gebhart/patrepeval huggingface dataset:
Analisis korelasi dan koefisien lintas sifat-sifat agronomi terhadap komposisi kimia umbi iles-iles (Amorphophallus variabilis) = Correlation and path coefficient analysis of agronomic traits on chemical compositions... (2001)

The aims of this research were to estimate the correlation coefficient among agronomic traits on chemical compositions of Amorphophallus variabilis tither and to evaluate the direct and indirect effects of those traits on chemical compositions of tuber using path analysis.\nThirty six accessions of A. variabilis, obtained/collected from exploration in Yogyakarta, Purworelo, and Magelang, were planted in Randomized Complete Block Design with two blocks as replications. Characters of three plants from each experimental unit were recorded.\nThe result showed that plant height, leaf stalk diameter, and tuber diameter had positive correlations to the tuber weight. The tuber diameter and tuber weight had positive correlations to the ghicomannan content of the tuber. The glucomannan content had positive correlation to the starch content of the tuber and starch content had positive correlation to the calcium oxalate content of the tuber.\nPath cinabwis applied on agronomic traits on tuber weight indicated that the tuber weight was directly affected by leaf stalk diameter and tuber diameter. Path analysis on chemical compositions of tither indicated that ghtcomannan content of the mho- was directly affected by the tuber Ireigln. The tuber weight could he used as the main morphological character for selection of glucomannan content. The largest compound path coefficient of agronomic traits on chemical compositions of tuber via the tuber weight was the tither diameter.\n\nKeywords: A. variabilis, correlation coefficient, path analysis, tuber chemical composition.
Enhancement of Human Melanoma Antigen Expression by IFN-β (2007)

Although many immunotherapeutic investigations have focused on improving the effector limb of the antitumor response, few studies have addressed preventing the loss of tumor-associated Ag (TAA) expression, associated with immune escape by tumors. We found that TAA loss from human melanomas usually results from reversible gene down-regulation, rather than gene deletion or mutation. Previously, we showed that inhibitors of MAPK-signaling pathways up-regulate TAA expression in melanoma cell lines. We have now identified IFN-beta as an additional stimulus to TAA expression, including Melan-A/MART-1, gp100, and MAGE-A1. IFN-beta (but neither IFN-alpha nor IFN-gamma) augmented both protein and mRNA expression of melanocytic TAA in 15 melanoma lines (irrespective of initial Ag-expression levels). Treatment of low Ag melanoma lines with IFN-beta increased expression of melanocyte-lineage Ags, inducing susceptibility to lysis by specific CTLs. Treatment with IFN-beta also enhances expression of class I HLA molecules, thereby inducing both nominal TAA and the presenting HLA molecule. Data from fluorescent cellular reporter systems demonstrated that IFN-beta triggers promoter activation, resulting in augmentation of Ag expression. In addition to enhancing TAA expression in melanomas, IFN-beta also stimulated expression of the melanocytic Ag gp100 in cells of other neural crest-derived tumor lines (gliomas) and certain unrelated tumors. Because IFN-beta is already approved for human clinical use in other contexts, it may prove useful as a cotreatment for augmenting tumor Ag expression during immunotherapy.
Acquired resistance to BRAF inhibition in BRAFV600E mutant gliomas (2016)

Activating mutation of BRAF is a common finding in pediatric gliomas. As many as 14% of high grade and up to 66% of certain subtypes of low grade pediatric glioma have the BRAFV600E mutation. Small molecule inhibitors that selectively target BRAFV600E are FDA approved for melanoma and have shown significant efficacy in treating BRAFV600E glioma in pre-clinical trials. Despite showing initial anti-tumor activity, acquired drug resistance significantly limits the benefit from being treated with BRAFV600E inhibitors. Here, we have identified molecular responses to BRAFV600E inhibitor treatment in human glioma models that have substantial clinical implications. Specifically, we show that BRAFV600E inhibitor resistant cells upregulate pro-survival mediators such as Wnt, and additionally increase receptor tyrosine kinase activity, including EGFR and Axl, promoting resistance to BRAFV600E inhibition. Our results suggest strategies to circumvent acquired resistance to BRAFV600E inhibitor therapy, and thereby improve outcomes for patients with BRAFV600E gliomas.
Evaluation of Phage Display Discovered Peptides as Ligands for Prostate-Specific Membrane Antigen (PSMA) (2013)

