gebhart/patrepeval huggingface dataset:
Gene Therapy: The Advent of Adenovirus (1994)

Adenovirus has been developed as an alternative gene transfer vehicle which has emerged as a potent vector with a promising future. Infection of cells with replication-incompetent adenoviruses allows the production of foreign proteins encoded by the engineered viruses without otherwise affecting the host cell. Of utmost relevance to gene therapy, postmitotic cells seem particularly adapted to gene transfer by adenovirus, since the viral genome is not integrated into the cell’s chromosome and presumably persists the life of the cell. Administration of recombinant adenoviruses directly in vivo opens the way to new therapeutic strategies. The first successful somatic gene therapy of a hepatic enzyme deficiency in an animal model was achieved using an adenovirus (Stratford-Perricaudet et al., 1990). Since this initial demonstration of the feasibility of correcting a genetic defect with adenovirus, many potential applications have been imagined. The field has aroused much enthousiasm as reflected at the December 1992 RAC meeting where approval was given to carry out clinical trials using three different recombinant adenoviruses capable of expressing the human CFTR, one of which was constructed in our laboratory (Rosenfeld et al., 1992).
Comparative Replication of <i>Lymantria dispar</i> Nuclear Polyhedrosis Virus Strains in Three Continuous-Culture Cell Lines (1989)

We compared the replication of the gypsy moth (Lymantria dispar) nuclear polyhedrosis virus in two new cell lines, from embryos and fat body of L. dispar, and in a previously available ovarian cell line. Three virus isolates (the Hamden strain [LDP-67] used commercially as GYPCHEK, a plaque-purified clone of Hamden [5-7d], and an isolate from Abington, Mass. [Ab]) were each tested on the three cell lines. The fat-body-derived cell line proved best in terms of occlusion body production for all three virus strains, with the highest yield produced by the Abington strain. On the basis of these results, we conclude that a more efficient in vitro production of gypsy moth virus can be obtained by using the fat body cell line in conjunction with the Abington strain of the virus.
The Great Powwow: The Story of the Nashaway Valley in King Philip's War (1934)

The Great Powwow: The Story of the Nashaway Valley in King Philip's War. By Clara Endicott Sears. (Boston: Houghton, Mifflin Company, 1934. xii + 288 pp. Illustrations. $2.00.) Get access Viola F. Barnes Viola F. Barnes Mt. Holyoke College Search for other works by this author on: Oxford Academic Google Scholar Journal of American History, Volume 21, Issue 2, September 1934, Pages 259–260, https://doi.org/10.2307/1896897 Published: 01 September 1934
Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA (2004)

By immunoaffinity column chromatography, we have purified two RNA polymerase complexes, the transcriptase and replicase, from vesicular stomatitis virus-infected baby hamster kidney cells. The transcriptase is a multiprotein complex, containing the virus-encoded RNA polymerase L and P proteins, and two cellular proteins, translation elongation factor-1alpha and heat-shock protein 60. In addition, the complex contains a submolar amount of cellular mRNA cap guanylyltransferase. The replicase, on the other hand, is a complex containing the viral proteins, L, P, and the nucleocapsid (N), but lacking elongation factor-1alpha, heat-shock protein 60, and guanylyltransferase. The transcriptase complex synthesizes capped mRNAs and initiates transcription at the first gene (N) start site, whereas the replicase complex initiates RNA synthesis at the precise 3' end of the genome RNA and synthesizes encapsidated replication products in the presence of the N-P complex. We propose that two RNA polymerase complexes that differ in their content of virally and host-encoded proteins are separately responsible for transcription and replication of vesicular stomatitis virus genome RNA.
Replay Remix (2009)

