gebhart/patrepeval huggingface dataset:
First Example of <i>s</i>-BuLi/(-)-Sparteine-Mediated Chiral Deprotonation of a Piperazine and Proof of the Sense of Induction (2008)

This paper describes the first known example of a (-)-sparteine-mediated asymmetric deprotonation of a piperazine. Reaction of tert-butyl 4-tert-butylpiperazine-1-carboxylate with s-BuLi in the presence of (-)-sparteine at -78 °C and quenching with carbon dioxide proceeded in good yield and with a high degree of selectivity (er = 89:11). X-ray crystal structure analysis on a chiral derivative confirmed the R-enantiomer was the major product. This methodology was employed as a route to an advanced chiral intermediate that was used in parallel synthesis of a range of molecules of medicinal interest. Optimisation of the chemistry used to introduce a tert-butyl group onto a piperazine nitrogen is also described.
Early Eye Development in Vertebrates (2001)

This review provides a synthesis that combines data from classical experimentation and recent advances in our understanding of early eye development. Emphasis is placed on the events that underlie and direct neural retina formation and lens induction. Understanding these events represents a longstanding problem in developmental biology. Early interest can be attributed to the curiosity generated by the relatively frequent occurrence of disorders such as cyclopia and anophthalmia, in which dramatic changes in eye development are readily observed. However, it was the advent of experimental embryology at the turn of the century that transformed curiosity into active investigation. Pioneered by investigators such as Spemann and Adelmann, these embryological manipulations have left a profound legacy. Questions about early eye development first addressed using tissue manipulations remain topical as we try to understand the molecular basis of this process.
Adaptive array processing for multipath fading mitigation via exploitation of filter banks (2001)

We propose subband adaptive array processing for mitigation of both intersymbol interference (ISI) and cochannel interference (CCI) in digital mobile communications. Subband adaptive array processing employs filter banks in a front end to an adaptive array receiver. By decomposing the signals into a set of subband signals, the analysis filters enhance the correlation of multipath rays in each subband. This enhancement is blind in the sense that no a priori knowledge of the temporal characteristics or spatial signatures of arriving signals is required. With the increased coherence, the desired signal can be effectively equalized by subsequent spatial processing. Further, the CCI signals and their multipaths can be suppressed with fewer degrees of freedom. The effects of quadrature mirror filter and discrete Fourier transform filter banks on multipath correlation are delineated.
Declarative Composition and Peer-to-Peer Provisioning of Web Services (2002)

The development of new services through the integration of existing ones has gained a considerable momentum as a means to create and streamline business-to-business collaborations. Unfortunately, as Web services are often autonomous and heterogeneous entities, connecting and coordinating them in order to build integrated services is a delicate and time-consuming task. In this paper, we describe the design and implementation of a system through which existing Web services can be declaratively composed, and the resulting composite services can be executed following a peer-to-peer paradigm, within a dynamic environment. This system provides tools for specifying composite services through statecharts, data conversion rules, and provider selection policies. These specifications are then translated into XML documents that can be interpreted by peer-to-peer inter-connected software components, in order to provision the composite service without requiring a central authority.
Customizable Ligand Exchange for Tailored Surface Property of Noble Metal Nanocrystals (2020)

It is highly desirable, while still challenging, to obtain noble metal nanocrystals with custom capping ligands, because their colloidal synthesis relies on specific capping ligands for the shape control while conventional ligand exchange processes suffer from "the strong replaces the weak" limitation, which greatly hinders their applications. Herein, we report a general and effective ligand exchange approach that can replace the native capping ligands of noble metal nanocrystals with virtually any type of ligands, producing flexibly tailored surface properties. The key is to use diethylamine with conveniently switchable binding affinity to the metal surface as an intermediate ligand. As a strong ligand, it in its original form can effectively remove the native ligands; while protonated, it loses its binding affinity and facilitates the adsorption of new ligands, especially weak ones, onto the metal surface. By this means, the irreversible order in the conventional ligand exchange processes could be overcome. The efficacy of the strategy is demonstrated by mutual exchange of the capping ligands among cetyltrimethylammonium, citrate, polyvinylpyrrolidone, and oleylamine. This novel strategy significantly expands our ability to manipulate the surface property of noble metal nanocrystals and extends their applicability to a wide range of fields, particularly biomedical applications.
The impact of RAC audits on US hospitals. (2013)

