gebhart/patrepeval huggingface dataset:
Gecko: contention-oblivious disk arrays for cloud storage (2013)

Disk contention is increasingly a significant problem for cloud storage, as applications are forced to co-exist on machines and share physical disk resources. Disks are notoriously sensitive to contention; a single application's random I/O is sufficient to reduce the throughput of a disk array by an order of magnitude, disrupting every other application running on the same array. Log-structured storage designs can alleviate write-write contention between applications by sequentializing all writes, but have historically suffered from read-write contention triggered by garbage collection (GC) as well as application reads. Gecko is a novel log-structured design that eliminates read-write contention by chaining together a small number of drives into a single log, effectively separating the tail of the log (where writes are appended) from its body. As a result, writes proceed to the tail drive without contention from either GC reads or first-class reads, which are restricted to the body of the log with the help of a tail-specific caching policy. Gecko trades-off maximum contention-free sequential throughput from multiple drives in exchange for a stable and predictable maximum throughput from a single uncontended drive, and achieves better performance compared to native log-structured or RAID based systems for most cases. Our in-kernel implementation provides random write bandwidth to applications of 60 to 120MB/s, despite concurrent GC activity, application reads, and an adversarial workload.
Spatio–Temporal Image Representation of 3D Skeletal Movements for View-Invariant Action Recognition with Deep Convolutional Neural Networks (2019)

Designing motion representations for 3D human action recognition from skeleton sequences is an important yet challenging task. An effective representation should be robust to noise, invariant to viewpoint changes and result in a good performance with low-computational demand. Two main challenges in this task include how to efficiently represent spatio-temporal patterns of skeletal movements and how to learn their discriminative features for classification tasks. This paper presents a novel skeleton-based representation and a deep learning framework for 3D action recognition using RGB-D sensors. We propose to build an action map called SPMF (<i>Skeleton Posture-Motion Feature</i>), which is a compact image representation built from skeleton poses and their motions. An Adaptive Histogram Equalization (AHE) algorithm is then applied on the SPMF to enhance their local patterns and form an enhanced action map, namely Enhanced-SPMF. For learning and classification tasks, we exploit Deep Convolutional Neural Networks based on the DenseNet architecture to learn directly an end-to-end mapping between input skeleton sequences and their action labels via the Enhanced-SPMFs. The proposed method is evaluated on four challenging benchmark datasets, including both individual actions, interactions, multiview and large-scale datasets. The experimental results demonstrate that the proposed method outperforms previous state-of-the-art approaches on all benchmark tasks, whilst requiring low computational time for training and inference.
Chondrogenesis and Mineralization During <i>In Vitro</i> Culture of Human Mesenchymal Stem Cells on Three-Dimensional Woven Scaffolds (2010)

Human mesenchymal stem cells (hMSCs) and three-dimensional (3D) woven poly(ɛ-caprolactone) (PCL) scaffolds are promising tools for skeletal tissue engineering. We hypothesized that in vitro culture duration and medium additives can individually and interactively influence the structure, composition, mechanical, and molecular properties of engineered tissues based on hMSCs and 3D poly(ɛ-caprolactone). Bone marrow hMSCs were suspended in collagen gel, seeded on scaffolds, and cultured for 1, 21, or 45 days under chondrogenic and/or osteogenic conditions. Structure, composition, biomechanics, and gene expression were analyzed. In chondrogenic medium, cartilaginous tissue formed by day 21, and hypertrophic mineralization was observed in the newly formed extracellular matrix at the interface with underlying scaffold by day 45. Glycosaminoglycan, hydroxyproline, and calcium contents, and alkaline phosphatase activity depended on culture duration and medium additives, with significant interactive effects (all p < 0.0001). The 45-day constructs exhibited mechanical properties on the order of magnitude of native articular cartilage (aggregate, Young's, and shear moduli of 0.15, 0.12, and 0.033 MPa, respectively). Gene expression was characteristic of chondrogenesis and endochondral bone formation, with sequential regulation of Sox-9, collagen type II, aggrecan, core binding factor alpha 1 (Cbfα1)/Runx2, bone sialoprotein, bone morphogenetic protein-2, and osteocalcin. In contrast, osteogenic medium produced limited osteogenesis. Long-term culture of hMSC on 3D scaffolds resulted in chondrogenesis and regional mineralization at the interface between soft, newly formed engineered cartilage, and stiffer underlying scaffold. These findings merit consideration when developing grafts for osteochondral defect repair.
Efficiency comparison of DC and AC microgrid (2015)

