gebhart/patrepeval huggingface dataset:
Molecular Profiling of Experimental Parkinson's Disease:  Direct Analysis of Peptides and Proteins on Brain Tissue Sections by MALDI Mass Spectrometry (2004)

Direct molecular profiling of biological samples using matrix-assisted laser desorption ionization mass spectrometry is a powerful tool for identifying phenotypic markers. In this report, protein profiling was used for the first time to generate peptide and protein profiles of brain tissue sections obtained from experimental Parkinson's disease (unilaterally 6-hydroxydopamine treated rats). The mass spectrometer was used to map the peptide and protein expression directly on 12 microm tissue sections in mass-to-charge (m/z) values, providing the capability of mapping specific molecules of the original sample, that is, localization, intensity and m/z ratio. Several protein expression profile differences were found in the dopamine depleted side of the brain when compared to the corresponding intact side, for example, calmodulin, cytochrome c, and cytochrome c oxidase. An increased ratio of post-translational modifications such as acetylations were found in the striatum of proteins in the dopamine depleted side of the brain. These modifications were decreased after subchronic administration of L-Dopa. The present study shows that unique protein profiles can be obtained in specific brain regions (and subregions) directly on brain tissue sections and allows for the study of complex biochemical processes such as those occurring in experimental Parkinson's disease.
G<scp>ra</scp>S<scp>p</scp>: Combining Spatially‐aware Mobile Devices and a Display Wall for Graph Visualization and Interaction (2017)

Abstract Going beyond established desktop interfaces, researchers have begun re‐thinking visualization approaches to make use of alternative display environments and more natural interaction modalities. In this paper, we investigate how spatially‐aware mobile displays and a large display wall can be coupled to support graph visualization and interaction. For that purpose, we distribute typical visualization views of classic node‐link and matrix representations between displays. The focus of our work lies in novel interaction techniques that enable users to work with personal mobile devices in combination with the wall. We devised and implemented a comprehensive interaction repertoire that supports basic and advanced graph exploration and manipulation tasks, including selection, details‐on‐demand, focus transitions, interactive lenses, and data editing. A qualitative study has been conducted to identify strengths and weaknesses of our techniques. Feedback showed that combining mobile devices and a wall‐sized display is useful for diverse graph‐related tasks. We also gained valuable insights regarding the distribution of visualization views and interactive tools among the combined displays.
Comparative genome analysis of lignin biosynthesis gene families across the plant kingdom (2009)

The research revealed that, among the species analyzed, the complete lignin biosynthesis pathway first appeared in moss; the pathway is absent in green algae. The expansion of lignin biosynthesis gene families correlates with substrate diversity. In addition, we found that the expansion of the gene families mostly occurred after the divergence of monocots and dicots, with the exception of the C4H gene family. Gene expression analysis revealed different fates of gene duplications, largely confirming plants are tolerant to gene dosage effects. The rapid expansion of lignin biosynthesis genes indicated that the translation of transgenic lignin modification strategies from model species to bioenergy feedstock might only be successful between the closely relevant species within the same family.
Prostaglandin E-mediated mitogenic stimulation of mouse epidermis in vivo by divalent cation ionophore A 23187 and by tumor promoter 12-O-tetradecanoylphorbol-13-acetate. (1981)

When applied to mouse skin in vivo, both the strong tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) (2 nmol) and the divalent cation ionophore A 23187 (200 nmol) caused the same responses, i.e., skin inflammation and prostaglandin E2-mediated epidermal hyperplasia. In both cases, these events were accompanied by certain biochemical reactions in the epidermis such as an increase in the biosynthesis of and sensitivity to prostaglandin E2, increase in ornithine decarboxylase and phosphodiesterase activities, and refractoriness of cyclic adenosine 3':5'-monophosphate production to beta-adrenergic stimulation. In contrast to A 23187, TPA did not induce degranulation of mast cells; whereas, in contrast with TPA, A 23187 did not show tumor-promoting activity. These results indicate that the observed biological effects of TPA are no indication of tumor-promoting ability and that, on the other hand, the mitogenic effects of A 23187 are possibly not due to its properties as a calcium ionophore.
Phase III Trial of Radiosensitizer PR-350 Combined With Intraoperative Radiotherapy for the Treatment of Locally Advanced Pancreatic Cancer (2004)

