gebhart/patrepeval huggingface dataset:
Analysis by gel electrophoresis, Western blot, and peptide mapping of protein A heterogeneity in Staphylococcus aureus strains (1987)

To evaluate potential differences in protein A among Staphylococcus aureus strains, lysostaphin-solubilized cell wall proteins from 12 serologically distinct strains were analyzed by 7.5% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Seven presumptive protein A variant identified in the 45- to 57-kilodalton range were then studied for qualitative binding affinities to nonimmune mouse and rabbit immunoglobulin G (IgG) by enzyme-linked immunoelectrotransfer blot. Essentially, all presumptive protein A variants demonstrated binding to both nonimmune rabbit and mouse IgG and had differential binding to mouse monoclonal IgG1 at pH 8.2 than at 5.5. Because of Fc-binding properties and molecular weight similarity to the well-characterized Cowan I protein A, these proteins appeared to represent protein A variants. Amino sugar analysis (less than 1%) by reverse-phase high-pressure liquid chromatography suggested that the apparent molecular weight differences in protein A were not due to associated mucopeptides. Further differences in protein A variants were studied by peptide mapping. Each of the seven protein A variants, distinguishable on SDS-PAGE, also produced distinct peptide cleavage patterns. In addition, two protein A variants indistinguishable on SDS-PAGE could be further subdivided by peptide mapping. These results suggest that SDS-PAGE analysis of protein A, particularly in conjunction with peptide mapping, may be useful in distinguishing distinct strains of S. aureus. Different protein A variants may also have unique functional or immunologic capabilities.
Dynamic time warping for off-line recognition of a small gesture vocabulary (2002)

We focus on the visual sensory information to recognize human activity in form of hand-arm movements from a small, predefined vocabulary. We accomplish this task by means of a matching technique by determining the distance between the unknown input and a set of previously defined templates. A dynamic time warping algorithm is used to perform the time alignment and normalization by computing a temporal transformation allowing the two signals to be matched. The system is trained with finite video sequences of single gesture performances whose start and end-point are accurately known. Preliminary experiments are accomplished off-line and result in a recognition accuracy of up to 92%.
Mode of Action of Tacrolimus (FK506): Molecular and Cellular Mechanisms (1995)

Tacrolimus, formerly known as FK506, is a macrolide antibiotic with immunosuppressive properties. Although structurally unrelated to cyclosporin A (CsA), its mode of action is similar. It exerts its effects principally through impairment of gene expression in target cells. Tacrolimus bonds to an immunophilin, FK506 binding protein (FKBP). This complex inhibits calcineurin phosphatase. The drug inhibits calcium-dependent events, such as interleukin-2 gene transcription, nitric oxide synthase activation, cell degranulation, and apoptosis. Tacrolimus also potentiates the actions of glucocorticoids and progesterone by binding to FKBPs contained within the hormone receptor complex, preventing degradation. The agent may enhance expression of the transforming growth factor beta-1 gene in a fashion analogous to that demonstrated for CsA. T cell proliferation in response to ligation of the T cell receptor is inhibited by tacrolimus. Type 1 T helper cells appear to be preferentially suppressed compared with type 2 T helper cells. T cell-mediated cytotoxicity is impaired. B cell growth and antibody production are affected indirectly by the suppression of T cell-derived growth factors necessary for these functions. Antigen presentation appears to be spared. The molecular events affected by tacrolimus continue to be discovered.
D-Sempre: Learning Deep Semantic-Preserving Embeddings for User interests-Social Contents Modeling (2018)

Exponential growth of social media consumption demands effective user interests-social contents modeling for more personalized recommendation and social media summarization. However, due to the heterogeneous nature of social contents, traditional approaches lack the ability of capturing the hidden semantic correlations across these multi-modal data, which leads to semantic gaps between social content understanding and user interests. To effectively bridge the semantic gaps, we propose a novel deep learning framework for user interests-social contents modeling. We first mine and parse data, i.e. textual content, visual content, social context and social relation, from heterogeneous social media feeds. Then, we design a two-branch network to map the social contents and users into a same latent space. Particularly, the network is trained by a large margin objective that combines a cross-instance distance constraint with a within-instance semantic-preserving constraint in an end-to- end manner. At last, a Deep Semantic-Preserving Embedding (D-Sempre) is learned, and the ranking results can be given by calculating distances between social contents and users. To demonstrate the effectiveness of D-Sempre in user interests-social contents modeling, we construct a Twitter dataset and conduct extensive experiments on it. As a result, D-Sempre effectively integrates the multi-modal data from heterogeneous social media feeds and captures the hidden semantic correlations between users' interests and social contents.
Human Immunodeficiency Virus Antigenemia (1987)

