gebhart/patrepeval huggingface dataset:
Pathophysiologic Importance of E– and L–Selectin for Neutrophil–Induced Liver Injury During Endotoxemia in Mice (2000)

Neutrophils can cause parenchymal cell injury in the liver during ischemia-reperfusion and endotoxemia. Neutrophils relevant for the injury accumulate in sinusoids, transmigrate, and adhere to hepatocytes. To investigate the role of E- and L-selectin in this process, C3Heb/FeJ mice were treated with 700 mg/kg galactosamine and 100 microgram/kg endotoxin (Gal/ET). Immunogold labeling verified the expression of E-selectin on sinusoidal endothelial cells 4 hours after Gal/ET injection. In addition, Gal/ET caused up-regulation of Mac-1 (CD11b/CD18) and shedding of L-selectin from circulating neutrophils. Gal/ET induced hepatic neutrophil accumulation (422 +/- 32 polymorphonuclear leukocytes [PMN]/50 high power fields [HPF]) and severe liver injury (plasma alanine transaminase [ALT] activities: 4,120 +/- 960 U/L; necrosis: 44 +/- 3%) at 7 hours. Treatment with an anti-E-selectin antibody (3 mg/kg, intravenously) at the time of Gal/ET administration did not significantly affect hepatic neutrophil accumulation and localization. However, the anti-E-selectin antibody significantly attenuated liver injury as indicated by reduced ALT levels (-84%) and 43% less necrotic hepatocytes. In contrast, animals treated with an anti-L-selectin antibody or L-selectin gene knock out mice were not protected against Gal/ET-induced liver injury. However, E-, L-, and P-selectin triple knock out mice showed significantly reduced liver injury after Gal/ET treatment as indicated by lower ALT levels (-65%) and reduced necrosis (-68%). Previous studies showed that circulating neutrophils of E-selectin-overexpressing mice are primed and activated similar to neutrophils adhering to E-selectin in vitro. Therefore, we conclude that blocking E-selectin or eliminating this gene may have protected against Gal/ET-induced liver injury in vivo by inhibiting the full activation of neutrophils during the transmigration process.
Development of a physically-based ocean bottom classification analysis system using multibeam sonar backscatter (2002)

A physically based mathematical model has been developed to analyze the backscatter intensity from wide swath multibeam sonar systems in order to classify the ocean bottom roughness and sediment type. The input to the model is mosaiced multibeam data corrected for transmission loss (spreading loss and range attenuation), spot size, and local beam-to-bottom incidence angle. Modern image analysis techniques, including geometry driven diffusion and wavelet analysis, are used to segment the corrected, mosaiced backscatter image into regions of limited homogeneity. For each region the authors construct an intensity versus scatter angle curve, which characteristic of the scattering sediment. The curve is then analyzed by a model of backscatter acoustics that takes into account effects of small and large scale roughness of the water-sediment interface as well as volume scattering within the sediment. This analysis yields parameters which characterize the sediment type.
Polyelectrolyte-modified multi-walled carbon nanotubes for the adsorption of chromium(vi) (2011)

Multi-walled carbon nanotubes (MWNTs) are noncovalently wrapped by a cationic polyelectrolyte of poly(diallyldimethylammonium chloride) (PDDA) via π-π interactions between the unsaturated impurity in PDDA and MWNTs. The modified PDDA-MWNTs are characterized by FT-IR and surface charge analysis. The PDDA-MWNTs are packed into a mini-column and used for the adsorption of Cr(vi) in a sequential injection system with detection by electrothermal atomic absorption spectrometry (ETAAS). A 32% improvement on the adsorption efficiency of Cr(vi) by the PDDA-MWNTs at pH 6 is achieved with respect to that obtained by the bare MWNTs. The retained Cr(vi) is afterwards recovered by using 80 μL of ammonium nitrate solution (0.1 mol L-1) as stripping reagent producing an elution efficiency of 81%. The presence of a certain amount of Cr(iii) within a Cr(iii)/Cr(vi) concentration ratio of 6 : 1 causes no interfering effect for the adsorption of Cr(vi) by the PDDA-MWNTs mini-column. A sample loading volume of 1000 μL creates an enrichment factor of 8.6, along with a detection limit of 0.016 μg L-1 (3σ, n = 7) and a RSD of 3.9% (0.5 μg L-1) within a linear calibration range of 0.05-1.5 μg L-1. The procedure has been validated by analyzing a certified reference material of GBW08608 and a series of water samples.
Toward a verified relational database management system (2010)

