gebhart/patrepeval huggingface dataset:
Assembly of the mammalian muscle acetylcholine receptor in transfected COS cells. (1991)

We have investigated the mechanisms of assembly and transport to the cell surface of the mouse muscle nicotinic acetylcholine receptor (AChR) in transiently transfected COS cells. In cells transfected with all four subunit cDNAs, AChR was expressed on the surface with properties resembling those seen in mouse muscle cells (Gu, Y., A. F. Franco, Jr., P.D. Gardner, J. B. Lansman, J. R. Forsayeth, and Z. W. Hall. 1990. Neuron. 5:147-157). When incomplete combinations of AChR subunits were expressed, surface binding of 125I-alpha-bungarotoxin was not detected except in the case of alpha beta gamma which expressed less than 15% of that seen with all four subunits. Immunoprecipitation and sucrose gradient sedimentation experiments showed that in cells expressing pairs of subunits, alpha delta and alpha gamma heterodimers were formed, but alpha beta was not. When three subunits were expressed, alpha delta beta and alpha gamma beta complexes were formed. Variation of the ratios of the four subunit cDNAs used in the transfection mixture showed that surface AChR expression was decreased by high concentrations of delta or gamma cDNAs in a mutually competitive manner. High expression of delta or gamma subunits also each inhibited formation of a heterodimer with alpha and the other subunit. These results are consistent with a defined pathway for AChR assembly in which alpha delta and alpha gamma heterodimers are formed first, followed by association with the beta subunit and with each other to form the complete AChR.
6-Substituted 1,3,4,5-tetrahydrobenz[cd]indol-4-amines: potent serotonin agonists (1988)

A series of 6-substituted tricyclic ergoline partial structures has been synthesized and found to possess very strong serotonin agonist activity. A methoxy group at the 6-position greatly enhances activity, but at the expense of compound stability. Substituting the 6-position with protophyllic groups that are also electron-withdrawing in character enhances both activity and stability.
Ordered and Reversible Hydrogenation of Silicene (2015)

The hydrogenation of monatomic silicene sheet on Ag(111) was studied by scanning tunneling microscopy and density functional theory calculations. It was observed that hydrogenation of silicene at room temperature results in a perfectly ordered γ-(3×3) superstructure. A theoretical model, which involves seven H atoms and rearranged buckling of Si atoms, was proposed and agrees with experiments very well. Moreover, by annealing to a moderate temperature, about 450 K, a dehydrogenation process occurs and the clean silicene surface can be fully recovered. Such uniformly ordered and reversible hydrogenation may be useful for tuning the properties of silicene as well as for controllable hydrogen storage.
Implementation of a new technology for point detection (2005)

General Dynamics ATP (GDATP) and Sionex Corporation (Sionex) are carrying out a cooperative development for a handheld chemical agent detector, being called JUNO, which will have lower false positives, higher sensitivity, and improved interference rejection compared with presently available detectors. This enhanced performance is made possible by the use of a new principle of ion separation called Differential Mobility Spectrometry (DMS). The enhanced selectivity is provided by the field tunable nature of the Sionex differential mobility technology (microDMxTM) which forms the analytical heart of the JUNO system and enables fingerprinting of molecules by characterization of the ionized molecular behavior under multiple electric field conditions. This enhanced selectivity is valuable in addressing not only the traditional list of chemical warfare agents (CWA) but also the substantial list of Toxic Industrial Compounds (TICs) and Toxic Industrial Materials (TIMs) which may be released in warfare or terrorist situations. Experimental results showing the ability of the microDMx to reject interferences, detect and resolve live agents are presented. An additional breakthrough in the technology was realized by operating the device at a reduced pressure of around 0.5 atmospheres. This reduced pressure operation resulted in roughly doubling the spectrometers resolution over what has previously been reported [1]. Advances have also been made in power consumption and packaging leading to a device suitable for portable, handheld, applications. Experimental results illustrating the performance of the microDMx technology employed in JUNO are highlighted.
Skin Disposition of Drugs after Topical Application in Hairless Rats. (1999)