The aim of this study was to identify potential ligands of PSMA suitable for further development as novel PSMA-targeted peptides using phage display technology. The human PSMA protein was immobilized as a target followed by incubation with a 15-mer phage display random peptide library. After one round of prescreening and two rounds of screening, high-stringency screening at the third round of panning was performed to identify the highest affinity binders. Phages which had a specific binding activity to PSMA in human prostate cancer cells were isolated and the DNA corresponding to the 15-mers were sequenced to provide three consensus sequences: GDHSPFT, SHFSVGS and EVPRLSLLAVFL as well as other sequences that did not display consensus. Two of the peptide sequences deduced from DNA sequencing of binding phages, SHSFSVGSGDHSPFT and GRFLTGGTGRLLRIS were labeled with 5-carboxyfluorescein and shown to bind and co-internalize with PSMA on human prostate cancer cells by fluorescence microscopy. The high stringency requirements yielded peptides with affinities KD~1 µM or greater which are suitable starting points for affinity maturation. While these values were less than anticipated, the high stringency did yield peptide sequences that apparently bound to different surfaces on PSMA. These peptide sequences could be the basis for further development of peptides for prostate cancer tumor imaging and therapy.
Sustainable development: efficiency and recycling in chemicals manufacturingElectronic supplementary information (ESI) available: appendix A: calculation of carbon and inorganic waste production; appendix B: production of carbon and inorganic wastes from petrochemical processes. See http://www.rsc.org/suppdata/gc/b2/b207546f/ (2002)

Since the Rio Earth Summit in 1992, Sustainable Development has been increasingly accepted as a way to run businesses by balancing economic, environmental and societal responsibilities. Important aspects of environmental responsibility are the utilisation of natural resources and the disposal of wastes. These are addressed here.
The Extraction and Development of Antitumor-Active Polysaccharides from Medicinal Mushrooms in Japan (Review) (1999)

It has been known for many years that most mushrooms with known effects against cancer belong to the family Polyporaceae s.l. (Agaricales s.l.). However, the components responsible for such action have not been clearly defined. In Japan, in 1968, a hot water extract from some edible mushrooms belonging to the family Polyporaceae s.l., showed a marked host-mediated antitumor activity. Since then, numerous researchers have isolated active polysaccharides and have identified them to be (1→3)-β-D-glucopyranans with a (1→6)-β-D-glucosyl branch containing protein. Several antitumor polysaccharides, some hetero-β-glucans and their protein complexes, such as xyloglucans and acidic β-glucan containing uronic acid, are insoluble in water. They were, however, isolated from the extracts using large amounts of dilute alkali with Japanese (8 species) and Chinese (5 species) mushrooms. Several trials have been made to enhance activity by chemical modification, such as polyalcohols formed by a mild Smith degradation and the products formed by BH4-reduction after 104-oxidation. Mushroom polysaccharides are considered to be biological response modifiers (BRM), or immunopotentiators, because of their action mechanism. In Japan, three different polysaccharide antitumor agents have been developed from the fruit body, mycelium, and cultured medium, of three mushroom species.
Discovery of a Magnetic Ionic Liquid [bmim]FeCl4 (2004)

Abstract A magnetic ionic liquid, which shows a strong response to magnetic field, has been discovered. We synthesized an ionic liquid by mixing 1-butyl-3-methylimidazolium chloride ([bmim]Cl) and FeCl3, in anticipation of unique magnetic properties due to the possible local ordering of the magnetic anions. The visible absorption spectroscopy has shown that this liquid contains the high spin FeCl4− as the anion. Raman spectroscopy has indicated that it contains bmim+ as the cation. It has thus been confirmed that the prepared liquid is [bmim]FeCl4. The liquid [bmim]FeCl4 responded strongly to magnetic field. The SQUID measurements have shown that it is paramagnetic having a large magnetic susceptibility of 40.6 × 10−6 emu g−1. The present discovery of a magnetic ionic liquid has opened up a new research area of the magnetism of liquids.
Random Mutagenesis Strategies for Construction of Large and Diverse Clone Libraries of Mutated DNA Fragments (2004)