As the number of DC loads are growing in our daily uses, the low voltage DC (LVDC) distribution system is becoming important. Power supplied through the low voltage AC (LVAC) distribution system needs both the AC/DC rectifier and the DC/DC converter to supply the DC loads. In comparison, LVDC system would only need the DC/DC converter to supply the DC loads. Integration of renewable energy sources at LV level, like rooftop photovoltaic (PV), fuel cells, etc. would be relatively easier with the LVDC system than the LVAC. So, the LVDC can be the viable solution to reduce multiple conversion losses in the system. This paper presents comparison between the existing LVAC with the prospective LVDC distribution system in terms of converter conversion efficiency. Constant AC and DC loads, generally used in household applications, are simulated using PSCAD/EMTDC for both the LVAC and the LVDC distribution system. The analysis of the effectiveness of LVDC system over LVAC distribution system has been presented.
Kinetic-Based High-Throughput Screening Assay to Discover Novel Classes of Macrophage Migration Inhibitory Factor Inhibitors (2010)

Macrophage migration inhibitory factor (MIF) is a major mediator of innate immunity and inflammation and presents a potential therapeutic target for various inflammatory, infectious, and autoimmune diseases, including cancer. Although a number of inhibitors have been identified and designed based on the modification of known nonphysiological substrates, the lack of a suitable high-throughput assay has hindered the screening of chemical libraries and the discovery of more diverse inhibitors. Herein the authors report the development and optimization of a robust high-throughput kinetic-based activity assay for the identification of new MIF inhibitors. Using this assay, they screened 80,000 small molecules and identified and validated 13 novel inhibitors of MIF catalytic activity. These small molecules demonstrated inhibition constant (K(i,app)) values ranging from 0.5 to 13 microM.
Just blink your eyes (2003)

We propose a head-free, easy-setup gaze tracking system designed for a gaze-based Human-Computer Interaction. Our system enables the user to interact with the computer soon after catching the user's eye blinks. The user can move his/her head freely since the system keeps tracking the user's eye. In addition, our system only needs a 10 second calibration procedure at the very first time of use. An eye tracking method based on our unique eye blink detection and a sophisticated gaze estimation method using the geometrical eyeball model realize these advantages.
Safety of Human Embryonic Stem Cells in Patients with Terminal Conditions (2015)

Background: Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to self-renew and differentiate into all types of human cells. Purpose: The present study was aimed at establishing the safety of hESC therapy in patients with terminal/incurable conditions. Methods: This was a single cohort study conducted at Nutech Mediworld, New Delhi. The patients suffering from various degenerative diseases were included in the study from year 2002 to 2004. hESCs (0.25 mL) were injected under skin in the abdominal wall. The safety of hESC therapy was evaluated by assessing the AEs experienced by patients during the study. Any disabling symptom/ sign, teratoma or antigen-antibody reaction that a patient suffered post transplantation of hESCs was considered as an AE. Results: A total of four, six and twenty three patients received hESC therapy in the year 2002, 2003 and 2004 respectively. Pain and fever were the most common AEs observed during the study. Other AEs included headache, mild pain in the abdomen, swelling of legs (edema), urinary tract infection (UTI), rash/erythema, pain at the lower back and limbs and body ache. All the AEs reported were mild in nature and resolved within one or two days with symptomatic medication and rest. No serious AEs were reported. The improvement in specific parameters of the patients was observed after the therapy. Conclusion: hESCs used in the present study are safe for use in humans afflicted with incurable/terminal conditions. Future, prospective controlled studies to substantiate the present study are ongoing.
The Antimicrobial Compound Xantholysin Defines a New Group of Pseudomonas Cyclic Lipopeptides (2013)

