gebhart/patrepeval huggingface dataset:
Total Synthesis of (+)-Ambruticin (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTTotal Synthesis of (+)-AmbruticinPing Liu and Eric N. JacobsenView Author Information Department of Chemistry and Chemical Biology Harvard University, Cambridge, Massachusetts 02138 Cite this: J. Am. Chem. Soc. 2001, 123, 43, 10772–10773Publication Date (Web):October 4, 2001Publication History Received20 August 2001Published online4 October 2001Published inissue 31 October 2001https://pubs.acs.org/doi/10.1021/ja016893shttps://doi.org/10.1021/ja016893srapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views10114Altmetric-Citations202LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Alcohols,Aldehydes,Hydroformylation,Organic synthesis,Stereoselectivity Get e-Alerts
Characterization of genomic alterations associated with glioma progression by comparative genomic hybridization. (1996)

Genomic alterations associated with glioma progression were determined by comparative genomic hybridization (CGH) 30 tumors from 15 patients with primary gliomas of World Health Organization (WHO) grade II that on recurrence showed progression to malignant gliomas of WHO grades III or IV (five cases of astrocytoma grade II (A II) to grade III (AA III), five cases of A II to glioblastoma multiforme grade IV (GBM) and five cases of oligodendroglioma grade II (O II) to grade III (AO III)). All tumors were additionally screened for p53 mutations by single strand conformational polymorphism and heteroduplex analysis of exons 5-8, followed by direct sequencing. Mutations of p53 were found in the primary and recurrent tumors of all cases of A II progressing to GBM and three of five cases of A II recurring as AA III. Alterations identified by CGH in more than one primary A II included losses on Xp (3/10) and 5p (2/10), gains on 8q and 19p (2/10 each), and gain/amplification on 12p (2/10). Common progression associated changes found in AA III or GBM were losses on 4q, 9p, 10q, 11p, 13q (4/10 each) and gains on 1q, 6p, 20q (2/10 each). The most frequent amplification site was located on 12p13 (1/10 A II, 3/5 AA III, 1/5 GBM). Other amplified chromosomal regions were 13q32-q34 (1/10 AII, 2/5 GBM), 7q31-qter (1/5 AA III, 1/5 GBM), 12q22-qter and 18p (1/5 AA III). In contrast to the astrocytic gliomas, only one of five oligodendroglial cases showed a p53 mutation. Genetic abnormalities identified by CGH to occur more than once were restricted to four chromosomes (1, 4, 9 and 19). Our results provide a comprehensive overview of the genomic alterations associated with the progression of individual gliomas and substantiate the hypothesis that glioma progression is associated with a cumulative acquisition of multiple genetic changes.
Irradiated Donor Leukocytes Promote Engraftment of Allogeneic Bone Marrow in Major Histocompatibility Complex Mismatched Recipients Without Causing Graft-Versus-Host Disease (1999)

Graft rejection in allogeneic bone marrow transplantation (BMT) can occur when donor and recipient are mismatched at one or more major histocompatibility complex (MHC) loci. Donor T cells can prevent graft rejection, but may cause fatal graft-versus-host disease (GVHD). We tested whether irradiation of allogeneic donor lymphocytes would preserve their graft-facilitating activity while inhibiting their potential for GVHD. Infusions of irradiated allogeneic T cells did not cause GVHD in MHC-mismatched SJL → (SJL × C57BL6) F1, C57BL6 → B10.RIII, and C57BL6 → B10.BR mouse donor → recipient BMT pairs. The 60-day survival among MHC-mismatched transplant recipients increased from 2% (BM alone) to up to 75% among recipients of BM plus irradiated allogeneic splenocytes. Optimal results were obtained using 50 × 106 to 75 × 106 irradiated donor splenocytes administered in multiple injections from day −1 to day +1. Recipients of an equal number of nonirradiated MHC-mismatched donor splenocytes uniformly died of acute GVHD. The graft facilitating activity of the irradiated allogeneic splenocytes was mediated by donor T cells. Irradiation to 7.5 Gy increased nuclear NFκB in T cells and their allospecific cytotoxicity. Irradiated T cells survived up to 3 days in the BM of MHC-mismatched recipients without proliferation. Recipients of irradiated allogeneic splenocytes and allogeneic BM had stable donor-derived hematopoiesis without a significant representation of donor splenocytes in the T-cell compartment. Irradiated allogeneic T cells thus represent a form of cellular immunotherapy with time-limited biologic activity in vivo that can facilitate allogeneic BMT without causing GVHD.
Spinal cord stimulation versus other therapies in patients with Refractory Angina: A meta-analysis. (2017)

