gebhart/patrepeval huggingface dataset:
Clinical Scale Generation of Functional Human Natural Killer Cells From Umbilical Cord Blood CD34-Positive Cells for Immunotherapy. (2009)

Abstract Abstract 2666 Poster Board II-642 Immunotherapy based on natural killer (NK) cell infusions is a potential adjuvant treatment for many cancers. Such therapeutic application in humans requires large numbers of functional NK cells that have been isolated and expanded using clinical grade protocols. We established an extremely efficient cytokine-based culture system for ex vivo expansion of NK cells from hematopoietic stem and progenitor cells from umbilical cord blood (UCB). Systematic refinement of this two-step system using a novel clinical grade medium resulted in a therapeutically applicable cell culture protocol. The use of GBGM culture media in a clinical applicable protocol allows the ex vivo expansion and differentiation of CD34+ cells to more than 4-logs into CD56+CD3- NK cells with very high purity. These ex vivo-generated CD56+ cell products contain NK cell subsets expressing CD94/NKG2A and KIR as well express high levels of activating NKG2D and NCRs. Functional analysis showed that CD56+ cell products containing alloreactive NK cells efficiently kill myeloid leukemia and melanoma tumor cells as well as primary acute myeloid leukemia (AML) cells. We have currently translated the protocol to clinical scale production using a closed cell culture bioprocess. CD34+ selection from frozen UCB samples (n=9) using the CliniMACS device was optimized and the selection resulted in a CD34+ enrichment with a mean number of 2,2 ± 1,6 × 106 cells and a purity of 71 ± 14 % CD34+ cells. Validation experiments using the CliniMACS selected cells showed, that the generation of sufficient numbers of NK cells without contaminating T-cells or B-cells under a closed system process is feasible. The cell cultures using bags show a mean expansion of 1273 ± 506 fold (range 759–1770 fold, which generated 8,6×108 − 1,9×109 NK cells from cord blood-derived CD34+ cells within maximal 6 weeks of culture. The mean purity of the NK cell product was 71 ± 9 % (range 63–80%) of the total cells in the bag cultures. These NK cell products will be used for immunotherapy in elderly AML patients. This study is a phase I dose escalation study in a series of 12 AML patients who have successfully achieved CR (<5% blasts in the bone marrow) after standard intensive chemotherapy. Disclosures: Spanholtz: Glycostem Therapeutics: Employment. Tordoir:Glycostem Therapeutics: Employment.
Research Priorities for Robust and Beneficial Artificial Intelligence (2015)

Success in the quest for artificial intelligence has the potential to bring unprecedented benefits to humanity, and it is therefore worthwhile to investigate how to maximize these benefits while avoiding potential pitfalls. This article gives numerous examples (which should by no means be construed as an exhaustive list) of such worthwhile research aimed at ensuring that AI remains robust and beneficial.
Tracking Human Body Pose on a Learned Smooth Space (2005)

Particle filtering is a popular method used in systems for tracking human body pose in video. One key difficulty in using particle filtering is caused by the curse of dimensionality: generally a very large number of particles is required to adequately approximate the underlying pose distribution in a high-dimensional state space. Although the number of degrees of freedom in the human body is quite large, in reality, the subset of allowable configurations in state space is generally restricted by human biomechanics, and the trajectories in this allowable subspace tend to be smooth. Therefore, a framework is proposed to learn a low-dimensional representation of the high-dimensional human poses state space. This mapping can be learned using a Gaussian Process Latent Variable Model (GPLVM) framework. One important advantage of the GPLVM framework is that both the mapping to, and mapping from the embedded space are smooth; this facilitates sampling in the low-dimensional space, and samples generated in the low-dimensional embedded space are easily mapped back into the original highdimensional space. Moreover, human body poses that are similar in the original space tend to be mapped close to each other in the embedded space; this property can be exploited when sampling in the embedded space. The proposed framework is tested in tracking 2D human body pose using a Scaled Prismatic Model. Experiments on real life video sequences demonstrate the strength of the approach. In comparison with the Multiple Hypothesis Tracking and the standard Condensation algorithm, the proposed algorithm is able to maintain tracking reliably throughout the long test sequences. It also handles singularity and self occlusion robustly. 1.
A post-translational modification in the GGQ motif of RF2 from E.coli stimulates termination translation (2000)

