gebhart/patrepeval huggingface dataset:
Expression of neurotrophins and their receptors in the mammalian ovary is developmentally regulated: changes at the time of folliculogenesis. (1995)

An emerging body of evidence suggests that neurotrophins not only promote neuronal survival and differentiation, but can also target nonneuronal cells for their actions. Neurotrophins initiate their biological effects by binding to cell membrane tyrosine kinase receptors of the trk protooncogene family. In addition, all neurotrophins recognize with similar affinity a different receptor molecule known as p75 nerve growth factor receptor (p75 NGFR) or low affinity NGFR, which appears to interact with the trk receptors to potentiate their response to neurotrophins. The mature mammalian ovary has been shown to synthesize several neurotrophins, including nerve growth factor (NGF), neurotrophin 3 (NT-3), and neurotrophin 4/5 (NT-4/5). The ovary also expresses some of the neurotrophin receptors, including p75 NGFR, trkB [the receptor for NT-4/5 and brain-derived neurotropic factor (BDNF)], and trkA (the NGF receptor). The present experiments were undertaken to determine whether neurotrophins and their receptors are expressed at the time of definitive ovarian histogenesis, and whether any of them exhibit a developmental pattern of expression related to the completion of folliculogenesis. Immunohistochemical identification of p75 NGFR in rat embryonic ovaries revealed that the receptor is predominantly expressed in mesenchymal cells. By gestational day 18, these cells have formed pockets that enclose presumptive pregranulosa cells and groups of oocytes into ovigerous cords. Immediately after birth, the ovigerous cords are subdivided, resulting in the abrupt formation of primordial follicles between 24-48 h after birth. Consistent with these observations, the p75 NGFR messenger RNA (mRNA) content increased after birth and remained elevated at the time of follicular assembly. The NGF and trkA genes showed a different pattern of expression, as the ovarian content of both NGF and trkA mRNA decreased at the time of folliculogenesis. In contrast to the drop in NGF and trkA mRNA expression, NT-4 mRNA levels increased at the time of follicular assembly, coinciding with the abrupt appearance of trkB mRNA. In situ hybridization showed that the increase in NT-4 mRNA expression occurred in a subpopulation of oocytes between 24-48 h after birth, and that the trkB gene became predominantly expressed at this time in epithelial pregranulosa cells. Substantial, but unchanging, levels of NT-3 mRNA and the mRNA encoding trkC, the preferred NT-3 receptor, were detected throughout the perinatal period examined. Very low and invariable levels of BDNF were also detected.(ABSTRACT TRUNCATED AT 400 WORDS)
An experimental model for congestive heart failure after encephalomyocarditis virus myocarditis in mice. (1982)

Severe myocarditis was induced in inbred BALB/c mice inoculated with the M variant of encephalomyocarditis (EMC) virus. The mortality rate was maximal on the fourth day, then decreased gradually, but increased again between the eleventh and fourteenth days. Gross myocardial lesions were seen on the surface of the ventricles in 62 of 125 mice (49.6%) after the fifth day. These myocardial lesions were observed more frequently in the dead mice (46 of 49, 93.9%). Cavity dimensions and wall thickness were measured in two groups of mice with myocarditis. On days 5-7, the cavity dimensions of the right (RV) and left (LV) ventricles in inoculated mice (0.92 +/- 0.51 mm and 1.21 +/- 0.18 mm, respectively) were significantly larger than those in controls (RV 0.54 +/- 0.17, LV 1.01 +/- 0.15; p less than 0.05). The wall thickness of the RV (0.46 +/- 0.09, controls 0.64 +/- 0.11; p less than 0.001) and the LV (0.97 +/- 0.13, controls 1.12 +/- 0.19; p less than 0.05) was significantly decreased. On days 8-14, dilatation of the LV was more pronounced (1.48 +/- 0.37, p less than 0.005) than during days 5-7, and the interventricular septum was also thinner. Pleural effusion, ascites and congestion of the lungs and liver were noted, and death seemed due to congestive heart failure. This study is the first documentation of congestive heart failure after viral infection is an experimental animal.
Growth condition dependence of morphology and electric properties of ZnO films on sapphire substrates prepared by molecular beam epitaxy (2003)

