gebhart/patrepeval huggingface dataset:
A pilot study on the effects of music therapy on frontotemporal dementia – developing a research protocol1 (2009)

Some forms of dementia particularly affect the frontal parts of the brain which, in some cases, causes the onset of severe behavioural and psychological symptoms. No specific treatment for the primary diseases that cause these frontotemporal dementia conditions has yet been developed, and pharmacological treatment of the psychiatric symptoms is difficult, requiring specialist proficiency in the field. As there is not yet sufficient research that examines the effects of non-pharmacologic treatment with this group, there is a need to develop valid and reliable research protocols. Music therapy was investigated as an example of a non-pharmacologic treatment procedure. A pilot study was carried out with the focus to develop a research protocol for a future larger population study. In two case studies a combination of data collection methods were examined with the overall goal to document changes in intersubjectivity. In this pilot study there was a specific interest in selecting a relevant and manageable dementia specific instrument for measuring quality of life and relating it with other instruments. The following three instruments were tested: the Alzheimer's Disease-Related Quality of Life (ADRQL), the Cohen–Mansfield Agitation Inventory (CMAI), and the Neuro-psychiatric Inventory (NPI), and were related to case descriptions and video analyses. Recommendations for a mixed method research protocol focused on measuring the effect of music therapy with persons with frontotemporal dementia are presented.
Adoption of business continuity planning processes in IT service management (2008)

For any fault of the same severity level, traditional fault discovery and notification tools provide equal weighting from business points of view. To improve the fault correlation from business perspectives, we proposed a framework to automate network and system alerts with respect to its business service impact for proactive notification to IT operations management. This paper outlines the value of business continuity planning (BCP) during the course of service impact analysis, placing particular emphasis on the business perspective in the processes of IT service management. The framework explicitly employs BCP relevant processes in order to identify the relationships between business services and IT resources A practical case in IT operations to illustrate the concept was then conducted.
Kokumi Substances, Enhancers of Basic Tastes, Induce Responses in Calcium-Sensing Receptor Expressing Taste Cells (2012)

Recently, we reported that calcium-sensing receptor (CaSR) is a receptor for kokumi substances, which enhance the intensities of salty, sweet and umami tastes. Furthermore, we found that several γ-glutamyl peptides, which are CaSR agonists, are kokumi substances. In this study, we elucidated the receptor cells for kokumi substances, and their physiological properties. For this purpose, we used Calcium Green-1 loaded mouse taste cells in lingual tissue slices and confocal microscopy. Kokumi substances, applied focally around taste pores, induced an increase in the intracellular Ca(2+) concentration ([Ca(2+)](i)) in a subset of taste cells. These responses were inhibited by pretreatment with the CaSR inhibitor, NPS2143. However, the kokumi substance-induced responses did not require extracellular Ca(2+). CaSR-expressing taste cells are a different subset of cells from the T1R3-expressing umami or sweet taste receptor cells. These observations indicate that CaSR-expressing taste cells are the primary detectors of kokumi substances, and that they are an independent population from the influenced basic taste receptor cells, at least in the case of sweet and umami.
Fusion between human mesenchymal stem cells and rodent cerebellar Purkinje cells (2010)

we believe that this is the first paper to define fusion and heterokaryon formation between human MSCs and rodent cerebellar neurons in vivo. We have also demonstrated that fusion between these cell populations occurs in vitro. These findings indicate that MSCs may be potential therapeutic agents for cerebellar diseases, and other neuroinflammatory and neurodegenerative disorders.
Nursery Technology of Spruce ( Picea smithiana ) (1986)

Distribution and importance of Spruce ( Picea smithiana ) and silver fir ( Abies pindrow ) forests of Western Himalayas has been discussed. Regeneration problem of the spruce has been high-lighted. Seed collection, control against damping off and nursery technology of spruce has been discussed in details.
Distributed Kalman Filter with Embedded Consensus Filters (2006)

