gebhart/patrepeval huggingface dataset:
The Polyphosphate Bodies of Chlamydomonas reinhardtii Possess a Proton-pumping Pyrophosphatase and Are Similar to Acidocalcisomes (2001)

Acidocalcisomes are acidic calcium storage compartments described initially in trypanosomatid and apicomplexan parasites. In this work, we describe organelles with properties similar to acidocalcisomes in the green alga Chlamydomonas reinhardtii. Nigericin and NH(4)Cl released (45)Ca(2+) from preloaded permeabilized cells, suggesting the incorporation of a significant amount of this cation into an acidic compartment. X-ray microanalysis of the electron-dense vacuoles or polyphosphate bodies of C. reinhardtii showed large amounts of phosphorus, magnesium, calcium, and zinc. Immunofluorescence microscopy, using antisera raised against a peptide sequence of the vacuolar type proton pyrophosphatase (H(+)-PPase) of Arabidopsis thaliana which is conserved in the C. reinhardtii enzyme, indicated localization in the plasma membrane, in intracellular vacuoles, and the contractile vacuole where it colocalized with the vacuolar proton ATPase (V-H(+)-ATPase). Purification of the electron-dense vacuoles using iodixanol density gradients indicated a preferential localization of the H(+)-PPase and the V-H(+)-ATPase activities in addition to high concentrations of PP(i) and short and long chain polyphosphate, but lack of markers for mitochondria and chloroplasts. In isolated electron-dense vacuoles, PP(i)-driven proton translocation was stimulated by potassium ions and inhibited by the PP(i) analog aminomethylenediphosphonate. Potassium fluoride, imidodiphosphate, N,N'-dicyclohexylcarbodiimide, and N-ethylmaleimide also inhibited PP(i) hydrolysis in the isolated organelles in a dose-dependent manner. These results indicate that the electron-dense vacuoles of C. reinhardtii are very similar to acidocalcisomes with regard to their chemical composition and the presence of proton pumps. Polyphosphate was also localized to the contractile vacuole by 4',6-diamidino-2-phenylindole staining, suggesting, with the immunochemical data, a link between these organelles and the acidocalcisomes.
Accelerating Emulation and Providing Full Chip Observability and Controllability (2009)

The authors deploy an emulation framework that automatically transforms certain Hardware Description Language (HDL) parts of the testbench into synthesizable code to offload the software simulator and minimize the communication overhead. They also extend this architecture by adding multiple fast scan-chain paths in the design to provide full circuit observability and controllability on the fly.
Mucosal changes in gastric ulceration and their response to carbenoxolone sodium. (1975)

The epithelial differences between the normal stomach (six subjects) and 47 patients with gastric ulcers were compared. The concentrations of intraepithelial lymphocytes and polymorphonuclear leucocytes in lesser curve and prepyloric gastirc ulcers were compared, the effect of treatment with carbenoxolone sodium was studied. There is a statistically significant reduction in the total number of intraepithelial polymorphonuclear leucocytes before and after successful treatment with carbenoxolone sodium. There is also a statistically significant decrease in the quantity of intraepithelial lymphocytes in those patients with lesser curve gastric ulcers successfully treated with carbenoxolone sodium, whereas there is a significant increase in those patients with prepyloric gastric ulcers successfully treated and those patients in whom treatment failed. The value of counts of migrating white blood cells as a method of objectively assessing the effect of healing drugs upon the gastric mucosa is discussed. Pseudomonas aeruginosa was found in many specimens obtained, and evidence is presented that this was not a contaminant. Carbenoxolone appeared to increase the amount of mucus but had little effect upon the number of bacteria found. The possible contribution of Pseudomonas aeruginosa to gastric ulceration is discussed.
Endonuclease III is an iron-sulfur protein (1989)

