gebhart/patrepeval huggingface dataset:
Combined Mutagenesis and Kinetics Characterization of the Bilin‐Binding GAF Domain of the Protein Slr1393 from the Cyanobacterium <i>Synechocystis</i> PCC6803 (2014)

The gene slr1393 from Synechocystis sp. PCC6803 encodes a protein composed of three GAF domains, a PAS domain, and a histidine kinase domain. GAF3 is the sole domain able to bind phycocyanobilin (PCB) as chromophore and to accomplish photochemistry: switching between a red-absorbing parental and a green-absorbing photoproduct state (λmax =649 and 536 nm, respectively). Conversions in both directions were followed by time-resolved absorption spectroscopy with the separately expressed GAF3 domain of Slr1393. Global fit analysis of the recorded absorbance changes yielded three lifetimes (3.2 μs, 390 μs, and 1.5 ms) for the red-to-green conversion, and 1.2 μs, 340 μs, and 1 ms for the green-to-red conversion. In addition to the wild-type (WT) protein, 24 mutated proteins were studied spectroscopically. The design of these site-directed mutations was based on sequence alignments with related proteins and by employing the crystal structure of AnPixJg2 (PDB ID: 3W2Z), a Slr1393 orthologous from Anabaena sp. PCC7120. The structure of AnPixJg2 was also used as template for model building, thus confirming the strong structural similarity between the proteins, and for identifying amino acids to target for mutagenesis. Only amino acids in close proximity to the chromophore were exchanged, as these were considered likely to have an impact on the spectral and dynamic properties. Three groups of mutants were found: some showed absorption features similar to the WT protein, a second group showed modified absorbance properties, and the third group had lost the ability to bind the chromophore. The most unexpected result was obtained for the exchange at residue 532 (N532Y). In vivo assembly yielded a red-absorbing, WT-like protein. Irradiation, however, not only converted it into the green-absorbing form, but also produced a 660 nm, further-red-shifted absorbance band. This photoproduct was fully reversible to the parental form upon green light irradiation.
Transition Metal Complexes with the Tetrathiosquarate Dianion as Bridging Bischelate Ligand (1979)

Organometallic compounds having a chain-type structure such as (1) are of interest as potential one-dimensional electrical conductors. An example of (1) is Ni25(C4S4)26K2·x H2O (x ≈ 8); the conductivity of the compressed powder at 20 °C is 5 × 10−4 Ω−1 cm−1. The monomeric complex (2) can be reduced irreversibly in two steps by voltammetry.
Accuracy of CT scan measurements of the medial temporal lobe in routine dementia diagnostics (2003)

The published threshold values regarding the CT scan MTL measurements for the diagnosis of AD may be invalid when applied by other radiology departments without a local standardisation and validation.
Neutralization of Cytomegalovirus Virions: The Role of Complement (1997)

Complement provides a key immunologic defense against invading pathogens; thus, a clear understanding of the interactions between cytomegalovirus (CMV) and complement may permit the development of strategies to enhance CMV neutralization. In the presence of specific anti-CMV antibodies, complement enhanced the neutralizing ability of serum by 2- to 3-fold. However, in the absence of specific anti-CMV antibodies, complement was ineffective in neutralizing CMV virions by plaque assay. Although complement alone did not mediate any neutralizing effect, CMV consumed complement activity from seronegative serum, resulting in the deposition of C3 on the virion. However, only in the presence of specific anti-CMV antibody did complement activation continue to the deposition of C9 on the virions. These results strongly suggest complement regulation by CMV virions that is modulated by anti-CMV antibody; this regulation may be attributed to three host complement regulators on the virions: CD55, CD46, and CD59.
In Situ Hybridization at the Electron Microscopic Level Using a Bromodeoxyuridine Labeled DNA Probe (1993)

