gebhart/patrepeval huggingface dataset:
Fas/FasL interaction: A novel immune therapy approach with immobilized biologicals (2004)

Systemically applied agents to modulate the Fas/FasL system, e.g., by stimulation of Fas on activated leukocytes or tumor cells failed as strategies in immune therapy due to severe toxic effects in the host. Recently, a novel strategy has been developed by using immobilized immune active biologicals in a medical device that may allow immune management without expensive systemic therapy. This review reports on the potential role of Fas/FasL in immune therapy and summarizes current experimental and clinical data with the leukocyte inhibition module (LIM), an immobilized anti-Fas antibody containing device yet used in extracorporeal blood circulation. This proof of principal may stimulate the development of other devices based on the regulation of Fas/FasL or other targets relevant for immune disorders. © 2004 Wiley Periodicals, Inc.
Divalent Metal Ion Triggered Activity of a Synthetic Antimicrobial in Cardiolipin Membranes (2009)

One member of a prototypical class of antimicrobial oligomers was used to study pore formation in cardiolipin-rich membranes. Both vesicle dye-leakage assays and small-angle X-ray scattering were used to study bilayer remodeling. The results indicate that the presence of negative intrinsic curvature lipids is essential for pore formation by this class of molecules: In Gram-positive bacteria, cardiolipin and divalent metal cations like Ca(2+) and Mg(2+) are needed. This is consistent with the role of phosphatidylethanolamine (PE) lipid in Gram-negative bacteria, where antimicrobial activity is dependent on the negative intrinsic curvature of PE rather than a specific interaction with PE.
A unified multiple-level cache for high performance storage systems (2005)

Multi-level cache hierarchies are widely used in high-performance storage systems to improve I/O performance. However, traditional cache management algorithms are not suited well for such cache organizations. Recently proposed multi-level cache replacement algorithms using aggressive exclusive caching work well with single or multiple-client, low-correlated workloads, but suffer serious performance degradation with multiple-client, high-correlated workloads. In this paper, we propose a new cache management algorithm that handles multi-level buffer caches by forming a unified cache (uCache) which uses both exclusive caching in L2 storage caches and cooperative client caching. We also propose a new local replacement algorithm, frequency based eviction-reference (FBER), based on our study of access patterns in exclusive caches. Our simulation results show that uCache increases the cumulative cache hit ratio dramatically. Compared to other popular cache algorithms, like LRU, the I/O response time is improved by up to 46% for low-correlated workloads and 53% for high-correlated workloads.
Studies on Complexes. XIII. Effect of Complex Formation on Drug Absorption from Alimentary Tract. (4) (1968)

The effect of complex formation on drug absorption from rat small intestine by recirculating perfusion method has been studied by following systems : sodium p-aminosalicylate (NaPAS)-sulfisoxazole, NaPAS-sulfisomidine, NaPAS-sulfamethoxypyridazine, sodium salicylate-caffeine, hydroxyethyltheophylline-nicotinamide, and its reverse combination, and the absorption rate of the latter of the each combinations was measured in the presence of the former. It was found that the absorption rate of each drug could be modified by the complex formation. Results of these studies were correlated with kinetic analysis based on the absorption rate constant in the absence and in the presence of complexing agent, and the equilibrium constant. It appears under the experimental conditions and used combinations of drugs that the complex itself may be absorbed, and that its absorption rate is slower than that of free drug. Complexation of the drug with complexing one decreased the apparent partition coefficient of the former. This suggests one possible mechanism for the absorption of complex.
Cancer risk assessment, indicators, and guidelines for polycyclic aromatic hydrocarbons in the ambient air. (2002)

