gebhart/patrepeval huggingface dataset:
NEDD8-Targeting Drug MLN4924 Elicits DNA Rereplication by Stabilizing Cdt1 in S Phase, Triggering Checkpoint Activation, Apoptosis, and Senescence in Cancer Cells (2010)

MLN4924 is a first-in-class experimental cancer drug that inhibits the NEDD8-activating enzyme, thereby inhibiting cullin-RING E3 ubiquitin ligases and stabilizing many cullin substrates. The mechanism by which MLN4924 inhibits cancer cell proliferation has not been defined, although it is accompanied by DNA rereplication and attendant DNA damage. Here we show that stabilization of the DNA replication factor Cdt1, a substrate of cullins 1 and 4, is critical for MLN4924 to trigger DNA rereplication and inhibit cell proliferation. Even only 1 hour of exposure to MLN4924, which was sufficient to elevate Cdt1 for 4-5 hours, was found to be sufficient to induce DNA rereplication and to activate apoptosis and senescence pathways. Cells in S phase were most susceptible, suggesting that MLN4924 will be most toxic on highly proliferating cancers. Although MLN4924-induced cell senescence seems to be dependent on induction of p53 and its downstream effector p21(Waf1), we found that p53(-/-) and p21(-/-) cells were even more susceptible than wild-type cells to MLN4924. Our results suggested that apoptosis, not senescence, might be more important for the antiproliferative effect of MLN4924. Furthermore, our findings show that transient exposure to this new investigational drug should be useful for controlling p53-negative cancer cells, which often pose significant clinical challenge.
Closed-form expressions for interconnection delay, coupling, and crosstalk in VLSIs (1993)

A closed-form formula for a waveform of the RC interconnection line with practical boundary conditions is derived. Expressions are also derived for the voltage slope and transition time of the RC interconnection and for coupling capacitance and crosstalk voltage height, which can be used in VLSI designs. Using the expressions, the optimum linewidth that minimizes RC delay and the trend of RC delay in the scaled-down VLSIs are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Cross-Linker-Free <i>N</i>-Isopropylacrylamide Gel Nanospheres (2003)

We have found that the free-radical polymerization of N-isopropylacrylamide (NIPAM) in water initiated by potassium persulfate at temperatures well above the phase transition temperature leads to the formation of gel nanospheres instead of linear chains even in the absence of added cross-linker. These particles, with radii of several hundred nanometers, resemble normal NIPAM microgels in every aspect except that they are characterized by lower solid contents and larger swelling ratios than N,N‘-methylene bis(acrylamide) cross-linked microgels synthesized under similar conditions. Without added cross-linker, the formation of gel nanospheres is attributed to self-cross-linking by chain transfer reaction during and after polymerization.
Throughput optimal energy neutral management for energy harvesting wireless sensor networks (2012)

Energy harvesting techniques have enabled the provisioning of alternative energy sources beside the conventional one which is typically provided by the batteries. Using such harvesting techniques together with proper energy management mechanisms, a Energy Neutral state can be achieved so that desired performance level can be supported perpetually. Existing energy neutral management mechanisms rely on the prediction of the amount of energy that can be harvested in the future. Such mechanisms suffer from the time consuming prediction processes and great fluctuations in the actual implementations. Hence, in this paper we propose real time adaptive energy management policies that enable energy neutral management based solely on observed information in the past instead of predicting the amount of energy that can be harvested in the future. We will show that these policies are throughput optimal in the sense that we are able to ensure that the data queue of each sensor node remain stable when the largest possible data rate is applied under different channel capacity scenarios. We also propose an energy harvest-store(use) method to reduce the energy losses caused by storing energy in non ideal energy buffers. Simulation studies show that our proposed throughput optimal policies and harvest-store(use) method are indeed able to improve the overall throughput as compared to existing schemes.
Snapshot Difference Imaging using Time-of-Flight Sensors (2017)

