gebhart/patrepeval huggingface dataset:
experimentally induced asthma and alter pulmonary dendritic cell phenotype and function (2005)

The role of natural CD4 � CD25 � regulatory T (T reg) cells in the control of allergic asthma remains poorly understood. We explore the impact of T reg cell depletion on the allergic response in mice susceptible (A/J) or comparatively resistant (C3H) to the development of allergen-induced airway hyperresponsiveness (AHR). In C3H mice, anti-CD25‐mediated T reg cell depletion before house dust mite treatment increased several features of the allergic diathesis (AHR, eosinophilia, and IgE), which was concomitant with elevated T helper type 2 (Th2) cytokine production. In similarly T reg cell‐depleted A/J mice, we observed a moderate increase in airway eosinophilia but no effects on AHR, IgE levels, or Th2 cytokine synthesis. As our experiments suggested that T reg cell depletion in C3H mice before sensitization was sufficient to enhance the allergic phenotype, we characterized dendritic cells (DCs) in T reg cell‐depleted C3H mice. T reg cell‐depleted mice had increased numbers of pulmonary myeloid DCs with elevated expression of major histocompatibility complex class II, CD80, and CD86. Moreover, DCs from T reg cell‐depleted mice demonstrated an increased capacity to stimulate T cell proliferation and Th2 cytokine production, which was concomitant with reduced IL-12 expression. These data suggest that resistance to allergen-driven AHR is mediated in part by CD4
Definition of a Novel Feed-Forward Mechanism for Glycolysis-HIF1α Signaling in Hypoxic Tumors Highlights Aldolase A as a Therapeutic Target (2016)

The hypoxia-inducible transcription factor HIF1α drives expression of many glycolytic enzymes. Here, we show that hypoxic glycolysis, in turn, increases HIF1α transcriptional activity and stimulates tumor growth, revealing a novel feed-forward mechanism of glycolysis-HIF1α signaling. Negative regulation of HIF1α by AMPK1 is bypassed in hypoxic cells, due to ATP elevation by increased glycolysis, thereby preventing phosphorylation and inactivation of the HIF1α transcriptional coactivator p300. Notably, of the HIF1α-activated glycolytic enzymes we evaluated by gene silencing, aldolase A (ALDOA) blockade produced the most robust decrease in glycolysis, HIF-1 activity, and cancer cell proliferation. Furthermore, either RNAi-mediated silencing of ALDOA or systemic treatment with a specific small-molecule inhibitor of aldolase A was sufficient to increase overall survival in a xenograft model of metastatic breast cancer. In establishing a novel glycolysis-HIF-1α feed-forward mechanism in hypoxic tumor cells, our results also provide a preclinical rationale to develop aldolase A inhibitors as a generalized strategy to treat intractable hypoxic cancer cells found widely in most solid tumors. Cancer Res; 76(14); 4259-69. ©2016 AACR.
Medial temporal lobe atrophy predicts Alzheimer's disease in patients with minor cognitive impairment. (2002)

The ability to detect patients at high risk for Alzheimer type dementia among those with minor cognitive impairment increases when data on age and memory function are combined with measures of medial temporal lobe atrophy. Volumetry of the hippocampus is preferred, but qualitative rating of medial temporal lobe atrophy is a good alternative.
Protective actions of carbocromene against amitriptyline-induced cardiotoxicity in anesthetized rats. (1986)

