gebhart/patrepeval huggingface dataset:
Thermography for disease detection in livestock: A scoping review (2022)

Infra-red thermography (IRT) offers potential opportunities as a tool for disease detection in livestock. Despite considerable research in this area, there are no common standards or protocols for managing IRT parameters in animal disease detection research. In this review, we investigate parameters that are essential to the progression of this tool and make recommendations for their use based on the literature found and the veterinary thermography guidelines from the American Academy of Thermology. We analyzed a defined set of 109 articles concerned with the use of IRT in livestock related to disease and from these articles, parameters for accurate IRT were identified and sorted into the fields of camera-, animal- or environment-related categories to assess the practices of each article in reporting parameters. This review demonstrates the inconsistencies in practice across peer-reviewed articles and reveals that some important parameters are completely unreported while others are incorrectly captured and/or under-represented in the literature. Further to this, our review highlights the lack of measured emissivity values for live animals in multiple species. We present guidelines for the standards of parameters that should be used and reported in future experiments and discuss potential opportunities and challenges associated with using IRT for disease detection in livestock.
A CMOS Chopper Amplifier (1986)

This paper presents a CMOS chopper amplifier realized with a 2nd order low-pass selective amplifier, using continuous-time filtering technique. The circuit has been integrated in a 3 μm p-well low-voltage CMOS technology. The chopper DC gain is 32 dB with 200 Hz bandwidth. The equivalent low-frequency input noise is 63 nV/√Hz and free from 1/f noise. The input offset is typically 5 μV. The amplifier consumes only 34 μW and is therefore well suited for biomedical applications, like electrogram amplification.
[Study of biosynthesis of mycoheptin, mycopentene and fatty acids by Streptoverticillium mycoheptinicum strain 255 in various conditions of its cultivation]. (1992)

The effect of the fat content in the medium for cultivation of Streptoverticillium mycoheptinium-255 and the effect of glucose intake on the rate of carbohydrate utilization and intensity of incorporation of labeled sodium acetate and propionate to mycoheptin, mycopentene and fatty acids were studied. It was shown that a lower fat content in the medium in comparison with the optimal one resulted in intensification carbohydrate catabolism during an idiophase, increased production rates of mycopentene, an accompanying antibiotic, and fatty acids and a decrease in the heptaene/pentaene ratio. The optimal contents of the oil in the medium provided a decrease in the rate of the carbohydrate consumption and preferable production of the required antibiotic or mycoheptin accompanied by a decrease in the synthesis rate of mycopentene and fatty acids.
Human cultured skin fibroblasts express estrogen receptor α and β (2002)

Human skin fibroblasts may be the target cells for estrogens. The aim of present study was to confirm the presence of both isoforms of estrogen receptors (ER) in these cells. Experiments were carried out in primary cultures of human skin fibroblasts. ER-alpha and ER-beta mRNAs were measured by quantitative assays based on reverse transcription (RT) of the mRNA and polymerase chain reaction (PCR) amplification of the cDNA. To determine which of the ER isoforms were present and their intracellular locations immunohistochemical staining was performed. MCF-7 culture was a positive control for the immunostaining. The distribution immunostaining of ER-beta protein differed from that of ER-alpha in skin fibroblasts. ER-alpha was detected in both the cytosolic and nuclear compartments of fibroblasts. ER-beta was weakly detectable and was found predominantly in the nuclear compartment. Using the RT-PCR technique mRNA of both ERs was successfully detected in the skin fibroblast cultures with predominantly higher mean level of ER-beta mRNA expression than ER-alpha mRNA. In human culture skin fibroblasts ER-beta co-expresses with ER-alpha. The dominant expression of ER-beta in cultured female skin fibroblasts suggests that ER-beta may play a dominant role in collaboration with ER-alpha in the regulation of estrogen action in skin.
Both CD4<sup>+</sup> FoxP3<sup>+</sup> and CD4<sup>+</sup> FoxP3<sup>−</sup> T cells from patients with B‐cell malignancy express cytolytic markers and kill autologous leukaemic B cells <i>in vitro</i> (2011)

