gebhart/patrepeval huggingface dataset:
Discrimination among Protein Variants Using an Unfoldase-Coupled Nanopore (2014)

Previously we showed that the protein unfoldase ClpX could facilitate translocation of individual proteins through the α-hemolysin nanopore. This results in ionic current fluctuations that correlate with unfolding and passage of intact protein strands through the pore lumen. It is plausible that this technology could be used to identify protein domains and structural modifications at the single-molecule level that arise from subtle changes in primary amino acid sequence (e.g., point mutations). As a test, we engineered proteins bearing well-characterized domains connected in series along an ∼700 amino acid strand. Point mutations in a titin immunoglobulin domain (titin I27) and point mutations, proteolytic cleavage, and rearrangement of beta-strands in green fluorescent protein (GFP), caused ionic current pattern changes for single strands predicted by bulk phase and force spectroscopy experiments. Among these variants, individual proteins could be classified at 86-99% accuracy using standard machine learning tools. We conclude that a ClpXP-nanopore device can discriminate among distinct protein domains, and that sequence-dependent variations within those domains are detectable.
Alginate as a new biomaterial for the growth of porcine retinal pigment epithelium (2003)

Porcine RPE cells can be propagated in alginate culture with a significant increase in cell numbers while maintaining normal morphology. Under the conditions described in the present study, the dedifferentiation of porcine RPE induced by standard in vitro culture methods is reversible.
Sol-Gel Silica: Properties, Processing and Technology Transfer (1998)

The Concept * Sol-Gel Kinetics * Aging * Drying * Stabilization * Densification * Laser Densification * Gel-Silica Optics * Porous Gel-Silica Optical Matrices * Biological Implications * Technology Transfer of Gel-Silica Optics.
A 25/spl mu/W 100kS/s 12b ADC for wireless micro-sensor applications (2006)

A 0.18μm CMOS 12b 100kS/s successive approximation ADC is presented. The entire ADC consumes 25μW from a 1V supply and achieves an SNDR of 65dB. Its sampling rate can be scaled, yielding linear power savings. Efficiency of the comparator is increased by an offset compensating latch, while noise performance and common-mode rejection are improved by a modified capacitor network
Enhanced Calibration of Robot Tool Centre Point Using Analytical Algorithm (2014)

This paper introduces an enhanced method to analytically identify an unknown tool centre point (TCP) of standard industrial robots with six degrees of freedom. The method uses a mathematical closed form to determine the position and orientation (pose) of a mounted robot tool frame in relation to the robot flange frame. This unknown pose is represented by a homogeneous transformation that is calculated by performing two robot movements. The position and orientation of the robot is measured after each movement, which leads to an equation of the form , where represents the unknown homogeneous transformation from the robot flange to the TCP. The achievable accuracy in comparison to existing methods is enhanced by using relative movements with orthogonal rotational axes. This ensures optimal error propagation for solving the resulting equation and leads to a very accurate calculation of the unknown transformation. The described method was evaluated and tested on a standard industrial robot. All measurements to determine the position and orientation of the robot tool were done with a laser tracker. A typical application example from the field of automated machining demonstrates the use of the developed method.
Is the gluten free diet the best choice for symptom relief in people who don't have coeliac disease? (2014)

Many people must follow the gluten free diet for solid medical reasons, including coeliac disease or dermatitis herpetiformis. However, it has been estimated that for every person diagnosed with coeliac disease in Australia, twenty people without coeliac disease follow a gluten free diet.
Piaget and egocentrism: A perspective on recent developmental research (1983)

(1983). Piaget and egocentrism: A perspective on recent developmental research. Early Child Development and Care: Vol. 12, No. 1, pp. 7-18.
Face Recognition in Video Using a What-and-Where Fusion Neural Network (2007)

