gebhart/patrepeval huggingface dataset:
Inorganic–Organic Thiol–ene Coated Mesh for Oil/Water Separation (2015)

A highly efficient mesh for oil/water separation was fabricated by using a superhydrophobic and superoleophilic coating of thiol-ene hybrid, consisting of pentaerythritol tetra(3-mercaptopropionate) (PETMP), 2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane (TMTVSi), and hydrophobic fumed silica nanoparticles, via a simple two-step fabrication process. Spray deposition and UV curing photopolymerization were sequentially performed, during which solvent evaporation provides microscale roughness while nanoparticle aggregation forms nanoscale roughness. The hierarchical morphologies were stabilized after UV curing photopolymerization. High contact angle (>150°) and low roll-off angle (<5°) were achieved due to the multiscale roughness structure of the hierarchical morphologies. These coatings also have excellent chemical resistance, as well as temperature and pH stability, after curing.
Combining Select Neuropsychological Assessment with Blood-Based Biomarkers to Detect Mild Alzheimer's Disease: A Molecular Neuropsychology Approach (2014)

The combination of demographics, two biomarkers, and one cognitive test created a biomarker-cognitive profile that was highly accurate in detecting the presence of AD, even in the very early stages.
Money, Money, Money (1994)

Research Article| October 01 1994 Money, Money, Money Randy Moore Randy Moore Search for other works by this author on: This Site PubMed Google Scholar The American Biology Teacher (1994) 56 (7): 388–389. https://doi.org/10.2307/4449866 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Randy Moore; Money, Money, Money. The American Biology Teacher 1 October 1994; 56 (7): 388–389. doi: https://doi.org/10.2307/4449866 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentThe American Biology Teacher Search This content is only available via PDF. Copyright 1994 National Association of Biology Teachers Article PDF first page preview Close Modal You do not currently have access to this content.
Synthesis and SAR of Thrombin Inhibitors Incorporating a Novel 4-Amino-Morpholinone Scaffold:  Analysis of X-ray Crystal Structure of Enzyme Inhibitor Complex (2003)

A 4-amino-2-carboxymethyl-3-morpholinone structural motif derived from malic acid has been used to mimic d-Phe-Pro in the thrombin inhibiting tripeptide d-Phe-Pro-Arg. The arginine in D-Phe-Pro-Arg was replaced by the more rigid P1 truncated p-amidinobenzylamine (Pab). These new thrombin inhibitors were used to probe the inhibitor binding site of alpha-thrombin. The best candidate in this series of thrombin inhibitors exhibits an in vitro IC50 of 0.130 microM. Interestingly, the stereochemistry of the 4-amino-2-carboxymethyl-3-morpholinone motif is reversed for the most active compounds compared to that of a previously reported 2-carboxymethyl-3-morpholinone series. The X-ray crystal structure of the lead inhibitor cocrystallized with alpha-thrombin is discussed.
Generation of Hepatitis C Virus-Like Particles by Use of a Recombinant Vesicular Stomatitis Virus Vector (2002)

Hepatitis C virus (HCV), a major etiologic agent of hepatocellular carcinoma, presently infects approximately 400 million people worldwide, making the development of protective measures against HCV infection a key objective. Here we have generated a recombinant vesicular stomatitis virus (VSV), which expresses the HCV structural proteins, by inserting the contiguous Core, E1, and E2 coding region of HCV into the VSV genome. Recombinant VSV expressing HCV Core, E1, and E2 (VSV-HCV-C/E1/E2) grew to high titers in vitro and efficiently expressed the incorporated HCV gene product, which became fully processed into the individual HCV structural proteins. Biochemical and biophysical analysis indicated that the HCV Core, E1, and E2 proteins assembled to form HCV-like particles (HCV-LPs) possessing properties similar to the ultrastructural properties of HCV virions. Mice immunized with VSV-HCV-C/E1/E2 generated cell-mediated immune responses to all of the HCV structural proteins, and humoral responses, particularly to E2, were also readily evident. Our data collectively indicate that engineered VSVs expressing HCV Core, E1, and E2 and/or HCV-LPs represent useful tools in vaccine and immunotherapeutic strategies designed to address HCV infection.
Topological arrangement of the major structural features of fibronectin. (1980)

