gebhart/patrepeval huggingface dataset:
Analysis of Damage Tolerance Pathways in <i>Saccharomyces cerevisiae</i>: a Requirement for Rev3 DNA Polymerase in Translesion Synthesis (1998)

The replication of double-stranded plasmids containing a single N-2-acetylaminofluorene (AAF) adduct located in a short, heteroduplex sequence was analyzed in Saccharomyces cerevisiae. The strains used were proficient or deficient for the activity of DNA polymerase zeta (REV3 and rev3delta, respectively) in a mismatch and nucleotide excision repair-defective background (msh2delta rad10delta). The plasmid design enabled the determination of the frequency with which translesion synthesis (TLS) and mechanisms avoiding the adduct by using the undamaged, complementary strand (damage avoidance mechanisms) are invoked to complete replication. To this end, a hybridization technique was implemented to probe plasmid DNA isolated from individual yeast transformants by using short, 32P-end-labeled oligonucleotides specific to each strand of the heteroduplex. In both the REV3 and rev3delta strains, the two strands of an unmodified heteroduplex plasmid were replicated in approximately 80% of the transformants, with the remaining 20% having possibly undergone prereplicative MSH2-independent mismatch repair. However, in the presence of the AAF adduct, TLS occurred in only 8% of the REV3 transformants, among which 97% was mostly error free and only 3% resulted in a mutation. All TLS observed in the REV3 strain was abolished in the rev3delta mutant, providing for the first time in vivo biochemical evidence of a requirement for the Rev3 protein in TLS.
Heuristic-driven Techniques for Test Case Selection (2002)

We propose an approach to testing that combines formal methods with practical criteria, close to the testing engineer's experience. It can be seen as a framework to evaluate and select test suites using formal methods, assisted by informal heuristics. We also introduce the formalism of enriched transition systems to store information obtained during the testing phase, and to adapt classical test generation techniques to take advantage of the possibilities of the new formalism.
A Role for CD5 in TCR-Mediated Signal Transduction and Thymocyte Selection (1995)

CD5 is a transmembrane protein that is expressed on the surface of T cells and a subset of B cells. The absence of CD5 rendered thymocytes hyperresponsive to stimulation through the T cell antigen receptor (TCR) in vitro. Selection of T cells expressing three distinct transgenic TCRs was also abnormal in CD5-deficient mice. These observations indicate that CD5 can influence the fate of developing thymocytes by acting as a negative regulator of TCR-mediated signal transduction.
Response to reflected-force feedback to fingers in teleoperations (1989)

Reflected-force feedback is an important aspect of teleoperations. The objective is to determine the ability of the human operator to respond to that force. Telerobotics operation is simulated by computer control of a motor-driven device with capabilities for programmable force feedback and force measurement. A computer-controlled motor drive is developed that provides forces against the fingers as well as (angular) position control. A load cell moves in a circular arc as it is pushed by a finger and measures reaction forces on the finger. The force exerted by the finger on the load cell and the angular position are digitized and recorded as a function of time by the computer. Flexure forces of the index, long and ring fingers of the human hand in opposition to the motor driven load cell are investigated. Results of the following experiments are presented: (1) Exertion of maximum finger force as a function of angle; (2) Exertion of target finger force against a computer controlled force; and (3) Test of the ability to move to a target force against a force that is a function of position. Averaged over ten individuals, the maximum force that could be exerted by the index or long finger is about 50 Newtons, while that of the ring finger is about 40 Newtons. From the tests of the ability of a subject to exert a target force, it was concluded that reflected-force feedback can be achieved with the direct kinesthetic perception of force without the use of tactile or visual clues.
The Zero-Beat Method of Frequency Discrimination (1942)

A method of frequency discrimination in which the frequency of balance is determined solely by the frequency of the controlling oscillator is explained. The method utilizes the phase turnover, which occurs at zero beat, between two beat sources when one of the beating signals is dephased by 90 degrees before it is applied to one of the beat detectors. A network is inserted in one of the beat sources which shifts its phase an additional 90 degrees and makes its output a direction function of the frequency. The beats are then amplified and supplied to a balanced rectifier, from which a direct voltage is available which changes polarity with the direction of frequency deviation.
OVEREXPRESSION OF DIFFERENT MEMBERS OF THE TYPE 1 GROWTH FACTOR RECEPTOR FAMILY AND THEIR ASSOCIATION WITH CELL PROLIFERATION IN PERIAMPULLARY CARCINOMA (1996)

