gebhart/patrepeval huggingface dataset:
Generation of Metastatic Variants by Transfection of a Nonmetastatic Rat Mammary Epithelial Cell Line with DNA from a Metastatic Rat Mammary Cell Line (1990)

Transfection of rat mammary (Rama) 37 epithelial cells, which yield nonmetastasizing adenomas in syngeneic Wistar-Furth rats, with HindIII-fragmented cellular DNA and the drug-resistance plasmids pSV2gpt or pSV2neo yields drug-resistant transformants with a frequency of 10(-4)-10(-5). Transformant cell lines transfected with the following, pSV2gpt alone, pSV2gpt and Rama 37 DNA, pSV2gpt and DNA from a metastasizing cell line Rama 800 (CT set), pSV2neo and salmon sperm DNA, pSV2neo and Rama 800 DNA (C set), all yield tumors when injected subcutaneously into syngeneic rats. A few transformants obtained by cotransfection with DNA from Rama 800 cells produce metastases in lungs and/or lymph nodes. The incidence of such metastases for two transfectants, termed CT4-41 and C18P, is significant at 20 and 24%, respectively, but only half (48%) that achieved with Rama 800 cells. Reintroduction into rats of cells cultured from a metastatic tumor of CT4-41 and of C18P, and from their lung or lymph node metastases, produces either a similar incidence (20-24%) or a significantly higher (48-52%) incidence of metastasis than that of the original transfectants. Cells cultured from nonmetastatic tumors fail to produce any metastatic lesions. When [32P]-labeled gpt or neo DNAs are hybridized to EcoRI-digested cellular DNA of the CT4-41 or C18P series of cell lines, tumors or metastases, gpt binds to one major fragment of 3,800 basepairs, and neo to two major fragments of 5,700 and 4,200 basepairs. The same cell lines produce hybridizing mRNAs of 1,500 and 1,900 bases for the CT4-41 series and 2,000-2,400 bases for the C18P series. It is suggested that transfection of DNA from the metastatic cells causes the nonmetastatic cells to become metastatic, in a genetically dominant manner, but additional steps are required for this process to become established and expressed at a level equivalent to that of the original, metastasizing donor cells.
ORESTE AND ORESTES (1969)

ORESTE AND ORESTES Get access PETER FRANCE PETER FRANCE SUSSEX Search for other works by this author on: Oxford Academic Google Scholar French Studies, Volume XXIII, Issue 2, April 1969, Pages 131–137, https://doi.org/10.1093/fs/XXIII.2.131 Published: 01 April 1969
Association Study between <i>Casein Kinase 1 Epsilon</i> Gene and Methamphetamine Dependence (2008)

Casein kinase 1 epsilon (CKIepsilon) is a component of the DARPP-32 in second-messenger pathway. CKIepsilon phosphorylates and activates DARPP-32, a key molecule in various complex signaling pathways, including dopamine and glutamine signaling, which have both been demonstrated to be main pathways in substance dependence. A recent clinical study showed that rs135745, a noncoding single nucleotide polymorphism of the 3'-untranslated region of the CSNK1E gene, was associated with the intensity of the subjective response to an oral amphetamine dose in normal volunteers. Differences in sensitivity to the drug should affect development of dependence to it. Hence, we genotyped rs135745 of the CSNK1E (MIM 600863) gene in 215 patients with methamphetamine dependence and 274 age- and gender-matched normal controls. No significant differences in genotype and allele frequencies were observed between the patients with methamphetamine dependence and controls. There was also no significant association between rs135745 and the clinical characteristics of methamphetamine dependence and co-morbid methamphetamine psychosis (e.g., age of first consumption, latency of psychosis, prognosis of psychosis after therapy, spontaneous relapse of psychotic symptoms, and poly-substance abuse status). The present findings suggest that having a genetic variant of the CSNK1E gene did not affect susceptibility to methamphetamine dependence or psychosis, at least in a Japanese population.
Dynamic Properties of Asphalt Concrete (1988)

