gebhart/patrepeval huggingface dataset:
New Vector System for Random, Single-Step Integration of Multiple Copies of DNA into the <i>Rhodococcus</i> Genome (2010)

ABSTRACT We designed a new vector system for creating a random mutant library with multiple integrations of DNA fragments into the Rhodococcus genome in a single step. For this, we cotransformed two vectors into Rhodococcus by electroporation: pTip- istAB - sacB regulates the expression of the transposase (IstA) and its helper protein (IstB) under the influence of a thiostrepton-inducible promoter, and pRTSK- sacB provides the transposable-marker DNA. Both are multicopy vectors that are stable in the host cells; transposition of the transposable-marker DNA occurs only after the induction of IstA/IstB expression. With the addition of thiostrepton, all cultured cells harboring the two vectors, irrespective of the volume, can be mutated by random insertion of the transposable-marker DNA into their genome. Among the generated mutants examined, 30% showed multiple (two to five) insertion copies. The multiple integrated DNA copies were stable in the genome for more than 80 generations of serial growth without the addition of any selective antibiotics. This system can also be used for integrating various copy numbers of stably maintained protein expression cassettes in the host cell genome to modulate the expression level of biologically active recombinant proteins. We successfully applied this system to integrate multiple copies of expression cassettes for proline iminopeptidase and vitamin D 3 hydroxylase into the Rhodococcus genome and verified that the clones containing double or multiple copies of the integrated cassettes produced higher levels and showed higher enzymatic activities of the target protein than clones with only a single copy of integration.
Spray vaccination of chickens with an experimental vaccine against Newcastle disease (1977)

Chickens, of various age groups, were vaccinated against Newcastle disease by spray vaccination with an experimental vaccine, designated V5K7E1. Aerosol spraying of 3-days-old chicks having a high titre of maternal antibodies failed to induce any obvious stimulation of haemagglutination-inhibiting antibodies. The chicks, when challenged 2 and 3 weeks after vaccination, were fully protected, but protection was inadequate when they were challenged 4 to 7 weeks after vaccination. Spray vaccination of 1-day-old specific-pathogen-free chicks resulted in the presence of antibodies at 7, 14 and 21 days. Subsequently the levels of antibodies declined progressively. The chicks resisted challenge up to 42 days after vaccination. Maternally immune chicks spray - vaccinated for the first time at 14 days showed a significant increase in antibodies and remained healthy when challenged at 10 weeks of age. Booster spray vaccination at 21 days of age subsequent to primary spray vaccination at 3 days of age, induced an anamnestic rise in antibody titre that persisted at protective levels for at least 66 days. Similarly, spray vaccination at 28 days, following administration of vaccine in the drinking water at 14 days, gave rise to high antibody titres which persisted for at least 70 days. In field trials, spray vaccination at the age of 3 days or 14 days provoked little or no apparent respiratory disturbance and was followed by an average mortality of 0.9% and 1.4% respectively.
Enhanced laser cooling of rare-earth-ion-doped nanocrystalline powders (2006)

The enhanced laser cooling performance of rare-earth-ion-doped nanocrystalline powders is predicted, compared to the bulk material, using ${\mathrm{Yb}}^{3+}:{\mathrm{Y}}_{2}{\mathrm{O}}_{3}$ as the model material. This is achieved by enhancing the off-resonance, phonon-assisted absorption, which is proportional to the three factors considered in this paper: the dopant concentration, the pumping field energy, and the excitation coefficient. Using the energy transfer theory for concentration quenching, the optimum concentration corresponding to the maximum cooling power is found to be considerably larger than the currently used value, suggesting noticeable enhancement effects for laser cooling. The pumping field energy is enhanced in random nanopowders compared with bulk crystals under the same irradiation, due to the multiple scattering of photons. Photons are thus localized in the medium and do not propagate through, increasing the photon absorption of the pumping beam (and it is shown that the reabsorption of the fluorescence is negligible). Using molecular dynamics simulations, the phonon density of states (DOS) of the nanopowder is calculated, and found to have broadened modes, and extended small tails at low and high frequencies. The second-order electronic transition rate for the anti-Stokes luminescence is calculated using the Fermi golden rule, which includes the influence of this phonon DOS, and is shown to have enhancement effects on the laser cooling efficiency using nanopowders. It is finally concluded that these three enhancement mechanisms are essentially to increase the population of the three participating carriers (electron, photon, and phonon) in the interacting volume, and this also points out directions for enhancing laser cooling performance in bulk materials.
Over 95% of large-scale length uniformity in template-assisted electrodeposited nanowires by subzero-temperature electrodeposition (2011)

