gebhart/patrepeval huggingface dataset:
Comparison of the Effects of Growth Factors on Retroviral Vector-Mediated Gene Transfer and the Proliferative Status of Human Hematopoietic Progenitor Cells (1990)

We have examined the ability of the recombinant hematopoietic growth factors (HGF) interleukin-3 (IL-3), IL-6, and granulocyte-macrophage colony-stimulating factor (GM-CSF) to increase retroviral vector-mediated gene transfer into human hematopoietic progenitor cells (HPC). The efficiency of neo gene transfer by the N2 vector into human HPC was enhanced by preculture with either GM-CSF or IL-3 (but not IL-6) and with each combination of the three factors. The combination of IL-3 plus IL-6 consistently produced significantly higher levels of G418-resistant colonies (50-60%) than any of the other combinations of HGF tested. Following preculture with HGF and transduction by N2, marrow was maintained in long-term bone marrow culture (LTBMC) for 2 months. The levels of G418-resistant HPC remained stable, and no apparent depletion of total HPC content resulted from the prior exposure to highly stimulatory doses of factors. The proliferative status of the HPC, following exposure to the HGF, was measured as the percentage of HPC that were inhibited from forming colonies by exposure to the S-phase-specific drug, hydroxyurea. The ability of the different HGF to increase the rate of gene transfer by N2 correlated significantly with the extent to which they stimulated HPC proliferation. These results suggest that the mechanism by which HGF increase rates of gene transfer into HPC is by stimulating cell proliferation. Techniques that produce high rates of gene transfer into long-lived human HPC will facilitate studies to quantitate expression of exogenous genes in hematopoietic cells and may be applicable to clinical gene therapy.
Overview of Materials and Power Applications of Coated Conductors Project (2011)

There are high expectations for coated conductors in electric power applications such as superconducting magnetic energy storage (SMES) systems, power cables, and transformers owing to their ability to contribute to stabilizing and increasing the capacity of the electric power supply grid as well as to reducing CO 2 emission as a result of their high critical-current characteristics. Research and development has been performed on wires/tapes and electric power devices worldwide. The Materials and Power Applications of Coated Conductors (M-PACC) Project is a five-year national project in Japan started in 2008, supported by the Ministry of Economy, Trade and Industry (METI) and the New Energy and Industrial Technology Development Organization (NEDO), to develop both coated conductors that meet market requirements and basic technologies for the above-mentioned power applications using coated conductors. In this article, research and development results are reviewed and compared with the interim/final targets of the project, and future prospects are discussed.
Programme evaluation : can it improve human resource management practice? : original research (2012)

Orientation : This is the final article in the special edition on human resource (HR) programmes and evaluation. Its starting point is that programme evaluation is the application of a wide range of social science research methods that provide credible information about the need, use, planning, effectiveness and cost of a programme.
Research purpose : The purpose of this article is to review the other articles in this volume, and to draw out general conclusions about their contributions to knowledge in the field.
Motivation for the study : If evaluations are undertaken in the HR domain in South Africa, they remain mostly unpublished, and thus cannot contribute to a knowledge base for the field.
Research design, approach and method : This article provides a theory-based approach to programme evaluation. The seven articles were analysed in terms of two major functions of programme evaluation, namely to ask 'How does a programme work?', and 'Does it work?'
Main findings : Eight overarching themes are identified in the articles included in this volume.
Practical/managerial implications : The evidence discussed here can be used to make better decisions, promote organisational learning, improve practice, and enhance employee wellbeing.
Contribution/value-add : The main contribution of this concluding article is its argument that research and theory in this field can enhance the work of HR professionals, by providing evidence about how 'good' a programme is, and why it is good. This adds substantial value in a world characterised by accountability and evidence-based practice.
Synthesis and Structural Characterization of the Tetrakis(methimazolyl)borates Li[Bmt<sub>4</sub>] and [Ru(<i>p</i>‐cymene)(Bmt<sub>4</sub>)][PF<sub>6</sub>] (2009)

