gebhart/patrepeval huggingface dataset:
Comparison of Ca2+-activated proteinase enzyme and inhibitor levels in cerebral cortex from normal and Alzheimer disease cases (1989)

Conference Article| February 01 1989 Comparison of Ca2+-activated proteinase enzyme and inhibitor levels in cerebral cortex from normal and Alzheimer disease cases BRENDA LAUFFART; BRENDA LAUFFART *M.R.C. Neurochemical Pathology Unit, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, U.K. Search for other works by this author on: This Site PubMed Google Scholar CARTHAGE SMITH; CARTHAGE SMITH †Neuropathology Department, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, U.K. Search for other works by this author on: This Site PubMed Google Scholar ELAINE PERRY; ELAINE PERRY †Neuropathology Department, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, U.K. Search for other works by this author on: This Site PubMed Google Scholar JOHN McDERMOTT; JOHN McDERMOTT *M.R.C. Neurochemical Pathology Unit, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, U.K. Search for other works by this author on: This Site PubMed Google Scholar DAVID MANTLE DAVID MANTLE ‡Regional Neurological Centre, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1989) 17 (1): 206–207. https://doi.org/10.1042/bst0170206 Article history Received: June 16 1988 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation BRENDA LAUFFART, CARTHAGE SMITH, ELAINE PERRY, JOHN McDERMOTT, DAVID MANTLE; Comparison of Ca2+-activated proteinase enzyme and inhibitor levels in cerebral cortex from normal and Alzheimer disease cases. Biochem Soc Trans 1 February 1989; 17 (1): 206–207. doi: https://doi.org/10.1042/bst0170206 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: SDAT, senile dementia of the Alzheimer type, CAP, Ca2+-dependent proteinase © 1989 Biochemical Society1989 Article PDF first page preview Close Modal You do not currently have access to this content.
Structure-activity Relationships of Hygromycin A and Its Analogs: Protein Synthesis Inhibition Activity in a Cell Free System. (1997)

Several analogs of hygromycin A were tested in an Escherichia coli cell free protein synthesis inhibition assay and in a Serpulina hyodysenteriae whole cell assay. The aminocyclitol moiety is essential for antibacterial activity in both cell free and whole cell assays. However a 4'-O-allyl ether of hygromycin A aglycone showed an equivalent MIC to hygromycin A, while having a less potent IC50 in the cell free assay. Hence 6-deoxy-5-keto-D-arabino-hexofuranose can be replaced by a hydrophobic allyl group and still retain antibacterial activity. However, this replacement reduces the intrinsic protein synthesis inhibition activity. The loss of intrinsic activity with replacement by the allyl group may be compensated for by better transport into the bacterial cell. In addition to the SAR analysis, we demonstrated that the ineffectiveness of hygromycin A against Gram-negative enteric bacteria such as Escherichia coli is mainly due to the efflux mechanism (Acr A/B pump) existing widely among the enteric bacteria rather than the impermeable barrier of the outer membrane.
A battery backup 64K CMOS RAM with double level aluminum technology (1983)

A 10mW 64K CMOS RAM with auto data retention mode will be described. The RAM was fabricated with a double-level aluminum process technology. To reduce active power, a split power control technique was employed. The address access time is 80ns.
High-sensitivity, wide-dynamic-range avalanche photodiode pixel design for large-scale imaging arrays (2010)

We present a detailed design study for a novel solidstate focal plane array of silicon avalanche photodiodes (APDs), with each detector in the array capable of operating with wide dynamic range in linear or Geiger-mode. The detector array is based on back-illuminated, crystallographically etched, (100) epitaxial silicon on R-plane sapphire substrates using 27 m pixels, although other sizes could be supported as well. Analysis of the APD pixel design, which includes the photoelectron noise, dark current noise, and electronic circuit noise, shows the capability of imaging with a signal-to-noise ratio greater than 7 at -30 C and ultralow illuminance of 10 -4 lux. The detector array design will enable large focal planes, capable of imaging with high sensitivity and high speed, over a wide range of natural illumination conditions from direct sunlight to a cloudy moonless night.
Integrated Analysis of Transcript, Protein and Metabolite Data To Study Lignin Biosynthesis in Hybrid Aspen (2008)