The expression of epidermal growth factor receptor (EGFR), c-erbB-2, and c-erbB-3 was examined immunohistochemically in 57 cases of periampullary carcinoma. The percentage of Ki-67-positive cells was also examined in the same tissue, to determine the relationship between the expression of the members of the type I growth factor receptor family and cell proliferation. In carcinoma of the head of pancreas, the percentage of cases with overexpression of c-erbB-3 was significantly higher than with overexpression of c-erbB-2 and EGFR. In contrast, in lower bile duct carcinoma and carcinoma of the ampulla of Vater, the percentages of cases with overexpression of c-erbB-2 was greater than with overexpression of other growth factor receptors. A higher percentage of cases with overexpression of c-erbB-3 in pancreatic head carcinoma and overexpression of c-erbB-2 in carcinoma of the ampulla of Vater was found in Ki-67 antigen-positive cases. Moreover, the overexpression of c-erb-3 in pancreatic head carcinoma, c-erb-2 in ampulla of Vater carcinoma, and Ki-67 in both carcinomas was found to be associated with poor patient outcome. These results demonstrate that different members of the type I growth factor receptor family are overexpressed in different carcinomas of the periampullary region.
A Model of Pulsations in Communicating Hydrocephalus (2002)

The traditional theory of communicating hydrocephalus has implicated the bulk flow component of CSF motion; that is, hydrocephalus is generally understood as an imbalance between CSF formation and absorption. The theory that the cause of communicating hydrocephalus is malabsorption of CSF at the arachnoid villi is not substantiated by experimental evidence or by physical reasoning. Flow-sensitive MRI has shown that nearly all CSF motion is pulsatile, and there is substantial evidence that hyperdynamic choroid plexus pulsations are necessary and sufficient for ventricular dilation in communicating hydrocephalus. We have developed a model of intracranial pulsations based on the analogy between the pulsatile motion of electrons in an electrical circuit and the pulsatile motion of blood and CSF in the cranium. Increased impedance to the flow of CSF pulsations in the subarachnoid space redistributes the flow of pulsations into the ventricular CSF and into the capillary and venous circulation. The salient features of communicating hydrocephalus, such as ventricular dilation, intracranial pressure waves, narrowing of the CSF-venous pressure gradient, diminished cerebral blood flow, elevated resistive index and malabsorption of CSF, emerge naturally from the model. We propose that communicating hydrocephalus is the result of a redistribution of CSF pulsations in the cranium.
Decreased toxicity of polymorphonuclear neutrophils toward hepatocytes isolated from rats with acute inflammatory reaction (1990)

We have recently demonstrated that polymorphonuclear neutrophils were toxic to hepatocytes through a protease-mediated mechanism. Since synthesis of antiproteases is markedly increased during acute inflammatory reaction, the aim of this work was to investigate the toxicity of neutrophils against normal vs. inflammatory rat hepatocytes. Acute inflammatory reaction was induced by subcutaneous injection of turpentine 24 hr before the experiments. Hepatocytes from normal and turpentine-treated rats were isolated by collagenase digestion. They were incubated with human neutrophils stimulated by 1 mg/ml opsonized zymosan. Cytotoxicity was quantified by the percentage of alanine aminotransferase activity released by hepatocytes in culture medium after an 18-hr incubation period. By comparison to normal hepatocytes, inflammatory hepatocytes were more resistant to the toxicity of neutrophils. At a neutrophil/hepatocyte ratio of 20:1, the alanine aminotransferase activity releases were 53.7% +/- 5.4% (mean +/- 1 S.E.) and 27.4% +/- 4.8% for normal and inflammatory hepatocytes, respectively. Similarly, inflammatory hepatocytes were found to be less sensitive than normal hepatocytes to the toxic effect of purified neutrophil cathepsin G. In contrast, both types of hepatocytes exhibited the same sensitivity to H2O2 generated by a system consisting of glucose and glucose oxidase. Two arguments suggested that the resistance of inflammatory hepatocytes to protease toxicity was explained by an increased production of antiproteases by these cells: (a) when tested against cathepsin G and porcine pancreatic elastase activities, the protease inhibitory capacity of conditioned medium from inflammatory hepatocytes was higher than that of conditioned medium from normal hepatocytes; (b) conditioned medium from inflammatory hepatocytes markedly reduced the toxicity of stimulated neutrophils as that of cathepsin G.(ABSTRACT TRUNCATED AT 250 WORDS)
Characterization of Adult Ghrelin and Ghrelin Receptor Knockout Mice under Positive and Negative Energy Balance (2007)

