gebhart/patrepeval huggingface dataset:
Variants of a Leishmania surface antigen derived from a multigenic family. (1991)

The promastigote surface antigen-2 (PSA-2) complex comprises a group of immunogenic surface antigens linked to the surface of the Leishmania major promastigote with glycosylphosphatidylinositol anchors. The L. major genome contains at least 14 PSA-2 genes on a 950-kilobase chromosome and comprising approximately 20% of the length of this chromosome. The sequence of three independent, but incomplete, PSA-2 cDNAs and one genomic fragment encoding a complete PSA-2 coding sequence were compared. PSA-2 genes encode polypeptides exhibiting 22-25aa tandem repeat elements, threonine-rich segments which vary between genes, a conserved COOH-terminal cysteine-rich region, and a conserved GPI anchor signal sequence. PSA-2 genes appear to be transcribed in a complex manner with multiple RNAs. The complex genomic organization of PSA-2 genes is present in other members of the genus suggesting that PSA-2 function is important for the biology of Leishmania.
Characterization of phospholipase A2 (PLA2) activity in gerbil brain: enhanced activities of cytosolic, mitochondrial, and microsomal forms after ischemia and reperfusion (1991)

Brain phospholipase A2 (PLA2) activity has not been well characterized. Given the importance of this enzymatic activity for a variety of cellular functions in the brain, we characterized the subcellular distribution of PLA2 activity in gerbil brain and evaluated how PLA2 activity was altered by ischemia and reperfusion. Cytosolic, mitochondrial, and microsomal fractions were prepared by differential centrifugation of forebrain homogenates. PLA2 activities of each fraction were assayed by measuring release of arachidonic acid (AA) from exogenous 14C-AA-phosphatidylcholine (PC), -phosphatidylethanolamine (PE), and -phosphatidylinositol (PI). Two forms of PLA2 were present in the cytosolic fraction: a high-molecular-weight form, active against PC and PE, and a smaller form with an Mr of approximately 14 kDa, active against PE. In the mitochondrial and microsomal fractions, a single form (Mr approximately 14 kDa) was dominant, active against both PC and PE. The role of PLA2 activation in ischemic brain injury remains controversial. PLA2 enzymatic activity was characterized in gerbil brain after 10 min of common carotid occlusion, followed by 10 min of reperfusion. Ischemic/reperfused brains had significantly higher PLA2 specific activities in each subcellular fraction. Ischemia and reperfusion did not change the gel-filtration elution patterns of PLA2 activity of the various forms of the enzyme. Cytosolic, mitochondrial, and microsomal activities were optimal at a pH of approximately 8.5. Cytosolic PLA2 activity was enhanced when Ca2+ concentration [( Ca2+]) was increased over the physiological range (10(-7) to 10(-6) M). Mitochondrial and microsomal PLA2 activities were also [Ca2+] dependent.(ABSTRACT TRUNCATED AT 250 WORDS)
Modulation of drug resistance by artificial transcription factors (2008)

The efficiency of chemotherapeutic treatments in cancer patients is often impaired by the acquisition of drug resistance. Cancer cells develop drug resistance through dysregulation of one or more genes or cellular pathways. To isolate efficient regulators of drug resistance in tumor cells, we have adopted a genome-wide scanning approach based on the screening of large libraries of artificial transcription factors (ATFs) made of three and six randomly assembled zinc finger domains. Zinc finger libraries were linked to a VP64 activation domain and delivered into a paclitaxel-sensitive tumor cell line. Following drug treatment, several ATFs were isolated that promoted drug resistance. One of these ATFs, 3ZF-1-VP, promoted paclitaxel resistance in cell lines having mutated or inactivated p53, such as MDA-MB-435 and Kaposi's sarcoma cell lines. 3ZF-1-VP also induced strong resistance to etoposide, vincristine, and cisplatinum. Linkage of a repression domain to the selected ATF resulted in enhanced sensitivity to multiple drugs, particularly vincristine, cisplatinum, and 5-fluorouracil. Small interfering RNA-mediated inhibition of p53 revealed that 3ZF-1-VP activated both p53-dependent and p53-independent mechanisms to promote survival, whereas other ATF required intact p53. Real-time expression analysis and DNA microarrays showed that several ATFs up-regulated targets of p53, such as the cyclin-dependent kinase inhibitor p21(WAF1/CIP1), and genes participating in the p14(ARF)-MDM2-p53 tumor suppressor pathway, such as hDMP1. Thus, ATF can be used to map genes and pathways involved in drug resistance phenotypes and have potential as novel therapeutic agents to inhibit drug resistance.
NMR Study of the Solution Structure of Curcumin (2007)

Curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] is derived from the rhizomes of Curcuma longa. Although early studies concluded that curcumin exists predominantly as a keto-enol tautomer, 1b, in several recent articles the solution structure of curcumin has been represented as a beta-diketone tautomer, 1a. We have investigated the structure of curcumin in solvents ranging in polarity from CDCl3 to mixtures of DMSO-d6 in water, and in buffered aqueous DMSO-d6 solutions with pH values varying from 3 to 9. The solution structure of curcumin was determined on the basis of NMR techniques, including DEPT, HMQC, HMBC, and COSY. The results of the NMR studies show definitely that curcumin exists in solution as keto-enol tautomers, 1b.
Ultralong, Well‐Aligned Single‐Walled Carbon Nanotube Architectureson Surfaces (2003)

A novel fast‐heating chemical vapor deposition (CVD) method to grow ultralong, well‐aligned single‐walled carbon nanotubes (SWNTs) is developed. The control of lengths and growth directions provided by this CVD technique is used to fabricate ordered, large‐scale nanotube structures. The Figure shows an ultralong SWNT.
Clinical Outcome of Autologous Chondrocyte Implantation at 5 Years in US Subjects (2005)

Therapeutic study, Level II-1 (prospective cohort study). See the Guidelines for Authors for a complete description of levels of evidence.
Application of laser-induced breakdown spectroscopy to <italic>in situ</italic> analysis of liquid samples (2000)

A realization of laser-induced breakdown spectroscopy for real-time, in situ and remote analysis of trace amounts in liquid samples is described, which is potentially applicable to the analysis of pollutants in water in harsh or difficult-to-reach environments. Most of the measurements were conducted using a fiber assembly that is capable of both delivering the laser light and collecting the light emitted from the micro plasma, up to about 30 m from the target area. Alternatively, a telescopic arrangement for line-of-sight measurements was employed, with a range of 3 to 5 m. For internal standardization and the generation of concentration calibration curves, reference lines of selected elements were used. In the majority of cases calibration against the matrix element hydrogen was employed using the H?, H?, and H? lines, but also spiking with selected reference species was utilized. In order to provide high reliability and repeatability in the analyses, we also measured plasma parameters such as electron density, plasma temperature, and line- shape functions, and determined their influence on the measurement results. Numerous elements, including a range of toxic heavy metals, have been measured over a wide range of concentrations (Al, Cr, Cu, Pb, Tc, U, and others). Limits of detection usually were in the range of a few parts per million; for several elements even lower concentrations could be measured.
Statistical Block Protection Coding for DPCM-AQF Speech (1980)

The statistical block protection coding scheme [2] for protecting DPCM encoded speech signals through noisy channels has been extended to accommodate DPCM-AQF encoded speech, where AQF stands for adaptive quantization with forward (explicit) transmission of step size. Signal-to-noise ratio (SNR) gains, typically 12 dB, have been achieved over a dynamic range <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">> 20</tex> dB for a bit error rate (BER) of 1.4 percent and the SNR improvement is found to increase with BER. Perceptual improvements in decoded speech have a good correspondence with the gains in SNR. The penalty for this substantial enhancement in the performance of the DPCM-AQF system is an increase in transmission bit rate of 3.5 percent and encoding delay of 64 ms.
New series representation for the trivariate non-central chi-squared distribution (2009)

