive applications of the standard DPP model. 1
CD147 monoclonal antibodies induce homotypic cell aggregation of monocytic cell line U937 via LFA‐1/ICAM‐1 pathway (1999)

CD147 is a 50 000-60 000 MW glycoprotein of the immunoglobulin superfamily broadly expressed on haemopoietic cell lines and peripheral blood cells. In the present study, six monoclonal antibodies (mAbs) directed against the CD147 protein were generated. The antigen defined by the generated CD147 mAbs is widely expressed on haemopoietic cell lines, peripheral blood cells and is a lymphocyte activation-associated cell surface molecule. The generated CD147 mAbs precipitated a broad protein band from U937 cells of 45 000-65 000 MW under reducing conditions. Functional analysis indicated that the CD147 mAbs markedly induced homotypic cell aggregation of U937 cells, but not K562 cells. The CD147 mAb-induced cell aggregation was inhibited by leucocyte function-antigen-1 (LFA-1) and intercellular adhesion molecule-1 (ICAM-1) mAbs. However, the expression of LFA-1 and ICAM-1 molecules on U937 was not altered by CD147 mAb treatment. The U937 cell aggregation induced by CD147 mAb was also inhibited by ethylenediamine tetra-acetic acid (EDTA), sodium azide and when incubated at 4 degrees. We therefore propose that the binding of CD147 mAb to CD147 molecule, which mimics the natural ligand binding, may generate intracellular signals that activate LFA-1/ICAM-1 intercellular adhesion pathway.
Performance analysis and design of punctured turbo codes (2006)

This thesis was digitised by the British Library from microfilm. You can acquire a single copy of this thesis for research purposes by clicking on the padlock icon on the thesis file. Please be aware that the text in the supplied thesis pdf file may not be as clear as text in a thesis that was born digital or digitised directly from paper due to the conversion in format. However, all of the theses in Apollo that were digitised from microfilm are readable and have been processed by optical character recognition (OCR) technology which means the reader can search and find text within the document. If you are the author of this thesis and would like to make your work openly available, please contact us: thesis@repository.cam.ac.uk
Advertiments de les conseqüències civils i fiscals dels canvis de veïnatge civil (2013)

La seccio de Dret i la seccio de Dret fiscal i financer de l'ICAB van organitzar la conferencia Advertiments sobre les consequencies civils i fiscals dels canvis de veinatge civil a carrec d'Alejandro Ebrat i Meritxell Gabarro, qui fa el resum de les questions mes destacades que s'hi van tractar.
α-Catenin Is a Tumor Suppressor That Controls Cell Accumulation by Regulating the Localization and Activity of the Transcriptional Coactivator Yap1 (2011)

The Hippo pathway regulates contact inhibition of cell proliferation and, ultimately, organ size in diverse multicellular organisms. Inactivation of the Hippo pathway promotes nuclear localization of the transcriptional coactivator Yap1, a Hippo pathway effector, and can cause cancer. Here, we show that deletion of αE (α epithelial) catenin in the hair follicle stem cell compartment resulted in the development of skin squamous cell carcinoma in mice. Tumor formation was accelerated by simultaneous deletion of αE-catenin and the tumor suppressor-encoding gene p53. A small interfering RNA screen revealed a functional connection between αE-catenin and Yap1. By interacting with Yap1, αE-catenin promoted its cytoplasmic localization, and Yap1 showed constitutive nuclear localization in αE-catenin-null cells. We also found an inverse correlation between αE-catenin abundance and Yap1 activation in human squamous cell carcinoma tumors. These findings identify αE-catenin as a tumor suppressor that inhibits Yap1 activity and sequesters it in the cytoplasm.
The seismic electric effect (1936)

Abstract The seismic electric effect is the name which has been given to the variation of earth resistivity with elastic deformation. An account is given of the use of this effect in recording seismic waves. The recording circuit employs a direct current sent through the ground by means of a storage battery and spaced electrodes, and the changes in current due to seismic disturbances are recorded. An analysis of the noise appearing in the recording circuit is given and methods of reducing the noise level are described. The results of measurements on the magnitude of the effect are given, and the sensitivity obtainable with the seismic electric method is compared with that given by an electromagnetic pickup.
The Transcriptional Regulators of the CRP Family Regulate Different Essential Bacterial Functions and Can Be Inherited Vertically and Horizontally (2017)

