gebhart/patrepeval huggingface dataset:
Selection Against Undifferentiated Human Embryonic Stem Cells by a Cytotoxic Antibody Recognizing Podocalyxin-Like Protein-1 (2008)

Future therapeutic applications of differentiated human embryonic stem cells (hESC) carry a risk of teratoma formation by contaminating undifferentiated hESC. We generated 10 monoclonal antibodies (mAbs) against surface antigens of undifferentiated hESC, showing strong reactivity against undifferentiated, but not differentiated hESC. The mAbs did not cross react with mouse fibroblasts and showed weak to no reactivity against human embryonal carcinoma cells. Notably, one antibody (mAb 84) is cytotoxic to undifferentiated hESC and NCCIT cells in a concentration-dependent, complement-independent manner. mAb 84 induced cell death of undifferentiated, but not differentiated hESC within 30 minutes of incubation, and immunoprecipitation of the mAb-antigen complex revealed that the antigen is podocalyxin-like protein-1. Importantly, we observed absence of tumor formation when hESC and NCCIT cells were treated with mAb 84 prior to transplantation into severe combined immunodeficiency mice. Our data indicate that mAb 84 may be useful in eliminating residual hESC from differentiated cells populations for clinical applications. Disclosure of potential conflicts of interest is found at the end of this article.
A 128×128 ultrasonic transducer hybrid array (2002)

Ultrasonic imaging in the low MHz frequency range with large, dense arrays presents many design and fabrication challenges. Conventional ultrasound systems use micro-coaxial cable to connect the array to the front end electronics. While coax technology has improved dramatically in the past decade, interconnecting 16384 array elements with separate wires remains a formidable challenge. In addition to this practical issue, the capacitance of a long coaxial cable (/spl sim/40 pF/m) is much larger than that of a typical 2D array element (<1 pF), creating a voltage divider that severely reduces the signal-to-noise ratio of the channel. A 2D composite piezoelectric receiver array bonded directly to four large custom integrated circuits is described. This 128/spl times/128 (16384 total) element Transducer Hybrid Array (THA) of 200 /spl mu/m unit cell spacing is intended for a 3D real-time imaging system for medical and underwater applications. By reducing the interconnect length to less than 20 /spl mu/m, cable capacitance is no longer a problem. Massively parallel, on-chip signal processing enables true real-time three-dimensional imaging. Favorable tradeoffs using composite piezoelectric materials, enabled by this high-density flip-chip interconnection technology are discussed.
Specification of a configurable general-purpose protocol processor (2002)

A general-purpose protocol processor is specified with a dedicated architecture for protocol processing. The paper defines a functional coverage, analyses the control requirements, and specifies functional pages and a controller unit. The general-purpose protocol processor is for network terminals, and therefore routing is not completely supported. However, it should be possible to use it as part of a router with some minor modifications. The general-purpose protocol processor is partitioned into two parts: a configurable stand-alone part and a program based microcontroller. The configurable part performs the protocol processing without any running program. The processor does not execute any cycle based program; instead execution is controlled by configuration vectors and control vectors. The microcontroller assists with the interface to the host processor and handles the configuration. It is concluded that by partitioning the control into three levels, the architecture is flexible and verification is simplified. The proposed architecture also has higher performance and lower power dissipation than other solutions.
Response of ventilatory and lactate thresholds to continuous and interval training (1985)

