gebhart/patrepeval huggingface dataset:
Dietary fat and colon cancer: modulation of cyclooxygenase-2 by types and amount of dietary fat during the postinitiation stage of colon carcinogenesis. (1997)

Several epidemiological and experimental studies have indicated a strong relationship between different types of dietary fats and risk of colon cancer. However, the modulating effects of these nutritional factors at the molecular level are not fully elucidated. It has been documented that the modulation of tumorigenesis is associated with changes in the levels of prostaglandins production. To provide an understanding of the mechanism(s) of dietary fat-induced modulation of colon tumor development, we have analyzed the expression of cyclooxygenase (COX), an enzyme that catalyzes the metabolism of omega-3 and omega-6 polyunsaturated fatty acids in a critical step in prostaglandin biosynthesis. Male F344 rats were fed the semipurified AIN-76A diet containing low-fat corn oil and were s.c. injected azoxymethane (AOM) dissolved in normal saline at a dose rate of 15 mg/kg of body weight, once weekly, for 2 weeks. Vehicle controls received s.c. equal volumes of normal saline. One day after the second AOM or saline injection, rats intended to receive experimental diets were provided high-fat corn oil (HFCO) or high-fat fish oil (HFFO) mixed in semipurified AIN-76A diet, and the remaining rats continued to receive the low-fat corn oil diet. Animals were sacrificed 1, 12, or 36 weeks after the last AOM or saline injection, and their colonic mucosa, as well as the grossly visible colon tumors from rats sacrificed 36 weeks after the last AOM injection, were analyzed for the expression levels of COX-1 and COX-2. The results indicate that AOM induced increasingly high levels of COX-2 expression with advancing stages of colon tumorigenesis. HFCO further enhanced the AOM-induced COX-2 expression. In contrast, HFFO ingestion resulted in a significant decrease in COX-2 expression both in the colonic mucosa and in tumors. These results correlate with increased incidence and multiplicity of grossly visible colon tumors in AOM-treated animals that were fed the HFCO diet versus decreased tumor incidence and lower tumor multiplicity in animals that were fed the HFFO diet. No significant differences were observed in expression levels of COX-1. The data suggest that HFCO may promote colon tumorigenesis by up-regulating the COX-2 expression, whereas HFFO may exert its antitumor effect by inhibiting the COX-2 expression.
High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies (2015)

Mesenchymal stem cells (MSCs) derived from bone marrow are a powerful cellular resource and have been used in numerous studies as potential candidates to develop strategies for treating a variety of diseases. The purpose of this study was to develop and characterize MSCs as cellular vehicles engineered for delivery of therapeutic factors as part of a neuroprotective strategy for rescuing the damaged or diseased nervous system. In this study we used mouse MSCs that were genetically modified using lentiviral vectors, which encoded brain-derived neurotrophic factor (BDNF) or glial cell-derived neurotrophic factor (GDNF), together with green fluorescent protein (GFP). Before proceeding with in vivo transplant studies it was important to characterize the engineered cells to determine whether or not the genetic modification altered aspects of normal cell behavior. Different culture substrates were examined for their ability to support cell adhesion, proliferation, survival, and cell migration of the four subpopulations of engineered MSCs. High content screening (HCS) was conducted and image analysis performed. Substrates examined included: poly-L-lysine, fibronectin, collagen type I, laminin, entactin-collagen IV-laminin (ECL). Ki67 immunolabeling was used to investigate cell proliferation and Propidium Iodide staining was used to investigate cell viability. Time-lapse imaging was conducted using a transmitted light/environmental chamber system on the high content screening system. Our results demonstrated that the different subpopulations of the genetically modified MSCs displayed similar behaviors that were in general comparable to that of the original, non-modified MSCs. The influence of different culture substrates on cell growth and cell migration was not dramatically different between groups comparing the different MSC subtypes, as well as culture substrates. This study provides an experimental strategy to rapidly characterize engineered stem cells and their behaviors before their application in long-term in vivo transplant studies for nervous system rescue and repair.
Hepatic expression of <i>miR‐122, miR‐126, miR‐136</i> and <i>miR‐181a</i> and their correlation to histopathological and clinical characteristics of patients with hepatitis C (2014)