Zinc oxide (ZnO) films were grown on sapphire (112̄0) substrates by molecular beam epitaxy under oxygen radical irradiation. The effect of the growth conditions, including the Zn/O ratio supplied to the film surface, on the electrical properties of ZnO films was studied in relation to the film morphology. We found that the growth rate strongly depended on the Zn flux from the Knudsen cell and the optimum condition for high growth rate was very narrow. The grain size in the lateral direction increased with increasing growth rate in the thickness direction. The increase in growth rate, especially in the lateral direction, resulted in the carrier mobility increasing up to 42 cm2 V−1 s−1. The carrier concentration N was sensitive to the substrate temperature, while the value of N was not sensitive to the source supplying ratio Zn/O. We discuss the decrease of the carrier concentration with increasing substrate temperature in regard to the formation of nonequilibrium defects.
Preparation of 1-D nanoparticle superstructures with tailorable thicknesses using gold-binding peptide conjugates (2010)

We describe the preparation of new 1-D gold nanoparticle superstructures with tailorable thicknesses formed using a self-assembled gold-binding peptide conjugate template and examine how the synthesis and assembly mechanism impacts the organization of the superstructures.
Cryptic α‐toxin gene in toxic shock syndrome and septicaemia strains of <i>Staphylococcus aureus</i> (1990)

The majority of clinical isolates of Staphylococcus aureus that produce toxic shock syndrome toxin-1 (TSST-1) fail to express alpha-toxin, despite having a copy of the hla gene in the chromosome. The hla gene was cloned from an Hla- TSST-1+ strain, Todd 555, which had been isolated from a case of toxic shock syndrome in the USA. Of the 630 bases of the Todd 555 gene sequenced, 46 differed from the hla gene sequence of strain Wood 46. The defect in alpha-toxin expression was shown to be due to a nonsense mutation which converted a CAG glutamine codon in the equivalent position in the functional Wood 46 sequence to a TAG stop codon. The same mutation was present in the hla gene cloned from a human septicaemia strain (V37) isolated in Dublin. The nonsense mutation of Todd 555 was suppressed by the supE44 mutation in Escherichia coli resulting in haemolytic activity in cell lysates. Hybrid hla genes were formed by splicing fragments of hla from Todd 555 and Wood 46. Expression of one such chimaeric hla gene in S. aureus demonstrated that the Todd 555 hla gene has a functional agr-regulated promoter. The silent hla gene may be a cryptic gene in S. aureus.
cAMP Has Distinct Acute and Chronic Effects on Aquaporin-5 in Lung Epithelial Cells (2004)

Aquaporin-5 (AQP5) is present on the apical membrane of epithelial cells in various secretory glands as well as on the apical membrane of the airway epithelium, airway submucosal glands, and type 1 pneumocytes, where it can participate in respiratory tract water homeostasis. We examined the effects of cAMP on AQP5 distribution and abundance. When AQP5-expressing mouse lung epithelial cells were treated with cAMP or the beta-adrenergic agonist terbutaline, a biphasic AQP5 response was observed. Short term (minutes) exposure to cAMP produced internalization of AQP5 off of the membrane and a decrease in protein abundance. Both of these responses were blocked by inhibition of protein kinase A and the decrease in abundance was blocked by chloroquine, indicating lysosome-mediated degradation. Sustained cAMP exposure (hours) produced an increase in membrane localization and increased abundance; these effects were also blocked by protein kinase A inhibition. The beta-adrenergic agonist terbutaline produced changes in AQP5 abundance in mouse trachea and lung, consistent with our findings in cultured epithelial cells. Purified AQP5 protein was phosphorylated by protein kinase A but not protein kinase C or casein kinase II, and aquaporin-5 was phosphorylated in cultured cells after long term (but not short term) exposure to cAMP. These studies indicate that cAMP and beta-adrenergic agonists produce distinct short and long term effects on AQP5 distribution and abundance that may contribute to regulation of lung water homeostasis.
Genotype-environment interactions and the maintenance of polygenic variation. (1989)

