gebhart/patrepeval huggingface dataset:
Stimulation of Nicotine Demethylation by NaHCO<sub>3</sub> Treatment Using Greenhouse-Grown Burley Tobacco (2003)

Experiments were conducted in tobacco (Nicotiana tabacum L.) to investigate the effect of sodium bicarbonate (NaHCO(3)) on the conversion of nicotine to nornicotine, a secondary alkaloid that can form the tobacco-specific nitrosamine N-nitrosonornicotine (NNN). The results showed that, under optimum conditions, NaHCO(3) stimulated nicotine conversion in converter plants to the maximum level predetermined by the genetic background. The conversion level in NaHCO(3)-treated leaves was 2-3 times higher than that in control leaves. For young seedlings the optimum concentration of sodium bicarbonate was 0.8% aqueous solution, and for adult plants the optimum concentration was 1%. Lower concentrations resulted in partial stimulation, whereas higher concentration damaged leaf tissue and resulted in a lower conversion level. Studies with different temperatures (from 22 to 43 degrees C) showed that 37 degrees C was optimal. This temperature allowed the least amount of time, 2-3 days for mature leaves and 4-6 days for green leaves, for the major converters to reach >95% of nicotine conversion. An examination of leaves from different growth stages and stalk positions showed that the amount of time needed for conversion was longer for young leaves and shorter for mature leaves. Treatment of leaves with NaHCO(3) affords a rapid and convenient means of identifying and removing nornicotine converter plants during growth in the greenhouse or field.
Acute transfusion-related lung injury (TRALI) in pregnancy.Case report and bibliographic review (2020)

BACKGROUD. Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress caused by blood transfusion. The Food and Drug Administration
(FDA) places TRALI as the main cause of death associated with transfusion.
OBJECTIVE. The present clinical case is reported, treated in a Third Level Hospital and a search was carried out to update the topic on the management of TRALI
in a pregnant patient.
CLINICAL CASE. A 35-year-old woman with a pregnancy of 33.2 weeks of gestation, placental accreta, with a threat of preterm delivery and severe anemia
that required hemotransfusion of erythrocyte concentrate, subsequently with a respiratory distress syndrome clinic. TRALI was diagnosed, it was managed in
intensive therapy with diuretic and inotropic, it evolved clinically to the improvement, until its total improvement, Placental accreta was corroborated, cystoscopy
was performed without data on bladder invasion. Subsequently, he presented uterine activity and premature rupture of membranes, caesarean section and obstetric
hysterectomy were performed. The patient evolved satisfactorily and left without incident.
CONCLUSIONS There are very few documented reports of TRALI in the period prior to delivery. Pregnant women are disadvantaged by the physiological changes
of pregnancy such as decreased residual volume and respiratory reserve capacity, decreased capillary colidosidotic pressure. Therefore, the diagnosis of TRALI is
important to manage timely management. The management of respiratory failure in TRALI must be done multidisciplinary way.
KEY WORDS: TRALI, Acute lung injury, transfusion, pregnancy, transfusion adverse reactions.
Novel Protein Kinases Associated with Calcineurin B–like Calcium Sensors in Arabidopsis (1999)

Members of the Arabidopsis calcineurin B-like Ca(2)+ binding protein (AtCBL) family are differentially regulated by stress conditions. One AtCBL plays a role in salt stress; another is implicated in response to other stress signals, including drought, cold, and wounding. In this study, we identified a group of novel protein kinases specifically associated with AtCBL-type Ca(2)+ sensors. In addition to a typical protein kinase domain, they all contain a unique C-terminal region that is both required and sufficient for interaction with the AtCBL-type but not calmodulin-type Ca(2)+ binding proteins from plants. Interactions between the kinases and AtCBLs require micromolar concentrations of Ca(2)+, suggesting that increases in cellular Ca(2)+ concentrations may trigger the formation of AtCBL-kinase complexes in vivo. Unlike most serine/threonine kinases, the AtCBL-interacting kinase efficiently uses Mn(2)+ to Mg(2)+ as a cofactor and may function as a Mn(2)+ binding protein in the cell. These findings link a new type of Ca(2)+ sensors to a group of novel protein kinases, providing the molecular basis for a unique Ca(2)+ signaling machinery in plant cells.
Effects of borehole stress concentration on elastic wave velocities in sandstones (2006)

