gebhart/patrepeval huggingface dataset:
Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid and Real-Time Detection of Japanese Encephalitis Virus (2006)

The standardization and validation of a one-step, single-tube accelerated quantitative reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay is reported for rapid and real-time detection of Japanese encephalitis virus (JEV). The RT-LAMP assay reported in this study is very simple and rapid; the amplification can be obtained in 30 min under isothermal conditions at 63 degrees C by employing a set of six primers targeting the E gene of JEV. The RT-LAMP assay demonstrated exceptionally higher sensitivity compared to that of RT-PCR, with a detection limit of 0.1 PFU. The specificities of the selected primer sets were established by cross-reactivity studies with other closely related members of the JEV serocomplex as well as by evaluation of healthy human volunteers. The comparative evaluation of the RT-LAMP assay for clinical diagnosis with a limited number of patient cerebrospinal fluid samples revealed 85% concordance with conventional RT-PCR, with a sensitivity and a specificity of 100% and 86%, respectively. The concentration of virus in most of the clinical samples was 10(2) to 10(5) PFU/ml, as determined from the standard curve based on the time of positivity in the samples. In addition, the monitoring of gene amplification can also be visualized with the naked eye by using SYBR green I fluorescent dye. Thus, due to easy operation without a requirement of sophisticated equipment and skilled personnel, the RT-LAMP assay reported here is a valuable tool for the rapid and real-time detection of JEV not only by well-equipped laboratories but also by peripheral diagnostic laboratories with limited financial resources in developing countries.
Application of a Suitable Control Strategy for Grid-Connected Inverters to the Power Management of a Microgrid (2010)

Application of a Suitable Control Strategy for Grid-Connected Inverters to the Power Management of a Microgrid
A NOVEL REGION FILLING ALGORITHM FOR DISCONTINUOUS CONTOURS (2017)

This is a new algorithm which can be applied to contours having discontinuous boundaries in order to obtain its1-pixel thickness equivalent and complete the shape of the contour which can be followed by region filling operation. The conventional thinning algorithm often modifies the shape of the contour and the obtained skeleton will not resemble the original image and hence the thinning operation is not used in the proposed algorithm.
Using the Web and Jini to link vending machines and enterprise systems (2002)

The use of open technologies in enterprise resource planning (ERP) systems and the development of new distributed infrastructures allows for novel systems to be developed. This paper reports on a project to bring vending machines into the scope of ERP systems, using Jini for network discovery and event generation.
The role of double hydrogen bonds in asymmetric direct aldol reactions catalyzed by amino amide derivatives (2010)

The double hydrogen bonding activation of carbonyl functionality has been a general strategy for the design of amino amide organocatalysts for highly enantioselective direct aldol reactions of various ketones with aldehydes conducted in either organic solvents or aqueous media. Moreover, this concept may suggest an activation mode to create new catalysts for other related asymmetric transformations.
Reliability of NAND Flash Memories: Planar Cells and Emerging Issues in 3D Devices (2017)

We review the state-of-the-art in the understanding of planar NAND Flash memory reliability and discuss how the recent move to three-dimensional (3D) devices has affected this field. Particular emphasis is placed on mechanisms developing along the lifetime of the memory array, as opposed to time-zero or technological issues, and the viewpoint is focused on the understanding of the root causes. The impressive amount of published work demonstrates that Flash reliability is a complex yet well-understood field, where nonetheless tighter and tighter constraints are set by device scaling. Three-dimensional NAND have offset the traditional scaling scenario, leading to an improvement in performance and reliability while raising new issues to be dealt with, determined by the newer and more complex cell and array architectures as well as operation modes. A thorough understanding of the complex phenomena involved in the operation and reliability of NAND cells remains vital for the development of future technology nodes.
Feature correspondence and deformable object matching via agglomerative correspondence clustering (2009)

We present an efficient method for feature correspondence and object-based image matching, which exploits both photometric similarity and pairwise geometric consistency from local invariant features. We formulate object-based image matching as an unsupervised multi-class clustering problem on a set of candidate feature matches, and propose a novel pairwise dissimilarity measure and a robust linkage model in the framework of hierarchical agglomerative clustering. The algorithm handles significant amount of outliers and deformation as well as multiple clusters, thus enabling simultaneous feature matching and clustering from real-world image pairs with significant clutter and multiple deformable objects. The experimental evaluation on feature correspondence, object recognition, and object-based image matching demonstrates that our method is robust to both outliers and deformation, and applicable to a wide range of image matching problems.
PI3K-Akt Pathway Suppresses Coagulation and Inflammation in Endotoxemic Mice (2004)

