gebhart/patrepeval huggingface dataset:
Immunoglobulin G antibodies to Helicobacter pylori in patients with dyspeptic symptoms investigated by the western immunoblot technique (1992)

Helicobacter pylori is a gram-negative, curved, rod-shaped bacterium known to cause gastritis and to be an important factor in the pathogenesis of peptic ulcers. Serological testing has recently been proposed as an aid in diagnosis of H. pylori infections. In this study, we used the Western immunoblot technique to evaluate the possibility of using one or more of the antigens from H. pylori for this purpose. Thirteen major bands and about 30 minor bands could be identified by Western blotting when sera from 53 consecutive dyspeptic patients, 27 healthy children, and 25 blood donors were evaluated. Antibodies against most of the major bands were found significantly more frequently in patients with H. pylori infections than in patients without such infections. However, antibodies against a single polypeptide were not produced by all patients with H. pylori infection. Polypeptides with molecular masses of 120, 50, and between 19 and 36 kDa seem to be the most specific polypeptides for the diagnosis of H. pylori infections. This study showed only minor differences in antigenic composition between different clinical isolates of H. pylori, and serological cross-reactions with other bacteria were limited. Major serological cross-reactions were found only with Campylobacter jejuni and with bacterial lipopolysaccharide. However, one band at 60 kDa reacted with antiserum to the Legionella micdadei common antigen, which may indicate a cross-reaction with common antigen from several other bacteria. This study demonstrates that a number of bands may be useful as antigens in serological tests after isolation and purification.
A Study of the Pulsations in a 3/4 Open Jet Wind Tunnel (2002)

<div class="htmlview paragraph">Some open jet wind tunnels have been operating under limitations due to a phenomenon that causes large pressure fluctuations at some speeds. The matter has been studied over several years and no configuration has been found to eliminate these fluctuations without introducing new noise sources.</div> <div class="htmlview paragraph">This paper describes test results from a sub-scale facility that demonstrates this phenomenon. The facility is then tested with a configuration that does not exhibit these phenomena over a wide range of speeds. It is possible to retrofit this configuration in existing facilities.</div> <div class="htmlview paragraph">(The modification is the subject of a pending patent application.)</div>
C-peptide improves adenosine-induced myocardial vasodilation in type 1 diabetes patients (2004)

Patients with type 1 (insulin-dependent) diabetes show reduced skeletal muscle blood flow and coronary vasodilatory function despite intensive insulin therapy and good metabolic control. Administration of proinsulin C-peptide increases skeletal muscle blood flow in these patients, but a possible influence of C-peptide on myocardial vasodilatory function in type 1 diabetes has not been investigated. Ten otherwise healthy young male type 1 diabetic patients (Hb A1c 6.6%, range 5.7-7.9%) were studied on two consecutive days during normoinsulinemia and euglycemia in a double-blind, randomized, crossover design, receiving intravenous infusion of C-peptide (5 pmol.kg-1.min-1) for 120 min on one day and saline infusion on the other day. Myocardial blood flow (MBF) was measured at rest and during adenosine administration (140 microg.kg-1.min-1) both before and during the C-peptide or saline infusions by use of positron emission tomography and [15O]H2O administration. Basal MBF was not significantly different in the patients compared with an age-matched control group, but adenosine-induced myocardial vasodilation was 30% lower (P < 0.05) in the patients. During C-peptide administration, adenosine-stimulated MBF increased on average 35% more than during saline infusion (P < 0.02) and reached values similar to those for the healthy controls. Moreover, as evaluated from transthoracal echocardiographic measurements, C-peptide infusion resulted in significant increases in both left ventricular ejection fraction (+5%, P < 0.05) and stroke volume (+7%, P < 0.05). It is concluded that short-term C-peptide infusion in physiological amounts increases the hyperemic MBF and left-ventricular function in type 1 diabetic patients.
P-selectin, a granule membrane protein of platelets and endothelial cells, follows the regulated secretory pathway in AtT-20 cells. (1992)

