gebhart/patrepeval huggingface dataset:
Cytopathogenic and Noncytopathogenic RNA Replicons of Classical Swine Fever Virus (1999)

To determine the minimal requirements for autonomous RNA replication of classical swine fever virus (CSFV), genomes having in-frame deletions within the genes for structural and flanking nonstructural proteins were constructed, based on an infectious cDNA clone of CSFV Alfort/187. RNA was transcribed in vitro from the respective plasmids and transfected into SK-6 swine kidney cells. The replication competence of the RNA was determined by immunostaining transfected cells for CSFV NS3 protein and by analysis of cell extracts for viral RNA, as well as protein synthesis at different times after transfection. The genes encoding N(pro), C, E(rns), E1, E2, p7, and NS2 proved to be dispensable for RNA replication, but the efficiency of replication varied strongly between individual constructs. RNA replicons containing the complete NS2-NS3 gene persisted in transfected cells and continued to replicate without causing any obvious morphological or functional damage to the cells, whereas genomes lacking the NS2 gene replicated more efficiently and induced a cytopathic effect. These findings suggest that NS2, although it is not essential for pestivirus RNA replication, has a regulatory function therein. Both cytopathogenic and noncytopathogenic replicons were packaged into virus particles provided in trans by a cotransfected full-length helper virus genome.
Bovine herpesvirus 1 U(s) open reading frame 4 encodes a glycoproteoglycan (1996)

Sequence analysis of the short unique (Us) segment of the bovine herpesvirus 1 (BHV-1) genome predicted that the Us open reading frame (ORF) 4 encodes a protein with homology to glycoprotein G (gG) of other alpha-herpesviruses (P. Leung-Tack, J.-C. Audonnet, and M. Riviere, Virology 199:409-421, 1994). RNA analysis showed that the Us ORF4 is contained within two transcripts of 3.5 and 1.8 kb. The 3.5 kb RNA represents a structurally bicistronic RNA which encompasses the Us ORF3 and Us ORF4, whereas the 1.8-kb RNA constitutes the monocistronic Us ORF4 mRNA. To identify the predicted BHV-I gG, recombinant vaccinia virus expressing the Us ORF4 was used to raise specific antibodies in rabbits. The antiserum recognized a 65-kDa polypeptide and a very diffusely migrating species of proteins with an apparent molecular mass of between 90 and greater than 240 kDa in supernatants of BHV-1-infected cells which was also precipitated together with 61- and 70-kDa polypeptides from cell-associated proteins. The specificity of the reaction was demonstrated by the absence of these proteins from the supernatant of cells infected with the Us ORF4 deletion mutant BHV-l/gp1-8. Treatment of the immunoprecipitated proteins with glycosidases and chondroitinase AC showed that the 65-kDa protein constitutes gG, which contains both N- and O-linked carbohydrates, and that the high-molecular-mass proteins contain glycosaminoglycans linked to a 65-kDa glycoprotein that is antigenically related to gG. These molecules were therefore named glycoproteoglycan C (gpgG). Pulse chase experiments indicated that gG and gpgG were processed from a common precursor molecule with an apparent molecular mass of 61 kDa via a 70-kDa intermediate. Both gG and gpgG could not be found associated with purified virions. In summary, our results identify the BHV-I gG protein and demonstrate the presence of a form of posttranslational modification, glycosamino-glycosylation, that has not yet been described for a herpesvirus-encoded protein.
Introducing REXX into the engineering curriculum (2002)

The restructured extended executor language (REXX), developed by IBM, is a language developed with programmers in mind. It is an interpreted language rather than a compiled language, so it is relatively fast in execution. REXX is available on both mainframes and personal computers. REXX is being introduced at the undergraduate level in the Electrical Engineering Department at Southern University on an experimental basis. Program results are presented. It is shown that REXX is easy to learn. Menu driven programs and customized application programs are developed efficiently. Because external programs can be called by REXX, programs written by several programmers in different languages can be utilized to solve a given program.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Knowledge-enhanced Matching Pursuit (2013)

