gebhart/patrepeval huggingface dataset:
Inhibition of Proteinase 3 by α1-Antitrypsin <i>In Vitro</i> Predicts Very Fast Inhibition <i>In Vivo</i> (2003)

Neutrophil proteinase 3 (Pr3) cleaves elastin and other matrix proteins, and is thought to cause lung tissue destruction in emphysema and cystic fibrosis. Its deleterious action is theoretically prevented by alpha1-antitrypsin, a serpin present in lung secretions. We have evaluated the anti-Pr3 activity of this inhibitor to decide whether it may play a physiologic proteolysis-preventing function in vivo. We show that (i). the oxidized inhibitor does not inhibit Pr3; (ii). the inhibitor competes favorably with elastin for the binding of Pr3, but is less efficient for inhibiting elastin-bound proteinase than for complexing free enzyme; and (iii). the inhibition takes place in at least two steps: the enzyme and the inhibitor first form a high-affinity reversible inhibitory complex EI* with an equilibrium dissociation constant K*i of 38 nM; EI* subsequently transforms into an irreversible complex EI with a first-order rate constant k2 of 0.04 s-1. Because the alpha1-antitrypsin concentration in the epithelial lining fluid is much higher than K*i, any Pr3 molecule released from neutrophils will be taken up as an EI* complex within much less than 1 s, indicating very efficient inhibition in vivo.
Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography (2014)

The bacterial cell wall is critical for the determination of cell shape during growth and division, and maintains the mechanical integrity of cells in the face of turgor pressures several atmospheres in magnitude. Across the diverse shapes and sizes of the bacterial kingdom, the cell wall is composed of peptidoglycan, a macromolecular network of sugar strands crosslinked by short peptides. Peptidoglycan's central importance to bacterial physiology underlies its use as an antibiotic target and has motivated genetic, structural, and cell biological studies of how it is robustly assembled during growth and division. Nonetheless, extensive investigations are still required to fully characterize the key enzymatic activities in peptidoglycan synthesis and the chemical composition of bacterial cell walls. High Performance Liquid Chromatography (HPLC) is a powerful analytical method for quantifying differences in the chemical composition of the walls of bacteria grown under a variety of environmental and genetic conditions, but its throughput is often limited. Here, we present a straightforward procedure for the isolation and preparation of bacterial cell walls for biological analyses of peptidoglycan via HPLC and Ultra Performance Liquid Chromatography (UPLC), an extension of HPLC that utilizes pumps to deliver ultra-high pressures of up to 15,000 psi, compared with 6,000 psi for HPLC. In combination with the preparation of bacterial cell walls presented here, the low-volume sample injectors, detectors with high sampling rates, smaller sample volumes, and shorter run times of UPLC will enable high resolution and throughput for novel discoveries of peptidoglycan composition and fundamental bacterial cell biology in most biological laboratories with access to an ultracentrifuge and UPLC.
Maintenance of high levels of pluripotent hematopoietic stem cells in vitro: effect of stromal cells and c-kit (1993)

We show here that mouse pluripotent hematopoietic stem cells can be maintained in vitro on stroma for at least 3 weeks at levels close to those found in bone marrow. The extent of stem cell maintenance is affected by the nature of the stromal cells. The stromal cell line S17 supported stem cells significantly better than heterogeneous, primary stromal layers or the stromal cell line Strofl-1. Stem cells cultured on S17 repopulated all hematopoietic lineages in marrow-ablated hosts for at least 10 months, indicating that this culture system maintained primitive stem cells with extensive proliferative capacity. Furthermore, we demonstrate that, while pluripotent stem cells express c-kit, this receptor appears to play only a minor role in stem cell maintenance in vitro. The addition of an antibody that blocks the interaction of c-kit with its ligand essentially abrogated myelopoiesis in cultures. However, the level of stem cells in antibody-treated cultures was similar to that found in untreated cultures. Thus, it seems likely that the maintenance of primitive stem cells in vitro depends on yet unidentified stromal cell-derived factor(s).
Ultrastructural study of the normal degeneration of the intersegmental muscles of <i>Antheraea polyphemus</i> and <i>Manduca sexta</i> (Insecta, lepidoptera) with particular reference to cellular autophagy (1977)

