gebhart/patrepeval huggingface dataset:
Bimetallic Ru Electrocatalysts for the OER and Electrolytic Water Splitting in Acidic Media (2010)

We have explored bimetallic Ru–M oxygen evolution reaction (OER) electrocatalysts for use in water splitting in acidic electrolytes. Using an electrochemical multielectrode cell, we investigated the OER activity of selected compositions of seven binary alloy systems, Ru–M . Benchmarked using pure Ru electrocatalysts, Ru–Co, Ru–Ir, and Ru–Cu exhibited improved Ru mass-based catalytic activities. Structural studies of the precursor alloys indicated the presence of hexagonal and cubic mixed metal phases. We hypothesize that the secondary metal component modulates the chemisorption energy of oxygen, which was suggested to be a sensitive rate controlling parameter in the OER catalysis and favors the formation of atomic oxygen and possibly species rather than species on the oxide catalyst.
c-Fos: a Key Regulator of Osteoclast-Macrophage Lineage Determination and Bone Remodeling (1994)

Mice lacking the proto-oncogene c-fos develop the bone disease osteopetrosis. Fos mutant mice were found to have a block in the differentiation of bone-resorbing osteoclasts that was intrinsic to hematopoietic cells. Bone marrow transplantation rescued the osteopetrosis, and ectopic c-fos expression overcame this differentiation block. The lack of Fos also caused a lineage shift between osteoclasts and macrophages that resulted in increased numbers of bone marrow macrophages. These results identify Fos as a key regulator of osteoclast-macrophage lineage determination in vivo and provide insights into the molecular mechanisms underlying metabolic bone diseases.
Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters (2017)

Compartmentation of ancestral gene clusters by AT-rich blocks might promote flexibilities that are evolutionarily advantageous in this fungus' soil habitats and other natural environments. Our analyses, together with the complete genome sequence, provide a better blueprint for biotechnological and industrial applications.
A Simple Method for the Isolation of the Subcomponents of the First Component of Complement by Affinity Chromatography (1974)

Abstract The three subcomponents of the first component of human complement, C1q, C1r and C1s have been separated and purified by a simple method with affinity chromatography on a column of chemically modified agarose to which human IgG has been covalently linked. This method employs human serum as a starting material, and separates active C1 from serum without the need for euglobulin precipitation. Large quantities of hemolytically active and physicochemically and immunochemically homogeneous C1s can be obtained by a subsequent preparative polyacrylamide electrophoresis step. C1q and C1r may be purified by DEAE-cellulose chromatography followed by gel filtration on Sephadex G-200. A fourth protein which was recovered from the affinity column appears to be a subcomponent of the C1 complex.
Nucleic acid conformation diversity: from structure to function and regulation (2001)

Nucleic acids are the structural supports of genetic material and therefore the key factors in many vital cellular processes. The double-stranded right-handed helix is a regular conformation adopted by both DNA and RNA in cells, but an increasing number of results point to the biological importance of alternative structures such as bulges, hairpins, branched junctions or quadruplexes. Progress in the chemical synthesis of oligonucleotides and in the knowledge of the factors that favour a particular conformation has opened new fields of research in molecular recognition and drug design.
Noncollinear parametric generation in LiIO_3 and β-barium borate by frequency-doubled femtosecond Ti:sapphire laser pulses (1995)

In LiIO(3) and BBO crystals the wave-matching conditions for femtosecond noncollinear parametric light generation at lambda = 390 nm pumping wavelength are investigated. In the LiIO(3) crystal simultaneous phase- and group-velocity-matching angles are determined. Parametric generation occurred at 0.45-2.9-mu;m wavelengths by pumping with the second harmonic of 150-fs Ti:sapphire laser pulses and is in qualitative agreement with calculated directions in both crystals.
Preparation and Characterization of Novel Temperature Sensitive Poly(<i>N</i>‐isopropylacrylamide‐<i>co</i>‐acryloyl beta‐cyclodextrin) Hydrogels with Fast Shrinking Kinetics (2004)

