gebhart/patrepeval huggingface dataset:
Étude des procédés d’extraction et de purification de produits bioactifs à partir de plantes par couplage de techniques séparatives à basses et hautes pressions (2010)

This thesis deals with the extraction of natural bioactive compounds from plants (case study with Lemon Balm (Melissa officinalis L.)) by using different separation techniques at high (supercritical extraction) and atmospheric pressure (Soxhlet extraction, batch extraction, nanofiltration etc.). The influence of main operational parameters (solvent composition and flow rate, particle size of the raw material, pressure, temperature) on the process kinetics is studied experimentally with the aim to determine appropriate operational conditions for better extraction. The experimental results are confronted to a number of mathematical models in order to estimate the applicability of different theoretical concepts to the particular process and to select and apply appropriate models for determination of important parameters, characterizing the mass transfer process and necessary for scale-up and design purposes. Coupling between different separation methods is also considered and a number of integrated process schemes are proposed resulting in better yield of the targeted compounds.
Developmental studies of an adaptive on‐line softsensor for biological wastewater treatments (1999)

Abstract Efficient monitoring and automatic control systems for biological wastewater treatment processes, especially those dealing with bioinhibitory pollutants, such as phenol, are urgently required in order to meet increasingly stringent environmental regulations. Practical on‐line sensors of variables that describe water quality, such as BOD or individual toxic pollutants such as phenol, are not commercially available; e.g. phenol is generally monitored off‐line by spectrofluorometry. Inference software sensors could be an attractive alternative for on‐line monitoring of these variables. As a first step towards the development of inferential sensors for biological wastewater treatment processes, we consider in this study, a simplistic version of such a process which consists of a continuous culture of Pseudomonas putida Q5 degrading phenol. In this work, we propose a neural network based inferential sensor for phenol monitoring using on‐line biomass concentration measurements by spectrophotometry. The network was built with wavelets as the basis functions and the adaptive algorithm for the weights was based on a Lyapunov stability analysis. Predicted phenol output of the network showed good agreement with experimental data, over fairly broad ranges of inlet phenol concentration and dilution rate step changes. Simulations were conducted to find convergence conditions and to investigate possible sources for errors in phenol estimates.
3D Images Using CSpace Display for Air Traffic Control Applications (2011)

We explore here the capabilities and performance issues encountered when using a novel static volume display, CSpace, for realizing moving images required to deliver real time three-dimensional (3D) visualization and manipulation for Air Traffic Controllers (ATC). The display uses a 19 mm ×18 mm × 100 mm 2% Erbium-doped sodium-ytterbium-fluoride (NYF) crystal as the image space, and a pair of digital micromirror devices (DMD) to address and render successive slices of the image within the crystal. Display-specific software and digital light processing (DLP) boards provide image data to and control the DMD devices respectively. The ability to render and display moving images quickly enough for applications such as air-traffic control is strongly dependent on several factors, including the speed of the software and hardware components. 3D images of aircraft in the sky and terrain were captured to explore how well the display performed as the time between successive images in a short motion sequence was systematically reduced, and to examine the key factors that control the performance of the display in presenting these images.
Cross-regulation between G-protein-coupled receptors. Activation of beta 2-adrenergic receptors increases alpha 1-adrenergic receptor mRNA levels (1991)

