gebhart/patrepeval huggingface dataset:
L-glutamate detection using a poly-L-lysine coated ENFET (2005)

Synaptic transmission in neuronal networks occur on a very short time scale and is highly specific. Fast, sensitive and in situ detection of single neuron L-glutamate release is essential for the investigation of these events under physiological or pathophysiological conditions. Up till now, amperometry with enzyme-modified electrodes has extensively been used to monitor extracellular glutamate release. However, due to in situ signal amplification, ENzyme-modified Field-Effect Transistors (ENFETs) have the advantage of preserving sensitivity and a fast response time when scaled down to micrometer dimensions. We have realized a L-GLutamate OxiDase (GLOD) functionalized FET to be used for glutamate detection in neuronal cultures. Effective and reproducible immobilization of GLOD on the FET active area is achieved by using Poly-L-Lysine (PLL) as a loading matrix. PLL plays a dual role in the assay: on the one hand this molecule serves as a platform for obtaining high enzyme loading and on the other hand it benefits the survival of the neuronal network on the active area of the FET. Both PLL and enzyme immobilization were characterised by quartz crystal microbalance measurements. A much higher enzyme loading has been achieved by this approach compared to immobilization methods without PLL. The enzyme coating has proven to be extremely durable as it keeps its activity for at least 3 weeks as monitored by a colorimetric assay. FET characterisation curves and glutamate response curves of the ENFET are presented.
PWB Z Interconnect Technology - Electrical Performance (2014)

Driven mainly by Moore's law, there is an ever accelerating drive for smaller, lighter, higher function, and lower power electronics. For PWBs this translates into higher wiring and component densities with ever increasing electrical performance requirements. As high speed serial data rates reach 30 Gbps and beyond, design considerations to promote signal integrity become ever more important. These tighter signal integrity constraints put increased emphasis on via stub effects, via to trace crosstalk in BGA escapes, and adequate ground stitch vias around layer transition vias. With the maturation of sintered paste VIAs in PWBs, VIA structures can be assembled to span only the layers to be connected. This paste technology allows vias to be specifically tailored to only connect the required layers. VIA stubs and their adverse SI effects for high speed signaling are completely eliminated. The elimination of via stubs also makes PWB fabrication easier by removing the need to backdrill or counterbore high speed signal vias. This increases yields and reduces costs associated with this complex and tight tolerance process. In addition to manufacturing advantages provided by Z technology, increased wireability is enabled by opening up area above and below the layers being connected, as well as the ability to use smaller diameter VIAs. These smaller vias also help to reduce crosstalk between high speed wiring channels. Z Interconnect technology also reduces BGA escape crosstalk by the ability to route in areas where via stubs have been removed, and also allows for the tailoring of ground stitch vias to only connect the ground planes associated with the specific stripline environments. However, Z VIAs usually require more pads within the padstack than conventional VIAs (cannot strip pads from non-connection layers) and the resistivity of the paste can be as much as 30 times greater than copper. This paper will quantify the high frequency signal characteristics associated with a PWB design using i3 Electronics sintered paste Z-VIAs. Results are presented based on modeling which is correlated with test vehicle measurements. Modeling also addresses manufacturing tolerances. Suggestions are made for optimizing passive channel structures to use this technology in support of high speed serial interconnects. Integration of i3's 2s1p Cores into new compact high speed stripline structures, which can be built without expensive subassemblies and sequential lamination processes, is also presented. These cores implement the positive aspects of Z Interconnect technology to eliminate via stubs and increase wireability in dense areas. Comparisons of Z Interconnect technology are also made to alternate methods of PWB construction to explain the risks and benefits of this technology.
The use of Digitally Reconstructed Radiographs for three-dimensional treatment planning and CT-simulation (1995)

A DRR cannot match the spatial resolution of a radiograph taken with a short exposure and small focal spot, but the ability to change the display mode for the DRR increases the usefulness of these images. Fast reformatting is particularly important when evaluation of field position requires the comparison of each portal image to a series of DRRs.
Efficacy and tolerance of an oral enzyme combination in painful osteoarthritis of the hip. A double-blind, randomised study comparing oral enzymes with non-steroidal anti-inflammatory drugs. (2006)