A randomized, controlled trial was conducted to clarify the effect of novel radiosensitizer, PR-350, accompanied by intraoperative radiology (IOR) on locally advanced pancreatic cancer. Between July 1999 and March 2002, 48 patients were enrolled in this clinical trial and received either PR-350 or placebo. Any differences between the PR-350 group (n = 22) and control group (n = 25) were not statically significant. All patients were evaluated, and none of them showed toxicity, with the exception of 1 patient from the control group, and the PR-350 compound was considered to be safe. The efficacy of IOR with PR-350 was evaluated using CT examination. The committee responsible for evaluating efficacy reported that 47.4% of the PR-350 group showed the effective response, compared with 21.7% of the control group (P = 0.1067, Fisher analysis). At 6 months following treatment, the tumor mass reduction rate in the PR-350 group was significantly improved (P = 0.0274). By the time of the last follow-up in July 2003, 17 PR-350 patients and 24 control patients group had died of the disease. The median survival period of the PR-350 group was thus 318.5 days and that of the control group is 303.0 days. One-year survival rates of the PR-350 group and control group were 36.4% and 32.0%, respectively. Although the PR-350 group did not demonstrate significantly better survival than the control group, 4 of 22 PR-350 patients were still living more than 2 years after the end of the trial, compared with only 1 of 25 patients from the control group. The mechanism of this increased therapeutic response to radiotherapy using PR-350 must be clarified to establish more effective strategy for pancreatic cancer treatment.
Enhanced transdermal derivery of zidovudine in rats and human skin. (1990)

Zidovudine (azidothymidine, AZT), a potent antiviral agent acting on acquired immunodeficiency syndrome virus, was examined with regard to permeation through rat and human skin. A steady state plasma concentration of AZT after transdermal application in rats estimated from both pharmacokinetics data after i.v. administration and penetration rate through excised rat skin from 10% oleic acid (OA) aqueous solution shows penetration about 85 times higher compared to that from 10% OA would be needed for therapeutic efficacy. A mixed-solvent system consisted of 5% Sefsol-318 (S-318), 10% OA, 10% N-methyl-2-pyrrolidone (MP), 20% propylene glycol (PG) and water showed promising characteristics as a vehicle in terms of permeability of AZT through excised rat skin. The maximum flux of 0.41 mumol/cm2/h was observed in excised human skin after application of a gel formulation including S-318, OA, MP and PG. The result suggests a possible use of the gel formulation to gain an effective plasma concentration in humans.
The effect of airway interference on the growth and development of the face, jaws, and dentition (1982)

Two criteria of great importance for the well­being of most humans are good health and appearance. This paper is an attempt to examine the effect of allergies on both those criteria as far as the growth and development of the face and dentition are concerned. A review of the literature shows that allergies are responsible for nasorespiratory blockage either through congestion of the nasal mucous membranes or through the hypertrophy of tonsils and adenoids. Several articles report the incidence and age of onset of these phenomena. The development of immunocompetent lymphoid tissue in the nasopharynx is correlated with the growth of the nasopharyngeal area. An imbalance in the equilibrium of differential growth of these organs can result in mouth breathing. This paper will examine the consequences of mouth breathing as documented in studies performed on experimental animals, and as seen in clinical examinations of children. The review of the literature will also show that upon treatment of the allergic condition or surgical removal of the excessive tonsil and adenoid tissues it was found that the restoration of nasal breathing reduced the malformations present and resulted in a tendency towards normalization of health and appearance.
Metal-organic and zeolite imidazolate frameworks (MOFs and ZIFs) for highly selective separations (2012)

