gebhart/patrepeval huggingface dataset:
<title>Laser Scanning In Microscopy</title> (1983)

Scanning techniques in optical microscopy up to now were employed in the rather limited field of microscope photometry. The use of lasers as a light source offers new possibilities because of the high intensity and good collimation of the laser beam. Modern electronic and scanning techniques make - in addition to the photometric possibilities - the build-up of high quality microscopic images possible.
Functional and Cooperative Interactions between the Homeodomain PDX1, Pbx, and Prep1 Factors on the Somatostatin Promoter (1999)

Expression of the somatostatin gene in endocrine pancreatic cells is controlled by several regulatory cis-elements located in the promoter region. Among these, the adjacent UE-A and TSEI elements, located from -113 to -85 relative to the transcription initiation site, function in combination and act as a pancreas-specific mini-enhancer. The TSEI element is recognized by the pancreatic homeodomain factor PDX1. In the present study, we show that the UE-A element binds a heterodimeric complex composed of a Pbx factor and the Prep1 protein, both belonging to the atypical three-amino acid loop extension homeodomain family. Recombinant Pbx1 and Prep1 proteins bind cooperatively to the UE-A site, whereas neither protein can bind this site alone. Transient transfection experiments reveal that both Pbx1 and Prep1 are required to generate a strong transcriptional activation from the UE-A element when this element is inserted close to the TATA box. In contrast, in the context of the intact somatostatin promoter or mini-enhancer, Pbx1 and Prep1 alone have no effect, but they produce a drastic activation when the pancreatic homeodomain factor PDX1 is also coexpressed. Thus, the activity of the somatostatin mini-enhancer is mediated by a cooperative interaction between the Pbx-Prep1 heterodimeric complex and the pancreatic factor PDX1.
Pure Polymorph C of Zeolite Beta Synthesized by Using Framework Isomorphous Substitution as a Structure-Directing Mechanism (2001)

Subsitution of germanium for silicon in the preparation of the zeolite Beta allows the synthesis and characterization of a third, heretofore predicted, polymorph "C"; a [001] view is shown in the picture. Germanium preferentially occupies positions in double four-membered ring cages, the secondary building units of polymorph C, and favors the formation of polymorph C regardless of which of the eight organic structure-directing agents were used.
Transplantation of human embryonic nerve tissue into a schizophrenic's brain. (1988)

Inspired by current knowledge of the possible participation of monoamines in the origin of schizophrenia and by experimental experience with embryonic nerve tissue transplantations, the authors ventured implantation of a human embryo's nervous tissue into a schizophrenic's brain. Six cubic millimeters of the transplant were placed in the septal area of both hemispheres. The substantial improvement of the clinical picture and, particularly, distinct improvement of memory abilities in the patient can more likely be attributed to the effect of the transplant rather than to the simultaneous mechanical intervention in the target structure of the recipient's brain.
Suppression of phosphatidylinositol 3-kinase/Akt signaling pathway is a determinant of the sensitivity to a novel histone deacetylase inhibitor, FK228, in lung adenocarcinoma cells (2005)

A novel histone deacetylase inhibitor, FK228, is a promising anticancer agent and has been proposed to modulate intracellular signaling, in addition to regulating gene transcription. We evaluated the effect of this agent on Akt-mediated signaling in relation to its cytotoxic activity using lung adenocarcinoma cell lines. Based on MTT assay and the appearance of cleaved poly (ADP-ribose) polymerase (PARP), we regarded A549 and PC14 cells as relatively sensitive and resistant cell lines, respectively. In A549 cells, FK228 suppressed the phosphorylation of Akt at Ser-473 and glycogen synthase kinase-3 without affecting these protein levels, indicating inhibition of the Akt-mediated signaling pathway. On the other hand, in PC14 cells, these biochemical reactions were not detected after treatment with FK228. The combination of FK228 and a phosphatidylinositol 3-kinase (PI3K)/Akt pathway inhibitor, LY294002, was determined to be synergistically cytotoxic in PC14 cells by isobologram analysis. This synergistic effect was attributable to the enhancement of apoptosis, as judged by flow cytometric analysis, and the appearance of cleaved PARP. The combination of FK228 with UCN-01, another PI3K/Akt pathway inhibitor, also exerted a synergistic effect. We concluded that FK228 suppresses the PI3K/Akt signaling pathway in a cell-specific manner, and this effect is a determinant of sensitivity to FK228.
Synthesis of dioxobis(tert-alkylimido)osmium(VIII) and oxotris(tert-alkylimido)osmium(VIII) complexes. Stereospecific vicinal diamination of olefins (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of dioxobis(tert-alkylimido)osmium(VIII) and oxotris(tert-alkylimido)osmium(VIII) complexes. Stereospecific vicinal diamination of olefinsAnthony O. Chong, Koichiro Oshima, and K. Barry SharplessCite this: J. Am. Chem. Soc. 1977, 99, 10, 3420–3426Publication Date (Print):May 1, 1977Publication History Published online1 May 2002Published inissue 1 May 1977https://doi.org/10.1021/ja00452a039RIGHTS & PERMISSIONSArticle Views1141Altmetric-Citations172LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (881 KB) Get e-Alerts Get e-Alerts
Chemical Characterization of Catalysts. II. Oxygen Exchange between Water and Cracking Catalysts (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemical Characterization of Catalysts. II. Oxygen Exchange between Water and Cracking CatalystsG. A. Mills and S. G. HindinCite this: J. Am. Chem. Soc. 1950, 72, 12, 5549–5554Publication Date (Print):December 1, 1950Publication History Published online1 May 2002Published inissue 1 December 1950https://pubs.acs.org/doi/10.1021/ja01168a051https://doi.org/10.1021/ja01168a051research-articleACS PublicationsRequest reuse permissionsArticle Views94Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Continuous Removal of Protein Aggregates by Annular Chromatography (2001)

