gebhart/patrepeval huggingface dataset:
Cloning and Expression of an HIV‐1 Specific Single‐Chain F<sub>v</sub> Region Fused to <i>Escherichia coli</i> Alkaline Phosphatase<sup>a</sup> (1991)

We have constructed a single-chain Fv fragment representing the variable domain of the human monoclonal antibody 3D6, binding specifically to HIV-1 gp41. This gene was fused to the coding region of E. coli alkaline phosphatase (EcPhoA) and expressed in E. coli. The EcPhoA signal peptide was used to direct the recombinant fusion protein to the periplasmic space of the bacteria, from where it was purified by hydrophobic interaction chromatography and gel filtration followed by antigen-affinity chromatography using a synthetic HIV-1 peptide as ligand. The purified fusion protein was bifunctional, showing both phosphatase activity as well as antigen-binding specificity identical to that of the original antibody.
4-Alkyl-5-aryl-4H-1,2,4-triazole-3-thiols as hypoglycemic agents (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT4-Alkyl-5-aryl-4H-1,2,4-triazole-3-thiols as hypoglycemic agentsM. Y. Mhasalkar, Madhusudan H. Shah, S. T. Nikam, Kodaganallur G. Anantanarayanan, and Chimanlal V. DeliwalaCite this: J. Med. Chem. 1970, 13, 4, 672–674Publication Date (Print):July 1, 1970Publication History Published online1 May 2002Published inissue 1 July 1970https://pubs.acs.org/doi/10.1021/jm00298a021https://doi.org/10.1021/jm00298a021research-articleACS PublicationsRequest reuse permissionsArticle Views152Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
<title>Two-way memory effect (TWME) in NiTi-Pd high-temperature shape memory alloys</title> (1994)

A preliminary investigation was made to study the possibility of training the newly developed TiNi-Pd high temperature shape memory alloys to posses the two-way memory effect, and characterize the various factors that affect the magnitude of the two-way memory effect. The experimental results show that all the newly developed alloys can demonstrate the two-way memory effect, even though the transformation temperature of the alloy reaches 280 degree(s)C. The permanent strain and phase transformation temperature are two important parameters to determine the magnitude of the two-way memory effect.
Protein synthesis is shutdown in dormant<i>Mycobacterium tuberculosis</i>and is reversed by oxygen or heat shock (1998)

Oxygen-limiting conditions are critical to the survival of the bacteria in tuberculosis. Mycobacterium tuberculosis can survive anaerobiosis in vitro for long periods of time only after a gradual transition to a microaerophilic stationary phase. The underlying mechanism behind stationary phase adaption needs to be elucidated. The protein profiles of Mycobacterium tuberculosis during long-term stationary phase growth and under strict anaerobic incubation were monitored by [35S]methionine labelling, SDS-PAGE and fluorography. These experiments have established that protein synthesis gradually decreased over 50 days in the long-term stationary phase cultures which were considered to be microaerophilic. There was an 80% linear decrease in the level of total protein synthesis during the first 40 days of microaerophilic growth and then the rate of protein synthesis faded quickly. For the first time we have shown that total protein synthesis shutdown occurred when bacilli were incubated under further anaerobic conditions. Viability, estimated by cfu counts, remained constant during stationary phase growth and under anaerobic incubation. Furthermore, when oxygen was introduced into the anaerobic culture, protein synthesis restarted. Also heat shock at 45 degrees C, 48 degrees C and 50 degrees C rapidly induced protein synthesis in stationary and anaerobic cultures. These data indicate that dormant bacteria shut down protein synthesis but remain responsive to specific stimuli which restore protein synthesis. In addition the dormant bacilli induced by anaerobiosis developed more heat resistance than nondormant organisms.
Potential Role of Creb As A Prognostic Marker In Acute Myeloid Leukemia (2007)

