gebhart/patrepeval huggingface dataset:
Emicizumab Use in Treatment of Acquired Hemophilia A: A Case Report (2019)

BACKGROUND Acquired hemophilia A (AHA) is a rare hemorrhagic disorder that is caused by producing autoantibodies against factor VIII. It is usually characterized by severe, spontaneous bleeding, which can be life-threatening. The current standard treatments for bleeding prophylaxis are highly effective but accompanied with some disadvantages such as frequent intravenous infusions, high cost, and risk of thromboembolic complications. Emicizumab is a bispecific antibody with a therapeutic FVIII-mimetic nature. Emicizumab has shown a reduction in annualized bleeding rate in congenital hemophilia patients with and without inhibitors. The pathophysiological concepts and preclinical data suggest that Emicizumab can be effectively used for treating AHA. CASE REPORT We present the case of an 87-year-old woman admitted for symptomatic anemia and large chest wall and pelvic hematomas confirmed by imaging, without history of trauma. Her coagulation studies showed isolated prolonged activated partial thromboplastin time (aPTT), low factor VIII activity level, and high levels of factor VIII inhibitor. She was successfully treated with activated prothrombin complex concentrate (aPCC), which was transitioned to Emicizumab on discharge. No recurrent bleeding episodes or adverse events related to Emicizumab were reported during the 2-month follow-up period. CONCLUSIONS A subcutaneous weekly or biweekly injection of Emicizumab, a recombinant monoclonal antibody, offers several advantages: less frequent infusions, good hemostatic efficacy, possible outpatient therapy, and even more cost-effective than bypassing agents. More clinical studies should be conducted to compare Emicizumab with the current standards of care.
Toward Understanding Tryptophan Fluorescence in Proteins (1998)

A general approach to dissecting the complex photophysics of tryptophan is presented and used to elucidate the effects of amino acid functional groups on tryptophan fluorescence. We have definitively identified the amino acid side chains that quench tryptophan fluorescence and delineated the respective quenching mechanisms in a simple model system. Steady-state and time-resolved fluorescence techniques, photochemical H-D exchange experiments, and transient absorption techniques were used to measure individual contributions to the total nonradiative rate for deactivation of the excited state, including intersystem crossing, solvent quenching, and excited-state proton and electron transfer rates. Eight amino acid side chains representing six functional groups quench 3-methylindole fluorescence with a 100-fold range in quenching rate constant. Lysine and tyrosine side chains quench by excited-state proton transfer; glutamine, asparagine, glutamic and aspartic acid, cysteine, and histidine side chains quench by excited-state electron transfer. These studies provide a framework for deriving detailed structural and dynamical information from tryptophan fluorescence intensity and lifetime data in peptides and proteins.
RNA Target Sequences Promote Spreading of RNA Silencing (2003)

It is generally recognized that a silencing-inducing locus can efficiently reduce the expression of genes that give rise to transcripts partially homologous to those produced by the silencing-inducing locus (primary targets). Interestingly, the expression of genes that produce transcripts without homology to the silencing-inducing locus (secondary targets) can also be decreased dramatically via transitive RNA silencing. This phenomenon requires primary target RNAs that contain sequences homologous to secondary target RNAs. Sequences upstream from the region homologous to the silencing inducer in the primary target transcripts give rise to approximately 22-nucleotide small RNAs, coinciding with the region homologous to the secondary target. The presence of these small RNAs corresponds with reduced expression of the secondary target whose transcripts are not homologous to the silencing inducer. The data suggest that in transgenic plants, targets of RNA silencing are involved in the expansion of the pool of functional small interfering RNAs. Furthermore, methylation of target genes in sequences without homology to the initial silencing inducer indicates not only that RNA silencing can expand across target RNAs but also that methylation can spread along target genes.
Rapid Determination of Ampicillin in Bovine Milk by Liquid Chromatography with Fluorescence Detection (1997)

