gebhart/patrepeval huggingface dataset:
Large‐scale feasibility of gene transduction into human CD34<sup>+</sup> cell‐derived dendritic cells by adenoviral/polycation complex (2000)

With a view to using multiple injections of anti‐cancer dendritic cell (DC)‐based vaccines, we evaluated the feasibility of the adenoviral transduction of large amounts of human CD34 + cell‐derived DCs, and analysed the persistence of the transgene expression and the integrity of DC functional activity after the transduction/cryopreservation procedures. Mature DCs generated from highly enriched human CD34 + cells were transduced by a recombinant adenovirus (rAd‐MFG) that carried a modified, membrane‐exposed, alkaline phosphatase (AP) sequence as the reporter gene. Cationic lipids such as LipofectAmine or poly‐ l ‐lysine were mixed with the viral particles before the transduction of the target cells. The highest transduction efficiency was obtained at a multiplicity of infection (MOI) rate of 500 (AP + DCs: 50 ± 2%, viability = 95%) under both small‐ and large‐scale conditions. The addition of poly‐ l ‐lysine or LipofectAmine increased the percentage of transduced cells at an MOI of 500 (CD1a + /AP + cells = 85 ± 3% and 80 ± 2% respectively). Polycations made it possible to reduce the amounts of viral particles, with high efficiency of transduction being achieved at a MOI of 100 with 10 μg/ml poly‐ l ‐lysine (CD1a + /AP + : 68 ± 9%) or 30 μg/ml LipofectAmine (CD1a + /AP + : 60 ± 7%). Evaluation of the immunophenotype of the transduced DCs showed that the lack of a DC subpopulation was more susceptible to adenoviral transduction. Cryopreservation of transduced DCs did not modify the viability or percentage of AP + cells that maintain antigen‐presenting cell (APC) functions. These findings indicate the efficacy of this method for the transduction of large amounts of CD34 + cell‐derived DCs using small quantities of adenoviral vector mixed with polycations. Cryopreservation of transduced DCs did not damage their viability or APC functions, thus making it possible to plan multiple injections of engineered DC‐based vaccines.
Rapid Redistribution of Synaptic PSD-95 in the Neocortex In Vivo (2006)

Most excitatory synapses terminate on dendritic spines. Spines vary in size, and their volumes are proportional to the area of the postsynaptic density (PSD) and synaptic strength. PSD-95 is an abundant multi-domain postsynaptic scaffolding protein that clusters glutamate receptors and organizes the associated signaling complexes. PSD-95 is thought to determine the size and strength of synapses. Although spines and their synapses can persist for months in vivo, PSD-95 and other PSD proteins have shorter half-lives in vitro, on the order of hours. To probe the mechanisms underlying synapse stability, we measured the dynamics of synaptic PSD-95 clusters in vivo. Using two-photon microscopy, we imaged PSD-95 tagged with GFP in layer 2/3 dendrites in the developing (postnatal day 10-21) barrel cortex. A subset of PSD-95 clusters was stable for days. Using two-photon photoactivation of PSD-95 tagged with photoactivatable GFP (paGFP), we measured the time over which PSD-95 molecules were retained in individual spines. Synaptic PSD-95 turned over rapidly (median retention times tau(r) is approximately 22-63 min from P10-P21) and exchanged with PSD-95 in neighboring spines by diffusion. PSDs therefore share a dynamic pool of PSD-95. Large PSDs in large spines captured more diffusing PSD-95 and also retained PSD-95 longer than small PSDs. Changes in the sizes of individual PSDs over days were associated with concomitant changes in PSD-95 retention times. Furthermore, retention times increased with developmental age (tau(r) is approximately 100 min at postnatal day 70) and decreased dramatically following sensory deprivation. Our data suggest that individual PSDs compete for PSD-95 and that the kinetic interactions between PSD molecules and PSDs are tuned to regulate PSD size.
Computed tomography of gastrointestinal inflammation after bone marrow transplantation (1986)