We report on our experience implementing a lightweight, fully verified relational database management system (RDBMS). The functional specification of RDBMS behavior, RDBMS implementation, and proof that the implementation meets the specification are all written and verified in Coq. Our contributions include: (1) a complete specification of the relational algebra in Coq; (2) an efficient realization of that model (B+ trees) implemented with the Ynot extension to Coq; and (3) a set of simple query optimizations proven to respect both semantics and run-time cost. In addition to describing the design and implementation of these artifacts, we highlight the challenges we encountered formalizing them, including the choice of representation for finite relations of typed tuples and the challenges of reasoning about data structures with complex sharing. Our experience shows that though many challenges remain, building fully-verified systems software in Coq is within reach.
Ex vivo removal of serum igg in a patient with colon carcinoma.Some biochemical, immunological and histological observations (1978)

Plasma of a patient with metastatic colon carcinoma was perfused over Formalin and heat-killed S. aureus, in an extracorporeal filtration apparatus, in order to nonspecifically remove IgG and its complexes. Twenty ex vivo absorption procedures were done, over a five-month period, with a minimum of discomfort to the patient. Extracorporeal perfusion of plasma on S. aureus effectively reduced the levels of IgG and immune complexes in the perfused plasma. The nonspecific removal of IgG resulted in 1) slight biochemical alterations in the serum, 2) a transient reduction in the serum blocking activity and appearance of complement-dependent serum cytotoxicity, 3) an increase in the serum IgM levels, 4) a transient increase in the Ig surface-bearing lymphocytes and a decrease in "E" rosetting lymphocytes in the first 24-48 hours postperfusion, particularly during the early treatments, 5) an improvement in general condition of the patient and decrease in tumor size, and 6) histological changes in the tumor consistent with tumor destruction. The potential problems and clinical applications of procedures involving ex vivo specific or nonspecific immunoabsorbents are discussed.
Comparative Analysis of GaAs/LDMOS/GaN High Power Transistors in a Digital Predistortion Amplifier System (2006)

Silicon LDMOS transistors have long been employed as the technology of choice for high power amplifiers in cellular infrastructure equipment. With each successive generation, improvements have been achieved in the areas of power, efficiency, linearity and reliability. As device performance approaches ideal, the focus for improvements has shifted to circuit based techniques and alternate device technologies such as GaAs, and lately GaN. Employing these technologies successfully in high power amplifier designs affords unique opportunities and challenges. In addition, as distortion correction techniques migrate towards the digital domain, new algorithms must address the unique properties of each technology. In this paper a comparative analysis of GaAs, LDMOS, and GaN devices are performed in a digitally predistorted amplifier system.
Design and Syntheses of Permethyl Ningalin B Analogues: Potent Multidrug Resistance (MDR) Reversal Agents of Cancer Cells (2010)

A series of novel N-arylalkyl-3,4-diaryl-substituted pyrrole-2,5-diones were synthesized. They exhibited promising P-gp modulating activity in a P-gp overexpressing breast cancer cell line (LCC6MDR). Compound 6 (with three methoxy groups at D-ring) displayed the highest P-gp modulating activity. 6 at 1 microM can sensitize LCC6MDR cells toward paclitaxel by 18.2-fold. Interestingly, a synergy on modulating P-gp was noted when 6 and 25 were used together (fractional inhibitory concentration index FICI = 0.42). Combination of 6 (0.5 microM) and 25 (0.5 microM) resulted in a 66-fold sensitization of LCC6MDR cells toward paclitaxel. They also reversed P-gp mediated doxorubicin (DOX) and vincristine resistance. Kinetic characterization suggests that permethyl ningalin B analogues likely act as a noncompetitive inhibitor of P-gp-mediated DOX transport (K(i) = 5.4-5.8 microM). The present study demonstrates that synthetic analogues of permethyl ningalin B can be employed as effective and safe modulators of P-gp-mediated drug resistance in cancer cells.
Precancer of the prostate. (1998)