Elemental analyses, Mössbauer, and EPR data are reported to show that endonuclease III of Escherichia coli is an iron-sulfur protein. Mössbauer spectra of protein freshly prepared from E. coli grown on 57Fe-enriched medium demonstrate that the native enzyme contains a single 4Fe-4S cluster in the 2+ oxidation state, with a net spin of zero. Upon treatment with ferricyanide, a fraction (less than 25%) of the clusters is oxidized into a state which yields an EPR spectrum near g = 2.01 typical of a 3Fe-4S cluster. The magnetic field dependence of the linear electric field effect verifies this assignment. Electron spin echo modulation on the g = 2.01 form of the protein in deuterated solvent indicates the presence of exchangeable protons in the vicinity of the 3Fe-4S cluster. The data obtained show that the [4Fe-4S]2+ cluster of the native enzyme is resistant to either oxidation or reduction, although photoreduction elicited a g = 1.94 type EPR signal characteristic of a [4Fe-4S]1+ cluster. These studies show that endonuclease III is unique in being both a DNA repair enzyme and an iron-sulfur protein. The function of the 4Fe-4S cluster remains to be established.
Efficient common-mode feedback circuits for pseudo-differential switched-capacitor stages (2002)

Novel common-mode feedback circuits are proposed for use in pseudo-differential switched-capacitor circuits. They can be implemented by incorporating just four additional capacitors (and switches) for an integrator, and only two additional capacitors for a residue gain amplifier. The circuits are applicable to very low-voltage switched-capacitor stages realized in submicron low-voltage CMOS processes.
Wide Range Carrier Frequency Offset Estimation Method using Training Symbols with Asymmetric Constellations for Burst-Mode Coherent Reception (2018)

We propose a simple feed-forward carrier frequency offset estimation method robust against timing errors in the detection of training symbols. A wide compensation range of ±0.5 times the signal baud-rate is numerically and experimentally confirmed.
Novel polycarboxylated EDTA-type cyclodextrins as ligands for lanthanide binding: study of their luminescence, relaxivity properties of Gd(iii) complexes, and PM3 theoretical calculations (2010)

Novel -type cyclodextrin (CD) derivatives, , and , bearing 6, 7 and 8 bis(carboxymethyl)amino (iminodiacetic acid) groups, respectively, were prepared, and their complexation with Eu(iii), Tb(iii) and Gd(iii) ions was studied. Luminescence titrations and mass spectrometry showed formation of multimetal complexes ( 2 to 3, mainly 3 and exactly 4 metal ions), whereas luminescence lifetime measurements revealed the presence of exchangeable water molecules. Semiempirical quantum mechanical calculations, performed by the PM3 method and assessed by DFT calculations on model ligands, indicated efficient multi-metal complexation, in agreement with the experiment. The structures showed coordination of the metal ions in the outer primary side of the CDs via 4 carboxylate O atoms, 2 N atoms and a glucopyranose O atom per metal ion. Coordination of water molecules was also predicted, in accordance with experimental results. Calculated bond lengths and angles were in agreement with literature experimental values of lanthanide complexes. Calculated energies showed that complex stability decreases in the order > > . (1)H NMR molecular relaxivity measurements for the Gd(iii) complexes of , or in water afforded values 4 to 10 times higher than the relaxivity of a commercial contrast agent at 12 MHz, and 6 to 20 times higher at 100 MHz. Solutions of and Gd(iii) complexes in human blood plasma displayed relaxivity values at 100 MHz 7 and 12 times, respectively, higher than the commercial agent. MTT tests of the Gd(iii) complexes using human skin fibroblasts did not show toxicity. Attempts to supramolecularly sensitize the luminescence of the lanthanide complexes using various aromatic CD guests were ineffective, evidently due to large guest-metal distances and inefficient inclusion. The described lanthanide complexes, could be useful as contrast agents in MRI.
A Dithering Based Technique for Improving Gray Level Reproduction Capability in Dark Areas on Plasma Display Panels (2002)

Because of the reduced number of displayable gray levels resulting from inverse gamma correction, images on a plasma display panel (PDP) exhibit undesirable false contours in dark areas. An error diffusion method has been applied to remedy this problem. However, it is computationally expensive and requires large memory resources. This paper proposes a computationally efficient dithering based technique to improve the gray level reproduction capability in dark areas. In the proposed method, multiple dithering masks ate utilized in turn to improve the gray level reproduction in dark areas. Also, in order to reduce undesirable regular patterns generated by the dithering method, positions of threshold values within a given dithering mask are changed. Compared to the error diffusion method, the proposed method requires much less computations and memory resources with a comparable gray level reproduction capability.
Effect of local change in O2 saturation of hemoglobin on cerebral vasodilation from hypoxia and hypotension (1993)