Drug fraction transported from a topical formulation on skin to subsutaneous tissues or muscles is dependent on the physicochemical properties of the entrapped drug. Cutaneous disposition of model drugs, antipyrine(ANP), lidocaine (LC) and piroxicam (PXC) as well as flurbiprofen (FP) was thus evaluated in hairless rats in which an agar gel disc was subcutaneously inserted into abdominal region as a drug receptor and a drug donor cell was placed above it. Time courses of plasma level and agar gel amount were measured after topical application of 50% ANP, 3% LC, 1% PXC and 1% FP in hydroxypropylcellulose gel. Percutaneous absorption clearance of unionized from, CL*ab was proportional to true octanol/water distribution coefficient and the order of FP>PXC>LC>ANP, suggesting that permeation of the drug was determined mainly by its distribution from the formulation to the skin barrier. PXC, however, had a relatively low flux compared to the other three drugs, probably due to its high molecular weight and meltiong point. Migration clearance of unionized form from systemic cirsulation to the subcutaneous agar gel, CL*g was also influenced by the lipophilicity of drugs. On the other hand, fraction from the formulation to the systemic circulation was in the order of PXC>FP>ANP>LC.This fraction was much higher than the direct migration fraction from the formulation to the subcutaneous agar gel. Factors determining for these fractions are still unclear. A drug having a low lipophilicity and a low prorein binding, however, had a tendency to have a great targeting ability to the subcutaneous agar gel. In addition, most of the drug in the agar gel was contributed by the direct flow from formulation, not from the systemic circulation. The present in situ experimental method is a useful tool to evaluate skin disposition of drugs. Detailed understanding of the skin disposition of drugs from several formulations will enable the finding of a good drug and formulation candidates.
The Iris Challenge Evaluation 2005 (2008)

This paper describes the Iris Challenge Evaluation (ICE) 2005. The ICE 2005 contains a dataset of 2953 iris images from 132 subjects. The data is organized into two experiments: right and left eye. Iris recognition performance is presented for twelve algorithms from nine groups that participated in the ICE 2005. For the top performers, verification rate on the right iris is above 0.995 at a false accept rate of 0.001. For the left iris, the corresponding verification rates are between 0.990 and 0.995 at a false accept rate of 0.001. The results from the ICE 2005 challenge problem were the first to observe correlations between the right and left irises for match and non-match scores, and quality measures.
Orexin Receptors Are Expressed in the Adrenal Medulla of the Rat (1999)

Two recently discovered hypothalamic peptides, orexin-A and orexin-B, play a role as mediators in the central mechanisms that regulate feeding behavior and sleep control. These peptides bind and activate two orexins receptors that belong to the G-protein coupled receptor superfamily. Morphological studies have detected mRNA expression of orexin receptors exclusively in the rat central nervous system. In this paper we demonstrate a strong level of expression of orexin receptor 1 and 2 in the adrenal medulla of the rat by RT-PCR and immunohistochemistry. The results of the present study provide the first evidence showing that the adrenal medulla expresses orexin receptors, and thus appears to be a target tissue for orexins. This could open a new loop in which the central and autonomous nervous system may be involved in body weight homeostasis and sleep control.
Detection of methanotrophic bacteria in environmental samples with the PCR (1995)

We designed PCR primers by using the DNA sequences of the soluble methane monooxygenase gene clusters of Methylosinus trichosporium OB3b and Methylococcus capsulatus (Bath), and these primers were found to be specific for four of the five structural genes in the soluble methane monooxygenase gene clusters of several methanotrophs. We also designed primers for the gram-negative methylotroph-specific methanol dehydrogenase gene moxF. The specificity of these primers was confirmed by hybridizing and sequencing the PCR products obtained. The primers were then used to amplify methanotroph DNAs in samples obtained from various aquatic and terrestrial environments. Our sequencing data suggest that a large number of different methanotrophs are present in peat samples and also that there is a high level of variability in the mmoC gene, which codes for the reductase component of the soluble methane monooxygenase, while the mmoX gene, which codes for the alpha subunit of the hydroxylase component of this enzyme complex, appears to be highly conserved in methanotrophs.
Incorporation of titanium dioxide particles into polymer matrix using block copolymer micelles for fabrication of high refractive and transparent organic–inorganic hybrid materials (2010)