Compressive Sensing is possible when the sensing matrix acts as a near isometry on signals of interest that can be sparsely or compressively represented. The attraction of greedy algorithms such as Orthogonal Matching Pursuit is their simplicity. However they fail to take advantage of both the structure of the sensing matrix and any prior information about the sparse signal. This paper introduces an oblique projector to matching pursuit algorithms to enhance detection of a component that is present in the signal by reducing interference from other candidate components based on prior information about the signal as well as the structure of the sensing matrix. Numerical examples demonstrate that performance as a function of SNR is superior to conventional matching pursuit.
A TECHNIQUE USED ON MOUNDS OF <i>COPTOTERMES LACTEUS</i> TO SCREEN POTENTIAL BAIT SUBSTRATES (1985)

Abstract A simple method designed to rapidly screen potential bait substrates on mounds of Coptotermes lacteus (Froggatt) is described. The apparatus is cheap, readily prepared in the laboratory, and easily installed in the field.
G-protein-coupled Receptor Kinase-interacting Proteins Inhibit Apoptosis by Inositol 1,4,5-Triphosphate Receptor-mediated Ca2+ Signal Regulation (2009)

The inositol 1,4,5-trisphosphate (IP(3)) receptor (IP(3)R) is an intracellular IP(3)-gated calcium (Ca(2+)) release channel and plays important roles in regulation of numerous Ca(2+)-dependent cellular responses. Many intracellular modulators and IP(3)R-binding proteins regulate the IP(3)R channel function. Here we identified G-protein-coupled receptor kinase-interacting proteins (GIT), GIT1 and GIT2, as novel IP(3)R-binding proteins. We found that both GIT1 and GIT2 directly bind to all three subtypes of IP(3)R. The interaction was favored by the cytosolic Ca(2+) concentration and it functionally inhibited IP(3)R activity. Knockdown of GIT induced and accelerated caspase-dependent apoptosis in both unstimulated and staurosporine-treated cells, which was attenuated by wild-type GIT1 overexpression or pharmacological inhibitors of IP(3)R, but not by a mutant form of GIT1 that abrogates the interaction. Thus, we conclude that GIT inhibits apoptosis by modulating the IP(3)R-mediated Ca(2+) signal through a direct interaction with IP(3)R in a cytosolic Ca(2+)-dependent manner.
Signaling by the Cytokine Receptor Superfamily<sup>a</sup> (1998)

A bstract : A variety of cytokines that regulate functions of multiple lineages share the utilization of receptors that are structurally and functionally related and are referred to as the cytokine receptor superfamily. These receptors associate with one or more of the four mammalian Janus kinases (Jaks) and ligand‐induced receptor aggregation results in their activation. Critical roles for Jak3 and Jak2 are demonstrated by the phenotypes of mice that lack each gene. Among the substrates of the Jaks are one or more of the seven members of the signal transducers and activators of transcription (Stats). Each Stat family member plays a critical role in the biological functions of specific cytokines as demonstrated by the phenotype of mice lacking one or more of these genes.
The murine decorin. Complete cDNA cloning, genomic organization, chromosomal assignment, and expression during organogenesis and tissue differentiation. (1994)

Decorin, a proteoglycan known to interact with collagen and growth factors, may play key roles during ontogenesis, tissue remodeling, and cancer. We have deciphered the complete protein sequence of the murine decorin by cDNA cloning, elucidated its gene structure and chromosomal location, and investigated its expression in the developing embryo. The decorin protein and the gene were highly conserved vis à vis the human counterpart; however, the murine gene lacked a leader exon, exon Ib, which was found only in the human. Using interspecific backcrossing, we assigned the gene to chromosome 10 just proximally to the Steel gene locus. In situ hybridization studies of developing mouse embryos showed a distinct pattern of expression with a progressive increase of decorin mRNA during ontogenesis. At early stages (day 11 postconception), decorin was detectable only in the floor plate region. Subsequently (days 13-16 postconception), decorin expression was especially prominent in the meninges and mesothelial linings of pericardium, pleura, and coelomic cavity, as well as in the dermis and subepithelial layers of the intestine and urinary bladder. In contrast, the major parenchymal organs were only weakly positive for decorin mRNA. These findings suggest that decorin may play a role in epithelial/mesenchymal interactions during organ development and shaping.
Estradiol Down-Regulates the Mannose-6-Phosphate/Insulin-Like Growth Factor-II Receptor Gene and Induces Cathepsin-D in Breast Cancer Cells: A Receptor Saturation Mechanism to Increase the Secretion of Lysosomal Proenzymes (1991)