P-selectin (PADGEM, GMP-140, CD62) is a transmembrane protein specific to alpha granules of platelets and Weibel-Palade bodies of endotheial cells. Upon stimulation of these cells, P-selectin is translocated to the plasma membrane where it functions as a receptor for monocytes and neutrophils. To investigate whether the mechanism of targeting of P-selectin to granules is specific for megakaryocytes and endothelial cells and/or dependent on von Willebrand factor, a soluble adhesive protein that is stored in the same granules, we have expressed the cDNA for P-selectin in AtT-20 cells. AtT-20 cells are a mouse pituitary cell line that can store proteins in a regulated fashion. By double-label immunofluorescence, P-selectin was visible as a punctate pattern at the tips of cell processes. This pattern closely resembled the localization of ACTH, the endogenous hormone produced and stored by the AtT-20 cells. Fractionation of the transfected cells resulted in the codistribution of P-selectin and ACTH in cellular compartments of the same density. Immunoelectron microscopy using a polyclonal anti-P-selectin antibody demonstrated immunogold localization in dense granules, morphologically indistinguishable from the ACTH granules. Binding experiments with radiolabeled monoclonal antibody to P-selectin indicated that there was also surface expression of P-selectin on the AtT-20 cells. After stimulation with the secretagogue 8-Bromo-cAMP the surface expression increased twofold, concomitant with the release of ACTH. In contrast, the surface expression of P-selectin transfected into CHO cells, which do not have a regulated pathway of secretion, did not change with 8-Br-cAMP treatment. In conclusion, we provide evidence for the regulated secretion of a transmembrane protein (P-selectin) in a heterologous cell line, which indicates that P-selectin contains an independent sorting signal directing it to storage granules.
Role of Hypermutability in the Evolution of the Genus<i>Oenococcus</i> (2007)

Oenococcus oeni is an alcohol-tolerant, acidophilic lactic acid bacterium primarily responsible for malolactic fermentation in wine. A recent comparative genomic analysis of O. oeni PSU-1 with other sequenced lactic acid bacteria indicates that PSU-1 lacks the mismatch repair (MMR) genes mutS and mutL. Consistent with the lack of MMR, mutation rates for O. oeni PSU-1 and a second oenococcal species, O. kitaharae, were higher than those observed for neighboring taxa, Pediococcus pentosaceus and Leuconostoc mesenteroides. Sequence analysis of the rpoB mutations in rifampin-resistant strains from both oenococcal species revealed a high percentage of transition mutations, a result indicative of the lack of MMR. An analysis of common alleles in the two sequenced O. oeni strains, PSU-1 and BAA-1163, also revealed a significantly higher level of transition substitutions than were observed in other Lactobacillales species. These results suggest that the genus Oenococcus is hypermutable due to the loss of mutS and mutL, which occurred with the divergence away from the neighboring Leuconostoc branch. The hypermutable status of the genus Oenococcus explains the observed high level of allelic polymorphism among known O. oeni isolates and likely contributed to the unique adaptation of this genus to acidic and alcoholic environments.
A Nursery Outbreak of Multiple-Aminoglycoside-Resistant<i>Escherichia coli</i> (1984)

In the neonatal intensive care unit (NICU) of one hospital, 16 infants became colonized or infected with multiply-resistant Escherichia coli (MR-E.coli) over an 8-month period. Isolates were obtained from blood, urine, and sputum of three patients and from rectal surveillance cultures of 13 patients. The one patient with the blood isolate died. A matched case-control study identified continuous feeding (nine of 16 cases vs. one of 16 controls, p less than or equal to 0.001) and receipt of aminoglycosides (p less than or equal to 0.03) as risk factors. For case-babies not exposed to continuous feeding, duration of bolus feeding was significantly greater than for their controls (cases, 22 days; controls, 7 days; p less than or equal to 0.02). All 16 isolates were the same serotype and were resistant to amikacin, tobramycin, kanamycin, and gentamicin. The epidemiologic investigation suggested that MR-E. coli may have spread from person-to-person on the hands of personnel and that MR-E. coli persisted in the NICU for 8 months until effective control measures were instituted.
The fluoride-based route to all-silica molecular sieves; a strategy for synthesis of new materials based upon close-packing of guest–host products (2005)