The removal of polymeric proteins from their monomers is a frequently encountered separation task, especially in the polishing step of therapeutic proteins. Continuous separation of protein polymers from monomers by annular chromatography using size exclusion chromatography has been studied regarding the resolution, recovery, fouling, and productivity and has been compared to conventional chromatography. An IgG preparation rich in aggregates was used as a model protein mixture. Under conditions that maximized the throughput, the polymers could be separated from the monomers, but baseline separation could not be achieved. Baseline separation was also not possible in batch mode using equivalent conditions, which was also confirmed by computer simulation. For separation of the aggregates from the product the entire available separation space (360°) was indispensable. Therefore only cyclic, discontinuous regeneration could be carried out. Loading was identified as a critical step, since the concentrated protein solution evaded into the headspace instead of migrating into the gel where viscous fingering often occurs in conventional chromatography. The productivity of annular chromatography was two times higher than that of the conventional batch chromatography, and the buffer consumption was reduced to half the conventional value. These two benefits are especially important for protein separation processes that suffer from low loadability, such as size exclusion chromatography. We have demonstrated that size exclusion can be performed on an industrial scale when it is run continuously with the aid of a pressurized annular chromatograph.
System Health Monitoring for Space Mission Operations (2008)

Many spacecraft provide an abundance of system status telemetry that is monitored in real time by ground personnel and archived to allow for further analysis. In the flight control room, controllers typically monitor these values using text or graphical displays that incorporate individual parameter limit checking or simple trend analysis. Recent developments in data mining techniques for anomaly detection make it possible to use the wealth of archived system data to produce more sophisticated system health monitoring applications. These "data driven" applications are capable of characterizing and monitoring interactions between multiple parameters and can complement existing practice to provide valuable decision support for mission controllers. Data driven software tools have been successfully applied to mission operations for both the Space Shuttle and the International Space Station. These tools have been applied to engineering analysis of spacecraft data to detect unusual events in the data, and to real-time system health monitoring in the flight control room. Augmenting traditional mission control software with advanced monitoring tools can provide controllers with greater insight into the health and performance of the space systems under their watch. Adding heuristic rule based methods that encode system knowledge obtained from seasoned mission controllers can also be helpful to less experienced personnel. We will describe how such techniques have been applied to NASA mission control operations and discuss plans for future mission control system health monitoring software systems.
Elastomeric microposts integrated into microfluidics for flow-mediated endothelial mechanotransduction analysis (2012)

