gebhart/patrepeval huggingface dataset:
Isolation of a full-length cDNA clone encoding a N-terminally variant form of the human retinoid X receptor β (1992)

Journal Article Isolation of a full-length cDNA clone encoding a N-terminally variant form of the human retinoid X receptor β Get access K. Fleischhauer, K. Fleischhauer Search for other works by this author on: Oxford Academic PubMed Google Scholar J.H. Park, J.H. Park Search for other works by this author on: Oxford Academic PubMed Google Scholar J.P. DiSanto, J.P. DiSanto 1Laboratory of Human Immunogenetics, Memorial Sloan Kettering Cancer CenterNew York, NY 10021 Search for other works by this author on: Oxford Academic PubMed Google Scholar M. Marks, M. Marks 2Laboratory of Developmental and Molecular Immunity, National Institutes of HealthBethesda MD, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar K. Ozato, K. Ozato 2Laboratory of Developmental and Molecular Immunity, National Institutes of HealthBethesda MD, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar S.Y. Yang S.Y. Yang Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 20, Issue 7, 11 April 1992, Page 1801, https://doi.org/10.1093/nar/20.7.1801 Published: 11 April 1992 Article history Received: 06 March 1992 Published: 11 April 1992
A new test structure for the electrical measurement of the width of short features with arbitrarily wide voltage taps (1992)

Accurate determination of the linewidth of a narrow conducting film for VLSI applications using electrical test structure metrology has required that the length of the line be many times its width to minimize geometric error due to the finite width of the voltage taps. However, long lines obscure important local effects such as nonuniformities in the film. Shorter lines highlight such effects. This work describes a method of measuring the width of a short line having taps of arbitrary width. The effect of the taps is measured and used in the extraction of the linewidth allowing the determination of local linewidth variations with confidence.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Specification of individual Slouch muscle progenitors in <i>Drosophila</i> requires sequential Wingless signaling (2005)

The patterning of the Drosophila mesoderm requires Wingless (Wg), one of the founding members of a large family of secreted glycoproteins, the Wnt family. Little is known about how Wg provides patterning information to the mesoderm, which is neither an epithelium nor contains the site of Wg production. By studying specification of muscle founder cells as marked by the lineage-specific transcription factor Slouch, we asked how mesodermal cells interpret the steady flow of Wg. Through the manipulation of place, time and amount of Wg signaling, we have observed that Slouch founder cell cluster II is more sensitive to Wg levels than the other Slouch-positive founder cell clusters. To specify Slouch cluster I, Wg signaling is required to maintain high levels of the myogenic transcriptional regulator Twist. However, to specify cluster II, Wg not only maintains high Twist levels, but also provides a second contribution to activate Slouch expression. This dual requirement for Wg provides a paradigm for understanding how one signaling pathway can act over time to create a diverse array of patterning outcomes.
270-km 10 Gbit/s WDM dispersion compensation using a chirped AWGM (2003)

We show that a 10-Gbit/s per channel SM-fiber wavelength-division multiplexed (WDM) system could be extended from 70 km to 270 km using an array-waveguide multiplexer (AWGM) with a quadratic phase shift added across its waveguide array.
Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property (2008)

We document the protocols and methods for the production of immortalized cell lines of human neural stem cells from the human fetal central nervous system (CNS) cells by using a retroviral vector encoding v-myc oncogene. One of the human neural stem cell lines (HB1.F3) was found to express nestin and other specific markers for human neural stem cells, giving rise to three fundamental cell types of the CNS: neurons, astrocytes, and oligodendrocytes. After transplantation into the brain of mouse model of stroke, implanted human neural stem cells were observed to migrate extensively from the site of implantation into other anatomical sites and to differentiate into neurons and glial cells.
Antisense inhibition of apolipoprotein (a) to lower plasma lipoprotein (a) levels in humans (2016)

