gebhart/patrepeval huggingface dataset:
Protein-Electroblotting and Microsequencing in Establishing Integrated Human Protein Databases (1991)

Proteins, which are characteristic for a specific state of differentiation, the transformed phenotype or pathological conditions of human cells and tissues were identified by computer analyzed two-dimensional gel electrophoresis. Sequenceable amounts of protein were collected from multiple gels with a gel-concentration device, enabling the elution and concentration of more than twenty protein spots, suspended in 1 ml of sample buffer. The eluted protein was concentrated in a new gel in a very small spot and then electroblotted onto polybase-coated glass-fiber or polyvinylidene-difluoride membranes and in situ digested. The released peptides were separated by micro-bore or narrow-bore reversed phase HPLC and immediately collected on polyethylenimine-coated glass-fiber discs for sequencing. These variations of previously developed methods allowed us to work at higher sensitivity. The procedure is currently being used to try out a systematic analysis of human proteins recovered from two-dimensional gels.
Preliminary Design Concepts for Compliant Mechanism Prosthetic Knee Joints (2004)

Compliant mechanisms present several design advantages that may be exploited in prosthetic joint design — low friction and wear, low part count, light weight, high reliability, efficient manufacturing and assembly, etc. However, they also come with their share of challenges. For example, in the past compliant mechanisms have not been viable alternatives to rigid-body joints in high compression applications. Two principles, isolation and inversion, can be successfully applied to many compliant joints to increase their ability to withstand high compressive loads and make them viable design alternatives to some current prosthetic joint designs. This paper presents preliminary design concepts for a compliant prosthetic knee. The inverted cross-axis flexural pivot design is selected and a proof of concept prototype is constructed and tested. The prototype exceeds the functional specification of 550 lbs, and satisfies a maximum deflection of 140°, an infinite life for a deflection of 110°, and a weight under 2 lbs.
A novel <scp>l</scp>‐glutamate oxidase from <i>Streptomyces endus</i> (1989)

A new flavoenzyme using molecular oxygen to oxidize L-glutamic acid has been purified to homogeneity, as judged by polyacrylamide gel electrophoresis, from the culture medium of Streptomyces endus. Hydrogen peroxide, 2-oxoglutaric acid and ammonia are formed as products. Among 25 amino acids tested including D-glutamic acid, L-glutamine and L-aspartic acid, only L-glutamic acid is converted. The molecular mass of the enzyme was estimated to be about 90 kDa by gel chromatography and 50 kDa by SDS/PAGE. The subunit contains 1 molecule noncovalently bound FAD. The absorption spectrum shows maxima at 273, 355 and 457 nm and the isoelectric point is at pH 6.2. The Km value for L-glutamic acid in air-saturated phosphate pH 7.0 was estimated to be 1.1 mM, the Km for oxygen was calculated to be 1.86 mM at saturating concentration of L-glutamic acid. The enzymic reaction is inhibited by Ag+ and Hg2+ ions. The enzyme described here distinctly differs from two microbial L-glutamate oxidases purified hitherto, with regard to extremely high substrate specificity and to the subunit structure.
High level modeling of an ultra wide-band baseband transmitter in MATLAB (2009)

Wireless personal area network (WPAN) system with ranges of 4-10 m become popular in replacing wire system, technologies such as Bluetooth and ZigBee used for this purpose but due to its low data rate of 1 Mbps which is not enough for large file transfer and applications like video streaming there is a need of technology which address all these issues, Ultra Wideband is such a emerging wireless technology which support data rates up to 0.5 Gbps. UWB with its Multi-Band Orthogonal Frequency Division Multiplexing (MB-OFDM) infrastructure is an ultimate choice for the transmission of high speed data by dividing its wide frequency spectrum into sub 14-bands. The architecture of MB-OFDM transmitter baseband is presented in this paper and its modeling in MATLAB is discussed in detail. Modeling of UWB PHY transmitter baseband in MATLAB is helpful for designing its commercial product by optimizing various parameters.
Noninvasive Tissue Oximetry for Flap Monitoring: An Initial Study (2007)

The ideal monitoring tool to evaluate free flap success should be noninvasive, continuous, and reliable. A new device, the ViOptix Tissue Oximeter (ODISsey) based on near-infrared spectroscopy was evaluated in 30 patients undergoing autologous tissue perforator free flap breast reconstruction with continuous monitoring of the flap during elevation, transfer, and the postoperative period. The device accurately reflected the ischemic drop in oxygen saturation during flap transfer, and the hyperemic response after flow was reestablished. There were no flap failures, but in two patients, the device indicated a venous thrombosis before it was clinically obvious and allowed for expeditious flap salvage. The noninvasive nature of the device, the ability to replace and move the probe in the postoperative period, and the measurement of end organ oxygenation are all advantages over other currently available techniques.
A new method for detecting anaerobic threshold by gas exchange (1986)