Using tryptic cleavage coupled with biosynthetic labeling, we have established the order along the polypeptide chain of the major tryptic fragments derived from dimeric fibronectin.The dimer is held together by interchain disulfide(s) all of which are very close to the COOH-terminal.This COOH-terminal region is very readily removed by proteolysis.The NH2-terminal region of the molecule is extremely cystine-rich and is released in a 25,000-dalton fragment.The rest of the molecule remains as a 200,000-dalton internal fragment.By the use of cyanide cleavage, we have shown that the large fragment contains a disulfide-rich region at its NHz-terminal, adjacent to the 25-kd fragment, and contains a free sulfhydryl group which is 170,000 daltons from the NHz-terminal of the intact 230,000-dalton chain.There may be another free sulilydryl group 30,000 to 40,000 daltons further toward the COOH-terminal.The free sulfhydryl groups are necessary for binding of fibronectin to the cell surface and apparently participate in the formation of high molecular weight aggregates which contain fibronectin and are held together by disulfide bonds.These results, together with others in the literature, have allowed us to locate within the molecule the sites at which fibronectin binds to collagen and fibrin and the region at which these proteins are cross-linked to fibronectin by factor XIII transglutaminase.The crosslinking region is in the NHz-terminal 25-kd fragment and the binding site is immediately adjacent in the cystine-rich region of the 200-kd fragment.Fibronectin is a large glycoprotein present on the surfaces of many cell types (1-3).A related molecule is present in plasma (4).The major role of fibronectin is probably adhesion of cells to the substratum (5-7), and cell-surface fibronectin appears to be connected to actin microfilament bundles inside cells (8,9).These properties could explain some of the changes in behavior of malignant cells which have lost fibronectin on transformation (1-3).Fibronectin also appears to be involved in cell migration in vitro (10) and conceivably also in vivo (11, 12).Fibronectin binds strongly to gelatin and less strongly to
Development of multiplex real-time PCR for the rapid detection of five bacterial causes of community acquired pneumonia. (2011)

Establishing a microbial diagnosis for patients with community-acquired pneumonia (CAP) is still challenging and is often achieved in only 30-50% of cases. Polymerase chain reaction (PCR) has been shown to be more sensitive than conventional microbiological methods and it could help to increase the microbial yield for CAP patients. This study was designed to develop, optimize and evaluate multiplex real-time PCR as a method for rapid differential detection of five bacterial causes of CAP namely Streptococcus pneumoniae, Burkholderia pseudomallei and atypical bacterial pathogens, Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila. Duplex and triplex real-time PCR assays were developed using five sets of primers and probes that were designed based on an appropriate specific gene for each of the above CAP pathogens. The performance of primers for each organism was tested using SYBR Green melt curve analysis following monoplex realtime PCR amplification. Monoplex real-time PCR assays were also used to optimize each primers-probe set before combining them in multiplex assays. Two multiplex real-time PCR assays were then optimized; duplex assay for the differential detection of S. pneumoniae and B. pseudomallei, and triplex assay for the atypical bacterial pathogens. Both duplex and triplex real-time PCR assays were tested for specificity by using DNA extracted from 26 related microorganisms and sensitivity by running serial dilutions of positive control DNAs. The developed multiplex real-time PCR assays shall be used later for directly identifying CAP causative agents in clinical samples.
The Preparation and Proof of Structure of 1,2:5,6-Dicyclohexylidene-D-glucofuranose (1949)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Preparation and Proof of Structure of 1,2:5,6-Dicyclohexylidene-D-glucofuranoseRobert C. Hockett, Robert Ellsworth. Miller, and Allen. ScattergoodCite this: J. Am. Chem. Soc. 1949, 71, 9, 3072–3076Publication Date (Print):September 1, 1949Publication History Published online1 May 2002Published inissue 1 September 1949https://pubs.acs.org/doi/10.1021/ja01177a036https://doi.org/10.1021/ja01177a036research-articleACS PublicationsRequest reuse permissionsArticle Views157Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Functional and Structural Characterization of a Cation-dependent O-Methyltransferase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 (2008)

