gebhart/patrepeval huggingface dataset:
Effects of Antibiotics on Multiplication of L Cells in Suspension Culture (1959)

In a study of effects of antibiotics on multiplication of L cells in suspension culture, positive inhibition of multiplication was found when less than 1 μg/ml of any one of the following were added to the media: actinomycins B, BV, or DIV; cycloheximide; gliotoxin; hygromycin; mitomycin C; patulin; thiostrepton; and xanthomycin. Actinomycin BV was the most active of the group tested as 1.5 mμg/ml was sufficient to cause a 50% reduction in growth of culture. While the shape of dose-response curves varied with different inhibitors, it was possible to use the linear portion as the basis of a method for quantitative estimation of concentration of inhibitor present. This method may be useful in determining the presence of cytotoxic metabolites formed in microbial fermentations.
The Gulf Stream—Barrier or Blender? (1985)

The Gulf Stream ’60 hydrographic survey has been used to examine the distribution of water properties across the Gulf Stream as a function of potential density. This survey covered a half million square miles of Slope, Gulf Stream and Sargasso Sea Waters in the western North Atlantic. Quantities plotted as a function of density are acceleration potential, potential temperature, desso1ved oxygen and potential vorticity. The transition from Sargasso Sea Water to Slope Water in the upper thermocline (σ0<27.1) is sharp and coincides closely with the dynamical boundary of the Gulf Stream, defined by the gradient of acceleration potential. This indicates that water mass exchanges across the Gulf Stream-Slope Water front are limited at these levels. Below the 27.1 Σ0 surface, the gradient of acceleration potential still reveals the position of the Stream, but there is no coincident water man boundary. This and the uniformity of potential vorticity across the Stream suggest that the deep property fields are being efficiently homogenized by mesoscale exchanges across the Gulf Stream. A cross-frontal eddy diffusivity of KH=2.5×106 cm2 s−1 estimated from oxygen flux calculations agrees well with previously published values for frontal regimes.
Notizen: Über die Umsetzung von Aluminiumwasserstoff mit Ammoniak und Aminen (1955)

Aluminiumwasserstoff setzt sich in ätherischer Lösung mit äquimolekularen Mengen Ammoniak bei - 80° quantitativ unter Bildung eines „Alazans“ AlHs NH
Morphological skeleton transforms for determining position and orientation of pre-marked objects (2003)

The feasibility of a pre-marking scheme for three-dimensional object recognition is demonstrated. The proposed scheme is based on the assumption that an object can be modeled by a small number of its distinct two-dimensional perspective projections. Circular markers are used to identify these views by determining their surface normals passing through their centers. The surface normal of the marker can be determined by analyzing the geometrical features of its acquired pseudo-ellipse image using morphological skeleton transforms. The position of the marker, on the other hand, has to be determined by acquiring two images from different viewing angles. The experimental results illustrate that the specific pseudo-Euclidean skeleton transform used can accurately determine the features of the ellipse to allow the successful application of the proposed pre-marking scheme.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Electrostatic sample-tip interactions in the scanning tunneling microscope (1993)

Local surface photovoltage (SPV) measurements were used to measure how the electric field of a scanning tunneling microscope tip perturbs the electronic band structure at Si(001), Si(111)-(7\ifmmode\times\else\texttimes\fi{}7), and H-terminated Si(111) surfaces. The results demonstrate that tip-induced band bending is important under typical STM conditions even on surfaces whose surface Fermi levels are nominally ``pinned.'' Spatially resolved measurements of band bending as a function of sample bias show that atomic-scale contrast in SPV images can result from local variations in the ability of the surface states under the tip to screen external electric fields.
Effect of pulsed and reversing electric fields on the orientation of linear and supercoiled DNA molecules in agarose gels (1988)

When linear or supercoiled DNA molecules are imbedded in agarose gels and subjected to electric fields, they become oriented in the gel matrix and give rise to an electric birefringence signal. The sign of the birefringence is negative, indicating that the DNA molecules are oriented parallel to the electric field lines. If the DNA molecules are larger than about 1.5 kilobase pairs, a delay is observed before the birefringence signal appears. This time lag, which is roughly independent of DNA molecular weight, decreases with increasing electric field strength. The field-free decay of the birefringence is much slower for the DNA molecules imbedded in agarose gels than observed in free solution, indicating that orientation in the gel is accompanied by stretching. Both linear and supercoiled molecules become stretched, although the apparent change in conformation is much less pronounced for supercoiled molecules. When the electric field is rapidly reversed in polarity, very little change in the birefringence signal is observed for linear or supercoiled DNAs if the equilibrium orientation (i.e., birefringence) had been reached before field reversal. Apparently, completely stretched, oriented DNA molecules are able to reverse their direction of migration with little or no loss of orientation. If the steady-state birefringence had not been reached before the field reversal, complicated orientation patterns are observed after field reversal. Very large, partially stretched DNA molecules exhibit a rapid decrease in orientation at field reversal. The rate of decrease of the birefringence signal in the reversing field is faster than the field-free decay of the birefringence and is approximately equal to the rate of orientation in the field (after the lag period).(ABSTRACT TRUNCATED AT 250 WORDS)
Branch trace compression for snapshot-based simulation (2006)

