gebhart/patrepeval huggingface dataset:
Ki-67, Cyclin E, and p16 INK4 Are Complimentary Surrogate Biomarkers for Human Papilloma Virus-Related Cervical Neoplasia (2001)

Prior studies of Ki-67, cyclin E, and p16 expression have suggested that these biomarkers may be preferentially expressed in cervical neoplasia. This study examined and compared the distribution of staining for these three antigens in 1) normal and reactive epithelial changes, 2) diagnostically challenging cases (atypical metaplasia and atypical atrophy), 3) squamous intraepithelial lesions (SIL), and 4) high-and low-risk human papilloma virus (HPV) type-specific SIL. One hundred four epithelial foci from 99 biopsies were studied, including low-grade squamous intraepithelial lesions (LSIL; 24), high-grade squamous intraepithelial lesions (HSIL; 36), mature or immature (metaplastic) squamous epithelium (29), and atrophic or metaplastic epithelium with atypia (15). Cases were scored positive for Ki-67 expression if expression extended above the basal one third of the epithelium, for cyclin E if moderate to strong staining was present, and for p16 if moderate to strong diffuse or focal staining was present. HPV status was scored by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of extracted DNA. Immunohistochemical findings were correlated with histologic and viral data. Overall, a histologic diagnosis of SIL correlated strongly with all of the biomarkers used (p <0.001). Positive scores for Ki-67, cyclin E, and p16 were seen in 68.4%, 96.7%, and 100% of LSILs and 94.7%, 91.6%, and 100% of HSILs, respectively. Positive predictive values of these three biomarkers for HPV were 82.4%, 89.5%, and 91.4%, respectively. The positive predictive value for HPV of either cyclin E or p16 was 88.7%. Strong diffuse staining for p16 was significantly associated with high-risk HPV-associated lesions. Normal or reactive epithelial changes scored positive for the three biomarkers in 7.7%, 8.0%, and 12%, respectively. Limitations in specificity included minimal or no suprabasal staining for Ki-67 in immature condylomas and occasional suprabasal staining of reactive epithelial changes (10%), diffuse weak nuclear cyclin E staining in some normal or metaplastic epithelia, and diffuse weak basal p16 staining and occasional stronger focal positivity in normal epithelia. Ki-67, cyclin E, and p16 are complementary surrogate biomarkers for HPV-related preinvasive squamous cervical disease. (Because cyclin E and p16 are most sensitive for LSIL and HSIL [including high-risk HPV], respectively, use of these biomarkers in combination for resolving diagnostic problems, with an appreciation of potential background staining, is recommended.)
On System Safety and Reliability in Early Design Phases : Cost Fo cused Optimization Applied on Aircraft Systems (2013)

System Safety and Reliability are fundamental to system design and involve a quantitative assessment prior to system development. An accurate prediction of reliability and system safety in a new pr ...
Efficient separation, culture and identification of Sertoli cells in new-born calf. (2010)

【Objective】 We used modified method to separate and culture Sertoli cells in order to set up a simple and effective method for separating Sertoli cells.【Method】 The tissue was treated with the methods of separating seminiferous tubule and cutting tissue respectively.Then,the disuse was digested with the 1.0 g/L collagenase and 2.5 g/L trypsin sequentially.The effect of the two methods on the Sertoli cell separation was compared.Sertoli cells were identified according to Feulgen staining and immunocytochemistry.【Result】 After the seminiferous tubule was separated,the digestion period(8 min) was shorter than that(15 min) after the tissue was treated with cutting,the number of effective cells(1.0×107) was more than that(0.81×107) after the tissue was treated with cutting.The satellite karyosomes were stained in violet.The expression of ABP was examined by SABC staining.【Conclusion】 Separating the seminiferous tubule is an efficient way for the Sertoli cell digested with collagenase and trypsin sequentially.
Hollow Cathode Discharges - Analytical Applications (1984)