The purpose of this study was to evaluate the effects of continuous and interval training on changes in lactate and ventilatory thresholds during incremental exercise. Seventeen males were assigned to one of three training groups: group 1:55 min continuous exercise at approximately 50% maximum O2 consumption (VO2max); group 2: 35 min continuous exercise at approximately 70% VO2max; and group 3: 10 X 2-min intervals at approximately 105% VO2max interspersed with rest intervals of 2 min. All of the subjects were tested and trained on a cycle ergometer 3 day/wk for 8 wk. Lactate threshold (LT) and ventilatory threshold (VT) (in addition to maximal exercise measures) were determined using a standard incremental exercise test before and after 4 and 8 wk of training. VO2max increased significantly in all groups with no statistically significant differences between the groups. Increases (+/- SE) in LT (ml O2 X min-1) for group 1 (569 +/- 158), group 2 (584 +/- 125), and group 3 (533 +/- 88) were significant (P less than 0.05) and of the same magnitude. VT also increased significantly (P less than 0.05) in each group. However, the increase in VT (ml O2 X min-1) for group 3 (699 +/- 85) was significantly greater (P less than 0.05) than the increases in VT for group 1 (224 +/- 52) and group 2 (404 +/- 85). For group 1, the posttraining increase in LT was significantly greater than the increase in VT (P less than 0.05). We conclude that both continuous and interval training were equally effective in augmenting LT, but interval training was more effective in elevating VT.(ABSTRACT TRUNCATED AT 250 WORDS)
Diagnostic testing in obstetrics and gynecology: A clinician's guide (1993)

As we enter a new era of health care in the United States with limited economic resources, there will be an increasing demand for thoughtful and critical evaluation of the diagnostic tests used in clinical medicine. To interpret the clinical utility of diagnostic tests, the physician must understand the fundamental concepts of sensitivity, specificity, and predictive values. The purpose of this paper is to define these terms, demonstrate the use of receiver operating characteristic curves and Bayes' theorem, and provide clinical examples in obstetrics and gynecology to illustrate their use. Practitioners must be able to interpret the results of diagnostic tests to provide accurate information to their patients.(Obstet Gynecol 1993;82:619-23)
Cryopreservation of Medaka Spermatozoa (1997)

In order to establish a reliable and reproducible method for cryopreservation of medaka spermatozoa, we tested several procedures. The vapor phase of liquid nitrogen (LN), the liquid phase of LN, and dry ice were used for freezing, and dimethyl sulfoxide and N, N-dimethylformamide (DMF) were used as cryoprotectants. The best results were obtained using the following method. Medaka spermatozoa were collected in a plastic tube containing 50 μl of fetal bovine serum supplemented with 10% DMF by squeezing an isolated testis. The sperm suspension was frozen by holding the tube for 10 or 20 min in the vapor phase of LN at a depth of 9 or 10 cm from the top of a container. The frozen sample was immersed and stored in LN. After more than one week of storage, the sample in the tube was rapidly thawed by being incubated in a waterbath for 0.5–1 min at 30°C, and then immediately diluted with 2 volumes of Iwamatsu's solution. Fertilization tests using fresh unfertilized eggs showed that the stored spermatozoa could fertilize 96–100% of the eggs. The hatchability of the fertilized eggs was 84-100%. Thus, this study provides, for the first time, a practical method for preserving medaka spermatozoa.
Massenspektrometrische Untersuchungen an Derivaten der Phenylessigsäure, 1. Mitt.: Verlust ortho‐ständiger Substituenten aus Phenylacetamid‐Ionen (1988)

Abstract In den EIMS (70 eV) der ortho‐substituierten Phenylacetamide 2 und 3 treten für X = Cl, Br, NO 2 starke Signale für den Verlust von X‐Radikalen auf, der bei niedrigen Anregungsenergien zur dominierenden bzw. einzigen Reaktion der sehr intensitätsschwachen Molekülionen (< 0.1 %) wird. Die ortho‐ständigen Reste H, CH 3 unf F werden nicht, OCH 3 und CN in nur geringem Maße abgespalten. Der Verlust von o‐Cl, o‐Br und o‐NO 2 ist positionsspezifisch, Abspaltung von meta‐ und para‐ständigem Cl ist nicht nachzuweisen. Zusätzliche Methoxy‐Substitution ( 7 – 9 ) erleichtert die Abspaltung der ortho‐Nitrogruppe. Die MI‐ und CAD‐Spektren der (M‐X) + ‐Ionen werden untersucht.
A novel inward‐rectifying K+ current with a cell‐cycle dependence governs the resting potential of mammalian neuroblastoma cells. (1995)

