gebhart/patrepeval huggingface dataset:
VEGF <sub>165</sub> Expressed by a Replication-Deficient Recombinant Adenovirus Vector Induces Angiogenesis In Vivo (1995)

To evaluate the concept that localized delivery of angiogenic factors via virus-mediated gene transfer may be useful in the treatment of ischemic disorders, the replication-deficient adenovirus (Ad) vector AdCMV.VEGF165 (where CMV is cytomegalovirus and VEGF is vascular endothelial growth factor) containing the cDNA for human VEGF165, a secreted endothelial cell-specific angiogenic growth factor, was constructed. Human umbilical vein endothelial cells (HUVECs) and rat aorta smooth muscle cells (RASMCs) infected with AdCMV.VEGF165 (5 and 20 plaque-forming units [pfu] per cell) demonstrated VEGF mRNA expression and protein secretion into the supernatant. Furthermore, the conditioned medium from these cells enhanced vascular permeability in vivo. In contrast, neither VEGF mRNA nor secreted protein was found in uninfected HUVECs or RASMCs or in cells infected with the control vector AdCMV.beta gal (where beta gal is beta-galactosidase). Assessment of starved HUVECs at 14 days demonstrated sixfold more cells for AdCMV.VEGF165-infected HUVECs (20 pfu per cell) than for either infected or uninfected control cells. RASMC proliferation was unaffected by infection with AdCMV.VEGF165. When plated in 2% serum on dishes precoated with reconstituted basement membrane (Matrigel), HUVECs infected with AdCMV.VEGF165 (20 pfu per cell) differentiated into capillary-like structures. Under similar conditions, both uninfected HUVECs and HUVECs infected with AdCMV.beta gal did not differentiate. To evaluate the ability of AdCMV.VEGF165 to function in vivo, either AdCMV. VEGF165 or AdCMV.beta gal (2 x 10(10) pfu) was resuspended in 0.5 mL Matrigel and injected subcutaneously into mice. Immunohistochemical staining demonstrated VEGF in the tissues surrounding the Matrigel plugs containing AdCMV.VEGF165 up to 3 weeks after injection, whereas no VEGF was found in the control plugs with AdCMV.beta gal. Two weeks after injection, there was histological evidence of neovascularization in the tissues surrounding the Matrigel containing AdCMV.VEGF165, whereas no significant angiogenesis was observed in response to AdCMV.beta gal. Furthermore, the Matrigel plugs with AdCMV.VEGF165 demonstrated hemoglobin content fourfold higher than the plugs with AdCMV.beta gal. Together, these in vitro and in vivo studies are consistent with the concept that Ad vectors may provide a useful strategy for efficient local delivery of VEGF165 in the treatment of ischemic diseases.
Label-Free Cell Separation Using a Tunable Magnetophoretic Repulsion Force (2012)

This paper describes a new label-free cell separation method using a magnetic repulsion force resulting from the magnetic susceptibility difference between cells and a paramagnetic buffer solution in a microchannel. The difference in the magnetic forces acting on different-sized cells is enhanced by adjusting the magnetic susceptibility of the surrounding medium, which depends on the concentration of paramagnetic salts, such as biocompatible gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA), dissolved therein. As a proof-of-concept demonstration, Gd-DTPA solutions at concentrations of 0-80 mM were applied to separate U937 cells from red blood cells (RBCs) and to distinguish two different-sized polystyrene (PS) beads (8 and 10 μm in diameter). By increasing the Gd-DTPA concentration from 0 to 40 mM, the separation resolution of PS beads was increased from 0.08 to 0.91. Additionally, we successfully achieved label-free separation of U937 cells from RBCs with >90% purity and 1 × 10(5) cells/h throughput using a 40 mM Gd-DTPA solution.
Using NOAA AVHRR data to estimate maize production in the United States Corn Belt (1996)

