gebhart/patrepeval huggingface dataset:
Enantioselective Conjugate Addition of Organomagnesium Amides to Enamidomalonates:  Synthesis of Either Enantiomer of β-Amino Acid Derivatives (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTEnantioselective Conjugate Addition of Organomagnesium Amides to Enamidomalonates: Synthesis of Either Enantiomer of β-Amino Acid DerivativesMukund P. Sibi and Yasutomi AsanoView Author Information Department of Chemistry North Dakota State University Fargo, North Dakota 58105-5516 Cite this: J. Am. Chem. Soc. 2001, 123, 39, 9708–9709Publication Date (Web):September 7, 2001Publication History Received25 June 2001Revised17 August 2001Published online7 September 2001Published inissue 1 October 2001https://pubs.acs.org/doi/10.1021/ja016492chttps://doi.org/10.1021/ja016492crapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views1192Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Conjugate acid-base pairs,Salts,Selectivity,Stereochemistry,Substitution reactions Get e-Alerts
Improving the performance of rosetta using multiple sequence alignment information and global measures of hydrophobic core formation (2001)

This study explores the use of multiple sequence alignment (MSA) information and global measures of hydrophobic core formation for improving the Rosetta ab initio protein structure prediction method. The most effective use of the MSA information is achieved by carrying out independent folding simulations for a subset of the homologous sequences in the MSA and then identifying the free energy minima common to all folded sequences via simultaneous clustering of the independent folding runs. Global measures of hydrophobic core formation, using ellipsoidal rather than spherical representations of the hydrophobic core, are found to be useful in removing non-native conformations before cluster analysis. Through this combination of MSA information and global measures of protein core formation, we significantly increase the performance of Rosetta on a challenging test set. Proteins 2001;43:1-11.
Biocatalysis as a green route for recycling the recalcitrant plastic polyethylene terephthalate (2017)

Biocatalysis can enable a closed-loop recycling of post-consumer PET waste.
A new Reed-Solomon code decoding algorithm based on Newton's interpolation (1993)

A Reed-Solomon code decoding algorithm based on Newton's interpolation is presented. This algorithm has as main application fast generalized-minimum-distance decoding of Reed-Solomon codes. It uses a modified Berlekamp-Massey algorithm to perform all necessary generalized-minimum-distance decoding steps in only one run. With a time-domain form of the new decoder the overall asymptotic generalized-minimum-distance decoding complexity becomes O(dn), with n the length and d the distance of the code (including the calculation of all error locations and values). This asymptotic complexity is optimal. Other applications are the possibility of fast decoding of Reed-Solomon codes with adaptive redundancy and a general parallel decoding algorithm with zero delay.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
DGT, a novel heterocyclic diterpenoid, effectively suppresses psoriasis via inhibition of STAT3 phosphorylation (2020)

DGT has great potential as a novel therapeutic agent for the treatment of psoriatic skin disease.
High-Speed Gas Chromatographic Analysis of a Simulated Process Stream using On-Column Thermal Desorption Modulation for Sample Preconcentration and Introduction (1990)

Journal Article High-Speed Gas Chromatographic Analysis of a Simulated Process Stream using On-Column Thermal Desorption Modulation for Sample Preconcentration and Introduction Get access Zaiyou Liu, Zaiyou Liu Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois 62901-4409 Search for other works by this author on: Oxford Academic PubMed Google Scholar Minquan Zhang, Minquan Zhang Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois 62901-4409 Search for other works by this author on: Oxford Academic PubMed Google Scholar John B. Phillips John B. Phillips † Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, Illinois 62901-4409 †Author to whom correspondence should be addressed. Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Chromatographic Science, Volume 28, Issue 11, November 1990, Pages 567–571, https://doi.org/10.1093/chromsci/28.11.567 Published: 01 November 1990 Article history Received: 10 April 1989 Revision received: 26 March 1990 Published: 01 November 1990
An automatic intonation tone contour labelling and classification algorithm (2002)