Epidemiological, genetic association, and Mendelian randomization studies have provided strong evidence that lipoprotein (a) [Lp(a)] is an independent causal risk factor for CVD, including myocardial infarction, stroke, peripheral arterial disease, and calcific aortic valve stenosis. Lp(a) levels >50 mg/dl are highly prevalent (20% of the general population) and are overrepresented in patients with CVD and aortic stenosis. These data support the notion that Lp(a) should be a target of therapy for CVD event reduction and to reduce progression of aortic stenosis. However, effective therapies to specifically reduce plasma Lp(a) levels are lacking. Recent animal and human studies have shown that Lp(a) can be specifically targeted with second generation antisense oligonucleotides (ASOs) that inhibit apo(a) mRNA translation. In apo(a) transgenic mice, an apo(a) ASO reduced plasma apo(a)/Lp(a) levels and their associated oxidized phospholipid (OxPL) levels by 86 and 93%, respectively. In cynomolgus monkeys, a second generation apo(a) ASO, ISIS-APO(a)Rx, significantly reduced hepatic apo(a) mRNA expression and plasma Lp(a) levels by >80%. Finally, in a phase I study in normal volunteers, ISIS-APO(a)Rx ASO reduced Lp(a) levels and their associated OxPL levels up to 89 and 93%, respectively, with minimal effects on other lipoproteins. ISIS-APO(a)Rx represents the first specific and potent drug in clinical development to lower Lp(a) levels and may be beneficial in reducing CVD events and progression of calcific aortic valve stenosis.
ChemInform Abstract: Electrochemical Intercalation of Lithium in V2O5 in Molten Dimethylsulfone at 130 °C. (1988)

Abstract A comparison of theoretical and experimental galvanostatic discharge/charge curves indicates that in molten dimethylsulfone Li behaves as an ideally dispersed solute in the V 2 O 5 host.
Separation of Ionic Compounds by Reversed-Phase Liquid Chromatography An Update of Ion-Pairing Techniques (1980)

Reversed-phase separation systems have become the most popular mode of liquid chromatography because of the participation of the mobile phase in the equilibrium distribution of the sample molecules. Recent investigations have demonstrated that the retention of charged molecules on bonded phase packings due to the presence of appropriate ionic agents in the mobile phase is an attractive alternative to the ion-exchange mode. This article examines the use of ionic mobile phase modifiers for the retention of ionic compounds by reversed-phase liquid chromatography. The role of ion suppression and ion pairing is discussed with respect to current hypotheses and to the practical application of the technique. An expanded review of recent applications is included.
A constraint architectural description approach to self-organising component-based software systems (2004)

We propose a constraint-based structural architectural description language for specifying and reasoning about self-organising software architectures and for guiding their evolution. We also introduce a notion of tactics added into the new language so as to mitigate the NP-complete problem of constraint satisfaction used for self-organising the specified architectures.
Membrane topology of the GltS Na<sup>+</sup>/glutamate permease of<i>Escherichia coli</i> (2007)

The GltS Na+/glutamate permease of Escherichia coli is the most extensively studied member of the ESS family of bacterial glutamate:Na+ symporters. This paper presents the membrane topology analysis of the GltS with translational alkaline phosphatase and beta-galactosidase gene fusions generated by TnphoA, nested deletions and targeted fusions. The topology model suggested by the translational fusion technique is compared with the MemGen model and discussed in detail.
ChemInform Abstract: THIENYL ANALOGS OF THE ALKALOIDS. PART 3. ANALOGS OF ECHINORINE AND ECHINOPSINE; A CONVENIENT SYNTHESIS OF THIENO(3,2‐B)PYRIDINES (1979)

Abstract Der Aminothiophenester (I) reagiert mit dem Acetylendicarboxylat (II) zu dem Michael‐Addukt (III), dessen Cyclisierungsprodukt, das Thienopyridinon (IV), zu (V) nitriert bzw. zu (VIa) chloriert wird; (VIa) wird zu dem Ether (VIb) bzw. zu dem Anilin (VIc) umgesetzt.
Threshold Voltage Control in PMOSFETs with Polysilicon or Fully-Silicided Gates on Hf-Based Gate Dielectric Using Controlled Lateral Oxidation (2006)