In this work, we report highly uniform growth of template-assisted electrodeposited copper nanowires on a large area by lowering the deposition temperature down to subzero centigrade. Even with highly disordered commercial porous anodic aluminum oxide template and conventional potentiostatic electrodeposition, length uniformity over 95% can be achieved when the deposition temperature is lowered down to -2.4°C. Decreased diffusion coefficient and ion concentration gradient due to the lowered deposition temperature effectively reduces ion diffusion rate, thereby favors uniform nanowire growth. Moreover, by varying the deposition temperature, we show that also the pore nucleation and the crystallinity can be controlled.
Mitomycin derivatives. 1. Preparation of mitosane and mitosene compounds and their biological activities (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMitomycin derivatives. 1. Preparation of mitosane and mitosene compounds and their biological activitiesShukuo Kinoshita, Keizo Uzu, Kinichi Nakano, Mikio Shimizu, Toshinaka Takahashi, and Masanao MatsuiCite this: J. Med. Chem. 1971, 14, 2, 103–109Publication Date (Print):February 1, 1971Publication History Published online1 May 2002Published inissue 1 February 1971https://pubs.acs.org/doi/10.1021/jm00284a005https://doi.org/10.1021/jm00284a005research-articleACS PublicationsRequest reuse permissionsArticle Views269Altmetric-Citations71LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Preparation Methods and Superplastic Properties of Fine-Grained Zirconia and Alumina Based Ceramics. (1999)

ジルコニアならびにアルミナ系材料を対象として,界面の微構造をコロイドプロセスにより制御したセラミックスを調整するとともに,これらの材料の超塑性特性を支配する微視的な組織因子を調べた。粒界微構造と高温力学特性との関係を変形と破壊の微視的過程の面から解析し,超塑性特性を向上させるための組織制御指針を抽出した。特に,界面損傷の微視過程の観察から,ジルコニア系の破壊がキャビティー成長型であるのに対し,アルミナ系は発生型であることを明らかにした。コロイドプロセスによって,粒界欠陥の低密度化あるいはアルミナおよび微量のSiO2による均一な粒界修飾が可能になり,ジルコニアの超塑性を向上させることに成功した。ジルコニア分散アルミナの細粒複合体では,500%を越える超塑性延性を得ることに成功した。これはアルミナ系材料で初めて実現された特性である.さらに開発した制御手法によって,粒径0.1μm以下の単斜晶ジルコニアならびにCuO添加ジルコニア緻密焼結体の常圧合成を実現し,今後の超塑性のより低温化へのプロセス制御の手法を示した。
In Vitro Activity of R126638 and Ketoconazole Against Malassezia Species (2006)

The in vitro activity of a new triazole R126638 against Malassezia yeasts was compared with that of ketoconazole. With the agar dilution technique, minimal inhibitory concentrations were lower for R126638 compared with ketoconazole against Malassezia globosa, M. obtusa, M. slooffiae, M. restricta and two strains of M. sympodialis. On human stratum corneum in vitro, both R126638 and ketoconazole were very effective in reducing the production of hyphae from 15% to 1% with R126638 and to 2% with ketoconazole. Scanning electron microscopy did not reveal obvious surface differences between untreated cultures and cultures exposed to ketoconazole or R126638 in the concentration range 0.01-1 microg/ml. However, transmission electron microscopy showed partial to complete necrosis of the cytoplasmic organelles of Malassezia yeasts. The combined scanning electron microscopy and transmission electron microscopy findings confirm earlier observations of the "mummifying" effect of azoles against Malassezia spp. In conclusion, R126638 is an interesting new triazole with high activity against the Malassezia yeasts, which are involved in pityriasis versicolor and seborrhoeic dermatitis.
Surfactant‐Assisted Synthesis of Nanoscale Gadolinium Metal–Organic Frameworks for Potential Multimodal Imaging (2008)