Automatic recognition and classification of intonation contours in natural speech has the potential to improve the performance and useability of speech recognition systems, particularly those employed in automated speech response applications. The role of intonation in signalling meaning in natural English speech is well understood by linguists, and has been incorporated into the functional systemic grammar model of language. In this paper we describe the first stage of the development of a rule-based algorithm for intonation contour labelling and recognition which takes as its data input a stylised piecewise-linear approximation to the speech pitch contour. A key feature of the algorithm is the use of perceptually-based weighting of the segmented contour. Our results show good correspondence between the results of the automatic contour matching process and that of an expert human listener for a wide range of contours.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Digital image processing using MATLAB (2009)

1. Introduction. 2. Fundamentals. 3. Intensity Transformations and Spatial Filtering. 4. Frequency Domain Processing. 5. Image Restoration. 6. Color Image Processing. 7. Wavelets. 8. Image Compression. 9. Morphological Image Processing. 10. Image Segmentation. 11. Representation and Description. 12. Object Recognition.
Spatial Information Management Based on Web Services (2008)

It is bottleneck that WebGIS got spatial data,because of bandwidth of Internet. It is a puzzle for long time that how to meet with the exigent requirement of people. The new theory,method and model should be crea-ted. For unified manage and running speed for spatial data,this paper built a spatial database model and created a model of spatial Index. This model was satisfied for WebGIS. This model was built in the WebGIS.
Reverse Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibodies against SAG1-Related Sequence, SAG2A, and p97 Antigens from<i>Toxoplasma gondii</i>To Detect Specific Immunoglobulin G (IgG), IgM, and IgA Antibodies in Human Sera (2008)

The present study aimed to evaluate the performance of three monoclonal antibodies (MAbs) in reverse enzyme-linked immunosorbent assays (ELISAs) for detecting immunoglobulin G (IgG), IgM, and IgA antibodies against Toxoplasma gondii in 175 serum samples from patients at different stages of T. gondii infection, as defined by both serological and clinical criteria, as follows: recent (n = 45), transient (n = 40), and chronic (n = 55) infection as well as seronegative subjects (n = 35). The results were compared with those obtained by indirect ELISA using soluble Toxoplasma total antigen (STAg). Our data demonstrated that MAb A3A4 recognizes a conformational epitope in SAG1-related-sequence (SRS) antigens, while A4D12 and 1B8 recognize linear epitopes defined as SAG2A surface antigen and p97 cytoplasmatic antigen, respectively. Reverse ELISA for IgG with A3A4 or A4D12 MAbs was highly correlated with indirect ELISA for anti-STAg IgG, whereas only A4D12 reverse ELISA showed high correlation with indirect ELISA for IgM and IgA isotypes. To our knowledge, this is the first report analyzing the performance of a reverse ELISA for simultaneous detection of IgG, IgM, and IgA isotypes active toward native SAG2A, SRS, and p97 molecules from STAg, using a panel of human sera from patients with recent and chronic toxoplasmosis. Thus, reverse ELISA based on the capture of native SAG2A and SRS antigens of STAg by MAbs could be an additional approach for strengthening the helpfulness of serological tests assessing the stage of infection, particularly in combination with highly sensitive and specific assays that are frequently used nowadays for diagnosis of toxoplasmosis during pregnancy or congenital infection in newborns.
Strawberry Vein-Banding Virus, a Member of the Cauliflower Mosaic Virus Group (1973)

SUMMARY Cytoplasmic inclusions, made up of a dense, finely granular matrix up to 3 to 4 µm in diam. were consistently found in ultrathin sections of leaf tissues from Fragaria vesca infected with strawberry vein-banding virus (SVBV). Isometric particles, usually with ring-like profiles, 40 to 45 nm in diam. were seen either embedded in the dense matrix or scattered in some electron-lucent areas of the inclusion. The particle morphology and the type of the inclusion found associated with SVBV places it in the cauliflower mosaic virus group.
Studies on the ocular pharmacology of prostaglandin D2. (1990)

