gebhart/patrepeval huggingface dataset:
Sequence determinants of amyloid fibril formation (2003)

The establishment of rules that link sequence and amyloid feature is critical for our understanding of misfolding diseases. To this end, we have performed a saturation mutagenesis analysis on the de novo-designed amyloid peptide STVIIE (1). The positional scanning mutagenesis has revealed that there is a position dependence on mutation of amyloid fibril formation and that both very tolerant and restrictive positions to mutation can be found within an amyloid sequence. In this system, mutations that accelerate beta-sheet polymerization do not always lead to an increase of amyloid products. On the contrary, abundant fibrils are typically found for mutants that polymerize slowly. From these experiments, we have extracted a sequence pattern to identify amyloidogenic stretches in proteins. The pattern has been validated experimentally. In silico sequence scanning of amyloid proteins also supports the pattern. Analysis of protein databases has shown that highly amyloidogenic sequences matching the pattern are less frequent in proteins than innocuous amino acid combinations and that, if present, they are surrounded by amino acids that disrupt their aggregating capability (amyloid breakers). This study provides the potential for a proteome-wide scanning to detect fibril-forming regions in proteins, from which molecules can be designed to prevent and/or disrupt this process.
Benzo[<i>a</i>]pyrene Regulates Osteoblast Proliferation through an Estrogen Receptor-Related Cyclooxygenase-2 Pathway (2004)

Polycyclic aromatic hydrocarbons (PAHs) have been known as a kind of xenoestrogen. Benzo[a]pyrene, a PAH present in tobacco smoke and tar, has been implicated in the induction of cell proliferation as well as tumors including osteosarcoma. Nevertheless, the literature about the action of benzo[a]pyrene on the bone system is rare. It has been identified that osteoblasts owned the estrogen receptors and estrogen could modulate the osteoblast proliferation. In this study, we found that benzo[a]pyrene was capable of increasing the cell proliferation in cultured rat osteoblasts, human osteosarcoma cell line (MG-63), and estrogen sensitive human cell line (MCF-7) but not in the human estrogen receptor negative cell line (MDA-MB-231). This benzo[a]pyrene-induced osteoblast proliferation could be inhibited by the estrogen receptor antagonist ICI182780 and tamoxifen, PD98059 [extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) inhibitor], and LY294002 [phosphatidylinositol 3-kinase (PI3K) inhibitor] but not alpha-naphthoflavone (aryl hydrocarbon receptor antagonist) and SB203580 (p38 MAPK inhibitor). Western blot analysis showed that benzo[a]pyrene could induce the phosphorylation of ERK1/2 and Akt (PI3K downstream effector) in osteoblasts. The proliferating cell nuclear antigen protein levels in nuclear fraction of osteoblasts were also increased by benzo[a]pyrene. Moreover, cyclooxygenase-2 (COX-2), but not COX-1, expression could be induced in osteoblasts under benzo[a]pyrene treatment. Its upregulation was associated with the induction of prostaglandin E(2) (PGE(2)). COX-2 inhibitors NS398 and aspirin are capable of inhibiting the benzo[a]pyrene-induced osteoblast proliferation. These results indicate that benzo[a]pyrene may modulate the osteoblast proliferation through activation of COX-2 protein.
A Software-Defined Radio System for Backscatter Sensor Networks (2008)

Backscatter radio is proposed for sensor networks. In that way, the transmitter for each sensor is simplified to a transistor connected to an antenna and therefore, the cost for each sensor's communicator becomes negligible, while energy used for wireless communication per sensor is minimized. A software-defined transceiver is built to transmit a carrier, receive the reflections from various sensors and extract their transmitted messages. This work presents a thorough model of the backscatter radio link, the system architecture and a set of data extraction techniques for each sensor's information, testing in practice a sensor communicating through backscatter at a range of approximately 15 meters indoors, with 5 milliwatt transmission power at 10 bits per second. This work highlights the idiosyncrasies of the backscatter channel and provides a new communication perspective in the fertile area of scalable sensor networks, especially when low bit-rate, ultra-low cost sensors are required.
The effect of ephedrine plus caffeine on plasma lipids and lipoproteins during a 4.2 MJ/day diet. (1994)