Metal-organic and zeolite imidazolate frameworks (MOFs and ZIFs) have been investigated for the realization as separation media with high selectivity. These structures are held together with strong bonds, making them architecturally, chemically, and thermally stable. Therefore, employing well designed building units, it is possible to discover promising materials for gas and vapor separation. This grant was focused on the study of MOFs and ZIFs with these specific objectives: (i) to develop a strategy for producing MOFs and ZIFs that combine high surface areas with active sites for their use in gas adsorption and separation of small organic compounds, (ii) to introduce active sites in the framework by a post-synthetic modification and metalation of MOFs and ZIFs, and (iii) to design and synthesize MOFs with extremely high surface areas and large pore volumes to accommodate large amounts of guest molecules. By the systematic study, this effort demonstrated how to introduce active functional groups in the frameworks, and this is also the origin of a new strategy, which is termed isoreticular functionalization and metalation. However, a large pore volume is still a prerequisite feature. One of the solutions to overcome this challenge is an isoreticular expansion of a MOF's structure. With triangular organic linker and square building units, we demonstrated that MOF-399 has a unit cell volume 17 times larger than that of the first reported material isoreticular to it, and it has the highest porosity (94%) and lowest density (0.126 g cm-3) of any MOF reported to date. MOFs are not just low density materials; the guest-free form of MOF-210 demonstrates an ultrahigh porosity, whose BET surface area was estimated to be 6240 m2 g-1 by N2 adsorption measurements.
Aggravation of polycystic kidney disease in Han (1997)

The administration of ammonium chloride or of sodium or potassium bicarbonate has marked effects on the development of polycystic kidney disease (PKD) in Han:SPRD rats. Because of the possibility that these effects are mediated by changes in redox metabolism, the aim of this study was to determine whether depletion of glutathione, the most abundant and important cellular thiol and scavenger of reactive oxygen species, would affect the development of PKD in this animal model. +/+ and cy/+ Han:SPRD rats were treated with: (1) L-buthionine(S,R)-sulfoximine (BSO), a specific inhibitor of gamma-glutamylcysteine synthetase, the rate-limiting enzyme for the synthesis of glutathione; (2) glutathione monoethyl ester (GME), a compound that is known to increase the intracellular levels of glutathione; or (3) BSO and GME. Treatment with these drugs was started at 3 wk of age, and the animals were killed at 6 or 8 wk of age. Renal levels of oxidized glutathione were significantly higher in cy/+ than in +/+ rats, whereas no significant differences in reduced glutathione were detected. The administration of BSO caused a marked reduction in the levels of glutathione. The administration of GME caused a significant increase in the levels of glutathione at 2 h, but not at 12 h, after the administration. The increase in the renal levels of glutathione 2 h after the administration of GME was less in the rats treated with BSO than in the rats not receiving this drug, indicating that in part the increase in glutathione level was due to de novo synthesis. BSO-induced glutathione depletion was accompanied by a marked aggravation of the renal cystic disease, as reflected by kidney weights, histological scores, and plasma urea concentrations. However, the administration of GME did not lessen the cystic disease and did not reverse the effects of BSO. The transient effect of GME administration and the simultaneous increases in the levels of cysteine and oxidized glutathione, in addition to reduced glutathione, may explain the lack of protection by GME. These data support the notion that changes in redox metabolism may affect the development of PKD.
ChemInform Abstract: ′CRYPTOMYCINS′ ‐ CROWN ETHER ANALOGS OF THE ACTINOMYCINS (1979)

Abstract Die Azakronenether (VI) und (VIII), Analoge von Actinomycin D (I) mit ähnlichen Hohlraum‐Dimensionen werden synthetisiert: Kondensation der (bekannten) Komponenten (II) und (III) gibt die Verbindung (IV), aus der dann über die Stufen (V) bzw. (VII) die Titelverbindungen (VI) (′Cryptomycin‐B′) bzw. (VIII) (′Cryptomycin‐A′) erhältlich sind.
Rank/Rankl/opg: literature review. (2012)