The first important step toward a successful preparation of large and diverse DNA libraries with desired complexity is to select a suitable mutagenesis strategy. This chapter describes three different methods for random mutagenesis, the use of XL1-red cells, error-prone polymerase chain reaction (PCR), and degenerate oligonucleotides-Pfu (DOP). These mutagenesis strategies possess different benefits and pitfalls; thus, they are differentially useful for production of DNA libraries with different density and complexity. The use of XL1-red, an engineered Escherichia coli with DNA repair deficiency, is one of the simplest mutagenesis and requires no subcloning step. After plasmid encoding DNA of inter-est is transformed into the cells, the mutations are simply generated during each round of DNA replication. The mutation frequency of this method is reported to be 1 base change per 2000 nucleotides; however, it can be slightly increased by extending the culture period to allow the accumulation of more mutations. This strategy is suitable for generation of random mutations with low frequency in a large target DNA. Error-prone PCR is one of the most widely used random mutagenesis. During DNA amplification, misincorporation of nucleotides can be promoted by altering the nucleotide ratio and the concentration of divalent cations in the reaction. We discovered that, by adjusting template concentration, frequency of mutation could be controlled easily and a library with desired mutation rate could be obtained. Additionally, efficiency of subsequent cloning steps to insert the PCR product into plasmid DNA is also a key factor determining size and complexity of the libraries. DOP mutagenesis is a rapid and effective method for random mutagenesis of small DNA and peptides. This strategy uses two chemically synthesized degenerate oligonucleotides as primers. By controlling the positions and ratios of degenerate nucleotides used during oligonucleotide synthesis, it is possible to control both the position and rate of mutation in degenerated region of the primers. The primers are integrated into newly synthesized plasmid DNA by primer extension reaction using Pfu DNA polymerase. After plasmid DNA template encoding wild-type sequence is eliminated from the reaction by DpnI digestion, the pool of mutagenized plasmids can then be used directly in screening procedures.
Preconcentration of ultratrace rare earth elements in seawater with 8-hydroxyquinoline immobilized polyacrylonitrile hollow fiber membrane for determination by inductively coupled plasma mass spectrometry (1999)

8-Hydroxyquinoline immobilized on polyacrylonitrile hollow fiber membrane was synthesized and used for the preconcentration of rare earth elements (REEs) and the separation of the matrix in seawater prior to their determination by inductively coupled plasma mass spectrometry (ICP-MS). Both the capacity and chemical stability of the modified polyacrylonitrile hollow fiber membrane have been evaluated. The optimum experimental conditions, such as pH, sample flow rate, volume of eluents, were investigated. The concentration of REEs in seawater and the separation of the matrix components can be achieved with a concentration factor of at least 300 times. The recommended method has been applied for the concentration and determination of REEs in coastal seawater. Results indicated that the recovery of REEs ranged from 91% to 107%, and the relative standard deviations were found to be less than 5%. A comparison was made between the REE levels determined by this method and the literature reports, and a reasonable agreement was obtained.
Device and circuit approaches for improved wireless communications transmitters (1999)

Next-generation wireless communication will require improved transmitter technology for a variety of applications, from advanced handsets to improved base stations. The UCSD Center for Wireless Communications has embarked on an ambitious program to develop improved power amplifier devices, architectures, and circuits for next-generation wireless communications applications. This article summarizes some emerging power amplifier technologies for future communication systems, including new linearization approaches, power management techniques, and new semiconductor devices.
The Next Frontier (2004)

The history of the addictions field has been one of evolving paradigms (organizing constructs), evolving core technologies and evolving definitions of the field’s niche in the larger culture whose needs it must serve. This article traces the evolution of the field’s organizing paradigms through three overlapping stages, a problem-focused stage, an intervention-focused stage and an emerging solution-focused stage. These paradigms can be viewed as competing models but are best viewed as developmental stages, with each preparing the emergence of the next.
ARFI imaging of thermal lesions in ex vivo and in vivo soft tissues (2004)