We tested the effect of certain newly synthesized allosteric modifiers of hemoglobin on the dilation induced by arterial hypoxia, arterial hypotension, and arterial hypercapnia in cerebral arterioles of anesthetized cats equipped with cranial windows for the observation of the cerebral microcirculation. The allosteric modifiers of hemoglobin are isomers of 2-(aryloxy)-2-methylpropionic acid. They shift the oxygen dissociation of hemoglobin to the right, thereby facilitating the local release of oxygen. When these compounds were applied topically by superfusion at a rate of 1 ml/min in a concentration of 0.1 mM, they had no significant effect on baseline arteriolar diameter but reduced significantly the vasodilation from arterial hypoxia and arterial hypotension. They did not influence the vasodilation from arterial hypercapnia. Spectrophotometric measurements of optical densities from pial veins 50-80 microns in diameter indicated that the superfusion with the allosteric compounds reduced hemoglobin oxygen saturation both during room air breathing and during hypoxia. We conclude that the vasodilations from arterial hypoxia and arterial hypotension are mediated by local oxygen-dependent mechanisms. The allosteric modifiers of hemoglobin may be useful as tools in investigating oxygen-dependent mechanisms.
An investigation of i-AlGaAs/n-GaAs doped-channel MIS-like FET's (DMT's)&#8212;Properties and performance potentialities (1987)

Doped-channel MIS-like FET's (DMT's) based upon an i-AlGaAs/n-GaAs structure have been investigated in detail for the purpose of clarifying their properties and performance potentialities. The DMT is unique in having two operation modes, a depletion-layer modulation mode and an electron accumulation mode, both of which are experimentally demonstrated through capacitance-voltage characteristics. Analytical and experimental results shows that the maximum drain current I <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">DSmax</inf> is more than 2.5 times that for a conventional n-AlGaAs/GaAs 2DEGFET. g <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">mmax</inf> and I <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Dsmax</inf> values obtained for 0.5- µm gate DMT's are very high, 310 mS/mm (410 mS/mm) and 650 mA/mm (800 mA,/mm) at 300 K (77 K), respectively, f <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">max</inf> is 48 GHz. f <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">T</inf> is as large as 45 GHz, which is the best data ever reported in 0.5-µm gate FET's. Moreover, the estimated electron saturation velocity is outstandingly large, 1.5 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">7</sup> cm/s (2 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">7</sup> cm/s) at 300 K (77 K), even for a thin GaAs channel layer with a 3 × 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">18</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> doping level, while Hall electron mobility is not reasonably so high, being typically 1850 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /V . s (1650 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /V . S). Preliminary power performances are also studied at 28.5 GHz. An 18-dBm (225-mW/mm) saturation output power, 6.4-dB linear gain, and 15-percent power added efficiency are achieved. A further performance improvement may be easily accomplished by gate length reduction, structure optimization, and so on. Consequently, it has been proved that DMT's have great feasibility for high-speed and high-frequency high-power device applications.
Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested <i>Corynebacterium glutamicum</i> R (2007)

In cellulosic ethanol production, pretreatment of a biomass to facilitate enzymatic hydrolysis inevitably yields fermentation inhibitors such as organic acids, furans, and phenols. With representative inhibitors included in the medium at various concentrations, individually or in various combinations, ethanol production by Corynebacterium glutamicum R under growth-arrested conditions was investigated. In the presence of various inhibitors, the 62 to 100% ethanol productivity retained by the C. glutamicum R-dependent method far exceeded that retained by previously reported methods.
Study on fluorescent lamp illumination and flicker (2004)

It is necessary completely to study and appraise characteristics of fluorescent lamp illumination. Flicker index is an important appraisal item. The fundamental frequency of flicker is twice as high as power frequency. The flicker index by electromagnetic ballast is more than 10%, and it is increasing sharply as reducing power voltage or illumination. By electronic ballast, especially one with active power factor correction, the lamp can run nearly without flicker under wide range of AC power voltage variation.
Phase 1 safety of ICOS agonist antibody JTX-2011 alone and with nivolumab (nivo) in advanced solid tumors; predicted vs observed pharmacokinetics (PK) in ICONIC. (2017)

