gebhart/patrepeval huggingface dataset:
Analysis of cartilage oligomeric matrix protein (COMP) in synovial fibroblasts and synovial fluids (1998)

We investigated the expression of cartilage oligomeric matrix protein (COMP) in normal and rheumatoid arthritis (RA) synovial fibroblasts. In situ hybridization (ISH) was conducted on synovial specimens from five RA patients applying specific probes for COMP or fibroblast collagen type I. ISH was combined with immunohistochemistry, applying antibodies to the macrophage marker CD68. Ribonuclease protection assay (RPA) and rapid amplification of 3'-cDNA ends (3'-RACE) were performed on total RNA from normal and RA synovial fibroblast cultures. Protein extracts from fibroblasts and culture supernatants were compared with synovial fluids and protein extracts from isolated chondrocytes by Western blot utilizing polyclonal and monoclonal antibodies (18-G3 mAb) to COMP. COMP mRNA was detected in fibroblasts of RA synovium by ISH, and in normal and RA synovial fibroblast cultures by RPA. 3'-RACE demonstrated sequence homology of chondrocyte and synovial fibroblast COMP along the coding sequence. COMP protein was detected in synovial fibroblasts and culture supernatants by immunoblot. Using polyclonal antibodies, the major portion of COMP from fibroblasts and culture supernatants was present as low-molecular-weight (LMW) bands, corresponding to those found in synovial fluids. These LMW COMP bands, however, were not detected in any of the cells or tissues tested using 18-G3 mAb. In protein extracts from chondrocytes and in COMP purified from cartilage, these LMW bands could not be detected. In conclusion, the data suggest that certain forms of COMP detected in synovial fluid are secreted from synovial fibroblasts and could be distinguished by specific mAbs from COMP secreted by chondrocytes.
Abstract #LB-5: Increased expression of phospho-Met (Tyr1349) is found in Non-Small Cell Lung Cancer metastases (2009)

Growth factor receptors are important in lung cancer growth and progression and are potential targets for non-small cell lung cancer (NSCLC) therapy. c-Met, which is overexpressed in many cancers, is a tyrosine kinase receptor activated by binding to its ligand, hepatocyte growth factor (HGF). Receptor phosphorylation occurs at multiple tyrosines and leads to multiple biological responses including cell proliferation, motility, angiogenesis and morphogenesis. Our objective was an immunohistochemical study of c-Met and phospho-Met (p-Met)(Y1349) expression in clinical specimens of NSCLC. While expression of p-Met (Y1003) and (Y1230/1234/1235) has been reported in lung cancer, to our knowledge this is the first study of p-Met (Y1349) expression in NSCLC or other solid human tumors. Archived FFPE tissues from 52 lung cancer patients that underwent surgery at St. Elizabeth Medical Center between 1986 and 2000 were stained. Expression of c-Met and p-Met was evaluated semi-quantitatively for stain intensity and % of tissue stained. Histoscores (intensity X area) were calculated for cytoplasmic and nuclear stain. Data were analyzed by the Least Squares Means with the Tukey-Kramer adjustment. Survival was determined by Kaplan-Meier statistics. 31/32 (97%) primary adenocarcinoma (AdCa) specimens and 29/31 (94%) primary squamous cell carcinoma (SCC) specimens had cytoplasmic/membranous c-Met histoscores (HSC) of 0.9 - >1.8; 24/35 (69%) AdCa and 23/35 (66%) SCC had p-Met HSC of 0.45 - >1.8. In 52 patients, average C-Met HSC > 1 was marginally related to reduced survival (p = 0.0970). Except in bronchial epithelium, c-Met and p-Met (Y1349) expression was less in normal lung components compared to NSCLC; the average c-Met HSC in primary NCLC was significantly higher than the average c-Met HSC of all lung components including bronchial epithelium (1.95 + 0.10 vs. 1.59 + 0.06, p = 0.0050). c-Met HSC was similar in 66 specimens of primary NSCLC and 24 lymph node metastases (1.96 + 0.08 vs. 1.79 + 0.17, p = 0.2487); however p-Met (Y1349) HSC was significantly higher in lymph node metastases than primary NSCLC (1.04 + 0.14 vs. 0.71 + 0.08, p = 0.0351) or the average of all normal lung components (0.75 + 0.05, p = 0.0455). Median p-Met HSC in 40 patients with primary tumors without metastases was 0.468 vs. 1.052 in lymph node metastases from 12 patients. The p-Met (Y1249) nuclear histoscore was also significantly higher in lymph node metastases (0.32 + 0.12 vs. 0.11 + 0.03, p = 0.0038). We conclude that c-Met is overexpressed in both AdCa and SCC and is marginally related to survival, and that c-Met phosphorylation at tyrosine 1349 is increased in NSCLC metastases. Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr LB-5.
Antitumour effects of a bispecific trivalent antibody in multicellular tumour spheroids. (2004)