Abstract Summary: Novel temperature sensitive poly( N ‐isopropylacrylamide‐ co ‐acryloyl beta‐cyclodextrin) (P(NIPA‐ co ‐A‐CD)) hydrogels with fast shrinking rates were prepared by radical polymerization of NIPA, A‐CD and crosslinker in a mixture of water/1,4‐dioxane as solvent. Because the mixed solvent was a poor solvent for the copolymers, phase separation occurred during the polymerization, which resulted in a heterogeneous, porous structure of the hydrogels. In contrast to the normal PNIPA hydrogel and the homo P(NIPA‐ co ‐A‐CD) gel prepared in water, the P(NIPA‐ co ‐A‐CD) hydrogels synthesized in water/1,4‐dioxane as solvent exhibited higher swelling ratios at the temperature below the lower critical solution temperature (LCST) and shrunk rapidly to equilibrium within shorter time when the temperature was increased above LCST. Increasing the acryloyl beta‐cyclodextrin content in the gels led to a slight decrease of the swelling ratio at lower temperature and had no marked influence on the shrinking kinetics. The gels prepared in water/1,4‐dioxane, at different v/v ratios of 1.0/0.2, 0.8/0.4 and 0.6/0.6, showed similar properties. SEM photos of the heterogeneous P(NIPA‐ co ‐A‐CD) hydrogel prepared in water/1,4‐dioxane. image SEM photos of the heterogeneous P(NIPA‐ co ‐A‐CD) hydrogel prepared in water/1,4‐dioxane.
Regio- and Stereoselective Synthesis of Alkyl Allylic Ethers via Gold(I)-Catalyzed Intermolecular Hydroalkoxylation of Allenes with Alcohols (2008)

Reaction of 1-phenyl-1,2-butadiene with 2-phenyl-1-ethanol catalyzed by a 1:1 mixture of a gold(I) N-heterocyclic carbene complex and AgOTf at room temperature for 1 h led to isolation of (E)-(3-phenethoxy-1-butenyl)benzene in 96% yield as a single regio- and stereoisomer. Gold(I)-catalyzed intermolecular allene hydroalkoxylation was effective for monosubsituted, 1,1- and 1,3-disubstituted, trisubstituted, and tetrasubstituted allenes and for a range of primary and secondary alcohols, methanol, phenol, and propionic acid.
Effective Dimension Reduction Techniques for Text Documents (2010)

Summary Frequent term based text clustering is a text clustering technique, which uses frequent term set and dramatically decreases the dimensionality of the document vector space, thus especially addressing: very high dimensionality of the data and very large size of the databases. Frequent Term based Clustering algorithm (FTC) has shown significant efficiency comparing to some well known text clustering methods, but the quality of clustering still needs further enhancement. In this paper, the morphological variant words, stopwords and grammatical words are identified and removed for further dimension reduction. Two effective dimension reduction algorithms, improved stemming and frequent term generation algorithms have been presented. An experiment on classical text documents as well as on web documents demonstrates that the developed algorithms yield good dimension reduction.
Vertical Stratification of Ammonia in a Broiler House (2008)

The broiler industry is not immune to the problematic nature of NH3 production in animal rearing facilities. Though the headlines of today focus on environmental impact considerations, the detrimental effects of the house air quality on farmers and birds remain considerable for industry viability. This research investigated the vertical stratification of in-house NH3 combined with sampling position down the center of the house and with different NH3 analysis technologies. The results indicated that reuse of litter and house ventilation correlate to trends in NH3 concentration at particular measurement heights. When tunnel ventilation was primary, NH3 concentrations decreased vertically with increasing distance from the litter surface. However, with lower outside temperatures, little ventilation, and a stagnant house atmosphere, no concentration gradient was evident. The work also demonstrated significant variability among professionally calibrated instruments and traditionally used pull tubes. Characterization of interior air quality of broiler houses should consider sampling height to effectively address bird exposure.
Intra-arterial Temozolomide, Osmotic Blood-brain Barrier Disruption and Radiotherapy in a Rat F98-Glioma Model (2017)

