gebhart/patrepeval huggingface dataset:
Studies of the biogenic amine transporters. 10. Characterization of a novel cocaine binding site in brain membranes prepared from dopamine transporter knockout mice (2002)

Previous work suggested that the cocaine analog [(125)I]RTI-55 labels a novel binding site in rat brain membranes, which is not associated with the dopamine (DA), serotonin (5-HT), or norepinephrine (NE) transporters [Rothman et al. 1995 J Pharmacol Exp Ther 274:385-395]. Here, we tested whether this site is a product of the DA transporter (DAT) gene. We used a T-antigen knock-in at the DAT gene that results in an effective DAT knock-out (KO) confirmed by Southern blot, DAT immunohistochemistry, and [(125)I]RTI-55 ligand binding. Brain membranes were prepared from frozen whole brain minus caudate of wild-type (WT) B6/Sv129, +/+ and minus sign/minus sign (KO) mice. KO mice were used at approximately 23 days of age. Binding surface analysis of [(125)I]RTI-55 binding to membranes prepared from the brains of WT mice, with 100 nM citalopram to block binding to the 5-HT transporter (SERT), revealed two binding sites: the DAT and a second site, replicating previous studies conducted with rat brains. In the absence of the DAT (minus sign/minus sign mice), binding surface analysis demonstrated that [(125)I]RTI-55 labeled two sites: the NET and a second site called site "X." Structure-activity studies of site "X" demonstrated that high-affinity ligands for the DAT, NET, and SERT have low or negligible affinity for site "X." The relatively high density of site "X" in brain membranes and the fact that the K(i) values of cocaine and cocaethylene for site "X" are in the range achieved in the brain following cocaine administration suggests that site "X" could contribute to the pharmacological or toxicological effects of cocaine. Further progress in delineating the function of site "X" will depend on developing potent and selective agents for this site.
Magnetization transfer ratio evolution with demyelination and remyelination in multiple sclerosis lesions (2008)

We found significant changes in MTR consistent with demyelination and remyelination that followed different temporal evolutions and were ongoing in different lesion regions for at least 3 years after lesion formation.
A Novel 1.5 $^primeprime$ Quadruple Antenna for Tri-Band GPS Applications (2006)

A new global positioning system (GPS) antenna is proposed to cover the three GPS bands (L1, L2, and L5, namely 1575, 1227, and 1176 MHz) with the L5 band to be added after 2006. The developed antenna size is only $1.5^primeprimetimes 1.5^primeprime$ in aperture corresponding to $lambda/7timeslambda/7$ $(lambda=hboxfree hboxspace hboxwavelength)$ and $lambda/13 hboxthick$. Quadrature feeding is employed to ensure right-hand circular polarized (RHCP) radiation. The final miniature antenna exhibits a gain greater than 2 dBi, and to our knowledge this is the smallest such size for circular polarized (CP) operation covering all three bands. Detailed parametric simulations leading to the best design along with measurements for the constructed antenna are presented.
CO<sub>2</sub> Sorption Performance of Composite Polymer/Aminosilica Hollow Fiber Sorbents: An Experimental and Modeling Study (2015)

The dynamic CO2 sorption performance of polymer/silica supported polyethylenimine hollow fiber sorbents (CA-S-PEI), focusing on heat and mass transport effects, is investigated experimentally and computationally during sorption of CO2 from simulated, dry flue gases. The effect of the nonisothermality on the sorption performance is investigated by varying the module materials of construction. The heat effects are minimized by using a heat conductive module case with a diameter of 0.25 in., and, accordingly, the breakthrough capacities are increased by 30% over a similar module constructed from less conductive components, thereby improving fiber sorbents utilization efficiency. The sorption kinetics in CA-S-PEI hollow fiber sorbents are investigated in terms of flow rates, module packing fraction, module length, and silica particle size. A mathematical model developed previously is successfully utilized to predict various contributions to the overall mass transfer resistance. In fiber sorbents where the amine loading is high, such as those employed here, the sorption process is found to be controlled by intraparticle mass transfer resistances. Unlike fiber sorbents based on physisorbents, the external gas diffusion resistance has minimal effects on the breakthrough capacities, as evidenced with the negligible effects of the module packing fraction on the sorption capacities. Sorption capacities are found to increase with the fiber module length as a result of self-sharpening effects. The increase of particle size increases the mass transfer resistance of the fiber sorbents as illustrated by the more diffuse CO2 breakthrough fronts in fiber modules containing bigger silica particles. The capacities in fiber sorbents with the largest silica particles exhibit the lowest sorption capacity, as expected.
Computer Networking: Internet Protocols in Action (2005)