Research Article| July 01 2009 Replay Remix Perry Bard Perry Bard PERRY BARD is an artist living in New York whose work Man With A Movie Camera: The Global Remake was presented at Video Vortex. Search for other works by this author on: This Site PubMed Google Scholar Afterimage (2009) 37 (1): 2. https://doi.org/10.1525/aft.2009.37.1.2 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Perry Bard; Replay Remix. Afterimage 1 July 2009; 37 (1): 2. doi: https://doi.org/10.1525/aft.2009.37.1.2 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAfterimage Search This content is only available via PDF. © 2009 Afterimage/Visual Studies Workshop, unless otherwise noted. Reprints require written permission and acknowledgement of previous publication in Afterimage.2009 Article PDF first page preview Close Modal You do not currently have access to this content.
External noninvasive temporary cardiac pacing: clinical trials. (1985)

An external cardiac pacemaker-monitor has been developed that provides safe, effective noninvasive ventricular stimulation that is well tolerated in conscious patients and allows clear recognition of electrocardiographic response. The noninvasive temporary pacemaker (NTP) has now been applied in 134 patients in five hospitals. Stimulation was tolerated well in 73 of 82 conscious patients, and nine found it intolerable. The NTP was effective in evoking electrocardiographic responses in 105 patients; the 29 failures were in the presence of prolonged hypoxia or severe discomfort. The NTP was clinically useful in 82 patients: 43 of 86 were resuscitated from emergency or expected arrest, 38 of 40 were maintained in standby readiness for up to 1 month but did not require stimulation, and one of eight patients with tachycardia obtained some clinical benefit. The NTP was especially useful in 25 patients with complications or contraindications to endocardial pacing and in 57 patients in whom insertion of an endocardial electrode was avoided.
Game Changing Technology for Treating and Recycling Frac Water (2011)

Abstract This paper addresses an advanced oxidation and precipitation water treatment process employed as an on-the-fly fluid pretreatment during hydraulic fracturing operations. The water treatment technology will allow for substantial reuse of flowback and produced fluid while at the same time completely replacing liquid biocide and scale inhibitor fluid treatment during fracs. Additionally, the treatment process generates zero waste. To date, the technology has been used on hundreds of wells successfully treating over 17 million barrels. The paper will report on more than 2 years of field operations on hundreds of frac stimulations as well as numerous pilot operations in multiple shale plays. Dynamic tube-blocking tests show that the treated fluid will not deposit scale even after days of storage in an open frac tank. Field sample testing shows the injected brine has 3 to 6 log-cycle kill of sulfate-reducing and acid-producing bacteria populations. With the move toward environmentally safe chemicals, an economical process eliminating chemicals is a step forward for our industry. The equipment is purposely designed to segue directly into the fracturing process without interfering with service company pumping operations or having any compatibility problems with any service company products. Our paper will show definitive results from field operations of an economic water treatment system that will allow for a reduction in liquid chemical usage and closed-loop management of wastewater. The newest design would treat 80 barrels per minute, occupying a footprint roughly the size of a frac tank. The units can be deployed in tandem for higher flow rate requirements.
Metallothionein genes in the European flat oyster Ostrea edulis: a potential ecological tool for environmental monitoring? (2003)

are small essential proteins involved in cellular processes of metal handling and detoxification. Here, we describe the structure of 2 MT genes in a marine mollusc, the European flat oyster Ostrea edulis. The sequences of these 2 genes code for 2 proteins of 74 and 71 amino acids, respectively, with the 71 amino acid protein having an abnormal lack of cysteine residues. The direct quantification of metal ions bound to purified recombinant proteins demonstrated that the metal-binding capacity of the 2 proteins differs by about 20%. An enzyme-linked immunosorbent assay (ELISA) test, used to quantify MT protein expression in O. edulis exposed to metal under laboratory conditions, showed no significant induction of MTs, either in the gills or the digestive gland despite an increase in metal concentration observed in the same tissues. Similar results were observed with an MT-RNA expression study. These results seem to indicate a low involvement of MTs in metal detoxification in O. edulis. Examination of polymorphism in the coding sequence of OeMT (O. edulis metallothionein) genes by single-strand conformation polymorphism (SSCP) revealed a lack of genetic diversity in field populations.
Regional soiling stations for PV: Design, calibration and installation (2015)