It has been shown for hepatitis C virus (HCV) infection that host miRNAs contribute to the replication of the viral RNA genome. However, the clinical impact of these and many other cellular miRNAs on HCV in humans is still largely unclear. We therefore analysed the expression of miR-122, miR-126, miR-181a and miR-136 in HCV-infected patients. The study included liver biopsies of 65 patients infected with HCV of different genotypes (gt 1, gt 1a, gt 1b, gt 3 and gt 4) and nine noninfected individuals. Expression analysis of miRNAs was performed by qPCR, and they were analysed for differences between patient gender and age, genotypes, stage of fibrosis, grade of inflammation, serum level of liver enzymes, serum viral load, the presence of steatosis and mode of transmission. Different target prediction algorithms were used to search for targets of analyzed miRNAs. Statistical analysis revealed significant up-regulation of miR-136 and down-regulation of miR-126 and miR-181a in patients infected with HCV of different genotypes compared with noninfected individuals. The same expression pattern was observed in different stages and grades of liver disease. miR-122 was up-regulated in women relative to men and associated to portal inflammation, miR-122 and miR-126 correlated with serum HCV load and miR-136 and miR-122 correlated with the presence of steatosis. miR-126 and miR-136 were differentially expressed between different modes of HCV transmission. There were approximately 2000 different targets predicted for all four miRNAs and each of the analyzed miRNAs could be involved in more than a 100 different biochemical pathways. miR-122, miR-126, miR-136 and miR-181a have been shown to be involved in HCV infection with different genotypes. Their expression has been associated with the gender, stage and grade of liver disease, mode of transmission, serum HCV load and the presence of steatosis. Numerous target genes and biochemical pathways are predicted for each of the analyzed miRNAs. All these results suggest their role in HCV-infected liver disease.
PC based data communications system simulation (1990)

Computer simulation COMSIM using a low-cost desktop computer has been developed to aid the design, evaluate system performance, and perform tradeoffs of system parameters to achieve optimal communications system design. A modular simulation program structure is selected to develop generalized mathematical models and integrated software for modules such as the coder/decoder, modulator/demodulator, filters, and transmission channel. The program provides flexibility for modifying modules for each different system design. The simulation can be used to study circuit error sources in practical communications system design, such as circuit nonlinearities, filter distortions, interfering signals generated inside or outside the system, and propagation effects of the transmission path.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Model-based deformable surface finding for medical images (1996)

Describes a new global shape parameterization for smoothly deformable three-dimensional (3-D) objects, such as those found in biomedical images, whose diversity and irregularity make them difficult to represent in terms of fixed features or parts. This representation is used for geometric surface matching to 3-D medical image data, such as from magnetic resonance imaging (MRI). The parameterization decomposes the surface into sinusoidal basis functions. Four types of surfaces are modeled: tori, open surfaces, closed surfaces and tubes. This parameterization allows a wide variety of smooth surfaces to be described with a small number of parameters. Extrinsic model-based information is incorporated by introducing prior probabilities on the parameters. Surface finding is formulated as an optimization problem. Results of the method applied to synthetic images and 3-D medical images of the heart and brain are presented.
<i>In Vivo</i> Adenovirus-Mediated Gene Transfer of the <i>Escherichia coli</i> Cytosine Deaminase Gene to Human Colon Carcinoma-Derived Tumors Induces Chemosensitivity to 5-Fluorocytosine (1995)