This trial showed significant non-inferiority from 6 weeks treatment with PE in patients with OA of the hip with regard to the WOMAC dimensions pain, stiffness and physical function, to Lequesne's index, to the investigator and patients assessments of efficacy, and to the responder rates based on pain, physical function, and patient assessment of efficacy. With regard to drug tolerability some tendencies in favour of PE were detected. However, in this study there was no real difference between PE and DC 100 mg/day, implying an equal benefit-risk relation between the substances. PE may well be recommended for the treatment of patients with osteoarthritis of the hip with signs of inflammation as indicated by a high pain level.
Principles, design, implementation (2008)

In private practice, and also affiliated with the U. of Washington School of Dentistry, Cohen nevertheless found time to establish the Seattle Study Club, which meets to address advanced issues in dental treatment planning and has spawned an eponymous quarterly journal. For this reference he invited 17 eminent practitioners to present in detail their approaches to the issues of esthetic adjustments for proportion, display, and length; implant therapy; endodontic predictability; the ideal soft tissue framework; interdisciplinary treatment planning; establishment of a stable occlusal scheme; and the effect of orthognathic surgery on overall treatment, among other topics. The format is oversize (10x13), and the elegantly produced volume contains abundant color photos.
New Era in the Management of Melanoma Brain Metastases (2018)

The remarkable advances in the systemic therapy of metastatic melanoma have now extended the 1-year overall survival rate from 25% to nearing 85%. Systemic treatment in the form of BRAF-targeted therapy and immunotherapy is slowly but surely proving its efficacy in the treatment of metatstatic brain metastases (MBM). Single-agent BRAF inhibitors provide an intracranial response rate of 25% to 40%, whereas the combination of BRAFi/MEKi leads to responses in up to 58%. However, the durability of responses induced by BRAFi/MEKi seems to be even shorter than in extracranial disease. On the other hand, single-agent ipilimumab provides comparable clinical benefit in MBMs as it does in extracranial metastases. Single-agent PD-1 anitbodies induce response rates of approximately 20%, and those responses appear durable. Similarly the combination of CTLA-4+ PD-1 antibodies induces durable responses at an impressive rate of 55% and is safe to administer. Although the local treatment approaches with radiation and surgery remain important and are critically needed in the management of MBM, systemic therapy offers a new dimension that can augment the impact of those therapies and come at a potentially lower cost of neurocognitive impairment. Considerations for combining those modalities are direly needed, in addition to considering novel systemic combinations that target mechanisms specific to MBM. In this report, we will discuss the underlying biology of melanoma brain metastases, the clinical outcomes from recent clinical trials of targeted and immunotherapy, and their impact on clinical practice in the context of existing local therapeutic modalities.
Revolution of the Direct Path (2002)

This lecture is part of the Huston Smith Lecture Series on Religion sponsored by the California Institute of Integral Studies, honoring the life and work of Huston Smith, the internationally known authority on world religions. The lecture series was held at the Unitarian Universalist Church in San Francisco, California, from January 24 through April 11, 2002.
Recently Identified Forms of Epidermolysis Bullosa (2015)

Epidermolysis bullosa (EB) comprises a collection of clinically diverse inherited blistering diseases that affect the skin and, in some subtypes, mucous membranes and other organs. Currently classified into four main subtypes (EB simplex, junctional EB, dystrophic EB, and Kindler syndrome, mainly based on the level of skin cleavage), the spectrum of EB extends to more than 30 clinical subtypes with pathogenic mutations in at least 18 distinct genes. This review focuses on three recent additions to variants of EB: all are autosomal recessive, and result from mutations in either DST-e (coding for epidermal dystonin, also known as the 230 kDa bullous pemphigoid antigen, BP230), EXPH5 (coding for exophilin-5, also known as Slac2-b), or ITGA3 (coding for the integrin alpha-3 subunit). Each of these new forms of EB is reviewed with respect to the initial gene discovery, clinical features, the current mutation database, and skin pathology. Awareness of these recently described forms of EB is helpful in the clinical evaluation of patients with EB and in defining genotype-phenotype correlation for inherited blistering skin diseases.
Prediction of functional impairment and remission in rheumatoid arthritis patients by biochemical variables and genetic polymorphisms (2009)

