gebhart/patrepeval huggingface dataset:
Celastrol Induced DNA Damage, Cell Cycle Arrest, and Apoptosis in Human Rheumatoid Fibroblast-Like Synovial Cells (2013)

Celastrol is one of the principal active ingredients of Tripterygium wilfordii Hook.f., a toxic Chinese medical herb traditionally prescribed for controlling pain and inhibiting inflammation in various chronic inflammatory diseases, including rheumatoid arthritis (RA). Resistance to apoptosis of fibroblast-like synoviocytes is considered a major characteristic of RA. In this study, we test celastrol's cytotoxic effect and potential mechanisms in human rheumatoid synovial fibroblasts (RA-FLS). In the cytotoxic assay, we found that celastrol dose-dependently decreased RA-FLS viability and increased LDH release. The apoptotic nuclear morphology was observed after celastrol treatment as determined by DAPI fluorescence staining. Flow cytometry analysis with PI and Annexin V revealed that celastrol induced RA-FLS cell cycle arrest in the G2/M phase and apoptosis. Furthermore, celastrol dramatically increased expression of Bax/Bcl-2, proteolytic cleavage of Caspase-3, -9, PARP, and decreased expression of FasR. In addition, celastrol treatment resulted in DNA damage. Collectively, we concluded that celastrol inhibits RA-FLS proliferation by inducing DNA damage, cell cycle arrest, and apoptosis in vitro, which might provide data for its application in RA treatment.
Deterministic initialization of metric state estimation filters for loosely-coupled monocular vision-inertial systems (2011)

In this work, we present a novel, deterministic closed-form solution for computing the scale factor and the gravity direction of a moving, loosely-coupled, and monocular vision-inertial system. The methodology is based on analysing delta-velocities. On one hand, they are obtained from a differentiation of the up-to-scale camera pose computation by a visual odometry or visual SLAM algorithm. On the other hand, they can also be retrieved from the gravity-affected short-term integration of acceleration signals. We derive a method for separating the gravity contribution and recovering the metric scale factor of the vision algorithm. The method thus also recovers the offset in roll and pitch angles of the vision reference frame with respect to the direction of the gravity vector. It uses only a single inertial integration period, and no absolute orientation information is required. For optimal sensor-fusion and metric scale-estimation filters in the loosely-coupled case, it has been shown that the convergence of the fusion of an up-to-scale pose information with inertial measurements largely depends on the availability of a good initial value for the scale factor. We show how this problem can be tackled by applying the method presented in this paper. Finally, we present results in simulation and on real data, demonstrating the suitability of the method in real scenarios.
Biological Activities of Polyphenols from Grapes (2010)

The dietary consumption of grape and its products is associated with a lower incidence of degenerative diseases such as cardiovascular disease and certain types of cancers. Most recent interest has focused on the bioactive phenolic compounds in grape. Anthocyanins, flavanols, flavonols and resveratrol are the most important grape polyphenols because they possess many biological activities, such as antioxidant, cardioprotective, anticancer, anti-inflammation, antiaging and antimicrobial properties. This review summarizes current knowledge on the bioactivities of grape phenolics. The extraction, isolation and identification methods of polyphenols from grape as well as their bioavailability and potential toxicity also are included.
Enzymatic amplification in a bioinspired, autonomous signal cascade (2006)

A two-step reaction cascade is applied to the sequence-specific detection of DNA by enzymatic amplification of the molecular into an optical signal.
Immunobiology of the complement system : an introduction for research and clinical medicine (1986)

Immunobiology of the Complement System: An Introduction for Research and Clinical Medicine provides an introduction to the complement system. The intention was to create a primer that would provide the basic knowledge of complement required for either research or clinical medicine in diseases involving the complement system
<title>Production-ready silicon microvalve</title> (1999)