Tree biotechnology will soon reach a mature state where it will influence the overall supply of fiber, energy and wood products. We are now ready to make the transition from identifying candidate genes, controlling important biological processes, to discovering the detailed molecular function of these genes on a broader, more holistic, systems biology level. In this paper, a strategy is outlined for informative data generation and integrated modeling of systematic changes in transcript, protein and metabolite profiles measured from hybrid aspen samples. The aim is to study characteristics of common changes in relation to genotype-specific perturbations affecting the lignin biosynthesis and growth. We show that a considerable part of the systematic effects in the system can be tracked across all platforms and that the approach has a high potential value in functional characterization of candidate genes.
Fine specificity, structure, and proteolytic susceptibility of the human lymphocyte receptor for IgE. (1985)

Properties of the Fc receptor for IgE (FC epsilon R) on cultured human B lymphoblastoid cells (RPMI 8866) were studied. Specificity for human IgE (hIgE) was demonstrated by inhibition studies with both Fc epsilon R+ intact cell and detergent-solubilized receptor preparations. No interaction of the FC epsilon R with other hIg classes or with rodent IgE was seen. In other studies, 3,3-dithiobis(sulfosuccinimidyl) propionate was used to cross-link hIgE to 125I surface-labeled 8866 cells. After detergent solubilization, the 125I receptor components were isolated by immunoprecipitation, and receptor peptides of 83 and 46 kilodalton kD were demonstrated by SDS-PAGE in the presence of reducing agents. Cross-linking performed after detergent solubilization gave identical results. Tryptic maps of the 83 and 46 kD polypeptides were identical with respect to surface-iodinated peptides; this indicates a structural homology between these components. The 83 kD component was more difficult to elute from IgE affinity columns, potentially because of an increased number of IgE binding sites per FC epsilon R molecule. Limited proteolysis studies of the purified FC epsilon R with papain and V8 protease from Staphylococcus aureus demonstrated that a 16 kD FC epsilon R fragment was rapidly produced. This component was also seen after papain treatment of intact cells, and it retained the ability to interact with anti-FC epsilon R antisera and, at least in the absence of detergent, with hIgE affinity columns. Potential relationships between the FC epsilon R and lymphokines that modulate the IgE response (IgE-binding factors) are discussed.
Detection of the number of signals innoise with bandedcovariance matrices (1996)

A new approach is presented to the array signal processing problem of detecting the number of incident signals in unknown coloured noise environments with banded covariance structure. The principle of canonical correlation analysis is applied to the outputs of two spatially separated arrays. The number of signals is determined by testing the significance of the corresponding sample canonical correlation coefficients. The new method is shown to work well in unknown coloured noise situations and does not require any subjective threshold setting. The medium/high-SNR error rate may be approximately specified at a certain prescribed level, and may be traded off against the detection performance characteristic at low SNR. Simulation results are included to illustrate the performance of the proposed canonical correlation technique (CCT). It is found that the method performs well in a wide variety of coloured background noise environments. It is also demonstrated that the method is robust in the case when the noise covariance is not truly banded.
The ecology of an Itonidid fly associated with a rust on Baccharis pilularis consanguinea (1948)

(Uploaded by Plazi from the Biodiversity Heritage Library) No abstract provided.
A design concept for reconfigurable mobile radio networks with slow frequency-hopping signaling (1990)