Overwhelming secretory diarrhea can be a major complication after bone marrow transplantation, associated usually with acute graft-versus-host disease (AGVHD). Radiographic evaluation may be hampered by nausea, vomiting, or debilitation. Computed tomography (CT) in seven such patients demonstrated diffuse wall thickening in the small intestine, colon, and/or mesentery. In two cases, prolonged adherence of oral contrast material to the luminal surface resulted in bizarre patterns of coating. In two others, a layer of low attenuation within the thickened wall produced a target appearance consistent with submucosal edema or hemorrhage. Small bowel dilatation and fold enlargement was the only finding in one patient. The role that superinfection of the gastrointestinal tract with opportunistic organisms can play in this immunocompromised group of patients is less well established. In this group of patients, the findings were due to AGVHD in two, AGVHD and viral enteritis in two, and viral enteritis alone in three. CT may be an alternative to routine contrast studies in assessing the extent of gastrointestinal tract involvement after bone marrow transplantation. Neither contrast studies nor CT were able to differentiate between AGVHD and viral enteritis.
A Novel 28 GHz Beam Steering Array for 5G Mobile Device With Metallic Casing Application (2017)

The design of a novel practical 28 GHz beam steering phased array antenna for future fifth generation mobile device applications is presented in this communication. The proposed array antenna has 16 cavity-backed slot antenna elements that are implemented via the metallic back casing of the mobile device, in which two eight-element phased arrays are built on the left- and right-side edges of the mobile device. Each eight-element phased array can yield beam steering at broadside and gain of >15 dBi can be achieved at boresight. The measured 10 dB return loss bandwidth of the proposed cavity-backed slot antenna element was approximately 27.5-30 GHz. In addition, the impacts of user's hand effects are also investigated.
The challenge of broadcasting at intersections in Vehicular Adhoc Networks (2010)

Vehicular Ad hoc Networks (VANETs) are new network environment for intelligent transportation systems. One of the fundamental services in VANET is broadcasting, that is a challenging task due to different road topologies and network conditions. One of its challenges is broadcasting at intersections, that because vehicles in different roads constituting an intersections can affect on broadcast process of other roads, message may not be propagated in some directions. In this paper we propose a method that by classifying vehicles based on their location and the road they move in solves this problem. Our method is simulated in NS2, and simulation results show our method assures broadcasting in all directions.
Establishing a sensitive fluorescence-based quantification method for cyclic nucleotides (2020)

We found that determination cAMP in lysates obtained from cell assays or tissue samples by purified Epac1-camps is a robust, fast, and sensitive assay suitable for routine and high throughput analyses.
Peculiarities of the Continuous Glucose Monitoring Data Stream and Their Impact on Developing Closed-Loop Control Technology (2008)

Therapeutic advances in type 1 diabetes (T1DM) are currently focused on developing a closed-loop control system using a continuous glucose monitor (CGM), subcutaneous insulin delivery, and a control algorithm. Because a CGM assesses blood glucose indirectly (and therefore often inaccurately), it limits the effectiveness of the controller. In order to improve the quality of CGM data, a series of analyses are suggested. These analyses evaluate and compensate for CGM errors, assess risks associated with glucose variability, predict glucose fluctuation, and forecast hypo- and hyperglycemia. These analyses are illustrated with data collected using the MiniMed CGMS® (Medtronic, Northridge, CA) and Freestyle Navigator(™) (Abbott Diabetes Care, Alameda, CA). It is important to remember that traditional statistics do not work with CGM data because consecutive CGM readings are highly interdependent.
Expression Studies of the Zeaxanthin Epoxidase Gene in<i>Nicotiana plumbaginifolia</i>  (1998)

