gebhart/patrepeval huggingface dataset:
Combined analysis of intracellular signalling and immunophenotype of human peripheral blood basophils by flow cytometry: a proof of concept (2007)

This study is the first to provide evidence for a combined analysis of basophilic intracellular signalling and immunophenotyping by flow cytometry. Owing to its technical simplicity, the low number of cells required and rapid analysis, the technique seems promising for use in the clinic as a diagnostic tool or to monitor therapy.
Cell cycle kinetics in corneal endothelium from old and young donors. (2000)

Regardless of donor age, corneal endothelial cells can enter and complete the cell cycle. In the presence of fetal bovine serum and FGF, cells from old donors can proliferate but respond more slowly and to a lesser extent than cells from young donors. EGF added to the medium stimulates cells from old donors to enter the cell cycle faster, increases the relative number of actively cycling cells, and increases the number of cells exhibiting mitotic figures. The resultant hypothesis is that it is possible to stimulate a significant proliferative response in corneal endothelial cells from old individuals. Administration of an optimal combination of stimulatory growth factors is required under conditions in which cells have been transiently released from contact inhibition.
Selective Noncovalent Adsorption of Protein to Bifunctional Metallic Nanowire Surfaces (2003)

Metallic nanowires composed of nickel and gold as well as bimetallic nickel−gold nanowires were fabricated via templated electrodeposition in nanoporous alumina membranes. Gold surfaces were functionalized with alkanethiols with terminal hexa(ethylene glycol) groups (EG6), while nickel surfaces were functionalized with palmitic acid, a 16-carbon fatty acid. When exposed to a fluorescently tagged protein, hydrophobic nickel wires exhibited bright fluorescence while EG6-terminated gold wires did not, indicating that the protein did not adhere to the EG6-functionalized nanowires. Nickel−gold nanowires presenting distinct segments of alkyl and EG6 surfaces were also exposed to the fluorescent protein. Intense fluorescence was only observed on the nickel segment of these wires, demonstrating that proteins selectively adsorbed to one portion of these multicomponent nanostructures.
Perspectives on oblique angle deposition of thin films: From fundamentals to devices (2015)

The oblique angle configuration has emerged as an invaluable tool for the deposition of nanostructured thin films. This review develops an up to date description of its principles, including the atomistic mechanisms governing film growth and nanostructuration possibilities, as well as a comprehensive description of the applications benefiting from its incorporation in actual devices. In contrast with other reviews on the subject, the electron beam assisted evaporation technique is analyzed along with other methods operating at oblique angles, including, among others, magnetron sputtering and pulsed laser or ion beam-assisted deposition techniques. To account for the existing differences between deposition in vacuum or in the presence of a plasma, mechanistic simulations are critically revised, discussing well-established paradigms such as the tangent or cosine rules, and proposing new models that explain the growth of tilted porous nanostructures. In the second part, we present an extensive description of applications wherein oblique-angle-deposited thin films are of relevance. From there, we proceed by considering the requirements of a large number of functional devices in which these films are currently being utilized (e.g., solar cells, Li batteries, electrochromic glasses, biomaterials, sensors, etc.), and subsequently describe how and why these nanostructured materials meet with these needs.
Association between elevated plasma total homocysteine and increased common carotid artery wall thickness (1998)

Homocysteine is increasingly recognized as a risk factor for atherothrombotic arterial diseases. We investigated the relation between plasma concentrations of total homocysteine (tHcy) and common carotid artery intima-media wall thickness, measured by B-mode ultrasonography, in 513 asymptomatic men and women from eastern Finland aged 45-69 years. The subjects were examined in 1994-95 at the baseline of the Antioxidant Supplementation in Atherosclerosis Prevention (ASAP) study, a randomized double-blind placebo-controlled two by two factorial trial on the effect of vitamin E and C supplementation in the prevention of atherosclerotic progression. The subjects were assigned into two categories according to the plasma tHcy concentration; concentration over 11.5 micromol/L (highest quartile) or concentration below 11.5 micromol/L. In this study population the mean plasma tHcy concentration was 10.0 micromol/L, and the prevalence of plasma tHcy concentration exceeding 11.5 micromol/L was 33% in men and 18% in women. The adjusted mean intima-media thickness of the right and left common carotid arteries was 1.12 mm in men with elevated plasma tHcy concentration and 1.02 mm in men with a plasma tHcy concentration below 11.5 micromol/L (P = 0.029). In women there was no significant difference. We conclude that elevated plasma tHcy concentrations are associated with early atherosclerosis, as manifested by increased common carotid artery intima-media wall thickness, in middle-aged eastern Finnish men.
ZAP-70 Enhances BCR Signaling and Migration of Malignant B Lymphocytes towards CCL21 Via Induction of CCR7 Expression (2010)