Excess CO2 is generated when lactate is increased during exercise because its [H+] is buffered primarily by HCO-3 (22 ml for each meq of lactic acid). We developed a method to detect the anaerobic threshold (AT), using computerized regression analysis of the slopes of the CO2 uptake (VCO2) vs. O2 uptake (VO2) plot, which detects the beginning of the excess CO2 output generated from the buffering of [H+], termed the V-slope method. From incremental exercise tests on 10 subjects, the point of excess CO2 output (AT) predicted closely the lactate and HCO-3 thresholds. The mean gas exchange AT was found to correspond to a small increment of lactate above the mathematically defined lactate threshold [0.50 +/- 0.34 (SD) meq/l] and not to differ significantly from the estimated HCO-3 threshold. The mean VO2 at AT computed by the V-slope analysis did not differ significantly from the mean value determined by a panel of six experienced reviewers using traditional visual methods, but the AT could be more reliably determined by the V-slope method. The respiratory compensation point, detected separately by examining the minute ventilation vs. VCO2 plot, was consistently higher than the AT (2.51 +/- 0.42 vs. 1.83 +/- 0.30 l/min of VO2). This method for determining the AT has significant advantages over others that depend on regular breathing pattern and respiratory chemosensitivity.
Structural and Functional Roles of the Cysteine Residues of Bacillus stearothermophilus Farnesyl Diphosphate Synthase (1994)

p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine or serine. Five mutant enzymes, C73F, C73S, C289F, C289S, and C73S-C289S, were overproduced in Escherichia coli and purified to homogeneity. All of them were active as farnesyl diphosphate synthase, showing specific activities comparable to that of the wild-type enzyme. These results indicate that neither of the cysteines is essential for catalytic function. The C73F mutant, however, was very sensitive to heat treatment, while C73S was as highly stable as the wild type. The Km value of C289F for isopentenyl diphosphate is 10 times that of the wild type. The wild-type enzyme was converted into an oxidized form which was separable from the native enzyme on ion-exchange chromatography, and this conversion was accelerated by cupric ions. Similar conversion has previously been reported by several researchers, who found the occurrence of two forms of pig liver FPP synthase and who attributed this phenomenon to the oxidoreduction of sulfhydryl and disulfide groups. However, even the C73S-C289S mutant, which has no cysteine residues, was also converted into an oxidized form as in the case of the wild-type enzyme. These results provide evidence that residues other than cysteine are involved in the conversion of this enzyme into the oxidized form.
The Multidimensional Physiological Responses to Postconditioning (2010)

Reperfusion is the definitive treatment to reduce infarct size and other manifestations of postischemic injury. However, reperfusion contributes to postischemic injury, and, therefore, reperfusion therapies do not achieve the optimal salvage of myocardium. Other tissues as well undergo injury after reperfusion, notably, the coronary vascular endothelium. Postconditioning has been shown to have salubrious effects on different tissue types within the heart (cardiomyocytes, endothelium) and to protect against various pathologic processes, including necrosis, apoptosis, contractile dysfunction, arrhythmias, and microvascular injury or "no-reflow." The mechanisms by which postconditioning alters the pathophysiology of reperfusion injury is exceedingly complex and involves physiological mechanisms (e.g., delaying re-alkalinization of tissue pH, triggering release of autacoids, and opening and closing of various channels) and molecular mechanisms (activation of kinases) that affect cellular and subcellular targets or effectors. The physiologic responses to postconditioning are not isolated or mutually exclusive, but are interactive, with one response affecting another in an integrated manner. This integrated response on multiple targets differs from the monotherapy approach by drugs that have failed to reduce reperfusion injury on a consistent basis and may underlie the efficacy of this therapeutic approach across species and in human trials.
Hadamard Transform Capillary Electrophoresis (1999)

This paper reports the first demonstration of a multiplex sample injection technique in capillary electrophoresis. The sample was injected into a capillary (effective length, 4 cm) as a pseudorandam Hadamard sequence by a photodegradation technique using a high-power gating laser, and the fluorescence signal, which was measured using a probe excitation beam, was decoded by an inverse Hadamard transformation. The signal-to-noise ratio was improved by a factor of 8, which was in good agreement with the theoretically predicted value of 8.02. This approach is potentially useful for the enhancement of the sensitivity by 3 orders of magnitude in high-resolution capillary electrophoresis, combined with fluorescence detection.
Modification of Gas Chromatographic Substrates for the Separation of Aliphatic Diamines (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTModification of Gas Chromatographic Substrates for the Separation of Aliphatic DiaminesE. D. Smith and R. D. RadfordCite this: Anal. Chem. 1961, 33, 9, 1160–1162Publication Date (Print):August 1, 1961Publication History Published online1 May 2002Published inissue 1 August 1961https://pubs.acs.org/doi/10.1021/ac60177a009https://doi.org/10.1021/ac60177a009research-articleACS PublicationsRequest reuse permissionsArticle Views47Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Improving ranging accuracy of active and passive anchors in the presence of clock imperfection (2013)