The Medicare Prescription Drug, Improvement, and Modernization Act of 2003 (MMA) authorized a three-year demonstration program using recovery audit contractors (RACs) to identify and correct improper payments in the Medicare Fee-For-Service program. More recently, Section 6411 of the Affordable Care Act (ACA) expanded the RAC program to include the Medicaid program. This shows the Cent ers for Medicare & Medicaid Services (CMS) believe RAC audits are a cost-effective method to ensure health care providers are paid correctly and thereby protect the Medicare Trust Fund. RAC audits are highly complex and require significant manpower to handle the large volume of requests received during a short period of time. Additionally, the RAC audit appeal process is complicated and requires a high level of technical expertise. The demonstration project found that RAC audits resulted in sizeable amounts of overpayments collected ("take-backs") from many providers. This research study assesses the potential impact of the RAC audit program on US acute care hospitals. Data obtained from CMS show that RAC overpayments collected for FY 2010 were $75.4 million, increased to $797.4 million in FY 2011, and increased to $986.2 million in the first six months of FY 2012. According to the American Hospital Association (AHA) RACTrac audit survey, the vast majority of these collections represent complex denials where hospitals are required to provide medical record documents in support of their billed claims. This study found that the RAC audit program collections are increasing significantly over time. As a result, these collections are having a significant negative impact on the profitability of US hospitals.
Improving the bulk data transfer experience (2008)

Scientific computations and collaborations increasingly rely on the network to provide high-speed data transfer, dissemination of results, access to instruments, support for computational steering, etc. The Energy Sciences Network is establishing a science data network to provide user driven bandwidth allocation. In a shared network environment, some reservations may not be granted due to the lack of available bandwidth on any single path. In many cases, the available bandwidth across multiple paths would be sufficient to grant the reservation. In this paper we investigate how to utilise the available bandwidth across multiple paths in the case of bulk data transfer.
Chimeric Antigen Receptor T-Cell Therapy: Reach to Solid Tumor Experience (2019)

Chimeric antigen receptor (CAR) modified T-cell therapy, a unique platform technology highlighting precision medicine through utilization of molecular biology and cell-based therapeutics has shown unprecedented rates in patients with hematological malignancies such as acute lymphocyte leukemia, non-Hodgkin's lymphoma and multiple myeloma (MM). With the approval of CD19-targeted CAR T-cells by the Food and Drug Administration in acute lymphoblastic leukemia (ALL) and NHL, this technology is positioned for aggressive expansion to combination therapeutic opportunities and proof of principle towards utility in other malignant disorders. However, despite the impressive results seen with hematological malignancies, CAR T-cells have shown limited efficacy in solid tumors with several unsuccessful preclinical studies. Regardless, these attempts have provided us with a better understanding of the imminent challenges specific to solid tumors even if they have not so far led to expanded clinical treatment opportunities outside ALL/NHL/MM. This review summarizes our current understanding of CAR T-cell mechanism of action, while presenting the major limitations of CAR T-cell derived treatments in solid tumors. We further discuss recent findings and present new potential strategies to overcome the challenges facing solid tumor targeting by CAR T-cell platforms.
Non-uniform sampling strategies for continuous correction based trajectory estimation (2016)

