gebhart/patrepeval huggingface dataset:
Role of Tissue Transglutaminase in Gliadin Binding to Reticular Extracellular Matrix and Relation to Coeliac Disease Autoantibodies (1998)

On different tissue sections, binding of gliadin to reticular matrix components was observed which was Ca2+-dependent, inhibited by Cu2+ and Zn2+ ions, by putrescine, and by preincubation with antibodies against tissue transglutaminase (tTG) suggesting that binding of gliadin is mediated by tTG. tTG was able to bind to gliadin and fibronectin fixed to microplates. Furthermore, tTG mediated binding of gliadin to fibronectin coated to microplates. On tissue sections, treatment with sera containing coeliac disease autoantibodies yielded staining patterns very similar to that of bound gliadin. Dual label experiments by means of conventional and laser scanning microscopy revealed that most of autoantibody binding sites are matched by bound gliadin. However, lack of competition between gliadin and autoantibody binding hints to ligands in very close vicinity of this enzyme. Furthermore, there were several autoantibody binding regions which did not bind gliadin. This implies the existence of further autoantigenic epitopes not related to tTG.
Metal Complexes Containing Multidentate Ligands (II). Dichloro and Dinitro Coblat (III) Complexes of 1,10-Dipropyl-5R-methyltriethylenetetraamine (1978)

A straight chain flexible tetraamine ligand, 1,l0-dipropyl-5R-methyltriethylenetetraamine(R-metrien) has been synthesized, and the dichloro and dinitro cobalt(III) complexes of this ligand have been prepared. The ligand has been expected to show a topological preference for the trans isomer. As expected, only trans geometrical isomers have been obtained and their structures have been elucidated via elemental analyses, nmr, electronic absorption and CD data.
Sarcopenia During Androgen-Deprivation Therapy for Prostate Cancer (2012)

In men receiving ADT, LBM decreased significantly after 12, 24, and 36 months.
Properties of carboxymethylated crosslinked hemoglobin A (1987)

The selective carboxymethylation of the N-terminal amino groups of hemoglobin A with glyoxylic acid and sodium cyanoborohydride has been studied as a function of the state of ligation of hemoglobin. The N-terminal residues have been established as the primary sites of reaction by peptide mapping of the tryptic digest of each chain and subsequent amino acid analysis of the modified peptides. With oxyhemoglobin, the desired derivatives with a carboxymethyl group at the N-terminal of either or both chains amounted to 55% [Di Donato, A., Fantl, W. J., Acharya, A. S., & Manning, J. M. (1983) J. Biol. Chem. 258, 11890-11895]. In the present study it is shown that with deoxyhemoglobin the amount of the desired derivative is increased to 75%. The oxygen equilibrium curve of hemoglobin A carboxymethylated on its four N-terminal residues [0.5 mM as tetramer in 50 mM [bis(2-hydroxyethyl)amino]tris(hydroxymethyl)methane (Bis-Tris), pH 7.5, 37 degrees C] had a P50 value of 30 mmHg (Hill coefficient n = 2.8, alkaline Bohr value = 0.4) compared to a P50 of 9 mmHg for unmodified hemoglobin under the same conditions (n = 2.5, alkaline Bohr value = 0.5). In carboxymethylated oxyhemoglobin A, cross-linked with the mild agent glycolaldehyde for 3.5 h, there was 85% of Mr 64,000 species and 15% of Mr 128,000 or higher species. For the former, the extent of cross-linking between two subunits was 19%. For the latter, there was 29% of two cross-linked subunits and 13% of three cross-linked subunits. Termination of cross-linking, which may be desirable in some circumstances, can be successfully achieved with isonicotinic acid hydrazide.(ABSTRACT TRUNCATED AT 250 WORDS)
Studies on Application of Ion Beam Breeding to Industrial Microorganisms at TIARA (2019)

