gebhart/patrepeval huggingface dataset:
Viral detection using DNA functionalized gold filaments (2009)

Early detection of pediatric viruses is critical to effective intervention. A successful clinical tool must have a low detection limit, be simple to use and report results quickly. No current method meets all three of these criteria. In this report, we describe an approach that combines simple, rapid processing and label free detection. The method detects viral RNA using DNA hairpin structures covalently attached to a gold filament. In this design, the gold filament serves both to simplify processing and enable fluorescence detection. The approach was evaluated by assaying for the presence of respiratory syncytial virus (RSV) using the DNA hairpin probe 5' [C6Thiol]TTTTTTTTTTCGACGAAAAATGGGGCAAATACGTCG[CAL] 3' covalently attached to a 5 cm length of a 100 microm diameter gold-clad filament. This sequence was designed to target a portion of the gene end-intergenic gene start signals which is repeated multiple times within the negative-sense genome giving multiple targets for each strand of genomic viral RNA present. The filament functionalized with probes was immersed in a 200 microm capillary tube containing viral RNA, moved to subsequent capillary tubes for rinsing and then scanned for fluorescence. The response curve had a typical sigmoidal shape and plateaued at about 300 plaque forming units (PFU) of viral RNA in 20 microL. The lower limit of detection was determined to be 11.9 PFU. This lower limit of detection was approximately 200 times better than a standard comparison ELISA. The simplicity of the core assay makes this approach attractive for further development as a viral detection platform in a clinical setting.
The cadherin superfamily (2005)

The cadherins are a major class of membrane proteins with prominent roles in cell adhesion, and the regulation of tissue organisation and morphogenesis. The C. elegans genome encodes 13 cadherins, including representatives of the major cadherin sub-types that are conserved between insects and vertebrates: the so-called classic, Fat-like, Flamingo and calsyntenin classes. The function of most of these in C. elegans is still unknown, or poorly understood, mainly because clear loss-of-function mutations have been isolated for only a few. As is true for the cadherin families of other organisms, most is known about classic cadherin function. C. elegans has a single classic cadherin gene, which encodes two isoforms: one predominantly expressed in the nervous system, and the other more broadly expressed in all epithelial cells. The epithelial cadherin-catenin complex appears to be functionally equivalent to that found in Drosophila and vertebrates, and is critically required for embryonic morphogenesis. Mutant phenotypes have also been described for cdh-3 and fmi-1, which encode a Fat-like cadherin, and the C. elegans Flamingo homologue, respectively. cdh-3 mutants display incompletely penetrant defects in the morphogenesis of hyp10, the cell which forms the tip of the tail, and the excretory duct cell; though the mechanistic role of CDH-3 in these processes is not known. FMI-1 is required during neuronal development consistent with the known role of the Drosophila homologue in controlling tissue polarity. Five of the cadherins have no obvious homologues beyond the nematodes, and thus may be phyla-specific.
Molecular Cloning and Characterization of<i>GhlecRK</i>, a Novel Kinase Gene with Lectin-like Domain from<i>Gossypium hirsutum</i> (2004)

A novel gene encoding a lectin-like protein kinase was cloned from the upland cotton (Gossypium hirsutum) through cDNA library screening. This gene (named as Ghlecrk; GenBank accession number: AY487461) had a total length of 2233bp with an open reading frame of 1926bp, and encoded a predicted polypeptide of 641 amino acids with a molecular weight of 71.16kDa. The GhLecRK protein shared 73, 65, 64 and 59% identity with other lectin-like kinase proteins isolated from A. thaliana (At3g53810, At2g37710, At3g55550) and Populus nigra (PnLPK) at amino acid level, respectively. Southern blot analysis showed that GhLecRK belonged to a multi-copy gene family. Expression patterns revealed that GhLecRK was enriched in the developing boll (six days post anthesis, 6DPA) and shoot, but low in the root and stem and no expression in the leaf. The domains analysis showed that GhlecRK protein possessed many activating sites/domains including ATP-binding sites, a transmembrane region, a lectin-like domain and a kinase domain. These results indicate that GhlecRK is a lectin-like membrane protein that may play an important role in the phase of fiber development.
Invitation au vol (1958)

