gebhart/patrepeval huggingface dataset:
Perspectives on the metabolic management of epilepsy through dietary reduction of glucose and elevation of ketone bodies (2003)

Brain cells are metabolically flexible because they can derive energy from both glucose and ketone bodies (acetoacetate and beta-hydroxybutyrate). Metabolic control theory applies principles of bioenergetics and genome flexibility to the management of complex phenotypic traits. Epilepsy is a complex brain disorder involving excessive, synchronous, abnormal electrical firing patterns of neurons. We propose that many epilepsies with varied etiologies may ultimately involve disruptions of brain energy homeostasis and are potentially manageable through principles of metabolic control theory. This control involves moderate shifts in the availability of brain energy metabolites (glucose and ketone bodies) that alter energy metabolism through glycolysis and the tricarboxylic acid cycle, respectively. These shifts produce adjustments in gene-linked metabolic networks that manage or control the seizure disorder despite the continued presence of the inherited or acquired factors responsible for the epilepsy. This hypothesis is supported by information on the management of seizures with diets including fasting, the ketogenic diet and caloric restriction. A better understanding of the compensatory genetic and neurochemical networks of brain energy metabolism may produce novel antiepileptic therapies that are more effective and biologically friendly than those currently available.
EXTRACTING FACES AND FACIAL FEATURES FROM COLOR IMAGES (2008)

In this paper, we present image processing and pattern recognition techniques to extract human faces and facial features from color images. First, we segment a color image into skin and non-skin regions by a Gaussian skin-color model. Then, we apply mathematical morphology and region filling techniques for noise removal and hole filling. We determine whether a skin region is a face candidate by its size and shape. Principle component analysis (PCA) is used to verify face candidates. We create an ellipse model to locate eyes and mouths areas roughly, and apply the support vector machine (SVM) to classify them. Finally, we develop knowledge rules to verify eyes. Experimental results show that our algorithm achieves the accuracy rate of 96.7% in face detection and 90.0% in facial feature extraction.
Metformin kills and radiosensitizes cancer cells and preferentially kills cancer stem cells (2012)

Metformin kills and radiosensitizes cancer cells and eradicates radioresistant cancer stem cells by activating AMPK and suppressing mTOR.
Carbonic Anhydrase Regulate Endometrial Gland Development in the Neonatal Uterus1 (2005)

Carbonic anhydrases (CAs) are zinc metalloenyzmes that catalyze the reversible conversion of carbon dioxide to carbonic acid and are involved in respiration, calcification, acid-base balance, and formation of fluids. Transcriptional profiling of the developing neonatal mouse uterus detected expression of Car1, Car2, Car11, and Car13 between Postnatal Days (PNDs) 3 and 18. In the neonatal mouse uterus, Car2 and Car11 mRNAs were predominantly localized in endometrial epithelial and stromal cells, respectively, whereas Car13 mRNA was detected in both epithelia and stroma. CAR2 protein was detected primarily in the endometrial epithelia and from PND 3 to PND 18 in the uteri of neonatal mice. To determine whether CA regulated uterine development, neonatal mice were treated s.c. with acetazolamide, a CA inhibitor, from PND 3 to PND 18. Treatment with acetazolamide decreased CA activity in the uterus and the number of endometrial glands without apparent effects on differentiation of the stroma or myometrium. In the neonatal sheep uterus, CA2 mRNA was initially expressed at birth (PND 0) in the endometrial luminal epithelium and was predominantly expressed in the developing glandular epithelium from PND 7 to PND 56. These results support the hypothesis that CA has a functional role in endometrial gland development during postnatal uterine morphogenesis.
The Type B Phosphatidylinositol-4-Phosphate 5-Kinase 3 Is Essential for Root Hair Formation in<i>Arabidopsis thaliana</i> (2008)

