gebhart/patrepeval huggingface dataset:
Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase (2013)

Human neutrophil elastase (HNE) is a serine protease implicated in the pathogenesis of acute and chronic inflammatory disease. Here a series of, internally quenched, single fluorophore fluorescent reporters were synthesised that allowed the rapid, highly specific and sensitive analysis of HNE activity over closely related proteases.
Symbolic verification of executable control specifications (2003)

Stateflow/sup TM/ is a MATLAB/Simulink toolbox that supports the development of executable specifications for discrete-state functions. It also supports general flowcharting of program functions. This paper describes a MATLAB program, sf2smv 2.0, that generates input for a symbolic model checking program, SMV, to verify properties of Stateflow/sup TM/ diagrams in Simulink. The SMV modules are constructed to reflect precisely the execution semantics in the simulation of Stateflow/sup TM/ diagrams. This extends previous work that created verification files that reflected an idealized version of the Stateflow/sup TM/ semantics. This paper describes how the principal Stateflow/sup TM/ execution rules are translated into SMV modules. Examples are used to illustrate the transformation procedures and their application to verify properties of executable specifications for control features in automotive powertrain control applications.
Instance Selection Techniques for Memory-Based Collaborative Filtering (2002)

Collaborative filtering (CF) has become an important data mining technique to make personalized recommendations for books, web pages or movies, etc. One popular algorithm is the memory-based collaborative filtering, which predicts a user's preference based on his or her similarity to other users (instances) in the database. However, the tremendous growth of users and the large number of products, memory-based CF algorithms results in the problem of deciding the right instances to use during prediction, in order to reduce executive cost and excessive storage, and possibly to improve the generalization accuracy by avoiding noise and overfitting. In this paper, we focus our work on a typical user preference database that contains many missing values, and propose four novel instance reduction techniques called TURF1-TURF4 as a preprocessing step to improve the efficiency and accuracy of the memory-based CF algorithm. The key idea is to generate prediction from a carefully selected set of relevant instances. We evaluate the techniques on the well-known EachMovie data set. Our experiments showed that the proposed algorithms not just dramatically speed up the prediction, but also improved the accuracy.
Digital RDS demodulation in FM subcarrier systems (2005)

We propose a digital receiver for FM subcarrier systems including the radio broadcast data systems (RBDS) and the radio data systems (RDS). The RBDS receiver is demodulating the 57 kHz modulated digital signal to the baseband RBDS digital signal. It contains carrier Costas loop synchronization techniques, a symbol timing recovery and decoding techniques, and an RBDS decoder. The RBDS decoder is used to recover the digital signal to the information data and sent to the output of the RBDS system. The main functions in the RBDS decoder are block error detection/correction, block synchronization, and block type detection.
Human Immunodeficiency Virus Type 1 Causes Productive Infection of Macrophages in Primary Placental Cell Cultures (1994)

To characterize the role of the placenta in vertical transmission of human immunodeficiency virus type 1 (HIV-1), the susceptibility of primary human placental cultures and of transformed trophoblast cell lines to infection by several HIV-1 isolates was examined. Placental cultures supported the replication of all strains tested, including lymphocyte-, macrophage-, and amphotropic isolates. All viruses replicated to modest levels, with production of both viral antigen and infectious virus in the culture supernatants. Placental cells demonstrated a pattern of permissiveness for HIV-1 isolates distinct from that seen with lymphocytes, blood-derived macrophages, or T cell lines. Immunofluorescent staining showed that 5%-10% of the cultured placental cells expressed viral antigens, and double labeling revealed that the HIV-positive cells were macrophages not trophoblasts. None of the trophoblast cell line (JEG-3, Jar, BeWo, HP-W1) could be infected by HIV. These results support the hypothesis that infection of the placenta could play a role in maternofetal transmission of HIV-1 and suggest that the placental macrophage is likely to be the primary cell type responsible.
Power aware job scheduling with QoS guarantees based on feedback control (2010)

With the scale of computing system increases, power consumption has become the major challenge to system performance, reliability and IT management costs. Specifically, system performance and reliability, described by various Quality of Service(QoS) metrics, cannot be guaranteed if the objective is to minimize the total power consumption solely, despite of the violations of QoS. Various methods have been developed to control power consumption to avoid system failures and thermal emergencies through coarse-grained designs. However, the existing methods can be improved and more power can be saved if fine-grained job level adaptation is integrated into them. In this paper a feedback control based power aware job scheduling algorithm is proposed to minimize power consumption in computing system and to provide QoS guarantees. In the proposed algorithm, jobs are scheduled according to the realtime and historical power consumption as well as the QoS requirements. Simulations and experiments on real multi core computing system show that the power potential of the system can be deeply explored while still providing QoS guarantees and the performance degradation is acceptable. The experiment results also show that fine-grained job-level power aware scheduling can achieve better power/performance balancing between multiple processors or cores than coarse-grained methods.
Characterization of a Proposed Oxytocin Receptor in the Uterus of the Rat and Sow (1974)