Abscisic acid (ABA) is a plant hormone involved in the control of a wide range of physiological processes, including adaptation to environmental stress and seed development. In higher plants ABA is a breakdown product of xanthophyll carotenoids (C40) via the C15 intermediate xanthoxin. The ABA2 gene of Nicotiana plumbaginifolia encodes zeaxanthin epoxidase, which catalyzes the conversion of zeaxanthin to violaxanthin. In this study we analyzed steady-state levels of ABA2 mRNA in N. plumbaginifolia. The ABA2 mRNA accumulated in all plant organs, but transcript levels were found to be higher in aerial parts (stems and leaves) than in roots and seeds. In leaves ABA2 mRNA accumulation displayed a day/night cycle; however, the ABA2 protein level remained constant. In roots no diurnal fluctuation in mRNA levels was observed. In seeds the ABA2 mRNA level peaked around the middle of development, when ABA content has been shown to increase in many species. In conditions of drought stress, ABA levels increased in both leaves and roots. A concomitant accumulation of ABA2 mRNA was observed in roots but not in leaves. These results are discussed in relation to the role of zeaxanthin epoxidase both in the xanthophyll cycle and in the synthesis of ABA precursors.
Analysis and experimental validation of performance for an axial flux permanent magnet brushless DC motor with powder iron metallurgy cores (1997)

A small axial flux permanent magnet (AFPM) brushless DC servomotor with powder iron metallurgy cores has been designed and built and its results are presented in this paper. To predict the motor performance, 2-D and 3-D finite element analyses have been performed respectively, and the results are compared in terms of computations of the no-load field and the cogging torque. Finally, the computed results and the predicted characteristics are validated by measurements on a prototype AFPM motor.
Theory in vivo. (1993)

Due to a formerly held assumption that psychoanalytic theories operate exclusively in the positivistic manner associated with the natural sciences, current thinking on how they operate specifically or in their own terms is addressing a long-neglected task. In this paper it is suggested that inherent and acquired modes of theorizing interplay when an analyst is working; and that a dialogue involving these two modes which began in Freud's inner life has evolved into our communal conceptual discourse. A series of situationally connected interchanges with a female analysand, over a two-week period, is presented. An attempt is made to demonstrate some theorizing, not 'in vitro'--'in the test-tube' of abstraction, but where most use and discovery of theory happens, 'in vivo'--within the living experience of therapeutic analysis.
Analyzing search engine advertising (2008)

The phenomenon of sponsored search advertising is gaining ground as the largest source of revenues for search engines. Firms across different industries have are beginning to adopt this as the primary form of online advertising. This process works on an auction mechanism in which advertisers bid for different keywords, and final rank for a given keyword is allocated by the search engine. But how different are firm's actual bids from their optimal bids? Moreover, what are other ways in which firms can potentially benefit from sponsored search advertising? Based on the model and estimates from prior work [10], we conduct a number of policy simulations in order to investigate to what extent an advertiser can benefit from bidding optimally for its keywords. Further, we build a Hierarchical Bayesian modeling framework to explore the potential for cross-selling or spillovers effects from a given keyword advertisement across multiple product categories, and estimate the model using Markov Chain Monte Carlo (MCMC) methods. Our analysis suggests that advertisers are not bidding optimally with respect to maximizing profits. We conduct a detailed analysis with product level variables to explore the extent of cross-selling opportunities across different categories from a given keyword advertisement. We find that there exists significant potential for cross-selling through search keyword advertisements in that consumers often end up buying products from other categories in addition to the product they were searching for. Latency (the time it takes for consumer to place a purchase order after clicking on the advertisement) and the presence of a brand name in the keyword are associated with consumer spending on product categories that are different from the one they were originally searching for on the Internet.
On-Line Latex Particle Size Determination by Dynamic Light Scattering (1991)

The use of on-line dynamic light scattering (DLS) in monitoring latex particle growth in a continuous pilot scale reactor is reported. The weight average diameter of the latex is measured simultaneously using on-line turbidimetry and the results from the two methods are compared. A new design for the on-line DLS apparatus involving the use of a remote sensor and fiber optics, for a more robust performance in industrial environments, is described.
Photoresist-enhanced wafer charging during high current ion implantation (2002)

