gebhart/patrepeval huggingface dataset:
Erythroid Gene Suppression by NF-κB (2003)

NF-kappa B/Rel transcription factors play essential roles to mediate the immune response and apoptosis, and they have also been implicated in cellular differentiation such as erythropoiesis. To elucidate the possible role(s) of NF-kappa B in erythroid gene regulation and erythropoiesis, we have carried out transient transfection studies of the human embryonic/fetal erythroid cell line K562 and mouse adult erythroid MEL cells. It is shown that tumor necrosis factor-alpha represses the transcription activity directed by either alpha or zeta globin promoter in a dose-dependent manner. Furthermore, different NF-kappa B family members could effectively repress the transfected alpha-like globin promoters in K562 as well as in MEL cells. The involvement of NF-kappa B pathway is supported by the ability of a NF-kappa B-specific, dominant negative mutant to block the tumor necrosis factor-alpha or p65-mediated suppression of the alpha-like globin promoter activities. The suppression appears to be mediated through cis-linked HS-40 enhancer. Finally, stably transfected K562 cells overexpressing p65 contain reduced amounts of the p45/NF-E2 RNA and functional NF-E2 proteins. Our studies have identified a new set of targets of NF-kappa B. We suggest that the relatively high activity of the NF-kappa B pathway in early erythroid progenitors is involved in the suppression of erythroid-specific genes. Later in differentiation, together with other changes, the decline of the amounts of the NF-kappa B family of factors leads to derepression and consequent increase of NF-E2, which in turn would activate a subset of erythroid-specific genes.
Application of the Analogy Between Water Flow with a Free Surface and Two-dimensional Compressible Gas Flow (1947)

From Introduction: "The development of the measuring apparatus and techniques is presented herein. The application of the analogy to flows through nozzles and about circular cylinders at subsonic velocities extending into supercritical range is also presented."
Multiple‐image radiography for human soft tissue (2006)

Conventional radiography only provides a measure of the X-ray attenuation caused by an object; thus, it is insensitive to other inherent informative effects, such as refraction. Furthermore, conventional radiographs are degraded by X-ray scatter that can obscure important details of the object being imaged. The novel X-ray technology diffraction-enhanced imaging (DEI) has recently allowed the visualization of nearly scatter-free images displaying both attenuation and refraction properties. A new method termed multiple-image radiography (MIR) is a significant improvement over DEI, corrects errors in DEI, is more robust to noise and produces an additional image that is entirely new to medical imaging. This new image, which portrays ultra-small-angle X-ray scattering (USAXS) conveys the presence of microstructure in the object, thus differentiating homogeneous tissues from tissues that are irregular on a scale of micrometres. The aim of this study was to examine the use of MIR for evaluation of soft tissue, and in particular to conduct a preliminary investigation of the USAXS image, which has not previously been used in tissue imaging.
MASSIVE REPOPULATION OF RAT LIVER BY TRANSPLANTATION OF HEPATOCYTES INTO SPECIFIC LOBES OF THE LIVER AND LIGATION OF PORTAL VEIN BRANCHES TO OTHER LOBES1 (1997)

An important consideration in application of hepatocyte transplantation is whether the number of engrafted hepatocytes is sufficient to achieve the desired effect. Here we have evaluated the proliferative potential of transplanted primary hepatocytes during regeneration of hepatic lobes. Two million hepatocytes isolated from congeneic normal Wistar-RHA rats were injected into the main portal vein of deficient, jaundiced Gunn rats. The right branch of the portal vein was ligated 24 hr before hepatocyte transplantation (group A) or transiently clamped during hepatocyte injection (group B) or 24 hr after hepatocyte injection (group C). In these groups, the three lobes supplied by the right branch of the portal vein rapidly atrophied and disappeared in 4 days, whereas the remaining lobes proliferated, as shown by size increase and 5-bromo-2-deoxy-uridine uptake. Two control groups received 2 million (group D) or 20 million hepatocytes (group E) without ligation. Hepatocyte engraftment occurred in all groups. The greatest hypobilirubinemic effect was observed in group A, in which serum bilirubin concentrations were reduced to 1.7+/-0.45 mg/dl from pretransplantation levels of 6.9+/-1.2 mg/dl. This effect was even greater than that observed after transplantation of 20 times more hepatocytes without ligation (group E). Specific endonuclease digestion of a polymerase chain reaction-amplified segment of the ugt1 gene from hepatic DNA showed that up to 25% of the DNA was of donor origin. This paralleled the hepatic bilirubin-UDP-glucuronosyltransferase activity, which was above 50% of normal. The results indicate that the transplanted hepatocytes proliferate preferentially within the regenerating lobes, replacing more than 20% of the liver mass with the progeny of the transplanted phenotypically normal hepatocytes.
Selective Inhibition of Neuronal Nitric Oxide Synthase by <i>N</i><sup>ω</sup>-Nitroarginine- and Phenylalanine-Containing Dipeptides and Dipeptide Esters (1997)