1. Human and murine neuroblastoma cell lines were used to investigate, by the whole-cell patch-clamp technique, the properties of a novel inward-rectifying K+ current (IIR) in the adjustment of cell resting potential (Vrest), which was in the range -40 to -20 mV. 2. When elicited from a holding potential of 0 mV, IIR was completely inactivated with time constants ranging from 13 ms at -140 mV to 4.5 s at -50 mV. The steady-state inactivation curve (h(V)) was found to be independent of [Na+]o and [K+]o (2-80 mM) and could be fitted to a Boltzmann curve with a steep slope factor of 5-6, and a V1/2 around Vrest. Divalent ion-free extracellular solutions shifted h(V) to the left by about 28 mV. 3. Peak chord conductance, whose maximal value was approximately proportional to the square root of [K+]o, could be fitted to a Boltzmann curve independently of [K+]o, with a V1/2 value around -48 mV and a slope factor of 18. Extracellular Cs+ and Ba2+ blocked the IIR in a concentration- and voltage-dependent manner, but Ba2+ was less effective than it is on classical inward-rectifier channels. 4. Under control culture conditions the values of Vrest and V1/2 of h(V) varied widely among cells. The knowledge of V1/2 proved crucial or the theoretical prediction of Vrest. After cell synchronization in the G0-G1 phase of the cell cycle, or at the G1-S boundaries, the cells reduced their variability of h(V). The same occurred after cell synchronization in G1 by treatment with retinoic acid. 5. The experimental data could be fitted to a classical model of an inward rectifier, after removing the dependence of conductance activation on (V-EK), and incorporating an inactivation with an intrinsic voltage dependence. Moreover, the model predicts, for this novel inward rectifier and in contrast with the classical inward rectifier, the incapacity of maintaining, in physiological media, a Vrest more negative than -35 to -40 mV, which is an important feature of cancer cells.
Treatment of idiopathic clubfoot. A thirty-year follow-up note. (1995)

We evaluated forty-five patients who had seventy-one congenital clubfeet. The average age was thirty-four years (range, twenty-five to forty-two years). Twenty-nine of these patients had been evaluated and reported on in 1980. We performed pedobarographic and electrogoniometric analyses in addition to the clinical and radiographic studies performed previously. With the use of pain and functional limitation as the outcome criteria, thirty-five (78 per cent) of the forty-five patients had an excellent or good outcome compared with eighty-two (85 per cent) of ninety-seven individuals who did not have congenital deformity of the foot. The patient's occupation, passive dorsiflexion as measured with a hand-held goniometer, the antero-posterior calcaneus-fifth metatarsal angle, the total foot pressure time integral, and the number of rapid single-limb toe-ups that could be performed were the only variables that differed significantly between the feet that had an excellent or good result and those that had a poor result (p < 0.05). A comparison of the feet that had an excellent or good outcome with those that had a poor outcome with regard to body-mass index, peak pressure under the heel, and force time integral under the metatarsal heads revealed a p value that was between 0.05 and 0.08 for each variable. The technique of treatment led to good long-term results in our patients who had clubfoot. The data suggest that a sedentary occupation and avoidance of excessive weight gain may improve the over-all long-term result. Excessive weakening of the triceps surae may predispose patients to a poor result; therefore, it is prudent to avoid overlengthening of this muscle. The outcome could not be predicted from the radiographic result.
Breakage and Coalescence Processes in an Agitated Dispersion. Experimental System and Data Reduction (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBreakage and Coalescence Processes in an Agitated Dispersion. Experimental System and Data ReductionF. H. Verhoff, S. L. Ross, and R. L. CurlCite this: Ind. Eng. Chem. Fundamen. 1977, 16, 3, 371–377Publication Date (Print):August 1, 1977Publication History Published online1 May 2002Published inissue 1 August 1977https://pubs.acs.org/doi/10.1021/i160063a012https://doi.org/10.1021/i160063a012research-articleACS PublicationsRequest reuse permissionsArticle Views158Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Effect of Aging on Adhesion Properties of Asphalt Mixtures with the Use of Bitumen Bond Strength and Surface Energy Measurement Tests (2015)