The rhizosphere isolate Pseudomonas putida BW11M1 produces a mixture of cyclic lipopeptide congeners, designated xantholysins. Properties of the major compound xantholysin A, shared with several other Pseudomonas lipopeptides, include antifungal activity and toxicity to Gram-positive bacteria, a supportive role in biofilm formation, and facilitation of surface colonization through swarming. Atypical is the lipopeptide's capacity to inhibit some Gram-negative bacteria, including several xanthomonads. The lipotetradecadepsipeptides are assembled by XtlA, XtlB and XtlC, three co-linearly operating non-ribosomal peptide synthetases (NRPSs) displaying similarity in modular architecture with the entolysin-producing enzymes of the entomopathogenic Pseudomonas entomophila L48. A shifted serine-incorporating unit in the eight-module enzyme XtlB elongating the central peptide moiety not only generates an amino acid sequence differing at several equivalent positions from entolysin, but also directs xantholysin's macrocyclization into an octacyclic structure, distinct from the pentacyclic closure in entolysin. Relaxed fatty acid specificity during lipoinitiation by XtlA (acylation with 3-hydroxydodec-5-enoate instead of 3-hydroxydecanoate) and for incorporation of the ultimate amino acid by XtlC (valine instead of isoleucine) account for the production of the minor structural variants xantholysin C and B, respectively. Remarkably, the genetic backbones of the xantholysin and entolysin NRPS systems also bear pronounced phylogenetic similarity to those of the P. putida strains PCL1445 and RW10S2, albeit generating the seemingly structurally unrelated cyclic lipopeptides putisolvin (undecapeptide containing a cyclotetrapeptide) and WLIP (nonapeptide containing a cycloheptapeptide), respectively. This similarity includes the linked genes encoding the cognate LuxR-family regulator and tripartite export system components in addition to individual modules of the NRPS enzymes, and probably reflects a common evolutionary origin. Phylogenetic scrutiny of the modules used for selective amino acid activation by these synthetases indicates that bacteria such as pseudomonads recruit and reshuffle individual biosynthetic units and blocks thereof to engineer reorganized or novel NRPS assembly lines for diversified synthesis of lipopeptides.
Microwave-assisted Extraction of Phenolic Compounds from Grape Seed (2001)

A microwave-assisted extraction technique was developed to optimize the extraction of phenolic compounds from grape seeds. The microwave power (300-150W) and time of extraction (20-200s) were varied during the optimization process. The polyphenol content of the resulting extracts were measured as mg of tannic acid equivalent per gram of crude extract (mg TAE/g of crude extract), using a Folin-Ciocalteau reagent. In general, neither the time nor the power had a significant effect on the overall % yield (average of 13.5%) and on the polyphenol content (392 mg TAE/g of crude extract) of the extracts. However, when the solvent polarity was changed by the addition of 10% water, the yield increased to 15.2% and the polyphenol content increased to 429 mg TAE/g of crude extract.
Astrocytes contribute to Aβ‐induced blood–brain barrier damage through activation of endothelial <scp>MMP</scp>9 (2017)

The blood-brain barrier (BBB) plays an important role in the maintenance of the brain homeostasis, and its proper functions are warranted by the interplay between different cellular components (endothelial cells, astrocytes and pericytes). BBB dysfunctions in pathological conditions, and particularly in Alzheimer's disease, have been documented. Here, using an in vitroBBB model, the interaction between endothelial cells and astrocytes exposed to Aβ1-42 was investigated. Human endothelial cells, cultured in monolayer or co-cultured with astrocytes, were exposed to Aβ1-42 (2 μM for 18 h). Aβ induced dysfunction of endothelial barrier, as assessed by enhanced permeability to FITC-conjugated dextran and reduced expression of claudin-5; these modifications were observed in the co-culture model, but not in endothelial cells cultured in monolayer. Similarly, Aβ-induced damage at the barrier was observed when endothelial cells were challenged in the presence of conditioned medium generated by astrocytes previously exposed to Aβ (ACM Aβ). Endothelial barrier damages were associated with enhanced matrix metalloprotease 9 (MMP9) activity, known to mediate claudin-5 disruption. These events were not related to the direct effects played by Aβ on endothelial cells, but they were rather the consequence of Aβ-induced vascular endothelial growth factor (VEGF) expression in astrocytes. Indeed, when vascular endothelial growth factor expression was down-regulated in astrocytes, neither barrier properties or MMP9 expression in endothelial cells were affected after Aβ exposure both in the co-culture model or in the presence of ACM Aβ. These data point out the importance of astrocytes' mediation in inducing endothelial sensitivity to Aβ1-42<sub>.</sub>
Direct extraction of A and B blood group antigens from human red cells by liposomes (1996)

