gebhart/patrepeval huggingface dataset:
Interfacial Nanobubbles Are Leaky: Permeability of the Gas/Water Interface (2014)

Currently there is no widespread agreement on an explanation for the stability of surface nanobubbles. One means by which several explanations can be differentiated is through the predictions they make about the degree of permeability of the gas-solution interface. Here we test the hypothesis that the gas-solution interface of surface nanobubbles is permeable by experimental measurements of the exchange of carbon dioxide. We present measurements by attenuated total reflection Fourier transform infrared (ATR-FTIR) and atomic force microscopy (AFM), demonstrating that the gas inside surface nanobubbles is not sealed inside the bubbles, but rather exchanges with the dissolved gas in the liquid phase. Such gas transfer is measurable by using the infrared active gas CO2. We find that bubbles formed in air-saturated water that is then perfused with CO2-saturated water give rise to distinctive gaseous CO2 signals in ATR-FTIR measurements. Also the CO2 gas inside nanobubbles quickly dissolves into the surrounding air-saturated water. AFM images before and after fluid exchange show that CO2 bubbles shrink upon exposure to air-equilibrated liquid but remain stable for hours. Also air bubbles in contact with CO2-saturated water increase in size and Ostwald ripening occurs more rapidly due to the relatively high gas solubility of CO2 in water.
Introduction to Data (2021)

Data is quite a broad term that can include any informative piece of information. For statistical analyses, data are most often organized into variables, which refer to anything that can take on more than one value. Outside of SPSS, the social and behavioral sciences commonly utilize the Stevens taxonomy of nominal, ordinal, interval, and ratio scales of measurement to govern the assignment of numbers to variable values. SPSS allows a great deal of flexibility in what students consider to be data. In addition to string and numeric variables, there are several others that can be stored within SPSS. Data are typically entered and stored within SPSS as a matrix of information. SPSS is primarily concerned with variables and the entities that provide values for those variables.
Transparently interposing user code at the system interface (2003)

The author reports on the development of an object-oriented toolkit which substantially increases the ease of interposing user code between clients and instances of the system interface by allowing such code to be written in terms of the high-level objects provided by this interface, rather than in terms of the intercepted system calls themselves. This toolkit helps enable new interposition agents to be written which otherwise would not have been attempted. It is used to construct several agents including protected environments for running untrusted binaries, modified file system namespaces, logical devices implemented entirely in user space, transparent network data compression and/or encryption agents, and system call tracing tools. Examples of other agents which could be built include transactional software environments and emulators for other operating system environments.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Subunit-based mucosal vaccine delivery systems for pulmonary delivery - Are they feasible? (2019)

Pulmonary infections are the most common cause of death globally. However, the development of mucosal vaccines that provide protective immunity against respiratory pathogens are limited. In contrast to needle-based vaccines, efficient vaccines that are delivered via noninvasive mucosal routes (such as via the lungs and nasal passage) produce both antigen-specific local mucosal IgA and systemic IgG protective antibodies. One major challenge in the development of pulmonary vaccines using subunit antigens however, is the production of optimal immune responses. Subunit vaccines therefore rely upon use of adjuvants to potentiate immune responses. While the lack of suitable mucosal adjuvants has hindered progress in the development of efficient pulmonary vaccines, particle-based systems can provide an alternative approach for the safe and efficient delivery of subunit vaccines. In particular, the rational engineering of particulate vaccines with optimal physicochemical characteristics can produce long-term protective immunity. These protect antigens against enzymatic degradation, target antigen presenting cells and initiate optimal humoral and cellular immunity. This review will discuss our current understanding of pulmonary immunology and developments in fabricating particle characteristics that may evoke potent and durable pulmonary immunity.
Green Fluorescent Protein as a Selectable Marker of Retrovirally Transduced Hematopoietic Progenitors (1999)

Recombinant retroviruses are most commonly used in hematopoietic stem cell gene therapy trials, but gene transfer efficiency is still inadequate with the present vectors. One approach for overcoming this problem is to develop methods of selecting and enriching the successfully transduced cells. We investigated the feasibility of using the green fluorescent protein (GFP) gene as a selectable marker of hematopoietic cells. When M1 murine leukemia cells were electroporated with GFP expression vectors, a red-shifted mutant (S65T) GFP showed several-fold greater fluorescence than the wild-type GFP and generated readily detectable green light under control of SRalpha or CAG promoter. We then inserted an SRalpha-S65T GFP cassette into the MSCV retrovirus vector and established virus producer cells. Infection of primary murine bone marrow cells resulted in a distinct population with green fluorescence, which was separated by fluorescence-activated cell sorting. The fractionated bright cells gave rise to fluorescent spleen colonies in lethally irradiated mice, while the fluorescence-negative cells yielded only dark colonies. These results indicated that GFP is a faithful marker in gene transfer into hematopoietic progenitor/stem cells, facilitating selection of the transduced cells and tracking of their progeny in vivo.
Integrating probabilistic extraction models and data mining to discover relations and patterns in text (2006)

