gebhart/patrepeval huggingface dataset:
Binding of the recombinant proteinase inhibitor eglin <i>c</i> from leech <i>Hirudo medicinalis</i> to serine (Pro)enzymes: A comparative thermodynamic study (1991)

The binding of the recombinant proteinase inhibitor eglin c from the leech Hirudo medicinalis to serine (pro)enzymes belonging to the chymotrypsin and subtilisin families has been investigated from the thermodynamic viewpoint, between pH 4.5 and 9.5 and from 10 degrees C to 40 degrees C. The affinity of eglin c for the serine (pro)enzymes considered shows the following trend: Leu-proteinase [the leucine specific serine proteinase from spinach (Spinacia oleracea L.) leaves] greater than human leucocyte elastase congruent to human cathepsin G congruent to subtilisin Carlsberg congruent to bovine alpha-chymotrypsin greater than bovine alpha-chymotrypsinogen A congruent to porcine pancreatic elastase congruent to bovine beta-trypsin. The serine (pro)enzyme-inhibitor complex formation is an entropy-driven process. On increasing the pH from 4.5 to 9.5, the affinity of eglin c for the serine (pro)enzymes considered increases thus reflecting the acid pK shift of the invariant hystidyl catalytic residue from approximately to 6.9 in the free serine proteinases and bovine alpha-chymotrypsinogen A to congruent to 5.1 in the serine (pro)enzyme-inhibitor complexes. Considering the known molecular models, the observed binding behaviour of eglin c was related to the inferred stereochemistry of the serine (pro)enzyme-inhibitor contact regions.
Additions and Corrections-3-Isothiazolone-cis-3-Thiocyanoacrylamide Equilibria (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAdditions and Corrections-3-Isothiazolone-cis-3-Thiocyanoacrylamide EquilibriaW Crow and Nelson LeonardCite this: J. Org. Chem. 1965, 30, 12, 4396Publication Date (Print):December 1, 1965Publication History Published online30 July 2002Published inissue 1 December 1965https://pubs.acs.org/doi/10.1021/jo01023a632https://doi.org/10.1021/jo01023a632research-articleACS PublicationsRequest reuse permissionsArticle Views18Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The genome of Prunus mume (2012)

Prunus mume (mei), which was domesticated in China more than 3,000 years ago as ornamental plant and fruit, is one of the first genomes among Prunus subfamilies of Rosaceae been sequenced. Here, we assemble a 280M genome by combining 101-fold next-generation sequencing and optical mapping data. We further anchor 83.9% of scaffolds to eight chromosomes with genetic map constructed by restriction-site-associated DNA sequencing. Combining P. mume genome with available data, we succeed in reconstructing nine ancestral chromosomes of Rosaceae family, as well as depicting chromosome fusion, fission and duplication history in three major subfamilies. We sequence the transcriptome of various tissues and perform genome-wide analysis to reveal the characteristics of P. mume, including its regulation of early blooming in endodormancy, immune response against bacterial infection and biosynthesis of flower scent. The P. mume genome sequence adds to our understanding of Rosaceae evolution and provides important data for improvement of fruit trees.
A Web tool for asynchronous, collaborative development of electronic meeting agendas (2002)

World Wide Web (Web) tools for management of documents during formal, synchronous electronic meetings are lacking greatly at present. Such a tool provides tremendous potential for applying Web mechanisms, such as dynamic page creation, and user interfaces to document processes in meetings. Such a tool in this research is referred to as a Web Electronic Meeting Document Manager (WEMDM). The paper presents a WEMDM, Logan, discussing its meeting agenda support function. Logan provides a Web based automated process for asynchronous, collaborative development of a textual meeting agenda. The process includes high meeting participant involvement through participant contributions to the agenda. Construction of the final agenda is automated via secretarius moderation. Moderation supports filtration of contributions through a contribution rejection mechanism. Participants view rejected contributions, assisting them in contribution formulation.
Laser-assisted metalorganic molecular beam epitaxy of GaAs (1988)

We report preliminary studies of the growth of homoepitaxial GaAs by laser-assisted metalorganic molecular beam epitaxy, using triethylgallium (TEGa) and As4 sources and a 193 nm ArF excimer laser. Laser irradiation results in a high, selective-area growth rate at temperatures below 450 °C, where pyrolytic growth is very slow. The process is extremely efficient, with roughly unit probability for impinging TEGa molecules sticking and being dissociated by laser radiation to form GaAs. From the strong dependence on laser fluence, the growth enhancement process appears to be pyrolytic in nature (because of transient heating by the pulsed laser) and not photolytic. The cross section for photolysis must be at least ten times lower than the gas-phase value (9×10−18 cm2). The surface morphology of films grown at 400 °C is rough at threshold fluences (∼0.10 J/cm2), but becomes smooth at higher fluences (∼0.13 J/cm2). These regions with relatively smooth surfaces exhibit enhanced photoluminescence yields compared to areas receiving less intense laser radiation.
Combining ECN and RTT for Datacenter Transport (2017)