The article by Kessler et al 1 in this issue ofThe Journaldescribes the acute illness associated with human immunodeficiency virus (HIV) infection in four individuals. Of interest was the detection of HIV antigenemia during the course of this self-limited illness before enzyme-linked immunosorbent and Western blot assays for antibody to HIV became positive. The presence of circulating antigen thus helped to establish the diagnosis. Other investigators 2 have reported that HIV antigenemia precedes seroconversion in an unknown proportion of acutely infected individuals. The evidence available to date suggests that the circulating antigen contains the core protein of the HIV (p24) and possibly other epitopes of the virus, and that when antibody to the p24 component of the virus increases to measurable concentrations in serum or plasma, the antigen can no longer be detected. 3 In some individuals, months to years after infection, anti-p24 concentrations fall in association with
<b>Characterization of an </b><i><b>AGAMOUS</b></i><b> homologue from the conifer black spruce (</b><i><b>Picea mariana</b></i><b>) that produces floral homeotic conversions when expressed in </b><i><b>Arabidopsis</b></i> (1998)

Advances in elucidating the molecular processes controlling flower initiation and development have provided unique opportunities to investigate the developmental genetics of non-flowering plants. In addition to providing insights into the evolutionary aspects of seed plants, identification of genes regulating reproductive organ development in gymnosperms could help determine the level of homology with current models of flower induction and floral organ identity. Based upon this, we have searched for putative developmental regulators in conifers with amino acid sequence homology to MADS-box genes. PCR cloning using degenerate primers targeted to the MADS-box domain revealed the presence of over 27 MADS-box genes within black spruce (Picea mariana), including several with extensive homology to either AP1 or AGAMOUS, both known to regulate flower development in Arabidopsis. This indicates that like angiosperms, conifers contain a large and diverse MADS-box gene family that probably includes regulators of reproductive organ development. Confirmation of this was provided by the characterization of an AGAMOUS-like cDNA clone called SAG1, whose conservation of intron position and tissue-specific expression within reproductive organs indicate that it is a homologue of AGAMOUS. Functional homology with AGAMOUS was demonstrated by the ability of SAG1 to produce homeotic conversions of sepals to carpels and petals to stamens when ectopically expressed in transgenic Arabidopsis. This suggests that some of the genetic pathways controlling flower and cone development are homologous, and antedate the 300-million-year-old divergence of angiosperms and gymnosperms.
Tinnitus and Other Auditory Problems – Occupational Noise Exposure below Risk Limits May Cause Inner Ear Dysfunction (2014)

The aim of the investigation was to study if dysfunctions associated to the cochlea or its regulatory system can be found, and possibly explain hearing problems in subjects with normal or near-normal audiograms. The design was a prospective study of subjects recruited from the general population. The included subjects were persons with auditory problems who had normal, or near-normal, pure tone hearing thresholds, who could be included in one of three subgroups: teachers, Education; people working with music, Music; and people with moderate or negligible noise exposure, Other. A fourth group included people with poorer pure tone hearing thresholds and a history of severe occupational noise, Industry. Ntotal = 193. The following hearing tests were used: - pure tone audiometry with Békésy technique, - transient evoked otoacoustic emissions and distortion product otoacoustic emissions, without and with contralateral noise; - psychoacoustical modulation transfer function, - forward masking, - speech recognition in noise, - tinnitus matching. A questionnaire about occupations, noise exposure, stress/anxiety, muscular problems, medication, and heredity, was addressed to the participants. Forward masking results were significantly worse for Education and Industry than for the other groups, possibly associated to the inner hair cell area. Forward masking results were significantly correlated to louder matched tinnitus. For many subjects speech recognition in noise, left ear, did not increase in a normal way when the listening level was increased. Subjects hypersensitive to loud sound had significantly better speech recognition in noise at the lower test level than subjects not hypersensitive. Self-reported stress/anxiety was similar for all groups. In conclusion, hearing dysfunctions were found in subjects with tinnitus and other auditory problems, combined with normal or near-normal pure tone thresholds. The teachers, mostly regarded as a group exposed to noise below risk levels, had dysfunctions almost identical to those of the more exposed Industry group.
MULTI-TON PRODUCTION OF FLUORINE FOR MANUFACTURE OF URANIUM HEXAFLUORIDE (1958)