These data suggest that the PI3K-Akt pathway suppresses LPS-induced inflammation and coagulation in endotoxemic mice.
Associations between Methylation of Paternally Expressed Gene 3 (PEG3), Cervical Intraepithelial Neoplasia and Invasive Cervical Cancer (2013)

Cytology-based screening for invasive cervical cancer (ICC) lacks sensitivity and specificity to discriminate between cervical intraepithelial neoplasia (CIN) likely to persist or progress from cases likely to resolve. Genome-wide approaches have been used to identify DNA methylation marks associated with CIN persistence or progression. However, associations between DNA methylation marks and CIN or ICC remain weak and inconsistent. Between 2008-2009, we conducted a hospital-based, case-control study among 213 Tanzania women with CIN 1/2/3 or ICC. We collected questionnaire data, biopsies, peripheral blood, cervical scrapes, Human papillomavirus (HPV) and HIV-1 infection status. We assessed PEG3 methylation status by bisulfite pyrosequencing. Multinomial logistic regression was used to estimate odds ratios (OR) and confidence intervals (CI 95%) for associations between PEG3 methylation status and CIN or ICC. After adjusting for age, gravidity, hormonal contraceptive use and HPV infection, a 5% increase in PEG3 DNA methylation was associated with increased risk for ICC (OR = 1.6; 95% CI 1.2-2.1). HPV infection was associated with a higher risk of CIN1-3 (OR = 15.7; 95% CI 5.7-48.6) and ICC (OR = 29.5, 95% CI 6.3-38.4). Infection with high risk HPV was correlated with mean PEG3 differentially methylated regions (DMRs) methylation (r = 0.34 p<0.0001), while the correlation with low risk HPV infection was weaker (r = 0.16 p = 0.047). Although small sample size limits inference, these data support that PEG3 methylation status has potential as a molecular target for inclusion in CIN screening to improve prediction of progression. Impact statement: We present the first evidence that aberrant methylation of the PEG3 DMR is an important co-factor in the development of Invasive cervical carcinoma (ICC), especially among women infected with high risk HPV. Our results show that a five percent increase in DNA methylation of PEG3 is associated with a 1.6-fold increase ICC risk. Suggesting PEG3 methylation status may be useful as a molecular marker for CIN screening to improve prediction of cases likely to progress.
Intestinal Microbiota Promote Enteric Virus Replication and Systemic Pathogenesis (2011)

Intestinal bacteria aid host health and limit bacterial pathogen colonization. However, the influence of bacteria on enteric viruses is largely unknown. We depleted the intestinal microbiota of mice with antibiotics before inoculation with poliovirus, an enteric virus. Antibiotic-treated mice were less susceptible to poliovirus disease and supported minimal viral replication in the intestine. Exposure to bacteria or their N-acetylglucosamine-containing surface polysaccharides, including lipopolysaccharide and peptidoglycan, enhanced poliovirus infectivity. We found that poliovirus binds lipopolysaccharide, and exposure of poliovirus to bacteria enhanced host cell association and infection. The pathogenesis of reovirus, an unrelated enteric virus, also was more severe in the presence of intestinal microbes. These results suggest that antibiotic-mediated microbiota depletion diminishes enteric virus infection and that enteric viruses exploit intestinal microbes for replication and transmission.
New stereoselective synthesis of 9-methyl-cis-decalin derivatives by double Michael reaction of 3-methyl-4-methylenecyclohex-2-enone and its congeners with dimethyl 3-oxoglutarate (1982)

Double Michael reaction of 3-methyl-, 2,3-dimethyl-, and 3,5-dimethyl-4-methylenecyclohex-2-enone with dimethyl 3-oxoglutarate in dimethyl sulphoxide in the presence of potassium fluoride gave stereoselectively 9-methyl-, 1,9-dimethyl-, and 4,9-dimethyl-6,8-dimethoxycarbonyl-cis-decahydronaphthalene-2,7-dione, respectively, and starting from these annulation products some sesquiterpenes having the cis-decahydronaphthalene skeleton were formally synthesised.
The Passing of the Seasons and the Apocalyptic in "Sir Gawain and the Green Knight" (1986)