A what-and-where fusion neural network is applied to the recognition of human faces from video sequences. The spatio-temporal information contained in successive video frames allows to effectively accumulate a classifier's predictions for each person being tracked in an environment. In a particular realization of this network, a fuzzy ARTMAP neural network is used to classify faces detected in each frame, while a bank of Kalman filters is used to track blobs that contain the extracted faces moving in the environment. Performance of the what-and-where fusion neural network is compared to that of the fuzzy ARTMAP and k-nearest-neighbor (k-NN) classifiers in terms of classification rate, convergence time and compression. In this paper, the impact on performance of setting different region of interest (ROI), of optimizing fuzzy ARTMAP parameters, and of selecting different training subset sizes, is assessed. Simulation results on real-world video sequences indicate that this network can achieve a classification rate that is significantly higher (by approximately 50% in some cases) than that of fuzzy ARTMAP alone, and than that of the k-NN.
Environmental Science (2017)

Environmental science is a wide field of research that examines all aspects of how environments develop, change, and are impacted by human activity. This includes work on the basic principles of environmental processes and change, but also applied research into policy‐relevant issues, such as climate change and pollution, as well as human–environment research into how human activity impacts on the environment and how this impact might be ameliorated. Environmental science has developed alongside the environmental movement in recent decades and, like all scientific work, it is shaped by this sociopolitical context. Drawing on different disciplines such as geology, ecology, chemistry, geography, and meteorology, it is sometimes considered multidisciplinary or interdisciplinary, although some argue it must become transdisciplinary to fully tackle the challenges of sustainability.
Sequential Infiltration Synthesis for the Design of Low Refractive Index Surface Coatings with Controllable Thickness (2017)

Control over refractive index and thickness of surface coatings is central to the design of low refraction films used in applications ranging from optical computing to antireflective coatings. Here, we introduce gas-phase sequential infiltration synthesis (SIS) as a robust, powerful, and efficient approach to deposit conformal coatings with very low refractive indices. We demonstrate that the refractive indices of inorganic coatings can be efficiently tuned by the number of cycles used in the SIS process, composition, and selective swelling of the of the polymer template. We show that the refractive index of Al<sub>2</sub>O<sub>3</sub> can be lowered from 1.76 down to 1.1 using this method. The thickness of the Al<sub>2</sub>O<sub>3</sub> coating can be efficiently controlled by the swelling of the block copolymer template in ethanol at elevated temperature, thereby enabling deposition of both single-layer and graded-index broadband antireflective coatings. Using this technique, Fresnel reflections of glass can be reduced to as low as 0.1% under normal illumination over a broad spectral range.
Deep learning predicts function of live retinal pigment epithelium from quantitative microscopy (2019)

Increases in the number of cell therapies in the preclinical and clinical phases have prompted the need for reliable and noninvasive assays to validate transplant function in clinical biomanufacturing. We developed a robust characterization methodology composed of quantitative bright-field absorbance microscopy (QBAM) and deep neural networks (DNNs) to noninvasively predict tissue function and cellular donor identity. The methodology was validated using clinical-grade induced pluripotent stem cell-derived retinal pigment epithelial cells (iPSC-RPE). QBAM images of iPSC-RPE were used to train DNNs that predicted iPSC-RPE monolayer transepithelial resistance, predicted polarized vascular endothelial growth factor (VEGF) secretion, and matched iPSC-RPE monolayers to the stem cell donors. DNN predictions were supplemented with traditional machine-learning algorithms that identified shape and texture features of single cells that were used to predict tissue function and iPSC donor identity. These results demonstrate noninvasive cell therapy characterization can be achieved with QBAM and machine learning.
Potentiation of the antiviral activity of poly r(A-U) by xanthene dyes (1990)

