gebhart/patrepeval huggingface dataset:
Cloning and Expression of Fused Fc-binding Protein(SPA-SPG) and Its Application in Purification of IgG (2004)

Using a bioinformatical approach, a hybrid gene encoding the Fc binding domains of both SPA and SPG (1 269 bp) was designed and synthesized. The sequence was designed by substitution of high usage codons for low usage ones of Escherichia coli . A total of 64 oligonucleotides were assembled in a single step, in which T4 DNA ligase ligated DNA fragments from a pool of overlapping oligonucleotides. The synthetic gene was cloned to pSK vector. After sequencing and remending, the gene was expressed using the expression vector pET 28a c(+). A band of 47 ku was detected with Western blot analysis. The protein so called MproteinAG has been covalently conjugated to the carboxyl terminated magnetic particles. With the MproteinAG magnetic particles,IgG from blood serium of rabbit, pig,dog,mouse, human, macaque, rat, cat, sheep, cattle, horse can be purificated in one hour.
Mathematical Modeling of Emulsion Copolymerization of Acrylonitrile/Butadiene (1996)

A mathematical model was developed to simulate the emulsion copolymerization of acrylonitrile/butadiene, otherwise known as nitrile rubber. Our goal was to develop a practical tool to predict polymer production rate and product quality on an industrial scale. Model predictions include the polymerization rate, the polymer composition, the number- and weight-average molecular weights, branching averages, and the number and average size of the polymer latex particles. The prediction of conversion was compared to a limited amount of industrial pilot-plant data and was found to be adequate. The model is currently being applied in two ways. The first application involves the testing of nonlinear model-based control schemes for an industrial nitrile rubber reactor. The second application involves using the model as a storehouse of prior knowledge for the implementation of a Bayesian sequential design of emulsion terpolymerization experiments as part of a larger, systematic study of multicomponent polymerization kinetics.
Antioxidative activity of lactic acid bacteria in yogurt (2011)

The antioxidative effect of intact cells and cell-free extract of Lactobacillus casei subsp.casei SY13 and Lactobacillus delbrueckii subsp.bulgaricus LJJ, isolated from the traditional yogurt, was evaluated by various antioxidant assays.The results showed that two Lactobacillus strains had good antioxidant capacity, inhibiting the peroxidation of linoleic acid by 62.95% and 66.16%.The cell-free extract showed excellent scavenging superoxide anion and hydroxyl radicals activity.The intact cells on 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) radical scavenging ability and chelating ferrous ion capacity were superior to cell-free extract.The highest reducing activity was equivalent to 305 and 294 μM L-cysteine.This study suggests that two strains are high antioxidative bacterial strains.Antioxidative property of lactobacilli would be useful in the dairy manufacturing industry.They could beneficially affect the consumer by providing dietary source of antioxidants.
Loss of NKX3.1 Favors Vascular Endothelial Growth Factor-C Expression in Prostate Cancer (2008)

Decreased levels of the prostate-specific homeobox protein NKX3.1 are correlated with hormone-refractory and metastatic prostate cancer. Thus, it is compelling to define the NKX3.1-regulated genes that may be important for the progression of the advanced stage of the disease. In this study, we showed that vascular endothelial growth factor-C (VEGF-C) is one such target gene of NKX3.1. NKX3.1 inhibited VEGF-C expression in prostate cancer, and the loss of NKX3.1 led to increased VEGF-C expression. Histone deacetylase 1 acted as a corepressor of VEGF-C expression along with NKX3.1. Activated RalA acted in synergy with the loss of NKX3.1 for VEGF-C transcription. Patients with deletions at chromosome 8p21.1-p21.2 as a sole deletion developed lymph node metastasis. Interestingly, the higher expression of VEGF-C in prostate cancer is also correlated with lymph node metastasis. Therefore, regulation of VEGF-C expression by NKX3.1 provides a possible mechanism by which the loss of NKX3.1 protein level leads to lymphangiogenesis in the late stages of advanced prostate cancer.
PAC-Bayesian Bounds based on the Rényi Divergence (2016)