Cytotoxic CD4(+) T cells have been found in patients with chronic lymphocytic leukaemia (CLL) and seem to be involved in the regulation of malignant B cells. The CD4(+) T regulatory cells (Tregs) can regulate various immune cells, including B cells, by inducing their apoptosis. Hence, different subgroups of CD4(+) T cells may be involved in the regulation of malignant B cells. In this study, the cytotoxic phenotype and function of various CD4(+) T-cell subgroups were investigated in patients with B-cell malignancies. Peripheral blood was collected from patients with CLL, various B-cell lymphomas, healthy adult donors, children with precursor B-cell acute lymphoblastic leukaemia (pre-B ALL) and from healthy children. CD4(+) T cells (CD3(+) CD4(+) FoxP3(-)), Tregs (CD3(+) CD4(+) CD127(low) FoxP3(+)) and CD127(high) FoxP3(+) T cells (CD3(+) CD4(+) CD127(high) FoxP3(+)) were analysed for their expression of the cytolytic markers CD107a and Fas ligand. Patients with CLL had increased CD107a expression on all tested T-cell subgroups compared with healthy donors. Similar results were found in patients with B-cell lymphomas whereas the CD107a expression in children with pre-B ALL was no different from that in healthy controls. Fas ligand expression was similar between patient cells and cells of healthy donors. CD4(+) T cells and Tregs from patients with CLL and healthy donors were subsequently purified and cultured in vitro with autologous B cells. Both subgroups lysed B cells and killing was confirmed by granzyme ELISAs. In conclusion, cytotoxic populations of CD4(+) T cells, including Tregs, are present in patients with B-cell malignancy and may be an important factor in immune-related disease control.
Under Field Oxide Dopant Enhancement (UFDE) for CMOS and BiCMOS Technology (1990)

A structure with n-type dopant enhancement only under field oxide in an n-well and its implementation are proposed. This technique was applied to 3 µm CMOS and BiCMOS technology, and it was shown that CMOS and BiCMOS latch-up and collector resistance of the bipolar transistor were improved by adopting this technology. Problems with regards to this technology are also addressed.
Maize genetic resources (1995)

This publication documents the origin and worldwide spread of maize from Mesoamerica, and describes the characterization, use, and conservation of maize genetic resources, including the crop's nearest wild relatives, teosinte and Tripsacum. Results of an international survey on the continued use of maize landraces in developing countries are reported, along with progress on the work of Latin American maize banks, in concert with CIMMYT, USAID, and USDA-NSSL, to regenerate their endangered holdings of maize landraces. An update is provided on collaborative research by CIMMYT and the French National Research Institute for Development Cooperation (ORSTOM) to transfer apomixis from Tripsacum to maize. Correct citation: Taba, S. (ed.). 1995. Maize Genetic Resources. Maize Program Special Report. Mexico, D.F. : CIMMYT. ISBN: 968-6923-48-9 AGROVOC descriptors: Zea mays; Euchlaena mexicana; Tripsacum; Land races; Provenance; Natural distribution; Genetic resources; Germplasm conservation; Apomixis ACRIS subject codes: F30 Dewey decimal classification: 633.153
Migraine with Visual Aura and Photosensitive Epileptic Seizures (1997)

A relationship between epilepsy and migraine has long been postulated, but the nature of this interaction is still debated. We observed this association in a 23-year old man with a history of migraine with visual aura who had seizures at age 15 years while watching television. Waking EEG was normal, but sleep recordings demonstrated posterior spike-waves during sleep. During intermittent photic stimulation (IPS), a photoparoxysmal response occurred, maximum in occipital areas. Brain magnetic resonance imaging scan was normal. Seizures did not recur after 4-year treatment with valproate. He is currently seizure-free, but continues to have rare migraine with visual aura. The role of spreading depression or of a putative dopaminergic failure in occipital cortex is discussed.
Five Priorities for the New Metro Strategy (2011)

Too few people really understood, let alone embraced, the strategic directions and planning concepts contained in Melbourne 2030. The former State Government failed in 'selling' the strategy to the wider community at the outset leaving local councils at the coalface of community backlash to urban intensification and densification. Melbourne@5million irritated even more the tensions between state and local governments, fuelling vigorous debate about how many people Melbourne can, should or will accommodate.
Time-Optimal Design of a CMOS Adder (2005)

In this paper, we present a systematic method of implementing a VLSI parallel adder. First, we define a family of adders, based on a modular design. Our design uses three types of component cells, which we implement in static CMOS. We then formulate the adder design as a dynamic programming problem, optimizing with respect to time. As a result, we have found the fastest 32-bit CMOS adder in our design family.
Encapsulation and controlled release of biomolecules from silica microparticles (2006)

Biomolecules have been encapsulated in silica microparticles for controlled release applications, by destabilisation of aqueous silica precursors in the water droplets of a water-in-oil emulsion. The use of colloidal solutions (colloids typically in the size range 6–20 nm) as silica precursors avoids the detrimental effect of alcohol in the reactant solution. Thus, subtilisin A has been encapsulated with little to no loss in enzymatic activity. The particles are mesoporous, with a well-defined pore-size in the order of the size of most proteins, enabling the molecules to diffuse out of the solid. The release rate can be controlled by adjusting the pore-size of the silica particles.
New convenient defined media for [35S]methionine labelling and proteomic analyses of probiotic lactobacilli (2006)

Efficient tools for proteomic analyses of probiotic Lactobacillus were developed and applied for stress-response studies.
Phagocytosis: An Overview (1987)