Abstract The lithium tetrakis(methimazolyl)borate, Li[Bmt 4 ], is accessible from the reaction of Li[BH 4 ] with four equivalents of methimazole. The crystal structure of Li[Bmt 4 ] supported by four water molecules is described. Reaction of Li[Bmt 4 ] with [Ru( p ‐cymene)Cl 2 ] 2 and subsequent treatment with NH 4 PF 6 in CH 3 CN results in the formation of the complex [Ru( p ‐cymene)(Bmt 4 )][PF 6 ]. In addition, we report the result of the X‐ray structure analysis of the chiral Ru complex [Ru( p ‐cymene)(Bmt 4 )][PF 6 ].
Intracoronary ultrasound catheter motion compensation using the generalized Hough transform (2002)

Three-dimensional renderings of a coronary artery can be constructed by stacking a series of contiguous two-dimensional intracoronary ultrasound (ICUS) images obtained during pullback of the transducer. However, visualization and further image processing of the 3-D image are hampered by cardiac cycle-dependent catheter motion. The generalized Hough transform (GHT) was used to automatically propagate medial-adventitial boundary estimates through a sequence of 30 ICUS images. The boundary centroids were then translated to the image center to remove the catheter motion artifact. This automated tracking strategy was compared to previously described boundary defection methods applied to each image in the sequence individually. The results show that the generalized Hough transform can be used to remove or reduce catheter motion artifacts in ICUS image sequences.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Proteolysis and membrane capture of F-spondin generates combinatorial guidance cues from a single molecule (2007)

The formation of neuronal networks is governed by a limited number of guidance molecules, yet it is immensely complex. The complexity of guidance cues is augmented by posttranslational modification of guidance molecules and their receptors. We report here that cleavage of the floor plate guidance molecule F-spondin generates two functionally opposing fragments: a short-range repellent protein deposited in the membrane of floor plate cells and an adhesive protein that accumulates at the basement membrane. Their coordinated activity, acting respectively as a short-range repellant and a permissive short-range attractant, constricts commissural axons to the basement membrane beneath the floor plate cells. We further demonstrate that the repulsive activity of the inhibitory fragment of F-spondin requires its presentation by the lipoprotein receptor-related protein (LRP) receptors apolipoprotein E receptor 2, LRP2/megalin, and LRP4, which are expressed in the floor plate. Thus, proteolysis and membrane interaction coordinate combinatorial guidance signaling originating from a single guidance cue.
Basins of attraction in driven dynamical systems (1989)

The organization of multiple coexisting basins of attraction in two-dimensional driven dynamical systems is studied. This study is carried out, in particular, for the laser with modulated parameter and the H\'enon map. Basin organization is governed primarily by the ordering of heteroclinic and homoclinic connections of regular saddles. This organization is complex yet systematic, with accumulation structures governed by the order of saddle connections. We study the evolution of a basin in the presence of multiple coexisting basins, from (before) birth to (beyond) death. Many of the processes that occur are canonical. These include the reorganization of existing basins in preparation for the creation of a new basin by saddle-node bifurcation, the increased intertwining, in Poincar\'e section, of the components of a basin, and the increased wrinkling of each component as the basin evolves from birth to death. These also include the processes that mark disintegration and the ultimate demise of the basin, beginning at homoclinic tangency and terminating in its disappearance or enlargement in a crisis.
Stuck-at tuple-detection: a fault model based on stuck-at faults for improved defect coverage (2002)

N-detection stuck-at test sets were shown to be effective in achieving high defect coverages for benchmark circuits. However, the definition of n-detection rest sets allows the same set of faults to be detected by several different tests, thus potentially detecting the same defects. We propose an extension of the n-detection model that alleviates this problem by considering m-tuples of faults and requiring that different tests would detect different m-tuples. We present experimental results to support this model.
Approaches to High-Throughput Physical Form Screening and Discovery (2018)

