gebhart/patrepeval huggingface dataset:
Impact acceleration injury in the rat: evidence for focal axolemmal change and related neurofilament sidearm alteration. (1997)

Recently we reported that traumatic brain injury evokes local changes in the axolemma's permeability, in concert with local cytoskeletal changes involving neurofilament (NF) compaction and sidearm loss, all of which contribute to the genesis of reactive axonal change. Since it was of concern that these events may be either injury model- or species-specific, we sought to address these phenomena in a different but well-characterized animal model and species. Further, to provide more compelling insight into the potential for NF compaction and sidearm alteration, we also employed antibodies specific for the NF rod domains, which are readily visualized only when the NF sidearms are disturbed. Rats were subjected to impact acceleration injury. To assess the potential for altered axolemmal permeability, 5 animals received intrathecal horseradish peroxidase (HRP), normally excluded by the intact axolemma. To assess the potential for NF sidearm alteration, another 14 animals were processed for the visualization of antibodies targeting the NF rod domain at 5 minutes (min) to 24 hours (h) postinjury. All animals were evaluated at the LM and EM levels. Those animals receiving intrathecal HRP showed immediate focal alterations in the axolemma's permeability to the normally excluded tracer. Over a 2 h period, these axons demonstrated NF compaction. Antibodies targeted to the rod domains revealed focal intra-axonal immunoreactivity in sites closely correlated with those showing altered axolemmal permeability. These same sites also demonstrated evidence of NF compaction and sidearm loss/perturbation. Collectively, these findings suggest that occurrence of altered axolemmal permeability and concomitant cytoskeletal change are features common to traumatic brain injury in various animal models and species. Further, these studies underscore the utility of antibodies targeting the rod domain for the early detection of traumatically induced reactive change.
Lipoid Pneumonia: A Silent Complication of Mineral Oil Aspiration (1999)

Lipoid pneumonia as a result of mineral oil aspiration still occurs in the pediatric population. It can mimic other diseases because of its nonspecific clinical presentation and radiographic signs. In patients with swallowing dysfunction and pneumonia, a history of mineral oil use should be obtained and a diagnosis of ELP should be considered in the differential diagnoses if mineral oil use has occurred. Our case points to the need for increased awareness by the general pediatricians of the potential hazards of mineral oil use for chronic constipation.
Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants (2002)

Plant HKT proteins comprise a family of cation transporters together with prokaryotic KtrB, TrkH, and KdpA transporter subunits and fungal Trk proteins. These transporters contain four loop domains in one polypeptide with a proposed distant homology to K(+) channel selectivity filters. Functional expression in yeast and Xenopus oocytes revealed that wheat HKT1 mediates Na(+)-coupled K(+) transport. Arabidopsis AtHKT1, however, transports only Na(+) in eukaryotic expression systems. To understand the molecular basis of this difference we constructed a series of AtHKT1/HKT1 chimeras and introduced point mutations to AtHKT1 and wheat HKT1 at positions predicted to be critical for K(+) selectivity. A single-point mutation, Ser-68 to glycine, was sufficient to restore K(+) permeability to AtHKT1. The reverse mutation in HKT1, Gly-91 to serine, abrogated K(+) permeability. This glycine in P-loop A of AtHKT1 and HKT1 can be modeled as the first glycine of the K(+) channel selectivity filter GYG motif. The importance of such filter glycines for K(+) selectivity was confirmed by interconversion of Ser-88 and Gly-88 in the rice paralogues OsHKT1 and OsHKT2. Surprisingly, all HKT homologues known from dicots have a serine at the filter position in P-loop A, suggesting that these proteins function mainly as Na(+) transporters in plants and that Na(+)/K(+) symport in HKT proteins is associated with a glycine in the filter residue. These data provide experimental evidence that the glycine residues in selectivity filters of HKT proteins are structurally related to those of K(+) channels.
Temperature Modulation of a Catalytic Gas Sensor (2014)