The ability of ARFI imaging to monitor the ablation of soft tissues both ex vivo and in vivo was investigated. Thermal lesions were induced both in freshly excised bovine liver samples and in myocardial tissue of live sheep. While conventional sonography was unable to visualize induced lesions, ARFI imaging was capable of monitoring lesion size and boundaries. Good agreement was observed between lesion size in ARFI images and in results from pathology. The fact that ARFI imaging requires no additional equipment aside from that needed for conventional ultrasonic imaging makes it a promising modality for monitoring ablation procedures in vivo, especially in situations where sonography is already involved as a guiding mechanism, such as in many procedures requiring precise catheter placement.
Defects of Zeolite Membranes: Characterization, Modification and Post-treatment Techniques (2015)

Characterization of zeolite membrane defects including SEM, dye permeation, bubble point and permporometry methods are presented. Experimental setup, governing equations and step by step procedure for obtaining pore sizes are discussed for the most useful method, i.e., the permporometry. A complete review on the techniques for elimination of defects including chemical vapor and liquid depositions, coke deposition, surface coatings, and hydrothermal treatments is presented. For each method, experimental setup, various modification conditions as well as performance enhancement of the membranes are comprehensively discussed. Finally, comparison between different methods is performed to find the more efficient techniques.
Pretreatment serum level of HER2/nue as a prognostic factor in metastatic prostate cancer patients about to undergo endocrine therapy (2006)

The pretreatment serum HER2 level may be a useful independent prognostic factor that is associated with a high risk of biochemical recurrence in metastatic prostate cancer patients about to undergo endocrine therapy.
Calcium‐Alginate Nanoparticles Formed by Reverse Microemulsion as Gene Carriers (2005)

Abstract Natural biopolymers are widely used in the field of drug and gene delivery. In this study, alginate nanoparticles were prepared using water‐in‐oil microemulsion as a template followed by calcium crosslinking of guluronic acid units of alginate polymer. After collected by ultracentrifugation, alginate nanoparticles were analyzed by electron microscopy to obtain the size and morphology which were varied with the ratio of water, oil, and surfactant used. To examine the potency of Ca‐alginate nanoparticles as carriers for gene delivery, GFP‐encoding plasmids were encapsulated in these nanoparticles to investigate the degree of endocytosis by NIH 3T3 cells and ensuing transfection rate. Our results showed that Ca‐alginate nanoparticles with an average size around 80 nm in diameter are very efficient gene carriers, in comparison with plasmid DNA condensed by polyethyleneimine (PEI).
Interactions of thymidine, hyperthermia, and cis-diammine-1,1-cyclobutane dicarboxylate platinum (II) in human T-cell leukemia. (1989)

Using JM and MOLT3, two human T-cell acute lymphoblastic leukemia cell lines, we investigated the ability of 24-h thymidine exposures to enhance the cytotoxicity of cis-diammine-1,1-cyclobutane dicarboxylate platinum (II) (carboplatin). Clinically achievable thymidine concentrations (for 24 h) significantly enhanced carboplatin killing. Unexpectedly, thymidine-carboplatin enhancement was as great at a relatively low 200-micrograms thymidine/ml as at the clinically much more toxic range of 1000 micrograms/ml. For a constant thymidine concentration (500 micrograms/ml), thymidine-carboplatin interaction increased with longer thymidine exposures until about 16 to 24 h. Thymidine and 41.8 degrees C hyperthermia (for 1 h) together enhanced carboplatin killing significantly more than did hyperthermia-carboplatin or thymidine-carboplatin combinations. These results show that relatively brief, presumptively nonmyelosuppressive thymidine exposures can significantly increase carboplatin killing. Carboplatin-thymidine killing can then be further augmented by 41.8 degrees C hyperthermia.
A robust timing synchronization design in OFDM systems-part I: low-mobility cases (2007)