Stimulation of DDT1 MF-2 vas deferens cells with epinephrine resulted in a time- and dose-dependent loss of alpha 1-adrenergic receptor-specific ligand binding. Regulation of alpha 1-adrenergic receptor mRNA was characterized. In monolayer culture, cells displayed 0.7 +/- 0.05 amol of alpha 1-adrenergic receptor mRNA/microgram of total cellular RNA. Epinephrine, which acts at both alpha 1- and beta 2-adrenergic receptors of DDT1 MF-2 cells, induced a short term (2-8 h) increase (50-70%) in the abundance of alpha 1-adrenergic receptor mRNA. Propranolol, a beta 2-adrenergic receptor antagonist, attenuated the epinephrine-mediated increase in alpha 1-adrenergic receptor mRNA but did not affect the decrease in alpha 1-adrenergic receptor-specific ligand binding. Phentolamine, an alpha 1-adrenergic receptor antagonist, did not attenuate the epinephrine-mediated increase in alpha 1-adrenergic receptor mRNA at 4 h but did block the decrease in alpha 1-adrenergic receptor-specific ligand binding. The half-life of the alpha 1-adrenergic receptor mRNA was approximately 7 h in untreated cells as well as in cells challenged with epinephrine. The epinephrine-promoted increase in alpha 1-adrenergic receptor mRNA was found to result from cross-regulation via beta 2-adrenergic receptors. Cholera toxin, forskolin, as well as the cyclic AMP analog CPT cAMP (8-(4-chlorophenylthio)adenosine 3':5'-cyclic monophosphate) increased the alpha 1-adrenergic receptor mRNA at 4 h, as did epinephrine in the presence of alpha 1-antagonists but not in the presence of a beta-adrenergic antagonist. This is the first report of heterologous up-regulation of mRNA levels of adrenergic receptors. Cross-regulation between alpha 1- and beta 2-adrenergic receptor-mediated pathways at 4 h occurs at the level of mRNA whereas later down-regulation of alpha 1-receptor mRNA and binding proceed via agonist activation of alpha 1-adrenergic receptors.
Self-Assembly of Heme A and Heme B in a Designed Four-Helix Bundle:  Implications for a Cytochrome <i>c</i> Oxidase Maquette (2000)

Heme A, a prosthetic group of cytochrome c oxidase [EC 1.9.3.1], has been introduced into two de novo designed four helix bundle proteins, [H10A24](2) and [H10H24](2), known to bind 2-4 equiv of heme B, respectively [Robertson, D. E., Farid, R. S., Moser, C. C., Mulholland, S. E., Pidikiti, R., Lear, J. D., Wand, A., J., DeGrado, W. F., and Dutton, P. L. (1994) Nature 368, 425-432]. [H10A24](2), [Ac-CGGGELWKL x HEELLKK x FEELLKL x AEERLKK x L-CONH(2)](2)(2), binds two heme A molecules per four-helix unit via bis-histidine ligation at the 10,10' positions with measured K(d) values of <0.1 and 5 nM, values much lower than those measured for heme B (K(d) values of 50 and 800 nM). The heme A-protein complex, [heme A-H10A24](2), exhibits well-defined absorption spectra in both the ferric and ferrous states, and an electron paramagnetic resonance spectrum characteristic of a low spin heme in the ferric form. A single midpoint redox potential (E(m8)) was determined for [heme A-H10A24](2) at -45 mV (vs SHE), which is significantly higher than that of the protein bound heme B (-130 and -200 mV). The observation of a single midpoint redox potential for [heme A-H10A24](2) and a pair of midpoints for [heme B-H10A24](2) indicates that the di-alpha-helical monomers are oriented in an anti topology (disulfides on opposite sides of bundle) in the former (lacking heme-heme electrostatic interaction) and syn in the latter. A mixture of global topologies was indicated by the potentiometric titration of the related [heme A-H10H24](2) which possess two distinct reduction potentials of +41 (31%) and -65 mV (69%). Self-assembly of the mixed cofactor heme A-heme B-[H10A24](2) was accomplished by addition of a single equivalent of each heme A and heme B to [H10A24](2). The single midpoint redox potential of heme B, E(m8) = -200 mV, together with the split midpoint redox potential of heme A in heme A-heme B-[H10A24](2), E(m8) = +28 mV (33%) and -65 mV (67%), indicated the existence of both syn and anti topologies of the two di-alpha-helical monomers in this four helix bundle. Synthesis of the mixed cofactor [heme A-heme B-H10H24](2) was accomplished by addition of a 2 equiv of each heme A and heme B to [H10H24](2) and potentiometry indicated the pair of hemes B resided in the 10,10' sites and heme A occupied the 24,24' sites. The results indicate that heme peripheral structure controls the orientation of the di-alpha-helical monomers in the four-helix bundle which are interchangeable between syn and anti topologies. In the reduced form, [heme A-H10A24](2), reacts quantitatively to form [carbonmonoxy-heme A-H10A24](2) as evidenced by optical spectroscopy. The synthetic [heme A-H10A24](2) can be enzymatically reduced by NAD(P)H with natural reductases under anaerobic conditions, and reversibly oxidized by dioxygen to the ferric form.
Anomalous Orientation-Dependent Effective Pair Interaction among Histidine and Other Amino Acid Residues in Metalloproteins:  Breakdown of the Hydropathy Scale Index (2006)