This study was designed to analyze the effects of carbocromene on circulatory and electrophysiologic failure in rats anesthetized with urethane. Amitriptyline was infused at 0.4 mg/kg/min (n = 12) until death of the animals at 30 +/- 6.7 min due to vascular collapse. Following initial heart rate increase and QRS prolongation, this was characterized by progressive hypotension, bradycardia, intraventricular conduction delay, S-T segment elevation and A-V block. In the initial 31 rats, carbocromene was administered i.v. with 4 mg/kg followed in two groups by 40 (n = 13) and 80 micrograms/kg/min (n = 18) i.v., respectively. The drug was effective in protecting the animals against the acute cardiovascular failure induced by amitriptyline. The preservation was associated with a diminution of the hypotension, negative chronotropic actions, S-T segment elevation and the incidence of A-V block produced by amitriptyline. Survival time of the rats increased to 46 +/- 5.3 min (p less than 0.05 vs. amitriptyline controls) and 63 +/- 6.5 min (p less than 0.01) with infusion of 40 and 80 micrograms/kg/min carbocromene, respectively. These results suggest that carbocromene is an effective treatment of amitriptyline-induced cardiotoxicity by massive overdose with beneficial effects largely due to hemodynamic and metabolic oxygen-sparing impact on the heart. Membrane stabilizing antiarrhythmic carbocromene effects may be contributory factors in its protective value in the treatment of acute amitriptyline poisoning.
Effects of depletion exercise and light training on muscle glycogen supercompensation in men (2003)

Supercompensated muscle glycogen can be achieved by using several carbohydrate (CHO)-loading protocols. This study compared the effectiveness of two "modified" CHO-loading protocols. Additionally, we determined the effect of light cycle training on muscle glycogen. Subjects completed a depletion (D, n = 15) or nondepletion (ND, n = 10) CHO-loading protocol. After a 2-day adaptation period in a metabolic ward, the D group performed a 120-min cycle exercise at 65% peak oxygen uptake (VO2 peak) followed by 1-min sprints at 120% VO2 peak to exhaustion. The ND group performed only 20-min cycle exercise at 65% VO2 peak. For the next 6 days, both groups ate the same high-CHO diets and performed 20-min daily cycle exercise at 65% VO2 peak followed by a CHO beverage (105 g of CHO). Muscle glycogen concentrations of the vastus lateralis were measured daily with 13C magnetic resonance spectroscopy. On the morning of day 5, muscle glycogen concentrations had increased 1.45 (D) and 1.24 (ND) times baseline (P < 0.001) but did not differ significantly between groups. However, on day 7, muscle glycogen of the D group was significantly greater (p < 0.01) than that of the ND group (130 +/- 7 vs. 104 +/- 5 mmol/l). Daily cycle exercise decreased muscle glycogen by 10 +/- 2 (D) and 14 +/- 5 mmol/l (ND), but muscle glycogen was equal to or greater than preexercise values 24 h later. In conclusion, a CHO-loading protocol that begins with a glycogen-depleting exercise results in significantly greater muscle glycogen that persists longer than a CHO-loading protocol using only an exercise taper. Daily exercise at 65% VO2 peak for 20 min can be performed throughout the CHO-loading protocol without negatively affecting muscle glycogen supercompensation.
Dependent Randomized Rounding via Exchange Properties of Combinatorial Structures (2010)

We consider the problem of randomly rounding a fractional solution x in an integer polytope P ⊆ [0,1] <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">n</sup> to a vertex X of P, so that E[X] = x. Our goal is to achieve concentration properties for linear and submodular functions of the rounded solution. Such dependent rounding techniques, with concentration bounds for linear functions, have been developed in the past for two poly topes: the assignment poly tope (that is, bipartite matchings and 6-matchings) [32], [19], [23], and more recently for the spanning tree poly tope [2]. These schemes have led to a number of new algorithmic results. In this paper we describe a new swap rounding technique which can be applied in a variety of settings including matroids and matroid intersection, while providing Chernoff-type concentration bounds for linear and submodular functions of the rounded solution. In addition to existing techniques based on negative correlation, we use a martingale argument to obtain an exponential tail estimate for monotone submodular functions. The rounding scheme explicitly exploits exchange properties of the underlying combinatorial structures, and highlights these properties as the basis for concentration bounds. Matroids and matroid intersection provide a unifying framework for several known applications [19], [23], [7], [22], [2] as well as new ones, and their generality allows a richer set of constraints to be incorporated easily. We give some illustrative examples, with a more comprehensive discussion deferred to a later version of the paper.
Water-absorbing characteristics of acrylic acid-grafted carboxymethyl cellulose synthesized by photografting (1996)