Ghrelin and the ghrelin receptor (GH secretagogue receptor, GHS-R), are believed to have important roles in energy homeostasis. We describe results from the first studies to be conducted in congenic (N10) adult ghrelin(-/-) and Ghsr(-/-) mice under conditions of both positive (high-fat diet) and negative (caloric restriction) energy balance. In contrast to results from young N2 mutant mice, changes in body weight and energy expenditure are not clearly distinguishable across genotypes. Although respiratory quotient was lower in mice fed a high-fat diet, no differences were evident between littermate wild-type and null genotypes. With normal chow, a modest decrease trend in respiratory quotient was detected in ghrelin(-/-) mice but not in Ghsr(-/-) mice. Under caloric restriction, the weight loss of ghrelin(-/-) and Ghsr(-/-) mice was identical to wild-type littermates, but blood glucose levels were significantly lower. We conclude that adult congenic ghrelin(-/-) and Ghsr(-/-) mice are not resistant to diet-induced obesity but under conditions of negative energy balance show impairment in maintaining glucose homeostasis. These results support our hypothesis that the primary metabolic function of ghrelin in adult mice is to modulate glucose sensing and insulin sensitivity, rather than directly regulate energy intake and energy expenditure.
Ammonia gas sensors based on poly (3-hexylthiophene)-molybdenum disulfide film transistors (2016)

In this work, in order to enhance the recovery performance of organic thin film transistors (OTFTs) ammonia (NH3) sensors, poly (3-hexylthiophene) (P3HT) and molybdenum disulfide (MoS2) were combined as sensitive materials. Different sensitive film structures as active layers of OTFTs, i.e., P3HT-MoS2 composite film, P3HT/MoS2 bilayer film and MoS2/P3HT bilayer film were fabricated by spray technology. OTFT gas sensors based on P3HT-MoS2 composite film showed a shorter recovery time than others when the ammonia concentration changed from 4 to 20 ppm. Specifically, x-ray diffraction (XRD), Raman and UV-visible absorption were employed to explore the interface properties between P3HT and single-layer MoS2. Through the complementary characterization, a mechanism based on charge transfer is proposed to explain the physical originality of these OTFT gas sensors: closer interlayer d-spacing and better π-π stacking of the P3HT chains in composite film have ensured a short recovery time of OTFT gas sensors. Moreover, sensing mechanisms of OTFTs were further studied by comparing the device performance in the presence of nitrogen or dry air as a carrier gas. This work not only strengthens the fundamental understanding of the sensing mechanism, but provides a promising approach to optimizing the OTFT gas sensors.
A Low-Voltage 30-GHz CMOS Divide-by-Three ILFD With Injection-Switched Cross-Coupled Pair Technique (2017)

This paper presents the design of a divide-by-three frequency divider operating at 30 GHz with an injection-switched cross-coupled pair topology. A wider locking range as well as a lower operation voltage can be achieved because of this newly proposed topology. The divider is implemented in a 90-nm standard CMOS process. The total locking range of the divider core is 4.5 GHz with a power consumption of 2.85 mW from a supply voltage of 0.5 V. The total power consumption of the buffers is 2.65 mW from a supply voltage of 1.0 V. The measured output phase noise is -141 dBc/Hz at 1-MHz offset when the input referred signal has a phase noise of -131 dBc/Hz at 1-MHz offset from 30 GHz. The phase-noise difference of 10 dB is close to the theoretical value of 9.5 dB for division-by-three. The total chip size is 0.48 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , and the divider core size is only about 0.14 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> .
Design of MEMS PZT Circular Diaphragm Actuators to Generate Large Deflections (2006)