Background: The blood-brain barrier (BBB) is a major obstacle in the treatment of brain tumors. Intra-arterial (IA) administration with osmotic BBB disruption (BBBD) allows greater drug delivery to the central nervous system (CNS). Its use with temozolomide (TMZ), the first-line agent in the treatment of glioblastoma, has never been reported in an animal glioma model. Objective: This study was designed to investigate whether increased TMZ delivery would improve survival in the Fischer-F98 glioma model. Methods: Alternative methods of administration for TMZ (200 mg/m2) were tested, comparing intravenous infusions (IV, n=13) to IA (n=13) or BBBD with IA (n=13). IA normal saline (NS) was used as a control group (n=13). Five rats per group also underwent radiotherapy. Survival and adverse effects were measured. TMZ was quantified in plasma, CSF and brain at three time-points post-TMZ infusion (IV, IA or IA+BBBD) by LC-MS\MS. Results: Compared to IV, higher peak TMZ concentrations were observed in brain tumor (BT) tissues with IA and IA+BBBD infusions (4-fold and 5-fold, respectively). No difference in survival was observed between the IA with/without BBBD vs. IV/control groups. Radiotherapy increased survival independently of the method of TMZ delivery. Conclusion: We show that the enhanced delivery of TMZ by IA infusion (with/without BBBD) does not improve survival in this resistant model. However, the method of TMZ administration has a significant impact on CNS delivery. Bypassing the BBB with judicious use of local delivery approaches and appropriate therapeutic agents has potential for the treatment of glioblastomas. Keywords: Blood-brain barrier, Blood-brain barrier disruption, Drug delivery, F98 cells, Glioblastoma multiforme, Temozolomide.
Generalized algebraic scene-based nonuniformity correction algorithm (2005)

A generalization of a recently developed algebraic scene-based nonuniformity correction algorithm for focal plane array (FPA) sensors is presented. The new technique uses pairs of image frames exhibiting arbitrary one- or two-dimensional translational motion to compute compensator quantities that are then used to remove nonuniformity in the bias of the FPA response. Unlike its predecessor, the generalization does not require the use of either a blackbody calibration target or a shutter. The algorithm has a low computational overhead, lending itself to real-time hardware implementation. The high-quality correction ability of this technique is demonstrated through application to real IR data from both cooled and uncooled infrared FPAs. A theoretical and experimental error analysis is performed to study the accuracy of the bias compensator estimates in the presence of two main sources of error.
LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface (2014)

The assembly of lipopolysaccharide (LPS) on the surface of Gram-negative bacterial cells is essential for their viability and is achieved by the seven-protein LPS transport (Lpt) pathway. The outer membrane (OM) lipoprotein LptE and the β-barrel membrane protein LptD form a complex that assembles LPS into the outer leaflet of the OM. We report a crystal structure of the Escherichia coli OM lipoprotein LptE at 2.34 Å. The structure reveals homology to eukaryotic LPS-binding proteins and allowed for the prediction of an LPS-binding site, which was confirmed by genetic and biophysical experiments. Specific point mutations at this site lead to defects in OM biogenesis. We show that wild-type LptE disrupts LPS-LPS interactions in vitro and that these mutations decrease the ability of LptE to disaggregate LPS. Transmission electron microscopic imaging shows that LptE can disrupt LPS aggregates even at substoichiometric concentrations. We propose a model in which LptE functions as an LPS transfer protein in the OM translocon by disaggregating LPS during transport to allow for its insertion into the OM.
SIRT1 is a redox‐sensitive deacetylase that is post‐translationally modified by oxidants and carbonyl stress (2010)

Sirtuin1 (SIRT1) deacetylase levels are decreased in chronic inflammatory conditions and aging where oxidative stress occurs. We determined the mechanism of SIRT1 redox post-translational modifications leading to its degradation. Human lung epithelial cells exposed to hydrogen peroxide (150-250 microM), aldehyde-acrolein (10-30 microM), and cigarette smoke extract (CSE; 0.1-1.5%) in the presence of intracellular glutathione-modulating agents at 1-24 h, and oxidative post-translational modifications were assayed in cells, as well as in lungs of mice lacking and overexpressing glutaredoxin-1 (Glrx1), and wild-type (WT) mice in response to cigarette smoke (CS). CSE and aldehydes dose and time dependently decreased SIRT1 protein levels, with EC(50) of 1% for CSE and 30 microM for acrolein at 6 h, and >80% inhibition at 24 h with CSE, which was regulated by modulation of intracellular thiol status of the cells. CS decreased the lung levels of SIRT1 in WT mice, which was enhanced by deficiency of Glrx1 and prevented by overexpression of Glrx1. Oxidants, aldehydes, and CS induced carbonyl modifications on SIRT1 on cysteine residues concomitant with decreased SIRT1 activity. Proteomics studies revealed alkylation of cysteine residue on SIRT1. Our data suggest that oxidants/aldehydes covalently modify SIRT1, decreasing enzymatic activity and marking the protein for proteasomal degradation, which has implications in inflammatory conditions.
Amplification of altered self-reactive cytolytic T lymphocyte responses by cloned, allospecific human Th cells. (1988)