These results demonstrate that bispecific antibodies are capable of activating immune effector cells, resulting in the elimination of three-dimensional structures of tumour cells. The therapeutic potential of these antibodies in the clinical setting merits further investigations.
Capillary electrophoresis of DNA for molecular diagnostics (1997)

A number of applications of capillary zone electrophoresis (CZE) in sieving liquid polymers (notably linear polyacrylamides and cellulose) for the analysis of polymerase chain reaction (PCR) products of clinically relevant, diagnostic DNA, are reviewed. The fields covered are: human genetics, quantitative gene dosage, microbiology and virology, forensic medicine and therapeutic DNA (notably, antisense nucleotides). Some unique, novel developments are highlighted, such as: (i) nonisocratic CZE, i.e., temperature-programmed CZE for detection of DNA point mutations; (ii) the synthesis of novel N-substituted acrylamides, offering extreme resistance to alkaline hydrolysis coupled to high hydrophilicity. In the field of denaturing gradient gel electrophoresis (DGGE), as routinely performed in gel slabs, a novel methodology is described in CZE: double-gradient DGGE. In this technique, two gradients are simultaneously applied along the migration direction: a chemical (or thermal) denaturing gradient, for partially unwinding homo- and hetero-duplexes of DNA, and a porosity gradient, for recompacting diffuse bands melting over a broader range of denaturing conditions. It is thus demonstrated that chemical gradients, in addition to temperature gradients, can be easily implemented even in a capillary format.
Analysis of Membrane Protein Complexes by Blue Native PAGE (2006)

Blue native polyacryamide gel electrophoresis is a special case of native electrophoresis for high resolution separation of enzymatically active protein complexes from tissue homogenates and cell fractions. The method is powerful between 10 and 10,000 kDa. Also membrane protein complexes are separated well after solubilization of complexes with mild neutral detergents. The separation principle relies on binding of Coomassie blue G250 which provides negative charges to the surface of the protein. During migration to the anode, protein complexes are separated according to molecular mass and/or size and high resolution is obtained by the decreasing pore size of a polyacrylamide gradient gel. The principles of 2-dimensional blue native sodium dodecyl sulfate polyacrylamide gel electrophoresis are presented here together with a practical step-by-step guide to performing the method in the laboratory.
Severity of <i>Clostridium difficile</i>–Associated Disease (CDAD) in Allogeneic Stem Cell Transplant Recipients: Evaluation of a CDAD Severity Grading System (2007)

The purpose of this study was to develop and test a Clostridium difficile-associated disease (CDAD) grading system based on presenting symptoms in allogeneic stem cell transplant recipients. Patients with severe CDAD had significantly shorter median survival times and more adverse outcomes than patients with mild or moderate CDAD.
Detection and quantification of free sulfhydryls in monoclonal antibodies using maleimide labeling and mass spectrometry (2019)

The detection of free sulfhydryls in proteins can reveal incomplete disulfide bond formation, indicate cysteine residues available for conjugation, and offer insights into protein stability and structure. Traditional spectroscopic methods of free sulfhydryl detection, such as Ellman's reagent, generally require a relatively large amount of sample, preventing their use for the analysis of biotherapeutics early in the development cycle. These spectroscopic methods also cannot accurately determine the location of the free sulfhydryl, further limiting their utility. Mass spectrometry was used to detect free sulfhydryl residues in intact proteins after labeling with Maleimide-PEG<sub>2</sub>-Biotin. As little as 2% cysteine residues with free sulfhydryls (0.02 mol SH per mol protein) could be detected by this method. Following reduction, the free sulfhydryl abundance on antibody heavy and light chains could be measured. To determine free sulfhydryl location at peptide-level resolution, free sulfhydryls and cysteines involved in disulfide bonds were differentially labeled with N-ethylmaleimide and d<sub>5</sub>-N-ethylmaleimide, respectively. Following enzymatic digestion and nanoLC-MS, the abundance of free sulfhydryls at individual cysteine residues was quantified down to 2%. The method was optimized to avoid non-specific labeling, disulfide bond scrambling, and maleimide exchange and hydrolysis. This new workflow for free sulfhydryl analysis was used to measure the abundance and location of free sulfhydryls in 3 commercially available monoclonal antibody standards (NIST Monoclonal Antibody Reference Material (NIST), SILu™Lite SigmaMAb Universal Antibody Standard (Sigma-Aldrich) and Intact mAb Mass Check Standard (Waters)) and 1 small protein standard (β-Lactoglobulin A).
Comparison of the Long-term Results of Roux-en-Y Gastric Bypass and Sleeve Gastrectomy for Morbid Obesity (2014)