The stress redistribution that occurs in the vicinity of a borehole may lead to damage or failure of the rock. Recent developments in sonic logging allow the variation in elastic wave velocities around a borehole to be investigated. Since elastic wave velocities in sandstones are sensitive to changes in stress due to the presence of stress‐sensitive grain boundaries within the rock, this allows the changes in stress to be monitored. For small changes in stress, perturbation theory shows that a radially polarized, vertically propagating shear wave is more sensitive to radial stress than to hoop stress. Close to the wellbore large changes in stress occur. Non‐linearity in the variation in velocity with stress increases the sensitivity of wave velocities to the wellbore pressure, and can cause the velocity of both compressional and shear waves to be significantly lower than that predicted by perturbation theory.
Fast video target tracking in the presence of occlusion and camera motion blur (2007)

This paper addresses the issue of tracking partially occluded targets in videos recorded by moving cameras of either handhold or airborne. We propose a fast geometric constraint global motion algorithm to reduce the computation overhead dramatically and the effect caused by outliers from moving targets. A recursive least-squares filter with forgetting factor is utilized to filter out disturbances and to provide a better estimation of the target's position in the current frame as well as the prediction of the position and velocity for the next frame. The filter uses the affine model and the primary search result to construct a kinetic model. After that, a compact search region is formed based on the prediction to reduce mismatch and improve computation speed. The adaptive template matching is applied to improve the performance further. With these important steps, a tracking algorithm is developed and tested on real video sequences.
A high resolution, electrostatically-driven commercial inkjet head (2002)

A fully-micromachined, low power, large nozzle count and high resolution "electrostatically actuated inkjet (SEAJet/sup TM/)" head has been developed for a POS printer. As 3D multiple-step structured nozzles are required for straight and high frequency ink ejection, it was formed by ICP deep-RIE employing a "multiple-step mask method" which makes troublesome stereo-photolithography unnecessary and 128 nozzles/chip were fabricated simultaneously. The required thin, 2 /spl mu/m-thick pressure plates were formed by B doped etch-stop technology combined with two-step alkaline etching which enables smooth-surfaced and uniform (2.15/spl plusmn/0.35 (3 /spl sigma/)/spl mu/m) pressure plates. The typical driving voltage is 26.5 V and the SEAJet head has achieved the uniform ink ejection up to a driving frequency of 18 kHz. The life of the actuator has been confirmed to be more than 4 billion times actuation. The typical printing speed of the POS printer is 15 1/s (lines per second) for a rolled paper and 3 ppm (papers per min.) for A4 paper in 360 dpi (dots per inch) high resolution, a performance level that makes this the fastest inkjet POS printer in the world. The average power consumption was measured as only 0.525 mW/nozzle. It is only one-thousandth of that of a typical thermal inkjet.
Potent inhibition of scrapie prion replication in cultured cells by bis-acridines (2003)

Prion diseases are characterized by an accumulation of PrP(Sc), a misfolded isoform of the normal cellular prion protein, PrP(C). We previously reported the bioactivity of acridine-based compounds against PrP(Sc) replication in scrapie-infected neuroblastoma cells and now report the improved potency of bis-acridine compounds. Bis-acridines are characterized by a dimeric motif, comprising two acridine heterocycles tethered by a linker. A library of bis-(6-chloro-2-methoxy-acridin-9-yl) and bis-(7-chloro-2-methoxy-benzo[b][1,5]naphthyridin-10-yl) analogs was synthesized to explore the effect of structurally diverse linkers on PrP(Sc) replication in scrapie-infected neuroblastoma cells. Structure-activity analysis revealed that linker length and structure are important determinants for inhibition of prion replication in cultured scrapied cells. Three bis-acridine analogs, (6-chloro-2-methoxy-acridin-9-yl)-(3-[4-[3-(6-chloro-2-methoxy-acridin-9-ylamino)-propyl]-piperazin-1-yl]-propyl)-amine, N,N'-bis-(6-chloro-2-methoxy-acridin-9-yl)-1,8-diamino-3,6-dioxaoctane, and (1-[[4-(6-chloro-2-methoxy-acridin-9-ylamino)-butyl]-[3-(6-chloro-2-methoxy-acridin-9-ylamino)-propyl]-carbamoyl]-ethyl)-carbamic acid tert-butyl ester, showed half-maximal inhibition of PrP(Sc) formation at 40, 25, and 30 nM, respectively, and were not cytotoxic to uninfected neuroblastoma cells at concentrations of 500 nM. Our data suggest that bis-acridine analogs may provide a potent alternative to the acridine-based compound quinacrine, which is currently under clinical evaluation for the treatment of prion disease.
A major quantitative trait locus on chromosome 3 controls colitis severity in IL-10-deficient mice (2001)