A design concept to support both the initial planning and the management of land mobile radio networks with slow frequency-hopping signaling is proposed. It is applicable to the networks with advanced architecture of which the Pan European networks may be an example. The design consists of two planning processes: spatial and frequency planning. A novel heuristic is used to optimize the base station locations with respect to the total interference-to-signal ratio in a network. This ensures economic coverage of the network service area as well as efficient frequency reuse. A repeated application of the same heuristic to the currently measured and/or predicted data offers powerful means to manage a network in order to keep its resources highly available to their users, as is shown in a simulation example which includes not only sources of unintentional (cochannel, adjacent channel, and intermodulation) interference but also sources of intentional (jammer) interference.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Oxydation von Silber durch Wechselstrom von 50 Hz in wäßriger Natriumchloritlösung (1971)

Abstract Mit Wechselstrom von 50 Hz erhält man bei einer Stromdichte von 10 Aldm 2 in einer wäflrigen Lösung von 80 gll Na‐Chlorit bei 6O°C und 5 min Behandlungsdauer einen glänzenden, szhwarzen und korrosionsbeständigen Überzug auf Ag oder versilberten Werkstücken. Die Schicht ist sehr dicht und gleichmäβig kristallin auf der ganzen Werkstückoberfläche. Die Oxidüberzüge erwiesen sich als weitgehend beständig in feuchter Atmosphäre (10O% RF, auch mit Schwefeldioxid) sowie in Wasser.
Structure and ion channel activity of the human respiratory syncytial virus (hRSV) small hydrophobic protein transmembrane domain (2008)

The small hydrophobic (SH) protein from the human respiratory syncytial virus (hRSV) is a glycoprotein of approximately 64 amino acids with one putative alpha-helical transmembrane domain. Although SH protein is important for viral infectivity, its exact role during viral infection is not clear. Herein, we have studied the secondary structure, orientation, and oligomerization of the transmembrane domain of SH (SH-TM) in the presence of lipid bilayers. Only one oligomer, a pentamer, was observed in PFO-PAGE. Using polarized attenuated total reflection-Fourier transform infrared (PATR-FTIR) spectroscopy, we show that the SH-TM is alpha-helical. The rotational orientation of SH-TM was determined by site-specific infrared dichroism (SSID) at two consecutive isotopically labeled residues. This orientation is consistent with that of an evolutionary conserved pentameric model obtained from a global search protocol using 13 homologous sequences of RSV. Conductance studies of SH-TM indicate ion channel activity, which is cation selective, and inactive below the predicted pK(a) of histidine. Thus, our results provide experimental evidence that the transmembrane domain of SH protein forms pentameric alpha-helical bundles that form cation-selective ion channels in planar lipid bilayers. We provide a model for this pore, which should be useful in mutagenesis studies to elucidate its role during the virus cycle.
Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho. (1976)

Polarity suppressor mutants that are conditional lethal for growth have been isolated in E. coli K12. The mutations map between the ilv and cya loci of the E. coli chromosome. Rho factor isolated from one of these ts mutants does not show transcription termination activity at any temperature tested; however, it is found to be temperature sensitive for its poly(C)-dependent ATPase activity. Unlike the previously known polarity suppressor mutants (suA and psu), the rho mutation suppresses all types of polarity. Other interesting properties of these mutants include ultraviolet sensitivity, recombination deficiency, and decreased ability to lysogenize temperate phages lambda and P1. Our results suggest that rho has an essential function in the growth and normal physiology of cells. The rho(ts) mutant allows the growth of phage lambda defective in the N gene. This result supports the model that N gene product prevents transcription termination by antagonizing rho activity.
Induction of HIV‐specific T and B cell responses with a replicating and conditionally infectious lentiviral vaccine (2008)