RYGB was more effective than SG in the resolution of obesity-related comorbidities, SG was a safer procedure with a reduced rate of complications and reoperation.
Regeneration of a Lytic Central Vacuole and of Neutral Peripheral Vacuoles Can Be Visualized by Green Fluorescent Proteins Targeted to Either Type of Vacuoles (2001)

Protein trafficking to two different types of vacuoles was investigated in tobacco (Nicotiana tabacum cv SR1) mesophyll protoplasts using two different vacuolar green fluorescent proteins (GFPs). One GFP is targeted to a pH-neutral vacuole by the C-terminal vacuolar sorting determinant of tobacco chitinase A, whereas the other GFP is targeted to an acidic lytic vacuole by the N-terminal propeptide of barley aleurain, which contains a sequence-specific vacuolar sorting determinant. The trafficking and final accumulation in the central vacuole (CV) or in smaller peripheral vacuoles differed for the two reporter proteins, depending on the cell type. Within 2 d, evacuolated (mini-) protoplasts regenerate a large CV. Expression of the two vacuolar GFPs in miniprotoplasts indicated that the newly formed CV was a lytic vacuole, whereas neutral vacuoles always remained peripheral. Only later, once the regeneration of the CV was completed, the content of peripheral storage vacuoles could be seen to appear in the CV of a third of the cells, apparently by heterotypic fusion.
Plasma concentrations of individual tea catechins after a single oral dose in humans (2001)

1. Ten healthy volunteers ingested 1.5 mmole epicatechin gallate (ECg), epigallocatechin (EGC) or epigallocatechin gallate (EGCg) in a randomized crossover design. After deconjugation, catechins in plasma and 24-h urine samples were determined by high-performance liquid chromatography (HPLC). Antioxidant activity was measured in plasma by determining ferric reducing activity (FRAP). 2. The catechin levels in plasma after ingestion were significantly different: EGC rose quickly with a short elimination half-life (t1/2 elim = 1.7 h), ECg was intermediate in rise but slowest in decline (t1/2 elim = 6.9h), EGCg was slowest in rise but intermediate in decline (t1/2 elim = 3.9h). At 24h, EGC and EGCg had returned to base levels, but ECg was still elevated. Peak maximum varied between 1.3 (EGCg) and 5.0 micromol l(-1) (EGC). 3. Very limited interconversion (ECg-->epicatechin, EGCg-->EGC) occurred indicating that degallation is not required for uptake. 4. Up to 13.6% of the ingested EGC (partly methylated) was excreted in the urine, but ECg or EGCg were not detected. 5. EGC and ECg produced an increase in antioxidant activity in plasma, but with EGCg, no statistically significant effect was found. 6. The pattern of uric acid in plasma showed a clear resemblance with that of FRAP and linear regression analysis indicated a very significant relationship (R2 = 0.88, p < 0.0001). 7. It is concluded that tea catechins differ significantly in their pharmacokinetic behaviour.
A behavioral SPICE compatible model of a self-oscillating converter (2005)

A SPICE compatible model for simulating self-oscillating dc-dc converters that are based on magnetic saturation is developed and tested. The simulation method applies a non-linear inductor model that reflects a linear inductor in a non-linear manner. The model was tested on a flyback type self-oscillating converter by simulation and was verified experimentally.
The Epstein-Barr Virus Immediate-Early Protein BZLF1 Regulates p53 Function through Multiple Mechanisms (2002)