Abstract In studying ultrastructural changes in metamorphosis‐related degeneration of intersegmental muscles in Antheraea polyphemus , particular attention was directed to the mechanisms and timing of degradation of organelles and myofilaments. At emergence, the muscles are typical slowly contracting insect muscles, with a few dense body lysosomes and occasional autophagic vacuoles containing mitochondria. During the early phases of degradation the number of autophagic vacuoles, dense bodies, and lamellar bodies increases rapidly, along with an expansion of the Golgi system and the T system. Free glycogen particles and glycogenosomes are demonstrated by the PATAg test. Between 7 and 20 hours after ecdysis the T system continues to expand, the fibers subdivide, and the contractile system is degraded. Myofibrils fragment; myofilaments are not enclosed in isolating membranes at the time of their dissolution. The destruction of individual filaments occurs rapidly, with few intermediate stages being seen, while thick filaments tend to disappear earlier than thin filaments and Z‐line material. The process is generalized and not confined to specific regions of the fiber. Autophagy destroys cell organelles in apparent synchrony with the first signs of nuclear pycnosis. By 20 to 30 hours after emergence, the fibers are reduced to lamellae of polynucleate sarcoplasm containing no organized contractile material. The sarcoplasm is filled with autophagic vacuoles containing mitochondria, dense lamellar or residual bodies, and ribosome‐rich sarcoplasm. The number of mitochondria is drastically reduced at this time. In the final phases of involution (40–49 hours after emergence) shedding of the residual sarcoplasm precedes the expulsion of the pycnotic nuclei into the hemocoele. These results indicate that autophagy is responsible for the selective destruction of mitochondria, glycogen particles, ribosomes, and other organized sarcoplasmic structures. The one exception is the dissolution of the myofilaments, a process which remains undefined but which appears to be independent of lysosomal activity.
Tumor regression induced by intratumor administration of adenovirus vector expressing CD40 ligand and naive dendritic cells. (2000)

We have previously shown that in vivo genetic modification of tumors with an adenovirus (Ad) vector engineered to express CD40 ligand (AdmCD40L) induces tumor-specific CTLs and suppresses tumor growth in vivo. In the present study, we investigate the hypothesis that this treatment can be made more efficient with 10(2)-fold less Ad vector by also administering bone marrow-generated dendritic cells (DCs) to the tumor. Using AdmCD40L and the number of DCs that alone had no effect on tumor growth, the data show that the growth of CT26 (colon adenocarcinoma; H-2d) and B16 (melanoma; H-2b) murine s.c. tumors is significantly suppressed by direct administration of DCs into s.c. established tumors that had been pretreated with AdmCD40L 2 days previously. The antitumor effect produced by the combination therapy AdmCD40L + DCs correlated with in vivo priming of tumor-specific CTLs. The antitumor cell-mediated immunity was transferable to naive mice by spleen cells from AdmCD40L + DC-treated animals. The interactions between CD40L and CD40 within treated tumors were critical because tumor suppression was abrogated by coadministration to the tumors of neutralizing monoclonal antibody against CD40L along with the DCs. Finally, in vivo depletion and knockout mice experiments demonstrated that tumor regression produced by this combination therapy depends on CD8+ T cells, but not on CD4+ T cells. These findings should be useful in designing strategies for use of DCs and AdmCD40L in cancer immunotherapy.
Manufacture of Sodiym Sulfide: Reduction of Sodium sulfate to Sodium Sulfide at Temperatures below 800deg; C. (1936)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTManufacture of Sodiym Sulfide: Reduction of Sodium sulfate to Sodium Sulfide at Temperatures below 800deg; C.John F. M. White and Alfred H. WhiteCite this: Ind. Eng. Chem. 1936, 28, 2, 244–246Publication Date (Print):February 1, 1936Publication History Published online1 May 2002Published inissue 1 February 1936https://pubs.acs.org/doi/10.1021/ie50314a025https://doi.org/10.1021/ie50314a025research-articleACS PublicationsRequest reuse permissionsArticle Views391Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
함수비에 따른 불포화 도로성토의 동적 안정성 평가 (2014)