The coding sequence of the cyanobacterium Synechocystis sp. strain PCC 6803 slr0095 gene was cloned and functionally expressed in Escherichia coli. The corresponding enzyme was classified as a cation- and S-adenosyl-l-methionine-dependent O-methyltransferase (SynOMT), consistent with considerable amino acid sequence identities to eukaryotic O-methyltransferases (OMTs). The substrate specificity of SynOMT was similar with those of plant and mammalian CCoAOMT-like proteins accepting a variety of hydroxycinnamic acids and flavonoids as substrates. In contrast to the known mammalian and plant enzymes, which exclusively methylate the meta-hydroxyl position of aromatic di- and trihydroxy systems, Syn-OMT also methylates the para-position of hydroxycinnamic acids like 5-hydroxyferulic and 3,4,5-trihydroxycinnamic acid, resulting in the formation of novel compounds. The x-ray structure of SynOMT indicates that the active site allows for two alternative orientations of the hydroxylated substrates in comparison to the active sites of animal and plant enzymes, consistent with the observed preferred para-methylation and position promiscuity. Lys(3) close to the N terminus of the recombinant protein appears to play a key role in the activity of the enzyme. The possible implications of these results with respect to modifications of precursors of polymers like lignin are discussed.
Analysis of polymerase chain reaction amplifications through phosphate detection using an enzyme–based microbiosensor in a microfluidic device (2006)

An electrochemical method was developed for analyzing PCR amplification through the detection of inorganic phosphates (Pi). This method coupled a microchip to a nanoparticle comprising poly-5,2'-5',2''-terthiophene-3'-carboxylic acid (poly-TTCA)/pyruvate oxidase (PyO) modified microbiosensor. It detects Pi produced from the pyrophosphate (PPi), which is released as a byproduct of PCR. After completion of PCR, PPi is hydrolyzed to Pi by inorganic pyrophosphatase. On the microbiosensor surface, pyruvate was converted to H2O2 by PyO in the presence of Pi and oxygen, and subsequently, the anodic current of enzymatically generated H2O2 was detected at +0.5 V versus Ag/AgCl. The CE-EC analysis was completed within 2 min in a coated channel with 75.0 mm separation length at the field strength of -200 V/cm. Excellent operation stability of poly-TTCA/PyO was observed for a long period of analysis. The reproducibility of the analysis yielded an RSD of 3.4% (n = 22) for the peak areas and 1.8% (n = 22) for the migration times. The sensitivity of the analysis was 0.59 +/- 0.01 nA/cycle with a regression coefficient of 0.971.
Design and Experimental Verification of a 0.19 V 53 μW 65 nm CMOS Integrated Supply-Sensing Sensor With a Supply-Insensitive Temperature Sensor and an Inductive-Coupling Transmitter for a Self-Powered Bio-sensing System Using a Biofuel Cell (2017)