Huile sur toile de 1958 par Victor Brauner.
Une image, une notice descriptive, une bibliographie.
Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product (1989)

The gene coding for the enzyme 2,3-dihydroxybenzoate-AMP ligase (2,3DHB-AMP ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli. The protein is an alpha 2 dimer with subunit molecular mass of 59 kDa. The enzyme catalyzes the exchange of [32P]pyrophosphate with ATP, dependent upon aromatic substrate with a turnover number of 340 min-1. The enzyme also releases pyrophosphate upon incubation with 2,3-dihydroxybenzoic acid and ATP; an initial burst corresponding to 0.7 nmol of pyrophosphate released per nanomole of enzyme is followed by a slower, continuous release with a turnover number of 0.41 min-1. The 1000-fold difference in rates observed between ATP-pyrophosphate exchange and continuous pyrophosphate release, as well as the close to stoichiometric amount of pyrophosphate released, suggests that intermediates are accumulating on the enzyme surface. Such intermediates have been observed and correspond to enzyme-bond (2,3-dihydroxybenzoyl)adenylate product.
Report of Subcommittee B on Recommendations of Referees (1953)

Journal Article Report of Subcommittee B on Recommendations of Referees Frank H Wiley, Frank H Wiley Chairman Division of Pharmaceutical Chemistry, Food and Drug Administration, Federal Security Agency, Washington, D. C ) Search for other works by this author on: Oxford Academic Google Scholar H J Fisher, H J Fisher Search for other works by this author on: Oxford Academic Google Scholar G Robert Clark G Robert Clark Search for other works by this author on: Oxford Academic Google Scholar Journal of Association of Official Agricultural Chemists, Volume 36, Issue 1, 1 February 1953, Pages 52–54, https://doi.org/10.1093/jaoac/36.1.52 Published: 27 January 2020
Evaluation of the sustained release properties of Eudragit RS, RL and S (acrylic resins) microcapsules containing ketoprofen in beagle dogs˚ (1988)

Eudragit RS, RS-RL, RL and S microcapsules containing ketoprofen were prepared by the solvent evaporation process in oil phase. The sustained release effect of these microcapsules and Oruvail, the representative commercial product of ketoprofen, was evaluated by the pH shift dissolution method and in beagle dogs, respectively. The dissolution patterns of ketoprofen from Eudragit RS, RS-RL and RL microcapsules were independent of the pH of the dissolution medium, and its dissolution rate increased with increasing content of ketoprofen in microcapsules. But the dissolution pattern of ketoprofen from Eudragit S microcapsules and Oruvail was found to depend on the pH of the dissolution medium. The rank order of the dissolution rate of ketoprofen from Eudragit RS, RS-RL and RL microcapsules containing 30 and 40 per cent (w/w) ketoprofen was sufficiently clear as to enable prediction of the relative bioavailability of ketoprofen from these microcapsules. In vivo evaluation using beagle dogs, sustained release effects of Eudragit RL and Eudragit S microcapsules containing 30 per cent (w/w) ketoprofen and Eudragit RS-RL microcapsules containing 40 per cent (w/w) ketoprofen were almost the same as or slightly superior to that of Oruvail.
A comparative study of two simulation methods for thermal analysis of power electronic devices (2003)

The authors compare two methods applied to 3D temperature map computation of power hybrid circuits. The first is the conventional FE method, and the second, so-called thermal influence coefficient method (TIC), is a specific procedure devised for fast temperature computations of hybrid power circuits. The numerical methods designed for true 3D thermal analyses have been implemented in programs running on PC-AT microcomputers.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Cross-sectional high-resolution transmission electron microscopy study of the structures of carbon nanotubes (1995)

Abstract The structures of carbon nanotubes are investigated by cross-sectional high-resolution transmission electron microscopy. Some results for longitudinal sections are also included. No examples of perfect seamless tubes or of “scroll-type” Archimedean spirals are found; in each case examined, papier m[acaron]hé-like aggregates of graphene sheets occur, each of finite extent. There is a strong tendency for the structures to relax, locally at least, towards the three-dimensional structure of graphite. Thus polygonal rather than circular or spherical geometries predominate. Some comments are offered concerning a realistic model for the structures, as well as for understanding the electronic properties of multilayer carbon nanotubules. It is proposed that our specimens grew in an atmosphere rich in single graphene sheets, rather than C60 cages.
Individual Monetary Incentives (2001)