Sliding window estimation is widely used for online simultaneous localization and mapping. While increasing the sliding window size generally yields improved accuracy, it also comes at an increase in computational cost. In order to reduce this cost, we propose smarter non-uniform sampling of the trajectory representation over the sliding window. This non-uniform temporal resolution is possible with continuous-time representations that allow freely adjustable knots location. Four strategies for selecting the knots location are presented and evaluated based on a real data laser-odometry SLAM problem. The results clearly show that non-uniform distributions of knots can be superior to uniform distribution in terms of accuracy per computation time.
Additive noise properties of active matrix flat‐panel imagers (2000)

A detailed theoretical and empirical investigation of additive noise for indirect detection, active matrix flat-panel imagers (AMFPIs) has been performed. Such imagers comprise a pixelated array, incorporating photodiodes and thin-film transistors (TFTs), and an associated electronic acquisition system. A theoretical model of additive noise, defined as the noise of an imaging system in the absence of radiation, has been developed. This model is based upon an equivalent-noise-circuit representation of an AMFPI. The model contains a number of uncorrelated noise components which have been designated as pixel noise, data line thermal noise, externally coupled noise, preamplifier noise and digitization noise. Pixel noise is further divided into the following components: TFT thermal noise, shot and 1/f noise associated with the TFT and photodiode leakage currents, and TFT transient noise. Measurements of various additive noise components were carried out on a prototype imaging system based on a 508 microm pitch, 26 x 26 cm2 array. Other measurements were performed in the absence of the array, involving discrete components connected to the preamplifier input. Overall, model predictions of total additive noise as well as of pixel, preamplifier, and data line thermal noise components were in agreement with results of their measured counterparts. For the imaging system examined, the model predicts that pixel noise is dominated by shot and 1/f noise components of the photodiode and TFT at frame times above approximately 1 s. As frame time decreases, pixel noise is increasingly dominated by TFT thermal noise. Under these conditions, the reasonable degree of agreement observed between measurements and model predictions provides strong evidence that the role of TFT thermal noise has been properly incorporated into the model. Finally, the role of the resistance and capacitance of array data lines in the model was investigated using discrete component circuits at the preamplifier input. Measurements of preamplifier noise and data line thermal noise components as a function of input capacitance and resistance were found to be in reasonable agreement with model predictions.
Residence Time of Receptor−Ligand Complexes and Its Effect on Biological Function (2008)

The formation and duration of binary receptor-ligand complexes are fundamental to many physiologic processes. Most often, the effectiveness of interaction between a receptor and its ligand is quantified in terms of closed system, equilibrium affinity measurements, such as IC50 and Kd. In the context of in vivo biology, however, the extent and duration of responses to receptor-ligand interactions depend greatly on the time period over which the ligand is in residence on its receptor. Here we define receptor-ligand complex residence time in quantitative terms and describe its significance to biological function. Examples of the importance of residence time are presented for natural ligands of different receptor types. The impact of residence time on the optimization of potential ligands as drugs for human medicine is also described.
High value implanted resistors for microcircuits (1969)

Boron ion implantation has been used to fabricate high sheet resistance p-type junction resistors in silicon substrates. Thermally grown SiO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> and conventional photolithography were employed to define the resistor geometries. Ion doses in the range 0.5 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">13</sup> to 10 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">13</sup> ions/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> with energies ranging from 30 to 55 keV followed by anneal at 950°C were used. The temperature coefficient of resistance (TCR), voltage coefficient of resistance (VCR), junction Characteristics, and noise level of these resistors have been studied for sheet resistances ρ <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</inf> , from 0.8 to 11 kΩ/square. Over this range of sheet resistances the TCR increases smoothly from approximately 800 to 4000 PPM/°C with the lower TCR corresponding to the lower sheet resistance. For 3 kΩ square implanted resistors, the variation of resistance with temperature closely matches that found for a standard boron base and resistor (B&R) diffusion having a sheet resistance of 140 Ω/square. The junction leakage and the noise level of the implanted resistors can be made comparable to that obtained for diffused resistors. The implanted resistor exhibits a positive VCR, which increases with increasing sheet resistance as a result of depletion-layer pinch-off action from the substrate. Details of the implant conditions and process control are discussed. Experimental results demonstrating the compatibility of the resistor implantation process with microcircuits using low current, high β diffused bipolar transistors are presented.
Sentiment Analysis with Word Embedding (2018)

