gebhart/patrepeval huggingface dataset:
Mycobacterial Phenolic Glycolipids with a Simplified Lipid Aglycone Modulate Cytokine Levels through Toll‐Like Receptor 2 (2013)

Phenolic glycolipids (PGLs) are virulence factors present in the cell walls of many pathogenic mycobacteria. PGLs have been implicated in various aspects of mycobacterial disease, but there are limited structure-activity data available for these molecules. We report here the preparation of seven synthetic PGL analogues, differing from the native compounds in the replacement of the complex phenolic lipid moiety with a p-methoxyphenyl group. The ability of these compounds to stimulate or inhibit the production of cytokines (TNF-α, IL-1β, IL-6, MCP-1) and nitric oxide (NO) was then evaluated by ELISA-based assays. None of the compounds stimulated the production of these biological signalling molecules. In contrast, they each displayed concentration-dependent inhibitory activity, related to the methylation pattern of the molecule and mediated by Toll-like receptor 2. Additional studies revealed that native PGL-I from Mycobacterium leprae and a synthetic PGL-I analogue containing a simplified lipid domain had enhanced inhibitory activities relative to the corresponding analogues containing the p-methoxyphenyl aglycone; however, the natural lipid phenolthiocerol was only weakly active. These studies reveal that synthetic molecules of this type can be used as probes for PGL function. Moreover, their ease of synthesis relative to the natural glycolipids, as well as their more favourable aqueous solubility, should allow for more thorough structure-activity relationship studies.
Static torque profiles of a hybrid stepper motor having relative eccentricity between stator and rotor axes (2003)

A smooth and controlled driving load for a hybrid stepper motor requires detailed knowledge of the motor’s static and detent torque profiles. Uncertainties to measured torque profiles may be due to rotor/stator concentric misalignment as one possible factor. This article presents the results of a detailed experimental analysis on a stepper motor’s relative eccentricity between the rotor and stator axes. The nonuniform airgap not only introduces a position-dependent fundamental detent torque along with the conventional fourth harmonic detent torque, but also changes the static torque profile. The sum effect on static torque is a changed torque equilibrium point, dependent both on rotor position and motor phase. Detent torque harmonic component analysis of a set of 0.5° hybrid stepper motors, with rotor-to-stator eccentricity as the control variable, showed how increased eccentricity exceeding a given limit will cause a loss of stable equilibrium of the detent torque at each step position.
TechMIS Newsletter (December 2001) (2001)

The December 2003 newsletter includes a special report on reauthorizing IDEA, a Washington Update, and a State Update.
Xanthine dehydrogenase and xanthine oxidase activity and gene expression in renal epithelial cells. Cytokine and steroid regulation. (1994)

Reactive oxygen species have been implicated in the tissue injury and loss of epithelial barrier function associated with a number of clinical disorders in which disregulated inflammation seems to be a dominant event, such as endotoxemia and viral syndromes. In these disorders, xanthine oxidase (XO) contained within the epithelial cell has been proposed as a major source of injurious reactive oxygen species. This study was undertaken in an effort to understand the regulation of xanthine dehydrogenase (XDH)/XO expression at both the activity and gene expression levels in the epithelial cell under conditions associated with the inflammatory response. The results indicate that TNF, IFN-gamma, IL-6, IL-1, and dexamethasone induce XDH/XO activity in bovine renal epithelial cells (MDBK). This pattern of XDH/XO regulation by cytokines and steroids is analogous to the profile of response seen by acute phase reactants. Metabolic labeling and immunoprecipitation revealed the increase in XDH/XO activity requires new protein synthesis. By Northern analysis, all cytokines and dexamethasone increased the level of the 5-kb XDH/XO mRNA. This increase was not detectable in the presence of actinomycin D but was further induced in the presence of cycloheximide, consistent with the major site of XDH/XO up-regulation occurring at the transcriptional level. XDH/XO mRNA was very stable, with no indication that the rates of transcript degradation contributed to differences in mRNA accumulation or ultimate activity levels. In addition to providing information on the regulation of XDH/XO, the data presented furthers the understanding of the epithelial cell's potential to actively respond to immunomodulators associated with injury/inflammation.
POSTERIOR VITREOUS DETACHMENT WITH MICROPLASMIN ALTERS THE RETINAL PENETRATION OF INTRAVITREAL BEVACIZUMAB (AVASTIN) IN RABBIT EYES (2010)