Alastair J. Florence Solid-State Research Group, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, U.K.INTRODUCTION The development of experimental methods for increasing the throughput and effi - ciency of all stages of drug discovery and development is an important area of research within the pharmaceutical industry. In the context of physical form discovery of pharmaceuticals, the fundamental aim when establishing a rigorous experimental crystallization search strategy is to achieve as wide a coverage of crystallization conditions and methods as possible within the constraints of available time, material, and resources. For the purposes of this chapter, physical form will be taken to mean any solid form of the compound being examined, whether crystalline (polymorphs, solvates, salts, and co-crystals) or non-crystalline (amorphous). Automated crystallization approaches allow the basic process steps involved in a typical manual solution recrystallization method ( Fig. 1 ) to be effi ciently replicated over large numbers of parallel experiments. In principle, this enables the routine implementation of search strategies designed to maximize the diversity of crystallization conditions tested, allowing the experimental search to be carried out over a fi ner grid (e.g., a larger solvent library).
Frustrated Lewis pairs incorporating the bifunctional Lewis acid 1,1′-fc{B(C<sub>6</sub>F<sub>5</sub>)<sub>2</sub>}<sub>2</sub>: reactivity towards small molecules (2017)

Applications of the bifunctional ferrocenediyl Lewis acid 1,1'-fc{B(C<sub>6</sub>F<sub>5</sub>)<sub>2</sub>}<sub>2</sub> in frustrated Lewis pair (FLP) chemistry are described. The coordination (or otherwise) of a range of sterically encumbered C-, N- and P-centred Lewis bases has been investigated, with lutidine, tetramethylpiperidine, PPh<sub>3</sub>, P<sup>t</sup>Bu<sub>3</sub> and the expanded ring carbene 6Dipp being found to be sterically incapable of coordinate bond formation. The chemistry of a range of these FLPs in the presence of H<sub>2</sub>O, NH<sub>3</sub>, CO<sub>2</sub> and cyclohexylisocyanate (CyNCO) has been investigated, with the patterns of reactivity identified including simple coordination chemistry, E-H bond cleavage and C-B insertion.
CuInS2 thin-film homojunction solar cells (1977)

The fabrication and characteristics of n,p-CuInS2 thin-film homojunction solar cells are presented. Dark and light I-V characteristics are reported for a 0.124-cm2 device with η=3.33%, Voc=0.41 V, Isc=2.34 mA, and FF=0.43. A best efficiency of 3.62% is cited. An examination of the forward dark lnJ-vs-V characteristics indicates that the recombination-generation mechanism at the junction dominates the device operation. The spectral characteristics are presented and indicate a maximum near the wavelength (λ=0.8 μ) corresponding to the energy gap of CuInS2, with a relatively low quantum efficiency (&lt;0.45). The stability and limitations of this photovoltaic device are discussed.
Sequence analysis of the GP, NP, VP40 and VP24 genes of Ebola virus isolated from deceased, surviving and asymptomatically infected individuals during the 1996 outbreak in Gabon: comparative studies and phylogenetic characterization (2002)

The aims of this study were to determine if the clinical outcome of Ebola virus (EBOV) infection is associated with virus genetic structure and to document the genetic changes in the Gabon strains of EBOV by sequencing the GP, NP, VP40 and VP24 genes from deceased and surviving symptomatic and asymptomatic individuals. GP and NP sequences were identical in the three groups of patients and only one silent substitution occurred in the VP40 and VP24 genes in asymptomatic individuals. A strain from an asymptomatic individual had a reverse substitution to the Gabon-94 sequence, indicating that minor virus variants may cocirculate during an outbreak. These results suggest that the different clinical outcomes of EBOV infection do not result from virus mutations. Phylogenetic analysis confirmed that Gabon-96 belonged to the Zaire subtype of EBOV and revealed that synonymous substitution rates were higher than nonsynonymous substitution rates in the GP, VP40 and VP24 genes. In contrast, nonsynonymous substitutions predominated over synonymous substitutions in the NP gene of the two Gabon strains, pointing to divergent evolution of these strains and to selective pressures on this gene.
Exploring partitioning methods for 3D Networks-on-Chip utilizing adaptive routing model (2011)