The cAMP response element binding protein (CREB) is a leucine zipper transcription factor that regulates genes responsible for cell proliferation, differentiation and survival. CREB is overexpressed in the bone marrow from most patients with acute leukemia. Overexpression of CREB occurs both at the protein and at the transcript levels and is associated with gene amplification in leukemic blast cells. Higher levels of CREB correlate with a less favorable prognosis in a small cohort of adult patients with acute myeloid leukemia. In one study, patients whose bone marrow over-expresses CREB had an increased risk of relapse and decreased event-free survival. Mice that overexpress CREB in myeloid cells develop a myeloproliferative/myelodysplastic syndrome. These findings suggest that CREB plays an important role in the pathogenesis of acute leukemia and is a potential biomarker of disease.
Photoluminescence enhancement in nanocomposite thin films of CdS–ZnO (2005)

We show that the photoluminescence emitted from a dense, two-component quantum dot ensemble on a thin film is significantly higher and decays much faster than that from quantum dots of either of the two pure systems (CdS and ZnO). The semiconductor nanocomposite, in which the characteristic grain size of each species was 2–3nm, was deposited directly on Si wafers by high-pressure magnetron sputtering, and exhibits a single, relatively sharp optical absorption edge.
Hot Wire Air Flow Meter for Engine Control Systems (1990)

<div class="htmlview paragraph">A new type hot wire probe has been developed for measurement of intake mass air flow rate in electronic fuel injection systems. The probe was composed of a coiled platinum wire covered with a glass-ceramic composite material. Improvement in response time has been achieved through the reduction of heat transfer from the hot wire probe to the leading wires, resulting from low thermal conductivity of the covering material. The decrease of the longitudinal heat flow rate has been revealed by the measurement of temperature distribution in the probe.</div>
Survivin is a shared tumor-associated antigen expressed in a broad variety of malignancies and recognized by specific cytotoxic T cells (2003)

Survivin, a member of the inhibitor of apoptosis protein family, is expressed in almost all types of malignancies, making this protein a useful tool for the development of broadly applicable vaccination therapies. We used a recently identified HLA-A2 binding peptide and dendritic cells (DCs) from healthy donors to induce survivin-specific cytotoxic T lymphocytes (CTLs) in vitro. These T cells efficiently lysed target cells pulsed with the cognate peptide. Furthermore, survivin-specific CTLs recognized HLA-A2-matched tumor cell lines and primary malignant cells from patients with leukemia in an antigen-specific and HLA-restricted manner as demonstrated with the use of cold target inhibition assays and blocking antibodies. To validate the immunogenicity of survivin we performed the experiments in an autologous setting and used monocyte-derived DCs as targets. Interestingly, we found that DCs up-regulate survivin expression on stimulation with tumor necrosis factor alpha (TNF-alpha). However, these mature DCs were not recognized by survivin-specific CTLs, whereas they lysed autologous mature DCs pulsed with the antigenic peptide or transfected with whole tumor RNA purified from a survivin-expressing cell line. To further analyze the possible use of survivin-specific CTLs in cancer therapies, we induced survivin-specific CTLs using peripheral blood mononuclear cells (PBMNCs) and DCs from a patient with chronic lymphocytic leukemia (CLL). The in vitro-generated T cells efficiently recognized autologous malignant CLL cells, whereas they spared autologous-purified nonmalignant B cells or DCs. Our results demonstrate that survivin epitopes are presented on a broad variety of malignancies and can be applied in vaccination therapies.
Digital control of dynamic systems (1980)

From the Publisher:
This well-respected, market-leading text discusses the use of digital computers in the real-time control of dynamic systems. The emphasis is on the design of digital controls that achieve good dynamic response and small errors while using signals that are sampled in time and quantized in amplitude. Both classical and modern control methods are described and applied to illustrative examples. The strengths and limitations of each method are explored to help the reader develop solid designs with the least effort. Two new chapters have been added to the third edition offering a review of feedback control systems and an overview of digital control systems. Updated to be fully compatible with MATLAB versions 4 and 5, the text thoroughly integrates MATLAB statements and problems to offer readers a complete design picture. The new edition contains up-to-date material on state-space design and twice as many end-of-chapter problems to give students more opportunities to practice the material.
CROSI Mapping System (CMS) - Result of the 2005 Ontology Alignment Contest. (2005)