3033 Background: JTX-2011 is an agonist monoclonal antibody that targets ICOS, Inducible CO-Stimulator of T cells. A dual mechanism of action is intended to activate antigen-specific CD4 T effector cells and selectively deplete intratumoral T regulatory cells. JTX-2011 is equally potent across human, rodent, and non-human primate species. Methods: A quantitative systems pharmacology (QSP) model describing target binding by JTX-2011 and target mediated drug disposition in blood, tumor and non-tumor tissues was based on preclinical potency and non-linear PK data across species. The model was translated to predict PK and target engagement (TE) in humans to facilitate dose selection. The QSP model predicts > 95% TE for 21 days at the top planned dose. We present safety and actual/predicted PK from a Phase 1 study of JTX-2011 alone (Part A) and safety in combination with nivo (Part B). Results: 25 subjects have been dosed, 19 in 4 cohorts of JTX-2011 alone at .003, .01, .03, and .1 mg/kg IV q 21 days, and 6 in 2 cohorts of JTX-2011 .01 mg/kg and .03 mg/kg IV plus nivo 240 mg IV q21 days. Safety data from ≥ 1 cycle is available for 12 subjects in Part A (7 ≥ 3 cycles), and 3 in Part B (all ≥ 3 cycles). PK data is available for cycle 1 of Part A. Mean age (±SD) is 60 (±10.6). Mean prior systemic therapies is > 5 (range 1-11). Tumor types include endometrial, triple negative breast, melanoma, lung, pancreatic and colorectal cancers. No dose limiting toxicities have been reported. 3 Grade 3 adverse events (AEs) were reported in 2 Part A subjects: anemia and hypoxia (unrelated SAE) at .003 mg/kg and JTX-2011 related diarrhea at .1 mg/kg. Grade 1-2 AEs in ≥2 subjects are chills, pyrexia, neck pain, dizziness, and nausea. 5 subjects had JTX-2011 related Grade 1-2 infusion reactions up to 6 hours post infusion. Non-linear exposure increase was observed. While PK at lower doses is consistent with model predictions, AUC and t 1/2 at 0.03 and 0.1 mg/kg doses are higher than predicted, suggesting higher than predicted TE. Conclusions: JTX-2011 has been well tolerated up to 0.1 mg/kg and with nivo at .01 mg/kg IV q 21 days. Greater than linear exposure increase was observed and TE may be higher than QSP model prediction. Clinical trial information: NCT02904226.
A proposed through‐flow inverse method for the design of mixed‐flow pumps (1993)

Abstract A through‐flow (hub‐to‐shroud) truly inverse method is proposed and described in this paper. It uses as a initial design specification, an imposition of mean swirl, i.e. radius times mean tangential velocity, given throughout the meridional section of the turbomachine. In the present implementation, it is assumed that the fluid is invsicid, incompressible and irrotational at inlet and the blades are supposed to have zero thickness. Only blade rows that impart to the fluid a constant work along the span will be considered. An application of this procedure to design the rotor of a mixed‐flow pump will be described in detail. The strategy used to find a suitable mean swirl distribution and the other design inputs is also described. The final blade shape and pressure distributions on the blade surface are presented, showing that it is possible to obtain feasible designs using this technique. Another advantage of this technique is the fact that it does not require large amounts of CPU time.
Basis for the Checkpoint Signal Specificity That Regulates Chk1 and Cds1 Protein Kinases (1999)