Large threshold-voltage (Vt) shifts are seen in PMOSFETs with Hf-based highK gate dielectric and polysilicon gates. The polysilicon-highK interface can be modified by introducing a thin interfacial oxide layer using a controlled lateral oxidation (CLO) step after gate patterning. This can reduce the threshold voltage shift to within 0.1V of SiO2 reference for devices with gate length <1mm. The benefit of lower Vt with the lateral oxidation comes at the expense of increased EOT. We therefore tried CLO using fully-silicided NiSi gates since the poly-depletion gain can offset the EOT increase. Our results show that the Vt difference between SiO2 and HfSiO is within 50mV. Moreover, the Ion-Ioff performance shows that while poly gates are lower in performance due to high Vt, the FUSI gates give Ion/Ioff at 1.1V of 180 (μA/μm)/20 (pA/μm) for both Hf- silicates and conventional SiO2. Interfacial modification using CLO can be a potential integration solution for highK gate dielectrics with polysilicon gates.
High-Efficiency LED Driver Without Electrolytic Capacitor for Street Lighting (2012)

High-brightness light-emitting diodes (LEDs) are considered as a remarkable lighting device due to their high reliability, chromatic variety, and increasing efficiency. As a consequence, a high number of solutions for supplying LED strings are coming out. One-stage solutions are cost effective, but their efficiency is low as they have to fulfill several purposes with only one converter: power factor correction (PFC), galvanic isolation (in some cases), and current regulation. Two-stage and three-stage solutions have higher efficiency as each stage is optimized for just one or two tasks, and they are the preferred option when supplying several strings at the same time. Nevertheless, due to their higher cost in comparison to one-stage solutions, they are used when high efficiency, high performance, and the possibility of supplying several strings are the main concerns. In addition, they are also used when high reliability is needed and electrolytic capacitors cannot be used. In this paper, a three-stage solution and its complete design guideline for LED-based applications is proposed. PFC is achieved by a boost converter, while the galvanic isolation is provided by an electronic transformer (second stage). The third stages (one for each LED string) are designed following the two-input buck schematic, but taking advantage of the load characteristics (i.e., the high value of the LED string knee voltage, approximately equal to half the string nominal voltage). Moreover, a variation of the analog driving technique is also proposed. Experimental results obtained with a 160-W prototype show an efficiency as high as 93% for the whole topology and 95% for the cascade connection of the second and third stages.
Demokratische Wohlfahrtsstaaten: neuere Befunde des internationalen Vergleichs in neun Thesen (2001)

"Mit einem Überblick über die Ergebnisse vergleichender Forschung in der Abteilung 'Theorie und Verfassung des Wohlfahrtstaates' stellt der Autor in neun Thesen neuere Befunde der Forschung zu den Bestimmungsfaktoren und dem Leistungsprofil demokratischer Wohlfahrtsstaaten vor. Die Thesen arbeiten heraus, dass demokratische Staatsverfassungen grundsätzlich besonders günstige Rahmenbedingungen für den Auf- und Ausbau einer ehrgeizigen Sozialpolitik enthalten, wenngleich die sozialpolitischen Aktivitäten stark variieren. Untersucht werden die Bestimmungsfaktoren für das Wachstum des Sozialstaats und deren Bedeutung für Bestrebungen zum Um- oder Rückbau von Sozialpolitik, auch hier zeigen sich größere Unterschiede zwischen den untersuchten Ländern. In der Bundesrepublik Deutschland häufen sich eher zahllose kleine, schrittweise erfolgende Kursänderungen, die insgesamt, so der Autor, doch zu einer beachtlichen Lern– und Fehlerkorrekturfähigkeit geführt haben. Der Autor fragt, ob der Reformspielraum in der Bundesrepublik Deutschland groß genug ist, um die wichtigsten Herausforderungen der Sozialpolitik abzuarbeiten und kommt schließlich zu der Einschätzung, dass in Deutschland ein deutliches Missverhältnis zwischen gut ausgebauter Sozialpolitik einerseits und der Vernachlässigung von anderen Politikfeldern, die für die Zukunftssicherung ebenso wichtig sind, bestehe. Schmidt formuliert 'begründete Hoffnung' für die Reformfähigkeit der Bundesrepublik Deutschland, die, wenn auch im einzelnen strittig, bislang doch zu größeren Korrekturen fähig war als andere Regime, beispielsweise die der ehemals sozialistischen Wohlfahrtsstaaten. Abschließend verweist der Autor auf die doppelte Wirkung der Sozialpolitik, zugleich Problemlöser und Problemerzeuger, Nutzenstifter und Kostenverursacher zu sein." (Autorenreferat)
DNA hypomethylation is prevalent even in low-grade breast cancers (2004)

