gebhart/patrepeval huggingface dataset:
Cisplatin Nephrotoxicity Involves Mitochondrial Injury with Impaired Tubular Mitochondrial Enzyme Activity (2012)

Cisplatin is a widely used antineoplastic agent. However, its major limitation is dose-dependent nephrotoxicity whose precise mechanism is poorly understood. Recent studies have suggested that mitochondrial dysfunction in tubular epithelium contributes to cisplatin-induced nephrotoxicity. Here the authors extend those findings by describing the role of an important electron transport chain enzyme, cytochrome c oxidase (COX). Immunohistochemistry for COX 1 protein demonstrated that, in response to cisplatin, expression was mostly maintained in focally damaged tubular epithelium. In contrast, COX enzyme activity in proximal tubules (by light microscopy) was decreased. Ultrastructural analysis of the cortex and outer stripe of the outer medulla showed decreased mitochondrial mass, disruption of cristae, and extensive mitochondrial swelling in proximal tubular epithelium. Functional electron microscopy showed that COX enzyme activity was decreased in the remaining mitochondria in the proximal tubules but maintained in distal tubules. In summary, cisplatin-induced nephrotoxicity is associated with structural and functional damage to the mitochondria. More broadly, using functional electron microscopy to measure mitochondrial enzyme activity may generate mechanistic insights across a spectrum of renal disorders.
Surgical Tools Recognition and Pupil Segmentation for Cataract Surgical Process Modeling (2012)

In image-guided surgery, a new generation of Computer-Assisted-Surgical (CAS) systems based on information from the Operating Room (OR) has recently been developed to improve situation awareness in the OR. Our main project is to develop an application-dependant framework able to extract high-level tasks (surgical phases) using microscope videos data only. In this paper, we present two methods: one method to segment the pupil and one to extract and recognize surgical tools. We show how both methods improve the accuracy of the framework for analysis of cataract surgery videos, to detect eight surgical phases.
A low-bias and sensitive small RNA library preparation method using randomized splint ligation (2020)

Small RNAs are important regulators of gene expression and are involved in human development and disease. Next generation sequencing (NGS) allows for scalable, genome-wide studies of small RNA; however, current methods are challenged by low sensitivity and high bias, limiting their ability to capture an accurate representation of the cellular small RNA population. Several studies have shown that this bias primarily arises during the ligation of single-strand adapters during library preparation, and that this ligation bias is magnified by 2'-O-methyl modifications (2'OMe) on the 3' terminal nucleotide. In this study, we developed a novel library preparation process using randomized splint ligation with a cleavable adapter, a design which resolves previous challenges associated with this ligation strategy. We show that a randomized splint ligation based workflow can reduce bias and increase the sensitivity of small RNA sequencing for a wide variety of small RNAs, including microRNA (miRNA) and tRNA fragments as well as 2'OMe modified RNA, including Piwi-interacting RNA and plant miRNA. Finally, we demonstrate that this workflow detects more differentially expressed miRNA between tumorous and matched normal tissues. Overall, this library preparation process allows for highly accurate small RNA sequencing and will enable studies of 2'OMe modified RNA with new levels of detail.
Detection and quantitation of simulated anaerobic bacteremia by centrifugation and filtration (1983)

Fresh human whole blood was inoculated with various anaerobic bacteria or with combinations of anaerobic, facultatively anaerobic, and aerobic microorganisms (3 to 28 microorganisms per ml). The seeded blood was then layered on a reduced Ficoll-Hypaque gradient (density, 1.093 g/ml) and centrifuged (400 X g) for 30 min at ambient temperature. The entire gradient (plasma, leukocytes, and Ficoll-Hypaque) was removed and filtered anaerobically through a 0.45-micron membrane filter. The filters were then placed on reduced chocolate Mueller-Hinton agar plates and incubated at 35 degrees C in humidified atmosphere containing 85% N2, 10% H, and 5% CO2 or in air containing 5% CO2. No statistically significant differences were detected between the numbers of microorganisms recovered (alone or in combination) by filtration and by direct culturing of the original inoculum. All organisms were detected within 30 h after filtration. This technique has excellent sensitivity.
Effect of High Salinity Water on Growth and Survival of Penaeid Postlarvae from the Red Sea at Different Stocking Densities (2005)