We have developed two models based on laboratory variables that are associated with relevant outcomes for RA patients at disease onset.
Clinical course and management of accidental adriamycin extravasation (1977)

Adriamycin infiltration into subcutaneous tissues during intravenous administration results in an intense inflammatory response, which may progress to full-thickness skin ioss and irreversible damage to underlying tendons and neurovascular structures. An analysis of 10 patients seen with Adriamycin in filtration indicated that healing of ulcerated lesions is often prolnged and associated with significant morbidity. Seven patients suffered skin ulcerations and three had severe functional impairment due to joint contractures. Adriamycin should not be infused near joints if possible . Surgical excision of ulcers is advocated if healing is prolonged. Proper local therapy, as well as early attention to proper splinting and physical therapy, can help reduce ultimate functional disability.
Compounding artefacts with uncertainty, and an amyloid cascade hypothesis that is ‘too big to fail’ (2011)

With each failure of anti-amyloid-β therapy in clinical trials, new trials are initiated with no hint of slowing down. This may be due, in part, to the fact that the amyloid cascade hypothesis has been so modified over time that it is now impossible to confirm or deny. The hypothesis now states, in effect, that invisible molecules target invisible structures. Still relevant, however, are multiple factors that surely cast some doubt but have either been rationalized or overlooked. Among these are the poor correlation between amyloid-β deposits and disease, the substantial differences between familial and sporadic disease, pathological assessment that indicates the secondary nature of lesions/proteins/cascades, the fact that soluble species are poorly reproducible laboratory phenomena, and the irrelevance of synaptic assessment to pathological interpretation. Although not yet dogma, the premature addition of mild cognitive impairment as the implied in vivo homologue to the soluble toxin-synapse interaction is also problematic. In either case, the amyloid cascade hypothesis continues to dominate the Alzheimer's disease literature and grant applications. The more the neuroscience community perseverates along these lines in the face of accumulating outcome data to the contrary, the more one is left to wonder whether the hypothesis is too big to fail.
A 10 Gbps SerDes for wireless chip-to-chip communication (2015)

This paper presents a 10 Gbps serializer/deserializer (SerDes) with a phase interpolator (PI) based clock and data recovery (CDR) circuit for high-speed and short-range wireless chip-to-chip communication. The SerDes performs 4:1 muxing and 1:4 demuxing functions. The PI-based CDR uses an 8-phase delay-locked loop (DLL) to produce a set of evenly spaced reference clock phases. The phase vernier, then transforms the 8-phases to sampling clocks for the sampler, which performs 2× oversampling to recover the data from the input signal. Implemented in a 65 nm CMOS process, the proposed SerDes achieves a data rate of 10 Gbps and a recovered peak-to-peak clock jitter of 36.25 ps. The 10 Gbps SerDes occupies an active area of 0.095mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and dissipates 88 mW.
Small and Large Faculty-size Adjusted Accounting Program Rankings Based on Research-active Faculty: A Uniform Approach (2015)

Prior studies have ranked accounting programs based on the use of various methodologies, many of which did not control for faculty size. Even in studies that controlled for faculty size, a common issue was the inclusion of faculty and PhD students who were not research active. To resolve these sample issues, this study uses a sample of top-6 accounting journal publications over the 2006-2013 period to demonstrate an innovative, efficient, and uniform approach for calculating faculty-size adjusted accounting program rankings. This approach can be modified to include more accounting journals. Specifically, the study controls for faculty size by including only active researchers at each school: that is, authors who published during this period in one or more of the top-6 accounting journals. Consistent with prior studies, the analyses reveal that controlling for faculty size results in statistically significant changes in program rankings. Other study innovations include separate rankings for large (over 13 faculty members) and small (from 3-13 faculty members) accounting programs. Small school rankings, which have not been the focus of prior research, may provide programs with limited size an important measure of their quality that is potentially useful in recruiting faculty and students.
Analyzing the substrate specificity of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase by co-expressing it with mammalian G protein alpha subunits in Escherichia coli (1991)

