gebhart/patrepeval huggingface dataset:
siRNA for Influenza Therapy (2010)

Influenza virus is one of the most prevalent and ancient infections in humans. About a fifth of world's population is infected by influenza virus annually, leading to high morbidity and mortality, particularly in infants, the elderly and the immunocompromised. In the US alone, influenza outbreaks lead to roughly 30,000 deaths each year. Current vaccines and anti-influenza drugs are of limited use due to high mutation rate of the virus and side effects. In recent years, RNA interference, triggered by synthetic short interfering RNA (siRNA), has rapidly evolved as a potent antiviral regimen. Properly designed siRNAs have been shown to function as potent inhibitors of influenza virus replication. The siRNAs outperform traditional small molecule antivirals in a number of areas, such as ease of design, modest cost, and fast turnaround. Although specificity and tissue delivery remain major bottlenecks in the clinical applications of RNAi in general, intranasal application of siRNA against respiratory viruses including, but not limited to influenza virus, has experienced significant success and optimism, which is reviewed here.
Lipopeptide Production in <i>Pseudomonas</i> sp. Strain DSS73 Is Regulated by Components of Sugar Beet Seed Exudate via the Gac Two-Component Regulatory System (2002)

Pseudomonas sp. strain DSS73 isolated from the sugar beet rhizosphere produces the cyclic lipopeptide amphisin, which inhibits the growth of plant-pathogenic fungi. By Tn5::luxAB mutagenesis, we obtained two nonproducing mutant strains, DSS73-15C2 and DSS73-12H8. The gene interrupted by the transposon in strain DSS73-15C2 (amsY) encoded a protein with homology to peptide synthetases that was designated amphisin synthetase. DSS73-12H8 carried the transposon in a regulatory gene encoding a protein with homology to the sensor kinase GacS. Growth of strain DSS73-15C2 (amsY) was impaired during the transition to stationary phase in a minimal medium amended with an exudate of sugar beet seeds. This growth phenotype could be complemented by purified amphisin. Seed exudate further induced expression of bioluminescence from the amsY::luxAB reporter during the transition to stationary phase. This agreed with an increase in amphisin production by the DSS73 wild-type strain during early stationary phase. Amphisin synthesis in DSS73 was strictly dependent on GacS, and even induction by seed exudate depended on a functional gacS locus. Hence, a signal triggering the GacS/GacA two-component system appeared to be present in the seed exudate.
Angiotensin receptor blockade attenuates cigarette smoke–induced lung injury and rescues lung architecture in mice (2011)

Chronic obstructive pulmonary disease (COPD) is a prevalent smoking-related disease for which no disease-altering therapies currently exist. As dysregulated TGF-β signaling associates with lung pathology in patients with COPD and in animal models of lung injury induced by chronic exposure to cigarette smoke (CS), we postulated that inhibiting TGF-β signaling would protect against CS-induced lung injury. We first confirmed that TGF-β signaling was induced in the lungs of mice chronically exposed to CS as well as in COPD patient samples. Importantly, key pathological features of smoking-associated lung disease in patients, e.g., alveolar injury with overt emphysema and airway epithelial hyperplasia with fibrosis, accompanied CS-induced alveolar cell apoptosis caused by enhanced TGF-β signaling in CS-exposed mice. Systemic administration of a TGF-β-specific neutralizing antibody normalized TGF-β signaling and alveolar cell death, conferring improved lung architecture and lung mechanics in CS-exposed mice. Use of losartan, an angiotensin receptor type 1 blocker used widely in the clinic and known to antagonize TGF-β signaling, also improved oxidative stress, inflammation, metalloprotease activation and elastin remodeling. These data support our hypothesis that inhibition of TGF-β signaling through angiotensin receptor blockade can attenuate CS-induced lung injury in an established murine model. More importantly, our findings provide a preclinical platform for the development of other TGF-β-targeted therapies for patients with COPD.
Leveraging shape and depth in user authentication from in-air hand gestures (2015)