A study undertaken to assess the effect of high salinity water on the growth and survival of Penaeus monodon, P. indicus and P. semisulcatus postlarvae in cylindrical 5000-l fibreglass tanks for 60 days under monoculture in saline water from the Red Sea.Three different stocking densities tested were 50, 100 and 200 postlarvae/m 2 with initial weight of 0.09 g.The highest net weight gained, survival rates and feed conversion ratio were obtained in the lowest stocking density (50 postlarvae/m 2 ) in all three species.High salinity caused an effect on the growth, survival rates, and feed conversion ratio of the penaeid postlarvae.
Treatment of Unresectable and Metastatic Cutaneous Squamous Cell Carcinoma (2010)

Cutaneous squamous cell carcinoma (SCC) is an already common disorder with a rapidly increasing incidence. Treatment of early disease depends primarily on surgery or destructive techniques. In contrast to the frequency of early SCC, unresectable or metastatic SCC is relatively rare, but potentially life-threatening without clearly proven treatment options. Few rigorous studies of the treatment of advanced SCC have been undertaken. In the past, various agents have been explored in a limited fashion, including chemotherapy (cisplatin, fluoropyrimidines, bleomycin, doxorubicin), 13-cis-retinoic acid, and interferon-α2a. Clinical activity has been suggested by these trials, but their small sizes, heterogeneous patient populations, and lack of randomization have hindered the use of their results in defining treatment paradigms. Only one rigorous randomized trial has focused on cutaneous SCC. Enrolling 66 patients, that trial randomized patients at high recurrence risk to either observation or postoperative interferon-α2a and 13-cis-retinoic acid. This treatment did not improve time to recurrence or prevent secondary cutaneous SCC from developing. Though not in the metastatic setting, this study casts doubt on the ability of this regimen to control metastatic disease. Recently, agents targeting the human epidermal growth factor receptor (erlotinib, gefitinib, cetuximab) have displayed preliminary evidence of activity in phase II clinical trials and case series reports. Expression of this receptor is frequent in cutaneous SCC and appears to be prognostically adverse. Only the conduct of rigorous trials, with well-defined endpoints, adequate patient numbers, and preferably randomization, can prove the clinical efficacy of this promising treatment approach and define better therapy for this vexing clinical problem.
Highly Branched Poly(<scp>l</scp>-lysine) (2003)

This paper describes the synthesis of several novel water-soluble highly branched polypeptides. The synthesis starts with the ring-opening polymerization of epsilon-benzyloxycarbonyl-l-lysine N-carboxyanhydride (Z-Lys NCA) or epsilon-trifluoroacetyl-l-lysine N-carboxyanhydride (TFA-Lys NCA), followed by end functionalization of the peptide chain with N(alpha),N(epsilon)-di(9-fluorenylmethoxycarbonyl)-l-lysine (N(alpha),N(epsilon)-diFmoc Lys). Deprotection of the N(alpha),N(epsilon)-diFmoc Lys end group affords two new primary amine groups that can initiate the polymerization of a second generation of branches. Repetition of this ring-opening polymerization-end functionalization sequence affords highly branched poly(epsilon-benzyloxycarbonyl-l-lysine) (poly(Z-Lys)) and poly(epsilon-trifluoroacetyl-l-lysine) (poly(TFA-Lys)) in a small number of straightforward synthetic steps. Removal of the side-chain protective groups yields water-soluble and highly branched poly(l-lysine)s, which may be of potential interest for a variety of medical applications.
The human foamy virus internal promoter directs the expression of the functional Bel 1 transactivator and Bet protein early after infection (1994)