The evolution and operation of full-scale fluorine production facilities are described. Flow diagrams, scaled drawings of process equipment, and photographs of systems and individual items of equipment are pre- -- -- -sented. (W.D.M.)
Herpes simplex virus gene expression in neurons: viral DNA synthesis is a critical regulatory event in the branch point between the lytic and latent pathways (1996)

Herpes simplex virus establishes a latent infection in peripheral neurons. We examined viral gene expression in rat peripheral neurons in vitro and determined that viral gene expression is attenuated and delayed in these neurons compared with that in Vero cells. In addition, using pharmacologic and genetic blocks to viral DNA synthesis, we found that viral alpha and beta gene expression was upregulated by viral DNA synthesis. Although maximal gene expression in neurons requires viral DNA synthetic activity, activation of viral gene expression was seen even in the presence of herpes simplex virus DNA polymerase inhibitors, but not in the absence of the origin-binding protein. Initiation of viral DNA synthesis is apparently a key regulatory event in the balance between the lytic and latent pathways in peripheral neurons.
Antifungal activity of the new agent latoconazole in two tinea models. (1991)

In vitro and in vivo antifungal activities of the new imidazole derivative latoconazole ((+-)-(E)-[4-(2-chlorophenyl)-1,3-dithiolan-2-ylidene]-1- imidazolylacetonitrile, NND-318; CAS 101530-10-3) were studied in comparison with three major topical agents, clotrimazole, bifonazole and tolnaftate. The in vitro activity of latoconazole against dermatophytes was much stronger than that of any reference agent tested. Both the recently developed tinea pedis model and the conventional tinea model in guinea pigs were employed for evaluation of topical usefulness of latoconazole. The 1% solution or cream preparation of latoconazole was highly effective in both of the two tinea models and its 5 or more doses achieved almost complete mycological cure. However, both tinea models, especially the former, were considerably resistant to the therapeutic treatment of all of the reference drugs. These results suggest that latoconazole is a promising topical antifungal agent, probably applicable to the treatment of tinea pedis as well as other types of dermatomycoses.
Sustainable Waste Tire Derived Carbon Material as a Potential Anode for Lithium-Ion Batteries (2018)

The rapidly growing automobile industry increases the accumulation of end-of-life tires each year throughout the world. Waste tires lead to increased environmental issues and lasting resource problems. Recycling hazardous wastes to produce value-added products is becoming essential for the sustainable progress of society. A patented sulfonation process followed by pyrolysis at 1100 °C in a nitrogen atmosphere was used to produce carbon material from these tires and utilized as an anode in lithium-ion batteries. The combustion of the volatiles released in waste tire pyrolysis produces lower fossil CO2 emissions per unit of energy (136.51 gCO2/kW·h) compared to other conventional fossil fuels such as coal or fuel–oil, usually used in power generation. The strategy used in this research may be applied to other rechargeable batteries, supercapacitors, catalysts, and other electrochemical devices. The Raman vibrational spectra observed on these carbons show a graphitic carbon with significant disorder structure. Further, structural studies reveal a unique disordered carbon nanostructure with a higher interlayer distance of 4.5 Å compared to 3.43 Å in the commercial graphite. The carbon material derived from tires was used as an anode in lithium-ion batteries exhibited a reversible capacity of 360 mAh/g at C/3. However, the reversible capacity increased to 432 mAh/g at C/10 when this carbon particle was coated with a thin layer of carbon. A novel strategy of prelithiation applied for improving the first cycle efficiency to 94% is also presented.
Ribavirin Therapy for Adenovirus Pneumonia in an AIDS Patient (1997)