In this results report we summarize our experiences from running the CROSI Mapping System (CMS) over three test cases for this year’s OAEI contest: bibliography, Web directories and medical ontologies alignment case studies. CMS successfully parsed and aligned all input ontologies in all three case studies. We also elaborate on the insights gained and potential research directions towards building more robust alignment systems to cope with the increasing diversity of alignment requirements.
Neurofibromin regulates corticostriatal inhibitory networks during working memory performance (2010)

Neurofibromatosis type I (NF1) is one of the most common single-gene causes of learning disabilities. Here, we use behavioral working memory probes and electrophysiological studies in a mouse model of NF1 ( Nf1 heterozygous null mutants; Nf1 +/− ) to demonstrate that ( i ) Neurofibromin regulates prefrontal and striatal inhibitory networks, specifically activity-dependent GABA release and ( ii ) is required for working memory performance, with inhibition-dependent working memory deficits seen in Nf1 +/− mice. We find that increased inhibition in medial prefrontal cortex (mPFC) is sufficient to alter persistent activity in a biophysical model of an mPFC microcircuit, suggesting a possible mechanism for Nf1 +/− working memory deficits. Accordingly, working memory assays applied during functional MRI (fMRI) studies in human subjects with NF1 reveal hypoactivation of corticostriatal networks, which is associated with impaired working memory performance. Collectively, these integrative mouse and human studies reveal molecular and cellular mechanisms contributing to working memory deficits in NF1.
Thermoformable Polyurethane Foam for the Manufacturing of Headliners and Other Automotive Interior Trim Parts (1988)

Utilisation de la mousse Elastoflex W. Composition chimique, proprietes physiques, mise en œuvre, proprietes des pieces finies
Structural Effects on a Submicron Trench Process (1985)

Recently, silicon trenches have been widely used for device isolation or three‐dimensional capacitors in dynamic memories. In this work, trenches with submicron openings have been fabricated and several structure‐dependent effects were discovered. A reactive ion etching rate in the vertical direction inside a trench decreases significantly as the aspect ratio (depth/width) becomes larger. A bottle‐shaped profile due to undercutting starts to appear with trenches whose openings are smaller than a micrometer and becomes worse as the width decreases. A film thickness on sidewalls for refilling trenches shows no strong dependence on the structural aspect when chemically vapor deposited at low pressure.
Observation of coherent Smith-Purcell radiation from short-bunched electrons (1995)

Coherent Smith-Purcell radiation, generated by the passage of short-bunched electrons of relativistic energy of 42 MeV above the surface of a metallic grating, has been observed in the wavelength region from 0.5 to 4.0 mm. The intensity of the radiation is proportional to the square of the beam current. The intensity at \ensuremath{\lambda}=2.5 mm is enhanced by several orders of magnitude compared with the incoherent radiation. It is found that the intensity decreases proportionally to the square of the modified Bessel function [${\mathit{K}}_{0}$(2\ensuremath{\pi}h/\ensuremath{\lambda}\ensuremath{\beta}\ensuremath{\gamma})${]}^{2}$, as the beam height h from the surface of the grating increases.
Interferon consensus sequence-binding protein, a member of the interferon regulatory factor family, suppresses interferon-induced gene transcription. (1993)