Root hairs are extensions of root epidermal cells and a model system for directional tip growth of plant cells. A previously uncharacterized Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase gene (PIP5K3) was identified and found to be expressed in the root cortex, epidermal cells, and root hairs. Recombinant PIP5K3 protein was catalytically active and converted phosphatidylinositol-4-phosphate to phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2]. Arabidopsis mutant plants homozygous for T-DNA-disrupted PIP5K3 alleles were compromised in root hair formation, a phenotype complemented by expression of wild-type PIP5K3 cDNA under the control of a 1500-bp PIP5K3 promoter fragment. Root hair-specific PIP5K3 overexpression resulted in root hair deformation and loss of cell polarity with increasing accumulation of PIP5K3 transcript. Using reestablishment of root hair formation in T-DNA mutants as a bioassay for physiological functionality of engineered PIP5K3 variants, catalytic activity was found to be essential for physiological function, indicating that PtdIns(4,5)P2 formation is required for root hair development. An N-terminal domain containing membrane occupation and recognition nexus repeats, which is not required for catalytic activity, was found to be essential for the establishment of root hair growth. Fluorescence-tagged PIP5K3 localized to the periphery of the apical region of root hair cells, possibly associating with the plasma membrane and/or exocytotic vesicles. Transient heterologous expression of full-length PIP5K3 in tobacco (Nicotiana tabacum) pollen tubes increased plasma membrane association of a PtdIns(4,5)P2-specific reporter in these tip-growing cells. The data demonstrate that root hair development requires PIP5K3-dependent PtdIns(4,5)P2 production in the apical region of root hair cells.
The encephalomyocarditis virus (2012)

The encephalomyocarditis virus (EMCV) is a small non-enveloped single-strand RNA virus, the causative agent of not only myocarditis and encephalitis, but also neurological diseases, reproductive disorders and diabetes in many mammalian species. EMCV pathogenesis appears to be viral strain- and host-specific, and a better understanding of EMCV virulence factors is increasingly required. Indeed, EMCV is often used as a model for diabetes and viral myocarditis, and is also widely used in immunology as a double-stranded RNA stimulus in the study of Toll-like as well as cytosolic receptors. However, EMCV virulence and properties have often been neglected. Moreover, EMCV is able to infect humans albeit with a low morbidity. Progress on xenografts, such as pig heart transplantation in humans, has raised safety concerns that need to be explored. In this review we will highlight the biology of EMCV and all known and potential virulence factors.
Tension-free mesh hernia repair: recovery and recurrence after one year. (1999)

The outcome of 200 consecutive patients with inguinal hernias repaired by the tension-free mesh technique at a specialist clinic was assessed one year after operation. There were no recurrences and no major complications. The wound infection rate was 1%. Less than 10% of patients had only minor complaints and 99% of patients were satisfied or very satisfied with the operation and its outcome. Low recurrence rates after one year imply low long-term recurrence and testify to the benefits of a specialist approach.
Role of interferon in melanoma: old hopes and new perspectives (2017)

Interferons (IFNs) play a key role in modulating anti-microbial and antitumor immune responses. In oncology, past attempts to exploit IFNs therapeutically did not fulfill expectations, and had only modest clinical results, mostly limited to adjuvant melanoma treatment. The recent successes of immunotherapy in oncology have brought new attention to the potential of immune-modulatory agents like the IFNs. Areas covered: The authors review the biological effects of IFN on melanoma and immune cells. Then, the authors summarize the clinical results of adjuvant and therapeutic IFN in melanoma, giving focus to possible prognostic factors and new on-going clinical trials. Expert opinion: IFNs offer intriguing opportunities for synergism between conventional treatments and recently introduced molecular-targeted and immunotherapy approaches. However, the full comprehension of all IFN effects and their multiple biologic links is challenging. A strong commitment toward parallel translational research is needed to facilitate the interpretation of IFN's expected and unexpected effects, guiding the rational design of informative clinical studies.
Astigmatism-corrected echelle spectrometer using an off-the-shelf cylindrical lens (2017)

As a special kind of spectrometer with the Czerny-Turner structure, the echelle spectrometer features two-dimensional dispersion, which leads to a complex astigmatic condition. In this work, we propose an optical design of astigmatism-corrected echelle spectrometer using an off-the-shelf cylindrical lens. The mathematical model considering astigmatism introduced by the off-axis mirrors, the echelle grating, and the prism is established. Our solution features simplified calculation and low-cost construction, which is capable of overall compensation of the astigmatism in a wide spectral range (200-600 nm). An optical simulation utilizing ZEMAX software, astigmatism assessment based on Zernike polynomials, and an instrument experiment is implemented to validate the effect of astigmatism correction. The results demonstrated that astigmatism of the echelle spectrometer was corrected to a large extent, and high spectral resolution better than 0.1 nm was achieved.
Photonic crystal Fano structures and their application to ultrafast switching and lasers (2016)

