gebhart/patrepeval huggingface dataset:
A smart membrane based on an antigen‐responsive hydrogel (2006)

Hydrogel membranes have been fabricated that incorporate antibody/antigen moieties. The permeability of large solutes through these membranes is dependent on the presence of soluble antigen that can compete with the internal interactions between antibody and antigen leading to an increase in gel mesh size. Specifically, the membrane's structure is based on a dextran backbone grafted with a fluorescein isothiocyanate (FITC) antigen and a sheep anti-FITC IgG antibody. The backbone is covalently cross-linked by conjugated divinyl sulfone (DVS) groups. The gel structure is additionally stabilized by affinity crosslinks formed by biospecific interactions between the bound IgG and FITC. FTIR spectra of the gel are consistent with formation of covalent bonds between cysteine groups in the IgG and DVS groups in the dextran. Results obtained using isothermal titration calorimetry (ITC) confirmed the competitive interaction binding between IgG-FITC-dextran and free sodium fluorescein at pH 5.0. Scanning electron microscopy (SEM) of samples prepared using cryofixation and cryofracturing techniques showed that observed changes in permeability correlate with free fluorescein-dependent structural changes in the gel. Three-dimensional images obtained from confocal laser scanning microscopy show that these changes occur throughout the gel and indicate that SEM results are not artifacts of sample preparation. The permeability of these gels, as shown by blue-dextran (12 kDa) diffusion, increases in response to the presence of free fluorescein of the external medium, which causes competitive displacement of the affinity cross-links. Sequential addition and removal of sodium fluorescein showed that these permeability changes are reversible.
Effect of Different Distance Measures on the Performance of K-Means Algorithm: An Experimental Study in Matlab (2014)

K-means algorithm is a very popular clustering algorithm which is famous for its simplicity. Distance measure plays a very important rule on the performance of this algorithm. We have different distance measure techniques available. But choosing a proper technique for distance calculation is totally dependent on the type of the data that we are going to cluster. In this paper an experimental study is done in Matlab to cluster the iris and wine data sets with different distance measures and thereby observing the variation of the performances shown.
Mate quality affects offspring sex ratio in blue tits (1996)

Questions concerning the occurrence of adaptive sex ratio manipulation have a long research tradition in evolutionary biology, but evidence from birds is scarce. It has been suggested that birds are unable to adaptively determine the sex of their young because of the constraints of their sex determination systems. Here we report results from a study of variation in offspring sex ratio in the blue tit, Parus caeruleus, a small passerine bird. Our data suggest that females are able to manipulate their primary sex ratios away from the equal sex ratio predicted from simple chromosomal segregation at meiosis. Contrary to the situation in some other birds, offspring sex ratio variation in this species was not influenced by timing of breeding or brood size. We show that females mated to males with high survival prospects bias the hatching sex ratio of their broods in favour of sons. Although it has previously been proposed that females should produce an excess of sons when mated to high-quality males, our data provide the first evidence that the phenotypic quality of fathers contributes to the sex ratio variation in a natural population.
Quantitation of Serum Hemoglobin-Binding Capacity Using Cellulose Acetate Membrane Electrophoresis (1965)

Abstract A method is described for the quantitation of serum hemoglobin-binding capacity (haptoglobin level) using cellulose acetate membrane electrophoresis. Electrophoresis is carried out for 90 mm. Eight membranes are dried, stained, and scanned within 45 mm. The reproducibility of this technic is within ± 10%.
A Cognitive Model of Episodic Memory Integrated with a General Cognitive Architecture. (2004)

These results suggest that circuit resistance training augments plasma apelin and decreases both insulin and blood glucose. However, <i>Zataria multiflora</i> has no independent effect on apelin but does decrease blood glucose which is likely to be to some extent synergistic with training effects.
Effect of Oxyethylene Moieties in Hydrogenated Castor Oil on the Pharmacokinetics of Menatetrenone Incorporated in O/W Lipid Emulsions Prepared with Hydrogenated Castor Oil and Soybean Oil in Rats (2003)