The evolution of the malignant phenotype requires a set of genetic and epigenetic changes in sets of genes responsible for regulation of normal growth and cell death, of "social behaviour" and differentiation. The sum of these changes, not only the sequence, determines the malignancy as well as its grade. The probability of invasiveness shows a remarkable relationship to morphological changes, which in turn prove to be accompanied by a multitude of discrete molecular perturbations. Some of these can be characterized as functional, others as inductive with respect to their participation in the process. Since only the functional changes regulate malignant behaviour per se, it is an important task for future research to assemble a set of such changes, find markers for them and combine morphological and molecular indicators to achieve prognostically optimal scores. It should be emphasized, though, that rational use of such scores using biopsy samples as a source of information cannot be defined until biopsy strategies have been standardized and optimized.
A Study on the Peak Separation of Acetone and Acrolein Based on High-Performance Liquid Chromatography (HPLC) Method (2009)

To resolve the differentiation problem of acetone and acrolein in the analysis of carbonyls by high-performance liquid chromatography (HPLC), we investigated the optimum analytical conditions for their separation. Carbonyl compounds were collected by 2,4-dinitrophenylhydrazine (DNPH)-coated cartridges. We examined the influence of three experimental variables: temperature (25, 30, 40, 50 and 60 <TEX>${^{\circ}C}$</TEX>), flow rate (1.0 and 1.2 mL/min), and relative mobile phase composition (among acetonitrile, water and tetrahydrofuran). The experimental results revealed the optimum analytical condition of a flow rate of 1.2 mL/min, temperature of 32 <TEX>${^{\circ}C}$</TEX> and mobile phase composition of acetonitrile: water: tetrahydrofuran = 34 : 52.8 : 13.2. The analysis of indoor air composition indicated that acrolein and acetone comprised 11% and 42% of all aldehydes, respectively.
Computer-aided noise analysis of MESFET and HEMT mixers (1989)

A numerical approach to the noise analysis of MESFET and HEMT mixers of arbitrary topology is discussed. A qualitative picture of the complex physical mechanisms responsible for the generation of the intermediate frequency (IF) noise is outlined, and the corresponding computational algorithms are presented. The derivation of a noisy nonlinear model for the microwave FET is addressed, and it is shown that a satisfactory solution to this problem can be obtained by combining a conventional time-domain model with standard noise information. The method has been implemented in a computer program designed to work in conjunction with an existing general-purpose harmonic-balance simulator. An application is described in detail to demonstrate the excellent performance of this software tool.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
An Inducible Lentiviral Guide RNA Platform Enables the Identification of Tumor-Essential Genes and Tumor-Promoting Mutations In Vivo (2015)

The CRISPR/Cas9 technology enables the introduction of genomic alterations into almost any organism; however, systems for efficient and inducible gene modification have been lacking, especially for deletion of essential genes. Here, we describe a drug-inducible small guide RNA (sgRNA) vector system allowing for ubiquitous and efficient gene deletion in murine and human cells. This system mediates the efficient, temporally controlled deletion of MCL-1, both in vitro and in vivo, in human Burkitt lymphoma cell lines that require this anti-apoptotic BCL-2 protein for sustained survival and growth. Unexpectedly, repeated induction of the same sgRNA generated similar inactivating mutations in the human Mcl-1 gene due to low mutation variability exerted by the accompanying non-homologous end-joining (NHEJ) process. Finally, we were able to generate hematopoietic cell compartment-restricted Trp53-knockout mice, leading to the identification of cancer-promoting mutants of this critical tumor suppressor.
A Distributed Cognitive Approach Towards Internet Communication using F-Test (2016)

