gebhart/patrepeval huggingface dataset:
The activation of murine macrophages and natural killer cells by the partially thiolated double stranded RNA poly(I)-mercapto poly(C). (1996)

Partially thiolated analogs of the biological response modifier poly I.poly C (pI.pC) were synthesized. Each of these analogs (pI.MPC) contained a partially thiolated polycytidylate (MPC) strand containing either 1.2%, 4.6%, or 17% 5-mercaptocytosine (%SH) randomly introduced throughout the polynucleotide. The ability of these double stranded RNAs (dsRNAs) to activate murine peritoneal macrophages in vitro and augment natural killer (NK) cell activity in mice following intraperitoneal (i.p.) administration was determined. Macrophages were treated in vitro for 24 hours with pI.pC, or pI.MPC, washed, and then overlayed with exponentially growing L1210 leukemia cells at an effector to target (E:T) ratio of 25:1. The cytostatic effect of the macrophages on the L1210 cells was determined by 3H.thymidine pulse labelling. The rank order of potency for macrophage activation was determined to be: pI.pC>pI.MPC(1.2% SH)>pI.MPC(4.6% SH)pI.MPC(17% SH). Twenty hours following i.p. administration of 5 mg/kg of each pI.MPC analog, splenic NK cell activity was assessed in a standard 51Cr release assay using the murine tumor target cell line YAC- 1. The rank order of potency observed for NK cell activation was determined to be; pI.pCpI MPC(1.2% SH)>pI.MPC(4.6% SH)>pI.MPC(17% SH). These dsRNAs activated NK cells in a dose dependent manner. The efficacy and time course for NK cell activation following i.p. administration of pI.MPC (1.2% SH) at a dose of 10 mg/kg was directly compared to an equivalent 10 mg/kg i.p. dose of pI.pC. NK cell activation took place within three hours following treatment with pI-pC whereas the onset of NK cell activation by pI.MPC (1.2%) occurred between 8 and 20 hours post treatment. NK cell activity steadily declined from 24 to 50 hours post treatment at which time the NK activity in both treatment groups was similar. There was a significant correlation between the immunostimulatory potency of these dsRNAs and their experimentally determined melting temperatures (r2 = 0.88) and percent hyperchromicity upon thermal denaturation (r2 = 0.99). At the lower %SH, pI.MPC retains most of the immunostimulatory activities of p1.pC and may serve as a useful and potent biological response modifier.
SCAI expert consensus statement for renal artery stenting appropriate use (2014)

The pathophysiology of atherosclerotic renal artery stenosis (RAS) includes activation of the renin-angiotensin-aldosterone axis with resultant renovascular hypertension. Renal artery stenting has emerged as the primary revascularization strategy in most patients with hemodynamically significant atherosclerotic RAS. Despite the frequency with which hemodynamically significant RAS is observed and high rates of technical success of renal artery stenting, there remains considerable debate among experts regarding the role of medical therapy versus revascularization for renovascular hypertension. Modern, prospective, multicenter registries continue to demonstrate improvement in systolic and diastolic blood pressure with excellent safety profiles in patients with RAS. Modern randomized, controlled clinical trials of optimal medical therapy versus renal stenting particularly designed to demonstrate preservation in renal function after renal artery stenting have demonstrated limited benefit. However, these trials frequently excluded patients that may benefit from renal artery stenting. This document was developed to guide physicians in the modern practical application of renal stenting, to highlight the current limitations in the peer-reviewed literature, to suggest best-practices in the performance of renal stenting and to identify opportunities to advance the field.
High-Power Tunable Terahertz Sources Based on Parametric Processes and Applications (2007)