지진시 불포화 도로성토의 붕괴는 지하수 및 강우의 침투에 기인한 함수비의 증가가 그 원인이 됨이 지적되어왔다. 따라서, 이와 같은 지반재해의 방지를 위한 합리적 보강방안 및 적절한 설계기준의 정립을 위해 불포화도로성토의 동적안정성 및 변형모드에 대한 함수비의 영향을 연구할 필요가 있다. 본 연구에서는 불포화 도로성토의 변형 및 파괴거동에 대한 함수비의 영향을 연구하기 위해 상이한 함수비를 갖는 도로성토 모형에 대하여 동적원심모형실험을 진행하였다. 본 실험에서는 도로성토 모형에 대한 동적하중 부가시의 변위, 간극수압 및 가속도의계측을 통해 최적함수비 부근 및 최적함수비보다 높은 함수비를 갖는 불포화 도로성토에 대한 동적 거동을 고찰하였다. 이와 함께, 화상해석에 의한 변위 및 변형율 분포의 분석을 통하여 최적함수비보다 높은 함수비를 갖는 불포화 도로성토의 변형모드를 구명하였다. 이로부터 사면 천단부의 침하는 천단부 아래에서의 체적압축에 기인하며, 구속압력이 작은 사면 선단부 및 사면 표면부 부근에서는 체적팽창을 동반한 큰 전단변형이 발생함을 확인하였다.
The Pains of Endometriosis (2005)

Endometriosis is a disease defined by the presence of endometrial tissue outside of the uterus. Severe pelvic pain is often associated with endometriosis, and this pain can be diminished with therapies that suppress estrogen production. Many women with endometriosis also suffer from other chronic pain conditions. Recent studies suggest that mechanisms underlying these pains and sensitivity to estrogen involve the growth into the ectopic endometrial tissue of a nerve supply, which could have a varied and widespread influence on the activity of neurons throughout the central nervous system.
Risiko-Definition (1996)

Vollkommene (Kapital-) Märkte definieren sich unter anderem1 durch die Prämisse der Markttransparenz, die den Zustand gleicher Informationsstände der Marktteilnehmer beschreibt. Eine solche symmetrische Verteilung und gleichmäßige Verarbeitung von Informationen wird als Informationseffizienz bezeichnet.2
Reduction of Nitro Aromatic Compounds by Zero-Valent Iron Metal (1995)

The properties of iron metal that make it useful in remediation of chlorinated solvents may also lead to reduction of other groundwater contaminants such as nitro aromatic compounds (NACs). Nitrobenzene is reduced by iron under anaerobic conditions to aniline with nitrosobenzene as an intermediate product. Coupling products such as azobenzene and azoxybenzene were not detected. First-order reduction rates are similar for nitrobenzene and nitrosobenzene, but aniline appearance occurs more slowly (typical pseudo-first-order rate constants 3.5 × 10-2, 3.4 × 10-2, and 8.8 × 10-3 min-1, respectively, in the presence of 33 g/L acid-washed, 18−20 mesh Fluka iron turnings). The nitro reduction rate increased linearly with concentration of iron surface area, giving a specific reaction rate constant (3.9 ± 0.2 × 10-2 min-1 m-2 L). The minimal effects of solution pH or ring substitution on nitro reduction rates, and the linear correlation between nitrobenzene reduction rate constants and the square-root of mixing rate (rpm), suggest that the observed reaction rates were controlled by mass transfer of the NAC to the metal surface. The decrease in reduction rate for nitrobenzene with increased concentration of dissolved carbonate and with extended exposure of the metal to a particular carbonate buffer indicate that the precipitation of siderite on the metal inhibits nitro reduction.
New approach to photovoltaic arrays maximum power point tracking (2003)

