gebhart/patrepeval huggingface dataset:
Crosslinked polyimide hollow fiber membranes for aggressive natural gas feed streams (2008)

Natural gas is one of the fastest growing primary energy sources in the world today. The increasing world demand for energy requires increased production of high quality natural gas. For the natural gas to be fed into the mainline gas transportation system, it must meet the pipe-line quality standards. Natural gas produced at the wellhead is usually "sub-quality" and contains various impurities such as CO2, H2S, and higher hydrocarbons, which must be removed to meet specifications.\n\nCarbon dioxide is usually the most abundant impurity in natural gas feeds and high CO2 partial pressures in the feed can lead to plasticization, which causes loss of some methane product and may ultimately render the membrane ineffective. Moreover, the presence of highly sorbing higher hydrocarbons in the feed can further reduce membrane performance. \n\nCovalent crosslinking has been shown to increase plasticization resistance in dense films by suppressing the degree of swelling and segmental chain mobility in the polymer, thereby preserving the selectivity of the membrane. This research focuses on extending the dense film success to asymmetric hollow fibers. \n\nIn this work, the effect of high pressure CO2 (up to 400 psia CO2 partial pressure) on CO2/CH4 mixed gas separation performance was investigated on defect-free the hollow fiber membrane at different degrees of crosslinking. All the crosslinked fibers were shown to exhibit good resistance to selectivity losses from CO2 induced plasticization, significantly more than the uncrosslinked fibers. Robust resistance of the hollow fiber membranes in the presence of toluene (a highly sorbing contaminant) was also demonstrated as the membranes showed no plasticization. Antiplasticization was found to occur in the presence of toluene feeds with the crosslinkable fibers used in this work.
Identification and Validation of Mannose 6-Phosphate Glycoproteins in Human Plasma Reveal a Wide Range of Lysosomal and Non-lysosomal Proteins (2006)

Acid hydrolase activities are normally confined within the cell to the lysosome, a membrane-delimited cytoplasmic organelle primarily responsible for the degradation of macromolecules. However, lysosomal proteins are also present in human plasma, and a proportion of these retain mannose 6-phosphate (Man-6-P), a modification on N-linked glycans that is recognized by Man-6-P receptors (MPRs) that normally direct the targeting of these proteins to the lysosome. In this study, we purified the Man-6-P glycoforms of proteins from human plasma by affinity chromatography on immobilized MPRs and characterized this subproteome by two-dimensional gel electrophoresis and by tandem mass spectrometry. As expected, we identified many known and potential candidate lysosomal proteins. In addition, we also identified a number of abundant classical plasma proteins that were retained even after two consecutive rounds of affinity purification. Given their abundance in plasma, we initially considered these proteins to be likely contaminants, but a mass spectrometric study of Man-6-phosphorylation sites using MPR-purified glycopeptides revealed that some proportion of these classical plasma proteins contained the Man-6-P modification. We propose that these glycoproteins are phosphorylated at low levels by the lysosomal enzyme phosphotransferase, but their high abundance results in detection of Man-6-P glycoforms in plasma. These results may provide useful insights into the molecular processes underlying Man-6-phosphorylation and highlight circumstances under which the presence of Man-6-P may not be indicative of lysosomal function. In addition, characterization of the plasma Man-6-P glycoproteome should facilitate development of mass spectrometry-based tools for the diagnosis of lysosomal storage diseases and for investigating the involvement of Man-6-P-containing glycoproteins in more widespread human diseases and their potential utility as biomarkers.
Adaptive noise reduction and image sharpening for digital video compression (2002)

This paper presents a framework of digital video preprocessing for digital video compression. The purpose of this preprocessing is to reduce the noise and thus improve the video compression efficiency. A four stage processing system has been proposed. The first process is a median filter to remove the 2D impulse noise. The second process is a 2D spatial filter that is controllable by the video encoder parameters. The third process is an image sharpening operation to enhance the image resolution or details so that the decoded video from the encoded bitstream still gives high resolution content. The last process is a recursive temporal filter that is used to reduce the temporal noise. The recursive filter is designed to adapt to the video content so that high quality temporal filtering is achieved.
Dynamic Interaction of a Layer and a Half-Space (1967)

