gebhart/patrepeval huggingface dataset:
Gene Transfer of the Co‐Stimulatory Molecules B7‐1 and B7‐2 Enhances the Immunogenicity of Human Renal Cell Carcinoma to a Different Extent (1999)

Stimulation of a specific antitumour immune response with recruitment and induction of T-cell effector functions represents an attractive concept in human cancer therapy. Different cytokines and the B7 co-stimulatory molecules are both able to provide proliferation and activation signals for T cells. In the present study, we first demonstrated the absence of both B7-1 and B7-2 expression in human renal cell carcinoma (RCC) cell lines. The lack of B7 expression was associated with a low or absent proliferative response of allogeneic and autologous T cells upon stimulation with tumour cells. In order to investigate the role of B7-1 and B7-2, the human RCC cell line, MZ1257RC, which expresses normal levels of adhesion molecules and major histocompatibility complex (MHC) class I surface antigens, was transfected with B7-1 and B7-2 expression vectors, respectively. The B7-1- and B7-2-transduced MZ1257RC cells were potent stimulators of allogeneic and autologous T-cell proliferation. B7-2 transfectants were approximately two- to threefold more effective in the induction of primary T-cell activation than B7-1-transduced cells. Interleukin (IL)-12 synergized with the B7/CD28 interaction to enhance allogeneic T-cell proliferation, independently of the B7 molecule transduced. In contrast, IL-2 only co-operatively increased T-cell activation in the presence of B7-2. Our results suggest the following: first, that co-stimulatory molecules are required for efficient T-cell responses directed against RCC; second, that B7-2 appears to be a more potent stimulator of tumour immunity as compared to B7-1; and third, that B7 molecules selectively co-operate with different T-cell stimulatory cytokines. The different activity of B7-1 and B7-2 molecules on the immunogenicity of RCC will have implications for the development and optimization of RCC-specific cancer vaccines.
The indoleamine 2,3-dioxygenase pathway controls complement-dependent enhancement of chemo-radiation therapy against murine glioblastoma (2014)

Together these findings identify a novel mechanistic link between IDO and complement, and implicate complement as a major downstream effector mechanism for the beneficial effect of IDO-blockade after chemo-radiation therapy. We speculate that this represents a fundamental pathway by which the tumor regulates intratumoral vascular activation and protects itself from immune-mediated tumor destruction.
Fast parametric elastic image registration (2003)

We present an algorithm for fast elastic multidimensional intensity-based image registration with a parametric model of the deformation. It is fully automatic in its default mode of operation. In the case of hard real-world problems, it is capable of accepting expert hints in the form of soft landmark constraints. Much fewer landmarks are needed and the results are far superior compared to pure landmark registration. Particular attention has been paid to the factors influencing the speed of this algorithm. The B-spline deformation model is shown to be computationally more efficient than other alternatives. The algorithm has been successfully used for several two-dimensional (2-D) and three-dimensional (3-D) registration tasks in the medical domain, involving MRI, SPECT, CT, and ultrasound image modalities. We also present experiments in a controlled environment, permitting an exact evaluation of the registration accuracy. Test deformations are generated automatically using a random hierarchical fractional wavelet-based generator.
Time-Triggered Fieldbus Networks State of the Art and Future Applications (2008)

The time-triggered paradigm encompasses a set of concepts and principles that support the design of dependable real-time systems. By using the properties of physical time and the mechanism of clock synchronization, coordinated interaction between distributed nodes can be facilitated. This paper briefly explains the time-triggered approach, defines a taxonomy for real-time requirements and discusses seven protocols that provide time-triggered features. Finally, two application examples are given that show the potential of the time-triggered approach.
Antioxidant Properties of Extracts from Medicinal Plants Popularly Used in Taiwan (2005)