A series of N omega-nitroarginine (ArgNO2)- and phenylalanine-containing dipeptides and dipeptide esters were synthesized as potential selective inhibitors of neuronal nitric oxide synthase (nNOS). All of the dipeptides and dipeptide esters are competitive inhibitors of nNOS, macrophage nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS), except for the ones that contain D-ArgNO2 (8-10, 12, 13), which are uncompetitive inhibitors of iNOS but competitive inhibitors of nNOS and eNOS. None of the dipeptides or dipeptide esters tested (1, 2, 12, 13) exhibited time-dependent inhibition of any of the NOS isoforms, unlike N omega-nitro-L-arginine itself, which does, although it is reversible. The order of the amino acids in the dipeptide or dipeptide ester is important to selectivity, and the selectivity depends on the chirality of the amino acids. In the case of the corresponding benzyl esters (5 vs 6), both dipeptides favor iNOS over nNOS and eNOS inhibition. All of the dipeptide methyl esters containing a D-amino acid, however, exhibit an inhibitory preference for nNOS over iNOS and eNOS. The most impressive selectivities observed are 1800- and 800-fold for 12 and 13, respectively, in favor of nNOS over iNOS; unfortunately, the selectivities of these compounds for nNOS over eNOS are only 2.5 and 5.3, respectively.
Coordinated Activation of Programmed Cell Death and Defense Mechanisms in Transgenic Tobacco Plants Expressing a Bacterial Proton Pump (1995)

Ron Mittler, Vladimir Shulaev, Eric Lam, Coordinated Activation of Programmed Cell Death and Defense Mechanisms in Transgenic Tobacco Plants Expressing a Bacterial Proton Pump, The Plant Cell, Vol. 7, No. 1 (Jan., 1995), pp. 29-42
Computational Model of the Cerebral Ventricles in Hydrocephalus (2010)

Understanding the mechanisms of tissue injury in hydrocephalus is important to shed light on the pathophysiology of this neurostructural disorder. To date, most of the finite element models created to study hydrocephalus have been two-dimensional (2D). This may not be adequate as the geometry of the cerebral ventricles is unique. In this study, a three-dimensional (3D) finite element model of the cerebral ventricles during hydrocephalus is presented. Results from this model show that during hydrocephalus, the periventricular regions experience the highest stress, and stress magnitude is approximately 80 times higher than the cerebral mantle. This suggests that functional deficits observed in hydrocephalic patients could therefore be more related to the damage to periventricular white matter. In addition, the stress field simulated in the tissues based on the 3D model was found to be approximately four times lower than on the 2D model.
A Differentially Excited Coupled Half-Width Microstrip Leaky ${\rm EH}_{1}$ Mode Antenna (2013)

This paper presents a coupled half-width leaky-wave antenna driven differentially. The guided-wave structure of coupled half-width microstrip lines are proposed and investigated, showing two leaky modes in the form of even- and odd-symmetry, respectively. Rigorous studies show that the odd-leaky-mode outperforms the even-mode approach in radiation patterns and bandwidth. Following the rigorous investigation of the modal characteristics of the coupled half-width EH <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> mode guided structure, this paper reports a differentially fed, coupled, odd-leaky-mode, half-width, antenna of size 1λ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> by 3.6λ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> (including the feed), showing measured maximum antenna gain of 11.6 dBi, 15% fractional bandwidth, gain flatness of 2.4-dB variation, and input return loss greater than 10 dB. (λ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> is the free space wavelength at the maximum gain frequency of 10.1 GHz). Both full-wave simulated results and measured data show good agreements for the proposed antenna structure. The measured leaky-wave antenna performance is compared to the recently published results, demonstrating a good compromise among all major performance metrics.
Self-diffusion of particles in shear flow of a suspension (1977)