The discovery of the receptor activator of nuclear factor-kB (RANK)/RANK Ligand (RANKL)/osteoprotegerin (OPG) pathway contributed to the understanding of how bone formation and resorption were processed and regulated. RANKL and OPG are members of the tumor necrosis factor (TNF) and TNF receptor (TNFr) superfamilies, respectively, and binding to receptor activator of NF-kB (RANK) not only regulate osteoclast formation, activation and survival in normal bone modeling and remode-ling, but also in several other pathologic conditions characterized by increased bone turnover. There is accumulating evidence of the potential role of OPG and RANKL in other tissues. Looking beyond the RANK/RANKL/OPG axis, Wingless (Wnt) pathway emerged as the osteoblast differentiation way, and also as a bone mass regulator. Researchers have been discovering new molecules and cytokines interactions. Altogether, data suggest that RANK/RANKL/OPG system could be targeted as a new treatment strategy in bone conditions. FREEDOM is the more recently published clinical trial about a RANKL-specific recombinant fully human monoclonal antibody (denosumab). OPG is also a potential innovative therapeutic option to be investigated.
Enhancing automated teller machine with multi-lingual and multi-denominational software functionalities (2013)

In this study, we conceptualise a multi-lingual and multi-denominational software for automated teller machine (ATM). The purpose is to model an ATM that communicates in different pre-defined languages and dispenses cash in different denominations based on the customers' choices, thereby enhancing the ease of use by customers and reducing frustrations by rural dwellers. The architecture of the system was developed using the unified modelling language, whereas the prototype was developed using Java programming language for the front-end, and Oracle database management system as the back-end. The prototype was tested using some hypothetical data and was found to successfully mimic ATM transactions in the local Nigerian languages. The results of the study also point to an improvement in the user interface with icons and music being more friendly and welcoming to the local populace.
UVB-mediated activation of p38 mitogen-activated protein kinase enhances resistance of normal human keratinocytes to apoptosis by stabilizing cytoplasmic p53 (2002)

Human keratinocytes respond to UV rays by developing a fast adaptive response that contributes to maintaining their functions and survival. We investigated the role of the mitogen-activated protein kinase pathways in transducing the UV signals in normal human keratinocytes. We found that UVA, UVB or UVC induced a marked and persistent activation of p38, whereas c-Jun N-terminal kinase or extracellular signal-regulated kinase were less or not activated respectively. Inhibition of p38 activity by expression of a dominant-negative mutant of p38 or with SB203580 impaired cell viability and led to an increase in UVB-induced apoptosis. This sensitization to apoptosis was independent of caspase activities. Inhibition of p38 did not sensitize transformed HaCaT keratinocytes to UVB-induced apoptosis. In normal keratinocytes, expression of a dominant-negative mutant of p53 increased UVB-induced cell death, pointing to a role for p53. In these cells, UVB triggered a p38-dependent phosphorylation of p53 on Ser-15. This phosphorylation was associated with an SB203580-sensitive accumulation of p53, even in the presence of a serine phosphatase inhibitor. Accumulated p53 was localized mainly in the cytoplasm, independently of CRM1 nuclear export. In HaCaT cells, p53 was localized exclusively in the nucleus and its distribution and level were not affected by UVB or p38 inhibition. However, UVB induced an SB203580-insensitive phosphorylation on Ser-15 of mutated p53. Overall, our results suggest that, in normal human keratinocytes, protection against UVB depends on p38-mediated phosphorylation and stabilization of p53 and is tightly associated with the cytoplasmic sequestration of wild-type p53. We conclude that the p38/p53 pathway plays a key role in the adaptive response of normal human keratinocytes against UV stress.
A 1V 663&#x00B5;W 15-bit audio &#x0394;&#x03A3; modulator in 0.18&#x00B5;m CMOS (2010)