Abstract We report a novel strategy for incorporation of titanium dioxide (TiO 2 ) particles into poly(methyl methacrylate) (PMMA) to exploit high refractive and transparent organic–inorganic hybrid materials. Formation of TiO 2 particles of around 20 nm was conducted within hydrophilic core of block copolymer micelles of poly(methyl methacrylate‐ block ‐acrylic acid) (PMMA‐ b ‐PAA) in toluene via sol–gel process from titanium isopropoxide and hydrochloric acid. Subsequently, incorporation of TiO 2 particles into PMMA matrix was carried out by casting toluene solution of TiO 2 precursor‐loaded copolymer micelles, prepared from PMMA 350 ‐ b ‐PAA 93 and the precursor of mole ratio Ti 4+ /carboxyl 4.0, and PMMA. Hybrid films of TiO 2 /PMMA exhibited high transparency to achieve transmission over 87% at 500 nm at 30 wt % of TiO 2 content. The refractive index of resulting hybrid films at 633 nm linearly increased with TiO 2 content to attain 1.579 at 30 wt % TiO 2 , which was 0.1 higher than that of PMMA. Cross‐sectional transmission electron microscope images of TiO 2 /PMMA hybrid films showed existence of TiO 2 clusters less than 100 nm, which were probably formed by aggregation or agglutination of TiO 2 particles during a drying process. It was also observed that decomposition temperature of the hybrid films elevated with increasing TiO 2 content. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
Three-dimensional MHD modeling of waves in active region loops (2008)

Abstract Observations show that MHD waves are one of the most important universal processes in the heliosphere. These waves are likely to play an important role in energy transfer in the heliosphere, and they can be used as a diagnostic tool of the properties of the local magneto-fluid environment. Recent observations by TRACE and Hinode satellites provide ample evidence of oscillations in coronal active region loops. The oscillations were interpreted as fast (kink), slow, and Alfvén modes, and the properties of the waves were used for coronal seismology. However, due to the complex interactions of the various modes in the inhomogeneous active region plasma, and due to nonlinearity, idealized linear theory is inadequate to properly describe the waves. To overcome this theoretical shortcoming we developed 3D MHD models of waves in active region loops. We investigated the effects of 3D active region magnetic and density structure on the oscillations and the wave dissipation, and we investigated the oscillation of individual loops. Some loops were constructed to contain several threads and twist. Here, we present the results of our models, and show how they can be used to understand better the properties of the waves, and of the active regions.
Emergence of drug-resistant pneumococcal infections in the United States (1994)

<h3>Objective.</h3> —To estimate drug susceptibility patterns of<i>Streptococcus pneumoniae</i>in selected hospitals in the United States and to characterize the epidemiology of invasive drug-resistant pneumococcal infections. <h3>Design.</h3> —Minimum inhibitory concentrations (MICs) for a variety of commonly used antimicrobial drugs were determined for pneumococcal isolates submitted to the Centers for Disease Control and Prevention (CDC). Risk factors for drug-resistant pneumococcal infection were evaluated. <h3>Setting.</h3> —Hospital laboratories in the United States submitting pneumococcal isolates to the CDC between October 1, 1991, and September 30, 1992. <h3>Participants.</h3> —A total of 544 persons with pneumococci isolated from normally sterile sites. <h3>Results.</h3> —A total of 13 hospitals in 12 states actively participated in an ongoing pneumococcal surveillance study. Resistance to penicillin was detected in 6.6% of isolates, including 1.3% of isolates with MICs of 2.0 μg/mL or more (compared with <0.02% of isolates with MIC ≥2.0 μg/mL identified by CDC surveillance from 1979 to 1987). A total of 16.4% were resistant to at least one of the following drugs or drug classes: penicillin, cephalosporins, macrolides, combination trimethoprim and sulfamethoxazole, and chloramphenicol. Six serotypes (6B, 23F, 14, 9V, 19A, and 19F) accounted for nearly 85% of strains resistant to at least one drug class. Children were more likely than adults to be infected with strains resistant to trimethoprim-sulfamethoxazole, erythromycin, or chloramphenicol. <h3>Conclusions.</h3> —Emergence of drug-resistant pneumococcal infections will present critical challenges to clinicians for treating patients with pneumococcal disease. Widened and intensified surveillance is needed. These data suggest that current recommendations for use of 23-valent pneumococcal capsular polysaccharide vaccines should be aggressively promoted and that development and evaluation of new conjugate pneumococcal vaccines may be a crucial part of strategies for prevention. (<i>JAMA</i>. 1994;271:1831-1835)
Volatile FPGA design security { a survey (2008)