Preface. Acknowledgements. Section 1: Getting Started. 1.0 Introduction. 1.1 Examining a Quiet Network with Ethereal. 1.2 Protocol Layering. 1.3 Examining a Busy Network Using Filters. Section 2: Application Layer Protocols. 2.0 Introduction. 2.1 Under the Hood of HTTP. 2.2 HTTP Caching, Authorization and Cookies. 2.3 FTP-File Transfer Protocol. 2.4 Sending and Receiving Email with SMTP and POP. Section 3: Transport Layer Protocols. 3.0 Introduction. 3.1 Simple TCP Stream. 3.2 Retransmission in TCP. 3.3 Comparing TCP to UDP. 3.4 Competing TCP and UDP Streams. Section 4: Network Layer Protocols. 4.0 Introduction. 4.1 Joining the Internet: Introduction to IP and DHCP. 4.2 Ping and Traceroute. 4.3 Dynamic Routing with RIP. Section 5: Link Layer Protocols. 5.0 Introduction. 5.1 MAC Addresses. 5.2 Ethernet. 5.3 Wireless LANs. Section 6: Security. 6.0 Introduction. 6.1 Encryption. 6.2 IP Spoofing and TCP Session Stealing. 6.3 System Vulnerabilities. Index.
Resonant microwave cavity response of amorphous ribbons (1996)

In this study we report microwave experiments on the amorphous ribbon Fe4.6Co70.4Si15B10 using the usual setup for magnetic resonance experiments at 9.4 GHz to observe the cavity response. Several samples cut from the same tape were annealed, either in the presence or in the absence of a magnetic field. For each sample the magnetization and magnetoimpedance (MI) curves were obtained, with MI ratios between 1% and 40%. The magnetic resonance spectrum consists of the usual ferromagnetic line and an anomalous low field cavity response for H&lt;100 Oe. The shape of this low field signal depends on the thermal treatment and on the orientation of the external field. We believe that this signal is in part a ferromagnetic peak but it also carries information of the skin-depth changes with a sweeping magnetic field.
Protein Kinase C-dependent Regulation of NAG-1/Placental Bone Morphogenic Protein/MIC-1 Expression in LNCaP Prostate Carcinoma Cells (2005)

NAG-1 (nonsteroidal anti-inflammatory drug-activated gene), a member of the transforming growth factor beta superfamily, is involved in cellular processes such as inflammation, apoptosis/survival, and tumorigenesis and is regulated by p53, Sp1, and Egr-1. In the current study, the regulation of NAG-1 expression in LNCaP human prostate carcinoma cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) was examined. TPA treatment increased NAG-1 protein and mRNA levels in a time- and concentration-dependent manner as well as NF-kappa B binding/transcriptional activity in LNCaP cells. Pretreatment with protein kinase C (PKC) inhibitor blocked the TPA-induced increase in NAG-1 protein levels and NF-kappa B binding/transcriptional activity, whereas an inhibition of p38, JNK, MEK activity had no effect on TPA-induced NAG-1 levels and NF-kappa B transcriptional activity. Expression of constitutively active PKCs induced an increase in NF-kappa B transcriptional activity and NAG-1 protein levels in LNCaP cells. The expression of NF-kappa B p65 induced NAG-1 promoter activity, and chromatin immunoprecipitation assay for p65 showed that NF-kappa B binds the NAG-1 promoter in LNCaP cells. Inhibition of TPA-induced NAG-1 expression by NAG-1 short interfering RNA blocked TPA-induced apoptosis in LNCaP cells, suggesting induction of NAG-1 negatively affects LNCaP cell survival. These results demonstrate that NAG-1 expression is up-regulated by TPA in LNCaP cells through a PKC-dependent pathway involving the activation of NF-kappa B.
Comparative biodistribution of iodinated amino acids in rats: selection of the optimal analog for oncologic imaging outside the brain. (2003)