We have studied the regulation by estradiol of the mannose-6-phosphate (Man-6-P)/insulin-like growth factor-II (IGF-II) receptor concentration in different breast cancer cell lines. The mRNA level was assayed by Northern blot using the H5.1 cDNA probe. The protein level was assayed by Western ligand blot, by binding saturation with [125I]procathepsin-D on total membrane preparations, and by immunoprecipitation of 35S-labeled proteins. In three estrogen receptor-positive cell lines (MCF7, T47D, and ZR75-1), estradiol specifically decreased the steady state level of the Man-6-P/IGF-II receptor protein and mRNA. Moreover, in different cell lines and in primary culture of normal mammary cells, the secretion of procathepsin-D was inversely correlated with the level of Man-6-P/IGF-II receptor protein and mRNA. We conclude that estradiol down-regulates the Man-6-P/IGF-II receptor in breast cancer cells. Since two of its ligands, procathepsin-D and IGF-II, are induced by estrogen, we propose that the Man-6-P/IGF-II receptor becomes saturated after estrogen treatment. This model might explain the previously described estrogen-induced secretion of procathepsin-D and other lysosomal proenzymes routed by the same transport system.
An efficient eigenvector approach for finding netlist partitions (1992)

A fast eigenvector technique for obtaining good initial node partitions of netlists for use in interchange heuristics is described. The method is based on approximating the netlist or hypergraph by a weighted graph G, such that the sum of the cut edges in G tightly underestimates the number of cut nets in any netlist partition. An eigenvector technique is used to partition the graph G into k blocks of fixed module size. Another feature of this graph underestimation model of the netlist is that it allows one to obtain lower bounds on the actual number of cut nets. A multiblock node interchange heuristic is tested on the one resulting netlist partition obtained by this eigenvector approach on a variety of small to large sized benchmark netlist partitioning problems (between 300 to 12000 modules and nets). Test results on the larger netlists show that in most cases this eigenvector-node interchange approach yields netlist partitions with comparable or fewer cut nets than the best netlist partitions obtained by using node interchange heuristics alone on many random initial netlist partitions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Ataxia Telangiectasia: Genetics, Neuropathology, and Immunology of a Degenerative Disease of Childhood (1987)

This book begins with the laudable aim of providing "a comprehensive and easily understood book on genetic counseling for primary care physicians, nurse practitioners and social workers".I am sorry to say that, in my opinion, it fails to achieve this aim and cannot be recommended.The first five chapters cover basic genetics, reproductive options, and prenatal diagnosis.There follow 14 chapters on specific disorders, mostly organised along 'system' lines.The style is didactic and rather forbiddingly dogmatic.The chapter on inheritance patterns, for example, begins with a glossary and continues with a series of baldly stated rules.Many of the 'system' chapters are entitled
Metallocenes for Ethene/Propene Copolymerizations with High Product of Reactivity Ratios (1999)

Ethene/propene copolymerizations were performed with catalytic systems composed of a stereorigid racemic isospecific metallocene and polymethylalumoxane. A number of complexes were used as representatives of three different classes of metallocenes. r-Me2C(3-t-Bu-Cp)2ZrCl2, r-Me2C(3-t-Bu-Ind)2ZrCl2, r-EBDMIZrCl2, r-EBDMTHIZrCl2, and r-Me2Si(2-Me-4-Ph-Ind)2ZrCl2 were chosen as sterically hindered complexes. The parent metallocenes, r-Me2C(Ind)2ZrCl2, r-EBIZrCl2, and r-Me2Si(Ind)2ZrCl2 were also employed as well as other compounds bearing different substituents on the indenyl ligands such as r-Me2C(3-Me3Si-Ind)2ZrCl2, r/m-Me2C(3-Me-Ind)2ZrCl2, and r-Me2Si(2-Me-Ind)2ZrCl2. The reactivity ratios of copolymerization (rE and rP) were determined and examined in the light of the metallocene structure. The complexes that were particularly demanding from the steric point of view were identified as suitable candidates for promoting copolymerizations with a high product of reactivity ratios, giving rErP in a range from 1.7 to 3.0. The other two classes of metallocenes gave rise to rErP clearly lower than 1. A more complex situation was observed as far as the reactivity ratios rE and rP are concerned. The enhancement of the steric hindrance brought about in most cases an increase of rE but not a substantial decrease of rP. In some cases, rE was found to remain almost unchanged and rP dramatically increased. A correlation between the reactivity ratios and the metallocene structure is attempted.
Evaluation of intrarenal oxygenation by BOLD MRI at 3.0T (2004)