Volatile FPGAs, the dominant type of programmable logic devices, are used for space, military, automotive, and consumer electronics applications which require them to operate in a wide range of environments. Their continuous growth in both capability and capacity now requires significant resources to be invested in the designs that are created for them. This has brought increased interest in their security attributes; specifically, how well does the FPGA protect the information processed within it, how are FPGA designs protected during distribution, and how developers’ ownership rights are protected while designs from multiple sources are combined. This survey, which assumes very basic logic design and security understanding, establishes the foundations for discussing “FPGA security”, examines a wide range of attacks and defenses along with the current state of industry offerings, and finally, outlines on-going research and latest developments. 1
Concentrations and Total Daily Output of Micronutrients in Breast Milk of Mothers Delivering Preterm (1983)

The number of preterm infants fed their own mother's milk appears to be increasing as a result of information suggesting certain benefits over milk from human milk banks or proprietary formulas. It is also apparent that the nutritional requirements of term and preterm infants differ. Thus, the finding of various deficiencies in small preterm infants indicates that studies which examine the nutrient content of milk from mothers who deliver prematurely are important to aid in suggesting appropriate supplementation. In order to provide appropriate recommendations, nutrient content must be assayed during the period of exclusive milk feedings. Accordingly, the concentrations and total daily output of breast milk zinc and copper, and vitamins A, C, and E, were examined in a group of 13 mothers delivering at or before 32 weeks gestation. Collections of a total 24-h output were obtained every 7 days for the first 7 weeks after delivery. Concentrations of all micronutrients were similar to those reported previously during the first 5 weeks of lactation. Most striking, however, was the progressive decline in total output as well as concentration of zinc. After the first 2 weeks of lactation, levels of copper and vitamins A, C, and E were not affected by the duration of lactation. There was substantial variation between mothers in daily output of all the micronutrients. The low output of these micronutrients suggests that some attempt should be made at monitoring the mineral and vitamin status of small preterm infants fed exclusively human milk for the first 3 months of life.(ABSTRACT TRUNCATED AT 250 WORDS)
Furanones, potential agents for preventing Staphylococcus epidermidis biofilm infections? (2008)

Furanones may inhibit biofilm formation through interference with quorum sensing and thus represent promising agents for protecting surfaces from being colonized by S. epidermidis.
Corrosion behaviors of zinc and Zn-Ni alloy compositionally modulated multilayer coatings (2005)

Zinc and Zn-Ni alloy compositionally modulated multilayer (CMM) coatings were electrodeposited from dual baths. The coated samples were evaluated in terms of surface appearance, surface and cross-sectional morphologies, as well as corrosion resistance. The results obtained from the salt spray test show that the zinc and Zn-Ni alloy CMM coatings are more corrosion-resistant than the monolithic coatings of zinc or Zn-Ni alloy alone with a similar thickness. The corrosion potential measurement and anodic polarisation tests were undertaken to examine the probable corrosion mechanisms of zinc and Zn-Ni alloy CMM coatings. Analysis on the micrographic features of zinc and Zn-Ni alloy CMM coatings after the corrosion test explains the probable reasons why the Zn-Ni/Zn CMM coatings have a better protective performance. Surface morphologies and compositional analysis of the remaining coating material of Zn-Ni alloy deposit after the corrosion test confirms the dezincification mechanism of the Zn-Ni alloy deposit during the corrosion process.
Direct Randomized Benchmarking for Multiqubit Devices (2019)