This paper derives a new infinite series representation for the trivariate non-central chi-squared distribution when the underlying correlated Gaussian variables have a tridiagonal form of an inverse covariance matrix. The joint probability density function is derived using Miller's approach and Dougall's identity. Moreover, the trivariate cumulative distribution function (cdf) and characteristic function (chf) are also derived. Finally, the bivariate non-central chi-squared distribution and some known forms are shown to be special cases of the more general distribution. However, the derivation of non-central chi-squared distribution for an arbitrary covariance matrix seems intractable via Miller's approach. Two applications of the newly derived results are provided for performance analysis of multiple input multiple output (MIMO) systems with transmit antenna selection over a correlated Rician fading environment. Some numerical results are also presented to verify the accuracy of the analytical expressions.
Calcium oscillations in human T and natural killer cells depend upon membrane potential and calcium influx (1993)

With the use of single-cell digital imaging of the fluorescent Ca2+ indicator dye fura-2 we investigated Ca2+ signaling in human T lymphocytes and NK cells during activation by a variety of stimuli. A low percentage of resting T cells or T cell blasts displayed oscillations in cytosolic Ca2+ when stimulated with the mitogenic lectin PHA or by the addition of OKT3 mAb followed by a secondary cross-linking antibody. Lymphokine-activated T killer cells were more responsive than resting cells. A comparison of PHA, cross-linked anti-CD3, and a heteroconjugate mAb showed that at least 20% of the cells from these T cell preparations oscillated. Addition of PHA or cross-linked anti-CD16 caused NK cells to oscillate. In contrast, thapsigargin, a microsomal ATPase blocker, resulted in a relatively uniform, slowly rising and sustained Ca2+ response in all cell types studied. The maintenance of both thapsigargin- and receptor-induced responses required Ca2+ influx driven by a negative membrane potential. Because Ca2+ oscillations occurred in response to stimuli which mimic the normal activation of lymphocytes, and inasmuch as the percentage of oscillating cells increases with state of activation, these oscillations may play an important role in mitogenic activation.
Blood Group Alloantibodies: An Assessment of Some Laboratory Practices (1977)

Over a 20-year period, the number of blood donors and transfusion recipients previously immunized by blood group antigens was determined in a large blood center. During that period, the proportion of patients found to have anti-D declined, while those with other antibodies in the Rh system, as well as in the Kell, Duffy, and Kidd systems, increased sharply. As expected, the rate of sensitization was much lower among blood donors than among recipients. In both groups, sensitized females greatly outnumbered sensitized males. Some of the findings summarized here raise questions about current practices in transfusion laboratories and emphasize the responsibilities of blood bank professionals to donors as well as to patients.
A measure of term representativeness based on the number of co-occurring salient words (2002)

We propose a novel measure of the representativeness (i.e., indicativeness or topic specificity) of a term in a given corpus. The measure embodies the idea that the distribution of words co-occurring with a representative term should be biased according to the word distribution in the whole corpus. The bias of the word distribution in the co-occurring words is defined as the number of distinct words whose occurrences are saliently biased in the co-occurring words. The saliency of a word is defined by a threshold probability that can be automatically defined using the whole corpus. Comparative evaluation clarified that the measure is clearly superior to conventional measures in finding topic-specific words in the newspaper archives of different sizes.
Time-multiplexed three-dimensional displays based on directional backlights with fast-switching liquid-crystal displays (2006)

An autostereoscopic display using a directional backlight with a fast-switching liquid-crystal (LC) display was designed and fabricated to obtain a better perception of 3D images by enhanced resolution and brightness. A grooved light guide in combination with an asymmetric focusing foil was utilized to redirect the emitting cones of light to the left and right eyes, respectively. By designing the groove structures of the focusing foil with rotation from -1.5 degrees to 1.5 degrees in the gradient and having the pitch ratio of the grooved light guide to the focusing foil of less than 3, the boundary angle then shifts from normal viewing and the moiré phenomenon can be suppressed. Cross talk of less than 6% and a LC response time of faster than 7.1 ms further improve the stereoscopic image perception. Additionally, 2D-3D compatibility is provided.
Moisture Uptake Impact on Damage Layer of Porous Low-k Film in 80nm-Pitched Cu Interconnects (2011)