Increased BV retinal penetration is observed initially in eyes with an MP-induced PVD, and the mechanism is likely multifactorial. By 3 days, retinal penetration is similar in eyes with and without a PVD. Although it is difficult to directly extrapolate to humans, our study suggests that a PVD may alter the retinal penetration of BV.
AN IMPLANT RELEASING THE GONADOTROPIN HORMONE-RELEASING HORMONE AGONIST HISTRELIN MAINTAINS MEDICAL CASTRATION FOR UP TO 30 MONTHS IN METASTATIC PROSTATE CANCER (2000)

A histrelin implant suppresses LH and testosterone in prostate cancer for up to 30 months. This finding represents a significant improvement over existing preparations, which must be administered at 1 to 3-month intervals.
α-Lipoic acid stabilized DTX/IR780 micelles for photoacoustic/fluorescence imaging guided photothermal therapy/chemotherapy of breast cancer (2018)

Micellar nanoparticles have unique advantages as carriers for therapeutic or imaging agents, owing to their smaller size and better penetration of tumors. However, some agents, due to their physical or chemical properties, are difficult to load into micelles. IR780 is one of these agents, and is also a promising near-infrared dye for fluorescence imaging (FI)/photoacoustic imaging (PAI) and cancer photothermal therapy (PTT). Its hydrophobic and high crystallization structure results in limited bioavailability in vivo. It is difficult to load into micelles constructed from an amphiphilic block polymer with relatively low molecular weight. In this study, we use computer simulation and introduce another small biomolecule, α-lipoic acid, into the micelles constructed from a mPEG-PCL copolymer, to lower the energy of molecular interaction between MPEG-PCL and IR780, and expect to enhance the loading capacity of the micelles to IR780. The introduction of α-lipoic acid decreases the energy of molecular interaction between MEPG-PCL and IR780 from -46.18 kJ mol-1 to -196.52 kJ mol-1 and increases the loading capacity and stability of the mPEG-PCL micelles to IR780, which also maintains the loading capacity to DTX. We further construct DTX/IR780 co-loaded mPEG-PCL micelles for FI/PAI dual modal imaging guided PTT/chemotherapy of cancer. By FI and PAI evaluation in vitro and in vivo, we demonstrate that the DTX/IR780 co-loaded micelles can be used as FI and PAI probes. By further evaluating the therapeutic outcome of PTT/chemotherapy co-therapy of breast cancer, we demonstrate that the DTX/IR780 co-loaded mPEG-PCL micelles can serve as promising candidates for FI and PAI guided PTT/chemotherapy of breast cancer.
ChemInform Abstract: Structural Revisions of Blumenol C Glucoside and Byzantionoside B. (2010)

Abstract A modified Mosher′s method is used for the determination of the absolute stereochemistry of blumenol C glucoside (I) and byzantionoside B (II).
Sterically Crowded Gallium Amidinate Complexes (1999)

The sterically crowded gallium amidinate complexes {tBuC(NR‘)2}GaX2 (X = Cl, Me, Et, CH2Ph; R‘ = iPr, Cy, tBu) have been synthesized in good yield. X-ray crystallographic analyses show that the steric interactions between the tBu and R‘ groups influence the R‘−N−Ga angle and the steric environment at gallium.
Understanding mechanisms of vitiligo development in Smyth line of chickens by transcriptomic microarray analysis of evolving autoimmune lesions (2012)

The microarray results provided comprehensive information at the transcriptome level supporting the multifactorial etiology of vitiligo, where together with apparent inflammatory/innate immune activity and oxidative stress, the adaptive immune response plays a predominant role in melanocyte loss.
Single and Coupled Nanobeam Cavities (2013)

