gebhart/patrepeval huggingface dataset:
A dipstick immunoassay to rapidly measure serum oestrone sulfate concentrations in horses (2001)

A dipstick, competitive immunoassay for rapidly measuring serum oestrone sulfate (OS) concentrations in horses was developed to distinguish mares 100 or more days pregnant from non-pregnant animals. 6-Ketoestrone 6-carboxymethyloxime conjugated to bovine serum albumin (oestrone CMO-BSA) was 'dotted' 25 mm from the bottom edge of 45 x 5 mm strips of polyester-film-supported cellulose nitrate membrane, pore size 3 microm. The strips were blocked, dried and a 15 x 5-mm cellulose absorbent sink attached 10 mm from the top of each strip. The manufactured dipsticks were stored with desiccant at room temperature until used. A monoclonal antibody recognizing OS was coated onto uniform, blue-dyed polystyrene microspheres (mean diameter, 0.31 microm) by adsorption. After blocking, several washes and resuspension by sonication, the antibody-coated microspheres were stored at 4 degrees C. The concentrations of oestrone CMO-BSA dotted onto the dipsticks and OS antibody coated onto the microspheres were optimized to produce a test that allowed maximum discrimination between the concentrations of OS found in serum of mares 100 or more days pregnant (i.e. >30 ng OS mL(-1)) relative to those found in non-pregnant mares (i.e. <10 ng OS mL(-1)). To perform the dipstick test, 30 microL of carrier buffer, 10 microL of OS antibody-coated microspheres and 10 microl of OS standard or serum sample were pipetted into a microwell and mixed. A dipstick was placed in the solution. All the liquid migrated up the dipstick into the absorbent sink within 15-20 min leaving a blue dot where the OCMO-BSA had been placed. The intensity of colour of the blue dot, which correlated inversely with the concentration of OS in the standard or serum sample, was assessed visually and by computer image analysis. An OS concentration less than 5 ng mL(-1) produced a deep blue dot, 20 ng/ml a light blue dot and a concentration greater than 50 ng mL(-1) a very faint blue dot, or none at all. Serum samples from 42 non-pregnant mares and 40 mares over 100 days pregnant were analysed by the dipstick test. All the serum samples from non-pregnant mares produced dipsticks with deep blue dots that ranged in intensity from 20 to 38 colour intensity units, equivalent to OS concentrations less than 7 ng mL(-1). Sera from all the pregnant mares generated dipsticks with either faint blue dots or none at all (i.e. < or =5 colour intensity units, equivalent to OS concentrations >40 ng mL(-1). It is concluded that this novel, rapid dipstick immunoassay offers a practical, alternative means of analysing serum OS concentrations in horses, and enables mares that are 100 or more days pregnant to be distinguished from those that are not pregnant.
Monoclonal antibody 45-2D9 conjugated to the A chain of ricin is specifically toxic to c-Ha-ras-transfected NIH 3T3 cells expressing gp74. (1986)

Monoclonal antibody 45-2D9 recognizes a 74K Mr glycoprotein determinant on a c-Ha-ras oncogene-transfected cell line (45-342). An immunotoxin was made by conjugating this antibody to the A chain of ricin toxin (RTA). The immunotoxin could mediate essentially complete inhibition of leucine and thymidine incorporation by 45-342 cells prepared as single cell suspensions from tumors grown in vivo. Addition of ammonium chloride to the culture medium potentiated this inhibition, but the magnitude of this effect was dependent on incubation time and cell concentration. The immunotoxin effects were noted at concentrations 100-fold lower than similar effects caused by unconjugated RTA, and the 45-2D9 antibody had no effect in the assay system. Immunotoxins directed against antigens not expressed by 45-342 were not effective, and the 45-2D9 immunotoxin was not specifically toxic to other transfected cells not expressing the gp74 antigen. After a 72-hr incubation, lysis of 80% of the 45-342 cells was demonstrated by trypan blue exclusion. Complete inhibition of 45-342 colony formation was achieved at 10 days with a 10(-9) M concentration of the specific immunotoxin. These results indicate that an immunotoxin with specific reactivity towards an oncogene-transformed cell can be made, and that such cells derived from fresh tumors are susceptible to immunoconjugate-mediated toxicity.
Protective systems. Part III: Protective systems for rural distribution up to 33 kV (1944)

