gebhart/patrepeval huggingface dataset:
Acyl Carrier Protein Is Conjugated to Glutathione in Spinach Seed (1991)

Acyl carrier protein (ACP) contains an essential sulfhydryl group in its phosphopantetheine prosthetic group. We have investigated the state of this sulfhydryl in developing and mature spinach seed (Spinacia oleracea). Seed extracts were separated on sodium dodecyl sulfate or native polyacrylamide gels, blotted to nitrocellulose, and probed with antibodies raised against spinach ACP-I. In extracts of mature seeds prepared with reducing agents, ACP-II migrated as a single major band, whereas extracts prepared without reducing agents gave two major bands. The additional band was identified as a conjugate of ACP-II to glutathione (ACP-S-S-G) on the basis of its sensitivity to reducing agents and its comigration with standards in both native and sodium dodecyl sulfate gel electrophoresis. In developing spinach seeds ACP-II exists primarily in its free sulfhydryl form or as acyl derivatives, with essentially no ACP-S-S-G present. During later stages of seed development, as seed water content declines, ACP-S-S-G accumulates to approximately 50% of the total ACP. Seed imbibition results in a rapid decline in ACP-S-S-G levels. The ACP-S-S-G:ACP-SH ratio of seeds during storage was found to be a function of seed water content and this could be manipulated by controlling the relative humidity under which the seeds were stored. We speculate that conjugation of ACP to glutathione protects the ACP from sulfhydryl oxidative damage in dry seeds.
Ship Survivability (part II) Ship Infrared (IR) signatures (1998)

A low lnfrared (lR) signature is of paramount importance for a warship's survivability, In this paper,
Asymmetric ATM-PON interface compliant to ITU-T/FSAN standard for global optical access systems (1999)

This paper presents a first optical access implementation prototype of asymmetric 622 Mbps/156 Mbps ATM-PON interface compliant to ITU-T/FSAN standard, with a dynamic bandwidth allocation mechanism for efficient IP traffic accommodation.
Noninvasive and cuffless measurements of blood pressure for telemedicine (2005)

Presents a new method for obtaining blood pressure readings noninvasively with telemedicine application. Through the pulse transit time technique, the systolic, diastolic, and mean blood pressures can be predicted using the time interval between the electrocardiogram (ECG) and photoplethysmography (PPG). The data can then be relayed to the Internet for analysis and viewing. The wireless application protocol (WAP) is used for displaying the information on portable wireless devices. The subjects' cardiovascular condition can thus be obtained for monitoring or pre-diagnosis purposes.
Nitric oxide reduces bacterial superantigen-immune cell activation and consequent epithelial abnormalities (2002)

Inhibition of the inducible form of nitric oxide (NO) synthase prolonged the murine enteropathy evoked by the bacterial superantigen, Staphylococcus aureus enterotoxin B (SEB). We examined the ability of NO to alleviate SEB-induced epithelial dysfunction and immune cell activation. Human peripheral blood mononuclear cells (PBMC) were activated by SEB for 24 h +/- the NO donors, S-nitroso-N-acetylpenicillamine and spermine-NONOate. The conditioned medium (CM) was collected and applied to T84 epithelial monolayers, and permeability [i.e., transepithelial resistance (TER)] and stimulated ion transport (i.e., short-circuit current responses to carbachol and forskolin) were assessed 24 h later. Exposure to CM led to an approximately 40% drop in TER and hyporesponsiveness to both secretagogues. CM made in the presence of NO donors (10(-4) M) had no significant effect on epithelial barrier or ion transport parameters. NO donors alone had no effect on naive epithelia, and addition of the NO donors to previously made CM did not affect the ability of this CM to alter epithelial function. Moreover, the NO donors dose-dependently reduced SEB-evoked PBMC proliferation and cytokine production (i.e., interferon-gamma, tumor necrosis factor alpha) but did not affect viability. These findings suggest a beneficial role for NO in inflammation by reducing immune cell activation and thus ameliorating consequent physiological abnormalities, in this instance, perturbed epithelial permeability and active ion transport.
Non-Lethal Endotoxin Injection: A Rat Model of Hypercoagulability (2017)