The effect of a cloned allospecific human Th cell, termed 86, on the in vitro generation of altered self-reactive cytolytic T lymphocytes (CTL) was investigated. Utilizing the induction of hapten altered self-reactive CTL as a model for virus or tumor-specific cell-mediated immunity, we determined that the presence of small numbers of clone 86 cells markedly amplified the generation of hapten altered self-reactive CTL. The killer cells induced belong to the CD4-, CD8+ subset, are specific for the hapten-modified autologous stimulator cells present in culture, and are MHC class I restricted. The CTL induced under these culture conditions are readily expanded in the presence of IL-2 with maintenance of efficient and specific altered self-killing. Of interest, clone 86 cells preferentially enhance the growth of CD8+ T cells and selectively amplify altered self-cytolysis but not NK cell activity. Although in vitro clone 86 cells mediate help for CTL generation via the production of lymphokines (IL-4 but little IL-2), one can envision immunotherapeutic strategies for human disease that involve the adoptive transfer of Th cells functionally analogous to clone 86.
The skin sensitization potential of resorcinol: experience with the local lymph node assay (2007)

Resorcinol is a simple aromatic chemical (1,3-benzenediol) that has found widespread use, particularly as a coupler in hair dyes. Clinical experience clearly shows that resorcinol is a (albeit uncommon) skin sensitizer. By contrast, predictive methods, both animal and human, have previously failed to identify resorcinol as such. Here, we describe the outcome of a recent local lymph node assay performed in accordance with Organisation for Economic Co-operation and Development guideline 429, which correctly identified resorcinol as a skin sensitizer. Clear evidence of a dose response was apparent, and an EC3 value of approximately 6% was calculated. This suggests that the skin-sensitizing potency of resorcinol is approximately 2 orders of magnitude lower than that of p-phenylenediamine but similar to that of hexyl cinnamic aldehyde. These data show the importance of adherence to test guidelines and aligns the clinical experience with resorcinol with that obtained in predictive animal methods.
Phospholipid Vesicle Targeting Using Synthetic Glycolipid and Other Determinants<sup>a</sup> (1985)

The interactions of carbohydrate-modified phospholipid vesicles with various isolated cell types in vitro have been studied to establish a better basis for understanding the mechanisms for recognition and transport of such modified vesicles in vivo. The physical basis for the use of perturbed angular correlation spectroscopy for the measurement of phospholipid vesicle integrity, and the kinetics of uptake of modified phospholipid vesicles by mouse peritoneal macrophage are first reviewed. The effects of variation of the chemical structure of the determinant and other factors indicate that the rate of uptake of cationic vesicles by mouse peritoneal macrophage is directly related to the distance that an amine group can be extended beyond the vesicle surface, and not, for example, to the stereochemistry of the carbohydrate determinant. The uptake mechanism appears to involve generalized phagocytosis and not a receptor-mediated mechanism, or an opsonization process that is not stereospecific.
What You Should Know about Identity Theft (2002)

Identity theft?appropriating someone else?s identity for illicit gain?is the fastest-growing financial crime. It can cause considerable financial losses, and cleaning up a trashed credit history can be time consuming and frustrating. This Economic Commentary examines the identity theft phenomenon?how it works, how lawmakers, regulators, and financial institutions are combating it, and what consumers can do to protect themselves.
Effects of Intact Glucosinolates and Products Produced from Glucosinolates in Myrosinase-Catalyzed Hydrolysis on the Potato Cyst Nematode (<i>Globodera rostochiensis </i>Cv. Woll) (2002)