A dual plasmid system was used to examine the protein and acyl-CoA specificities of Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (NMT) by co-expressing it in Escherichia coli with each of four homologous alpha subunits of the signal-transducing, heterotrimeric G proteins. Exogenous [3H]myristate was incorporated into rat Gi alpha 1 and rat Go alpha but not into bovine Gs alpha or human Gz alpha. Oxygen for methylene group substitutions in myristate result in analogs with comparable chain length and stereochemistry but marked reductions in hydrophobicity. Metabolic labeling studies with 6-, 11-, or 13-[3H]oxatetradecanoic acid indicated that they were incorporated into rat Gi alpha 1 and Go alpha with an efficiency that could be correlated with their accumulation into E. coli and their interactions with purified NMT in vitro. Octapeptides derived from the NH2-terminal sequences of these four G alpha polypeptides were tested as substrates for purified S. cerevisiae NMT. None were bound by the enzyme. Acidic residues at positions 7 and 8 appear to contribute to this effect; deletion of these two amino acids or addition of the next 9 residues of rat Go alpha produced active substrates. These results imply that productive interactions between NMT and G alpha protein substrates in vivo require structural features that are not fully represented within their NH2-terminal 8 residues.
Diversity interference cancellation using prefiltering and reduced-state MIMO equalization (2004)

We consider a joint detection approach for cancellation of co-channel interference in time-division multiple access (TDMA) mobile communications systems like GSM/EDGE (enhanced data rates for GSM evolution). Concepts from reduced-state equalization of frequency-selective multiple-input multiple-output (MIMO) channels are applied together with prefiltering. A novel efficient prefilter computation algorithm is presented. Simulation results demonstrate the high performance of the proposed receiver for GSM/EDGE applications.
Coordination chemistry of methylmercury(II). Synthesis, hydrogen-1 NMR, and crystallographic studies of cationic complexes of MeHg(II) with ambidentate and polydentate ligands containing pyridyl and N-substituted imidazolyl donors and involving unusual coordination geometries (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCoordination chemistry of methylmercury(II). Synthesis, hydrogen-1 NMR, and crystallographic studies of cationic complexes of MeHg(II) with ambidentate and polydentate ligands containing pyridyl and N-substituted imidazolyl donors and involving unusual coordination geometriesAllan J. Canty, Narongsak Chaichit, Bryan M. Gatehouse, Edwin E. George, and Glen HayhurstCite this: Inorg. Chem. 1981, 20, 8, 2414–2422Publication Date (Print):August 1, 1981Publication History Published online1 May 2002Published inissue 1 August 1981https://pubs.acs.org/doi/10.1021/ic50222a011https://doi.org/10.1021/ic50222a011research-articleACS PublicationsRequest reuse permissionsArticle Views482Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Depletion of Gut-Resident CCR5 <sup>+</sup> Cells for HIV Cure Strategies (2017)