The interaction between asphalt binder and aggregate is fundamental to ensure adequate performance of asphalt mixtures, mainly in the presence of water. The work of adhesion generated between both materials directly affects the resistance of asphalt mixture to moisture damage, because it measures the ease with which water can displace asphalt binder from the aggregate surface. The objective of this study was to characterize the bond strength between asphalt and several aggregate sources. A PG 64-22 neat binder was modified with several additives to determine the effect on adhesion: polymers, nanomaterials, and adhesion promoters. To measure the strength of adhesion, the bitumen bond strength (BBS) test and contact angle measurements between asphalt binder and the aggregate surface by means of goniometry were used. The surface energy of the asphalt and the aggregate, with and without the presence of water, was estimated also. Testing was performed on all binders and on each binder–aggregate combination after ( a) rolling thin-film oven (RTFO) aging and ( b) RTFO and pressure aging vessel aging. The BBS results identified differences in bond strength as a result of moisture conditioning and aging. The differences depended on the aggregate source and binder type. Different failure modes were also observed (i.e., cohesive, adhesive). The results also indicated an increase in strength of adhesion associated with the aging process: the main resistance gain was observed after RTFO aging. Finally, changes in bond strength were compared with functional composition changes associated with the aging process and related to changes in performance.
Genomic DNA recombination with cell‐penetrating peptide‐tagged cre protein in mouse skeletal and cardiac muscle (2014)

The Cre-loxP recombination system has been used to promote DNA recombination both in vitro and in vivo. For in vivo delivery, Cre expression is commonly achieved through the use of tissue/cell type-specific promoters, viral infection, or drug inducible transcription and protein translocation to promote targeted DNA excision. The development of cell permeable (or penetrating) peptide tagged proteins has facilitated the delivery of Cre recombinase protein into cells in culture, organotypic slide culture, or in living animals. In this report, we generated bacterially expressed, his-tagged Cre protein with either a cardiac targeting peptide or an antennapedia peptide at the C-terminus and demonstrated efficient uptake and recombination in both cell culture and mice. To facilitate delivery to cardiac and skeletal muscle, we mixed proteins with pluronic F-127 hydrogel and delivered Cre protein into reporter Rosa26mTmG mouse skeletal muscle or Rosa26LacZ cardiac muscle via ultrasound guided injection. Activation of reporter gene expression indicated that these Cre proteins were enzymatically active. Recombination events were detected only in the vicinity of injection areas. In conclusion, we have developed a method to deliver enzymatically active Cre protein locally to skeletal muscle and cardiac muscle that may be adapted for use with other proteins.
2001: UA Orthophotos (5' Resolution) (2001)

Digital Orthophotos of the UA Campus
Managing Consumer Credit Delinquency in the US Economy: A Multi-Billion Dollar Management Science Application (1992)

GE Capital provides credit card services for a consumer credit business exceeding $12 billion in total outstanding dollars. Its objective is to optimally manage delinquency by improving the allocation of limited collection resources to maximize net collections over multiple billing periods. We developed a probabilistic account flow model and statistically designed programs to provide accurate data on collection resource performance. A linear programming formulation produces optimal resource allocations that have been implemented across the business. The PAYMENT system has permanently changed the way GE Capital manages delinquent consumer credit, reduced annual losses by approximately $37 million, and improved customer goodwill.
An ECFSM-based maximal progress protocol verification (2002)

A number of protocol verification reduction techniques were proposed in the past. Most of these techniques are suitable for verifying communicating protocols specified in the Communicating Finite State Machine (CFSM) model. However, it is impossible to formally specify communicating protocols with predicates and variables using the CFSM model. The Extended Communicating Finite State Machine (ECFSM) model, which incorporates the mechanism for representing variables and predicates, can formally model communicating protocols with variables and predicates. To have more efficient verification for ECFSM-specified protocols, we propose an integrated ECFSM-based global state reduction technique. This new method is based on two techniques: the dead variables analysis which can reduce the number of global states, and the ECFSM-based maximal progress state exploration which can speed up the global state reachability analysis. Using our new ECFSM-based method, the maximal progress protocol verification can be directly applied to the formal description techniques (FDTs) which are based on the extended state transition model, i.e., ISO's Estelle and CCITT's SDL.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Clinical Evaluation of Lentil Lectin-Reactive Alpha-Fetoprotein-L3 in Histology-Proven Hepatocellular Carcinoma (2001)