(123)I-2-IT and (123)I-2-IPhe are promising tracers for oncologic imaging outside the brain. (123)I-2-IT has the advantage of an established kit for radiosynthesis.
Regulated expression of the <i>Leishmania major</i> surface virulence factor lipophosphoglycan using conditionally destabilized fusion proteins (2009)

Surface glycoconjugates play important roles in the infectious cycle of Leishmania major, including the abundant lipophosphoglycan (LPG) implicated in parasite survival in the sand fly vector and the initial stages of establishment in the mammalian host macrophage. We describe a system for inducible expression of LPG, applying a novel protein-based system that allows controlled degradation of a key LPG biosynthetic enzyme, UDP-galactopyranose mutase (UGM). This methodology relies on a mutated FK506-binding protein (FKBP) destabilizing domain (dd) fused to the protein of interest; in the absence of rapamycin analogs, such as Shld1, the dd domain is destabilized, leading to proteasomal degradation, whereas drug treatment confers stabilization. Tests in L. major using dd fusions to a panel of reporters and cellular proteins confirmed its functionality, with a high degree of regulation and low background, and we established the kinetics of protein activation and/or loss. Two inexpensive and widely available ligands, FK506 and rapamycin, functioned similarly to Shld1, without effect on Leishmania growth or differentiation. We generated parasites lacking UGM through deletion of the GLF gene and substitution with a ddGLF fusion construct, either as chromosomal knockins or through episomal complementation; these showed little or no LPG expression in the absence of inducer, whereas in its presence, high levels of LPG were attained rapidly. Complement lysis tests confirmed the correct integrity of the Leishmania LPG coat. These data suggest that the dd approach has great promise in the study of LPG and other pathways relevant to parasite survival and virulence.
PEDOT: PSS as a Functional Binder for Cathodes in Lithium Ion Batteries (2015)

The application areas of rechargeable Li-ion batteries continue to grow, hence improvement in their energy density, rate capability and cycle life is necessary. A typical cathode contains usually redox active transition metal oxides as active materials, conductive additives to ensure electronic conductivity and binder supporting matrix. In this work we report the behavior and properties of carbon black free LiFePO4 composite electrode, where poly (3,4-ethylene dioxythiophene): poly (styrene sulfonate) (PEDOT:PSS) is accomplishing a dual role of binder and conducting additive. The effect of the polymer amount on the morphometric properties of the electrodes was studied using SEM, mercury porosimetry and high resolution X-ray computed tomography. The electrochemical performance and the cycling stability of the composite electrodes were compared to the behavior of conventional cathodes with carbon additives and PVDF binder. With increasing PEDOT:PSS content a decrease in the overvoltage and correspondingly an improvement in the rate capability is observed. Composite cathodes containing 8% PEDOT:PSS show comparable electrode capacity and better cyclic stability than conventional composite cathode.
Topology Discovery in Large Ethernet Mesh Networks (2006)

Automatic discovery of physical topology plays a crucial role in enhancing the manageability of modem large Ethernet mesh networks. Despite the importance of the problem, earlier research and commercial network management tools have typically concentrated on either discovering active topology, or proprietary solutions targeting specific product families. Recent works [1]-[3] have demonstrated that physical topology can be determined using standard SNMP MIB, but these algorithms depend on Filtering Database and rely on the so-called spanning tree protocol (IEEE 802.1d) in order to break cycles, thereby avoiding the possibility of infinitely circulating packets and deadlocks. A previous work [1] requires that Filtering Database entries are completed; however it is a very critical assumption in a realistic Ethernet mesh network. In this paper, we have proposed a new topology discovery algorithm which works without the complete knowledge of Filtering Database. Our algorithm can discover complete physical topology including inactive interfaces eliminated by the spanning tree protocol in LEMNs. The effectiveness of the algorithm is demonstrated by an implementation.
Pathophysiology and management of septic shock. (1980)

Septic shock is an acute medical and surgical emergency with a devastating mortality rate. It may occur in both debilitated, immunosuppressed patients and young, healthy patients undergoing genitourinary instrumentation. This paper reviews the intricate pathophysiology of septic shock and presents a comprehensive plan for the management of this life-threatening illness.
Study of waveguide crosstalk in silicon photonics integrated circuits (2013)

