gebhart/patrepeval huggingface dataset:
Acridine Adduct of [60]Fullerene with Enhanced DNA-Cleaving Activity (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTAcridine Adduct of [60]Fullerene with Enhanced DNA-Cleaving ActivityYoko Nakajima Yamakoshi, Takeshi Yagami, Shoko Sueyoshi, and Naoki MiyataView Author Information Division of Organic Chemistry and Division of Medical Devices, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo 158, Japan Cite this: J. Org. Chem. 1996, 61, 21, 7236–7237Publication Date (Web):October 18, 1996Publication History Received27 June 1996Published online18 October 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/jo961210qhttps://doi.org/10.1021/jo961210qrapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views573Altmetric-Citations106LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Adducts,Cytosine,Electromagnetic radiation,Genetics,Irradiation Get e-Alerts
Dielectric characterization of transparent epitaxial Ga2O3 thin film on n-GaN∕Al2O3 prepared by pulsed laser deposition (2006)

( 2 ¯ 01 ) -oriented β-Ga2O3∕GaN thin films were epitaxially grown by pulsed laser deposition. These films have the specific in-plane orientation, which was confirmed by φ scans of Ga2O3 (111) and (3¯11) reflections. When oxygen flow rate was increased, the surface morphologies and roughness of β-Ga2O3 drastically changed. The β-Ga2O3∕GaN structure showed a stable and sharp interface and uniform elemental distribution in depth. The dielectric constant and memory window of β-Ga2O3∕GaN were about 13.9 and 0.50V for oxygen flow rate of 5SCCM (SCCM denotes cubic centimeter per minute at STP).
Further evidence that pathologic high-frequency oscillations are bursts of population spikes derived from recordings of identified cells in dentate gyrus (2011)

Pilocarpine-treated epileptic mice were anesthetized with urethane and ketamine. Their heads were fixed in a stereotaxic frame. Extracellular unit activity was recoded with glass micropipettes, whereas multiunit and local field activity was simultaneously recorded with attached tungsten microelectrodes. After electrophysiologic experiments, recorded cells were labeled by neurobiotin and visualized by immunohistochemical methods. KEY FINDINGS AND SIGNIFICANCES: pHFOs containing more than three waves were recorded in our experiments, but pathologic single-population spikes also occurred. Identified granular cells discharged preferentially in synchrony with pHFOs and single population spikes, whereas interneurons decreased their discharge frequency during this time. These experiments provide additional confirmation that pHFOs in the dentate gyrus represent single or recurrent population spikes, which in turn reflect summated hypersynchronous discharges of principal cells.
Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA (1998)

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.
Atomistic model of the spider silk nanostructure (2010)

Spider silk is an ultrastrong and extensible self-assembling biopolymer that outperforms the mechanical characteristics of many synthetic materials including steel. Here we report atomic-level structures that represent aggregates of MaSp1 proteins from the N. Clavipes silk sequence based on a bottom-up computational approach using replica exchange molecular dynamics. We discover that poly-alanine regions predominantly form distinct and orderly beta-sheet crystal domains while disorderly structures are formed by poly-glycine repeats, resembling 31-helices. These could be the molecular source of the large semicrystalline fraction observed in silks, and also form the basis of the so-called “prestretched” molecular configuration. Our structures are validated against experimental data based on dihedral angle pair calculations presented in Ramachandran plots, alpha-carbon atomic distances, as well as secondary structure content.
Strain-induced lateral carrier confinement in quantum wells grafted onto nonplanar substrates (1992)

We remove a thin semiconductor film from its growth substrate and reattach it to a nonplanar host substrate. The film is under a large, localized bending stress. In a GaAs/AlGaAs film with a quantum well near one surface where the bending strain is greatest, carriers are laterally confined by the strain to regions where the band gap is red-shifted by up to 62 meV.
Low-level methicillin resistance in strains of Staphylococcus aureus (1989)