Abstract Crop production assessments are extremely valuable because of their economic importance in influencing international trade and national economic policies. This study investigates using the Vegetation Condition Index derived from NOAA/AVHRR satellite data to estimate the maize production for the United States Corn Belt. Satellite data from 1985 to 1992 are utilized within a model and explain more than 50 per cent of the variation in the normalized yields from 42 Crop Reporting Districts. Results estimating the regional maize production are encouraging, and operational estimates using this model would be available for about two months prior to the maize harvest in the Corn Belt.
Reduced Mismatch Negativity is Associated with Increased Plasma Level of Glutamate in First-episode Psychosis (2017)

Reduced amplitude of mismatch negativity (MMN) is one of the more promising biological markers of schizophrenia. This finding holds true in both early and chronic phases of the disorder, and is compatible with the glutamatergic dysfunction hypothesis. To further establish MMN as a biomarker of aberrant glutamatergic neurotransmission, an exploration for an association with blood levels of glutamatergic amino acids is an important next step. Despite a large body of work investigating MMN in schizophrenia, no previous studies have undertaken this endeavor. Nineteen patients with first-episode psychosis (FEP), 21 ultra-high risk individuals (UHR), and 16 healthy controls (HC) participated in the study. The MMNs in response to duration change (dMMN) and frequency change (fMMN) were measured. The fasting plasma levels of glutamate, glutamine, glycine, D-serine, and L-serine were measured. dMMN amplitudes were significantly reduced in FEP and UHR, compared to HC. The plasma levels of glutamate of FEP were significantly higher than those of HC. Higher plasma levels of glutamate were associated with smaller dMMN amplitudes in the FEP and HC groups. These findings are compatible with the hypothesis that MMN is a useful biological marker of aberrant glutamatergic neurotransmission in the early stages of schizophrenia.
Shortening the Product Development Cycle (1992)

OVERVIEW:As techniques like cross-functional development teams and concurrent engineering become widespread, these approaches to shortening development cycles lose their competitive edge. Decisive advantage is likely to come from the techniques competitors are not using. The authors explain that there are other untapped sources of cycle time reduction for R&D managers to exploit. These include opportunities to accelerate the "fuzzy front end," in which half of a typical development cycle vanishes before the team even starts work. The authors caution against structuring a development process around a company's largest projects because this can excessively delay smaller ones. They also question the use of phased development systems, which often cause delays in their attempts to standardize control of projects.
The Extent of the Odontoblast Process in Human Dentin (1979)

Impacted human third molar teeth were examined, both with the light and electron microscope, to determine the extent of the odontoblast process. The odontoblast process was found to be limited to the inner third of coronal dentin and inner half of mid-root dentin.
Temporally consistent high frame-rate upsampling with motion sparsification (2016)

This paper continues our work on occlusion-aware temporal frame interpolation (TFI) that employs piecewise-smooth motion with sharp motion boundaries. In this work, we propose a triangular mesh sparsification algorithm, which allows to trade off computational complexity with reconstruction quality. Furthermore, we propose a method to create a background motion layer in regions that get disoccluded between the two reference frames, which is used to get temporally consistent interpolations among frames interpolated between the two reference frames. Experimental results on a large data set show the proposed mesh sparsification is able to reduce the processing time by 75%, with a minor drop in PSNR of 0.02 dB. The proposed TFI scheme outperforms various state-of-the-art TFI methods in terms of quality of the interpolated frames, while having the lowest processing times. Further experiments on challenging synthetic sequences highlight the temporal consistency in traditionally difficult regions of disocclusion.
Asymptotic iterative multi-user detection of random CDMA (2003)

Recent work has focused on designing efficient receivers for code-division multiple-access (CDMA) channels with forward error control (FEC) coding. In particular with the advent of turbo coding, new receiver structures employing iterative processing have been proposed. A variety of iterative receivers, where multi-user detectors with different complexities are used, have been investigated. In this paper we study such decoders from a large system perspective, where K, N /spl rarr/ /spl infin/. We introduce the variance transfer (VTR) functions which accurately describe the convergence of the complete decoder, and are used to predict performance behavior, such as the onset of the turbo cliff, and the maximal supportable user load /spl alpha/=K/N for a given FEC code and interference suppression method. Closed-form expressions for the simple interference canceller as well as the MMSE filter are used. Among the results we present the guidelines on the choice of error control codes, maximal system loads and accurate performance predictions for given system configurations.
Quantum Cascade Laser-Based Photoacoustic Spectroscopy for Trace Vapor Detection and Molecular Discrimination (2010)