Soiling rate (g/m2/day) and daily/annual soiling loss (%) of photovoltaic (PV) modules are primarily influenced by climatic condition of the site and tilt angle of PV modules. Therefore, for all the performance and energy prediction models used by the industry, accurate climate-specific and tilt-angle-specific soiling rate and daily/annual soiling loss data are needed. The primary focus of this work is to demonstrate the effectiveness of regional soiling stations designed to systematically collect this data and they are deployed in five regions in the United States. This paper presents information on the design, calibration and installation of soiling stations. Four of the sites are at the U.S. Department of Energy Regional Test Centers (RTC) in Albuquerque, NM; Golden, CO; Cocoa Beach, FL; and Essex Junction, VT, while a fifth site is in Mesa, AZ. Each soiling station is equipped with a data acquisition system and 10 laminated coupons tilted at angles of 0°, 5°, 10°, 15°, 20°, 25°, 30°, 35°, 40°. Each coupon contains two identical half-cells cut from a single monocrystalline silicon cell. In this test setup, the right half-cell is periodically cleaned and left half-cell is allowed to soil naturally so the above stated data can be collected throughout the year.
High-Power Machines and Starter-Generator Topologies for More Electric Aircraft: A Technology Outlook (2020)

More electric aircraft (MEA) architectures consist of several subsystems, which must all comply with the settled safety requirements of aerospace applications. Thus, achieving reliability and fault-tolerance represents the main cornerstone when classifying different solutions. Hybrid electric aircraft (HEA) extends the MEA concept by electrifying the propulsive power as well as the auxiliary power, and thereby pushing the limits of electrification. This paper gives an overview of the high-power electrical machine families and their associated power electronic converter (PEC) interfaces that are currently competing for aircraft power conversion systems. Various functionalities and starter-generator (S/G) solutions are also covered. In order to highlight the latest advancements, the efficiency of the world's most powerful aerospace generator (Mark 1) developed within the E-Fan X HEA project is graphically represented and assessed against other rivaling solutions. Motivated by the strict requirements on efficiency, power density, trustworthiness, as well as starting functionalities, supplementary considerations on the system-level design are paramount. In order to highlight the MEA goals and take advantage of all potential benefits, all subsystems must be treated as a whole. It is then shown that the combination of PECs, aircraft grid and electrical machines can be better adapted to benefit the overall system. This survey outlines the influence of these concerns and offers a view of the future technology outlook, as well as covering the present challenges and opportunities.
When Breast Cancer Recurs: A 3-Year Prospective Study of Psychological Morbidity (1996)

Abstract: In a prospective study of 269 women diagnosed with early breast cancer, 61 (23%) developed recurrence of their disease within the 3-year study period. Thirty-eight of these women were interviewed following this diagnosis. Women with recurrence of their breast cancer were significantly more likely to experience psychiatric morbidity than women who were disease-free (p < 0.001). There was no statistically significant difference in psychiatric disorder at the interview before recurrence occurred, nor in anxiety personality trait preoperatively between these two groups: mental state prior to the diagnosis of recurrence did not appear to predict which women would develop secondary disease. Fifty percent of the 38 women were found to be clinically anxious, depressed, or both at interview. This psychiatric distress was not associated with the side effects of current cancer treatments, nor with age, but appeared to be a direct response to the cancer having spread. Thirty of the 38 women did not receive any support, directly or by referral, from their hospital, following discovery of recurrent disease. There were some interesting differences in psychiatric morbidity between women, depending on how they interpreted information given to them about their prognosis. These findings have implications for the management of recurrent disease. Many patients in this distressing situation are in need of psychosocial support; doctors also need to receive appropriate training for conveying difficult information about both diagnosis and prognosis.?
Abstract 3224: Pre-clinical targeting of the metabolic phenotype of lymphoma by AZD3965, a selective inhibitor of monocarboxylate transporter 1 (MCT1) (2012)

