gebhart/patrepeval huggingface dataset:
Film redeposition on vertical surfaces during reactive ion etching (1989)

During reactive ion etching of tantalum silicide and polysilicon layers in a chlorine based chemistry it is observed that a film (15–20 nm) is deposited on vertical surfaces of etched structures. This film is even present after common resist strip processes. This film is analyzed by Auger electron spectroscopy and transmission electron spectroscopy, and the formation mechanism is investigated. It is shown it is composed of silicon dioxide sputtered from dielectric layers lying under the etched silicide and poly Si layers. Variables influencing the formation of the residual sidewall film are analyzed. It is demonstrated that the observed residual films are not involved in the anisotropic etch characteristics before the silicide or poly Si layer are etched through. It is further demonstrated that a carbon based polymer film or inhibition layer must be responsible for the anisotropic etch profile of the tantalum silicide layer. This second carbon based film is formed only with a sufficient amount of photoresist in the etching chamber.
Molecular Basis of the Recognition of Nephronectin by Integrin α8β1 (2009)

Integrin alpha8beta1 interacts with a variety of Arg-Gly-Asp (RGD)-containing ligands in the extracellular matrix. Here, we examined the binding activities of alpha8beta1 integrin toward a panel of RGD-containing ligands. Integrin alpha8beta1 bound specifically to nephronectin with an apparent dissociation constant of 0.28 +/- 0.01 nm, but showed only marginal affinities for fibronectin and other RGD-containing ligands. The high-affinity binding to alpha8beta1 integrin was fully reproduced with a recombinant nephronectin fragment derived from the RGD-containing central "linker" segment. A series of deletion mutants of the recombinant fragment identified the LFEIFEIER sequence on the C-terminal side of the RGD motif as an auxiliary site required for high-affinity binding to alpha8beta1 integrin. Alanine scanning mutagenesis within the LFEIFEIER sequence defined the EIE sequence as a critical motif ensuring the high-affinity integrin-ligand interaction. Although a synthetic LFEIFEIER peptide failed to inhibit the binding of alpha8beta1 integrin to nephronectin, a longer peptide containing both the RGD motif and the LFEIFEIER sequence was strongly inhibitory, and was approximately 2,000-fold more potent than a peptide containing only the RGD motif. Furthermore, trans-complementation assays using recombinant fragments containing either the RGD motif or LFEIFEIER sequence revealed a clear synergism in the binding to alpha8beta1 integrin. Taken together, these results indicate that the specific high-affinity binding of nephronectin to alpha8beta1 integrin is achieved by bipartite interaction of the integrin with the RGD motif and LFEIFEIER sequence, with the latter serving as a synergy site that greatly potentiates the RGD-driven integrin-ligand interaction but has only marginal activity to secure the interaction by itself.
Hierarchical nanostructures of cupric oxide on a copper substrate: controllable morphology and wettability (2006)

We have demonstrated a facile fabrication of CuO hierarchical nanostructures on copper substrates by the oxidation of copper in alkaline conditions at 60 °C. CuO flowerlike structures composed of hierarchical two-dimensional (2D) nanosheets and spherical architectures constructed by ultrathin nanowalls of ∼10 nm in thickness could be selectively generated by simply immersing a copper substrate into different alkaline solutions (NaOH and NH3·H2O). The continuous supply of Cu2+ from the substrate, as well as the reaction temperature, has shown its importance for the self-assembled growth of tiny 2D nanosheets and nanowalls into intricate hierarchical nanostructures. Optical absorption was used to determine the band gap energies of the nanostructures. Stable superhydrophobicity was firstly observed for these nanostructured films after modification. When the concentration of solution was changed, well-defined 2D nanosheet and nanowall arrays can accordingly be fabricated on a large scale. Our method might provide a general route towards the preparation of novel hierarchical films on metal substrates (Cu, Al, Zn, etc.), for which a number of promising applications in various fields can be envisioned.
<b>Synthesis of a β-Amyrin Derivative, Olean-11,12;13,18-diene</b> (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of a β-Amyrin Derivative, Olean-11,12;13,18-dieneE. J. Corey, Hans-Jurgen. Hess, and Stephen. ProskowCite this: J. Am. Chem. Soc. 1963, 85, 24, 3979–3983Publication Date (Print):December 1, 1963Publication History Published online1 May 2002Published inissue 1 December 1963https://pubs.acs.org/doi/10.1021/ja00907a016https://doi.org/10.1021/ja00907a016research-articleACS PublicationsRequest reuse permissionsArticle Views311Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A switched current, switched capacitor temperature sensor in 0.6u CMOS (1997)