The importance of installing suitable and efficient protective apparatus on rural networks, at all stages of their development is emphasized.A typical protective scheme for an 11-kV and 33-kV rural distribution system which has been put into operation in a particular area of supply is described, and the reasons leading to its adoption are given. The practical difficulties met with in securing efficient operation and of carrying out proper maintenance are discussed. Some statistical records of fault interruptions are presented. These indicate the number and type of faults causing total or partial failures of supply and show the conditions under which the protective gear is called upon to function.The nature of the faults which occur on rural networks is analysed and a suggested standard form of keeping fault statistics is put forward for general adoption by undertakings distributing in rural areas.
Influence of intestinal transit time on azo-reduction of salicylazosulphapyridine (Salazopyrin). (1979)

During a normal and an accelerated intestinal transit, in seven healthy volunteers, the recoveries of salicylazosulphapyridine (SASP) and its split products sulphapyridine (SP) and 5-aminosalicylic acid (5-ASA) were determined in urine and faeces. The azo-reduction of SASP and consequently the recovery of 5-ASA in the faeces was found to be substantially decreased during an accelerated intestinal transit. In addition, in 18 patients with inflammatory disease of the colon during maintenance therapy of SASP it could be demonstrated that the serum SP levels were related to the diarrhoeal state and did not correlate with disease activity. As recent studies have reported that 5-ASA is possibly the active therapeutic moiety of SASP, the ineffectiveness of SASP therapy in patients with active colitis may be ascribed to the reduced azo reduction of SASP as the result of profuse diarrhoea.
‘Squeezed’ interparticle properties for plasmonic coupling and SERS characteristics of duplex DNA conjugated/linked gold nanoparticles of homo/hetero-sizes (2016)

The formation of interparticle duplex DNA conjugates with gold nanoparticles constitutes the basis for interparticle plasmonic coupling responsible for surface-enhanced Raman scattering signal amplification, but understanding of its correlation with interparticle spatial properties and particle sizes, especially in aqueous solutions, remains elusive. This report describes findings of an investigation of interparticle plasmonic coupling based on experimental measurements of localized surface plasmon resonance and surface enhanced Raman scattering characteristics for gold nanoparticles in aqueous solutions upon introduction of interparticle duplex DNA conjugates to define the interparticle spatial properties. Theoretical simulations of the interparticle optical properties and electric field enhancement based on a dimer model have also been performed to aid the understanding of the experimental results. The results have revealed a 'squeezed' interparticle spatial characteristic in which the duplex DNA-defined distance is close or shorter than A-form DNA conformation, which are discussed in terms of the interparticle interactions, providing fresh insight into the interparticle double-stranded DNA-defined interparticle spatial properties for the design of highly-sensitive nanoprobes in solutions for biomolecular detection.
Role of the <i>leu-3</i> Cistron in the Regulation of the Synthesis of Isoleucine and Valine Biosynthetic Enzymes of <i>Neurospora</i> (1974)

The production by Neurospora of the enzymes of isoleucine and valine synthesis in response to specific end product-derived signals depends upon the presence of an effective leu-3 regulatory product and its effector alpha-isopropylmalate (alpha-IPM). In leu-3(+) strains, threonine deaminase production is repressed as a function of available isoleucine, acetohydroxy acid synthetase as a function of valine, and the isomeroreductase and dihydroxy acid dehydratase as a function of isoleucine and leucine. In the absence of an effective leu-3 regulatory product, alpha-isopropylmalate, or both, the production of isoleucine and valine biosynthetic enzymes is fixed at or near fully repressed levels even under conditions of severe end product limitation. Thus, in addition to its involvement in the regulation of expression of the three structural genes of leucine synthesis, the leu-3 alpha-IPM regulatory product is necessary for full expression of at least four genes specifying the structure of the enzymes of isoleucine and valine synthesis. It is suggested that the leu-3 alpha-IPM regulatory element may facilitate transcription of the genetically dispersed cistrons either by imposing specificity on ribonucleic acid polymerase for structurally similar promoters adjacent to each of the cistrons or by "opening" promoters after interaction with nearly identical stretches of deoxyribonucleic acid near each of the structural genes.
Multi-channel pulse oximetry for wearable physiological monitoring (2013)