Abstract This paper describes equipment developed to determine the dynamic properties of paving materials in axial and torsional loading. Dynamic properties were determined by the excitation of hollow cylindrical specimens using two independent sinusoidal loads with frequencies up to 30 Hz. An IBM PC/AT equipped with a Metrabyte DASH16 Data Acquisition Board was used to directly control two MTS Hydraulic Servorams. Menu-driven software was developed, taking advantage of DMA channels, so that data acquisition, waveform generation, and closed-loop control could simultaneously take place at rates above 5000 samples/s/channel. To convert raw data into graphs and parameters representative of dynamic material properties, a post-processing menu-driven program was also developed. Dynamic properties for a dense-graded asphalt concrete are presented for a range in temperatures from 11 to 40°C and for a range in frequencies from 0.5 to 20 Hz. Test results include: dynamic moduli in axial loading defined by |E*|, dynamic shear moduli defined by |G*|, internal damping determined in both axial and shear loading, and dynamic Poisson's ratios. Also included are results illustrating the effects of specimen density on these properties. Comparisons are included of stiffness moduli determined in a repeated pulse load test (resilient moduli) with those determined from the dynamic load equipment. The data also indicate that some care must be taken when comparing stiffness values determined by this type of loading with those determined from sinusoidal loading, particularly at higher temperatures. Differences in the stiffness moduli may result from the influence of the time between load applications (rest periods) on the value of the recoverable strain used to compute the stiffness (resilient modulus) in the repeated load test.
A novel echo-hiding scheme with backward and forward kernels (2003)

The paper presents a novel echo-hiding method for audio watermarking. The method is quite different from previous echo-hiding methods since it presents a new echo kernel which introduces a forward kernel as well as a backward kernel. The new kernel, a combination of the backward and forward kernels, can enhance considerably the watermark detection rate. Thus, it is possible to reduce echo amplitude. The paper proves mathematically that the combination of kernels improves watermark performance. Experimental results show that the proposed watermarking scheme is much better than previous echo-hiding schemes in terms of detection rate and imperceptibility.
An Intelligent System for Traffic Control in Smart Cities: A Case Study (2017)

Current traffic light systems use a fixed time delay for different traffic directions and do follow a particular cycle while switching from one signal to another. This creates unwanted congestion during peak hours, loss of man-hours and eventually decline in productivity. In addition to this, the current traffic light systems encourage extortion by corrupt traffic officials as commuters often violate traffic rules because of the insufficient time allocated to their lanes or may want to avoid a long waiting period for their lanes to come up. This research is aimed at tackling the afore-mentioned problems by adopting a density based traffic control approach using Jakpa Junction, one of the busiest junctions in Delta State, Nigeria as a case study. The developed system uses a microcontroller of PIC89C51 microcontroller duly interfaced with sensors. The signal timing changes automatically based on the traffic density at the junction, thereby, avoiding unnecessary waiting time at the junction. The sensors used in this project were infra-red (IR) sensors and photodiodes which were placed in a Line of Sight configuration across the loads to detect the density of the traffic signal. The density of the vehicles is measured in three zones i.e., low, medium and high based on which timings were allotted accordingly. The developed system has proven to be smart and intelligent and capable of curbing incidences of traffic malpractices and inefficiencies that have been the bane of current traffic congestion control systems in emerging cities of the third world.
40 ans de soudage et de traitement des matériaux par faisceau d'électrons (1998)