Systemic inflammation co-activates coagulation, which unchecked culminates in a lethal syndrome of multi-organ microvascular thrombosis known as disseminated intravascular coagulation (DIC). We studied an endotoxin-induced inflammatory state in rats to identify biomarkers of hemostatic imbalance favoring hypercoagulability. Intraperitoneal injection of LPS at 15 mg/kg body weight resulted in peripheral leukopenia and widespread neutrophilic sequestration characteristic of an acute systemic inflammatory response. Early indicators of hemostatic pathway activation developed within 4 hours, including increased circulating concentrations of procoagulant extracellular vesicles (EVs), EVs expressing endothelial cell and platelet membrane markers, and high concentration of soluble intercellular adhesion molecule-1 (sICAM-1), plasminogen activator inhibitor-1 (PAI-1), and D-dimers. Inflammation persisted throughout the 48-hour observation period; however, increases were found in a subset of serum microRNA (miRNA) that coincided with gradual resolution of hemostatic protein abnormalities and reduction in EV counts. Dose-adjusted LPS treatment in rats provides a time-course model to develop biomarker profiles reflecting procoagulant imbalance and rebalance under inflammatory conditions.
A New Chiral Rh(II) Catalyst for Enantioselective [2 + 1]-Cycloaddition. Mechanistic Implications and Applications (2004)

A novel chiral Rh(II) catalyst (1) is introduced for the [2 + 1]-cycloaddition of ethyl diazoacetate to terminal acetylenes and olefins with high enantioselectivity. The catalyst 1 consists of one acetate bridging group and three mono-N-triflyldiphenylimidazoline-2-one bidentate ligands (DPTI) spanning the Rh(II)-Rh(II) metallic center in a structure that was determined by single-crystal X-ray diffraction analysis. A rational mechanism is advanced that provides a straightforward explanation for the enantioselectivity and absolute stereochemical course of the [2 + 1]-cycloaddition reactions. A key element in this explanation is the cleavage of one of the Rh-O bonds of the bridging acetate group in the intermediate Rh-carbene complex to form a new pentacoordinate Rh carbene complex (formally 1.5 valent Rh) that can undergo [2 + 2]-cycloaddition with the C-C pi-bond of the acetylenic or olefinic substrate. Reductive elimination of the resulting adduct affords the cyclopropene or cyclopropane product. The C2-symmetry of the two DPTI ligands orthogonal to the bridging acetate also contributes to the high observed enantioselectivity and mechanistic clarity. The catalyst 1, which functions effectively at 0.5 mol %, can be recovered efficiently for reuse. Its ready availability, robustness, and effectiveness suggest it as a useful addition to the list of practical chiral Rh(II) catalysts for synthesis.
Novel Route to Synthesize Nanosized β-Zeolite with High Surface Area (2007)

Abstract Nanosized β-zeolite was synthesized in presence of two templates viz., tetraethylammonium hydroxide and cetyltrimethylammonium bromide by steaming dry-gel precursors.
Intelligent FDI management in the adaptive tactical navigator (2003)

The authors examine the use of a failure detection and identification (FDI) method in the adaptive tactical navigator (ATN) as a means for monitoring the health of navigation-aiding equipment. The objective of FDI is to detect sensor failures before the problem worsens to the point of requiring pilot intervention. The method uses parity functions to compare dissimilar measurements, and thus utilize the inherent analytical redundancy of measurements in multisensor navigation systems. Preliminary simulation results are presented to demonstrate the workings of the FDI method. It is concluded that the use of sets of parity functions, derived from different sensor combinations, holds promise as a device for the identification of sensor failures. The simulation example suggests that the method can detect and identify simple failures in a reasonably short time (under a minute).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Altered heart rate and blood pressure variability in mice lacking the Mas protooncogene (2000)