Mutation-breeding technologies are useful tools for the development of new biological resources in plants and microorganisms. In Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) at the National Institutes for Quantum and Radiological Science and Technology, Japan, ion beams were explored as novel mutagens. The mutagenic effects of various ion beams on eukaryotic and prokaryotic microorganisms were described and their application in breeding technology for industrial microorganisms were discussed. Generally, the relative biological effectiveness (RBE) depended on the liner energy transfer (LET) and the highest RBE values were obtained with 12C5+ ion beams. The highest mutation frequencies were obtained at radiation doses that gave 1%–10% of surviving fraction. By using 12C5+ ion beams in this dose range, many microorganisms have been improved successfully at TIARA. Therefore, ion-beam breeding technology for microorganisms will have applications in many industries, including stable food production, sustainable agriculture, environmental conservation, and development of energy resources in the near future. Moreover, genome analyses of the ion-beam-induced mutants are in progress to clear the differences of mutational functions induced by different LET radiations in microorganisms. Further characterizations of mutations induced by different LET radiations will facilitate more effective use of ion beams in microorganisms breeding.
Wideband precision two-axis beam steerer tracking servo design and test results (1993)

A compact, light-weight, two-axis beam steerer (TABS) with a 38 mm (1.5') Beryllium clear aperture was designed into a 1000 Hz servo loop for precision tracking of a remote laser source. The design is composed of a quadrant photodiode, a track-tone demodulator, a linear ratiometric processor with sum and difference circuits, and a proportional/integral controller with a phase lead compensator. The demodulator is a phase-lock-loop used for locking onto and then synchronously detecting the envelope of a modulated tracking beacon. This approach provides background rejection, star discrimination (or any other CW source), receiver AC coupling, and 1/f noise reduction. The linear ratiometric processor provides x and y error signals to the controller which are independent of input signal levels. The far-field performance goal is < 0.5 microradians rms from a Landsat-type of base motion disturbance.
AMINO ACID SEQUENCE HOMOLOGY BETWEEN A SERINE PROTEASE INHIBITOR FROM BARLEY AND POTATO INHIBITOR I (1981)

Conference Abstract| April 01 1981 AMINO ACID SEQUENCE HOMOLOGY BETWEEN A SERINE PROTEASE INHIBITOR FROM BARLEY AND POTATO INHIBITOR I I. Svendsen; I. Svendsen 1Carlsberg Laboratory, Department of Chemistry and Carlsberg Research Laboratory; Department of Biotechnology, DK-2500 Copenhagen, Valby Search for other works by this author on: This Site PubMed Google Scholar I. Jonassen; I. Jonassen 1Carlsberg Laboratory, Department of Chemistry and Carlsberg Research Laboratory; Department of Biotechnology, DK-2500 Copenhagen, Valby Search for other works by this author on: This Site PubMed Google Scholar J. Hejgaard; J. Hejgaard 2Department of Biochemistry and Nutrition, Technical University of Denmark, DK-2800 Lyngby Search for other works by this author on: This Site PubMed Google Scholar S. Boisen S. Boisen 2Department of Biochemistry and Nutrition, Technical University of Denmark, DK-2800 Lyngby Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1981) 9 (2): 265P. https://doi.org/10.1042/bst009265pb Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation I. Svendsen, I. Jonassen, J. Hejgaard, S. Boisen; AMINO ACID SEQUENCE HOMOLOGY BETWEEN A SERINE PROTEASE INHIBITOR FROM BARLEY AND POTATO INHIBITOR I. Biochem Soc Trans 1 April 1981; 9 (2): 265P. doi: https://doi.org/10.1042/bst009265pb Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1981 Biochemical Society1981 Article PDF first page preview Close Modal You do not currently have access to this content.
Synthesis of porphyrins tailored with eight facially-encumbering groups. An approach to solid-state light-harvesting complexes (1994)