Depth-sensors, such as the Kinect, have predominately been used as a gesture recognition device. Recent works, however, have proposed using these sensors for user authentication using biometric modalities such as: face, speech, gait and gesture. The last of these modalities - gestures, used in the context of full-body and hand-based gestures, is relatively new but has shown promising authentication performance. In this paper, we focus on hand-based gestures that are performed in-air. We present a novel approach to user authentication from such gestures by leveraging a temporal hierarchy of depth-aware silhouette covariances. Further, we investigate the usefulness of shape and depth information in this modality, as well as the importance of hand movement when performing a gesture. By exploiting both shape and depth information our method attains an average 1.92% Equal Error Rate (EER) on a dataset of 21 users across 4 predefined hand-gestures. Our method consistently outperforms related methods on this dataset.
Epidermal growth factor receptor activation and inhibition in 3<scp>D </scp><i>in vitro</i> models of normal skin and human cutaneous squamous cell carcinoma (2012)

The transmembrane tyrosine kinase epidermal growth factor receptor (EGFR) is considered a key player in the development of cutaneous squamous cell carcinoma (SCC), which is the second most common malignancy in white populations. Inhibition of EGFR with the small molecule tyrosine kinase inhibitor erlotinib is currently under clinical investigation in cutaneous SCC patients. In this study, we investigated the effects of EGFR activation and inhibition on normal and malignant in vitro human skin equivalents (HSEs). In healthy HSEs, increasing EGF concentrations ranging from 5 to 50 ng/mL resulted in a dramatic decrease in epidermal proliferation as immunohistochemically assessed by Ki67 and increased epidermal stress as assessed by K17 after 2 weeks of air-exposed culture. Also, higher concentrations of EGF induced remarkable epidermal disorganization with loss of proper stratification. Similar effects were observed in HSEs generated with cutaneous SCC cell lines SCC-12B2 and SCC-13. Treatment of both healthy and SCC-HSEs with 10 μM erlotinib resulted in efficient reduction of epidermal thickness from 10 to 3 viable cell layers and counteracted EGF-induced epidermal stress. Remarkably, erlotinib treatment caused severe desquamation in healthy HSEs, reminiscent of xerosis as a known side-effect in patients treated with erlotinib. The presented three-dimensional organotypic SCC models appear suitable for further investigations on the morphological and functional impacts of modifying EGFR signaling in cutaneous SCC, without burdening patients or mice. The effective inhibition of epidermal growth by erlotinib in our HSEs confirms the therapeutic potential of this tyrosine kinase inhibitor for cutaneous SCC patients.
Racemische und optisch aktive 2‐Chlorethylcarbamoyl‐Derivate des 7,8‐Dimethoxy‐1‐phenyl‐1<i>H</i>‐3‐benzazepins: Neue Strukturtypen von DA, NE und 5‐HT Uptake Inhibitoren (1990)

The racemic and optically active 2-chloroethylcarbamoyl derivatives of 7,8-dimethoxy-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepines 6 and 8 were synthesized. The enantiomers of the benzazepine 5 were obtained by consecutive treatment of (+/-)-5 with (-) and (+)-dibenzoyl-tartaric acids. Compounds 6 are strong DA and NE uptake inhibitors while 8 exhibits inhibition towards 5-HT. The inhibitory effect of 6 is characterized by clearly expressed enantioselectivity. So 1-S-6 is a 26, resp. a 40 times stronger inhibitor of DA and NE uptake than 1-S-6. (+/-)-6 shows moderate antiulcer activity on water-immersion stress ulcer.
Forced in‐plane flow of an epoxy resin in fibrous networks (1986)

Abstract A method to study the forced in‐plane flow of an epoxy resin into fibrous networks is presented. A radial in‐plane flow apparatus has been developed which allows strict control of all important experimental variables as well as visual observation of the experiment. From the position and shape of the advancing resin front as a function of time, effective directional permeabilities are obtained and used to compare structurally different fibrous networks. Test results for an initial series of woven and nonwoven fabrics reinforce the method of data analysis and demonstrate the effects of pore structure, weave type, weave balance, fabric layering, fiber orientation, and compressibility.
Complement Activation and Intraventricular Rituximab Distribution in Recurrent Central Nervous System Lymphoma (2013)