Polycyclic aromatic hydrocarbons (PAHs) are formed during incomplete combustion. Domestic wood burning and road traffic are the major sources of PAHs in Sweden. In Stockholm, the sum of 14 different PAHs is 100-200 ng/m(3) at the street-level site, the most abundant being phenanthrene. Benzo[a]pyrene (B[a]P) varies between 1 and 2 ng/m(3). Exposure to PAH-containing substances increases the risk of cancer in humans. The carcinogenicity of PAHs is associated with the complexity of the molecule, i.e., increasing number of benzenoid rings, and with metabolic activation to reactive diol epoxide intermediates and their subsequent covalent binding to critical targets in DNA. B[a]P is the main indicator of carcinogenic PAHs. Fluoranthene is an important volatile PAH because it occurs at high concentrations in ambient air and because it is an experimental carcinogen in certain test systems. Thus, fluoranthene is suggested as a complementary indicator to B[a]P. The most carcinogenic PAH identified, dibenzo[a,l]pyrene, is also suggested as an indicator, although it occurs at very low concentrations. Quantitative cancer risk estimates of PAHs as air pollutants are very uncertain because of the lack of useful, good-quality data. According to the World Health Organization Air Quality Guidelines for Europe, the unit risk is 9 X 10(-5) per ng/m(3) of B[a]P as indicator of the total PAH content, namely, lifetime exposure to 0.1 ng/m(3) would theoretically lead to one extra cancer case in 100,000 exposed individuals. This concentration of 0.1 ng/m(3) of B[a]P is suggested as a health-based guideline. Because the carcinogenic potency of fluoranthene has been estimated to be approximately 20 times less than that of B[a]P, a tentative guideline value of 2 ng/m(3) is suggested for fluoranthene. Other significant PAHs are phenanthrene, methylated phenanthrenes/anthracenes and pyrene (high air concentrations), and large-molecule PAHs such as dibenz[a,h]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, and indeno[1,2,3-cd]pyrene (high carcinogenicity). Additional source-specific indicators are benzo[ghi]perylene for gasoline vehicles, retene for wood combustion, and dibenzothiophene and benzonaphthothiophene for sulfur-containing fuels.
New Insights Into the Molecular Mechanisms of Action of Bisphosphonates (2003)

Bisphosphonates are currently the most important and effective class of anti-resorptive drugs available, but the exact molecular mechanisms by which they inhibit osteoclast-mediated bone resorption have only recently been identified. Due to the targeting of bisphosphonates to bone mineral and the ability of osteoclasts to release bone-bound bisphosphonate, a direct effect on mature osteoclasts appears to be the most important route of action. As a result of recent discoveries concerning their molecular mechanism of action, bisphosphonates can be grouped into two classes. The simple bisphosphonates that closely resemble PPi (such as clodronate, etidronate and tiludronate) can be metabolically incorporated into non-hydrolysable analogues of ATP that accumulate intracellularly in osteoclasts, resulting in induction of osteoclast apoptosis. By contrast, the more potent, nitrogen-containing bisphosphonates (such as pamidronate, alendronate, risedronate, ibandronate and zoledronate) appear to act as analogues of isoprenoid diphosphate lipids, thereby inhibiting FPP synthase, an enzyme in the mevalonate pathway. Inhibition of this enzyme in osteoclasts prevents the biosynthesis of isoprenoid lipids (FPP and GGPP) that are essential for the post-translational farnesylation and geranylgeranylation of small GTPase signalling proteins. Loss of bone-resorptive activity and osteoclast apoptosis is due primarily to loss of geranylgeranylated small GTPases. Identification of FPP synthase as the target of nitrogen-containing bisphosphonates has also helped explain the molecular basis for the adverse effects of these agents in the GI tract and on the immune system.
Phản ứng cắt mạch Polyetylenterephtalat (PET) từ vỏ chai bằng Dietylen Glycol (DEG) (2008)

Polyetylenterephtalat (PET) co nguồn gốc từ vỏ chai PET phế thải được cho phản ứng với Dietylen Glycol (DEG) tạo thanh cac oligome co phân tử lượng khac nhau phụ thuộc tỷ lệ DEG/PET. Cấu truc phân tử của sản phẩm được xac định bằng LC-MS va phân tử lượng con được xac định bằng phương phap định phân nhom chức cuối mạch. Sản phẩm cắt mạch từ DEG/PET (tỷ lệ khối lượng 1/1) la một hỗn hợp chứa cac trime, pentame, heptame co hai đầu la EG hoặc DEG. Vận tốc phản ứng phản ứng tang theo ham lượng xuc tac va phân tử lượng giảm khi tang tỷ lệ DEG/PET. Co thể dung oligome lam nguyen liệu cho phản ứng biến tinh tiếp theo để ứng dụng trong PU/polyeste.
Identification of miR-193b Targets in Breast Cancer Cells and Systems Biological Analysis of Their Functional Impact (2011)