AFP-L3 is potentially a clinically useful marker for the differentiation of increased AFP levels in hepatocellular carcinoma and chronic liver diseases. The AFP-L3 percentage is closely related to HCC differentiation. We consider the analysis of AFP-L3 a useful adjunct in the diagnosis of HCC.
Visual Fields of the Scuba Diver (1965)

The restricted visual fields available to SCUBA divers were examined by means of an under-water perimetry apparatus. Measurements were obtained for three standard partial masks (covering only the eyes and nose), one atypical “wrap-round” partial mask, and one full-face mask. Data are presented along with some consideration of the interactions between mask design and visual field, and a brief resumé of procedural variables affecting human factors experimentation underwater.
The Diagnostic Value of Complement Fixation in Malaria (1942)

Journal Article The Diagnostic Value of Complement Fixation in Malaria Get access Anna Dean Dulaney, Anna Dean Dulaney From the Departments of Bacteriology, Preventive Medicine, and Medicine University of Tennessee College of Medicine, Memphis Search for other works by this author on: Oxford Academic PubMed Google Scholar Warren K. Stratman-Thomas, Warren K. Stratman-Thomas From the Departments of Bacteriology, Preventive Medicine, and Medicine University of Tennessee College of Medicine, Memphis Search for other works by this author on: Oxford Academic PubMed Google Scholar Otis S. Warr Otis S. Warr From the Departments of Bacteriology, Preventive Medicine, and Medicine University of Tennessee College of Medicine, Memphis Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 70, Issue 3, May 1942, Pages 221–225, https://doi.org/10.1093/infdis/70.3.221 Published: 01 May 1942 Article history Received: 04 September 1941 Published: 01 May 1942
Improvement of Radioimmunotherapy Using Pretargeting (2013)

During the past two decades, considerable research has been devoted to radionuclide therapy using radiolabeled monoclonal antibodies and receptor binding agents. Conventional radioimmunotherapy (RIT) is now an established and important tool in the treatment of hematologic malignancies such as Non-Hodgkin lymphoma. For solid malignancies, the efficacy of RIT has not been as successful due to lower radiosensitivity, difficult penetration of the antibody into the tumor, and potential excessive radiation to normal tissues. Innovative approaches have been developed in order to enhance tumor absorbed dose while limiting toxicity to overcome the different limitations due to the tumor and host characteristics. Pretargeting techniques (pRIT) are a promising approach that consists of decoupling the delivery of a tumor monoclonal antibody (mAb) from the delivery of the radionuclide. This results in a much higher tumor-to-normal tissue ratio and is favorable for therapy as well and imaging. This includes various strategies based on avidin/streptavidin-biotin, DNA-complementary DNA, and bispecific antibody-hapten bindings. pRIT continuously evolves with the investigation of new molecular constructs and the development of radiochemistry. Pharmacokinetics improve dosimetry depending on the radionuclides used (alpha, beta, and Auger emitters) with prediction of tumor response and host toxicities. New constructs such as the Dock and Lock technology allow production of a variety of mABs directed against tumor-associated antigens. Survival benefit has already been shown in medullary thyroid carcinoma. Improvement in delivery of radioactivity to tumors with these pretargeting procedures associated with reduced hematologic toxicity will become the next generation of RIT. The following review addresses actual technical and clinical considerations and future development of pRIT.
Speaker Count application for smartphone platforms (2010)

This paper presents a Speaker Count (SC) algorithm based on pitch estimation designed to recognize single speaker audio recordings from two-speaker recordings. The paper reports the implementation for Symbian OS smartphones. The proposed solution is aimed at limiting implementation complexity and related battery consumption with respect to the schemes available in the literature. This action is mandatory due to the restricted resources available on smart phones. A practical experimental campaign of the proposal is conducted in real smart phones. The Speaker Count method has produced encouraging experimental results. The results also show that the implemented method allows saving energy and acting in a reasonable amount of time.