Hypomethylation of some portions of the genome and hypermethylation of others are very frequent attributes of human cancer. We previously showed that cancer-associated DNA hypomethylation often involves satellite 2 (Sat2), the main DNA component of the large juxtacentromeric (centromere-adjacent) heterochromatin of chromosome 1. In this study, we compared methylation of Sat2 and centromeric satellite DNA (Satalpha) as well as overall genomic methylation in 41 breast adenocarcinomas of known tumor grade and stage, 16 non-neoplastic breast tissues (mostly fibroadenomas), and a variety of normal somatic tissue controls. The cancers were significantly hypomethylated at Sat2 relative to the fibroadenomas or normal somatic tissues and at Satalpha relative to the normal somatic tissues. However, unlike Sat2, Satalpha did not display significant differences in methylation between the cancers and the non-neoplastic breast tissues. Therefore, hypomethylation at Sat2 is a much better marker of breast cancer than is Satalpha hypomethylation. There was a significant association of Sat2 hypomethylation with global DNA hypomethylation in the cancers but not with tumor grade, stage, axillary lymph node involvement, or hormone receptor status. Extensive cancer-associated hypomethylation of juxtacentromeric satellite DNA and global DNA hypomethylation were common even in grade-1 or stage-1 carcinomas, which suggests that demethylation of the genome is an early event in breast carcinogenesis.
Region based selective image encryption (2006)

Image security has found a great need in many applications where the information (in the form of image) is to be protected from unauthorized access. Encrypting the image makes this possible. Several schemes for image encryption have been proposed. These schemes produce randomness in the image so that the content is not visible. Encryption and decryption consume a considerable amount of time. So there is a need for an efficient algorithm. In this paper, a region based selective image encryption technique is proposed which provides the facilities of selective encryption and selective reconstruction of images. Simulation results are presented and a comparative analysis of the proposed technique with the conventional methods is discussed. Also, the efficiency considerations and advantages of the new technique over the conventional methods are highlighted.
CubeSat Fabrication through Additive Manufacturing and Micro-Dispensing (2011)

Fabricating entire systems with both electrical and mechanical content through on-demand 3D printing is the future for high value manufacturing. In this new paradigm, conformal and complex shapes with a diversity of materials in spatial gradients can be built layer-by-layer using hybrid Additive Manufacturing (AM). A design can be conceived in Computer Aided Design (CAD) and printed on-demand. This new integrated approach enables the fabrication of sophisticated electronics in mechanical structures by avoiding the restrictions of traditional fabrication techniques, which result in stiff, two dimensional printed circuit boards (PCB) fabricated using many disparate and wasteful processes. The integration of Additive Manufacturing (AM) combined with Direct Print (DP) micro-dispensing and robotic pick-and-place for component placement can 1) provide the capability to print-on-demand fabrication, 2) enable the use of micron-resolution cavities for press fitting electronic components and 3) integrate conductive traces for electrical interconnect between components. The fabrication freedom introduced by AM techniques such as stereolithography (SL), ultrasonic consolidation (UC), and fused deposition modeling (FDM) have only recently been explored in the context of electronics integration and 3D packaging. This paper describes a process that provides a novel approach for the fabrication of stiff conformal structures with integrated electronics and describes a prototype demonstration: a volumetrically-efficient sensor and microcontroller subsystem scheduled to launch in a CubeSat designed with the CubeFlow methodology.
Shallow Quantum Circuits with Uninitialized Ancillary Qubits. (2016)