International audience
Immunogenicity and reactogenicity of a three-dose primary vaccination course with a combined diphtheria-tetanus-acellular pertussis-hepatitis B-inactivated polio-haemophilus influenzae type b vaccine coadministered with a meningococcal C conjugate vaccine. (2004)

Coadministration of the combined DTPa-HBV-IPV/Hib vaccine and the meningococcal C conjugate vaccine during the same vaccination visit was immunogenic and safe.
Circular polarization-splitting common-path interferometer based on a zero-twist liquid crystal display (2009)

It is present a unique compact optical circular-polarization-splitting common-path interferometer, described based on a zero-twist liquid crystal display (LCD) [1]. A blazed diffraction grating is encoded onto the LCD. The optical train produces two beams, a reference beam with a sense of circular polarized light and a diffracted one with the opposite sense of circular polarized light. Using a linear polarizer, these two beams form an interferogram that can be used to analyze optically active media. The detail of the light behaviour through the optical train is present using Jones matrix's.
USEFULNESS OF THE DIAGNOSTIC MARKERS FOR THE RESTORER GENE Rf1 IN INHERITANCE STUDIES AT SUNFLOWER (2013)

The present study was carried out with the objective to establish the usefulness of the marker\nCAPSH13 in marker-assisted selection for fertility restoration in segregating populations of sunflower. Through F2 and\nBC1 analysis, different restorer lines used in Republic of Moldova were characterized for Rf1 allelic status and their\nfertility restoration ability to be used in sunflower breeding
A structured approach to assembly language programming (1988)

A method is described for teaching structured programming techniques to students of assembly language programming. Structured programming, historically, has only been within the realm of high-level languages (Pascal, C, etc.), while a more loose approach-one lacking a formal syntax-has traditionally been applied to low-level programming in assembly language. Borrowing words and symbols from Pascal and C, a simple syntax was devised, called Pseudo Code, that uses three basic structures: linear, conditional, and loop. Upon learning that all the programs can be written using only these three structures, students become convinced of the reduced complexity brought by Pseudo Code. A method is adopted that proceeds from the problem definition to the assembly language program using Pseudo Code as an interim step. Using this method, students at the author's college have successfully developed software in assembly language that would have been too complex for them to attempt without coding their solutions in a structured form.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Correctness of a compiler for a Lisp subset (1972)

Excerpts of a proof of correctness of a running Lisp compiler for the PDP-10 computer are given. Included are the rationale for presenting this proof and a discussion of an actual extension of the proof to another compiler.
INDUCTION OF CHRONIC AUTOIMMUNE ARTHRITIS IN DBA/1 MICE BY ORAL ADMINISTRATION OF TYPE II COLLAGEN AND <i>ESCHERICHIA COLl LIPOPOLYSACCHARIDE</i> (1996)

We have developed a new model of autoimmune arthritis in DBA/1 mice by feeding chick type II collagen (CII) for 2-3 week intervals over a 15 week period. Clinically evident arthritis occurred in 8/10 mice receiving native CII (nCII; 100 micrograms/mouse) alone at 9-13 weeks. Arthritis was aggravated by the further ingestion of CII, while remission occurred after withdrawal of the CII. Heat-denatured CII (dCII; 200 micrograms/mouse) was also arthritogenic if co-administered with ovoinhibitor (OVI; 2 mg/mouse), a proteinase inhibitor. Co-oral administration of lipopolysaccharide (LPS; 10 micrograms/mouse) with CII enhanced the antibody production and T-cell responses to CII, and induced a more chronic arthritis that progressed spontaneously without further administration of CII or LPS. Long-term oral administration of LPS alone also induced a mild arthritis characterized by destruction of bone rather than cartilage. These observations suggest that abnormal gastrointestinal absorption of dietary mimic antigens and intestinal bacterial toxins can potentially disrupt self-tolerance mechanisms, thereby precipitating or exacerbating autoimmune disease in genetically susceptible individuals.
Surfaces for high heat dissipation with no Leidenfrost limit (2017)

