gebhart/patrepeval huggingface dataset:
Application of the MAIEA Assay to the Kell Blood Group System (1994)

The monoclonal antibody-specific immobilisation of erythrocyte antigens (MAIEA) test has been employed to investigate the Kell system using five monoclonal antibodies which recognise high frequency epitopes on the 93,000-molecular weight Kell glycoprotein: BRIC 18, BRIC 68, BRIC 107, BRIC 203 and 6-22. BRIC 107, which has anti-k-like (KEL2) specificity, identifies a distinct epitope, whilst competitive binding assays suggested that BRIC 203 (anti-Kp^bc), BRIC 18, BRIC 68 and 6-22 (anti K14) comprise an overlapping set of epitopes. The MAIEA assay has been very successful in confirming the assignment of most of the Kell and para-Kell antigens to the Kell protein. Due to the competitive nature of the assay and the fact that the monoclonal antibodies bind to different regions, the results also suggest the relative positions of some of the Kell antigens on the Kell protein; these appear to be located in at least five spatially distinct regions.
Use of a field portable X-Ray fluorescence analyzer to determine the concentration of lead and other metals in soil samples. (1999)

Field portable methods are often needed in risk characterization, assessment and management to rapidly determine metal concentrations in environmental samples. Examples are for determining: "hot spots" of soil contamination, whether dust wipe lead levels meet housing occupancy standards, and worker respiratory protection levels. For over 30 years portable X-Ray Fluorescence (XRF) analyzers have been available for the in situ, non-destructive, measurement of lead in paint. Recent advances made possible their use for analysis of airborne dust filter samples, soil, and dust wipes. Research at the University of Cincinnati with the NITON 700 Series XRF instrument (40 millicurie Cadmium 109 source, L X-Rays) demonstrated its proficiency on air sample filters (NIOSH Method No. 7702, "Lead by Field Portable XRF; limit of detection 6 microg per sample; working range 17-1,500 microg/m3 air). Research with lead dust wipe samples from housing has also shown promising results. This XRF instrument was used in 1997 in Poland on copper smelter area soil samples with the cooperation of the Wroclaw Medical Academy and the Foundation for the Children from the Copper Basin (Legnica). Geometric mean soil lead concentrations were 200 ppm with the portable XRF, 201 ppm with laboratory-based XRF (Kevex) and 190 ppm using atomic absorption (AA). Correlations of field portable XRF and AA results were excellent for samples sieved to less than 125 micrometers with R-squared values of 0.997, 0.957, and 0.976 for lead, copper and zinc respectively. Similarly, correlations were excellent for soil sieved to less than 250 micrometers, where R-squared values were 0. 924, 0.973, and 0.937 for lead, copper and zinc, respectively. The field portable XRF instrument appears to be useful for the determination of soil pollution by these metals in industrial regions.
Learning visual saliency by combining feature maps in a nonlinear manner using AdaBoost (2012)

To predict where subjects look under natural viewing conditions, biologically inspired saliency models decompose visual input into a set of feature maps across spatial scales. The output of these feature maps are summed to yield the final saliency map. We studied the integration of bottom-up feature maps across multiple spatial scales by using eye movement data from four recent eye tracking datasets. We use AdaBoost as the central computational module that takes into account feature selection, thresholding, weight assignment, and integration in a principled and nonlinear learning framework. By combining the output of feature maps via a series of nonlinear classifiers, the new model consistently predicts eye movements better than any of its competitors.
Human contrast discrimination and the threshold of cortical neurons (1987)

The human contrast-discrimination function has a curious shape: In addition to rising for increasing contrasts, both positive and negative, it also rises for very low contrasts on either side of zero. It is shown that this rise near zero contrast is not much affected by procedures that increase or decrease the subject's knowledge of the stimulus; this counts against uncertainty as the immediate cause of the elevation near zero contrast. The alternative explanation in terms of a genuine response threshold is shown to be promising when measurements of human contrast discrimination are compared with values calculated from records of neurons in monkey primary visual cortex. The comparison also suggests that the dynamic range of single neurons is lower than that shown psychophysically. It is suggested that having a cortical threshold may be the visual system's way of preventing false positives when there is much stimulus uncertainty. This threshold may also help to explain why quantum efficiencies calculated from detection thresholds are so poor compared with those estimated from the visual system's susceptibility to added noise.
Synthesis of (−)‐Neplanocin A from (<i>R,R</i>)‐Tartaric Acid (1990)