A post-translational modification in the GGQ motif of RF2 from E.coli stimulates termination translation
Composite and shaped pulses for efficient and robust pumping of disconnected eigenstates in magnetic resonance (2014)

Hyperpolarization methods, which can enhance nuclear spin signals by orders of magnitude, open up important new opportunities in magnetic resonance. However, many of these applications are limited by spin lattice relaxation, which typically destroys the hyperpolarization in seconds. Significant lifetime enhancements have been found with "disconnected eigenstates" such as the singlet state between a pair of nearly equivalent spins, or the "singlet-singlet" state involving two pairs of chemically equivalent spins; the challenge is to populate these states (for example, from thermal equilibrium magnetization or hyperpolarization) and to later recall the population into observable signal. Existing methods for populating these states are limited by either excess energy dissipation or high sensitivity to inhomogeneities. Here we overcome the limitations by extending recent work using continuous-wave irradiation to include composite and adiabatic pulse excitations. Traditional composite and adiabatic pulses fail completely in this problem because the interactions driving the transitions are fundamentally different, but the new shapes we introduce can move population between accessible and disconnected eigenstates over a wide range of radio-frequency (RF) amplitudes and offsets while depositing insignificant amounts of power.
Substituent Effects on the Dissociation Constants of 1-Aryl-5-hydroxy-2-hydroxymethyl-4-pyridones (1983)

Abstract Six new 1-aryl-5-hydroxy-2-hydroxymethyl-4-pyridones were synthesized and their dissociation constants were measured. Hammett analysis of pKa values for proton gain and proton loss gave the following equations: pK1=2.74–0.34σ and pK2=8.80–0.36σ, respectively.
Analysis of performance impact caused by power supply noise in deep submicron devices (1999)

The paper addresses the problem of analyzing the performance degradation caused by noise in power supply lines for deep submicron CMOS devices. We first propose a statistical modeling technique for the power supply noise including inductive I noise and power net IR voltage drop. The model is then integrated with a statistical timing analysis framework to estimate the performance degradation caused by the power supply noise. Experimental results of our analysis framework, validated by HSPICE, for benchmark circuits implemented on both 0.25 , 2.5 V and 0.55 , 3.3 V technologies are presented and discussed. The results show that on average, with the consideration of this noise effect, the circuit critical path delays increase by 33% and 18%, respectively for circuits implemented on these two technologies.
Stress stimulates transepithelial macromolecular uptake in rat jejunum (1998)

Evidence suggests that stress may be a contributing factor in intestinal inflammatory disease; however, the involved mechanisms have not been elucidated. We previously reported that acute stress alters epithelial physiology of rat intestine. In this study, we documented stress-induced macromolecular transport across intestinal epithelium. After exposure of Wistar-Kyoto rats to acute restraint stress, transport of a model protein, horseradish peroxidase (HRP), was assessed in isolated segments of jejunum. The flux of intact HRP was significantly enhanced across intestine from stressed rats compared with controls. Electron microscopy revealed HRP-containing endosomes within enterocytes, goblet cells, and Paneth cells of stressed rats. The number and area of HRP endosomes within enterocytes were found to be significantly increased by stress. HRP was also visualized in paracellular spaces between adjacent epithelial cells only in intestine from stressed rats. Atropine treatment of rats prevented the stress-induced abnormalities of protein transport. Our results suggest that stress, via a mechanism that involves release of acetylcholine, causes epithelial dysfunction that includes enhanced uptake of macromolecular protein antigens. We speculate that immune reactions to such foreign proteins may initiate or exacerbate inflammation.
Algebraic Reconstruction Techniques for Tomographic Particle Image Velocimetry (2007)