Micromachining is envisioned as an enabling technology for the miniaturization of chemical analysis systems. It has been pursued for gas phase analysis (GPA) in HP's Chemical Analysis Group and at HP Labs. A thermally actuated, silicon micromachined gas control valve has been jointly developed at HPL and Little Falls Analytical Division (LFAD) for use in a gas chromatograph. This microvalve has 1/10 the weight and 1/5 the package volume of competing solenoid valves. This low cost, high reliability, proportional gas control valve reduces the cost, weight and size of a crucial component for a gas chromatograph system. This paper discusses valve structure and performance, and the resolution of issues over the course of the development.
294. Aliphatic nitro-compounds. Part XV. Preparation of heterocyclic bases by reduction of 3-nitroalkyl cyanides (1947)

G. D. Buckley and T. J. Elliott, J. Chem. Soc., 1947, 1508 DOI: 10.1039/JR9470001508
Image statistics do not explain the perception of gloss and lightness (2009)

A fundamental problem in image analysis is to understand the nature of the computations and mechanisms that provide information about the material properties of surfaces. Information about a surface's 3D shape, optics, illumination field, and atmospheric conditions are conflated in the image, which must somehow be disentangled to derive the properties of surfaces. It was recently suggested that the visual system exploits some simple image statistics-histogram or sub-band skew-to infer the lightness and gloss of surfaces (I. Motoyoshi, S. Nishida, L. Sharan, & E. H. Adelson, 2007). Here, we show that the correlations Motoyoshi et al. (2007) observed between skew, lightness, and gloss only arose because of the limited space of surface geometries, reflectance properties, and illumination fields they evaluated. We argue that the lightness effects they reported were a statistical artifact of equating the means of images with skewed histograms, and that the perception of gloss requires an analysis of the consistency between the estimate of a surface's 3D shape and the positions and orientations of highlights on a surface. We argue that the derivation of surface and material properties requires a photo-geometric analysis, and that purely photometric statistics such as skew fail to capture any diagnostic information about surfaces because they are devoid of the structural information needed to distinguish different types of surface attributes.
Subthreshold Schottky-barrier thin-film transistors with ultralow power and high intrinsic gain (2016)

The quest for low power becomes highly compelling in newly emerging application areas related to wearable devices in the Internet of Things. Here, we report on a Schottky-barrier indium-gallium-zinc-oxide thin-film transistor operating in the deep subthreshold regime (i.e., near the OFF state) at low supply voltages (<1 volt) and ultralow power (<1 nanowatt). By using a Schottky-barrier at the source and drain contacts, the current-voltage characteristics of the transistor were virtually channel-length independent with an infinite output resistance. It exhibited high intrinsic gain (>400) that was both bias and geometry independent. The transistor reported here is useful for sensor interface circuits in wearable devices where high current sensitivity and ultralow power are vital for battery-less operation.
Phenotypic and functional analysis of murine CD3+,CD4-,CD8- TCR-gamma delta-expressing peripheral T cells. (1989)

Murine CD3+,CD4-,CD8- peripheral T cells, which express various forms of the TCR-gamma delta on their cell surface, have been characterized in terms of their cell-surface phenotype, proliferative and lytic potential, and lymphokine-producing capabilities. Three-color flow cytofluorometric analysis demonstrated that freshly isolated CD3+,CD4-, CD8- TCR-gamma delta lymph node cells were predominantly Thy-1+,CD5dull,IL-2R-,HSA-,B220-, and approximately 70% Ly-6C+ and 70% Pgp-1+. After CD3+,CD4-,CD8-splenocytes were expanded for 7 days in vitro with anti-CD3-epsilon mAb (145-2C11) and IL-2, the majority of the TCR-gamma delta cells expressed B220 and IL-2R, and 10 to 20% were CD8+. In comparison to CD8+ TCR-alpha beta T cells, the population of CD8+ TCR-gamma delta-bearing T cells exhibited reduced levels of CD8, and about 70% of the CD8+ TCR-gamma delta cells did not express Lyt-3 on the cell surface. Functional studies demonstrated that splenic TCR-gamma delta cells proliferated when stimulated with mAb directed against CD3-epsilon, Thy-1, and Ly-6C, but not when incubated with an anti-TCR V beta 8 mAb, consistent with the lack of TCR-alpha beta expression. In addition, activated CD3+,CD4-,CD8- peripheral murine TCR-gamma delta cells were capable of lysing syngeneic FcR-bearing targets in the presence of anti-CD3-epsilon mAb and the NK-sensitive cell line, YAC-1, in the absence of anti-CD3-epsilon mAb. Finally, activated CD3+, CD4-,CD8-,TCR-gamma delta+ splenocytes were also capable of producing IL-2, IL-3, IFN-gamma, and TNF when stimulated in vitro with anti-CD3-epsilon mAb.
Mining California vital statistics data (2002)