Less basic: By controlling the pH value of the reaction medium, two different gadolinium-containing nanoscale metal–organic frameworks (NMOFs) based on the same building blocks can be synthesized (see picture). The NMOFs carry a large payload of Gd3+ centers and are efficient contrast agents for T2-weighted magnetic resonance imaging. The NMOFs are highly luminescent when doped with other lanthanide ions such as Eu and Tb.
Accuracy of position detection using a position-sensitive detector (1998)

Position-sensitive detectors (PSD) provide four current outputs that are proportional to the two-dimensional center of mass of a light spot impinging on the face of the detector. This characteristic permits the PSD to be used for sensing the position of a light source. The application motivating the work reported here involved the tracking of a light source located on a surface with approximate dimensions 122 cm wide by 91 cm high located approximately 2 m from the detector. The diameter of the light source was a few millimeters. The work described here demonstrates the feasibility of using a PSD to track the position of a light source with accuracy better than 1 mm from this distance.
Contributory provision point contracts – a risk-free mechanism for hedging cloud energy costs (2013)

Cloud computing services rely on electricity to power compute-servers, network equipment, cooling systems, and other supporting infrastructure. As such, energy costs are a substantial outgoing to public providers of cloud computing services. On-demand pricing, where consumers are not required to give advance notice of requirements, does not aid the provider in planning future demand, and therefore makes it more difficult to purchase energy at discounted rates. In this paper, we propose an advance pricing mechanism for cloud computing resources based on provision-point contracts, commonly used by deal-of-the-day websites such as Groupon. We show how our Contributory Provision Point (CPP) contracts reward consumers with reduced prices for advance reservations, while allowing providers to make accurate forecasts of energy usage. We show how CPP contracts are risk-free for the provider, guaranteeing to be at least as profitable as on-demand mechanisms where electricity is purchased ad-hoc by the provider. Through a computer simulation, we demonstrate that CPP contracts can be more profitable for the provider compared to a traditional method of hedging electricity futures using a popular forecasting algorithm. Furthermore, we show that CPP contracts encourage consumers to forecast honestly by rewarding them with discounted rates, while remaining profitable for the provider, even when forecasts are not completely accurate.
Chapter 2 (1971)

Classical theism rests on the belief that God is infinite, that his infinity is to be understood primarily in terms of his self-existence, and that he is therefore the Creator of the world ex nihilo. But many distinguished philosophers have held that God is finite. I shall mention three — two from the ancient, and one from the modern, world.
Scalable libraries for Fortran 90D/High Performance Fortran (2002)

High Performance Fortran (HPF) is a new language, based on Fortran 90, developed by HPF Forum. The language was designed to support data parallel programming with top performance on MIMD and SIMD computers with non-uniform memory access costs. The main features of the language include the FORALL construct, new intrinsic functions and data distribution directives. A perusal of HPF shows that most of the parallelism is hidden in the runtime library. Further, efficient parallelization of FORALL construct and array assignment functions on distributed memory machines requires the use of collective communication to access non-local data. This communication could be structured (like shift, broadcast, all-to-all communication) or unstructured. Thus, the scalability of the code generated by the compiler depend on the scalability of these libraries. In this paper, we present the design and performance of an scalable library for the intrinsic functions and the collective communication library.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Surface anisotropy of polyimide film irradiated with linearly polarized ultraviolet light (1998)