The low pressure glow discharges considered in this paper are the hollow cathode (Paschen), and the flat cathode (Grimm). Both discharges have similar voltage-current characteristics which are responsible for their radiation stability. The analytical sample is supplied to the discharge through a sputtering mechanism which provides a stable and non-selective source of particles. Some of the fundamental properties of the glow discharge and sputtering phenomena will be discussed, including the relation between the geometry of the discharge, and the nature and pressure of sustaining gas, and current, on the emission characteristics of the discharges. These will he followed by a description of the conventional instrumentation developed for analytical purposes using the hollow cathode and flat discharge. A description of the hollow cathode developed at the National Bureau of Standards (NBS) will follow. The techniques used for the introduction of various conductive and nonconductive materials into the discharge will be discussed. The use of these discharges will be illustrated with examples taken from the literature and from the measurements performed at NBS. The paper will conclude with a discussion of possible future developments of low pressure glow discharges. A collection of references to works on low pressure glow discharges, containing 690 entries, concludes this work.
Ada: constructing a synthetic organism (2002)

Despite immense progress in neuroscience, we remain restricted in our ability to construct autonomous behaving robots that match the competence of even simple animals. The barriers to the realisation of this goal include: the lack of knowledge of system integration issues, engineering limitations and organisational constraints common to many research laboratories. In this paper we describe our approach to addressing these issues by constructing an artificial organism within the framework of the Ada project - a large-scale public exhibit for the Swiss Expo.02 national exhibition.
Injectable Solid Peptide Hydrogel as a Cell Carrier: Effects of Shear Flow on Hydrogels and Cell Payload (2012)

β-hairpin peptide-based hydrogels are a class of injectable solid hydrogels that can deliver encapsulated cells or molecular therapies to a target site via syringe or catheter injection as a carrier material. These physical hydrogels can shear-thin and consequently flow as a low-viscosity material under a sufficient shear stress but immediately recover back into a solid upon removal of the stress, allowing them to be injected as preformed gel solids. Hydrogel behavior during flow was studied in a cylindrical capillary geometry that mimicked the actual situation of injection through a syringe needle in order to quantify effects of shear-thin injection delivery on hydrogel flow behavior and encapsulated cell payloads. It was observed that all β-hairpin peptide hydrogels investigated displayed a promising flow profile for injectable cell delivery: a central wide plug flow region where gel material and cell payloads experienced little or no shear rate, and a narrow shear zone close to the capillary wall where gel and cells were subject to shear deformation. The width of the plug flow region was found to be weakly dependent on hydrogel rigidity and flow rate. Live-dead assays were performed on encapsulated MG63 cells 3 h after injection flow and revealed that shear-thin delivery through the capillary had little impact on cell viability and the spatial distribution of encapsulated cell payloads. These observations help us to fundamentally understand how the gels flow during injection through a thin catheter and how they immediately restore mechanically and morphologically relative to preflow, static gels.
OmniViewer (2015)

This paper presents OmniViewer, a multi-modal 3D video streaming system based on Dynamic Adaptive Streaming over HTTP (DASH) standard. OmniViewer allows users to view arbitrary side of a performer by choosing the view angle from 0° to 360°. Besides, according to the current available bandwidth, it can also adaptively change the bitrate of rendered 3D video for both smooth and high-quality view rendering. Finally, OmniViewer extends traditional DASH implementation to support multi-modal data streaming besides video and audio.
Properties of a 2D fat navigator for prospective image domain correction of nodding motion in brain MRI (2014)

FatNav is advantageous in that it leaves most of the brain water magnetization unaffected and left to the host pulse sequence. Furthermore, high acceleration factors are possible with FatNav, which reduces estimation bias and the navigator duration.
Identification of Multi-Input Multi-Output Linear Systems From Frequency Response Data (1982)