Internet is supposed to be a powerful multi-purpose tool for information searching. A number of people of different ages; from teenagers to old persons use it for sharing ideas, knowledge, experiences, cultures and communication. Internet has brought technological advancement in academics where the mode and medium of studies and research have almost changed. The faculty and students use more of internet than any other source for their teaching and studies purpose. The aim of this paper is to statistically test the significant difference between thoughts of faculty towards internet usage using F-Test. The research is conducted using a structured questionnaire according to five Point Likert-scales. More than hundred samples have been collected from six different reputed institutions including both male and female faculty in Sirsa District. By Applying F-Test on the samples reveals the scatteredness in thoughts of male faculty and female faculty towards the use of internet in their social, academic and personal lives.
Bacterial meningitis in the United States, 1978 through 1981. The National Bacterial Meningitis Surveillance Study (1985)

From 1977 to 1981, 18,642 cases of bacterial meningitis were reported to the Centers for Disease Control. We analyzed data from 27 states with full participation from 1978 through 1981.<i>Hemophilus influenzae</i>was the most frequent cause of bacterial meningitis (48.3%), followed by<i>Neisseria meningitidis</i>(19.6%) and<i>Streptococcus pneumoniae</i>(13.3%). Overall attack rates for males were greater than for females (3.3 v 2.6 cases per 105 population per year). Attack rates were highest in children under 1 year of age (76.7 per 105 population per year). Case-fatality ratios were highest for gram-negative and miscellaneous causes of bacterial meningitis (33.7%) and lowest for meningitis caused by<i>H influenzae</i>(6.0%).<i>Neisseria meningitidis</i>and<i>S pneumoniae</i>meningitis occurred preponderantly during the winter, while<i>H influenzae</i>meningitis had peak activity in the spring and fall. Ampicillin resistance among<i>H influenzae</i>increased from 18.7% in 1978, to 23.9% in 1981. Serogroup<i>B Neisseria meningitidis</i>was the most common serogroup identified during the reporting period (51.1%), followed by serogroup C (22.3%), serogroup Y (5.8%), and serogroup A (4.7%) infections. (<i>JAMA</i>1985;253:1749-1754)
NetLord (2011)

Providers of "Infrastructure-as-a-Service" need datacenter networks that support multi-tenancy, scale, and ease of operation, at low cost. Most existing network architectures cannot meet all of these needs simultaneously. In this paper we present NetLord, a novel multi-tenant network architecture. NetLord provides tenants with simple and flexible network abstractions, by fully and efficiently virtualizing the address space at both L2 and L3. NetLord can exploit inexpensive commodity equipment to scale the network to several thousands of tenants and millions of virtual machines. NetLord requires only a small amount of offline, one-time configuration. We implemented NetLord on a testbed, and demonstrated its scalability, while achieving order-of-magnitude goodput improvements over previous approaches.
InGaAsSb/InP Double Heterojunction Bipolar Transistors Grown by Solid-Source Molecular Beam Epitaxy (2007)

This letter investigates the dc characteristics of a double heterojunction bipolar transistor (DHBT) with a compressively strained InGaAsSb base, which is grown by solid-source molecular beam epitaxy. The novel InP/InGaAsSb HBT has a lower base/emitter (B/E) junction turn-on voltage, a lower offset voltage, and a junction ideality factor closer to unity than the conventional InP/InGaAs composite collector DHBT. These characteristics are attributed to the transistor's type-I B/E junction and type-II base/collector junction, which facilitates carrier transport for low power, high current density, and high-speed operation. Heterojunction bipolar transistors (HBTs), InP/InGaAsSb, molecular beam epitaxy (MBE).
Structural and genetic analysis of the folding and function of T4 lysozyme (1996)

The combination of directed mutagenesis with high-resolution structure analysis has made it possible to systematically address fundamental questions of protein folding and stability. Here we briefly review some recent results in this area based on studies of the lysozyme of bacteriophage T4. Extended segments of the polypeptide chain can be substituted with alanine, suggesting that about 50%, or perhaps less, of the overall amino acid sequence protein is necessary to define the 3-dimensional structure of the protein. It is the internal residues that seem to be most important for folding and stability (although not necessarily for function). Substitutions within the core of the protein of large nonpolar side chains with smaller ones have been used to better understand the nature of hydrophobic stabilization. Mutants that produce the largest cavities within the protein tend to be most destabilizing, allowing the energy cost of cavity formation to be estimated. Small, nonpolar ligands bind within such cavities and restore some stability to the protein. Analogous, nonpolar ligands do not bind, however, providing evidence that water molecules do not bind with high occupancy within nonpolar cavities. In a further series of studies it has been possible to re-engineer the active site region of T4 lysozyme to change the catalytic mechanism of the enzyme.
Effects of oral doxycycline administration on histomorphometry and dynamics of subchondral bone in a canine model of osteoarthritis. (1996)