Abstract Abstract 3591 ZAP-70 (ξ-associated protein) is a protein tyrosine kinase of the Syk/ZAP family that plays a crucial role in cellular activation in T and NK cells. High expression of ZAP-70 protein in malignant cells from Chronic Lymphocytic Leukemia (CLL) correlates with adverse clinical prognostic features, such as unmutated IgHV genes, short time to progression, and short survival. Moreover, ZAP-70 protein has been related to aggressive features of the CLL cells, such as enhanced B-cell receptor (BCR) signaling and higher migration capacity. To further investigate into the mechanisms by which ZAP-70 protein influences the clinical outcome of patients with CLL, we analyzed the functional consequences of ZAP-70 ectopic expression in malignant B-cells. For this, Ramos and Raji (Burkitt) B-cell lines were stably transfected with a ZAP-70 expressing vector (pEGFP-N2ZAP-70) and the effects in BCR signaling and migration were studied. BCR-expressing B-cells (Ramos) where stimulated with F(ab’)2 anti-IgM or F(ab’)2 anti-IgD. IgM but not IgD stimulation induced ZAP-70 activation and mobilization to the membrane, which, in turn, enhanced signaling through phosphorilation of AKT and ERK1-2 kinases. The presence of ZAP-70 also inhibited IgM and CD79b internalization after BCR stimulation, thus allowing for a longer and sustained stimulation. In Raji B-cells, which did not express BCR, ZAP-70 was constitutively phosphorilated, which induced a higher level of ERK1-2 phosphorilation only, suggesting that AKT phosphorilation in Ramos expressing ZAP-70 after IgM stimulation was depending on additional factors. BCR stimulation in Ramos B cells changed the expression pattern of several cytokine receptors such as CXCR4 and CXCR3 (downregulated) and CCR7 (upregulated). Interestingly, ZAP-70 activation was involved in the induction of CCR7 expression, which was significantly upregulated upon BCR stimulation in ZAP-70 expressing B-cells. This upregulation was also confirmed in Raji B cells upon ZAP-70 expression and activation. The relationship between ZAP-70 and CCR7 was also seen in neoplastic B-lymphocytes from patients with CLL where there was a linear correlation between the expressions of the two molecules. The increased CCR7 expression in ZAP-70 expressing B-cell lines translated into an enhanced signaling though CCR7 upon CCL21 addition and an enhanced migration towards a CCL21-containing medium in vitro. In conclusion, ZAP-70 ectopic expression leaded to an enhanced signaling through the BCR after IgM stimulation while it did not participate in the signaling through IgD. Moreover, ZAP-70 expression induced the upregulation of the chemokine receptor CCR7, thus giving the cells the ability to better respond and migrate towards CCL21. These results give further insight into the functional role of ZAP-70 protein in the aggressiveness of CLL cases with an increased expression of ZAP-70. Disclosures: No relevant conflicts of interest to declare.
대학생의 사이코그래픽 특성에 따른 관광지 선호유형 (2004)

This study puts its focus on understanding and analyzing tourist motivations, tourist types and finding preferences of tourist destinations from a...
A novel MLSD receiver architecture for nonlinear channels (2014)