The problem of distributed Kalman filtering (DKF) for sensor networks is one of the most fundamental distributed estimation problems for scalable sensor fusion. This paper addresses the DKF problem by reducing it to two separate dynamic consensus problems in terms of weighted measurements and inverse-covariance matrices. These to data fusion problems are solved is a distributed way using low-pass and band-pass consensus filters. Consensus filters are distributed algorithms that allow calculation of average-consensus of time-varying signals. The stability properties of consensus filters is discussed in a companion CDC ’05 paper [24]. We show that a central Kalman filter for sensor networks can be decomposed into n micro-Kalman filters with inputs that are provided by two types of consensus filters. This network of micro-Kalman filters collectively are capable to provide an estimate of the state of the process (under observation) that is identical to the estimate obtained by a central Kalman filter given that all nodes agree on two central sums. Later, we demonstrate that our consensus filters can approximate these sums and that gives an approximate distributed Kalman filtering algorithm. A detailed account of the computational and communication architecture of the algorithm is provided. Simulation results are presented for a sensor network with 200 nodes and more than 1000 links.
Effect of furosemide on physiologic variables in exercising horses. (1993)

Twelve horses (6 Standardbreds and 6 Thoroughbreds) received IM injections of furosemide (250 mg) or physiologic saline solution and performed standard exercise tests, to assess the effects of furosemide and breed on blood gas values, PCV, plasma lactate concentration, and heart rate during exercise. After furosemide administration, arterial and venous blood pH values were significantly (P < 0.05) increased. Partial pressures of O2 and CO2 in arterial blood and of CO2 in venous blood (PaO2, PaCO2, and PVCO2, respectively) were unaffected by furosemide treatment, whereas venous partial pressures of O2 (PVO2) were significantly (P < 0.05) less during exercise after furosemide treatment, suggesting an increase in oxygen uptake by the exercising muscles or a change in cardiac output. A significant (P < 0.05) difference was found between Thoroughbred and Standardbred values for arterial and venous pH, PaO2, PaCO2, plasma lactate concentration, and heart rate, suggesting that Standardbreds exercised at a relatively higher work rate than did Thoroughbreds.
Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica (2019)

Recombinant protein production represents a multibillion-dollar market. Therefore, it constitutes an important research field both in academia and industry. The use of yeast as a cell factory presents several advantages such as ease of genetic manipulation, growth at high cell density, and the possibility of post-translational modifications. <i>Yarrowia lipolytica</i> is considered as one of the most attractive hosts due to its ability to metabolize raw substrate, to express genes at a high level, and to secrete protein in large amounts. In recent years, several reviews have been dedicated to genetic tools developed for this purpose. Though the construction of efficient cell factories for recombinant protein synthesis is important, the development of an efficient process for recombinant protein production in a bioreactor constitutes an equally vital aspect. Indeed, a sports car cannot drive fast on a gravel road. The aim of this review is to provide a comprehensive snapshot of process tools to consider for recombinant protein production in bioreactor using <i>Y. lipolytica</i> as a cell factory, in order to facilitate the decision-making for future strain and process engineering.
Oral Effectiveness of l-Thyroxine, l-Triiodothyronine, and Thyroprotein as Compared to Injections of l-Thyroxine and Thyroprotein (1965)

The thyroid hormone secretion rate (TSR) of ten nonlactating dairy cows was determined using L-thyroxine (L-T~) and thyroprotein by injection. The equivalent biological effectiveness of L-T, L-triiodothyronine (L-T~), and thyroprotein by oral administration was then determined.
18 GHz low-power CMOS static frequency divider (2003)

A pseudo-differential latch circuit is investigated. By removing the current source from the conventional source-coupled field-effect-transistor logic (SCFL) structure, the speed of the circuit can be improved. The pseudo-differential D-type flip-flop-based 2:1 static frequency divider, which can operate up to 18 GHz and consumes less than 4 mA from a 1.8 V supply, has been realised in 0.18 µm standard digital CMOS technology.
Functional Analysis of Fructosyl-Amino Acid Oxidases of <i>Aspergillus oryzae</i> (2004)