Abstract Studies in three thematic lines of research have manipulated parameters of individual monetary incentive systems to determine whether those parameters were functionally related to performance. Studies have examined: (a) the size of the percentage of total pay and base pay earned in incentive pay; (b) various ratio schedules of monetary reinforcement; and (c) linear, accelerating, and decelerating piece rate pay. The review revealed that individual monetary incentives plus feedback improved performance in comparison to hourly pay plus feedback in studies in all three thematic research lines. However, performance levels were not functionally related to (a) the size of the percentage of total pay or base pay earned in incentive pay for percentages that ranged from 3% to 100% of a person's total pay and base pay; (b) the per piece incentive amount; (c) the amount earned in total pay or total incentive pay; (d) the ratio schedule of delivery for CRF, FR3, VR2, VR3, and VR4 schedules; or (e) linear, accelerating, or decelerating piece rate pay. Taken together, the data suggest that, at least for the parameters investigated to date, the most critical determinant of performance is the ratio schedule contingency between performance and pay; that is, a relationship in which individuals earn a specified amount of money for the number of work units they complete. They also suggest that once a ratio relationship exists, variations in the parameters of individual monetary incentive systems may not greatly affect performance. Relatively few studies, however, have been conducted and further research is required. Key Words: Individual monetary incentivesliterature reviewperformance-based monetary incentivesproportion of monetary incentive pay to wage-based pay
Fluid shear stress differentially modulates expression of genes encoding basic fibroblast growth factor and platelet-derived growth factor B chain in vascular endothelium. (1993)

Fluid shear stress has been shown to be an important regulator of vascular structure and function through its effect on the endothelial cell. We have explored the effect of shear stress on the expression of the heparin-binding growth factors platelet-derived growth factor B chain (PDGF-B) and basic fibroblast growth factor (bFGF) in bovine aortic endothelial cells using a purpose-built cone-plate viscometer. Using morphometric analysis, we have mimicked the endothelial cell shape changes encountered in vivo in response to shear stress and correlated these with changes in gene expression. Steady laminar shear stress of 15 and 36 dyn/cm2 both resulted in endothelial cell shape change, but the higher shear stress induced greater and more uniform alignment in the direction of flow and nuclear protrusion after 24 h. Steady laminar shear stress of both 15 and 36 dyn/cm2 induced a significant 3.9- and 4.2-fold decrease, respectively, in PDGF-B mRNA at 9 h. In contrast, steady laminar shear of 15 dyn/cm2 induced a mild and transient 1.5-fold increase in bFGF mRNA while shear of 36 dyn/cm2 induced a significant 4.8-fold increase at 6 h of shear which remained at 2.9-fold at 9 h. Pulsatile and turbulent shear stress showed the same effect as steady laminar shear stress (all at 15 dyn/cm2 time-average magnitude) on PDGF-B and bFGF mRNA content. Cyclic stretch (20% strain, 20/min) of cells grown on silicone substrate did not significantly affect either PDGF-B or bFGF mRNA levels. These results suggest that expression of each peptide growth factor gene is differentially regulated by fluid shear stress in the vascular endothelial cell. These results may have implications on vascular structure and function in response to hemodynamic forces and present a model for the study of transduction of mechanical stimuli into altered gene expression.
<i>ELONGATED UPPERMOST INTERNODE</i> Encodes a Cytochrome P450 Monooxygenase That Epoxidizes Gibberellins in a Novel Deactivation Reaction in Rice (2006)

