gebhart/patrepeval huggingface dataset:
Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture (1988)

Articular chondrocytes are known to be phenotypically unstable in culture. One condition that has been reported to suppress dedifferentiation is cultivation at high density on tissue-culture plastic. The aim of the experiments described here was to study the effect of seeding density on chondrocyte proliferation and 35SO4 incorporation, and on the types of collagen and proteoglycan synthesized. I found that cells seeded at low or high density reached the same final density at confluence, and that 35SO4 incorporation, while initially higher (per cell) in high-density cultures, fell under both conditions, reaching the same low level after 3 weeks. The proportion of cells expressing keratan sulphate fell in low- but not high-density cultures and the decline was not prevented by inhibition of cell division. In all the cultures cells expressing keratan sulphate tended to have a rounded morphology. After 21 days in culture, chondrocytes grown at high density expressed predominantly large proteoglycans that aggregated with hyaluronic acid, whereas in low-density cultures a smaller, non-aggregating form was also present. By 21 days in culture cells at both high and low density were expressing type I collagen, although the high-density cells also had an extensive extracellular matrix of type II collagen. These observations support the conclusion that high seeding density stabilizes the chondrocyte phenotype to a greater extent than low seeding density. They also suggest that enhanced dedifferentiation at low density may be due to cell spreading, rather than to selective proliferation of a phenotypically unstable subpopulation of cells.
EFFICACY OF INTRAPORTAL INFUSION OF PROSTAGLANDIN E1 TO IMPROVE THE HEPATIC BLOOD FLOW AND GRAFT VIABILITY IN PORCINE LIVER TRANSPLANTATION (1997)

This study demonstrates that the intraportal infusion of PGE1 improves hepatic allograft blood flow, predominantly through an effect on HAF, and may improve graft viability after orthotopic liver transplantation.
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of <i>Escherichia coli</i> by R-Factor DNA (1972)

Transformation of E. coli cells treated with CaCl(2) to multiple antibiotic resistance by purified R-factor DNA is reported. Drug resistance is expressed in a small fraction of the recipient bacterial population almost immediately after uptake of DNA, but full genetic expression of resistance requires subsequent incubation in drugfree medium before antibiotic challenge. Transformed bacteria acquire a closed circular, transferable DNA species having the resistance, fertility, and sedimentation characteristics of the parent R factor. Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R-factor DNA in this system.
Propagation of Bessel beams generated using finite-width Durnin ring (2013)

We have studied the increase of the power contained in Bessel beams generated using the Durnin ring technique, which is compatible with microelectromechanical systems technology. Increasing the ring width to increase the output power will lead to deviation from the Bessel beam profile and its diffraction properties. In this work, the effect of the ring width on the generated beam is investigated. An analytical expression for the generated beam depth of focus (DOF) is obtained. A Fourier optics model is also developed to estimate the transverse field profile. The theoretical predictions are assisted by numerical simulations and experimental measurements. The developed models allow engineering the beam diffraction properties to make the necessary compromise between the DOF and the amount of energy carried by the beam depending on the targeted application.
Query-Chain Focused Summarization (2014)

Update summarization is a form of multidocument summarization where a document set must be summarized in the context of other documents assumed to be known. Efficient update summarization must focus on identifying new information and avoiding repetition of known information. In Query-focused summarization, the task is to produce a summary as an answer to a given query. We introduce a new task, Query-Chain Summarization, which combines aspects of the two previous tasks: starting from a given document set, increasingly specific queries are considered, and a new summary is produced at each step. This process models exploratory search: a user explores a new topic by submitting a sequence of queries, inspecting a summary of the result set and phrasing a new query at each step. We present a novel dataset comprising 22 querychains sessions of length up to 3 with 3 matching human summaries each in the consumerhealth domain. Our analysis demonstrates that summaries produced in the context of such exploratory process are different from informative summaries. We present an algorithm for Query-Chain Summarization based on a new LDA topic model variant. Evaluation indicates the algorithm improves on strong baselines.
Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan (2003)