Vital statistics data offer a fertile ground for data mining. The authors discuss the results of a data mining project on the causes of death aspect of the vital statistics data in the state of California. A data mining tool called Cubist is used to build predictive models out of two million cases over a nine-year period. The objective of our study is to discover knowledge that can be used to gain insight into various aspects of mortality in California, to predict health issues related to the causes of death, to offer an aid to decision- or policy-making processes, and to provide useful information services to the customers. The results obtained in our study contain valuable new information.
Three-Component Intramolecular Two-Alkyne Annulations of Fischer Carbene Complexes: New Strategies for Steroid Synthesis (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThree-Component Intramolecular Two-Alkyne Annulations of Fischer Carbene Complexes: New Strategies for Steroid SynthesisJianming Bao, William D. Wulff, Vera Dragisich, Steve Wenglowsky, and Richard G. BallCite this: J. Am. Chem. Soc. 1994, 116, 17, 7616–7630Publication Date (Print):August 1, 1994Publication History Published online1 May 2002Published inissue 1 August 1994https://pubs.acs.org/doi/10.1021/ja00096a019https://doi.org/10.1021/ja00096a019research-articleACS PublicationsRequest reuse permissionsArticle Views761Altmetric-Citations64LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Comparison of HIV- and EIAV-Based Vectors on Their Efficiency in Transducing Murine and Human Hematopoietic Repopulating Cells (2005)

The use of lentiviral vectors for gene transfer into hematopoietic stem cells has raised considerable interest as these vectors can permanently integrate their genome into quiescent cells. Vectors based on alternative lentiviruses would theoretically be safer than HIV-1-based vectors and could also be used in HIV-positive patients, minimizing the risk of generating replication-competent virus. Here we report the use of third-generation equine infectious anemia virus (EIAV)- and HIV-1-based vectors with minimal viral sequences and absence of accessory proteins. We have compared their efficiency in transducing mouse and human hematopoietic stem cells both in vitro and in vivo to that of a previously documented second-generation HIV-1 vector. The third-generation EIAV- and HIV-based vectors gave comparable levels of transduction and transgene expression in both mouse and human NOD/SCID repopulating cells but were less efficient than the second-generation HIV-1 vector in human HSCs. For the EIAV vector this is possibly a reflection of the lower protein expression levels achieved in human cells, as vector copy number analysis revealed that this vector exhibited a trend to integrate equally efficiently compared to the third-generation HIV-1 vector in both mouse and human HSCs. Interestingly, the presence or absence of Tat in viral preparations did not influence the transduction efficiency of HIV-1 vectors in human HSCs.
Specificity and cross-reactivity of staphylococcal enterotoxin A monoclonal antibodies with enterotoxins B, C1, D, and E (1986)

The cross-reactivity of monoclonal antibodies produced against staphylococcal enterotoxin A with purified and crude enterotoxins B, C1, D, and E and the specificity of such reactions were evaluated by the indirect enzyme-linked immunosorbent assay and immunoblotting of Western blots (from sodium dodecyl sulfate-polyacrylamide gel electrophoresis) followed by autoradiography. Purified and crude enterotoxins B were also tested with polyclonal antibodies. Specificity of reactivity was demonstrated by immunoblotting of crude enterotoxin A, crude enterotoxin A treated with trypsin, crude enterotoxin E, and also with crude A, B, C1, and D that were pretreated with Sepharose-4B-linked normal rabbit immunoglobulin G to remove protein A. A band corresponding to each staphylococcal enterotoxin was seen with monoclonal antibodies under all conditions tested and also with crude and purified enterotoxin B with two different (rabbit and goat) polyclonal antisera.
Antioxidant and Antifatigue Properties of the Aqueous Extract of <i>Moringa oleifera</i> in Rats Subjected to Forced Swimming Endurance Test (2016)