Three-Dimensional (3D) integration is a solution to the interconnect bottleneck in Two-Dimensional (2D) MultiProcessor System on Chip (MPSoC). 3D IC design improves performance and decreases power consumption by replacing long horizontal interconnects with shorter vertical ones. As the multicast communication is utilized commonly in various parallel applications, the performance can be significantly improved by supporting of multicast operations at the hardware level. In this paper, we propose a set of partitioning approaches each with a different level of efficiency. In addition, we present an advantageous method named Recursive Partitioning (RP) in which the network is recursively partitioned until all partitions contain comparable number of nodes. By this approach, the multicast traffic is distributed among several subsets and the network latency is considerably decreased. We also present Minimal Adaptive Routing (MAR) algorithm for the unicast and multicast traffic in 3D-mesh Networks-on-Chip (NoCs). The idea behind the MAR algorithm is utilizing the Hamiltonian path to provide a set of alternative paths.
Test fonctionnel des circuits intégrés digitaux (1985)

L'objet de cette thèse est l'étude de deux méthodes de génération automatique de vecteurs de test pour les circuits intégrés digitaux. Après un rappel des problèmes actuels posés par le test des circuits VLSI (partie I), deux méthodes de génération automatique de vecteurs de test adressant deux types différents d'hypothèses de pannes sont présentées: une méthode heuristique de génération de vecteurs (partie II) et une méthode de test pseudo-exhaustif (partie III)
Selective reduction of proadrenocorticotropin/endorphin proteins and messenger ribonucleic acid activity in mouse pituitary tumor cells by glucocorticoids (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelective reduction of proadrenocorticotropin/endorphin proteins and messenger ribonucleic acid activity in mouse pituitary tumor cells by glucocorticoidsJames L. Roberts, Marcia L. Budarf, John D. Baxter, and Edward HerbertCite this: Biochemistry 1979, 18, 22, 4907–4915Publication Date (Print):October 1, 1979Publication History Published online1 May 2002Published inissue 1 October 1979https://pubs.acs.org/doi/10.1021/bi00589a019https://doi.org/10.1021/bi00589a019research-articleACS PublicationsRequest reuse permissionsArticle Views26Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Zinc through the Three Domains of Life (2006)

Zinc is one of the metal ions essential for life, as it is required for the proper functioning of a large number of proteins. Despite its importance, the annotation of zinc-binding proteins in gene banks or protein domain databases still has significant room for improvement. In the present work, we compiled a list of known zinc-binding protein domains and of known zinc-binding sequence motifs (zinc-binding patterns), and then used them jointly to analyze the proteome of 57 different organisms to obtain an overview of zinc usage by archaeal, bacterial, and eukaryotic organisms. Zinc-binding proteins are an abundant fraction of these proteomes, ranging between 4% and 10%. The number of zinc-binding proteins correlates linearly with the total number of proteins encoded by the genome of an organism, but the proportionality constant of Eukaryota (8.8%) is significantly higher than that observed in Bacteria and Archaea (from 5% to 6%). Most of this enrichment is due to the larger portfolio of regulatory proteins in Eukaryota.
Synthetic peptides derived from an N‐terminal domain of the E2 protein of GB virus C in the study of GBV‐C/HIV‐1 co‐infection (2012)