Computational photography encompasses a diversity of imaging techniques, but one of the core operations performed by many of them is to compute image differences. An intuitive approach to computing such differences is to capture several images sequentially and then process them jointly. Usually, this approach leads to artifacts when recording dynamic scenes. In this paper, we introduce a snapshot difference imaging approach that is directly implemented in the sensor hardware of emerging time-of-flight cameras. With a variety of examples, we demonstrate that the proposed snapshot difference imaging technique is useful for direct-global illumination separation, for direct imaging of spatial and temporal image gradients, for direct depth edge imaging, and more.
Tumor vasculature in young and old hosts: scanning electron microscopy of microcorrosion casts with microangiography, light microscopy and transmission electron microscopy. (1987)

Tumor growth in vivo is dependent upon new blood vessel formation. When B16-F10 melanoma cells are implanted subcutaneously in young (3 mo) and old (24 mo) C57BL/6 mice the rate of growth is dependent on the age of the mice. This study involved a wide range of histological and microscopic techniques but was limited primarily to the initial phase of tumor growth. Stereological point counting from light microscopy (LM) of standard histological sections has been used to yield data regarding blood content. Tumor-bearing mice were perfused through the aorta with a fixation solution and were infused with a low-viscosity radiopaque gel (Microfil) or resin (Mercox). Soft x-rays of the whole animal were used for identifying the feeding vessels to the tumor. Tumors with Microfil were sliced and used for microangiography and light-microscopic observation while those with resin were used to make corrosion casts for scanning electron microscopy (SEM). The different characteristics of the tumor blood vessels in different aged mice were most obvious through SEM of vascular corrosion casts. In comparison with tumors in young mice those of similar size in old hosts had more necrosis, reduced presence of angiogenic features, decreased vessel density, reduced penetration into the tumor, and enhanced tortuosity of the vessel lumen. Transmission electron microscopy (TEM) revealed incompletely developed wall structure of the vessels regardless of host. The above results are consistent with the hypothesis that retarded angiogenesis may be responsible in part for the limited growth of tumors in old hosts.
Isoprene Emission from Velvet Bean Leaves (Interactions among Nitrogen Availability, Growth Photon Flux Density, and Leaf Development) (1994)

Although isoprene synthesis is closely coupled to photosynthesis, both via ATP requirements and carbon substrate availability, control of isoprene emission is not always closely linked to photosynthetic processes. In this study we grew velvet bean (Mucuna sp.) under different levels of photon flux density (PFD) and nitrogen availability in an effort to understand better the degree to which these two processes are linked. As has been observed in past studies, we found that during early leaf ontogeny the onset of positive rates of net photosynthesis precedes that of isoprene emission by 3 to 4 d. Other studies have shown that this lag is correlated with the induction of isoprene synthase activity, indicating that overall control of the process is under control of that enzyme. During leaf senescence, photosynthesis rate and isoprene emission rate declined in parallel, suggesting similar controls over the two processes. This coordinated decline was accelerated when plants were grown with high PFD and high nitrogen availability. The latter effect included declines in the photon yield of photosynthesis, suggesting that an unexplained stress arose during growth under these conditions, triggering a premature decline in photosynthesis and isoprene emission rate. In mature leaves, growth PFD and nitrogen nutrition affected photosynthesis and isoprene emission in qualitatively similar, but quantitatively different, ways. This resulted in a significant shift in the percentage of fixed carbon that was re-emitted as isoprene. In the case of increasing growth PFD, isoprene emission rate was more strongly affected than photosynthesis rate, and more carbon was lost as isoprene. In the case of increasing nitrogen, photosynthesis rate increased more than isoprene emission rate, and leaves containing high amounts of nitrogen lost a lower percentage of their assimilated carbon as isoprene. Taken together, our results demonstrate that, although the general correlation between isoprene emission rate and photosynthesis rate is consistently expressed, there is evidence that both processes are capable of independent responses to plant growth environment.
Six‐year results of treatment with levodopa plus benzerazide in Parkinson's disease (1976)