The effects of the aqueous extract of Moringa oleifera on swimming performance and related biochemical parameters were investigated in male Wistar rats (130-132 g). Four groups of rats (16 per group) were fed a standard laboratory diet and given distilled water, 100, 200, or 400 mg/kg of extract, respectively, for 28 days. On day 28, 8 rats from each group were subjected to the forced swimming test with tail load (10% of body weight). The remaining 8 rats per group were subjected to the 90-minute free swim. Maximum swimming time, glycemia, lactamia, uremia, triglyceridemia, hepatic and muscle glycogen, hematological parameters, and oxidative stress parameters (superoxide dismutase, catalase, reduced glutathione, and malondialdehyde) were measured. Results. M. oleifera extract increased maximum swimming time, blood hemoglobin, blood glucose, and hepatic and muscle glycogen reserves. The extract also increased the activity of antioxidant enzymes and decreased the blood concentrations of malondialdehyde. Furthermore, it decreased blood concentrations of lactate, triglycerides, and urea. In conclusion, the antifatigue properties of M. oleifera extract are demonstrated by its ability to improve body energy stores and tissue antioxidant capacity and to reduce the tissue build-up of lactic acid.
Where is the Tag? (2011)

Radiofrequency identification (RFID) has penetrated logistics, manufacturing, production, ticketing, access control, baggage tagging and various other areas of our daily life [1]. RFID technology has experienced tremendous growth and development since its humble beginnings back in the 1940s [2]. These remarkable technical advances have resulted in enhanced performance and novel application areas, which in turn stimulate new needs and spawn exciting new research initiatives. A current hot research topic in the RFID field is RFID localization.
Development of a virtual teaching pendant system for serial robots based on ROS-I (2017)

The aim of this paper was to present a virtual teaching pendant system for serial industrial robots. Based on Robot Operating System Industrial (ROS-I), the virtual teaching pendant system we designed has the capacity for robot model establishment, direct and inverse kinematics manipulation, as well as motion planning. This virtual teaching pendant system not only contains the main teaching pendant functions but can drive any serial robots in both simulation and real-world. This paper will also highlight the many advantages of this virtual teaching pendant system which can be used as a good teaching tool to help students in understanding robotics. Several demonstrations are made to show the universality and functionality of the virtual teaching pendant system.
Food Security for older people in rural and regional Australia (2012)

Recent research has identified a significant prevalence of malnutrition amongst community-dwelling older Australians. Food insecurity and inadequate nutrition contribute to reduced quality of life and a range of adverse health consequences for older people. An in-depth understanding of the etiology and experience of food insecurity is warranted in order to develop and implement effective policies and service interventions to address food insecurity in this growing sub-group of the population. Although food insecurity in older adults is considered to be worthy of closer attention by researchers, few in-depth qualitative studies have been conducted in Australia. More importantly, current framewords and tools to assess food insecurity in Australia and elsewhere tend to focus on economic, geographic and nutritional factors that can be quantified. These approaches oftend do not consider important social and place-based issues that influence the role and experience of food and eating in older people's lives.
Detection and Classification of Changes in Buildings from Airborne Laser Scanning Data (2013)