Les auteurs presentent une retrospective de 40 annees de soudage et de traitement des materiaux par faisceau d'electrons, depuis les premiers developpements jusqu'aux nombreuses applications industrielles actuelles. Les contributions en provenance de nombreux pays refletent les activites respectives de ces pays dans le domaine du faisceau d'electrons.
Composition of complex metal hydrides in polar solvents. I. Tertiary amines (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTComposition of complex metal hydrides in polar solvents. I. Tertiary aminesJ. A. Dilts and E. C. AshbyCite this: Inorg. Chem. 1970, 9, 4, 855–862Publication Date (Print):April 1, 1970Publication History Published online1 May 2002Published inissue 1 April 1970https://pubs.acs.org/doi/10.1021/ic50086a034https://doi.org/10.1021/ic50086a034research-articleACS PublicationsRequest reuse permissionsArticle Views272Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Simple Push-Pull Algorithm for Blue-Noise Sampling (2016)

We describe a simple push-pull optimization (PPO) algorithm for blue-noise sampling by enforcing spatial constraints on given point sets. Constraints can be a minimum distance between samples, a maximum distance between an arbitrary point and the nearest sample, and a maximum deviation of a sample's capacity (area of Voronoi cell) from the mean capacity. All of these constraints are based on the topology emerging from Delaunay triangulation, and they can be combined for improved sampling quality and efficiency. In addition, our algorithm offers flexibility for trading-off between different targets, such as noise and aliasing. We present several applications of the proposed algorithm, including anti-aliasing, stippling, and non-obtuse remeshing. Our experimental results illustrate the efficiency and the robustness of the proposed approach. Moreover, we demonstrate that our remeshing quality is superior to the current state-of-the-art approaches.
Activation of enteropathogenic <i>Escherichia coli</i> (EPEC) <i>LEE2</i> and <i>LEE3</i> operons by Ler (2000)

Enteropathogenic Escherichia coli (EPEC) produces attaching and effacing lesions (AE) on epithelial cells. The genes involved in the formation of the AE lesions are contained within a pathogenicity island named the locus of enterocyte effacement (LEE). The LEE comprises 41 open reading frames organized in five major operons: LEE1 , LEE2 , LEE3 , LEE4 and tir . The first gene of the LEE1 operon encodes a transcription activator of the other LEE operons that is called the LEE‐encoded regulator (Ler). The LEE2 and LEE3 operons are divergently transcribed with overlapping −10 promoter regions, and gene fusion studies have shown that they are both activated by Ler. Deletion analysis, using lacZ reporter fusions, of the LEE2 and LEE3 promoters demonstrated that deletions extending closer to the LEE2 transcription start site than −247 bp lead to loss of activation by Ler, whereas only 70 bp upstream of the LEE3 transcription start site is required for Ler‐mediated activation. We have purified Ler as a His‐tagged protein and used it to perform DNA‐binding assays with LEE2 and LEE3 . We observed that Ler bound to a DNA fragment containing the −300 to +1 region of LEE2; however, it failed to bind to a DNA fragment containing the −300 to +1 region of LEE3 , suggesting that Ler activates both operons by only binding to the regulatory region upstream of LEE2 . The Ler‐activatable LEE3::lacZ fusions extended to what would be −246 bp of the LEE2 operon. A lacZ fusion from the −300 to +1 region of LEE3 failed to be activated by Ler, consistent with our hypothesis that Ler activates the expression of LEE2 and LEE3 by binding to a region located downstream of the LEE3 transcription start site. DNase I footprinting revealed that Ler protected a region of 121 bp upstream of LEE2 . Purified Ler mutated in the coiled‐coil domain was unable to activate transcription and to bind to the LEE2 regulatory region. These data indicate that Ler may bind as a multimer to LEE2 and activate both divergent operons by a novel mechanism potentially involving changes in the DNA structure.
<title>Wavelet-based image compression and content authentication</title> (2001)