Hydropathy scale is widely used to obtain a measure of the effective interaction between any two amino acid residues in proteins and is based on the assumption that attraction between two hydrophobic groups and repulsion between hydrophilic groups (in water) can be translated straightforwardly to protein environment. Here we employ a recently developed statistical mechanical approach combined with the Protein Data Bank to obtain both distance- and orientation-dependent potential of mean force (ODPMF). This allowed us to explore effective pair potential among many amino acid residues and to examine the validity of the hydropathy scale in modeling the interaction among amino acid residues. We find that in some cases, like Phe-Phe and Lys-Lys, the hydropathy scale approach is largely obeyed. However, we also observe many unexpected pair interactions which defy the trend given by published hydropathy scales. An example of the former is the arginine-arginine (Arg-Arg) pair interaction which is found to be strongly and surprisingly attractive at short separation, even though it is the most hydrophilic residue. Here the head-to-head (see text) interaction is also stabilized. Tryptophan residues also exhibit strong attractive interaction. Equally important, we find strong influence of metal in determining effective interaction among the amino acid residues. It is the behavior of the histidine (His) which is found to be the most unusual. It exhibits a strong attractive interaction with itself which gets significantly enhanced in metalloproteins. These results highlight the important (sometime hidden) role of metals in protein structure and folding.
THE CLINICAL IMPORTANCE OF CHOOSING THE RIGHT ASSAY FOR DETECTION OF HLA-SPECIFIC DONOR-REACTIVE ANTIBODIES1 (1999)

Assays to determine the specificity of donor-reactive antibodies are now available for use during an acute transplant situation. The introduction of such methods is expected to enhance graft survival and to reduce significantly the frequencies of acute rejections episodes after a transplant.
On the dynamics of buoyant and heavy particles in a periodic Stuart vortex flow (1993)

In this paper, we study the dynamics of small, spherical, rigid particles in a spatially periodic array of Stuart vortices given by a steady-state solution to the two-dimensional incompressible Euler equation. In the limiting case of dominant viscous drag forces, the motion of the particles is studied analytically by using a perturbation scheme. This approach consists of the analysis of the leading-order term in the expansion of the ‘particle path function’ Φ, which is equal to the stream function evaluated at the instantaneous particle position. It is shown that heavy particles which remain suspended against gravity all move in a periodic asymptotic trajectory located above the vortices, while buoyant particles may be trapped by the stable equilibrium points located within the vortices. In addition, a linear map for Φ is derived to describe the short-term evolution of particles moving near the boundary of a vortex. Next, the assumption of dominant viscous drag forces is relaxed, and linear stability analyses are carried out to investigate the equilibrium points of the five-parameter dynamical system governing the motion of the particles. The five parameters are the free-stream Reynolds number, the Stokes number, the fluid-to-particle mass density ratio, the distribution of vorticity in the flow, and a gravitational parameter. For heavy particles, the equilibrium points, when they exist, are found to be unstable. In the case of buoyant particles, a pair of stable and unstable equilibrium points exist simultaneously, and undergo a saddle-node bifurcation when a certain parameter of the dynamical system is varied. Finally, a computational study is also carried out by integrating the dynamical system numerically. It is found that the analytical and computational results are in agreement, provided the viscous drag forces are large. The computational study covers a more general regime in which the viscous drag forces are not necessarily dominant, and the effects of the various parametric inputs on the dynamics of buoyant particles are investigated.
A Ratiometric Fluorescent Probe for Hypochlorite Based on a Deoximation Reaction (2009)

In this work, we have successfully provided a novel strategy for the rational design and synthesis of a ratiometric fluorescent probe for hypochlorite. The strategy is based on the deoximation reaction, which has not yet been used in the fluorescent hypochlorite probe design. Interestingly, the probe showed a ratiometric fluorescent response to hypochlorite with the emission intensities ratio (I(509)/I(439)) increasing from 0.28 to 2.74. Furthermore, the probe displayed high selectivity for hypochlorite over other species due to the distinct deoximation conditions. The probe developed herein represents the first ratiometric fluorescent probe for hypochlorite.
Long-Term Antidepressant Efficacy and Safety of Olanzapine/Fluoxetine Combination (2003)