A rapid and sensitive liquid chromatographic (LC) method was developed for the determination of ampicillin residues in raw bovine milk, processed skim milk, and pasteurized, homogenized whole milk with vitamin D. Milk samples were deproteinized with trichloroacetic acid (TCA) and acetonitrile. After centrifugation, the clear supernatant was reacted with formaldehyde and TCA under heat. The major fluorescent derivative of ampicillin was then determined by reversed-phase LC with fluorescence detection. Average recoveries of ampicillin fortified at 5, 10, and 20 ppb (ng/mL) were all > 85% with coefficients of variation < 10%. Limits of detection ranged from 0.31 to 0.51 ppb and limits of quantitation, from 0.66 to 1.2 ppb. After appropriate validation, this method should be suitable for rapid analysis of milk for ampicillin residues at the tolerance level of 10 ppb.
China's New Development Plan: Strategy, Agenda, and Prospects (2007)

In the last five years, China's economy has made great strides. Its annual gross domestic product growth rate averaged 9.4 percent and in the past three years (2003-5) exceeded 10 percent a year. Foreign trade volume has tripled and the foreign exchange reserve exceeded $1 trillion, ranking first in the world. The robust growth, however, has brought concomitant woes such as growing income disparaties, rapid depletion of natural resources, and severe ecological deterioration. The Eleventh Five-Year Plan aims to rectify these shortcomings. This article highlights the key agenda of the new development plan and evaluates the prospect of China's economic and social development in the coming decades.
Efficient Scalable Motion Coding For Wide-Range Scalable Video Compression (2004)

Publication in the conference proceedings of EUSIPCO, Viena, Austria, 2004
Adenosine Deaminase and Thymocyte Maturation (1991)

The congenital absence of adenosine deaminase in humans results in severe combined immunodeficiency. To clarify the process whereby thymocytes are destroyed in the absence of adenosine deaminase activity, we induced a parallel condition in mice through the injection of an inhibitor of adenosine deaminase, deoxycoformycin. We have observed that deoxycoformycin, in addition to maintaining high levels of dATP in thymocytes, blocks the progression of thymocyte differentiation at two points. As a result of the first block, the cortex is depleted of immature cortical thymocytes while CD4+CD8+ thymocytes with functionally rearranged T-cell receptors survive. As a result of the second block, the CD4+CD8+ thymocytes are prevented from further differentiation to mature CD4+CD8- or CD4-CD8+ T lymphocytes and accumulate at the corticomedullar junction and in the medulla. These observations suggest that the maintenance of dNTP pools by adenosine deaminase is critical to at least two stages of thymocyte differentiation.
Malevolent Activity Detection with Hypergraph-Based Models (2017)

We propose a hypergraph-based framework for modeling and detecting malevolent activities. The proposed model supports the specification of order-independent sets of action symbols along with temporal and cardinality constraints on the execution of actions. We study and characterize the problems of consistency checking, equivalence, and minimality of hypergraph-based models. In addition, we define and characterize the general activity detection problem, that amounts to finding all subsequences that represent a malevolent activity in a sequence of logged actions. Since the problem is intractable, we also develop an index data structure that allows the security expert to efficiently extract occurrences of activities of interest.
Infectious Complications of Open Type III Tibial Fractures among Combat Casualties (2007)

Combat-associated type III tibial fractures are predominantly associated with infections due to gram-negative organisms, and these infections are generally successfully treated. Recurrent infections are predominantly due to staphylococci.
Interaction of the Bovine Papillomavirus E6 Protein with the Clathrin Adaptor Complex AP-1 (1998)