Tomographic particle image velocimetry (Tomo-PIV) is a tech-nique for three-component three-dimensional (3C-3D) veloc-ity measurement based on the tomographic reconstruction of a volume intensity field from multiple two-dimensional projec-tion. As such the performance and accuracy of this technique is highly dependant on the algorithm used for reconstruction. This paper presents an evaluation of four different tomographic re-construction algorithms, namely multiplicative algebraic recon-struction techinique (MART); adaptive algebraic reconstruction technique (AART); improved iterative algorithm for sparse ob-ject reconstruction (IIASOR); and simultaneous iterative recon-struction technique (SIRT). Results indicate that the MART and AART algorithms provide considerably better particle field re-constructions for fewer iterations.
ESCI award lecture: regulation, function and biomarker potential of DNA methylation (2015)

Methylation of DNA and modifications of histones have emerged as intricately involved in gene regulation as they cross-talk and respond in multiple ways to the activity of transcription factors. Measuring these epigenome components has become a powerful tool to identify regulatory principles and biomarkers that predict cellular state during development or disease. Here, I will focus on DNA methylation as a reversible epigenetic modification of DNA that has been studied in great detail at the level of the genome. Recent advances in sequencing have identified unexpected dynamics of this modification, which are tightly linked to gene regulation. Understanding how DNA methylation patterns are read and how they contribute to regulation will be critical to interpret and utilize genomic maps of DNA methylation. As these patterns are dynamic during cellular differentiation and perturbed in disease, they present an opportunity to use DNA methylation as a biomarker.
Online monitoring of power transformers - system technology and data evaluation (1999)

During the last few pears, the electricity market has changed in many countries worldwide. The more competitive situation leads to a higher cost consciousness on the part of utilities. One aspect for saving costs is reducing effort required for maintenance of power network equipment. Power transformers are among the most expensive single elements of the high-voltage transmission system. Monitoring systems can help to decrease the transformer life cycle costs and to increase the high level of availability and reliability. In 1997 two large power transformers were equipped with the new Siemens monitoring system. The structure of the system as well as a first data evaluation are presented in this paper.
Novel Water Soluble Soya Oil Polymer from Oxidized Soya Oil Polymer and Diethanol Amine (2013)

A cold water soluble, biobased polymer derived from autoxidized soya oil has been described. Oxidized soya oil polymer was obtained by exposure to air under daylight at room temperature. The thickness of the soya oil at the beginning of the reaction and the oxidation time adversely influenced the molecular weight of the soya oil polymer obtained. The oxidized soya oil polymer obtained was then reacted with diethanol amine to obtain a hydroxylated soya oil polymer. The cold water soluble hydroxylated soya oil polymer was characterized by viscosity measurements, elemental analysis, FT-IR, 1H-NMR, 13C-NMR and cosy-NMR.
Demand Analyses for the United States of America (2013)

For the United States of America over the years 1929-41 I have carried out demand analyses on a more extended plan with the aid of the series provided in the Survey of Current Business. Table 4 (at end) sets out in nearly all cases a number of analyses using different combinations of determining variables. The reason for this is not that each analysis is believed to be of equal interest and reliability as an expression of the demand relationship, but simply that by studying a number of equations for each commodity we can trace the influence of adding (or leaving out) variables in making demand analyses. In view of the frequent use of the regression of the amount demanded on income alone or on income and a trend it will be of particular interest to see how these combinations of variables work out in relation to other possible sets.
<i>I</i> <i>n</i> <i>s</i> <i>i</i> <i>t</i> <i>u</i> laser diagnostic studies of plasma-generated particulate contamination (1989)