In order for relation extraction systems to obtain human-level performance, they must be able to incorporate relational patterns inherent in the data (for example, that one's sister is likely one's mother's daughter, or that children are likely to attend the same college as their parents). Hand-coding such knowledge can be time-consuming and inadequate. Additionally, there may exist many interesting, unknown relational patterns that both improve extraction performance and provide insight into text. We describe a probabilistic extraction model that provides mutual benefits to both "top-down" relational pattern discovery and "bottom-up" relation extraction.
Magic Gold Nanotubes (2005)

In recent ultra-high-vacuum transmission-electron-microscopy experiments evidence is found for the formation of suspended gold single-wall nanotubes (SWNTs) composed of five helical strands. Similar to carbon nanotubes, the (n,m) notation defines the structure of the gold SWNTs. Experimentally, only the (5,3) tube has been observed to form among several other possible alternatives. Using first-principles calculations we demonstrate that gold atoms can form both freestanding and tip-suspended, chiral, single-wall nanotubes. Although freestanding, infinite (5,5) tube is found to be energetically the most favorable, the experimentally observed (5,3) tube, suspended between two tips, corresponds to a local minimum in the variation of string-tension with the radius of the structure, which explains the experimental finding. Similarly, we predict the (4,3) tube as a favorable structure yet to be observed experimentally. Analysis of band structure, charge density, and quantum ballistic conductance suggests that the current on these nanowires is less chiral than expected, and there is no direct correlation between the numbers of conduction channels and helical strands. © TÜBİTAK.
Combinatorial materials research applied to the development of new surface coatings III. Utilisation of a high-throughput multiwell plate screening method to rapidly assess bacterial biofilm retention on antifouling surfaces (2007)

The authors recently reported on the development of a novel multiwell plate screening method for the high-throughput assessment of bacterial biofilm retention on surfaces. Two series of biocide containing coatings were prepared to assess the ability of the developed assay to adequately discern differences in antifouling performance: i) a commercially available poly(methyl methacrylate) (PMMA) and silicone elastomer (DC) physically blended with an organic antifouling biocide Sea-Nine 211 (SN211) (4,5-dichloro-2-n-octyl-3(2H)-isothiazolone), and ii) a silanol-terminated polydimethylsiloxane (PDMS-OH) reacted with an alkoxy silane-modified polyethylenimine containing bound ammonium salt groups (PEI-AmCl). Three marine bacteria were utilised to evaluate the SN211 blended coatings (Pseudoalteromonas atlantica ATCC 19262, Cobetia marina ATCC 25374, Halomonas pacifica ATCC 27122) and the marine bacterium Cytophaga lytica was utilised to evaluate the PEI-AmCl/PDMS-OH coatings. The SN211 blended coatings showed a general trend of decreasing biofilm retention as the concentration of SN211 increased in both PMMA and DC. HPLC analysis revealed that reduction in biofilm retention was positively correlated with the amount of SN211 released into the growth medium over the length of the bacterial incubation. When compared to PMMA, DC consistently showed an equal or greater percent reduction in biofilm retention as the level of SN211 loading increased, although at lower loading concentrations. Evaluations of the PEI-AmCl/PDMS-OH coatings with C. lytica showed that all PEI-AmCl loading concentrations significantly reduced biofilm retention (p<0.0001) by a surface contact phenomenon. The high-throughput bacterial biofilm growth and retention assay has been shown to be useful as an effective primary screening tool for the rapid assessment of antifouling materials.
In-situ laser spectroscopy of CO, Ch4, and H2O in a particle laden laboratory-scale fluidized bed combustor (2002)

The pyrolysis, devolatilization and char combustion of bituminous coal and biomass (beechwood, firwood) were investigated in a laboratory-scale fluidized bed combustor by tunable diode laser spectroscopy. Individual fuel particles were suspended in the freeboard of the unit. The bed temperature was 800 ?C, the oxygen partial pressure 0 to 20 kPa (0-10 vol.%). Two Fabry Perot type tunable near infrared diode lasers were deployed for quantitative in-situ species concentration measurements. CH4 and CO were measured simultaneously during devolatilization and char combustion in-situ 10 mm above the surface of the fuel particles as well as H2O using laser spectroscopy. Sand particles were passing the probing laser beam path. Besides the resonant absorption of the laser light by CO, CH4 and H2O severe and strongly transient non-resonant attenuation by partial blocking of the beam and beam steering effects occurred. By wavelength tuning the two laser sources, species concentrations could be determined. The measured absorbances had to be corrected for the real temperature measured at the position of the probing laser beam. In addition, CO, CO2 and O2 were determined ex-situ by con ventional methods. A spatial profile inside the FBC of major species (CH4, CO, CO2, O, H, OH) was calculated using a chemical kinetics program for a single fuel particle in a plug flow reactor geometry. The results were compared to the experimental findings. Good agreement was found. Tunable diode laser spectroscopy was found to be an apt method of determining quantitative species concentrations of multiple gases in a high temperature multi phase environment.
A Beginner's Guide to Partial Least Squares Analysis (2004)

