gebhart/patrepeval huggingface dataset:
A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo. (1988)

A monoclonal antibody that reacts with proteins in the nuclear pore complex of rat liver (Snow, C. M., A. Senior, and L. Gerace. 1987. J. Cell Biol. 104:1143-1156) has been shown to cross react with similar components in Xenopus oocytes, as determined by immunofluorescence microscopy and immunoblotting. We have microinjected the antibody into oocytes to study the possible role of these polypeptides in nucleocytoplasmic transport. The antibody inhibits import of a large nuclear protein, nucleoplasmin, in a time- and concentration-dependent manner. It also inhibits export of 5S ribosomal RNA and mature tRNA, but has no effect on transcription or intranuclear tRNA processing. The antibody does not affect the rate of diffusion into the nucleus of two small proteins, myoglobin and ovalbumin, indicating that antibody binding does not result in occlusion of the channel for diffusion. This suggests that inhibition of protein and RNA transport occurs by binding of the antibody at or near components of the pore that participate in mediated transport.
Stimulating Cardiac Muscle by Light (2011)

Our results demonstrate feasibility to control excitation and contraction in cardiac muscle by light, using the TCU approach. Optical pacing in this case uses less energy, offers superior spatiotemporal control and remote access and can serve not only as an elegant tool in arrhythmia research but may form the basis for a new generation of light-driven cardiac pacemakers and muscle actuators. The TCU strategy is extendable to (nonviral) stem cell therapy and is directly relevant to in vivo applications.
Myoglobin: The hydrogen atom of biology and a paradigm of complexity (2003)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Selective Hydrogenolysis of Glycerol to 1,3-Propanediol Catalyzed by Pt Nanoparticles–AlO<i>x</i>/WO3 (2012)

Abstract Tungsten oxide-supported platinum nanoparticles containing aluminum oxide species (Pt–AlOx/WO3) act as a highly efficient heterogeneous catalyst for the hydrogenolysis of glycerol to 1,3-propanediol without any additives. High 1,3-propanediol selectivity was observed due to the concerted effect of the Pt nanoparticles, aluminum oxide, and tungsten oxide support. The Pt–AlOx/WO3 catalyst is reusable with maintaining its high catalytic performance.
The Identity of Glutathione <i>S</i> -Transferase B with Ligandin, a Major Binding Protein of Liver (1974)

Evidence is presented that ligandin, an intracellular protein involved in the binding of such anions as bilirubin, indocyanine green, and penicillin, is identical to glutathione S -transferase B (EC 2.5.1.18), an enzyme catalyzing the conjugation of glutathione with such electrophiles as 1-chloro-2,4-dinitrobenzene, 1,2-dichloro-4-nitrobenzene, iodomethane, ethacrynic acid, and bromosulfophthalein. The proteins, isolated by distinct methods, have the same specificity for substrates and for ligands, react in identical fashion to antibody produced against ligandin, bear entirely similar physical characteristics and amino acid composition, and are both induced in response to phenobarbital. Indocyanine green, one of the ligands that is not effective as a substrate, was shown to competitively inhibit the conjugation reaction. It is suggested that specificity is directed toward compounds with electrophilic sites.
First report on natural Leishmania infection of Phlebotomus sergenti due Leishmania tropica by high resolution melting curve method in South-eastern Iran (2014)

This is the first report on P. sergenti as the main and proven vector of anthroponitic cutaneous leishmaniasis in Dehbakri County using Real-time PCR coupled with HRM analysis. This method is rapid, sensitive and specific for diagnosing of parasites in infected Sand flies and ideal for large scale genotyping projects.
Optical selection rules in superlattices in the envelope-function approximation (1984)

We show that the symmetry properties of the Bloch envelope functions in a superlattice influence considerably the absorption and emission line shapes associated with interband transitions. Absolute values of the absorption coefficients in superlattices are also discussed.
Epidemiology of visceral leishmaniasis in India. (1999)

