gebhart/patrepeval huggingface dataset:
Metnase Mediates Resistance to Topoisomerase II Inhibitors in Breast Cancer Cells (2009)

DNA replication produces tangled, or catenated, chromatids, that must be decatenated prior to mitosis or catastrophic genomic damage will occur. Topoisomerase IIalpha (Topo IIalpha) is the primary decatenating enzyme. Cells monitor catenation status and activate decatenation checkpoints when decatenation is incomplete, which occurs when Topo IIalpha is inhibited by chemotherapy agents such as the anthracyclines and epididophyllotoxins. We recently demonstrated that the DNA repair component Metnase (also called SETMAR) enhances Topo IIalpha-mediated decatenation, and hypothesized that Metnase could mediate resistance to Topo IIalpha inhibitors. Here we show that Metnase interacts with Topo IIalpha in breast cancer cells, and that reducing Metnase expression significantly increases metaphase decatenation checkpoint arrest. Repression of Metnase sensitizes breast cancer cells to Topo IIalpha inhibitors, and directly blocks the inhibitory effect of the anthracycline adriamycin on Topo IIalpha-mediated decatenation in vitro. Thus, Metnase may mediate resistance to Topo IIalpha inhibitors, and could be a biomarker for clinical sensitivity to anthracyclines. Metnase could also become an important target for combination chemotherapy with current Topo IIalpha inhibitors, specifically in anthracycline-resistant breast cancer.
A current conveyor II realized by Mosfets and its application to switched capacitor filters (1986)

Abstract From the viewpoint of intergration, a MOSFET configuration is superior to the previous current conveyors (CC) consisting of an operational amplifier and/or bipolar transistors In this paper, a current conveyor II (CC II) is composed of an operational amplifier and MOSFETs. It was clarified that the current transfer ratio from X terminal (input) to Z terminal (output) of CC II can be put to variable K i ., not to 1, because the MOSFET is a voltage‐controlled device. As a case of application, a switched capacitor (SC) integrator is realized with our proposed CC II and its transfer characteristic is studied theroretically and experimentally. Then, by applying this SC integrator to a Biquard‐type bandpass switched capacitor filter (SCF), it turned out that the electric capacitor of the SCF can be reduced largely and the SCF integration is superior in this regard.
Specificity of ligand binding in a buried nonpolar cavity of T4 lysozyme: Linkage of dynamics and structural plasticity (1995)

To better understand the role of shape complementarity in ligand binding and protein core interactions, the structures have been determined of a set of ligands bound within a cavity-containing mutant of T4 lysozyme. The interior cavity is seen to consist of two parts that respond very differently to the binding of ligands. First, there is a relatively rigid region that does not relax significantly upon binding any ligand. Second, there is a more flexible region that moves to various extents in response to binding the different ligands. The part of the binding site that remains rigid is characterized by low temperature factors and strong protection from hydrogen exchange. This part of the site appears to be primarily responsible for discriminating between ligands of different shape (i.e., for determining specificity). The more flexible region, characterized by relatively high temperature factors and weak protection from hydrogen exchange, allows some promiscuity in binding by undergoing variable amounts of deformation at essentially the same energetic cost. This linkage between the dynamic information represented by crystallographic temperature factors and hydrogen-exchange behavior on the one hand, and structural plasticity in response to ligand binding on the other hand, suggests a way to improve our understanding of steric interactions in protein cores and protein-ligand binding sites. Ligand design and packing algorithms might take advantage of this information, requiring complementary interactions where the protein is rigid and allowing some overlap in regions where the protein is flexible.
Scalable Big Data Architecture (2015)

This book highlights the different types of data architecture and illustrates the many possibilities hidden behind the term "Big Data", from the usage of No-SQL databases to the deployment of stream a
Identification and isolation of transcribed human X chromosome DNA sequences (1983)