The use of catalytic gas sensors usually offers low selectivity, only based on their different sensitivities for various gases due to their different heats of reaction. Furthermore, the identification of the gas present is not possible, which leads to possible misinterpretation of the sensor signals. The use of micro-machined catalytic gas sensors offers great advantages regarding the response time, which allows advanced analysis of the sensor response. By using temperature modulation, additional information about the gas characteristics can be measured and drift effects caused by material shifting or environmental temperature changes can be avoided. In this work a miniaturized catalytic gas sensor which offers a very short response time (<150 ms) was developed. Operation with modulated temperature allows analysis of the signal spectrum with advanced information content, based on the Arrhenius approach. Therefore, a high-precise electronic device was developed, since theory shows that harmonics induced by the electronics must be avoided to generate a comprehensible signal.
Sensory Activity, Chemical Structure, and Synthesis of Maillard Generated Bitter-Tasting 1-Oxo-2,3-dihydro-1<i>H</i>-indolizinium-6-olates (2003)

Thermal treatment of aqueous solutions of xylose, rhamnose, and l-alanine led to a rapid development of a bitter taste of the reaction mixture. To characterize the key compounds causing this bitter taste, the recently developed taste dilution analysis (TDA), which is based on the determination of the taste threshold of reaction products in serial dilutions of HPLC fractions, was performed to locate the most intense taste compounds in the complex mixture of Maillard reaction products. By application of this TDA, 26 fractions were obtained, among which seven fractions were evaluated with a high taste impact. LC/MS and NMR spectroscopy as well as synthetic experiments revealed the 1-oxo-2,3-dihydro-1H-indolizinium-6-olates 1-5 as the key compounds contributing the most to the intense bitter taste of the Maillard mixture. Calculation of the taste impact of these compounds based on a dose/activity relationship indicated that these five compounds already accounted for 56.8% of the overall bitterness of the Maillard mixture, thus demonstrating this class of 1-oxo-2,3-dihydro-1H-indolizinium-6-olates as the key bitter compounds. First synthetic studies on the relationship between the chemical structure and the human psychobiological activity of 1-oxo-2,3-dihydro-1H-indolizinium-6-olates revealed that substitution of the furan rings of 1 by 5-methylfuryl moieties (compounds 3-5) or by 5-(hydroxymethyl)furyl groups (compound 6) led to a significant increase of the bitter threshold. In contrast, the substitution of the oxygen atoms in the furan rings of 1 by sulfur atoms induced a significant decrease of the detection threshold of the 1-oxo-2,3-dihydro-1H-indolizinium-6-olate; for example, the thiophene derivative 7 showed the extraordinarily low bitter detection threshold of 6.3 x 10(-5) mmol/kg (water).
Nitrosopersulfide (SSNO−) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide (2014)

Sulfide salts are known to promote the release of nitric oxide (NO) from S-nitrosothiols and potentiate their vasorelaxant activity, but much of the cross-talk between hydrogen sulfide and NO is believed to occur via functional interactions of cell regulatory elements such as phosphodiesterases. Using RFL-6 cells as an NO reporter system we sought to investigate whether sulfide can also modulate nitrosothiol-mediated soluble guanylyl cyclase (sGC) activation following direct chemical interaction. We find a U-shaped dose response relationship where low sulfide concentrations attenuate sGC stimulation by S-nitrosopenicillamine (SNAP) and cyclic GMP levels are restored at equimolar ratios. Similar results are observed when intracellular sulfide levels are raised by pre-incubation with the sulfide donor, GYY4137. The outcome of direct sulfide/nitrosothiol interactions also critically depends on molar reactant ratios and is accompanied by oxygen consumption. With sulfide in excess, a 'yellow compound' accumulates that is indistinguishable from the product of solid-phase transnitrosation of either hydrosulfide or hydrodisulfide and assigned to be nitrosopersulfide (perthionitrite, SSNO(-); λ max 412 nm in aqueous buffers, pH 7.4; 448 nm in DMF). Time-resolved chemiluminescence and UV-visible spectroscopy analyses suggest that its generation is preceded by formation of the short-lived NO-donor, thionitrite (SNO(-)). In contrast to the latter, SSNO(-) is rather stable at physiological pH and generates both NO and polysulfides on decomposition, resulting in sustained potentiation of SNAP-induced sGC stimulation. Thus, sulfide reacts with nitrosothiols to form multiple bioactive products; SSNO(-) rather than SNO(-) may account for some of the longer-lived effects of nitrosothiols and contribute to sulfide and NO signaling.
Polymeric phosphonitrilic esters: A new type of inorganic‐organic plastics (1948)