One of the best-studied transcriptional regulatory proteins in bacteria is the <i>Escherichia coli</i> catabolite repressor protein (CRP) that when complexed with 3'-5'-cyclic AMP (cAMP) changes its conformation and interacts with specific DNA-sequences. CRP DNA-binding can result in positive or negative regulation of gene expression depending on the position of its interaction with respect to RNA polymerase binding site. The aim of this work is to review the biological role and phylogenetic relations that some members of the CRP family of transcriptional regulators (also known as cAMP receptor protein family) have in different bacterial species. This work is not intended to give an exhaustive revision of bacterial CRP-orthologs, but to provide examples of the role that these proteins play in the expression of genes that are fundamental for the life style of some bacterial species. We highlight the conservation of their structural characteristics and of their binding to conserved-DNA sequences, in contrast to their very diverse repertoire of gene activation. CRP activates a wide variety of fundamental genes for the biological characteristic of each bacterial species, which in several instances form part of their core-genome (defined as the gene sequences present in all members of a bacterial species). We present evidence that support the fact that some of the transcriptional regulators that belong to the CRP family in different bacterial species, and some of the genes that are regulated by them, can be inherited by horizontal gene transfer. These data are discussed in the framework of bacterial evolution models.
Synthetic Lipid Bilayer Membranes (1969)

The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
Ultrasonic phased array sensor for electric travel aids for visually impaired people (2007)

An ultrasonic phased array sensor system is developed. The system has high capability on azimuthal resolution and plural obstacles detection, and is considered as a sensing system of an electric travel aids for visually impaired people. The developed phased array consists of multiple sound tubes to avoid the grating lobe problem even if one uses large size of ultrasonic sensor elements. To measure plural obstacles in different direction at one time, we applied a method that uses coded transmitting pulse trains to the phased array transmitter. The method reduces the interference between ultrasonic pulses sent out to different directions, and realizes the simultaneous measurement in multiple directions. Experimental results also shown to verify the performance of the phased array.
Cyclin A2 Induces Cardiac Regeneration After Myocardial Infarction Through Cytokinesis of Adult Cardiomyocytes (2014)

Cyclin A2 (Ccna2), normally silenced after birth in the mammalian heart, can induce cardiac repair in small-animal models of myocardial infarction. We report that delivery of the Ccna2 gene to infarcted porcine hearts invokes a regenerative response. We used a catheter-based approach to occlude the left anterior descending artery in swine, which resulted in substantial myocardial infarction. A week later, we performed left lateral thoracotomy and injected adenovirus carrying complementary DNA encoding CCNA2 or null adenovirus into peri-infarct myocardium. Six weeks after treatment, we assessed cardiac contractile function using multimodality imaging including magnetic resonance imaging, which demonstrated ~18% increase in ejection fraction of Ccna2-treated pigs and ~4% decrease in control pigs. Histologic studies demonstrate in vivo evidence of increased cardiomyocyte mitoses, increased cardiomyocyte number, and decreased fibrosis in the experimental pigs. Using time-lapse microscopic imaging of cultured adult porcine cardiomyocytes, we also show that Ccna2 elicits cytokinesis of adult porcine cardiomyocytes with preservation of sarcomeric structure. These data provide a compelling framework for the design and development of cardiac regenerative therapies based on cardiomyocyte cell cycle regulation.
Did You Say U2 or YouTube? (2016)

Web search via voice is becoming increasingly popular, taking advantage of recent advances in automatic speech recognition. Speech recognition systems are trained using audio transcripts, which can be generated by a paid annotator listening to some audio and manually transcribing it. This paper considers an alternative source of training data for speech recognition, called implicit transcription. This is based on Web search clicks and reformulations, which can be interpreted as validating or correcting the recognition done during a real Web search. This can give a large amount of free training data that matches the exact characteristics of real incoming voice searches and the implicit transcriptions can better reflect the needs of real users because they come from the user who generated the audio. On an overall basis we demonstrate that the new training data has value in improving speech recognition. We further show that the in-context feedback from real users can allow the speech recognizer to exploit contextual signals, and reduce the recognition error rate further by up to 23%.
1-Alkyl- (or aryl-) amino-2-methylpropane-2-thiols. Bi- and tetradentate nitrogen-sulfur ligands from Schiff's base disulfides (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1-Alkyl- (or aryl-) amino-2-methylpropane-2-thiols. Bi- and tetradentate nitrogen-sulfur ligands from Schiff's base disulfidesJames L. Corbin and Dale E. WorkCite this: J. Org. Chem. 1976, 41, 3, 489–491Publication Date (Print):February 1, 1976Publication History Published online1 May 2002Published inissue 1 February 1976https://pubs.acs.org/doi/10.1021/jo00865a016https://doi.org/10.1021/jo00865a016research-articleACS PublicationsRequest reuse permissionsArticle Views275Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Digital array planar near-field calibration using element plane wave spectra with iterative search (2018)