The equations of the theory of linear elasticity are used to compute dispersion curves for free time-harmonic waves propagating in a layer and a supporting half-space of different material properties. Both welded and smooth contacts are considered, and the layer may be acoustically softer or stiffer than the half-space. An infinite number of modes exists if the velocity of shear waves in the layer is less than the velocity of shear waves in the half-space. Generally, only one mode exists in the opposite case. The phase velocities of the two lowest modes approach, with decreasing wave length, the velocities of Rayleigh waves in the layer and the velocity of Stoneley waves, if the latter waves are possible. The phase velocities of all higher modes, if these modes exist, approach the velocity of shear waves in the layer. The conditions for existence of Stoneley waves are established.
Fisher Exact Scanning for Dependency (2016)

We introduce a method---called Fisher exact scanning (FES)---for testing and identifying variable dependency that generalizes Fisher's exact test on $2\times 2$ contingency tables to $R\times C$ contingency tables and continuous sample spaces. FES proceeds through scanning over the sample space using windows in the form of $2\times 2$ tables of various sizes, and on each window completing a Fisher's exact test. Based on a factorization of Fisher's multivariate hypergeometric (MHG) likelihood into the product of the univariate hypergeometric likelihoods, we show that there exists a coarse-to-fine, sequential generative representation for the MHG model in the form of a Bayesian network, which in turn implies the mutual independence (up to deviation due to discreteness) among the Fisher's exact tests completed under FES. This allows an exact characterization of the joint null distribution of the $p$-values and gives rise to an effective inference recipe through simple multiple testing procedures such as Šidák and Bonferroni corrections, eliminating the need for resampling. In addition, FES can characterize dependency through reporting significant windows after multiple testing control. The computational complexity of FES is approximately linear in the sample size, which along with the avoidance of resampling makes it ideal for analyzing massive data sets. We use extensive numerical studies to illustrate the work of FES and compare it to several state-of-the-art methods for testing dependency in both statistical and computational performance. Finally, we apply FES to analyzing a microbiome data set and further investigate its relationship with other popular dependency metrics in that context.
Effects of Cations on the Performance of the Photoanode in the n ‐ GaAs |  K 2Se ‐  K 2Se2 ‐  KOH  |  C  Semiconductor Liquid Junction Solar Cell (1979)

Cations adsorbed on affect the fill factor and the open‐circuit voltage of the cell. An AM1 solar‐to‐electrical conversion efficiency of 12% is reached by chemisorbing Ru(III) on the photoanode. This efficiency is maintained upon passage of 35,000 C/cm2. The effect of Ru(III) is interpreted as a reaction with a surface chemical entity associated with a surface state near the conduction band, which shunts the cell by allowing electrons to tunnel through the barrier at the junction. Reaction with Ru(III) converts the shunting surface state to one to which electrons cannot tunnel. Being strongly adsorbed, Ru(III) also reduces the effect of certain impurities such as Bi(III) which otherwise decrease the power output.
Histochemical and physiological characteristics of the rat diaphragm (1985)