This book collects chapters on different theoretical and experimental aspects of photonics crystals for Nanophotonics applications. It is divided in two parts - a theoretical section and an experimental and applicative section. The first part includes chapters developing several numerical methods for analysis and design of photonic crystal devices, such as 2D ring resonators for filters, single and coupled nanobeam cavities, birefringence in photonic crystal cavities, threshold analysis in photonic crystal lasers, gap solitons in photonic crystals, novel photonic atolls, dynamic characteristics of photonic crystal filters. The second part focuses on some aspects of photonic crystals fabrication and relevant applications, such as nitrogen defect technology in diamond, silicon nitride free standing membranes, photonic crystals structures in silicon, photonic crystals for optical sensing.
High performance liquid chromatography with metal-solute complexes (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHigh performance liquid chromatography with metal-solute complexesFrancis K. Chow and Eli. GrushkaCite this: Anal. Chem. 1978, 50, 9, 1346–1353Publication Date (Print):August 1, 1978Publication History Published online1 May 2002Published inissue 1 August 1978https://pubs.acs.org/doi/10.1021/ac50031a039https://doi.org/10.1021/ac50031a039research-articleACS PublicationsRequest reuse permissionsArticle Views79Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Index Case for the Fungal Meningitis Outbreak in the United States (2012)

Persistent neutrophilic meningitis presents a diagnostic challenge, because the differential diagnosis is broad and includes atypical infectious causes. We describe a case of persistent neutrophilic meningitis due to Aspergillus fumigatus in an immunocompetent man who had no evidence of sinopulmonary or cutaneous disease. An epidural glucocorticoid injection was identified as a potential route of entry for this organism into the central nervous system, and the case was reported to the state health department.
The Role of a Single-stranded Nucleotide Loop in Transcriptional Regulation of the Human sod2 Gene (2007)

Manganese superoxide dismutase (MnSOD), a mitochondrial antioxidant enzyme, is necessary for survival of aerobic life. Previously, we demonstrated that a Sp1-based promoter is essential for constitutive transcription and a NF-kappaB-based intronic enhancer is responsible for cytokine-mediated induction. Here we show that nucleophosmin (NPM), a RNA-binding protein, binds to an 11G single-stranded loop in the promoter region and serves to integrate the Sp1 and NF-kappaB responses. Disruption of the loop structure causes a reduction of both constitutive and inductive transcription due to loss of the binding motif for NPM. Interaction of NF-kappaB.NPM.Sp1 facilitated by binding of NPM to the loop structure in the promoter region appears to comprise the basic complex for the transcriptional stimulation. These results suggest a novel molecular mechanism for communication between the enhancer and the GC-rich promoter.
No.4 ESS: The Switched Digital Network Plan (1977)

A plan for the introduction of time-division switching in the intertoll network is outlined. The plan uses the No. 4 ess as the switching vehicle. The plan defines a Switched Digital Network (sdn) which can evolve compatibly with the existing Switched Analog Network (san). The plan introduces 64 kilobits per second time-division multiplexed pcm as a standard switching signal format in the intertoll network. Facilities utilization, trunk design, timekeeping, and maintenance plans which are required for the new format are presented. Effects on intertoll network evolution, particularly voice performance, are assessed.
Molecular Mechanisms for the Induction of Peroxidase Activity of the Cytochrome <i>c</i>–Cardiolipin Complex (2011)