We have examined antioxidant activities of twenty-six medicinal herbal extracts that have been popularly used as folk medicines in Taiwan. The results of scavenging DPPH radical activity show that, among the 26 tested medicinal plants, Ludwigia octovalvis, Vitis thunbergii, Rubus parvifolius, Lindernia anagallis, and Zanthoxylum nitidum exhibited strong activities and their IC50 values for DPPH radicals were 4.6, 24, 27, 36, 50 g/mL, respectively. As for the su- peroxide anion scavenging activity (IC50, g/mL), the top five most significant activities were observed in plant extracts of Ludwigia octovalvis (26  g/mL), Vitis thunbergii (58  g/mL), Prunella vulgaris (113  g/mL), Saurauia oldhamii (124  g/mL), and Rubus parvifolius (151  g/mL). The IC50 values for DPPH and superoxide anion of catechin (positive control) were 2.5 and 7.2  g/mL, respectively. It was also observed in the present study that, at 1 mg/mL, Ludwigia octovalvis and Bombax malabaricum exhibited significant protection onψx174 supe r- coiled DNA against strand cleavage induced by UV irradiated H2O2 with a superior or compati- ble effect to that of catechin.
Protein-tyrosine Phosphatases Are Involved in Interferon Resistance Associated with Insulin Resistance in HepG2 Cells and Obese Mice (2012)

Insulin resistance is a risk factor for non-response to interferon/ribavirin therapy in patients with chronic hepatitis C. The aim of this study was to determine the role played by protein-tyrosine phosphatases (PTPs) in the absence of interferon-α (IFNα) response associated with insulin resistance. We induced insulin resistance by silencing IRS-2 or by treating HepG2 cells with tumor necrosis factor-α (TNFα) and analyzed insulin response by evaluating Akt phosphorylation and IFNα response by measuring Stat-1 tyrosine phosphorylation and 2',5'-oligoadenylate synthase and myxovirus resistance gene expression. The response to IFNα was also measured in insulin-resistant obese mice (high fat diet and ob/ob mice) untreated and treated with metformin. Silencing IRS-2 mRNA induces insulin resistance and inhibits IFNα response. Likewise, TNFα suppresses insulin and IFNα response. Treatment of cells with pervanadate and knocking down PTP-1B restores insulin and IFNα response. Both silencing IRS-2 and TNFα treatment increase PTP and PTP-1B activity. Metformin inhibits PTP and improves IFNα response in insulin-resistant cells. Insulin-resistant ob/ob mice have increased PTP-1B gene expression and activity in the liver and do not respond to IFNα administration. Treatment with metformin improves this response. In HepG2 cells, insulin resistance provokes IFNα resistance, which is associated with an increased PTP-1B activity in the liver. Inhibition of PTP-1B activity with pervanadate and metformin or knocking down PTP-1B reestablishes IFNα response. Likewise, metformin decreases PTP-1B activity and improves response to IFNα in insulin-resistant obese mice. The use of PTP-1B inhibitors may improve the response to IFNα/ribavirin therapy.
High-Yield Synthesis of Monomeric LMWP(CPP)-siRNA Covalent Conjugate for Effective Cytosolic Delivery of siRNA (2017)

Because of the unparalleled efficiency and universal utility in treating a variety of disease types, siRNA agents have evolved as the future drug-of-choice. Yet, the inability of the polyanionic siRNA macromolecules to cross the cell membrane remains as the bottleneck of possible clinical applications. With the cell penetrating peptides (CPP) being discovered lately, the most effective tactic to achieve the highest intracellular siRNA delivery deems to be by covalently conjugating the drug to a CPP; for instance, the arginine-rich Tat or low molecular weight protamine (LMWP) peptides. However, construction of such a chemical conjugate has been referred by scientists in this field as the "Holy Grail" challenge due to self-assembly of the cationic CPP and anionic siRNA into insoluble aggregates that are deprived of the biological functions of both compounds. Based on the dynamic motion of PEG, we present herein a concise coupling strategy that is capable of permitting a high-yield synthesis of the cell-permeable, cytosol-dissociable LMWP-siRNA covalent conjugates. Cell culture assessment demonstrates that this chemical conjugate yields by far the most effective intracellular siRNA delivery and its corresponded gene-silencing activities. This work may offer a breakthrough advance towards realizing the clinical potential of all siRNA therapeutics and, presumably, most anionic macromolecular drugs such as anti-sense oligonucleotides, gene compounds, DNA vectors and proteins where conjugation with the CPP encounters with problems of aggregation and precipitation. To this end, the impact of this coupling technique is significant, far-reaching and wide-spread.
Extraction and Solubilization of Proteins for Proteomic Studies (2005)