In this paper we are interested in designing a robust timing synchronization algorithm for OFDM systems that utilize pilot-aided channel estimation. We first characterize the impact of timing errors on the performance of a pilot-aided OFDM system. We derive analytical expressions for average channel estimation error variance in the presence of timing errors in high delay spread fading environments. The derived expressions show that pilot-aided channel estimators are considerably sensitive to timing synchronization errors due to the impact of rotations in different bases. We then show how to utilize this sensitivity to design a robust timing synchronization algorithm, without training overhead. The proposed algorithm is a cross-block design that uses channel estimation information to improve timing synchronization. We confirm our analytical results by simulating the proposed algorithm in high delay spread fading environments. In this paper, i.e. part I, we focus on timing synchronization for low-mobility cases. The analysis and results are then extended to high-mobility applications in part II.
Levels of CD40 expression on dendritic cells dictate tumour growth or regression (2007)

Tumour regression requires activation of T cells. It has been shown that the interaction between T cell-expressed CD40-ligand (CD40-L) and antigen-presenting cell-expressed CD40 plays a crucial role in T cell activation. CD40-L- or CD40-deficient mice are susceptible to tumour growth. CD40-based therapies are also shown to control tumour growth significantly, suggesting that CD40-CD40-L interaction induces anti-tumour T cell responses and tumour regression. We demonstrate that the anti-tumour T cell response can be modulated reciprocally as a function of the levels of CD40 expression. At low expression levels, CD40 promotes tumour growth; at higher expression levels, CD40 induces tumour-regressing T cell response. Dendritic cells (DC) sorted onto major histocompatibility complex (MHC)-II expression are found to be similar in CD40 and CD80 expression. The MHC-II(hi)/CD40(hi) DC induce interleukin (IL)-12-dominated and T helper 1 (Th1)-type response, whereas MHC-II(lo)/CD40(lo) DC promote high IL-10 and Th2-type T cells. The T cells induced by these DC also differ in terms of regulatory T cell markers, lymphocyte activation gene-3 (LAG-3) and glucocorticoid-induced tumour necrosis factor (TNF) receptor family-related gene (GITR). Thus, we report for the first time that CD40-induced effector T cell response depends on CD40 expression levels in vivo.
Resource Allocation and Link Adaptation in LTE and LTE Advanced: A Tutorial (2014)

Resource allocation and link adaptation in Long-Term Evolution (LTE) and LTE Advanced are discussed with focus on the location and formatting of the pertinent reference and control signals, as well as the decisions they enable. In particular, after reviewing the units for resource allocation and the time-frequency resource grid, the enabled resource-allocation modes and their purposes are reviewed. A detailed description of the way the resource allocations are encoded under these different modes is also given. Similarly, the various methods of link adaptation, including power control and rate control, both through the use of adaptive modulation and coding and hybrid automatic repeat request, are reviewed. The control signaling encoding for link adaptation is provided in detail, as is the encoding of channel state feedback for the purposes of link adaptation and resource allocation.
Predicting Carbon-13 Nuclear Magnetic Resonance Chemical Shielding Tensors in Zwitterionic L-Threonine and L-Tyrosine via Quantum Chemistry (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPredicting Carbon-13 Nuclear Magnetic Resonance Chemical Shielding Tensors in Zwitterionic L-Threonine and L-Tyrosine via Quantum ChemistryAngel C. de Dios, David D. Laws, and Eric OldfieldCite this: J. Am. Chem. Soc. 1994, 116, 17, 7784–7786Publication Date (Print):August 1, 1994Publication History Published online1 May 2002Published inissue 1 August 1994https://pubs.acs.org/doi/10.1021/ja00096a039https://doi.org/10.1021/ja00096a039research-articleACS PublicationsRequest reuse permissionsArticle Views164Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
DETECTION OF THE PREICTAL TRANSITION STATE IN SCALP-SPHENOIDAL EEG RECORDINGS (1996)

Iasemidis, Leon D.*+; Pappas, Konstantinos E.+; Gilmore, Robin L.*⁁; Roper, Stephen N.#; Sackellares, J Chris*⁁ Author Information