Lipid emulsions with particle sizes of 190-270 nm were prepared with soybean oil (SO) and a series of hydrogenated castor oils (HCOs) with various oxyethylene numbers, and the effect of oxyethylene numbers of HCOs on the pharmacokinetics of menatetrenone incorporated into the lipid emulsions was studied in rats. Plasma half-life of menatetrenone after administration as the lipid emulsions prepared by HCO with 10 oxyethylene units (SO/HCO10) was similar to that after the administration as SO/egg yolk phosphatides (SO/EYP), but was shorter than that as the lipid emulsions prepared by HCOs with > 20 oxyethylene units (SO/HCO20, SO/HCO30, SO/HCO60, SOHC and SO/HCO100). Menatetrenone incorporated in SO/HCO10, SO/HCO20 and SO/HCO60 was not taken up by the blood cells in vitro, and the plasma level of menatetrenone incorporated in SO/HCO10 was similar to that of triglycerides, suggesting that menatetrenone was not released from the oil particles even after entering the circulation. Menatetrenone uptake by the liver for SO/HCO10 was similar to that for SO/EYP, while those for SO/HCO20, SO/HCO30, SO/HCO60 and SO/HCO100 was less than that for SO/EYP. These findings clearly demonstrate that 20 oxyethylene units in HCOs is the minimum requirement for the prolongation of the plasma circulation time of menatetrenone incorporated in SO/HCOs.
Automatic phase detection for stochastic on-chip traffic generation (2006)

During System on Chip (SoC) design, Network on Chip (NoC) prototyping is used for adapting NoC parameters to the application running on the chip. This prototyping is currently done using traffic generators which emulate the SoC components (IPs) behavior: processors, hardware accelerators, etc. Traffic generated by processor-like IPs is highly non-regular, it must be decomposed into program phases. We propose an original feature for NoC prototyping, inspired by techniques used in processor architecture performance evaluation: the automatic detection of traffic phases. Integrated in our NoC prototyping environment, this feature permits to have a completely automatic toolchain for the generation of stochastic traffic generators. We show that our traffic generators emulate precisely the behavior of processors and that our environment is a versatile tool for networks-on-chip prototyping. Simulations are performed in a SystemC-based simulation environment with a mesh network-on-chip (DSPIN) and a processor running MP3 decoding applications.
VLDL Hydrolysis by Hepatic Lipase Regulates PPARδ Transcriptional Responses (2011)

These data define a novel pathway involving HL hydrolysis of VLDL that activates PPARδ through generation of specific monounsaturated fatty acids. These data also demonstrate how integrating cell biology with metabolomic approaches provides insight into specific lipid mediators and pathways of lipid metabolism that regulate transcription.
High-Purity Preparation of HSV-2 Vaccine Candidate ACAM529 Is Immunogenic and Efficacious In Vivo (2013)

Genital herpes is a sexually transmitted infection (STI) caused by herpes simplex virus 2 (HSV-2) and to a lesser extent herpes simplex virus 1 (HSV-1). Infection by HSV-2 is life-long and is associated with significant cost to healthcare systems and social stigma despite the highly prevalent nature of the disease. For instance, the proportion of HSV-2 seropositive to seronegative adults is approximately 1 in 5 in the US and greater than 4 in 5 in some areas of sub-Saharan Africa. The replication-defective vaccine strain virus dl5-29 was re-derived using cells appropriate for GMP manufacturing and renamed ACAM529. Immunization with dl5-29 was previously reported to be protective both in mice and in guinea pigs, however these studies were performed with vaccine that was purified using methods that cannot be scaled for manufacturing of clinical material. Here we describe methods which serve as a major step towards preparation of ACAM529 which may be suitable for testing in humans. ACAM529 can be harvested from infected cell culture of the trans-complementing cell line AV529 clone 19 (AV529-19) without mechanical cell disruption. ACAM529 may then be purified with respect to host cell DNA and proteins by a novel purification scheme, which includes a combination of endonuclease treatment, depth filtration, anion-exchange chromatography and ultrafiltration/diafiltration (UF/DF). The resultant virus retains infectivity and is ∼ 200-fold more pure with respect to host cell DNA and proteins than is ACAM529 purified by ultracentrifugation. Additionally, we describe a side-by-side comparison of chromatography-purified ACAM529 with sucrose cushion-purified ACAM529, which shows that both preparations are equally immunogenic and protective when tested in vivo.
Inhibitory effect of soluble platelet‐derived growth factor receptor β on intraosseous growth of breast cancer cells in nude mice (2011)

