gebhart/patrepeval huggingface dataset:
Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth (2017)

Adipose tissue secretes a plethora of adipokines as evidenced by characterization of subcutaneous and visceral adipose tissue secretomes. However, adipose tissue composition and secretion pattern is depot and disease dependent, influencing the adipose tissue secretome. We investigated the secretome of cancer-associated adipose tissue (CAAT) explants from breast cancer patients and explored its role in breast cancer proliferation. CAAT proteins were identified by LC-MS/MS and human protein antibody arrays and stimulated proliferation of three breast cancer cell lines. Kinomics and transcriptomics of MCF-7 breast cancer cells treated with the secretome of CAAT revealed activation of Akt-, ERK- and JNK-pathways and differential expression of activator protein 1 (AP-1) and cAMP responsive element-binding protein (CREB) target genes. The cyclin-dependent kinase (CDK)4/6-inhibitor palbociclib significantly abrogated CAAT-enhanced breast cancer cell proliferation. Our work characterizes the specific breast CAAT protein secretome and reveals its pro-proliferative potency in breast cancer.
The pathology of posttransplant lymphoproliferative disorders occurring in the setting of cyclosporine A-prednisone immunosuppression. (1988)

Posttransplant lymphoproliferative disorders (PTLDs) were diagnosed in 43 patients from the Pittsburgh-Denver series between June 1980 and March 1987. This constitutes a detection rate of 1.7%. Major categories of clinical presentation included a mononucleosislike syndrome, gastrointestinal/abdominal disease, and solid organ disease. The median time of onset in patients initially immunosuppressed with cyclosporine-A (CsA)-containing regimens was 4.4 months after transplant, regardless of tumor clonality. A strong association of PTLD with Epstein-Barr virus (EBV) was observed. A histologic spectrum of lesions from polymorphic to monomorphic was observed. Whereas polymorphic lesions could be either clonal or nonclonal, monomorphic lesions appeared to be clonal in composition. The presence of large atypical cells (atypical immunoblasts) or necrosis did not appreciably worsen the prognosis. Twelve patients had clonal, 13 had nonclonal, and five had both clonal and nonclonal tumors. Clonality was indeterminate in 13 cases. Most patients were treated with a regimen based on reduced immunosuppression and supportive surgery. Almost all nonclonal and about half of the clonal lesions respond to this conservative therapy, indicating that it is an appropriate first line of treatment. This behavior suggests that a spectrum of lesions ranging from infectious mononucleosis to malignant lymphoma constitutes the entity known as PTLD. Some monoclonal tumors can undergo regression, however, apparently in response to host immune control mechanisms. Because of its short latency and strong association with EBV, PTLD is an important model for the study of virus-associated tumor progression in humans.
Recent Developments in Toransition Metal-Catalyzed Polymerization. II. Olefin Polymerization Catalyzed by Low Valent Half-Titanocene Complex. (2002)

3価ハーフチタノセン錯体 [Me2 (3-'Bu-2-MeO-5-MeC6H2) Si (CpMe4) ] TiCl2 (5a) および同配位子を有する4価錯体 [Me2 (3-'Bu-2-MeO-5-MeC6H2) Si (CpMe4) ] TiCl3 (5b) を合成し, 各種共触媒種を組み合わせることにより, オレフィン類の (共) 重合を行った. その結果, Ti金属のd電子数の違いにより, 重合反応速度の経時変化に違いがあることがわかった. さらに, 有機アルミニウム/有機ホウ素塩共触媒系を用いて, エチレン/1-ヘキセン共重合を行った場合に, 本触媒系は共重合性比が用いる有機アルミ種によって変化することがわかり, 有機アルミニウム種が, 重合進行中にも活性点近傍に存在している可能性が示唆された. また, 本錯体は配位不飽和度を減らすために, 配位子上のエーテル部位がTi原子に配位することで, より安定化することが, ab initio量子化学計算により確認された.
An efficient skew-insensitive algorithm for join processing on grid architectures (2011)