Three active fractions of fructosyl-amino acid oxidase (FAOD-Ao1, -Ao2a, and -Ao2b) were isolated from Aspergillus oryzae strain RIB40. N-terminal and internal amino acid sequences of FAOD-Ao2a corresponded to those of FAOD-Ao2b, suggesting that these two isozymes were derived from the same protein. FAOD-Ao1 and -Ao2 were different in substrate specificity and subunit assembly; FAOD-Ao2 was active toward N(epsilon)-fructosyl N(alpha)-Z-lysine and fructosyl valine (Fru-Val), whereas FAOD-Ao1 was not active toward Fru-Val. The genes encoding the FAOD isozymes (i.e., FAOAo1 and FAOAo2) were cloned by PCR with an FAOD-specific primer set. The deduced amino acid sequences revealed that FAOD-Ao1 was 50% identical to FAOD-Ao2, and each isozyme had a peroxisome-targeting signal-1, indicating their localization in peroxisomes. The genes was expressed in Escherichia coli and rFaoAo2 showed the same characteristics as FAOD-Ao2, whereas rFaoAo1 was not active. FAOAo2 disruptant was obtained by using ptrA as a selective marker. Wild-type strain grew on the medium containing Fru-Val as the sole carbon and nitrogen sources, but strain Delta faoAo2 did not grow. Addition of glucose or (NH(4))(2)SO(4) to the Fru-Val medium did not affect the assimilation of Fru-Val by wild-type, indicating glucose and ammonium repressions did not occur in the expression of the FAOAo2 gene. Furthermore, conidia of the wild-type strain did not germinate on the medium containing Fru-Val and NaNO(2) as the sole carbon and nitrogen sources, respectively, suggesting that Fru-Val may also repress gene expression of nitrite reductase. These results indicated that FAOD is needed for utilization of fructosyl-amino acids as nitrogen sources in A. oryzae.
Preparation of Polyacrylate Thin Films Containing Zirconia Nano-Particles and Their Optical Properties (2010)

ビニルトリメトキシシランと 2-メタクリロイルオキシエチルイソシアネートを表面処理剤として用いて，ビーズミル処理と超音波照射によるジルコニアナノ粒子の表面処理を行った．特に，超音波照射により，2-メタクリロイルオキシエチルイソシアネートとジルコニア表面のヒドロキシル基とのウレタン結合を形成することで有機化し，メチルエチルケトン中で粒子径 8.5 nm のジルコニアナノ粒子分散液を調製することができた．得られたジルコニアナノ粒子を高分散した多官能アクリレート溶液に光ラジカル開始剤を添加し，これらを PET フィルム上にバーコーターで塗布して薄膜作製した．この薄膜への紫外線照射によって光架橋したポリアクリレート薄膜においても，ナノ粒子が均一に分散している事がわかった．これらのジルコニアナノ粒子分散ポリアクリレート薄膜は，高屈折率を示した．
Integration of Utilities Infrastructures in a Future Internet Enabled Smart City Framework (2013)

Improving efficiency of city services and facilitating a more sustainable development of cities are the main drivers of the smart city concept. Information and Communication Technologies (ICT) play a crucial role in making cities smarter, more accessible and more open. In this paper we present a novel architecture exploiting major concepts from the Future Internet (FI) paradigm addressing the challenges that need to be overcome when creating smarter cities. This architecture takes advantage of both the critical communications infrastructures already in place and owned by the utilities as well as of the infrastructure belonging to the city municipalities to accelerate efficient provision of existing and new city services. The paper highlights how FI technologies create the necessary glue and logic that allows the integration of current vertical and isolated city services into a holistic solution, which enables a huge forward leap for the efficiency and sustainability of our cities. Moreover, the paper describes a real-world prototype, that instantiates the aforementioned architecture, deployed in one of the parks of the city of Santander providing an autonomous public street lighting adaptation service. This prototype is a showcase on how added-value services can be seamlessly created on top of the proposed architecture.
ALCOHOLICS WITH REDUCED DOPAMINE D<sub>2</sub> RECEPTOR FUNCTION POSSESS NORMAL PLATELET MONOAMINE OXIDASE ACTIVITY (1994)

Platelet monoamine oxidase (MAO) activity, proposed to be a marker for central 5-hydroxytryptamine (5-HT) capacity, was investigated in 14 severely alcohol-dependent subjects with reduced dopamine (DA) D2 receptor function, as assessed by the growth hormone responses to apomorphine. Twelve healthy men were used as controls. Platelet MAO activity in the alcohol-dependent subjects was not different from that in controls. The finding from this preliminary study suggests that severely alcohol-dependent subjects with reduced DA D2 receptor function have normal 5-HT capacity.
Myofiber integrity depends on desmin network targeting to Z-disks and costameres via distinct plectin isoforms (2008)