Sir Gawain and Green Knight' is a poem which would, at glance seem to have little in common with three overtly religious poems, especially Purity, to which it is linked in terms of style and language. Yet seemingly secular Sir Gawain and Green Knight is surprisingly at one with other works ascribed to anonymous fourteenth-century Gawain-Poet. The thematic signature that stamps Sir Gawain, as well as other poems, proves to be poet's preoccupation with judging communities of men with which he deals in order to effect separation of saved from damned.2 Sir Gawain is, after all, a poem about moral assessments and values, and its web of themes and imagery can be seen to be centered directly upon problem of judgment,3 where thematic focus is consistently on ends of things and how those ends relate to their beginnings. This should come as no surprise, since poem's famous excursus detailing passing of year-a passage used to join initiation of adventure with onset of its completion-is immediately preceded by a narrative intrusion in which poet muses on unexpected ends of endeavors begun by men in a mutable world: A 3ere 3ernes ful 3erne, and 3eldes neuer lyke, / be forme to be be fynisment foldez ful selden (498-499). That ends are frequently belied by beginnings is of utmost importance to our understanding of portrait of this seemingly vibrant court whose members are in their first age (54), painted by an artist whose frequent theme is that the shall be last.4
Temporary Vena Caval Filtration Preliminary Clinical Experience With Removable Vena Caval Filters (1999)

Removable vena caval devices look attractive in order to prevent pulmonary embolism (PE) in high risk patients when anticoagulant therapy has to be discontinued for periods shorter than 14 days. We describe our preliminary experience about 10 consecutive patients. All patients were anticoagulated for recent venous thromboembolism and the indication of vena caval filtration was mainly a short-term contraindication to anticoagulation due to surgery (8 cases) or delivery (1 case). Gunther Tulip retrievable vena caval device was used. No clinical manifestation of PE occurred during the filtration period. No thrombosis was detected at the insertion site, nor was any filter thrombosis found at the time of retrieval. Eight out of ten filters were easily retrieved through internal jugular vein. In one patient, filter could not be removed due to device tilting; in two patients, permanent filtration was secondarily requested. These results suggest that temporary vena caval filtration is efficient and safe for preventing pulmonary embolism during a short-term discontinuation of anticoagulant therapy in high risk patients.
Multipath propagation model for line-of-sight street microcells in urban areas (2002)

A multipath propagation model and a prediction equation for r.m.s. delay spread are discussed for LOS street microcells with building roof base sites in urban areas. The proposed model is established to explain a mechanism of multipath propagation. The delay profiles calculated using the model agreed well with the measured profiles. The factors influencing the r.m.s. delay spread are investigated based on the model, then it is found that the factors are LOS and road width, etc. The prediction equation for medium r.m.s. delay spread based on those factors is proposed.
Enhanced resolution of proton NMR spectra of malignant lymph nodes using magic‐angle spinning (1996)

Proton NMR spectroscopy has proven useful in the detection of cancer in lymph node tissue. However, due to the high fat content of this type of tissue, 2D 1H COSY measurements (requiring acquisition times of 4-5 h or longer) are necessary to obtain the spectral information necessary for diagnosis. T2-filtered proton magic-angle spinning (MAS) NMR spectroscopy provides 1D spectra of lymph nodes in approximately 20 min with sufficient spectral resolution allowing for identification of changes in cellular chemistry due to the presence of malignant cells. MAS data from lymph nodes of five control and six rats with mammary adenocarcinoma (R13762) demonstrated increases in the signal intensity of resonances associated primarily with lactate (delta = 4.12 ppm) P < 0.0004, creatines/lysine (delta = 3.04 ppm) P < 0.0032, and glutamate/ glutamine (delta = 2.36 ppm) P < 0.0002 in metastatic compared with normal lymph nodes. The infiltration of lymph nodes by malignant cells is an important prognostic factor for many cancers. The rapid assessment of node tissue without the introduction of sampling errors (inherent in currently employed histological procedures) would allow postoperative therapy decisions to be made more efficiently.
Using Random Quasi-Monte-Carlo Within Particle Filters, With Application to Financial Time Series (2005)