A 15-bit 3 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">rd</sup> order ΔΣ modulator is presented. The feed forward topology with 18-level quantizer is adopted. The signal swing of the 1 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">st</sup> integrator is effectively suppressed. A current-mirror OTA with 42dB DC gain is used in the 1 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">st</sup> integrator. Chop stabilization is employed to remove the flicker noise. The prototype is fabricated in 0.18μm CMOS. The active die area is 0.85×0.85mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . The power consumption is 663μW under all 1V power supply.
Catalytic two-stage hydrocracking of Arabian vacuum residue at a high conversion level without sludge formation (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalytic two-stage hydrocracking of Arabian vacuum residue at a high conversion level without sludge formationIsao Mochida, Xing Zhe Zhao, and Kinya SakanishiCite this: Ind. Eng. Chem. Res. 1990, 29, 3, 334–337Publication Date (Print):March 1, 1990Publication History Published online1 May 2002Published inissue 1 March 1990https://pubs.acs.org/doi/10.1021/ie00099a005https://doi.org/10.1021/ie00099a005research-articleACS PublicationsRequest reuse permissionsArticle Views200Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Impact of mutant p53 functional properties on<i>TP53</i>mutation patterns and tumor phenotype: lessons from recent developments in the IARC TP53 database (2007)

The tumor suppressor gene TP53 is frequently mutated in human cancers. More than 75% of all mutations are missense substitutions that have been extensively analyzed in various yeast and human cell assays. The International Agency for Research on Cancer (IARC) TP53 database (www-p53.iarc.fr) compiles all genetic variations that have been reported in TP53. Here, we present recent database developments that include new annotations on the functional properties of mutant proteins, and we perform a systematic analysis of the database to determine the functional properties that contribute to the occurrence of mutational "hotspots" in different cancer types and to the phenotype of tumors. This analysis showed that loss of transactivation capacity is a key factor for the selection of missense mutations, and that difference in mutation frequencies is closely related to nucleotide substitution rates along TP53 coding sequence. An interesting new finding is that in patients with an inherited missense mutation, the age at onset of tumors was related to the functional severity of the mutation, mutations with total loss of transactivation activity being associated with earlier cancer onset compared to mutations that retain partial transactivation capacity. Furthermore, 80% of the most common mutants show a capacity to exert dominant-negative effect (DNE) over wild-type p53, compared to only 45% of the less frequent mutants studied, suggesting that DNE may play a role in shaping mutation patterns. These results provide new insights into the factors that shape mutation patterns and influence mutation phenotype, which may have clinical interest.
Distributed MEMS true-time delay phase shifters and wide-band switches (1998)

Wide-band switches and true-time delay (TTD) phase shifters have been developed using distributed microelectromechanical system (MEMS) transmission lines for applications in phased-array and communication systems. The design consists of a coplanar waveguide (CPW) transmission line (W=G=100 /spl mu/m) fabricated on a 500 /spl mu/m quartz substrate with fixed-fixed beam MEMS bridge capacitors placed periodically over the transmission line, thus creating a slow-wave structure. A single analog control voltage applied to the center conductor of the CPW line can vary the phase velocity of the loaded line by pulling down on the MEMS bridges to increase the distributed capacitive loading. The resulting change in the phase velocity yields a TTD phase shift. Alternatively, the control voltage can be increased beyond the pull-down voltage of the MEMS bridges such that the capacitive loading greatly increases and shorts the line to ground. The measured results demonstrate 0-60 GHz TTD phase shifters with 2 dB loss/118/spl deg/ phase shift at 60 GHz (/spl sim/4.5-ps TTD) and 1.8 dB loss/84/spl deg/ phase shift at 40 GHz. Also, switches have been demonstrated with an isolation of better than 40 dB from 21 to 40 and 40 to 60 GHz. In addition, a transmission-line model has been developed, which results in very close agreement with the measured data for both the phase shifters and switches. The pull-down voltage is 10-23 V, depending on the residual stress in the MEMS bridge. To our knowledge, this paper presents the first wide-band TTD MEMS phase shifters and wide-band switches to date.