Dysfunction of plectin, a 500-kD cytolinker protein, leads to skin blistering and muscular dystrophy. Using conditional gene targeting in mice, we show that plectin deficiency results in progressive degenerative alterations in striated muscle, including aggregation and partial loss of intermediate filament (IF) networks, detachment of the contractile apparatus from the sarcolemma, profound changes in myofiber costameric cytoarchitecture, and decreased mitochondrial number and function. Analysis of newly generated plectin isoform-specific knockout mouse models revealed that IF aggregates accumulate in distinct cytoplasmic compartments, depending on which isoform is missing. Our data show that two major plectin isoforms expressed in muscle, plectin 1d and 1f, integrate fibers by specifically targeting and linking desmin IFs to Z-disks and costameres, whereas plectin 1b establishes a linkage to mitochondria. Furthermore, disruption of Z-disk and costamere linkages leads to the pathological condition of epidermolysis bullosa with muscular dystrophy. Our findings establish plectin as the major organizer of desmin IFs in myofibers and provide new insights into plectin- and desmin-related muscular dystrophies.
Statistical Analysis and Empirical Study for Life Insurance (2009)

According to the uncertainty of insurance accident, in this article, we introduce the importance of insurance and the application of probability statistics in the insurance, briefly describe several parameter models about life distribution (i.e. life models), discuses the establishment and use of the life table, and statistically analyze the profit and loss and insured amount in life insurance. The result could possess certain directional meanings and reference function for practical works.
PCR Detection of Microbial Pathogens (2002)

Part I. Reviews Specificity and Performance of Diagnostic PCR Assays Konrad Sachse Pre-PCR Processing of Samples Peter Radstrom, Rickard Knutsson, Petra Wolffs, Maria Dahlenborg, and Charlotta Lofstrom Critical Aspects of Standardization of PCR Jeffrey Hoorfar and Nigel Cook PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens Peter Stephensen Lubeck and Jeffrey Hoorfar Part II. Protocols Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae Joachim Frey Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR Darla R. Ewalt and Betsy J. Bricker Isolation of Campylobacter and Identification by PCR Mark D. Englen, Scott R. Ladely, and Paula J. Fedorka-Cray Detection and Differentiation of Chlamydiae by Nested PCR Konrad Sachse and Helmut Hotzel Detection of Toxigenic Clostridia Michel R. Popoff PCR-Based Detection of Coxiella burnetii from Clinical Samples Mustapha Berri, Nathalie Arricau-Bouvery, and Annie Rodolakis Detection and Subtyping of Shiga Toxin-Producing Escherichia coli (STEC) Peter Gallien Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay Louise O'Connor Detection of Leptospira interrogans John W. Lester and Rance B. LeFebvre Detection of Pathogenic Mycobacteria of Veterinary Importance Robin A. Skuce, M. Siobhan Hughes, Malcolm J. Taylor, and Sydney D. Neill Multiplex PCR of Avian Pathogenic Mycoplasmas Mazhar I. Khan Detection and Differentiation of Ruminant Mycoplasmas Helmut Hotzel, Joachim Frey, John Bashiruddin, and Konrad Sachse Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air Marylene Kobisch and Joachim Frey PCR-Detection of Hemophilus paragallinarum, Hemophilus somnus, Mannheimia (Pasteurella) hemolytica, Mannheimia spp., Pasteurella trehalosi, and Pasteurella multocida Henrik Christensen, Magne Bisgaard, Jesper Larsen, and John Elmerdahl Olsen Detection of Salmonella spp. Burkhard Malorny and Reiner Helmuth Detection of Toxoplasma gondii Jonathan M. Wastling and Jens G. Mattsson PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples Edoardo Pozio and Giuseppe La Rosa Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure Rickard Knutsson and Peter Radstrom Index
Effect of expression of human spermidine/spermine N1-acetyltransferase in Escherichia coli (1995)