Pulse oximetry is a widely accepted clinical method for noninvasive monitoring of arterial oxygen saturation and pulse rate. Significant improvements aimed at curbing motion artifacts and improving reliability in detecting sufficiently strong photoplethysmographic signals are required to reduce errant measurements before the pulse oximeter can be considered for wider mobile applications. The present work describes the development of a wearable multi-channel reflectance pulse oximeter to investigate if a motion artifact-free signal can be obtained in at least one of the multichannels at any given time. Pilot findings provided a proof of concept to support the hypothesis that photoplethysmograms acquired concurrently from independent channels in a multi-channel pulse oximeter sensor respond differently to motion artifacts, thus laying the foundation for future development of robust active noise cancellation and data fusion based algorithms to mitigate the effects of motion artifacts.
Distribution and accumulation of dietary ergothioneine and its metabolites in mouse tissues (2018)

L-ergothioneine (ET) is a diet-derived amino acid that accumulates at high concentrations in animals and humans. Numerous studies have highlighted its antioxidant abilities in vitro, and possible cytoprotective capabilities in vivo. We investigated the uptake and distribution of ET in various organs by a highly sensitive and specific liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) technique, both before and after oral administration of pure ET (35 and 70 mg/kg/day for 1, 7, and 28 days) to male C57BL6J mice. ET primarily concentrates in the liver and whole blood, and also in spleen, kidney, lung, heart, intestines, eye, and brain tissues. Strong correlations were found between ET and its putative metabolites - hercynine, ET-sulfonate (ET-SO<sub>3</sub>H), and S-methyl ET. Hercynine accumulates in the brain after prolonged ET administration. This study demonstrates the uptake and distribution of ET and provides a foundation for future studies with ET to target oxidative damage in a range of tissues in human diseases.
Review: cytokines and malaria. (1990)

Malaria, which is caused by hemoprotozoan parasites of the genus Plasmodium, has once again reached epidemic proportions. The resurgence of malaria has occurred because the parasite has developed resistance to the anti-malarial drugs and the mosquito vector has developed resistance to the insecticides. Added to these impediments is the problem that, in spite of intense efforts by researchers world-wide, there is yet no effective anti-malarial vaccine. Our lack of knowledge concerning the exact mechanism of the host immune response to infection with Plasmodium parasites has contributed significantly to the lack of an effective and safe vaccine. The role of an antibody-independent, cell-mediated mechanism which can result in the generation of soluble mediators or cytokines by T lymphocytes and macrophages in host defense against blood stage malaria is being actively investigated in humans and in mice with malaria. With the availability of recombinant lymphokines and monokines and neutralizing antibodies against these reagents it is now possible to determine the role of cytokines in the development of protective anti-malarial immunity. In this review, we discuss recent evidence from human studies and experimental murine models concerning the possible roles of cytokines in malaria.
Image collapse issues in photoresist (2001)

The continued shrinkage of line widths below 150nm has not been without concerns of the mechanical stability of the resist structure. The development of the exposed resist undergoes many phase changes such as solid solubilization into a liquid followed by drying by volatilization. In the development of features especially with aspect ratio (AR) >3 and for lines/spaces (l/s)<1:2, the phase changes create interfacial tensions at the liquid/solid and gas/liquid/solid surfaces that can cause image collapse (IC). Aqueous based resist are particularly susceptible to collapse due to the high surface tension of the rinse water (72 mN/m). Two major steps to reduce or eliminate the IC are to strengthen the mechanical stability of the resist or to reduce the surface tension of the drying/rinse liquid. We have investigated various methods to reduce the surface tension of the water rinse either by lowering the surface tension of the rinse liquid or by removal of the rinse water prior to drying. Positive ArF and KrF resist and negative resist have been investigated. To reduce the surface tension of rinse liquid, the addition of surfactant followed by drying is a direct method which may be used. Indirectly, the prior removal of water by solvents or emulsification with inert components followed by drying in the supercritical fluid of CO<SUB>2</SUB> can maintain high aspect ratio images at AR>3. Negative resist are more applicable to process as they can tolerate a wider variety of co- solvents of CO<SUB>2</SUB> phillic surfactants.
Measuring Direct Thrombin Inhibitors With Routine and Dedicated Coagulation Assays (2012)