This paper presents a design of lead zirconate titanate (PZT) circular diaphragm actuators to generate large deflections. The actuators utilize a unimorph structure consisting of an active PZT and a passive thermally grown SiO/sub 2/ layer. The diaphragm structures were formed by deep reactive ion etching (DRIE). Two different designs, where the PZT layer in the diaphragm actuators was driven by either interdigitated (IDT) electrodes or parallel plate electrodes, were investigated. Both finite element analysis and experimental results proved that the IDT configuration is favorable to generate deflections larger than the diaphragm thickness. The IDT configuration creates an expansion in the PZT layer in the radial direction and a contraction in the tangential direction under forward bias, which enables large deflections. At applied voltages of 100 V, an actuator 800 /spl mu/m in diameter could generate center deflections of around /spl sim/7.0 /spl mu/m, significantly greater than the diaphragm thickness of 2.8 /spl mu/m. The deflection profiles for the diaphragm actuators became flatter when an inactive region in the annular IDT configuration was introduced. There was also a proportional reduction of the maximum deflection.
Reduced Folate Carrier Expression in Acute Lymphoblastic Leukemia: A Mechanism for Ploidy but not Lineage Differences in Methotrexate Accumulation (1999)

Methotrexate (MTX) is one of the most active and widely used agents for the treatment of acute lymphoblastic leukemia (ALL). To elucidate the mechanism for higher accumulation of MTX polyglutamates (MTX-PG) in hyperdiploid ALL and lower accumulation in T-lineage ALL, expression of the reduced folate carrier (RFC) was assessed by reverse transcription-polymerase chain reaction in ALL blasts isolated from newly diagnosed patients. RFC expression exhibited a 60-fold range among 29 children, with significantly higher expression in hyperdiploid B-lineage ALL (median, 11.3) compared with nonhyperdiploid ALL (median, 2.1; P < .0006), but no significant difference between nonhyperdiploid B-lineage and T-lineage ALL. Furthermore, mRNA levels of RFC (mapped by FISH to chromosome 21) were significantly related to chromosome 21 copy number (P = .0013), with the highest expression in hyperdiploid ALL blasts with 4 copies of chromosome 21. To assess the functional significance of gene copy number, MTX-PG accumulation was compared in ALL blasts isolated from 121 patients treated with either low-dose MTX (LDMTX; n = 60) or high-dose MTX (HDMTX; n = 61). After LDMTX, MTX-PG accumulation was highest in hyperdiploid B-lineage ALL with 4 copies of chromosome 21 (P = .011), but MTX-PG accumulation was not significantly related to chromosome 21 copy number after HDMTX (P = .24). These data show higher RFC expression as a mechanism for greater MTX accumulation in hyperdiploid B-lineage ALL and indicate that lineage differences in MTX-PG accumulation are not due to lower RFC expression in T-lineage ALL.
Long-period fiber grating fabrication by high-intensity femtosecond pulses at 211 nm (2005)

Using high-intensity (110-200 GW/cm/sup 2/) 250-fs 211-nm laser pulses and a point-by-point technique, the efficiency of long-period grating inscription in H/sub 2/-loaded standard telecom Corning SMF-28 and H/sub 2/-free photosensitive B-codoped Fibercore fibers was studied and compared with those at other existing recording methods (low-intensity 157-nm, 193-nm, 248-nm or high-intensity 264-nm fabrications). It was shown that at high-intensity 211-nm laser inscription, two-quantum photoreactions are responsible for long-period fiber grating (LPFG) formation, which results in a significant photosensitivity enhancement in comparison with conventional low-intensity 248-nm exposure (by 45 times for SMF-28 fiber). It was found that the grating strength in the case of SMF-28 fiber, irradiated with high-intensity 211-nm pulses, reaches 28 dB, which is the highest value among all known photochemical approaches. The thermal studies of the recorded gratings were also conducted.
[Comparison of the effects of SIN-1, sodium nitroprusside and nitrate derivatives on the inhibition of blood platelet aggregation and activation of soluble platelet guanylate-cyclase]. (1987)

We compared the effects of various nitrates, sodium nitroprusside (SNP), molsidomine, and its bioactive metabolite SIN-1, on platelet aggregation and on the activity of soluble guanylate-cyclase from human platelets. SIN-1 and SNP proved to be potent inhibitors of platelet aggregation and activated soluble guanylate-cyclase in contrast to nitrates and molsidomine which produced weak inhibition of aggregation and failed to activate soluble guanylate-cyclase. These results suggest a correlation between these products' inhibitory effect on aggregation and activation of guanylate-cyclase.
Enhancement of sonodynamic tissue damage production by second-harmonic superimposition: theoretical analysis of its mechanism (1996)