Mechanotransduction is known as the cellular mechanism converting insoluble biophysical signals in the local cellular microenvironment (e.g. matrix rigidity, external mechanical forces, and fluid shear) into intracellular signalling to regulate cellular behaviours. While microfluidic technologies support a precise and independent control of soluble factors in the cellular microenvironment (e.g. growth factors, nutrients, and dissolved gases), the regulation of insoluble biophysical signals in microfluidics, especially matrix rigidity and adhesive pattern, has not yet been achieved. Here we reported an integrated soft lithography-compatible microfluidic methodology that could enable independent controls and modulations of fluid shear, substrate rigidity, and adhesive pattern in a microfluidic environment, by integrating micromolded elastomeric micropost arrays and microcontact printing with microfluidics. The geometry of the elastomeric micropost array could be regulated to mediate substrate rigidity and adhesive pattern, and further the elastomeric microposts could be utilized as force sensors to map live-cell subcellular contractile forces. To illustrate the general application of our methodology, we investigated the flow-mediated endothelial mechanotransduction process and examined specifically the involvement of subcellular contractile forces in the morphological realignment process of endothelial cells under a sustained directional fluid shear. Our results showed that the cytoskeletal contractile forces of endothelial cells were spatiotemporally regulated and coordinated to facilitate their morphology elongation process along the direction of flow. Together, our study provided an integrated microfluidic strategy to modulate the in vitro cellular microenvironment with both defined soluble and insoluble signals, and we demonstrated its application to investigate quantitatively the involvement of cytoskeletal contractile forces in the flow-mediated mechanotransduction process of endothelial cells.
Efflux Pump Inhibitors: A Novel Approach to Combat Efflux-Mediated Drug Resistance in Bacteria (2015)

The increasing multi-drug resistance has become a major threat to the public health. Overexpression of multidrug efflux pumps is one of the major mechanisms of drug resistance in bacteria. Since active efflux of antibacterial agents plays a significant role in mediating drug resistance in bacteria, the inhibition of efflux pumps appears to be a promising strategy to restore antibacterial potency. In recent years, in order to address this grave problem of multiple drug resistance mediated by efflux pump, a large number of efflux pump inhibitors have been discovered and tested, including natural products, antibiotics and synthetic molecules. This review mainly describes recent achievements in the search for new molecules that are able to inhibit efflux pumps in both Gram-positive and Gram-negative bacteria, in particular emphasis on natural and synthetic inhibitors of the NorA efflux pump in Staphylococcus aureus, MexAB-OprM efflux pump in Pseudomonas aeruginosa, and AcrAB-TolC efflux pump in Enterobacteriaceae, giving special attention to their mechanisms of action, structure-activity relationships and synergetic effect with clinically available antibiotics.
Phase-conjugate feedback into a continuous-wave ring dye laser (1985)

Optical feedback from a BaTiO(3) passive phase-conjugate reflector into an untuned cw ring dye laser can narrow the linewidth to 1.3 GHz. Self-scanning effects are also observed. The linewidth results are interpreted in terms of spatial hole burning and longitudinal-mode competition.
Extracting user behavior by web communities technology on global web logs (2004)

Abstract. A lot of work has been done on extracting the model of web user behavior. Most of them target server-side logs that cannot track user behavior outside of the server. Recently, a novel way has been developed to collect web browsing histories, using the same method for determining TV audience ratings; i.e., by collecting data from randomly selected users called panels. The logs collected from panels(called panel logs) cover an extremely broad URL-space, and it is difficult to capture the global behaviors of the users. Here we utilize mining results of web community to group those URLs into easily understandable topics. We
Basic fibroblast growth factor modulates insulin-like growth factor-I, its receptor, and its binding proteins in hypothalamic cell cultures. (1992)

Interactions between different growth factors may be important in the regulation of cell growth and differentiation in the nervous system. For instance, basic fibroblast growth factor (bFGF) regulates neuroblast division through a mechanism probably involving insulin-like growth factor-I (IGF-I). In this regard, we previously found that simultaneous addition of both factors produces an additive effect on survival and differentiation of hypothalamic neuronal and glial cells in culture. To further analyze these interactions, we explored the influence of bFGF on IGF-I, its membrane receptor, and its binding proteins in hypothalamic cells. We also tested the effects of IGF-I on its own receptor and binding proteins (IGFBPs) to determine the specificity of bFGF's actions. Treatment of neuronal and glial cultures with bFGF produced an increase in IGF-I receptors, without changing their affinity, together with an increase in the apparent M(r) of the receptor. On the other hand, IGF-I elicited a down-regulation of its own receptor in both neurons and glia, without modifying its affinity. Treatment with bFGF also produced a marked differential effect on the IGFBPs secreted by the cells. While IGFBP levels in neuronal cultures were greatly increased by bFGF, their production by glial cells was inhibited. On the other hand, IGF-I increased the amount of IGFBPs in both types of cells. Finally, addition of bFGF to the cultures elicited a dose-dependent increase in the release of IGF-I to the medium, but only a moderate increase in cellular IGF-I content, in both neurons and glia. We conclude that bFGF strongly modulates IGF-I, its receptors, and its binding proteins in the two major cell types of the hypothalamus. These findings reinforce the possibility that IGF-I and/or its receptors and binding proteins are involved in the trophic effects of bFGF on developing brain cells.
Immediate Neurocognitive Effects of Concussion (2002)

