gebhart/patrepeval huggingface dataset:
Restoration of the intrinsic properties of human dermal papilla in vitro (2012)

The dermal papilla (DP) plays pivotal roles in hair follicle morphogenesis and cycling. However, characterization and/or propagation of human DPs have been unsatisfactory because of the lack of efficient isolation methods and the loss of innate characteristics in vitro. We hypothesized that culture conditions sustaining the intrinsic molecular signature of the human DP could facilitate expansion of functional DP cells. To test this, we first characterized the global gene expression profile of microdissected, non-cultured human DPs. We performed a 'two-step' microarray analysis to exclude the influence of unwanted contaminants in isolated DPs and successfully identified 118 human DP signature genes, including 38 genes listed in the mouse DP signature. The bioinformatics analysis of the DP gene list revealed that WNT, BMP and FGF signaling pathways were upregulated in intact DPs and addition of 6-bromoindirubin-3'-oxime, recombinant BMP2 and basic FGF to stimulate these respective signaling pathways resulted in maintained expression of in situ DP signature genes in primarily cultured human DP cells. More importantly, the exposure to these stimulants restored normally reduced DP biomarker expression in conventionally cultured DP cells. Cell growth was moderate in the newly developed culture medium. However, rapid DP cell expansion by conventional culture followed by the restoration by defined activators provided a sufficient number of DP cells that demonstrated characteristic DP activities in functional assays. The study reported here revealed previously unreported molecular mechanisms contributing to human DP properties and describes a useful technique for the investigation of human DP biology and hair follicle bioengineering.
Phosphorylation of Ribosomal Protein S6 (1981)

The time course of S6 phosphorylation and several aspects of protein synthesis have been studied in suspension cultures of HeLa cells, following transfer to fresh medium and serum. The phosphorylation of S6 is not temporally correlated with changes in polypeptide initiation and elongation rates, as judged from polysome profiles. Phosphorylation of S6 can be maximal within 30 min after transfer; elongation and initiation rates increase coordinately and more slowly, becoming maximal about 6 h after transfer, a time at which the net phosphorylation of protein S6 is greatly reduced or negligible. Recruitment of messenger RNA into polysomes is another response to fresh medium and serum; this response occurs almost as rapidly as the phosphorylation of S6. We suggest that the phosphorylation of S6 may play a role in messenger RNA recruitment.
Targeting oral cancer stemness and chemoresistance by isoliquiritigenin-mediated GRP78 regulation (2017)

Cancer stem cells (CSCs) are cells that drive tumorigenesis, contributing to metastasis and cancer recurrence as well as resistance to chemotherapy of oral squamous cell carcinomas (OSCC). Therefore, approaches to target CSCs become the subject of intense research for cancer therapy. In this study, we demonstrated that isoliquiritigenin, a chalcone-type flavonoid isolated from licorice root, exhibited more toxicity in oral cancer stem cells (OSCC-CSCs) compared to normal cells. Treatment of isoliquiritigenin not only inhibited the self-renewal ability but also reduced the expression of CSC markers, including the ALDH1 and CD44. In addition, the capacities of OSCC-CSCs to invade, metastasize and grow into a colony were suppressed by isoliquiritigenin. Most importantly, we showed that isoliquiritigenin potentiated chemotherapy along with downregulated expression of an ABC transporter that is associated with drug resistance, ABCG2. Moreover, a combination of isoliquiritigenin and Cisplatin significantly repressed the invasion and colony formation abilities of OSCC-CSCs. Our results suggested that administration of isoliquiritigenin reduced the protein expression of mRNA and membrane GRP78, a critical mediator of tumor biology. Overexpression of GRP78 reversed the inhibitory effect of isoliquiritigenin on OSCC-CSCs. Furthermore, isoliquiritigenin retarded the tumor growth in nude mice bearing OSCC xenografts. Taken together, these findings showed that isoliquiritigenin is an effective natural compound that can serve as an adjunct to chemotherapy for OSCC.
Profiles of Lewisx-containing glycoproteins and glycolipids in sera of patients with adenocarcinoma. (1990)