Various experiments are described involving the influence of patterned photoresist (PR) on wafer charging during high current arsenic implantation. Use of Stanford 100-mm CHARM-2 test chips allows measurement of both potentials and currents delivered to a wafer during implantation-thus allowing extraction of the J-V characteristic of the beam itself. The results indicate that the placement of the PR is of crucial importance: photoresist on field oxide has a moderate influence on charging, whereas PR on a charge-collection-electrode increases positive charging dramatically. Other PR charging effects discussed include: outgassing, resist thickness, wafer blocked-area (area unable to conduct current to the substrate), wafer surface secondary electron coefficient, and PR surface currents. Also, a model of the ion beam-treated as a plasma-is given to aid in the understanding of PR-enhanced charging.
SCINTILLATION CAMERA WITH MULTICHANNEL COLLIMATORS. (1964)

The scintillation camera is a sensitive electronic instrument for taking pie tures of the distribution of gamma-ray and positron-emitting isotopes in vivo. The pictures are similar to those obtained from mechanical scanners, but they are produced in much less time. No scanning is employed because the scintillation camera is sensitive to all parts of its field of view during the entire exposure time. To obtain an image of activity distribution a collimator first projects a gamma-ray image of the subject onto a scintillator. The instrument described here uses a single sodium iodide crystal 11% inches in diameter by 3@inch thick. Coupled to the crystal through an optical light guide is a close-packed hexagonal array of 19 multiplier phototubes. The phototubes view overlapping areas in the seintillator so that light from each scintillation divides among the 19 tubes. The combination of scintillator, light guide, and phototubes is called an image detector (1). The phototubes are connected to an analog computer that identi fies the X and Y coordinates and the brightness of each scintillation occurring in the crystal. All photopeak scintillations are reproduced on an oscilloscope as point flashes of light in the same relative positions in which they occurred in the scintillator. The flashes are photographed over a period of time, and an image of the subject results. To obtain the best combination of sensitivity and resolution for a given subject and radionuclide, the optimum collimation method should be used. A brief account of the three collimating methodsâ€”pinhole, multichannel, and positron coincidenceâ€”has been given (2). For positron emitters, coincidence collimation gives excellent sensitivity and resolution for both large and small subjects. For small subjects containing gamma-ray emitters, pinhole collimation is the method of choice. It is used to obtain high-resolution pictures of small subjects such as the thyroid gland. However, for large gamma-ray emitting subjects, such as the brain or liver, collimators with large numbers of parallel holes (2-9) give the best com bination of sensitivity and resolution. A drawing of this type of collimator is shown with the scintillation camera image detector in Figure 1.
Correlation between asparaginase sensitivity and asparagine synthetase protein content, but not mRNA, in acute lymphoblastic leukemia cell lines (2007)

These observations provide an explanation for the ASNase sensitivity of ALL cells and demonstrate the importance of measuring ASNS protein rather than mRNA in predicting ASNase responsiveness.
Hardware architecture for on-chip unsupervised online neural spike sorting (2013)

Microelectrode arrays can acquire neural signals in parallel from multiple channels. Spike sorting has emerged as one of the most significant challenges in multichannel systems. An ideal spike sorting system must be implantable, unsupervised, online and scalable to hundreds of channels. This paper proposes a novel hardware architecture for on-chip and unsupervised neural spike sorting with Teager Energy Operator detection, Zero-Crossing Features and an online clustering algorithm, MCK Classifier, which is a modification of the standard K-Means. The reported classifier gives an average detection-classification accuracy of 82% at typical SNR of 7dB, which is within 2% of the standard K-Means classifier.
Good Metabolic Control Is Associated With Better Quality of Life in 2,101 Adolescents With Type 1 Diabetes (2001)

In a multiple regression model, lower HbA(1c) was significantly associated with better adolescent-rated QOL on all four subscales and with lower perceived family burden as assessed by parents and health professionals.
A Unified Framework for Detection of Suspicious and Anomalous Beahvior from Spatio-Temporal Traces. (2014)

This paper presents a summary of the doctoral dissertation of the author on the topic of learning patterns of agent behavior from sensor data. Povzetek: Članek predstavlja povzetek doktorske disertacije avtorja, ki obravnava temo učenja vzorcev obnašanja agenta iz senzorskih podatkov.
On the application of thermoluminescence for probing the energetic disorder in amorphous molecular solids (1997)