Laser light scattering measurements show that certain silicon etching plasmas produce a significant amount of in situ, particulate contamination. The particles are suspended at the sheath boundaries. Simultaneous measurement of plasma negative ions by the use of two-photon laser-induced fluorescence technique suggests that the particles are negatively charged and so are electrostatically trapped at the sheath boundaries. The parametric conditions for particle formation and growth in the plasma are identified. A mechanism for nucleation and growth is suggested involving formation of plasma negative ions from etch products, ion clustering with plasma species, and cluster growth into particles with electrostatic suspension and trapping. The particles drop onto the wafer when the rf is turned off. Implications for dry process technology are discussed.
Pesticide removal efficiencies of soybean oil refining processes (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPesticide removal efficiencies of soybean oil refining processesMakoto. Miyahara and Yukio. SaitoCite this: J. Agric. Food Chem. 1993, 41, 5, 731–734Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 May 1993https://pubs.acs.org/doi/10.1021/jf00029a010https://doi.org/10.1021/jf00029a010research-articleACS PublicationsRequest reuse permissionsArticle Views336Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
El locust T (1984)

Un modelo genetico natural para el estudio de la reproduccion y la diferenciacion...Las mutaciones a nivel del locus T determinan un cuadro de fenomenos tipicos, caracterizados-por abortos habituales, infertilidad masculina y variaciones en el tamano, o ausencia de la cola.DEFINICIONEl locus T es una region cromosomica ubicada a 14 unidades de recombinacion genetica, a la izquierda del complejo mayor de histocompatibilidad murino, hacia el lado del centromero del cromosona 17 (3) (ver fig. 1). Dicha region genetica codifica, normalmente, para proteinas de membrana importantes en la comunicacion intercelular, en el desarrollo embrionario, la espermatogenesis y en especial la diferenciacion'de la hoja germinativa ectodermica.Las mutaciones a nivel del locus T determinan un cuadro de fenomenos tipicos que se caracterizan por abortos habituales, infertilidad masculina y variaciones en el tamano, o ausencia de la cola (ver fig. 2).CONCLUSIONEl complejo T /t es, en estos momentos uno de los mejores modelos geneticos que explica las infertilidades masculinas (azoospermias) y femeninas (abortadoras habituales). Por esta razon varios grupos de investigadores han dedicado sus esfuerzos al esclarecimiento de estos fenomenos geneticos y embrionarios, que tienen una relacion evidente con los fenomenos de seleccion natural, e implican sofisticados mecanismos moleculares.Como se puede apreciar, los estudios sobre este modelo natural se encuentran muy avanzados en el raton, existiendo solo dos evidencias publicadas (8, 9) que demuestran tipos de herencia parecidos al locus T en el humano. Para los medicos veterinarios y los zootecnistas, es de gran importancia el conocimiento de este tipo de estudios para que traten de asociarlos con los frecuentes casos de fertilidad disminuida y de esterilidad de los animales domesticos.
A Covalent Linker Allows for Membrane Targeting of an Oxylipin Biosynthetic Complex (2008)

A naturally occurring bifunctional protein from Plexaura homomalla links sequential catalytic activities in an oxylipin biosynthetic pathway. The C-terminal lipoxygenase (LOX) portion of the molecule catalyzes the transformation of arachidonic acid (AA) to the corresponding 8 R-hydroperoxide, and the N-terminal allene oxide synthase (AOS) domain promotes the conversion of the hydroperoxide intermediate to the product allene oxide (AO). Small-angle X-ray scattering data indicate that in the absence of a covalent linkage the two catalytic domains that transform AA to AO associate to form a complex that recapitulates the structure of the bifunctional protein. The SAXS data also support a model for LOX and AOS domain orientation in the fusion protein inferred from a low-resolution crystal structure. However, results of membrane binding experiments indicate that covalent linkage of the domains is required for Ca (2+)-dependent membrane targeting of the sequential activities, despite the noncovalent domain association. Furthermore, membrane targeting is accompanied by a conformational change as monitored by specific proteolysis of the linker that joins the AOS and LOX domains. Our data are consistent with a model in which Ca (2+)-dependent membrane binding relieves the noncovalent interactions between the AOS and LOX domains and suggests that the C2-like domain of LOX mediates both protein-protein and protein-membrane interactions.
Effect of finite word length on the accuracy of digital filters--a review (1971)