We report the effectiveness of ribavirin in an AIDS patient with multinodular pneumonia due to adenovirus. A 38-year-old AIDS patient who experienced multiple opportunistic infections and whose CD4 lymphocyte count was 5/mm3 developed bilateral nodular lung opacities. Lung surgical biopsy yielded necrotizing pneumonitis with characteristic nuclear inclusions and positive immunocytology with adenovirus antibodies. Marked clinical and radiological improvement was obtained after intravenous then oral ribavirin. Ribavirin was discontinued after 40 d because of anemia. Relapse of pneumonia with respiratory distress led to death 8 mo later. This observation illustrates a rarely reported pulmonary opportunistic infection in AIDS and the potential value of ribavirin therapy for adenovirus pneumonia.
Printhead carriage transport high speed control system with robust compensator (2002)

A robust controller has been developed for print-head carriage transportation. The controller consists of a linear-quadratic Gaussian (LQG) controller and a robust compensator. Applying LQG control, a print-head carriage transport velocity of about 1.2 m/sec without vibrations was achieved. The acceleration and deceleration areas were within 30 mm. However, compensation with only LQG control has problems regarding deterioration in the control, resulting from environmental variations and aging. The proposed robust compensator prevented the deterioration in control results by cancelling the equivalent disturbance, which corresponds to the environmental variations and aging. Moreover, the optimal controller configuration was composed by using a discrete design for the robust compensator. According to the experimental results, the velocity response errors from the reference, in case of the robust controller, were within 5%, even if the motor temperature changed from 20/spl deg/C to about 60/spl deg/C. Employing the robust controller, a dot serial printer, whose printing speed is 495 characters per second in pica printing mode, has been developed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Novel Peptide Derived from Human Apolipoprotein E Is an Inhibitor of Tumor Growth and Ocular Angiogenesis (2011)

Angiogenesis is a hallmark of tumor development and metastasis and now a validated target for cancer treatment. We previously reported that a novel dimer peptide (apoEdp) derived from the receptor binding region of human apolipoprotein E (apoE) inhibits virus-induced angiogenesis. However, its role in tumor anti-angiogenesis is unknown. This study demonstrates that apoEdp has anti-angiogenic property in vivo through reduction of tumor growth in a mouse model and ocular angiogenesis in a rabbit eye model. Our in vitro studies show that apoEdp inhibits human umbilical vein endothelial cell proliferation, migration, invasion and capillary tube formation. We document that apoEdp inhibits vascular endothelial growth factor-induced Flk-1 activation as well as downstream signaling pathways that involve c-Src, Akt, eNOS, FAK, and ERK1/2. These in vitro data suggest potential sites of the apoE dipeptide inhibition that could occur in vivo.This is the first evidence that a synthetic dimer peptide mimicking human apoE has anti-angiogenesis functions and could be an anti-tumor drug candidate.
Image hashing resilient to geometric and filtering operations (2005)

Image hash functions provide compact representations of images, which is useful for search and authentication applications. In this work, we have identified a general three step framework and proposed a new image hashing scheme that achieves a better overall performance than the existing approaches under various kinds of image processing distortions. By exploiting the properties of discrete polar Fourier transform and incorporating cryptographic keys, the proposed image hash is resilient to geometric and filtering operations, and is secure against guessing and forgery attacks.
Transcription Factor PU.1 Is Necessary for Development of Thymic and Myeloid Progenitor-Derived Dendritic Cells (2000)