SCS appears to be a safe and effective management for RA although it has similar efficacy when compared to PMR or CABG as a potential replacement therapy. However, before this method is used as a conventional treatment, more high-quality designed multicenter RCTs are needed.
[Effects of paroxetine with or without zolpidem on depression with insomnia: a multi-center randomized comparative study]. (2007)

selective serotonin reuptake inhibitor antidepressant combined with hypnotic augments the effects of antidepressant on the depressive and anxiety symptoms.
Photocleavable Protecting Groups as Nucleobase Protections Allowed the Solid-Phase Synthesis of Base-Sensitive SATE-Prooligonucleotides (1999)

The first synthesis of oligodeoxynucleotide heteropolymers carrying base-sensitive S-pivaloylthioethyl (t-Bu-SATE) phosphotriester linkages has been performed. It is based on the use of 6-nitroveratryloxycarbonyl (NVOC) and 2,2‘-bis(2-nitrophenyl)ethoxycarbonyl (diNPEOC) groups as nucleobase protections in combination with photolysis deprotection. The synthesis was realized using the phosphoramidite approach on solid support bearing a 1-(o-nitrophenyl)-1,3-propanediol linker. The removal of the protecting groups and the cleavage of the oligonucleotides from the solid support were accomplished in a single photolysis procedure upon UV irradiation at wavelengths >300 nm. Faster deprotection rates were observed for diNPEOC-protected nucleosides and oligomers than with NVOC-protected ones. The synthesis of pentanucleoside t-Bu-SATE-phosphotriesters d(5‘TpCpCpCpTp3‘), d(5‘TpApApApAp3‘), and d(5‘TpGpGpGpTp3‘) and of dodecanucleoside t-Bu-SATE-phosphotriesters and -phosphorothioate d(5‘ApCpApCpCpCpApApTpTpCpTp3‘) and d(5‘ApGpApApTpTpGpGpGpTpGpTp3‘) demonstrated the efficiency of the method.
Sudden cardiac death: the search for a non-invasive means to detect the electrical substrate for the development of life-threatening cardiac arrhythmias. (1985)

Cardiovascular diseases remain the major cause of death in the adult North American population. Most of these deaths are sudden, occurring secondary to ventricular tachycardia/fibrillation (VT/VF). Based on the results of recent clinical trials, it seems likely that many of these deaths could be prevented if reliable means were available to select those patients at highest risk. To date, however, no totally satisfactory means to establish risk has been identified. The purpose of this paper is to review the currently utilized techniques and to draw attention to some new and potentially useful technology involving computer processing of surface recorded electrocardiographic (ECG) signals. ECG monitoring during rest and activity is neither sensitive nor specific. Invasive studies, using programmed ventricular stimulation to reproduce clinical arrhythmias, have proven extremely useful in the management of patients with recurrent VT/VF; this technique allows the selection of effective therapy as documented by subsequent inability to induce the arrhythmia. However, this technique does not have broad applicability given the complexity of these studies and the significant potential for morbidity. Recent efforts have focused on the detection of the abnormal electrical substrate giving rise to VT/VF by using body-surface electrocardiographic recording. Two approaches are being investigated. In one, high-gain, signal-averaged ECG signals are being utilized to detect (delayed) depolarization potentials occurring during the repolarization process - an electrophysiologic state known to give rise to re-entrant arrhythmias. Initial results with this technique are encouraging. We have focused on another approach - that of body-surface potential mapping.(ABSTRACT TRUNCATED AT 250 WORDS)
Continuous pH Control in the Dyeing of Wool, Wool/Nylon and Wool/Acrylic Blends (1984)