We summarize our progress made on developing widely tunable monochromatic terahertz sources. They have been implemented based on difference-frequency generation (DFG) in GaSe, ZnGeP, and GaP crystals. Using a GaSe crystal, the output wavelength was tuned continuously in the range of 66.5 to 5664 m (from 150 to 1.77 cm with the peak power reaching 389 W. Such a high peak power corresponds to a conversion efficiency of 0.1% (a photon conversion efficiency of 19%). A further optimization on the terahertz beam parameter may result in higher output powers and conversion efficiencies. Our experimental results indicate that within the range of 100-250 mum, the output peak powers were higher than 100 W. On the other hand, based on DFG in a ZnGeP crystal, the output wavelength was generated to be tunable in the ranges of 83.1-1642 mum and 80.2-1416 mum for two phase-matching configurations. The output power reached 134 W. Using a GaP crystal, the output wavelength was tuned in the range of 71.1-2830 m, whereas the highest peak power reached 15.6 W. GaP offers an advantage for tuning the output wavelength compared with GaSe and ZnGeP since crystal rotation is no longer required. Instead, one just needs to tune the wavelength of one mixing beam within a narrow bandwidth of 15.3 nm. Based on power scaling, a shoe box-sized tunable terahertz source is feasible. We also review our recent results obtained following the investigation of backward DFG and feasibility studies on backward parametric oscillation. The terahertz radiations produced by DFG have pulse durations of about 5 ns, wide tuning ranges, and narrow linewidths, which are quite different from the broadband terahertz pulses. We also describe a few important applications that were realized by taking advantage of the wide tuning range and narrow linewidths of terahertz pulses such as chemical sensing and differentiation of isotopic variants by measuring the rotational spectra of gases and terahertz imaging.
OpenDial: A Toolkit for Developing Spoken Dialogue Systems with Probabilistic Rules (2016)

We present a new release of OpenDial, an open-source toolkit for building and evaluating spoken dialogue systems. The toolkit relies on an information-state architecture where the dialogue state is represented as a Bayesian network and acts as a shared memory for all system modules. The domain models are specified via probabilistic rules encoded in XML. Open-Dial has been deployed in several application domains such as human-robot interaction, intelligent tutoring systems and multi-modal in-car driver assistants.
Pistoning assessment in lower limb prosthetic sockets (2012)

The results of this review reveal that further research is needed to develop and evaluate easy, accurate and safe methods of measuring pistoning. Future studies should provide a gold standard for the acceptable range of pistoning in a prosthetic socket.
Unloading-reloading uniaxial compressibility of deep reservoirs by marker measurements (2003)

The radioactive marker technique appears to be a promising tool for the in situ measurement of the uniaxial vertical compressibility cM of deep gas/oil reservoirs. A constitutive model for cM,unload in the Northern Adriatic sedimentary basin in unloading-reloading (II cycle) conditions is derived. This relies on marker measurements obtained from an instrumented borehole located in depleted gas reservoirs that had experienced a pore pressure recovery over the period 1997-1999. Record analysis indicates that cM,unload is on the order of 5-6·10-5 MPa-1 over the effective stress interval 35 ≤ σz ≤ 65 MPa, i.e. for a burial depth between 3200 and 5800 m in undisturbed conditions. The ratio of virgin loading cM,load to cM,unload varies between 1.5 and 3 with an average value equal to 2.2. The cM,unload constitutive law derived for rock expansions is implemented into a coupled finite element consolidation model in an attempt to reproduce, also quantitatively, the vertical deformation recorded i the marker borehoole after field abandonment.
Lactoperoxidase from human colostrum (1989)

The present study has confirmed that human colostrum contains a lactoperoxidase (EC 1.11.1.7) [Langbakk & Flatmark (1984) FEBS Lett. 174, 300-303], which represents about 0.004% of the total protein in crude colostrum. An apparent 32-fold purification of the enzyme was obtained by a multistep procedure, as modified from that of the bovine enzyme, with a recovery of about 7%. By use of chromatography on an immunoaffinity column (directed against bovine lactoperoxidase B), an apparent 1450-fold purification was obtained in a single step, with a recovery of 21%. The enzyme behaved as a glycoprotein (binding to concanavalin A-Sepharose), and revealed spectral properties (Soret peak at 412 nm) and an Mr (80,000) similar to those of the bovine enzyme.
Rolling-circle replication of viroids, viroid-like satellite RNAs and hepatitis delta virus: Variations on a theme (2011)