Scientific experiments in many domains generate a huge amount of data whose size is in the range of hundreds of megabytes to petabytes. These data are stored on geographically distributed and heterogeneous resources. Researchers who need to analyze and have a fast access to such data are also located all over the globe. Queries executed by these researchers may require the transfer of huge amount of data over the wide area network in a reasonable time. Due to these emerging needs, the grid infrastructure was born. In this paper, we are interested in treating join queries on the grid. We propose a new parallel algorithm allowing to reduce communication and disk Input/Output costs to minimum. This algorithm guarantees a balanced load among all processing nodes in each cluster and then among all the clusters of a grid architecture.
GENETIC COMPONENT IN THE INFLAMMATORY RESPONSE INDUCED BY BACTERIAL LIPOPOLYSACCHARIDE (1998)

Multiple organ dysfunction syndrome (MODS) appears to be the result of a complex program influenced by multiple factors, including environmental, physiological, and immunological conditions. Thus, an uncontrolled inflammatory response following a stochastic event, the initial injury, is believed to be the cause for the development of this syndrome. Several lines of evidence suggest that a genetic component could contribute to the regulation of the inflammatory response, as well, but no direct evidence demonstrates a heritable predisposition to MODS. In the present study, a genetic contribution was demonstrated for the inflammatory response induced by the administration of bacterial lipopolysaccharide (LPS) in different, genetically distinct strains of inbred mice. A survey of five inbred strains showed that mortality following administration of Escherichia coli LPS (20 mg/kg) was highest in C57BL/6J (B6) mice, while A/J mice were the most resistant. Accordingly, B6 and A/J mice were examined further for differences in the inflammatory response elicited by LPS. B6 mice showed higher levels of circulating interleukin-1beta and interleukin-6, as well as higher mRNA levels of hepatic beta-fibrinogen (an acute-phase gene) and metallothionein. Surprisingly, the circulating levels of tumor necrosis factor-alpha were significantly higher in A/J than in B6 mice after LPS administration. Since B6 and A/J mice were bred and raised in identical environments and received the same LPS challenge, the contrasting inflammatory response that was observed is largely attributable to genetic differences between these two strains. These data illustrate that the response to injury could be modulated by the genetic background of the individual. This information may be pertinent for the care of critically ill patients.
A new synthesis of 7H-pyrido(1,2,3-de)(1,4)benzoxazine derivatives including an antibacterial agent, ofloxacin. (1986)

A new method for the synthesis of 7H-pyrido[1, 2, 3-de][1, 4]benzoxazine derivatives was developed. The method is characterized by the intramolecular cyclization of 1-(1-hydroxyprop-2-yl)-8-fluoro-4-quinolones which are prepared in three or four steps from ethyl 2, 3, 4, 5-tetrafluoro-benzoylacetate. As an application of this method, ofloxacin, an antibacterial agent, was synthesized.
Restriction endonuclease fingerprinting by SSCP (REF), an efficient method of screening for mutations in long contiguous segments of DNA (1994)

Dideoxy fingerprinting is an efficient method of screening for the presence of mutations in short exons ({le}250 bp). Long contiguous segments can be screened by sequential ddF reactions. To screen long contiguous segments in a more rapid manner, REF has been developed. REF will be described in the context of a model system in exon H of the factor IX gene. A 1 kb segment is PCR amplified and digested with each of five groups of restriction endonucleases. The endonucleases are chosen such that, in each group, the average size of the fragments is about 150 bp. After digestion, the products are mixed, 5{prime} end-labeled with T4 polynucleotide kinase, boiled, and electrophoresed under nondenaturing conditions. Each lane screens 1 kb and contains 70 segments (7 fragments per digestion x 5 digestions x 2 strands). The matrices tested were 5.6% polyacrylamide (PA) and 7.5% GeneAmp{sup {trademark}} (GA) at temperatures of either 23{degrees}C (RT) or 8{degrees}C (LT). Point mutations resulted in the gain or loss of a restriction site in 21% of 24 test mutations. In addition, mutations could be detected if any of 5 restriction fragments with the same mutation (producing 10 denatured segments) displayed abnormal mobility (SSCP component). The averagemore » sensitivity per segment of the SSCP component for the 24 point mutations ranged from 49% for PA at RT to 68% with GA at LT. REF detected 96% of the mutations with PA at RT and 100% with GA at RT or LT. These latter two conditions detected 100% of a subsequent blinded sample that contained normal controls and 27 different mutations. A blinded analysis is in progress to determine the sensitivity of REF when the segment size is 2 kb.« less
State-space exploration for concurrent algorithms under weak memory orderings (2008)