The histochemical and contractile characteristics of the adult rat diaphragm were determined. Based on enzyme histochemistry, the rat diaphragm contained 40% type I, 27% type IIa, and 34% type IIb fibers. There were significantly more type I fibers in the ventral costal (VEN) compared with the crural (CRU) region of the muscle and a slightly higher percentage of type I's on the thoracic relative to the abdominal surface. The contractile properties and the effect of temperature (Q10) were similar in the VEN and CRU regions. Increasing temperature produced higher isometric peak tetanic tension, whereas twitch tension, contraction, and one-half relaxation time all decreased. The maximal shortening velocity increased linearly from 22 and 30 degrees C, then plateaued before decreasing between 35 and 37 degrees C. The VEN and CRU force-velocity curves became less concave as temperature increased from 22 to 35 degrees C. Furthermore, the force-frequency relation of both regions was shifted to the right as temperature increased. The isometric and isotonic contractile properties and fiber type distribution are similar in the VEN and CRU regions of the diaphragm. The rat diaphragm is clearly heterogeneous in fiber type distribution and functionally lies intermediate between slow- and fast-twitch limb skeletal muscles.
Anodic oxidations. VI. p-Cyanation of diphenylamines (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnodic oxidations. VI. p-Cyanation of diphenylaminesKunihisa Yoshida and Takayuki FuenoCite this: J. Org. Chem. 1972, 37, 25, 4145–4147Publication Date (Print):December 1, 1972Publication History Published online1 May 2002Published inissue 1 December 1972https://pubs.acs.org/doi/10.1021/jo00798a039https://doi.org/10.1021/jo00798a039research-articleACS PublicationsRequest reuse permissionsArticle Views194Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
N-ethyl-N-nitrosourea-induced null mutation at the mouse Car-2 locus: an animal model for human carbonic anhydrase II deficiency syndrome. (1988)

Electrophoretic screening of (C57BL/6J x DBA/2J)F1 progeny of male mice treated with N-ethyl-N-nitrosourea revealed a mouse that lacked the paternal carbonic anhydrase II (CA II). Breeding tests showed that this trait was heritable and due to a null mutation at the Car-2 locus on chromosome 3. Like humans with the same inherited enzyme defect, animals homozygous for the new null allele are runted and have renal tubular acidosis. However, the prominent osteopetrosis found in humans with CA II deficiency could not be detected even in very old homozygous null mice. A molecular analysis of the deficient mice shows that the mutant gene is not deleted and is transcribed. The CA II protein, which is normally expressed in most tissues, could not be detected by immunodiffusion analysis in any tissues of the CA II-deficient mice, suggesting a nonsense or a missense mutation at the Car-2 locus.
Extracellular ATP Is Involved in the Induction of Apoptosis in Murine Hematopoietic Cells (2007)

Extracellular nucleotides have multiple biological actions in processes such as proliferation, differentiation, chemotaxis, and cytokine secretion through P2X receptors on the cell surface. To determine the biological activity of adenosine triphosphate (ATP) and the expression of P2 nucleotide receptors in murine bone marrow-derived hematopoietic cells and stem cells/progenitor cells, we investigated the effects of ATP in assays of cell proliferation and cell death in vitro. Our results demonstrated that several subtypes of P2X receptors were expressed on hematopoietic cells and that P2X7, in particular, was partially expressed in hematopoietic stem cells/progenitor cells. In addition, stimulation of hematopoietic cells with high concentrations of ATP caused severe inhibition of cell proliferation despite the presence of cytokine stimulation. We analyzed the apoptotic effects of stimulation with several different dosages of ATP and confirmed the enhanced apoptotic activity in hematopoietic cells and progenitor cells. Antagonists, against P2X receptors and ATP, suramin and oxidized ATP, inhibited the induction of cell death for murine hematopoietic cells. Our data suggest that extracellular nucleotides may provide a novel and powerful tool for regulating the cell fate of hematopoietic stem cells.
Emulsifier‐free emulsion polymerization with ionic comonomer (1976)

Abstract Emulsion polymerization of styrene in the absence of emulsifier with K 2 S 2 O 8 as initiator produced uniform latices. Incorporation of ca. 0.5% ionic comonomer (sodium styrenesulfonate) reduced the particle size from the range 0.5–1.0 μm achieved in prior emulsifier free formulations to a range of 0.15–0.40 μm. Some advantages achieved by incorporation of ionic comonomer were higher polymer content and independently controllable surface charge density. Particle diameter varied as the 0.64 power of the ratio of ionic strength to comonomer, as the −0.20 power of initiator concentration, and as the 0.46 power of monomer content. Kinetic data suggest that copolymerization takes place in the aqueous phase, and that nuclei for particle growth are formed by precipitation of the initially water‐soluble copolymer. The latex is stabilized by sulfonic acid groups of the comonomer, as well as by sulfate end groups from the initiator.
Errata: Three-Dimensional Investigation of the Propagation of Waves in Hollow Circular Cylinders. II [J. Acoust. Soc. Am. <b>31,</b> 573–578 (1959)] (1960)