We provide the first evidence of C3 activation within the neuroaxis with intraventricular immunotherapy and suggest that complement may contribute to immunotherapeutic responses of rituximab in CNS lymphoma. Penetration of rituximab into neural tissue is supported by this pharmacokinetic model and may contribute to efficacy. These findings have general implications for intraventricular immunotherapy. Our data highlight potential innovations to improve efficacy of intraventricular immunotherapy both via modulation of the innate immune response as well as innovations in drug delivery.
Oxygen distribution in the retinal and choroidal vessels of the cat as measured by a new phosphorescence imaging method (1992)

The oxygen tension in the vessels of the retina and optic nerve head has been measured noninvasively with a new phosphorescence imaging method. A phosphorescent oxygen-dependent probe, injected into the bloodstream of cats, was excited with a flash of light and the phosphorescence lifetime of the probe was measured. A simple Stern-Volmer relationship was used to convert lifetime to oxygen tension, and two-dimensional maps of intravascular oxygen tension were produced. We describe the equipment and the methodology for obtaining oxygen maps.
Action of Antifouling Paints. (1946)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAction of Antifouling Paints.John D. Ferry and Gordon A. RileyCite this: Ind. Eng. Chem. 1946, 38, 7, 699–701Publication Date (Print):July 1, 1946Publication History Published online1 May 2002Published inissue 1 July 1946https://pubs.acs.org/doi/10.1021/ie50439a016https://doi.org/10.1021/ie50439a016research-articleACS PublicationsRequest reuse permissionsArticle Views54Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Experiences creating three implementations of the repast agent modeling toolkit (2006)

Many agent-based modeling and simulation researchers and practitioners have called for varying levels of simulation interoperability ranging from shared software architectures to common agent communications languages. These calls have been at least partially answered by several specifications and technologies. In fact, Tanenbaum [1988] has remarked that the “nice thing about standards is that there are so many to choose from.” Tanenbaum goes on to say that “if you do not like any of them, you can just wait for next year's model.” This article does not seek to introduce next year's model. Rather, the goal is to contribute to the larger simulation community the authors' accumulated experiences from developing several implementations of an agent-based simulation toolkit. As such, this article focuses on the implementation of simulation architectures rather than agent communications languages. It is hoped that ongoing architecture standards efforts will benefit from this new knowledge and use it to produce architecture standards with increased robustness.
Biochemical Studies on Oral Toxicity of Ricin. Part III. Interaction of Toxic Lection Ricin with Epithelial Cells of Rat Small Intestine in Vitro. (1992)

To clarify the mechanism of oral toxicity of ricin, the interaction of ricin with the epithelial cells isolated from rat small intestine was compared in vitro with those of other plant lectins by two different determinations, i.e., viability and cytotoxicity. After incubation of the cells for 1 h at 37 degrees C with ricin, B-chain, castor bean hemagglutinin (CBH), soybean agglutinin (SBA), wheat germ agglutinin (WGA), concanavalin A (Con A), and peanut agglutinin (PNA), respectively, followed by staining with trypan blue, ricin and ricin B-chain as well as CBH and SBA were found to have effectively reduced the number of viable cells. On the contrary, only ricin inhibited protein synthesis in the cells and the effect was blocked by D-galactose. Additional experiments employing [125I]-labeled ricin strongly suggested that ricin was first bound via its B-chain to the galactosyl residues on the cell surface followed by internalization into cells as the whole 62 kDa molecule. These results infer first that ricin, as well as other lectins mentioned above, was able to reduce viability of the epithelial cells of rat small intestine by direct binding to the cell surface. The second effect, specific to ricin, was the inhibition of cellular protein synthesis.
Dynamically Controlled Protein Tunneling Paths in Photosynthetic Reaction Centers (2000)