3H]Oxytocin, incubated with rat uterine segments, was bound specifically, and about equally, to the three particulate fractions obtained by homogenization and centrifugation at 1,000, 20,000, and 105,000 X g.There apparently was no binding in the cytosol fraction.["H]Oxytocin, added directly to rat uterine particles sedimentmg between 1,000 and 20,000 x g, was bound with an apparent & of 1.8 X 10qv M; 0.18 pmole of oxytocin was bound per mg of protein.For the 20,000 x g particulate fraction of the uterus from a pregnant sow, the apparent Kd for oxytocin binding was 1.5 X 10mv M; 0.15 pmole of oxytocin was bound per mg of protein.The affinity of oxytocin for the 105,000 x g particles from sow uterus was about 3 times greater than for the 20,000 x g particles.The binding constants are of the same order as the concentration of oxytocin which elicits a half-maximal uterine response.The binding of [aH]oxytocin by sow uterine 20,000 x g particles was maximal by 40 min at 20'.Less binding occurred at 37 and 0" than at 20".The competition for [sH]oxytocin binding sites by synthetic analogs of oxytocin was specific: oxytocin = [4-threonineloxytocin = [8-valineloxytocin > [&lysine]vasopressin >> [4-prolineloxytocin.Binding was optimal in the range of pH 7.4 to 7.8, was enhanced by Mn2+ and Co2+ > Mg2f > Zn2f, and was absent with 1 mu ethylenediaminetetraacetate.The concentrations of Mn2f giving maximal and half-maximal augmentation of binding were 5 and 0.4 mM, respectively.Ca2+, 5 mm, did not affect binding.A portion of the [aH]oxytocin appeared to be metabolized during the incubation with sow uterine particles.The uterine oxytocin receptor site is protein, at least in part, because binding was reduced by trypsin, -SH reagents such as N-ethylmaleimide, p-chloromercuribenzoate, and by thioglycollate.Phospholipases A and C inhibited binding; however, their inhibitory activities appeared to be separate from their catalytic activities.Neuraminidase, phospholipase D, butanedione, and sodium fluoride did not affect [aH]oxytocin binding.Binding was depressed by guanosine triphosphate, cytosine triphosphate, and uridine triphosphate.
Classifying flows and buffer state for youtube's HTTP adaptive streaming service in mobile networks (2018)

Accurate cross-layer information is very useful to optimize mobile networks for specific applications. However, providing application-layer information to lower protocol layers has become very difficult due to the wide adoption of end-to-end encryption and due to the absence of cross-layer signaling standards. As an alternative, this paper presents a traffic profiling solution to passively estimate parameters of HTTP Adaptive Streaming (HAS) applications at the lower layers. By observing IP packet arrivals, our machine learning system identifies video flows and detects the state of an HAS client's play-back buffer in real time. Our experiments with YouTube's mobile client show that Random Forests achieve very high accuracy even with a strong variation of link quality. Since this high performance is achieved at IP level with a small, generic feature set, our approach requires no Deep Packet Inspection (DPI), comes at low complexity, and does not interfere with end-to-end encryption. Traffic profiling is, thus, a powerful new tool for monitoring and managing even encrypted HAS traffic in mobile networks.
Biochemical Characterization and Cellular Effects of CADASIL Mutants of NOTCH3 (2012)

Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is the best understood cause of dominantly inherited stroke and results from NOTCH3 mutations that lead to NOTCH3 protein accumulation and selective arterial smooth muscle degeneration. Previous studies show that NOTCH3 protein forms multimers. Here, we investigate protein interactions between NOTCH3 and other vascular Notch isoforms and characterize the effects of elevated NOTCH3 on smooth muscle gene regulation. We demonstrate that NOTCH3 forms heterodimers with NOTCH1, NOTCH3, and NOTCH4. R90C and C49Y mutant NOTCH3 form complexes which are more resistant to detergents than wild type NOTCH3 complexes. Using quantitative NOTCH3-luciferase clearance assays, we found significant inhibition of mutant NOTCH3 clearance. In coculture assays of NOTCH function, overexpressed wild type and mutant NOTCH3 significantly repressed NOTCH-regulated smooth muscle transcripts and potently impaired the activity of three independent smooth muscle promoters. Wildtype and R90C recombinant NOTCH3 proteins applied to cell cultures also blocked canonical Notch fuction. We conclude that CADASIL mutants of NOTCH3 complex with NOTCH1, 3, and 4, slow NOTCH3 clearance, and that overexpressed wild type and mutant NOTCH3 protein interfere with key NOTCH-mediated functions in smooth muscle cells.
Tau filaments from human brain and from <i>in vitro</i> assembly of recombinant protein show cross-β structure (2003)