The synthesis of the carbocyclic nuclcoside analogue 1 from (R,R)-tartaric acid involves eleven steps via intermediary formation of the cyclopentenone 2. Since organolithium compounds other than LiGH2OCH2OCH3 can also be added to 2, any desirable 3-substituted derivatives of 1 are, in principle, accessible in this way. (−)-Neplanocin A is of special importance since it exhibits both antiviral as well as antitumor activity.
4D-QSAR Study of p56<sup>Ick</sup>Protein Tyrosine Kinase Inhibitory Activity of Flavonoid Derivatives Using MCET Method (2011)

A four dimensional quantitative structure activity relationship analysis was applied to a series of 50 flavonoid inhibitors of <TEX>$p56^{lck}$</TEX> protein tyrosine kinase by the molecular comparative electron topological method. It was found that the -log (IC50) values of the compounds were highly dependent on the topology, size and electrostatic character of the substituents at seven positions of the flavonoid scaffold in this study. Depending on the negative or positive charge of the groups correctly embedded in these substituents, three-dimensional bio-structure to increase or decrease -log (IC50) values in the training set of 39 compounds was predicted. The test set of 11 compounds was used to evaluate the predictivity of the model. To generate 4D-QSAR model, the defined function groups and pharmacophore used as topological descriptors in the calculation of activity were of sufficient statistical quality (<TEX>$R^2$</TEX> = 0.72 and <TEX>$Q^2$</TEX> = 0.69). Ligand docking approach by using Dock 6.0. These compounds include many flavonoid analogs, They were docked onto human families of p56lck PTKs retrieved from the Protein Data Bank, 1lkl.pdb.
Tautomerism and <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N NMR spectral assignments of some nitro derivatives of malonic acid diamide (2005)

Because of its reactivity, malonic acid diamide (1) was initially identified as an alternative precursor for the development of a new class of high-density insensitive energetic materials possessing low sensitivity to thermal decomposition and detonation by impact. Nitration of 1 was studied under different conditions and led to three different tautomeric forms (2-4) of nitromalonic acid diamine. Using stronger oxidation conditions the oxadiazole 5 was generated in one step. We report the full 1H, 13C and 15N NMR structural characterization of these compounds in DMSO together with thermal, infrared, mass spectrometric and x-ray analysis. Experimental data obtained for 4 are compatible with an enol-imine form. Our interpretation is consistent with calculated 1H and 13C NMR spectra (ACD).
Internet of Things-Enabled Hospital Wards: Ultrawideband Doctor-Patient Radio Channels (2018)

As ultrawideband (UWB) technology reemerges as a high data-rate solution for the Internet of Things (IoT), we consider the application of technology in medical environments. We present supporting empirical and statistical modeling investigations into body-to-body UWB IoT links for bed-bound patients and roaming clinicians conducting routine medical rounds. The statistical parameters that indicate link reliability are of interest as well as intersymbol interference (ISI) probability for patients in two likely postures with radio antenna locations for the roaming clinician (i.e., the antennas are in a handheld tablet and on the clinician?s waist) to create deployment data for futuristic IoT-enabled hospital environments.
Synthetic experiments in the cycloheptatrienone series. Part III. Syntheses of puberulic acid and isostipitatic acid (1954)

R. B. Johns, A. W. Johnson and J. Murray, J. Chem. Soc., 1954, 198 DOI: 10.1039/JR9540000198
ECa 233 Suppresses LPS-Induced Proinflammatory Responses in Macrophages <i>via</i> Suppressing ERK1/2, p38 MAPK and Akt Pathways (2019)