Marcus theory has explained how thermal nuclear motions modulate the energy gap between donor and acceptor sites in protein electron transfer reactions. Thermal motions, however, may also modulate electron tunneling between these reactions. Here we identify a new mechanism of nuclear dynamics amplification that plays a central role when interference among the dominant tunneling pathway tubes is destructive. In these cases, tunneling takes place in protein conformations far from equilibrium that minimize destructive interference. As an example, we demonstrate how this dynamical amplification mechanism affects certain reaction rates in the photosynthetic reaction center and therefore may be critical for biological function.
Quad encoded display (1984)

A colour raster display system based on picture encoding is described. Encoding pictures as a means of data compression has a long history and is a technique of interest to anyone working with complex pictures. Its primary value is the lower bandwidths required of transmission channels, but its more frugal use of store is not to be overlooked. A quad tree as a means of encoding area-coherent pictures is used because it retains spatial information in a form which is still amenable to processing. The combination of this with compression makes this a particularly useful form of coding for raster-style colour pictures, and this paper addresses the problems which had to be overcome in order to construct a display directly exploiting the quad tree and related forms. The potential of such a display for rapidly handling very large amounts of pictorial data and for application to picture-archive retrieval is also discussed. The system has been constructed, is fully operational and incorporates roam and zoom operations. Sample pictures are included.
Behavioural phenotypes of hypomorphic KCC2‐deficient mice (2005)

Hyperpolarizing fast inhibitory neurotransmission by gamma-aminobutyric acid and glycine requires an efficient chloride extrusion mechanism in postsynaptic neurons. A major effector of this task in adult animals is the potassium-chloride co-transporter KCC2 that is selectively and abundantly expressed postsynaptically in most CNS neurons. Yet, the role of KCC2 in adult brain at the systems level is poorly known. Here, we characterize the behaviour of mice doubly heterozygous for KCC2 null and hypomorphic alleles that retain 15-20% of normal KCC2 protein levels in the brain. These hypomorphic KCC2-deficient mice were viable and fertile but weighed 15-20% less than wild-type littermates at 2 weeks old and thereafter. The mice displayed increased anxiety-like behaviour in several tests including elevated plus-maze and were more susceptible to pentylenetetrazole-induced seizures. Moreover, the mice were impaired in water maze learning and showed reduced sensitivity to tactile and noxious thermal stimuli in von Frey hairs, hot plate and tail flick tests. In contrast, the mice showed normal spontaneous locomotor activity in open field and Y-maze tests, and intact motor coordination in rotarod and beam tests. The results suggest that requirements for KCC2-dependent fast hyperpolarizing inhibition may differ among various functional systems of the CNS. As shunting inhibition is expected to be intact in KCC2-deficient neurons, these mice may provide a useful tool to study the specific functions and relative importance of hyperpolarizing fast synaptic inhibition in adult CNS that may have implications for human neuropsychiatric disorders, such as epilepsy, pain and anxiety.
Free curve interpolation using clothoidal curve. (1990)

This paper describes the study on free curve interpolation using Clothoidal curve, which gives a new tool for high-speed continuous path control. Control schemes are proposed for the manipulator to
Process comparison on the microstructure and mechanical properties of fiber-reinforced polyphenylene sulfide using MuCell technology (2018)