Oligosaccharides with Lex determinant (Gal beta 1----4[Fuc alpha 1----3]GlcNAc) are accumulated in large quantities in various adenocarcinomas. Monoclonal antibodies recognizing mono-, di-, or trimeric Lex showed a preferential staining of specific stages of human fetal tissues and various human adenocarcinomas. Thus, these carbohydrate epitopes are typical of oncodevelopmental antigens. The present study investigated the presence of Lex epitope in sera of normal individuals and cancer patients, utilizing two high-affinity monoclonal antibodies, SH1 and SH2, directed to mono- and dimeric Lex structures, respectively. The Lex antigen in serum was eluted in the void volume fraction of a gel filtration column, determined by using monoclonal antibody SH1, and found to be carried on a glycoprotein with a molecular weight of approximately 200,000. The Lex antigen was present in the void volume fraction of the majority (85%) of sera from adenocarcinoma patients. Although the Lex epitope was also detected in a smaller proportion (33%) of normal sera, its levels were significantly lower than in cancer sera. Lex antigen was also detected in serum glycolipid fraction; however, no significant differences were observed in normal and cancer sera. A double determinant solid phase immunoassay utilizing SH2 as the capture antibody and SH1 as the detecting antibody allowed direct determination of Lex levels in sera. By the use of this direct assay, the levels of serum Lex were found to increase in association with the progression of colorectal cancer (Dukes A to D). The percentage of detectability in sera from colon cancer patients was as follows: Dukes A, 20%; Dukes B, 45%; Dukes C, 67%; and Dukes D, 74%. The levels of serum Lex were also of prognostic value in Dukes C cancer patients after surgery and during postoperative follow-up.
Accuracy Assessment of Pictometry® Height Measurements Stratified by Cardinal Direction and Image Magnification Factor (2016)

The aim of this project was to ascertain if Pictometry® estimated height could be used in lieu of field-based height estimation. Height of a light pole measured with a telescopic height pole was compared to Pictometry® hyperspatial 4-inch (10.2 centimeters) multispectral imagery estimated light pole height on the campus of Stephen F. Austin State University, Nacogdoches, Texas. Average percent agreement between light pole height and Pictometry® estimated light pole height summarized by Pictometry® image magnification factors at 100%, 125%, 150%, 200%, and 300% magnification were within 98% of light pole height with percent disagreement ranging from 1.37% to 1.90%. RMSE between light pole height and Pictometry® estimated light pole height summarized by Pictometry® image magnification factors at 100%, 125%, 150%, 200%, and 300% magnification ranged from 0.14 meters to 0.17 meters. The results of Kruskal-Wallis test indicated that Pictometry® estimated light pole heights measured at the cardinal directions of north and east were more accurate than the directions of south and west, while increasing the magnification scale did not increase light pole height accuracy significantly. Pictometry® estimated height can be used in lieu of field-based height estimation for vertical features in a landscape and has the potential to revolutionize remotely sensed height measurements. This confirms the precision and accuracy of height measurements previously found for use of Pictometry® for tree, pole and building heights, slope distance, and height measurement by unmanned aerial systems.
Natural history of diabetic gastroparesis. (1999)

In this relatively large cohort of outpatients with diabetes, there was no evidence that gastroparesis was associated with a poor prognosis.
An integrated TDM architecture for AAPN networks (2005)

The Agile All-photonic Backbone Network (AAPN) architecture has been proposed by the telecommunication industry as a potential candidate for the ultra high speed Next Generation Optical Network (NGON) architecture. AAPN network structure is composed of adaptive optical core switches and edge routers in an overlaid star physical topology. The AAPN employs fast packet switching architecture for the network traffic, and the packet scheduling is the main part of the AAPN. The objective is to forward the packets to their destination with the lowest drop rate and delay, the bandwidth allocation can be either located at the core node or the edge switch. Two types of scheduling are considered in the AAPN architecture, namely the <i>centralized</i> and the <i>distributed</i> schemes. In the centralized scheme all decisions are made at the core node while in the distributed scheme, they are made at the edge nodes. In this paper, we want to compare both scheduling schemes. We would also like to propose a promising integrated TDM architecture that combines the good attributes of both centralized and distributed scheduling schemes. We shall characterize such architecture by various measures such as delay and loss probabilities.
Bias-Assisted H[sub 2] Gas Generation in HCl and KOH Solutions Using n-Type GaN Photoelectrode (2006)