The use of direct thrombin inhibitors (DTIs) for prophylactic or therapeutic anticoagulation is increasing because of the predictable bioavailability and short half-life of these DTIs. However, in certain situations, indication of the concentration is warranted. We investigated the effects of 3 DTIs (lepirudin, argatroban, and bivalirudin) in 6 pooled plasma specimens on routine coagulation assays (activated partial thromboplastin time [aPTT], prothrombin time [PT], and thrombin time [TT]) and dedicated DTI assays (Hemoclot, HemosIL, the ecarin clotting time, and a chromogenic ecarin clotting time) on 2 coagulation analyzers. We found routine tests to be nondiscriminative between concentrations of different DTIs in the aPTT. Moreover, for PT and TT, the responses for different DTIs differed. This was similar for ecarin clotting assays. The Hemoclot and HemosIL assays showed identical linear increases for all 3 DTIs. We conclude that dedicated calibrated assays based on a diluted TT (Hemoclot and HemosIL) appear to be the most suitable for monitoring purposes.
Fault-tolerant high-performance CORDIC processors (2002)

This paper presents a low-cost approach to concurrent error detection in a high-performance CORDIC processor based on a conditional-sum scheme. The specific characteristics of the CORDIC computation and the processor allow fault detection at a low increase in circuit complexity and latency. The detection scheme is based on use of the AN codes for the arithmetic part and on duplication of the rotation direction generators. Granular-pipelining has been applied to provide a variety of different performance tradeoffs, all with the same fault detection capabilities.
Increased Nuclear Apoptosis-Inducing Factor after Transient Focal Ischemia: A 12/15-Lipoxygenase-dependent Organelle Damage Pathway (2010)

12/15-lipoxygenase (12/15-LOX) contributes to acute neuronal injury and edema formation in mouse models of middle cerebral artery occlusion (MCAO). The apoptosis-inducing factor (AIF) is implicated in caspase-independent forms of apoptosis, and has been linked to ischemic neuronal cell death. We show here that increased AIF in the peri-ischemic cortex of mouse colocalizes with 12/15-LOX after 2 h of MCAO. The 12/15-LOX inhibitor baicalein prevents the increase and nuclear localization of AIF, suggesting this pathway may be partially responsible for the neuroprotective qualities of baicalein. Using an established cell line model of neuronal oxidative stress, we show that 12/15-LOX activated after glutathione depletion leads to AIF translocation to the nucleus, which is abrogated by the 12/15-LOX inhibitor baicalein (control: 19.3%+/-6.8% versus Glutamate: 64.0%+/-8.2% versus glutamate plus baicalein: 11.4%+/-2.2%). Concomitantly, resident proteins of the ER are dispersed throughout the cell (control: 31.0%+/-8.4% versus glutamate: 70.0%+/-5.5% versus glutamate plus baicalein: 8.0%+/-2.7%), suggesting cell death through organelle damage. Taken together, these findings show that 12/15-LOX and AIF are sequential actors in a common cell death pathway that may contribute to stroke-induced brain damage.
Multiscale mechanobiology: mechanics at the molecular, cellular, and tissue levels (2013)

Mechanical force is present in all aspects of living systems. It affects the conformation of molecules, the shape of cells, and the morphology of tissues. All of these are crucial in architecture-dependent biological functions. Nanoscience of advanced materials has provided knowledge and techniques that can be used to understand how mechanical force is involved in biological systems, as well as to open new avenues to tailor-made bio-mimetic materials with desirable properties.In this article, we describe models and show examples of how force is involved in molecular functioning, cell shape patterning, and tissue morphology.
Soft Combination and Detection for Cooperative Spectrum Sensing in Cognitive Radio Networks (2007)