Cellulosic absorbents for water were synthesized by photografting (λ > 300 nm) acrylic acid (AA) onto fiberous carboxymethyl cellulose (CMC, degree of substitution [DS] = 0.1–0.4) at 30°C in the presence of N,N′-methylenebisacrylamide as a crosslinker. The CMC sample was pretreated with hydrogen peroxide in the presence of sulfuric acid to prepare CMC peroxides with a peracid type as a polymeric photoinitiator. The peroxides were active for the photografting and AA could be grafted onto CMC with percent graftings higher than 150% by photoirradiation of 10 min at 30°C. The amount of water absorbed increased with increasing percent grafting of AA and DS of CMC. The amount was reduced considerably when the absorbents were prepared by the photografting of AA onto crosslinked CMC in the absence of the crosslinker. Graft copolymers which display a decreasing water absorbency as a function of temperature were prepared by two methods: In the first synthesis method, AA and N-isopropylacrylamide (NIPAAm) binary monomers were graft-copoly-merized onto CMC samples using photoinitiation. In the second method, photografting of AA was followed by a second-step photografting of NIPAAm to produce a terpolymer with two types of side chains of differing repeat unit composition on the CMC substrate. Graft copolymers formed by both methods showed decreasing water absorbency as temperature increased with losses in water absorbency of up to about 60% as the temperature was increased above 30°C. Effects of NIPAAm composition and corsslinker content in the graft copolymers on the decreasing water absorbency as a function of temperature were also examined. © 1996 John Wiley & Sons, Inc.
The chromosome translocation (11;14)(p13;q11) associated with T cell acute leukemia. Asymmetric diversification of the translocational junctions. (1990)

The t(11;14)(p13;q13) translocation associated with T cell acute lymphocytic leukemia generates two abnormal chromosomes, designated 11p+ and 14q-. To investigate the mechanism of t(11;14)(p13;q11) formation, we analyzed the translocation junctions of 11p+ and 14q- from two patients. The 11p+ junctions consisted of precise fusions of a pseudo recombination signal from chromosome 11 and the downstream recombination signal of the TCR D delta 2 gene segment from chromosome 14. In contrast, the 14q- junctions from both patients were diversified by random loss and addition of nucleotides at the translocation site. This asymmetric pattern of junctional diversification is typical of normal Ig/TCR gene rearrangement, and therefore implies that the t(11;14)(p13;q11) translocation arose due to aberrant activity of the Ig/TCR recombinase.
Mouse pathogenicity studies of Nocardia asteroides complex species and clinical correlation with human isolates (1993)

Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified on the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organisms were derived showed that isolates derived from blood, brain, or bone marrow were more likely to be N. farcinica than the other two species. A study of the virulence of ten strains of each species was undertaken, using a mouse model with intravenous inoculation. The 50% lethal doses (LD50) for N. farcinica were significantly lower than those of the other two species. LD50 values for N. nova and N. asteroides were not significantly different. The above data confirming the greater virulence of N. farcinica support the identification of species within the N. asteroides complex.
STUDIES ON THE GENERALIZED SHWARTZMAN REACTION (1952)