Abstract The attempt to replace chlorine in polyphosphonitrilic chloride with alkoxyl groups is successful except that oxygen rather than alkoxyl substitution occurs to some extent. The amount of oxygen substitution and the extent of degradation are minimized if the polyphosphonitrilic chloride is swollen prior to alcoholysis. The substituted product is insoluble until treated with boiling tetrachloroethane. The final products, the esters, are very tacky when pure but can be incorporated with other polymers to give desirable products.
Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified <i>Aedes aegypti</i> (2006)

Mosquitoes (Aedes aegypti) were genetically modified to exhibit impaired vector competence for dengue type 2 viruses (DENV-2). We exploited the natural antiviral RNA interference (RNAi) pathway in the mosquito midgut by constructing an effector gene that expresses an inverted-repeat (IR) RNA derived from the premembrane protein coding region of the DENV-2 RNA genome. The A. aegypti carboxypeptidase A promoter was used to express the IR RNA in midgut epithelial cells after ingestion of a bloodmeal. The promoter and effector gene were inserted into the genome of a white-eye Puerto Rico Rexville D (Higgs' white eye) strain by using the nonautonomous mariner MosI transformation system. A transgenic family, Carb77, expressed IR RNA in the midgut after a bloodmeal. Carb77 mosquitoes ingesting an artificial bloodmeal containing DENV-2 exhibited marked reduction of viral envelope antigen in midguts and salivary glands after infection. DENV-2 titration of individual mosquitoes showed that most Carb77 mosquitoes poorly supported virus replication. Transmission in vitro of virus from the Carb77 line was significantly diminished when compared to control mosquitoes. The presence of DENV-2-derived siRNAs in RNA extracts from midguts of Carb77 and the loss of the resistance phenotype when the RNAi pathway was interrupted proved that DENV-2 resistance was caused by a RNAi response. Engineering of transgenic A. aegypti that show a high level of resistance against DENV-2 provides a powerful tool for developing population replacement strategies to control transmission of dengue viruses.
Limits of Confidence in Tracer Compounds as a Means of Measuring Patient Compliance with Medication (1999)

Chemical tracers are thought to be a desirable means of measuring patient compliance with medication. However, no compound has emerged as a standard. To explain this observation, the authors explored the mathematics of simulated tracers with half-lives 5 to 140 days over a month of daily dosing. To model assay variability, they added random "noise" to calculated tracer serum levels. They then fitted those altered levels to the dosing pattern most likely to have produced them, given known kinetics of the tracers. The large number of possible dosing patterns over a month (268,435,456) magnified uncertainty in interpreting noise-altered levels. At best, with an assay variability of 5%, compliance could be estimated only within 4 to 8 doses per month. The tracer with a half-life of 30 days performed best. Pairing tracers did not improve uncertainty. The authors found that even a model neglecting many real-world variables suggests a limited role for tracers in measuring compliance.
Combined 5-FU and ChoKα Inhibitors as a New Alternative Therapy of Colorectal Cancer: Evidence in Human Tumor-Derived Cell Lines and Mouse Xenografts (2013)

Our data suggest that both drugs in combination display a synergistic antitumoral effect due to ChoKα inhibitors-driven modulation of the metabolization of 5-FU. The clinical relevance of these findings is strongly supported since TCD-717 has recently entered Phase I clinical trials against solid tumors.
Bouncing towards the optimum: Improving the results of Monte Carlo optimization algorithms (1998)