Abnormal filaments consisting of hyperphosphorylated microtubule-associated protein tau form in the brains of patients with Alzheimer's disease, Down's syndrome, and various dementing tauopathies. In Alzheimer's disease and Down's syndrome, the filaments have two characteristic morphologies referred to as paired helical and straight filaments, whereas in tauopathies, there is a wider range of morphologies. There has been controversy in the literature concerning the internal molecular fine structure of these filaments, with arguments for and against the cross-beta structure demonstrated in many other amyloid fibers. The difficulty is to produce from brain pure preparations of filaments for analysis. One approach to avoid the need for a pure preparation is to use selected area electron diffraction from small groups of filaments of defined morphology. Alternatively, it is possible to assemble filaments in vitro from expressed tau protein to produce a homogeneous specimen suitable for analysis by electron diffraction, x-ray diffraction, and Fourier transform infrared spectroscopy. Using both these approaches, we show here that native filaments from brain and filaments assembled in vitro from expressed tau protein have a clear cross-beta structure.
SiO<inf>2</inf>/SiN infrared absorbing films for uncooled pyroelectric sensor and its fabrication and evaluation (2013)

In this paper, we report a development of fabrication processes and characteristics of uncooled pyroelectric infrared sensor using epitaxial Pb(Zr,Ti)O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> thin film on γ-Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> /Si substrate. Newly designed SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> /SiN double layer absorbing film is calculated and evaluated to fabricate on the sensor for infrared absorption efficiency improvement. The absorption of 70% is achieved with the double layer thickness of 1.4 μm in wavelength between 8 to 12 μm. Infrared responsivity of 176 V/W is obtained at 10 Hz.
Correlation of the Base Strengths of Amines<sup>1</sup> (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCorrelation of the Base Strengths of Amines1H. K. Hall Jr.Cite this: J. Am. Chem. Soc. 1957, 79, 20, 5441–5444Publication Date (Print):October 1, 1957Publication History Published online1 May 2002Published inissue 1 October 1957https://pubs.acs.org/doi/10.1021/ja01577a030https://doi.org/10.1021/ja01577a030research-articleACS PublicationsRequest reuse permissionsArticle Views7175Altmetric-Citations679LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Isolation of a Bacteriophage Specific for a New Capsular Type of Klebsiella pneumoniae and Characterization of Its Polysaccharide Depolymerase (2013)

Here we report that a group of clinical strains possess a novel Klebsiella capsular type. We identified a KN2-specific phage and its polysaccharide depolymerase, which could be used for efficient capsular typing. The lytic phage and depolymerase also have potential as alternative therapeutic agents to antibiotics for treating K. pneumoniae infections, especially against antibiotic-resistant strains.
Optical absorption edge characteristics of cubic boron nitride thin films (1999)

Boron nitride films with different cubic phase contents were deposited in a radio frequency bias sputtering system by a two-stage deposition process. The Fourier transform infrared spectra and UV-visible transmittance and reflection spectra were measured. The optical absorption edge of BN films consists of a low energy region in which α increases exponentially as incident photon energy hν, and a high energy region, in which α varies as (hν)m, which is the characteristics of amorphous materials. These two regions are fitted by the Urbach tail model and the band-to-band transition model with an effective medium approach, and the Urbach energy and the optical band gap are determined from these fits. With an increase in the cubic boron nitride (c-BN) content, the absorption edge shifts to the higher energy and the optical band gap increases. For a BN film with 88% cubic phase, the optical band gap exceeds 6.0 eV, which is comparable to that of c-BN single crystal.
Predictive markers in colorectal cancer: where are we now? (2012)

Heinz-Josef Lenz speaks to Ruth Williamson, Commissioning Editor: Heinz-Josef Lenz is Professor of Medicine and Professor of Preventive Medicine, and holds the Kathryn Balakrishnan Chair for Cancer Research. He is associate director of clinical research and co-chair of the Gastrointestinal Oncology Program at the University of Southern California (USC) Center for Molecular Pathways and Drug Discovery at the University of Southern California Keck School of Medicine and USC/Norris Comprehensive Cancer Center in Los Angeles (CA, USA). Dr Lenz received his medical degree from Johannes-Gutenberg Universität in Mainz, Germany, and went on to complete a residency in hematology and oncology at the Eberhard-Karls Universität Tübingen, Germany, before completing clerkships in oncology and hematology at George Washington University (WA, USA) and the Beth Israel Hospital of Harvard Medical School (MA, USA), respectively. He completed a postdoctoral fellowship in the Department of Biochemistry and Molecular Biology at the USC Keck School of Medicine. Dr Lenz’s research focus is in the identification of biomarkers in gastrointestinal cancers, early drug development and novel clinical trials. He is co-chair of Southwest Oncology Group Gastrointestinal Committee, co-chair of Southwest Oncology Group Gastrointestinal Translational Medicine and a member of the NCI Gastrointestinal Steering Committee, NCI Gastroesophageal Task Force and NCI Correlative Science Committee. He is the USC principal investigator on the U01, N01 and U10 NIH grants and NIH contract with City of Hope and the University of California Davis.
<i>Epidermal Growth Factor Receptor</i> Gene Amplification Is Acquired in Association with Tumor Progression of <i>EGFR</i>-Mutated Lung Cancer (2008)