Viroids and viroid-like satellite RNAs from plants, and the human hepatitis delta virus (HDV) RNA share some properties that include small size, circularity and replication through a rolling-circle mechanism. Replication occurs in different cell compartments (nucleus, chloroplast and membrane-associated cytoplasmatic vesicles) and has three steps: RNA polymerization, cleavage and ligation. The first step generates oligomeric RNAs that result from the reiterative transcription of the circular templates of one or both polarities, and is catalyzed by either the RNA-dependent RNA polymerase of the helper virus on which viroid-like satellite RNAs are functionally dependent, or by host DNA-dependent RNA polymerases that, remarkably, viroids and HDV redirect to transcribe RNA templates. Cleavage is mediated by host enzymes in certain viroids and viroid-like satellite RNAs, while in others and in HDV is mediated by cis-acting ribozymes of three classes. Ligation appears to be catalyzed mainly by host enzymes. Replication most likely also involves many other non-catalytic proteins of host origin and, in HDV, the single virus-encoded protein.
Molecular Mechanism for Lipid Flip-Flops (2007)

Transmembrane lipid translocation (flip-flop) processes are involved in a variety of properties and functions of cell membranes, such as membrane asymmetry and programmed cell death. Yet, flip-flops are one of the least understood dynamical processes in membranes. In this work, we elucidate the molecular mechanism of pore-mediated transmembrane lipid translocation (flip-flop) acquired from extensive atomistic molecular dynamics simulations. On the basis of 50 successful flip-flop events resolved in atomic detail, we demonstrate that lipid flip-flops may spontaneously occur in protein-free phospholipid membranes under physiological conditions through transient water pores on a time scale of tens of nanoseconds. While the formation of a water pore is induced here by a transmembrane ion density gradient, the particular way by which the pore is formed is irrelevant for the reported flip-flop mechanism: the appearance of a transient pore (defect) in the membrane inevitably leads to diffusive translocation of lipids through the pore, which is driven by thermal fluctuations. Our findings strongly support the idea that the formation of membrane defects in terms of water pores is the rate-limiting step in the process of transmembrane lipid flip-flop, which, on average, requires several hours. The findings are consistent with available experimental and computational data and provide a view to interpret experimental observations. For example, the simulation results provide a molecular-level explanation in terms of pores for the experimentally observed fact that the exposure of lipid membranes to electric field pulses considerably reduces the time required for lipid flip-flops.
Nanoparticles That Display Short Collagen-Related Peptides. Potent Stimulation of Human Platelet Aggregation by Triple Helical Motifs (2007)

Collagen plays a key role in the activation and adhesion of blood platelets via their cell-surface receptors. Normally, collagen-related peptides (CRPs), even one as long as a 30-mer (10 Gly-Pro-Hyp (GPO) repeats), are unable to effectively express collagen's platelet-activating behavior. We attached two short CRPs, AcHN-(Gly-Pro-Hyp)nGly-OH with n = 5 (1) and n = 10 (2), via the C-terminus to amino-functionalized latex nanoparticles to create a multimeric display of triple helical motifs. These nanomaterials were characterized by dynamic light scattering and environmental scanning electron microscopy. The nanoparticles bearing the 31-mer CRP sequence, 2, but not the 16-mer sequence, 1, effectively induced the aggregation of human platelets, with a potency level approaching that of native type I collagen. Our results highlight the importance of presenting triple helical CRP motifs of sufficient length on a suitable scaffold in order to stimulate platelets.
Impact of the elevation of biochemical markers of myocardial damage on long-term mortality after percutaneous coronary intervention: results of the CK-MB and PCI study (2005)

Post-procedural elevations of CK-MB, but not cTnI, influence 2-year mortality.
A Phosphotyrosine-dependent Protein Interaction Screen Reveals a Role for Phosphorylation of Caveolin-1 on Tyrosine 14 (2002)

Caveolin-1 is a substrate for nonreceptor tyrosine kinases including Src, Fyn, and Abl. To investigate the function of caveolin-1 phosphorylation, we modified the Gal4-based yeast two-hybrid system to screen for phosphorylation-dependent protein interactions. A cDNA library was screened using the N terminus of caveolin-1 as bait in a yeast strain expressing the catalytic domain of Abl. We identified two proteins in this screen that interact with caveolin-1 in a phosphorylation-dependent manner: tumor necrosis factor-alpha receptor-associated factor 2 (TRAF2) and C-terminal Src kinase (Csk). TRAF2 bound to nonphosphorylated caveolin-1, but this association was increased 3-fold by phosphorylation. In contrast, association of Csk with caveolin-1 was completely dependent on phosphorylation of caveolin-1, both for fusion proteins in yeast (>35-fold difference in affinity) and for endogenous proteins in tissue culture cells. Our data suggest that phosphorylation of caveolin-1 leads to Csk translocation into caveolae. This may induce a feedback loop that leads to inactivation of the Src family kinases that are highly enriched in caveolae.
Food Carbohydrates (2005)