Using polarized infrared (IR) absorption, we have investigated the surface anisotropy of a poly [4, 4′-oxydiphenylene-pyromellitimide] (PMDA-ODA) film that arises from anisotropic decomposition of the polyimide chain during irradiation with linearly polarized ultraviolet (LPUV) light. To monitor the surface anisotropy, we designed the sample structure so that the polyimide films decomposed uniformly over the entire film thickness. The surface anisotropy has a maximum at an irradiation energy of 105 J/cm2. For PMDA-ODA, the maximum surface anisotropy is significantly smaller than the surface anisotropy generated by rubbing. By analyzing the irradiation energy dependence of an IR absorption band, we found that the decomposition rate of the polyimide chain oriented parallel to the polarization direction of the LPUV light is greater only by ∼23% than that oriented perpendicular to it. This is the reason for the small surface anisotropy induced by the LPUV light irradiation.
Clinical significance of fetal heart rate patterns during labor. VIII. Breech presentations. (1984)

The fetal heart rate (FHR) tracings of 302 consecutive breech presentations were analyzed to assess their potential value in clinical practice. There were 274 singleton births, 27 first twins and 1 triplet. Ten percent of the gestations were greater than or equal to 42 weeks, and 26% were less than or equal to 36 weeks. Infants premature by weight (less than 2,500 gm) made up 32%. Only 33.3% had no decelerations, and 63.0% had variable or variable-late decelerations. The latter group had a significantly higher incidence of depressed neonates and neonatal deaths. When accelerations were present, there were significantly fewer depressed infants and neonatal deaths. Overall the perinatal mortality (PNM) was 7.9%; for premature infants it was 27%, postterm 3% and term 1%. There was a 31% incidence of cesarean section. The PNM, when analyzed according to route of delivery, was no different for the very-low-birth-weight, low-birth-weight and term infants. Weight-specific mortality accounted for the apparent difference among the very-low-birth-weight infants. The high incidence and pathophysiology of cord compression (for first- and second-stage labor) may explain the higher incidence of depression in breeches as compared to cephalics. FHR monitoring should be done throughout delivery in order for the physicians to intervene on time when fetal distress is imminent.
S&CNet: Monocular Depth Completion for Autonomous Systems and 3D Reconstruction (2019)

Dense depth completion is essential for autonomous systems and 3D reconstruction. In this paper, a lightweight yet efficient network (S\&CNet) is proposed to obtain a good trade-off between efficiency and accuracy for the dense depth completion. A dual-stream attention module (S\&C enhancer) is introduced to measure both spatial-wise and the channel-wise global-range relationship of extracted features so as to improve the performance. A coarse-to-fine network is designed and the proposed S\&C enhancer is plugged into the coarse estimation network between its encoder and decoder network. Experimental results demonstrate that our approach achieves competitive performance with existing works on KITTI dataset but almost four times faster. The proposed S\&C enhancer can be plugged into other existing works and boost their performance significantly with a negligible additional computational cost.
Patient diaries: can they offer psychological help to cancer patients? (2008)

Patient diaries have been successfully used in intensive care units (ICUs) to assist rehabilitation by addressing problems such as memory loss, nightmares and flashbacks that make recovery long and protracted. Many patients in our oncology critical care unit (CCU) experience similar problems to those leaving ICU, and we wanted to establish if such diaries could assist in their recovery process. Our small evaluation study revealed that patient diaries may be beneficial in supporting other patients who experience similar psychological symptoms, particularly those undergoing various cancer treatments.
Molecular design of multicomponent polymer systems. XVIII: Emulsification of high‐impact polystyrene and low density polyethylene blends into high‐impact alloys (1990)

Abstract The interfacial activity of a hydrogenated polybutadiene‐polystyrene tapered diblock copolymer, (HPB‐b‐PS) is investigated in blends of a low density polyethylene (LDPE) with a high impact polystyrene (HIPS) prepared in the melt state on a two‐roll mill. Optical and scanning electron microscopy examinations of smoothed or fracture surfaces and also surfaces obtained after THF‐extraction of PS phases demonstrate that the copolymer promotes the dispersion and interfacial adhesion of the components, whatever the composition and is able to create and stabilize particular dispersions of the rubber particles in these blends. Tensile and Charpy impact properties are also very significantly improved. All these features demonstrate that the ductility and toughness of PS and LDPE/PS blends can be closely controlled by adequate combinations of rubber particles and a HPB‐b‐PS copolymer.
Recurrences of Dermatophyte Toenail Onychomycosis during Long-Term Follow-up after Successful Treatments with Mono- and Combined Therapy of Terbinafine and Itraconazole (2013)