Doxycycline protects against joint breakdown in this OA model via inhibition of matrix metalloproteinases in articular cartilage, rather than through an effect on subchondral bone.
Coördination Compounds of Palladous Chloride<sup>1</sup> (1938)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCoördination Compounds of Palladous Chloride1M. S. Kharasch, R. C. Seyler, and Frank R. MayoCite this: J. Am. Chem. Soc. 1938, 60, 4, 882–884Publication Date (Print):April 1, 1938Publication History Published online1 May 2002Published inissue 1 April 1938https://doi.org/10.1021/ja01271a035RIGHTS & PERMISSIONSArticle Views1659Altmetric-Citations378LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (307 KB) Get e-Alerts Get e-Alerts
Toward Efficient Zn(II)-Based Artificial Nucleases (2004)

A series of cis-cis-triaminocyclohexane Zn(II) complex-anthraquinone intercalator conjugates, designed in such a way to allow their easy synthesis and modification, have been investigated as hydrolytic cleaving agents for plasmid DNA. The ligand structure comprises a triaminocyclohexane platform linked by means of alkyl spacers of different length (from C(4) to C(8)) to the anthraquinone group which may intercalate the DNA. At a concentration of 5 microM, the complex of the derivative with a C(8) alkyl spacer induces the hydrolytic stand scission of supercoiled DNA with a rate of 4.6 x 10(-6) s(-1) at pH 7 and 37 degrees C. The conjugation of the metal complex with the anthraquinone group leads to a 15-fold increase of the cleavage efficiency when compared with the anthraquinone lacking Zn-triaminocyclohexane complex. The straightforward synthetic procedure employed, allowing a systematic change of the spacer length, made possible to gain more insight on the role of the intercalating group in determining the reactivity of the systems. Comparison of the reactivity of the different complexes shows a remarkable increase of the DNA cleaving efficiency with the length of the spacer. In the case of too-short spacers, the advantages due to the increased DNA affinity are canceled due to the incorrect positioning of the reactive group, thus leading to cleavage inhibition.
Meningoencephalitis Due to Hyphae-forming<i>Cryptococcus neoformans</i> (1971)

A patient with lymphocytic leukemia died of meningoencephalitis due to hyphae-forming Cryptococcus neoformans. These forms were seen only in fresh preparations of cerebrospinal fluid and were lost immediately after culture on artificial media. The apparent rarity of these forms and their significance are discussed.
Differences in genome-wide repeat sequence instability conferred by proofreading and mismatch repair defects (2015)

Mutation rates are used to calibrate molecular clocks and to link genetic variants with human disease. However, mutation rates are not uniform across each eukaryotic genome. Rates for insertion/deletion (indel) mutations have been found to vary widely when examined in vitro and at specific loci in vivo. Here, we report the genome-wide rates of formation and repair of indels made during replication of yeast nuclear DNA. Using over 6000 indels accumulated in four mismatch repair (MMR) defective strains, and statistical corrections for false negatives, we find that indel rates increase by 100 000-fold with increasing homonucleotide run length, representing the greatest effect on replication fidelity of any known genomic parameter. Nonetheless, long genomic homopolymer runs are overrepresented relative to random chance, implying positive selection. Proofreading defects in the replicative polymerases selectively increase indel rates in short repetitive tracts, likely reflecting the distance over which Pols δ and ϵ interact with duplex DNA upstream of the polymerase active site. In contrast, MMR defects hugely increase indel mutagenesis in long repetitive sequences. Because repetitive sequences are not uniformly distributed among genomic functional elements, the quantitatively different consequences on genome-wide repeat sequence instability conferred by defects in proofreading and MMR have important biological implications.