We present investigations on photonic-crystal Fano structures based on a cavity-waveguide configuration. We show that the use of Fano resonance can enable great improvements in high-speed low-energy all-optical switching and realizing ultra-fast nanolasers.
Modified Adenoviral Vectors Ablated for Coxsackievirus–Adenovirus Receptor, <i>α</i> v Integrin, and Heparan Sulfate Binding Reduce <i>In Vivo</i> Tissue Transduction and Toxicity (2006)

Coxsackievirus and adenovirus receptor (CAR), alphav integrins, and heparan sulfate glycosaminoglycans (HSGs) are the tropism determinants of adenoviral (Ad) vectors in vivo. For the development of a targeted Ad vector, its broad tropism needs to be blocked (or reduced). We have previously developed Ad vectors with ablation of CAR, alphav integrin, and HSG binding by mutation of the FG loop in the fiber knob (deletion of T489, A490, Y491, and T492 of the fiber protein), deletion of the RGD motif of the penton base, and substitution of the fiber shaft domain for that derived from Ad type 35, respectively, and have shown that this triple-mutant Ad vector [Ad/deltaF(FG)deltaP-S35-L2] exhibits significantly lower transduction in mouse liver compared with the conventional Ad vector [Koizumi, N., Mizuguchi, H., Sakurai, F., Yamaguchi, T., Watanabe, Y., and Hayakawa, T. (2003). J. Virol. 77, 13062-13072]. In the present study, we optimized the fiber knob mutation for further reduced in vivo transduction and examined toxicity of the modified Ad vectors. Ad/deltaF(AB)deltaPS35- L2, a triple-mutant Ad vector containing a mutation of the AB loop in the fiber knob (R412S, A415G, E416G, and K417G), mediated approximately 15,000- and 500-fold lower mouse liver transduction by intravenous and intraperitoneal administration, respectively, than the conventional Ad vector, and mediated 10- fold lower mouse liver transduction than did Ad/deltaF(FG)deltaP-S35-L2. Ad/deltaF(AB)deltaP-S35-L2 also exhibited lower transduction of other organs compared with Ad/deltaF(FG)deltaP-S35-L2 and the conventional Ad vector. Levels of both liver serum enzymes (aspartate transferase [AST] and alanine transferase (ALT)] and interleukin (IL)-6 in mouse serum after intravenous administration of Ad/deltaF(AB)deltaP-S35-L2 were similar to those in the nontreatment mouse serum, whereas the conventional Ad vector led to high levels of AST, ALT, and IL-6. We therefore succeeded in further improving the mutant Ad vector, abolishing both viral natural tropism and toxicity. This new Ad vector appears to be a fundamental vector for targeted gene delivery.
Relevance of extracellular matrix and its receptors in mammalian nephrogenesis revealed by metanephric organ culture system (2002)

Mammalian nephrogenesis is modulated by a number of extracellular matrix (ECM) glycoproteins, integrins and cell adhesion molecules. We demonstrated the existence of integrins alphavbeta1, alphavbeta3, alphavbeta5 and alphavbeta6 in epithelial elements of developing nephrons. Fibrillin-1 is a putative ligand for integrin alphavbeta3, and tubulointerstitial nephritis antigen (TIN-ag) is a ligand for integrins alphavbeta3 and alpha3beta1. Fibrillin-1 and TIN-ag are also differentially expressed in the developing kidney. The inclusion of antisense oligonucleotide in a mouse kidney organ culture system indicated that the alphav-related integrins and their ligands play an important role in mammalian nephrogenesis. Recently identified modulators of cell-matrix interactions, i.e. beta-galactoside-binding mammalian lectins (galectins), are involved in cell-cell and cell-matrix interactions by cross-linking glycoconjugates located on the ECM and membrane-bound glycoproteins. We identified and cloned a new member of the galectins from embryonic kidneys, and designated it galectin-9. Since high glucose alters the expression of ECM proteins and integrins, we also investigated the influence of glucose on metanephric development. The presence of 30 mM D-glucose in metanephric organ culture induced dysmorphogenesis of the kidney accompanied by decreased expression of perlecan. Furthermore, we screened the genes differentially expressed under high glucose conditions in streptozotocin-induced newborn mouse kidneys by representational difference analysis of cDNA. We identified translocase of inner mitochondrial membrane (Tim44) and renal-specific oxido-reductase (RSOR). The roles of these molecules in glucose-induced dysmorphogenesis and the relationship with ECM-related molecules need to be addressed.
Alcohol Inhibition of Cell Adhesion in BMP‐Treated NG108‐15 Cells (1999)