Dendritic cells (DCs) are a heterogeneous population of cells that are specialized for Ag processing and presentation. These cells are believed to derive from both myeloid- and lymphoid-committed precursors. Normal human PBMC-derived, human CD14+ cell (monocyte)-derived, and mouse hematopoietic progenitor-derived DCs were shown to express the hematopoietic cell-restricted, ets family transcription factor PU.1. These populations represent myeloid progenitor-derived DCs. Hematopoietic progenitor cells from PU.1 gene-disrupted (null) mice were unable to generate MHC class IIhigh, CD11c+ myeloid-derived DCs in vitro. Mouse thymic DCs are proposed to be derived from a committed lymphoid progenitor cell that can give rise to T cells as well as DCs. Previously, we showed that CD4 and CD8 T cells developed in PU.1 null mice in a delayed manner and in reduced number. We examined the thymus of 10- to 12-day-old PU.1 null mice and found no evidence of DEC-205+, MIDC-8+ DCs in this tissue. Our findings indicate that PU.1 regulates the development of both thymic and myeloid progenitor-derived populations of DCs, and expand its known role in hematopoietic development.
Blockchain and Smart Contracts for Insurance: Is the Technology Mature Enough? (2018)

Blockchain is receiving increasing attention from academy and industry, since it is considered a breakthrough technology that could bring huge benefits to many different sectors. In 2017, Gartner positioned blockchain close to the peak of inflated expectations, acknowledging the enthusiasm for this technology that is now largely discussed by media. In this scenario, the risk to adopt it in the wake of enthusiasm, without objectively judging its actual added value is rather high. Insurance is one the sectors that, among others, started to carefully investigate the possibilities of blockchain. For this specific sector, however, the hype cycle shows that the technology is still in the innovation trigger phase, meaning that the spectrum of possible applications has not been fully explored yet. Insurers, as with many other companies not necessarily active only in the financial sector, are currently requested to make a hard decision, that is, whether to adopt blockchain or not, and they will only know if they were right in 3–5 years. The objective of this paper is to support actors involved in this decision process by illustrating what a blockchain is, analyzing its advantages and disadvantages, as well as discussing several use cases taken from the insurance sector, which could easily be extended to other domains.
Immobilization of β‐galactosidase, albumin, and γ‐globulin on epoxy‐activated acrylic beads (1980)

A comparative study was conducted into the immobilization of beta-galactosidase, albumin, and gamma-globulin on an epoxy-activated polyacrylic matrix (oxirane C, Röhm-Pharma GmbH, Darmstadt). The kinetic parameters of the immobilized beta-galactosidase were investigated with three kinds of miniaturized analytical reactors; namely, stirred batch, continuous stirred-tank, and packed-bed reactors. The optimum binding conditions, saturation activity and Michaelis constant of immobilized beta-galactosidase are given, together with determinations of the binding capacity of the oxirane C matrix for the three proteins investigated. For beta-galactosidase a saturation activity of 1300 U/g oxirane C was reached. The maximum binding, achieved by experiment, was 140 mg/g with 0.69 yield for albumin, 120 mg/g with 0.61 yield for gamma-globulin, and 40 mg/g with 0.42 yield for beta-galactosidase. From these data the inner surface of the matrix as a function of the size of the bound proteins was estimated.
MIMO-OFDMA Opportunistic Beamforming with Partial Channel State Information (2006)

Over the past few years, a lot of interest has focused on Orthogonal Frequency Division Multiple Access (OFDMA) and Multiple Input Multiple Output (MIMO) systems thanks to the tremendous gain on system capacity they offer. Transmit beamforming is a low complexity technique that helps in achieving the full diversity afforded by the multiple antenna environment. MIMO-OFDMA systems using opportunistic beamforming are a promising solution to satisfy the growing demand in terms of data rate and Quality of Service (QoS). An important practical issue in MIMO-OFDMA systems is the feedback load. As a large number of carriers (e.g. 2048 for WiMax) is usually used in such systems, feeding back full Channel State Information at the transmitter (CSIT) for each carrier is prohibitive. In this paper, the problem of feedback reduction in MIMO-OFDMA opportunistic beamforming is addressed. We present different partial CSIT schemes that reduce significantly the feedback overload at little expense of system throughput.
Synthesis and Inclusion Properties of “Neutral” Water-Soluble Calixarenes (1990)

Abstract “Neutral” water-soluble calix[n]arenes, 1n (n=6 and 8) were synthesized for the first time. Below the critical micelle concentrations, they formed host–guest-type complexes with naphthalenesulfonate derivatives used as fluorescence probes. The binding sites showed the hydrophobicity comparable with those of cyclodextrins, and the cavities showed the shape selectivity for these guest molecules.