The effect of treatment with a combination of ephedrine and caffeine during eight weeks' energy restriction (4.2 MJ/day) on the fasting plasma lipid profile was studied in obese women. Thirty-two women were randomly assigned to either ephedrine 20 mg plus caffeine 200 mg (EC) or placebo three times per day. Weight loss (7.8 +/- 0.63 kg) and loss of fat mass (6.2 +/- 0.49 kg) did not differ significantly between the two groups. Total cholesterol concentration declined from baseline to week 8 (0.58 +/- 0.13 mmol/l) with no significant difference between the two groups. HDL-cholesterol concentration was decreased by 0.14 +/- 0.03 mmol/l at week 4 and week 8 in the placebo group (P < 0.05) but remained unchanged in the EC group. No effect of dieting was seen on plasma triglyceride concentration in the placebo group but a decrease was observed in the EC group at week 8. It is suggested that EC combinations abolish the decline in HDL-cholesterol during active weight loss due to the beta-agonistic properties of ephedrine.
The President's Fiscal Year 1992 Budget: Almost Quiet on the Budgetary Front (1991)

The Fiscal Year 1992 Budget was prepared under unusual conditions. The Persian Gulf War, the piesident's disinterest in domestic issues, the bitter debate over the fiscal year 1991 budget, and the upcoming re‐apportionment all combined to make this year atypical The result is a document in which the most substantial change is the cover—a patriotic red, white, and blue Inside, it uses the “one book” format of fiscal year 1991 The budget examines different interpretations of the budget deficit, implements major changes in budgetary accounting, and outlines assumptions made in preparing the budget. Overall, the budget document consolidates the gains made in 1990
Usefulness of the Total Thrombus‐Formation Analysis System (T‐<scp>TAS</scp>) in the diagnosis and characterization of von Willebrand disease (2016)

The T-TAS appears to be sensitive mainly to plasma VWF concentration and the presence of large multimers. Failure of the PL and AR chips to become occluded points to a lack of large VWF multimers, or type 1 VWD with VWF levels below 10 U dL<sup>-1</sup> . Although the T-TAS does not assure a precise VWD diagnosis, it does point us in the right direction, and thus seems a useful global preliminary test.
A bulky pyridinylfluorene-fuctionalizing approach to synthesize diarylfluorene-based bipolar host materials for efficient red, green, blue and white electrophosphorescent devices (2013)

By incorporating electron-transporting pyridine and hole-transporting fluorene moieties into a nonplanar 3-dimensional molecule, via the sp3-hybridized carbon linkage by Friedel–Crafts reaction, three novel donor–acceptor (p–n) bipolar host materials 9-(pyridin-2-yl)-3-(9-(pyridin-2-yl)-fluoren-9-yl)-carbazole (CzPy-PyFM), 3,6-bis(9-(pyridine-2-yl)-fluoren-9-yl)-9-(pyridin-2-yl)-carbazole (CzPy-DPyFM) and 5,5′′-bis(9-(pyridin-2-yl)-fluoren-9-yl-)-2,2′:5′,2′′-terthiophene (T3DPyFM) have been synthesized successfully. These pyridinylfluorene end-capped materials with large steric hindrance have been applied as efficient universal hosts for blue, green and red PhOLEDs. The blue, green, and red PhOLEDs hosted by highly thermally stable CzPy-PyFM exhibit the maximum external quantum efficiencies (EQEs) of 10.49%, 11.04%, and 6.50%, respectively. On this basis, the single-emissive-layer three-color and all-phosphor CzPy-PyFM-based white PhOLEDs have been fabricated, which acquire a maximum current efficiency of 10.4 cd A−1, a power efficiency of 10.2 lm W−1 and an EQE of 5.58%. This is the first example of the diarylfluorenes as universal hosts for full-color RGB PhOLEDs as well as white PhOLEDs.
The physical limits of computing (2002)

Many of the fundamental limits on information processing, from thermodynamics, relativity, and quantum mechanics, are only a few decades away. Novel physically motivated computing paradigms, such as reversible computing and quantum computing, may help in certain ways, but even they remain subject to some basic limits. One can arrive at several firm conclusions regarding upper bounds on the limits of computing. In this article, the author only reviews the fundamental technology-independent limits.
The design of a fully integrated graphics system (1988)