Colitic lesions are much more severe in C3H/HeJBir (C3H) than C57BL/6J (B6) mice after 10 backcrosses of a disrupted interleukin-10 (Il10) gene. This study identified cytokine deficiency-induced colitis susceptibility (Cdcs) modifiers by using quantitative trait locus (QTL) analysis. A segregating F(2) population (n = 408) of IL-10-deficient mice was genotyped and necropsied at 6 weeks of age. A major C3H-derived colitogenic QTL (Cdcs1) on chromosome (Chr.) 3 contributed to lesions in both cecum [logarithm of odds ratio (LOD) = 14.6)] and colon (LOD = 26.5) as well as colitis-related phenotypes such as spleen/body weight ratio, mesenteric lymph node/body weight ratio, and secretory IgA levels. Evidence for other C3H QTL on Chr. 1 (Cdcs2) and Chr. 2 (Cdcs3) was obtained. Cdcs1 interacted epistatically or contributed additively with loci on other chromosomes. The resistant B6 background also contributed colitogenic QTL: Cdcs4 (Chr. 8), Cdcs5 (Chr. 17, MHC), and Cdcs6 (Chr. 18). Epistatic interactions between B6 QTL on Chr. 8 and 18 contributing to cecum hyperplasia were particularly striking. In conclusion, a colitogenic susceptibility QTL on Chr. 3 has been shown to exacerbate colitis in combination with modifiers contributed from both parental genomes. The complex nature of interactions among loci in this mouse model system, coupled with separate deleterious contributions from both parental strains, illustrates why detection of human inflammatory bowel disease linkages has proven to be so difficult. A human ortholog of the Chr. 3 QTL, if one exists, would map to Chr. 4q or 1p.
FOOTPRINT, Issue # 16 | Spring 2015 | Commoning as Differentiated Publicness (2015)

2014-2015 > Academic research: refereed > Publication in refereed journal
The potential of Ultrogel®, an agarose‐polyacrylamide copolymer, as a matrix for affinity chromatography (1976)

The selection of the inert support in the synthesis of adsorbents for affinity chromatography is constrain, ed by several factors. It should be stable to chemical and biological degradations, hydrophilic, easily substituted and uniform to enhance the chromatographic process. The comparative advantages of polyacrylamide, glass, cellulose, cross-linked dextrans and beaded agarose have been reviewed [1-3] ; currently the most popular support materials are polysaccharides, especially beaded agarose.
BIPARTITE STRUCTURE OF THE <i>ade3</i> LOCUS OF <i>SACCHAROMYCES CEREVISIAE</i> (1977)

Forty ade3 mutants were examined with respect to their growth requirements, levels of the tetrahydrofolate interconversion enzymes, and/or map positions. Four deletions were detected. Mutations that result in a requirement for adenine and histidine map in one region of the locus; those which result in a requirement for adenine only map in a quite separate region of the locus, a region not disclosed in previous studies. No correlation was observed between growth properties of the strains and enzyme levels.
The Epidemic of Extended-Spectrum-β-Lactamase-Producing Escherichia coli ST131 Is Driven by a Single Highly Pathogenic Subclone, <i>H</i> 30-Rx (2013)

We applied an advanced genomic approach to study the recent evolutionary history of one of the most important Escherichia coli strains in circulation today. This strain, called sequence type 131 (ST131), causes multidrug-resistant bladder, kidney, and bloodstream infections around the world. The rising prevalence of antibiotic resistance in E. coli is making these infections more difficult to treat and is leading to increased mortality. Past studies suggested that many different ST131 strains gained resistance to extended-spectrum cephalosporins independently. In contrast, our research indicates that most extended-spectrum-cephalosporin-resistant ST131 strains belong to a single highly pathogenic subclone, called H30-Rx. The clonal nature of H30-Rx may provide opportunities for vaccine or transmission prevention-based control strategies, which could gain importance as H30-Rx and other extraintestinal pathogenic E. coli subclones become resistant to our best antibiotics.
Fabrication of a 35 GHz folded waveguide TWT circuit using rapid prototype techniques (2014)