Viral nervous necrosis (VNN) is a worldwide disease among marine fishes. In Taiwan, NNN disease was first identified in 2 species of hatchery-reared grouper, Epinephelus fuscogutatus and E. akaaya in 1994. Since then, increasing mortalities have occurred among groupers Epinephelus spp., and also among European eels Anguilla anguilla L., yellow-wax pompano Trachinotus falcatus, firespot snapper Lutaanus erythropterus B., barramundi Lates calcarifer, cobias Rachycentron canadum, humpback groupers Cromileptes altivelis and Chinese catfish Parasilurus asotus. In the present study, samples were collected from affected fishes and processed for reverse transcriptase (RT) PCR amplification and virus isolation in cell culture. Infected cells (GF-1 cell line) exhibited cytopathic-effect characteristics of grouper nervous necrosis virus (GNNV). A RT-PCR product of approximately 830 bp was amplified from the brain homogenate of tested samples and sequenced. The nucleotide and deduced amino acid sequences of the amplified RT-PCR products from all isolates were strongly homologous (> 97 %) with the corresponding region of the published sequence of red-spotted grouper nervous necrosis virus (RGNVV). Therefore, all Taiwan NNV (nervous necrosis virus) isolates studied in this report belong to the RGNNV genotype. We used 5 neutralizing monoclonal antibodies (MAbs) against GNNV to analyze the antigenic relationship of Taiwan NNV isolates and striped jack nervous necrosis virus (SJNNV). The results of neutralization tests revealed that all Taiwan NNV isolates were closely related, but antigenically different from SJNNV in 3 neutralizing epitopes. To our knowledge, this is the first description of NNV infection in European eels, yellow-wax pompano, firespot snapper, cobia and Chinese catfish, and the first reported instance of natural NNV infection in freshwater fishes causing high mortality.
Biomechanical Properties of Hip Cartilage in Experimental Animal Models (1995)

The material properties of normal adult articular cartilage were determined in the femoral head and acetabulum of baboons, dogs, and bovines, and were compared with those of normal human hip cartilage. In situ creep and recovery indentation experiments were performed using an automated creep indentation apparatus. To curvefit the entire creep curve, a numerical algorithm based on biphasic finite element methods and nonlinear optimization was developed. This effort represents the first successful use of 100% of the creep indentation curve to obtain the mechanical properties of normal articular cartilage. The results show that material properties of articular cartilage exhibit significant topographical variations in the femoral head and acetabulum, and between these two bone structures. Furthermore, significant differences exist in the mechanical properties of hip cartilage among the 4 species. Specifically, in all species the smallest aggregate modulus is found in the inferior aspect of the femoral head. Among all species, human hip cartilage is the stiffest in all test sites; bovine tissue is the softest. Human tissue has the smallest Poisson's ratio and permeability in all test sites. The aggregate modulus of human hip cartilage is closely resembled by that of baboon hip cartilage. Anatomically, canine and baboon hips exhibit similar characteristics to the human hip joint; the bovine hip joint is distinctly different. Based on this study's data, the baboon represents the most appropriate animal model of normal human hip articular cartilage.
Microneedle Biosensor for Real‐Time Electrical Detection of Nitric Oxide for In Situ Cancer Diagnosis During Endomicroscopy (2015)

A dual-diagnostic system of endom-icroscope and microneedle sensor is developed to demonstrate high-resolution imaging combined with electrical real-time detection of NO released from cancer tissues. The dual-diagnostic system can be a new platform for facile, precise, rapid, and accurate detection of cancers in various biomedical applications.
Postconditioning via stuttering reperfusion limits myocardial infarct size in rabbit hearts: role of ERK1/2 (2005)

Emerging evidence suggests that restoration of blood flow in a stuttering manner may limit lethal myocardial ischemia-reperfusion injury. However, the mechanisms contributing to this phenomenon, termed postconditioning (post-C), remain poorly defined. Our aim was to test the hypothesis that activation of classic "survival kinases," phosphatidylinositol 3-kinase (PI3-kinase) and/or extracellular signal-regulated kinase (ERK)1/2, may play a role in post-C-induced cardioprotection. In protocol 1, isolated buffer-perfused rabbit hearts underwent 30 min of sustained coronary artery occlusion and were randomized to receive abrupt reperfusion (controls) or four cycles of 30 s of reperfusion and 30 s of reocclusion before full restoration of flow (post-C). Protocol 2 was identical except control and postconditioned hearts received the PI3-kinase inhibitor LY-294002 (protocol 2A) or the ERK1/2 antagonist PD-98059 (protocol 2B) throughout the first 25 min of reperfusion, whereas in protocol 3, myocardial samples were obtained during the early minutes of reflow from additional control, postconditioned, and nonischemic sham hearts for the assessment, by standard immunoblotting, of phospho-Akt (downstream target of PI3-kinase) and phospho-ERK. Protocols 1 and 2 corroborated that infarct size (delineated by tetrazolium staining and expressed as a percent of risk region) was reduced in postconditioned hearts vs. control hearts and also revealed that post-C-induced cardioprotection was maintained despite LY-294002 treatment but was abrogated by PD-98059. These pharmacological data were supported by protocol 3, which showed increased immunoreactivity of phospho-ERK but not phospho-Akt with post-C. Thus our results implicate the involvement of ERK1/2 rather than PI3-kinase/Akt in the reduction of infarct size achieved with post-C.
Flexible compact AC transmission system - a new mode for large-capacity and long-distance power transmission (2006)