The A and B blood group antigens were directly extracted from red cells by liposomes without loss of antigenicity.
Integrated-optic acoustically tunable infra-red optical filter (1988)

We demonstrate a narrowband (≈1 nm) LiNbO3 acoustically tunable optical filter with a tuning range adequate to span the 1.3–1.55 μm wavelength range, exploiting the considerable advantages of an unconventional x-cut design.
RNA-dependent RNA polymerases of plants (1983)

The existence of RNA-dependent RNA polymerases (EC 2.7.7.48) in plants has been definitely proven by their isolation in pure form from cucumber and tobacco in our laboratory and from cowpea at Wageningen. These enzymes are single-chain proteins of 100-130 kilodaltons. They show clear physical and biochemical differences characteristic for a given plant species, even when their amounts in the plants were greatly increased prior to isolation by infection with the same virus. The role of these enzymes in plant physiology remains unknown.
Persistent Regional Downregulation in Mitochondrial Enzymes and Upregulation of Stress Proteins in Swine With Chronic Hibernating Myocardium (2007)

Hibernating myocardium is accompanied by a downregulation in energy utilization that prevents the immediate development of ischemia during stress at the expense of an attenuated level of regional contractile function. We used a discovery based proteomic approach to identify novel regional molecular adaptations responsible for this phenomenon in subendocardial samples from swine instrumented with a chronic LAD stenosis. After 3 months (n=8), hibernating myocardium was present as reflected by reduced resting LAD flow (0.75±0.14 versus 1.19±0.14 mL · min −1 · g −1 in remote) and wall thickening (1.93±0.46 mm versus 5.46±0.41 mm in remote, P <0.05). Regionally altered proteins were quantified with 2D Differential-in-Gel Electrophoresis (2D-DIGE) using normal myocardium as a reference with identification of candidates using MALDI-TOF mass spectrometry. Hibernating myocardium developed a significant downregulation of many mitochondrial proteins and an upregulation of stress proteins. Of particular note, the major entry points to oxidative metabolism (eg, pyruvate dehydrogenase complex and Acyl-CoA dehydrogenase) and enzymes involved in electron transport (eg, complexes I, III, and V) were reduced ( P <0.05). Multiple subunits within an enzyme complex frequently showed a concordant downregulation in abundance leading to an amplification of their cumulative effects on activity (eg, “total” LAD PDC activity was 21.9±3.1 versus 42.8±1.9 mU, P <0.05). After 5-months (n=10), changes in mitochondrial and stress proteins persisted whereas cytoskeletal proteins (eg, desmin and vimentin) normalized. These data indicate that the proteomic phenotype of hibernating myocardium is dynamic and has similarities to global changes in energy substrate metabolism and function in the advanced failing heart. These proteomic changes may limit oxidative injury and apoptosis and impact functional recovery after revascularization.
ChemInform Abstract: LACTONE SYNTHESIS VIA THE INTRAMOLECULAR ALKYLATION OF β‐KETO ESTER DIANIONS (1983)

Abstract Acetyl‐meldrumsäure (I) reagiert mit den Brom‐alkoholen (II) zu den Bromalkylacetessigsäureestern (III), die zu den makrocyclischen Estern (IV) cyclisiert werden.
Serializability in object-oriented database systems (2002)

In an object-oriented database the objects are encapsulated, that is, objects are only accessible by methods defined in the database system. The definition of object-oriented serializability takes advantage of the semantics and of the nesting of the methods. Therefore, a lower rate of conflicting accesses than with the conventional definition of serializability is achieved. Transactions of an object-oriented database are defined as open-nested transactions. Depending on the semantics of operations, actions can be serialized independently of the calling transactions. The techniques already used in multilayer transaction systems are extended to object-oriented systems. Object-oriented serializability includes multilayer serializability but allows a nonlayered, more general structure of the database system.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
JET NOISE REDUCTION TECHNOLOGY DEVELOPMENT AT GE AIRCRAFT ENGINES (2002)