Two strains of Staphylococcus aureus expressing borderline or low-level methicillin resistance by one or more in vitro test methods were examined for resistance in vivo and for biochemical and genetic markers of methicillin resistance. In vivo, nafcillin was equally effective against experimental aortic valve endocarditis in rabbits, regardless of whether they were infected by a fully susceptible or a low-level-resistant strain. Resistance did not emerge during therapy. For the more resistant of the two low-level-resistant strains, methicillin was as effective as nafcillin. Nafcillin was ineffective against endocarditis caused by a truly methicillin-resistant strain, and resistance emerged on therapy. The low-level-resistant strains did not produce the low-affinity penicillin-binding protein 2a that is associated with methicillin resistance and did not contain DNA that hybridized with probes that recognized the methicillin resistance determinant. Low-level resistance in S. aureus is a phenomenon that is biochemically and genetically distinct from true methicillin resistance. These strains actually are susceptible to beta-lactam antibiotics. The clinical problem posed by these strains is not a therapeutic one but, instead, one of how to differentiate them from those that are truly methicillin resistant.
Experimental Investigation of a Circular-Planform Concept Aircraft (2010)

This paper presents experimental results from wind-tunnel testing of a novel aircraft configuration known as the Geobat. This configuration has a circular planform with the forward portion of the disk serving as the main wing and body of the aircraft, and an aft portion that serves as the horizontal stabilizer. The two parts are attached at the wing tips much like a joined-wing design, and also near the centerline by two booms supporting vertical stabilizers. The center portion of the body is open. Wind-tunnel tests were conducted to determine the lift, drag, and pitching moment characteristics oftheconfiguration. For comparison purposes, two flat disks were also tested, one with a similar interior cutout and one solid across the full disk. Experimental data have been compared with vortex-lattice analysis for the solid and cutout shapes. Comparisons of the theory and experimental data indicate that viscous and vortex lift effects strongly influence the performance of the configuration, particularly at higher angles of attack. Both the experimental data and analysis show that the Geobat planform moves the aerodynamic center slightly aft, and the geometry tends to push the center of gravity forward, improving the static stability versus a solid-disk-shaped wing design.
Antioxidative activities of some chemotherapeutics. A possible mechanism in reducing gingival inflammation (1994)

Inflammatory periodontal diseases are related to dental plaque formation. Increase in the perfusion of the inflamed tissue results in increased oxygen supply. Although oxygen has healing effects, it is bound to be a mediator of peroxidation in biological membranes. Chemotherapeutic agents such as chlorhexidine, listerine, sanguinarine, and cetylpridinium chloride and oral antibiotics such as tetracycline HCl and doxycyline were tested for their antioxidative activities. While doxycycline has the highest antioxidant activity in lower volumes (0.1 ml), sanguinarine, listerine and a pace after them, tetracycline HCl, had similar effects in higher volumes (0.3 and 0.4 ml). The results showed that in addition to their antiseptic or antimicrobial effects, these preparations have an antioxidative activity against spontaneous oxidation.
First-Moment Analysis of Polarized Light Emission from InGaN/GaN Light-Emitting Diodes Prepared on Semipolar Planes (2006)

A first-moment analysis has been applied to electroluminescent spectra of polarized light from InGaN/GaN light-emitting diodes prepared on (1011) and (1013) planes. The normalized first moment (the first moment divided by the zeroth moment) of emission spectra clearly fluctuated due to polarizer rotational angle, even though the spectral peak shift was not obvious. A δ-function model of emission spectra, where two δ-functions represented two polarization components of the electroluminescence, was proposed to explain the observed fluctuation. This was reproduced very well by the model, and fitting parameters appeared to be reasonable numerical values.
Vasculogenic mimicry and pseudo‐comedo formation in breast cancer (2002)