Itraconazole therapy was associated with higher RRs than terbinafine therapy. Combined therapy did not reduce the RRs compared to monotherapies. A difference might exist between continuous and intermittent antifungal regimens, but additional randomized clinical trials are needed for confirmation.
Regulatory T cells in acute myelogenous leukemia: is it time for immunomodulation? (2011)

The microenviroment of acute myelogenous leukemia (AML) is suppressive for immune effector cells. Regulatory T cells (Tregs) have been recognized as a contributor factor and may be recruited and exploited by leukemic cells to evade immunesurveillance. Studies have shown that the frequencies of marrow and blood Tregs are greater in patients with AML than in control patients. Although increased Tregs have been associated with a decreased risk of GVHD after allogeneic HCT and hence may impede the graft-versus-tumor effect, recent findings indicate that that this may not be the case. Because there is a need to improve outcomes of standard treatment (chemotherapy with or without allogeneic HCT) in AML, targeting Tregs present an outstanding opportunity in AML because discoveries may apply throughout its treatment. Here, we review data on the roles of Tregs in mediating immune system-AML interactions. We focused on in vitro, animal, and observational human studies of Tregs in AML biology, development, prognosis, and therapy in different settings (eg, vaccination and HCT). Manipulation of Tregs or other types of immunomodulation may become a part of AML treatment in the future.
Phase-Shifted Carrier Modulation Techniques for Cascaded H-Bridge Multilevel Converters (2015)

Phase-shifted carrier modulation is an industry standard in its application to multilevel H-bridge converters. The major advantage of this scheme over level-shifted and space vector modulation schemes is its inherent ability to evenly distribute losses between semiconductor devices. However, until now, its implementation has necessitated a capacitor voltage balancing scheme that degrades converter harmonic performance. This paper develops two phase-shifted carrier modulation schemes that avoid harmonic degradation by exploiting an extra degree of freedom in the available switching states. Simulation and experimental results are presented that confirm the correct operation of the modulation strategies.
ACID LYSIS OF RED BLOOD CELLS IN NORMAL CHILDREN (1971)

Summary Acid lysis of foetal red cells has been investigated by means of an automated procedure with the Fragiligraph. The time of red cells acid lysis is augmented in the first weeks of life and returns normal after the ninetieth day of life. The rate of acid lysis is normal only after the sixtieth day of life. The behaviour of the acid lysis is related to the disappearance of the foetal erythrocytes and to the presence of adult type of red cells.
Nonphotodynamic Roles of Methylene Blue: Display of Distinct Antimycobacterial and Anticandidal Mode of Actions (2018)

Significance of methylene blue (MB) in photodynamic therapy against microbes is well established. Previously, we have reported the antifungal potential of MB against <i>Candida albicans</i>. The present study attempts to identify additional antimicrobial effect of MB against another prevalent human pathogen, <i>Mycobacterium tuberculosis</i> (MTB). We explored that MB is efficiently inhibiting the growth of <i>Mycobacterium</i> at 15.62 <i>μ</i>g/ml albeit in bacteriostatic manner similar to its fungistatic nature. We uncovered additional cell surface phenotypes (colony morphology and cell sedimentation rate) which were impaired only in <i>Mycobacterium</i>. Mechanistic insights revealed that MB causes energy dependent membrane perturbation in both <i>C. albicans</i> and <i>Mycobacterium</i>. We also confirmed that MB leads to enhanced reactive oxygen species generation in both organisms that could be reversed upon antioxidant supplementation; however, DNA damage could only be observed in <i>Mycobacterium</i>. We provided evidence that although biofilm formation was disrupted in both organisms, cell adherence to human epithelial cells was inhibited only in <i>Mycobacterium</i>. Lastly, RT-PCR results showed good correlation with the biochemical assay. Together, apart from the well-established role of MB in photodynamic therapy, this study provides insights into the distinct antimicrobial mode of actions in two significant human pathogens, <i>Candida</i> and <i>Mycobacterium,</i> which can be extrapolated to improve our understanding of finding novel therapeutic options.