Presented to the Society's West Riding Region on 14 October 1982 and reproduced from the German version first appearing in Textilveredlung, 17 (1982) 289. The acid and alkali binding power of wool is examined in the light of findings reported in the literature. Reference is made to the importance of pH as a key factor in the exhaust dyeing of wool. The optimised exhaust methods for dyeing wool and nylon proposed in the literature, especially those controlled by pH such as the Dosacid system (CGY), are enumerated. Dyeing trials with a new control and dispensing unit on stubbing and yarn at all stages of processing are described by means of a timet temperature I pH diagram. The economy of the Dosacid W system, which uses dilute sulphuric acid and caustic soda for all pH adjustments, is demonstrated by the chemical costs calculated for several batches.
Sams Teach Yourself HTML in 10 Minutes (2006)

1 What's It All About? 5 2 Creating Your First Page 11 3 Adding Text and More 19 4 Linking Text and Documents 32 5 Adding Your Own Style 39 6 Creating Lists 57 7 Creating Tables 64 8 Using Graphics 71 9 Mapping Images 82 10 Creating Frames 89 11 Building Online Forms 105 12 Making It Sing: Sound and Video 116 13 Designing with HTML 122 14 Creating Active Web Pages 134 15 Using Web Authoring Tools 142 16 Making a Name for Yourself 151 17 Planning for the Future 158 A HTML/XHTML Quick Reference 169 B Style Sheet Quick Reference 192 C Special Characters 205
Reactivity of Ethanediyl<i>S,S</i>-Acetals; 2. Synthesis of 2,3-Dihydro-1,4-dithiins (1991)

A new, reliable one-step synthesis of 2,3-dihydro-1,4-dithiins from ethanediyl S,S-acetal derivatives of carbonyl compounds is reported.
Assessment of umbilical arterial and venous flow using color Doppler (1999)

The estimates of fetal umbilical flow obtained by this Doppler method are consistent with previously published data. Averaging the arterial and venous flow is theoretically advantageous in reducing the inherent errors in estimating either the arterial or the venous flow. This method of measuring umbilical flow may have clinical potential in assessing fetal health and disease processes.
Effect of thrombomodulin on the kinetics of the interaction of thrombin with substrates and inhibitors (1986)

Thrombomodulin decreased by 20-30% the Michaelis constant of two tripeptidyl p-nitroanilide substrates of thrombin. Thrombomodulin increased the rate of inactivation of thrombin by two peptidyl chloromethane inhibitors by a similar amount. This effect appeared to be due to a decrease in the dissociation constants of the inhibitors. An improved method for the separation of fibrinopeptides A and B by h.p.l.c. was developed, and this method was used to study the effect of thrombomodulin on the thrombin-catalysed cleavage of fibrinogen. In this reaction, thrombomodulin was a competitive inhibitor with respect to the A alpha-chain of fibrinogen. The release of fibrinopeptide B was also inhibited by thrombomodulin. Analysis of the inhibition caused by thrombomodulin with respect to fibrinopeptides A and B yielded the same dissociation constant for the thrombin-thrombomodulin complex. In the presence of thrombomodulin, the rate of inactivation of thrombin by antithrombin III was stimulated 4-fold. This stimulation showed saturation kinetics with respect to thrombomodulin. Thrombomodulin was found to compete with hirudin for a binding site on thrombin. As a result of this competition, hirudin became a slow-binding inhibitor of thrombin at high thrombomodulin concentrations. Estimates of the dissociation constant for thrombomodulin were obtained in several of the above experiments, and the weighted mean value was 0.7 nM.
Gradient Channel Matrices for Orientation Estimation Report LiTH-ISY-R-2540 (2003)

Image intensity gradients can be encoded in a 2-dimensional channel representation. This report discusses the computation of such gradient channel matrices and what information can be extracted from it. In particular does this representation allow to distinguish multiple orientations and magnitudes in a single representation. It is shown that this can be used to recover orientation very accurately. This holds in particular near orientation discontinuities where classical orientation estimation fails.
Building the Future of Health Care on the Foundations of Family Practice (2000)

Hold on to your heritage as a family physician. It may save the health care system, or at least save your patient from it.
Laparo-endoscopic single-site (LESS) bilateral nephroureterectomy for patients with end-stage renal disease (2012)