Self-diffusion coefficients were determined experimentally for lateral dispersion of spherical and disk-like particles in linear shear flow of a slurry at very low Reynolds number. Using a concentric-cylinder Couette apparatus, recurrent observations were made of the lateral position of a particular radioactively labelled particle. The self-diffusion coefficient D was calculated by means of random-walk theory, using the ergodic hypothesis. Owing to great experimental difficulties, the calculated values of D are not of high accuracy, but are correct to within a factor of two. In the range 0 < ϕ < 0·2, D/a 2 ω increases from zero linearly with ϕ up to D/a 2 ω ≅ 0·02 (where ϕ = volumetric concentration of particles, a = particle radius, ω = mean shear rate of suspending fluid). In the range 0·2 < 0·5, the trend of D/a 2 ω is not clear because of experimental scatter, but in this range D/a 2 ω ≅ 0·025 to within a factor of two. Within the experimental accuracy, spheres and disks have the same value of D/a 2 ω.
Control of Orientation of Pb(Zr, Ti)O<sub>3</sub> Thin Films Using PbTiO<sub>3</sub> Buffer Layer (1994)

The orientation of Pb(Zr, Ti)O 3 (PZT) thin films grown by metalorganic chemical vapor deposition (MOCVD) using a two-step growth process was controlled by changing the orientation of the PbTiO 3 buffer layer. The (111)-oriented and (100)-oriented PZT films were grown on the (111)-oriented and the (001) and (100) mixed-oriented PbTiO 3 buffer layers, respectively. From the atomic force microscope (AFM) observations, it was found that in the initial growth stages of the (111)-oriented PbTiO 3 layer, the main growth mechanism was two-dimensional growth. In the two-step growth process, the PbTiO 3 buffer layer played the important role of providing dense nuclei in the succeeding growth of rhombohedral PZT thin films. The influence of the thickness of a buffer layer on the relative dielectric constants of PZT films was also investigated.
Structural Requirements for Activation of Latent Platelet-derived Growth Factor CC by Tissue Plasminogen Activator (2005)

Platelet-derived growth factor C (PDGF-C) is one of four members in the PDGF family of growth factors, which are known mitogens and survival factors for cells of mesenchymal origin. PDGF-C has a unique two-domain structure consisting of an N-terminal CUB and a conserved C-terminal growth factor domain that are separated by a hinge region. PDGF-C is secreted as a latent dimeric factor (PDGF-CC), which undergoes extracellular removal of the CUB domains to become a PDGF receptor alpha agonist. Recently, the multidomain serine protease tissue plasminogen activator (tPA), a thrombolytic agent used for treatment of acute ischemic stroke, was shown to cleave and activate PDGF-CC. In this study we determine the molecular mechanism of tPA-mediated activation of PDGF-CC. Using various PDGF-CC and tPA mutants, we were able to demonstrate that both the CUB and the growth factor domains of PDGF-C, as well as the kringle-2 domain of tPA, are required for the interaction and cleavage to occur. We also show that Arg231 in PDGF-C is essential for tPA-mediated proteolysis and that the released "free" CUB domain of PDGF-C can act as a competitive inhibitor of the cleavage reaction. Furthermore, we studied how the PDGF-C/tPA axis is regulated in primary fibroblasts and found that PDGF-C expression is down-regulated by hypoxia but induced by transforming growth factor (TGF)-beta1 treatment. Elucidating the regulation and the mechanism of tPA-mediated activation of PDGF-CC will advance our knowledge of the physiological function of PDGF-CC and tPA and may provide new therapeutic opportunities for thrombolytic and cardiovascular therapies.
Power Doppler versus color Doppler imaging in renal allograft evaluation. (1996)