Ten xanthene dyes (XAN) are evaluated for their ability to potentiate the antiviral activity of poly r(A-U) using a human foreskin fibroblast-vesicular stomatitis virus bioassay in which the XAN is combined with 0.2 mM poly r(A-U) to produce a XAN/ribonucleotide ratio of 1/4. Four of the ten XANs tested in this study, rhodamine 123, rhodamine B, rhodamine 6G and sulforhodamine B, enhance the antiviral activity of poly r(A-U) 8- to 15-fold. The interferon-inducing activity of the four active XAN/poly r(A-U) combinations is equal to the sum of the activities of their constituents. These four XANs appear to potentiate the antiviral activity of the poly r(A-U) without superinduction of interferon. The direct viral inactivation study demonstrates that the XANs, poly r(A-U) and the XAN/poly r(A-U) combinations do not inactivate the VSV at concentrations near the 50% effective dose.
Levosimendan in pulmonary hypertension and right heart failure (2018)

Pulmonary hypertension is a multifactorial disease with a high morbidity and mortality. Right ventricular function is the most important predictor of morbidity and mortality in patients suffering from pulmonary hypertension, but currently there are no approved treatments directly supporting the failing right ventricle. Levosimendan is a calcium sensitizing agent with inotropic, pulmonary vasodilatory, and cardioprotective properties. Given its pharmacodynamic profile, levosimendan could be a potential novel agent for the treatment of right ventricular failure caused by pulmonary hypertension. The aim of this review is to provide an overview of the current knowledge on the effects of levosimendan in pulmonary hypertension and right heart failure.
Polystyrene-Supported Hydroxyproline:  An Insoluble, Recyclable Organocatalyst for the Asymmetric Aldol Reaction in Water (2006)

4-Hhydroxyproline has been anchored to a polystyrene resin through click chemistry, and the resulting catalyst has been successfully applied to the direct aldol reaction in water. The high hydrophobicity of the resin and the presence of water are key to ensuring high stereoselectivity, whereas yield can be increased by using catalytic amounts of DiMePEG. This effect has been further demonstrated by the inefficiency of a homogeneous, more polar analogue.
The roles of phase separation mechanism and coarsening in the formation of poly(methyl methacrylate) asymmetric membranes (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe roles of phase separation mechanism and coarsening in the formation of poly(methyl methacrylate) asymmetric membranesFu Jya Tsai and John M. TorkelsonCite this: Macromolecules 1990, 23, 3, 775–784Publication Date (Print):February 1, 1990Publication History Published online1 May 2002Published inissue 1 February 1990https://pubs.acs.org/doi/10.1021/ma00205a014https://doi.org/10.1021/ma00205a014research-articleACS PublicationsRequest reuse permissionsArticle Views977Altmetric-Citations132LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Enhanced Electrical Switching and Electrochromic Properties of Poly(p‐phenylenebenzobisthiazole) Thin Films Embedded with Nano‐WO<sub>3</sub> (2010)

Abstract The electrical switching and electrochromic phenomena of a novel nanocomposite comprising poly(p‐phenylenebenzobisthiazole) (PBZT) and tungsten oxide (WO 3 ) nanoparticles are investigated as a function of the nanoparticle loading. Both dissolving PBZT and doping PBZT backbone structure with acid are achieved by one simple step. Chlorosulfonic acid (CSA) is used as a solvent and spontaneously transformed to sulfuric acid upon exposure to moisture. The formed sulfuric acid serves as doping agent to improve the electrical conductivity of PBZT. The most significant enhancement of electrical switching is observed in the nanocomposites with low weight fraction (5%). The electrical conductivity of 5% WO 3 /PBZT nanocomposite thin film is increased by about 200 times and 2 times, respectively, as compared to those of the as‐received PBZT and PBZT/CSA thin films. As the nanoparticle loading increases to 20% and 30%, the nanocomposites follow an ohmic conduction mechanism. Stable electrical conductivity switching is observed before and after applying a bias on the pristine PBZT and WO 3 /PBZT nanocomposite thin films. Electrochromic phenomena of both PBZT and WO 3 /PBZT nanocomposite thin films with high contrast ratio are observed after applying a bias (3 V). The mechanisms of the nanoparticles in enhancing the electrical switching and electrochromic properties are proposed.
Women in Combat (2013)