A procedure for identifying a multi-input multi-output linear system from frequency response data is developed. Individual transfer functions from individual input to individual output are identified via the generalized least-squares theory. Orders of the transfer functions are determined by testing the residual. The minimal order multi-input multi-output system is identified based on the formation of a composite system comprising the subsystems associated with all individual outputs followed by decomposition. This is actually the problem of minimal realization involving inexact system parameters.
Polarization Behavior of a Ferroelectric Liquid Crystal Light Valve (1993)

Surface stabilized ferroelectric liquid crystal spatial light modulators (FLCSLM's) might be used in both neural network and optical interconnect applications
Profile Following for Inspection of Underwater Structures (2013)

Abstract We present a seabed profile estimation and following method for close proximity inspection of 3D underwater structures using autonomous underwater vehicles (AUVs). The presented method is used to determine a path allowing the AUV to pass its sensors over all points of the target structure, which is known as coverage path planning. Our profile following method goes beyond traditional seabed following at a safe altitude and exploits hovering capabilities of recent AUV developments. A range sonar is used to incrementally construct a local probabilistic map representation of the environment and estimates of the local profile are obtained via linear regression. Two behavior-based controllers use these estimates to perform horizontal and vertical profile following. We build upon these tools to address coverage path planning for 3D underwater structures using a (potentially inaccurate) prior map and following cross-section profiles of the target structure. The feasibility of the proposed method is demonstrated using the GIRONA 500 AUV both in simulation using synthetic and real-world bathymetric data and in pool trials.
Analysis of the variations, at an intermediate regional scale, of life history traits of Rhinella arenarum using reciprocal transplant experiments (2010)

En los fenomenos de plasticidad, es necesario distinguir experimentalmente el componente
genetico de la variacion inducida por el ambiente y de la interaccion de ambos, que nos permita hacer
interpretaciones acerca de la evolucion y la naturaleza adaptativa de estos cambios. Entre las
aproximaciones experimentales que pueden ayudarnos, suele utilizarse el experimento de trasplante
reciproco, en el cual individuos de diferentes poblaciones son criados de manera conjunta en cada uno de los
ambientes originales. En este trabajo, se presentan los resultados del estudio de las fuentes causales de la
variacion geografica en caracteres de historias de vida durante la etapa larvaria en Rhinella arenarum. Con
este proposito se diseno un experimento de trasplante reciproco en los ambientes originales de Moldes
(ambiente sur) y Salta (ambiente norte), ambos pertenecientes a la unidad geoestructural del Valle de Lerma,
Salta, Argentina. Se observaron respuestas diferenciales de las poblaciones con relacion al ambiente de
crianza como asi tambien influencia de este ultimo en la edad, tamano y tasa de crecimiento de los
individuos metamorficos provenientes de las poblaciones estudiadas.
Truncated TrkB receptor-induced outgrowth of dendritic filopodia involves the p75 neurotrophin receptor (2004)

The Trk family of receptor tyrosine kinases and the p75 receptor (p75NTR) mediate the effects of neurotrophins on neuronal survival, differentiation and synaptic plasticity. The neurotrophin BDNF and its cognate receptor tyrosine kinase, TrkB.FL, are highly expressed in neurons of the central nervous system. At later stages in postnatal development the truncated TrkB splice variants (TrkB.T1, TrkB.T2) become abundant. However, the signalling and function of these truncated receptors remained largely elusive. We show that overexpression of TrkB.T1 in hippocampal neurons induces the formation of dendritic filopodia, which are known precursors of synaptic spines. The induction of filopodia by TrkB.T1 occurs independently of neurotrophin binding and of kinase activity of endogenous TrkB.FL. Coexpression of a p75NTR lacking an intracellular domain inhibits the TrkB.T1-induced effect in a dominant negative manner. Steric hindrance of extracellular p75NTR interactions with a specific antibody, or absence of p75NTR with an intact extracellular domain also inhibit this TrkB.T1-induced effect. We thus propose a novel signalling pathway initiated by neurotrophin-independent extracellular or intramembrane interaction of TrkB.T1 with the p75NTR receptor, which modulates dendritic growth via p75NTR signalling cascades.
PneumaKnit: Actuated Architectures Through Wale- and Course-Wise Tubular Knit-Constrained Pneumatic Systems (2017)