The MuCell process is a special injection molding process which utilizes supercritical gas (nitrogen) to create integral foam sandwiches. The advantages are lower weight, higher specific properties and shorter cycle times. In this study, a series of glass fiber-reinforced polyphenylene sulfide foam blanks are manufactured using the MuCell injection molding process. The different variations of the process (low-pressure also known as structural foam injection molding) and high-pressure foam injection molding (also known as “core back expansion,” “breathing mold,” “precision opening,” decompression molding) are used. The sandwich structure and mechanical properties (tensile strength, bending strength, and impact behavior) of the microcellular and glass fiber-reinforced polyphenylene sulfide foams are systematically investigated and compared to compact material. The results showed that the injection parameters (injection speed, foaming mechanism) played an important role in the relative density of microcellular polyphenylene sulfide foams and the mechanical properties. It could be shown that the specific tensile strength decreased while increasing the degree of foaming which can be explained by the increased number of cells and the resulting cell size. This leads to stress peaks which lower the mechanical properties. The Charpy impact strength shows a significant dependence on the fiber orientation. The specific bending modulus of the high-pressure foaming process, however, surpasses the values of the other two processes showing the potential of this manufacturing variation especially with regard to bending loads. Furthermore, a high dependence of the mechanical properties on the fiber orientation of the tested specimens can be found.
A 1 Gb/s deskew IC for RAMBUS automatic test application (2002)

A deskew IC has been designed and fabricated using a 0.5-/spl mu/m GaAs E/D MESFET process. This IC is part of a five-chip chipset used in automatic test equipment for RAMBUS devices and currently shipping in a high-volume. The packaged IC consumes roughly 1/4 the board area and 1/2 the power of the bipolar alternative, allowing the entire high-speed section of a 32-DUT RAMBUS tester to fit in the test head. This paper describes the tester system architecture, circuit design techniques, and measured results of the deskew IC.
SOLO: an MPEG-7 optimum search tool (2002)

Studies surrounding MPEG-7 have so far concentrated only on the normative components. While feature extraction has been an active research subject in the content based retrieval domain, work on proper search engines is scarce. The paper presents SOLO, a perceived MPEG-7 optimum search tool prototype being developed at the University of Sydney. SOLO is built on the meta-search engine and mobile code paradigms, and is equipped with computational intelligence technology.
Novel biochemical pathways of endoglin in vascular cell physiology (2007)

The broad role of the transforming growth factor beta (TGFbeta) signaling pathway in vascular development, homeostasis, and repair is well appreciated. Endoglin is emerging as a novel, complex, and poorly understood regulatory component of the TGFbeta receptor complex, whose importance is underscored by its recognition as the site of mutations causing hereditary hemorrhagic telangiectasia (HHT) [McAllister et al., 1994]. Extensive analyses of endoglin function in normal developmental mouse models [Bourdeau et al., 1999; Li et al., 1999; Arthur et al., 2000] and in HHT animal models [Bourdeau et al., 2000; Torsney et al., 2003] exemplify the importance of understanding endoglin's biochemical functions. However, novel mechanisms underlying the regulation of these pathways continue to emerge. These mechanisms include modification of TGFbeta receptor signaling at the ligand and receptor activation level, direct effects of endoglin on cell adhesion and migration, and emerging roles for endoglin in the determination of stem cell fate and tissue patterning. The purpose of this review is to highlight the cellular and molecular studies that underscore the central role of endoglin in vascular development and disease.
Centrifugal Micromixery (2005)

Abstract We present a modular centrifugal micromixer comprising a mixing unit hosting a planar network of low‐aspect‐ratio microfluidic channels (“disk”), a fixed rotating drive (“player”) and contact‐free dispensers for the continuous feed of educts. The modular setup allows a simple fabrication and a child's play exchange of the mixing unit. High‐speed micromixing is powered by the Coriolis force at volume throughputs of up to milliliters per minute and microchannel! These outstanding characteristics are demonstrated by experiments and accompanying CFD‐simulations.
Homelessness Research: A Consulting Perspective (2008)

The critical questions that need to be addressed by research in homelessness are discussed. The wide range of factors influencing the homelessness research agenda in Australia are highlighted.
The association of CD25 expression on donor CD8+ and CD4+ T cells with graft-versus-host disease after donor lymphocyte infusions. (2005)

Donor CD8 +CD25++ T cells appear to represent a risk factor for GvHD in the DLI setting. Selective depletion of these cells from DLI products may attenuate GvHD without significantly compromising anti-tumor efficacy.