The present trend for commercial telecommunication and scientific satellites is the utilization of standard platform, characterized by a high level of flexibility and reduced nonrecurring costs. One of the areas where flexibility is mandatory is the electrical primary power subsystem, due to the impact on solar array configuration and dimensions and on power conditioning unit. Use of the maximum power point tracker (MPPT) concept allows optimization of the above mentioned subsystem, maximizing the power transfer from the photovoltaic generator. The purpose of the paper is to present a new MPPT approach that allows minimization of the drawback caused by the intrinsic capacitance of the photovoltaic array (generally neglected) giving the possibility of operation with a large degree of freedom, independent from the converter topology and photovoltaic power generator, electrical network and technology.
Phase II Study of Radiotherapy and Temsirolimus versus Radiochemotherapy with Temozolomide in Patients with Newly Diagnosed Glioblastoma without <i>MGMT</i> Promoter Hypermethylation (EORTC 26082) (2016)

Temsirolimus was not superior to temozolomide in patients with an unmethylated MGMT promoter. Phosphorylation of mTORSer2448 in the pretreatment tumor tissue may define a subgroup benefitting from mTOR inhibition. Clin Cancer Res; 22(19); 4797-806. ©2016 AACR.
Creatine kinase and prostaglandin E, release from isolated Duchenne muscle (1991)

We studied the release of creatine kinase (CK) activity and prostaglandin E2 (PGE2) from isolated strips of biceps muscle from patients with Duchenne muscular dystrophy and nondystrophic control patients. CK release was significantly higher from the dystrophic samples than controls during the initial period of incubation, but this difference reduced with time of incubation. Immediate immersal of muscle strips into calcium-free bathing medium reduced the initial difference between the efflux from dystrophic and nondystrophic samples, whereas treatment with the calcium ionophore maintained the difference between the groups throughout the period of incubation (150 minutes). These results support the hypothesis that the lack of dystrophin in Duchenne muscle leads to damage to the tissue via a failure of calcium homeostasis. PGE2 release from the muscle strips followed a similar pattern to CK activity, supporting the possibility that, at least partly, the calcium-mediated damage involves activation of phospholipid hydrolysis.
Information-theoretic analysis of watermarking (2002)

An information-theoretic analysis of watermarking is presented in this paper. We formulate watermarking as a communication problem with side information at the encoder and decoder and determine the hiding capacity, which upper-bounds the rates of reliable transmission and quantifies the fundamental tradeoff between three quantities: the achievable watermarking rates and the allowed distortion levels for the information hider and the attacker. The hiding capacity is the value of a game between the information hider and the attacker. The optimal attack strategy is the solution of a particular rate-distortion problem, and the optimal hiding strategy is the solution to a channel coding problem. For several important problems, the hiding capacity is the same whether or not the decoder knows the host data set. It is also shown that existing watermarking systems in the literature operate far below capacity.
Oxyethylation and Oxypropylation of Low Molecular Alcohols (1999)

The effect of catalyst (potassium hydroxide, triethylamine, and sulfuric acid) on oxyethylation and oxypropylation of methanol, ethanol, and butanol is studied. Exemplary optimization of the process is performed at selectivity equal to 90% of the monoglycol alkyl ether fraction. It is shown that the most advantageous for oxyalkylation of methanol are the alkaline catalysts, especially triethylamine. The same relationship was observed for oxypropylation of ethanol and butanol. However, in oxyethylation of ethanol and butanol much better results are obtained using sulfuric acid as the catalyst.
Direct intra-tumoral injection of zinc-acetate halts tumor growth in a xenograft model of prostate cancer (2009)

Intracellular levels of zinc have shown a strong inverse correlation to growth and malignancy of prostate cancer. To date, studies of zinc supplementation in prostate cancer have been equivocal and have not accounted for bioavailability of zinc. Therefore, we hypothesized that direct intra-tumoral injection of zinc could impact prostate cancer growth. In this study, we evaluated the cytotoxic properties of the pH neutral salt zinc acetate on the prostate cancer cell lines PC3, DU145 and LNCaP. Zinc acetate killed prostate cancer cell lines in vitro, independent of androgen sensitivity, in a dose-dependent manner in a range between 200 and 600 microM. Cell death occurred rapidly with 50% cell death by six hours and maximal cell death by 18 hours. We next established a xenograft model of prostate cancer and tested an experimental treatment protocol of direct intra-tumoral injection of zinc acetate. We found that zinc treatments halted the growth of the prostate cancer tumors and substantially extended the survival of the animals, whilst causing no detectable cytoxicity to other tissues. Thus, our studies form a solid proof-of-concept that direct intra-tumoral injection of zinc acetate could be a safe and effective treatment strategy for prostate cancer.
Experimental evaluation of optimized ablation patterns for laser refractive surgery (2009)