The development of an HIV vaccine that induces broad and potent immunity is critically needed. Viruses, including lentiviruses, have been used as vectors for ex vivo transduction of antigens into dendritic cells (DC). We hypothesized that DC transduced with a vector that allows selective infection of DC could induce potent immunity by continually priming DC. A lentiviral vector encoding HIV gag-pol without env would form viral cores in transduced DC, but would release non-infectious particles by budding into endosomes and releasing apoptotic bodies or exosomes containing viral cores. DC function by endocytosing DC-derived apoptotic bodies, and they are specialized in their ability to move endocytic contents into the cytoplasm. We postulated that endocytosis of vector cores could lead to transduction of a second round of DC. In this report, we demonstrate accumulation of viral cores inside transduced DC and show second-round transduction of immature DC that endocytose transduced DC in vitro. The effectiveness of immunization of mice with transduced DC to induce specific lymphocyte activation was assessed. Mice developed antigen-specific T cell responses and specific antibodies after immunization. Transduction of DC with a replication-competent but conditionally infectious lentivirus could be a novel vaccine strategy for HIV.
Computational simulation of microfluidics, electrokinetics, and particle transport in biological MEMS devices (1999)

Biological MEMS devices are being developed for applications in collection, monitoring, diagnostics and drug delivery. The objective of this work is to develop a simulation tool for the design, analysis and optimization of these devices. Simulations of biological MEMS devices are challenging due to the non-Newtonian behavior of biological fluids, coupling of flow with electric fields in electrophoretic separations, transport of non-homogeneous cellular structures and mixing between Newtonian and non-Newtonian fluids. This paper presents Computational Fluid Dynamic (CFD) simulations of biological fluid flow, mixing, electro-kinetics and transport of particles in microfluidic systems. A commercial CFD code, CFD-ACE+, that solves the full Navier-Stokes equations using a finite-volume approach has been adapted to perform these simulations of biological MEMS devices. Model predictions are validated through comparisons with experimental data.
Home automation using Internet of Thing (2016)

In this era of digitization and automation, the life of human beings is getting simpler as almost everything is automatic, replacing the old manual systems. Nowadays humans have made internet an integral part of their everyday life without which they are helpless. Internet of things (IoT) provides a platform that allows devices to connect, sensed and controlled remotely across a network infrastructure. In this paper we focus on home automation using smart phone and computer. The iot devices controls and monitors the electronic electrical and the mechanical systems used in various types of buildings. The devices connected to the cloud server are controlled by a single admin which facilitate a number of users to which a number of sensor and control nodes are connected. The admin can access and control all the nodes connected to each user but a single user can control only the nodes to which the user itself is connected. This whole system using Internet of Things (iot) will allow mobile devices and computers to remotely control all the functions and features of home appliances from anywhere around the world using the internet connection. The system designed is economical and can be expanded as it allows connection and controlling of a number of different devices.
On scatter in megavoltage x‐ray transmission imaging (in English) (2000)

This thesis considers the role of scatter in electronic portal imaging devices (EPIDs). The thesis involved a direct measurement of scatter in an EPID. An already established Monte Carlo code, developed at the Royal Marsden Hospital, Sutton/UK, was extended and agreed with the experimental data for phantom‐to‐detector distances larger than 10 cm. An analytical scatter model was developed on the basis of the Monte Carlo analysis. In this model scatter was estimated by convolving the input beam profile with an analytical scatter kernel. The model was tested over a wide range of clinical parameters. For a 6 MV x‐ray beam impinging upon a water‐equivalent phantom, the absolute error in the scatter‐to‐primary ratio was better than 1% for a 100 field. The accuracy dropped to 4% for a 400 field. The model was implemented for a camera‐based EPID with a converter comprising a 1‐mm‐thick copper plate bonded to a 134 phosphor foil. Algorithms were developed for correcting for scatter contributions in megavoltage CT (MVCT) and for reconstruction of input beam profiles. For the latter an overall accuracy of better than 4% was obtained for fields of 100 in size. In MVCT the yield in contrast resolution after a scatter correction was small. However, a scatter correction reduced the discrepancy of 30% between true and reconstructed electron densities to 10%. In both applications the limit in accuracy is a direct consequence of the performance of the converter, which strongly overestimates multiply scattered photons of low energy. Within the framework of a convolution model multiply scattered radiation exiting an inhomogeneous phantom cannot be calculated with an accuracy exceeding the limits given.
An Advanced Real-Time Multiple Object Tracker in Variant Outdoor Environments (2012)