Impacts of the moisture uptake into the damage layer of porous SiOCH low-k film on line capacitance and leakage current were investigated in the 80nm-pitch triple layer interconnect structure. The damage layer thickness was controlled from zero to 8nm by the RIE, wet process and Cu surface treatment. The damage layer thickness is defined by TEM-EELS analysis. The line capacitance and leakage current were increased by moisture uptake. The dielectric constant of damage layer including moisture was estimated 7.6 by the capacitance simulation. The moisture control of the damage layer in porous SiOCH low-k film is very important to reduce the line capacitance and improve the line leakage current as well as damage layer elimination.
ChemInform Abstract: Novel AMPA Receptor Antagonists: Synthesis and Structure‐Activity Relationships of 1‐Hydroxy‐7‐(1H‐imidazol‐1‐yl)‐6‐nitro‐2,3(1H,4H) quinoxalinedione and Related Compounds. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Chemical composition, antimicrobial activity and antioxidant capacity of some medicinal and aromatic plant extracts (2013)

In this study, the chemical composition, antimicrobial activity and antioxidant capacity of essential oils obtained from oregano (Origanum onites L.), sage (Salvia triloba L.), mint (Mentha piperita L.), and laurel (Laurus nobilus L.) were determined. The essential oil components were identified by gas chromatography (GC) analysis. The antimicrobial activity of the oils was determined against some pathogenic and lactic acid bacteria using a disc diffusion method. The total antioxidant capacity was evaluatedspectrophotometrically as α-tocopherol equivalent. The GC analysis showed that the major constituents of the oils were monoterpene hydrocarbons and phenolic monoterpenes, but the concentration of these compounds varied greatly among the oils examined. The main components of the essential oil were carvacrol, menthol, 1-8 cineole, thujon, camphor and menthone. The results of the antimicrobial assay showed that majority of essential oils showed varied levels of antimicrobial activity against the tested indicator strains. Also, all essential oils exhibited antioxidant effect on oils used in the experiment. The results of this study confirmed the possibility of using some essential oils in food as preservation systems for growth of foodborne bacteria, improving antioxidant properties and extend the shelf-life of processed foods.   Key words: Essential oil, chemical composition, antimicrobial activity, antioxidant capacity, medicinal and aromatic plants
Physicochemical and Sensory Properties of Corn Tortillas Made from Nixtamalized Corn Flour Fortified with Spent Soymilk Residue (okara) (2002)

ABSTRACT: The chemical study of okara composition and a sensory evaluation study were conducted to determine the effect of okara supplementation on certain physical, chemical and sensory properties of corn tortilla. Dried okara was added at the level of 5,10,15,20, and 25% to nixtamalized corn flour. Due to flavor, enriched tortillas at levels higher than 10% of okara were not accepted. At 10% of okara supplement, lysine and tryptophan content in tortilla were increased from 56 and 70% of FAO profile to 93 and 92% respectively.
The <i>Caenorhabditis elegans</i> death protein Ced‐4 contains a motif with similarity to the mammalian ‘death effector domain’ (1997)

In the nematode Caenorhabditis elegans apoptosis is tightly regulated by a hierarchical set of genes. Two of these, ced-3 and ced-9, possess mammalian homologues encoding executional ICE proteases and inhibitory Bcl-2-related proteins, respectively. The function of a third key player, ced-4, is however completely unknown and no mammalian counterparts have been identified. Here we report that Ced-4 protein contains a structural region with similarity to the mammalian death effector domain which has previously been demonstrated to act as an important protein interaction motif in the signaling pathway of the mammalian surface receptor Fas (APO-1, CD95). Based on this finding and previously described genetic experiments, we propose that Ced-4, similar to the mammalian proteins FADD and FLICE, may possess a function as an adaptor protein in invertebrate apoptotic pathways.
A research on Video text tracking and recognition (2013)