This study surveys the use of a range of structure-directing agents (SDA) in zeolite synthesis experiments under the conditions using HF. The studies made are all for systems containing only silica as the inorganic component. Key points to emerge from the study are a reinforcement of the concept of more open-framework host structures forming when the reactions are more concentrated (lower H 2 O/SiO 2 ratios). This discovery had been reported previously from the research group at ITQ in Valencia, Spain. Our studies show that some novel all-silica compositions have been achieved using this route for synthesis. Two new relationships we explore are whether the use of fluoride anion in synthesis has different nucleation selectivities as a function of dilution, and whether guest molecules can achieve tighter packing in the host structures using the fluoride route as contrasted with the hydroxide route. We explore this with MAS NMR studies. We find that some large organo-cations, which produce no products in alkaline media, give interesting host structure in the fluoride reactions. .
Design and manufacturing considerations of a 0.5 μm CMOS technology on TFSOI (2002)

While thin film SOI (TFSOI) advantages over bulk technology have been reported over the past many years, the TFSOI commodity products are yet to be introduced. Applications of SOI remain in the thick film rad-hard oriented niche market. Theoretical study and silicon implementation of SOI physics and a cost model have for the most part supported the advantages of TFSOI. This paper discusses considerations for the SOI manufacturing feasibility, and the implication to the TFSOI product introduction. The emphasis is placed on the 0.5 /spl mu/m level since this is the state-of-the-art geometry for production of bulk CMOS. Also the huge capital expense for a fabrication line at 0.5 /spl mu/m and beyond will inevitably prolong the technology lifetime and call for value added technology. TFSOI technology at 0.5 /spl mu/m fits into the above category.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Visual-Based Emotional Descriptor and Feedback Mechanism for Image Retrieval * (2006)

A new emotion-based image retrieval method is proposed in this paper. Query emotional descriptors called query color code and query gray code are designed on the basis of human evaluation of 13 emotion pairs (”like-dislike”, ”beautiful-ugly”, ”natural-unnatural”, ”dynamic-static”, ”warm-cold”, ”gay-sober”, ”cheerful-dismal”, ”unstable-stable”, ”light-dark”, ”strong-weak”, ”gaudy-plain”, ”hard-soft”, ”heavy-light”) when 30 random patterns with different color, intensity, and dot sizes are presented. For emotion image retrieval, once a query emotion is selected, the associated query color code and query gray code are obtained, and DB color code and DB gray code that capture color, intensity, and dot size are extracted from each database image. Next, a matching process between the two color codes and between the two gray codes is performed to retrieve images with a sensation of the query emotion. The new relevance feedback method proposed here incorporates the human information needs in the retrieval process by dynamically updating relative weights between the query and DB color codes, and among relative weights of an intra query color code. To show the validity of the proposed method, experiments are performed on over 450 images.
Effect of Injection Rate and Post-Fill Cure Pressure on Properties of Resin Transfer Molded Disks (2002)

The effects of flow rate andpost-fill cure pressure, i.e., packing pressure, on the mechanical properties of resin transfer molded disks are experimentally investigated. An experimental molding setup is constructed to fabricate fiber-reinforced, center-gated, disk-shaped composite parts. Disks are molded at different flow rates and packing pressures in order to observe the effects of these parameters on the mechanical properties andvoidcontent of the final parts. Specimens are cut from three different locations in the molded disks for testing. Specimens from the first two locations are tensile testedto obtain strength and stiffness properties, and the third location is usedfor microscopic analysis to determine void content and void properties. Increased injection rate is found to reduce both the strength and stiffness of the molded parts due to more voids induced by the faster moving fluidfront. Packing pressure is also foundto have a significant effect on specimen properties. At higher packing pressures fewer voids and improved strength andstiffness are observed. Mechanical properties are correlatedwith total void fraction for disks molded at different packing pressures. Exponential decrease in both tensile strength andelastic modulus is observedwith increasing voidfraction. Doubling the voidvolume fraction from 0.35 to 0.72% results in a 15% decrease in strength and a 14% decrease in stiffness. The results demonstrate that selection of suitable injection rate and addition of packing pressure to resin transfer molding (RTM) process can improve mechanical properties of molded parts considerably.
Phase Frequency Detector and Charge Pump for Low Jitter PLL Applications (2018)