Since the introduction of covariance-based structural equation modeling (SEM) by Joreskog in 1973, this technique has been received with considerable interest among empirical researchers. However, the predominance of LISREL, certainly the most well-known tool to perform this kind of analysis, has led to the fact that not all researchers are aware of alternative techniques for SEM, such as partial least squares (PLS) analysis. Therefore, the objective of this article is to provide an easily comprehensible introduction to this technique, which is particularly suited to situations in which constructs are measured by a very large number of indicators and where maximum likelihood covariance-based SEM tools reach their limit. Because this article is intended as a general introduction, it avoids mathematical details as far as possible and instead focuses on a presentation of PLS, which can be understood without an in-depth knowledge of SEM.
Cerebral adaptations to chronic anemia in a model of erythropoietin-deficient mice exposed to hypoxia (2008)

Anemia and hypoxia in rats result in an increase in factors potentially involved in cerebral angiogenesis. Therefore, the aim of this study was to assess the effect of chronic anemia and/or chronic hypoxia on cerebral cellular responses and angiogenesis in wild-type and anemic transgenic mice. These studies were done in erythropoietin-deficient mice (Epo-TAg(h)) in normoxia and following acute (one day) and chronic (14 days, barometric pressure = 420 mmHg) hypoxia. In normoxia, Epo-TAg(h) mice showed an increase in transcript and protein levels of hypoxia-inducible factor 1alpha (HIF-1alpha), vascular endothelial growth factor (VEGF), erythropoietin receptors (EpoR), phospho-STAT-5/STAT-5 ratio, and neuronal neuronal nitric oxide synthase (nNOS) along with a higher cerebral capillary density. In wild-type (WT) mice, acute hypoxia increased all of the studied factors, while in chronic hypoxia, HIF-1alpha, EpoR, phospho-STAT-5/STAT-5 ratio, nNOS, and inducible NOS remained elevated, with an increase in capillary density. Surprisingly, in Epo-TAg(h) mice, chronic hypoxia did not further increase any factor except the nitric oxide metabolites, while HIF-1alpha, EpoR, and phospho-STAT-5/STAT-5 ratio were reduced. Normoxic Epo-TAg(h) mice developed cerebral angiogenesis through the HIF-1alpha/VEGF pathway. In acute hypoxia, WT mice up-regulated all of the studied factors, including cerebral NO. Polycythemia and angiogenesis occurred with acclimatization to chronic hypoxia only in WT mice. In Epo-TAg(h), the decrease in HIF-1alpha, VEGF proteins, and phospho-STAT-5 ratio in chronic hypoxia suggest that neuroprotective and angiogenesis pathways are altered.
The Second Cytoplasmic Loop of Metabotropic Glutamate Receptor Functions at the Third Loop Position of Rhodopsin (2001)

G protein-coupled receptors identified so far are classified into at least three major families based on their amino acid sequences. For the family of receptors homologous to rhodopsin (family 1), the G protein activation mechanism has been investigated in detail, but much less for the receptors of other families. To functionally compare the G protein activation mechanism between rhodopsin and metabotropic glutamate receptor (mGluR), which belong to distinct families, we prepared a set of bovine rhodopsin mutants whose second or third cytoplasmic loop was replaced with either the second or third loop of Gi/Go- or Gq-coupled mGluR (mGluR6 or mGluR1). Among these mutants, the mutants in which the second or third loop was replaced with the corresponding loop of mGluR exhibited no G protein activation ability. In contrast, the mutant whose third loop was replaced with the second loop of Gi/Go-coupled mGluR6 efficiently activated Gi but not Gt: this activation profile is almost identical with those of the mutant rhodopsins whose third loop was replaced with those of the Gi/Go-coupled receptors in family 1 [Yamashita et al. (2000) J. Biol. Chem. 275, 34272-34279]. The mutant whose third loop was replaced with the second loop of Gq-coupled mGluR1 partially retained the Gi coupling ability of rhodopsin, which is in contrast to the fact that all the rhodopsin mutants having the third loops of Gq-coupled receptors in family 1 exhibit no detectable Gi activation. These results strongly suggest that the molecular architectures of rhodopsin and mGluR are different, although the G protein activation mechanism involving the cytoplasmic loops is common.
An analysis of the evidence‐practice continuum: is surgery for obstructive sleep apnoea contraindicated? (2007)