A Temperature to Digital Converter is described which uses a Sensor based on the principle of switching accurately scaled currents in the parasitic substrate PNP in a standard fine-line CMOS process. The resulting PTAT δV BE signal is amplified in an Auto-Zeroed Switched-Capacitor circuit, sampled and converted to a Digital output by a low power 10 Bit ADC providing a resolution of 0.25 degree from-55 to 125 degrees with an error of less than 1 degree. The paper will focus on the design of the Sensor.
Antagonists of growth hormone-releasing hormone cross the blood-brain barrier: A potential applicability to treatment of brain tumors (2005)

Hypothalamic growth hormone (GH)-releasing hormone (GHRH) stimulates the synthesis and release of GH from the pituitary gland. GHRH and its mRNA are also found in human cancers of the breast, ovary, prostate, lung, and other tumors, suggesting that GHRH is also a tumor growth factor. Various studies show that GHRH antagonists have antiproliferative effects in many tumor models; however, glioblastomas were examined only recently. Previous studies have demonstrated that s.c. administration of GHRH antagonist (JV-1-36) inhibited growth of s.c. U-87MG human glioblastomas and increased survival of nude mice with orthotopic implants of glioblastomas. Although treatment with JV-1-36 reduced tumorigenicity, it is not known whether peripherally administered GHRH antagonists can cross the blood-brain barrier. Brain endothelial cells joined by tight junctions form the blood-brain barrier, a "barrier" between the general circulation and the CNS. In this study, we administered a GHRH antagonist (JV-1-42) and showed that, after i.v. injection, iodinated JV-1-42 (131I-JV-1-42) enters the brain intact at a rate of 0.8514 mocrol/g per min with a serum half-life of 12.2 min. A one-site binding hyperbolic model indicated that the maximal percent of i.v. dose taken up per gram of brain was 0.41%. Coinjection of unlabeled JV-1-42 indicated that the transport from blood to brain is not saturable; however, transport from brain to blood is saturable and involves P-glycoprotein. Taken together, these results demonstrate that i.v.-administered 131I-JV-1-42 readily crosses the blood-brain barrier and accumulates in the brain. This finding indicates that GHRH antagonists could provide a potential treatment for malignant glioblastomas.
A Study of MatchPyramid Models on Ad-hoc Retrieval (2016)

Deep neural networks have been successfully applied to many text matching tasks, such as paraphrase identification, question answering, and machine translation. Although ad-hoc retrieval can also be formalized as a text matching task, few deep models have been tested on it. In this paper, we study a state-of-the-art deep matching model, namely MatchPyramid, on the ad-hoc retrieval task. The MatchPyramid model employs a convolutional neural network over the interactions between query and document to produce the matching score. We conducted extensive experiments to study the impact of different pooling sizes, interaction functions and kernel sizes on the retrieval performance. Finally, we show that the MatchPyramid models can significantly outperform several recently introduced deep matching models on the retrieval task, but still cannot compete with the traditional retrieval models, such as BM25 and language models.
On extracting feature models from product descriptions (2012)