In this paper a new technique is presented to improve the jitter performance of conventional phase frequency detectors by completely removing the unnecessary one-shot pulse. This technique uses a variable pulse-height circuit to control the unnecessary one-shot pulse height. In addition, a novel charge-pump circuit with perfect current-matching characteristics is used to improve the output jitter performance of conventional charge pumps. This circuit is composed of a pair of symmetrical pump circuits to obtain a good current matching. As a result, the proposed charge-pump circuit has perfect current-matching characteristics, wide output range, no glitch output current, and no jump output voltage. In order to verify such operation, circuit simulation is performed using 0.18 μm CMOS process parameters.
Financial Literacy Education with an Aboriginal Community: Identifying Critical Moments for Enabling Praxis (2019)

Conventional financial literacy education (FLE) practices promote individual choice and responsibility for financial circumstances. The untruth connected to conventional FLE is that achieving financial well-being is possible after acquiring financial skills and knowledge and choosing to make effective financial decisions. In this article, I share an exploration of FLE practices with an Aboriginal community that unfolded after a conventional train-the-trainer financial literacy workshop failed to gain traction. Nineteen semi-structured interviews took place with community members to understand their experiences, interest and perceived relevance of FLE. The importance of site-specific FLE was revealed as the tension between individual wealth accumulation practices promoted in conventional FLE collided with Indigenous ways of being, knowing and doing. Identified are critical moments for reflection that may enable praxis in FLE. By enabling praxis an educator moves away from conventional one-size-fits-all approaches to FLE, where participants’ needs are assumed, and towards more tailored approaches.
Three fun assignments for an Artificial Intelligence class (2005)

Three creative assignments are discussed that are geared toward an undergraduate Artificial Intelligence curriculum. The modified Turing Test project has students conversing with online chat bots, as well as with other classmates. All conversations are posted on the web and classified as to who the was speaking to, another human or a computer. The sixteen puzzle project requires students to compare the performance of two different heuristics used in solving the sixteen puzzle problem. The final assignment uses LEGO( handy-board based robots to teach the concepts of neural networks.
Construction of Conditional Acid Ceramidase Knockout Mice and<b><i>in vivo</i></b>Effects on Oocyte Development and Fertility (2012)

The number of resting follicles in the ovary and their successful maturation during development define the fertile female lifespan. Oocytes, enclosed within follicles, are subject to natural selection, and the majority will undergo apoptosis during prenatal life through adulthood. Our previous studies revealed high levels of the lipid hydrolase, acid ceramidase (AC), in human and mouse oocytes, follicular fluid and cumulus cells. In addition, supplementation of in vitro fertilization media with recombinant AC enhanced the survival of oocytes and preimplantation embryos. Herein we constructed and used a conditional knockout mouse model of AC deficiency (cACKO) to further investigate the role of this enzyme in oocyte survival in vivo. Immunohistochemical staining, activity assays, and western blot analysis revealed that AC expression was high in the ovaries of normal mice, particularly in the theca cells. After induction of the AC gene knockout with tamoxifen (TM), AC levels decreased in ovaries, and ceramide was correspondingly elevated. A novel immunostaining method was developed to visualize follicles at various stages, and together with light microscopic examination, the transition of the follicle from the secondary to antral stage was found to be defective in the absence of AC. Western blot analysis showed elevated BAX and PARP expression in TM-treated cACKO mouse ovaries compared to control animals. In parallel, the levels of BCL-2 and anti-Mullerian hormone, a marker of ovarian reserve, were decreased. In addition to the above, there was a significant decrease in fertility observed in the TM-treated cACKO mice. Together, these data suggest that AC plays an important role in the preservation of fertility by maintaining low ceramide levels and preventing apoptosis of theca cells, thereby promoting survival of the follicle during the transition from the secondary to antral stage.
Lead-free germanium iodide perovskite materials for photovoltaic applications (2015)

We demonstrate strong potential of computational screening and germanium iodide perovskite compounds for photovoltaic applications.
The Amino Acid Residues at Positions 120 to 123 Are Crucial for the Antigenicity of Hepatitis B Surface Antigen (2007)