Microfabrication techniques are commonly used to build circuits for millimeter-wave and THz vacuum electron devices. A cost effective solution is becoming available, at least for building prototype circuits intended for cold-testing. Rapid prototyping machines such as 3D printers have advanced to the point that their resolution is below the wavelength of many microwave circuits. This paper reviews the application of this quickly-advancing technology towards waveguide components of vacuum electron devices. The authors use a rapid prototype technique called Direct Metal Laser Sintering (DMLS) to “print” sample 35 GHz circuits in metal. Circuits in two different materials (aluminum and chromium cobalt) are printed and cold-tested. The test data shows good agreement with simulation.
Positive Allosteric Systems Designed on Dynamic Supramolecular Scaffolds:  Toward Switching and Amplification of Guest Affinity and Selectivity (2001)

Positive homotropic allosterism appears in important information transduction processes where chemical and physical signals are efficiently amplified. The phenomena are ubiquitous in nature, but the general methodology for the design of such allosteric systems is not yet established in an artificial system. This account reviews such artificial receptors that can bind guest ions and molecules in a positive allosteric manner and discusses what kinds of factors are indispensable as scaffolds in the design of this novel class of allosteric systems and what common factors are needed to realize the cooperativity. It has been shown that the scaffolds are mostly dynamic and are skillfully combined with the molecular recognition systems so that the subsequent guest binding can occur more favorably than the first guest binding. In addition, it has been suggested that positive homotropic allosterism can be utilized as a new strategy to attain high guest selectivity and guest affinity which cannot be attained by conventional 1:1-type guest binding.
Heterogeneity of plasma tissue factor pathway inhibitor. (1994)

Much of tissue factor pathway inhibitor (TFPI) in plasma is bound to lipoproteins. The major form of TFPI associated with low density lipoproteins (LDL) is 34 kDa, whereas that associated with high density lipoproteins (HDL) is 41 kDa and appears in part to represent a mixed disulphide complex between TFPI and apolipoprotein AII. The native and recombinant TFPI produced by mammalian cells in tissue culture and the TFPI released by heparin in vivo, however, are 34 kDa. Western blotting with antibodies raised against specific TFPI peptides and cation exchange chromatography under denaturing conditions of partially purified plasma TFPI suggest that only a fraction of TFPI circulating in plasma is in the form of the full length molecule, the remainder consisting of variably carboxyl-terminal truncated forms. Electrospray mass spectrometry of the isolated 34 kDa form of plasma TFPI, which predominantly circulates bound to LDL, confirms that it lacks a substantial portion of the carboxyl-terminus including at least a portion of the third Kunitz-type domain.
Pointer analysis for programs with structures and casting (1999)

Type casting allows a program to access an object as if it had a type different from its declared type. This complicates the design of a pointer-analysis algorithm that treats structure fields as separate objects; therefore, some previous pointer-analysis algorithms collapse a structure into a single variable. The disadvantage of this approach is that it can lead to very imprecise points-to information. Other algorithms treat each field as a separate object based on its offset and size. While this approach leads to more precise results, the results are not portable because the memory layout of structures is implementation dependent.This paper first describes the complications introduced by type casting, then presents a tunable pointer-analysis framework for handling structures in the presence of casting. Different instances of this framework produce algorithms with different levels of precision, portability, and efficiency. Experimental results from running our implementations of four instances of this framework show that (i) it is important to distinguish fields of structures in pointer analysis, but (ii) making conservative approximations when casting is involved usually does not cost much in terms of time, space, or the precision of the results.
Heterotopic Ossification of the Bilateral Elbows in Patients with Burns (2010)

25歳男性．灯油引火による上半身熱傷（両上肢III度熱傷，体幹・顔面II度）に対して，減張切開・創傷処置・植皮術を複数回施行された．受傷後5カ月で肘関節拘縮に対して当科紹介となる．可動域は右肘40°―50°，左肘45°―50°であり，単純XpおよびCTにおいて，上腕骨内側上顆後方から肘頭にかけての異所性骨化を認めた．併発した腎炎の治療を行い，症状が安定した受傷後16カ月で右肘関節，受傷後19カ月で左肘関節の関節授動術を行った．内側アプローチにて尺骨神経を剥離後，内側上顆を切除し，後斜走靱帯を切離した．外側アプローチも追加し，後方の骨化部分を切除した．術後肘装具を使用し可動域訓練を行い，両肘関節とも0―140°の可動域を獲得している．日常生活動作は自立し，患者満足度は高かった．
A context-aware framework supporting complex ubiquitous scenarios with Augmented Reality enabled (2010)