The Epstein-Barr virus (EBV) immediate-early protein BZLF1 is a transcriptional activator that mediates the switch between the latent and the lytic forms of EBV infection. It was previously reported that BZLF1 inhibits p53 transcriptional function in reporter gene assays. Here we further examined the effects of BZLF1 on p53 function by using a BZLF1-expressing adenovirus vector (AdBZLF1). Infection of cells with the AdBZLF1 vector increased the level of cellular p53 but prevented the induction of p53-dependent cellular target genes, such as p21 and MDM2. BZLF1-expressing cells had increased p53-specific DNA binding activity in electrophoretic mobility shift assays, increased p53 phosphorylation at multiple residues (including serines 6, 9, 15, 33, 46, 315, and 392), and increased acetylation at lysine 320 and lysine 382. Thus, the inhibitory effects of BZLF1 on p53 transcriptional function cannot be explained by its effects on p53 phosphorylation, acetylation, or DNA binding activity. BZLF1 substantially reduced the level of cellular TATA binding protein (TBP) in both normal human fibroblasts and A549 cells, and the inhibitory effects of BZLF1 on p53 transcriptional function could be partially rescued by the overexpression of TBP. Thus, BZLF1 has numerous effects on p53 posttranslational modification but may inhibit p53 transcriptional function in part through an indirect mechanism involving the suppression of TBP expression.
A novel technique for high speed uplink data transmission in 3G systems (2003)

In 3G systems, the achievable peak data rates on the uplink are much smaller than the downlink data rates due to noise rise constraints resulting from the nonorthogonal nature of the CDMA uplink. In this paper, we present a new time-orthogonal approach called high-speed data burst transmission mode. The concept is based on slot-synchronized slot-orthogonal transmissions whereby high-speed data transmissions take place in slots orthogonal to the slots used for low data rate transmission and physical layer control signaling. Using this approach very high data rates are achieved during data burst transmission because the noise rise constraint is relaxed due to the orthogonal nature of the transmissions. The simulation results show that the uplink capacity of the proposed scheme is comparable to the downlink capacity thus making the system symmetrical.
Effects of subchronic antidepressant drug treatment on central serotonergic mechanisms in the male rat. (1982)

These studies have examined the effects of a 2 week oral treatment with zimelidine, desipramine and imipramine in clinically relevant doses on 5-HT-1 and 5-HT-2 receptors, on 5-HT synthesis, on 5-HT dependent behaviours and on prolactin secretion in the male rat. 1. Zimelidine, desipramine and imipramine produced the same type of changes in 5-HT-1 and 5-HT-2 binding sites of the cerebral cortex. 2. A marked and selective reduction of 5-HT synthesis could be observed following long-term zimelidine treatment. 3. Long-term zimelidine and desipramine treatment reduced head twitch behaviour in mice induced by 5-HTP and 5-MeODMT. 4. Long-term treatment with zimelidine produced a reduction of basal prolactin secretion. These results suggest that long-term treatment with zimelidine desipramine and imipramine may reduce 5-HT neurotransmission at least in some 5-HT synapses in the brain which may represent part of the basis for their therapeutic activity. The indication of large numbers of low affinity binding sites (10-40 nM) for 3H-5-HT sites may lead to less steep dose effect curves and to a staprolactin secretion while long-term treatment with zimelidine results in significant reductions of prolactin secretion (11). These results support the view of the existence of a reduction of 5-HT neurotransmission at least within the hypothalamus following long-term treatment with zimelidine. Also other behavioural findings suggest that adaptive changes occur in 5-HT synapses involved in regulating the "serotonin syndrome" after long-term antidepressant treatment. Thus, the 5-HT syndrome is no longer enhanced by 5-HT uptake blocking agents. The ability of fluoxetine to enhance haloperidol-induced increases of dopamine metabolites in the striatum also disappears following chronic fluoxetine treatment (6,34).
Electrocardiogram Recognization Based on Variational AutoEncoder (2018)

Subtle distortions on electrocardiogram (ECG) can help doctors to diagnose some serious larvaceous heart sickness on their patients. However, it is difficult to find them manually because of disturbing factors such as baseline wander and high-frequency noise. In this chapter, we propose a method based on variational autoencoder to distinguish these distortions automatically and efficiently. We test our method on three ECG datasets from Physionet by adding some tiny artificial distortions. Comparing with other approaches adopting autoencoders [e.g., contractive autoencoder, denoising autoencoder (DAE)], the results of our experiment show that our method improves the performance of publically available on ECG analysis on the distortions.
A round turbulent jet produced by an oscillating diaphragm (1996)