A bromodeoxyuridine (BrdU) labeled DNA probe was used for in situ hybridization at the electron microscopic (EM) level. A BrdU labeled DNA probe was hybridized in situ to cryostat sections of paraformaldehyde fixed OCT compound embedded cultured HL-60 cells. After hybridization, some sections were incubated with FITC-conjugated anti-BrdU monoclonal antibody for fluorescence microscopy (FM), and others were embedded in Quetol for electron microscopy (EM). The ultrathin sections of Quetol-embedded specimens were incubated with the anti-BrdU monoclonal antibody and the immunoglobulin: gold colloid. In both FM and EM studies, the signals were concentrated in the rough endoplasmic reticulum. Moreover, some label was arranged from the nucleus to the cytoplasm at the EM level. Relatively simple methods using the BrdU labeled DNA probe for the detection of the defined nucleic acid sequence with reasonable tissue preservation and high resolution are described here. This method may be useful for developmental and disease related studies of specific mRNA in cells and tissues.
The second time around (2008)

A look at the new &quot;double jeopardy &quot; laws in Queensland So what is ’double jeopardy’ anyway? Most students taking a Legal Studies course will sooner or later come across the concept of ’double jeopardy’, an old p(mciple of English common law wttich emigrated to Australia along with the First Fleet, and ultimately became embedded in the criminal laws of all Australian states and territories. It means simply that no person may be placed on trial more than once in respect of any criminal act on their part, whether they are convicted or acquitted at the end of the first trial. This does not prevent any appeal being raised against the outcome of the first trial, because this is regarded as b~mg part of the first trial process. But once the appeal process has
Acquired Immunodeficiency With Reversed T4/T8 Ratios in Infants Born to Promiscuous and Drug-Addicted Mothers (1983)

A new syndrome of acquired immunodeficiency has been identified in seven children who were small for gestational age at birth and subsequently have exhibited failure to thrive, lymphadenopathy, parotitis, hepatosplenomegaly, interstitial pneumonia, and recurrent infections. All have a profound cell-mediated immunodeficiency with reversed T4/T8 ratios. Six are hypergammaglobulinemic and one has low IgG levels. The mothers of five of the seven children are sexually promiscuous and/or drug addicts. Three mothers have an immunodeficiency similar to that found in their infants. One of them died at age 33 years with a diagnosis of acquired immunodeficiency syndrome. In five of the children and in three of their mothers, there is evidence of a persistent Epstein-Barr virus (EBV) infection. We speculate that a perinatal or in utero transmission of EBV can induce an "infectious immunodeficiency." The clinical, histopathologic, and immunologic features resemble those described in adult homosexuals and drug addicts.
Human herpesvirus-7 (HHV-7). (1995)

HHV-7 first isolated in 1990 from a healthy individual, is a ubiquitous agent. The second independent isolation of HHV-7 from a chronic fatigue syndrome patient was reported in 1992. The seroepidemiology of HHV-7 suggested that its prevalence rate in the U.S.A. population is > 85%; however, in Japan a low prevalence rate has been reported. HHV-7 can be more readily isolated from the saliva than HHV-6. The primary infection of HHV-7 appears later in life than HHV-6. No disease has been reported that is etiologically linked to HHV-7. HHV-7 is more closely related to HHV-6 and the human cytomegalovirus than other members of the human herpesvirus family.
Proteomic and Genomic Characterization of Highly Infectious <i>Clostridium difficile</i> 630 Spores (2009)

Clostridium difficile, a major cause of antibiotic-associated diarrhea, produces highly resistant spores that contaminate hospital environments and facilitate efficient disease transmission. We purified C. difficile spores using a novel method and show that they exhibit significant resistance to harsh physical or chemical treatments and are also highly infectious, with <7 environmental spores per cm(2) reproducibly establishing a persistent infection in exposed mice. Mass spectrometric analysis identified approximately 336 spore-associated polypeptides, with a significant proportion linked to translation, sporulation/germination, and protein stabilization/degradation. In addition, proteins from several distinct metabolic pathways associated with energy production were identified. Comparison of the C. difficile spore proteome to those of other clostridial species defined 88 proteins as the clostridial spore "core" and 29 proteins as C. difficile spore specific, including proteins that could contribute to spore-host interactions. Thus, our results provide the first molecular definition of C. difficile spores, opening up new opportunities for the development of diagnostic and therapeutic approaches.
A dynamic scaling FFT processor for DVB-T applications (2004)