Heart rate variability is a relevant predictor of cardiovascular risk in humans. A significant genetic influence on heart rate variability is suggested, although the genes involved are ill-defined. The Mas-protooncogene encodes a G-protein-coupled receptor with seven transmembrane domains highly expressed in testis and brain. Since this receptor is supposed to interact with the signaling of angiotensin II, which is an important regulator of cardiovascular homeostasis, heart rate and blood pressure were analyzed in Mas-deficient mice. Using a femoral catheter the blood pressure of mice was measured for a period of 30 min and 250 data values per second were recorded. The mean values and range of heart rate and blood pressure were then calculated. Neither heart rate nor blood pressure were significantly different between knockout mice and controls. However, high resolution recording of these parameters and analysis of the data by non-linear dynamics revealed significant alterations in cardiovascular variability in Mas-deficient animals. In particular, females showed a strong reduction of heart rate variability. Furthermore, the data showed an increased sympathetic tone in knockout animals of both genders. The marked alterations detected in Mas-deficient mice of both genders suggest that the Mas-protooncogene is an important determinant of heart rate and blood pressure variability.
Study on integration technologies of building automation systems based on web services (2009)

At present, there are three kinds of difficult integration problems in building automation systems. One is the integration between building automation systems (BAS) and existing enterprise applications. The second is the integration of building automation systems which adopt different international standardized protocols (e.g. BACnet or LonTalk). The third is the integration of building automation subsystems (e.g. HVAC control, lighting control or fire alarming). The ldquointelligencerdquo of intelligent buildings is still more of a promise than a reality. Web Services, defined as self-contained, modular software components that can be described, published, located and invoked over the Internet using existing IT standards creates a new wave of evolution and pushes BAS industry into a new paradigm. Web Services provide new solutions to overcome above problems. The paper analyses the development procedures and current problems of the BAS industry, proposes that Web Services based on XML, SOAP, WSDL and UDDI are the best answer in solving the integration problems of building automation systems. The paper also describes integration technologies, framework model and realization principles of BAS based on Web Services.
The Use of Qualitative Content Analysis in Case Study Research (2008)

This paper aims at exploring and discussing the possibilities of applying qualitative content analysis as a (text) interpretation method in case study research. First, case study research as a research strategy within qualitative social research is briefly presented. Then, a basic introduction to (qualitative) content analysis as an interpretation method for qualitative interviews and other data material is given. Finally the use of qualitative content analysis for developing case studies is examined and evaluated. The author argues in favor of both case study research as a research strategy and qualitative content analysis as a method of examination of data material and seeks to encourage the integration of qualitative content analysis into the data analysis in case study research. URN: urn:nbn:de:0114-fqs0601211
Effect of sulphur concentration on bioleaching of heavy metals from contaminated dredged sediments (2009)

The sulphur-based bioleaching process using sulphur-oxidizing bacteria (SOB) has been demonstrated to be a feasible technology for removing heavy metals from contaminated sediments, but the excess sulphur application will lead to the re-acidification of bioleached sediments. The objective of the present study was to examine the effect of sulphur concentration on the bioleaching of heavy metals from contaminated sediments, with the ultimate purpose of minimizing the sulphur addition. The results showed that the inoculation of 7% of indigenous SOB, containing 3.6 x 10(8) colony forming units (CFU) mL(-1), and addition of elemental sulphur as a substrate (0.5 to 7.0 g L(-1)) resulted in a sharp decrease in sediment pH from an initial pH 8.0 to pH 1.4-2.4 and an increase in ORP (oxidation-reduction potential) from -10 mV to 500 mV within 10 days of bioleaching. Although the increase in sulphur concentration enhanced the rates of pH reduction and ORP elevation, the bioleaching process with the addition of 3.0 g L(-1) of sulphur was already sufficient to reach conditions of acidity (pH < 2.0) and ORP (500 mV) necessary for a satisfactory removal of metals, and, at day 10, 71.8% of Cu, 58.2% of Zn, and 25.3% of Cr were removed from the sediments. During the bioleaching process, Zn removal increased with a reduction in pH, whereas the removal of Cu and Cr increased not only with a reduction in pH but also with an increase in ORP. Results of sequential selective extraction indicated that the final levels of metal removals were dependent on their speciation distribution in the original sediments, and after bioleaching those unremoved metals in the bioleached sediments mainly existed in the residual fraction.
The impact of Lys→Arg surface mutations on the crystallization of the globular domain of RhoGDI (2004)