Induction of the peroxidase activity of cytochrome c (cyt c) by cardiolipin (CL) and H(2)O(2) in mitochondria is suggested to be a key event in early apoptosis. Although electrostatic interaction between the positively charged cyt c and negatively charged CL is a predominant force behind the formation of a specific cyt c-CL complex and sequential induction of the peroxidase activity, molecular mechanisms of hydrophobic interactions involving the fatty acyl chains of CL remain to be investigated. To elucidate the function of the acyl chains, particularly the role of the double bond, we synthesized a variety of CL analogues and examined their peroxidase inducing activity. Irrespective of the number of double bonds in the acyl chains, the peroxidase activity of cyt c induced by liposomes composed of 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) and a different CL (9:1 molar ratio) was similar, except for that of 1,1',2,2'-tetrastearoylcardiolipin (TSCL, C18:0)-containing liposomes. The peroxidase inducing activity of TSCL-containing liposomes was 3-4-fold greater than that of other CL-containing liposomes. The peroxidase activity induced by all CL-containing liposomes was much lower at high ionic strengths than that at low ionic strengths because of diminution of the electrostatic interaction. The peroxidase inducing effects of various CL-containing liposomes were related well to their ability to associate with cyt c. Thus, our results revealed that at low CL levels, the saturated acyl chain of CL is favorable for the activation of peroxidase activity of CL-bound cyt c and the proposed critical role of the double bond is not a general feature of the cyt c-CL interaction. The polarity of the membrane surface of TSCL-containing liposomes was slightly, but significantly, lower than that of other CL-containing liposomes, suggesting that the higher activating ability of TSCL-containing liposomes may be due to a reduced level of hydration of the polar head region reflecting tighter packing of the fully saturated acyl chains. Moreover, using CL analogues in which a central glycerol head moiety was modified, we revealed that the natural structure of the head moiety is not critical for the formation of the active cyt c-CL complex. The effects of the CL content of the liposomal membrane on the cyt c-CL interaction are discussed.
Green Sperm. Identification of Male Gamete Promoters in Arabidopsis (2005)

Previously, in an effort to better understand the male contribution to fertilization, we completed a maize (Zea mays) sperm expressed sequence tag project. Here, we used this resource to identify promoters that would direct gene expression in sperm cells. We used reverse transcription-polymerase chain reaction to identify probable sperm-specific transcripts in maize and then identified their best sequence matches in the Arabidopsis (Arabidopsis thaliana) genome. We tested five different Arabidopsis promoters for cell specificity, using an enhanced green fluorescent protein reporter gene. In pollen, the AtGEX1 (At5g55490) promoter is active in the sperm cells and not in the progenitor generative cell or in the vegetative cell, but it is also active in ovules, roots, and guard cells. The AtGEX2 (At5g49150) promoter is active only in the sperm cells and in the progenitor generative cell, but not in the vegetative cell or in other tissues. A third promoter, AtVEX1 (At5g62850) [corrected] was active in the vegetative cell during the later stages of pollen development; the other promoters tested (At1g66770 and At1g73350) did not function in pollen. Comparisons among GEX1 and GEX2 homologs from maize, rice (Oryza sativa), Arabidopsis, and poplar (Populus trichocarpa) revealed a core binding site for Dof transcription factors. The AtGEX1 and AtGEX2 promoters will be useful for manipulating gene expression in sperm cells, for localization and functional analyses of sperm proteins, and for imaging of sperm dynamics as they are transported in the pollen tube to the embryo sac.
Future Opportunities and Challenges with Using Demand Response as a Resource in Distribution System Operation and Planning Activities (2016)

This scoping study focuses on identifying the ability for current and future demand response opportunities to contribute to distribution system management. To do so, this scoping study will identify the needs of a distribution system to operate efficiently, safely and reliably; summarize both benefits and challenges for the operation of the distribution system with high penetration levels of distributed energy resources; define a suite of services based on those changing operational needs that could be provided by resources; identify existing demand response opportunities sponsored by distribution utilities and/or aggregators of retail customers; assess the extent to which distribution system services can be provided via DR opportunities both in their current form and with alterations to their design; and provide a qualitative assessment of coordination issues that bulk power and distribution system providers of DR opportunities will need to address.
A new simple system for measuring volume changes in unsaturated soils (2002)