In this paper, the requirement and difficulties of large-capacity and long-distance power transmission under the background of national development strategy "transmission of electric power from the western to the eastern region" in China is analyzed. The compact transmission lines designed and constructed by China and FACTS technologies used to improve the power transmission stability and capacity in China are introduced. The preliminary study on the integrated technology predominance of compact transmission line and FACTS to enhance the transmission capability is carried out. Based on these investigations, the concept "flexible compact AC transmission system" is propounded. The inclusion and extension of this concept is analyzed: the complex and new questions of optimum-cooperating two technologies. Considering the long-distance transmission capability per unit corridor width, the flexible compact AC transmission system is a high efficient power transmission mode. It is applicable to EHV and UHV AC power transmission, and has a good developing and operating prospect.
Effects of LHRH agonists on the growth of human prostatic tumor cells: "in vitro" and "in vivo" studies. (1997)

Luteinizing Hormone Releasing Hormone (LHRH) agonists exert both "in vitro" and "in vivo" a direct inhibitory action on the growth of both androgen-dependent (LNCaP) and androgen-independent (DU 145) human prostatic cancer cell lines. The present experiments have been performed to investigate the mechanisms involved in this direct antiproliferative action of LHRH agonists. In particular, the aim was to study whether these compounds might exert their antiproliferative effect by interfering with the stimulatory action of epidermal growth factor (EGF) both "in vitro" and "in vivo". To this purpose, the effects of LHRH agonist, Zoladex (LHRH-A), on the mitogenic action of EGF, on EGF-activated intracellular signaling mechanisms (tyrosine phosphorylation of EGF receptor and c-fos proto-oncogene expression), and on the concentration of EGF receptors have been evaluated in both LNCaP and DU 145 cells. The results of these "in vitro" studies show that in LNCaP cells LHRH-A counteracts the mitogenic action of EGF, abrogates the EGF-induced c-fos expression and reduces the concentration of EGF-binding sites, without modifying the EGF induced tyrosine phosphorylation. In DU 145 cells, LHRH-A antagonizes the proliferative action of EGF, inhibits tyrosine phosphorylation of EGF receptor induced by EGF and significantly reduces the number of EGF binding sites, without altering the stimulation of c-fos expression induced by EGF. For the "in vivo" experiments, male nude mice were s.c. injected in the flank with DU 145 cells and treated for 14 days with LHRH-A (100 micrograms/days). At the end of the treatment, the concentration of EGF receptors on membrane preparations as well as on tumor volume were found to be significantly lower in LHRH-A treated animals than in control mice. The mitotic index and the expression of the proliferation-associated antigen Ki67 were found similar in control as well as in treated animals. In addition no modification of apoptotic index (expression of p53) was observed. These data suggest that LHRH agonists may inhibit the proliferation of the tumor cells by interfering with the stimulatory actions of EGF.
Estimating Human Cases of Avian Influenza A(H7N9) from Poultry Exposure (2013)

In March 2013 an outbreak of avian influenza A(H7N9) was first recognized in China. To date there have been 130 cases in human, 47% of which are in men over the age of 55.The influenza strain is a novel subtype not seen before in humans; little is known about zoonotic transmission of the virus, but it is hypothesized that contact with poultry in live bird markets may be a source of exposure. The purpose of this study is to estimate the transmissibility of the virus from poultry to humans by estimating the amount of time shoppers, farmers, and live bird market retailers spend exposed to poultry each day. Results suggest that increased risk among older men is not due to greater exposure time at live bird markets.
Separation of boron-complexed diol compounds using high-performance capillary electrophoresis (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSeparation of boron-complexed diol compounds using high-performance capillary electrophoresisJames P. Landers, Robert P. Oda, and Mark D. SchuchardCite this: Anal. Chem. 1992, 64, 22, 2846–2851Publication Date (Print):November 15, 1992Publication History Published online1 May 2002Published inissue 15 November 1992https://pubs.acs.org/doi/10.1021/ac00046a032https://doi.org/10.1021/ac00046a032research-articleACS PublicationsRequest reuse permissionsArticle Views316Altmetric-Citations62LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Templated Repair of Long Bone Defects in Rats with Bioactive Spiral-Wrapped Electrospun Amphiphilic Polymer/Hydroxyapatite Scaffolds (2015)