In product line engineering, domain analysis is the process of analyzing related products to identify their common and variable features. This process is generally carried out by experts on the basis of existing product descriptions, which are expressed in a more or less structured way. Modeling and reasoning about product descriptions are error-prone and time consuming tasks. Feature models (FMs) constitute popular means to specify product commonalities and variabilities in a compact way, and to provide automated support to the domain analysis process. This paper aims at easing the transition from product descriptions expressed in a tabular format to FMs accurately representing them. This process is parameterized through a dedicated language and high-level directives (e.g., products/features scoping). We guarantee that the resulting FM represents the set of legal feature combinations supported by the considered products and has a readable tree hierarchy together with variability information. We report on our experiments based on public data and characterize the properties of the derived FMs.
High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis (2016)

This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for the analysis of sub-microliter sample volumes. The wax-printing approach widely used for the patterning of paper substrates has been adapted to obtain high-resolution microfluidic structures patterned in filter paper. This has been achieved by replacing the hot plate heating method conventionally used to melt printed wax features into paper by simple hot lamination. This patterning technique, in combination with the consideration of device geometry and the influence of cellulose fiber direction in filter paper, led to a model µPAD design with four microfluidic channels that can be filled with as low as 0.5 µL of liquid. Finally, the application to a colorimetric model assay targeting total protein concentrations is shown. Calibration curves for human serum albumin (HSA) were recorded from sub-microliter samples (0.8 µL), with tolerance against ±0.1 µL variations in the applied liquid volume.
Negative Pressure Wound Therapy and its Application to Orthopaedics. Part II: Clinical Application (2006)

Negative pressure wound therapy (NPWT) converts an open wound into a controlled closed environment by applying intermittent or continuous sub-atmospheric pressure to a specialized wound dressing. This is sealed with an adhesive dressing that contains the negative pressure at the wound and thereby stimulates healing. This technique has revolutionized the care of acute and chronic open wounds in orthopaedics. Normal wound healing and the current recommendations for the care of orthopaedic wounds using NPWT are reviewed.
The Agrobacterium tumefaciens virB4 gene product is an essential virulence protein requiring an intact nucleoside triphosphate-binding domain (1993)

Products of the approximately 9.5-kb virB operon are proposed to direct the export of T-DNA/protein complexes across the Agrobacterium tumefaciens envelope en route to plant cells. The presence of conserved nucleoside triphosphate (NTP)-binding domains in VirB4 and VirB11 suggests that one or both proteins couple energy, via NTP hydrolysis, to T-complex transport. To assess the importance of VirB4 for virulence, a nonpolar virB4 null mutation was introduced into the pTiA6NC plasmid of strain A348. The 2.37-kb virB4 coding sequence was deleted precisely by oligonucleotide-directed mutagenesis in vitro. The resulting delta virB4 mutation was exchanged for the wild-type allele by two sequential recombination events with the counterselectable Bacillus subtilis sacB gene. Two derivatives, A348 delta B4.4 and A348 delta B4.5, sustained a nonpolar deletion of the wild-type virB4 allele, as judged by Southern blot hybridization and immunoblot analyses with antibodies specific for VirB4, VirB5, VirB10, and VirB11. Transcription of wild-type virB4 from the lac promoter restored virulence to the nonpolar null mutants on a variety of dicotyledonous species, establishing virB4 as an essential virulence gene. A substitution of glutamine for Lys-439 and a deletion of Gly-438, Lys-439, and Thr-440 within the glycine-rich NTP-binding domain (Gly-Pro-Iso-Gly-Arg-Gly-Lys-Thr) abolished complementation of A348 delta B4.4 or A348 delta B4.5, demonstrating that an intact NTP-binding domain is critical for VirB4 function. Merodiploids expressing both the mutant and wild-type virB4 alleles exhibited lower virulence than A348, suggesting that VirB4, a cytoplasmic membrane protein, may contribute as a homo- or heteromultimer to A. tumefaciens virulence.
Characterization and comparison of noise generation for quasi-resonant and pulsewidth-modulated converters (1994)