Kala-azar has re-emerged from near eradication. The annual estimate for the incidence and prevalence of kala-azar cases worldwide is 0.5 million and 2.5 million, respectively. Of these, 90% of the confirmed cases occur in India, Nepal, Bangladesh and Sudan. In India, it is a serious problem in Bihar, West Bengal and eastern Uttar Pradesh where there is under-reporting of kala-azar and post kala-azar dermal leishmaniasis in women and children 0-9 years of age. Untreated cases of kala-azar are associated with up to 90% mortality, which with treatment reduces to 15% and is 3.4% even in specialized hospitals. It is also associated with up to 20% subclinical infection. Spraying of DDT helped control kala-azar; however, there are reports of the vector Phlebotomus argentipes developing resistance. Also lymphadenopathy, a major presenting feature in India raises the possibility of a new vector or a variant of the disease. The widespread co-existence of malaria and kala-azar in Bihar may lead to a difficulty in diagnosis and inappropriate treatment. In addition, reports of the organism developing resistance to sodium antimony gluconate--the main drug for treatment--would make its eradication difficult. Clinical trials in India have reported encouraging results with amphotericin B (recommended as a third-line drug by the National Malaria Eradication Programme). Phase III Trials with a first-generation vaccine (killed Leishmania organism mixed with a low concentration of BCG as an adjuvant) have also yielded promising results. Preliminary studies using autoclaved Leishmania major mixed with BCG have been successful in preventing infection with Leishmania donovani. Until a safe and effective vaccine is developed, a combination of sandfly control, detection and treatment of patients and prevention of drug resistance is the best approach for controlling kala-azar.
Validation and refinement of the Disease Risk Index for allogeneic stem cell transplantation (2014)

Because the outcome of allogeneic hematopoietic cell transplantation (HCT) is predominantly influenced by disease type and status, it is essential to be able to stratify patients undergoing HCT by disease risk. The Disease Risk Index (DRI) was developed for this purpose. In this study, we analyzed 13,131 patients reported to the Center for International Blood and Marrow Transplant Research who underwent HCT between 2008 and 2010. The DRI stratified patients into 4 groups with 2-year overall survival (OS) ranging from 64% to 24% and was the strongest prognostic factor, regardless of age, conditioning intensity, graft source, or donor type. A randomly selected training subgroup of 9849 patients was used to refine the DRI, using a multivariable regression model for OS. This refined DRI had improved prediction ability for the remaining 3282 patients compared with the original DRI or other existing schemes. This validated and refined DRI can be used as a 4- or 3-group index, depending on the size of the cohort under study, for prognostication; to facilitate the interpretation of single-center, multicenter, or registry studies; to adjust center outcome data; and to stratify patients entering clinical trials that enroll patients across disease categories.
PHASE TRANSFORMATIONS IN EMULSIONS : PART II : POLYMORPHISM FOR ORGANIC SUBSTANCES (1987)

ABSTRACT It is shown that the polymorphism of certain organic substances at at mospheric pressure depends strongly on the volume of the samples. As a general rule, we observe for these substances upon cooling of macrosam-3 pies (mm )3 the appearance of one or several crystalline stable phases, after small undercoolings. However with mlcrosamplea (μm3) made by emulsion techniques it appears, that after large undercoolings new crystalline phases are formed which can be preserved for a very long time although they are metastable. Hence, the polymorphism is changed and it is explained how it is possible to determine the nature, the temperatures and the enthalpies of the transformations between these crystalline phase (stable or metastable) or the liquid. The substances investigated are benzene derivatives, methylchloromethane compounds, cyclohexane, chloroform and liquid crystals. Consideration are given about the nucleation of metastable phases and crystal-crystal transformations in dispersed system.
Exploiting unlabeled data for improving accuracy of predictive data mining (2004)

Predictive data mining typically relies on labeled data without exploiting a much larger amount of available unlabeled data. We show that using unlabeled data can be beneficial in a range of important prediction problems and therefore should be an integral part of the learning process. Given an unlabeled dataset representative of the underlying distribution and a K-class labeled sample that might be biased, our approach is to learn K contrast classifiers each trained to discriminate a certain class of labeled data from the unlabeled population. We illustrate that contrast classifiers can be useful in one-class classification, outlier detection, density estimation, and learning from biased data. The advantages of the proposed approach are demonstrated by an extensive evaluation on synthetic data followed by real-life bioinformatics applications for (1) ranking PubMed articles by their relevance to protein disorder and (2) cost-effective enlargement of a disordered protein database.
Hot spot management with die-to-database wafer inspection system (2008)