We report on the development of a microelectromechanical systems (MEMS)-scale photoacoustic sensor for the detection of trace gases. A mid-infrared quantum cascade laser (QCL) was used to determine detection limits for acetic acid, acetone, 1,4-dioxane, and vinyl acetate. The source was continuously tunable from 1015 cm(-1) to 1240 cm(-1), allowing for the collection of photoacoustic vibrational spectra for these gases. Exceptional agreement between the measured photoacoustic spectra and the infrared spectra for acetic acid, acetone, 1,4-dioxane, and vinyl acetate was observed. Partial least-squares (PLS) regression was used to develop an algorithm for classification of these compounds based solely on photoacoustic spectra.
THE HYDROLYSIS OF HYALURONIC ACID BY BACTERIAL ENZYMES (1940)

An improved method is described for preparing the enzyme which hydrolyzes the polysaccharide acid contained in vitreous humor, umbilical cord, synovial fluid, and the mucoid phase of group A hemolytic streptococci. Preparations have been obtained from pneumococci, group A hemolytic streptococci, Clostridium welchii, and from splenic tissue, which display the same specific activity. Evidence is presented to show that the hydrolytic enzyme is not the same as that responsible for the lysis of pneumococci. In pneumococci and hemolytic streptococci the major portion of the enzyme is bound to the cell structure. The enzyme from Clostridium welchii is associated with other carbohydrate-splitting enzymes in the culture medium and not with the bacterial cells. It is suggested that the disappearance of the mucoid capsule of group A hemolytic streptococci is due to enzymatic hydrolysis of the acid polysaccharide. The relation between enzyme activity and the virulence and invasiveness of group A hemolytic streptococci is briefly considered.
Development and evaluation of a thermal display for material identification and discrimination (2007)

The objective of this study was to develop and evaluate a thermal display that assists in object identification in virtual environments by simulating the thermal cues associated with making contact with materials with different thermal properties. The thermal display was developed based on a semi-infinite body model. Three experiments were conducted to evaluate the performance of the display. The first experiment compared the ability of subjects' to identify various materials, which were presented physically or simulated with the thermal display. The second experiment examined the capacity of subjects to discriminate between a real and simulated material based on thermal cues. In the third experiment, the changes in skin temperature that occurred when making contact with real and simulated materials were measured to evaluate how these compare to theoretical predictions. The results indicated that there was no significant difference in material identification and discrimination when subjects were presented with real or simulated materials. The changes in skin temperature were comparable for real and simulated materials and were related to the contact coefficient of the material palpated, consistent with the semi-infinite body model. These findings suggest that a thermal display is capable of facilitating object recognition when visual cues are limited.
215. The chemistry of simple heterocyclic systems. Part II. Condensations of 4-chloro-6- and 4-chloro-7-nitroquinazoline with amines (1949)

J. S. Morley and J. C. E. Simpson, J. Chem. Soc., 1949, 1014 DOI: 10.1039/JR9490001014
A bead-based microfluidic approach to integrated single-cell gene expression analysis by quantitative RT-PCR (2014)

Gene expression analysis at the single-cell level is critical to understanding variations among cells in heterogeneous populations.
Monolithic fabrication of multiple-pitch gratings for WDM devices (1996)

We propose a monolithic fabrication method of multiple-pitch gratings by using a single computer-generated hologram (CGH). Even though minimum feature-size of CGH is larger than 2 /spl mu/m, we can obtain sub-/spl mu/m gratings with few angstroms pitch spacing.
CD40L-Tri, a novel formulation of recombinant human CD40L that effectively activates B cells (2012)