Unique in its broad range of coverage, Food Carbohydrates: Chemistry, Physical Properties and Applications is a comprehensive, single-source reference on the science of food carbohydrates. This text goes beyond explaining the basics of food carbohydrates by emphasizing principles and techniques and their practical application in quality control, pr
Identification of in vivo target RNA sequences bound by thymidylate synthase (1996)

We developed an immunoprecipitation-RNA-random PCR (rPCR) method to isolate cellular RNA sequences that bind to the folate-dependent enzyme thymidylate synthase (TS). Using this approach, nine different cellular RNAs that formed a ribonucleoprotein (RNP) complex with thymidylate synthase (TS) in human colon cancer cells were identified. RNA binding experiments revealed that seven of these RNAs bound TS with relatively high affinity (IC50 values ranging from 1.5 to 6 nM). One of the RNAs was shown to encode the interferon (IFN)-induced 15 kDa protein. Western immunoblot analyses demonstrated that the level of IFN-induced 15 kDa protein was significantly decreased in human colon cancer H630-R10 cells compared with parent H630 cells. While the level of IFN-induced 15 kDa mRNA expression was the same in parent and TS-overexpressing cell lines, the level of IFN-induced 15 kDa RNA bound to TS in the form of a RNP complex was markedly higher in H630-R10 cells relative to parent H630 cells. These studies begin to define a number of cellular target RNA sequences with which TS interacts and suggest that these TS protein-cellular RNA interactions may have a biological role.
An immunomodulatory polysaccharide-rich substance from the fruit juice of Morinda citrifolia (noni) with antitumour activity (1999)

The fruit juice of Morinda citrifolia (noni) contains a polysaccharide-rich substance (noni-ppt) with antitumour activity in the Lewis lung (LLC) peritoneal carcinomatosis model. Therapeutic administration of noni-ppt significantly enhanced the duration of survival of inbred syngeneic LLC tumour bearing mice. It did not exert significant cytotoxic effects in an adapted culture of LLC cells, LLC1, but could activate peritoneal exudate cells (PEC) to impart profound toxicity when co-cultured with the tumour cells. This suggested the possibility that noni-ppt may suppress tumour growth through activation of the host immune system. Concomitant treatment with the immunosuppressive agent, 2-chloroadenosine (C1-Ade) or cyclosporin (cys-A) diminished its activity, thereby substantiating an immunomodulatory mechanism. Noni-ppt was also capable of stimulating the release of several mediators from murine effector cells, including tumour necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), IL-10, IL-12 p70, interferon-gamma (IFN-gamma) and nitric oxide (NO), but had no effect on IL-2 and suppressed IL-4 release. Improved survival time and curative effects occurred when noni-ppt was combined with sub-optimal doses of the standard chemotherapeutic agents, adriamycin (Adria), cisplatin (CDDP), 5-fluorouracil (5-FU), and vincristine (VCR), suggesting important clinical applications of noni-ppt as a supplemental agent in cancer treatment.
Reducing Length of Stay Through Rapid Clinical Redesign (2002)

The SETON Healthcare Network in Austin, TX, significantly reduced length of stay in six diagnostic-related groupings by successfully combining medical management activities and cost-savings targets. This article describes how organizational infrastructure, leadership, clinical redesign, and risk-adjusted data were combined to drive performance improvements and create enthusiasm for change.
A Modified Maxwell and a Nonexponential Model for Characterization of the Stress Relaxation of Agar and Alginate Gels (1989)

ABSTRACT Compressive stress relaxation curves of agar and alginate gels of different gum concentration (1–3% and 0.5–2%, respectively) were fitted by a two parameter nonexponential empirical model and a three term modified Maxwell model with two fixed relaxation times (10 and 100s). The asymptotic portion of the residual (unrelaxed) stress calculated by the two models for each gel had a similar magnitude, and therefore could serve as an objective measure of the gels degree of solidity. The coefficients of the modified Maxwell model provided a simple but meaningful means to compare, in quantitative terms, the differences in the relaxation time spectra that were associated with the gels' stiffness and strength.
A Case Report of Benign Chondroblastoma of the Patella (1973)