The olanzapine/fluoxetine combination showed rapid, robust, and sustained improvement in depressive symptoms in patients with MDD, including patients with TRD. The long-term safety profile of the combination was similar to that of its component monotherapies.
PCR Amplification of Wheat Sequences from DNA Extracted During Milling and Baking (2004)

ABSTRACT DNA‐based analyses are highly sensitive and specific. Because processing steps can have profound effects on the proteins and DNA present in foods, this project examined the effects of breadmaking on wheat DNA size and polymerase chain reaction (PCR)‐based detection of sequences. DNA was extracted from wheat kernels, milling fractions, and flour, and from samples taken at various steps during and after the baking process. Kernels contained primarily high molecular weight DNA (>12,000 base pairs [bp]), whereas flour DNA exhibited a broad range of molecular weights from >12,000 bp to <300 bp. A marked reduction in DNA yield and size occurred after the first 5 min of baking. PCR successfully amplified products of both high and low copy number genes, even from DNA extracted from bread loaves five days after baking. However, successful amplification required that the maximum product size be no more than the average molecular weight of the DNA recovered from the source. The data also demonstrate that PCR can be used to detect the presence of yeast ( Saccharomyces cerevisiae ), a minor ingredient.
Reuse historic costs in dynamic programming to reduce computational complexity in the context of model predictive optimization (2015)

Energy efficiency has become a major issue in trade, transportation and environment protection. While the next generation of zero emission propulsion systems still have difficulties in reaching similar travel distances as combustion engine propulsion systems, it is already possible to increase fuel efficiency in regular vehicles by applying a more fuel efficient driving behaviour. An adapted Dynamic Programming approach is used to calculate optimal behaviour profiles for the road ahead within a finite optimization horizon. The main purpose of this publication is the development of a strategy to reuse historic minimal costs in order to reduce the computational complexity of future optimization steps. The percent reduction is deterministic and increases with the discretization degree of the optimization horizon.
Activation of the IGF1R pathway potentially mediates acquired resistance to mutant-selective 3rd-generation EGF receptor tyrosine kinase inhibitors in advanced non-small cell lung cancer (2016)

Mutant-selective, 3rd-generation EGFR-TKIs were recently developed to control lung cancer cells harboring T790M-mediated resistance. However, the development of resistance to these novel drugs seems inevitable. Thus, we investigated the mechanism of acquired resistance to the mutant-selective EGFR-TKI WZ4002. We established five WZ4002-resistant cells, derived from cells harboring both EGFR and T790M mutations by long-term exposure to increasing doses of WZ4002. Compared with the parental cells, all resistant cells showed 10-100-folds higher resistance to WZ4002, as well as cross-resistance to other mutant-selective inhibitors. Among them, three resistant cells (HCC827/WR, PC-9/WR and H1975/WR) showed dependency on EGFR signaling, but two other cells (PC-9/GR/WR and PC-9/ER/WR) were not. Notably, insulin-like growth factor-1 receptor (IGF1R) was aberrantly activated in PC-9/GR/WR cells in phospho-receptor tyrosine kinase array, consistently accompanied by loss of IGF binding protein-3 (IGFBP3). Down-regulation of IGF1R by shRNA, as well as inhibition of IGF1R activity either by AG-1024 (a small molecule IGF1R inhibitor) or BI 836845 (a monoclonal anti-IGF1/2 blocking antibody), restored the sensitivity to WZ4002 both in vitro and xenograft. Taken together, these results suggest that activation of the IGF1R pathway associated with IGFBP3 loss can induce an acquired resistance to the mutant-selective EGFR-TKI, WZ4002. Therefore, a combined therapy of IGF1R inhibitors and mutant-selective EGFR-TKIs might be a viable treatment strategy for overcoming acquired resistance.
Involvement of Aquaporin 9 in Osteoclast Differentiation (2006)