A combination of levodopa and a peripheral dopa-decarboxylase inhibitor, benzerazide (Ro 4-4602), was studied over a 75-month period of observation in 132 patients with Parkinson's disease. The combined therapeutic approach was without biological toxicity, was well tolerated by 95 percent of patients, and was highly effective: 72 percent of patients improved by more than 50 percent on a functional activity scale and the group as a whole improved on an objective battery by a mean of 46 percent. Neurologic side effects of abnormal involuntary movements, falls, and oscillations in performance were not improved over levodopa used alone. The combined therapy is to be preferred over the use of levodopa alone in the symptomatic management of Parkinson's disease.
Oxidation of 2-keto-4-thiomethylbutyric acid (KTBA) by iron-binding compounds produced by the wood-decaying fungus Gloeophyllum trabeum (1992)

The ability of iron-binding compounds isolated from the brown-rot fungus Gloeophyllum trabeum to carry out one-electron oxidation reactions was established using a model substrate, 2-keto-4-thiomethylbutyric acid (KTBA). The oxidation reaction was monitored by measuring the amount of ethylene produced from the substrate by gas chromatography. The extent of the reaction was found to be influenced by the concentration of the chelators, and by iron and manganese.
HPA Genotyping by PCR‐SSP: Report of 4 Exercises (1999)

Abstract Background and Objectives: Polymerase chain reaction with sequence specific primers (PCR‐SSP) is widely used for the determination of the alleles encoding the human platelet antigens (HPA)‐1 to 5. In order to evaluate and improve performance with this technique, four exercises were organised during 1996‐1998. Materials and Methods: Coded DNA samples were distributed from the National Institute for Biological Standards and Control (NIBSC) as follows: exercise one, 18 samples; two, 12 samples; three, 6 samples, and four, 4 samples. Results: Performance improved over the four exercises following the adoption of a consensus protocol and the re‐design of one primer. The percentage of incorrect results in each exercise was as follows: exercise one, 9%; two 3.2%, three, 0.8%, and four, 0.3% Conclusion: The modified PCR‐SSP protocol is a reliable method for genotyping HPA‐1 to 5 in reference laboratories. DNA‐based HPA genotyping has an important role in platelet immunology and further exercises will be included in the bi‐annual platelet immunology exercises organised by NIBSC.
Laser Micro Machining Of Material Surfaces (1989)

For high precision fabrication of fine structures and for making fine surface textures - a predefined finishing roughness on surfaces - several laser processing techniques are developed at TNO. The laser micro milling technique is applicable to make blind holes, grooves and other programmed shapes. It is a self-adjusting technique applicable e.g. in the manufacturing process of high precision parts for the tool-making industry. The removal of material is achieved by local and repeatedly evaporation of small quantities of material from the workpiece-surface. The ablation rate or milling rate is determined by the laser fluence, the powerdensity and the way the process is programmed. For circular and rectangular shapes recipes are developed, which can be combined for other shapes. A patent on this work, which is carried out in a Brite program* is in preparation. An application of the laser surface texturing technique, by which a variety of finishing roughness reproducable can be achieved, is the making of predefined surface textures on metal moulds for duplicating the texture on plastics products. A laser surfacing technique, which results in glazy layers on metal surfaces, with interesting properties will also be reported. The processes described require lasers and deflection systems which parameters are of good repeatability and constancy; such equipment is available on the market. For the reported work a Nd-YAG laser processing machine is applied while short pulses with a high peak power and high powerdensities on the workpieces surface are required. The technique described can be used for both metallic and ceramic materials.
Real‐time X‐ray powder diffraction investigations on cocoa butter. I. temperature‐dependent crystallization behavior (1996)

Abstract The crystallization behavior of cocoa butter has been investigated by means of real‐time X‐ray powder diffraction. Two procedures have been followed: cooling from 60°C at a constant rate until maximum solidification has taken place; and cooling from 60°C in 2 min to a constant solidification temperature. It appears that all polymorphic forms of cocoa butter, with the exception of the β form, can be formed from liquid. The solidification temperature appears to be the most important crystallization parameter.
On the Production of Semantic and Textured 3D Meshes of Large scale Urban Environments from Mobile Mapping Images and LiDAR scans (2018)