We present the first report of LESS bilateral nephroureterectomy via the transperitoneal approach using the home-made single port. Two patients received LESS bilateral nephroureterectomies and bladder cuffs resection with homemade single ports, which were created by using an Alexis wound retractor as an access platform through a 4 cm incision. Distal ureters were resected through the same incision with Endo-loop. No additional ports were used and both procedures were completed successfully without traditional laparoscopic/open conversion or complication. LESS nephreoureterectomy with bladder cuff excision was performed in 460 and 635 minutes with an estimated blood loss of 50 and 400 mL, respectively. Patients were discharged on postoperative day 3 and 7, respectively. Our report demonstrates that LESS bilateral nephroureterectomy and bladder cuff resection is a safe and feasible procedure for urothelial carcinoma of upper urinary tract in patients at dialysis.
Scaffold Preparation and Parallel Synthesis of Arrays of 5,6,7,8‐Tetrahydropyrrolo‐azepinones in the Solution Phase (2008)

Abstract An efficient synthesis of a pyrrolo‐azepine scaffold for the parallel preparation of an array of (oxo‐pyrrolo‐azepinyl)acetamides is described. The Stetter cyclisation of 1,3‐cyclohexanedione with ethyl bromopyruvate was the key reaction in the assembly of a tetrahydrobenzofuran substrate which was submitted to a rapid transformation into a tetrahydroindole by microwave‐assisted cyclocondensation in the presence of glycine. The carbonyl group was then stereoselectively transformed into the corresponding ( Z )‐oxime which gave the pyrrolo‐azepinone by Beckmann rearrangement in the presence of polyphosphoric acid. Trimethylaluminium‐mediated amidation gave the corresponding amides which were finally N ‐alkylated at the 5‐position to give 52 diverse (pyrrolo‐azepinyl)acetamides, showing an appreciable exploration of the chemical space around the central heterocyclic core. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Pathological mechanisms in experimental autoimmune myasthenia gravis. II. Passive transfer of experimental autoimmune myasthenia gravis in rats with anti-acetylcholine recepotr antibodies. (1976)

Passive transfer of experimental autoimmune myasthenia gravis (EAMG) was achieved using the gamma globulin fraction and purified IgG from sera of rats immunized with Electrophus electricus (eel) acetylcholine receptor (AChR). This demonstrates the critical role of anti-AChR antibodies in impairing neuromuscular transmission in EAMG. Passive transfer of anti-AChR antibodies from rats with chronic EAMG induced signs of the acute phase of EAMG in normal recipient rats, including invasion of the motor end-plate region by mononuclear inflammatory cells. Clinical, eletrophysiological, histological, and biochemical signs of acute EAMG were observed by 24 h after antibody transfer. Recipient rats developed profound weakness and fatigability, and the posture characteristic of EAMG. Striking weight loss was attributable to dehydration. Recipient rats showed large decreases in amplitude of muscle responses to motor nerve stimulation, and repetitive nerve stimulation induced characteristic decrementing responses. End-plate potentials were not detectable in many muscle fibers, and the amplitudes of miniature end-plate potentials were reduced in the others. Passively transferred EAMG more severely affected the forearm muscles than diaphragm muscles, though neuromuscular transmission was impaired and curare sensitivity was increased in both muscles. Some AChR extracted from the muscles of rats with passively transferred EAMG was found to be complexed with antibody, and the total yield of AChR per rat was decreased. The quantitative decrease in AChR approximately paralleled in time the course of clinical and electrophysiological signs. The amount of AChR increased to normal levels and beyond at the time neuromuscular transmission was improving. The excess of AChR extractable from muscle as the serum antibody level decreased probably represented extrajunctional receptors formed in response to functional denervation caused by phagocytosis of the postsynaptic membrane by macrophages. The amount of antibody required to passively transfer EAMG was less than required to bind all AChR molecules in a rat's musculature. The effectiveness of samll amounts of antibody was probably amplified by the activation of complement and by the destruction of large areas of postsynaptic membrane by phagocytic cells. A self-sustaining autoimmune response to AChR was not provoked in animals with passively transferred EAMG.
Synthesis of KTiOPO <sub>4</sub> (KTP) Thin Films Using Metallo‐organics (1995)