Genotype-environment interactions may be a potent force maintaining genetic variation in quantitative traits in natural populations. This is shown by a simple model of additive polygenic inheritance in which the additive contributions of alleles vary with the environment. Under simplifying symmetry assumptions, the model implies that the variance of the phenotypes produced across environments by a multilocus genotype decreases as the number of heterozygous loci increases. In the region of an optimal phenotype, the mapping from the quantitative trait into fitness is concave, and the mean fitness of a genotype will increase with the number of heterozygous loci. This leads to balancing selection, polymorphism, and potentially high levels of additive genetic variance, even though all allelic effects remain additive within each specific environment. An important implication of the model is that the variation maintained by genotype-environment interactions is difficult to study with the restricted range of environments represented in typical experiments. In particular, if fluctuations in allelic effects are pervasive, as suggested by the extensive literature on genotype-environment interactions, efforts to estimate genetic parameters in a single environment may be of limited value.
A simulation system for diffusive oxidation of silicon: a two-dimensional finite element approach (1990)

A numerical approach to the simulation of two-dimensional local oxidation of silicon is presented. The key idea is the description of the oxidation as a three-component thermodynamic process involving silicon, silicon dioxide, and oxidant molecules. This results in a reactive layer of finite width, in contrast to the sharp interface between silicon and dioxide in the conventional formulation. The numerical approximation takes advantage of this description in a finite-element approach which models silicon, dioxide, and reactive layer as a whole, removing the necessity of tracking the interface with element edges. It is shown that a suitable parameter identification results in an interface motion equivalent to that of the Deal-Grove model. Numerical examples show the advantages of the approach.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Chromium: a review of environmental and occupational toxicology. (1985)

The following topics are covered in this brief review on the environmental and occupational toxicology of chromium: occurrence, production and uses of chromium and chromium compounds; experimental toxicology; chromium toxicity for man; hygienic and ecologic aspects of chromium contamination of the environment. The review provides a conclusive evidence which suggests that chromium, especially its hexavalent form, is both toxic and carcinogenic, but its trivalent form is physiologically essential in the metabolism of insulin. It is also emphasized that among the major sources of environmental chromium today are the cement industry and the increasingly widespread use of chromium compounds added as an anticorrosion admixture to a variety of cooling systems, e.g. in large power plants, which may greatly contribute to the overall pollution of outdoor air at the sites.
Chiral anilines: development of C2-symmetric, late-transition metal catalysts for isoselective 2-butene polymerizationElectronic Supplementary Information (ESI) available: X-ray data for (S,S,S,S-1a)NiBr2. See http://www.rsc.org/suppdata/cc/b3/b307659h/ (2003)

Chiral nickel catalysts were synthesized and used for the isoselective polymerization of trans-2-butene.
Capacity Strategy (2015)

Abstract Capacity strategy is the term applied to an interconnected set of decisions, which determine the long‐term capacity configuration of an organization.
Activating E17K mutation in the gene encoding the protein kinase AKT in a subset of squamous cell carcinoma of the lung (2008)

Somatic mutation (E17K) that constitutively activates the protein kinase AKT1 has been found in human cancer patients. We determined the role of the E17K mutation of AKT1 in lung cancer, through sequencing of AKT1 exon 4 in 105 resected, clinically annotated non-small cell lung cancer specimens. We detected a missense mutations G-->A transition at nucleotide 49 (that results in the E17K substitution) in two squamous cell carcinoma (2/36) but not in adenocarcinoma (0/53). The activity of the endogenous kinase carrying the E17K mutation immunoprecipitated by tumour tissue was significantly higher compared with the wild-type kinase immunoprecipitated by the adjacent normal tissue as determined both by in vitro kinase assay using a consensus peptide as substrate and by in vivo analysis of the phosphorylation status of AKT1 itself (pT308, pS473) or of known downstream substrates such as GSK3 (pS9/S22) and p27 (T198). Immunostaining or immunoblot analysis on membrane-enriched extracts indicated that the enhanced membrane localization exhibited by the endogenous E17K-AKT1 may account for the observed increased activity of mutant E17K kinase in comparison with the wild-type AKT1 from adjacent normal tissue. In conclusion, this is the first report of AKT1 mutation in lung cancer. Our data provide evidence that, although AKT1 mutations are apparently rare in lung cancer (1.9%), the oncogenic properties of E17K-AKT1 may contribute to the development of a fraction of lung carcinoma with squamous histotype (5.5%).
Fiber optic hydrophone: improved strain configuration and environmental noise protection (1979)