For any proteomic study involving various control and experimental specimens, several factors need to be in place. A critical one is the extraction and solubilization of all components, regardless of whether a chromatographic (1,2) or two-dimensional (2-D) gel electrophoretic fractionation (3–6) is performed prior to analysis of proteins of interest by mass spectrometry of protein digests. All proteins must not only be extracted, but they must also be completely soluble, free from interacting partners (such as protein-RNA/DNA and protein-protein interactions, metabolites, and so on), and, in the case of 2-D gel electrophoresis, they must remain soluble as they approach their isoelectric points. The solubilization process should extract all classes of proteins reproducibly, such that statistically significant quantitative data can be obtained and correlated with experimental perturbations and the resulting biological responses.
Recovery of Motile Sperm Using the Osmolality-Gradient Technique (1991)

A simple, effective method for collecting motile sperm from ejaculates is developed. One hundred semen samples were obtained from both fertile and infertile men. Following initial semen dilution (1: 1) to 4 mL in a hypoosmotic medium (210 mOsm/kg), the mixture was layered into the specially designed tube filled with 1 mL recovery medium in the bottom and 1 mL isolation medium in the second layer to form three discrete layers. During the next hour the highly motile sperm out-distanced the other seminal contents to reach the bottom of the tube. Compared with the parent ejaculates, the recovered specimens exhibited an increased percentage of motile sperm with significantly enhanced sperm velocity and linearity (p less than .001). This method isolated motile sperm with higher velocity than those obtained using the swim-up technique.
Artificial neural network and empirical mode decomposition based imbalance fault diagnosis of wind turbine using TurbSim, FAST and Simulink (2016)

In this study, an artificial neural network (ANN) and empirical mode decomposition (EMD) based condition monitoring approach of a wind turbine using Simulink, FAST (fatigue, aerodynamics, structures and turbulence) and TurbSim is presented. The complete dynamics of a permanent magnet synchronous generator (PMSG) based wind turbine [i.e. wind turbine generator (WTG)] model is simulated in an amalgamated domain of Simulink, FAST and TurbSim under six distinct conditions, i.e. aerodynamic asymmetry, rotor‐furl imbalance, tail‐furl imbalance, blade imbalance, nacelle‐yaw imbalance and normal operating scenarios. The simulation results in time domain of the PMSG output stator current are decomposed into the intrinsic mode functions using EMD method then RapidMiner‐based principal component analysis method is used to select most relevant input variables. An ANN model is then proposed to differentiate the normal operating scenarios from five fault conditions. The analysed results proclaim the effectiveness of the proposed approach to identify the different imbalance faults in WTG. The presented work renders initial results that are helpful for online condition monitoring and health assessment of WTG.
Enhancement of Reflectionless Filters Using Stop-Band Sub-Networks (2014)

This paper expands on the previously described reflectionless filters - that is, filters having, in principle, identically-zero reflection coefficient at all frequencies - by introducing a wide variety of new reflectionless structures that were not part of the original publication. In addition to extending the lumped-element derivation to include transmission line filters, this is achieved by the introduction of a novel method wherein the left- and right-hand side stop-band terminations are coupled to each other using a two-port sub-network. Specific examples of the sub-network are given which increase the stop-band attenuation per filter cell and steepen the cutoff response without disrupting the reflectionless property or increasing the insertion-loss in the pass-band. It is noteworthy that a common feature of all the structures derived by these methods is that most, if not all, of the reactive elements are of equal normalized value. This greatly simplifies the tuning requirements, and has facilitated their implementation as monolithic microwave integrated circuits (MMICs). A number of examples of MMIC reflectionless filters constructed in this way are presented and their results compared to the theory.
Fabrication of PMMA CE microchips by infrared‐assisted polymerization (2008)

In this report, a method based on the infrared-assisted polymerization of methyl methacrylate has been developed for the rapid fabrication of PMMA CE microchips. Methyl methacrylate containing AIBN was allowed to prepolymerize in a water bath to form a fast-curing molding solution that was subsequently sandwiched between a silicon template and a piece of 1 mm-thick PMMA plate. The images of microchannels on the silicon template were precisely replicated into the synthesized PMMA substrates during the infrared-assisted polymerization of the molding solution. The polymerization could be completed within 50 min at 50 degrees C. The obtained channel plate was subsequently bonded to a piece of PMMA cover sheet to form a microchip with the aid of heat and pressure. The new fabrication approach obviates the need for special equipment and significantly simplifies the process of fabricating PMMA microchips. The attractive performance of the obtained PMMA microchips has been demonstrated in connection with contactless conductivity detection for the separation and detection of ionic species.
Introduction (1972)

This number of Soviet Psychology is devoted entirely to the work of Bluma V. Zeigarnik as summarized in her monograph Personality and the pathology of activity.
The ubiquitin proteolytic system and pathogenesis of human diseases: a novel platform for mechanism-based drug targeting (2003)

Until the early 1980s, protein degradation was a neglected research area, and scientists were mostly busy deciphering the genetic code and its translation to the proteome. Destruction of cellular proteins was thought to be a scavenger, non-specific and dead-end process. Although it was known that proteins do turn over, the large extent and high specificity of the process, whereby distinct proteins have half-lives that range from a few minutes to several days, was not appreciated. The discovery of the lysosome by Christian de Duve did not change this view significantly, as it was clear that this organelle is involved mostly in the degradation of extracellular proteins, and their proteases cannot be substrate-specific. The discovery of the complex cascade of the ubiquitin pathway revolutionized the field. It is clear now that degradation of cellular proteins via the ubiquitin system is a highly complex, temporally controlled and tightly regulated process that plays major roles in a variety of basic pathways and processes during cell life and death, and in health and disease. The system is involved in targeting many cellular proteins, among them cell cycle regulators, growth- and differentiation-controlling factors, transcriptional activators, cell-surface receptors and ion channels, endoplasmic reticulum proteins, antigenic proteins destined for presentation on class I MHC molecules, and abnormal/misfolded proteins. Consequently, it is involved in regulating many basic cellular processes, such as cell cycle and division, growth and differentiation, signal transduction and transcription, modulation of the secretory and endocytic pathways, the immune and inflammatory responses, and quality control. With the multitude of substrates targeted and the numerous processes involved, it is not surprising that aberrations in the pathway have been implicated in the pathogenesis of many diseases, with certain malignancies and neurodegenerative disorders being ranked among them.
Palatability, Digestibility and Voluntary Intake of Processed Oil Palm Fronds in Cattle (2001)

Preference tests and digestion trials of 4 different types of fronds of oil palm (Elaeis guineensis Jacq.), i.e. oven-dried form, pellets, silage and sodium hydroxide (NaOH)-treated silage, were carried out to analyze the influence of processing methods on the palatability, digestibility and voluntary intake in cattle. These processed oil palm fronds (OPFs) for digestion trials at maintenance level or under ad libitum feeding were mixed with a basal ration, mainly composed of palm kernel cake and fed to Kedah-Kelantan cross cattle. The digestibility of OPFs per se was calculated from the digestibility of the mixed ration and that of the basal ration. The results were as follows; the digestibility of OPF pellets was considerably lower than that of the other types of processed OPFs. However, OPF pellets had a high palatability and the dry matter intake (DMI) of the mixed ration, which includes OPF pellets, was higher than that of the other types of processed OPFs. These findings suggest that OPF pellets could be utilized as a silage additive to increase the dry matter content of crops with a high moisture level. The digestibility and DMI of ensiling OPFs were equivalent to those of oven-dried OPFs and the palatability of ensiling OPFs was high. Ensiling is the most suitable method to preserve OPFs at a low cost and can be recommended to farmers. NaOH-treated silage had a higher digestible DMI than the other types of processed OPFs, although it had a low palatability. It was confirmed that alkali treatment can effectively improve the nutritive value of OPFs. Therefore, introducing alkali treatment to the production of processed OPFs should be promoted if a safe and cost-effective method is to be developed.
Surface Modification of Poly(L-lactic acid) Nanofiber with Oligo(D-lactic acid) Bioactive-Peptide Conjugates for Peripheral Nerve Regeneration (2011)