The recessive tall rice (Oryza sativa) mutant elongated uppermost internode (eui) is morphologically normal until its final internode elongates drastically at the heading stage. The stage-specific developmental effect of the eui mutation has been used in the breeding of hybrid rice to improve the performance of heading in male sterile cultivars. We found that the eui mutant accumulated exceptionally large amounts of biologically active gibberellins (GAs) in the uppermost internode. Map-based cloning revealed that the Eui gene encodes a previously uncharacterized cytochrome P450 monooxygenase, CYP714D1. Using heterologous expression in yeast, we found that EUI catalyzed 16alpha,17-epoxidation of non-13-hydroxylated GAs. Consistent with the tall and dwarfed phenotypes of the eui mutant and Eui-overexpressing transgenic plants, respectively, 16alpha,17-epoxidation reduced the biological activity of GA(4) in rice, demonstrating that EUI functions as a GA-deactivating enzyme. Expression of Eui appeared tightly regulated during plant development, in agreement with the stage-specific eui phenotypes. These results indicate the existence of an unrecognized pathway for GA deactivation by EUI during the growth of wild-type internodes. The identification of Eui as a GA catabolism gene provides additional evidence that the GA metabolism pathway is a useful target for increasing the agronomic value of crops.
Face Authentication using Multi-radius LBP Matching of Individual Major Blocks in Mobile Environment (2013)

본 논문에서는 모바일 환경에서의 얼굴인증을 위해 개인별 주요 블록에 대한 LBP 매칭 기반의 새로운 방법을 제안한다. 주어진 사진으로부터 개인별 주요 블록을 구성하기 위하여 유사도가 높은 블록을 찾아 블록별 확률 분포를 계산하고 임계값을 적용하여 사람마다 다른 블록의 개수를 얼굴인증에 사용하도록 한다. 그리고 단일 반경 LBP 기반 얼굴인증 방법의 성능 향상을 위하여 다중 반경 LBP 히스토그램을 이용하여 개인별 주요 블록을 결정한다. 실험 결과 다중 반경 LBP 히스토그램을 이용함으로써 단일 반경 LBP 히스토그램만 사용하여 얼굴인증을 수행할 때보다 타인의 인증을 수락하는 오인수락율을 줄일 수 있었다. 또한, 기존 방법과의 성능 비교에서 제안한 방법이 블록의 개수는 기존 방법의 44.59%만 사용하면서 인증 오류율은 평균 7.72% 낮은 결과를 얻었다. In this paper, we propose a novel face authentication method based on LBP matching of individual major blocks in mobile environment. In order to construct individual major blocks from photos, we find the blocks that have the highest similarity and use different numbers of blocks depending on the probability distribution by applying threshold. And, we use multi-radius LBP histograms in the determination of individual major blocks to improve performance of generic LBP histogram based approach. By using the multi-radius LBP histograms in face authentication, we can successfully reduce the false acceptance rate compare to the previous methods. Also, we can see that the proposed method shows low error rate about 7.72% compare to the pervious method in spite of use small number of blocks about 44.59% only.
연구논문 : 철코팅 불가사리를 이용한 중금속 제거 특성 (2004)

The ability of iron coated starfish (ICS) to adsorb heavy metals from aqueous solution was studied with variation of factors, such as starfish dose, pH, single solute, and mixture. It was found that heavy metals removal capacity was significantly increased through iron-coating. The results show that 72.2%, 46.8%, and 22.5% of Pb, Cu, and Mn were removed by starfish, while 100% of the three metals were removed by ICS after 150 minutes. Competitive adsorption of starfish for Pb, Cu, and Mn from water was investigated by batch experiment. Starfish has the greatest affinity for Pb compared to Cu and Mn at a concentration of 100 mg/L of each metals and an interval of pH=4.5~8. Freundlich and Langmuir adsorption isotherms were utilized to describe the adsorption reaction. Adsorption isotherm results indicated that the adsorption capacity of ICS for Cu was 0.0537 mg/g and that of starfish for Cu was 0.0327 mg/g. This study shows that starfish can be converted to a useful adsorbent for heavy metals removal.
Synthesis of indolin-2-ones (oxindoles) related to mitomycin A (1988)

Oxindoles (Indolin-2-ones) containing the functionality of the first two rings of the mitomycin A structure have been synthesized. Attempts to employ the oxindole grouping to construct the third ring were unsuccessful because of the resistance of the oxindole group or its derivatives towards nucleophilic attack. An unusual methylation of 3-methyl-5-nitrobenzene-1,2,4-triol to give the (E)-3,6-dihydroxy-2-methyl-1,4-benzoquinone 4-methoxyimine N-oxide is noted. A novel indole formation is described.
Bridge Inspections Come of Age (2017)