International audience
Linking Covalent Histone Modifications to Epigenetics: The Rigidity and Plasticity of the Marks (2004)

In higher eukaryotes, genetic information, encoded bythe DNA double helix, is organized into a complex chromatin structure with the assistance of histone and nonhistone proteins. As such, chromatin is the physiologicallyrelevant form of eukaryotic genomes. While it is largelyuncontested that different genetic compositions of individual species underlie their unique physiology and finalmorphology, chromatin provides an additional layer fordictating the translation of genetic information intomeaningful biological readouts (for review, see Strahland Allis 2000; Jenuwein and Allis 2001; Felsenfeld andGroudine 2003). Increasing evidence underscores anemerging theme in biology wherein "epigenetic" mechanisms, defined as mechanisms operating outside ofchanges in DNA sequence, govern gene function by creating potentially heritable alternative states of chromatin. DNA methylation and covalent modifications ofhistones, such as methylation, acetylation, and phosphorylation, are thought to contribute to these chromatinstates by serving as a platform that integrates cell differentiation signals and environmental cues as well as regulating the timely release of the appropriate genetic information (Cheung et al. 2000; Schreiber and Bernstein2002). Several of the above histone modifications arereadily reversible through enzymatic means; others arepotentially static (Bannister et al. 2002; see below). Onecentral problem that remains to be resolved is how thesemechanisms are regulated either during normal developmental conditions or under various pathological conditions.EPIGENETIC MECHANISMS UNDERLYINGDIFFERENTIATION DURING MAMMALIA...
Optimal periodic control for spacecraft pointing and attitude determination (1993)

A new approach to autonomous magnetic roll/yaw control of polar-orbiting, nadir-pointing momentum bias spacecraft is considered as the baseline attitude control system for the next Tiros series. It is shown that the roll/yaw dynamics with magnetic control are periodically time varying. An optimal periodic control law is then developed. The control design features a state estimator that estimates attitude, attitude rate, and environmental torque disturbances from Earth sensor and sun sensor measurements; no gyros are needed. The state estimator doubles as a dynamic attitude determination and prediction function. In addition to improved performance, the optimal controller allows a much smaller momentum bias than would otherwise be necessary. Simulation results are given.
Cationic lipid-coated PEI/DNA polyplexes with improved efficiency and reduced cytotoxicity for gene delivery into mesenchymal stem cells (2012)

Noncovalent modification of polyplexes with lysinylated and histidylated cholesterol lipids can simultaneously improve efficiency and reduce the toxicity of gene delivery under serum conditions, showing great promise for genetic modification of bone marrow stem cells.
Understanding Plug-in Test Suites from an Extensibility Perspective (2010)

Plug-in architectures enable developers to build extensible software products. Such products are assembled from plug-ins, and their functionality can be enriched by adding or configuring plug-ins. The plug-ins themselves consist also of multiple plug-ins, and offer dedicated points through which their functionality can be influenced. A well-known example of such an architecture is Eclipse, best known for its use to create a series of extensible IDEs. In order to test systems built from plug-ins developers use extensive automated test suites. Unfortunately, current testing tools offer little insight in which of the many possible combinations of plug-ins and plug-in configurations are actually tested. To remedy this problem, we propose three architectural views that provide an extensibility perspective on plug-in-based systems and their test suites. The views combine static and dynamic information on plug-in dependencies, extension initialization, and extension usage. The views are implemented in ETSE, the Eclipse Plug-in Test Suite Exploration tool. We evaluate the proposed views by analyzing eGit and Mylyn, two open source Eclipse plug-ins.
Effects of Intraperitoneal L-Leucine, L-Isoleucine, L-Valine, and L-Arginine on Milk Fat Depression in Early Lactation Cows (1994)