The US secretary of defense announced in January 2013 that, from 2016, women will be allowed to serve in ground-combat roles in the US armed forces. The UK is likely to soon be faced with the need to make a similarly historic decision, having answered key questions such as what is actually known so far about the integration of women into combat units, and its potential impact on cohesion. Anthony King provides a fascinating comparison of the experiences of several Western armed forces, and concludes that in today's world of professional armies, it is not gender that determines cohesion, but training and competence.
Evaluation of earliness of wheat by dividing the growth duration into four developmental periods. (1985)

わが国コムギ品種・系統がもっている早熟性の特性を再評価することによって，早熟化の可能性を見出そうとした.すなわち，コムギの全生育期間を播種～茎立，茎立～出穂，出穂～開花，開花～成熟期の4つの期間に分割して，個々の期問の短い品種・系統の遺伝特性を育種的に集積することにより，早熟化の可能性を検討した.コムギ農林登録品種とその系譜上の計296品種・系統について，茎立，出穂，開花，成熟期を2ケ年圃場調査した.同一出穂期および同一成熟期の品種問で，茎立期および開花期に早晩があり，その結果，同じ生育環境下で4つの生育期間に品種間差異があることを認めた.茎立期あるいは出穂期とその後の各生育期間との間には，高い負の相関関係が認められた.しかし，この関係からはずれた各生育期間の短い有用育種素材を選定することができた.これらの育種素材の組合せにより，生育期間の短い遺伝特性が組換えられたと仮定しても，農林61号より1週間程度早熟な実用品種の育成が限界であるとみられた.さらに早熟化のための育種方法を考察した.
Rate Constant for Long-Chain Branch Formation in Free-Radical Polymerization of Ethylene (1979)

Abstract Quantitative measurements on the rate constant for long-chain branching in radical polymerization of ethylene have not yet been cited. This paper presents a convenient method of calculating the ratio of the rate constant for long-chain branching to that for propagation, by means of polymerization parameters, average degrees of polymerization (Xn and Xw), and amount of total unsaturation. The results of calculation based on our experimental data show that the activation energy Etrp -Ep, and the activation volume ΔV trp ∗-ΔV ∗p were 3.6 kcal/mole and 24 cc/mole, respectively, in radical polymerization of ethylene. The validity of these values is discussed.
Universal Chroma Subsampling Strategy for Compressing Mosaic Video Sequences With Arbitrary RGB Color Filter Arrays in H.264/AVC (2012)

In this paper, we propose a universal chroma subsampling strategy for compressing mosaic video sequences with arbitrary red-green-blue (RGB) color filter arrays (CFAs), which are widely used in the single sensor imaging pipeline, in H.264/AVC. We first develop a modified universal demosaicing scheme, which specifically recovers the G component in the color difference domain, to recover the missing color components in the input mosaic image frames. Then, based on the transform between the RGB and the YUV color spaces, the proposed universal subsampling strategy automatically samples, by considering the significance of the U and V components for reconstructing R and B pixels, the proper U and V chroma components according to the corresponding mosaic structure. To the best of our knowledge, this is the first universal chroma subsampling strategy designed specifically for mosaic video sequences with arbitrary RGB-CFAs. Experimental results on mosaic video sequences with seven common types of the RGB-CFAs demonstrate that the proposed universal chroma subsampling strategy is superior to the conventional strategy of H.264/AVC. Moreover, integrating the proposed universal demosaicing scheme and the chroma subsampling strategy can deliver better video sequence quality.
Graph Transduction as a Noncooperative Game (2011)