This paper presents an 8192-point FFT processor for DVB-T systems, in which a three-step radix-8 FFT algorithm, a new dynamic scaling approach, and a novel matrix prefetch buffer are exploited. About 64 K bit memory space can be saved in the 8 K point FFT by the proposed dynamic scaling approach. Moreover, with data scheduling and pre-fetched buffering, single-port memory can be adopted without degrading throughput rate. A test chip for 8 K mode DVB-T system has been designed and fabricated using 0.18-/spl mu/m single-poly six-metal CMOS process with core area of 4.84 mm/sup 2/. Power dissipation is about 25.2 mW at 20 MHz.
Nonthermal Plasma Processing for Air‐Pollution Control: A Historical Review, Current Issues, and Future Prospects (2004)

Abstract Summary: This review describes the historical development, current status, and future prospects for nonthermal plasma (NTP) technology and its application as an air‐pollution control. In particular, this review focuses on the cutting‐edge technology of hybrid NTP, where it is combined with other methods such as wet processing, the use of adsorbents, and catalysis. Historical landmarks in the development of NTP technology and the current status of large‐scale applications are discussed. The general characteristics of the combined system of NTP with catalysts are described in the context of the decomposition of NO x and benzene. Comparison of different NTP reactors for 200 ppm benzene decomposition (water vapor 0.5 vol.‐%). magnified image Comparison of different NTP reactors for 200 ppm benzene decomposition (water vapor 0.5 vol.‐%).
Portable system for monitoring of regional cerebral oxygen saturation during prehospital cardiopulmonary resuscitation: a pilot study (2014)

We developed an rSO <sub>2</sub> monitoring system for use during prehospital cardiopulmonary resuscitation. The monitoring system showed a significant increase in rSO <sub>2</sub> after return of spontaneous circulation, whereas there was no significant increase in rSO <sub>2</sub> during cardiopulmonary resuscitation after intubation but before return of spontaneous circulation.
16,20-Dimethylcholanthrene (1935)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT16,20-DimethylcholanthreneLouis F. Fieser and Arnold M. SeligmanCite this: J. Am. Chem. Soc. 1935, 57, 7, 1377–1378Publication Date (Print):July 1, 1935Publication History Published online1 May 2002Published inissue 1 July 1935https://pubs.acs.org/doi/10.1021/ja01310a059https://doi.org/10.1021/ja01310a059research-articleACS PublicationsRequest reuse permissionsArticle Views25Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Faraday cup detector for high‐mass ions in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (1995)

Abstract A detector has been developed, which collects and amplifies the electric charge of an incoming bunch of ions, for use in matrix‐assisted laser desorption/ionization (MALDI) time‐of‐flight (TOF) mass spectrometry. The detector has a time‐of‐flight resolution of about 50 ns and a noise level of 750 electrons rms. It has been tested in a Finnigan LaserMAT spectrometer with a number of samples which Were expected to give reliable results in MALDI time‐of‐flight mass spectrometry. Clear mass peaks have been observed from samples with masses above 300 kDa.
Specific transcriptional activation in vitro by the herpes simplex virus protein VP16 (1993)

The herpes simplex virus protein VP16 interacts with cellular factors, including the protein Oct-1, to activate viral immediate early (IE) gene transcription. We have reproduced this effect by addition of purified, full-length VP16 and the DNA-binding 'POU' domain of Oct-1 (Oct-1/POU) to a HeLa cell in vitro transcription system. Stimulation of transcription was dependent on the IE-specific element, TAATGARAT. In agreement with earlier observations from electrophoretic mobility shift assays, activation was not observed when Oct-2/POU, the DNA-binding domain from the Oct-2 protein, was substituted for Oct-1/POU. Single round transcription assays revealed that, together, VP16 and Oct-1/POU facilitate the assembly of pre-initiation complexes at target gene promoters.
Diagnosis, surveillance, and epidemiologic evaluation of viral infections in pediatric cardiac transplant recipients with the use of the polymerase chain reaction. (1996)

Polymerase chain reactions may be used as a rapid and sensitive method to evaluate postoperative viral infections in heart transplant recipients, especially in those with late-onset rejection or chronic rejection. Polymerase chain reaction may also be useful in the serial analysis of cytomegalovirus status in transplant recipients. The use of multiple viral primers improves the diagnostic evaluation of these patients and may lead to a better understanding of the epidemiologic characteristics of posttransplantation viral infections and the cause of late or chronic rejection.
Μέλος του Ομίλου Star Hotels (2010)