The human foamy virus or spumaretrovirus (HFV) is a complex retrovirus that has the capacity to code not only for the three retroviral genes gag, pol, and env but, in addition, for at least three bel genes. The HFV provirus contains two different and functionally active promoters: the classical retroviral promoter in the 5' long terminal repeat and a recently identified second promoter in the env gene upstream of the bel genes. Both promoter/enhancers are strongly dependent on the HFV transcriptional transactivator protein Bel 1. Here we report that the internal promoter directs the synthesis of viral transcripts that code for functionally active Bel 1 and for Bet proteins that appeared early after HFV infection. The viral mRNAs of the internal promoter have a 112-nucleotide-long leader exon and were spliced predominantly at the first splice donor site in the 5' untranslated region. The data were obtained by transient expression assays, transactivation experiments, and RNA analyses of transcripts derived from HFV-infected cells. The results provide strong evidence for the crucial role the internal promoter plays during HFV infection in generating bel-specific transcripts.
Identification of linear parameter varying systems using an iterative deterministic-stochastic subspace approach (2007)

In this paper we introduce a recursive subspace system identification algorithm for MIMO linear parameter varying systems driven by general inputs and a white noise time varying parameter vector. The new algorithm is based on a convergent sequence of linear deterministic-stochastic state-space approximations, thus considered a Picard based method. Such methods have proven to be convergent for the bilinear state-space system identification problem. The key to the proposed algorithm is the fact that the bilinear term between the time varying parameter vector and the state vector behaves like a white noise process. Using a linear Kalman filter model, the bilinear term can be efficiently estimated and then used to construct an augmented input vector at each iteration. Since the previous state is known at each iteration, the system becomes linear, which can be identified with a linear-deterministic subspace algorithm such as MOESP, N4SID, or CVA. Furthermore, the model parameters obtained with the new algorithm converge to those of a linear parameter varying model. Finally, the dimensions of the data matrices are comparable to those of a linear subspace algorithm, thus avoiding the curse of dimensionality.
Cyanoethylation of Alpha Amino Acids. I. Monocyanoethyl Derivatives<sup>2</sup> (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCyanoethylation of Alpha Amino Acids. I. Monocyanoethyl Derivatives2L. L. McKinney, E. H. Uhing, E. A. Setzkorn, and J. C. CowanCite this: J. Am. Chem. Soc. 1950, 72, 6, 2599–2603Publication Date (Print):June 1, 1950Publication History Published online1 May 2002Published inissue 1 June 1950https://pubs.acs.org/doi/10.1021/ja01162a069https://doi.org/10.1021/ja01162a069research-articleACS PublicationsRequest reuse permissionsArticle Views321Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cost analysis of regular and filgrastim treatment in patients with refractory chronic rhinosinusitis. (2002)

In this double blind randomized placebo controlled clinical trial of filgrastim in chronic sinusitus, we analyzed costs of a 24-week interval in which filgrastim was administered. Since we hypothesized that the scheduled preventive visits within the trial might cause savings as compared to the regular situation in which these patients have a strong tendency to visit the outpatient clinic immediately in case of complications, direct medical costs within the trial were also compared to costs of regular treatment. The difference in costs between both trial groups was driven by the filgrastim costs (Euro 5108). If filgrastim costs were left out of consideration, no significant difference in direct medical costs remained between the filgrastim and placebo groups (Euro 2904 and Euro 2765, respectively). Indirect medical costs also showed no significant differences. Within a regular situation, costs of a 24-week interval were Euro 896. As filgrastim treatment had already been shown not to improve the quality of life, its cost-effectiveness in these patients can only be favourable in case of major clinical improvements. Furthermore, scheduled preventive visits in chronic sinusitis patients appear not to cause savings as compared to the situation in which patients are only seen in case of recurrences.
Refugees are not Migrants (2005)