In this paper, we propose a context-aware system framework characteristic of two novel features. First, this framework is capable of modeling highly complex usage scenarios as a finite state machine while having transitions controlled by context information. And, this framework is designed with Augmented Reality capability as an integral component in its overall architecture to enable augmented interaction in context-aware applications. We believe this framework can be used ubiquitously in Context Aware Computing (CAC). As an example, a prototype has been developed for the application scenario of a manufacturing factory. We will also present the implementation details of this prototype.
Engineering an in vitro air-blood barrier by 3D bioprinting (2015)

Intensive efforts in recent years to develop and commercialize in vitro alternatives in the field of risk assessment have yielded new promising two- and three dimensional (3D) cell culture models. Nevertheless, a realistic 3D in vitro alveolar model is not available yet. Here we report on the biofabrication of the human air-blood tissue barrier analogue composed of an endothelial cell, basement membrane and epithelial cell layer by using a bioprinting technology. In contrary to the manual method, we demonstrate that this technique enables automatized and reproducible creation of thinner and more homogeneous cell layers, which is required for an optimal air-blood tissue barrier. This bioprinting platform will offer an excellent tool to engineer an advanced 3D lung model for high-throughput screening for safety assessment and drug efficacy testing.
Profiling of atmospheric turbulence strength and velocity using a generalised SCIDAR technique (1998)

The measurement of the strength and velocity of atmospheric optical turbulence using a generalised SCIDAR technique is outlined and demonstrated. This method allows the full turbulent profile to be characterised from the telescope pupil up to any desired altitude. A number of example profiles from various astronomical observing sites are presented.
Discovery of Novel Inhibitors of a Disintegrin and Metalloprotease 17 (ADAM17) Using Glycosylated and Non-glycosylated Substrates (2012)

A disintegrin and metalloprotease (ADAM) proteases are implicated in multiple diseases, but no drugs based on ADAM inhibition exist. Most of the ADAM inhibitors developed to date feature zinc-binding moieties that target the active site zinc, which leads to a lack of selectivity and off-target toxicity. We hypothesized that secondary binding site (exosite) inhibitors should provide a viable alternative to active site inhibitors. Potential exosites in ADAM structures have been reported, but no studies describing substrate features necessary for exosite interactions exist. Analysis of ADAM cognate substrates revealed that glycosylation is often present in the vicinity of the scissile bond. To study whether glycosylation plays a role in modulating ADAM activity, a tumor necrosis factor α (TNFα) substrate with and without a glycan moiety attached was synthesized and characterized. Glycosylation enhanced ADAM8 and -17 activities and decreased ADAM10 activity. Metalloprotease (MMP) activity was unaffected by TNFα substrate glycosylation. High throughput screening assays were developed using glycosylated and non-glycosylated substrate, and positional scanning was conducted. A novel chemotype of ADAM17-selective probes was discovered from the TPIMS library (Houghten, R. A., Pinilla, C., Giulianotti, M. A., Appel, J. R., Dooley, C. T., Nefzi, A., Ostresh, J. M., Yu, Y., Maggiora, G. M., Medina-Franco, J. L., Brunner, D., and Schneider, J. (2008) Strategies for the use of mixture-based synthetic combinatorial libraries. Scaffold ranking, direct testing in vivo, and enhanced deconvolution by computational methods. J. Comb. Chem. 10, 3-19; Pinilla, C., Appel, J. R., Borràs, E., and Houghten, R. A. (2003) Advances in the use of synthetic combinatorial chemistry. Mixture-based libraries. Nat. Med. 9, 118-122) that preferentially inhibited glycosylated substrate hydrolysis and spared ADAM10, MMP-8, and MMP-14. Kinetic studies revealed that ADAM17 inhibition occurred via a non-zinc-binding mechanism. Thus, modulation of proteolysis via glycosylation may be used for identifying novel, potentially exosite binding compounds. The newly described ADAM17 inhibitors represent research tools to investigate the role of ADAM17 in the progression of various diseases.
Tissue engineered biological augmentation for tendon healing: a systematic review (2010)

The optimization strategies discussed in this article are currently at an early stage of development. Although these emerging technologies may develop into substantial clinical treatment options, their full impact needs to be critically evaluated in a scientific fashion.