In some traumatic nerve injuries, autologous nerve grafting is the first choice for bridging the gap between the severed nerve ends. However, this therapeutic strategy has some disadvantages, including permanent loss of donor function and requirement of multiple surgeries. An attractive alternative to this therapeutic technique is the use of artificial nerve conduit. Poly (L-lactic acid) (PLLA) is widely used as a substrate for artificial nerve conduit because it is readily biodegradable, but it is not inherently biologically active. In this study, we developed a PLLA nanofibrous nerve conduit, modified with a conjugate of oligo (D-lactic acid) (ODLA) and the neurite outgrowth, thereby promoting peptide AG73 (RKRLQVQLSIRT) to improve nerve regeneration. PLA/ODLA-AG73 nanofibrous conduit was fabricated by electrospinning and then transplanted at the 10 mm gap of rat sciatic nerve. After six months, electrophysiological evaluation revealed that it achieved better functional reinnervation than silicone tube (used as a reference) or unmodified PLLA nanofibrous conduit.
Characterization of dendritic cell differentiation pathways from cord blood CD34+CD7+CD45RA+hematopoietic progenitor cells (2000)

To better characterize human dendritic cells (DCs) that originate from lymphoid progenitors, the authors examined the DC differentiation pathways from a novel CD7+CD45RA+ progenitor population found among cord blood CD34+ cells. Unlike CD7−CD45RA+ and CD7+CD45RA− progenitors, this population displayed high natural killer (NK) cell differentiation capacity when cultured with stem cell factor (SCF), interleukin (IL)-2, IL-7, and IL-15, attesting to its lymphoid potential. In cultures with SCF, Flt3 ligand (FL), granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor (TNF)-α (standard condition), CD7+CD45RA+ progenitors expanded less (37- vs 155-fold) but yielded 2-fold higher CD1a+ DC percentages than CD7−CD45RA+ or CD7+CD45RA− progenitors. As reported for CD34+CD1a− thymocytes, cloning experiments demonstrated that CD7+CD45RA+ cells comprised bipotent NK/DC progenitors. DCs differentiated from CD7−CD45RA+ and CD7+CD45RA+ progenitors differed as to E-cadherin CD123, CD116, and CD127 expression, but none of these was really discriminant. Only CD7+CD45RA+ or thymic progenitors differentiated into Lag+S100+Langerhans cells in the absence of exogenous transforming growth factor (TGF)-β1. Analysis of the DC differentiation pathways showed that CD7+CD45RA+ progenitors generated CD1a+CD14− precursors that were macrophage-colony stimulating factor (M-CSF) resistant and CD1a−CD14+ precursors that readily differentiated into DCs under the standard condition. Accordingly, CD7+CD45RA+ progenitor-derived mature DCs produced 2- to 4-fold more IL-6, IL-12, and TNF-α on CD40 ligation and elicited 3- to 6-fold higher allogeneic T-lymphocyte reactivity than CD7−CD45RA+ progenitor-derived DCs. Altogether, these findings provide evidence that the DCs that differentiate from cord blood CD34+CD7+CD45RA+ progenitors represent an original population for their developmental pathways and function.
Oxidative stress, free radicals and bone remodeling (2008)

Reactive oxygen species (ROS) are widely considered to be a causal factor in aging and in a number of pathological conditions, such as atherosclerosis, carcinogenesis and infarction. Their role in bone metabolism is dual, considering their effects under physiological or pathological conditions. Under physiological conditions, the production of ROS by osteoclasts helps accelerate destruction of calcified tissue, thus assisting in bone remodeling. In pathological conditions, when a bone fractures, e.g., radical generation is remarkably high. However, though the increases in osteoclastic activity and ROS production are linked in many skeletal pathologies, it remains to be clarified whether increased ROS production overwhelms antioxidant defenses, leaving the individual open to hyperoxidant stress.