Several concurrent implementations of familiar data abstractions such as queues, sets, or maps typically do not follow locking disciplines, and often use lock-free synchronization to gain performance. Since such algorithms are exposed to a weak memory model, they are notoriously hard to get correct, as witnessed by many bugs found in published algorithmsWe outline a technique for analyzing correctness of concurrent algorithms under weak memory models, in which a model checker is used to search for correctness violations. The algorithm to be analyzed is transformed into a form where statements may be reordered according to a particular weak memory ordering. The transformed algorithm can then be analyzed by a model-checking tool, e.g., by enumerative state exploration. We illustrate the approach on a small example of a queue, which allows an enqueue operation to be concurrent with a dequeue operation, which we analyze with respect to the RMO memory model defined in SPARC v9.
Alterations in the alveolar epithelium after injury leading to pulmonary fibrosis. (1996)

This review discusses current knowledge of the involvement of the alveolar epithelium in tissue remodelling during fibrogenesis. The purpose of the present paper is to give an overview, including the authors' own results, of knowledge of ultrastructural alterations, proliferation kinetics and phenotypic changes of pneumocytes in experimental and clinical pathology of pulmonary fibrosis. After lung injury, the alveolar epithelial cells show ultrastructural alterations, hypertrophy and hyperplasia, and a modulation of a series of structural and membrane proteins such as cytoskeletal changes, loss or de novo expression of epithelial adhesion molecules, and altered lectin binding. Furthermore, enhanced secretion of proteases, of cytokines and other soluble factors can be observed in the alveolar epithelium. These findings suggest the contribution of the epithelium in the remodelling process to be greater than expected. Estimations of the cell kinetics show that type II pneumocytes have the proliferative capacity to restore high proportions of damaged type I cells within few hours. In fibrosis this capacity also seems to be affected seriously, resulting in transitional phenotypes between type II and type I cells. Additionally, in the light of the detection of CD44 type of adhesion molecules at the foot processes of type II pneumocytes, some aspects of epithelial-fibroblast interaction are described.
A Novel Lectin with Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Dried Fruiting Bodies of the Monkey Head Mushroom<i>Hericium erinaceum</i> (2010)

A lectin designated as Hericium erinaceum agglutinin (HEA) was isolated from dried fruiting bodies of the mushroom Hericium erinaceum with a chromatographic procedure which entailed DEAE-cellulose, CM-cellulose, Q-Sepharose, and FPLC Superdex 75. Its molecular mass was estimated to be 51 kDa and its N-terminal amino acid sequences was distinctly different from those of other isolated mushroom lectins. The hemagglutinating activity of HEA was inhibited at the minimum concentration of 12.5 mM by inulin. The lectin was stable at pH 1.9-12.1 and at temperatures up to 70 degrees C, but was inhibited by Hg(2+), Cu(2+), and Fe(3+) ions. The lectin exhibited potent mitogenic activity toward mouse splenocytes, and demonstrated antiproliferative activity toward hepatoma (HepG2) and breast cancer (MCF7) cells with an IC(50) of 56.1 microM and 76.5 microM, respectively. It manifested HIV-1 reverse transcriptase inhibitory activity with an IC(50) of 31.7 microM. The lectin exhibited potent mitogenic activity toward murine splenocytes but was devoid of antifungal activity.
Charlotte: Metacomputing on the Web (1999)