The HIV reservoir forming at the earliest stages of infection is likely composed of CCR5<sup>+</sup> cells, because these cells are the targets of transmissible virus. Restriction of the CCR5<sup>+</sup> reservoir, particularly in the gut, may be needed for subsequent cure attempts. Strategies for killing or depleting CCR5<sup>+</sup> cells have been described, but none have been tested in vivo in nonhuman primates, and the extent of achievable depletion from tissues is not known. In this study we investigate the efficacy of two novel cytotoxic treatments for targeting and eliminating CCR5<sup>+</sup> cells in young rhesus macaques. The first, an immunotoxin consisting of the endogenous CCR5 ligand RANTES fused with Pseudomonas exotoxin (RANTES-PE38), killed CCR5<sup>+</sup> lamina propria lymphocytes (LPLs) ex vivo, but had no detectable effect on CCR5<sup>+</sup> LPLs in vivo. The second, a primatized bispecific antibody for CCR5 and CD3, depleted all CCR5<sup>+</sup> cells from blood and the vast majority of such cells from the colonic mucosa (up to 96% of CD4<sup>+</sup>CCR5<sup>+</sup>). Absence of CCR5-expressing cells from blood endured for at least 1 week, while CCR5<sup>+</sup> cells in colon were substantially replenished over the same time span. These data open an avenue to investigation of combined early ART treatment and CCR5<sup>+</sup> reservoir depletion for cure of HIV-infected infants.
A Real-Time Quantitative PCR Detection Method for Pork, Chicken, Beef, Mutton, and Horseflesh in Foods (2007)

A rapid real-time quantitative PCR method to detect trace amounts of pork, chicken, beef, mutton, and horseflesh in foods was developed. The primers and TaqMan MGB (minor groove binder) probes were designed on the gene encoding cytochrome b for the specific detection of each species. The limit of quantification of this method was found to be 100 fg/microl of each mitochondrial DNA in 10 ng/microl of the wheat mitochondrial DNA matrix. The calculated R(2) values of the standard curves for the five species ranged between 0.994 and 0.999. This method would be particularly useful in the detection of hidden meat mince in processed foods, which would verify food labeling and gain consumers' trust.
Upper bound performance of adaptive modulation ina slow Rayleighfading channel (1996)

The upper bound bit error rate (BER) performance of a time division duplex (TDD) system, where the number of modulation levels is adapted on a very short term basis, depending upon the channels conditions, is derived and solved by numerical integration. The performance shows very close correspondence with simulated results.
Frequent concomitant epigenetic silencing of the stress‐responsive tumor suppressor gene <i>CADM1</i>, and its interacting partner <i>DAL‐1</i> in nasal NK/T‐cell lymphoma (2008)

Nasal NK/T-cell lymphoma (NL) is a rare but clinically important entity of lymphoma. Its preferential incidence in Orientals but not Caucasians suggests possible genetic predisposition. 11q deletion is common in NL, indicating certain tumor suppressor genes (TSGs) at this locus involved in its pathogenesis. We investigated the expression and methylation of an 11q23.2 TSG, CADM1 (or TSLC1), and its partner DAL-1 (or EPB41L3) in NL. Methylation and silencing of CADM1 were detected in 2 NL and 4 of 8 (50%) of non-Hodgkin lymphoma (NHL) cell lines, but not in normal NK cells and normal PBMC. Absence of CADM1 protein was also detected in NL cell lines. 5-aza-2'-deoxycytidine (Aza) demethylation or genetic knockout of both DNMT1 and 3B genes restored CADM1 and DAL-1 expression. CADM1 methylation was further detected in 36 of 45 (80%) of NL tumors. Concomitantly, DAL-1 was epigenetically inactivated in NL cell lines and virtually all the tumors with methylated CADM1. A significant correlation between the methylation of both genes was found (p < 0.0001). Homozygous deletion of CADM1 was detected in only 3 of 18 (17%) of tumors. The stress-response of CADM1 was abolished when its promoter becomes methylated. Our results demonstrate a frequent, predominant epigenetic silencing of CADM1 and DAL-1 in NL, which likely play a synergic role in NL pathogenesis.
Direct Interconversion of BINOL and H8-BINOL-Based Chiral Brønsted Acids Using Single-Step Red/Ox Manipulations (2015)

A direct single-step hydrogenation of BINOL-based chiral phosphoric acids, N-triflyl phosphoramides, and disulfonimides to the corresponding H8-BINOL Brønsted acids in excellent yields and chemoselectivities is described. In addition, the conditions for the single-step oxidation of H8-BINOL-based Brønsted acids into the corresponding BINOL-based acids have been identified and employed to accomplish these interconversions in 41-81% yield.