A plasmid expression vector, pINSAT2, was constructed in order to express spermidine/spermine N1-acetyltransferase (SSAT) in Escherichia coli. Cells transfected with this vector produced large amounts of SSAT, amounting to up to 2% of the soluble protein when isopropyl beta-D-thiogalactopyranoside (IPTG) was added and 0.3% of the soluble protein in the absence of inducer. The growth rate of cells expressing SSAT was reduced, and all of the cellular spermidine was converted to N1-acetylspermidine, much of which was excreted. Putrescine and 1-methylspermidine, which is not a substrate for SSAT, could reverse the effects of SSAT expression on growth, but spermidine was only effective when the amount of SSAT expression was limited by omitting the IPTG inducer. The lack of stimulation of growth by spermidine correlated with its complete conversion to N1-acetylspermidine. These results show that N1-acetylspermine is not able to substitute for the unmodified polyamines in supporting growth and suggest that acetylation is a physiological response to convert excess polyamines to a physiologically inert form which is readily excreted. Cells expressing large amounts of SSAT were much more sensitive to the growth inhibitory action of the antitumor agent N1,N12-bis(ethyl)spermine, supporting the hypothesis that the ability of such bis(ethyl) polyamines to induce SSAT contributes to their antiproliferative actions. SSAT was readily purified to homogeneity from extracts of DH5 alpha cells containing pINSAT2. The purified enzyme had a similar specific activity and Km values for spermine and spermidine as the enzyme purified from human colon cancer cells, suggesting that posttranslational modifications specific to eukaryotes are not needed for enzymatic activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Epitope Mapping and Competitive Binding of HSA Drug Site II Ligands by NMR Diffusion Measurements (2004)

It is important to characterize drug-albumin binding during drug discovery and lead optimization as strong binding may reduce bioavailability and/or increase the drug's in vivo half-life. Despite knowing about the location of human serum albumin (HSA) drug binding sites and the residues important for binding, less is understood about the binding dynamics between exogenous drugs and endogenous fatty acids. In contrast to highly specific antibody-antigen interactions, the conformational flexibility of albumin allows the protein to adopt multiple conformations of approximately equal energy in order to accommodate a variety of ligands. Nuclear magnetic resonance (NMR) diffusion measurements are a simple way to quantitatively describe ligand-protein interactions without prior knowledge of the number of binding sites or the binding stoichiometry. This method can also provide information about ligand orientation at the binding site due to buildup of exchange-transferred NOE (trNOE) on the diffusion time scale of the experiment. The results of NMR diffusion and NOE experiments reveal multiple binding interactions of HSA with dansylglycine, a drug site II probe, and caprylate, a medium-chain fatty acid that also has primary affinity for HSA's drug site II. Interligand NOE (ilNOE) detected in the diffusion analysis of a protein solution containing both ligands provides insight into the conformations adopted by these ligands while bound in common HSA binding pockets. The results demonstrate the ability of NMR diffusion experiments to identify ternary complex formation and show the potential of this method for characterizing other biologically important ternary structures, such as enzyme-cofactor-inhibitor complexes.
Ignition of Ethylene–Air and Methane–Air Flows by Low-Temperature Repetitively Pulsed Nanosecond Discharge Plasma (2007)

This paper presents results of low-temperature plasma-assisted combustion experiments in premixed ethylene-air and methane-air flows. The plasma was generated by high-voltage, nanosecond pulse duration, high repetition rate pulses. The high reduced electric field during the pulse allows efficient electronic excitation and molecular dissociation, thereby generating a pool of chemically active radical species. The low duty cycle of the repetitively pulsed discharge improves the discharge stability and helps sustain diffuse, uniform, and volume filling nonequilibrium plasma. Plasma temperature was inferred from nitrogen second positive band system emission spectra and calibrated using thermocouple measurements in preheated flows (without plasma). The experiments showed that adding fuel to the air flow considerably increases the flow temperature in the plasma, up to DeltaT = 250degC-350degC. On the other hand, adding fuel to nitrogen flow at the same flow and discharge conditions resulted in a much less pronounced plasma temperature rise, only by about DeltaT = 50degC. This shows that temperature rise in the air-fuel plasma is due to plasma chemical fuel oxidation reactions initiated by the radicals generated in the plasma. In a wide range of conditions, generating the plasma in air-fuel flows resulted in flow ignition, flameholding, and steady combustion downstream of the discharge. Plasma-assisted ignition occurred at low air plasma temperatures, 100degC-200 degC, and low discharge powers, ~100 W (~1% of heat of reaction). At these conditions, the reacted fuel fraction is up to 85%-95%. The present results suggest that the flow temperature rise caused by plasma chemical fuel oxidation results in flow ignition downstream of the plasma.