Tumors require a blood supply for growth and hematogenous metastases. Until recently, most research in this area has focused on the role of angiogenesis, the recruitment of new vessels into a tumor from preexisting vessels. Previously, in a study of breast cancer (IBC), in which we used established inflammatory breast cancer (IBC) xenografts (WIBC-9) originating from a patient with IBC (Shirakawa et al., Cancer Res 2001:61:445-451), we reported observing vasculogenic mimicry (VM), a condition in which bloodstreams within cancer tissue are not accompanied by a lining of endothelial cells (ECs) (Shirakawa et al., Cancer Res 2002:62:560-566). In the present study, we examined 331 surgically resected breast cancer specimens for evidence of VM, using immunohistochemistry and laser-captured microdissection (LCM) followed by nested reverse transcriptase polymerase chain reaction (RT-PCR). Surprisingly, 7.9% (26 specimens) of the 331 specimens exhibited evidence of VM. Of these 26 VM specimens, 84.6% (22 specimens) exhibited pseudo-comedo formation. RT-PCR analysis of 8 microdissected typical VM specimens revealed expression of Tie-2, Flt-1, thrombin receptor and CD31 in 63, 50, 0 and 0% of specimens, respectively. In contrast, results of RT-PCR analysis of 8 specimens from non-VM tumors were negative for expression of these genes. The 26 VM cases tended to have a higher percentage of hematogenous recurrence (p = 0.059) and a lower percentage of 5-year survival (p = 0.071) than the 305 non-VM cases. However, there were no significant differences in tumor size, lymph node metastasis, estrogen receptors or progesterone receptors between the 2 groups (p > 0.1). Our results suggest that the existence of VM increases the likelihood of hematogenous metastases and is in inverse proportion to prognosis.
Heparin Inhibits DNA Synthesis and Gene Expression in Alveolar Type II Cells (2002)

Responses of isolated type II alveolar cells to fibroblast growth factors (FGF) have been shown to be sensitive to the level of sulfation in extracellular matrix (ECM) substrata. These observations may reflect the specific in situ distribution and level of sulfation of ECM within the alveolar basement membranes (ABM) associated with type II cells. The goal of this study was to determine if the model sulfated ECM heparin modified DNA synthesis and gene expression by type II cells in a concentration dependent-manner. Isolated rat type II cells were exposed to different concentrations of heparin (0.005-500 micro g/ml) in serum-free medium for 1-3 d with or without FGF-1 or FGF-2. The effects of heparin were examined by [(3)H]thymidine incorporation into DNA, total cell protein, cell number, and selected gene expression. Results indicated that heparin inhibited [(3)H]thymidine uptake in a concentration-dependent manner. Total protein, cell number, and FGF-2 protein expression and mRNA of FGF-1, -2, and FGF receptor-2 detected by reverse transcriptase-polymerase chain reaction were decreased by heparin. These results demonstrate that sulfated molecules in the ABM may play important regulatory role(s) in selected type II cell activities during normal cell homeostasis, turnover, and repair after lung injury.
Patterning of rubrene thin-film transistors based on electron irradiation of a polystyrene dielectric layer (2015)

An unprecedented approach to pattern rubrene TFTs is presented by combining an abrupt heating method with selective electron irradiation of polystyrene dielectric layers. The patterned rubrene TFTs exhibited good performances with charge mobilities of ∼1.3 cm<sup>2</sup>V<sup>−1</sup>s<sup>−1</sup>and on/off ratios higher than 10<sup>8</sup>.
Bio‐Based Polyketones by Selective Ring‐Opening Radical Polymerization of α‐Pinene‐Derived Pinocarvone (2015)

The most abundant naturally occurring terpene, α-pinene, which cannot be directly polymerized into high polymers by any polymerization method, was quantitatively converted under visible-light irradiation into pinocarvone, which possesses a reactive exo methylene group. The bicyclic vinyl ketone was quantitatively polymerized in fluoroalcohols by selective (99%) ring-opening radical polymerization of the four-membered ring, which results in unique polymers containing chiral six-membered rings with conjugated ketone units in the main chain. These polymers display good thermal properties, optical activities, and contain reactive conjugated ketone units. Reversible addition fragmentation chain transfer (RAFT) polymerization was successfully accomplished by using appropriate trithiocarbonate RAFT agents, enabling the synthesis of thermoplastic elastomers based on controlled macromolecular architectures.
Cost models for join queries in spatial databases (2002)

The join query is one of the fundamental operations in database management systems (DBMSs). Modern DBMSs should be able to support non traditional data, including spatial objects, in an efficient manner. Towards this goal, spatial data structures can be adopted in order to support the execution of join queries on sets of multidimensional data. The paper introduces analytical models that estimate the cost (in terms of node or disk accesses) of join queries involving two multidimensional indexed data sets using R tree based structures. In addition, experimental results are presented, which show the accuracy of the analytical estimations when compared to actual runs on both synthetic and real data sets. It turns out that the relative error rarely exceeds 15% for all combinations, a fact that makes the proposed cost models useful tools for efficient spatial query optimization.
Lentivirus-meditated frataxin gene delivery reverses genome instability in Friedreich ataxia patient and mouse model fibroblasts (2016)

Friedreich ataxia (FRDA) is a progressive neurodegenerative disease caused by deficiency of frataxin protein, with the primary sites of pathology being the large sensory neurons of the dorsal root ganglia and the cerebellum. FRDA is also often accompanied by severe cardiomyopathy and diabetes mellitus. Frataxin is important in mitochondrial iron-sulfur cluster (ISC) biogenesis and low-frataxin expression is due to a GAA repeat expansion in intron 1 of the FXN gene. FRDA cells are genomically unstable, with increased levels of reactive oxygen species and sensitivity to oxidative stress. Here we report the identification of elevated levels of DNA double strand breaks (DSBs) in FRDA patient and YG8sR FRDA mouse model fibroblasts compared to normal fibroblasts. Using lentivirus FXN gene delivery to FRDA patient and YG8sR cells, we obtained long-term overexpression of FXN mRNA and frataxin protein levels with reduced DSB levels towards normal. Furthermore, γ-irradiation of FRDA patient and YG8sR cells revealed impaired DSB repair that was recovered on FXN gene transfer. This suggests that frataxin may be involved in DSB repair, either directly by an unknown mechanism, or indirectly via ISC biogenesis for DNA repair enzymes, which may be essential for the prevention of neurodegeneration.
Effect of neuromuscular blockade on facial nerve monitoring. (1994)

Direct facial nerve stimulation and monitoring during cerebellopontine angle (CPA) tumor surgery are critical for identification and preservation of function. Electrically evoked facial nerve monitoring was compared with ulnar train-of-four monitoring under progressive neuromuscular blockade. Using a rabbit model, the facial nerve function of six controls was compared to that of six specimens with acute or chronic injuries. Eight of 18 patients who had undergone CPA tumor resection during one year were also studied. Regression analysis correlated between ulnar nerve monitoring and facial electromyographic (EMG) peak voltage in all groups. Facial EMG was measurable, even with 75 percent receptor blockade. The results of this study support the hypothesis that high degrees of neuromuscular blockade do not preclude satisfactory EMG monitoring of the facial nerve during CPA tumor surgery. This study did demonstrate that chronically injured facial nerves may show greater sensitivity to the effects of neuromuscular blockade. Lower levels or avoidance of neuromuscular blockade should be employed under these circumstances.
Defect cluster analysis for wafer-scale integration (1990)

A methodology for characterizing spatial defect distributions is presented. A correlation function approach providing spatial information not measurable with classical methods such as yield-versus-area curves is described. This additional information includes the spatial extent of defect clustering, the strength of clustering, and uncertainty in clustering magnitude. The correlation function methods are applicable to experimentally determined defect maps or to simulation results based on different assumptions concerning the spatial distribution of defects. It is also shown that the approach is useful in predicting yield for redundant circuit configurations when experimental data pertaining to the spatial distribution of defects are available. This type of yield prediction capability is important for judging the feasibility of various redundancy implementations, including wafer scale integration.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Influence of Different Types and Levels of Dietary Lipids on Liver Microsomal Mixed Function Oxidase System in Rats (1990)