In this paper, we consider cooperative spectrum sensing based on energy detection in cognitive radio networks. Soft combination of the observed energy values from different cognitive radio users is investigated. Maximal ratio combination (MRC) is theoretically proved to be nearly optimal in low signal- to-noise ratio (SNR) region, an usual scenario in the context of cognitive radio. Both MRC and equal gain combination (EGC) exhibit significant performance improvement over conventional hard combination. Encouraged by the performance gain of soft combination, we propose a new softened hard combination scheme with two-bit overhead for each user and achieve a good tradeoff between detection performance and complexity. While traditionally energy detection suffers from an SNR wall caused by noise power uncertainty, it is shown in this paper that an SNR wall reduction can be achieved by employing cooperation among independent cognitive radio users.
Expression of angiogenin in E.coli and its purification and activity (2005)

Objective: To obtain the recombinant augiogenin (Ang) protein by means of prokaryotic expression system and investigate its bioactivity. Methods:An high-efficiency fusion expression vector pGEX-4T-Ang was constructed, the induced protein of interest was purified by affinity-chromatography. The non-fusion recombinant human angiogenin was obtained by cleavage, and the specificity of the expressed product was examined by Western blotting. The biological functions of the recombinant protein was examined by means of a classical test of chicken embryonic allantoic vessel proliferation.Results:The expressed protein of interest amounted to 20% of the total bacterial protein, and the affinity chromatography-purified protein reached a purity over 90%. The protein could specifically react to the antibody against Ang, and possesses an obvious activity promoting angiogenesis. Conclusion: The angiongenin expressed by prokaryotic system possesses a good bioactivity.
Defense Responses in Plants and Animals—More of the Same (1998)

Pathogens are a ubiquitous fact of life, and the ability to resist their attentions constitutes one of the keys to biological success. However, as each defensive innovation is established in the host, new ways to circumvent it evolve in the pathogen. Over time, these coevolutionary struggles between
PROPERTIES OF HYDRIDED ZIRCONIUM (1959)

The properties of hydrided zirconium at hydrogen contents of 2.70, 3.40, and 4.00 NH are presented, although data were not available for all of the properties at each NH. Dath obtained under GE-ANPD authorization and from available literature were compiled. The properties reported consist of density, tensile strength, and hardness from room temperature to 1600 deg F, creep and stress rupture from 800 to 1600 deg F, linear thermal expansion from room temperature to 1400 deg F, specific heat from 32 to 1400 deg F, and thermal conductivity and electrical resistivity from 100 to 1000 deg F. (auth)
Classification of Normal and Dysphagic Swallows by Acoustical Means (2004)

This paper proposes a noninvasive, acoustic-based method to differentiate between individuals with and without dysphagia or swallowing dysfunction. Swallowing sound signals, both normal and abnormal (i.e., at risk of some degree of dysphagia) were recorded with accelerometers over the trachea. Segmentation based on waveform dimension trajectory (a distance-based technique) was developed to segment the nonstationary swallowing sound signals. Two characteristic sections emerged, Opening and Transmission, and 24 characteristic features were extracted and subsequently reduced via discriminant analysis. A discriminant algorithm was also employed for classification, with the system trained and tested using the leave-one-out approach. Overall, 350 signals were used from three bolus consistencies (semisolid, thick and thin liquids). A final screening algorithm correctly classified 13 of 15 control subjects and 11 of 11 subjects with some degree of dysphagia and/or neurological impairments. The proposed method has great potential to reduce the need for videofluoroscopic swallowing studies (the current gold standard method for swallowing assessment, which is invasive and nonportable) and to assist in the overall clinical assessment of swallowing sound signals.
Genomic Analysis of the Evolution and Global Spread of Hyper-invasive Meningococcal Lineage 5 (2015)

These data were inconsistent with a single point of origin followed by pandemic spread, rather suggesting that the sub-lineages had diversified and spread by asymptomatic transmission, with multiple distinct strains causing localised hyperendemic outbreaks.