The E6 gene of the bovine papillomavirus type 1 (BPV-1) is expressed in fibropapillomas caused by BPV-1 and in tissue culture cells transformed by BPV-1. It encodes one of the two major oncoproteins of BPV-1. In this study, we demonstrate an interaction between the BPV-1 E6 protein and AP-1, the TGN (trans-Golgi network)-specific clathrin adaptor complex. AP-1 is a four-subunit protein complex required for clathrin-mediated cellular transport from the TGN. The AP-1/E6 interaction was observed in vitro and in cells. The E6 binding site on AP-1 was mapped to the N-terminal trunk domain of the gamma subunit. BPV-1 E6 preferentially associated with membrane-bound AP-1 in cells but not with free cytosolic AP-1. BPV-1 E6 was further shown to be recruited to isolated Golgi membranes and to copurify with clathrin-coated vesicles. The recruitment of BPV-1 E6 to Golgi membranes was AP-1 independent, but the E6 interaction with AP-1 was required for its association with clathrin-coated vesicles. Furthermore, AP-1 proteins could compete with BPV-1 E6 for binding to Golgi membranes, suggesting that the recruitment of BPV-1 E6 and AP-1 to Golgi membranes involves a common factor. Taken together, our results suggest that cytosolic BPV-1 E6 is first recruited to the TGN, where it is then recognized by membrane-bound AP-1 and subsequently recruited into TGN-derived clathrin-coated vesicles. We propose that BPV-1 E6, through its interaction with AP-1, can affect cellular processes involving clathrin-mediated trafficking pathway.
Genomic cloud computing: legal and ethical points to consider (2014)

The biggest challenge in twenty-first century data-intensive genomic science, is developing vast computer infrastructure and advanced software tools to perform comprehensive analyses of genomic data sets for biomedical research and clinical practice. Researchers are increasingly turning to cloud computing both as a solution to integrate data from genomics, systems biology and biomedical data mining and as an approach to analyze data to solve biomedical problems. Although cloud computing provides several benefits such as lower costs and greater efficiency, it also raises legal and ethical issues. In this article, we discuss three key 'points to consider' (data control; data security, confidentiality and transfer; and accountability) based on a preliminary review of several publicly available cloud service providers' Terms of Service. These 'points to consider' should be borne in mind by genomic research organizations when negotiating legal arrangements to store genomic data on a large commercial cloud service provider's servers. Diligent genomic cloud computing means leveraging security standards and evaluation processes as a means to protect data and entails many of the same good practices that researchers should always consider in securing their local infrastructure.
Multiresidue Method for Quantitative Determination of Organophosphorus Pesticides in Foods (1985)

A multiresidue method for the quantitative determination of organophosphorus pesticides in foods from the Food and Drug Administration's Total Diet Study is described. The organophosphorus pesticides are separated on the basis of polarity and determined in both fatty and nonfatty foods with a minimum of interferences. The foods analyzed included raw and cooked individual foods as well as combination dishes, water, and whiskey. Recoveries of 17 organophosphorus pesticides in 41 foods ranged from about 80 to 118%.
<i>TBX21</i> : A functional variant predicts improvement in asthma with the use of inhaled corticosteroids (2004)

TBX21 encodes for the transcription factor T-bet (T-box expressed in T cells), which influences naive T lymphocyte development and has been implicated in asthma pathogenesis. Specifically, the T-bet knockout mouse spontaneously develops airway hyperresponsiveness and other changes consistent with asthma. Because airway responsiveness is moderated by the use of inhaled corticosteroids in asthma, it is conceivable that genetic variation in TBX21 may alter asthma phenotypes in a treatment-specific fashion. Here we demonstrate that the nonsynonymous variation in TBX21 coding for replacement of histidine 33 with glutamine is associated with significant improvement in the PC(20) (a measure of airway responsiveness) of asthmatic children in a large clinical trial spanning 4 years. We note that this increase occurs only in the children randomized to inhaled corticosteroids and that it dramatically enhances the overall improvement in PC(20) associated with inhaled corticosteroid usage. The average PC(20) at trial end for subjects on inhaled corticosteroids possessing a variant allele was in the normal range for nonasthmatics. In cellular models, we show that the TBX21 variant increases T helper 1 and decreases T helper 2 cytokine expression comparably with wild type. TBX21 may thus be an important determinant pharmacogenetic response to the therapy of asthma with inhaled corticosteroids.
Distribution of Complement regulators (CD46, CD55 and CD59) in skin appendages, and in benign and malignant skin neoplasms (1992)