To evaluate the concept that in vivo transfer of the Escherichia coli cytosine deaminase gene will confer sensitivity of a solid tumor to the prodrug 5-fluorocytosine (5FC), we constructed an adenovirus vector (AdCMV.CD) carrying the cytosine deaminase gene driven by the cytomegalovirus (CMV) promoter, infected HT29 colon carcinoma cells in vitro and in vivo, and evaluated cell growth over time. AdCMV.CD produced a functional cytosine deaminase protein in HT29 cells in vitro as evidenced by the ability of lysates from the infected cells to convert [3H]5FC to its active metabolite 5-fluorouracil (5FU). The AdCMV.CD vector effectively suppressed HT29 cell growth in vitro in the presence of 5FC in a dose-dependent manner. Infection with AdCMV.CD, when as few as 10% of cells expressed the cytosine deaminase gene, was associated with a bystander effect when combined with 5FC in cell mixing studies. Further, this bystander effect was not dependent on cell-to-cell contact as demonstrated by suppression of [3H]thymidine incorporation in HT29 cells when supernatant from AdCMV.CD-infected cells treated with 5FC was transferred cells. Consistent with these in vitro observations, when AdCMV.CD was directly injected into established subcutaneous HT29 tumors in nude mice receiving 5FC, there was a four-fold reduction in tumor size at day 15 compared to controls, and a five-fold reduction at day 28. These observations suggest that adenovirus-mediated gene transfer of the E. coli cytosine deaminase gene and concomitant administration of 5FC may have potential as a strategy for local control of the growth of tumor cells susceptible to 5FU.
Active Streamer Positioning - in Action (2002)

P087 ACTIVE STREAMER POSITIONING – IN ACTION Summary 1 At the 62nd meeting of the European Association of Geoscientists and Engineers in Glasgow a new system for marine seismic cable steering and control was described (Bittleston et al. 2000). In this paper recent operational experience with this system is reported together with an assessment of the results obtained. Introduction A. CURTIS 1 L. COMBEE 2 and W. OLAFSEN 2 1 WesternGeco Schlumberger House Buckingham Gate Gatwick Airport West Sussex RH6 0NZ UK 2 WesternGeco Oslo Technology Center In recent years the driver for towed marine acquisition systems has been cost-effective
Polyphase modulation as a solution of certain filtration problems in telecommunication (1950)

An important class of filtration problems in telecommunication is associated with frequency changing; it includes the generation and demodulation of single-sideband carrier channels and the elimination of image-frequency interference in heterodyne demodulators, such as the superheterodyne radio receiver or the conventional wave analyser. Filters for these applications are often difficult to design or realize, or may be inconvenient on account of variable tuning, etc.It has been realized recently that polyphase modulation can be used as part of the frequency-changing process with great advantage. It can eliminate the need for difficult or inconvenient filters, although other design problems are introduced which may some times be as difficult to solve. But there is no doubt that the idea of polyphase modulation is of great importance in telecommunication theory and practice. The basis of the advantages given by polyphase working is that polyphase signals possess an identifying property additional to that of frequency, namely sequence. By using circuits which distinguish between signals of the same frequency but opposite sequence, it is possible, without any preliminary filtration, to separate signals which lie in the same frequency band after modulation, and which, therefore, could be separated by normal means only by filters before the modulation stage.The first Section of the paper outlines the main filtration problems which can be tackled by polyphase methods, and then the necessary polyphase theory is given. This is followed by a discussion of circuit design for polyphase modulation and sequence discrimination.The list of references shows that there have been many publications covering some of the separate applications of this work, but the paper is believed to present for the first time a comprehensive theory of polyphase modulation embracing all the known applications.
Expressive facial caricature drawing (1999)

The purpose of this paper is to add facial expressions to an expressionless line drawing facial caricature which is drawn using linguistic terms. Questionnaire about the comparison of expressive facial caricatures and expressionless ones is performed in order to obtain essential parameter values and their essential combinations. Expressive facial caricatures are drawn using the essential combinations and the transformation from parameter values in an expressionless face to essential parameter values in an expressive one. Finally some examples of expressive facial caricature drawn by the presented method are shown.
Circulating T Cells of Patients with Active Psoriasis Respond to Streptococcal M‐Peptides Sharing Sequences with Human Epidermal Keratins (1997)