Datacenter transports should provide low average and tail flow completion times (FCT) to achieve desired application performance. While most prior datacenter transports take either ECN or RTT as congestion signal, this paper makes a case that both signals are indispensable: ECN, as a per-hop signal, is more effective to prevent packet loss; while RTT, as an end-to-end signal, controls end-to-end queueing delay better. As persistent low flow completion times imply low queueing delay and near zero packet loss, we introduce EAR, a new datacenter transport that hears and reacts to both ECN and RTT. Our preliminary results show that: 1) compared to delay-based DCTCP, EAR achieves up to 91% lower packet losses and 93% fewer timeouts; 2) compared to ECN-based DCTCP, EAR reduces RTT by up to 32% for cross-rack traffic in a 4-level fattree. As a result, EAR delivers persistent low average and tail completion times under various scenarios in large scale simulations.
Prevention of Hypotension during Spinal Anesthesia for Cesarean Delivery (2005)

Combination of a high-dose phenylephrine infusion and rapid crystalloid cohydration is the first technique to be described that is effective for preventing hypotension during spinal anesthesia for cesarean delivery.
Evaluation of Rate Adaptation Algorithms in IEEE 802.11 Networks (2020)

Wireless technologies are being used in various applications for their ease of deployment and inherent capabilities to support mobility. Most wireless standards supports multiple data rates that may vary between few Mbps to few Gbps. Reaching the maximum supported data rate is what most application seek for. Nevertheless, the choice of data rates is very closely related to the quality of communication links and their stability. IEEE 802.11 standard introduced multi-rate support, since then, a lot of research has been done on rate adaptation, dealing with the different parameters that lead to an estimation of the channel conditions and the metrics that affect the network performance. In this paper, we present some of the popular rate adaptation schemes and summarize their characteristics. We categorize them as well into different categories according to their design and functionalities in terms of the strategies that are used to estimate channel conditions and decision making. We implemented some algorithms from the different categories in the network simulator NS-3 in order to evaluate their performance under different scenarios in Ad hoc and infrastructure modes. We present the lessons learned as well as our insights for future research work that can enhance the current approaches in the literature.
Impact of Tenofovir dose Adjustment on Both Estimated Glomerular Filtration Rate and Tenofovir trough Concentration (2016)

TDF dose adjustment combined with therapeutic drug monitoring may be useful especially in patients at risk of kidney dysfunction.
A Method for the <sup>32</sup>P Labeling of Peptides or Peptide Nucleic Acid Oligomers (1998)

A novel approach to the radioactive labeling of peptides and PNA oligomers is described. It is based on the conjugation of a deoxynucleoside 3'-phosphate with the terminal amine of the substrate, followed by phosphorylation of the 5'-hydroxyl group of the nucleotide using T4 polynucleotide kinase and [gamma-32P]ATP.
18F-FDG PET/CT Findings in Cytomegalovirus Colitis (2018)

We present a case demonstrating the diagnostic work-up of a patient undergoing azathioprine treatment for inflammatory bowel disease (IBD), diagnosed with an acute cytomegalovirus (CMV) infection and CMV colitis. An <sup>18</sup>F-FDG positron emission tomography/computed tomography (PET/CT) performed 2 weeks after debut of symptoms revealed pathological <sup>18</sup>F-FDG uptake in the left side of the colon mucosa, mimicked activity of IBD. However, a diagnosis of CMV colitis was based on the presence of CMV IgM antibodies, a seroconversion of CMV IgG antibodies, presence of CMV DNA in plasma and the finding af CMV DNA in biopsies from the intestinal mucosa. The patient responded to treatment with ganciclovir. This case highlights that a positive <sup>18</sup>F-FDG PET/CT scan of the colon can be due to CMV colitis.
Acoustic Signals in the Homoptera: Behavior, Taxonomy, and Evolution (1985)