Parallel computing on local area networks is generally based on mechanisms that specifically target the properties of the local area network environment. However, these mechanisms do not effectively extend to wide area networks due to issues such as heterogeneity, security, and administrative boundaries. We present a system which enables application programmers to write parallel programs in Java and allows Java-capable browsers to execute parallel tasks. It comprises a virtual machine model which isolates the program from the execution environment, and a runtime system realizing this virtual machine on the Web. Load balancing and fault masking are transparently provided by the runtime system.
Circuit Analysis of Photosensitive Capacitance in Semi-Insulating GaAs (2013)

We describe a circuit model for photosensitive capacitance in bulk semi-insulating GaAs toward tunable resonant applications. Capacitances from two separate regions are considered to interpret experimental results. A smaller valued capacitance exists between the depletion edges within the bulk material. Photodoping in this region progressively shorts out the bulk capacitance, leaving only the higher valued depletion capacitance. The depletion capacitance also increases with illumination, and numerical simulation is used to aid interpretation. Thus, the series combination of capacitance can be optically varied over orders of magnitude. Our results indicate that capacitance is nearly independent of applied voltage over a usable parameter space, making the concept attractive for linear application.
Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic AcidsSatoshi. Fujita, Masayoshi. Momiyama, Yasumitsu. Kondo, Naoto. Kagiyama, Samuel H. Hori, and Takeshi. ToruCite this: Anal. Chem. 1994, 66, 8, 1347–1353Publication Date (Print):April 15, 1994Publication History Published online1 May 2002Published inissue 15 April 1994https://pubs.acs.org/doi/10.1021/ac00080a022https://doi.org/10.1021/ac00080a022research-articleACS PublicationsRequest reuse permissionsArticle Views267Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Study on complex formation between recombinant human thrombomodulin fragment and thrombin using surface plasmon resonance (2000)

Human thrombomodulin (hTM) is a newly described endothelial cell associated protein that functions as a potent natural anticoagulant by converting thrombin from a procoagulant protease to an anticoagulant. The affinity constant of recombinant human soluble TM (rhs-TM) and a peptide containing active site of hTM fragment (f-hTM) with thrombin were determined using the surface plasmon resonance. The interaction of f-hTM with thrombin could be analyzed by a simple model, whereas the association and the dissociation steps of rhs-TM with thrombin consisted of at least two kinds of interaction phases. The dissociation constant for complex (K(D)) of f-hTM and thrombin was determined to be 205 nM, which was more than twice as high as that of rhs-TM (6.7 and 75 nM). The lower affinity of f-hTM was not due to the slow association rate but to the rapid dissociation rate. It comes clear that f-hTM interacts with thrombin rapidly.
State estimation of uncertain multiple model with unknown inputs (2004)

This paper is dedicated to the synthesis of a sliding mode multiple observer. The considered systems are represented by an uncertain (nonlinear) multiple model with unknown inputs. Stability conditions of such observers are expressed in terms of linear matrix inequalities (LMI). An example of simulation is given to illustrate the proposed method.
Measuring users' privacy payoff using intelligent agents (2009)

As many people are now taking advantages of on-line services, the value of the private data they own comes into sight as a problem of fundamental concern. This paper takes the position that, individuals are entitled to secure control over their personal information, disclosing it as part of a transaction only when they are fairly compensated. To make this a concrete possibility, users require technical instruments to be able to measure their privacy payoff and track the use of their private data. In this paper, we propose an intelligent agent-based framework for privacy payoff measurements and negotiation. Intelligent agents in our system collaboratively work on behalf of users for the goal of maximizing their benefit and protect the use of their private data. The overall framework is described, and a particular simulation experiment is presented to evaluate our approach.
Copper Coated Silica Nanoparticles for Odor Removal (2010)