We constructed hot spot management flow with a die-to-database inspection system that is required for both hot spot extraction accuracy and short development turn-around-time (TAT) in low k1 lithography. The die-to-database inspection system, NGR-2100, has remarkable features for the full chip inspection within reasonable operating time. The system provided higher hot spot extraction accuracy than the conventional optical inspection tool. Also, hot spots extracted by the system could cover all killer hot spots extracted by electrical and physical analysis. In addition, the new hot spot extraction methodology employing the die-to-database inspection system is highly advantageous in that it shortens development TAT by two to four months. In the application to 65nm node CMOS, we verified yield improvement with the new hot spot management flow. Also, the die-to-database inspection system demonstrated excellent interlayer hot spot extraction from the viewpoint of LSI fabrication.
A new water-soluble phosphine for use in aqueous organometallic systems. Products from the reactions of 2,3-bis(diphenylphosphino)maleic anhydride with water and oxygen (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA new water-soluble phosphine for use in aqueous organometallic systems. Products from the reactions of 2,3-bis(diphenylphosphino)maleic anhydride with water and oxygenAlfred Avey, David M. Schut, Timothy J. R. Weakley, and David R. TylerCite this: Inorg. Chem. 1993, 32, 2, 233–236Publication Date (Print):January 1, 1993Publication History Published online1 May 2002Published inissue 1 January 1993https://pubs.acs.org/doi/10.1021/ic00054a021https://doi.org/10.1021/ic00054a021research-articleACS PublicationsRequest reuse permissionsArticle Views298Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
GLI1 inactivation is associated with developmental phenotypes overlapping with Ellis–van Creveld syndrome (2017)

GLI1, GLI2 and GLI3 form a family of transcription factors which regulate development by mediating the action of Hedgehog (Hh) morphogens. Accordingly, inactivating variants in GLI2 and GLI3 are found in several developmental disorders. In contrast, loss-of-function mutations in GLI1 have remained elusive, maintaining enigmatic the role of this gene in the human embryo. We describe eight patients from three independent families having biallelic truncating variants in GLI1 and developmental defects overlapping with Ellis-van Creveld syndrome (EvC), a disease caused by diminished Hh signaling. Two families had mutations in the last exon of the gene and a third family was identified with an N-terminal stop gain variant predicted to be degraded by the NMD-pathway. Analysis of fibroblasts from one of the patients with homozygous C-terminal truncation of GLI1 demonstrated that the corresponding mutant GLI1 protein is fabricated by patient cells and becomes upregulated in response to Hh signaling. However, the transcriptional activity of the truncated GLI1 factor was found to be severely impaired by cell culture and in vivo assays, indicating that the balance between GLI repressors and activators is altered in affected subjects. Consistent with this, reduced expression of the GLI target PTCH1 was observed in patient fibroblasts after chemical induction of the Hh pathway. We conclude that GLI1 inactivation is associated with a phenotypic spectrum extending from isolated postaxial polydactyly to an EvC-like condition.
Immortalization of primary epithelial cells requires first- and second-exon functions of adenovirus type 5 12S (1992)

Immortalization of primary cells is a multistep process. The adenovirus E1A 12S gene product is a member of the class of oncoproteins that have the ability to establish primary cells as cell lines in culture. It is encoded by two exons. Extensive mutational analysis demonstrates that four regions of the E1A 12S gene, encoded by both exons, are necessary for immortalization of primary epithelial cells. Expression of two regions is necessary to activate quiescent cells into the cell cycle but is unable to extend the life span of these cells in culture and thus cannot immortalize them. These regions are encoded by the first exon. A third first-exon region, for which no function has yet been identified, is also required. These three regions are also required for 12S to cooperate with an activated ras gene to bring about tumorigenic transformation. The fourth region is required to maintain the cells in a proliferative mode, extend their life span in culture, and induce an autocrine growth factor. These functions are encoded by the second exon. The cells immortalized by wild-type 12S and immortalization-competent mutants retain their epithelial morphology and expression of keratin and vimentin intermediate filament proteins.
Efficient debugging primitives for multiprocessors (1989)

Existing kernel-level debugging primitives are inappropriate for instrumenting complex sequential or parallel programs. These functions incur a heavy overhead in their use of system calls and process switches. Context switches are used to alternately invoke the debugger and the target programs. System calls are used to communicate data between the target and debugger.
Three-dimensional culture of mouse pancreatic islet on a liver-derived perfusion-decellularized bioscaffold for potential clinical application (2015)