The use of acoustic signals in communication, both within and between species, occurs widely in the Insecta (2, 35, 36). The loud airborne sounds of many groups of Orthoptera and of the large cicadas among the Homoptera are well known (1). In these groups, where communication may take place be­ tween individuals at distances very much greater than their own body length, tympanal acoustic receptors are well developed (50, 66). Some small insects are known to produce low intensity sounds. Many more possess structures that appear to be sound-producing mechanisms, but which have never been properly investigated (26, 35, 36). In recent years consider­ able advances have been made in our knowledge and understanding of the use of sound signals by small insects. For example, in species of Drosophila, and probably also in many other small winged insects, very close range, pulsed signals between individuals of the same species are produced by low frequency wing vibrations. These may only be detected by other individuals, when in very close proximity to the signaller, by the use of a displacement receptor-in Drosophila the lightly balanced antennal arista (7). In order to communicate over longer distances small insects, because of the purely physical limitations of their size, would have to use very high frequency, or ultrasonic, sounds (49, 50). However, high frequency sounds are not suitable for communication in structurally complicated habitats dominated by plants (50). An alternative that has received much attention recently is the use
Hierarchical task-driven feature learning for tumor histology (2015)

Through learning small and large-scale image features, we can capture the local and architectural structure of tumor tissue from histology images. This is done by learning a hierarchy of dictionaries using sparse coding, where each level captures progressively larger scale and more abstract properties. By optimizing the dictionaries further using class labels, discriminating properties of classes that are not easily visually distinguishable to pathologists are captured. We explore this hierarchical and task-driven model in classifying malignant melanoma and the genetic subtype of breast tumors from histology images. We also show how interpreting our model through visualizations can provide insight to pathologists.
On building non-intrusive performance instrumentation blocks for CORBA-based distributed systems (2002)

A non-intrusive, reusable framework for collecting performance statistics of CORBA-based distributed systems is proposed. The usefulness of the framework in the context of comparing performance improvement techniques is described. Also discussed is application of this framework to a large telecommunications application and experimental results on its relative overhead.
Mechanism of the mutagenic action of hydroxylamine. IX. The UV-induced cleavage of the N-O bond in N<sup>4</sup>-hydroxy-and N<sup>4</sup>-methoxycytidine and N<sup>6</sup>-methoxyadenosine (1975)

The principal UV-induced (lambda = 2546nm) reaction of N4-hydroxy- and N4methoxycytidines and N6-methoxyadenosine in neutral aqueous solutions is cleavage of the exocyclic N-O bond with the respective formation of cytidine and adenosine. Quantum yields are 2.8x10(-3) and 2.2x10(-3) M/E for the first two compounds and 9.1x10(-3) M/E for N6-methoxyadenosine.
Theoretical vs Empirical Prediction of Aphakic Refraction (1987)

The aphakic refraction was predicted from preoperative and postoperative keratometry and axial length determinations in 43 patients subjected to cataract extraction without intraocular lens implantation using a theoretical and an empirical (Sanders, Retzlaff, Kraff [SRK]) method of calculation. The error (mean +/- SD; observed value minus expected value) of the theoretical vs the empirical method was 0.02 +/- 0.76 diopters (D) vs 0.66 +/- 0.72 D, respectively, when the preoperative data were used in the calculations, and 0.21 +/- 0.80 D vs 0.84 +/- 0.79 D, respectively, when the postoperative data were used in the calculations. The mean error was significantly different from zero (predicted values were lower than observed values) for the SRK method but not for the theoretical method. The mean error of the SRK method could be eliminated by adjusting the offset constant in the regression formula.
Substantial narrowing of the Niemann–Pick C candidate interval by yeast artificial chromosome complementation (1997)

Niemann-Pick disease type C (NP-C) is an autosomal recessive lipidosis linked to chromosome 18q11-12, characterized by lysosomal accumulation of unesterified cholesterol and delayed induction of cholesterol-mediated homeostatic responses. This cellular phenotype is identifiable cytologically by filipin staining and biochemically by measurement of low-density lipoprotein-derived cholesterol esterification. The mutant Chinese hamster ovary cell line (CT60), which displays the NP-C cellular phenotype, was used as the recipient for a complementation assay after somatic cell fusions with normal and NP-C murine cells suggested that this Chinese hamster ovary cell line carries an alteration(s) in the hamster homolog(s) of NP-C. To narrow rapidly the candidate interval for NP-C, three overlapping yeast artificial chromosomes (YACs) spanning the 1 centimorgan human NP-C interval were introduced stably into CT60 cells and analyzed for correction of the cellular phenotype. Only YAC 911D5 complemented the NP-C phenotype, as evidenced by cytological and biochemical analyses, whereas no complementation was obtained from the other two YACs within the interval or from a YAC derived from chromosome 7. Fluorescent in situ hybridization indicated that YAC 911D5 was integrated at a single site per CT60 genome. These data substantially narrow the NP-C critical interval and should greatly simplify the identification of the gene responsible in mouse and man. This is the first demonstration of YAC complementation as a valuable adjunct strategy for positional cloning of a human gene.
Identification and Thermodynamic Characterization of Molten Globule States of Periplasmic Binding Proteins (2007)