Copper species coated silica nanoparticles (CuOXS) were synthesized for odor removal application. Coating with copper increased the capacity of silica nanoparticles for eliminating a model odor-ethyl mercaptan. Surface area, pore size distribution, and electron paramagnetic resonance spectroscopy analyses indicated that, at lower copper concentrations, copper species preferentially adsorb in 20 Å pores of silica. These copper species in a dispersed state are effective in catalytic removal of ethyl mercaptan. The best performance of copper-coated silica nanoparticles was achieved at a copper concentration of 3 wt %, at which all 20 Å nanopores were filled with isolated copper species. At higher copper loading, copper species are present as clusters on silica surfaces, which were found to be less effective in removing ethyl mercaptan. Gas chromatography experiments were carried out to verify catalytic conversion of ethyl mercaptan to diethyl disulfide by CuOXS particles. The present study suggests that the nature of the copper species and their site of adsorption, as well as state of dispersion, are important parameters to be considered for catalytic removal of sulfur-containing compounds. These parameters are critical for designing high-performance catalytic copper-coated silica nanoparticles for applications such as deodorization, removal of sulfur compounds from crude oil, hydrogenation, and antimicrobial activity.
ENERGY AND MOMENTUM TRANSFER BY NEUTRINO RADIATION TO THE MANTLE OF COLLAPSING STAR AND SUPERNOVA EXPLOSIONS (1975)

Different mechanisms of momentum and energy transfer to mantles of massive collapsing stars are discussed.
The influence of external fixators on fracture motion during simulated walking (1996)

This experimental study examines the relative influence of five unilateral external fixators on tibial fracture stability during simulated walking. Stability during routine patient activity is important, because cyclic inter fragmentary motion, or strain, has been shown to affect fracture healing. In model stable fractures simulating early healing (six weeks), it was found that fixators do little to constrain against axial inter fragmentary strains as great as 100% at only nominal weight-bearing (6.0 kg). These strains may occur repeatably at peak amplitudes of motion during walking. Similarly, peak angular movements may lead to additional axial strains of up to 25% at the external cortex and shear movements may lead to shear strains of up to 100%. Such strains are great enough to yield and possibly refracture the intra gap fracture tissue that may be composed of a combination of granulation tissue, fibrous cartilage, cartilage and bone. It was also shown that the procedure of releasing the fixator column to telescope (dynamize) has little influence on peak cyclic axial motion and on loading at the fracture, although increases occurred in peak transverse and torsional shear strains of up to 100%. Since permanent inter fragmentary translation also arises from the consequent compaction of the intra gap tissue, it may be permanent displacement rather than any change in the amplitude of motion that is responsible for the beneficial effect on healing claimed for the dynamizing procedure. In unstable fractures that are unable to support tibial load at the fracture, the peak amplitudes of cyclic movement were as great as those reported for fractures stabilized by plaster casts, and were approximately twice the movement of the stable fractures simulating early healing. Therefore, patients with unstable fractures supported by external fixators, may be expected to have similar patterns of healing to plaster-casted patients with similar fractures.
Optical identification using imperfections in 2D materials (2017)

The ability to uniquely identify an object or device is important for authentication. Imperfections, locked into structures during fabrication, can be used to provide a fingerprint that is challenging to reproduce. In this paper, we propose a simple optical technique to read unique information from nanometer-scale defects in 2D materials. Imperfections created during crystal growth or fabrication lead to spatial variations in the bandgap of 2D materials that can be characterized through photoluminescence measurements. We show a simple setup involving an angle-adjustable transmission filter, simple optics and a CCD camera can capture spatially-dependent photoluminescence to produce complex maps of unique information from 2D monolayers. Atomic force microscopy is used to verify the origin of the optical signature measured, demonstrating that it results from nanometer-scale imperfections. This solution to optical identification with 2D materials could be employed as a robust security measure to prevent counterfeiting.
The effect of elemental red phosphorus on the thermal degradation of poly(ethylene terephthalate) (1977)

The influence of elemental red phosphorus on the rate of thermal degradation of poly(ethylene terephthalate) has been studied in the temperature range of 400° to 500°C in inert and oxidizing atmospheres by means of isothermal thermogravimetric analysis. Addition of red phosphorus is found to reduce the rates of both the pyrolytic and the thermo-oxidative degradation and to increase the amount of residual char. A correlation of pyrolysis rates with oxygen index data has been attempted.