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Global Structure of Three-Way DNA Junctions with and without Additional Unpaired Bases:  A Fluorescence Resonance Energy Transfer Analysis (1997)

The structure of three-way DNA junctions with and without extrahelical adenine nucleotides in one strand at the branch point of the junction (i.e., An bulges with n = 0, 1, 2, and 3) has been investigated by fluorescence resonance energy transfer. The structure of the junction without bulged nucleotides was found to have a symmetric trigonal geometry. With bulges, the arrangement of the arms becomes asymmetrical. The energy transfer results suggest a model of bulged junctions where the angle between two of the arms is significantly smaller than between the other two pairs of arms. The acute angle becomes smaller as the number of nucleotides in the bulge increases. The FRET efficiencies of the junctions are the same in the presence of Mg++ and Na+ ions.
Antigen-responsive molecular sensor enables real-time tumor-specific imaging (2017)

Antibody-fluorophore conjugates have high potential for the specific fluorescence detection of target cancer cells <i>in vitro</i> and <i>in vivo</i>. However, the antibody-fluorophore conjugates described to date are inappropriate for real-time imaging of target cells because removal of unbound antibody is required to reduce background fluorescence before quantifiable analysis by microscopy. In addition, clinical applications of the conjugates have been limited by persistent background retention due to their long systemic circulation and nonspecific uptake. Here we report fast and real-time near-infrared fluorescence imaging of target cancer cells using an antigen-responsive molecular "on-off" sensor: the fluorescence of trastuzumab-ATTO680 conjugate is dark (i.e., turned off) in the extracellular region, while it becomes highly fluorescent (i.e., turned on) upon binding to the target antigen HER2 on cancer cell surface. This molecular switch enables fast and real-time imaging of target cancer cells <i>in vitro</i> and <i>in vivo</i>.
Microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptase-polymerase chain reaction (2004)

A method is described for enabling safe transit of denatured virus samples for polymerase chain reaction (PCR) identification without the risk of unwanted viable viruses. Cotton swabs dipped in avian infectious bronchitis virus (IBV) or avian pneumovirus (APV) were allowed to dry. Newcastle disease virus and avian influenza viruses were used as controls. Autoclaving and microwave treatment for as little as 20 sec destroyed the infectivity of all four viruses. However, both IBV and APV could be detected by reverse transcriptase (RT)-PCR after autoclaving and as long as 5 min microwave treatment (Newcastle disease virus and avian influenza viruses were not tested). Double microwave treatment of IBV and APV with an interval of 2 to 7 days between was tested. After the second treatment, RT-PCR products were readily detected in all samples. Swabs from the tracheas and cloacas of chicks infected with IBV shown to contain infectious virus were microwaved. Swabs from both sources were positive by RT-PCR. Microwave treatment appears to be a satisfactory method of inactivating virus while preserving nucleic acid for PCR identification.
Altered expression of costimulatory molecules in Behçet’s disease according to clinical activity (2011)

The results of this study suggest that altered expression of PD-L1, CTLA-4 and CD86 may be involved in the pathogenesis of BD.
Age of the Donor Reduces the Ability of Human Adipose-Derived Stem Cells to Alleviate Symptoms in the Experimental Autoimmune Encephalomyelitis Mouse Model (2013)