This research explores the development of seamless pneumatically actuated systems whose motion is controlled by the combination of differentially knitted textiles and standardized thin-walled silicone tubing. This work proposes a fundamental material strategy that addresses challenges ranging from soft robotics to pneumatic architecture. Research in soft robotics seeks to achieve complex motions through non-mechanical monolithic systems, comprised of highly articulated shapes molded with a combination of elastic and inelastic materials. Inflatables in architecture focus largely on the active structuring of static forms, as facade systems or as structured envelopes. An emerging use of pneumatic architecture proposes morphable, adaptive systems accomplished through differentiated mechanically interconnected components. In the research described in this paper, a wide array of capabilities in motion and geometric articulation are accomplished through the design of knitted sleeves that generate a series of actuated Âelbows.Â As opposed to molding silicone bladders, differentiation in motion is generated through the more facile ability of changing stitch structure, and shaping of the knitted textile sleeve, which constrains the standard silicone tubing. The relationship between knit differentiation, pneumatic pressure, and the resultant motion profile is studied initially with individual actuators, and ultimately in propositions for larger seamless assemblies. As opposed to a cellular study of individual components, this research proposes structures with multi-scalar articulation, from fiber and stitch to overall form, composed into seamless, massively deformable architectures.
Fast Measurement of Sarcomere Length and Cell Orientation in Langendorff-Perfused Hearts Using Remote Focusing Microscopy (2013)

Remote focusing microscopy can measure cell orientation in complex 2-photon data sets without capturing full z stacks. The technique allows rapid assessment of SL in healthy and diseased heart experimental preparations.
Application of HTML5 multimedia (2012)

HyperText Markup Language (HTML) is the main markup language for web pages. HTML5 is a new generation of HTML standards. With the development of HTML5, it has wide range of applications in the multimedia direction. This new standard first describes the Video and Audio tag, and then elements of Video and Audio are discussed, pointing out that the support of the current browser to Video and Audio format. Then the advantages and disadvantages of HTML5 and flash are discussed, and it pointed out some applications that HTML5 multimedia cannot achieved. And it also introduces the Working principle of the HTTP Live Streaming. Finally, we discussed the direction of development of HTML5 multimedia in the future. HTTP Live Streaming, Audio Data API and peer-to-Peer Networking will become the focus of application of HTML5 multimedia.
Localization of neutralizing epitopes and the receptor-binding site within the amino-terminal 330 amino acids of the murine coronavirus spike protein (1994)

To localize the epitopes recognized by monoclonal antibodies (MAbs) specific for the S1 subunit of the murine coronavirus JHMV spike protein, we have expressed S1 proteins with different deletions from the C terminus of S1. S1utt is composed of the entire 769-amino-acid (aa) S1 protein; S1NM, S1N, S1n(330), and S1n(220) are deletion mutants with 594, 453, 330, and 220 aa from the N terminus of the S1 protein. The expressed S1 deletion mutant proteins were examined for reactivities to a panel of MAbs. All MAbs classified in groups A and B, those reactive to most mouse hepatitis virus (MHV) strains and those specific for isolate JHMV, respectively, recognized S1N(330) and the larger S1 deletion mutants but failed to react with S1N(220). MAbs in group C, specific for the larger S protein of JHMV, reacted only with the S1utt protein without any deletion. These results indicated that the domain composed of the N-terminal 330 aa comprised the cluster of conformational epitopes recognized by MAbs in groups A and B. It was also shown that the epitopes of MAbs in group C were not restricted to the region missing in the smaller S protein. These results together with the fact that all MAbs in group B retained high neutralizing activity suggested the possibility that the N-terminal 330 aa are responsible for binding to the MHV-specific receptors. In investigate this possibility, we expressed the receptor protein and examined the binding of each S1 deletion mutant to the receptor. It was demonstrated that the S1N(330) protein as well as other S1 deletion mutants larger than S1N(330) bound to the receptor. These results indicated that a domain composed of 330 aa at the N terminus of the S1 protein is responsible for binding to the MHV-specific receptor.
Poly(arylenesulfonic acids) with Frozen-In Free Volume as Hydrogen Fuel Cell Membrane Materials (2015)