Aquaporins (water channels) selectively enhance water permeability of membranes. Since osteoclast differentiation includes a dramatic increase in cell volume, we hypothesize that aquaporin(s) is/are critical for the formation of the multinucleated osteoclast from its mononuclear precursor. Our studies employ two cell models, bone marrow macrophages (BMMs) and the murine macrophage-like cell line, RAW264.7, as osteoclast precursors. Receptor activator of nuclear factor kappaB (NF-kappaB) ligand (RANKL) and macrophage-colony-stimulating factor or RANKL alone were used to induce osteoclast differentiation in BMMs or RAW264.7 cells, respectively. We first used qualitative reverse transcription (RT)-PCR to examine which of the aquaporins are expressed in osteoclasts and in their precursor cells. Out of the 10 aquaporins examined, only aquaporin 9 (AQP9) was expressed in osteoclast-lineage cells. AQP9 has unique aqueous pore properties mediating the passage of a wide variety of non-charged solutes in addition to water. Western analyses using specific antibodies revealed a higher AQP9 level in RANKL-treated than in untreated cells. Quantitative real-time RT-PCR analyses also demonstrated higher AQP9 mRNA levels in RANKL-treated cells. Finally, we examined the effect of phloretin, an AQP9 inhibitor, on RANKL-induced osteoclast differentiation. Cells were incubated with RANKL for 5 days, and phloretin was added for the last 2 days, when most fusion occurs. A dramatic reduction in osteoclast size and in the number of nuclei per osteoclast was observed in cultures containing phloretin. The inhibitor did not have a significant effect on the number and size of mononuclear phagocytes in cultures not treated with RANKL. Our results suggest a role for AQP9 in osteoclast differentiation, specifically in the fusion process.
A Practical Approach to Classify Evolving Data Streams: Training with Limited Amount of Labeled Data (2008)

Recent approaches in classifying evolving data streams are based on supervised learning algorithms, which can be trained with labeled data only. Manual labeling of data is both costly and time consuming. Therefore, in a real streaming environment, where huge volumes of data appear at a high speed, labeled data may be very scarce. Thus, only a limited amount of training data may be available for building the classification models, leading to poorly trained classifiers. We apply a novel technique to overcome this problem by building a classification model from a training set having both unlabeled and a small amount of labeled instances. This model is built as micro-clusters using semi-supervised clustering technique and classification is performed with kappa-nearest neighbor algorithm. An ensemble of these models is used to classify the unlabeled data. Empirical evaluation on both synthetic data and real botnet traffic reveals that our approach, using only a small amount of labeled data for training, outperforms state-of-the-art stream classification algorithms that use twenty times more labeled data than our approach.
A reply to comments by Moore [J. Acoust. Soc. Am. <b>7</b> <b>8</b>, 253–255 (1985)] (1985)

Lutfi’s [J. Acoust. Soc. Am. 76, 1045–1050 (1984)] model predicts the results of studies cited by Moore [J. Acoust. Soc. Am. 78, xxx-xxx (1985)]. Differences between simultaneous and forward masked measures of frequency selectivity can be accounted for without involving additional tuning mechanisms.
Proportional Navigation with a Maneuvering Target (1972)

A qualitative analysis of the trajectories of a missile pursuing a maneuvering target according to the proportional navigation law is presented. Conditions for a missile to reach the target from any initial state are determined. The existence of a boundary for the required missile acceleration is demonstrated.
A speaker tracking system based on speaker turn detection for NIST evaluation (2002)

A speaker tracking system (STS) is built by using successively a speaker change detector and a speaker verification system. The aim of the STS is to find in a conversation between several persons (some of them having already enrolled and other being totally unknown) target speakers chosen in a set of enrolled users. In a first step, speech is segmented into homogeneous segments containing only one speaker, without any use of a priori knowledge about speakers. Then, the resulting segments are checked to belong to one of the target speakers. The system has been used in a NIST evaluation test with satisfactory results.
Tapped-Line Coupled Transmission Lines with Applications to Interdigital and Combline Filters (1975)

Exact, general, open-wire-line equivalent circuits for tapped-line combline and interdigital arrays are derived using a combination of graph transformations and induction. A significant feature of the equivalent circuits is that they do not require commensurate length sections. The equivalent circuit for tapped-line interdigital arrays is utilzed to develop design equations for tapped-line interdigital filters.