CD40L has a well-established role in enhancing the immunostimulatory capacity of normal and malignant B cells, but a formulation suitable for clinical use has not been widely available. Like other TNF family members, in vivo and in vitro activity of CD40L requires a homotrimeric configuration, and growing evidence suggests that bioactivity depends on higher-order clustering of CD40. We generated a novel formulation of human recombinant CD40L (CD40L-Tri) in which the CD40L extracellular domain and a trimerization motif are connected by a long flexible peptide linker. We demonstrate that CD40L-Tri significantly expands normal CD19+ B cells by over 20- to 30-fold over 14 days and induces B cells to become highly immunostimulatory antigen-presenting cells (APCs). Consistent with these results, CD40L-Tri-activated B cells could effectively stimulate antigen-specific T responses (against the influenza M1 peptide) from normal volunteers. In addition, CD40L-Tri could induce malignant B cells to become effective APCs, such that tumor-directed immune responses could be probed. Together, our studies demonstrate the potent immune-stimulatory effects of CD40L-Tri on B cells that enable their expansion of antigen-specific human T cells. The potent bioactivity of CD40L-Tri is related to its ability to self-multimerize, which may be facilitated by its long peptide linker.
<i>In vivo</i> MRS measurement of deoxymyoglobin in human forearms (1990)

In vivo MRS measurement of deoxymyoglobin (deoxy-Mb) in human forearms was performed by observing the N-delta proton of F8 proximal histidine. The concentration of deoxy-Mb reflects the oxygen level in the muscle. In resting muscles, the deoxy-Mb level was below the detection sensitivity. When ischemia was introduced by arterial occlusion, the time-resolved NMR spectra (with 1 min resolution) was recorded to follow the change in the deoxy-Mb signal. The result shows that deoxy-Mb signal builds up and levels off within 6 min. This technique may be used to study bioenergetics of muscle during exercise and under pathological conditions.
Current progress, in crystallographic studies of new Upases from filamentous fungi (1994)

Lipases from filamentous fungi have been studied extensively over many years. They exhibit properties attractive for industrial applications, e.g. in laundry detergents, tanning and paper industries and stereospecific organic synthesis. Enzymes from the fungi Rhizomucor miehei and Geotrichum candidum have been among the first neutral lipases to be characterized structurally by X-ray diffraction methods. In this paper we report a preliminary account of crystallographic studies of three other fungal lipases homologous to that from R. miehei and obtained from Humicola lanuginosa, Penicillium camembertii and Rhizopus delemar. These newly characterized structures have important implications for our understanding of structure-function relationships in lipases in general and the molecular basis of interfacial activation.
Gravimetric monitoring of gas-reservoir water influx — A combined flow- and gravity-modeling approach (2008)

Abstract Knowledge of the magnitude and distribution of water influx can be essential for managing water-drive gas fields. Geophysical fieldwide monitoring can give valuable information, particularly offshore where well control is sparse and observation wells are expensive. Advances in the accuracy of seafloor time-lapse gravimetry have made this method feasible. It can quantify which areas are flooded, providing information complementary to well-monitoring, production, and 4D seismic data. Gravimetric monitoring may aid material-balance calculations, which are vital for assessing reservoir-drive mechanism and estimating initial and remaining gas volumes. In addition, it can constrain reservoir simulation models. Our goal is to produce better physical insight into typical density changes occurring in water-drive gas fields and their associated surface-gravity response. It is feasible to monitor displacement of gas by water in reservoirs that are only a few meters thick. Gravimetric monitoring can detect edgewater encroachment in early stages. With current accuracy, the method is applicable for gas reservoirs of modest size (∼2×108m3 in situ gas volume) at medium depths (∼2km).
Bronchial Biopsies in Asthma: An Ultrastructural, Quantitative Study and Correlation with Hyperreactivity (1989)