We previously isolated a cDNA clone encoding interferon consensus sequence-binding protein (ICSBP), a member of the interferon regulatory factor (IRF) family, that binds to the interferon (IFN)-stimulated response element (ISRE) of many IFN-regulated genes. In this investigation, we studied the functional role of ICSBP by transient cotransfection of ICSBP cDNA with IFN-responsive reporter genes into the human embryonal carcinoma cell line N-Tera2. These cells were shown not to express ICSBP or IRF-2, thus allowing functional analysis of transfected cDNAs. Cotransfection of ICSBP into cells treated with retinoic acid or any of the IFNs (alpha, beta, or gamma) repressed expression of a chloramphenicol acetyltransferase reporter driven by the major histocompatibility complex class I gene promoter. Similarly, ICSBP repressed expression of chloramphenicol acetyltransferase reporters driven by the ISREs of the 2'-5' oligoadenylate synthetase, guanylate-binding protein, and ISG-15 genes in IFN-treated cells. The repression was dependent on the presence of the ISRE in the reporter. Deletion analysis showed that the putative N-terminal DNA binding domain of ICSBP by itself is capable of mediating the repression. Using the same cotransfection conditions as for ICSBP, a similar repression of these reporters was observed with IRF-2. Finally, ICSBP repressed the IRF-1-mediated induction of major histocompatibility complex class I and IFN-beta reporters in the absence of IFN or retinoic acid. Taken together, these results suggest that ICSBP is a negative regulatory factor capable of repressing transcription of target genes induced by IFN, retinoic acid, or IRF-1.
XeCl Excimer Laser Annealing Used to Fabricate Poly-Si TFT's (1989)

Polycrystalline silicon thin-film transistors (poly-Si TFT's) with a high carrier mobility were fabricated at low processing temperatures of 150 and 250°C. A hydrogenated amorphous silicon (a-Si:H) film was successfully crystallized at room temperature by multistep irradiation of XeCl-308 nm excimer laser pulses without explosive evaporation of hydrogen. The poly-Si TFT's fabricated by the 250°C process had a carrier mobility of 54 cm 2 /V·s and a low potential barrier height at a grain boundary of 0.01 eV.
Procedures for setting curve advisory speed. (2009)

The objectives of this document and presentation are to (1) inform the audience about challenges associated with curve signing and the availability of tools to determine appropriate signs; and (2) demonstrate how to apply these tools.
A new procedure for classifying speakers in speaker verification systems (1997)

\n Contains fulltext :\n 74994.pdf (author's version ) (Open Access)\n
The evolution of plastic antibodies (2010)

Plastic antibodies, synthetic polymer nanoparticles with antibody-like functions, have emerged as potential alternatives to protein antibodies. This paper focuses on recent developments of plastic antibodies for biomacromolecules and their application as antitoxins.
Mechanism of plasma-induced damage to low-k SiOCH films during plasma ashing of organic resists (2011)

Plasma-induced damage to porous SiOCH (p-SiOCH) films during organic resist film ashing using dual-frequency capacitively coupled O2 plasmas was investigated using the pallet for plasma evaluation method developed by our group. The damage was characterized by ellipsometry and Fourier-transform infrared spectroscopy. Individual and synergetic damage associated with vacuum ultraviolet (VUV) and UV radiation, radicals, and ions in the O2 plasma were clarified. It was found that the damage was caused not only by radicals but also by synergetic reactions of radicals with VUV and UV radiation emitted by the plasmas. It is noteworthy that the damage induced by plasma exposure without ion bombardment was larger than the damage with ion bombardment. These results differed from those obtained using an H2/N2 plasma for resist ashing. Finally, the mechanism of damage to p-SiOCH caused by O2 and H2/N2 plasma ashing of organic resist films is discussed. These results are very important in understanding the mechanism of plasma-induced damage to p-SiOCH films.
A document classification and extraction system with learning ability (1999)

Document image processing begins at the OCR phase with the difficulty of automatic document analysis and understanding. Most existing systems only do well in their specific application domains. In this paper, we describe a domain-independent automatic document image understanding system with learning ability. A segmentation method based on "logical closeness" is proposed. A novel and natural representation of document layout structure-a directed weight graph (DWG)-is described. To classify a given document, a string representation matching algorithm is applied first, instead of comparing all the sample graphs. A frame template and a document type hierarchy (DTH) are used to represent the document's logical structure and the hierarchical relationships among these frame templates, respectively. In this paper, two learning methodologies are applied-learning from experience and an enhanced perceptron learning algorithm.
Increased expression of TIGIT on CD4<sup>+</sup>T cells ameliorates immune-mediated bone marrow failure of aplastic anemia (2014)