A current anti-inflammatory agent often targets the prevention of inflammatory disorder development. The standardized Centella asiatica ECa 233 extract has been previously reported for anti-inflammatory effect. This study aimed to investigate its anti-inflammatory effect and mechanisms of ECa 233 in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, nitric oxide (NO) assay, reactive oxygen species (ROS) production assay, enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Our results found that ECa 233 significantly inhibited LPS-stimulated pro-inflammatory mediators production including ROS, NO and prostaglandin E<sub>2</sub> (PGE<sub>2</sub>), and pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α and interleukin (IL)-1β without cytotoxicity. In addition, ECa 233 downregulated not only the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), but also the activation of nuclear factor-kappa B (NF-κB), activated protein kinase B (Akt), extracellular signal-regulated kinase (ERK1/2) and p38 mitogen-activated protein kinases (MAPK) induced by LPS. The inhibition of LPS-induced inflammation due to ECa 233 offered an opportunity as a tentatively potential candidate for the prevention and treatment of inflammatory diseases.
Narrow stripe AlGaAs lasers using double current confinement (1982)

Gain guided AlGaAs lasers in which the current is restricted to flow between two narrow stripes have been fabricated. The double current confinement configuration, which is fabricated by a selective meltback-growth technique, enables the current injection to be restricted to a very narrow section of the active layer. These lasers exhibit very strong antiguiding and operate in many longitudinal modes, which are characteristics of narrow stripe lasers. Potential applications of the twin vertical stripe configuration include arrays of optically coupled lasers and, if a real index waveguiding mechanism can be combined with double current confinement, low threshold lasers.
GATA-3: an unexpected regulator of cell lineage determination in skin (2003)

Multipotent skin stem cells give rise to epidermis and its appendages, including the hair follicle. The Lef-1/Tcf family of Wnt-regulated transcription factors plays a major role in specification of the hair shaft, but little is known about how the equally important hair channel, the inner root sheath (IRS), develops in concert to shape and guide the hair. In a microarray screen to search for transcriptional regulators of hair follicle morphogenesis, we identified GATA-3, a key regulator of T-cell lineage determination. Surprisingly, this transcription factor is essential for stem cell lineage determination in skin, where it is expressed at the onset of epidermal stratification and IRS specification in follicles. GATA-3-null/lacZ knock-in embryos can survive up to embryonic day 18.5 (E18.5), when they fail to form the IRS. Skin grafting unveiled additional defects in GATA-3-null hairs and follicles. IRS progenitors failed to differentiate, whereas cortical progenitors differentiated, but produced an aberrant hair structure. Curiously, some GATA-3-null progenitor cells expressed mixed IRS and hair shaft markers. Taken together, these findings place GATA-3 with Lef-1/Wnts at the crossroads of the IRS versus hair shaft cell fate decision in hair follicle morphogenesis. This newfound function for GATA-3 in skin development strengthens the parallels between the differentiation programs governing hair follicle and lymphocyte differentiation.
Nitric Oxide Mediates Natural Polyphenol-induced Bcl-2 Down-regulation and Activation of Cell Death in Metastatic B16 Melanoma (2006)

Intravenous administration to mice of trans-pterostilbene (t-PTER; 3,5-dimethoxy-4'-hydroxystilbene) and quercetin (QUER; 3,3',4',5,6-pentahydroxyflavone), two structurally related and naturally occurring small polyphenols, inhibits metastatic growth of highly malignant B16 melanoma F10 (B16M-F10) cells. t-PTER and QUER inhibit bcl-2 expression in metastatic cells, which sensitizes them to vascular endothelium-induced cytotoxicity. However, the molecular mechanism(s) linking polyphenol signaling and bcl-2 expression are unknown. NO is a potential bioregulator of apoptosis with controversial effects on Bcl-2 regulation. Polyphenols may affect NO generation. Short-term exposure (60 min/day) to t-PTER (40 microM) and QUER (20 microM) (approximate mean values of the plasma concentrations measured within the first hour after intravenous administration of 20 mg of each polyphenol/kg) down-regulated inducible NO synthetase in B16M-F10 cells and up-regulated endothelial NO synthetase in the vascular endothelium and thereby facilitated endothelium-induced tumor cytotoxicity. Very low and high NO levels down-regulated bcl-2 expression in B16M-F10 cells. t-PTER and QUER induced a NO shortage-dependent decrease in cAMP-response element-binding protein phosphorylation, a positive regulator of bcl-2 expression, in B16M-F10 cells. On the other hand, during cancer and endothelial cell interaction, t-PTER- and QUER-induced NO release from the vascular endothelium up-regulated neutral sphingomyelinase activity and ceramide generation in B16M-F10 cells. Direct NO-induced cytotoxicity and ceramide-induced mitochondrial permeability transition and apoptosis activation can explain the increased endothelium-induced death of Bcl-2-depleted B16M-F10 cells.
Differential expression of the TGF‐β isoforms in embryogenesis suggests specific roles in developing and adult tissues (1992)