Silicon photonics is going trough a terrific expansion driven by several applications, from chip wiring to integrated sensors and telecommunications. Some applications, e.g. intra and inter chip connections and sensing, require long parallel waveguides for wiring or for connecting grating couplers (GCs) to devices situated in sensing micro-channels. In well packed photonics chips there are often long wiring waveguides parallel for several mm, so loss can be caused by light coupled back and forth between them (cross-talk), by scattering, wall roughness, mode mismatch, etc. This work aims to investigate cross-talk for long parallel waveguides, and to propose methods to reduce cross-talk loss when high integration density is required. We have designed and fabricated about 200 testing structures exploiting e-beam on silicon on insulator (SOI) chip, in order to test several parameters and to find out dominant loss mechanisms. All devices have been tested and measured using an automatic optical bench, in the wavelength range between 1500-1600 nm. Achieved results are promising, since they allow for comparing cross-talk for short as well as long interaction lengths (up to 5 mm), different waveguide width pairs, several separation distances, and for TE and TM polarization. For smaller gaps, having not symmetric pair of waveguides is very beneficial, since it results in a lower power coupling, e.g. about 20/14 dB of crosstalk reduction for TE/TM waveguides after 5 mm of propagation and gap of 0.5 μm. This can be very useful for the design of integrated photonics chips requiring high-density packaging of devices and waveguides.
Three‐Dimensional Writing of Conducting Polymer Nanowire Arrays by Meniscus‐Guided Polymerization (2011)

Accurate and versatile three-dimensional writing of individually controlled conducting polymer nanodevices forming dense arrays is demonstrated by guiding a monomer meniscus in pulling a micropipette during oxidative polymerization. We specifically demonstrate well-defined dense arrays of various freestanding nanocomponents with controlled radius down to ∼50 nm: straight wires, nanowires with variable radius, branches, and bridges.
How Much DNA Is Lost? Measuring DNA Loss of Short-Tandem-Repeat Length Fragments Targeted by the PowerPlex 16® System Using the Qiagen MinElute Purification Kit (2014)

The success in recovering genetic profiles from aged and degraded biological samples is diminished by fundamental aspects of DNA extraction, as well as its long-term preservation, that are not well understood. While numerous studies have been conducted to determine whether one extraction method was superior to others, nearly all of them were initiated with no knowledge of the actual starting DNA quantity in the samples prior to extraction, so they ultimately compared the outcome of all methods relative to the best. Using quantitative PCR to estimate the copy count of synthetic standards before (i.e., "copies in") and after (i.e., "copies out") purification by the Qiagen MinElute PCR Purification Kit, we documented DNA loss within a pool of 16 different-sized fragments ranging from 106 to 409 bp in length, corresponding to those targeted by the PowerPlex 16 System (Promega, Madison, WI). Across all standards from 10(4) to 10(7) copies/μL, loss averaged between 21.75% and 60.56% (mean, 39.03%), which is not congruent with Qiagen's claim that 80% of 70 bp to 4 kb fragments are retained using this product (i.e., 20% loss). Our study also found no clear relationship either between DNA strand length and retention or between starting copy number and retention. This suggests that there is no molecule bias across the MinElute column membrane and highlights the need for manufacturers to clearly and accurately describe on what their claims are based, and should also encourage researchers to document DNA retention efficiencies of their own methods and protocols. Understanding how and where to reduce loss of molecules during extraction and purification will serve to generate clearer and more accurate data, which will enhance the utility of ancient and low-copy-number DNA as a tool for closing forensic cases or in reconstructing the evolutionary history of humans and other organisms.
A Flexible External Paging Interface (1993)

: In this paper we describe an aspect of the Spring virtual memory system that was influenced by the distributed object-oriented architecture of Spring. The virtual memory system supports external pagers like those provided in the MACH ® operating system, yet the architecture is more flexible and provides better caching opportunities than is possible in other systems. A novel aspect of the architecture is the separation of the memory abstraction from the interface that provides the paging operations. This separation provides considerable caching opportunities in our file system, and it facilitates our extensible stackable file system architecture. The virtual memory architecture described in this paper is implemented and has been in use for over three years as part of the experimental Spring operating system. A Sun Microsystems, Inc. Business M/S 29-01 2550 Garcia Avenue Mountain View, CA 94043 email addresses: yousef.khalidi@eng.sun.com michael.nelson@eng.sun.com 2 A Flexible ...
Identification of novel regions of altered DNA copy number in small cell lung tumors (1995)