A new experimental model based on plastic (Filofocon A) artificial eyes was used to study the ablation profiles and the outcomes of three state-of-the-art refractive surgery excimer lasers provided with narrow-beam flying spot and optimized algorithms (Ladarvision 4000, Alcon; Technolas 217 Z100, Bausch and Lomb; Allegretto wave Eye-Q, Wavelight). The 3-D ablation patterns produced by myopic laser corrections (-9, -6 and -3 D) on flat and spherical surfaces of Filofocon A were measured using high resolution optical profilometry. We found significant differences across lasers in the shape and depth of the ablation patterns. A comparison of the ablation patterns on flat and on spherical surfaces provided a measurement of the laser efficiency losses from the center to the periphery at each point of the spherical plastic corneas. This effect also varied across lasers, depending on their fluence (120-400 mJ/cm(2)). Estimates of the post-operative corneal shapes were obtained from the measurement on Filofocon A and plastic-corneal tissue correction factors. The predicted post-operative corneal ablation shape, ablated volume, asphericity and spherical aberration varied across lasers, as well as the relative contribution of ablation pattern designs and efficiency losses to the increased asphericity. Although the results show that the algorithms have been optimized to reduce the induction of spherical aberration, they would still benefit from the application of correction factors for efficiency effects derived from a systematic approach using experimental plastic models. These models have proved useful (1) to assess the outcomes of different lasers or ablation algorithms, (2) for precise calibration and testing of the lasers, and (3) to calculate experimental correction factors for efficiency effects.
Nonperturbative<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mi mathvariant="italic">k</mml:mi></mml:math>-body to two-body commuting conversion Hamiltonians and embedding problem instances into Ising spins (2008)

An algebraic method has been developed which allows one to engineer several energy levels including the low-energy subspace of interacting spin systems. By introducing ancillary qubits, this approach allows $\mathit{k}$-body interactions to be captured exactly using two-body Hamiltonians. Our method works when all terms in the Hamiltonian share the same basis and has no dependence on perturbation theory or the associated large spectral gap. Our methods allow problem instance solutions to be embedded into the ground energy state of Ising spin systems. Adiabatic evolution might then be used to place a computational system into its ground state.
Inhibitors targeting hepatocyte growth factor receptor and their potential therapeutic applications (2007)

The hepatocyte growth factor (HGF)/c-Met signaling pathway is important in mediating a wide range of biological activities, including embryological development, wound healing, tissue regeneration, angiogenesis, proliferation, survival, scattering, motility, invasion and morphogenic differentiation. HGF and/or c-Met are expressed at abnormally high levels in a large variety of solid tumors. Various c-Met mutations have been described in many solid tumors and some hematologic malignancies. Therefore, inhibition of the HGF/c-Met signaling pathway has great potential in molecular targeted cancer therapy. Many patent applications associated with inhibition of the HGF/c-Met axis have been published in the past few years. There are four small molecule c-Met inhibitors presently in clinical trials. This review focusses on recent patent applications for small molecule c-Met inhibitors and their potential applications in cancer therapy.
A low power keyword spotting algorithm for memory constrained embedded systems (2018)

Nowadays Voice User Interfaces (VUIs) have become popular thanks to their easiness of use that makes them accessible to the elderly and people with disability. Nevertheless, their use in embedded systems for the realization of portable devices is limited by the computation complexity, the memory requirements and power consumption of the keyword spotting (KWS) algorithms, usually based on deep neural networks. In this paper we propose a new algorithm based on convolutional neural networks for the keyword spotting task, that offers a good trade-off among accuracy, power consumption and memory footprint. To select our proposed solution, we compared different neural network architectures to select the best trade-off of these metrics. For further improvements of these performances we implemented our solution on a dedicated hardware platform as Myriad 2 by Movidius. The use of this chip has reduced inference time and energy per inference by 50%.