A human X chromosome specific phage library has been used as a source of X-specific genomic DNA clones which hybridize with cellular RNA. Random cDNA clones were mapped for X chromosome sequence localization and 8 were identified as hybridizing to X chromosome Hind III fragments. All eight also hybridized with autosomal Hind III fragments. The X chromosome genomic sequences corresponding to two of these cDNA clones were isolated from a phage library constructed with the Hind III endonuclease digest products of X enriched DNA. One genomic DNA segment, localized to the short area of the X, shared sequence homology with at least one region of the human Y chromosome. The methodology developed represents a rapid means to obtain a specific genomic DNA clone from a single chromosome when multiple different genomic loci homologous to an expressed DNA sequence exist.
Streamer noise reduction with manufacturing techniques (1989)

Results of marine geophysical tests show that a highamplitude low-frequency noise wave is propagated through the cable from section to section at a relatively high velocity but is propagated within each section at a relatively low veIocity . This noise wave has been generally referred to as the 'bulge wave' or 'cable jerk'. Results of early tests reveal characteristics about the bulge wave that can be utilized in the streamer design to effectively attenuate this noise.
iRFP is a sensitive marker for cell number and tumor growth in high-throughput systems (2013)

GFP and luciferase are used extensively as markers both in vitro and in vivo although both have limitations. The utility of GFP fluorescence is restricted by high background signal and poor tissue penetrance. Luciferase throughput is limited in vitro by the requirement for cell lysis, while in vivo, luciferase readout is complicated by the need for substrate injection and the dependence on endogenous ATP. Here we show that near-infrared fluorescent protein in combination with widely available near-infrared scanners overcomes these obstacles and allows for the accurate determination of cell number in vitro and tumor growth in vivo in a high-throughput manner and at negligible per-well costs. This system represents a significant advance in tracking cell proliferation in tissue culture as well as in animals, with widespread applications in cell biology.
Rootstock effect on olive: Influence on tree growth is found to vary with the scion variety in tests conducted at Winters, Corning, and Lindsay (1958)

In experimental olive plantings—using three scion varieties and 12 different rootstocks—the principal rootstock effect on the scion variety was in tree vigor. However, any rootstock influence noted in a given scion-rootstock combination did not necessarily hold true for another scion variety on the same rootstock.
Anodic Dissolution and Passivation of Titanium in Acidic Media: III . Chloride Solutions (1979)

The electrochemical behavior of titanium in deoxygenated acidic chloride solutions, with and without additions of Ti(III) and Ti(IV) ions, has been determined and compared with that observed in acidic sulfate media. A monolayer mechanism has been developed for the active‐state dissolution and passivation of titanium in acidic media. According to this mechanism, the metal is oxidized in a sequence of one‐electron charge‐transfer reactions which results in the formation of adsorbed reaction intermediates corresponding to each of the relevant valence states of titanium (+1, +2, +3, +4) and which leads to Ti(III) ions in solution in the active and active‐passive transition regions. The mechanism is in quantitative agreement with the experimental results for both chloride and sulfate media. In acidic chloride solutions, as well as in acidic sulfate solutions, the rates of oxidation at a passive titanium surface of Ti(III) ions in solution to Ti(IV) and of reduction at an active‐state surface of Ti(IV) ions in solution to Ti(III) are directly proportional to the concentrations of Ti(III) and Ti(IV), respectively. The reduction of Ti(IV) at an active surface is responsible for the fact that, at a critical concentration of Ti(IV), an active‐state surface passivates. In localized corrosion systems, active and passive surfaces are in simultaneous contact with the electrolyte within the occluded cell, and the aforementioned oxidation and reduction reactions serve to couple the active‐state and passive‐state electrochemical systems. Such electrolyte‐coupled active‐passive systems are capable of generating the critical concentration of Ti(IV) required to passivate the active‐state surface, a fact which explains random spontaneous cessation of localized corrosion (self‐healing). The critical concentration of Ti(IV) is much greater in chloride solutions than in sulfate media and takes much longer to attain. Consequently, the halide ion functions as a promoter of localized corrosion.
Native <i>Escherichia coli</i> SufA, Coexpressed with SufBCDSE, Purifies as a [2Fe−2S] Protein and Acts as an Fe−S Transporter to Fe−S Target Enzymes (2009)