Synthetic models of the photosynthetic antenna complexes must achieve long-range 3-dimensional order encompassing a large number of porphyrinic pigments with limited direct contact of the pigments. In order to develop solid-state antenna complexes, we have synthesized porphyrins bearing benzyloxy groups projecting over both faces and optionally also around the periphery of the porphyrin. Routes have been established for prefunctionalizing benzaldehydes with various benzyloxy groups. Reaction of 2,6-bis, 3,5-bis, or 2,4,6-tris-(benzyloxy)benzaldehydes with pyrrole via the room temperature two-step one-flask porphyrin reaction provides direct access to the facially-encumbered porphyrins. The benzyloxybenzaldehydes react as efficiently as methoxybenzaldehydes, indicating the utility of the -OCH2- unit for introducing large substituents near the face of the porphyrin. The octakis and dodecakis(benzyloxy)porphyrins exhibit characteristic porphyrin absorption and fluorescence properties in solution. The crystal structure of meso-tetrakis[2,6-bis(2,3,4,5,6-pentafluorobenzyloxy)phenyl]porphyrin has been determined. The pentafluorobenzyloxy substituents provide a cavity on each side of the porphyrin plane which has an approximate cylindrical shape with a diameter of ~̊7.5 Å and a height of ≥ 4.5 Å. The porphyrin core parameters are those obtained for free base derivatives in which the inner hydrogen atoms are ordered. Crystal data: a = 14.759 (1) Å, b = 25.519 (2) Å, c = 13.100 (1) Å, α = 100.04 (1), β = 99.83 (1), γ = 88.25 (1), V = 4767.3 (6) Å3, all measurements at 127 K, triclinic, space group P 1, Z = 2 R1(F) = 0.097, for 10020 “observed” data, and wR2(F2) = 0.275 for 17761 total unique (all) data.
THE EFFECT OF SUBSTITUENTS ON THE HYDROGEN BONDING OF ADENINE AND URACIL DERIVATIVES (1967)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Electroactive polymer actuators with carbon aerogel electrodes (2011)

Electromechanically active polymers (EAPs) change their shape in response to the voltage stimulus. They can be used as actuators or sensors. Several researchers have introduced different materials and assembling methods for EAPs. It is generally accepted that the porous structure of electrodes with possibly high specific surface area can greatly improve the actuation performance. In this paper, carbon aerogel is introduced as a new material for fabrication of nanoporous electrodes for EAP actuators. Using the direct assembly process (DAP), carbon–ionic liquid EAPs with either activated or non-activated carbon aerogel electrodes are prepared and analyzed. Their electrochemical and electromechanical characteristics are presented and compared to our recently reported actuators based on carbide-derived carbon and activated carbon electrodes. The results show that our newly developed actuators with activated carbon aerogel electrodes and non-activated carbon aerogel electrodes have maximum strains of 1.2% and 1.3%, respectively, which are comparable to the parameters reported previously for carbide-derived carbon and activated carbon based actuators and also to any other low-voltage driven EAP actuators.
On solving 2D and 3D puzzles using curve matching (2005)

We approach the problem of 2D and 3D puzzle solving by matching the geometric features of puzzle pieces three at a time. First, we define an affinity measure for a pair of pieces in two stages, one based on a coarse-scale representation of curves and one based on a fine-scale elastic curve matching method. This re-examination of the top coarse-scale matches at the fine scale results in an optimal relative pose as well as a matching cost which is used as the affinity measure for a pair of pieces. Pairings with overlapping boundaries are impossible and are removed from further consideration, resulting in a set of top valid candidate pairs. Second, triples arising from generic junctions are formed from this rank-ordered list of pairs. The puzzle is solved by a recursive grouping of triples using a best-first search strategy, with backtracking in the case of overlapping pieces. We also generalize aspects of this approach to matching of 3D pieces. Specifically, ridges of 3D fragments scanned using a laser range finder are detected using a dynamic programming method. A pair of ridges are matched using a generalization of the 2D curve matching approach to space curves by using an energy solution involving curvature and torsion, which are computed using a novel robust numerical method. The reconstruction of map fragments and broken tiles using this method is illustrated.
Incidence of response and long-term follow-up in patients with hairy cell leukemia treated with recombinant interferon alfa-2a [see comments] (1990)