Effective repair of critical-size long bone defects presents a significant clinical challenge. Electrospun scaffolds can be exploited to deliver protein therapeutics and progenitor cells, but their standalone application for long bone repair has not been explored. We have previously shown that electrospun composites of amphiphilic poly(d,l-lactic acid)-co-poly(ethylene glycol)-co-poly(d,l-lactic acid) (PELA) and hydroxyapatite (HA) guide the osteogenic differentiation of bone marrow stromal cells (MSCs), making these scaffolds uniquely suited for evaluating cell-based bone regeneration approaches. Here we examine whether the in vitro bioactivity of these electrospun scaffolds can be exploited for long bone defect repair, either through the participation of exogenous MSCs or through the activation of endogenous cells by a low dose of recombinant human bone morphogenetic protein-2 (rhBMP-2). In critical-size rat femoral segmental defects, spiral-wrapped electrospun HA-PELA with preseeded MSCs resulted in laminated endochondral ossification templated by the scaffold across the longitudinal span of the defect. Using GFP labeling, we confirmed that the exogenous MSCs adhered to HA-PELA survived at least 7 days postimplantation, suggesting direct participation of these exogenous cells in templated bone formation. When loaded with 500 ng of rhBMP-2, HA-PELA spirals led to more robust but less clearly templated bone formation than MSC-bearing scaffolds. Both treatment groups resulted in new bone bridging over the majority of the defect by 12 weeks. This study is the first demonstration of a standalone bioactive electrospun scaffold for templated bone formation in critical-size long bone defects.
Comparison of Methods for Whole-Organ Decellularization in Tissue Engineering of Bioartificial Organs (2012)

Organ transplantation is now a well-established procedure for the treatment of end-stage organ failure due to various causes, but is a victim of its own success in that there is a growing disparity in numbers between the donor organ pool available for transplantation and the patients eligible for such a procedure; hence, an alternative solution to the limited donor organ pool is both desirable and necessary. Tissue engineering is an interdisciplinary field that applies the principles of engineering and life sciences toward the development of functional replacement tissues for clinical use. A recent innovation in tissue and organ engineering is the technique of whole-organ decellularization, which allows the production of complex three-dimensional extracellular matrix (ECM) bioscaffolds of the entire organ with preservation of the intrinsic vascular network. These bioscaffolds can then be recellularized to create potentially functional organ constructs as a regenerative medicine strategy for organ replacement. We review the current applications and methods in using xenogeneic whole-organ ECM scaffolds to create potentially functional bioartificial organ constructs for surgical implantation, and present a comparison of specific trends within this new and developing technique.
Targeted delivery of anti-angiogenic agent TNP-470 using water-soluble polymer in the treatment of choroidal neovascularization. (1999)

These results suggest that TNP-470-PVA inhibited the proliferation of HUVECs more sensitively than that of BRPECs, and the targeted delivery of TNP-470-PVA may have potential as a treatment modality for CNV.
Underwater image enhancement framework and its application on an autonomous underwater vehicle platform (2020)

Underwater imaging has been increasingly employed in vision-based marine research. However, the inappropriate installation of a light source and the complex underwater environment will result in the uneven illumination and overexposure on the captured images. To address these issues, an underwater image enhancement framework for autonomous underwater vehicles platform is proposed, which consists of underwater light source optimization and illumination nonuniformity correction. The light source optimization method improves the imaging quality by computing an appropriate angle of the light casting. In this way, the center of the field of view is always well lit. In addition, an adaptive filter-based illumination correction algorithm is proposed to solve the uneven illumination caused by the artificial light source. During this process, image block segmentation and the measure of image enhancement index are applied to improve the adaptability and reduce the calculation errors of the filter parameters. A dataset with real underwater images collected under different natural conditions has been built and tested. The experimental results indicate that the proposed method is more adaptive and effective than the typical methods.
Athermal germanium migration in strained silicon layers during junction formation with solid-phase epitaxial regrowth (2005)

The formation of a thin strained Si layer on top of a strain-relaxed SiGe buffer is a recent approach to improve the drive current of complementary metal-oxide-semiconductor devices by inducing strain within the transistor channel. At the same time, advanced process technologies require junction formation processes with minimal diffusion and very high dopant activation. Solid-phase epitaxial regrowth is a low temperature process based on preamorphization and subsequent regrowth leading to highly activated and shallow junctions. In this letter, we investigate the stability of the thin strained Si layer, during solid-phase epitaxial regrowth process by monitoring the Ge redistribution∕strain after the preamorphization step (without any anneal) and after the thermal regrowth process.
Finding an optimal PV panel maximum power point tracking method (2005)

The need for a maximum power point tracker in a photovoltaic system is explained. A variety of photovoltaic panel maximum power point tracking methods are then briefly compared. The optimal method from those compared, where the photovoltaic panel power-voltage curve is scanned periodically, and the maximum power point tracker is controlled by current, is discussed in detail. Problems with this implementation are highlighted, and the design of a voltage-controlled implementation using the same power-voltage curve scan is presented. Problems and advantages of this system are given.