Iron-sulfur (Fe-S) clusters are versatile biological cofactors that require biosynthetic systems in vivo to be assembled. In Escherichia coli, the Isc (iscRSUA-hscBA-fdx-iscX) and Suf (sufABCDSE) pathways fulfill this function. Despite extensive biochemical and genetic analysis of these two pathways, the physiological function of the A-type proteins of each pathway (IscA and SufA) is still unclear. Studies conducted in vitro suggest two possible functions for A-type proteins, as Fe-S scaffold/transfer proteins or as iron donors during cluster assembly. To resolve this issue, SufA was coexpressed in vivo with its cognate partner proteins from the suf operon, SufBCDSE. Native SufA purified anaerobically using this approach was unambiguously demonstrated to be a [2Fe-2S] protein by biochemical analysis and UV-vis, Mossbauer, resonance Raman, and EPR spectroscopy. Furthermore, native [2Fe-2S] SufA can transfer its Fe-S cluster to both [2Fe-2S] and [4Fe-4S] apoproteins. These results clearly show that A-type proteins form Fe-S clusters in vivo and are competent to function as Fe-S transfer proteins as purified. This study resolves the contradictory results from previous in vitro studies and demonstrates the critical importance of providing in vivo partner proteins during protein overexpression to allow correct biochemical maturation of metalloproteins.
Essays on Mobile Advertising and Commerce (2009)

The mobile industry holds the promise of increasing connectivity, productivity, and entertainment as mobile devices become ever more ubiquitous and powerful. Many of the market structures that enable mobile commerce are still under rapid development and afford us opportunities to ask new questions about market design. This thesis examines three such markets and mechanisms that drive advertising platforms and application stores for mobile commerce. In the first essay, I analyze a mobile web advertising auction that employs a proportional allocation rule in which advertisements are shown with frequencies proportional to the bids. Proportional allocation is used to address the space constraints of the mobile environment and the accompanying ad fatigue. I show that the second-price
Depression Increasingly Predicts Mortality in the Course of Congestive Heart Failure (2004)

Abstract Background Congestive heart failure (CHF) is frequently associated with depression. However, the impact of depression on prognosis has not yet been sufficiently established. Aims To prospectively investigate the influence of depression on mortality in patients with CHF. Methods In 209 CHF patients depression was assessed by the Hospital Anxiety and Depression Scale (HADS-D). Results Compared to survivors (n=164), non-survivors (n=45) were characterized by a higher New York Heart Association (NYHA) functional class (2.8±0.7 vs. 2.5±0.6), and a lower left ventricular ejection fraction (LVEF) (18±8 vs. 23±10%) and peakVO2 (13.1±4.5 vs. 15.4±5.2 ml/kg/min) at baseline. Furthermore, non-survivors had a higher depression score (7.5±4.0 vs. 6.1±4.3) (all P&lt;0.05). After a mean follow-up of 24.8 months the depression score was identified as a significant indicator of mortality (P&lt;0.01). In multivariate analysis the depression score predicted mortality independent from NYHA functional class, LVEF and peakVO2. Combination of depression score, LVEF and peakVO2 allowed for a better risk stratification than combination of LVEF and peakVO2 alone. The risk ratio for mortality in patients with an elevated depression score (i.e. above the median) rose over time to 8.2 after 30 months (CI 2.62−25.84). Conclusions The depression score predicts mortality independent of somatic parameters in CHF patients not treated for depression. Its prognostic power increases over time and should, thus, be accounted for in risk stratification and therapy.
High-Pressure Inactivation of Hepatitis A Virus within Oysters (2005)

Previous results demonstrated that hepatitis A virus (HAV) could be inactivated by high hydrostatic pressure (HHP) (D. H. Kingsley, D. Hoover, E. Papafragkou, and G. P. Richards, J. Food Prot. 65:1605-1609, 2002); however, direct evaluation of HAV inactivation within contaminated oysters was not performed. In this study, we report confirmation that HAV within contaminated shellfish is inactivated by HHP. Shellfish were initially contaminated with HAV by using a flowthrough system. PFU reductions of >1, >2, and >3 log(10) were observed for 1-min treatments at 350, 375, and 400 megapascals, respectively, within a temperature range of 8.7 to 10.3 degrees C. Bioconcentration of nearly 6 log(10) PFU of HAV per oyster was achieved under simulated natural conditions. These results suggest that HHP treatment of raw shellfish will be a viable strategy for the reduction of infectious HAV.
Person-to-Person Asymptomatic Infection of Severe Fever with Thrombocytopenia Syndrome Virus through Blood Contact (2014)