The accuracy of a digital filter is limited by the finite word length used in its implementation. Techniques have been developed to analyze this problem. Good agreement between the theoretical and experimental results has been reported. This paper discusses some of these accuracy problems and reviews some of the approaches used in investigating them. The calculation of the statistical mean-squared error at the output of the filter is discussed in detail.
Alternating hemiplegia of childhood and a pathogenic variant of <i>ATP1A3</i>: a case report and pathophysiological considerations (2017)

We describe a case of a child suffering from alternating hemiplegia with a heterozygous p. E815K pathogenic variant of ATP1A3. The patient started to present abnormal eye movements in the first days of life, followed by the appearance at 2 months of dystonic episodes, and later on, by recurrent episodes of alternating hemiplegia more often on the right side. A severe epilepsy started at the age of 2 years with episodes of status epilepticus since the onset which frequently recurred, requiring admission to the intensive care unit. MRI showed bilateral mesial temporal sclerosis and a left-sided ischaemic lesion. Interictal EEG showed bilateral abnormalities, whereas postictal EEG after status epilepticus showed overt slowing on the left side, suggesting a predominant involvement of ictal activity of the left hemisphere. We hypothesize that in our patient, the left hemisphere might have been more prominently affected by the pathogenetic abnormalities underlying alternating hemiplegia of childhood, rendering it more prone to early ischaemic lesions and recurrent unilateral status epilepticus. We speculate whether alternating hemiplegia of childhood shares some common pathophysiological mechanisms with familial hemiplegic migraine that may be associated with a pathogenic variant of ATP1A2.
Study on a Novel Modeling Method for the Electromagnetic Flywheel Systems (2013)

This study proposes a new modeling method for nonlinear systems based on a genetic algorithm (GA). Through this method, the transfer functions of the above unidentified systems can be identified. This method would well help the nonlinear systems to find out their optimal solutions in controls. First, the physical input-output data pairs of the unidentified system should be collected. Next, the coefficients of the first second-order transfer function can be identified by GA. Last, based on the errors between the physical data pair and the first second-order transfer function; similarly the second second-order transfer functions can be identified by GA.So far as known, electromagnetic flywheel (EF) systems are with many uncertainties, such as nonlinear electromechanical coupling and electromagnetic saturation, etc. They are difficult to accurately modeling by traditional mathematic ways that restrict the control and application of them. In order to confirm the effectiveness and feasibility of this proposed method, an EF system is setup for this study. By assessing the results of calculations and experiments shows that the proposed method is possible to be applied for any nonlinear systems.
Lichen Metabolites. 1. Inhibitory Action Against Leukotriene B<sub>4</sub>Biosynthesis by a Non-Redox Mechanism (1999)

Of several lichen metabolites isolated from Parmelia nepalensis and Parmelia tinctorum, the didepsides atranorin (4) and diffractaic acid (5), as well as (+)-protolichesterinic acid (7), inhibited LTB4 biosynthesis in polymorphonuclear leukocytes. Ethyl hematommate (3) and (+)-usnic acid (1) were only weak inhibitors, while methyl beta-orcinolcarboxylate (2) and gyrophoric acid (6) were inactive at concentrations up to 60 microM. Redox properties of the compounds were evaluated in terms of inhibition of nonenzymatic lipid peroxidation in model membranes, reactivity against the stable free radical 2,2-diphenyl-1-picrylhydrazyl, and deoxyribose degradation as a measure of hydroxyl-radical generation. The results revealed that lichen metabolites neither acted as antioxidants against the peroxidation process in model membranes nor did they scavenge or produce free radicals, suggesting that the inhibitory effects on LTB4 biosynthesis was due to specific enzyme interaction rather than a nonspecific redox mechanism.