Κοσμοπολίτικη διάθeση και ποιότητα eίναι η eπιλογή στα standard δωμάτια του ξeνοδοχeίου. Υπάρχουν διαθέσιμα δωμάτια για μη καπνίζοντeς, eπικοινωνούντα δωμάτια μe eνδιάμeση πόρτα καθώς και δωμάτια προσβάσιμα από ανθρώπους μe κινητικά προβλήματα.
Expression of an ornithine decarboxylase dominant-negative mutant reverses eukaryotic initiation factor 4E-induced cell transformation. (1996)

pMV7-4E cells (4E-P2), which overexpress translation initiation factor eIF-4E, contain elevated levels of ornithine decarboxylase (ODC), the first and rate-limiting enzyme in polyamine biosynthesis. We have shown previously that this induction appears to be related to the transformed phenotype of these cells (L. M. Shantz and A. E. Pegg, Cancer Res., 54: 2313-2316, 1994). To test whether increased ODC activity is responsible for the transformation of 4E-P2 cells, a dominant-negative mutant of ODC was used to reduce the intracellular ODC activity in 4E-P2 cells, and the resulting phenotypic changes were examined. The mutant K69A/C360A contains mutations to alanine of two key active site residues, lysine 69 and cysteine 360, and is truncated at 425 amino acids. Combination of purified K69A/C360A and purified wild-type ODC resulted in a dose-dependent decrease in specific activity compared with wild-type ODC alone, with a 71% reduction at equimolar concentrations. This mutant was transfected into 4E-P2 cells, and stable clones that expressed the truncated K69A/ C360A were isolated. Several clones were tested for their ability to form transformed foci on a monolayer, grow in soft agar, and form tumors in nude mice. When ODC activity was reduced by 60%, the transformed phenotype of 4E-P2 cells was abolished, suggesting strongly that high ODC levels are critical to the transformation of these cells. In addition, K69A/C360A can be used to determine the ODC activity associated with transformation in both in vitro and in vivo systems.
Liveness Detection Using Implicit 3D Features (2018)

Spoofing attacks are a threat to modern face recognition systems. In this work we present a simple yet effective liveness detection approach to enhance 2D face recognition methods and make them robust against spoofing attacks. We show that the risk to spoofing attacks can be re- duced through the use of an additional source of light, for example a flash. From a pair of input images taken under different illumination, we define discriminative features that implicitly contain facial three-dimensional in- formation. Furthermore, we show that when multiple sources of light are considered, we are able to validate which one has been activated. This makes possible the design of a highly secure active-light authentication framework. Finally, further investigating the use of 3D features without 3D reconstruction, we introduce an approximated disparity-based implicit 3D feature obtained from an uncalibrated stereo-pair of cameras. Valida- tion experiments show that the proposed methods produce state-of-the-art results in challenging scenarios with nearly no feature extraction latency.
Coumarins and flavonoid from Murraya paniculata (L.) Jack: Antibacterial and anti-inflammation activity. (2015)

The ethyl acetate extract of leaves of Murraya paniculata (L.) Jack was described in the previous in vitro study on the inhibition effect on the growth of periodontopathic bacteria and the reduction of cytokines from LPS-stimulated macrophages. In this study, four coumarins including murrangatin (1), murrangatin acetate (2), murranganonesenecionate (3), micropubescin (4) and one flavonoid, 3', 4', 5', 7-tetramethoxyflavone (5) were isolated from the leaves of ethyl acetate extract of M. paniculata. MTT assay was used to test cytotoxicity on human gingival fibroblast and monocytes. The isolated compounds were evaluated for their antibacterial effect against Porphyromonas gingivalis (ATCC33277) and anti-inflammation on lipopolysaccharide-stimulated inflammation using monocyte cells. All isolated compounds exhibited antibacterial activity against P. gingivalis (ATCC 33277). Murranganonesenecionate (3) was highly potent anti-inflammation properties. The coumarin constituents from M. paniculata leaves might be potential lead molecules for the development of antimicrobial drugs for treating periodontal disease.
Cell surface proteases (2003)