The TGF-beta's are multifunctional, pleiotropic molecules with major effects in control of cellular migration, cellular proliferation, and elaboration of extracellular matrix. Thus far, five distinct isoforms of TGF-beta have been described, each approximately 65-85% homologous and containing the characteristic 9 positionally conserved cysteine residues. Although the actions of the activated mature forms of the different isoforms on cells are qualitatively similar in most cases, there are a few examples of distinct activities. For example, TGF-beta's 1 and 3, but not TGF-beta 2, inhibit the growth of large vessel endothelial cells, and TGF-beta's 2 and 3, but not TGF-beta 1, inhibit the survival of cultured embryonic chick ciliary ganglionic neurons. In addition, selective targeting of the latent forms of the TGF-beta's is suggested by the observation that latent TGF-beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye; it is noteworthy in this regard that TGF-beta 2 is unique among various isoforms in that it lacks a RGD integrin-binding sequence in its precursor. The most dramatic differences in the TGF-beta isoforms are seen at the level of expression, where there is now a wealth of data demonstrating both spatially and temporally distinct expression of both the mRNAs and proteins in developing tissues, regenerating tissues, and in pathologic responses.(ABSTRACT TRUNCATED AT 250 WORDS)
Resonant AC‐DC magnetic fields: Calculated response (1988)

An elementary model consisting of one charged particle in a viscous medium exposed to weak ac-dc low-frequency magnetic fields is analyzed to identify and explain the fundamental characteristics of the physical mechanisms that result in a resonance response, which is similar to the familiar cyclotron resonance. The model predicts both frequency and amplitude windows, which are explained in terms of synchronization of the particle with electric fields. Although extrapolation of model results to biological systems is limited by the elementary nature of the model, the model results indicate that observed resonant responses by others of biological systems to ac-dc magnetic fields are probably not due to resonant response of ions in solution, since the model predicts that no resonant response is possible unless the viscous damping is very low, many orders of magnitude lower than the viscous damping of ions in solution.
The Challenge of Productivity Measurement (2006)

In an era of tight budgets and increased outsourcing, getting a good measure of an organization's productivity is a persistent management concern. Unfortunately, experience shows that no single productivity measure applies in all situations for all purposes. Instead, organizations must craft productivity measures appropriate to their processes and information needs. This article discusses the key considerations for defining an effective productivity measure. It also explores the relationship between quality and productivity. It does not advocate any specific productivity measure as a general solution.
Effects of low-frequency stimulation of the subthalamic nucleus on movement in Parkinson's disease (2007)

Excessive synchronization of basal ganglia neural activity at low frequencies is considered a hallmark of Parkinson's disease (PD). However, few studies have unambiguously linked this activity to movement impairment through direct stimulation of basal ganglia targets at low frequency. Furthermore, these studies have varied in their methodology and findings, so it remains unclear whether stimulation at any or all frequencies < or = 20 Hz impairs movement and if so, whether effects are identical across this broad frequency band. To address these issues, 18 PD patients chronically implanted with deep brain stimulation (DBS) electrodes in both subthalamic nuclei were stimulated bilaterally at 5, 10 and 20 Hz after overnight withdrawal of their medication and the effects of the DBS on a finger tapping task were compared to performance without DBS (0 Hz). Tapping rate decreased at 5 and 20 Hz compared to 0 Hz (by 11.8+/-4.9%, p=0.022 and 7.4+/-2.6%, p=0.009, respectively) on those sides with relatively preserved baseline task performance. Moreover, the coefficient of variation of tap intervals increased at 5 and 10 Hz compared to 0 Hz (by 70.4+/-35.8%, p=0.038 and 81.5+/-48.2%, p=0.043, respectively). These data suggest that the susceptibility of basal ganglia networks to the effects of excessive synchronization may be elevated across a broad low-frequency band in parkinsonian patients, although the nature of the consequent motor impairment may depend on the precise frequencies at which synchronization occurs.
VR23: A Quinoline–Sulfonyl Hybrid Proteasome Inhibitor That Selectively Kills Cancer via Cyclin E–Mediated Centrosome Amplification (2015)