The basic task of sentiment analysis is to determine the sentiment polarity (positivity, neutrality or negativity) of a piece text. The traditional bag-of-words models deficiencies affect the accuracy of sentiment classifications. The purpose of this study is to improve the accuracy of the sentiment classification by employing the concept of word embedding. This study uses Word2Vec to produce high-dimensional word vectors that learn contextual information of words. The resulting word vectors are used to train machine learning algorithms in the form of classifiers for sentiment classification. Our experiments on real world datasets shows that the use of word embedding improves the accuracy of sentiments classification.
<i>Saccharomyces cerevisiae</i> glucose signalling regulator Mth1p regulates the organellar Na+/H+ exchanger Nhx1p (2010)

Organelle-localized NHEs (Na+/H+ exchangers) are found in cells from yeast to humans and contribute to organellar pH regulation by exporting H+ from the lumen to the cytosol coupled to an H+ gradient established by vacuolar H+-ATPase. The mechanisms underlying the regulation of organellar NHEs are largely unknown. In the present study, a yeast two-hybrid assay identified Mth1p as a new binding protein for Nhx1p, an organellar NHE in Saccharomyces cerevisiae. It was shown by an in vitro pull-down assay that Mth1p bound to the hydrophilic C-terminal half of Nhx1p, especially to the central portion of this region. Mth1p is known to bind to the cytoplasmic domain of the glucose sensor Snf3p/Rgt2p and also functions as a negative transcriptional regulator. Mth1p was expressed in cells grown in a medium containing galactose, but was lost (possibly degraded) when cells were grown in medium containing glucose as the sole carbon source. Deletion of the MTH1 gene increased cell growth compared with the wild-type when cells were grown in a medium containing galactose and with hygromycin or at an acidic pH. This resistance to hygromycin or acidic conditions was not observed for cells grown with glucose as the sole carbon source. Gene knockout of NHX1 increased the sensitivity to hygromycin and acidic pH. The increased resistance to hygromycin was reproduced by truncation of the Mth1p-binding region in Nhx1p. These results implicate Mth1p as a novel regulator of Nhx1p that responds to specific extracellular carbon sources.
Notch-Expanded Murine Hematopoietic Stem and Progenitor Cells Mitigate Death from Lethal Radiation and Convey Immune Tolerance in Mismatched Recipients (2016)

The hematopoietic syndrome of acute radiation syndrome (h-ARS) is characterized by severe bone marrow aplasia, resulting in a significant risk for bleeding, infections, and death. To date, clinical management of h-ARS is limited to supportive care dictated by the level of radiation exposure, with a high incidence of mortality in those exposed to high radiation doses. The ideal therapeutic agent would be an immediately available, easily distributable single-agent therapy capable of rapid in vivo hematopoietic reconstitution until recovery of autologous hematopoiesis occurs. Using a murine model of h-ARS, we herein demonstrate that infusion of ex vivo expanded murine hematopoietic stem and progenitor cells (HSPCs) into major histocompatibility complex mismatched recipient mice exposed to a lethal dose of ionizing radiation (IR) led to rapid myeloid recovery and improved survival. Survival benefit was significant in a dose-dependent manner even when infusion of the expanded cell therapy was delayed 3 days after lethal IR exposure. Most surviving mice (80%) demonstrated long-term in vivo persistence of donor T cells at low levels, and none had evidence of graft versus host disease. Furthermore, survival of donor-derived skin grafts was significantly prolonged in recipients rescued from h-ARS by infusion of the mismatched expanded cell product. These findings provide evidence that ex vivo expanded mismatched HSPCs can provide rapid, high-level hematopoietic reconstitution, mitigate IR-induced mortality, and convey donor-specific immune tolerance in a murine h-ARS model. Stem Cells Translational Medicine 2017;6:566-575.
Taxol assembles tubulin in the absence of exogenous guanosine 5'-triphosphate or microtubule-associated proteins (1981)