We study the computational power of shallow quantum circuits with $n$ input qubits, one output qubit, and two types of ancillary qubits: $O(\log n)$ initialized and $O({\rm poly}(n))$ uninitialized qubits. The initial state of the uninitialized ancillary qubits is arbitrary, and we have to return their state into the initial one at the end of the computation. First, to show the strengths of such circuits, we consider a class of symmetric functions on $n$ bits, including those (such as threshold functions) for which it is not known whether there exist shallow quantum circuits with only $O(\log n)$ initialized ancillary qubits. We show that there exists an $O(\log^2 n)$-depth quantum circuit for any function in the class with $O(\log n)$ initialized and $O(n\log^2 n)$ uninitialized ancillary qubits. Its existence shows the possibility that augmenting uninitialized ancillary qubits increases the computational power of shallow quantum circuits. The depth decreases to $O(\log n)$ when we use unbounded fan-out gates and unbounded Toffoli gates. Then, we consider the limitations of shallow quantum circuits with uninitialized ancillary qubits, which include unbounded fan-out gates and unbounded Toffoli gates. We show that, when the number of qubits on which unbounded fan-out gates act is $O({\rm poly}(\log n))$, for any constant $0 \leq \delta <1$, there does not exist an $O(\log^{\delta} n)$-depth quantum circuit for the parity function on $n$ bits with $O(\log n)$ initialized and $O({\rm poly}(n))$ uninitialized ancillary qubits.
Molecular and Structural Characterization of Polydisperse Polymers and Copolymers by Combining MALDI-TOF Mass Spectrometry with GPC Fractionation (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular and Structural Characterization of Polydisperse Polymers and Copolymers by Combining MALDI-TOF Mass Spectrometry with GPC FractionationGiorgio Montaudo, Domenico Garozzo, Maurizio S. Montaudo, Concetto Puglisi, and Filippo SamperiCite this: Macromolecules 1995, 28, 24, 7983–7989Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/ma00128a003https://doi.org/10.1021/ma00128a003research-articleACS PublicationsRequest reuse permissionsArticle Views2038Altmetric-Citations161LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Genetic basis of the RhD-positive and RhD-negative blood group polymorphism as determined by Southern analysis (1991)

Several lines of evidence have previously indicated that the RhD, c, and E blood group antigens are most likely carried by three distinct but homologous red blood cell membrane proteins. To determine whether these polypeptides are encoded by one or several related genes, we have performed Southern blot analysis of genomic DNA prepared from donors of different Rh phenotypes. Using an entire Rh cDNA probe and several exon-specific probes covering the cloned gene from its 5' to 3' ends, we have shown that the Rh locus carried by the genome of RhD-positive individuals is composed of two different but strongly related genes of identical general organization whether they expressed the C or c and E or e antigens, and, surprisingly, even when they do not express these epitopes, as in the D-- phenotype. The only antigenic variation found to be associated with a consistent genomic polymorphism corresponded to the RhD-positive/RhD-negative phenotypes. Indeed, one of the two Rh genes was completely lacking when the genomes of several unrelated RhD-negative donors were analyzed. From the present study we conclude that one of the two genes of the Rh locus encodes the RhC/c and RhE/e polypeptides while the other encodes the RhD protein. The absence of any D gene and of its postulated allelic form d in the RhD-negative genome explains finally why no Rhd antigen has ever been shown.
Complete transposition requires four active monomers in the mu transposase tetramer. (1994)

A tetramer of Mu transposase (MuA) cleaves and joins multiple DNA strands to promote transposition. Derivatives of MuA altered at two acidic residues that are conserved among transposases and retroviral integrases form tetramers but are defective in both cleavage and joining. These mutant proteins were used to analyze the contribution of individual monomers to the activity of the tetramer. The performance of different protein combinations demonstrates that not all monomers need to be catalytically competent for the complex to promote an individual cleavage or joining reaction. Furthermore, the results indicate that each pair of essential residues is probably donated to the active complex by a single monomer. Although stable, tetramers composed of a mixture of mutant and wild-type MuA generate products cleaved at only one end and with only one end joined to the target DNA. The abundance of these abortive products and the ratios of the two proteins in complexes stalled at different steps indicate that the complete reaction requires the activity of all four monomers. Thus, each subunit of MuA appears to use the conserved acidic amino acids to promote one DNA cleavage or one DNA joining reaction.