Among the nonthermal effects of ultrasound, acoustic cavitation may have the highest potential for therapeutic applications if it can be somehow controlled. Recent in vitro and in vivo experiments have demonstrated that sonochemically active cavitation can be enhanced an order of magnitude by superimposing the second harmonic onto the fundamental in insonation. Moreover, they have shown that sonochemically active cavitation can be controlled with relative ease, thereby even in a progressive wave field. The effect of second-harmonic superimposition on the rectified diffusion through the gas-liquid interface of cavitated microbubbles is estimated theoretically. The theoretical rectified diffusion rate explained an asymmetric behavior of the threshold for producing sonodynamic tissue damage as a function of the fundamental and the second-harmonic amplitudes. The tissue damage was produced with a focused progressive wave in a liver lobe of a mouse administered with a sonodynamically active agent. The result suggests that the acceleration of the rectified diffusion is a primary mechanism of the enhancement of sonodynamically effective cavitation by second-harmonic superimposition.
PlusTag-Magic medical record system (2003)

PlusTag is a rugged, wearable smart card in the form of a 'dogtag' that can be programmed to carry a patient's medical record, including the most recent X-ray and ECG. The device. together with MAGIC (Medical Applications Graphic Image Compression) software, is intended to aid persons who, because of their disabilities. are unable to provide medical information about themselves in an emergency.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Endothelial‐to‐mesenchymal transition in cardiovascular diseases: Developmental signaling pathways gone awry (2017)

The process named endothelial-to-mesenchymal transition (EndMT) was observed for the first time during the development of the chicken embryo several decades ago. Of interest, accumulating evidence suggests that EndMT plays a critical role in the onset and progression of multiple postnatal cardiovascular diseases. EndMT is controlled by a set of developmental signaling pathways, very similar to the process of epithelial-to-mesenchymal transition, which determine the activity of several EndMT transcriptional effectors. Once activated, these EndMT effectors regulate the expression of endothelial- and mesenchymal-specific genes, in part by interacting with specific motifs in promoter regions, eventually leading to the down-regulation of endothelial-specific features and acquisition of a fibroblast-like phenotype. Important technical advances in lineage tracing methods combined with experimental mouse models demonstrated the pathophysiological importance of EndMT for human diseases. In this review, we discuss the major signal transduction pathways involved in the activation and regulation of the EndMT program. Furthermore, we will review the latest discoveries on EndMT, focusing on cardiovascular diseases, and in particular on its role in vascular calcification, pulmonary arterial hypertension, and organ fibrosis. Developmental Dynamics 247:492-508, 2018. © 2017 Wiley Periodicals, Inc.
Policy Watch: Employment Initiatives (1999)

An exchange of letters between the Prime Minister and his Employment Minister has put employment initiatives on the Government's agenda. This policy watch discusses those letters and their implication for policy over the course of this Parliament and the next.
Morphology of polyamide and polyether block amide blends (1989)

Abstract The morphology of polyamide 12 and polyether block amide blends was investigated by electron microscopy and dynamic mechanical techniques. The blends are not miscible, and phase segregation is observed. In each phase, the lamellar structure of the pure components is observed. The stability of these micro phases is pointed out. When the mass ratio of the hard and soft sequences in the block copolymer is about 4, a partial miscibility takes place.
Amphiphilic property of chlorhexidine and its toxicity against Streptococcus mutans GS-5. (1994)

The toxicity of chlorhexidine digluconate against Streptococcus mutans GS-5 was comparatively determined by measuring its bactericidal activity, and its inhibitory effect on microbial dehydrogenases was studied by the resazurin reduction method. Both methods indicated that chlorhexidine within the range 0.75-5.00 mg/l was highly toxic to Streptococcus mutans, probably due to inhibition of dehydrogenase activity, and the extent of toxicity was closely associated with concentration. In an attempt to better understand the relationship between the amphiphilic nature of chlorhexidine and its toxicity against Streptococcus mutans, the effect of solvent polarity on dehydrogenase inhibition was investigated. A decrease in solvent polarity, induced by inclusion of 5% acetone in the reaction mixture, did not enhance the toxicity of chlorhexidine. This implies that the antimicrobial action of chlorhexidine is mainly attributable to its hydrophilicity, and that the nature of the lipophilic groups is only of secondary importance.