A new architecture for maximum likelihood sequence detection (MLSD) in nonlinear dispersive channels (NLCs) is presented, and its robustness to inaccurate channel knowledge is analyzed. This architecture is developed by considering a novel orthogonal representation of the NLC, which is exploited to develop a front-end capable of obtaining uncorrelated symbol rate samples, representing a sufficient statistic for information decoding. This front-end is a special form of space-time whitened matched filter (ST-WMF), and the MLSD obtained by using this front-end (ST-WMF-MLSD) requires simple branch metrics due to the signal whitening. The ST-WMF also allows for space-time&nbsp; compression of the equivalent channel, which is exploited for further complexity reduction of the ST-WMF-MLSD. Simulation results show the good trade-off in performance and complexity obtained with the ST-WMF-MLSD, even in the presence of inaccurate channel knowledge.
High-Speed Optical Modulation Techniques (1985)

In this paper, transmitter and receiver components for microwave fiber optic links are reviewed. Present link signal to noise limitations imposed by the performance of these components are analyzed, and promising trends in component development are discussed.
The dentinogenic effect of mineral trioxide aggregate (MTA) in short‐term capping experiments (2002)

The present experiments indicate that MTA is an effective pulp-capping material, able to stimulate reparative dentine formation by the stereotypic defensive mechanism of early pulpal wound healing.
Fabrication and Characterization of Patterned Single-Crystal Silicon Nanolines (2007)

This letter demonstrates a method for fabricating single-crystal Si nanolines, with rectangular cross sections and nearly atomically flat sidewalls. The high quality of these nanolines leads to superb mechanical properties, with the strain to fracture measured by nanoindentation tests exceeding 8.5% for lines of 74 nm width. A large displacement burst before fracture was observed, which is attributed to a buckling mechanism. Numerical simulations show that the critical load for buckling depends on the friction at the contact surface.
Carbodihydrazones and oxalyl dihydrazones (1968)

Carbodihydrazones and oxalyl dihydrazones have been prepared using the reaction between aldehydes and carbohydrazide and oxalyl dihydrazide, respectively. The structures of these new series of compounds have been established by comparing their infrared absorption spectra with those of related compounds.The potential scope of the synthetic method has been indicated by the preparation of analogous derivatives from two aromatic aldehydes and from two simple ketones.
Rabies in Thailand (1998)

The prevalence of canine and human rabies in Thailand has decreased significantly during the last decade. This has been associated with an increasing number of human post-exposure treatments. Educational efforts, mass vaccination of dogs and cats and the use of safe and effective vaccines have all made an impact. The proportion of fluorescent antibody positive dogs, among those examined for rabies averaged 54% indicating that rabies is still a major public health threat. Canine rabies vaccination is not usually performed in animals < 3 months old. However, this study revealed that 14% of rabid dogs were < 3 months old and 42% were < or = 6 months old. This is the age group most likely to interact with humans and other dogs. Our study also supports the World Health Organization's recommendation that observing suspected rabid dogs for 10 days is an adequate and safe practice.
Floc size distribution and bacterial activities in membrane separation activated sludge processes for small-scale wastewater treatment/reclamation (1997)

Activated sludges taken from full-scale membrane separation processes, building wastewater reuse system (400m3/d), and two nightsoil treatment plants (50m3/d) as well as laboratory scale membrane separation bioreactor (0.062m3/d) were analyzed to characterize membrane separation activated sludge processes (MSAS). They were also compared with conventional activated sludges(CAS) taken from municipal wastewater treatment plants. Specific nitrification activity in MSAS processes averaged at 2.28gNH4-N/kgMLSS.h were higher than that in CAS processes averaged at 0.96gNH4-N/kgMLSS.h. The denitrification activity in both processes were in the range of 0.62-3.2gNO3-N/kgMLSS.h without organic addition and in the range of 4.25-6.4gNO3-N/kgMLSS.h with organic addition. The organic removal activity in nightsoil treatment process averaged at 123gCOD/kgMLSS.h which was significantly higher than others. Floc size distributions were measured by particle sedimentation technique and image analysis technique. Flocs in MSAS processes changed their sizes with MLSS concentration changes and were concentrated at small sizes at low MLSS concentration, mostly less than 60 μm. On the contrary, floc sizes in CAS processes have not much changed with MLSS concentration changes and they were distributed in large range. In addition, the effects of floc size on specific nitrification rate, denitrification rate with and without organic carbon addition were investigated. Specific nitrification rate was decreased as floc size increased. However, little effect of floc size on denitrification activity was observed.
CDK11 Complexes Promote Pre-mRNA Splicing (2003)