Certain factors involved in the production of the generalized Shwartzman reaction with meningococcal toxin in rabbits were investigated. The optimal amounts of toxin for the preparing and provoking injections, and the optimal time interval between injections were determined. Under suitable conditions of dosage and timing, bilateral cortical necrosis of the kidneys was produced in a high proportion of animals. When excessive amounts of toxin were used for preparation the incidence of the reaction was reduced. Animals undergoing the generalized Shwartzman reaction became severely prostrated within several hours after the provoking injection of toxin. The renal lesion became fully developed within 24 hours, and its occurrence was associated with a rise of the blood non-protein nitrogen. Edema and petechial hemorrhages in the ears were observed in rabbits with advanced renal lesions. The earliest change in the kidneys in the generalized Shwartzman reaction was the appearance of homogeneous, eosinophilic material, resembling fibrinoid, within the lumen of the glomerular capillaries. Occlusion of the capillaries by this material was regarded as the cause of subsequent tubular necrosis in the renal cortex. The material appeared to be derived from the blood, rather than from the capillary walls. Cortisone enhanced the lethal effect of a single, large dose of meningococcal toxin, as well as causing bilateral renal cortical necrosis. The generalized Shwartzman reaction produced by two injections of toxin was aggravated by cortisone and ACTH. Profound polymorphonuclear leukopenia was produced by both the preparing and provoking injections of toxin. When leukopenia was produced before the preparing injection of toxin, by treatment with nitrogen mustard, the generalized Shwartzman reaction was inhibited. During the intervals before and after leukopenia, and when leukopenia was prevented by shielding the femoral bone marrow from the action of nitrogen mustard, no inhibition of the generalized Shwartzman phenomenon was demonstrable. Various colloidal and particulate materials, which are capable of provoking the local skin Shwartzman reaction when injected intravenously, failed to provoke the generalized Shwartzman reaction. A working hypothesis was set up to account for certain events in the generalized Shwartzman reaction.
Glycerol 1,2-cyclic phosphate in centric diatoms. Observation by 31P NMR in vivo, isolation, and structural determination. (1987)

We have isolated and identified glycerol 1,2-cyclic phosphate as the compound responsible for a unique prominent resonance at 19.1 ppm in the 31P NMR spectrum in vivo of four species of centric diatoms, where it is responsible for 15 to 30% of the total signals from organophosphates. This appears to be the first observation of this compound as a major metabolite. It was not detectable in nine other species of algae, including four species of pennate diatoms.
Non-display bio-optic applications of liquid crystals (2011)

Liquid crystal (LC) devices have been largely developed for LC displays and in the last decade for optical telecommunications; however, their application in the field of optical imaging is just starting to emerge. They can be miniaturised and thus have a great potential to be used with miniature optical imaging systems for biomedical applications. LC devices specifically designed for integration into biomedical optical imaging systems are reviewed. Using a combination of a few LC retarders, spectropolarimetric imaging of tissue can be obtained. LC tunable filters with high dynamic range and large throughput are designed for hyperspectral imaging and for spectral domain optical coherence tomography. The designs are based on several concepts, both using the classical stack of retarders and using more modern designs based on single layer in a Fabry–Perot cavity, in a resonant waveguide structure or in a photonic crystal.
TaintTrace: Efficient Flow Tracing with Dynamic Binary Rewriting (2006)

TaintTrace is a high performance flow tracing tool that protects systems against security exploits. It is based on dynamic execution binary rewriting empowering our tool with fine-grained monitoring of system activities such as the tracking of the usage and propagation of data originated from the network. The challenge lies in minimizing the run-time overhead of the tool. TaintTrace uses a number of techniques such as direct memory mapping to optimize performance. In this paper, we demonstrate that TaintTrace is effective in protecting against various attacks while maintaining a modest slowdown of 5.5 times, offering significant improvements over similar tools.
The Fluorescent-Oil Film Method and Other Techniques for Boundary-Layer Flow Visualization (1959)

A flow-visualization technique, known as the fluorescent-oil film method, has been developed which appears to be generally simpler and to require less experience and development of technique than previously published methods. The method is especially adapted to use in the large high-powered wind tunnels which require considerable time to reach the desired test conditions. The method consists of smearing a film of fluorescent oil over a surface and observing where the thickness is affected by the shearing action of the boundary layer. These films are detected and identified, and their relative thicknesses are determined by use of ultraviolet light. Examples are given of the use of this technique. Other methods that show promise in the study of boundary-layer conditions are described. These methods include the use of a temperature-sensitive fluorescent paint and the use of a radiometer that is sensitive to the heat radiation from a surface. Some attention is also given to methods that can be used with a spray apparatus in front of the test model.
Self-correlating predictive information tracking for large-scale production systems (2009)