Psoriasis is a T-cell mediated inflammatory skin disease which has been associated with group A, beta-haemolytic streptococcal infections. Four 20 a.a. long M6-peptides sharing 5-6 a.a. sequences with human epidermal keratins were identified. To investigate the role of potentially cross-reactive T cells in the pathogenesis of psoriasis, interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) responses of circulating T cells to these peptides were analysed by ELISPOT and RT-PCR in 14 psoriatic patients, 12 healthy individuals and six patients with atopic dermatitis (AD). Untreated psoriatic patients' responses were significantly higher to these peptides than healthy and AD controls, while responses to a control M6-peptide, not sharing sequences with keratin, were negligible in all groups. No difference was found in response to streptokinase/streptodornase (SK/SD). M6-protein and peptides exclusively elicited IFN-gamma production, with little IL-4 production, even in AD patients. Interferon-gamma responses to all the M6-peptides were abolished after successful treatment of psoriatic patients, but responses to SK/SD were unaffected. The results indicate that active psoriasis is associated with Th1-like cells responding to streptococcal M6-peptides sharing sequences with human epidermal keratin. This is consistent with the hypothesis that psoriasis may be induced and exacerbated in susceptible individuals by M-protein specific Th1-like cells that cross-react with human epidermal keratin.
Characteristic Enhancement of Solution-Processed In–Ga–Zn Oxide Thin-Film Transistors by Laser Annealing (2010)

We applied laser annealing on indium-gallium-zinc oxide (IGZO) thin-film transistors (TFTs) to improve their electrical characteristics. The IGZO nanoparticles were prepared from a water solution, and the films were fabricated by the spin-coating method and postbaked at a low postbake temperature (95°C). The crystallinity and compositions of the as-deposited film and the laser-annealed films were analyzed by X-ray diffraction. The film morphology and the electrical characteristics of TFTs at various irradiation dosages are also presented. Results show that the field-effect mobility can be improved to 7.65 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /V·s.
Qualitative and quantitative analysis of human cytotoxic T-lymphocyte responses to HIV-1 proteins (1992)

Taken together, these results are encouraging for vaccine purposes, since anti-HIV-1 CTL stimulation is thought to be a requirement for such a vaccine.
The Benefits of Building Scale for Population Health (2016)

Alan Zuckerman Alan is one of the nation’s leading healthcare strategists, and has directed development of advanced strategic and business plans for many of the nation’s top academic medical centers and health systems in his 40 years of management consulting. He serves as strategic advisor to many long-term clients. He is recognized for his ability to bring unique strategic solutions to providers in highly dynamic markets and for his expertise in developing consensus among board members, medical staff, and management. Mr. Zuckerman’s work focuses primarily on organizational strategy projects, and mergers and affiliations. He is a frequent speaker at national healthcare conferences, and teaches seminars for the American College of Healthcare Executives (ACHE). Mr. Zuckerman has received the ACHE Service award in honor of his commitment to the healthcare management profession, and has also been awarded ACHE’s Senior‐Level Healthcare Executive Regent’s Award. John Harris John combines consulting expertise with a hands-on management background. His more than 25 years of healthcare experience includes founding a healthcare business, running facilities and consulting to hospitals and health systems, ACOs, Clinically Integrated Networks (CINs), PHOs, IPAs, and health plans. Mr. Harris’s experience includes founding Advance Choice, Inc., the leading national service providing hospitals with emergency access to patients’ advance medical directives. He has had senior operational responsibility in healthcare facilities. He has also been a health policy fellow for the US Senate’s Finance Committee. Presentation: 51:49 PowerPoint slides at bottom of page.
Goal accomplishment tracking for automatic supervision of plan execution (2013)