A round turbulent water jet produced normal to, and at the center of a submerged, resonantly driven diaphragm is investigated experimentally. The jet which is formed without mass injection and is comprised entirely of radially entrained fluid, is present only when the excitation amplitude exceeds a given threshold. Above this excitation level, a small cluster of cavitation bubbles appears near the center of the diaphragm. The bubbles grow, apparently collapse, and then disappear during each oscillation cycle. It is conjectured that the jet is synthesized by time-periodic coalescence of vortex rings that are produced by secondary flow around the bubbles or by the collapse of the bubbles. It is remarkable that even though the jet results from a strong time-periodic excitation and its time-periodic features are detected throughout the present range of measurements, the time-averaged jet structure is similar to that of a conventional turbulent round jet in that the increase in its width and in the inverse of its centerline velocity are both linear functions of the distance from the actuator. In contrast to conventional jets, the present synthetic jets can be manipulated on relatively short time scales that are comparable to the excitation period.
SHORT NOTICES (1985)

SHORT NOTICES Get access Persecution and Toleration. Edited by W. J. SHEILS. Pp. xvi³490. (Studies in Church History 21.) Oxford: Basil Blackwell, for the Ecclesiastical History Society, 1984. £25. H. CHADWICK H. CHADWICK Search for other works by this author on: Oxford Academic Google Scholar The Journal of Theological Studies, Volume 36, Issue 2, October 1985, Pages 565-b–565, https://doi.org/10.1093/jts/36.2.565-b Published: 01 October 1985
A learning approach to personalized information filtering (1994)

A personalized information filtering system must specialize to current interests of the user and adapt as they change over time. It must also explore newer domains for potentially interesting information. A learning approach to building personalized information filtering systems is proposed. The system is designed as a collection of information filtering interface agents. Interface Agents are intelligent and autonomous computer programs which learn users&apos; preferences and act on their behalf --- electronic personal assistants that automate tasks for the user. This thesis presents the basic framework for personalized information filtering agents, and describes an implementation, &quot;Newt&quot;, built using the framework. Newt uses a keyword based filtering algorithm. The learning mechanisms used are relevance feedback and the genetic algorithm. The user interface is friendly and accessible to both naive as well as power users. Experimental results indicate that Newt can be personalized to serve ...
Photoinduced Switching from Living Radical Polymerization to a Radical Coupling Reaction Mediated by Organotellurium Compounds (2012)

An efficient and simple method for the synthesis of symmetric macromolecules by photoinduced switching from radical polymerization to a radical coupling reaction is reported. Structurally well-defined telechelic polyisoprenes and ABA-triblock copolymers were prepared by successive organotellurium-mediated living radical polymerization (TERP) under thermal conditions, followed by a polymer-end radical coupling reaction under photoirradiation.
34.1: High‐Luminance and High‐Efficacy Electric‐Field‐Coupled Discharge Lamp for 18‐in. Diagonal LCD Backlighting (1999)

Abstract A flat lamp is developed for an 18‐in.‐diagonal LCD backlighting, using 8 electric‐field‐coupled discharge tubes. All the tubes can be driven with a single inverter circuit. Luminance, efficacy, and input power are 3500cd/m 2 , 34 lm/W, and 35W when the lamp is driven at 5.24MHz and 790V.
Anthocyanin leaf markings are regulated by a family of <i>R2R3‐MYB</i> genes in the genus <i><scp>T</scp>rifolium</i> (2014)

Anthocyanin pigments accumulate to form spatially restricted patterns in plants, particularly in flowers, but also occur in vegetative tissues. Spatially restricted anthocyanin leaf markings are poorly characterised in plants, but are common in forage legumes. We hypothesised that the molecular basis for anthocyanin leaf markings in Trifolium spp. is due to the activity of a family of R2R3-MYB genes. R2R3-MYB genes were identified that are associated with the two classic pigmentation loci in T. repens. The R locus patterns 'red leaf', 'red midrib' and 'red fleck' are conditioned by a single MYB gene, RED LEAF. The 'diffuse red leaf' trait is regulated by the RED LEAF DIFFUSE MYB gene. The V locus was identified through mapping two V-linked traits, 'V-broken yellow' (Vby) and 'red leaflet' (Vrl). Two highly similar R2R3-MYB genes, RED V-a and RED V-b, mapped to the V locus and co-segregated with the RED V pigmentation pattern. Functional characterisation of RED LEAF and RED V was performed, confirming their function as anthocyanin regulators and identifying a C-terminal region necessary for transactivation. The mechanisms responsible for generating anthocyanin leaf markings in T. repens provide a valuable system to compare with mechanisms that regulate complex floral pigmentation.
Utvecklingsmiljö för Produktmodelleringssystem (1995)

Komplexiteten i hogteknologiska produkter som kraftverk, flygplan, etc. okar standigt. Produktutvecklingstiderna maste minskas samtidigt som design och kvalitet ska forbattras pa ett kostnadseffekt ...