The cutting-edge technology of three-dimensional liver decellularized bioscaffold has a potential to provide a microenvironment that is suitable for the resident cells and even develop a new functional organ. Liver decellularized bioscaffold preserved the native extracellular matrix and three-dimensional architecture in support of the cell culture. The goal of this study was to discover if three-dimensional extracellular matrix derived from mouse liver could facilitate the growth and maintenance of physiological functions of mouse isolated islets. We generated a whole organ liver decellularized bioscaffold which could successfully preserve extracellular matrix proteins and the native vascular channels using 1% Triton X-100/0.1% ammonium protocol. To evaluate the potential of decellularized liver as a scaffold for islets transplantation, the liver decellularized bioscaffold was infused with mouse primary pancreatic islets which were obtained through Collagenase P digestion protocol. Its yield, morphology, and quality were estimated by microscopic analysis, dithizone staining, insulin immunofluorescence and glucose stimulation experiments. Comparing the three-dimensional culture in liver decellularized bioscaffold with the orthodoxy two-dimensional plate culture, hematoxylin-eosin staining, immunohistochemistry, and insulin gene expression were tested. Our results demonstrated that the liver decellularized bioscaffold could support cellular culture and maintenance of cell functions. In contrast with the conventional two-dimensional culture, three-dimensional culture system could give rise to an up-regulated insulin gene expression. These findings demonstrated that the liver bioscaffold by a perfusion-decellularized technique could serve as a platform to support the survival and function of the pancreatic islets in vitro. Meanwhile three-dimensional culture system had a superior role in contrast with the two-dimensional culture. This study advanced the field of regenerative medicine towards the development of a liver decellularized bioscaffold capable of forming a neo-organ and could be used as potential clinical application.
The Essential Oil of<i>Cordia cylindrostachya</i>Roem. & Schult. Grown on Aruba. (1990)

ABSTRACT Capillary GC of the essential oil of Cordia cylindrostachya Roem. & Schult. grown on Aruba showed a high content of tricyclene (ca. 58%), α-pinene (ca. 11%) and camphene (ca. 12%). The rest—about 20% of the total oil—consisted of 35 components, most of which were identified. Most of them occurred in amounts of less than 1%. The oil showed weak antibacterial and antifungal activities.
How to learn new and interesting things from model systems based on “exotic” biological species (2009)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Nanosecond time-resolved spectroscopy with a pulsed tunable dye laser and single photon time correlation (1977)

A technique is described for performing nanosecond time-resolved spectroscopy for situations of extremely low emission rates. The system utilizes a N(2) laser pumped tunable dye laser and single photon time correlation techniques. It is applicable where there is need for nanosecond time resolution on emission which is either long-lived or has extremely low quantum efficiency and especially where monochromatic excitation is required. The technique is applied to the relaxation between a pair of excited states of Tb(3+):Y(OH):(3), where 5-nsec temporal resolution with 2- cm(-1) spectral resolution is easily obtained.
Detection of elevated regions in surface images from laser beam melting processes (2015)

Laser Beam Melting (LBM) is a promising Additive Manufacturing technology that allows the layer-based production of complex metallic components suitable for industrial applications. Widespread application of LBM is hindered by a lack of quality management and process control. Elevated regions in produced layers pose a major risk to process stability as collisions between the powder coating mechanism and the part may occur, which cause damages to either one or even both. We train a classifier-based detector for elevated regions in laser exposure result images. For this purpose we acquire two high resolution layer images: one after laser exposure and another one after powder deposition for the next layer. Ground truth labels for critical regions are obtained from analysis of the latter, where elevated regions are not covered by powder. We compute dense descriptors (HOG, DAISY, LBP) on the surface image after laser exposure and compare their predictive power. The top five descriptor configurations are used to optimize parameters of Random Forest, Support Vector Machine and Stochastic Gradient Descent (SGD) classifiers. We validate the detectors with optimized parameters using cross-validation on 281 images from three build jobs. Using a DAISY descriptor with a SGD classifier we achieve a F <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</inf> -score of 0.670. The presented method enables detection of elevated regions before powder coating is performed and can be extended to other surface inspection tasks in LBM layer images. Detection results can be used to assess LBM process parameters with respect to process stability during process design and for quality management in production.
5'-adenosine monophosphate is the neutrophil-derived paracrine factor that elicits chloride secretion from T84 intestinal epithelial cell monolayers. (1993)

Neutrophil transmigration across intestinal epithelia is thought to contribute to epithelial dysfunction and characterizes many inflammatory intestinal diseases. Neutrophils activated by factors, normally present in the lumen, release a neutrophil-derived secretagogue activity to which intestinal epithelia respond with an electrogenic chloride secretion, the transport event which underlies secretory diarrhea. Using sequential ultrafiltration, column chromatographic, and mass and Raman spectroscopic techniques, neutrophil-derived secretagogue was identified as 5'-AMP. Additional studies suggested that neutrophil-derived 5'-AMP is subsequently converted to adenosine at the epithelial cell surface by ecto-5'-nucleotidase and that adenosine subsequently activates intestinal secretion through adenosine receptors on the apical membrane of target intestinal epithelial cells. These findings suggest that this ATP metabolite may serve as a neutrophil-derived paracrine mediator that contributes to secretory diarrhea in states of intestinal inflammation.