Thirty-five evaluable patients with hairy cell leukemia (HCL) were treated with recombinant interferon alfa-2a (rIFN-alpha 2a), given at a dose of 3 X 10(6) units (U) intramuscularly (IM) daily for 6 months followed by 3 X 10(6) U IM three times a week for an additional 18 months in a single institution study. All treatment was stopped after 24 months. Sixty-nine percent of patients achieved a partial response, 11% a minor response, and 3% (one patient) had stable disease. Six patients (17%) did not respond to rIFN-alpha 2a. Two patients (6%) achieved a response but later progressed on treatment. A total of 23 patients completed 2 years of treatment and are evaluable for long-term follow-up at a median of 20 months postcompletion of therapy (range 9 to 32 months). Eleven patients (48%) have had progression of their disease at a median of 10 months (range .5 to 25 months) after treatment was discontinued. Statistical analysis of pretreatment patient characteristics did not reveal any factor(s) associated with a high probability of responding to rIFN-alpha 2a; however, analysis of post-treatment variables measured after 2 years of treatment suggested that a low platelet count was associated with a high rate of disease progression. These findings are compared with other published trials using rIFN-alpha 2b, a similar but not identical rIFN preparation. We conclude that while rIFN-alpha 2a has a high overall response incidence, the rate of disease progression after therapy is discontinued approaches 50%, and that a subset of patients can be identified who are at high risk for recurrence after completing 2 years of treatment.
Sensitivity of steroid enzyme immunoassays. Comparison of four label enzymes in an assay system using a monoclonal anti-steroid antibody. (1989)

The sensitivities of monoclonal antibody-based enzyme immunoassays for 11-deoxycortisol using alkaline phosphatase (AP), horseradish peroxidase (HRP), beta-galactosidase (beta-GAL) and glucose oxidase (GOD) as labels were compared. The anti-11-deoxycortisol antibody used was that produced in ascites by inoculating antibody-secreting hybridoma cells into mice. Enzyme labeling of 11-deoxycortisol was carried out by the N-succinimidyl ester method. The activated ester of 4-(2-carboxyethylthio)-11-deoxycortisol was treated with each enzyme to give a homologous enzyme-labeled antigen. In the competitive immunoassay, the bound and free enzyme-labeled antigens were separated by a double antibody method and the enzymic activity of the immune precipitate was determined by colorimetric and fluorimetric methods. The AP activity was measured in three ways, using p-nitrophenyl phosphate, nicotinamide adenine dinucleotide phosphate (NADP), and 4-methylumbelliferyl phosphate as substrates. o-Nitrophenyl beta-D-galactopyranoside and 4-methylumbelliferyl beta-D-galactopyranoside were used for beta-GAL, and 3,3',5,5'-tetramethylbenzidine (TMB) and 3-(p-hydroxyphenyl)propionic acid (HPPA) for HRP. In the case of GOD, TMB and HPPA were used in combination with HRP. A dose-response curve with a high sensitivity was obtained in each 11-deoxycortisol assay system by the use of a minimum amount of the enzyme-labeled antigen at an appropriate dilution of monoclonal anti-11-deoxycortisol antibody (Ka = 2 x 10(10) M-1). The amounts of 11-deoxycortisol needed to displace 50% of the bound label ranged from 5 to 15 pg in the colorimetric methods, and 4-9 pg in the fluorimetric methods.(ABSTRACT TRUNCATED AT 250 WORDS)
Aquaporin-3-mediated hydrogen peroxide transport is required for NF-κB signalling in keratinocytes and development of psoriasis (2015)

Aquaporin 3 (AQP3), a water/glycerol channel protein, has been found to transport hydrogen peroxide (H2O2). Here, we show that H2O2, imported via AQP3, is involved in nuclear factor-κB (NF-κB) signalling in keratinocytes and in the pathogenesis of psoriasis. IL-23-mediated induction of psoriasis is reduced in AQP3 knockout mice (AQP3(-/-)), and is accompanied by impaired NF-κB activation and intracellular H2O2 accumulation. In primary keratinocyte cultures, cellular import of H2O2 produced by membrane NADPH oxidase 2 (Nox2) in response to TNF-α is facilitated by AQP3 and required for NF-κB activation by regulation of protein phosphatase 2A. As AQP3 associates with Nox2, we propose that this interplay constitutes H2O2-mediated signalling in response to TNF-α stimulation. Collectively, these data indicate that AQP3-facilitated H2O2 transport is required for NF-κB activation in keratinocytes in the development of psoriasis.
Learning to boost GMM based speaker verification (2003)

The Gaussian mixture models (GMM) has proved to be an effective probabilistic model for speaker verification, and has been widely used in most of state-of-the-art systems. In this paper, we introduce a new method for the task: that using AdaBoost learning based on the GMM. The motivation is the following: While a GMM linearly combines a number of Gaussian models according to a set of mixing weights, we believe that there exists a better means of combining individual Gaussian mixture models. The proposed AdaBoost-GMM method is non-parametric in which a selected set of weak classifiers, each constructed based on a single Gaussian model, is optimally combined to form a strong classifier, the optimality being in the sense of maximum margin. Experiments show that the boosted GMM classifier yields 10.81% relative reduction in equal error rate for the same handsets and 11.24% for different handsets, a significant improvement over the baseline adapted GMM system.
Idiopathic intracranial hypertension: mechanisms of visual loss and disease management. (2000)