Taxol increases the rate and extent of microtubule assembly in vitro and stabilizes microtubules in vitro and in cells [Schiff, P. B., Fant, J., & Horwitz, S. B. (1979) Nature (London) 277, 665-667; Schiff, P. B., & Horwitz, S. B. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 1561-1565]. We report herein that taxol has the ability to promote microtubule assembly in the absence of microtubule-associated proteins, rings, and added guanosine 5'-triphosphate (GTP or organic buffer. The drug enhances additional microtubule assembly when added to microtubules at apparent steady state. This additional assembly can be attributed to both elongation of existing microtubules and spontaneous nucleation of new microtubules. Taxol-treated microtubules have depressed dissociation reactions as determined by dilution experiments. The drug does not inhibit the binding of GTP or the hydrolysis of GTP or guanosine 5'-diphosphate (GDP) in our microtubule protein preparations. Taxol does not competitively inhibit the binding of colchicine to tubulin.
Additional Purification of Some Salts by Using Silica Gel Modified with Calmagit as a Sorbent (1992)

Abstract Sorption of 35 metal ions from aqueous solutions in the pH range between 1 and 9 by silica gel impregnated with a mixture of Aliquat 336 and Calmagit was investigated. The retained metals can be eluted with dilute solutions of hydrochloric or perchloric acids. The sorbent was used for purification of some potassium, sodium, and ammonium salts from trace amounts of zinc, lead, cadmium, and copper. The effectiveness of purification was confirmed by anodic stripping voltammetry. Some metal ion mixtures have also been separated by using column extraction chromatography and stepwise elution with perchloric acid solutions.
Quality management and influential factors for the detection of single metastatic cancer cells by reverse transcriptase polymerase chain reaction. (1997)

The sensitive and specific detection of micrometastasis holds great promise for earlier staging of cancer patients. By amplification of tissue-specific gene expression, the reverse transcriptase polymerase chain reaction (rtPCR) readily detects single tumour cells in different tissues. An increasing number of rtPCR assays with possible relevance for routine laboratory diagnostic procedures is currently being reported in the literature. Interestingly, when used in the clinical setting, assays for the same target mRNA perform very differently, despite comparable sensitivities and specificities in-vitro. Using rtPCRs specific for carcinoembryonic antigen (CEA), prostate-specific antigen (PSA) and cytokeratin 18 (CK 18), we have started to systematically investigate, both experimentally and in clinical specimens, a number of factors that contribute to the varying and seemingly implausible test results. Here we have concentrated on sample collection modalities, assay stability and test reproducibility at the sensitivity limit. Our results demonstrate in detail that, at the maximum sensitivity required for micrometastasis detection, preanalytical and statistical influences increasingly become important for the consistency of the assay results. We conclude that the prerequisite for translating the results from highly sensitive and specific rtPCR assays into clinically relevant data is the thorough definition of assay procedures and the number of tests performed on a sample. Addressing questions of standardization and quality control management is a central aspect yet to be emphasized in assay development and application of routine laboratory rtPCR tests in oncology.
Computational techniques for vertex partitioning of graphs (1990)

A powerful vertex-partitioning algorithm is developed and applied for vertex partitioning of graphs of chemical and spectroscopic interest. The codes developed on the basis of these algorithms are tested and compared for performance with other methods based on the Morgan algorithm and the principal eigenvector algorithm based on the Givens-Householder method. The newly developed algorithm and codes appear to be more powerful than the Morgan and the principal eigenvector algorithms for vertex partitioning of graphs.