A new simple system for accurately measuring overall total volume changes in unsaturated soil specimens with a triaxial apparatus is introduced in this paper. The basic principle of the measuring system is to record changes in the differential pressure due to changes in the water level inside an open-ended, bottle-shaped inner cell caused by volume change in the specimen and inside a reference tube using an accurate differential pressure transducer. Several important steps were taken to improve the accuracy and sensitivity of the measuring system. Detailed calibrations were carried out to account for apparent volume changes as a result of changes in cell pressure, fluctuation in the ambient temperatures, creep in the inner cell wall, and relative movement between the loading ram and the inner cell. The calibration results demonstrate that the measuring system is reasonably linear, reversible, and repeatable. The estimated accuracy of the measuring system is in the order of 32 mm 3 (or 0.04% volumetric strain for a triaxial specimen 38 mm in diameter and 76 mm in height) once the system is properly calibrated.Key words: unsaturated soils, volume-change measurement, open-ended, bottle-shaped, inner cell, differential pressure, calibration.
Biological monitoring of exposure to toluene diisocyanate (2004)

The biological monitoring methods and strategies presented in this report are useful for assessing exposure to TDI in practice.
Voltammetric Detection of Hydrogen Peroxide at Carbon Fiber Microelectrodes (2010)

Hydrogen peroxide is a reactive oxygen species that is implicated in a number of neurological disease states and that serves a critical role in normal cell function. It is commonly exploited as a reporter molecule enabling the electrochemical detection of nonelectroactive molecules at electrodes modified with substrate-specific oxidative enzymes. We present the first voltammetric characterization of rapid hydrogen peroxide fluctuations at an uncoated carbon fiber microelectrode, demonstrating unprecedented chemical and spatial resolution. The carbon surface was electrochemically conditioned on the anodic scan and the irreversible oxidation of peroxide was detected on the cathodic scan. The oxidation potential was dependent on scan rate, occurring at +1.2 V versus Ag/AgCl at a scan rate of 400 V.s(-1). The relationship between peak oxidation current and concentration was linear across the physiological range tested, with deviation from linearity above 2 mM and a detection limit of 2 muM. Peroxide was distinguished from multiple interferents, both in vitro and in brain slices. The enzymatic degradation of peroxide was monitored, as was peroxide evolution in response to glucose at a glucose oxidase modified carbon fiber electrode. This novel approach provides the requisite sensitivity, selectivity, spatial and temporal resolution to study dynamic peroxide fluctuations in discrete biological locations.
Mutagens: Some possible health impacts beyond carcinogenesis (1983)

Considerable attention is currently being given to the roles of environmental and endogenous mutagens in tumor induction and in tumor progression through the abilities of mutagens to induce one or another kind of somatic mutation. It is proposed here that mutagens, also through induction of somatic mutations, initiate a spectrum of additional, much more common, focal lesions that are important to the human condition. While individually they are of relative insignificance and thus neglected by many clinicians, the functional attributes of these focal lesions have important impacts on human physiology and thus contribute substantially to the process of aging. Additionally, one individual focus may progress to an extent that it produces clinically recognizable symptoms and may even jeopardize the carrier's life. The ultimate and developed expression of these lesions is heterogeneous, is far removed in time from initial induction, and relies on additional host and environmental factors for expression; therefore, the possible role of somatic mutation is readily overlooked. The focal lesions reviewed are fibrous atherosclerotic plaques, senile cataracts, and metaplasias. Literature that implicates metaplasias as precursor or as predisposing lesions in the ultimate formation of gallstones, duodenal ulcers, and adenocarcinomas is summarized.
3D RoboMET Characterization (2015)

The goal of this project is to generate 3D microstructural data by destructive and non-destructive means and provide accompanying characterization and quantitative analysis of such data. This work is a continuing part of a larger effort to relate material performance variability to microstructural variability. That larger effort is called “Predicting Performance Margins” or PPM. In conjunction with that overarching initiative, the RoboMET.3D™ is a specific asset of Center 1800 and is an automated serialsectioning system for destructive analysis of microstructure, which is called upon to provide direct customer support to 1800 and non-1800 customers. To that end, data collection, 3d reconstruction and analysis of typical and atypical microstructures have been pursued for the purposes of qualitative and quantitative characterization with a goal toward linking microstructural defects and/or microstructural features with mechanical response. Material systems examined in FY15 include precipitation hardened 17-4 steel, laser-welds of 304L stainless steel, thermal spray coatings of 304L and geological samples of sandstone.