Eight Holstein cows were assigned following calving to two groups, balanced for parity, using a continuous completely randomized block design. Cows were fed a diet with 13.5% CP and 22.4% ADF from 35 to 55 DIM and then 13.8% CP and 15% ADF from 56 to 92 DIM. Alfalfa grass hay was the forage source, and concentrate mixtures contained primarily corn and soybean meal. Cows were given daily intraperitoneal infusions of a solution of L-Leu (46.1 g, 84.2 mM), L-Ile (31.4 g, 57.3 mM), L-Val (38.3 g, 78.2 mM), and L-Arg (25.0 g, 34.4 mM) or physiological saline following the a.m. milking from 42 through 84 DIM. Infusion of AA significantly increased plasma concentrations of Leu, Ile, Val, and Arg. Effects of AA infusion occurred during the low fiber period. Cows receiving AA maintained daily milk fat yield, increased p.m. milk fat yield, decreased less in p.m. milk fat percentage, and increased daily and p.m. yields of C16 fatty acids in milk. During the posttreatment period, cows previously receiving AA declined in daily milk fat yield, milk fat percentage, and total daily C4 to C16 milk fatty acid yield. Results suggest that the infused AA may have increased de novo synthesis of C16 milk fatty acids.
Modeling the Interaction between Quinolinate and the Receptor for Advanced Glycation End Products (RAGE): Relevance for Early Neuropathological Processes (2015)

The receptor for advanced glycation end products (RAGE) is a pattern-recognition receptor involved in neurodegenerative and inflammatory disorders. RAGE induces cellular signaling upon binding to a variety of ligands. Evidence suggests that RAGE up-regulation is involved in quinolinate (QUIN)-induced toxicity. We investigated the QUIN-induced toxic events associated with early noxious responses, which might be linked to signaling cascades leading to cell death. The extent of early cellular damage caused by this receptor in the rat striatum was characterized by image processing methods. To document the direct interaction between QUIN and RAGE, we determined the binding constant (Kb) of RAGE (VC1 domain) with QUIN through a fluorescence assay. We modeled possible binding sites of QUIN to the VC1 domain for both rat and human RAGE. QUIN was found to bind at multiple sites to the VC1 dimer, each leading to particular mechanistic scenarios for the signaling evoked by QUIN binding, some of which directly alter RAGE oligomerization. This work contributes to the understanding of the phenomenon of RAGE-QUIN recognition, leading to the modulation of RAGE function.
Low-complexity Neural Network-based MIMO Detector using Permuted Diagonal Matrix (2020)

DNN has achieved state-of-the-art performance in MIMO detection problem. However, the deep and large model is hard to deploy to resource constrained platforms. In this work, we propose to provide a sparse DNN model for MIMO detection using permuted diagonal matrices. As a result, our model is with low complexity and doesn't have indexing overhead like other sparsity method. Experiment shows that our method can achieve high sparsity while maintaining the model performance.
Stereocontrolled synthesis of a cis-carbapenem antibiotic (-)-carpetimycin A (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereocontrolled synthesis of a cis-carbapenem antibiotic (-)-carpetimycin ATakamasa Iimori, Yoshio Takahashi, Toshio Izawa, Susumu Kobayashi, and Masaji OhnoCite this: J. Am. Chem. Soc. 1983, 105, 6, 1659–1660Publication Date (Print):March 1, 1983Publication History Published online1 May 2002Published inissue 1 March 1983https://pubs.acs.org/doi/10.1021/ja00344a044https://doi.org/10.1021/ja00344a044research-articleACS PublicationsRequest reuse permissionsArticle Views269Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Caffeic Acid Derivatives Inhibit the Growth of Colon Cancer: Involvement of the PI3-K/Akt and AMPK Signaling Pathways (2014)

CA derivatives are potent anti-cancer agents that augment AMPK activation and promote apoptosis in human CRC cells. The structure of CA derivatives can be used for the rational design of novel inhibitors that target human CRC cells.