Abstract Introduction - The metabolic phenotype of tumours (relative to most normal tissues) is shifted from oxidative phosphorylation to aerobic (Warburg Effect) or anaerobic glycolysis. This enables tumours to meet their energetic and biosynthetic demands even under conditions of low nutrient and O2. The end-product of glycolysis is lactate, a metabolic dead end, which if allowed to accumulate in the tumour cell may cause feedback inhibition of glycolysis and intra-cellular acidification resulting in inhibition of cell growth or survival. Experimental data - We have studied the effects of a potent, selective and orally available inhibitor of MCT1 in lymphoma cell lines and in vivo models. AZD3965 is a small molecule inhibitor of MCT1 with a binding affinity of 1.6 nM, is 6 fold selective over MCT2 and does not inhibit MCT4 at 10 μM. Both lactate transport and cell growth are potently inhibited by AZD3965 in lymphoma cell lines that preferentially express MCT1. Lactate transport inhibition in some cell lines also induces a cytotoxic effect. In vitro combination studies show that lactate transport inhibition can enhance the induction of cell death by doxorubicin. Blocking lactate transport in vitro also leads to a rapid inhibition of glucose uptake in the Raji Burkitt's lymphoma cell line. In vivo, AZD3965 is well tolerated and induces a dose and time dependent accumulation of lactate in the tumours, suppresses tumour growth and in the Raji model potentiates the effects of Rituxan, doxorubicin and bendamustine. Conclusions - Here we demonstrate that selective inhibition of lactate transport by the MCT1 inhibitor AZD3965 offers an novel mechanism for targeting the metabolic phenotype in tumours that preferentially express MCT1. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3224. doi:1538-7445.AM2012-3224
GlycoPep Detector: A Tool for Assigning Mass Spectrometry Data of N-Linked Glycopeptides on the Basis of Their Electron Transfer Dissociation Spectra (2013)

Electron transfer dissociation (ETD) is commonly used in fragmenting N-linked glycopeptides in their mass spectral analyses to complement collision-induced dissociation (CID) experiments. The glycan remains intact through ETD, while the peptide backbone is cleaved, providing the sequence of amino acids for a glycopeptide. Nonetheless, data analysis is a major bottleneck to high-throughput glycopeptide identification based on ETD data, due to the complexity and diversity of ETD mass spectra compared to CID counterparts. GlycoPep Detector (GPD) is a web-based tool to address this challenge. It filters out noise peaks that interfere with glycopeptide sequencing, correlates input glycopeptide compositions with the ETD spectra, and assigns a score for each candidate. By considering multiple ion series (c-, z-, and y-ions) and scoring them separately, the software gives more weighting to the ion series that matches peaks of high intensity in the spectra. This feature enables the correct glycopeptide to receive a high score while keeping scores of incorrect compositions low. GPD has been utilized to interpret data collected on six model glycoproteins (RNase B, avidin, fetuin, asialofetuin, transferrin, and AGP) as well as a clade C HIV envelope glycoprotein, C.97ZA012 gp140ΔCFI. In every assignment made by GPD, the correct glycopeptide composition earns a score that is about 2-fold higher than other incorrect glycopeptide candidates (decoys). The software can be accessed at http://glycopro.chem.ku.edu/ZZKHome.php .
Kimberlites of the Continental United States: A Review (1976)

Kimberlite, and rocks with kimberlitic affinities, have a relatively wide distribution throughout the United States. They occur in the western margin of the Appalachians from New York to Tennessee; in the central region of the U.S. including Kentucky, southern Illinois, Missouri, Kansas and Arkansas, and in the Western States of Montana, Colorado, Wyoming and the Colorado Plateau. However, diamond-bearing kimberlite is currently known to be present in only two areas, Arkansas and the Colorado-Wyoming State Line. The available data regarding the ages of intrusion for the various kimberlites indicate that activity of this type has occurred sporadically from at least Middle Paleozoic times to Mid-Tertiary. The spatial distribution of kimberlites within any area may be related to a local structural trend but the occurrence of kimberlites throughout the U.S. cannot at this time be reconciled to any continental structural pattern. In recent years geological interest in kimberlite has increased due to the mineralogical and geochemical investigations into the nature and origin of the lower crustal and upper mantle xenoliths that are often present in kimberlite. Examination of such xenoliths in the United States has resulted in "stratigraphic" sections through the crust and upper mantle to depths of approximately 200 km for specific areas.
Acoustic Field Analysis of Ultrasonic Focusing Transducer by Using Finite Element. Method and Hybrid Type Infinite Element Method (1995)