Graph transduction is a popular class of semisupervised learning techniques that aims to estimate a classification function defined over a graph of labeled and unlabeled data points. The general idea is to propagate the provided label information to unlabeled nodes in a consistent way. In contrast to the traditional view, in which the process of label propagation is defined as a graph Laplacian regularization, this article proposes a radically different perspective, based on game-theoretic notions. Within the proposed framework, the transduction problem is formulated in terms of a noncooperative multiplayer game whereby equilibria correspond to consistent labelings of the data. An attractive feature of this formulation is that it is inherently a multiclass approach and imposes no constraint whatsoever on the structure of the pairwise similarity matrix, being able to naturally deal with asymmetric and negative similarities alike. Experiments on a number of real-world problems demonstrate that the proposed approach performs well compared with state-of-the-art algorithms, and it can deal effectively with various types of similarity relations.
A theoretical calculation of the electric field induced by magnetic stimulation of a peripheral nerve (1990)

A mathematical model is presented that predicts the electric field induced in the arm during magnetic stimulation of a peripheral nerve. The arm is represented as a homogeneous, cylindrical volume conductor. The electric field arises from two sources: the time-varying magnetic field and the accumulation of charge on the arm surface. In magnetic stimulation both of these contributions are significant. The magnitude of the electric field is greatest near the surface of the arm, and is well localized. Various coil orientations are examined; the smallest electric fields are induced when the coil is perpendicular to the arm surface, the largest when the coil is parallel. These results are consistent with many experimental observations in the literature, and aid in the basic understanding of magnetic stimulation of the peripheral nervous system.
Design of a Multi-Bunch BPM for the Next Linear Collider (2002)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Andrew Young, Douglas McCormick, Marc Ross, Stephen R. Smith, H. Hayano, T. Naito, N. Terunuma, S. Araki; Design of a Multi‐Bunch BPM for the Next Linear Collider. AIP Conf. Proc. 18 December 2002; 648 (1): 508–515. https://doi.org/10.1063/1.1524439 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Production of human suppressor T cell hybridomas. (1982)

To study human T cell suppression of immunoglobulin (Ig) synthesis with homogeneous populations of immunoregulatory cells, human suppressor T cell hybridomas were prepared by somatic cell fusion of concanavalin A-activated peripheral blood T cells with hypoxanthine-guanine phosphoribosyltransferase-(HGPRT, EC 2.4.2.8) deficient human leukemic CEM T cells. After selection in hypoxanthine-aminopterin-thymidine (HAT) medium and cloning by limiting cell dilution, two human T cell hybridomas were identified that produced 60 to 80% suppression of in vitro polyclonal immunoglobulin production when cocultured with pokeweed mitogen- (PWM) stimulated peripheral blood lymphocytes. Further, one of the suppressor T cell hybridomas constitutively secreted a soluble suppressor factor(s) (TsF) of m.w. 70,000 to 85,000 daltons, which produced reversible noncytotoxic inhibition of lectin-activated B cell Ig production. In contrast, this TsF did not inhibit lectin- or antigen-induced T cell proliferation, nor did it interfere with the generation or effector function of cytotoxic T cells. Additional studies indicated that this Tsf acts directly on B cells or monocytes rather than indirectly modulating the activity of immunoregulatory T cells. In summary, these studies suggest that techniques of somatic cell fusion may provide a valuable approach to further study human immunoregulatory cell-cell interactions as well as provide a source of sufficient quantities of important lymphokines for further purification and characterization.
A Role for CD81 in Early T Cell Development (1996)

Early stages of T cell development are thought to include a series of coordinated interactions between thymocytes and other cells of the thymus. A monoclonal antibody specific for mouse CD81 was identified that blocked the appearance of alpha beta but not gamma delta T cells in fetal organ cultures initiated with day 14.5 thymus lobes. In reaggregation cultures with CD81-transfected fibroblasts, CD4-CD8- thymocytes differentiated into CD4+CD8+ T cells. Thus, interactions between immature thymocytes and stromal cells expressing CD81 are required and may be sufficient to induce early events associated with T cell development.