The architecture and design of a CMOS chip implementing a medium-resolution graphics system are described. The chip, requiring no external support logic, outputs analog RGB signals at a 40-MHz pixel rate and directly controls a bit-map video RAM (VRAM) memory array. Scan rates and display formats are completely programmable. Pixels stored in the 1 K*1 K bit map can be any of 16 colors taken from a 4096-color palette. The chip can be directly interfaced to most common microprocessors. A 6.7-MIPS (million-instruction-per-second) internal reduced instruction set computer (RISC) CPU directly implements high-level graphics commands. The chip achieves a maximum draw speed of 10 million pixels/s. Designed in a Lisp machine environment, the 100000-transistor chip is implemented in 1.8- mu m CMOS and contains standard cells, RAM, ROM, a color table, and three four-bit current-steered digital-to-analog converters (DACs).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Composite 3D-printed metastructures for low-frequency and broadband vibration absorption (2016)

Architected materials that control elastic wave propagation are essential in vibration mitigation and sound attenuation. Phononic crystals and acoustic metamaterials use band-gap engineering to forbid certain frequencies from propagating through a material. However, existing solutions are limited in the low-frequency regimes and in their bandwidth of operation because they require impractical sizes and masses. Here, we present a class of materials (labeled elastic metastructures) that supports the formation of wide and low-frequency band gaps, while simultaneously reducing their global mass. To achieve these properties, the metastructures combine local resonances with structural modes of a periodic architected lattice. Whereas the band gaps in these metastructures are induced by Bragg scattering mechanisms, their key feature is that the band-gap size and frequency range can be controlled and broadened through local resonances, which are linked to changes in the lattice geometry. We demonstrate these principles experimentally, using advanced additive manufacturing methods, and inform our designs using finite-element simulations. This design strategy has a broad range of applications, including control of structural vibrations, noise, and shock mitigation.
Monoclonal antibody 14C5 targets integrin αvβ5 (2008)

This study identifies and characterizes the antigen recognized by monoclonal antibody (mAb) 14C5. We compared the expression of antigen 14C5 with the expression of eight integrin subunits (alpha1, alpha2, alpha3, alphav, beta1, beta2, beta3, and beta4) and three integrin heterodimers (alphavbeta3, alphavbeta5, and alpha5beta1) by flow cytometry. Antigen 14C5 showed a similar expression to alphavbeta5 in eight different epithelial cancer cell lines (A549, A2058, C32, Capan-2, Colo16, HT-1080, HT-29, and SKBR-3). Specific binding of P1F6, an anti-alphavbeta5 specific antibody, was blocked by mAb 14C5. After transient expression of alphavbeta5 in 14C5-negative Colo16 cells, mAb 14C5 was able to bind a subpopulation of alphavbeta5-positive cells. We evaluated the tissue distribution of the 14C5 antigen in colon (n = 20) and lung (n = 16) cancer tissues. The colon carcinoma cells stained positive for 14C5 in 50% of tumors analyzed, whereas bronchoalveolar lung carcinoma and typical carcinoid were not positive for the antigen. More common types of non-small cell lung cancer, i.e., squamous (n = 5) and adenocarcinoma (n = 3), stained positive in 2 of 5 squamous carcinomas and in 1 of 3 investigated adenocarcinoma. Colon (95%) and lung (50%) carcinoma tissues showed extensive expression of antigen 14C5 in the stroma surrounding the tumor cells and on the membrane of the adjacent fibroblasts. We show for the first time that mAb 14C5 binds the vascular integrin alphavbeta5, suggesting that mAb 14C5 can be used as a screening agent to select colon and lung cancer patients that are eligible for anti-alphavbeta5-based therapies.
Asymmetric Hydrogenation of 2,4-Dioxo Esters: Selective Synthesis of 2-Hydroxy-4-oxo Esters and Direct Access to Chiral 2-Hydroxy-4-butyrolactones (1999)

2,4-Dioxoesters 1a–c are selectively converted into optically active 2-hydroxy-4-oxoesters 2a–c by hydrogenation with chiral rhodium-aminophosphane-phosphinite catalysts (82–88% ee) or ruthenium-bisphosphane catalysts (52–67% ee). Direct one-pot hydrogenation of 2,4-dioxoesters 1a–c to 2-hydroxy-4-butyrolactones 4a–c proceeds in high yields; catalytic activities, chemo-, dia-, and enantioselectivities are strongly dependant upon the nature of the substrate and the catalyst.
Report on Magnesium in Fertilizers (1936)