The purpose of this study was to evaluate the impact of PDS compared to conventional CDI in the followup of 72 renal allograft patients. Renal allograft vascularization, assessed by PDS and CDI, was scored from 0 to 4, where 0 was the cortical "blush" and 4 was residual central perfusion. These scores were correlated with the resistive index, serum creatinine levels, hematocrit, and, in 35 cases, biopsy results. PDS scores of renal perfusion were one grade lower than CDI scores in 59 of 72 patients and two grades lower in two of 72 patients. A statistically significant correlation was found between PDS scores and the RI (r2 = 0.6, P < 0.05). However, no significant correlation was found between PDS scores and creatinine levels or hematocrit values. PDS scores are not related to histologic findings in renal allograft dysfunction. Overall, five biopsy-related arteriovenous fistulas were detected, two of which were missed on the initial PDS examination. In conclusion, PDS provides more complete visualization of the renal allograft vessel tree than CDI. However, biopsy-related arteriovenous fistulas are better seen by CDI.
BIOACTIVE COMPOUNDS IN NUTRITION AND HEALTH-RESEARCH METHODOLOGIES FOR ESTABLISHING BIOLOGICAL FUNCTION: The Antioxidant and Anti-inflammatory Effects of Flavonoids on Atherosclerosis (2004)

Identifying bioactive compounds and establishing their health effects are active areas of scientific inquiry. There are exciting prospects that select bioactive compounds will reduce the risk of many diseases, including chronic diseases such as cardiovascular disease. Recent findings have established that cardiovascular disease is a disease of inflammation, and consequently is amenable to intervention via molecules that have anti-inflammatory effects. In addition, research demonstrating adverse effects of oxidants on atherogenesis raises the possibility that antioxidants can confer cardioprotective effects. This review provides an overview of research approaches that can be used to unravel the biology and health effects of bioactive compounds. Because of the number of bioactive compounds and the diversity of likely biological effects, numerous and diverse experimental approaches must be taken to increase our understanding of the biology of bioactive compounds. Recognizing the complexity of this biology, sophisticated experimental designs and analytical methodologies must be employed to advance the field. The discovery of novel health effects of bioactive compounds will provide the scientific basis for future efforts to use biotechnology to modify/fortify foods and food components as a means to improve public health.
Acute Renal Failure: Diagnosis, Treatment, and Prevention (1991)

Thirty-four papers from the Symposium on Acute Renal Failure, held in September 1988 (place unspecified), emphasize in particular: areas where investigations of pathogenesis in experimental systems could be applied to develop new clinical strategies for treatment and prevention of acute renal failur
Efficiency and Evolution of Enzyme Catalysis (1977)

Abstract A new function derived from kinetic data, the efficiency function, can be used to quantify the efficiency of a catalyst. For freely diffusing species the maximum efficiency is unity. The enzyme triose phosphate isomerase has an efficiency of 0.6 and is thus almost a perfect catalyst. The efficiency of the acetate ion as catalyst for the same reaction is 2.5 × 10 −11 . This increase in catalytic efficiency is discussed in terms of three types of evolutionary improvement: the uniform binding of the substrate to the enzyme, changes in the internal thermodynamics of the bound states, and more effective catalysis of elementary steps. These concepts are illustrated for triose phosphate isomerase.
Interactive movie system with multi-person participation and anytime interaction capabilities (1998)

Article Interactive movie system with multi-person participation and anytime interaction capabilities Share on Authors: Ryohei Nakatsu ATR Media Integration and Communications Research Laboratories, 2-2 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0288, Japan ATR Media Integration and Communications Research Laboratories, 2-2 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0288, JapanView Profile , Naoko Tosa ATR Media Integration and Communications Research Laboratories, 2-2 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0288, Japan ATR Media Integration and Communications Research Laboratories, 2-2 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0288, JapanView Profile , Takeshi Ochi ATR Media Integration and Communications Research Laboratories, 2-2 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0288, Japan ATR Media Integration and Communications Research Laboratories, 2-2 Hikaridai, Seika-cho, Soraku-gun, Kyoto 619-0288, JapanView Profile Authors Info & Claims MULTIMEDIA '98: Proceedings of the sixth ACM international conference on MultimediaSeptember 1998 Pages 129–137https://doi.org/10.1145/290747.290764Online:01 September 1998Publication History 3citation389DownloadsMetricsTotal Citations3Total Downloads389Last 12 Months12Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Static leakage reduction through simultaneous threshold voltage and state assignment (2003)