Review Articles| October 01 2008 Phagocytosis: An Overview Subject Area: Immunology and Allergy , Pathology and Cell Biology Bulbul Chakravarti; Bulbul Chakravarti Department of Immunology, Research Institute of Scripps Clinic, La Jolla, Calif., USA Search for other works by this author on: This Site PubMed Google Scholar Deb N. Chakravarti Deb N. Chakravarti Department of Immunology, Research Institute of Scripps Clinic, La Jolla, Calif., USA Search for other works by this author on: This Site PubMed Google Scholar Pathology and Immunopathology Research (1987) 6 (5-6): 316–342. https://doi.org/10.1159/000157062 Article history Published Online: October 01 2008 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Bulbul Chakravarti, Deb N. Chakravarti; Phagocytosis: An Overview. Pathology and Immunopathology Research 31 December 1987; 6 (5-6): 316–342. https://doi.org/10.1159/000157062 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsSurvey and Synthesis of Pathology Research Search Advanced Search This content is only available via PDF. 1987Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Article PDF first page preview Close Modal You do not currently have access to this content.
Hydrolysis of Synthetic Substrate, <small>L</small>-Pyroglutamyl <i>p</i>-Nitroanilide is Catalyzed Solely by Pyroglutamyl Aminopeptidase I in Rat Liver Cytosol (2003)

Pyroglutamyl aminopeptidase I (PAP-I) is a cytosolic cysteine peptidase, which hydrolytically removes the L-pyroglutamate residue from the amino terminus of endogenous proteins and peptides. L-Pyroglutamyl p-nitroanilide serves as the synthetic substrate of this enzyme, while there is a possibility of other hydrolases being involved in the hydrolysis of this xenobiotic substrate. We cloned a full-length cDNA encoding rat PAP-I from a rat liver cDNA library and expressed this cDNA in Escherichia coli to obtain a recombinant PAP-I as a single protein. The cDNA encoded a sequence of 209 amino acids with a calculated molecular weight of 22913 Da. The homology of the deduced amino acid sequence of rat PAP-I was 98.6 and 94.3% to mouse and human PAP-Is, respectively. The biochemical properties of the recombinant rat PAP-I were almost identical to those of the recombinant mouse and human PAP-Is and the purified rat liver cytosolic PAP-I in terms of the molecular weight, subunit structure, affinity to the substrate, inhibitor profile and pH optimum. Immunoblot analysis using an antibody raised against recombinant rat PAP-I showed that rat PAP-I is present almost exclusively in the cytosolic fraction of the rat liver. Moreover, the hydrolyzing activity for L-pyroglutamyl p-nitroanilide in rat liver cytosolic fraction was completely inhibited by the antibody, strongly suggesting that this xenobiotic substrate is hydrolyzed solely by PAP-I.
[An analysis of main causes of low efficiency of rendering care for pyo-necrotic complications of syndrome of the diabetic foot]. (2007)

In the city of Chelyabinsk during 1996 through 2003 there was growing incidence of necrotic complications of syndrome of the diabetic foot (SDF) from 26.0 up to 50.5 per 100 000 adults (p < or = 0.001). According to prognosis the incidence of SDF will grow by 2010 more than two times (R2 = 0.9849). Comparison of effectiveness of surgical treatment on Diabetological centers (1st group) and non-specialized institutions (2nd group) has shown that lethality in the first group was 4.2%, in the second group--9.7%. In the first group the frequency of high amputations decreased from 31.3% in 1996 to 12.3% in 2005 (p < 0.05). As a whole, organizational, diagnostic and medical defects of treatment were noted in 89.5 out of 100 patients in both groups, the errors in specialized institutions being made 5.7 times more rarely than in non-specialized ones, that speaks well to specialized Diabetological centers.
Introduction of Carbogenic Substituent into the 4-Position of 1-Methyl-1H-imidazole. (1994)

The 4-position of the 1-methyl-1H-imidazole ring was lithiated by treatment of the corresponding 4-bromo derivatives with tert-butyllithium. This procedure was applied to synthesis of the left-hand part of pyronaamidine (1), which is a biologically interesting marine alkaloid.
Effects of inulin on faecal bifidobacteria in human subjects (1999)