The potential of rational surface mutagenesis for enhanced protein crystallization is being probed in an ongoing effort. In previous work, it was hypothesized that residues with high conformational entropy such as Glu and Lys are suitable targets for surface mutagenesis, as they are rarely incorporated in crystal contacts or protein-protein interfaces. Previous experiments using Lys-->Ala, Glu-->Ala and Glu-->Asp mutants confirmed that mutated proteins were more likely to crystallize. In the present paper, the usefulness of Lys-->Arg mutations is studied. Several mutations of the globular domain of human RhoGDI were generated, including the single mutants K105R, K113R, K127R, K138R and K141R, the double mutants K(98,99)R and K(199,200)R and the triple mutants K(98,99,105)R and K(135,138,141)R. It is shown that Lys-->Arg mutants are more likely to crystallize than the wild-type protein, although not as likely as Lys-->Ala mutants. Out of the nine mutants tested, five produced diffracting crystals, including the K(199,200)R double mutant, which crystallized in a new space group and exceeded by approximately 1.0 A the resolution of the diffraction of the wild-type crystal. Major crystal contacts in the new lattice were created by the mutated epitope.
Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTrimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassaysMartin A. Hamilton, Rosemarie C. Russo, and Robert V. ThurstonCite this: Environ. Sci. Technol. 1977, 11, 7, 714–719Publication Date (Print):July 1, 1977Publication History Published online1 May 2002Published inissue 1 July 1977https://pubs.acs.org/doi/10.1021/es60130a004https://doi.org/10.1021/es60130a004research-articleACS PublicationsRequest reuse permissionsArticle Views5420Altmetric-Citations1851LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Survey on Joint Compression and Encryption Techniques for Video Data (2012)

Problem statement: When we send any video data over the network it consumes more time. This is due to the huge size of the video file when compared to text file. Therefore, video data should be compressed before sending to the destination. Another important factor during data transfer is security. Joint compression and encryption is employed to enable faster and secured transmission of video data. Approach: Compression and encryption algorithms can be classified into two main categories: Independent encryption technique and joint compression and encryption technique. Independent encryption techniques can further be classified as heavy weight and light weight encryption algorithms. There are many algorithms available in the joint compression and encryption technique. Comparative study of the above mentioned algorithms is done in this study. Results: Based on our study, found joint compression and encryption algorithms reduced 40% of the memory storage size and they increased execution speed up to 21%. Conclusion: Joint compression and encryption algorithms perform better in terms of speed and security when compared to independent encryption algorithms. This is because they employ compression before encryption.
Cell-Derived Matrix Enhances Osteogenic Properties of Hydroxyapatite (2010)