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease recently discovered in northeastern and central China that is caused by a novel bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). Humans are primarily infected through tick bites. Four previous reports have discussed SFTS infection from person to person, all cases of which were symptomatic. In this report, we analysed the epidemiological and clinical data for a cluster of cases, including one case of secondary-asymptomatic infection, and review the literature regarding SFTSV transmission from person to person. We conclude that SFTSV caused the asymptomatic infections via person-to-person contact with infected blood.
DETECTING REJECTION AFTER MOUSE ISLET TRANSPLANTATION UTILIZING ISLET PROTEIN-STIMULATED ELISPOT (2010)

Toso, C.; Pawlick, R.; Edgar, R.; Lacotte, S.; Davis, J.; Mc Call, M.; Berney, T.; Shapiro, J. Author Information
Mergers in South African higher education: theorising change in transitional contexts (2003)

Drawing on a study of five merger cases in recent South African higher education, this article examines why in each case, the mergers proceeded despite intense opposition from the entities affected and in a form and manner different from that envisaged by their state designers. It considers too, the inadequacies of existing merger theories to explain these two factors and draws on contingency theory to show how the merger outcomes were the product of a complex interplay between governmental macro-politics and institutional micro-politics in a context of political transition. It also exposes the assumption that policy implementation is a rational process in which institutional practice mirrors the formal intentions of government planners, arguing that the merger process in South Africa has to date been marked by behaviour and action that has been both irrational and incoherent as well as not necessarily in the interests of the higher educational process.
A graph-based joint bilateral approach for depth enhancement (2014)

Depth images are often presented at a lower spatial resolution, either due to limitations in the acquisition of the depth or to increase compression efficiency. As a result, upsampling low-resolution depth images to a higher spatial resolution is typically required prior to depth image based rendering. In this paper, depth enhancement and up-sampling techniques are proposed using a graph-based formulation. In one scheme, the depth is first upsampled using a conventional method, then followed by a graph-based joint bilateral filtering to enhance edges and reduce noise. A second scheme avoids the two-step processing and upsamples the depth directly using the proposed graph-based joint bilateral upsampling. Both filtering and interpolation problems are formulated as regularization problems and the solutions are different from conventional approaches. Further, we also studied operations on different graph structures such as star graph and 8-connected graph. Experimental results show that the proposed methods produce slightly more accurate depth at the full resolution with improved rendering quality of intermediate views.
On-Line Subtraction of the Cardiac Activity from the Esophageal Electromyogram of the Diaphragm (1968)

A system is described whereby the integrated electrocardiogram is automatically subtracted from the total integrated electrical activity recorded in the esophagus. By this method, a measure of the electrical activity in the diaphragm is obtained, and the output from the respiratory center can be studied independently of mechanical alterations of chest wall and lungs.
Legibility of Words Rendered Using Cleartype™ (2002)

ClearType™ is a software technique that uses sub-pixel addressing in an effort to increase the readability of digitized text. To evaluate whether ClearType™ improves the legibility of words relative to aliased text, 24 students performed a lexical decision task that involved brief exposures to 280 words and 280 non-words at different retinal eccentricities. Participants were significantly more accurate at identifying words with ClearType™ than without ClearType™. Accuracy was also higher with non-italicized text and when the text was centered around the fixation point. Response times did not significantly vary across these same variables. Thus ClearType™ offers a significant improvement in word legibility while not adding to the cost of display hardware.
Methanol Electro-Oxidation on Well-Characterized Pt-Ru Alloys - Temperature Effects (1994)

A Summary Report to The Electrochemical Society for the 1993 Joseph W. Richards Summer Research Fellowship.
A DC-powered Josephson logic family that uses hybrid unlatching flip-flop logic elements (Huffles) (1995)