Molten globule-like intermediates have been shown to occur during protein folding and are thought to be involved in protein translocation and membrane insertion. However, the determinants of molten globule stability and the extent of specific packing in molten globules is currently unclear. Using far- and near-UV CD and intrinsic and ANS fluorescence, we show that four periplasmic binding proteins (LBP, LIVBP, MBP, and RBP) form molten globules at acidic pH values ranging from 3.0 to 3.4. Only two of these (LBP and LIVBP) have similar sequences, but all four proteins adopt similar three-dimensional structures. We found that each of the four molten globules binds to its corresponding ligand without conversion to the native state. Ligand binding affinity measured by isothermal titration calorimetry for the molten globule state of LIVBP was found to be comparable to that of the corresponding native state, whereas for LBP, MBP, and RBP, the molten globules bound ligand with approximately 5-30-fold lower affinity than the corresponding native states. All four molten globule states exhibited cooperative thermal unfolding assayed by DSC. Estimated values of DeltaCp of unfolding show that these molten globule states contain 28-67% of buried surface area relative to the native states. The data suggest that molten globules of these periplasmic binding proteins retain a considerable degree of long range order. The ability of these sequentially unrelated proteins to form highly ordered molten globules may be related to their large size as well as an intrinsic property of periplasmic binding protein folds.
Driving comfort, enjoyment and acceptance of automated driving – effects of drivers’ age and driving style familiarity (2018)

Automated driving has the potential to improve the safety and efficiency of future traffic and to extend elderly peoples’ driving life, provided it is perceived as comfortable and joyful and is accepted by drivers. Driving comfort could be enhanced by familiar automated driving styles based on drivers’ manual driving styles. In a two-stage driving simulator study, effects of driving automation and driving style familiarity on driving comfort, enjoyment and system acceptance were examined. Twenty younger and 20 older drivers performed a manual and four automated drives of different driving style familiarity. Acceptance, comfort and enjoyment were assessed after driving with standardised questionnaires, discomfort during driving via handset control. Automation increased both age groups’ comfort, but decreased younger drivers’ enjoyment. Younger drivers showed higher comfort, enjoyment and acceptance with familiar automated driving styles, whereas older drivers preferred unfamiliar, automated driving styles tending to be faster than their age-affected manual driving styles.Practitioner Summary: Automated driving needs to be comfortable and enjoyable to be accepted by drivers, which could be enhanced by driving style individualisation. This approach was evaluated in a two-stage driving simulator study for different age groups. Younger drivers preferred familiar driving styles, whereas older drivers preferred driving styles unaffected by age.
Efficacy and safety of<scp>PAC</scp>‐14028 cream – a novel, topical, nonsteroidal, selective<scp>TRPV</scp>1 antagonist in patients with mild‐to‐moderate atopic dermatitis: a phase<scp>II</scp>b randomized trial (2019)

PAC-14028 cream may be an effective and safe treatment modality for the treatment of patients with mild-to-moderate AD.
Analysis of microsatellite instability in medulloblastoma (2009)

Medulloblastoma is the most common malignant brain tumor in children. The presence of microsatellite instability (MSI) in brain tumors, particularly medulloblastomas, has not been properly addressed. The aim of the present study was to evaluate the role of MSI in medulloblastoma carcinogenesis. MSI status was determined in 36 patients using a pentaplex PCR of quasimonomorphic markers (NR27, NR21, NR24, BAT25, and BAT26). Methylation status of mismatch repair (MMR) genes was achieved by methylation-specific multiplex ligation-dependent probe amplification (MLPA). In addition, MutS homolog 6 (MSH6) expression was determined by immunohistochemistry. Mutations of 10 MSI target genes (TCF4, XRCC2, MBD4, MRE11, ATR, MSH3, TGFBR2, RAD50, MSH6, and BAX) were studied by pentaplex PCR followed by analysis with GeneScan 3.7 software. Mutation analysis of hotspot regions of beta-catenin (CTNNB1) and BRAF (v-raf murine sarcoma viral oncogene homolog B1) oncogenes was performed by PCR single-strand conformation polymorphism analysis followed by direct sequencing. Among the 36 tumors, we found four (11%) cases with instability, one with high MSI and three with low MSI. Methylation analysis of MMR genes in cases presenting shifts on the MSI markers revealed mild hypermethylation of MSH6 in 75% of cases, yet MSH6 was expressed in all the tumors. The MSI target genes MBD4 (methyl-CpG binding domain protein 4) and MRE11 (meiotic recombination 11 homolog A) were mutated in two different tumors. No CTNNB1 or BRAF mutations were found. This study is the most comprehensive analysis of MSI in medulloblastomas to date. We observed the presence of MSI together with mutations of MSI target genes in a small fraction of cases, suggesting a new genetic pathway for a role in medulloblastoma development.