Membrane Type - Matrix Metalloproteinases Surface Association of Secreted Matrix Metalloproteinases Biochemical Properties and Functions of Membrane-anchored Metalloprotease-Disintegrin Proteins (ADAMs) Shedding of plasma membrane proteins Expression of Meprins in Health and Disease Type II Transmembrane Serine Proteases DPPIV, Seprase and Related Serine Peptidases in Multiple Cellular Functions The Secretases of Alzheimer's Disease Plasminogen Activation at the Cell Surface Cell Surface Cathepsin B: Understanding its Functional Significance Protease activated receptors EMMPRIN (CD147), a cell surface regulator of Matrix Metalloproteinase Production and Function The evolving roles of Cell Surface Proteases in Health and Disease: Implications for Developmental, Adaptive, Inflammatory and Neoplastic Processes Shed Membrane Vesicles and Clustering of Membrane-bound Proteolytic Enzymes
Iron‐Catalyzed Carbonylation: Selective and Efficient Synthesis of Succinimides (2009)

A convenient one-pot method for the synthesis of various substituted succinimides has been developed. By starting from commercially available amines (or ammonia) and alkynes, a range of interesting succinimides have been obtained selectively in the presence of either [Fe(CO)5] or [Fe3(CO)12] (see scheme; R′=H, alkyl, aryl; R′′, R′′′=alkyl, aryl).
Allelic losses on chromosomes 14, 10, and 1 in atypical and malignant meningiomas: a genetic model of meningioma progression. (1995)

To investigate chromosomal events that underlie formation and progression of meningiomas, we have examined a set of 18 benign (WHO grade I), 15 atypical (grade II), and 13 anaplastic/malignant (grade III) meningiomas for loss of heterozygosity (LOH) on chromosomes 1p, 6p, 9q, 10q, and 14q. Frequent loss of loci on these chromosomes was seen in grade II and grade III tumors, specifically, 14q (II and III, 47 and 55%), 1p (40 and 70%), and 10q (27 and 40%). In contrast, LOH for these loci was infrequent in benign meningiomas, specifically, 14q (0%), 1p (11%), and 10q (12%). The smallest common regions of deletion that could be defined were 14q24-q32, 1p32-pter, and 10q24-qter. These observations indicate the likely presence of tumor suppressor genes in these regions that are involved in the development of WHO grade II and grade III meningiomas. Because LOH for loci on chromosomes 1p and 10q was found in tumors of all grades and because the frequency of LOH in all three regions increased with tumor grade, these results would support a model for the formation of aggressive meningiomas through tumor progression.
A comparative solubility enhancement profile of valdecoxib with different solubilization approaches (2007)

Solubility enhancement of poorly aqueous soluble drug is an important aspect of formulation development. Although there is plethora of reports of solubility improvement using different techniques, a comparative study of different solubilization approaches are few. Valdecoxib chemically designated as 4-(5-methyl-3-phenyl-4-isoxazolyl) benzenesulfonamide is a novel potent COX-2 inhibitor having poor aqueous solubility (10 mg/ml). Since it is poorly water-soluble, various techniques could be applied to increase its aqueous solubility. Objective of present study was to provide a comparison of effect of various solubilization techniques, namely micellar solubilization, cyclodextrin complexation and cosolvency, on solubility of valdecoxib. Solubility of valdecoxib was determined in various ionic and nonionic surfactants using phase solubility analysis. Similar type of study was performed using different water:cosolvent mixture. In addition, solubility improvement by use of 2 novel hydrophilic β -cyclodextrin derivatives, hydroxypropyl β -cyclodextrin and sulfobutyl ether-7-β -cyclodextrin was examined. Results showed that highest solubility (70 fold) was achieved with use of Cremophor EL followed by Tween 80 and sulfobutyl ether-7-β -cyclodextrin. It was found that surfactants with higher HLB values were better solubilizers. Solubilization capacity was found to increase with increase in hydrocarbon chain of surfactant, suggesting hydrocarbon core of micelles as locus of solubilization. Similarly, less polar solvents were found to increase solubility by greater extent, thus accentuating hydrophobic interaction mechanism. Among cyclodextrin, higher binding constant and solubility enhancement was obtained by use of sulfobutyl ether-7-β -cyclodextrin. Thus, the study generated an important dataset so as to compare effect of various solubilizers on solubility of valdecoxib.