This article presents a new particle filter algorithm which uses random quasi-Monte-Carlo to propagate particles. The filter can be used generally, but here it is shown that for one-dimensional state-space models, if the number of particles is N, then the rate of convergence of this algorithm is N−1. This compares favorably with the N−1/2 convergence rate of standard particle filters. The computational complexity of the new filter is quadratic in the number of particles, as opposed to the linear computational complexity of standard methods. I demonstrate the new filter on two important financial time series models, an ARCH model and a stochastic volatility model. Simulation studies show that for fixed CPU time, the new filter can be orders of magnitude more accurate than existing particle filters. The new filter is particularly efficient at estimating smooth functions of the states, where empirical rates of convergence are N−3/2; and for performing smoothing, where both the new and existing filters have the same computational complexity.
Solvent-free synthesis and structural characterization of azolyl-substituted pyrimidines (2002)

Base-catalyzed trimerization of N-cyanomethylazoles under pressure and in solvent-free conditions afforded 4-amino-2,6-bis(azol-1-ylmethyl)-5-(azol-1-yl)pyrimidines (1–3) in 33–67% yields. The structures of these compounds was determined by a combination of NMR techniques and X-ray crystallography. The 4-amino groups show a restricted rotation around the C–N bond; the free energy of activation for this process was determined by variable temperature experiments. In the crystal structure of the pyrazol-1-yl derivative, the amino group shows a distorted planar geometry in both independent molecules and acts as a double hydrogen bond donor towards two of the three pyrazole rings, forming ribbons of R22(10) rings.
Broadband quantum well infrared photodetectors (1999)

Typical quantum well infrared photodetectors (QWIPs) exhibit rather narrow spectral bandwidth of 1 to 2 micrometer. For certain applications, such as spectroscopy, sensing of a broader range of infrared radiation is highly desirable. In this work, we report the design of five broadband (BB-) QWIPs sensitive over the 7 to 14 micrometer spectral range. Three n- type BB-QWIPs consisting of three, four, and five quantum wells of different thickness and/or composition in a unit cell, which are then repeated 20 times for the three and four quantum wells (QW) devices and 3 times for the five QWs device to create the BB-QWIP structures, are demonstrated. The three- well n-type In<SUB>x</SUB>Ga<SUB>1-x</SUB>As/Al<SUB>y</SUB>Ga<SUB>1-y</SUB>As BB-QWIP is designed to have a response peak at 10 micrometer, with a FWHM bandwidth that varies with the applied bias. A maximum bandwidth of (Delta) (lambda) /(lambda) <SUB>p</SUB> equals 21% was obtained for this device at V<SUB>b</SUB> equals -2 V. The four- well n-type In<SUB>x</SUB>Ga<SUB>1-x</SUB>As/GaAs BB-QWIP not only exhibits a very large responsivity of 2.31 A/W at 10.3 micrometer and V<SUB>b</SUB> equals +4.5 V, but also achieves a broader bandwidth of (Delta) (lambda) /(lambda) <SUB>p</SUB> equals 29% than the three-well device. The five-well n-type In<SUB>x</SUB>Ga<SUB>1- x</SUB>As/GaAs BB-QWIP has achieved a FWHM bandwidth of (Delta) (lambda) /(lambda) <SUB>p</SUB> equals 28% at V<SUB>b</SUB> equals 1.75 V. In addition, two p-type In<SUB>x</SUB>Ga<SUB>1-x</SUB>As/GaAs BB-QWIPs with variable well thickness and composition, sensitive in the 7 - 14 micrometer spectral range, are also demonstrated. The variable composition p-type BB-QWIP has a very large FWHM bandwidth of (Delta) (lambda) /(lambda) <SUB>p</SUB> equals 48% at V<SUB>b</SUB> equals -1.5 V and T equals 40 K. The variable thickness p- type BB-QWIP was found to have an even broader FWHM bandwidth of (Delta) (lambda) /(lambda) <SUB>p</SUB> equals 63% at V<SUB>b</SUB> equals 1.1 V and T equals 40 K, with a corresponding peak responsivity of 25 mA/W at 10.2 micrometer. The results reveal that p-type BB- QWIPs have a broader and flatter spectral bandwidth but lower responsivity than that of n-type BB-QWIPs under similar operating conditions.
[The activity of alpha-2 macroglobulin in the blood of patients with prostatic carcinoma]. (1997)