The authors experienced a case of benign chondroblastoma involving the patella. To our knowledge this is the third recorded benign chondroblastoma in this site.The patient, a seventeen-year-old boy, was admitted with complaint of pain in the patella.Roentgenograms revealed a multiloculated radiolucent area in the patella without cortical destruction.Microscopically, there were polygonal or rounded stromal cells and small giant cells. There were also several areas of chondroid matrix which appeared to be derived from young chondroblasts.Curettage and bone grafting was carried out and no evidence of recurrence ocurred clinically six months after operation.
What??s in a Name? (1987)

Jones, Mary R.N., C.C.N.S.A.P.C.; Politio, Mary Z. C.R.N.P.; Richmond, Mike C.R.N.P.; Dirubbo, Nancy E. R.N., F.N.P.C. Author Information
Reliability Improvements of Dichromated Gelatin Color Filter for TFT‐LCDs (1991)

Materials and processing technologies for a high quality color filter consisting of ammonium‐dichromated gelatin (ADG) were studied. To achieve adequate characteristics of color reproducibility, the fixing process using tannic acid and potassium antimonyl tartrate and the thermal stability were investigated. The following results were attained: (i). It was clarified that the fixing process played two roles such as the strengthening of the binding force between gelatin and dye molecule, and prevention of a reaction between the remaining dyeing sites in gelatin and dye molecules. Careful control of the fixing process has improved the disappearance of discoloration of ADG patterns during the next dyeing process. (ii). The fading of the color layer was closely associated with the chemical structure of the dye. Anthraquinone dyes reduced the fading due to water absorption and heating of the film. Considering the above results, a reliable, reproducible, and high quality color filter for liquid crystal displays has been realized.
Binding of<i>Plasmodium</i>merozoite proteins RON2 and AMA1 triggers commitment to invasion (2011)

The commitment of Plasmodium merozoites to invade red blood cells (RBCs) is marked by the formation of a junction between the merozoite and the RBC and the coordinated induction of the parasitophorous vacuole. Despite its importance, the molecular events underlying the parasite's commitment to invasion are not well understood. Here we show that the interaction of two parasite proteins, RON2 and AMA1, known to be critical for invasion, is essential to trigger junction formation. Using antibodies (Abs) that bind near the hydrophobic pocket of AMA1 and AMA1 mutated in the pocket, we identified RON2's binding site on AMA1. Abs specific for the AMA1 pocket blocked junction formation and the induction of the parasitophorous vacuole. We also identified the critical residues in the RON2 peptide (previously shown to bind AMA1) that are required for binding to the AMA1 pocket, namely, two conserved, disulfide-linked cysteines. The RON2 peptide blocked junction formation but, unlike the AMA1-specific Ab, did not block formation of the parasitophorous vacuole, indicating that formation of the junction and parasitophorous vacuole are molecularly distinct steps in the invasion process. Collectively, these results identify the binding of RON2 to the hydrophobic pocket of AMA1 as the step that commits Plasmodium merozoites to RBC invasion and point to RON2 as a potential vaccine candidate.
Frame-Size Adaptive MAC Protocol in High-Rate Wireless Personal Area Networks (2006)

In this letter, we propose a frame-size adaptive MAC protocol for high rate wireless personal area networks (WPANs). In the proposed scheme, during communication, frame error rate is periodically reported to a transmitting device and the frame size is changed according to the measured results. Thus, the channel can be more effectively utilized by adapting to variable radio conditions. Analytical results show that this scheme achieves a much higher throughput than a non-frame-size adaptive media access control protocol in high-rate WPANs.
The Efficacy of Newer Antidepressants in the Treatment of Chronic Pain: A Review of Current Literature (2000)

Tricyclic antidepressants have been extensively studied and frequently used in the treatment of various chronic pain syndromes. Newer antidepressants, namely fluoxetine, sertraline, paroxetine, fluvoxamine, citalopram, trazodone, nefazodone, bupropion, mirtazapine, and venlafaxine, have also been considered for this indication, although they have been less extensively studied. This article reviews the available publications, including placebo-controlled trials, other outcome studies, and case reports, pertaining to the use of these medications for chronic pain. Although some of these newer compounds may be effective for specific types of pain, making generalizations regarding their use as analgesics is difficult, given the limitations of existing data. Additional observations based on the data are presented in the hope that they may help to guide further research and clinical use.