In the digital information age, digital content (audio, image, and video) can be easily copied, manipulated, and distributed. Copyright protection and content authentication of digital content has become an urgent problem to content owners and distributors. Digital watermarking has provided a valid solution to this problem. Based on its application scenario, most digital watermarking methods can be divided into two categories: robust watermarking and fragile watermarking. Here, we will concentrate on fragile watermarking of digital images, which is for image content authentication. Our fragile watermarking method is heavily based on the new image compression standard JPEG 2000. We choose a compressed bit stream from JPEG 2000 as the hash of an image, and embed the hash back to the image. The exceptional compression performance of JPEG 2000 solves the tradeoff between small hash size and high hash confidence level. In the authentication stage, the embedded compressed bit stream will be extracted. Then it will be compared with the compressed bit stream of the image to be authenticated. The authentication decision comes from the comparison result. Besides content authentication, we will also show how to employ this watermarking method for hiding one image into another.
The cytoprotective effect of Trolox demonstrated with three types of human cells (1990)

Trolox, a hydrophilic analogue of alpha-tocopherol, was reported to scavenge peroxyl radicals better than vitamin E in sodium dodecyl sulfate micelles and in liposomes. However, it was not known if Trolox protects human cells against oxyradical damage or if it acts as an antioxidant there. Here we demonstrate that Trolox prolonged substantially the survival of human ventricular myocytes and hepatocyte against oxyradicals generated with xanthine oxidase plus hypoxanthine, and prevented lysis of red cells exposed to an azo-initiator (2,2'-azo-bis(2-amidinopropane) HCl). Note that Trolox did not inhibit xanthine oxidase. In each cell type, the protection by Trolox was dose dependent and surpassed those given by such water-soluble antioxidants as ascorbic acid, superoxide dismutase, and (or) catalase, each examined at or near its optimal level in the same system. Using hepatocytes as a model, we further observed that Trolox reduced markedly the quantity of phospholipid conjugated dienes (a chemical imprint of oxyradical damage) in cells despite their exposure to oxyradicals. These data suggested that Trolox behaves as an antioxidant in cells as illustrated in hepatocytes.
Peptide Vaccines; New Approaches to Immunopotentiation (1995)

Once a candidate peptide has been identified or predicted then it must be delivered to the immune system in a suitable manner in order to elicit not just a high titre anti-peptide response but anti-peptide antibodies that will recognize and neutralize the infectious agent. Indeed, there has been a widely held view that, due to their relatively small molecular size, peptides are poor immunogens and thus require carrier-coupling to enhance their immunogenicity. As a result of this there are many examples of elegantly defined peptides which, having been coupled in an uncontrolled manner to large undefined carrier proteins, produced anti-peptide antibodies that totally failed to recognize the native protein.
Gadolinium DOTA Chelates Featuring Alkyne Groups Directly Grafted on the Tetraaza Macrocyclic Ring: Synthesis, Relaxation Properties, “Click” Reaction, and High-Relaxivity Micelles (2011)

This paper reports on the synthesis and relaxivity properties of tetraacetic DOTA-type chelating agents featuring one or two alkyne groups directly grafted on the tetraaza macrocyclic ring and available for “click” reactions with azide-bearing substrates. The racemic DOTAma ligand bearing one alkyne group was obtained by a bisaminal template route. The same approach was used to prepare ligand DOTAda substituted by two alkyne groups located on two adjacent carbon atoms. The S,S enantiomer of DOTAda was also prepared by a “crab-like” condensation. This ligand is the first example of a DOTA derivative featuring two reactive functions adjacent to each other on the macrocyclic ring. A triacetic monoalkyne ligand (DO3ma) was also synthesized for comparison purposes. NMR studies indicate that the Yb(III) chelates of DOTAma and DOTAda adopt two conformations in solutions in which the tetraaza ring is rigidified. The hydration state of the Eu(III) chelates was determined by luminescence spectroscopy, and the water exchange time of the Gd(III) complexes was measured by 17O NMR. Ring substitution accelerates the water exchange. These data were used to interpret nuclear magnetic relaxation dispersion curves of the Gd(III) chelates. Two long aliphatic chains have been added to DOTAda by a “click” procedure to form the (C18)2DOTAda ligand. The corresponding Gd(III) complex forms micelles of unusually high relaxivity presumably because of the close proximity of the aliphatic chains on the macrocyclic ring that ensures a rigid double anchoring into the micelles.
The MRN complex in double‐strand break repair and telomere maintenance (2010)