The activity of alpha 2-macroglobulin in the blood of the patients with prostatic carcinoma (PC) and benign prostatic hyperplasia (BPH) was examined. It was shown an decrease of alpha 2-macroglobulin in patients with prostatic carcinoma in comparison with control group consisted of the patients with benign prostatic hyperplasia. The low activity of alpha 2-macroglobulin in the blood od the patients with PC can be a symptom of an increase risk of haemorrhagic complications.
The bis(β‐Diketonato)Platinum(II) Complexes (1980)

This chapter contains sections titled: Preparation of Stock Solution Bis[2,4-Pentanedionato(1-)]platinum (II) Bis[1,1,1-trifluoro-2,4-pentanedionato(1-)]platinum(II) Bis[1,1,1,5,5,5-Hexafluoro-2,4-pentanedionato(1-)]platinum(II)
Clinical relevance of pulse pressure variations for predicting fluid responsiveness in mechanically ventilated intensive care unit patients: the grey zone approach (2014)

In ventilated ICU patients, PPV values between 4 and 17%, encountered in 62% patients exhibiting validity prerequisites, did not predict fluid responsiveness.
An Improved Technic for Vascular Isolation of the Liver (1966)

Heaney, John P. M.D.; Stanton, William K. M.D.; Halbert, David S. M.D.; Seidel, Joshua M.D.; Vice, Tom D.V.M. Author Information
CELLULAR ENZYME-LINKED IMMUNOSPECIFIC ASSAY (CELISA) (1983)

The cellular enzyme-linked immunospecific assay (CELISA) quantitates the binding of antibody to cell-surface antigens. When conditions that produced the maximum specific enzymatic activity of the conjugate were used, the CELISA was able to screen hybridoma supernatant fluids for polymorphic anti-HLA monoclonal antibodies using as few as 20,000 normal human peripheral blood mononuclear target cells per well. The CELISA detected as little as 24 pg (0.55 fM or 330 X 10(6) molecules) of cell-surface HLA alloantigen and as little as 40 pg of monoclonal antibody bound to cell-surface antigen. In conjunction with various monoclonal antibodies to human peripheral blood mononuclear cell differentiation antigens, the CELISA was used to quantitate levels of different peripheral blood mononuclear cell subsets in normal and diseased people objectively, thus providing a simple, sensitive, and inexpensive alternative to immunofluorescence microscopic and flow cytometric techniques normally used to phenotype peripheral blood mononuclear cells.
Blur identification using bispectrum (2002)

Blur identification methods based on the bispectrum of an observed noisy and blurred image are proposed. Blur identification is addressed, in the case of uniform blurs, by zero-crossing detection in a particular cross section of the bispectrum of the observed image. The identification of general finite-impulse-response blurs is considered. The blurred image is represented by a nonminimum phase ARMA (autoregressive moving average) model whose parameters are identified using the complex bispectrum of the observed image. These methods are superior to previous methods which are based on the second-order statistics of the image, since the bispectrum is insensitive to additive, Gaussian noise in theory, and it contains the phase of the blur transfer function. Simulation results are provided.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Design and Implementation for Management System of Opening Computer Laboratory (2013)

As for university computer laboratory,management is cumbersome and inefficient.Through comparison study,based on unmanned management and idea of being open all day long,VB.NET language is designed and developed as a management system of opening computer laboratory with four layer system structures.RF card is used as the basic media in this system,realizing the real-time,open,unmanned management on all computers in the laboratory through information interaction between client and server.The use of the system reduces the personnel,makes it convenient for users,and improves the teaching quality and management level.
Mechanical Properties of Liquid‐Phase‐Bonded Copper‐Ceramic Substrates (1982)

Liquid‐phase bonding of copper foils to ceramic substrates is a very attractive technique for the fabrication of metallized substrates for hybrid power electronics applications. The design and reliability considerations of such substrates necessitate the knowledge of their mechanical properties. Therefore, stresses and strains in the copper and in the ceramic of bonded substrates were investigated. Theoretical calculations are presented to support the experimental results and to find the influence of stresses and strains in the fabrication of copper ceramic substrates using the liquid‐phase‐bonding technique.