It is shown that the pressure sensitivity of a fiber-optic hydrophone is strongly dependent on the fiber's strain configuration. Longitudinal strain is found to be much more effective than uniform strain, and consequently modifications to the sensor's design are proposed. Environmental noise sources such as ocean motion and mechanical vibrations are then discussed, and a new double-cavity configuration, which is unaffected by those perturbations, is presented. A tunable-cavity detection method is finally proposed, and it is shown how this method can overcome problems related to drift of the point of operation, laser intensity fluctuations, and nonlinearity for high dynamic ranges.
Effects of fructose on the energy metabolism and acid-base status of the perfused starved-rat liver. A 31 phosphorus nuclear magnetic resonance study (1980)

Fructose metabolism has been studied with 31P n.m.r. in perfused livers from rats starved for 48h. The time course of changes in liver ATP, Pi and sugar phosphate (fructose l-phosphate) concentrations, and intracellular pH were followed in each perfusion after infusion of fructose to give an initial concentration of either 5mM or 10mM. Rapid falls in the concentrations of ATP and Pi and intracellular pH occurred after infusion of fructose, reaching a minimum after 4-5 min, which was lower in the 10mM group than in the 5mM group. These changes were accompanied by a rapid rise in fructose 1-phosphate, reaching a plateau also after 4-5 min. At both concentrations of fructose, after the early falls, some recovery of ATP, Pi and intracellular pH occurred; this was complete for Pi and intracellular pH in the 5mM-fructose experiments (within 12-30 min). Complete restoration of ATP to the pre-fructose value was not achieved in either the 5mM of 10mM groups. Measurements of the uptake of lactate by the liver indicated that the fall in intracellular pH was caused primarily by production of protons accompanying the formation of lactate from fructose with possibly a transient contribution generated during the rise in fructose 1-phosphate.
High heat load vacuum ultraviolet mirror development in Japan (1995)

Developments of vacuum ultraviolet (VUV) mirrors and optical elements for intense synchrotron radiation in Japan are reported. High heat load tests of chemical-vapor-deposited silicon carbide (CVD SiC) mirrors, 500 x 50 x 40 mm<sup>3</sup>, were successfully made for undulator radiation with heat flux of 0.4 W/mm<sup>2</sup>. As extensions of various CVD SiC mirrors developed at the Photon Factory, the largest CVD SiC mirror with sintered SiC substrate, 1000 x 140 x 25 mm<sup>3</sup>, and the thin CVD SiC mirror, 300 x 50 x 5 mm<sup>3</sup>, were fabricated by the SP-ring 8 project. Heat load tests of a CVD-SiC-based grating and a multilayer mirror exhibit possibilities of making high heat load optical elements in the VUV and soft x-ray (SX) regions. Measurements of optical properties of CVD SiC mirrors produced by different methods showed that the optical constants in the VUV and SX regions were almost equal to one another. A profilometer for measuring surface figures of large-size aspheric mirrors was constructed on the basis of the Twyman-Green interferometer with heterodyne phase detection method.
Fleabite Allergic Dermatitis: A Review and Survey of 330 Cases (1978)

Clinical signs of acute fleabite allergic dermatitis (FAD) in dogs included intense pruritus and erythema. Dogs with chronic FAD had diminished pruritus. The primary lesion of FAD was a papule. Secondary lesions (hyperkeratosis and hyerpigmentation) were common. Diagnosis of FAD was based on history of flea infestation and on type and location of lesions. Intradermal testing with glycerinated flea antigen was of little diagnostic value. Treatment of FAD included (1) breaking the flea life cycle in the indoor and kennel environment by vacuuming and washing bedding as well as by the use of aerosol insecticides for fumigation, (2) minimizing flea infestation on the dogs by using insecticidal dips, baths, and flea collars, and (3) hyposensitization with flea antigen.
Expression of Fas, p53 and AFP in development of human fetal germ cells <i>in vitro</i> (2002)