Prostaglandin D2 (PGD2) exerts a variety of biologic actions in the eye; these include ocular hypotension and inflammatory effects on the conjunctiva. The profile of activity of PGD2 in ocular tissues was compared to that of BW 245C, a selective agonist for the PGD2-sensitive (DP) receptor, and to that of the biologically active metabolites of PGD2, 9 alpha,11 beta-prostaglandin F2 (9 alpha,11 beta-PGF2) and prostaglandin J2 (PGJ2). PGD2 produced a dose-dependent decrease in intraocular pressure and in the conjunctiva it caused increased conjunctival microvascular permeability, eosinophil infiltration and goblet cell depletion. Although BW 245C was equipotent to PGD2 as an ocular hypotensive agent, it did not cause pathological effects in the conjunctiva. Thus, the ocular hypotensive effect of PGD2 may be separated from inflammatory effects on the conjunctiva by employing a selective DP-receptor agonist such as BW 245C. 9 alpha,11 beta-PGF2 was a weak ocular hypotensive and did not cause conjunctival inflammation. PGJ2 produced no significant effect on intraocular pressure. PGJ2 did not elicit a microvascular permeability response in the conjunctiva, but was inflammatory in other respects and caused eosinophil infiltration and goblet cell depletion similar to PGD2. Thus, both the ocular hypotensive actions and the conjunctival pathology of PGD2 may be replicated individually by employing PGD2 analogues and metabolites.
DNA binding strength increases the processivity and activity of a Y-Family DNA polymerase (2017)

DNA polymerase (pol) processivity, i.e., the bases a polymerase extends before falling off the DNA, and activity are important for copying difficult DNA sequences, including simple repeats. Y-family pols would be appealing for copying difficult DNA and incorporating non-natural dNTPs, due to their low fidelity and loose active site, but are limited by poor processivity and activity. In this study, the binding between Dbh and DNA was investigated to better understand how to rationally design enhanced processivity in a Y-family pol. Guided by structural simulation, a fused pol Sdbh with non-specific dsDNA binding protein Sso7d in the N-terminus was designed. This modification increased in vitro processivity 4-fold as compared to the wild-type Dbh. Additionally, bioinformatics was used to identify amino acid mutations that would increase stabilization of Dbh bound to DNA. The variant SdbhM76I further improved the processivity of Dbh by 10 fold. The variant SdbhKSKIP241-245RVRKS showed higher activity than Dbh on the incorporation of dCTP (correct) and dATP (incorrect) opposite the G (normal) or 8-oxoG(damaged) template base. These results demonstrate the capability to rationally design increases in pol processivity and catalytic efficiency through computational DNA binding predictions and the addition of non-specific DNA binding domains.
What it Means to Be a Market-Oriented Newspaper (1998)

Survey results find that traditional journalistic values remain important at market-oriented newspapers, though senior editors tend to report more interaction with departments outside the newsroom – including the advertising department.
Outbreak of Sternal Surgical Site Infections Due to<i>Pseudomonas aeruginosa</i>Traced to a Scrub Nurse with Onychomycosis (2001)

From 19 February 1999 through 31 October 1999, 16 (8.6%) of 185 patients who underwent median sternotomy developed infections with Pseudomonas aeruginosa. Seven patients had mediastinitis, 5 had deep sternal wound infection, 2 had superficial sternal wound infection, 1 had prosthetic valve endocarditis, and 1 had sepsis. Pulsed-field gel electrophoresis confirmed that all 13 isolates that were available for typing were the same strain. Cultures of hand specimens identified 1 nurse from whom the same strain of P. aeruginosa was repeatedly isolated; the nurse had been in contact with all 16 infected patients. Investigation revealed that the nurse had severe onycholysis and onychomycosis of the right thumbnail. Cultures of samples of this nail's subungual region and of multiple cosmetic products from the nurse's home yielded the identical P. aeruginosa strain. This outbreak of surgical site infections due to P. aeruginosa was caused by wound contamination from the thumbnail of this nurse, despite her appropriate use of latex surgical gloves.
Noise and flow structure of a tone-excited jet (1982)