The PITSLRE protein kinases, hereafter referred to as CDK11 because of their association with the cyclin L regulatory partner, belong to large molecular weight protein complexes that contain RNA polymerase II. These CDK11(p110) complexes have been reported to influence transcription as well as interact with the general pre-mRNA-splicing factor RNPS1. Some of these complexes may also play a role in pre-mRNA splicing. Using a two-hybrid interactive screen, the splicing protein 9G8 was identified as an in vivo partner for CDK11(p110). The identification of several splicing-related factors as CDK11(p110) interactors along with the close relationship between transcription and splicing indicated that CDK11(p110) might influence splicing activity directly. Immunodepletion of CDK11(p110) from splicing extracts greatly reduced the appearance of spliced products using an in vitro assay system. Moreover, the re-addition of these CDK11(p110) immune complexes to the CDK11(p110)-immunodepleted splicing reactions completely restored splicing activity. Similarly, the addition of purified CDK11(p110) amino-terminal domain protein was sufficient to inhibit the splicing reaction. Finally, 9G8 is a phosphoprotein in vivo and is a substrate for CDK11(p110) phosphorylation in vitro. These data are among the first demonstrations showing that a CDK activity is functionally coupled to the regulation of pre-mRNA-splicing events and further support the hypothesis that CDK11(p110) is in a signaling pathway that may help to coordinate transcription and RNA-processing events.
Dangerous currents in the surf (1949)

Surf bathing leads to the drowning of thousands of people each summer. “Caught in the undertow” is the usual explanation for these disasters and yet there may be no such thing as undertow. In dictionaries it is defined as “the current beneath the surface which sets seaward when waves are breaking on the shore.” Many geological textbooks contain brief discussions of undertow, ascribing it to the seaward return of landward driven surface water.
Die Formatvorlage (1994)

Wenn Sie z. B. mit MS Word arbeiten, dann kennen Sie die Möglichkeit, sich individuelle Formatierungen von Text, Überschriften, Fußnoten usw. zurechtzulegen. Dies erspart das wiederholte Formatieren einzelner, gleichartiger Textpassagen.
Recent Developments in Rocket Nozzle Configurations (1961)

T THRUST of a rocket engine is primarily dependent upon the momentum imparted to the products of combustion by discharging them through an exhaust nozzle. During their passage through the nozzle, the exhaust gases are continuously accelerated from low subsonic velocities to high supersonic velocities at the nozzle exit. For the purpose of understanding the flow process, the nozzle can be divided into three portions: the convergent subsonic section, the throat section, and the divergent supersonic section. This division is appropriate because of the different effects each part has in determining the thrust developed by the rocket, and because different methods of analysis have to be used in determining the flow field in these three regions. The mass flow rate through a rocket nozzle is determined by the area of the throat section and operating conditions of the combustion chamber. Design changes in the configuration of the convergent portion of the nozzle would influence the mass flow of the exhaust gases and also, to some extent, the combustion efficiency achieved in the chamber. The sonic velocity attained by the exhaust gases is fixed by the combustion chamber conditions and can be further increased by the expansion occurring in the divergent supersonic portion of the nozzle. This additional velocity, leading to thrust increment, is dependent only on the configuration of the diverging nozzle walls and the exit area. In recent years the design of the divergent supersonic portion of the rocket nozzle has received considerable attention. The scope of this review will be limited to this portion of the nozzle and improvement thereof. Analysis of rocket nozzle flows in any real case should include radiative heat loss, chemical reactions due to incomplete combustion, and chemical properties of the exhaust gases. However, gross performance comparisons can be made by assuming that the exhaust gases expand adiabatically and behave like an ideal gas with a constant ratio of specific heat capacities. The appropriate choice of nozzle configuration for a specific rocket engine may, to a great extent, depend on the fabrication methods of nozzle walls, cooling requirements, allowable limits on dimensions, influence of nozzle weight on overall rocket performance, etc. Detailed examination of all these features involves various special engineering fields aside from the knowledge of supersonic expansion process of the
Animation in a peripheral display (2007)