In the present study we employed a two-step culture system to study the expression of Fas, p53 and alpha-fetoprotein (AFP) in the development in vitro of human fetal germ cells. p53 mRNA was determined by Northern blotting, and Fas content was assessed by western blotting. RT-nested polymerase chain reaction (RT-nPCR) analysis was performed to determine the expression of AFP mRNA in different stages of fetal follicular development. Follicular cell apoptosis was evaluated by DNA fragmentation analyses (DNA ladder). The results showed that by day 7 of culture approximately one-sixth of fetal germ cells grew to class C oocytes (primary oocytes) from class B oocytes (primordial oocytes) or class A oocytes. On day 45 of culture, one-third of these primary follicles doubled in size. In the meantime, there was a high proportion apoptosis of follicular cells on days 35 or 45 of culture, as evident by a clear ladder pattern of DNA fragmentation upon electrophoretic analysis. Expression of Fas antigen and p53 mRNA increased in a time-dependent manner, while AFP mRNA was expressed on days 10 to 35, and disappeared on day 45. These results indicate that human fetal germ cells can develop in a two-step culture system and AFP may play an active role in the proliferation of these germ cells. At the late stage of follicular development in vitro, a number of follicular cells became apoptotic. Moreover, apoptosis may be the mechanism responsible for fetal germ cell regression and the Fas antigen and/or p53-mediated death pathway may be central in the induction of germ cell regression.
The Role of Stress in Brain Development: The Gestational Environment's Long-Term Effects on the Brain. (2012)

During gestation, the fetal brain develops dramatically as structures and connections form, providing the foundation for all future development. The fetal environment plays a critical role in these early neural processes, for better or for worse. Scientists now know that exposure to maternal stress can sometimes have deleterious effects on the fetus, depending on the cause, timing, duration, and intensity of stress. Fortunately, postnatal interventions, such as a secure parent-infant bond and an enriched environment, can buffer the potential negative consequences.
Low Loss Tunable Optical Filter using Silicon Photonic Band Gap Mirrors (2007)

A fiber-pigtailed tunable optical filter is described, based on silicon-air photonic bandgap mirrors. The high reflectance achievable in these mirrors allows a short Fabry-Perot cavity to be employed, such that lensed fibers provide sufficient collimation without additional focusing elements. Mirror surface orientation and quality are shown to be crucial to performance, and a two-stage etch process is used to satisfy this requirement. MEMS elecrostatic actuation of one mirror is used for tuning, and the final device achieves fiber-to-fiber insertion loss of 11 dB, passband width 3 nm, and tuning range ≈ 10 nm.
Possible role of gangliosides in the interaction of colony-stimulating factor with granulocyte-macrophage progenitor cells. (1985)

Our previous studies have shown that preincubation of murine bone marrow (BM) cells with cholera toxin (CT) or with its B subunit inhibited their responsiveness to colony-stimulating factor (CSF). Because ganglioside GM1 is a component of the CT receptor, the present study was undertaken to determine whether gangliosides interact with CSF and therefore might play a role in the binding sites for CSF. Preincubation of CSF with increasing concentrations of bovine-brain mixed gangliosides resulted in decreased numbers of colonies of BM-derived granulocyte-macrophage progenitor cells (CFU-C) in soft agar. The inhibitory effect of the gangliosides could be reduced by increasing the concentrations of CSF. Evidence for direct binding of CSF to gangliosides was obtained by affinity chromatography of CSF on gangliosides-sepharose beads. CSF activity was retained on the beads and could be eluted with 6 M guanidine HCl. Four different individual gangliosides (GM1, GM2, GD1a, GT1b) were tested for their inhibitory effect on CSF-induced clonal growth of CFU-C. GM1 was the most effective with a 50% inhibition (I50) of clonal growth at a concentration of 15 microM. while the other three gangliosides had slight inhibitory activity (I50 at a concentration greater than 100 microM). In addition, preincubation of BM cells with rabbit anti-GM1 antibodies before addition of CSF reduced the clonal growth of CFU-C to 45%. These data indicate that GM1 interacts with CSF and suggest that gangliosides may play a role in the interaction of CSF with CFU-C and that the binding site for CSF on the surface of these cells might either consist of or contain this ganglioside.