Immunohistochemical studies were performed to establish the distribution of membrane cofactor protein (MCP; CD46), decay-accelerating (DAF; CD55) and homologous restriction factor (HRF20; CD59), in normal skin appendages, and in benign and malignant skin neoplasms. At least two of these regulators were detected on normal eccrine glands, apocrine glands and sebaceous glands. They were also found in cellular naevi (CN), seborrhoeic keratoses (SK), basal cell carcinoma (BCC), Bowen's disease (BD), squamous cell carcinoma (SCC) and Paget's disease (PD). Although there were slight differences in their distribution, these regulators were found in all the cells examined, indicating that they are essential factors in human skin as well as other organs, and in neoplasms, in preventing autologous complement attack.
Hardware Minimization of Videotex or Teletext Decoders By Exploitation of Display Redundancy (1984)

The paper shows how, at the various levels of broadcast Teletext or Interactive Videotex service, the natural redundancy inherent in the display has been exploited so that useful savings can be made in the receiving terminals. These savings manifest themselves as simplifications in display chip architecture and as a reduction in the quantity of Display RAM necessary to achieve a given standard of display. The resulting terminal cost reduction can only improve the general acceptance of a given level of service.
Comparison of the transient late region expression of SV40 DNA and SV40-based shuttle vectors: development of a new shuttle vector that is efficiently expressed. (1985)

Transient expression of an SV40-based shuttle vector similar in design to commonly used vectors is shown to be inefficient when compared to expression of SV40 viral DNA. To eliminate this problem, we have designed and constructed a new vector, pSVPiC, which contains a minimal noninterfering plasmid component, the 885-bp plasmid PiAN7, and two SV40 promoter/origin regions. Transient expression of the SV40 late region from pSVPiC is much more efficient than that from previously used vectors and even more efficient than that from SV40 viral DNA. When the gene for rabbit beta-globin is placed in the late region of pSVPiC, it is also expressed at high levels, indicating that this shuttle vector is generally useful for expressing eukaryotic genes.
Alkane C−H Activation and Catalysis by an O-Donor Ligated Iridium Complex (2003)

The first examples of well-defined, O-donor ligated, late-metal complexes that are competent for alkane C-H activation are reported. These complexes exhibit thermal and protic stability and are efficient catalysts for H/D exchange reactions with alkanes.
Rapamycin Impairs Proliferation of Transplanted Islet β Cells (2011)

Rapamycin, at concentration usually used to prevent islet graft rejection, is able to reduce the rate of β-cell proliferation in transplanted rat islets but also in host murine islets. These data suggest that the progressive islet graft dysfunction observed under immunosuppressive therapy may result in part from an impairment of β-cell regeneration.
Thermal Effects in Gain-Guided Diode Laser Arrays (1987)

In recent years, considerable effort has been directed towards the development of high-power gain-guided diode laser arrays. 1 Modeling of these devices has proceeded mostly along the lines of coupled-mode theory, 2 assuming near-threshold conditions so as to render the problem linear. Only very recently have self-consistent model appeared that do not rely on assumptions about inter-stripe coupling. 3-6 The successes of these models include the simulation of beam steering of gain-guided arrays by injection locking, 4 and the prediction of eigenmodes of these arrays of order higher than the number of stripes. 5,6 We present here the results of calculations of the eigenmodes of a ten-stripe gain-guided diode laser array, performed with a numerical model which treats the array as a broad-area resonator. 4 Our model includes the effects of electron diffusion in the active region, gain saturation, and index anti-guiding. We show that the addition of junction heating to the model has a profound effect on both the near- and far-field profiles of the eigenmodes and their modal gains. Indeed, junction heating effects are largely responsible for the appearance of high-order modes in the cw operation of gain-guided arrays.
EEG Asymmetry During Covert Mental Activity (1980)