The proteasome is clinically validated as a target for cancer therapeutics. However, proteasome-inhibitory agents that are cancer selective have yet to be developed. In this study, we report the identification of a safe and effective proteasome inhibitor with selective anticancer properties. We screened a chemical library constructed using a hybrid approach that incorporated a 4-piperazinylquinoline scaffold and a sulfonyl phamarcophore. From this library, we identified 7-chloro-4-(4-(2,4-dinitrophenylsulfonyl)piperazin-1-yl)quinoline (VR23) as a small molecule that potently inhibited the activities of trypsin-like proteasomes (IC50 = 1 nmol/L), chymotrypsin-like proteasomes (IC50 = 50-100 nmol/L), and caspase-like proteasomes (IC50 = 3 μmol/L). Data from molecular docking and substrate competition assays established that the primary molecular target of VR23 was β2 of the 20S proteasome catalytic subunit. Notably, VR23 was structurally distinct from other known proteasome inhibitors and selectively killed cancer cells by apoptosis, with little effect on noncancerous cells. Mechanistic investigations showed that cancer cells exposed to VR23 underwent an abnormal centrosome amplification cycle caused by the accumulation of ubiquitinated cyclin E. In combinations with the clinically approved chymotrypsin-like proteasome inhibitor bortezomib, VR23 produced a synergistic effect in killing multiple myeloma cells, including those that were resistant to bortezomib. VR23 was effective in vivo in controlling multiple myelomas and metastatic breast cancer cells, in the latter case also enhancing the antitumor activity of paclitaxel while reducing its side effects. Overall, our results identify VR23 as a structurally novel proteasome inhibitor with desirable properties as an anticancer agent.
The Hedgehog-binding proteins Gas1 and Cdo cooperate to positively regulate Shh signaling during mouse development (2007)

Hedgehog (Hh) signaling is critical for patterning and growth during mammalian embryogenesis. Transcriptional profiling identified Growth-arrest-specific 1 (Gas1) as a general negative target of Shh signaling. Data presented here define Gas1 as a novel positive component of the Shh signaling cascade. Removal of Gas1 results in a Shh dose-dependent loss of cell identities in the ventral neural tube and facial and skeletal defects, also consistent with reduced Shh signaling. In contrast, ectopic Gas1 expression results in Shh-dependent cell-autonomous promotion of ventral cell identities. These properties mirror those of Cdo, an unrelated, cell surface Shh-binding protein. We show that Gas1 and Cdo cooperate to promote Shh signaling during neural tube patterning, craniofacial, and vertebral development. Overall, these data support a new paradigm in Shh signaling whereby positively acting ligand-binding components, which are initially expressed in responding tissues to promote signaling, are then down-regulated by active Hh signaling, thereby modulating responses to ligand input.
Do Links Matter? An Investigation of the Impact of Consumer Feedback, Recommendation Networks, and Price Bundling on Sales (2011)

Link structures among items within an E-commerce Web site can be regarded as a potential recommendation that helps new consumers quickly locate relevant products. In this paper, combining a modified version of Google's PageRank method with economic analysis of word of mouth, we investigate whether a product's position within a network composed of recommendation links is incrementally informative about its future sales. Based on data from Amazon.com, we document that with consumer word of mouth and other product characteristics controlled, the position of a product within a recommendation network does influence consumers' purchase decisions, and models incorporating link structure have a higher incremental predictive power of future sales than models without. In addition, as time elapses, the relative weights consumers placed on recommendations with price discount and those without are different. Last, we develop a learning mechanism through which we find the optimal damping value of the PageRank IR model in the Amazon context. Our results show that compared to general Internet surfing behavior, consumer consumption on Amazon is less random. We conclude that even though the product position within a recommendation network does influence customers' purchase behaviors, product sales are still mainly driven by their own product characteristics.