gebhart/patrepeval huggingface dataset:
AIB1 enhances estrogen-dependent induction of cyclin D1 expression. (2001)

AIB1 was isolated as a gene amplified in breast cancer and encodes a protein that acts as a steroid receptor coactivator. The role of steroid receptor coactivators such as AIB1 in breast cancer development is not clear. It is possible that AIB1 cooperates with estrogen receptor alpha in regulating estrogen-dependent cell proliferation. Ectopic expression of the estrogen receptor alpha in different cell lines does not confer estrogen-induced proliferation. This inability of the estrogen receptor to drive proliferation has been recently correlated with a lack of estrogen-dependent cyclin D1 expression in cells engineered to express the estrogen receptor. In this study, we evaluated whether high levels of AIB1 enable the estrogen receptor to direct the transcription of cyclin D1. We show here that AIB1 and other steroid receptor coactivators can enhance the functional interaction of the estrogen receptor with the cyclin D1 promoter. Increases of AIB1 levels in breast cancer cells by amplification and/or overexpression may represent one way to confer estrogen-dependent mitogenic stimulation to breast cancer cells.
A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography (2005)

Membrane separation and chromatographic technologies are regarded as an attractive alternative to conventional academic small-scale ultracentrifugation procedures used for retrovirus purification. However, despite the increasing demands for purified retroviral vector preparations, new chromatography adsorbents with high specificity for the virus have not been reported. Heparin affinity chromatography is presented here as a novel convenient tool for retrovirus purification. The ability of bioactive retroviral particles to specifically bind to heparin ligands immobilized on a chromatographic gel is shown. A purification factor of 63 with a recovery of 61% of functional retroparticles was achieved using this single step. Tentacle heparin affinity supports captured retroviral particles more efficiently than conventional heparin affinity chromatography supports with which a lower recovery was obtained (18%). Intact, infective retroviral particles were recovered by elution with low salt concentrations (350 mM NaCl). Mild conditions for retrovirus elution from chromatographic columns are required to preserve virus infectivity. VSV-G pseudotyped retroviruses have shown to be very sensitive to high ionic strength, losing 50% of their activity and showing membrane damage after a short exposure to 1M NaCl. We also report a complete scaleable downstream processing scheme for the purification of MoMLV-derived vectors that involves sequential microfiltration and ultra/diafiltration steps for virus clarification and concentration respectively, followed by fractionation by heparin affinity chromatography and final polishing by size-exclusion chromatography. Overall, by using this strategy, a 38% yield of infective particles can be achieved with a final purification factor of 2,000.
Cancer Screening Test Use — United States, 2015 (2017)

Healthy People 2020 (HP2020) includes objectives to increase screening for breast, cervical, and colorectal cancer (1) as recommended by the U.S. Preventive Services Task Force (USPSTF).* Progress toward meeting these objectives is monitored by measuring cancer screening test use against national targets using data from the National Health Interview Survey (NHIS) (1). Analysis of 2015 NHIS data indicated that screening test use remains substantially below HP2020 targets for selected cancer screening tests. Although colorectal cancer screening test use increased from 2000 to 2015, no improvements in test use were observed for breast and cervical cancer screening. Disparities exist in screening test use by race/ethnicity, socioeconomic status, and health care access indicators. Increased measures to implement evidence-based interventions and conduct targeted outreach are needed if the HP2020 targets for cancer screening are to be achieved and the disparities in screening test use are to be reduced.
Maternal inheritance of Cre activity in a <i>Sox2Cre</i> deleter strain (2003)

The Sox2 gene is expressed in several undifferentiated cell types. In an earlier study we described a Sox2Cre transgene that mediates epiblast-specific Cre-mediated modification of gene activity in the embryo. Here we report that this transgene is active in the female germline. Consequently, all offspring that arise from female mice heterozygous for the Sox2Cre transgene have demonstrable Cre activity irrespective of whether they inherit the transgene itself. Maternal inheritance of Cre activity allows the efficient modification of gene activity for functional analysis.
Body Shaping and Volume Restoration: The Role of Hyaluronic Acid (2009)