We propose a new method that uses a combined approach of sleep-state assignment and threshold voltage (Vt) assignment in a dual-Vt process. While each of these methods has previously been used individually, their combined effect has not been leveraged to date. By combining Vt and sleep-state assignment, leakage current can be dramatically reduced since the circuit is in a known state in standby-mode and only transistors that are off need to be considered for high-Vt assignment. A significant improvement in the leakage/ performance trade-off is therefore achievable using such a combined method. We formulate the optimization problem for simultaneous state and Vt assignment under delay constraints and propose both an exact method for its optimal solution as well as a number of practical heuristics with reasonable run time. We compare our results with Vt and sleep state assignment only and demonstrate an average decrease in leakage current of 3.5X compared to previous approaches.
The Design of Reflecting Microscope Objectives (1951)

The general theory is developed and the exact solutions obtained for the design of reflecting microscope objectives having two or three concentric spherical surfaces. It is shown that the only serious optical defect of reflecting objectives, low contrast due to the high obstruction ratio, can be satisfactorily controlled in three-mirror systems. Non-concentric objectives are discussed, but in practice prove to be difficult to adjust.
Development of Lentiviral Vectors with Optimized Transcriptional Activity for the Gene Therapy of Patients with Fanconi Anemia (2009)

Fanconi anemia (FA) is an inherited genetic disease characterized mainly by bone marrow failure and cancer predisposition. Although gene therapy may constitute a good therapeutic option for many patients with FA, none of the clinical trials so far developed has improved the clinical status of these patients. We have proposed strategies for the genetic correction of bone marrow grafts from patients with FA, using lentiviral vectors (LVs). Here we investigate the relevance of the expression of FANCA to confer a therapeutic effect in cells from patients with FA-A, the most frequent complementation group in FA. Our data show that relatively weak promoters such as the vav or phosphoglycerate kinase (PGK) promoter confer, per copy of FANCA, physiological levels of FANCA mRNA in lymphoblastoid cell lines, whereas the cytomegalovirus and, more significantly, spleen focus-forming virus (SFFV) promoters mediated the expression of supraphysiological levels of FANCA mRNA. Insertion of the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) or a mutated WPRE into the 3' region of PGK-FANCA LVs significantly increased FANCA mRNA levels. At the protein level, however, all tested vectors conferred, per copy of FANCA, similar and physiological levels of the protein, except SFFV LVs, which again conferred supraphysiological levels of FANCA. In spite of their different activity, all tested vectors mediated a similar phenotypic correction in FA-A lymphoblastoid cell lines and also in hematopoietic progenitors from patients with FA-A. On the basis of the efficacy and safety properties of PGK LVs, a PGK LV carrying FANCA and a mutant WPRE is proposed as an optimized vector for the gene therapy of patients with FA-A.
Exchanging Data Mining Models with the Predictive Modelling Markup Language (2001)

The aim of the Predictive Model Markup Language (PMML) is to support the exchange of data mining models between different applications and visualization tools. It is the result of a standardization effort by a group of vendors. PMML is an XML-based language (grammar) for describing data mining models. Despite its name, it is not limited to predictive models. The contribution of this paper is two-fold: an encouragement for researchers to base their work on PMML and a slightly enhanced PMML DTD for multi-relational rule models.
Acoustic helium analyzer for closing volume measurement (1977)

An acoustic gas analyzer was developed to measure helium during the closing volume (CV) test. It is based on the principle that altering the composition of a gas mixture will change the velocity at which sound is transmitted through the gas. The change in transit time for an acoustic burst across an in-line (breathe-through) cell is measured as a function of He concentration in the expired air during the CV test. Although CO2 and H2O vapor affect the signal, so it is not specific for He, the system provides good records of CV. A comparison of measurements in 21 normal subjects demonstrated no differences for CV measured in the same breath by the bolus technique using the acoustic gas analyzer and by the resident gas method using a nitrogen analyzer. Differences in closing volumes measured by the He bolus technique and the resident gas method might exist in patients with some kinds of pulmonary disease, and the suggestion is made that CV measurements by the two methods might assist in describing pulmonary alterations in patients with bronchopulmonary disease.