Identification of protein targets for microRNAs (miRNAs) is a significant challenge due to the complexity of miRNA-mediated regulation. We have previously demonstrated that miR-193b targets estrogen receptor-α (ERα) and inhibits estrogen-induced growth of breast cancer cells. Here, we applied a high-throughput strategy using quantitative iTRAQ (isobaric tag for relative and absolute quantitation) reagents to identify other target proteins regulated by miR-193b in breast cancer cells. iTRAQ analysis of pre-miR-193b transfected MCF-7 cells resulted in identification of 743 unique proteins, of which 39 were down-regulated and 44 up-regulated as compared with negative control transfected cells. Computationally predicted targets of miR-193b were highly enriched (sevenfold) among the proteins whose level of expression decreased after miR-193b transfection. Only a minority of these (13%) showed similar effect at the mRNA level illustrating the importance of post-transcriptional regulation. The most significantly repressed proteins were selected for validation experiments. These data confirmed 14-3-3ζ (YWHAZ), serine hydroxyl transferase (SHMT2), and aldo-keto reductase family 1, member C2 (AKR1C2) as direct, previously uncharacterized, targets of miR-193b. Functional RNAi assays demonstrated that specific combinations of knockdowns of these target genes by siRNAs inhibited growth of MCF-7 cells, mimicking the effects of the miR-193b overexpression. Interestingly, the data imply that besides targeting ERα, the miR-193b effects include suppression of the local production of estrogens and other steroid hormones mediated by the AKR1C2 gene, thus provoking two separate molecular mechanisms inhibiting steroid-dependent growth of breast cancer cells. In conclusion, we present here a proteomic screen to identify targets of miR-193b, and a systems biological approach to mimic its effects at the level of cellular phenotypes. This led to the identification of multiple genes whose combinatorial knock-down likely mediates the strong anti-cancer effects observed for miR-193b in breast cancer cells.
Genomic instability due to V(D)J recombination-associated transposition (2006)

The first step in assembling immunoglobulin and T-cell receptors by V(D)J recombination has similarities to transposon excision. The excised transposon-like element then integrates into DNA targets at random in vitro, but whether this activity significantly threatens the genomic integrity of its host has been unclear. Here, we recover examples where the putative transposon associated with V(D)J recombination integrated into the genome of a pre-B-cell line. Transposition accounted for a surprisingly high proportion (one-third) of integrations, while most of the remaining events had parallels to other aberrant V(D)J recombination pathways linked to oncogenic translocation. In total, transposition occurred approximately once every 50,000 V(D)J recombinations. Transposition may thus contribute significantly to genomic instability.
Chemosensitivity of human small cell carcinoma of the lung detected by flow cytometric DNA analysis of drug‐induced cell cycle perturbations in vitro (1986)

A method based on detection of drug-induced cell cycle perturbation by flow cytometric DNA analysis has previously been described in Ehrlich ascites tumors as a way to estimate chemosensitivity. The method is extended to test human small-cell carcinoma of the lung. Three tumors with different sensitivities to melphalan in nude mice were used. Tumors were disaggregated by a combined mechanical and enzymatic method and thereafter have incubated with different doses of melphalan. After incubation the cells were plated in vitro on agar, and drug induced cell cycle changes were monitored by flow cytometric DNA analysis. Melphalan produced a dose-related S phase accumulation in the two sensitive tumors, whereas no changes in the cell cycle distribution were found in the resistant tumor. The size of S phase accumulation correlated to the chemosensitivity in vivo. For low concentrations of melphalan, the S phase accumulation was accompanied by G2 + M accumulation. The results indicate that the method may be extended to sensitivity testing of human solid tumors, including screening for new agents.
Experimental demonstration of polarization-insensitive air-cladding grating couplers for silicon-on-insulator waveguides (2014)

We present an air-cladding apodized focusing subwavelength grating that can effectively couple two polarizations into a single waveguide. For the transverse magnetic mode, -3.2 dB maximum coupling efficiency with ∼28 nm 1 dB bandwidth is achieved. With the same grating, -4.3 dB maximum coupling efficiency with ∼58 nm 1 dB bandwidth is achieved for the transverse electric mode. The minimum difference between two polarizations' coupling peaks is demonstrated to be ∼32 nm. At the 1525 nm wavelength range, the polarization-insensitive grating is demonstrated with -6.5 dB coupling efficiency. The polarization-insensitive coupling wavelength can be controlled experimentally.
Beyond antigens and adjuvants: formulating future vaccines (2016)