Abstract. Building change detection serves to investigate illegal buildings. Illegal built or removed structures, especially those concealed among gable roofs such as dormers, are difficult to track among potentially millions of buildings. Nevertheless, they can be efficiently located in changed areas. An approach is proposed to automatically detect and classify changes in buildings from two epochs of Airborne Laser Scanning Data. Both datasets are classified into water, ground, building, vegetation and undefined objects in advance. After generalization of a 3D surface separation map, we verify changes by making rules on the separation map. Changes belonging to buildings are then classified into roof, wall, dormers, vehicles, construction above roof and undefined objects. As the ALS data has accuracy in strip difference of lower than 5 cm within the same epoch and from different epochs, changes that are larger than 10 cm were detected. Building changes, which areas are larger than 4 m2, are identified as change. By inspection, nearly all changes are detected and approximately 80% changes are correctly classified.
Stabilization of the E* Form Turns Thrombin into an Anticoagulant (2009)

Previous studies have shown that deletion of nine residues in the autolysis loop of thrombin produces a mutant with an anticoagulant propensity of potential clinical relevance, but the molecular origin of the effect has remained unresolved. The x-ray crystal structure of this mutant solved in the free form at 1.55 A resolution reveals an inactive conformation that is practically identical (root mean square deviation of 0.154 A) to the recently identified E* form. The side chain of Trp(215) collapses into the active site by shifting > 10 A from its position in the active E form, and the oxyanion hole is disrupted by a flip of the Glu(192)-Gly(193) peptide bond. This finding confirms the existence of the inactive form E* in essentially the same incarnation as first identified in the structure of the thrombin mutant D102N. In addition, it demonstrates that the anticoagulant profile often caused by a mutation of the thrombin scaffold finds its likely molecular origin in the stabilization of the inactive E* form that is selectively shifted to the active E form upon thrombomodulin and protein C binding.
Backbone-based roadmaps for robot navigation in sensor networks (2008)

In this paper, we propose a distributed path planning method for robot navigation amidst a wireless sensor network. Our method uses communication backbone as a roadmap. In building and maintaining the roadmap, it takes path safety and network longevity into account, and therefore the roadmap adapts to dynamic dangers and evolves over time to increase network longevity. To find a safe path, a navigation field is propagated over the roadmap and the shortest path is computed. Simulation results show that as compared to existing methods our method finds a safe path with less communication cost, and in dense networks it generates smaller roadmaps. We also provide theoretical bounds on the path quality in terms of path length.
Built-in test for RAMs (1988)

A built-in test structure is described that is based on the ATS algorithmic test sequence, which provides the shortest possible test for stuck-at faults in a random-access memory (RAM). An initialization step has been added to ATS that allows the modified procedure to detect bit-rail faults. In the test mode, the memory address register is converted to a count-by-three circuit controlled by a four-latch test sequencer. A simple data-compare circuit is placed on the RAM outputs to detect faults.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Cross-coupling Reaction of Alkyl Halides with Organometallic Reagents Using Transition-Metal Catalysts (2004)

Cross-coupling reaction of organic halides with main group organometallic reagents has now become one of the most practical and reliable methods for constructing C-C bonds in organic synthesis. During the last three decades numbers of such reactions using organic halides bearing C (sp2) -X bond have been developed by the aid of transition-metal catalysts, especially Pd and Ni. On the other hand, the use of alkyl halides in transition-metal catalyzed cross-coupling reaction had not well been developed until quite recently. This is probably due to the low reactivities of alkyl halides toward oxidative addition and also to undesirable facile β-elimination from alkylmetal intermediates. Since several years ago, however, some significant methodological evolutions to overcome these difficulties have been achieved by tuning up the ligands and/or constructing novel catalytic systems based on new mechanisms. This review article summarizes recent progress in transition metal-catalyzed cross-coupling reaction of alkyl halides with organometallic reagents.
T regulatory cells and the control of alloimmunity: from characterisation to clinical application (2010)

T regulatory cells (Treg) play an important role in the induction and maintenance of immunological tolerance. Recent findings in experimental transplant models combined with the development of functional reporter mice have opened new avenues to study Treg biology and their therapeutic potential. In particular, recent advances in understanding Treg function and lineage stability revealed unexpected plasticity of this lineage. Nevertheless, pre-clinical and pilot clinical trials using Treg cells as cellular therapies have been initiated suggesting the safety and feasibility of such treatment.