Journal Article Refugees are not Migrants Get access Erika Feller Erika Feller Director, Department of International Protection United Nations High Commissioner for Refugees Search for other works by this author on: Oxford Academic Google Scholar Refugee Survey Quarterly, Volume 24, Issue 4, 2005, Pages 27–35, https://doi.org/10.1093/rsq/hdi077 Published: 01 December 2005
Low-energy electron detection with delta-doped CCDs (1997)

Delta-doped CCDs have achieved stable quantum efficiency, at the theoretical limit imposed by reflection from the Si surface in the near UV and visible. In this approach, an epitaxial silicon layer is grown on a fully-processed CCD using molecular beam epitaxy. During the silicon growth on the CCD, 30 percent of a monolayer of boron atoms are deposited nominally within a single atomic layer, resulting in the effective elimination of the backside potential well. In this paper, we will briefly discuss delta-doped CCDs and their application of low-energy electron detection. We show that modification of the surface this way can greatly improve sensitivity to low-energy detection. We show that modification of the surface this way can greatly improve sensitivity to low-energy electrons. Measurements comparing the response of delta-doped CCDs with untreated CCDs were made in the 50 eV-1.5 keV energy range.For electrons with energies below 300 eV, the signal from untreated CCDs was below the detection limit for our apparatus, and data are presented only for the response of delta-doped CCDs at these energies. The effects of multiple electron hole pair production and backscattering on the observed signals are discussed.
Effects of Thyroid Hormone Analogs on Lipid Metabolism and Thermogenesis (2008)

Thyroid hormone affects in a myriad of biological processes such as development, growth, and metabolic control. Triiodothyronine (T3) is the biologically active form of thyroid hormone that acts through nuclear receptors, TRalpha and TRbeta, regulating gene expression. Given that the distribution of these receptors is heterogeneous amongst the different tissues, it is not surprising that some physiological effects of T3 are isoform specific. For example, while TRalpha is the dominant receptor in the brain and skeletal system and mediates most of the synergism between T3 and the sympathetic signaling pathway in the heart, TRbeta is abundant in liver and is probably the isoform that mediates most of the T3 effects on lipid metabolism. Thus, it makes sense to develop compounds that selectively act on either one of the TRs, allowing for the activation of specific T3-dependent pathways. This article reviews the recent progress made in this area, focusing on the physiological effects of compounds that lower serum cholesterol and decrease fat mass, as they spare skeletal muscle and bone masses, as well as the heart. The available studies indicate that achieving selective activation of different TR-mediated pathways is a promising strategy for treating lipid disorders and obesity.
Industry Analysis of the Stationary Sector in India: A Market Acceptance Study (2014)

The writing instruments market in India is a very lucrative market for various brands worldwide simply because of its sheer size. This market is estimated at 1600 to 2400 mn pieces a year and Rs 22 bn in value. A number of Indian as well as International players are in the race to tap this market to the fullest. This paper is the result of a through field study done over a period of 11 months. Most of the data in this paper comes from primary sources that include consumer surveys, retail surveys, lab experiments and expert opinions. The research Methodology is detailed in subsequent chapters. This study primarily aims at mapping the Indian stationary market to achieve its objectives. Objectives that include gauging market from a macro perspective, analyzing the brand preferences, the consumer buying behavior, retailer BTL activities etc. The ultimate objective of the study is to study the user acceptance of various parameters by mapping this market. This Research Paper is aimed at presenting the findings of a thorough primary and secondary work done to explore the market acceptance for a new entrant. Also, this study throws light on the Consumer behavior and retail (POP) factor for writing instruments industry.
Radiation Protection at Medical Accelerators (2001)