In this paper, we present a self-powered bio-sensing system with the capability of proximity inductive-coupling communication for supply sensing and temperature monitoring. The proposed bio-sensing system includes a biofuel cell as a power source and a sensing frontend that is associated with the CMOS integrated supply-sensing sensor. The sensor consists of a digital-based gate leakage timer, a supply-insensitive time-domain temperature sensor, and a current-driven inductive-coupling transmitter and achieves low-voltage operation. The timer converts the output voltage from a biofuel cell to frequency. The temperature sensor provides a pulse width modulation (PWM) output that is not dependent on the supply voltage, and the associated inductive-coupling transmitter enables proximity communication. A test chip was fabricated in 65 nm CMOS technology and consumed 53 μW with a supply voltage of 190 mV. The low-voltage-friendly design satisfied the performance targets of each integrated sensor without any trimming. The chips allowed us to successfully demonstrate proximity communication with an asynchronous receiver, and the measurement results show the potential for self-powered operation using biofuel cells. The analysis and experimental verification of the system confirmed their robustness.
Crystal Structure of a Superstable Mutant of Human p53 Core Domain (2004)

Most of the cancer-associated mutations in the tumor suppressor p53 map to its DNA-binding core domain. Many of them inactivate p53 by decreasing its thermodynamic stability. We have previously designed the superstable quadruple mutant M133L/V203A/N239Y/N268D containing the second-site suppressor mutations N239Y and N268D, which specifically restore activity and stability in several oncogenic mutants. Here we present the x-ray structure of this quadruple mutant at 1.9 A resolution, which was solved in a new crystal form in the absence of DNA. This structure reveals that the four point mutations cause only small local structural changes, whereas the overall structure of the central beta-sandwich and the DNA-binding surface is conserved. The suppressor mutation N268D results in an altered hydrogen bond pattern connecting strands S1 and S10, thus bridging the two sheets of the beta-sandwich scaffold in an energetically more favorable way. The second suppressor mutation N239Y, which is located in close proximity to the DNA-binding surface in loop L3, seems to reduce the plasticity of the structure in large parts of loop L3 as indicated by decreased crystallographic temperature factors. The same is observed for residues in the vicinity of the N268D substitution. This increase in rigidity provides the structural basis for the increase in thermostability and an understanding how N268D and N239Y rescue some of the common cancer mutants.
Human glandular salivas: their separate collection and analysis (1996)

Human saliva is secreted by the three pairs of major salivary glands (parotid, submandibular, and sublingual), and numerous minor ones, e.g. labial, buccal and (glosso)palatine glands. Using individually adapted collection devices, sublingual, submandibular, parotid and palatine secretions of five individuals were collected and analyzed. Electrophoretic analysis revealed that each type of saliva possesses characteristic features, despite interindividual variations. Parotid salivas are characterized by intensely staining amylase and proline-rich protein bands, but contain minute amounts of cystatins, lysozyme and the extra-parotid glycoprotein. Sublingual salivas are characterized by high concentrations of both types of salivary mucins, MG1 and MG2, and contain relatively high levels of lysozyme. Submandibular salivas contain highest concentration of salivary cystatin S. Palatine secretions contain high molecular weight mucins and a relatively high amylase concentration.
Mechanistic Study of the Synergistic Antibacterial Activity of Combined Silver Nanoparticles and Common Antibiotics (2016)

A combination of silver nanoparticles (AgNPs) and an antibiotic can synergistically inhibit bacterial growth, especially against the drug-resistant bacteria Salmonella typhimurium. However, the mechanism for the synergistic activity is not known. This study chooses four classes of antibiotics, β-lactam (ampicillin and penicillin), quinolone (enoxacin), aminoglycoside (kanamycin and neomycin), and polykeptide (tetracycline) to explore their synergistic mechanism when combined with AgNPs against the multidrug-resistant bacterium Salmonella typhimurium DT 104. Enoxacin, kanamycin, neomycin, and tetracycline show synergistic growth inhibition against the Salmonella bacteria when combined with AgNPs, while ampicillin and penicillin do not. UV-vis and Raman spectroscopy studies reveal that all these four synergistic antibiotics can form complexes with AgNPs, while ampicillin and penicillin do not. The presence of tetracycline enhances the binding of Ag to Salmonella by 21% and Ag(+) release by 26% in comparison to that without tetracycline, while the presence of penicillin does not enhance the binding of Ag or Ag(+) release. This means that AgNPs first form a complex with tetracycline. The tetracycline-AgNPs complex interacts more strongly with the Salmonella cells and causes more Ag(+) release, thus creating a temporal high concentration of Ag(+) near the bacteria cell wall that leads to growth inhibition of the bacteria. These findings agree with the recent findings that Ag(+) release from AgNPs is the agent causing toxicity.
ChemInform Abstract: Stereochemistry of the Thermal Isomerization of Bicyclo(3.2.0)hept‐2‐ene to Bicyclo(2.2.1)hept‐2‐ene. (1988)