It is common practice to break down plans into a series of goals or sub-goals in order to facili-tate plan execution, thereby only burdening the individual agents responsible for their execu-tion with small, easily achievable objectives at any one time, or providing a simple way of shar-ing these objectives amongst a group of these agents. Ensuring that plans are executed cor-rectly is an essential part of any team manage-ment. To allow proper tracking of an agent’s progress through a pre-planned set of goals, it is imperative to keep track of which of these goals have already been accomplished. This centralised approach is essential when the agent is part of a team of humans and/or robots, and goal accomplishment is not always being tracked at a low level. This paper presents a framework for an automated supervision sys-tem to keep track of changes in world states so as to chart progress through a pre-planned set of goals. An implementation of this framework on a mobile service robot is presented, and ap-plied in an experiment which demonstrates its feasibility. 1
HIV-1 gp120 and anti-gp120 induce reversible unresponsiveness in peripheral CD4 T lymphocytes. (1994)

Human immunodeficiency virus type 1 (HIV-1) gp-120 potentially plays an important role in inducing functional suppression and depletion of CD4 lymphocytes following infection with HIV. In order to further understand the mechanisms involved in HIV-induced immunosuppression, we have studied the effects of recombinant HIV-1 gp120/SF2 and anti-gp120/SF2 antibodies on T cell receptor (TCR)-mediated proliferation of peripheral blood mononuclear cells (PBMCs) and isolated lymphocyte subsets from HIV-seronegative donors. In a dose-dependent manner, gp120 significantly reduces the proliferative responses of unfractionated PBMCs and highly enriched CD4 T lymphocytes when they are polyclonally stimulated through the TCR using WT31 (anti-alpha beta Ti chains) and anti-Leu 4 (anti-CD3 epsilon) in the presence of autologous accessory cells. The addition of divalent anti-gp120/SF2 to lymphocytes previously incubated with gp120 further reduces the proliferation to the levels seen after pretreating cells with divalent anti-CD4 (anti-Leu 3a). CD8 T lymphocytes, on the other hand, show no change in TCR-mediated proliferation following preincubation with either anti-CD4 or gp120/anti-gp120. We find no evidence for significant cell death by apoptosis using methods of DNA analysis or flow cytometry and DNA-specific dyes to account for the loss of CD4 lymphocyte proliferation. Interleukin-2 restores the proliferation suppressed by gp120/anti-gp120 suggesting the induction of reversible functional anergy.
General Synthesis of Quarter-Wave Impedance Transformers (1957)

This paper presents the general synthesis of a radio frequency impedance transformer of n quarter-wave steps, given an "insertion loss function" of permissible form. This procedure parallels that of Darlington for lumped constant filters by providing the connection between Collin's canonical form for the insertion loss function and Richards' demonstration that a reactance function may always be realized as a cascade of equal length impedance transformers terminated in either a short or open circuit. In particular, it is shown that insertion loss functions of the form selected by Collin are always realizable with positive characteristic impedances, and that the synthesis procedure, for maximally flat and Tchebycheff performance, involves the solution, at most, of quadratic equations. In addition, this procedure permits the proof of Collin's conjecture that, for his insertion loss function, the resulting reflection coefficients are symmetrical. Finally, closed expressions are given for the coefficients of the input impedance of a given n section transformer in terms of the n characteristic impedances and vice versa.
In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF -7 cell lines study of Marine Yeast (2015)

Marine yeasts isolated from coastal mangrove ecosystem namely Candida albicans, Kuraishia capsulate and Sacchromyces cerevisae were screened for the cytotoxicity against a non-transformed Vero (African green monkey kidney normal cell line) and two cancer cell lines human breast carcinoma cells (HepG2), human breast carcinoma cells (MCF -7) in different concentrations (1000 to 1.953 g/ml). Lower doses enhanced the viability of the cultured cells, MTT assay .higher doses decreased viability of the cells by 50% or more. MTT assay was used to measure the cell proliferation and survival. Amongst three yeast strains, Sacchromyces cerevisae showed more than 80% cell viability in Vero cell lines and were studied for further cytotoxicity against HepG2, MCF -7 cell lines respectively.