Genomes are subject to constant threat by damaging agents that generate DNA double-strand breaks (DSBs). The ends of linear chromosomes need to be protected from DNA damage recognition and end-joining, and this is achieved through protein-DNA complexes known as telomeres. The Mre11-Rad50-Nbs1 (MRN) complex plays important roles in detection and signaling of DSBs, as well as the repair pathways of homologous recombination (HR) and non-homologous end-joining (NHEJ). In addition, MRN associates with telomeres and contributes to their maintenance. Here, we provide an overview of MRN functions at DSBs, and examine its roles in telomere maintenance and dysfunction.
Surface and bulk acoustic waves in two-dimensional phononic crystal consisting of materials with general anisotropy (2004)

Successful application of photonic crystals has led recently to a rapidly growing interest in the analogous acoustic effects in periodic elastic structures called phononic crystals. This study is aimed at developing a theory for two-dimensional phononic crystal consisting of materials with general anisotropy. Explicit formulations of the plane harmonic bulk wave and the surface wave dispersion relations in such a general phononic structure are derived based on the plane wave expansion method. Two-dimensional phononic structures with either the square or the hexagonal lattice are considered in the numerical examples. Band gap characteristics of the phononic structures with different anisotropic background materials (isotropic, cubic, hexagonal, and orthorhombic) are calculated and discussed.
Bead-linked transposomes enable a normalization-free workflow for NGS library preparation (2018)

In contrast to solution-based library preparation, this bead-based technology produces a normalized, sequencing-ready library for a wide range of DNA input types and amounts, largely obviating the need for DNA quantitation. The robustness of this bead-based library preparation kit and flexibility of input DNA facilitates application across a wide range of fields.
Controlling the Edge Length of Gold Nanoprisms via a Seed‐Mediated Approach (2006)

Abstract A straightforward method is investigated for controlling and reinitiating the growth of single‐crystalline Au nanoprisms. This work is based on seeding methodology, and depends on the slow reduction of metal ions onto the surface of a growing nanoprism. In this manner, we can tailor the edge length of Au nanoprisms between 100 and 300 nm without changing their thickness or crystallinity. Each nanoprism size has been characterized by UV‐vis‐NIR (NIR: near‐IR) spectroscopy, transmission electron microscopy (TEM) techniques, and statistical analysis. Based on this work and existing silver halide crystal‐growth theories, a preliminary mechanism is proposed which comments on the interplay between crystal growth and surface chemistry that ultimately dictates the morphology of the resulting nanostructure.
Catalysis of 2- and 6- Substitution Reactions of Purines on Solid Phase (2001)

The synthesis of 2- and 6- disubstituted purines was accomplished using protic and Pd-mediated coupling reactions. Boronic acids and amines were successfully coupled under the same conditions.
Use of the 51Chromium Release Test to Demonstrate Antigenic Differences Between Extracellular and Intracellular Forms of Vaccinia Virus (1979)

Complement-dependent antibody lysis of vaccinia-infected cells was examined to demonstrate the antigenic differences between extracellular (ECV) and intracellular (ICV) forms of vaccinia virus. Cytolytic antibodies present in the antisera raised against ECV or live virus (LV) were completely removed by absorption with infected cell membranes or purified ECV but not with purified ICV. Absorption with infected cell membranes also abolished the neutralizing activity of ECV and LV antisera against ECV. On the other hand, antiserum against ICV did not contain cytolytic antibodies against vaccinia-infected cells, even though its neutralizing antibody titre against ICV was high. Moreover, both ECV and ICV antisera neutralized a small proportion of the heterologous form of virus, despite using purified preparations of ECV and ICV, respectively, for raising these antisera in rabbits. In contrast, the 51Cr release test only detected the antibodies against ECV and thus can be used to differentiate between the antibody activity of a serum against ECV and ICV.