Journal Article Report on Magnesium in Fertilizers Get access John B Smith John B Smith Associate Referee Agricultural Experiment Station, Kingston, R. I Search for other works by this author on: Oxford Academic Google Scholar Journal of Association of Official Agricultural Chemists, Volume 19, Issue 2, 1 May 1936, Pages 288–301, https://doi.org/10.1093/jaoac/19.2.288 Published: 20 February 2020
A Mobile Phone Device as a Biometrics Authentication Method for an ATM Terminal (2015)

The use of mobile phone devices is expanding rapidly and they become essential tools that offer competitive business advantages in today's growing world of global computing environments. A Mobile phone device is a suitable tool for a multifactor authentication that could provide powerful and easy to use authentication device to access any service securely such as an ATM terminal as well as would increase the level of protection for critical and sensitive information. In this paper, we present a protocol that provides more secure ATM authentication using biometrics (fingerprint or face) on a mobile phone device under the restriction that no changes can be made to the existing physical infrastructure. Furthermore, we give an overview of the current ATM authentication methods utilizing mobile devices as a factor in the authentication process. Moreover, we outline a high level security analysis for the proposed authentication protocol.
Emerging therapies for sepsis and septic shock. (1998)

Despite advances in antimicrobial therapy and medical support, septic shock remains a leading cause of death. Emerging adjunctive therapy for septic shock can be divided into those directed against bacterial components, those directed against host-derived inflammatory-mediators and those designed to limit tissue damage. All trials of new adjunctive therapies for sepsis and septic shock conducted to data have failed to show efficacy. Therapies against endotoxin, tumour necrosis factor, interleukin-1 and platelet activating factor did not reduce mortality. Future effective therapies will probably use combination of agents depending upon the nature of the infection and the type of patient.
Production of 5,8,11‐<i>cis</i>‐eicosatrienoic acid by a Δ12‐desaturase‐defective mutant of<i>Mortierella alpina</i> 1S‐4 (1992)

A mutant defective in Δ12‐desaturase of an arachidonic‐acid producing fungus, Mortierella alpina 1S‐4, was shown to be a novel potent producer of Mead acid (5,8,11‐ cis ‐eicosatrienoic acid, 20:3ω9). The fungus produced several fatty acids of the n‐9 family, i.e. , 6,9‐ cis ‐octadecadienoic acid (18:2ω9), 8,11‐ cis ‐eicosadienoic acid (20:2ω9) and 20:3ω9. Significantly high levels of these fatty acids were produced during growth at low temperatures (12–20°C). On submerged cultivation at 20°C for 10 days in a 5‐L fermenter containing 2% glucose plus 1% yeast extract (pH 6.0), the production of 20:3ω9 reached ca. 0.8 g/L (56 mg/g dry mycelia), accounting for 15% (by wt) of the total mycelial fatty acids. The other major fatty acids were palmitic acid (6%), stearic acid (11%), oleic acid (45%), 18:2ω9 (12%) and 20:2ω9 (3%). Studies on the distribution of fatty acids among lipid classes showed that, irrespective of the growth temperature employed (12–28°C), ca. 70% (by mol) of 20:3ω9 was present in the triglyceride and the remainder in the phospholipid fraction, especially in phosphatidylcholine (PC). When the fungus was grown at 12°C, the proportion of 20:3ω9 in the PC fraction was ca. 55%.
Spectrophotometric determination of vitamin K compounds (1977)

<p>In this paper we study the stability and L<sub>2</sub>-gain properties of a class of hybrid systems that exhibit linear flow dynamics, periodic time-triggered jumps and arbitrary nonlinear jump maps. This class of hybrid systems is relevant for a broad range of applications including periodic event-triggered control, sampled-data reset control, sampled-data saturated control, and certain networked control systems with scheduling protocols. For this class of continuous-time hybrid systems we provide new stability and L<sub>2</sub>-gain analysis methods. Inspired by ideas from lifting we show that the stability and the contractivity in L<sub>2</sub>-sense (meaning that the L<sub>2</sub>-gain is smaller than 1) of the continuous-time hybrid system is equivalent to the stability and the contractivity in l<sub>2</sub>-sense (meaning that the l<sub>2</sub>-gain is smaller than 1) of an appropriate discrete-time nonlinear system. These new characterizations generalize earlier (more conservative) conditions provided in the literature.We show via a reset control example and an event- triggered control application, for which stability and contractivity in L<sub>2</sub>-sense is the same as stability and contractivity in l<sub>2</sub>-sense of a discrete-time piecewise linear system, that the new conditions are significantly less conservative than the existing ones in the literature. Moreover, we show that the existing conditions can be reinterpreted as a conservative l<sub>2</sub>-gain analysis of a discretetime piecewise linear system based on common quadratic storage/ Lyapunov functions. These new insights are obtained by the adopted lifting-based perspective on this problem, which leads to computable l<sub>2</sub>-gain (and thus L<sub>2</sub>-gain) conditions, despite the fact that the linearity assumption, which is usually needed in the lifting literature, is not satisfied.</p>
Ruthenium(ii) polypyridyl complexes as carriers for DNA delivery (2011)