Driven by the rising popularity of minimally invasive techniques, the demand for cosmetic procedures is increasing. Cosmetic body-shaping procedures can be categorized into those that remove tissue and those that add volume. This review focuses on the latter of these categories, particularly on the use of resorbable hyaluronic acid gels specifically developed for minimally invasive volume enhancement. Pilot studies of hyaluronic acid involving its injection to contour various body deformities and its recent use in female breast augmentation are discussed. Injectable hyaluronic acid is effective and well tolerated. It represents an attractive treatment option for volume restoration or augmentation by providing predictable long-lasting results after minimally invasive administration. Alternative treatment options for volume enhancement also are summarized including fat transfer, silicone implants, and the use of injectable nonresorbable products such as silicone, polyalkylimide, and polyacrylamide gels. As patients continue to opt for nonsurgical procedures that offer predictable results, the development of minimally invasive products such as hyaluronic acid is increasingly important.
Short communication (1986)

The use of the vacuum extractor at cesarean section has been advocated in order to avoid extension of the uterine incision and to facilitate the delivery of a floating head. We have evaluated, prospectively and retrospectively, the neonatal outcome of vacuum extraction at cesarean section in comparison with regular cesarean section. The interval between the final uterine incision and complete delivery (U-D interval) was significantly prolonged (P less than 0.01) in 8 cases of vacuum extraction (Group A) when prospectively compared with 10 cases of regular cesarean section (Group B). The Apgar scores, the acid-base values at delivery and the clinical course of the two groups were not significantly different. Although analysis of our prospective data suggests a trend of an association between prolongation of the U-D interval and neonatal depression, our retrospective data of 34 neonates delivered by vacuum extraction at cesarean section and 44 neonates delivered by regular cesarean section, showed no significant untoward effect when the vacuum extractor was utilized. Since prolongation of the U-D interval may have an undesireable effect on the fetus, we advocate extra caution in the use of this method.
Small World (2008)

In this chapter we consider from a marketing perspective the political, cultural/social, and economic factors, both micro and macro, affecting the supply/demand nexus of broadband services for the Irish consumer. We chart the development of broadband and its current situation of rollout and uptake, examine the reasons for its continuing poor performance, and offer recommendations on how Ireland may close the gap and perhaps even move ahead. We collated data from a variety of resources, journals and press and trade publications. We attended a ministerial conference on the state of broadband to which many representatives of the telecommunications industry had been invited. We surveyed people with and without the facility, and interviewed key players in the field. We concluded that, although the market is beginning to grow strongly, it is from a low base, and as a result the country still lags behind many of its European counterparts. There is still a lack of competition which is having an adverse effect on both supply and demand of broadband for the Irish domestic consumer. In a small country, the incumbent still holds control of the most profitable areas of the technology, particularly wholesale and selling to other operators. Also, the Irish are still not convinced that broadband is the ‘killer app’ they need. This may change in the future as the technology delivers more of the content-rich multimedia fare that the Irish already enjoy in other formats.
Facile Method for the Combinatorial Synthesis of 2,2-Disubstituted Quinazolin-4(1<i>H</i>)-one Derivatives Catalyzed by Iodine in Ionic Liquids (2010)

A mild and facile method for the combinatorial synthesis of quinazolin-4-(1H)-one derivatives, containing simple 2,2-disubstituted quinazolin-4-(1H)-ones, spirocyclic quinazolin-4-(1H)-ones, spiro-heterocyclic quinazolin-4-(1H)-ones, and dispirocyclic quinazolin-4-(1H)-ones, is described, which results in high yields by using ionic liquids as green media. The method involves the reaction of 2-aminobenzamides with various ketones catalyzed by iodine and provides new quinazolin-4-(1H)-one library for biomedical screening.
The zero-voltage-switching converter connected with the battery and the solar cell as DC sources (2002)