Heat dissipation from hot surfaces through cooling droplets is limited by the Leidenfrost point (LFP), in which an insulating vapor film prevents direct contact between the cooling droplet and the hot surface. A range of approaches have been developed to raise this limit to higher temperatures, but the limit still exists. Recently, a surface architecture, decoupled hierarchical structure, was developed that allows the suppression of LFP completely. However, heat dissipation by the structure in the low superheat region was inferior to other surfaces and the structure required an extensive micro/nano fabrication procedure. Here, we present a metallic surface structure with no LFP and high heat dissipation capacity in all temperature ranges. The surface features the nucleate boiling phenomenon independent of the temperature with an approximate heat transfer coefficient of 20 kW m−2 K−1. This surface is developed in a one-step process with no micro/nano fabrication. We envision that this metallic surface provides a unique platform for high heat dissipation in power generation, photonics/electronics, and aviation systems.
Fifty years of progress in acoustic phonetics (2004)

Three events that occurred 50 or 60 years ago shaped the study of acoustic phonetics, and in the following few decades these events influenced research and applications in speech disorders, speech development, speech synthesis, speech recognition, and other subareas in speech communication. These events were: (1) the source-filter theory of speech production (Chiba and Kajiyama; Fant); (2) the development of the sound spectrograph and its interpretation (Potter, Kopp, and Green; Joos); and (3) the birth of research that related distinctive features to acoustic patterns (Jakobson, Fant, and Halle). Following these events there has been systematic exploration of the articulatory, acoustic, and perceptual bases of phonological categories, and some quantification of the sources of variability in the transformation of this phonological representation of speech into its acoustic manifestations. This effort has been enhanced by studies of how children acquire language in spite of this variability and by research on speech disorders. Gaps in our knowledge of this inherent variability in speech have limited the directions of applications such as synthesis and recognition of speech, and have led to the implementation of data-driven techniques rather than theoretical principles. Some examples of advances in our knowledge, and limitations of this knowledge, are reviewed.
Therapeutic Applications of Bilirubin and Biliverdin in Transplantation (2007)

Bilirubin is the end product of heme catabolism by heme oxygenases. The inducible form of these enzymes is heme oxygenase-1 (HO-1), which is the rate-limiting enzyme that can degrade heme into equimolar quantities of carbon monoxide (CO), biliverdin, and free iron. Biliverdin is very rapidly converted to bilirubin by the enzyme biliverdin reductase, and free iron upregulates the expression of ferritin. HO-1 is a ubiquitous stress protein and is induced in many cell types by various stimuli. Induced HO-1 exerts antiinflammatory effects and modulates apoptosis. Expression of HO-1 in vivo suppresses the inflammatory responses in endotoxic shock, hyperoxia, acute pleurisy, and organ transplantation, as well as ischemia-reperfusion injury, and thereby provides salutary effects in these conditions. Accumulating evidence indicates that biliverdin/bilirubin can mediate the protective effects of HO-1 in many disease models, such as IRI and organ transplantation, via its antiinflammatory, antiapoptotic, antiproliferative, and antioxidant properties, as well as its effects on the immune response. This review attempts to summarize these protective roles as well as the molecular mechanisms by which biliverdin/bilirubin benefit IRI and solid-organ transplantation, including chronic rejection, and islet transplantation.
Macroautophagy is dispensable for intracellular replication of <i>Legionella pneumophila</i> in <i>Dictyostelium discoideum</i> (2003)

The Gram-negative bacterium Legionella pneumophila is a facultative intracellular pathogen of free-living amoebae and mammalian phagocytes. L. pneumophila is engulfed in phagosomes that initially avoid fusion with lysosomes. The phagosome associates with endoplasmic reticulum (ER) and mitochondria and eventually resembles ER. The morphological similarity of the replication vacuole to autophagosomes, and enhanced bacterial replication in response to macroautophagy-inducing starvation, led to the hypothesis that L. pneumophila infection requires macroautophagy. As L. pneumophila replicates in Dictyostelium discoideum, and macroautophagy genes have been identified and mutated in D. discoideum, we have taken a genetic and cell biological approach to evaluate the relationship between host macroautophagy and intracellular replication of L. pneumophila. Mutation of the apg1, apg5, apg6, apg7 and apg8 genes produced typical macroautophagy defects, including reduced bulk protein degradation and cell viability during starvation. We show that L. pneumophila replicates normally in D. discoideum macroautophagy mutants and produces replication vacuoles that are morphologically indistinguishable from those in wild-type D. discoideum. Furthermore, a green fluorescent protein (GFP)-tagged marker of autophagosomes, Apg8, does not systematically co-localize with DsRed-labelled L. pneumophila. We conclude that macroautophagy is dispensable for L. pneumophila intracellular replication in D. discoideum.
Entry of animal viruses and macromolecules into cells (1994)