Several years ago, GE Aircraft Engines realized that with the expected increased stringency of Stage 4 noise regulations, the continued thrust growth of engine families, and increasing environmental pressures, jet noise would become a restricting factor in aircraft operations. An internal research and development program, with some invaluable assistance from NASA, was started to investigate how to reduce jet noise while achieving acceptable impacts on performance, operability, manufacturability, weight, etc.… The chevron nozzle for separate flow exhaust systems was the outcome, reducing jet noise by enhancing mixing of the fan, core and ambient streams faster than conventional nozzles, with a minimal impact on performance. The chevron nozzle, consisting of cut-outs around the perimeter of the nozzle, generates stream-wise vorticity, enhancing the mixing between the two streams, reducing the peak velocity more quickly and therefore reducing peak noise. The physical blockage is very small with this concept, with relatively small impact on thrust and flow, attributed to the aerodynamic blockage of the streamwise vortices. This technology was developed in 1996, became part of a production exhaust system in 1999, and was first flown on an airplane in 2001. This paper provides a brief overview of this technology.
Gene Expression Profiling Identifies Molecular Subtypes of Inflammatory Breast Cancer (2005)

Breast cancer is a heterogeneous disease. Comprehensive gene expression profiles obtained using DNA microarrays have revealed previously indistinguishable subtypes of noninflammatory breast cancer (NIBC) related to different features of mammary epithelial biology and significantly associated with survival. Inflammatory breast cancer (IBC) is a rare, particular, and aggressive form of disease. Here we have investigated whether the five molecular subtypes described for NIBC (luminal A and B, basal, ERBB2 overexpressing, and normal breast-like) were also present in IBC. We monitored the RNA expression of approximately 8,000 genes in 83 breast tissue samples including 37 IBC, 44 NIBC, and 2 normal breast samples. Hierarchical clustering identified the five subtypes of breast cancer in both NIBC and IBC samples. These subtypes were highly similar to those defined in previous studies and associated with similar histoclinical features. The robustness of this classification was confirmed by the use of both alternative gene set and analysis method, and the results were corroborated at the protein level. Furthermore, we show that the differences in gene expression between NIBC and IBC and between IBC with and without pathologic complete response that we have recently reported persist in each subtype. Our results show that the expression signatures defining molecular subtypes of NIBC are also present in IBC. Obtained using different patient series and different microarray platforms, they reinforce confidence in the expression-based molecular taxonomy but also give evidence for its universality in breast cancer, independently of a specific clinical form.
Auditorio, en Israel (1962)

Haifa y, en el seno de ella, el Monte Carmelo, gozan de una situación privilegiada dentro de la topografía, en general seca y pedregosa de Israel.
Phosphatase activity of the histidine kinases ensures pathway specificity of the <scp>ChrSA</scp> and <scp>HrrSA</scp> two‐component systems in <scp><i>C</i></scp><i>orynebacterium glutamicum</i> (2014)

The majority of bacterial genomes encode a high number of two-component systems controlling gene expression in response to a variety of different stimuli. The Gram-positive soil bacterium Corynebacterium glutamicum contains two homologous two-component systems (TCS) involved in the haem-dependent regulation of gene expression. Whereas the HrrSA system is crucial for utilization of haem as an alternative iron source, ChrSA is required to cope with high toxic haem levels. In this study, we analysed the interaction of HrrSA and ChrSA in C. glutamicum. Growth of TCS mutant strains, in vitro phosphorylation assays and promoter assays of P(hrtBA) and P(hmuO) fused to eyfp revealed cross-talk between both systems. Our studies further indicated that both kinases exhibit a dual function as kinase and phosphatase. Mutation of the conserved glutamine residue in the putative phosphatase motif DxxxQ of HrrS and ChrS resulted in a significantly increased activity of their respective target promoters (P(hmuO) and P(hrtBA) respectively). Remarkably, phosphatase activity of both kinases was shown to be specific only for their cognate response regulators. Altogether our data suggest the phosphatase activity of HrrS and ChrS as key mechanism to ensure pathway specificity and insulation of these two homologous systems.