Phased array antenna performance (sidelobe level, directivity, null depth, pointing accuracy, etc.) is dependent on calibration quality at each scan angle. Typically phased arrays are calibrated only at broadside scan. In some cases, particularly with smaller, high performance arrays, much time is spent determining calibration coefficients over the scan volume and array weighting, each requiring many pattern measurements. In contrast, digital receive arrays offer the possibility to greatly reduce range time for calibration coefficient extraction. Due to their access to element level I-Q data, superposition can be used to derive the calibration coefficients at all scan angles/weightings (within good measurement practice) with just one near-field scan. In this paper, we derive the theory underpinning the technique, give a calibration algorithm, and show an example array calibrated with the proposed method which drives sidelobe levels (SLLs) to near ideal levels.
A Recent Progress of Spintronics Devices for Integrated Circuit Applications (2018)

Nonvolatile (NV) memory is a key element for future high-performance and low-power microelectronics. Among the proposed NV memories, spintronics-based ones are particularly attractive for applications, owing to their low-voltage and high-speed operation capability in addition to their high-endurance feature. There are three types of spintronics devices with different writing schemes: spin-transfer torque (STT), spin-orbit torque (SOT), and electric field (E-field) effect on magnetic anisotropy. The NV memories using STT have been studied and developed most actively and are about to enter into the market by major semiconductor foundry companies. On the other hand, a development of the NV memories using other writing schemes are now underway. In this review article, first, the recent advancement of the spintronics device using STT and the NV memories using them are reviewed. Next, spintronics devices using the other two writing schemes (SOT and E-field) are briefly reviewed, including issues to be addressed for the NV memories application.
The peroxidase-catalysed oxidation of a chalcone and its possible physiological significance (1972)

1. Crystalline horseradish peroxidase catalysed the oxidation of 2',4,4'-trihydroxychalcone (isoliquiritigenin) in the presence of trace amounts of hydrogen peroxide under aerobic conditions. One atom of oxygen was consumed for each molecule of substrate. 2. The reaction course comprised a lag phase and a linear phase. The optimum pH for the linear phase of the reaction was about 7.5. The length of the lag phase decreased with increasing pH. It is suggested that the chalcone anion is the actual substrate for the reaction. 3. No evidence for the production of reducing free radicals or perhydroxyl radicals during the reaction could be found. 4. 4',7-Dihydroxyflavonol and 4',6-dihydroxyaurone were isolated from the reaction mixture. The immediate products of the reaction may have included 3,4',7-trihydroxyflavanone and 4',6-dihydroxy-2-(alpha-hydroxybenzyl)coumaran-one, which can be readily converted non-enzymically into the flavonol and aurone respectively. 5. A similar reaction was catalysed by cell-free extracts of hypocotyls of Phaseolus vulgaris. 6. The physiological significance of the reaction is discussed in terms of a possible free-radical mechanism. An analogy may exist between flavonoid biosynthesis and lignin formation.
Epidermal Differentiation and Squamous Metaplasia: From Stem Cell to Cell Death (1997)

Epidermal differentiation is a multi-step process defined by a cascade of interrelated changes in the expression of growth-regulatory and differentiation-specific genes (Fig. 1). Irreversible growth arrest is an early event in epidermal differentiation which occurs when cells transit from the basal to the innermost suprabasal layer of the skin and begin to express squamous-specific genes. In culture, interferon gamma, phorbol esters, confluence and growth in suspension are effective signals to induce irreversible growth arrest and differentiation. The induction of differentiation-specific genes occurs either concomitantly with or following growth arrest and is believed to be linked to the molecular events that control irreversible growth arrest. Such a link has been demonstrated in other cell systems undergoing terminal differentiation, such as myogenesis and adipogenesis. Genes encoding proteins involved in the formation of the cross-linked envelope are one set of squamous-specific genes which are induced in the suprabasal layers and include transglutaminase I and III, involucrin, loricrin and cornifins/small proline-rich proteins. Squamous-specific genes exhibit not only different patterns of tissue-specific expression but are also induced at different stages during differentiation, suggesting that transcription of individual genes is regulated by distinct mechanisms. The latter is supported by the identification of different sets of regulatory elements controlling the transcription of these genes. The importance of understanding both the mechanisms that regulate growth arrest and the differentiation program is emphasized by the association found between specific skin diseases and genetic alterations in growth-regulatory genes as well as differentiation markers. In addition, studies into those mechanisms will provide insight into the control of squamous metaplasia and the development of squamous cell carcinomas.