Arising from the tragic collapse of a West Virginia bridge 50 years ago this month, the National Bridge Inspection Program has helped keep bridges in the United States safe for motorists for decades. The standards and procedures for bridge inspections have changed dramatically over the years, and technology is fueling even greater transformations. But experts say it will be some time before robots and drones take the place of highly trained, experienced inspectors.
Effect of Passive Modes in Traveling-Wave Tubes (1948)

As the beam current in a traveling-wave tube is increased, the local fields due to the bunched beam become appreciable compared with the fields propagating along the circuit. The effect is to reduce gain, to increase the electron speed for optimum gain, to introduce a lower limit to the range of electron speeds for which gain is obtained, and to change the initial loss.
Gurney Flap Scaling for Optimum Lift-to-Drag Ratio (1997)

This note aims at providing evidence that there exists a flow-based scaling for the Gurney flap heights that yield an increase in lift-to-drag performance compared with the baseline airfoil at the same angle of attack (beneficial Gurney flaps). The results presented here, support this statement and further suggest that the boundary-layer thickness δ, measured at the trailing edge on the pressure side of the baseline airfoil, is not only an appropriate flow-based normalization for the flap height but is also a proper order of magnitude for the flap height providing the largest increase in the lift-to-drag ratio (optimum Gurney flap)
Preparation and Properties of Resacetophenone-Thiourea-Trioxane Polymers (1991)

Polymers (RTT') were synmthesized by the condensation of resacetophe-none (R) with thiourea (T) and trioxane (T') in the presence of 2 Mu HC1 or 2 M H 2 S0 4 as a catalyst and varying molar proportions of reactants. The polymers were characterized by elemental analyses and IR spectral studies. The number average molecular weights of polymers were determined by vapour pressure osmometry and non-aqueous conductometric titrations in pyridine. Viscosity measurements were carried out in DMF Thermogravimetric measurements of the polymers have also been carried out.
A Correlation Studies of Indoor Environmental Quality(IEQ) Towards Productive Workplace (2011)

Productivity is one of the major contributions to build the economic growth in every country. Human performance and working environment is related to each other and have a major impact on the work efficiency and production output. Nowadays, most of the offices are fully equipped with mechanical ventilation and fully air-conditioned. Office building occupants spend almost 12 hours daily indoor and that makes good indoor environment very important due to achieve workers satisfaction and more comfortable. The objective of this paper is to observe the correlation of IEQ and productivity in working place. It is believed that this study have been able to provide a better understanding. It is hoped that this useful findings would serve as a reference in the future to the building industry, academicians and also public in sustaining better indoor environmental quality.
A Mutant RNA Polymerase Reveals a Kinetic Mechanism for the Switch between Nonproductive Stuttering Synthesis and Productive Initiation during Promoter Clearance (1996)

During transcription initiation from galP2, one of the two promoters of the Escherichia coli galactose operon with an initially transcribed sequence of pppAUUUC, RNA polymerase (RNAP) is known to engage nonproductive stuttering synthesis, which is sensitive to the concentration of UTP. This study examines the effect of this nonproductive synthesis on promoter clearance and determines other parameters that might affect stuttering synthesis by analyzing a mutant RNAP, RpoB3449, that has altered its function at this process at galP2. RpoB3449 has dramatically diminished stuttering synthesis, and consequently, it has increased the rate of productive initiation due to its enhanced rate of promoter clearance of galP2 compared with wild-type RNAP. Thus, a direct linkage between promoter clearance and productive transcription is demonstrated. The mechanism by which the mutant RNAP has altered the switch between nonproductive stuttering synthesis and productive initiation during promoter clearance is studied. Apparently, RpoB3449 has increased its efficiency in incorporating CTP at the +5 position of the galP2 transcript leading to its reduced stuttering synthesis, indicating that the rate of an RNAP incorporating the CTP after a stretch of uridine residues is important for promoter clearance at galP2. Because RpoB3449 demonstrates "wild-type" stuttering synthesis at the mutant galP2 promoter, which contains the 6 residue at the +5 position, it indicates that the mutant RNAP has altered in binding CTP at this context. Further experiments indicate that it is the +5 position per se of the galP2 sequence rather than a particular nucleotide at that position that is critical in determining the switch between the two alternate pathways during transcription initiation. A checkpoint model for the switch between nonproductive and productive initiations during promoter clearance is discussed.