These findings are the first to demonstrate not only that a gradient of increasing concussion severity is represented by PTA and LOC but also that measurable neurocognitive abnormalities are evident immediately after injury without PTA or LOC.
Cycle d’Etudes Sociales Européen (1950)

G. J. Cycle d’Etudes Sociales Européen. In: Bulletin du Groupe d'études de psychologie de l'Université de Paris, 3e année n°4, 1950. pp. 86-87.
Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes (2010)

Within the mammalian ovary, oocytes remain arrested at G2 for several years. Then a peri-ovulatory hormonal cue triggers meiotic resumption by releasing an inhibitory phosphorylation on the kinase Cdk1. G2 arrest, however, also requires control in the concentrations of the Cdk1-binding partner cyclin B1, a process achieved by anaphase-promoting complex (APCCdh1) activity, which ubiquitylates and so targets cyclin B1 for degradation. Thus, APCCdh1 activity prevents precocious meiotic entry by promoting cyclin B1 degradation. However, it remains unresolved how cyclin B1 levels are suppressed sufficiently to maintain arrest but not so low that they make oocytes hormonally insensitive. Here, we examined spatial control of this process by determining the intracellular location of the proteins involved and using nuclear-targeted cyclin B1. We found that raising nuclear cyclin B1 concentrations, an event normally observed in the minutes before nuclear envelope breakdown, was a very effective method of inducing the G2/M transition. Oocytes expressed only the α-isoform of Cdh1, which was predominantly nuclear, as were Cdc27 and Psmd11, core components of the APC and the 26S proteasome, respectively. Furthermore, APCCdh1 activity appeared higher in the nucleus, as nuclear-targeted cyclin B1 was degraded at twice the rate of wild-type cyclin B1. We propose a simple spatial model of G2 arrest in which nuclear APCCdh1-proteasomal activity guards against any cyclin B1 accumulation mediated by nuclear import.
Mathematical nature of and a family of lower bounds for the success probability of unambiguous discrimination (2002)

Unambiguous discrimination is a strategy to the discrimination problem that identifies the state with certainty, leaving a possibility of undecidability. This paper points out that the optimal success probability of unambiguous discrimination is mathematically the well-known semidefinite programming problem. A family of lower bounds of the optimal success probability is also given.
RolX: Role Extraction and Mining in Large Networks (2011)

Given a graph, how can we automatically discover roles for nodes? Roles could be, eg., ’bridges’, or ’periphery-nodes’, etc. Roles are compact summaries of a node’s behavior that generalize across networks. They enable numerous novel and useful network mining tasks, such as sense-making, searching for similar nodes, and node classification. We propose RolX (Role eXtraction), a scalable (linear in the number of edges), unsupervised learning approach for automatically extracting roles from general network data. We demonstrate the effectiveness of RolX on several network mining tasks, from exploratory data analysis to network transfer learning. Moreover, we compare network role discovery with network community discovery. We highlight fundamental differences between the two (e.g., roles generalize across disconnected networks, communities do not).
Effects of Baicalein, Berberine, Curcumin and Hesperidin on Mucin Release from Airway Goblet Cells (2003)

Baicalein, berberine, curcumin and hesperidin are the major components derived from Scutellaria baicalensis, Coptis japonica, Curcuma longa and Poncirus trifoliata, respectively. These plants have been used for the treatment of diverse chronic inflammatory diseases including respiratory disease in oriental medicine and their respective major components were reported to have various biological effects including anti-inflammatory activity. In the present study, we investigated whether these four natural products affect mucin release from airway goblet cells and compared the possible activities of these agents with the inhibitory action on mucin release by PLL and the stimulatory action by ATP. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using 3H-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on 3H-mucin release. The results were as follows: (i) baicalein did not affect mucin release significantly; (ii) berberine, curcumin and hesperidin increased mucin release at the highest concentration (10 - 4 M); (iii) PLL inhibited and ATP increased mucin release. We conclude that berberine, curcumin and hesperidin can increase mucin release by directly acting on airway mucin-secreting cells and suggest that these agents be further studied for possible use as mild expectorants during the treatment of chronic airway diseases. Abbreviations. PLL:poly- L-lysine ATP:adenosine triphosphate HTSE:hamster tracheal surface epithelial DMSO:dimethylsulfoxide IL-12:interleukin-12 PBS:phosphate-buffered saline