Bone metastasis is a frequent complication of advanced breast cancer. On the basis of functional and molecular evidence, signaling mediated by the binding of platelet-derived growth factor (PDGF)-BB and -DD to PDGF receptor β (PDGFRβ) is critical for the survival and growth of metastatic breast cancer cells within the bone microenvironment. In this study, we propose a new approach to blocking PDGFRβ signaling using soluble PDGFRβ (sPDGFRβ) as a decoy receptor for PDGF-BB and -DD secreted from tumor cells and bone marrow stromal cells. A bone-seeking TNBCT/Bo cell line was established by in vivo selection from TNBCT human breast cancer cells, which are negative for estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 protein expression. The TNBCT/Bo cells were transfected with a mammalian expression vector encoding the extracellular domain of PDGFRβ. A stable transfectant (TNBCT/Bo-sPDGFRβ) grew at a similar rate to that of control cells under normal culture conditions, although growth stimulation of human fibroblasts with PDGF-BB was neutralized by the culture medium from TNBCT/Bo-sPDGFRβ cells. Intratibial injection of TNBCT/Bo-sPDGFRβ cells into athymic nude mice resulted in a significant decrease in tumor incidence compared with control mice (P < 0.01). This attenuated growth correlated with decreased cancer cell proliferation, angiogenesis, and recruitment of stromal cells, and with an increase in the number of apoptotic cells. These findings suggest that sPDGFRβ is useful for the treatment of breast cancer bone metastasis.
Axon deviation in the human lamina cribrosa (1998)

It is postulated that these axons would be selectively vulnerable to compression of the lamina cribrosa in diseases such as glaucoma in which the intraocular pressure is increased.
Unraveling the Chronic Toxicity of Lead: An Essential Priority for Environmental Health (1996)

Andrew C. Todd, James G. Wetmur, Jacqueline M. Moline, James H. Godbold, Stephen M. Levin, Philip J. Landrigan, Unraveling the Chronic Toxicity of Lead: An Essential Priority for Environmental Health, Environmental Health Perspectives, Vol. 104, Supplement 1 (Mar., 1996), pp. 141-146
Cognitive approach to a human adaptive robot development (2006)

A robotic-aid system could be more effective if the system were intelligent enough to understand human needs and adapt its behaviors accordingly. This paper presents our efforts to realize such a robot that we call a cognitive robot through a multiagent-based cognitive robot architecture with three distinctive memory systems. Several applications illustrate how the system interacts with humans.
Burden of first-ever ischemic stroke in the oldest old (2004)

The burden of ischemic stroke is high in subjects 80 years old or older, contributing about one-third of health care utilization and 59.8% of deaths within 30 days.
Automatic composer for forming rhythm patterns and entire musical pieces (1992)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Junichi Minamitaka; Automatic composer for forming rhythm patterns and entire musical pieces. J. Acoust. Soc. Am. 1 October 1992; 92 (4): 2287. https://doi.org/10.1121/1.405183 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAcoustical Society of AmericaThe Journal of the Acoustical Society of America Search Advanced Search |Citation Search
Adaptive array antennas for 5.8 GHz vehicle to roadside communication (2002)