Benchmarking methods that can be adapted to multiqubit systems are essential for assessing the overall or "holistic" performance of nascent quantum processors. The current industry standard is Clifford randomized benchmarking (RB), which measures a single error rate that quantifies overall performance. But, scaling Clifford RB to many qubits is surprisingly hard. It has only been performed on one, two, and three qubits as of this writing. This reflects a fundamental inefficiency in Clifford RB: the n-qubit Clifford gates at its core have to be compiled into large circuits over the one- and two-qubit gates native to a device. As n grows, the quality of these Clifford gates quickly degrades, making Clifford RB impractical at relatively low n. In this Letter, we propose a direct RB protocol that mostly avoids compiling. Instead, it uses random circuits over the native gates in a device, which are seeded by an initial layer of Clifford-like randomization. We demonstrate this protocol experimentally on two to five qubits using the publicly available ibmqx5. We believe this to be the greatest number of qubits holistically benchmarked, and this was achieved on a freely available device without any special tuning up. Our protocol retains the simplicity and convenient properties of Clifford RB: it estimates an error rate from an exponential decay. But, it can be extended to processors with more qubits-we present simulations on 10+ qubits-and it reports a more directly informative and flexible error rate than the one reported by Clifford RB. We show how to use this flexibility to measure separate error rates for distinct sets of gates, and we use this method to estimate the average error rate of a set of cnot gates.
The immediate cause of the diurnal changes of nitrogen metabolism in leaves of nitrate-replete tobacco: a major imbalance between the rate of nitrate reduction and the rates of nitrate uptake and ammonium metabolism during the first part of the light period (2001)

To assess how diurnal changes of nitrate reductase (NIA) expression in leaves interact with upstream and downstream processes during nitrate utilization, nitrate uptake, and nitrate and ammonium metabolism were investigated at several times during the diurnal cycle in wild-type tobacco. Plants were grown hydroponically on 2 mM nitrate to exclude possible complications due to changes in the external availability of nitrate, and to allow nitrate uptake to be measured in the growth conditions. (a) In leaves, the NIA transcript decreases during the day and recovers at night, and NIA activity increases three-fold during the first part and declines during the second part of the light period. Nitrate decreases during the day and recovers at night, ammonium, glutamine, glycine and serine increase during the day and decrease at night, and 2-oxoglutarate increases three-fold after illumination and decreases during the last part of the light period. The amplitudes of the diurnal changes are similar to or larger than in tobacco grown on high nitrate in sand. The transcript for plastid glutamine synthetase (GLN2) is low at the end of the night and increases during the day, and glutamine synthetase activity increases to a peak at the end of the day and decreases at night. (b) In the roots, transcript levels for the high affinity nitrate transporter (NRT2) increase in the day and decrease at night. Nitrate uptake is about 40% higher during the day than at night. (c) Comparison of the diurnal changes of the leaf metabolite pools with the rate of nitrate uptake allows diurnal changes in fluxes to be estimated. During the first part of the light, the rate of nitrate assimilation is about two-fold higher than the rate of nitrate uptake, and also exceeds the rate at which reduced nitrogen is metabolized in the GOGAT pathway. The resulting decrease of leaf nitrate and accumulation of nitrogen in intermediates of ammonium metabolism and photorespiration represent about 40 and 15%, respectively, of the total nitrate that enters the plant in 24 h. Later in the diurnal cycle as NIA expression and activity decline, this imbalance is reversed. NRT2 expression and nitrate uptake remain relatively high, and nitrate taken up during the night is used to replenish the leaf nitrate pool. Increased GLN2 expression in leaves during the second part of the light period allows continued assimilation of ammonium released during photorespiration and remobilization of the reduced nitrogen that accumulated earlier in the diurnal cycle.