The entry of animal viruses into cells is mediated by conformational changes in certain virion-particle components. These changes are triggered by the binding of virions to receptors and are influenced by low pH during receptor-mediated endocytosis. These conformational alterations promote the interaction of some viral proteins with cellular membranes thereby leading to transient pore formation and the disruption of ionic and pH gradients. The entry of toxins that do not possess receptors on the cell surface is promoted during the translocation of the virus genome or the nucleocapsid to the cytoplasm. A model is now presented which indicates that efficient virus translocation through cellular membranes requires energy, that may be generated by a protonmotive force. The entry of some animal viruses, as promoted by low pH, should thus only take place when a pH gradient and/or a membrane potential exist, but will not take place if these are dissipated, even if virion particles are present in an acidic environment.
Risk of acute angle-closure glaucoma after diagnostic mydriasis in nonselected subjects: the Rotterdam Study. (1997)

In nonselected white subjects of 55 years of age or older, the 2% prevalence of narrow anterior chamber angles is similar to that in a mixed black and white population in the United States. According to our protocol, 3 in 10,000 subjects are likely to develop AACG after diagnostic mydriasis followed by miotic drops.
Anticorrosive Primers (1952)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnticorrosive PrimersAllen L. Alexander, Harold J. E. Segrave, Rodger Freriks, and J. E. CowlingCite this: Ind. Eng. Chem. 1952, 44, 10, 2409–2414Publication Date (Print):October 1, 1952Publication History Published online1 May 2002Published inissue 1 October 1952https://pubs.acs.org/doi/10.1021/ie50514a036https://doi.org/10.1021/ie50514a036research-articleACS PublicationsRequest reuse permissionsArticle Views71Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
On Method of Extracting Joint Points for Generating Function-Fonts of Multi-Symbols (1993)

In the previous research, the authors proposed a method to generate high quality function-fonts for brush-written characters automatically. This method extracts sharp edges of boundary lines, so-called joint points, and approximates the boundary line between each adjacent joint points using piecewise polynomials. As the next step, we applyed the previous method for printed characters and figures. We find that they have another rules of quality and that the previous method cannot be adopted to these fonts. This paper aims to make clear how joint points should be determined for many kinds of fonts and to present a multi-stage algorithm to extract them exactly. The performance of the present method is verified by some experiments.
The Evolution, Chemistry and Applications of Homogeneous Liquid Redox Sulfur Recovery Techniques (2001)

Abstract Hydrogen sulfide is a highly undesirable component of natural gas and other gaseous streams. Its removal from sour natural gas streams is necessary for health and safety reasons and to prevent corrosion of equipment during its transmission and distribution. Herein an overview of desulfurization processes mainly concerning liquid redox sulfur recovery (LRSR) techniques is given. Three successful commercial applications of LRSR processes are the Stretford process, the LO-CAT process and the SulFerox process. The first using V(V)/V(IV) redox couple has dominated the 1980's, but now stopped spreading because of the chemical degradation and environmental problems with vanadium. The iron based processes employing Fe(III)/Fe(II) chelate systems, particularly the Fe(III)/Fe(II)-NTA system, is gradually replacing V(V)/V(IV) systems. However, the degradation of the chelating ligand in Fe(III)/Fe(II)-NTA system results in high chemical costs, which obviously obstructs the use of this system in large installations for the removal of H2S by homogeneous catalytic oxidation. Although the rate of degradation can be slowed considerably by addition of Na2S2O3, which is known as an effective scavenger for the hydroxyl radical, it seems that the problem involving the degradation of NTA in-process is not resolved completely yet. The serious deficiency calls for research or selection of robust ligands. Therefore, Processes using of non-degrading ligands or regeneration facilitated by microorganisms, either with or without organic ligand have appeared, some of them have been applied on a pilot scale. Other processes. Such as the biological treatment without an auxiliary redox couple and the combined sulfur/hydrogen recovery, are now being developed. Key Words: hydrogen sulfidesulfursulfur recoverydesulfurization processStretford processLO-CAT processSulFerox processClaus processhomogeneous redoxFe(III)/(II) redox coupleV(V)/V(IV) redox couplenitrilotriacetic acidethylenedinitrilotetraacetic acid2,6-pyridinedicarboxylic acid2-carboxy-8-hydroxyquinoline2,6-pyridinediphosphonic acid
Thermo‐Switchable Materials Prepared Using the OEGMA‐Platform (2011)