Higher magnetic field strength is beneficial for renal BOLD MRI studies. The cortico-medullary contrast on the R2* map was significantly improved at 3.0 T, with no evidence of increased bulk susceptibility artifacts. Baseline R2* and DeltaR2* in the renal medulla at 3.0 T were both significantly higher compared to our previously reported data obtained at 1.5 T.
Infrared Spectroscopy, Gas Chromatography/Infrared in Food Analysis (2000)

Abstract Infrared (IR) radiation is usually defined as that electromagnetic radiation whose frequency is between 20 and 14 500 cm −1 . Within this region of the electromagnetic spectrum, chemical compounds absorb IR radiation provided there is a dipole moment change during a normal molecular vibration, molecular rotation, and molecular rotation–vibration or from combinations, differences and overtones of the normal molecular vibrations. The frequencies and intensities of the IR bands exhibited by a chemical compound uniquely characterize the material and its IR spectrum can be used to identify and quantify a particular substance in an unknown sample. IR spectroscopy in both the mid‐infrared (MIR) and near‐infrared (NIR) regions is emerging as a qualitative as well as quantitative analytical technique with great potential in a wide range of applied areas. The prime advantage of IR spectroscopy in food applications is that once the instrument has been calibrated, several constituents in a sample can be measured rapidly and simultaneously. No sample manipulation is required. It is also applicable to on‐line analysis in the food processing industries.
Parallel Optical Transmitter Module Using Angled Fibers and a V-Grooved Silicon Optical Bench for VCSEL Array (2006)

We propose an advanced structure of optical subassembly (OSA) for packaging of the vertical-cavity surface-emitting laser (VCSEL) array, using (111) facet mirror of the V-groove ends formed in a silicon optical bench (SiOB) and angled fiber apertures. The feature of our OSA can provide a low optical crosstalk between neighboring channels, a low feedback reflection, and a large misalignment tolerance along the V-groove. We describe the optimized design of fiber angle, VCSEL position, and fiber position. The fabricated OSA structure consists of 12 channels of angled fiber array, 54.7deg V-grooves, Au-coated mirrors on (111) end facet of the V-grooves, and flip-chip-bonded VCSEL array on a SiOB. In this structure, the beam emitted from the VCSEL is deflected at the 54.7deg mirror of (111) end facet and propagated into the angled fiber. The angled fiber array was polished by 57deg. Fabricated OSAs showed a coupling efficiency of 30%-50% that is 25 times larger than that obtained from an OSA with a vertically flat fiber array. Our OSA showed large misalignment tolerance of about 90 mum along the longitudinal direction in the V-groove. We fabricated a parallel optical transmitter module using the OSA and demonstrated 12 channels times2.5 Gb/s data transmission with a clear eye diagram
Performance of shorted microstrip patch antennas for mobile communications handsets at 1800 MHz (1999)

We have investigated the application of two different types of novel shorted-patch antennas for mobile communications handsets at 1800 MHz. A single shorted-patch and a stacked shorted-patch antenna offering improved bandwidth are compared with data for a /spl lambda//4 monopole. The finite-difference time-domain (FDTD) technique was used to calculate antenna characteristics such as impedance and radiation patterns for two cases: on a handset and on a handset near a (2.5-mm voxel) heterogeneous head model in an actual position of phone use. We also obtained specific absorption rate (SAR) distributions and calculated the spatial peak 1-g SAR values. In addition, the effect on SAR and antenna characteristics of including a block model of the hand was assessed. Similar performance is achieved from the single or stacked shorted-patch antenna with the latter providing greater bandwidth, 8.2% versus 9.4% with the head and hand included. Both antennas reduce the l-g spatial peak SAR value in the head by 70% relative to the monopole. The presence of the hand reduces the efficiency of all three antenna types by approximately 10%.
Oxidative Dehydrogenation of Propane by Monomeric Vanadium Oxide Sites on Silica Support (2007)