The low-temperature thermoluminescence (TL) technique has been applied for probing the energetic disorder in doped poly(N-epoxypropylcarbazole)(PEPC) polymer films. Strong polar dopants were used for varying the degree of dipolar disorder. It was found that (1) the TL of the studied systems can be well interpreted in terms of the disorder model and (2) TL spectroscopy can provide the shape of the deepest part of the localised state distribution and the degree of energetic disorder as well. The charge trap distribution on the high-temperature side of the TL peak appears to be a Gaussian function and its halfwidth correlates well with the disorder parameter obtained from charge transport measurements. An unusual electric field effect on the TL of PEPC doped with strong polar dopants was found and is explained in terms of the contribution of the charge-dipole interaction to the energy of localised states. This effect indicates that the TL phenomenon for the studied systems cannot be explained purely by molecular γ-relaxation in the polymer. © 1997 John Wiley & Sons, Ltd.
Effects of hemorrhagic serum on interleukin-2 generation and utilization (1988)

Exposure of normal peripheral blood mononuclear cells (PBMCs) to serum obtained from hemorrhaged animals produces marked depression of mitogen-induced cellular proliferation. In order to examine the mechanism which underlies its suppressive action, the effects of hemorrhagic serum on the production and utilization of interleukin-2 (IL-2) by PBMCs in mitogen-stimulated cultures were investigated. The production of IL-2 by PBMCs obtained from normal animals was reduced by approximately 90% after these cells were washed with hemorrhagic serum for 60 sec at 0 degrees C. Cells cultured in hemorrhagic serum showed greater than 30% reduction in proliferative response to phytohemagglutinin, and no significant change in the degree of suppression was produced by adding recombinant IL-2 to the cell cultures. PBMCs cultured with recombinant IL-2 and hemorrhagic serum, but not mitogen, proliferated significantly less than did cells cultured with IL-2 and normal serum. These findings suggest that although hemorrhagic serum produces a marked decrease in IL-2 generation, the suppressive effects of hemorrhagic serum on mitogen-induced lymphocyte proliferation are not due to this decrease in IL-2 production.
Hypoxia modulates the undifferentiated phenotype of human renal inner medullary CD133<sup>+</sup>progenitors through Oct4/miR-145 balance (2011)

Low-oxygen tension is an important component of the stem cell microenvironment. In rodents, renal resident stem cells have been described in the papilla, a relatively hypoxic region of the kidney. In the present study, we found that CD133(+) cells, previously described as renal progenitors in the human cortex, were enriched in the renal inner medulla and localized within the Henle's loop and thin limb segments. Once isolated, the CD133(+) cell population expressed renal embryonic and stem-related transcription factors and was able to differentiate into mature renal epithelial cells. When injected subcutaneously in immunodeficient mice within Matrigel, CD133(+) cells generated canalized structures positive for renal specific markers of different nephron segments. Oct4A levels and differentiation potential of papillary CD133(+) cells were higher than those of CD133(+) cells from cortical tubuli. Hypoxia was able to promote the undifferentiated phenotype of CD133(+) progenitors from papilla. Hypoxia stimulated clonogenicity, proliferation, vascular endothelial growth factor synthesis, and expression of CD133 that were in turn reduced by epithelial differentiation with parallel HIF-1α downregulation. In addition, hypoxia downregulated microRNA-145 and promoted the synthesis of Oct4A. Epithelial differentiation increased microRNA-145 and reduced Oct4 level, suggesting a balance between Oct4 and microRNA-145. MicroRNA-145 overexpression in CD133(+) cells induced downrelation of Oct4A at the protein level, inhibited cell proliferation, and stimulated terminal differentiation. This study underlines the role of the hypoxic microenvironment in controlling the proliferation and maintaining a progenitor phenotype and stem/progenitor properties of CD133(+) cells of the nephron. This mechanism may be at the basis of the maintenance of a CD133(+) population in the papillary region and may be involved in renal regeneration after injury.