Synthetic peptides derived from GB virus C (GBV-C) have previously been studied in our group for the development of new systems capable of diagnosing diseases caused by this humanotropic virus. We also recently described specific peptide domains of the E2 envelop protein of GBV-C that have the capacity to interfere with the HIV-1 fusion peptide, produce a notable decrease in cellular membrane fusion, and perturb HIV-1 infectivity in a dose-dependent manner. The present work discloses the design and synthesis of both linear and cyclic branched peptides based on a previously reported N-terminal sequence of the GBV-C E2 protein. Immunoassays and cell-cell fusion assays were performed to evaluate their diagnostic value to detect anti-GBV-C antibodies in HIV-1 patients, as well as their putative anti-HIV-1 activity as entry inhibitors. Our results showed that chemical modifications of the selected E2(7-26) linear peptide to afford cyclic architecture do not result in an enhanced inhibition of gp41 HIV-1-mediated cell-cell fusion nor improved sensitivity in the detection of GBV-C antibodies in HIV-1 co-infected patients. Thus, the ELISA data reinforce the potential utility of linear versions of the E2(7-26) region for the development of new peptide-based immunosensor devices for the detection of anti-GBV-C antibodies in HIV-1 co-infected patients.
Riboprinting: A Tool for the Study of Genetic Diversity in Microorganisms<sup>1</sup> (1997)

Classical morphology-based methods of taxonomic and phylogenetic analysis are inadequate in many groups of structurally simple eukaryotes. Molecular methods can generate data independently of the complexity of the organisms' morphology. Riboprinting is one such technique, and involves restriction enzyme analysis of polymerase chain reaction amplified small subunit ribosomal RNA genes. The utility of the method is illustrated with examples from several genera of intestinal and bloodstream parasites. Among the applications of riboprinting are the detection of cryptic genetic variation within species, organism misidentifications and culture mix-ups, independent verification of DNA sequences, and the rapid generation of data useful in phylogenetic analyses.
84 dB 5.2 mA digitally-controlled variable gain amplifier (2008)

A digitally-controlled topology that reduces power dissipation to half that of the conventional one while occupying smaller die area and obtaining higher linearity is presented. In the 0.13 µm CMOS process, the VGA occupies an active area of 0.46 mm2 and dissipates an average current of 5.2 mA at 1.5 V. The gain-variation range is −32 to 52 dB with a gain error of less than ±1 dB. The IIP3, P1dB, NF at 52 dB of gain, and 3 dB bandwidth are −37 to +13 dBm, −42 to −2 dBm, 6.2 dB and 200 MHz, respectively.
Effect of the Sialyl Lewis X (SLe) Moiety on Splenic Accumulation of SLe<sup>x</sup>‐Carboxymethylpullulan Conjugate (2000)

Sialyl Lewis X (SLe(x)), an E-selectin ligand, was conjugated with carboxymethylpullulan (CMPul) and the disposition characteristics of this conjugate after intravenous administration were investigated using mice with ear edema. The concentration of 3H-labeled SLe(x)-CMPul in the spleen was significantly high. When CMPul was modified with a saccharide unable to bind to E-selectin, this splenic accumulation was not observed. The uptake of radiolabeled SLe(x)-CMPul by the spleen was completely inhibited by a 100-fold molar of cold SLe(x)-CMPul but not by a sialyl N-acetyllactosamine-CMPul conjugate (SLN-CMPul). Microautoradiography analyses revealed that SLe(x)-CMPul accumulated in the marginal zone of the spleen.
Integration of vision based image processing for multi-axis CNC machine tool safe and efficient trajectory generation and collision avoidance (2011)

International audience
Friedel-Crafts Alkylation of Substituted Benzenes by Allyldichlorosilane (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFriedel-Crafts Alkylation of Substituted Benzenes by AllyldichlorosilaneBong Woo Lee, Bok Ryul Yoo, Sun-Il Kim, and Il Nam JungCite this: Organometallics 1994, 13, 4, 1312–1316Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April 1994https://pubs.acs.org/doi/10.1021/om00016a038https://doi.org/10.1021/om00016a038research-articleACS PublicationsRequest reuse permissionsArticle Views397Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts