gebhart/patrepeval huggingface dataset:
Type II cyclic guanosine monophosphate-dependent protein kinase inhibits Rac1 activation in gastric cancer cells (2015)

Enhanced motility of cancer cells is a critical step in promoting tumor metastasis, which remains the major cause of gastric cancer-associated mortality. The small GTPase Rac1 is a key signaling component in the regulation of cell migration. Previous studies have demonstrated that Rac1 activity may be regulated by protein kinase G (PKG); however, the underlying mechanism is not yet clear. The current study aimed to investigate the effect of type II cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG II) on Rac1 activity. The human gastric cancer cell line AGS was infected with adenoviral constructs encoding PKG II to increase the expression of this enzyme, and treated with a cGMP analog (8-pCPT-cGMP) to induce its activation. A Transwell assay was employed to measure cell migration, and the activity of Rac1 was assessed using a pull-down assay. Immunoprecipitation was used to isolate the Rac1 protein. Phosphorylation of phosphatidylinositol 4,5 bisphosphate 3 kinase (PI3K) and its downstream effecter protein kinase B (Akt) are associated with lysophosphatidic acid (LPA)-induced motility/migration of cancer cells. Extracellular signal regulated kinase (ERK) is the major signaling molecule of the Mitogen activated protein kinase (MAPK) mediated signaling pathway. ERK and its upstream activator MAPK kinase (MEK) are also involved in LPA-induced motility/migration of cancer cells. Phosphorylation of PI3K/Akt, MEK/ERK and enriched Rac1 were detected by western blotting. The results revealed that blocking the activation of Rac1 by ectopically expressing an inactive Rac1 mutant (T17N) impeded LPA-induced cell migration. Increased PKG II activity inhibited LPA-induced migration and LPA-induced activation of Rac1; however, it had no effect on the phosphorylation of Rac1. PKG II also inhibited the activation of PI3K/Akt and MEK/ERK mediated signaling, which is important for LPA-induced Rac1 activation. These results suggest that PKG II affects LPA-stimulated migration of AGS cells by blocking Rac1 activation, via inhibition of PI3K/Akt and MEK/ERK mediated signaling.
Synergy-based Workload Management (2009)

Workload management aims at the efficient execution of queries on a database. In this context, scheduling plays a crucial role. A vast number of scheduling approaches have been developed, most of them belonging to one of two categories: analysis and monitoring. However, they mainly either focus only on one possible kind of impact of queries on each other’s execution time, or require an offline phase for information gathering. In contrast, the approach we pursue does not require any offline phase and flexibly adapts to any database system or hardware configuration. It bases on the fact that the multiple requests that are executed concurrently in a database for performance purposes may have a positive impact on the execution of each other, e.g. due to caching or complementary resource consumption, or impede each other’s execution, e.g. in the case of resource contention. Both kinds of impacts are reflected by the execution time of the workload. We apply a monitoring approach to derive those impacts – called synergies – between request types fully automated at runtime from measured execution times. Thereby, our approach works completely independent from changing synergies or configurations and easily handles new query types. 1.
Lymphocyte transformation studies in suspected hypersensitivity to trimethoprim‐sulphamethoxazole (1983)

In a group of patients with suspected and probable adverse skin reactions to trimethoprim-sulphamethoxazole, the lymphocyte transformation test (LTT) was positive in approximately 60% of subjects tested. Levels of stimulation in the majority of cases were low, probably reflecting either use of inappropriate stimulatory forms of the drug or lack of marked reactivity. Since there appeared to be a correlation between peak stimulatory index and duration of the rash, the latter explanation appeared more likely.
Absolute Beginner's Guide to Quattro Pro X (Absolute Beginner's Guide) (2005)

More and more people are learning the advantages of using Corel's Quattro Pro over other spreadsheet applications.i� If you have joined their ranks, this book is for you!i� Absolute Beginner's Guide to Quattro Pro X3i� is endorsed by Corel, and offers a straight-forward, no-nonsense approach to the basics.i� With the help of this book, you will learn how to efficiently manage, analyze, report, and share data, and then move on to more advanced features. i�
Post-Marketing Surveillance of Euhypnos® (Temazepam): A New Hypnotic (1980)

A post-marketing surveillance study of Euhypnos (temazepam), a new short-acting benzodiazepine hypnotic. A total of 12,350 patients requiring a sleep inducer were treated for up to 3 months with doses of 10-30 mg at night. After 2 weeks 80% of First Reports (FRs) rated Euhypnos effective and at 3 months this had risen to 92% of 3062 Second Reports (SRs). Hangover was reported in 7% of FRs and 2% of SRs but in general the drug was well tolerated with adverse reactions consisting mainly of morning nausea, headache, drowsiness and vivid dreaming. Eighty-seven per cent of FRs and 93% of SRs were 'Clean' reporting no hangover, adverse reaction or event of any kind.
Radiation-induced chromosome breaks in ataxia-telangiectasia cells remain open (2003)

The disability of cells to rejoin broken chromosome ends does not lead to the healing of DSBs by the acquisition of new telomeric sequences.
A language modelling approach to relevance profiling for document browsing (2002)

This paper describes a novel tool, SmartSkim, for content-based browsing or skimming of documents. The tool integrates concepts from passage retrieval and from interfaces, such as TileBars, which provide a compact overview of query term hits within a document. We base our tool on the concept of relevance profiling, in which a plot of retrieval status values at each word position of a document is generated. A major contribution of this paper is applying language modelling to the task of relevance profiling. We describe in detail the design of the SmartSkim tool, and provide a critique of the design. Possible applications of the tool are described, and we consider how an operational version of SmartSkim might be designed.
Sweeping the Unclean: Social Media and the Bersih Electoral ReformMovement in Malaysia (2016)

In this article author investigate how social media was utilized and appropriated in the electoral reform movement in Malaysia called Bersih. By identifying and analyzing roles of three dominant social platforms in the Bersih movement, namely blogging, Facebook, and Twitter, author reveal that social media is both the site and part of the contestations of power Social media is integral to the shaping of Bersih movement's imaginaries, practices, and trajectories. As a social and material artifact, every technological platform such as blogging, Facebook, and Twitter has its own socio-political properties that postulate distinctive roles and limitations for its users.
The SXYQRL sequence in the cytoplasmic domain of TGN38 plays a major role in trans-Golgi network localization. (1993)

The trans-Golgi network (TGN) plays a central role in protein sorting/targeting. TGN38 is an integral membrane protein confined mainly to the TGN. When the cytoplasmic 32-residue sequence of TGN38 was fused to the ecto- and transmembrane domains of glycophorin A (a surface protein), the resulting chimeric protein was localized to the TGN. Detailed mutagenesis of the 32-residue sequence revealed that the Ser, Tyr, and Leu residues at positions 23, 25, and 28, respectively, are essential for TGN localization. Further studies demonstrate that the sequence SXYQRL can by itself confer significant TGN localization.
Konjac glucomannan-based hydrogel with hyaluronic acid as a candidate for a novel scaffold for chondrocyte culture (2009)

Chondrocytes were cultured using konjac glucomannan (KGM) and hyaluronic acid (HA) as a scaffold for cartilage regeneration. They were subsequently compared with scaffolds produced using agarose hydrogels. Chondrocytes derived from Japanese white rabbits were cultured: 2.0 x 10(5) cells were seeded on KGM containing hyaluronic acid (KGM/HA) and agarose and cultured for 5 days. Their viability was assayed using WST-8 procedures; the ultimate stress and modulus of elasticity of each construct was calculated. After 3 days of cultivation, mRNA in chondrocytes, such as collagen types I and II and aggrecan, were measured using RT-PCR. Both chondrocyte-seeded constructs were stained with safranin O/fast green and were evaluated histologically. Chondrocyte viability decreased concomitantly with increasing KGM/HA or agarose concentration and with culture time. Cell viability in 2% agarose was significantly lower than that in 2% KGM/HA on the third and fifth days (p < 0.05). The primary elastic modulus increased concomitantly with increasing polysaccharide concentration. Elastic moduli of 2% KGM/HA with chondrocytes (0.389 +/- 0.119 N/mm(2)) showed little difference from those without chondrocytes (0.283 +/- 0.243 N/mm(2)), although those of 2% agarose with chondrocytes (0.403 +/- 0.094 N/mm(2)) were significantly lower than those without chondrocytes (0.736 +/- 0.227 N/mm(2); p < 0.05). Collagen type II mRNA expression was higher in KGM/HA and agarose than in monolayer cultures, although KGM/HA had lower aggrecan mRNA expression levels than did agarose. Histological tests of KGM/HA-chondrocyte constructs revealed chondrocyte aggregation and proteoglycan production in the pericellular region. The results show that KGM/HA might be useful for chondrocyte culture.
Rapid assembly of multilayer microfluidic structures via 3D-printed transfer molding and bonding (2016)

A critical feature of state-of-the-art microfluidic technologies is the ability to fabricate multilayer structures without relying on the expensive equipment and facilities required by soft lithography-defined processes. Here, three-dimensional (3D) printed polymer molds are used to construct multilayer poly(dimethylsiloxane) (PDMS) devices by employing unique molding, bonding, alignment, and rapid assembly processes. Specifically, a novel single-layer, two-sided molding method is developed to realize two channel levels, non-planar membranes/valves, vertical interconnects (vias) between channel levels, and integrated inlet/outlet ports for fast linkages to external fluidic systems. As a demonstration, a single-layer membrane microvalve is constructed and tested by applying various gate pressures under parametric variation of source pressure, illustrating a high degree of flow rate control. In addition, multilayer structures are fabricated through an intralayer bonding procedure that uses custom 3D-printed stamps to selectively apply uncured liquid PDMS adhesive only to bonding interfaces without clogging fluidic channels. Using integrated alignment marks to accurately position both stamps and individual layers, this technique is demonstrated by rapidly assembling a six-layer microfluidic device. By combining the versatility of 3D printing while retaining the favorable mechanical and biological properties of PDMS, this work can potentially open up a new class of manufacturing techniques for multilayer microfluidic systems.
What’s New in Gastric Cancer: The Therapeutic Implications of Molecular Classifications and Future Perspectives (2018)

Despite some remarkable innovations and the advent of novel molecular classifications the prognosis of patients with advanced gastric cancer (GC) remains overall poor and current clinical application of new advances is disappointing. During the last years only Trastuzumab and Ramucirumab have been approved and currently used as standard of care targeted therapies, but the systemic management of advanced disease did not radically change in contrast with the high number of molecular drivers identified. The Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG) classifications paved the way, also for GC, to that more contemporary therapeutic approach called "precision medicine" even if tumor heterogeneity and a complex genetic landscape still represent a strong barrier. The identification of specific cancer subgroups is also making possible a better selection of patients that are most likely to respond to immunotherapy. This review aims to critically overview the available molecular classifications summarizing the main druggable molecular drivers and their possible therapeutic implications also taking advantage of new technologies and acquisitions.
Damage behaviour of an Al<sub>2</sub>O<sub>3</sub> particle-reinforced 6061 alloy induced by monotonic and cyclic deformation (2006)

Abstract The influence of damage and its correlation with the failure behaviour is discussed in the light of microstuctural aspects. The failure induced by microstructural mechanisms of the Al 2 O 3 -particle reinforced aluminium alloy 6061 under uniaxial tensile as well as under cyclic loading was investigated. The damage behaviour as a function of the strain ε can be correlated with stiffness E (ε). Under cyclic loading conditions the damage increases with the number of cycles and can be correlated with the strain amplitude ε a . Furthermore, the damage is associated with crack formation and propagation and the damage evolution depends sensitively on the kind of deformation. The crack formation was detected by metallographic investigations and calculated by numerical methods (FEM), taking the size and shape of particles into account. Generally, damage under mechanical loading is initiated by broken particles and agglomerations of particles. In case of the particulate-reinforced specimens the evolution of damage is quite different for monotonic and cyclic loading conditions but, nevertheless, in both cases the damage parameter is given by inhomogeneities characteristic for the MMC which act as seeds for crack initiation.
Application of the method of rank annihilation to quantitative analyses of multicomponent fluorescence data from the video fluorometer (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTApplication of the method of rank annihilation to quantitative analyses of multicomponent fluorescence data from the video fluorometerC. N. Ho, G. D. Christian, and E. R. DavidsonCite this: Anal. Chem. 1978, 50, 8, 1108–1113Publication Date (Print):July 1, 1978Publication History Published online1 May 2002Published inissue 1 July 1978https://doi.org/10.1021/ac50030a026RIGHTS & PERMISSIONSArticle Views259Altmetric-Citations219LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (626 KB) Get e-Alerts Get e-Alerts
Toward a strategic framework of mobile knowledge management (2008)

Abstract Organizations are challenged to seek efficient and effective ways of implementing knowledge management strategies in an increasingly mobile environment. Building upon our CLIC (conservation, leverage, integration, and combination) framework of mobile knowledge management (mKM) strategies, we investigate the implementation of mKM strategies from a system perspective. An attempt is also made to formulate a decision table based upon three dimensions of knowledge properties (tacitness, location, and mobility) and then the best knowledge management strategy under each scenario is proposed. Finally, different types of hierarchies of mKM systems are identified and the selection of an appropriate hierarchy for different types of companies is elaborated. The paper provides a solid foundation for future research in mKM as well as a valuable guidance for practical managers to strategically manage mobile knowledge assets. Copyright © 2008 John Wiley & Sons, Ltd.
Tailoring ZSM‐5 Zeolites for the Fast Pyrolysis of Biomass to Aromatic Hydrocarbons (2016)

The production of aromatic hydrocarbons from cellulose by zeolite-catalyzed fast pyrolysis involves a complex reaction network sensitive to the zeolite structure, crystallinity, elemental composition, porosity, and acidity. The interplay of these parameters under the reaction conditions represents a major roadblock that has hampered significant improvement in catalyst design for over a decade. Here, we studied commercial and laboratory-synthesized ZSM-5 zeolites and combined data from 10 complementary characterization techniques in an attempt to identify parameters common to high-performance catalysts. Crystallinity and framework aluminum site accessibility were found to be critical to achieve high aromatic yields. These findings enabled us to synthesize a ZSM-5 catalyst with enhanced activity, which offers the highest aromatic hydrocarbon yield reported to date.
A text retrieval approach to content-based audio retrieval (2008)

This paper presents a novel approach to robust, contentbased retrieval of digital music. We formulate the hashing and retrieval problems analogously to that of text retrieval and leverage established results for this unique application. Accordingly, songs are represented as a ”Bagof-Audio-Words” and similarity calculations follow directly from the well-known Vector Space model [12]. We evaluate our system on a 4000 song data set to demonstrate its practical applicability, and evaluation shows our technique to be robust to a variety of signal distortions. Most interestingly, the system is capable of matching studio recordings to live recordings of the same song with high accuracy. 1
<i>trans</i> -Complementation Analysis of the Flavivirus Kunjin ns5 Gene Reveals an Essential Role for Translation of Its N-Terminal Half in RNA Replication (1999)

Recently we described rescue of defective Kunjin virus (KUN) RNAs with small deletions in the methyltransferase and RNA polymerase motifs of the ns5 gene, using BHK cells stably expressing KUN replicon RNA (repBHK cells) as helper (A. A. Khromykh et al., J. Virol. 72:7270-7279, 1998). We have now extended our previous observations and report successful trans-complementation of defective KUN RNAs with most of the ns5 gene deleted or substituted with a heterologous (dengue virus) ns5 sequence. Replication of full-length KUN RNAs with 3'-terminal deletions of 136 (5%), 933 (34%), and 1526 (56%) nucleotides in the ns5 gene was complemented efficiently in transfected repBHK cells. RNA with a larger deletion of 2,042 nucleotides (75%) was complemented less efficiently, and RNA with an even larger deletion of 2,279 nucleotides (84%) was not complemented at all. Chimeric KUN genomic RNA containing 87% of the KUN ns5 gene replaced by the corresponding sequence of the dengue virus type 2 ns5 gene was unable to replicate in normal BHK cells but was complemented in repBHK cells. These results demonstrate for the first time complementation of flavivirus RNAs with large deletions (as much as 75%) in the RNA polymerase gene and establish that translation of most of the N-terminal half of NS5 is essential for complementation in trans. A model of formation of the flavivirus replication complex implicating a possible role in RNA replication of conserved coding sequences in the N-terminal half of NS5 is proposed based on the complementation and earlier results with KUN and on reported data with other flaviviruses.
Analysis and measurements on a PWM DC-DC converter with lossless snubbers (2002)

Analysis and measurements are used to demonstrate the advantages and disadvantages of a promising DC-DC power converter topology with lossless snubbers. With the snubbers the total transistor and diode losses are shown to be much less than in hard switching when the load current is large, and equal to or less than in hard switching when the load current is small. Measurements of the insulated-gate bipolar transistor (IGBT) switching performance in the DC-DC converter are presented. The design of a converter transformer with an integrated inductor is also presented. Also examined is the optimum gate resistance with respect to the performance when the snubber capacitors are not fully discharged at turn-on of the transistors.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Validation Results of Specifications for Motion Control Interoperability (1996)

The National Institute of Standards and Technology (NIST) is participating in the Department of Energy Technologies Enabling Agile Manufacturing (TEAM) program to establish interface standards for machine tool, robot, and coordinate measuring machine controllers. At NIST, the focus is to validate potential application programming interfaces (APIs) that make it possible to exchange machine controller components with a minimal impact on the rest of the system. This validation is taking place in the enhanced machine controller (EMC) consortium and is in cooperation with users and vendors of motion control equipment. An area of interest is motion control, including closed-loop control of individual axes and coordinated path planning. Initial tests of the motion control APIs are complete. The APIs were implemented on two commercial motion control boards that run on two different machine tools. The results for a baseline set of APIs look promising, but several issues were raised. These include resolving differing approaches in how motions are programmed and defining a standard measurement of performance for motion control. This paper starts with a summary of the process used in developing a set of specifications for motion control interoperability. Next, the EMC architecture and its classification of motion control APIs into two classes, Servo Control and Trajectory Planning, are reviewed. Selected APIs are presented to explain the basic functionality and some of the major issues involved in porting the APIs to other motion controllers. The paper concludes with a summary of the main issues and ways to continue the standards process.
Opportunities and challenges in developing deep learning models using electronic health records data: a systematic review (2018)

Despite the early success in using deep learning for health analytics applications, there still exist a number of issues to be addressed. We discuss them in detail including data and label availability, the interpretability and transparency of the model, and ease of deployment.