Idiopathic intracranial hypertension (IIH) is a disorder of increased intracranial pressure of unknown cause. It is a disorder, predominantly of overweight women in the childbearing years. The major morbidity of the disease is visual loss. Damage to the visual system occurs at the optic nerve head. This damage is most likely due to axoplasm flow stasis and resultant intraneuronal ischemia. Management of IIH begins with educating the patient about the disease and its potential outcomes. I recommend modest dieting and following a low-salt regimen with caution against overuse of fluids. Acetazolamide and Lasix appear to be efficacious. Patients failing medical therapy have optic nerve sheath fenestration performed if visual loss is the main morbidity. Shunting procedures are considered if headache is the main symptom. Most patients respond well to therapy, but idiopathic intracranial hypertension may recur throughout life.
Abstract 1131: Novel inhibitor of Notch signaling for the treatment of cancer. (2013)

Abstract The Notch pathway is a highly conserved signaling system that plays an important role in development and tissue homeostasis. While Notch mutations are well characterized and implicated in hematological malignancies such as T-cell acute lymphoblastic leukemia, mutations in solid tumors were not reported until recently. With the whole genomic deep sequencing of a large number of samples, deregulated Notch signaling has been implicated in a small percentage of solid tumors such as ovarian, lung, and triple negative breast cancer due to genomic alterations including mutations, amplification, and fusion of Notch pathway components. Inhibition of Notch signaling may provide an attractive targeted cancer therapeutic strategy. We have identified and characterized LY3039478 a novel small molecule that is an exquisitely potent inhibitor of Notch-1 intracellular domain (N1ICD) cleavage with an IC50 of ∼1nM in most of the tumor cell lines tested. We also demonstrate that LY3039478 potently inhibits mutant Notch receptor activity. In a xenograft tumor model, LY3039478 inhibited N1ICD cleavage and expression of Notch-regulated genes in the tumor microenvironment. The inhibition of Notch cleavage also resulted in the induction of apoptosis in a Notch-dependent xenograft model. Using extensive PK/PD data we determined the strength and duration of N1ICD cleavage required for anti-tumor activity which was observed in several xenograft tumors including patient derived tumors representing colon, lung, ovarian, gastric, and breast cancer and glioblastoma. To mitigate the mucoid gasteroentropathy caused by Notch inhibition, PK/PD data were incorporated in devising dosing strategies that identified an optimal intermittent dosing schedule without negatively impacting efficacy. Furthermore, the mucoid gastroentropathy was also mitigated by the prophylactic administration of dexamethasone without negatively impacting the Notch inhibitor mediated efficacy. Mechanistic studies revealed that dexamethasone does not interfere with LY3039478-mediated inhibition of N1ICD cleavage and gene expression but alters the expression of stem cell gene expression in GI tract. In summary, we have characterized an orally bio-available small molecule Notch inhibitor that may provide therapeutic benefit to cancer patients with deregulated Notch signaling. LY3039478 is specifically designed to potently inhibit Notch signaling and is being investigated in Phase I. Citation Format: Mark H. Bender, Hong Gao, Andrew R. Capen, Julia M. Clay, Philip A. Hipskind, Jon K. Reel, Maciej J. Zamek-Gliszczynski, Jason R. Manro, Karim Benhadji, Bharvin K. R. Patel. Novel inhibitor of Notch signaling for the treatment of cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1131. doi:10.1158/1538-7445.AM2013-1131
ChemInform Abstract: HETEROGENEOUS HALIDE‐SILICA PHOSPHORS (1975)

Abstract Photolumineszenzuntersuchungen werden an Phosphoren aus 90% SiO 2 und 10% CaCl 2 (oder anderen Halogeniden), aktiviert mit Eu(2+) (violett bis blau) oder mit Eu(2+) + Mn(2+) (gelb bis rot), durchgeführt.