The study aimed to evaluate osteogenic properties of hydroxyapatite (HA) scaffold combined with extracellular matrix (ECM) derived in vitro from rat primary calvarial osteoblasts or dermal fibroblasts. The cellular viability, and the ECM deposited onto synthetic HA microparticles were assessed by MTT, Glycosaminoglycan, and Hydroxyproline assays as well as immunohistochemistry and scanning electron microscopy after 21 days of culture. The decellularized HA-ECM constructs were implanted in critical-sized calvarial defects of Sprague-Dawley rats, followed by bone repair and local inflammatory response assessments by histomorphometry and immunohistochemistry at 12 weeks postoperatively. We demonstrated that HA supported cellular adhesion, growth, and ECM production in vitro, and the HA-ECM constructs significantly enhanced calvarial bone repair (p<0.05, Mann-Whitney U-test), compared with HA alone, despite the significantly increased number of CD68+ macrophages, and foreign body giant cells (p<0.05, Mann-Whitney U-test). Selective accumulation of bone sialoprotein, osteopontin, and periostin was observed at the tissue-HA interfaces. In conclusion, in vitro-derived ECM mimics the native bone matrix, enhances the osteogenic properties of the HA microparticles, and might modulate the local inflammatory response in a bone repair-favorable way. Our findings highlight the ability to produce functional HA-ECM constructs for bone tissue engineering applications.
Recent progress in the development of plant derived vaccines (2008)

Recombinant subunit vaccines have been with us for the last 30 years and they provide us with the unique opportunity to choose from the many available production systems that can be used for recombinant protein expression. Plants have become an attractive production platform for recombinant biopharmaceuticals and vaccines have been at the forefront of this new and expanding industry sector. The particular advantages of plant-based vaccines in terms of cost, safety and scalability are discussed in the light of recent successful clinical trials and the likely impact of plant systems on the vaccine industry is evaluated.
Treatment of Cancer Pain with Epidural Butyl-Amino-Benzoate Suspension (1987)

Seven patients with intractable cancer pain received a total of ten epidural and two peripheral nerve blocks with a 10% aqueous suspension of n-butyl-p-amino-benzoate. All patients achieved good to complete pain relief. The daily requirement of narcotics was greatly reduced in all patients and entirely eliminated in some. Pain relief persisted until death in all patients except one where pain returned after 8 months and was subsequently eliminated with a repeat block. There were no complications.
Identification and Expression of the <i>Bacillus subtilis</i> Fructose-1,6-Bisphosphatase Gene ( <i>fbp</i> ) (1998)

The Bacillus subtilis fbp gene encoding fructose-1,6-bisphosphatase (FBPase) was originally identified as yydE. The fbp gene was expressed at a fairly constant level in cells undergoing glycolysis or gluconeogenesis. fbp transcription was initiated 94 bp upstream of the translation initiation codon, resulting in a 2.4-kb monocistronic transcript. Interestingly, B. subtilis FBPase exhibited no significant similarity to other FBPases in protein sequence databases.
Determination of Particle Size Distributions by Laser Diffraction (2012)

The paper deals with the main principles of determination of particle size distribution using Mastersizer 2000, Malvern Instruments Ltd., UK. On the example of several problems we have demonstrated that the method is not a routine one and that the measurement procedure is not limited to entering a sample into the dispersion unit and pressing the button. Furthermore, we have shown that the sample preparation method and, therefore, the accuracy of results conclusively depend on physical and chemical properties of the analyzed materials.
Study on Driver Emotion in Driver-Vehicle-Environment Systems Using Multiple Networked Driving Simulators (2007)

Driving simulators have been widely used to investigate driver behavior and psychophysiological responses. In this paper, the authors proposed to use multiple participant-operated driving simulators which communicate with each other so the platoon formed by the simulators can demonstrate realistic effects of driver-driver interaction in simulation scenarios. In our preliminary experiment, three driving simulators were connected through Northeastern’s campus network. One was a fixed-based high fidelity driving simulator with an actual automobile cabin and large curve screen in our Virtual Environments Laboratory. The other two are relative low cost simulators housed in the Intelligent Human-Machine Systems Laboratory. With this configuration, we investigated emotional behavior (anger, neutral, and excitation) of drivers by collecting driving performance data, psychophysiological responses and eye movement data. The results demonstrated the feasibility and efficiency of using multiple networked driving simulators to study driver emotional behavior, e.g., road rage.