Nowadays, video has gradually become the mainstream of dissemination media for its rich information capacity and intelligibility, and texts in videos often carry significant semantic information, thus making great contribution to video content understanding and construction of content-based video retrieval system. Text-based video analyses usually consist of text detection, localization, tracking, segmentation and recognition. There has been a large amount of research done on video text detection and tracking, but most solutions focus on text content processing in static frames, few making full use of redundancy between video frames. In this paper, a unified framework for text detection, localization and tracking in video frames is proposed. We select edge and corner distribution of text blocks as text features, localizing and tracking are performed. By making good use of redundancy between frames, location relations and motion characteristics are determined, thus effectively reduce false-alarm and raise correct rate in localizing. Tracking schemes are proposed for static and rolling texts respectively. Through multi-frame integration, text quality is promoted, so is correct rate of OCR. Experiments demonstrate the reduction of false-alarm and the increase of correct rate of localization and recognition.
Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis. (1991)

Random cell migration and actin organization in seven human primary, recurrent cutaneous, and metastatic melanoma cell lines were studied by time-lapse video recording and image analysis. The migration of over 800 randomly selected cells from the cell lines were recorded using an inverted microscope with an attached incubator housing. The fraction of cells with random migration rates greater than 10 microns/hour was 8% in an established primary melanoma cell line, 2% and 34% in two recurrent cutaneous melanoma cell lines, and 5%, 30%, 31%, and 60% in four metastatic cell lines. The three metastatic cell lines with significantly higher mean migration rates (P less than 0.001) were derived from lymph node metastases, whereas the fourth metastatic cell line was derived from a visceral metastasis. The cellular morphology and presence of cell nests in the original tissue correlated with in vitro cell morphology and the formation of colonies. The ability of cells to organize actin into stress fibers directly correlated with significantly higher random migration rates and lack of colony formation. Characterization of random migration rates and actin organization of human melanoma cells that are isolated from different stages of tumor progression may lend insight into metastasis.
Genomics of glycopeptidolipid biosynthesis in Mycobacterium abscessus and M. chelonae (2007)

Although these three species produce identical GPL molecules, the organization of GPL genes differ between them, thus constituting species-specific signatures. An hypothesis is that the compact organization of the GPL locus in M. smegmatis represents the ancestral form and that evolution has scattered various pieces throughout the genome in M. abscessus and M. chelonae.
Preparation and properties of some polyglycerol esters of short and medium chain length fatty acids (1971)

Abstract Mono‐ and diesters of the short and medium chain acids were prepared with polyglycerols and compared with their corresponding mono‐ and diglycerides. The physical and chemical properties of such partial esters of polyglycerols, particularly with respect to their oil, water and solvent solubility are given. Polyglycerol esters become more hydrophilic as the molecular weight of the polyol increases. Polyglycerol esters become less hydrophilic as the length of the aliphatic chain of the acid used in esterification is increased. Polyglycerol esters of such short and medium chain fatty acids show promise in applications where surface activity and emulsification, solubilization of oil and water systems is desired.
Comparative arylation reactions with pentaphenylbismuth and with triphenylbismuth carbonate (1980)

Pentaphenylbismuth is a useful reagent for the specific ortho-phenylation of phenols under very mild conditions, a comparison of this reagent with triphenylbismuth carbonate being drawn; arylation reactions of the carbonate have been extended to include nitro-compounds and amides.
Effect of production conditions on the stability of a human bifidobacterial species<i>Bifidobacterium longum</i>in yogurt (2009)

This investigation indicates that stable probiotic yogurt using human bifidobacteria can be produced by choosing optimal production conditions.
Simple printed monopole slot antenna for WWAN mobile handset (2011)

A simple printed monopole slot antenna for penta-band WWAN operation (824~960/ 1710~2170 MHz) in the mobile handset is presented. The monopole slot has a length of 50 mm only and is embedded close to the bottom edge of the system ground plane of the handset with a small distance of 11 mm. By adding a small-size C-shaped strip connected orthogonal to the bottom edge of the system ground plane, good excitation of the monopole slot antenna at its fundamental (0.25-wavelength) and higher-order resonant modes can be obtained. The excited resonant modes form two wide operating bands to respectively cover the GSM850/900 operation (824~960 MHz) and GSM1800/1900/UMTS operation (1710~2170 MHz). Operating principle and radiation characteristics including the SAR (specific absorption rate) results of the proposed monopole slot antenna are described. Measured results of the fabricated antenna are also presented.