Aplastic anemia (AA) is an autoimmune disease in which T cell activation is suspected to play an important role. T cell immunoglobulin and ITIM (immunoreceptor tyrosine-based inhibition motif) domain (TIGIT) is an inhibitory receptor, which exhibits inhibitory functions on the immune response. However, its role in AA has not been clearly determined. In the current study, we showed that the frequency of TIGIT-positive CD4(+) T cells was reduced in the vast majority of AA patients (85%, 17/20). In TIGIT-silenced human CD4(+) T cells, stimulation of agonistic anti-TIGIT monoclonal antibody significantly facilitated cell proliferation, increased production of IL-2 and IFN-γ, and inhibited production of IL-10. However, in TIGIT-overexpressed human CD4(+) T cells, cell proliferation and the production of IL-2, IFN-γ, and TNF-α were significantly hindered; in contrast, the secretion of IL-10 was improved. RT-PCR and Western blotting showed that T-bet expression in human CD4(+) T cells was significantly decreased by TIGIT overexpression, but only slightly altered by TIGIT knockdown. In mouse models, lentivirus-mediated TIGIT-overexpressed CD4(+) T cell transfer significantly rescued the decreased red blood cell count, attenuated the increase in serum INF-γ and TNF-α levels, and lengthened the median survival time. The mRNA levels of CD34, stem cell factor (SCF), and granulocyte/macrophage-colony-stimulating factor (GM-CSF) in bone marrow mononuclear cells were also up-regulated. In conclusion, increased expression of TIGIT could inhibit the function of CD4(+) T cells in vitro and ameliorate immune-mediated bone marrow failure of AA in vivo providing a new potential strategy for the treatment of AA.
Pulsed multiwavelength laser ranging system (1982)

A pulsed multiwavelength laser ranging system for measuring atmospheric delay was built and tested, and its theoretical performance limits were calculated. The system uses a dye modelocked ND:YAG laser, which transmits 70 psec wide pulses simultaneously at 1064, 532, and 355 nm. The differential delay of the 1064 and 355 nm pulses is measured by a specially calibrated waveform digitizer to estimate the dry atmospheric delay. The delay time of the 532 nm pulse is used to measure the target distance. Static crossed field photomultipliers are used as detectors for all wavelengths. Theoretical analysis shows that path curvature and atmospheric turbulence are fundamental limits to the ranging accuracy of both single and multicolor systems operating over horizontal paths. For two color systems, an additional error is caused by the uncertainty in the path averaged water vapor. The standard deviation of the multicolor instrument's timing measurements is directly proportional to the laser pulse width plus photomultiplier jitter divided by the square root of the received photoelectron number. The prototype system's maximum range is km, which is limited by atmospheric and system transmission losses at 355 nm. System signal detection and false alarm calculations are also presented.
LDPC Codes in Communications and Broadcasting (2007)

Low-density parity-check (LDPC) codes are one of the most powerful error correcting codes and are attracting much attention these days.LDPC codes are promising for communications and broadcasting as well where the use of error correcting codes are essential.LDPC codes have been standardized in some communication standards, such as, IEEE802.16e,DVB-S2, IEEE802.3an(10BASE-T), and so on.The performance of LDPC codes largely depend on their code structure and decoding algorithm.In this paper, we present the basics of LDPC codes and their decoding algorithms.We also present some LDPC codes that have good performance and are receiving much attention particularly in communication systems.We also overview some standardized LDPC codes, the LDPC codes standardized in DVB-S2 and the IEEE802.16estandard LDPC codes.Moreover, we present some research on LDPC coded MIMO systems and HARQ using LDPC codes.