This paper presents the lousing characteristics and the time. response of ultrasonic focusing transducer which is a coupled system with an electromechanical and an acoustical component. The Finite Element Method and the Hybrid Type Infinite Element Method are applied for the analysis. The position of the focal points and the resolutions is obtained from the loosing characteristics and the time response. It is found that the transducer with the damper, which stabilizes the displacement of the radiation surface, gives a better resolution. In conclusion, the results could be applied to the design and the performance analysis of the ultrasonic focusing transducer.
다양화된 식생활을 위한 유리그릇 활성화방안 연구 (2017)

한국사회는 1970년대 이후 급격한 산업화와 함께 생활 소득이 크게 증대하면서 식생활에 큰 변화를 가져왔다. 쌀, 채소, 발효 음식을 중심으로 한 한식에 의존하던 식습관에서 벗어나 서구화된 음식이나 간편식이 일상화되었고 친환경적 소비와 생활에 대한 관심이 높아졌다. 이러한 경향은 도시화된 지역과 고학력, 젊은 층에서 더욱 높게 나타나고 있으며 지속적인...
Electrostatic Analyzer with Variable Focal Length (1961)

In the electrostatic analyzer of sector type with a given mean radius and a given openning angle, the focal length of the apparatus depends on the curvature of the central part of the equipotential line in the plane perpendicular to the beam. Therefore, an electrostatic analyzer with variable focal length may be proposed if the field shape near the central beam can be controled electrically without changing the geometry of the apparatus. In this paper, a method of such a control is proposed and the variation of the field shape due to this method is described. A small 90° electrostatic analyzer with 162.5 mm mean radius was constructed and the focusing properties of this apparatus investigated. It was possible to focus a sharp radial image and a sharp axial image on a fluorescent screen at any location by the electrical control only.
Electrically Driven Reversible Insulator–Metal Phase Transition in 1T-TaS<sub>2</sub> (2015)

In this work, we demonstrate abrupt, reversible switching of resistance in 1T-TaS2 using dc and pulsed sources, corresponding to an insulator-metal transition between the insulating Mott and equilibrium metallic states. This transition occurs at a constant critical resistivity of 7 mohm-cm regardless of temperature or bias conditions and the transition time is significantly smaller than abrupt transitions by avalanche breakdown in other small gap Mott insulating materials. Furthermore, this critical resistivity corresponds to a carrier density of 4.5 × 10(19) cm(-3), which compares well with the critical carrier density for the commensurate to nearly commensurate charge density wave transition. These results suggest that the transition is facilitated by a carrier driven collapse of the Mott gap in 1T-TaS2, which results in fast (3 ns) switching.
Evaluation of aerial thermal imaging for detecting white-tailed deer in a deciduous forest environment (2003)

Estimating abundance of free-ranging white-tailed deer (Odocoileus virginianus) with tra- ditional methods often produces variable results, is labor-intensive, requires specific weather conditions, and is expensive. Thermal imaging from an airborne platform prom- ises advantages over conventional techniques. We estimated variation in the detection rate of white-tailed deer in a deciduous forest landscape in central Missouri using aerial surveys (n= 10) with a state-of-the-art thermal imaging system. Replicated aerial mark-resight surveys (n=I 1) provided an independent abundance estimate for evaluat- ing thermal imaging estimates. Based on an aggregated mark-resight estimate of 311 deer, detection rates for thermal imaging surveys ranged from 31-89% (x=56%, SE=6.7). Variability in detection was attributed to inconsistent sensor operation and variable ther- mal contrast between deer and background objects. Operator bias likely will remain problematic until hardware and software developments can automate documentation of ground coverage. Further study is needed to identify factors impacting thermal contrast and detection rate. Until the capabilities of thermal imaging are more fully understood and the sampling protocols refined, detection rates may be too variable to provide reli- able counts of animal abundance.