When active anchors and agents conduct two-way time-of-arrival (TW-ToA) ranging, passive anchors can help active anchors to improve their localization accuracy. However, the range estimation at both active and passive anchors suffers from error due to clock imperfection. In this paper, we propose a new ranging protocol called two-way time-of-arrival with correction (TW-ToA-C), which can effectively suppress the range estimation error induced by clock imperfection at both active and passive anchors at a cost of transmitting one extra packet at agents.
Bruch's membrane age-related changes vary by region (1987)

Aging changes known to occur in Bruch's membrane may be associated with drusen formation and retinal pigment epithelial mottling, which often have a peripheral as well as a macular distribution. Few details of peripheral Bruch's membrane aging changes have been reported. We conducted a histochemical investigation of Bruch's membrane in 31 postmortem donor eyes and an ultrastructural morphometric investigation of these regional changes in a subgroup of 22 eyes. The age of our donors ranged from 12 days to 80 years. When we reacted 5 micron paraffin embedded sections of chorio-retinal complex fixed in 2% glutaraldehyde-1% paraformaldehyde, we observed increased PAS positivity, and staining with Weigert's elastin and alcian blue at pH 2.5 in Bruch's membranes from donors over 46 years. In this older group, the macula showed increased histochemical reactivity for glycoconjugates, glycosaminoglycans, collagen and elastin as compared with the equator and periphery. Thickening of Bruch's membrane was first detected in the periphery in tissue from donors aged 10 to 45. Thickening of macular Bruch's membrane was first detected in tissue from donors older than 45. The major proportion of thickening, which appears to be due to the deposition of fibrillar and amorphous material, occurred earlier in the inner than the outer collagenous zone. The equatorial region was relatively spared. Our findings confirm and extend the observations of the timing, regional predeliction, and extent of Bruch's membrane aging changes.
45.1 Fabrication of Fast Switchable Patterned Vertical Alignment Mode Using Modified Surface with Reactive Mesogen (2009)

Abstract We proposed a new fast switchable patterned vertical alignment (PVA) mode using UV curable reactive mesogen (RM) which was mixed with vertical alignment layer. In this mixed alignment layer, the pretilt angle was controlled according to an applied voltage during UV exposure for curing the RM. As a result, the response time was dramatically improved at the whole grey levels by controlling the pretilt angle in conventional PVA mode.
Nanomachining of mesoscopic electronic devices using an atomic force microscope (1999)

An atomic force microscope (AFM) is used to locally deplete the two-dimensional electron gas (2DEG) of a GaAs/AlGaAs heterostructure. The depletion is induced by repeated mechanical scribing of the surface layers of the heterostructure using the AFM tip. Measuring the room-temperature resistance across the scribed lines during fabrication provides in situ control of the depletion of the 2DEG. Variation of the room-temperature resistance of such lines tunes their low-temperature characteristics from tunneling up to insulating behavior. Using this technique, an in-plane-gate transistor and a single-electron transistor were fabricated.
Test cost reduction through a reconfigurable scan architecture (2005)

Scan-based designs are widely used to keep test generation complexity within practical limits; nevertheless, scan-based design substantially increases test application time and test data volume. A novel scan-based design is proposed to reduce the test cost. The new scan-design exploits the low specified bit density of the test sets. The circular structure of the proposed architecture enables the use of the captured response of the previously applied pattern as a template for the subsequent pattern while allowing the full observation of the captured response. The functionality provided by the new architecture is utilized to update the template quickly to obtain the next pattern. The experimental results show a substantial reduction in test cost, reaching 90% levels.
Haploidy, Diploidy and Evolution of Antifungal Drug Resistance in Saccharomyces cerevisiae (2004)

We tested the hypothesis that the time course of the evolution of antifungal drug resistance depends on the ploidy of the fungus. The experiments were designed to measure the initial response to the selection imposed by the antifungal drug fluconazole up to and including the fixation of the first resistance mutation in populations of Saccharomyces cerevisiae. Under conditions of low drug concentration, mutations in the genes PDR1 and PDR3, which regulate the ABC transporters implicated in resistance to fluconazole, are favored. In this environment, diploid populations of defined size consistently became fixed for a resistance mutation sooner than haploid populations. Experiments manipulating population sizes showed that this advantage of diploids was due to increased mutation availability relative to that of haploids; in effect, diploids have twice the number of mutational targets as haploids and hence have a reduced waiting time for mutations to occur. Under conditions of high drug concentration, recessive mutations in ERG3, which result in resistance through altered sterol synthesis, are favored. In this environment, haploids consistently achieved resistance much sooner than diploids. When 29 haploid and 29 diploid populations were evolved for 100 generations in low drug concentration, the mutations fixed in diploid populations were all dominant, while the mutations fixed in haploid populations were either recessive (16 populations) or dominant (13 populations). Further, the spectrum of the 53 nonsynonymous mutations identified at the sequence level was different between haploids and diploids. These results fit existing theory on the relative abilities of haploids and diploids to adapt and suggest that the ploidy of the fungal pathogen has a strong impact on the evolution of fluconazole resistance.
A pictorial review of reconstructive foot and ankle surgery: hallux abductovalgus (2015)