This paper presents a new type of power converter with which a battery and a solar cell are connected. The circuit cannot only track the maximum peak power from the solar cell but also charge and discharge the battery. This circuit obtains the maximum power of the solar cell, under conditions from light to heavy load. In addition, all switches in the circuit can achieve zero-voltage-switching (ZVS). This paper describes the analytical results of the new power converter by applying the state space averaging method and also shows the range in which the circuit can operate under ZVS. It is noted that the experimental values agree well with the theoretical results.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Cross-feature analysis for detecting ad-hoc routing anomalies (2004)

With the proliferation of wireless devices, mobile ad-hoc networking (MANET) has become a very exciting and important technology. However, MANET is more vulnerable than wired networking. Existing security mechanisms designed for wired networks have to be redesigned in this new environment. In this paper, we discuss the problem of intrusion detection in MANET. The focus of our research is on techniques for automatically constructing anomaly detection models that are capable of detecting new (or unseen) attacks. We introduce a new data mining method that performs "cross-feature analysis" to capture the inter-feature correlation patterns in normal traffic. These patterns can be used as normal profiles to detect deviation (or anomalies) caused by attacks. We have implemented our method on a few well known ad-hoc routing protocols, namely, Dynamic Source Routing (DSR) and Ad-hoc On-Demand Distance Vector (AODV), and have conducted extensive experiments on the ns-2 simulator. The results show that the anomaly detection models automatically computed using our data mining method can effectively, detect anomalies caused by typical routing intrusions.
The Sko1p Repressor and Gcn4p Activator Antagonistically Modulate Stress-Regulated Transcription in <i>Saccharomyces cerevisiae</i> (2001)

In the transcriptional response of Saccharomyces cerevisiae to stress, both activators and repressors are implicated. Here we demonstrate that the ion homeostasis determinant, HAL1, is regulated by two antagonistically operating bZIP transcription factors, the Sko1p repressor and the Gcn4p activator. A single CRE-like sequence (CRE(HAL1)) at position -222 to -215 with the palindromic core sequence TTACGTAA is essential for stress-induced expression of HAL1. Down-regulation of HAL1 under normal growth conditions requires specific binding of Sko1p to CRE(HAL1) and the corepressor gene SSN6. Release from this repression depends on the function of the high-osmolarity glycerol pathway. The Gcn4p transcriptional activator binds in vitro to the same CRE(HAL1) and is necessary for up-regulated HAL1 expression in vivo, indicating a dual control mechanism by a repressor-activator pair occupying the same promoter target sequence. gcn4 mutants display a strong sensitivity to elevated K(+) or Na(+) concentrations in the growth medium. In addition to reduced HAL1 expression, this sensitivity is explained by the fact that amino acid uptake is drastically impaired by high Na(+) and K(+) concentrations in wild-type yeast cells. The reduced amino acid biosynthesis of gcn4 mutants would result in amino acid deprivation. Together with the induction of HAL1 by amino acid starvation, these results suggest that salt stress and amino acid availability are physiologically interconnected.
Anytime Lane-Level Intersection Estimation Based on Trajectories of Other Traffic Participants (2019)

Estimating and understanding the current scene is an inevitable capability of automated vehicles. Usually, maps are used as prior for interpreting sensor measurements in order to drive safely and comfortably. Only few approaches take into account that maps might be outdated and lead to wrong assumptions on the environment. This work estimates a lane-level intersection topology without any map prior by observing the trajectories of other traffic participants.We are able to deliver both a coarse lane-level topology as well as the lane course inside and outside of the intersection using Markov chain Monte Carlo sampling. The model is neither limited to a number of lanes or arms nor to the topology of the intersection.We present our results on an evaluation set of 1000 simulated intersections and achieve 99.9% accuracy on the topology estimation that takes only 36ms, when utilizing tracked object detections. The precise lane course on these intersections is estimated with an error of 15cm on average after 140ms. Our approach shows a similar level of precision on 14 real-world intersections with 18cm average deviation on simple intersections and 27cm for more complex scenarios. Here the estimation takes only 113ms in total.
Thread detection in dynamic text message streams (2006)