These data suggest that ethanol inhibits cell-cell adhesion in OP-1-treated NG108-15 cells by interacting directly or indirectly with the neuronal isoform of L1.
Increased Survival and Vascularity of Random-Pattern Skin Flaps Elevated in Controlled, Expanded Skin (1983)

Controlled clinical tissue expansion, a new technique of providing donor tissue, results in an increase in surface area of expanded skin. The aim of the present study was to determine the effect of controlled tissue expansion on the surviving lengths of random-pattern skin flaps elevated in expanded tissue. In five pigs the surviving lengths of flaps raised in skin expanded for 5 weeks using a 250-cc rectangular Radovan-type tissue expander were compared with the survival lengths of flaps elevated in tissue in which a similar prosthesis was not expanded, bipedicle flaps delayed for 5 weeks, and control acutely raised random-pattern flaps. The expanded flaps had a mean increase in surviving length of 117 percent over control flaps, which was statistically significant. The delay flaps had an increase in survival of 73 percent over control flaps, which was also statistically significant. There was no significant difference in survival between expanded flaps and delayed flaps. Morphologic studies using radiographic techniques on one pig demonstrated increased vascularity with tissue expansion. The results of this work demonstrate that in addition to providing increased surface area with controlled expansion, flaps raised in expanded skin have a significantly augmented surviving length. The mechanism for this increased vascularity with expansion is not known at this time, but it may be due to physical forces associated with expansion acting as a stimulus for angiogenesis.
Integration of flywheel-based energy storage for frequency regulation in deregulated markets (2010)

Flywheel-based energy storage is being introduced on a large scale (20 MW) for providing grid frequency regulation in deregulated markets. The ISOs have already introduced, or are in the process of introducing, market rules and tariffs to comply with FERC Order No. 890 to allow new competitive technologies to participate in the markets. Analysis of more than one year of operating data shows flywheel-based storage can provide superior cost, emissions, and operational performance. Availability of this new grid technology is particularly important since the rapid addition of variable-generation renewable resources to meet RPS standards is expected to increase the need for regulation.This paper presents performance data from more than one year of operation in an ISO New England (ISO-NE) field trial, describes NYISO and MISO strategies for using fast-response Limited Energy Storage Resources (LESR) for maximum effectiveness, and describes the ISO-NE regulation control methodology that has achieved the lowest (best-in-class) amount of required regulation of any large grid in North America. Charge and discharge cycle requirements for energy storagebased technologies performing regulation are also extrapolated based on real operating data, and a practical definition of overall system efficiency is introduced. Lastly, status on the progress of the first two 20 MW flywheelbased regulation plants in NYISO and PJM and their related DOE funding support are presented.
Phosphodiesterase-4 inhibitors as a novel approach for the treatment of respiratory disease: cilomilast (2006)

Phosphodiesterase-4 (PDE4) is an important cAMP-metabolising enzyme in immune and inflammatory cells, airway smooth muscle and pulmonary nerves. The phosphodiesterase 4 (PDE4) enzyme plays a significant role in modulating the activity of cAMP, an important second messenger that mediates the relaxation of airway smooth muscle and suppresses inflammatory cell function, thereby attenuating the inflammatory response. Selective inhibitors of this enzyme show a broad spectrum of activity in animal models of COPD and asthma. These drugs block the hydrolysis of cAMP via inhibition of PDE4 and are attractive candidates for novel anti-inflammatory drugs. At present, two second-generation PDE4 inhibitors for the treatment of COPD and asthma patients are being tested in clinical Phase III trials. The most advanced compound is the orally active, selective PDE4 inhibitor cilomilast (Ariflo, SB-207499, cis-4-cyano-4-[3-cyclopentyloxy-4-methoxyphenyl]-cyclohexanecarboxylic acid; GlaxoSmithKline). Cilomilast shows high selectivity for cAMP-specific PDE4, an isoenzyme that predominates in pro-inflammatory and immune cells and that is 10-fold more selective for PDE4D than for PDE4A, -B or -C. In vitro, cilomilast suppresses the activity of several pro-inflammatory and immune cells that have been implicated in the pathogenesis of asthma and COPD. Moreover, it is highly active in animal models of these diseases. Cilomilast has been shown to exert potent anti-inflammatory effects both in vitro and in vivo. It is orally active and may be effective in the treatment of asthma and COPD; however, complete assessment of the therapeutic value of this novel compound class must await the outcome of longer-term clinical trials. This review presents a summary of the preclinical and clinical profile of cilomilast in patients with COPD.
Applying a Model Transformation Taxonomy to Graph Transformation Technology (2006)