A buck converter with a given output filter is operated with pulse-width modulated and quasi-resonant switching schemes at the same nominal load and switching frequency. Electromagnetic interference generated by the natural switching action of the converter is examined by spectral analysis. Interference caused by excitation of parasitic elements is examined experimentally. Quasi-resonant converters are found to have a lower switching frequency harmonic bandwidth than the equivalent pulse-width modulated converter, even with switching frequency control. The most significant parasitic responses are the turn-on current and turn-off voltage of the catch diode and the gate current of the MOSFET. A significant decrease in radiated and conducted noise occurs when the gate drive voltage rise and fall times are increased, which is possible without loss of efficiency using quasi-resonant switching.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Sucrochemistry. Part 33. The selective pivaloylation of sucrose (1984)

The pivaloylation of sucrose by pivaloyl chloride (2,2-dimethylpropanoyl chloride) has been studied under a variety of conditions and shown to be selective for certain hydroxy groups. There exists two principal, but divergent, reaction pathways lying between sucrose and its octapivalate in which the orders of acylation of the eight hydroxy groups are as follows (a) 6,6′-OH > 1′-OH > 4′-OH > 2-OH > 4-OH > 3′-OH > 3-OH; (b) 6,6′-OH > 1′-OH > 3′-OH > 3-OH > 4′-OH > 2-OH and 4-OH. With the exception of the 1′,3′,6,6′-tetra- and the 1′,2,3′,4,4′,6,6′-hepta-pivalate, all of the intermediary esters in these two reaction pathways have been isolated in yields in the range 30–52% depending upon the conditions employed.
Differentiation and maturation of growth factor expanded human hematopoietic progenitors assessed by multidimensional flow cytometry. (1992)

Non-adherent cord blood and bone marrow mononuclear cells were analyzed by multiparameter flow cytometry before and at day 2, 4, 7, and 11 of culture in recombinant interleukin 3 (IL-3) and granulocyte colony-stimulating factor (G-CSF, cord blood) or stem cell factor (SCF), IL3 and granulocyte-macrophage colony-stimulating factor (GM-CSF, BM) to assess the differentiation and maturational pathway of myeloid cells. Before cell culture cord blood contained progenitor cells (CD34+) in various differentiation stages (CD38(-)----CD38bright), mature lymphocytes, monocytes, and neutrophils, but no immature neutrophils and immature monocytes. During cell culture, all CD34+ cells acquired the CD38 antigen between day 2 and 5 of cell culture, the CD34 antigen was lost between day 5 and 11 of cell culture. Differentiation of cells into the myeloid cell lineage was characterized by the acquisition of both CD33 and CD71. The latter is indicative for the active proliferation of these cells. Maturation of the cells into the neutrophilic pathway was indicated by the acquisition of first the CD15 antigen followed by CD11b and CD16 respectively. Whereas maturation of the cells into the monocytic pathway was indicated by the acquisition of first CD11b followed by CD14 and a dim expression of both CD15 and CD16. In normal bone marrow, cells of various maturational stages are already present before cell culture. During cell culture differentiation of cells into the myeloid lineage and maturation of the cells along the monocyte and neutrophilic lineage followed identical pathways as was observed before cell culture. Differentiation and maturational pathways of cord blood and adult bone marrow were identical. The results confirm the surface-antigen-defined pathways of myeloid cell differentiation described previously for non-cultured normal bone marrow aspirates. The detailed assessment of cell maturation and differentiation of cultured cells by multidimensional flow cytometry permits the determination of the specific effects of various recombinant human growth factors on myeloid cells.
Learning Action Representations for Reinforcement Learning (2019)

Most model-free reinforcement learning methods leverage state representations (embeddings) for generalization, but either ignore structure in the space of actions or assume the structure is provided a priori. We show how a policy can be decomposed into a component that acts in a low-dimensional space of action representations and a component that transforms these representations into actual actions. These representations improve generalization over large, finite action sets by allowing the agent to infer the outcomes of actions similar to actions already taken. We provide an algorithm to both learn and use action representations and provide conditions for its convergence. The efficacy of the proposed method is demonstrated on large-scale real-world problems.
Enhanced patency of small-diameter, externally supported Dacron iliofemoral grafts seeded with endothelial cells. (1982)