The major hydrophilic region (MHR) of hepatitis B surface antigen (HBsAg) harbors conformational B-cell epitopes and is the major target of neutralizing antibodies to HBsAg (anti-HBs). Mutant HBsAg (mtHBsAg) with amino acid substitutions such as G145R is known to affect the binding of specific anti-HB antibodies and their detection by conventional diagnostic assays. In the present study, we focused on the role of the amino acid positions 120 to 123, which are around MHR 2 according to the spectrum of recently identified, naturally occurring mtHBsAg. Strikingly, the amino acid substitution K122I abolished the reactivity of HBsAg in all immunoassays tested so far. Also, mtHBsAg G145R could be clearly detected with four different enzyme-linked immunosorbent assays that were based on monoclonal anti-HB antibodies (MAbs) with high affinity. Positive immunofluorescence staining of mtHBsAg K122I was achieved only by polyclonal anti-HBs, while all MAbs tested failed. mtHBsAg T123N showed a low reactivity in immunoassays and appeared to be secretion defective. The amino acid substitution P120T reduced the binding of anti-HBs but did not completely prevent the detection of mtHBsAg by anti-HB MAbs. The testing of naturally occurring mtHBsAg confirmed that the presence of amino acid substitutions within the region of 120 to 123 is strongly associated with impaired detection in immunoassays. In conclusion, MHR 2 is essential for HBsAg antigenicity, a fact that has not been recognized before.
Co/Ni multilayers with perpendicular magnetic anisotropy: Kerr effect and thermomagnetic writing (1992)

Perpendicular magnetic anisotropy has recently been predicted and confirmed for a Co1/Ni2 multilayer [G. H. O. Daalderop, P. J. Kelly, and F. J. A. den Broeder, Phys. Rev. Lett. 68, 682 (1992)]. This letter reports on some magneto-optical properties of this new multilayer. Its polar Kerr effect appeared to be considerably larger than for a Co1/Pd2 multilayer and comparable to a Co1/Pt2 multilayer. For certain vapor deposition conditions, the perpendicular magneto-optic hysteresis loop of a 120 Å Co1/Ni2 layer showed 100% remanence with a coercivity as high as 140 kA/m. Thermomagnetic writing was possible despite the high Curie temperature.
Radio Frequency Translation for Software Defined Radio (2002)

This chapter contains sections titled: Requirements and Specifications Transmitter Specifications Receiver Specifications Operating Frequency Bands Receiver Design Considerations Basic Considerations Receiver Architectures Dynamic Range Issues and Calculation Adjacent Channel Power Ratio (ACPR) and Noise Power Ratio (NPR) Receiver Signal Budget Image Rejection Filter Functions within the Receiver Transmitter Design Considerations Filtering Analogies between Receiver and Transmitter Transmitter Architectures Transmitter Efficiency and Linearity Candidate Architectures for SDR Zero IF Receivers Quadrature Local Oscillator Variable Preselect Filters Low IF Receivers Conclusions Acknowledgements References Appendix
Inhibitory effect of dibutyryl cAMP on mouse oocyte maturation in vitro (1974)

Abstract The effects of dibutyryl cyclic AMP on the maturation of the mouse oocyte in vitro were studied. It was found that concentrations of 100 μg/ml or greater prevented germinal vesicle breakdown in more than 95% of the treated oocytes. The inhibition of maturation was found to be a reversible phenomenon. When oocytes were exposed to the drug after the completion of germinal vesicle breakdown, no effect of the drug was noted. Thus, dibutyryl cyclic AMP appears to effect only the initial stages of meiotic maturation in the mouse oocyte. Theophylline was also found to inhibit maturation, suggesting that increased levels of cyclic AMP in the oocyte may be the causative agent of the inhibition of maturation.
Wide-Wavelength GaInAs PIN Photodiodes Using a Lattice-Mismatched Light-Absorbing Layer and a Thin InP Cap Layer (1991)

We have fabricated PIN photodiodes (PDs) with a thick lattice-mismatched GaInAs absorbing layer and a thin InP cap layer by metal-organic vapor phase epitaxy (MOVPE). The lattice mismatch and the X-ray FWHM of 2.3-µm GaInAs were 0.5% and 138 arcsec, respectively. These PDs exhibited a very low temperature-dependent response from 1.0 to 1.70 µm with quantum efficiencies as high as 45% at 0.78 µm, 86% at 1.3 µm, 85% at 1.55 µm and 70% at 1.75 µm. A reverse leakage current of 30 nA at -2 V bias was measured, and a -3 dB cutoff frequency of 2.5 GHz was obtained for the 110 µm-dia. PDs.