Abstract We describe here the advantages of oligo(ethylene glycol)‐based (co)polymers for preparing thermoresponsive materials as diverse as polymer‐enzyme bio‐hybrids, injectable hydrogels, capsules for drug‐release, modified magnetic particles for in vivo utilization, cell‐culture substrates, antibacterial surfaces, or stationary phases for bioseparation. Oligo(ethylene glycol) methacrylates (OEGMAs) can be (co)polymerized using versatile and widely‐applicable methods of polymerization such as atom transfer radical polymerization (ATRP) of reversible addition‐fragmentation chain‐transfer (RAFT) polymerization. Thus, the molecular structure and therefore the stimuli‐responsive properties of these polymers can be precisely controlled. Moreover, these stimuli‐responsive macromolecules can be easily attached to–or directly grown from–organic, inorganic or biological materials. As a consequence, the OEGMA synthetic platform is today a popular option for materials design. The present research news summaries the progress of the last two years.
Effekte temporärer Konsumsteuersenkungen als antizyklische Fiskalmaßnahme (2012)

Seit der globalen Finanz- und Wirtschaftskrise wird die Frage, ob Regierungen antizyklische Fiskalpolitik betreiben sollten, in akademischen Kreisen wieder vermehrt diskutiert. Eine vielfach benutzte Maßnahme sind temporäre schuldenfinanzierte Konsumsteuersenkungen.
Monitoring the Photocleaving Dynamics of Colloidal MicroRNA-Functionalized Gold Nanoparticles Using Second Harmonic Generation (2015)

Photoactivated drug delivery systems using gold nanoparticles provide the promise of spatiotemporal control of delivery that is crucial for applications ranging from regenerative medicine to cancer therapy. In this study, we use second harmonic generation (SHG) spectroscopy to monitor the light-activated controlled release of oligonucleotides from the surface of colloidal gold nanoparticles. MicroRNA is functionalized to spherical gold nanoparticles using a nitrobenzyl linker that undergoes photocleaving upon ultraviolet irradiation. The SHG signal generated from the colloidal nanoparticle sample is shown to be a sensitive probe for monitoring the photocleaving dynamics in real time. The photocleaving irradiation wavelength is scanned to show maximum efficiency on resonance at 365 nm, and the kinetics are investigated at varying irradiation powers to demonstrate that the nitrobenzyl photocleaving is a one-photon process. Additional characterization methods including electrophoretic mobility measurements, extinction spectroscopy, and fluorimetry are used to verify the SHG results, leading to a better understanding of the photocleaving dynamics for this model oligonucleotide therapeutic delivery system.
Delayed cerebral vasospasm is not reversible by aminophylline, nifedipine, or papaverine in a “two-hemorrhage” canine model (1983)

Angiographic spasm of cerebral arteries was produced in dogs by successive injections of cisternal blood 48 hours apart. Angiograms were taken before and after each cisternal injection. There was progressively greater angiographic vasospasm of the basilar artery. Intravenous aminophylline, 10 mg/kg/hr, reversed vessel constriction on the 1st and 3rd day after one injection of cisternal blood. On the 5th day after two blood injections (on Day 1 and Day 3), intravenous aminophylline, nifedipine (1 mg/kg), and intra-arterial bolus injection of 2 mg/kg papaverine failed to reverse the constriction. The intractable constriction produced in this model resembles that found in patients. The calcium antagonist, nifedipine, is as ineffective as the more traditional vasodilators in reversing vasospasm in this model.