This case study demonstrates substantial procedural variability and limited effectiveness. This raises questions as to the quality of care, the treatment-derived health outcomes of this population and of efficient resource allocation. This issue requires greater policy attention.
Studies Towards the Synthesis of Peptide-Oligonucleotide Conjugates (1999)

Abstract We describe a peptide fragment, solid-phase coupling strategy for synthesis of peptide-oligonucleotide conjugates. Model conjugates contained a hydrophobic tetrapeptide, a hydrophobic influenza virus fusion nonapeptide, or a basic octapeptide of the HIV-1 Tat protein coupled to either dT12 or a 16-mer anti-Tat oligodeoxyribonucleotide. Conjugation yields were improved by removal of internucleotide 2-cyanoethyl groups prior to peptide coupling and by use of a C12 spacer between peptide and oligonucleotide.
Teaching, Learning, and Using Spectroscopy with Commercial, Off-the-Shelf Technology (2010)

The astonishing propagation of sophisticated electronics across the globe has attracted the attention of many for developing disease diagnoses, water purity measurements, and security applications using cellular telephones and the complementary metal oxide semiconductor (CMOS) cameras with which many are equipped. This Focal Point article builds on the theme of using technology already in the hands of students/consumers to teach spectrophotometry and, by extension, to suggest how one might inexpensively develop an instrumental analysis curriculum at extraordinarily low cost. A grating spectrophotometer using a white light-emitting diode (LED) light source, plastic sample cuvette, holographic transmission grating, and any camera that produces JPG files as output is described. Optical characteristics are explained and a sample working curve provided. The raw data for that curve are available as Supplementary Material on-line. The behavior of the "Cell Phone Spectrometer" is inadequate for routine analysis but outstanding for teaching the workings of optical instrumentation components and systems.
The Preparation, Orientation and Electronic Structure of Poly(p-phenylenevinylene) (PPV) Monolayers on Silicon Surfaces (1997)

Article The Preparation, Orientation and Electronic Structure of Poly(p-phenylenevinylene) (PPV) Monolayers on Silicon Surfaces was published on January 1, 1997 in the journal Zeitschrift für Physikalische Chemie (volume 202, issue 1-2).
Yeast ribosomal DNA genes are located on chromosome XII. (1979)

Two lines of experimental evidence indicate that the repeating ribosomal DNA (rDNA) genes of the yeast Saccharomyces cerevisiae are located on chromosome XII. First, the rDNA genes are linked mitotically to genes that have been previously mapped to chromosome XII. Second, yeast strains that have two copies of the chromosome containing the rDNA genes in every strain examined also have two copies of chromosome XII; this is not true for the other yeast chromosomes. These data also establish that in mitosis most of the rDNA genes in yeast are not extrachromosomal.
NARROW CHANNEL EFFECT ON n- AND p-CHANNEL DEVICES FABRICATED WITH THE SILO AND BOX ISOLATION TECHNIQUES (1988)

We have studied the narrow channel effect (NCE) on active devices fabricated with the SILO and BOX isolation techniques. The importance of the boron encroachment in the active region is shown, as well as the advantage of the BOX technique which uses a deposited oxide instead of a thermal oxide in the SILO process. With a low field implantation dose used to reduce the NCE, a double threshold effect in the subthreshold characteristic is inevitable.
Support for workspace awareness in educational groupware (1995)

Article Free Access Share on Support for workspace awareness in educational groupware Authors: Carl Gutwin View Profile , Gwen Stark View Profile , Saul Greenberg View Profile Authors Info & Claims CSCL '95: The first international conference on Computer support for collaborative learningOctober 1995 Pages 147–156https://doi.org/10.3115/222020.222126Online:01 October 1995Publication History 50citation274DownloadsMetricsTotal Citations50Total Downloads274Last 12 Months11Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Confocal Microscopy Theory and Applications (2003)

This chapter will attempt to provide an overview of the theory and details of some basic applications of the technique of confocal microscopy. Since this is intended to be a general introduction to the possibilities available, it is not appropriate to include detailed descriptions of the specialized techniques used in physiological studies leading to the imaging and measurement of Ca2+-containing samples. Instead, the reader is referred to Chapter 5 in this volume that deals specifically with these imaging techniques. In this chapter our aim is to define the properties of the instrumentation and to give a few examples of basic preparation techniques for defining structure and localizing target molecules in cells. As one example, however, we will refer to our simple imaging technique for studying the three-dimensional (3D) microscopic distribution of calcium during skeletogenesis in the mammalian fetus.