The potato cyst nematode (Globodera rostochiensis cv. Woll) is responsible for large yield losses in the potato crop, and opportunities for reducing the attack of these plant nematode species are, therefore, important. This study has been devoted to the testing of the in vitro effects on the potato cyst nematode of eight glucosinolates [prop-2-enyl-, but-3-enyl-, (R)-4-methylsulfinylbut-3-enyl-, benzyl-, phenethyl-, 4-hydroxybenzyl-, (2S)-2-hydroxybut-3-enyl-, and (2R)-2-hydroxy-2-phenylethylglucosinolate] as well as the effects of the products of this myrosinase-catalyzed hydrolysis. The glucosinolates were used at three concentrations, 0.05, 0.3, and 1.0 mg/mL, in the presence or absence of the enzyme myrosinase. The effects of the compounds on the mortality were monitored every 8 h for a 72 h period. No effects were found for any of the intact glucosinolates. However, when active myrosinase was included with 1 mg/mL phenethylglucosinolate at pH 6.5, 100% mortality was observed within just 16 h. A similar effect was achieved at the same concentration of benzyl- and prop-2-enylglucosinolates in the myrosinase-containing solutions, although longer exposures were required (24 and 40 h, respectively). The main aglucone products released from the glucosinolates with pronounced effects on the nematodes were shown to be the corresponding isothiocyanates. The results suggest that mixtures of these specific glucosinolates and active myrosinase or autolysis of plant materials containing these enzymes and glucosinolates might be used to control the potato cyst nematode in the soil.
Cerebral and ocular blood flow during α<sub>2</sub>‐blockade: evidence for a modulated sympathetic response (1981)

The effect upon regional blood flows of alpha 2-blockade by yohimbine was investigated in albino rabbits before and during unilateral cervical sympathetic stimulation. The combined effects of i.v. angiotensin, alpha 2-blockade and sympathetic stimulation were also investigated. Regional blood flows were determined using the labelled microsphere method. Yohimbine produced high flow rates in the eyes, the dura, the choroid plexus and in the peripheral organs. Sympathetic stimulation during alpha 2-blockade caused significant blood flow reductions in the brain, the eye and in all other tissues investigated. In the brain the flow reduction was 20-25% in several structures. Angiotensin caused marked reductions in blood flow in the dura and in most peripheral tissues. The effects of sympathetic stimulation were not appreciably changed. The results suggest that vascular alpha 2-receptors affect the vascular tone in some organs and that presynaptic alpha 2-receptors modulate the response to sympathetic stimulation in the brain, the optic nerve and in the choroid plexus.
Modeling Cyclic Deformation of HSLA Steels Using Crystal Plasticity (2004)

High strength low alloy (HSLA) steels, used in a wide variety of applications as structural components are subjected to cyclic loading during their service lives. Understanding the cyclic deformation behavior of HSLA steels is of importance, since it affects the fatigue life of components. This paper combines experiments with finite element based simulations to develop a crystal plasticity model for prediction of the cyclic deformation behavior of HSLA-50 steels. The experiments involve orientation imaging microscopy (OIM) for microstructural characterization and mechanical testing under uniaxial and stress–strain controlled cyclic loading. The computational models incorporate crystallographic orientation distributions from the OIM data. The crystal plasticity model for bcc materials uses a thermally activated energy theory for plastic flow, self and latent hardening, kinematic hardening, as well as yield point phenomena. Material parameters are calibrated from experiments using a genetic algorithm based minimization process. The computational model is validated with experiments on stress and strain controlled cyclic loading. The effect of grain orientation distributions and overall loading conditions on the evolution of microstructural stresses and strains are investigated.
Performance analysis of static versus ARNO converter in Rail AC locomotives (2008)

Single phase to three phase conversion is required to run the auxiliary machines of the AC locomotives of Indian railway. Both ARNO converter and static converters are used on Indian railway to convert single phase alternating supply to three phase alternating supply. This paper describes briefly the ARNO converter and static converter and their relative merits and demerits and then compares the Performance of ARNO converter and static converter provided on 25 KV, 50 Hz AC locomotive of Indian Railway. Annual saving and life cycle power saving by providing static converters is also calculated which prefers use of static converters on AC locomotives than ARNO converter.