Text message stream is a newly emerging type of Web data which is produced in enormous quantities with the popularity of Instant Messaging and Internet Relay Chat. It is beneficial for detecting the threads contained in the text stream for various applications, including information retrieval, expert recognition and even crime prevention. Despite its importance, not much research has been conducted so far on this problem due to the characteristics of the data in which the messages are usually very short and incomplete. In this paper, we present a stringent definition of the thread detection task and our preliminary solution to it. We propose three variations of a single-pass clustering algorithm for exploiting the temporal information in the streams. An algorithm based on linguistic features is also put forward to exploit the discourse structure information. We conducted several experiments to compare our approaches with some existing algorithms on a real dataset. The results show that all three variations of the single-pass algorithm outperform the basic single-pass algorithm. Our proposed algorithm based on linguistic features improves the performance relatively by 69.5% and 9.7% when compared with the basic single-pass algorithm and the best variation algorithm in terms of F1 respectively.
Purification and Physical Characterization of Tyrosyl Ribonucleic Acid Synthetases from Escherichia coli and Bacillus subtilis<sup>*</sup> (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification and Physical Characterization of Tyrosyl Ribonucleic Acid Synthetases from Escherichia coli and Bacillus subtilis*Richard Calendar and Paul BergCite this: Biochemistry 1966, 5, 5, 1681–1690Publication Date (Print):May 1, 1966Publication History Published online1 May 2002Published inissue 1 May 1966https://doi.org/10.1021/bi00869a033RIGHTS & PERMISSIONSArticle Views317Altmetric-Citations177LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (3 MB) Get e-Alerts Get e-Alerts
Light-trapping for room temperature Bose-Einstein condensation in InGaAs quantum wells (2016)

We demonstrate the possibility of room-temperature, thermal equilibrium Bose-Einstein condensation (BEC) of exciton-polaritons in a multiple quantum well (QW) system composed of InGaAs quantum wells surrounded by InP barriers, allowing for the emission of light near telecommunication wavelengths. The QWs are embedded in a cavity consisting of double slanted pore (SP2) photonic crystals composed of InP. We consider exciton-polaritons that result from the strong coupling between the multiple quantum well excitons and photons in the lowest planar guided mode within the photonic band gap (PBG) of the photonic crystal cavity. The collective coupling of three QWs results in a vacuum Rabi splitting of 3% of the bare exciton recombination energy. Due to the full three-dimensional PBG exhibited by the SP2 photonic crystal (16% gap to mid-gap frequency ratio), the radiative decay of polaritons is eliminated in all directions. Due to the short exciton-phonon scattering time in InGaAs quantum wells of 0.5 ps and the exciton non-radiative decay time of 200 ps at room temperature, polaritons can achieve thermal equilibrium with the host lattice to form an equilibrium BEC. Using a SP2 photonic crystal with a lattice constant of a = 516 nm, a unit cell height of 2a=730nm and a pore radius of 0.305a = 157 nm, light in the lowest planar guided mode is strongly localized in the central slab layer. The central slab layer consists of 3 nm InGaAs quantum wells with 7 nm InP barriers, in which excitons have a recombination energy of 0.944 eV, a binding energy of 7 meV and a Bohr radius of aB = 10 nm. We take the exciton recombination energy to be detuned 35 meV above the lowest guided photonic mode so that an exciton-polariton has a photonic fraction of approximately 97% per QW. This increases the energy range of small-effective-mass photonlike states and increases the critical temperature for the onset of a Bose-Einstein condensate. With three quantum wells in the central slab layer, the strong light confinement results in light-matter coupling strength of ℏΩ = 13.7 meV. Assuming an exciton density per QW of (15aB)<sup>-2</sup>, well below the saturation density, in a 2-D box-trap with a side length of 10 to 500 µm, we predict thermal equilibrium Bose-Einstein condensation well above room temperature.
The Fortran I compiler (2000)