This paper describes the development of a dc-powered Josephson logic family that uses hybrid unlatching flip-flop logic elements (Huffles). The Huffle circuit used in this study is modified by adding a parallel resistor to the original Hebard-type Huffle circuit. Analysis of the circuit's operation shows that the undesirable hung-up phenomena are prevented by this modification. Based on the result of the analysis, the circuit's parameters are derived and a typical operating margin of /spl plusmn/26% is obtained. Besides AND/OR operations using a threshold logic operation, two-input exclusive OR (XOR), two-input multiplexor (MUX), and three-input majority (MAJ) operations are realized using a Huffle gate in which 2-Josephson-interferometers (2JI) in the standard Huffle gate are replaced by stacked-2JI's. Thus, a Huffle logic family, formed from NOT, AND, OR, XOR, MUX, MAJ, and flip-flop (FF), are constructed. By using this Huffle logic family, a 6-b arithmetic logic operating unit (ALU), a 6-b analog-to-digital converter (ADC), and a 6-b gray-to-binary converter (GBC) have been successfully operated. During high-speed testing, a 1-b comparator was operated up to an input bandwidth of 6 GHz.
Current-Spin Coupling for Ferromagnetic Domain Walls in Fine Wires (2005)

The coupling between a current and a domain wall is examined. In the presence of a finite current and in the absence of a potential which breaks the translational symmetry, there is a perfect transfer of angular momentum from the conduction electrons to the wall. As a result, the ground state is in uniform motion and this remains the case even when relaxation is included. This is described by, appropriately modified, Landau-Lifshitz-Gilbert equations. The results for a simple pinning model are compared with experiment.
Image processing, a new diagnostic tool (1994)

The San Antonio Air Logistics Center (SA-ALC) Automatic Test Systems Division's Advanced Diagnostics and Technology Insertion Center (ADTIC) is exploring the addition of a non-intrusive diagnostics capability to existing Automatic Test Systems (ATS) utilizing various sensor technologies. The diagnostic techniques under development are expected to allow for more efficient fault detection and isolation than traditional ATS. This paper summarizes findings to date and discusses the integration of these technologies should they prove viable.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Soybean Oil Based Greases:  Influence of Composition on Thermo-oxidative and Tribochemical Behavior (2005)

The biodegradable properties and lubricating ability of greases depend on both the base oil and the thickener. Mineral oils are the most widely used lubricant base fluids due to their inherent lubricity and low cost, but recent environmental awareness has forced consideration of the use of biodegradable fluids such as vegetable oils and certain synthetic fluids in grease formulations. This study presents data on the thermo-oxidation behavior and tribology of biodegradable greases formulated with soybean oil and different compositions of metal soap thickener. The composition of thickener has been varied by using fatty acids with different degrees of unsaturation and fatty acids of different chain lengths. The improvement of thermo-oxidation and tribological properties as a result of changing thickener/base oil ratio and the antioxidative effect of some suitable additives have also been evaluated.
Secretion of novel and homologous neutrophil-activating peptides by LPS-stimulated human endothelial cells. (1989)

Human umbilical vein endothelial cells in culture produce two chemotactic polypeptides when stimulated with LPS. The chemotactic factors could be purified to apparent homogeneity by HPLC techniques and were identified as 7.5-kDa and 15-kDa polypeptides by SDS-PAGE under nonreducing conditions. Both factors are potent chemotaxins for human neutrophils demonstrating half-maximal chemotaxis at 2 ng/ml and g ng/ml, respectively. In addition both peptides elicited release of azurophilic granule constituents when neutrophils were pretreated with cytochalasin B. Cross-desensitization experiments by using human neutrophils revealed cross-reactivities between both chemotaxins, not, however, with C5a or FMLP, indicating that both endothelial cell-derived neutrophil activating peptides (ENAP) are homologous. In addition, the 7.5-kDa factor (beta-ENAP) proved to be the quantitatively dominating and more potent chemotaxin as compared to the 15 kDa factor (alpha-ENAP). beta-ENAP shows biochemical and biologic similarities to monocyte- and lymphocyte-derived neutrophil-activating peptides MONAP and LYNAP, which recently were purified and sequenced.