Little is known of the structural changes in mild asthma. We have studied the light and electron microscopic structure of lobar bronchial biopsies taken at fiberoptic bronchoscopy from 11 atopic asthmatics, four of whom were symptomatic and seven of whom were asymptomatic. The former and three of the latter had bronchial hyperresponsiveness to methacholine (PC20 less than 4 mg/ml). Quantitative comparisons were made with biopsies from ten control subjects with normal airway reactivity; five had hay fever and five were nonatopic healthy volunteers. Complete absence of surface epithelium was found in three cases of symptomatic asthma, and stratified squamous epithelium was present in the fourth. A biopsy from one of the healthy control subjects had also lost its surface epithelium. The degree of epithelial loss in all subjects correlated with the degree of airway reactivity (rs = 0.67, p less than 0.001). The reticular lamina of the epithelial basement membrane showed a trend toward thickening in the seven hyperreactive asthmatics (p less than 0.001: median test). There was a tendency to high numbers of inflammatory cells in the lamina propria, but not in the submucosa, of asthmatics, but the differences between groups did not achieve statistical significance. There were significant alterations (px2 less than 0.001) in the proportions of each type of inflammatory cell found in the lamina propria and submucosa of symptomatic asthmatics: an increase of irregularly shaped lymphocytes contributed most to the observed alteration. Where surface epithelium was present, intraepithelial lymphocytes formed the major proportion of intraepithelial "migratory" cells: 64% in normal control subjects, 78% in subjects with hay fever, and 87% in asymptomatic asthmatics.(ABSTRACT TRUNCATED AT 250 WORDS)
A Surface Sol−Gel Process of TiO<sub>2</sub> and Other Metal Oxide Films with Molecular Precision (1997)

Fabrication of metal oxide thin films with thickness control of nanometer precision is an important technology that leads to wide practical applications. The present study demonstrates that a surface sol−gel process, composed of chemisorption of alkoxide, rinsing, hydrolysis, and drying, is a superior technique for the preparation of ultrathin metal oxide films with molecular precision. The simplicity of this process should be a great advantage in commercial production of nanosized oxide films.
Polarimetric X-band weather radar measurements in the tropics: radome and rain attenuation correction (2012)

Abstract. A polarimetric X-band radar has been deployed during one month (April 2011) for a field campaign in Fortaleza, Brazil, together with three additional laser disdrometers. The disdrometers are capable of measuring the raindrop size distributions (DSDs), hence making it possible to forward-model theoretical polarimetric X-band radar observables at the point where the instruments are located. This set-up allows to thoroughly test the accuracy of the X-band radar measurements as well as the algorithms that are used to correct the radar data for radome and rain attenuation. For the campaign in Fortaleza it was found that radome attenuation dominantly affects the measurements. With an algorithm that is based on the self-consistency of the polarimetric observables, the radome induced reflectivity offset was estimated. Offset corrected measurements were then further corrected for rain attenuation with two different schemes. The performance of the post-processing steps was analyzed by comparing the data with disdrometer-inferred polarimetric variables that were measured at a distance of 20 km from the radar. Radome attenuation reached values up to 14 dB which was found to be consistent with an empirical radome attenuation vs. rain intensity relation that was previously developed for the same radar type. In contrast to previous work, our results suggest that radome attenuation should be estimated individually for every view direction of the radar in order to obtain homogenous reflectivity fields.
Attenuated total reflection IR spectroscopy as a tool to investigate the structure, orientation and tertiary structure changes in peptides and membrane proteins (2000)

During the last few years, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) has become one of the most powerful methods to determine the structure of biological materials and in particular of components of biological membranes, like proteins that cannot be studied by x-ray crystallography and NMR. ATR-FTIR requires a little amount of material (1-100 microg) and spectra are recorded in a matter of minutes. The environment of the molecules can be modulated so that their conformation can be studied as a function of temperature, pressure, pH, as well as in the presence of specific ligands. For instance, replacement of amide hydrogen by deuterium is extremely sensitive to environmental changes and the kinetics of exchange can be used to detect tertiary conformational changes in the protein structure. Moreover, in addition to the conformational parameters that can be deduced from the shape of the infrared spectra, the orientation of various parts of the molecule can be estimated with polarized IR. This allows more precise analysis of the general architecture of the membrane molecules within the biological membranes. The present review focuses on ATR-IR as an experimental approach of special interest for the study of the structure, orientation, and tertiary structure changes in peptides and membrane proteins.