Fourteen adult foxhounds underwent bilateral iliofemoral bypasses with externally supported, knitted Dacron grafts measured 4 mm in internal diameter and 10 cm in length. These conduits were preclotted with 10 ml of blood mixed with 0.5 ml of culture medium. Autologous endothelial cells, enzymatically derived from external jugular veins, were added to blood and medium used to preclot one graft in each dog. The other, unseeded graft served as a control. Grafts were anastomosed, end to end, to the iliac and femoral arteries. All dogs received dipyridamole, 50 mg twice a day for 4 days preoperatively, and aspirin, 5 grains four times a day for 1 day preoperatively. Both drugs were continued 14 days after operation. Grafts were removed from three dogs at 2 and 4 weeks and from four dogs at 8 and 16 weeks. All grafts were patent at 2 weeks during drug administration. Cumulative patency rates beyond 2 weeks were 73% in 11 seeded grafts and 27% in 11 control grafts, a statistically significant difference (P = 0.03). Seeded grafts were completely surfaced with a monolayer of endothelium between 2 and 4 weeks. Small-graft patency appeared related to evolution of endothelial surfaces, the development of which was clearly facilitated by seeding with autologous endothelium.
<i>Xanthomonas campestris</i>Contains a Cluster of<i>hrp</i>Genes Related to the Larger<i>hrp</i>Cluster of<i>Pseudomonas solanacearum</i> (1991)

All Xanthomonas campestris pathovars tested contain DNA which hybridizes to the large hrp gene cluster of Pseudomonas solanacearum (C.A. Boucher, F. Van Gijsegem, P.A. Barberis, M. Arlat, and C. Zischek, J. Bacteriol. 169:5626-5632, 1987). Clones carrying these sequences were isolated from genomic libraries of X. campestris pvs. campestris and vitians. Mutagenesis of the corresponding genomic regions of both pathovars gave strains defective in both pathogenicity and hypersensitive response induction. X. c. pv. campestris contained a hrp gene cluster covering about 25 kb, which was homologous and colinear over a continuous 19-kb DNA region with the P. solanacearum hrp cluster. Cross-complementation showed that X. c. pv. vitians and X. c. pv. campestris hrp sequences are functionally interchangeable, but the source of the hrp genes did not determine the compatibility-incompatibility of the host-pathogen interaction. One X. c. pv. campestris Hrp- mutant was "complemented" by specific subclones of the P. solanacearum hrp cluster, suggesting the existence of some functional homology between the clusters of the two species. Expression of hrp genes (studied by lacZ fusions) was repressed in rich medium, and in minimal medium the level of expression depended on the carbon source supplied to the cells. Transcription of hrp genes was not regulated by genes that control the synthesis of extracellular enzymes, which are required for pathogenicity. In addition X. campestris Hrp- mutants produced wild-type levels of these extracellular enzyme activities. These results suggest the existence of two independent sets of pathogenicity genes that are regulated differently.
Dendritic Cell-Targeted Vaccines (2014)

Despite significant effort, the development of effective vaccines inducing strong and durable T-cell responses against intracellular pathogens and cancer cells has remained a challenge. The initiation of effector CD8(+) T-cell responses requires the presentation of peptides derived from internalized antigen on class I major histocompatibility complex molecules by dendritic cells (DCs) in a process called cross-presentation. A current strategy to enhance the effectiveness of vaccination is to deliver antigens directly to DCs. This is done via selective targeting of antigen using monoclonal antibodies directed against endocytic receptors on the surface of the DCs. In this review, we will discuss considerations relevant to the design of such vaccines: the existence of DC subsets with specialized functions, the impact of the antigen intracellular trafficking on cross-presentation, and the influence of maturation signals received by DCs on the outcome of the immune response.