ABSTRACT Twenty subjects selected for (overt) task‐induced EEG asymmetry performed 17 different covert mental tasks while EEG was recorded from right and left temporoparietal leads. After each task, subjects rated their experiences on four scales: (covert) verbalization, visual imagery, affect, and concentration. Zero‐order and semipartial correlations between integrated EEG measures and subjective ratings showed that verbalization and concentration were systematically related to EEG asymmetry. The results suggest that verbal processes may be a more important influence on temporoparietal EEG asymmetry than are visual imagery processes, at least during purely covert tasks. The results also demonstrate that EEG asymmetry reflects lateralized cognitive processes occurring in the absence of external stimuli or overt responses.
Characterization of highly concentrated antibody solution - A toolbox for the description of protein long-term solution stability (2017)

High protein titers are gaining importance in biopharmaceutical industry. A major challenge in the development of highly concentrated mAb solutions is their long-term stability and often incalculable viscosity. The complexity of the molecule itself, as well as the various molecular interactions, make it difficult to describe their solution behavior. To study the formulation stability, long- and short-range interactions and the formation of complex network structures have to be taken into account. For a better understanding of highly concentrated solutions, we combined established and novel analytical tools to characterize the effect of solution properties on the stability of highly concentrated mAb formulations. In this study, monoclonal antibody solutions in a concentration range of 50-200 mg/ml at pH 5-9 with and without glycine, PEG4000, and Na<sub>2</sub>SO<sub>4</sub> were analyzed. To determine the monomer content, analytical size-exclusion chromatography runs were performed. ζ-potential measurements were conducted to analyze the electrophoretic properties in different solutions. The melting and aggregation temperatures were determined with the help of fluorescence and static light scattering measurements. Additionally, rheological measurements were conducted to study the solution viscosity and viscoelastic behavior of the mAb solutions. The so-determined analytical parameters were scored and merged in an analytical toolbox. The resulting scoring was then successfully correlated with long-term storage (40 d of incubation) experiments. Our results indicate that the sensitivity of complex rheological measurements, in combination with the applied techniques, allows reliable statements to be made with respect to the effect of solution properties, such as protein concentration, ionic strength, and pH shift, on the strength of protein-protein interaction and solution colloidal stability.
Reforming California's Homicide Law (1996)

JUSTICE STATISTICS-1993]. Furthermore, the death penalty and the structure of homicide law are two different topics.Any discussion of capital punishment is best left for another time.In addition, this Article will not discuss the doctrine of justification.There are serious problems with California's law of justification, particularly with self-defense.Justification is a topic worthy of its own article, however, and time and space considerations prevent this Article from giving justification the attention it deserves.3. See infra notes 6-206 and accompanying text.4.
Mixed Sky-Hook and ADD: Approaching the Filtering Limits of a Semi-Active Suspension (2006)

The problem considered in this paper is the design and analysis of control strategies for semiactive suspensions in road vehicles. The most commonly used control algorithm is the well-known sky-hook (SH) damping. Recently, a new control approach named acceleration driven damping (ADD) has been developed, using optimal-control theory. It has been shown that SH and ADD have complementary characteristics: SH provides large benefits around the body resonance; otherwise performs similarly to a passive suspension; instead, ADD provides large benefits beyond the body resonance. The first goal of this paper is to show that—in their specific frequency domains—SH and ADD provide quasi-optimal performances, namely, that it is impossible to achieve (with the same semi-active shock-absorber) better performances. This result has been obtained using the framework of the optimal predictive control, assuming full knowledge of the disturbance. This result is very interesting since it provides a lower-bound to semi-active suspension performances. The second goal of the paper is to develop a control algorithm which is able to mix the SH and ADD performances. This algorithm is surprisingly simple and provides quasi-optimal performances.
Piezoelectric AlGaAs bimorph microactuators (2006)

Piezoelectric bimorph cantilever microactuators based on single-crystal Al0.3Ga0.7As are demonstrated. Fabricated devices are characterized for their quasistatic and resonant behavior when driven in both unimorph and bimorph configurations. Quasistatic actuator behavior is compared with a simple analytic model. Measured unimorph and bimorph tip deflections match well with the model, with deviations resulting from imperfections in device geometry and electrical resistivity of the electrodes. The effects of bimorph actuation on linearity and structural damping at resonance are also evaluated.