We studied gas generation using a GaN photoelectrode in both HCl and KOH solutions. The photocurrent in KOH was larger than that in HCl under a small extra bias, and caused a more extensive generation at the beginning. GaN corrosion in HCl was less extended than that in KOH during photoelectrochemical reactions. The stability of the GaN photoelectrode in HCl was a result of reduction and oxidation instead of water photoelectrolysis.
ZMP trajectory generation for reduced trunk motions of biped robots (2002)

Trunk motions are typically used to stabilize the locomotion of biped robots, which can be very large in some leg trajectories. This paper proposes a method to reduce the motion range of the trunk by generating a desired trajectory of the zero-moment point (ZMP). The trajectory is determined by a fuzzy logic based upon the leg trajectories that are arbitrary selected. The resulting ZMP trajectory is similar to human's one and the ZMP continuously moves forward. The proposed scheme is simulated on a 7-degree-of-freedom biped robot. Its results indicate that the proposed ZMP trajectory increases the stability of the locomotion and thus resulting in reduction of motion range of the trunk.
The Compact Conformation of Fibronectin Is Determined by Intramolecular Ionic Interactions (1999)

Fibronectin exists in a compact or extended conformation, depending upon environmental pH and salt concentration. Using recombinant fragments expressed in bacteria and baculovirus, we determined the domains responsible for producing fibronectin's compact conformation. Our velocity and equilibrium sedimentation data show that FN2-14 (a protein containing FN-III domains 2 through 14) forms dimers in low salt. Experiments with smaller fragments indicates that the compact conformation is produced by binding of FN12-14 of one subunit to FN2-3 of the other subunit in the dimer. The binding is weakened at higher salt concentrations, implying an electrostatic interaction. Furthermore, segment FN7-14+A, which contains the alternatively spliced A domain between FN11 and 12, forms dimers, whereas FN7-14 without A does not. Segment FN12-14+A also forms dimers, but the isolated A domain does not. These data imply an association of domain A with FN12-14, and the presence of A may favor an open conformation by competing with FN2-3 for binding to FN12-14.
Acyl-CoA Synthetase 2 Overexpression Enhances Fatty Acid Internalization and Neurite Outgrowth (2004)

During neurodevelopment neurons increase phospholipid synthesis to generate additional plasma membrane that makes up the growing neurites. Compared with most cell types, neurons contain a high percentage of the polyunsaturated fatty acids (PUFAs) arachidonic acid (AA) and docosahexaenoic acid (DHA). By utilizing PC12 cell lines as a model neuronal cell line, we examined the internalization rate of AA, DHA, and non-essential oleic acid (OA), as well as their effects on neurite outgrowth. When wild type cells were differentiated, the rate of AA and DHA internalization increased 50% more than the rate of OA internalization. When media were supplemented with AA or DHA, the average neurite length was increased by approximately 40%, but supplementation with the same amount of OA had no effect. We also increased the levels of acyl-CoA synthetase-1 (ACS1) and ACS2 proteins to determine whether they contribute to PUFA internalization or neurite outgrowth. Overexpression of ACS1 increased the rate of OA internalization by 55%, and AA and DHA uptake was increased by 25%, but there was no significant change in neurite outgrowth. In ACS2-overexpressing cells, in contrast, the rate of OA internalization increased by 90%, AA by 115%, and DHA by 70%. The average aggregate neurite length in ACS2-overexpressing cells was increased by approximately 40% when the media were supplemented with PUFAs, but there was no change with OA supplementation. Taken together, these results support the hypotheses that ACSs are rate-limiting for fatty acid internalization and that ACS2 enhances neurite outgrowth by promoting PUFA internalization.
Vitrification of cesium-contaminated organic ion exchange resin (1994)