The Fortran I compiler was the first demonstration that it is possible to automatically generate efficient machine code from high-level languages. It has thus been enormously influential. The article presents a brief description of the techniques used in the Fortran I compiler for the parsing of expressions, loop optimization, and register allocation.
Deceleration of silicon etch rate at high aspect ratios (2000)

The molecular-flow conductance of a high aspect ratio feature can limit the etching species arriving at the bottom of the feature and thus limit the etch rate. A simple conductance model can predict the etch rate of the time-domain multiplexed etch process with good results at moderate-aspect ratios (5–20) for trenches, but at very high aspect ratios (&gt;20) the conductance model breaks down. Other mechanisms are needed to explain the deceleration of etch rate and almost complete etch stop. In this article the reasons for etch stop at the bottom of deep features are discussed. Measurement results of deep silicon etching are presented. Very deep holes and trenches were etched into silicon to study the effect of process parameters. At moderate aspect ratios the bottom of the hole is nearly flat and the sidewalls are vertical. At high aspect ratio the sidewalls start to bow and the feature bottom turns into a sharp spearhead. The shape of the feature can have an impact on the step coverage of the passivation layer deposition during the passivation step and on passivation removal during the etch step, leading to excessive sidewall etching and reduced etch rate at the feature bottom. The nonzero sidewall reaction probability and flow conductance of tapered tubes were studied by Monte Carlo simulation. The main reason for deceleration of etching seems to be the loss of etchant species due to sidewall reactions combined with feature closure by ion-limited passivation polymer etching.
Human pluripotent stem cell–derived brain pericyte–like cells induce blood-brain barrier properties (2019)

Brain pericytes play important roles in the formation and maintenance of the neurovascular unit (NVU), and their dysfunction has been implicated in central nervous system disorders. While human pluripotent stem cells (hPSCs) have been used to model other NVU cell types, including brain microvascular endothelial cells (BMECs), astrocytes, and neurons, hPSC-derived brain pericyte-like cells have not been integrated into these models. In this study, we generated neural crest stem cells (NCSCs), the embryonic precursor to forebrain pericytes, from hPSCs and subsequently differentiated NCSCs to brain pericyte-like cells. These cells closely resembled primary human brain pericytes and self-assembled with endothelial cells. The brain pericyte-like cells induced blood-brain barrier properties in BMECs, including barrier enhancement and reduced transcytosis. Last, brain pericyte-like cells were incorporated with iPSC-derived BMECs, astrocytes, and neurons to form an isogenic human model that should prove useful for the study of the NVU.
The Homoleptic Sandwich Anion [Co(P<sub>2</sub>C<sub>2</sub><i>t</i>Bu<sub>2</sub>)<sub>2</sub>]<sup>−</sup>: A Versatile Building Block for Phosphaorganometallic Chemistry (2008)

A sandwich recipe: Treatment of the anthracene complex [Co(η4-C14H10)2]− with tBuCP leads to the new diphosphacyclobutadiene sandwich anion 1, which can be converted into the unprecedented phosphaorganometallic complexes 2–4 upon treatment with different electrophiles (see scheme). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z800813_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
High-frequency percussive ventilation (2005)

High-frequency percussive ventilation has been shown to provide favorable gas exchange in several well-defined patient populations. It reliably improves oxygenation and provides adequate ventilation at lower peak pressures than conventional ventilation. Adequately powered, randomized, prospective studies demonstrating significant mortality benefit have not yet been performed.