There is a significant clinical need for effective therapies for primary progressive multiple sclerosis, which presents later in life (i.e., older than 50 years) and has symptoms that increase in severity without remission. With autologous mesenchymal stem cell therapy now in the early phases of clinical trials for all forms of multiple sclerosis (MS), it is necessary to determine whether autologous stem cells from older donors have therapeutic effectiveness. In this study, the therapeutic efficacy of human adipose-derived mesenchymal stem cells (ASCs) from older donors was directly compared with that of cells from younger donors for disease prevention. Mice were induced with chronic experimental autoimmune encephalomyelitis (EAE) using the myelin oligodendrocyte glycoprotein35-55 peptide and treated before disease onset with ASCs derived from younger (<35 years) or older (>60 years) donors. ASCs from older donors failed to ameliorate the neurodegeneration associated with EAE, and mice treated with older donor cells had increased central nervous system inflammation, demyelination, and splenocyte proliferation in vitro compared with the mice receiving cells from younger donors. Therefore, the results of this study demonstrated that donor age significantly affects the ability of human ASCs to provide neuroprotection, immunomodulation, and/or remyelination in EAE mice. The age-related therapeutic differences corroborate recent findings that biologic aging occurs in stem cells, and the differences are supported by evidence in this study that older ASCs, compared with younger donor cells, secrete less hepatocyte growth factor and other bioactive molecules when stimulated in vitro. These results highlight the need for evaluation of autologous ASCs derived from older patients when used as therapy for MS.
Protective Function of Chloroplast 2-Cysteine Peroxiredoxin in Photosynthesis. Evidence from Transgenic Arabidopsis1 (1999)

2-Cysteine peroxiredoxins (2-CPs) constitute a ubiquitous group of peroxidases that reduce cell-toxic alkyl hydroperoxides to their corresponding alcohols. Recently, we cloned 2-CP cDNAs from plants and characterized them as chloroplast proteins. To elucidate the physiological function of the 2-CP in plant metabolism, we generated antisense mutants in Arabidopsis. In the mutant lines a 2-CP deficiency developed during early leaf and plant development and eventually the protein accumulated to wild-type levels. In young mutants with reduced amounts of 2-CP, photosynthesis was impaired and the levels of D1 protein, the light-harvesting protein complex associated with photosystem II, chloroplast ATP synthase, and ribulose-1,5-bisphosphate carboxylase/oxygenase were decreased. Photoinhibition was particularly pronounced after the application of the protein synthesis inhibitor, lincomycin. We concluded that the photosynthetic machinery needs high levels of 2-CP during leaf development to protect it from oxidative damage and that the damage is reduced by the accumulation of 2-CP protein, by the de novo synthesis and replacement of damaged proteins, and by the induction of other antioxidant defenses in 2-CP mutants.
User modelling, dialog structure, and dialog strategy in HAM-ANS (1985)

AI dialog systems are now developing from question-answering systems toward advising systems. This includes:- structuring dialog- understanding and generating a wider range of speech acts than simply information request and answer- user modellingUser modelling in HAM-ANS is closely connected to dialog structure and dialog strategy. In advising the user, the system generates and verbalizes speech acts. The choice of the speech act is guided by the user profile and the dialog strategy of the system.
IL-17 Enhances Tumor Development in Carcinogen-Induced Skin Cancer (2010)

Inflammatory conditions elicited by extrinsic environmental factors promote malignant cell transformation, tumor growth, and metastasis. Although most attention has been focused on innate immune mechanisms of inflammatory carcinogenesis, more recently the role of T cells in cancer promotion has been examined. Although IFN-dependent Th1 responses that promote Stat1 signaling inhibit tumor growth, the role of T helper type 17 responses, and interleukin-17 (IL-17) in particular, has been controversial. Indeed, IL-17 has been reported to either enhance or inhibit the growth of transplantable tumors, depending on the system. Little is known about the role of IL-17 in de novo carcinogenesis. Using IL-17 knockout mice, we examined the role of IL-17 in the classic DMBA/TPA-induced skin carcinogenesis model. Disruption of IL-17 dramatically reduced tumorigenesis in this model in a manner correlated with diminished Stat3 activation in the tumor microenvironment. IL-17 loss reduced Stat3-associated proliferative and antiapoptotic gene expression along with epidermal cell proliferation and hyperplasia. In addition, IL-17 loss was associated with reduced expression of Stat3-regulated chemokines that attract myeloid cells and a decreased infiltration of myeloid cells into the local tumor microenvironment. Together, our findings point to a critical role of the IL-17-Stat3 pathway in supporting cancer-associated inflammation in the tumor microenvironment. Therapeutic approaches that target this pathway may therefore be effective to inhibit carcinogenesis.