Identification of the genetic alterations that occur in tumors is an important approach to understanding tumorigenesis. We have used comparative genomic hybridization (CGH), a novel molecular cytogenetic method, to identify the gross DNA copy number changes that commonly occur in small cell lung cancer (SCLC). We analyzed ten SCLC tumors (seven primary tumors and three metastases) from eight patients. We found frequent increases in DNA copy number on chromosome arms 5p, 8q, 3q, and Xq and frequent decreases in copy number on chromosome arms 3p, 17p, 5q, 8p, 13q, and 4p. The increase in copy number at 8q24 (MYC) and decreases at 17p13 (TP53), 13q14 (RB), and 3p have previously been identified in SCLC with other methods. Many of the other regions in which we detected common copy number changes have not been reported to be regions of common alteration in SCLC tumors. Comparison of copy number changes between a primary tumor and a metastasis from the same patient showed that they were more closely related to each other than to any of the other tumors. The results of direct CGH analysis of SCLC tumors reported here confirm the existence of copy number changes that we identified previously by using cell lines.
Dynamic Rheology for the Prediction of Surgical Outcomes in Autologous Fat Grafting (2017)

Therapeutic, V.
Nerve Excitation Properties in Magnetic Stimulation by Trapezoidal Magnetic Fields (1994)

This study focuses on the properties of nerve excitation elicited by time-varying magnetic fields. We analyzed the effects of trapezoidal magnetic fields on the nerve transmembrane potential. Nerve excitation processes were stimulated while changing the rise time, the duration of the plateau, and the falling time of the trapezoidal signal. The results show that the exciting point and the threshold for nerve excitation change with the temporal behavior of the magnetic field, even when the stimulating intensity remains the same.
Ballads (1989)

A ballad is a narrative song, normally characterised by the recitation in simple language of a story which is presented through dialogue and described action. Traditionally the ballad was composed anonymously and passed on from one singer to another; singers often adapted both the dialect and the narrative details of ballads, so they tend to survive in several different versions. The process of collecting and publishing ballads began in the late sixteenth century. Some of the ballads collected in the next hundred years were medieval in origin, but they were printed in a form which was then contemporary. Printing inevitably inhibits further development, because the oral mode of transmission, which allows for continuous development of the ballad, gives way to the fixity of print. Eventually the printed form of ballads encouraged the composition of literary ballads, poems which imitate the conventions of authentic ballads; Coleridge's Rime of the Ancient Mariner and Wilde's Ballad of Reading Gaol are among the best examples of literary ballads.
Investigation of the Oligomerization of Light Olefins on Ion Exchange Resin Catalyst (2010)

The requirements against the quality of motor fuels were rendered more rigorous in the past decade; the most important ones were the major reductions of the sulphur and aromatic content. Besides the changing of the regulation of the motor fuels, the harmful material emission of the Otto- and Diesel-engine built vehicles was also limited significantly. These effects make necessary the greater use of the environmentally friendly, relatively “clean burning”, technically heteroatom- and aromatic free, great n- and i-paraffinic containing blending components in the case of gasoline and diesel gas oils. During our experimental work we studied the possibilities of producing motor fuel blending components like these blending components with the oligomerization of the olefin content of light FCC-naphtha – assuming later industrial application – on Amberlyst-15 acidic ion exchange resin type catalyst. This topic is very important in a modern oil refinery, because different isoparaffin-rich motor fuel blending components can be produced (depending on the catalyst and the process parameters), and this technology can grow considerably the gasoline- and diesel gas oil flexibility of the oil refinery, too. The aim of our experiments was to produce motor fuel blending components with the oligomerization of C4-C6 olefins in light FCC-naphtha matrix. Working on the acidic ion exchange resin catalyst under different process parameters (T: 80–130 °C, P: 15–30 bar, LHSV: 0.5–3.0 h-1) we determined that in the studied process parameter range in preferable case (T: 100–110 °C, P: 25–30 bar, LHSV 0.5–1.0 h-1) the liquid product yield was: greater than 95%, the conversion of olefins: 90–92%, the selectivity of C8-C11: 70–76%, while the selectivity of C12+: 24–30%.