Tracking of humans in dynamic scenes has been an important topic of research. There has been considerable work in tracking humans and other objects in recent years. A real-time method for tracking multiple moving objects based on effective Gaussian Mixture Model (GMM), and identifying the moving objects with Joint Probability Data Association Filter (JPDAF) is proposed in this paper. Most tracking algorithms have better performance under static background but get worse results under background with fake motions. Therefore, most of the tracking algorithms are used in indoor environment. An adaptive Gaussian Mixtures has a nice property in resolving this problem. This paper uses recursive equations to constantly update the parameters of a Gaussian Mixture Model and to simultaneously select the appropriate number of components for each pixel. Therefore, this method is more time and memory efficient than the common GMM with the fixed component number. In tracking multiple moving objects, problems occur when objects pass across each other. The JPDAF method is used in this paper to solve this problem. Moreover, it can effectively deal with the various scenes such as the indoor scene, the outdoor scene, and the cluttered scene. The experimental results on our test sequence demonstrate the high efficiency of the proposed method.
Autoantibody Signature in Human Ductal Pancreatic Adenocarcinoma (2007)

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy characterized by rapid progression, invasiveness, and resistance to treatment. It is the fourth leading cause of cancer death with a 2% 5-year survival rate. Biomarkers for its early detection are lacking. This study was designed to use a proteomics-based approach as a means of identifying antigens that elicit a humoral response in PDAC patients. Antibodies against PDAC-associated antigens are useful for early cancer diagnosis and therapy. Proteins from PDAC cell lines were separated by 2-DE, and the serum IgG reactivity of 70 PDAC patients, 40 healthy subjects (HS), 30 non-PDAC tumor patients, and 15 chronic pancreatitis (CP) patients was tested by Western blot analysis. Spots specifically recognized by PDAC sera and revealed by mass spectrometry corresponded to metabolic enzymes or cytoskeletal proteins. Most were up-regulated in PDAC tissues. Thus, it seems that metabolic enzymes and cytoskeletal proteins are specific targets of the humoral response during PDAC. The results of further studies of these serological-defined antigens could be of diagnostic and therapeutic significance in PDAC.
Synthesis of Enantiomerically Pure Substituted Cyclopentenes from (−)‐Quinic Acid (1983)

The synthesis of a large variety of enantiomerically pure substituted reactive cyclopentenes 16, 23, 24 and 28 have been synthesized from the readily available (−)‐quinic acid 1 . The straightforward strategy involves a high‐yielding intramolecular aldolization‐dehydration of acyclic 1,6‐dialdehydes 13, 18, 19 and 27 obtained by oxidative cleavage of cyclohexanediols 5, 7, 11 and 12 , using either lead tetraacetate or triphenylbismuth carbonate. Neither sulfoxide formation nor racemization of the intermediate dialdehydes at the oxygenated chiral centre was observed. Transformation of the thioacetal 25 to the corresponding ketone 26 using phenylselenic anhydride is also described.
ChemInform Abstract: Synthesis of Constrained Thiorphan Analogues as Inhibitors of Neutral Endopeptidase. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ZBDD-Based Backtrack Search SAT Solver. (2002)

We introduce a new approach to Boolean satisfiability that combines backtrack search techniques and zero-suppressed binary decision diagrams (ZBDDs). This approach implicitly represents satisfiability instances using ZBDDs, and performs search using an efficient implementation of unit propagation on the ZBDD structure. We describe how to perform backtrack search using ZBDDs as the underlying structure for clause representation. This methodology, which adapts backtrack search algorithms to such implicit representations, allows for a potential exponential increase in the size of the problems that can be handled. Our experimental results show consistent speedups over conventional approaches.