Automatic management of large-scale production systems requires a continuous monitoring service to keep track of the states of the managed system. However, it is challenging to achieve both scalability and high information precision while continuously monitoring a large amount of distributed and time-varying metrics in large-scale production systems. In this paper, we present a new self-correlating, predictive information tracking system called InfoTrack, which employs lightweight temporal and spatial correlation discovery methods to minimize continuous monitoring cost. InfoTrack combines both metric value prediction within individual nodes and adaptive clustering among distributed nodes to suppress remote information update in distributed system monitoring. We have implemented a prototype of the InfoTrack system and deployed the system on the PlanetLab. We evaluated the performance of the InfoTrack system using both real system traces and micro-benchmark prototype experiments. The experimental results show that InfoTrack can reduce the continuous monitoring cost by 50-90% while maintaining high information precision (i.e., within 0.01-0.05 error bound).
N-<scp>d</scp>-Biotinyl-7-amino-4-methylcoumarin as a Novel Fluorigenic Substrate for the Determination of Biotinidase Activity (1997)

Abstract A new fluorigenic substrate for the determination of biotinidase, N-d-biotinyl-7-amino-4-methylcoumarin has been synthesized by using BOP reagent. This substrate was used for measuring the enzyme activity in plasma of normal and biotinidase deficient patients. The methodology used here should be applicable to other biological fluids and homogenates to detect biotinidase deficiency.
An electrically pre-equalized 10-Gb/s duobinary transmission system (2005)

Duobinary signaling is combined with a proposed electrical pre-equalization scheme to extend the reach of 10-Gb/s signals that are transmitted over standard single-mode fiber. The proposed scheme is based on predistorting the duobinary signal using two T/2-spaced finite-impulse response (FIR) filters. The outputs of the FIR filters then modulate two optical carriers that are in phase quadrature. Simulation results show that distances in excess of 400 km at bit-error rates less than 10/sup -15/ are possible. Incorporating a forward-error correction scheme can extend the reach to distances in excess of 800 km. The reach limitation arises not from chromatic dispersion but from fiber nonlinearity, relative intensity noise due to phase-modulation-to-amplitude-modulation noise conversion, and optical amplifier noise accumulation. To demonstrate the feasibility of implementing the proposed scheme, a test chip is implemented in a 0.5-/spl mu/m SiGe BiCMOS technology. The chip incorporates two 10-tap T/2-spaced FIR filters, which are sufficient to equalize a 10-Gb/s duobinary signal that is transmitted over distances in excess of 400 km. The pre-equalization capabilities of the chip are tested by postprocessing the measured chip output to mimic the effects of the optical channel.
Banking Stem Cells from Human Exfoliated Deciduous Teeth (SHED): Saving for the Future (2009)

Tooth derived cells are readily accessible and provide an easy and minimally invasive way to obtain and store stem cells for future use. Banking ones own tooth-derived stem cells is a reasonable and simple alternative to harvesting stem cells from other tissues. Obtaining stem cells from human exfoliated deciduous teeth (SHED) is simple and convenient, with little or no trauma. Every child loses primary teeth, which creates the perfect opportunity to recover and store this convenient source of stem cells--should they be needed to treat future injuries or ailments and presents a far better alternative to simply discarding the teeth or storing them as mementos from the past. Furthermore, using ones own stem cells poses few, if any, risks for developing immune reactions or rejection following transplantation and also eliminates the potential of contracting disease from donor cells. Stem cells can also be recovered from developing wisdom teeth and permanent teeth. Individuals have different opportunities at different stages of their life to bank these valuable cells. It is best to recover stem cells when a child is young and healthy and the cells are strong and proliferative. The purpose of this review is to discuss the present scenario as well as the technical details of tooth banking as related to SHED cells.
The 3′-Terminal Sequence of <i>Escherichia coli</i> 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites (1974)

With a stepwise degradation and terminal labeling procedure the 3'-terminal sequence of E. coli 16S ribosomal RNA is shown to be Pyd-A-C-C-U-C-C-U-U-A(OH). It is suggested that this region of the RNA is able to interact with mRNA and that the 3'-terminal U-U-A(OH) is involved in the termination of protein synthesis through base-pairing with terminator codons. The sequence A-C-C-U-C-C could recognize a conserved sequence found in the ribosome binding sites of various coliphage mRNAs; it may thus be involved in the formation of the mRNA.30S subunit complex.