It has been shown that dietary polyunsaturated fatty acids stimulate the liver microsomal mixed function oxidase system. The influence of different levels of dietary lard, soybean oil and sardine oil on the mixed function oxidase system was investigated in rats. The diet containing 5% sardine oil rich in eicosapentaenoic and docosahexaenoic acids stimulated the mixed function oxidase system, but the diet containing 5% lard in which lard consisted of 10.7% linolenic acid and 1.5% linolenic acid seemed unlikely to stimulate enough the mixed function oxidase system. On the other hand, no definite effects of large doses of dietary lipids, 25% in the diets, on the mixed function oxidase system were observed.
NEW INSIGHTS INTO β‐ADRENOCEPTORS IN SMOOTH MUSCLE: DISTRIBUTION OF RECEPTOR SUBTYPES and MOLECULAR MECHANISMS TRIGGERING MUSCLE RELAXATION (2005)

1. The beta-adrenoceptor is currently classified into beta(1), beta(2) and beta(3) subtypes and all three subtypes are expressed in smooth muscle. Each beta-adrenoceptor subtype exhibits tissue-specific distribution patterns, which may be a determinant controlling the mechanical functions of corresponding smooth muscle. Airway and uterine smooth muscles abundantly express the beta(2)-adrenoceptor, the physiological significance of which is established as a fundamental regulator of the mechanical activities of these muscles. Recent pharmacomechanical and molecular approaches have revealed roles for the beta(3)-adrenoceptor in the gastrointestinal tract and urinary bladder smooth muscle. 2. The beta-adrenoceptor is a G(s)-protein-coupled receptor and its activation elevates smooth muscle cAMP. A substantial role for a cAMP-dependent mechanism(s) is generally believed to be the key trigger for eliciting beta-adrenoceptor-mediated relaxation of smooth muscle. Downstream effectors activated via a cAMP-dependent mechanism(s) include plasma membrane K(+) channels, such as the large-conductance, Ca(2+)-activated K(+) (MaxiK) channel. 3. Beta-Adrenoceptor-mediated relaxant mechanisms also include cAMP-independent signalling pathways. This view is supported by numerous pharmacological and electrophysiological lines of evidence. In airway smooth muscle, direct activation of the MaxiK channel by G(s)alpha is a mechanism by which stimulation of beta(2)-adrenoceptors elicits muscle relaxation independently of the elevation of cAMP. 4. The cAMP-independent mechanism(s) is also substantial in beta(3)-adrenoceptor-mediated relaxation of gastrointestinal tract smooth muscle. However, in the case of the beta(3)-adrenoceptor, a delayed rectified K(+) channel rather than the MaxiK channel seems to mediate, in part, cAMP-independent relaxant mechanisms. 5. In the present article, we review the distribution of beta-adrenoceptor subtypes in smooth muscle tissues and discuss the molecular mechanisms by which each subtype elicits muscle relaxation, focusing on the roles of cAMP and plasma membrane K(+) channels.
EEG-Based Brain-Controlled Mobile Robots: A Survey (2013)

EEG-based brain-controlled mobile robots can serve as powerful aids for severely disabled people in their daily life, especially to help them move voluntarily. In this paper, we provide a comprehensive review of the complete systems, key techniques, and evaluation issues of brain-controlled mobile robots along with some insights into related future research and development issues. We first review and classify various complete systems of brain-controlled mobile robots into two categories from the perspective of their operational modes. We then describe key techniques that are used in these brain-controlled mobile robots including the brain-computer interface techniques and shared control techniques. This description is followed by an analysis of the evaluation issues of brain-controlled mobile robots including participants, tasks and environments, and evaluation metrics. We conclude this paper with a discussion of the current challenges and future research directions.