In this paper a new concept for future short range vehicle to roadside communication systems at 5.8 GHz with electronically controlled array antennas is presented. The main beam of each antenna system is directed to fixed contours in the communication zone according to the vehicle's position. Interfering signals from vehicles on adjacent lanes are suppressed with an adaptive sidelobe reduction algorithm. Results for an automatic fee collection system show the efficiency of the adaptive array concept.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A monolithic glass chip for active single-cell sorting based on mechanical phenotyping (2014)

The mechanical properties of biological cells have long been considered as inherent markers of biological function and disease. However, the screening and active sorting of heterogeneous populations based on serial single-cell mechanical measurements has not been demonstrated. Here we present a novel monolithic glass chip for combined fluorescence detection and mechanical phenotyping using an optical stretcher. A new design and manufacturing process, involving the bonding of two asymmetrically etched glass plates, combines exact optical fiber alignment, low laser damage threshold and high imaging quality with the possibility of several microfluidic inlet and outlet channels. We show the utility of such a custom-built optical stretcher glass chip by measuring and sorting single cells in a heterogeneous population based on their different mechanical properties and verify sorting accuracy by simultaneous fluorescence detection. This offers new possibilities of exact characterization and sorting of small populations based on rheological properties for biological and biomedical applications.
Thickness dependent sensing mechanism in sorted semi-conducting single walled nanotube based sensors (2012)

Single walled carbon nanotube (SWCNT) networks present outstanding potential for the development of SWCNT-based gas sensors. Due to the complexity of the transport properties of this material, the physical mechanisms at stake during exposure to gas are still under debate. Previously suggested mechanisms are charge transfer between gas molecules and SWCNT and Schottky barrier modulation. By comparing electrical measurements with an analytical model based on Schottky barrier modulation, we demonstrate that one mechanism or the other is predominant depending on the percolation of metallic carbon nanotubes. Below the metallic SWCNT percolation threshold, sensing is dominated by the modulation of the Schottky barrier, while above this threshold, it is only attributed to a charge transfer between SWCNT and gas molecules. Both mechanisms are discussed in terms of sensitivity and resolution leading to routes for the optimization of a gas sensor architecture based on highly enriched semiconducting carbon nanotube films.
Binocular Rivalry and Stereoscopic Depth Perception (1962)

An investigation was made of stimulus factors causing retinal rivalry or allowing stereoscopic depth perception, given a requisite positional disparity. It is shown that similar colour information can be “filtered” out from both eyes; that stereopsis is not incompatible with rivalry and suppression of one aspect of the stimulus, and that the strongest cue for perception of stereoscopic depth is intensity difference at the boundaries of the figures in the same direction at each eye. Identity of colour can also act as a cue for stereopsis. The brightness of different monocular figures seen in the stereoscope in different combinations was estimated by a matching technique, and it is suggested that the perceived brightness is a compromise between the monocular brightness difference between figure and ground seen in relation to the binocular fused background, and the mean brightness of the figures. The results are discussed in terms of neurophysiological “on,” “off” and continuous response fibres.
Two-color laser-induced fluorescent thermometry for microfluidic systems (2008)

The feasibility of implementing a two-color laser-induced fluorescence (LIF) technique to study thermal transport at the microscale is investigated. Temperature-sensitive (Rhodamine B) and temperature-insensitive (Sulforhodamine-101) fluorescent dyes are used in tandem to determine fluid temperature with high accuracy and low noise using a pulsed Nd:YAG laser as an illumination source. While the fluorescence intensity of the temperature-sensitive dye is proportional to temperature, it is also biased by variations in the illuminating intensity. Therefore, a second temperature-insensitive dye is required to compensate for such biases. Calibration of the two-color LIF system using the RhB–SR101 dye combination in ethanol and water yields temperature sensitivities of −1.5% K−1 and −2.7% K−1, respectively, with volumetric illumination from an Nd:YAG laser. The feasibility of this methodology for conducting temperature measurements is explored by measuring a steady-state temperature gradient maintained across a microfluidic channel array by two large hot and cold reservoirs. These measurements reveal that the mean steady-state temperatures in the microchannels are within ±0.4 °C and ±0.3 °C of the predicted temperatures with ethanol and water as the solvents, respectively, with a spatial resolution of 22.2 × 22.2 µm. The experimental uncertainties in the measurements using the RhB–SR101 dye combination are ±0.48–0.59 °C and ±0.41–0.49 °C for ethanol and water, respectively.
Mechanism for Ordered Receptor Binding by Human Prolactin (2004)