Abstract The thermal isomerization of (Ia) to yield (II) and (III) occurs with predominant inversion as well as with substantial retention (21%).
Multilevel inverter based active power filter using space vector modulation (2012)

In this paper five-level multilevel inverter based shunt active power filter using space vector modulation (SVM) is presented. Application of multilevel inverter in active filters effectively reduces harmonics in high voltage system without use of bulky and high cost transformer. Reference signals are generated by using p-q theory from sensed supply voltages and load currents. Space Vector modulation scheme is implemented to generate switching signals. The switching sequences are generated with respect to the triangle number, such that there will be one switching per state which reduces the individual device switching frequency. The simulation and experimental results show MLI based APF using SVM eliminates harmonics effectively.
Quench heater simulation for protection of superconducting coils (1999)

Protection of large energy stored epoxy impregnated magnets requires careful design and analysis of protection heaters which are attached to the winding of a solenoid at a specified radial position (usually the winding outer surface) and at periodic circumferential locations. The heaters can be activated passively or actively depending on the desired protection scheme. The heaters are insulated from the winding by glass cloth and in some cases are sandwiched between the winding and a steel overband for structure support. An update report on the National High Magnetic Field Laboratory (NHMFL) efforts on quench heaters design, simulation and analysis is presented. All protection analysis discussed is based on internal dump.
Autoantibodies associated with atherosclerosis. (2000)

Atherosclerosis is a multifactorial pathological process. In recent years, the immune factors associated with its initiation and progression have been investigated intensively. Several autoantigens and their respective autoantibodies have been suggested as factors in atherogenesis. This manuscript provides a review of autoantibodies directed towards oxidized low-density lipoprotein (oxLDL), cardiolipin, beta2-glycoprotein-I and heat-shock protein 60/65, and their association with human and murine atherosclerosis.
Optical bit-pattern recognition by use of dynamic gratings in erbium-doped fiber (1997)

We present a novel optical bit-pattern-recognition technique that uses erbium-doped fiber at room temperature. Counterpropagating beams write a patterned gain-depletion grating in pumped erbium-doped fiber. This grating, recorded in the erbium gain medium, can be used for correlation with other optical bit patterns. We have demonstrated correlation of arbitrary return-to-zero bit patterns of as many as 8 bits at 1 Gbit/s . Theory suggests that scaling to higher bit rates is feasible.
Novel Mono-alkyl Magnesium Complexes Stabilized by a Bulky β-Diketiminate Ligand:  Structural Characterization of a Coordinatively Unsaturated Trigonal System (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTNovel Mono-alkyl Magnesium Complexes Stabilized by a Bulky β-Diketiminate Ligand: Structural Characterization of a Coordinatively Unsaturated Trigonal SystemVernon C. Gibson, John A. Segal, Andrew J. P. White, and David J. WilliamsView Author Information Department of Chemistry, Imperial College South Kensington, London, SW7 2AY, U.K. Cite this: J. Am. Chem. Soc. 2000, 122, 29, 7120–7121Publication Date (Web):July 11, 2000Publication History Received1 February 2000Published online11 July 2000Published inissue 26 July 2000https://pubs.acs.org/doi/10.1021/ja000359nhttps://doi.org/10.1021/ja000359nrapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1501Altmetric-Citations96LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Alkyls,Coordination compounds,Ligands,Magnesium,Molecular structure Get e-Alerts