Simulated annealing and related Monte Carlo-type optimization algorithms are used to apply statistical physics concepts, in particular ideas from the statistical mechanics of spin glasses, to find optimal configurations for combinatorial optimization problems. There are formal proofs showing that these algorithms converge asymptotically (i.e.---possibly---for infinitely long simulation times) to a global optimum. Practical implementations, however, only allow for finite simulation times, and, thus, the annealing process is often trapped in energetically higher, suboptimal configurations. In this work we present an algorithm---we call it bouncing--- which takes the final low-energy configuration of, e.g., a conventional monotonically cooled annealing run as an input and subjects it to a schedule of repeatedly reheating and cooling. The maximum of a susceptibility and a specific-heat-like quantity sampled during the initial monotonic cooling process serve as lower and upper starting temperature bounds for this secondary heating and cooling. We present, in addition to a serial implementation, a recipe for a parallel computer, and provide a number of results showing the success of the bouncing method for a particularly prominent example of a combinatorial optimization problem: the traveling salesman problem.
Synthesis and design of parameter extractors for low-power pre-computation-based content-addressable memory using gate-block selection algorithm (2008)

Content addressable memory (CAM) is frequently used in applications, such as lookup tables, databases, associative computing, and networking, that require high-speed searches due to its ability to improve application performance by using parallel comparison to reduce search time. Although the use of parallel comparison results in fast search time, it also significantly increases power consumption. In this paper, we propose a gate- block selection algorithm, which can synthesize a proper parameter extractor of the pre-computation-based CAM (PB-CAM) to improve the efficiency for specific applications such as embedded systems. Through experimental results, we found that our approach effectively reduces the number of comparison operations for specific data types (ranging from 19.24% to 27.42%) compared with the 1's count approach. We used Synopsys Nanosim to estimate the power consumption in TSMC 0.35 um CMOS process. Compared to the 1's count PB-CAM, our proposed PB-CAM achieves 17.72% to 21.09% in power reduction for specific data types.
Correlations of Solution Rheology with Electrospun Fiber Formation of Linear and Branched Polyesters (2004)

The implications of the entanglement concentration (Ce) on the electrospinning process for a series of linear and branched poly(ethylene terephthalate-co-ethylene isophthalate) (PET-co-PEI) copolymers with weight-average molecular weights (Mw) ranging from 11 700 to 106 000 g/mol and branching index values (g‘) from 1.0 to 0.43 were investigated. Analyzing the dependence of specific viscosity (ηsp) on concentration enabled the determination of the semidilute unentangled, semidilute entangled, and concentrated regimes for the PET-co-PEI solutions. Linear and branched copolymers were electrospun from semidilute unentangled, semidilute entangled, and concentrated solutions under identical conditions to determine the effects of concentration regime and molecular topology on electrospun fiber morphology. The dependence of the fiber diameter and morphology on the zero shear rate viscosity (η0) and normalized concentration (C/Ce) was determined. For copolyesters with molecular weights well above the entanglement molecular weight, Ce was the minimum concentration required for electrospinning of beaded fibers, while 2−2.5 times Ce was the minimum concentration required for electrospinning of uniform, bead-free fibers. When the concentration was normalized with Ce, the influence of chain length and topology on the electrospinning process was removed, and the fiber diameter universally scaled with the normalized concentration to the 2.6 power.
TWC-based approach for improving communication reliability in Optical Network-on-Chip (2013)

Optical Network-on-Chip (ONoC) architectures are emerging as a new paradigm to interconnect a large number of processing cores at chip level, thereby enabling to meet the pressing demands for extremely high bandwidth and low power consumption. Some existing ONoC architectures are implemented in an electronic-controlled way in a two-layer 3D chip based on TSV (Through-Silicon Via). However, on-chip thermal effect is an inherent deficiency and chip temperature can fluctuate spatially, which can affect the operation of silicon nanophotonic devices. This leads to significant influence on the reliability of communications. To minimize the thermal impacts and improve the reliability, we propose a novel approach by adding a tunable wavelength converter (TWC) in the router for mesh-based ONoC. Simulation results show that our proposed approach can increase the signal-to-noise ratio (SNR) effectively.
Self-Healing in Distributed Network Environments (2007)