The need to optimize vaccine potency while minimizing toxicity in healthy recipients has motivated studies of the formulation of vaccines to control how, when, and where antigens and adjuvants encounter immune cells and other cells/tissues following administration. An effective subunit vaccine must traffic to lymph nodes (LNs), activate both the innate and adaptive arms of the immune system, and persist for a sufficient time to promote a mature immune response. Here, we review approaches to tailor these three aspects of vaccine function through optimized formulations. Traditional vaccine adjuvants activate innate immune cells, promote cell-mediated transport of antigen to lymphoid tissues, and promote antigen retention in LNs. Recent studies using nanoparticles and other lymphatic-targeting strategies suggest that direct targeting of antigens and adjuvant compounds to LNs can also enhance vaccine potency without sacrificing safety. The use of formulations to regulate biodistribution and promote antigen and inflammatory cue co-uptake in immune cells may be important for next-generation molecular adjuvants. Finally, strategies to program vaccine kinetics through novel formulation and delivery strategies provide another means to enhance immune responses independent of the choice of adjuvant. These technologies offer the prospect of enhanced efficacy while maintaining high safety profiles necessary for successful vaccines.
Essentials of JWST Flux Calibration (2010)

Planning for the JWST absolute flux calibration has begun. The science goal is to be able to predict the absolute flux of stars for all JWST instruments to sub-1% accuracy. A set of A0V, solar, and white dwarfs stars have been picked as a preliminary calibration sample. These stars have been observed with a combination of the Spitzer and Hubble Space Telescopes. The mapping of these stars to the sensitivities of the JWST instruments is shown along with the comparison of the Hubble and Spitzer flux calibrations. The Hubble calibration is ∼1% lower than the official IRAC calibration of Reach et al. (2005) and 1.5% lower than the proposed calibration of Rieke et al. (2008).
Epigenetics and Metabolism (2015)

The molecular signatures of epigenetic regulation and chromatin architectures are fundamental to genetically determined biological processes. Covalent and post-translational chemical modification of the chromatin template can sensitize the genome to changing environmental conditions to establish diverse functional states. Recent interest and research focus surrounds the direct connections between metabolism and chromatin dynamics, which now represents an important conceptual challenge to explain many aspects of metabolic dysfunction. Several components of the epigenetic machinery require intermediates of cellular metabolism for enzymatic function. Furthermore, changes to intracellular metabolism can alter the expression of specific histone methyltransferases and acetyltransferases conferring widespread variations in epigenetic modification patterns. Specific epigenetic influences of dietary glucose and lipid consumption, as well as undernutrition, are observed across numerous organs and pathways associated with metabolism. Studies have started to define the chromatin-dependent mechanisms underlying persistent and pathophysiological changes induced by altered metabolism. Importantly, numerous recent studies demonstrate that gene regulation underlying phenotypic determinants of adult metabolic health is influenced by maternal and early postnatal diet. These emerging concepts open new perspectives to combat the rising global epidemic of metabolic disorders.
NEW YORK ACADEMY OF MEDICINE, SECTION OF NEUROLOGY AND PSYCHIATRY, AND THE NEW YORK NEUROLOGICAL SOCIETY (1950)

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[Improvement on microwave technology of extracting polysaccharide from yacon leaves]. (2007)

According to the extraction ratio of polysaccharide in yacon leaves, the comparison between microwave extraction and traditional hot water extraction was conducted, and the two-factor and three-level experiment on the microwave extraction of polysaccharide from yacon leaves was investigated. The result showed that the extraction ratio of polysaccharide by using microwave extraction was better than that by using traditional hot water extraction. Moreover, according to the result of variance analysis and multiple comparison, the optimum conditions for extraction of polysaccharide by using microwave technology from yacon leaves were as follows: 280W microwave power for 2 times and 15 minutes at every time.
FUNDAMENTAL CONDITION OF GLASS FORMATION* (1947)