Crack‐free and highly transparent KTiOPO 4 (KTP) thin films were synthesized by the sol‐gel method using a homogeneous precursor solution prepared from (“BuO) 2 ‐P(O)(OH), Ti(OEt) 4 , and KOEt in EtOH. Precipitated powders from the solution crystallized directly to KTP above 550°C. Polycrystalline KTP thin films were obtained at 600°C on various substrates. On NdAlO 3 (100) substrates, KTP films with (101) and (240) preferred orientations were formed at 600°C. KTP films on glass substrates showed a refractive index of 1.75 and an absorption edge of 350 nm. KTP films exhibited the second harmonic generation of the 532 nm light on irradiaton with 1064 nm light.
<i>Pseudomonas aeruginosa</i> Evasion of Phagocytosis Is Mediated by Loss of Swimming Motility and Is Independent of Flagellum Expression (2010)

Pseudomonas aeruginosa is a pathogenic Gram-negative bacterium that causes severe opportunistic infections in immunocompromised individuals; in particular, severity of infection with P. aeruginosa positively correlates with poor prognosis in cystic fibrosis (CF) patients. Establishment of chronic infection by this pathogen is associated with downregulation of flagellar expression and of other genes that regulate P. aeruginosa motility. The current paradigm is that loss of flagellar expression enables immune evasion by the bacteria due to loss of engagement by phagocytic receptors that recognize flagellar components and loss of immune activation through flagellin-mediated Toll-like receptor (TLR) signaling. In this work, we employ bacterial and mammalian genetic approaches to demonstrate that loss of motility, not the loss of the flagellum per se, is the critical factor in the development of resistance to phagocytosis by P. aeruginosa. We demonstrate that isogenic P. aeruginosa mutants deficient in flagellar function, but retaining an intact flagellum, are highly resistant to phagocytosis by both murine and human phagocytic cells at levels comparable to those of flagellum-deficient mutants. Furthermore, we show that loss of MyD88 signaling in murine phagocytes does not recapitulate the phagocytic deficit observed for either flagellum-deficient or motility-deficient P. aeruginosa mutants. Our data demonstrate that loss of bacterial motility confers a dramatic resistance to phagocytosis that is independent of both flagellar expression and TLR signaling. These findings provide an explanation for the well-documented observation of nonmotility in clinical P. aeruginosa isolates and for how this phenotype confers upon the bacteria an advantage in the context of immune evasion.
Supporting Context-Aware Cloud-Based Media Recommendations for Smartphones (2014)

The unprecedented advancements in broadband and mobile networks, the proliferation and the incredible appeal of smart devices such as smartphones, and the recent emergence of cloud computing are poised to drive the next generation of ubiquitous media delivery and consumption. As more media services become available, mobile users will waste invaluable time, seeking relevant media items. Therefore, to deliver relevant media services, with rich experience to mobile users, media service providers must consider the services that match user's contextual consumption choices. This paper proposes context-aware recommendation techniques to support the delivery of contextually relevant cloud-based media items to mobile users. The recommendation service works with a contextual user profile service, which relates user preferences to contexts in which such preferences are expressed, relying on a context recognition service, which identifies the user's dynamic contextual situation from smartphone built-in sensors. Experimental evaluations, using real world user and online movie data, established that the context-aware recommendation techniques are promising.
Interspecies Comparison Reveals Evolution of Control Regions in the Nematode Sex-Determining Gene <i>tra-2</i> (1996)

The Caenorhabditis elegans sex-determining gene tra-2 promotes female development and expresses 4.7-, 1.9- and 1.8-kb mRNAs. The 4.7-kb mRNA encodes the major feminizing activity of the locus, a predicted membrane receptor that mediates cell-to-cell communication, named TRA-2A. The tra-2 gene was characterized from a close relative, C. briggsae. The Cb-tra-2 gene expresses only a 4.7-kb mRNA and alternatively spliced variants, which encode TRA-2A homologues. The Cb-TRA-2A and Ce-TRA-2A sequences are highly diverged, sharing only 43% identity, although their hydropathy profiles remain remarkably similar. Three potential regulatory sites of Ce-tra-2 activity were previously identified by analyzing tra-2(eg), tra-2(gf), and tra-2(mx) mutations. Two of these sites, the EG site and MX region, are conserved in Cb-tra-2. By contrast, the two direct repeat elements in the Ce-tra-2 3' untranslated region, which are disrupted in tra-2(gf) mutants, are absent. Injection of Cb-tra-2 antisense RNA into C.briggsae mimics the Ce-tra-2 loss-of-function phenotype. Thus, antisense RNA permits studies of gene activity in nematodes that lack extensive genetics.