Both mutation and amplification of epidermal growth factor receptor (EGFR) in lung cancers have been reported in association with clinical responses to tyrosine kinase inhibitors. We have reported evidence implicating mutation specifically in the "terminal respiratory unit" type of adenocarcinoma, which is characterized by expression of thyroid transcription factor 1, a lineage marker of peripheral airway cells. However, little is known about the role of gene amplification in the molecular progression of lung adenocarcinoma. In this study, we examined the topographical distribution of amplification in three microdissected portions each of 48 individual lung cancers with confirmed mutations. Relative copy number of the gene was analyzed using Taq Man-based gene dosage analysis and fluorescent in situ hybridization technique. Gene amplification was found in 11 lung cancers. Strikingly, nine of the cancers showed heterogeneous distribution, and amplification was associated with higher histologic grade or invasive growth. Because it was likely that the high-grade lesions were the origin for metastases, metastatic lymph nodes corresponding to five tumors with heterogeneous distribution were analyzed. Unexpectedly, amplification status of the metastatic sites was not always associated with gene amplification of the primary tumors, suggesting that selection of the metastatic clone may be defined by other factors. We also examined 17 precursor lesions and 21 in situ lung adenocarcinomas, and found that only one in situ carcinoma harbored gene amplification. Taken together, our results show that mutation occurs early in the development of lung adenocarcinoma, and that amplification may be acquired in association with tumor progression.
Jeremiah. Volume I. (<i>The Daily Study Bible</i>.) By Robert Davidson. Edinburgh, The St. Andrew Press, 1983. Pp. 165. £2·95. - Psalms. Volume II. (<i>The Daily Study Bible</i>.) By G. A. F. Knight. Edinburgh, The St. Andrew Press, 1983. Pp. 368. £2·95. (1985)

Jeremiah. Volume I. (The Daily Study Bible.) By Robert Davidson. Edinburgh, The St. Andrew Press, 1983. Pp. 165. £2·95. - Psalms. Volume II. (The Daily Study Bible.) By G. A. F. Knight. Edinburgh, The St. Andrew Press, 1983. Pp. 368. £2·95. - Volume 38 Issue 1
Clinical heterogeneity and novel mutations in the glycerol kinase gene in three families with isolated glycerol kinase deficiency. (1998)

Isolated glycerol kinase deficiency (GKD) is an X linked recessive disorder. The clinical and biochemical picture may vary from a childhood metabolic crisis to asymptomatic adult "pseudohypertriglyceridaemia", the result of hyperglycerolaemia. We performed glycerol kinase (GK) gene analysis to study the molecular heterogeneity and genotype-phenotype correlation in eight males from three families with isolated GKD. All patients had hyperglycerolaemia and glyceroluria. Four patients from two families were essentially free of symptoms. Three patients had gastrointestinal symptoms with ketoacidosis or hypoglycaemia or both. One patient had recurrent convulsions as the only acute sign, without evidence that it was correlated with a catabolic state. Fasting tests in two symptomatic patients of family 1 showed hyperketotic states, together with a tendency to hypoglycaemia. The diagnosis was confirmed by a defective 14C-glycerol incorporation into trichloroacetic acid precipitable macromolecules in intact skin fibroblasts. Mutation screening of the GK gene was performed by amplification and direct sequencing of exons using PCR. Three novel mutations were identified: (1) a deletion starting downstream of exon 9, extending to the 3' end of the gene; (2) a nonsense mutation R413X caused by a C1351T transition; and (3) a missense mutation W503R caused by a T1651C transition. In addition, we found differences from the reported sequence: (1) exon 9 actually consists of two exons, which consequently will change the number of GK gene exons from 19 to 20 exons, and (2) nucleotide differences in exon 19. So far, no genotype-phenotype correlation can be established in these GKD families.
PHOTOCLEAVAGE OF COUMARIN DIMERS (1972)

Abstract trans-head-to-head Coumarin dimer cleaves into coumarin in chloroform by irradiation with the light of wavelength 277 nm in high yield. When a ring opened dimer, trans-3,cis-4-bis(2-hydroxyphenyl)-l,trans-2-cyclobutanedicarboxylic acid in aqueous solution is irradiated, a mixture of trans-2,2′-dihydroxystilbene and fumaric acid is obtained in high yield.