Vitrification has been declared by the Environmental Protection Agency (USEPA) as the Best Demonstrated Available Technology (BDAT) for the permanent disposal of high-level radioactive waste. Savannah River Site currently uses a sodium tetraphenylborate (NaTPB) precipitation process to remove Cs-137 from a wastewater solution created from the processing of nuclear fuel. This process has several disadvantages such as the formation of a benzene waste stream. It has been proposed to replace the precipitation process with an ion exchange process using a new resorcinol-formaldehyde resin developed by Savannah River Technical Center (SRTC). Preliminary tests, however, showed that problems such as crust formation and a reduced final glass wasteform exist when the resin is placed in the melter environment. The newly developed stirred melter could be capable of overcoming these problems. This research explored the operational feasibility of using the stirred tank melter to vitrify an organic ion exchange resin. Preliminary tests included crucible studies to determine the reducing potential of the resin and the extent of oxygen consuming reactions and oxygen transfer tests to approximate the extent of oxygen transfer into the molten glass using an impeller and a combination of the impeller and an external oxygen transfer system. These preliminary studies were used as a basis for the final test which was using the stirred tank melter to vitrify nonradioactive cesium loaded organic ion exchange resin. Results from this test included a cesium mass balance, a characterization of the semi-volatile organic compounds present in the off gas as products of incomplete combustion (PIC), a qualitative analysis of other volatile metals, and observations relating to the effect the resin had on the final redox state of the glass.
PREDICTION OF DIAGNOSIS IN ACUTE AND SUBACUTE OLIGOARTHRITIS OF UNKNOWN ORIGIN (1996)

A total of 146 consecutive patients between 18 and 60 yr of age with oligoarthritis of unknown origin (< or = 6 active joints, < or = 8 weeks duration) were examined by a variety of clinical, laboratory and microbiological investigations, and followed longitudinally for 24 weeks. Reactive arthritis was diagnosed in 46 patients (19 induced by Chlamydia trachomatis, 27 by enterobacteria), 62 had undifferentiated arthritis, eight other inflammatory arthritic diseases, 15 acute sarcoid arthritis and 15 non-inflammatory joint diseases. Group differences were found for many baseline variables, but with considerable overlap between the groups. A set of four clinical and laboratory variables (elevated CRP, genitourinary symptoms, metatarsophalangeal joint involvement. HLA B27) could predict reactive arthritis with a sensitivity of 69.2% and a specificity of 93.5%. A wide range of clinical and laboratory examinations are required to determine the final diagnosis in oligoarthritis, but individual and sets of clinical and laboratory measures may give helpful clues for the correct diagnosis.
ST2: the biomarker at the heart of GVHD severity (2015)

In this issue of Blood, Ponce et al report that high suppression of tumorigenicity 2 (ST2) levels are significantly associated with the incidence of acute graft-versus-host disease (aGVHD) and transplant-related mortality (TRM) in recipients of double-unit cord blood transplants (CBTs).(1)
A solid state direct on line starter for medium voltage induction motors with minimized current and torque pulsations (1999)

This paper describes a thyristorized static switch which is designed and implemented specifically for frequent direct-on-line starting of medium voltage large induction motors. The circuit configuration chosen utilizes back-to-back connected series thyristor strings on two of the supply lines. Both the starting current and the torque pulsations of the induction motor are minimized by a controlled, nonsimultaneous switching of motor phases to the supply. This reduces the electrical stresses on the power system and the mechanical stresses on the shaft as compared to conventional switching elements. The optimum switching strategy is found by the use of a hybrid mathematical model in ABC/dqO form. The validity of the results obtained for medium voltage, squirrel-cage induction machine are verified by the use of a physical simulator consisting of a low voltage, small induction machine and a shaft torque measuring system. The system has been in operation successfully in an iron and steel plant since 1991.
Stacking-sequence optimization for buckling of laminated plates by integer programming (1992)