Prolactin, a lactogenic hormone, binds to two prolactin receptors sequentially, the first receptor binding at site 1 of the hormone followed by the second receptor binding at site 2. We have investigated the mechanism by which human prolactin (hPRL) binds the extracellular domain of the human prolactin receptor (hPRLbp) using surface plasmon resonance (SPR) technology. We have covalently coupled hPRL to the SPR chip surface via coupling chemistries that reside in and block either site 1 or site 2. Equilibrium binding experiments using saturating hPRLbp concentrations show that site 2 receptor binding is dependent on site 1 receptor occupancy. In contrast, site 1 binding is independent of site 2 occupancy. Thus, sites 1 and 2 are functionally coupled, site 1 binding inducing the functional organization of site 2. Site 2 of hPRL does not have a measurable binding affinity prior to hPRLbp binding at site 1. After site 1 receptor binding, site 2 affinity is increased to values approaching that of site 1. Corruption of either site 1 or site 2 by mutagenesis is consistent with a functional coupling of sites 1 and 2. Fluorescence resonance energy transfer (FRET) experiments indicate that receptor binding at site 1 induces a conformation change in the hormone. These data support an "induced-fit" model for prolactin receptor binding where binding of the first receptor to hPRL induces a conformation change in the hormone creating the second receptor-binding site.
Repairing and Upgrading Your PC (2006)

Two hundred forty-two patients with primary hyperparathyroidism operated on at Akron City Hospital are reviewed. The importance of the association of peptic ulcer and pancreatitis with primary hyperparathyroidism is stressed. Nineteen percent of the patients had associated peptic ulcer or pancreatitis. The mechanisms involved in the production of these diseases in patients with primary hyperparathyroidism are emphasized. The two deaths occurred in the small but challenging group of patients with acute parathyroid crisis and carcinoma. The decision concerning the extent of parathyroidectomy should be made by the surgeon for each patient, based on the number, location and gross appearance of the identified glands. Removal of a single enlarged gland, if the other three glands are normal, is all that needs to be done in most cases. A recurrence rate of 1 percent and an appreciable decrease in postoperative tetany support this conservative approach. Subtotal parathyroidectomy should be reserved for patients with diffuse hyperplasia.
Simulated annealing statics computation using an order-based energy function (1991)

Abstract The residual statics problem in seismic data analysis is treated by introducing an optimization function that emphasizes the coherence of neighboring common depth point (CDP) gathers within a nonlinear simulated annealing technique. This optimization criterion contrasts with stack power optimization which only considers the coherence between traces within a single CDP. Emphasizing coherence between CDPs removes many of the phase space degeneracies that result from stack-power based optimization techniques. We have applied the method to both synthetic and real data sets, and initial results display significant improvement over the input data in the coherence of reflections, even in structurally complex areas.
STUDY ON THE DEMODULATION STRUCTURE OF READER RECEIVER IN A PASSIVE RFID ENVIRONMENT (2009)

In this paper, we present a demodulation structure suitable for a reader receiver in a passive Radio Frequency IDentification (RFID) environment. In a passive RFID configuration, undesirable DC-offset phenomenon may appear in the baseband of the reader receiver. As a result, this DC-offset phenomenon can severely degrade the performance of the extraction of valid information from a received signal in the reader receiver. To mitigate the DC-offset phenomenon, we propose a demodulation structure to reconstruct a corrupted signal with the DC-offset phenomenon, by extracting useful transition information from the corrupted signal. It is shown that the proposed method can successfully detect valid data from a received signal, even when the received baseband signal is distorted with the DC-offset phenomenon.