A controlled study with eight healthy free-living subjects was carried out, in which energy intake was adjusted to the individual energy requirements. On administration of inulin, blood lipids, the faecal microflora, short-chain fatty acids and accompanying gastrointestinal symptoms were characterized in order to investigate the long-term effect of inulin. During the run-in phase (8 d), subjects received a typical Western diet providing 45% energy as fat and 40% energy as carbohydrate. Subsequently, the subjects consumed a fat-reduced diet which provided 30% energy as fat and 55% energy as carbohydrate for a period of 64 d using inulin as a fat replacer. The amounts of inulin consumed by the subjects (up to 34 g/d) were based on individual energy requirements with the aim to keep the diet isoenergetic with that used in the run-in period. To assess the effects of inulin administration, a control study (run-in and intervention) was carried out in which subjects consumed the same diet but devoid of inulin during the whole course of the study. To investigate the effect of inulin on faecal flora composition total bacteria and bifidobacteria in the faeces were enumerated by in situ hybridization with 16S rRNA targeted oligonucleotide probes. Inulin significantly increased bifidobacteria from 9.8 to 11.0 log10/g dry faeces and caused a moderate increase in gastrointestinal symptoms such as flatulence and bloatedness, whereas blood lipids and short-chain fatty acids remained essentially unaffected.
Mice Deficient in Nuclear Factor (NF)-κB/p52 Present with Defects in Humoral Responses, Germinal Center Reactions, and Splenic Microarchitecture (1998)

p52 is a subunit of nuclear factor (NF)-kappa B transcription factors, most closely related to p50. Previously, we have shown that p52, but not p50 homodimers can form transactivating complexes when associated with Bcl-3, an unusual member of the I kappa B family. To determine nonredundant physiologic roles of p52, we generated mice deficient in p52. Null mutant mice were impaired in their ability to generate antibodies to T-dependent antigens, consistent with an absence of B cell follicles and follicular dendritic cell networks in secondary lymphoid organs, and an inability to form germinal centers. Furthermore, the splenic marginal zone was disrupted. These phenotypes are largely overlapping with those observed in Bcl-3 knockout animals, but distinct from those of p50 knockouts, supporting the notion of a physiologically relevant complex of p52 homodimers and Bcl-3. Adoptive transfer experiments further suggest that such a complex may be critical in accessory cell functions during antigen-specific immune reactions. Possible roles of p52 and Bcl-3 are discussed that may underlie the oncogenic potential of these proteins, as evidenced by recurrent chromosomal translocations of their genes in lymphoid tumors.
A High-Throughput Method for Characterizing Novel Chimeric Antigen Receptors in Jurkat Cells (2020)

Chimeric antigen receptor (CAR) development involves extensive empirical characterization of antigen-binding domain (ABD)/CAR constructs for clinical suitability. Here, we present a cost-efficient and rapid method for evaluating CARs in human Jurkat T cells. Using a modular CAR plasmid, a highly efficient ABD cloning strategy, plasmid electroporation, short-term co-culture, and flow-cytometric detection of CD69, this assay (referred to as CAR-J) evaluates sensitivity and specificity for ABDs. Assessing 16 novel anti-CD22 single-chain variable fragments derived from mouse monoclonal antibodies, CAR-J stratified constructs by response magnitude to CD22-expressing target cells. We also characterized 5 novel anti-EGFRvIII CARs for preclinical development, identifying candidates with varying tonic and target-specific activation characteristics. When evaluated in primary human T cells, tonic/auto-activating (without target cells) EGFRvIII-CARs induced target-independent proliferation, differentiation toward an effector phenotype, elevated activity against EGFRvIII-negative cells, and progressive loss of target-specific response upon <i>in vitro</i> re-challenge. These EGFRvIII CAR-T cells also showed anti-tumor activity in xenografted mice. In summary, CAR-J represents a straightforward method for high-throughput assessment of CAR constructs as genuine cell-associated antigen receptors that is particularly useful for generating large specificity datasets as well as potential downstream CAR optimization.
Nanosphere lithography based technique for fabrication of large area well ordered metal particle arrays (2012)

Nanosphere lithography is an effective technique for high throughput fabrication of well-ordered patterns, but expanding the method to large area coverage of nanoparticles less than 300 nm in diameter while maintaining good order has proven challenging. Here we demonstrate a nanosphere lithography based technique for fabricating large area, wellordered arrays of hemispherical metal particles which pushes the limits of these size constraints. First, large area monolayers of polystyrene (PS) nanospheres are assembled at an air-water interface and then transferred to a submerged substrate. The submerged substrate is supported at a 10° angle so that the water draining speed can be used to control the transfer rate, which is essential for hydrophobic substrates such as the polymer-coated glass used in our work. A double liftoff procedure was used to transfer the PS pattern to a silver particle array on an arbitrary substrate, achieving tunable control over the final metal particle diameter and spacing in the range of 50-150 nm and 100-200 nm, respectively. Additional control over particle shape and diameter can be obtained by modifying the substrate surface energy. For example, depositing silver on ITO-coated glass rather than a more hydrophilic clean glass substrate leads to a more hemispherical particle shape and a diameter reduction of 20%. Peak wavelength-selective reflection greater than 70% and total extinction greater than 90% were measured. The intensity, position and bandwidth of the main plasmon resonance of the arrays were shown to have minimal angle dependence up to at least 30° off normal.