Two novel water soluble ruthenium(II) complexes [Ru(bpy)(2)(bqbg)](2+) and [Ru(phen)(2)(bqbg)](2+) have been structurally characterized and their DNA condensation activity, cytotoxicity, and cellular uptake studies of DNA condensates as potential non-viral DNA carriers were evaluated.
Systemic regulation of starvation response in <i>Caenorhabditis elegans</i> (2009)

When the supply of environmental nutrients is limited, multicellular animals can make both physiological and behavioral changes so as to cope with nutrient starvation. Although physiological and behavioral effects of starvation are well known, the mechanisms by which animals sense starvation systemically remain elusive. Furthermore, what constituent of food is sensed and how it modulates starvation response is still poorly understood. In this study, we use a starvation-hypersensitive mutant to identify molecules and mechanisms that modulate starvation signaling. We found that specific amino acids could suppress the starvation-induced death of gpb-2 mutants, and that MGL-1 and MGL-2, Caenorhabditis elegans homologs of metabotropic glutamate receptors, were involved. MGL-1 and MGL-2 acted in AIY and AIB neurons, respectively. Treatment with leucine suppressed starvation-induced stress resistance and life span extension in wild-type worms, and mutation of mgl-1 and mgl-2 abolished these effects of leucine. Taken together, our results suggest that metabotropic glutamate receptor homologs in AIY and AIB neuron may modulate a systemic starvation response, and that C. elegans senses specific amino acids as an anti-hunger signal.
Synthesis and liquid chromatographic evaluation of some chiral derivatizing agents for resolution of amine enantiomers (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and liquid chromatographic evaluation of some chiral derivatizing agents for resolution of amine enantiomersC. Randall. Clark and Jeffrey M. BarksdaleCite this: Anal. Chem. 1984, 56, 6, 958–962Publication Date (Print):May 1, 1984Publication History Published online1 May 2002Published inissue 1 May 1984https://pubs.acs.org/doi/10.1021/ac00270a022https://doi.org/10.1021/ac00270a022research-articleACS PublicationsRequest reuse permissionsArticle Views239Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
DESIGN AND DYNAMICS OF A SHAPE MEMORY ALLOY WIRE BUNDLE ACTUATOR (1999)

In this paper, the design and dynamics of a new Shape Memory Alloy (SMA) actuator that possesses impressive payload lifting capabilities are presented. This actuator consists of 48 SMA wires mechanically bundled in parallel forming one powerful muscle. The new actuator can lift up to 100 lbs., which is approximately 300 times its weight. This actuator was tested in open loop experiments with different weights and different inputs, such as step, ramp, sinusoid, and half sinusoid, and its dynamic characteristics were evaluated. Of special importance is the observed unpredictability of the actuator&apos;s response when low voltages are applied. This characteristic suggests possible chaotic behavior of the actuator, potentially causing control difficulties in fine and high accuracy tasks. 1. Introduction Robotic systems have been proposed to perform important tasks in the nuclear industry such as hazardous waste removal and decommissioning of nuclear sites [1, 2]. Since these tasks occur in a...
Application of Curable Resin-Coated Proppants (1992)

Summary Laboratory investigation of the interactions between fracturing fluids and resin-coated proppants (RCP's) revealed (among other conclusions) that RCP's are incompatible with oxidizing breakers. Areas covered included RCP effect on fluid rheology, fluid relationship to RCP strength, theoretical study of required RCP strengths to prevent flowback, and experimental measurement to establish minimum strength.