This paper focuses on radiation protection at proton and light ion accelerators for radiotherapy. The National Centre of Hadrontherapy, which is planned to he built in Italy in the next five years, is considered as a reference facility for applying the various methodologies presented. The shielding design is firstly discussed, together with that of the access maze to the treatment rooms. Subsequently, the main aspects for the estimate of the air activation in the environment hosting the accelerator system are described. The estimate of the dose equivalent due to the activated air transferred to the neighbourhood population is also treated. Outlines are given of the radioactivity induced by the primary beam in the materials constituting the magnets and the patient's personal collimator.
Relationship between Antibiotic duration and Superficial Surgical Site Infection in Arthroplasty surgery, Khartoum, Sudan (2022)

The study aims to study the difference in using antimicrobial prophylaxis for different durations in joints replacement operations and developing superficial surgical site infection.
Purification and functional properties of simian virus 40 large and small T antigens overproduced in insect cells (1988)

The insect baculovirus Autographa californica nuclear polyhedrosis virus was used as an expression vector for the simian virus 40 (SV40) small t (t) and large T (T) antigens. Spodoptera frugiperda (SF9) cells infected with recombinant viruses encoding these proteins produced approximately 1 to 2 micrograms of t and up to 30 micrograms of T per 3 X 10(6) cells. The former was highly soluble after Nonidet P-40 extraction of the infected cells, unlike its Escherichia coli-produced counterpart. Both SF9-produced proteins were of authentic size and could be readily immunoprecipitated by specific antibodies. Single-step immunoaffinity chromatography was used to purify the two proteins to near homogeneity, with yields averaging 70% in each case. Experiments to test the biological activity of the baculovirus SV40 proteins showed that SF9 t was capable of associating with two of the cellular proteins reported to bind to t in SV40-infected mammalian cells. Moreover, SF9 T had ATPase activity comparable to that of T produced in monkey cells, exhibited helicase activity and SV40 origin-specific DNA binding, and was active in the SV40 DNA replication assay in vitro. Thus, the SV40 T antigens produced in insect cells can be used in future studies of their biochemical roles in vitro and in vivo.
Real-time measurement of the NO<sub>2</sub>concentration in ambient air using a multi-mode diode laser and cavity enhanced multiple line integrated absorption spectroscopy (2015)

We report on the measurement of the NO<sub>2</sub> concentration in ambient air using a highly sensitive trace gas detector based on a simplified design that uses a multi-mode diode laser and cavity enhanced multiple line integrated absorption spectroscopy. The broad frequency range of the laser covers a large number of absorption lines of NO<sub>2</sub> and excites a large number of cavity modes. The off-axis cavity arrangement reduces the detector’s susceptibility to vibration. The detector was calibrated using known concentrations of NO<sub>2</sub> in zero air and used to measure the concentration of NO<sub>2</sub> in ambient air in our laboratory. A sensitivity of 75 ppt was achieved.
Linear and nonlinear optical responses influenced by broken symmetry in an array of gold nanoparticles (2004)

An array of low-symmetry, L-shaped gold nanoparticles is shown to exhibit high sensitivity to the state of incident polarization. Small imperfections in the shape of the actual particles, including asymmetric arm lengths and edge distortions, break the symmetry attributed to an ideal particle. This broken symmetry leads to a large angular displacement of the extinction axes from their expected locations. More significantly, second-harmonic generation experiments reveal significant second-order susceptibility tensor components forbidden to the ideal symmetry.
The Student - as - Customer Metaphor: A Deconstruction Using Foucauldian Constructs (2016)

This paper examines the social impact of the 'student-as-customer' metaphor on the role of academic leadership and objectivity of the assessment process in the higher education sector. A hybrid approach is used in which Deconstruction seeks to render visible the Foucauldian constructs of power and privilege. The paper draws on the existing literature to explore the problematic relationship between the student as customer metaphor, academic leadership, and student assessment. The paper raises awareness of the inherent tensions with the present reliance on the student as customer metaphor and the resulting negative impact on both students and academic staff. This addresses a gap in the literature in regards to the effect that the use of the student-as- customer metaphor has on academic leadership and assessment in higher education.