Six checkpoint Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are needed to regulate checkpoint protein kinases Chk1 and Cds1 in fission yeast. Chk1 is required to prevent mitosis when DNA is damaged by ionizing radiation (IR), whereas either kinase is sufficient to prevent mitosis when DNA replication is inhibited by hydroxyurea (HU). Checkpoint Rad proteins are required for IR-induced phosphorylation of Chk1 and HU-induced activation of Cds1. IR activates Cds1 only during the DNA synthesis (S) phase, whereas HU induces Chk1 phosphorylation only in cds1 mutants. Here, we investigate the basis of the checkpoint signal specificity of Chk1 phosphorylation and Cds1 activation. We show that IR fails to induce Chk1 phosphorylation in HU-arrested cells. Release from the HU arrest following IR causes substantial Chk1 phosphorylation. These and other data indicate that Cds1 prevents Chk1 phosphorylation in HU-arrested cells, which suggests that Cds1 actively suppresses a repair process that leads to Chk1 phosphorylation. Cds1 becomes more highly concentrated in the nucleus only during the S phase of the cell cycle. This finding correlates with S-phase specificity of IR-induced activation of Cds1. However, constitutive nuclear localization of Cds1 does not enhance IR-induced activation of Cds1. This result suggests that Cds1 activation requires DNA structures or protein activities that are present only during S phase. These findings help to explain how Chk1 and Cds1 respond to different checkpoint signals.
<title>Neurofuzzy-model-based feedback controller for shape memory alloy actuators</title> (2000)

This paper describes development of a motion controller for Shape Memory Alloy (SMA) actuators using a dynamic model generated by a neuro-fuzzy inference system. This kind of smart alloy is known to have a unique characteristic in that its shape can be controlled by temperature that can be varied by passing a current through it. Using SMA actuators, it would be possible to design miniature mechanisms for a variety of applications. Today SMA is used for valves, latches, and locks, which are automatically activated by heat. However it has not been used as a motion control device due to difficulty in the treatment of its highly non-linear strain-stress hysteresis characteristic which is further influenced by its temperature. In this project, a dynamic model of a SMA actuator is developed using ANFIS, a neuro-fuzzy inference system provided in MATLAB environment. Using neuro-fuzzy logic, the system identification of the dynamic system is performed by observing the change of state variables (displacement and velocity) responding to a known input (voltage across the SMA actuator). Then, using the dynamic model, the estimated input voltage required to follow a desired trajectory is calculated in an open-loop manner. The actual input voltage supplied to the SMA actuator is the sum of this open-loop input voltage and an input voltage calculated from an ordinary PD control scheme. This neuro- fuzzy logic-based control scheme is a very generalized scheme that can be used for a variety of SMA actuators. Experimental results are provided to demonstrate the potential for this type of controller to control the motion of the SMA actuator.
Enhanced stereo (1961)

Practices utilized in producing a stereo recording are discussed relative to the performance of stereo-sound reinforcement systems. The reproduction of these recordings results in an enhanced stereo effect.
The ASPECTA toolkit (2016)

Full Coverage Displays (FCDs) cover the interior surface of an entire room with pixels. FCDs make possible many new kinds of immersive display experiences - but current technology for building FCDs is expensive and complex, and software support for developing full-coverage applications is limited. To address these problems, we introduce ASPECTA, a hardware configuration and software toolkit that provide a low-cost and easy-to-use solution for creating full coverage systems. We outline ASPECTA’s (minimal) hardware requirements and describe the toolkit’s architecture, development API, server implementation, and configuration tool; we also provide a full example of how the toolkit can be used. We performed two evaluations of the toolkit: a case study of a research system built with ASPECTA, and a laboratory study that tested the effectiveness of the API. Our evaluations, as well as multiple examples of ASPECTA in use, show how ASPECTA can simplify configuration and development while still dramatically reducing the cost for creators of applications that take advantage of full-coverage displays.
Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves (2014)

Abstract We report the first electrochemical system for the detection of single‐nucleotide polymorphisms (SNPs) that can accurately discriminate homozygous and heterozygous genotypes using microfluidics technology. To achieve this, our system performs real‐time melting‐curve analysis of surface‐immobilized hybridization probes. As an example, we used our sensor to analyze two SNPs in the apolipoprotein E ( ApoE ) gene, where homozygous and heterozygous mutations greatly affect the risk of late‐onset Alzheimer’s disease. Using probes specific for each SNP, we simultaneously acquired melting curves for probe–target duplexes at two different loci and thereby accurately distinguish all six possible ApoE allele combinations. Since the design of our device and probes can be readily adapted for targeting other loci, we believe that our method offers a modular platform for the diagnosis of SNP‐based diseases and personalized medicine.