We investigate possible mechanisms of oxidative dehydrogenation of propane using density functional theory. Monomeric vanadium oxide species supported on silica are modeled by vanadyl-substituted silsesquioxane. Similarly to other catalysts with transition metal oxo bonds, the initial C−H bond activation step is hydrogen abstraction by the vanadyl (OVV) group yielding a diradical intermediate in which a propyl radical is bound to a HO−VIV site. This is followed by a propyl rebound mechanism yielding alkoxide or alcohol attached to a VIII(OSi)3 surface site from which propene can be formed. Propene is also directly obtained by a second hydrogen abstraction from the diradical intermediate. Desorption of propyl radicals leads to a stationary concentration of propyl in the gas phase and leaves reduced HO−VIV sites on the surface. Due to fast reoxidation their concentration is much smaller than the concentration of OVV sites. Therefore the rate of propene formation after readsorption on OVV sites is much larger than the rate of isopropyl alcohol (or propene) formation after readsorption on HO−VIV sites. Generation of surface propyl radicals by the first hydrogen abstraction becomes rate limiting. We predict that at 750 K the apparent activation energy is 123 ± 5 kJ/mol and the rate constant is about 0.26 s-1, in close agreement with experiments. The first hydrogen abstraction occurs exclusively on OVV sites, while the second hydrogen abstraction can also occur on V−O−Si bridging oxygen sites.
Bestrijding van turkse mot met een nieuw baculovirus (2005)

De werkzaamheid van het baculovirus varieert nogal binnen de getoetste gewassen. Om hiervan een beter beeld te krijgen vindt een bredere screening plaats in samenwerking met een tweetal toeleverende producenten van baculovirussen.
Effect of ninjin'yoeito, a <scp>K</scp>ampo (traditional <scp>J</scp>apanese) medicine, on cognitive impairment and depression in patients with <scp>A</scp>lzheimer's disease: 2 years of observation (2015)

A 2-year follow-up of patients receiving donepezil and NYT treatment showed an improved cognitive outcome and alleviation of AD-related depression.
P1atinum:Platinum-Rhodium Thermocouple Wire IMPROVED THERMAL STABILITY ON YTIRlUM ADDITION TO PLATINUM (1997)

A new type of p1atinum:platinum-rhodium thermocouple wire which incorporates traces ofyttrium in the platinum limb has been developed and tested in some typical working environments. This thermocouple possesses good thermal stability and mechanical strength at high temperatures, and a long service life, compared with conventional p1atinum:platinum-rhodium thermocouples. The thermocouple meets the output requirements of the Type S standard for thermocouples - those made of Pt:Pt-lO%Rh - whose manufacturing tolerances are prescribed by the International Electrotechnical Commission (I. E.C.) (I). The life of thermocouples made from this wire is increased by around 1.5 to 2 times and they display a greater resistance to contamination. Large amounts of p1atinum:platinum-rhodium (Pt:Pt-Rh) thermocouple wire are used each year in China, for monitoring temperature in the iron and steel industries, during melting, annealing and metal forming processes. Other industries also utilise these materials. Platinum, however, is costly (around $380/troy ounces in March 1997) and as China produces only a small amount of platinum each year, with about 90 per cent of the total requirement being imported, there is a need for a less costly device. Pt:Pt-Rh thermocouples are normally used in temperature regions above 1000°C. For instance thermocouples in the glass industry are used at temperatures of 1000 to 1200°C and last for 5 to 7 years. At these temperatures the grain size of the platinum negative limb will increase; creep will occur and the material will become weak. When the temperature reaches about 1400°C, continuous oxidation will occur on the surface of the platinum wire, causing it to increase in size at a rate of approximately 39 x lo-’ mg cm-* h-I. The physical properties of platinum and platinum-rhodium are different; for example, at 1200°C the tensile strength for the pure platinum negative limb is 3.9 MPa, but it is 11 MPa for the platinum-10 per cent rhodium alloy positive limb
Facelet-Bank for Fast Portrait Manipulation (2018)

Digital face manipulation has become a popular and fascinating way to touch images with the prevalence of smartphones and social networks. With a wide variety of user preferences, facial expressions, and accessories, a general and flexible model is necessary to accommodate different types of facial editing. In this paper, we propose a model to achieve this goal based on an end-to-end convolutional neural network that supports fast inference, edit-effect control, and quick partial-model update. In addition, this model learns from unpaired image sets with different attributes. Experimental results show that our framework can handle a wide range of expressions, accessories, and makeup effects. It produces high-resolution and high-quality results in fast speed.
Alzheimer-like phosphorylation of tau and neurofilament induced by cocaine in vivo. (2003)

Peritoneal injection of cocaine induces Alzheimer-like hyperphosphorylation of tau and neurofilament in rat brain, and the effect may be not relevant to an increase in overexpression or overactivation of CDK5.