This pictorial review focuses on basic procedures performed within the field of podiatric surgery, specifically for the hallux abductovalgus or "bunion" deformity. Our goal is to define objective radiographic parameters that surgeons utilize to initially define deformity, lead to procedure selection and judge post-operative outcomes. We hope that radiologists will employ this information to improve their assessment of post-operative radiographs following reconstructive foot surgeries. First, relevant radiographic measurements are defined and their role in procedure selection explained. Second, the specific surgical procedures of the distal metatarsal, metatarsal shaft, metatarsal base, and phalangeal osteotomies are described in detail. Additional explanations of arthrodesis of the first metatarsal-phalangeal and metatarsal-cuneiform joints are also provided. Finally, specific plain film radiographic findings that judge post-operative outcomes for each procedure are detailed.
Humidity and Gravimetric Equivalency Adjustments for Nephelometer-Based Particulate Matter Measurements of Emissions from Solid Biomass Fuel Use in Cookstoves (2014)

Great uncertainty exists around indoor biomass burning exposure-disease relationships due to lack of detailed exposure data in large health outcome studies. Passive nephelometers can be used to estimate high particulate matter (PM) concentrations during cooking in low resource environments. Since passive nephelometers do not have a collection filter they are not subject to sampler overload. Nephelometric concentration readings can be biased due to particle growth in high humid environments and differences in compositional and size dependent aerosol characteristics. This paper explores relative humidity (RH) and gravimetric equivalency adjustment approaches to be used for the pDR-1000 used to assess indoor PM concentrations for a cookstove intervention trial in Nepal. Three approaches to humidity adjustment performed equivalently (similar root mean squared error). For gravimetric conversion, the new linear regression equation with log-transformed variables performed better than the traditional linear equation. In addition, gravimetric conversion equations utilizing a spline or quadratic term were examined. We propose a humidity adjustment equation encompassing the entire RH range instead of adjusting for RH above an arbitrary 60% threshold. Furthermore, we propose new integrated RH and gravimetric conversion methods because they have one response variable (gravimetric PM2.5 concentration), do not contain an RH threshold, and is straightforward.
Impact of p38 MAP Kinase Inhibitors on LPS-Induced Release of TNF-α in Whole Blood and Primary Cells from Different Species (2015)

Inhibition of TNF-α release by p38 MAPK inhibitors can be reliably identified in well-established laboratory species such as rat or mouse. However, our data indicate that animal models appear to be limited for valid prediction of the inhibitory potential for TNF-α release in humans. Thus, human tissues should be considered early in the drug development process of p38 MAPK inhibitors.
THE EFFECT OF PROLACTIN ON THE SLOUGHING CYCLE IN THE HYPOPHYSECTOMIZED—THYROIDECTOMIZED LIZARD, GEKKO GECKO L. (1971)

SUMMARY Hypophysectomized—thyroidectomized geckos failed to slough during the experimental period of about 120 days. The resting phase of the sloughing cycle persisted so that the outer epidermal generation remained incomplete. These results indicate that one important endocrine component of the sloughing cycle is the normal functioning of the pituitary—thyroid system. Prolactin administration at 0·4 i.u./g body wt on alternate days restored sloughing by reducing the length of the resting phase; the outer generation was completed and was shed after the appearance of a new inner unit.
TFBSTools: an R/bioconductor package for transcription factor binding site analysis (2016)

Supplementary data are available at Bioinformatics online.
Affective Computation Driven Personalization Modeling in Game-Based Learning (2007)

In human-computer interaction and social communication, one's behavior and affective response depend on his personality and environmental stimulus. This means that his behavior and affective response are personalized. In this work, we attempted to build personalization into Game-Based Learning (GBL) system according to user's personality and affective response. User's personality is depicted with a set of attributes such as preference, attitude, character tendency, knowledge & skill, and so on. User's profile is constructed via psychological testing, facial expression recognition, psychophysiology analysis, and behavior analysis. And then, the GBL presents appropriate scenarios and levels adapted to user profile. This will contribute to user's mastery of knowledge & skill, and help user to regulate and improve his personality traits.