A taxonomy of model transformations was introduced in [T. Mens, P.V. Gorp, A taxonomy of model transformation, in: Proc. Int'l Workshop on Graph and Model Transformation (GraMoT 2005), Electronic Notes in Computer Science (2005)]. Among others, such a taxonomy can help developers in deciding which language, forma lism, tool or mechanism is best suited to carry out a particular model transformation activity. In this paper we apply the taxonomy to the technique of graph transformation, and we exemplify it by referring to four representative graph transformation tools. As a byproduct of our analysis, we discuss how well each of the considered tools carry out the activity of model transformation.
Identity elements of <i>Escherichia coli</i> tRNA<sup>Ala</sup> (1991)

Studies using the T7 transcription system revealed that the discriminator base A73 and the G20 in the variable pocket play important roles in the Escherichia coli alanine tRNA identity. The C60 in the T-loop, which is unique to alanine tRNA, was not found to be crucial for alanine identity. Anticodon replacement into the valine anticodon UAC did not decrease alanine charging activity, and no alanine charging activity was detected in the mutant valine tRNA possessing the alanine anticodon UGC.
S-Adenosylhomocysteine hydrolase from rat liver. Purification and some properties. (1981)

S-Adenosylhomocysteine hydrolase (EC 3.3.1.1) from rat liver was purified to homogeneity. The enzyme had a molecular weight of 188,000 and was composed of 4 subunits with a molecular weight of 47,000. The isoelectric pH of the enzyme was at 5.7. The Km values for S-adenosyl-L-homocysteine, adenosine, and DL-homocysteine were 15.2 microM, 1.05 microM, and 155 microM, respectively, at pH 7.2 and 25 degrees C. The enzyme showed a fluorescence with an emission maximum at 350 nm when excited at 280 nm. The protein fluorescence was partially quenched on addition of adenosine. The fluorescence quenching as a function of adenosine concentration indicated that the enzyme contained four binding sites for adenosine per molecule. The same data provided a value of 0.63 microM for the dissociation constant of adenosine. The enzyme possessed 4 mol of tightly bound NAD+/mol. The addition of adenosine to the enzyme caused the appearance of a peak at 327 nm in a concentration-dependent manner. The enzyme-bound NAD+ readily formed an adduct with bisulfite with the concomitant loss of enzyme activity. These data suggest that the bound NAD+ is involved in the catalytic mechanism of the enzyme.
Observation of Heat Diffusion across Submicrometer Metal Thin Films Using a Picosecond Thermoreflectance Technique (1999)

We have observed heat diffusion across submicrometer metal thin films for the first time using a picosecond thermoreflectance method. The boundary between a film and a transparent substrate is heated by a picosecond laser pulse. Heat generated by the pump laser pulse diffuses towards the front surface of the thin film. The temperature change on the front surface opposite to the heated area is probed by the reflectivity of another picosecond laser pulse. Thermoreflectance signals of a molybdenum thin film and an aluminum thin film with nominal thickness of 100 nm deposited on Pyrex 7740 glass substrates were observed at room temperature. Thermal energy transfer inside the molybdenum and aluminum thin films under picosecond heating can be explained by the classical heat diffusion equation. Thermal diffusivity values are close to those of bulk molybdenum and bulk aluminum, respectively.
A Systematic Review of Techniques for Test Case Prioritization (2013)

In software development life cycle, maintenance phase is an important phase as it deals with the activities like error correction, upgradation, deletion and optimization of software capabilities. For this reason, regression testing is required in order to revalidate the modifications in the software. It is an expensive process. Various techniques of performing regression testing are available. Software testers can select the technique that suit their requirement as well as optimize the basic cost and time factors. This paper mainly discusses various test case prioritization techniques for regression testing presented by various researchers and the various search algorithms used in the test case prioritisation process.