As a new vision in designing computer systems, organic computing is becoming more and more important. These systems are very flexible, autonomous and geared to the organic, human model. They are designed for self-optimizing, self-configuring, self- healing and self-protecting behavior. In the networking area we currently notice a centralization trend resulted in centralized servers, from where an immense number of distributed clients in worldwide locations are being controlled. Because of this centralization process ever fewer administrators are responsible for ever more systems. Concurrently the complexity of software and the dependence on local and distributed systems are growing with each new software version. Therefore it makes sense to apply the organic computing approach to a system that supports this increased requirements, adopting the behavior of human system administrators with the special focus of self-healing.
Carbon-assisted Fabrication of Novel ATO Structures (2007)

Abstract Novel antimony-doped tin oxide (ATO) structures, such as microships and quadrilateral microframes have been prepared by a carbon-assisted method in level tube furnace. Possible growth mechanisms of the as-prepared ATO microstructures are briefly discussed.
The Effect of Staggered PRF's on MTI Signal Detection (1973)

Long-range surveillance radars use MTI techniques to detect moving targets in a clutter background. The transmitter PRF is usually staggered to eliminate the blind speeds due to aliasing of the target and clutter spectra. A spectral analysis of the target and clutter signals is performed for the case of nonuniform sampling, and it is shown that the clutter spectral density continues to fold over at the basic PRF, but the signal spectrum becomes dispersed in frequency, which means that an MTI rader will never be completely blind to moving targets.
RETRACTED: Regulation of prostaglandin production in carrageenan‐induced pleurisy melatonin (1999)

The aim of the present study was to investigate the effect of melatonin on the production of the inflammatory mediator prostaglandins in a model of acute inflammation, carrageenan-induced pleurisy, where prostaglandins are known to play a crucial role. The results show that melatonin (12.5, 25, and 50 mg/kg, intraperitoneally, 15 min before the carrageenan) inhibits the inflammatory response (pleural exudate formation, polymorphonuclear cell infiltration, and prostaglandin production) in a dose-dependent manner. Immunohistochemical examination demonstrated a marked increase in the immunoreactivity to cyclooxygenase-2 (COX-2) in the lungs of carrageenan-treated rats. Pretreatment of carrageenan-treated rats with melatonin (50 mg/kg, intraperitoneally, 15 min before the carrageenan) prevented COX-2 expression. The present results demonstrate that melatonin exerts potent anti-inflammatory effects. Part of these anti-inflammatory effects may be related to a reduction of prostaglandin production during the inflammatory process.
Developmental control of N-CAM sialylation state by Golgi sialyltransferase isoforms (1988)

A rat brain Golgi sialyltransferase activity capable of the differentiation-dependent control of N-CAM sialylation state is described. The specific activity of Golgi sialyltransferase was found to be developmentally regulated with respect to both endogenous and exogenous protein acceptors, with a particular elevation on postnatal days 10-12 when the heavily sialylated or 'embryonic' form of N-CAM is re-expressed. The subsequent developmental decrease in activity was associated with a significant decrease in apparent Km for the CMP-NeuNAc substrate, but not for the asialofetuin exogenous acceptor, which could not be attributed to the temporal expression of an endogenous competitive inhibitor. The apparent Vmax remained constant for CMP-NeuNAc but was significantly reduced for asialofetuin. Sialyltransferase activity, which was optimal at pH 7.0-7.5, was also modulated by various cations. Zinc abolished enzyme function, in contrast to ferric ions which stimulated activity fourfold-sevenfold. The marked activation of the adult form of the enzyme by potassium and magnesium ions, together with the alterations in kinetic constants, suggested this activity to be distinct from that derived from postnatal day-12 tissue. The kinetics of [14C]sialic acid incorporation into immuno-precipitated N-CAM demonstrated the individual polypeptides to be sialylated, possibly by addition of polysialosyl units, in a developmental sequence. The presence of four distinct sialyltransferase activities was demonstrated by non-denaturing gel electrophoresis followed by solid-phase enzyme assay. These isoforms were temporally expressed during development, two being correlated with the postnatal reexpression of the 'embryonic' form of N-CAM.