A new class of proton conducting membranes based on rigid-rod, liquid crystalline, aromatic sulfonic acid polymers, and copolymers was developed at Case Western Reserve University over the past 15 years. Sulfonic acid crowding or incorporation of bulky comonomers forced the chains apart and induced nanoporosity (called frozen in free volume, FFV) that helped in water retention at low external humidity. Very high proton conductivity of these materials made them excellent candidates for fuel cell membranes, especially for operation at elevated temperature and low humidity. Initial work focused on poly(naphthalene bis-tetracarboximides), which were found to be hydrolytically unstable. Then the focus shifted to chemically stable poly(phenylene sulfonic acids), including their copolymers, grafts, and crosslinked derivatives. This paper tracks the study of these remarkable polymers from initial work on polyimides to recent results where polyphenylene membranes were fabricated and tested in a hydrogen/air fuel cell.
Preparation and characteristics of silica-based packings for high-performance liquid chromatography, modified with amino and carboxyl groups (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and characteristics of silica-based packings for high-performance liquid chromatography, modified with amino and carboxyl groupsAkira. Nomura, Joseph. Yamada, and Kinichi. TsunodaCite this: Anal. Chem. 1988, 60, 22, 2509–2512Publication Date (Print):November 15, 1988Publication History Published online1 May 2002Published inissue 15 November 1988https://pubs.acs.org/doi/10.1021/ac00173a016https://doi.org/10.1021/ac00173a016research-articleACS PublicationsRequest reuse permissionsArticle Views218Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
KINETOCARDIOGRAPHY IN CORONARY ARTERY DISEASE (1965)

The beating heart produces low frequency movements of the anterior chest wall. If these movements are recorded with an externally fixed pick-up device a kinetocardiogram (KCG) (Eddleman et al., 1953) is obtained. The curve is a displacement record of the absolute movements and differs in this respect from tracings registered with pick-up devices resting directly upon the chest wall, e.g. apex cardiography (Marey, 1885; Luisada and Magri, 1952; Benchimol, Dimond, and Carson, 1961).
Rhamnolipids, Microbial Virulence Factors, in Alzheimer’s Disease (2017)

Alzheimer's disease (AD) has been attributed to chronic bacterial infections. The recognition of human microbiota as a substantial contributor to health and disease is relatively recent and growing. During evolution, mammals live in a symbiotic state with myriads of microorganisms that survive at a diversity of tissue micro-surroundings. Microbes produce a plethora of secretory products [amyloids, lipopolysaccharides, virulence factors rhamnolipids (RLs), toxins, and a great number of neuroactive compounds]. The contribution of infectious microbial components to the pathophysiology of the human central nervous system including AD is considered potentially substantial, but the involvement of the RLs has never been reported. Here, RLs were isolated from serum and identified through various conventional methods including the colorimetric orcinol method, thin-layer chromatography, attenuated total reflection Fourier transform infrared (ATR-FTIR), and dot blot using antibodies against RLs. Dot blot demonstrated elevated RL levels in sera of AD patients compared to controls (p = 0.014). Moreover, ELISA showed similarly elevated RL levels in cerebrospinal fluid of both AD (0.188 versus 0.080) (p = 0.04) and mild cognitive impairment (0.188 versus 0.129) (p = 0.088) patients compared to healthy, and are well-correlated with the AD stages severity assessed using the Mini-Mental State Examination. These results provide conclusive evidence for the newly-reported implication of RLs in AD, adding it to the list of bacterial components, opening new avenues for AD investigation. Moreover, they strengthen and vindicate the divergence of research toward the exploration of bacterial involvement in AD generation and progression.