Peripheral displays provide secondary awareness of news and information to people. When such displays are static, the amount of information that can be presented is limited and the display may become boring or routine over time. Adding animation to peripheral displays can allow them to show more information and can potentially enhance visual interest and appeal, but it may also make the display very distracting. Is it possible to employ animation for visual benefit without increasing distraction? We have created a peripheral display system called BlueGoo that visualizes R.S.S. news feeds as animated photographic collages. We present an empirical study in which participants did not find the system to be distracting, and many found it to be appealing. The study also explored how different display sizes and positions affect information conveyance and distraction. Animations on an angled second monitor appeared to be more distracting than three other configurations.
A rapid method for fluoride analysis of treated wood (2003)

A method for analyzing fluoride in wood by extraction in place of ashing was evaluated. Douglas-fir heartwood and ponderosa pine sapwood treated with various levels of fluoride were used for the analysis. Although the method tended to underestimate fluoride levels slightly, the time required for the assay was shorter than the current standard method, thus allowing the analysis of a greater number of samples.
Dual chirped optical pulses from a phase-modulated laser (2007)

Dual chirped optical pulse is reported by use of a phase-modulated laser. When a number of sidebands around the carrier frequency in a phase-modulated laser spectrum are spectrally carved, short optical pulse trains with dual chirp states are obtained. It is discussed that the pulse duration and the linear frequency chirp parameter can be found in closed form expressions given as a function of the modulation index and the modulation frequency when the phase modulation function of the dual-chirped pulse trains does not deviate severely from the modulation function applied to the phase modulator by spectral filtering effects. In experiments, optical pulse trains at 5 GHz repetition rate were generated with the pulse duration of 29.4 ps when frequency-modulated fiber laser signals with a 2.5 GHz repetition rate passed through a Gaussian-shaped optical band-pass filter (OBF) with a full width at half maximum (FWHM) 0.3 nm and a transmission peak at the carrier frequency of the frequency-modulated laser spectrum. Measured pulse duration was in good agreement with the analytical prediction value 28.2 ps and the numerical result 27.7 ps.
beta Blockade after myocardial infarction: systematic review and meta regression analysis (1999)

beta Blockers are effective in long term secondary prevention after myocardial infarction, but they are underused in such cases and lead to avoidable mortality and morbidity.
Prediction of active sites of enzymes by maximum relevance minimum redundancy (mRMR) feature selection (2012)

Identification of catalytic residues plays a key role in understanding how enzymes work. Although numerous computational methods have been developed to predict catalytic residues and active sites, the prediction accuracy remains relatively low with high false positives. In this work, we developed a novel predictor based on the Random Forest algorithm (RF) aided by the maximum relevance minimum redundancy (mRMR) method and incremental feature selection (IFS). We incorporated features of physicochemical/biochemical properties, sequence conservation, residual disorder, secondary structure and solvent accessibility to predict active sites of enzymes and achieved an overall accuracy of 0.885687 and MCC of 0.689226 on an independent test dataset. Feature analysis showed that every category of the features except disorder contributed to the identification of active sites. It was also shown via the site-specific feature analysis that the features derived from the active site itself contributed most to the active site determination. Our prediction method may become a useful tool for identifying the active sites and the key features identified by the paper may provide valuable insights into the mechanism of catalysis.
Cobalt-60: the heart of gamma-radiation sterilization. (1994)

Currently, more than three million cubic metres of single-use medical devices are sterilized by gamma radiation each year. The wide use of this method of sterilization is a result of the lethal effects of ionizing radiation on microbial populations and the penetrative powers of cobalt-60. This article describes how cobalt-60 is produced and used in gamma-radiation sterilization, and how it is disposed of once it has reached the end of its useful life. Cobalt-60 sources typically have a life of 20 years, at which point they are returned to the suppliers for re-encapsulation, reprocessing, recycling, or disposal.