Integer-programming formulations for the design of symmetric and balanced laminated plates under biaxial compression are presented. Both maximization of buckling load for a given total thickness and the minimization of total thickness subject to a buckling constraint are formulated. The design variables that define the stacking sequence of the laminate are zero-one integers. It is shown that the formulation results in a linear optimization problem that can be solved on readily available software. This is in contrast to the continuous case, where the design variables are the thicknesses of layers with specified ply orientations, and the optimization problem is nonlinear. Constraints on the stacking sequence such as a limit on the number of contiguous plies of the same orientation and limits on in-plane stiffnesses are easily accommodated. Examples are presented for graphite-epoxy plates under uniaxial and biaxial compression using a commercial software package based on the branch-and-bound algorithm.
Optimal long term imaging of a charged microparticle at the center of a Paul trap in an atmosphere near standard temperature and pressure: Experiment and stochastic model (1993)

The thermal rms fluctuation in position of a levitated microparticle in a Paul trap in N2 near standard temperature and pressure is calculated numerically from a stochastic equation of motion and is measured experimentally by long term imaging. The calculations reveal loci of minima within the lowest stability region and enhanced fluctuations as the first instability is approached. Pseudopotential results deviate below our calculations progressively with increased drive potential. This disparity grows by more than an order of magnitude near instabilities, although the pseudopotential approach provides a good asymptotic approximation for small drive potentials. Long term imaging experiments are performed in a trap modified to eliminate stray static fields at its ac ‘‘null’’ point. These experiments are well-described by the stochastic model and suggest the use of such a trap as a stage for long term fluorescence and Raman microphotography experiments on individual microparticles in a gaseous environment.
Diaspirin Cross-Linked Hemoglobin (DCLHB TM): Involvement of Adrenergic Mechanisms in the Pressor Effect (1994)

Diaspirin cross-linked Hemoglobin (DCLHb) (400 mg/kg, i.v.), a resuscitative solution, produces a pressor effect in rats and several other species. Studies were conducted to determine the role of the central nervous system and adrenal medulla in the pressor effect of DCLHb in rats. Intravenous administration of DCLHb produced an increase in blood pressure in cervical sectioned animals, which was comparable to that observed in normal rats. This indicates that the pressor effect of DCLHb was mediated through the peripheral vascular system rather than through the central nervous system. DCLHb produced a pressor effect in bilateral adrenal demedullated rats that was similar to normal rats, suggesting that the pressor effect is not through the release of catecholamines or other pressor substance from the adrenal medulla. The effects of DCLHb pretreatment on norepinephrine (0.5 microgram/kg), phenylephrine (5 micrograms/kg) and clonidine induced blood pressure and heart rate responses were also studied. DCLHb significantly potentiated the pressor response to norepinephrine and phenylephrine. Clonidine normally produces a fall in blood pressure by acting on the central alpha-adrenoceptors, and a rise in blood pressure by stimulating the peripheral vascular alpha-adrenoceptors. DCLHb produced a marked potentiation of the pressor response to clonidine (75 micrograms/kg, i.v.), that masked the central depressor effect. The specificity of the potentiation was confirmed by using phenoxybenzamine, prazosin, and yohimbine. In order to exclude the contribution of a centrally induced cardiovascular effect of clonidine, further studies were carried out in cervical sectioned rats. DCLHb markedly potentiated the pressor effect of clonidine (25 micrograms/kg, i.v.) in cervical sectioned rats. This potentiation could be attenuated by prazosin and yohimbine.(ABSTRACT TRUNCATED AT 250 WORDS)
Compressed sensing based quantization with prediction encoding for video transmission in WSN (2015)

Wireless multimedia sensor networks have limited computational resources such as bandwidth, storage and energy. To overcome these limitations, a promising technique called compressed sensing (CS) is adopted to develop the video encoding algorithm. CS is the process of acquiring and reconstructing a signal which is sparse thus reducing the computational complexity. The source video frames are converted into sparse components by applying sparsifying transform. The measurements obtained from the sparse components using CS are quantized and encoded by proposed algorithm for efficient storage and transmission. To represent the data with reduced number of bits an efficient compressed sensing based quantized prediction Huffman encoder is presented in this paper. The orthogonal matching pursuit recovery algorithm is used at the reconstruction side to get back the original sparse components. The performance of the video encoder is evaluated using compression ratio in terms of percentage. The PSNR and SSIM of the recovered frames show promising results thus prove to be compatible for wireless multimedia sensor networks.