Detailed and well-controlled acoustic as well as turbulence measurements are made of a tone-excited jet to obtain an understanding of the mechanism of broadband noise augmentation. Results are presented for a range of excitation frequencies and levels both with and without flight simulation. Results of measurements of the large-scale instability waves within the excited jet are also described. Changes in the radiated noise and the flow structure and their interrelationship are then discussed.
Sea Water Attenuation Measurements with a Ruby Laser (1963)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text J. P. Mutschlecner, D. K. Burge, and E. Regelson, "Sea Water Attenuation Measurements with a Ruby Laser," Appl. Opt. 2, 1202-1203 (1963) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Elliptic curve parameters generation (2004)

In this paper a method of elliptic curves (EC) parameters generating has been considered. The proposed method is based on the usage of Jacobi symbol and square remains. On the base of this method algorithms as well as software, which allow to effectively generate the EC coefficient have been developed. In the paper, the results of EC parameters generating have been presented.
Thymidylate Synthase Activity from <i>Chlamydomonas</i> Cells and Cultured Tissues of <i>Nicotiana, Pinus</i>, and <i>Daucus</i> (1979)

Prior preparation of N(5),N(10)-methylenetetrahydrofolate from dl-tetrahydrofolate and [(14)C]formaldehyde resulted in an improved assay for thymidylate synthase. Although preparations from tobacco seedlings and cotton root tips (0.25 centimeter) were inconsistent with respect to enzyme activity, extracts from actively growing cell cultures of Chlamydomonas, Nicotiana hybrid callus, Pinus callus, and Daucus proembryonic cells contained significant levels of thymidylate synthase (12.3 to 23.8 nanomoles of thymidylate formed per milligram of protein per hour).
Development of 1.02-µm pump laser diodes (1996)

New 1.02-μm strained InGaAs pump laser diodes exhibit high coupling efficiency of above 65 % for laser-to-single mode fiber coupling with a single aspheric lens. A small vertical divergence angle of 17 degrees and a small divergence aspect ratio of 1.3 were obtained by using a new layer structure with low refractive index layers. A preliminary aging test reveals that they are as reliable as conventional strained InGaAs pump lasers. They also demonstrated a high single mode fiber coupling power of above 300 mW.
Allosteric characteristics of GTP cyclohydrolase I from <i>Escherichia coli</i> (1992)

The kinetic and regulatory properties of GTP cyclohydrolase I were investigated using an improved enzyme assay and direct determination of the product, dihydroneopterin triphosphate. The enzyme was purified from Escherichia coli to absolute homogeneity as demonstrated by N-terminal sequencing of up to 50 amino acid residues. A 30-residue internal fragment showed 42% similarity with rat liver GTP cyclohydrolase I. The enzyme did not obey Michaelis-Menten kinetics or show a sigmoid reaction curve. The substrate saturation kinetics were found to be slow with low response to minor changes in GTP concentrations. GTP cyclohydrolase I has a relatively high apparent Km. The values are slightly different for enzyme purified by GTP-agarose (100 microM) and UTP-agarose (110 microM). Low turnover numbers of 12/min and 19/min were calculated for the respective enzyme preparations. GTP-cyclohydrolase-I activity was modulated in Vmax by K+, divalent cations, UTP and tetrahydrobiopterin. Divalent cations, such as Mg2+, had an activating effect with an optimum at 8 mM Mg2+. A different catalytic function and formation of a new, unidentified product by GTP cyclohydrolase I was observed in the presence of Ca2+. In the presence of 1 mM EDTA and Mg2+, GTP-cyclohydrolase-I activity was strongly inhibited by chelate complexes. UTP proved not to be a competitive inhibitor, but a positive modulator. The inhibition by chelate complexes was totally abolished by UTP. Tetrahydrobiopterin showed an inhibitory effect, with 50% inhibition at 100 microM tetrahydrobiopterin. UTP was able to reduce the inhibition by tetrahydrobiopterin. Using monoclonal antibody 1F11 (related to the GTP-binding site), and monoclonal antibody NS7 (mimicking tetrahydrobiopterin), different binding sites were demonstrated for GTP and tetrahydrobiopterin on each enzyme subunit. Western-blot competition analysis revealed a UTP-binding site different from the binding sites of GTP and tetrahydrobiopterin. Based on the kinetic behaviour and the kind of modulations observed we defined GTP cyclohydrolase I as an M-class allosteric enzyme.
Characterization of internalization and endosome formation of epidermal growth factor in transfected NIH-3T3 cells by computerized image-intensified three-dimensional fluorescence microscopy. (1989)