A bstract The fundamental condition for glass formation is the existence of strongly bonded large networks or long chains of atoms in the liquid. Such chains or networks prevent or hinder the orderly orientation of atoms in the chains or networks themselves and also that of atoms, not in the networks in the liquid, near the melting point or liquidus temperature. A liquid structure will persist when the liquid is cooled below the melting point or liquidus temperature and will continue until free, long‐range movement of atoms becomes impossible. In order to obtain such long chains or large networks, it is necessary that (1) the bond strength of atoms in the chains or networks be very strong; (2) the small ring formation of these strongly bonded atoms be at a minimum; and (3) the relative numbers of various atoms in the chains or networks be such that at least a continuous chain is configuratively and structurally possible; at the same time, the coordination numbers of the glassforming atoms should be as small as possible to keep the bond strong. The general treatment is applied to all kinds of glasses, particularly oxide glasses. The bond strengths of all M—O single‐bond linkages in these glasses are calculated and tabulated here for the first time. The bond strength of all the glassformers was found to be greater than 80 kilocalories per Avogadro bond, that of the intermediates between 60 and 80, and that of the modifiers below 60; the transition is continuous, and the division into glassformers, intermediates, and modifiers is arbitrary.
A role for microtubule bundles in the morphogenesis of chicken erythrocytes. (1991)

The acquisition of cellular asymmetry is one of the post-mitotic events in development. The cells of the avian erythropoietic lineage acquire a simple invariant asymmetry as they mature. Erythrocytes develop in suspension from spherical through discoid to lentil-shaped (lentiform) cells, with a single rigorously specified microtubule bundle, the marginal band. We show here that developing erythrocytes can express highly asymmetric morphologies, including elongated cell bodies and long processes in response to two stimuli: mechanical manipulation (repeated washing) and exposure to cytochalasin D. These experiments suggest that erythrocytes pass through a developmental stage during which microtubules are able to exert elongating forces on the cell. That stage is one in which these cells normally change shape, from spherical to discoid. The results suggest that microtubules may both guide and drive the formation of the marginal band and the characteristic morphology of these cells.
Antigenic sites on porin of Haemophilus influenzae type b: mapping with synthetic peptides and evaluation of structure predictions (1992)

The major surface-located protein in the outer membrane of Haemophilus influenzae type b (Hib) is porin, molecular mass, 38 kDa, 341 amino acids. To define precisely the molecular reactivities of nine mouse monoclonal antibodies (MAbs) against Hib porin, overlapping hexapeptides corresponding to the entire sequence of porin were synthesized. The epitopes recognized by the MAbs were mapped by enzyme-linked immunosorbent assay to stretches of 6 to 11 amino acids. Antigenic sites between amino acids 112 and 126, 148 and 153, 162 and 172, and 318 and 325 were identified. The antigenic sites between amino acids 162 and 172 and between amino acids 318 and 325 were determined by flow cytometry to be on the bacterial cell surface. Four MAbs, POR.2, POR.3, POR.4, and POR.5, that react with amino acids 162 to 172 were able to discriminate among porins from the three major outer membrane protein subtypes of Hib, i.e., 1H, 2L, and 6U. A model for the topological organization of Hib porin was created by calculating the hydrophobicity, amphiphilicity, and turn propensity in its amino acid sequence. Determination of the molecular reactivities of the anti-Hib porin MAbs provided substantive evidence for the orientation of selected regions of porin in the outer membrane of Hib.
Long-Term Stability of an Area-Reversible Atom-Interferometer Sagnac Gyroscope (2006)

We report the first demonstration of a matter-wave interference gyroscope that meets both the short-term noise and long-term stability requirements for high accuracy navigation. This performance level resulted from implementation of a novel technique to precisely reverse the input axis of the gyroscope.
Monolithic integration of multiple sensors on a single silicon chip (2016)

We present a monolithic integration of multiple sensors that have various structures and different packages. The multi sensors include an electrical field sensor (EFS) and accelerometers in a vacuum package and pressure, humidity, temperature sensors with direct contact with environment. To conduct the electrical signals from the vacuum package, three-dimensional (3-D) integration is implemented with a silicon interposer with through-silicon-vias (TSVs). The configuration of the multi sensor system, the co-design and co-fabrication, and packaging schemes are presented.