Computerized image-intensified fluorescence microscopy has been used to quantify routing and subcellular concentrations of rhodaminated EGF (Rh-EGF) during its receptor-mediated endocytosis in two transfected NIH-3T3 cell lines expressing 2 X 10(5) and 1.5 X 10(6) receptors per cell, respectively. A series of images were digitized by focusing at different depths through the volume of a single cell. The digitized pictures were corrected for fluorescence photobleaching, and removal of out-of-focus fluorescence contributions by deconvolution using the point spread function of the microscope optics (Agard, D. A., and J. W. Sedat. 1980. Proc. Soc. Photo-Opt. Instr. Eng. 264:110-117) allowed automatic computer analysis of the time dependence of endosomal vesicle size and fluorescence intensity in a live cell and also enabled the study of isolated vesicles. An increase in the amount of fluorescence bound to the cell surface, either by increasing the number of receptors expressed per cell or the concentration of Rh-EGF in the incubation drop, yielded an increase in the total fluorescence of internalized vesicles without an increase in their number and area. The linear relation between fluorescence intensity and area for vesicles at different times indicates that EGF concentration is conserved. This is compatible with fusion of small vesicles to form larger ones. However, as endocytosis proceeds, a twofold increase in the slope of the fluorescence vs. area plots is observed for larger vesicles, suggesting that active sorting causes the EGF to be concentrated. Alternatively, this factor could be produced by cumulative fluorescence contributions from stacked membranes. Since coated pits are internalized independent of their occupancy with EGF receptor, we propose that endocytosis does not involve a mechanism specifically recognizing occupied receptor but is rather triggered by a global intracellular event.
Regulated Expression and RNA Processing of Transcripts from the <i>Srp20</i> Splicing Factor Gene during the Cell Cycle (1997)

Eukaryotic splicing factors belonging to the SR family are essential splicing factors consisting of an N-terminal RNA-binding region and a C-terminal RS domain. They are believed to be involved in alternative splicing of numerous transcripts because their expression levels can influence splice site selection. We have characterized the structure and transcriptional regulation of the gene for the smallest member of the SR family, SRp20 (previously called X16). The mouse gene encoding SRp20, termed Srp20, consists of one alternative exon and six constitutive exons and was mapped to a 2-centimorgan interval on chromosome 17. When cells are transfected with SRp20 genomic DNA, both standard and alternatively spliced transcripts and corresponding proteins are produced. Interestingly, in starved (G0) cells, the amount of SRp20 mRNA containing the alternative exon is large, whereas the amount of the standard SRp20 mRNA without the alternative exon is small. When starved cells are stimulated with serum, the alternative form is lost and the standard form is induced. These results suggest that splicing could be regulated during the cell cycle and that this could be, at least in part, due to regulated expression of SR proteins. Consistent with this, experiments with synchronized cells showed an induction of SRp20 transcripts in late G1 or early S. We have also characterized the promoter of SRp20. It lies within a GC-rich CpG island and contains two consensus binding sites for E2F, a transcription factor thought to be involved in regulating the cell cycle. These motifs may be functional since reporter constructs with the SRp20 promoter can be stimulated by cotransfection with E2F expression plasmids.
PPP Predictor Compression Protocol (1996)

The Point-to-Point Protocol (PPP) [1] provides a standard method of encapsulating multiple protocol datagrams over point-to-point links.