We present a scheme to compress branch trace information for use in snapshot-based microarchitecture simulation. The compressed trace can be used to warm any arbitrary branch predictor's state before detailed microarchitecture simulation of the snapshot. We show that compressed branch traces require less space than snapshots of concrete predictor state. Our branch-predictor based compression (BPC) technique uses a software branch predictor to provide an accurate model of the input branch trace, requiring only mispredictions to be stored in the compressed trace file. The decompressor constructs a matching software branch predictor to help reconstruct the original branch trace from the record of mispredictions. Evaluations using traces from the Journal of ILP branch predictor competition show we achieve compression rates of 0.013-0.72 bits/branch (depending on workload), which is up to 210/spl times/ better than gzip; up to 52/spl times/ better than the best general-purpose compression techniques; and up to 4.4/spl times/ better than recently-published, more general trace compression techniques. Moreover, BPC-compressed traces can be decompressed in less time than corresponding traces compressed with other methods.
Uptake of Al3+ into the N-lobe of human serum transferrin (1992)

We have studied the binding of Al3+ to human serum apotransferrin (80 kDa) and recombinant N-lobe human apotransferrin (40 kDa) in 0.1 M-sodium bicarbonate solution at a pH meter reading in 2H2O (pH*) of 8.8 using 1H n.m.r. spectroscopy. The results show that for the intact protein, preferential binding of Al3+ to the N-lobe occurs. Molecular modelling combined with an analysis of ring-current-induced shifts suggest that n.m.r. spectroscopy can be used to probe hinge bending processes which accompany metal uptake in solution.
Real-time implementation of model predictive control (2005)

A real-time implementation of model predictive control (MPC) is presented in this paper. MPC, also known as receding horizon control and moving horizon control, is widely accepted as the controller of choice for multivariable systems that have inequality constraints on system states, inputs and outputs. For processes with slow dynamics and low sampling rates, MPC is typically implemented on a dedicated computer. For systems with fast dynamics such as those in MEMS, a hardware embedded MPC would be an appropriate controller implementation since the size and the application precludes the use of a dedicated computer. Recent manufacturing advances have opened the path for the fabrication of micromechanical devices and electronic subsystems under the same manufacturing and packaging process, thereby opening the path for the use of advanced control algorithms towards systems-on-chip applications.
Use of a DC SQUID receiver preamplifier in a low field MRI system (1995)

We have used tuned receiver coils coupled to a dc SQUID preamplifier in a small scale magnetic resonance imaging (MRI) system operating at 425 kHz (B/sub 0/=0.01 T). The coil and SQUID are cooled to 4.2 K in a modified biomagnetic cryostat. The modifications provide transparency to rf signals originating outside the cryostat while maintaining an acceptably low liquid helium boiloff rate. By applying negative feedback we have damped low loss, high Q-factor SQUID input circuits to achieve a useful imaging bandwidth of over 2 kHz.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Immune response and resistance to Rous sarcoma virus challenge of chickens immunized with cell-associated glycoproteins provided with a recombinant avian leukosis virus (1991)

The Rous-associated virus 1 env gene, which encodes the envelope gp85 and gp37 glycoproteins, was isolated and inserted in place of the v-erbB oncogene into an avian erythroblastosis virus-based vector, carrying the neo resistance gene substituted for the v-erbA oncogene, to generate the pNEA recombinant vector. A helper-free virus stock of the pNEA vector was produced on an avian transcomplementing cell line and used to infect primary chicken embryo fibroblasts (CEFs) or quail QT6 cells. These infected cells, selected with G418 (CEF/NEA and QT6/NEA, respectively) were found to be resistant to superinfections with subgroup A retroviruses. The CEF/NEA preparations were used as a cell-associated antigen to inoculate adult chickens by the intravenous route compared with direct inoculations of NEA recombinant helper-free virus used as a cell-free antigen. Chickens injected with the cell-associated antigen (CEF/NEA) exhibited an immune response demonstrated by induction of high titers of neutralizing antibodies and were found to be protected against tumor production after Rous sarcoma virus A challenge. Conversely, no immune response and no protection against Rous sarcoma virus A challenge were observed in chickens directly inoculated with cell-free NEA recombinant virus or in sham-inoculated chickens.
Three novel antimicrobial peptides from the skin of the Indian bronzed frog <i>Hylarana temporalis</i> (Anura: Ranidae) (2011)

Amphibian skin secretion is considered as a rich source of bioactive peptides. The present work describes the successful identification of three novel peptides named brevinin-1TEa, brevinin-2TEa and brevinin-2TEb present in the skin secretion of Indian bronzed frog Hylarana temporalis. The deduced open reading frame encoding the biosynthetic precursor of brevinin-1TEa consisted of 70 amino acid residues and brevinin-2TEa and brevinin-2TEb consisted of 71 and 72 amino acids, respectively. All the three peptides showed higher antimicrobial activity against Gram-negative than against Gram-positive bacteria. On the basis of the antibacterial and haemolytic activity, brevinin-2TEb is the most potent peptide reported in the present study. Further research on these peptides may provide potential clue towards newer drug development to combat various microbial diseases.
Estimation of crop water requirement, effective rainfall and irrigation water requirement for vegetable crops using CROPWAT (2015)

Land and water resources are the basic needs of agriculture and for the economic development of any country. As water becomes increasing scare and increasing demand for irrigation water, everywhere, has now focused national attention and public interest on utilization of existing water supplies, integrated irrigation water conservation and management policy and practices. Accurate quantification of evapotranspiration is crucial for better management and allocation of water resources. It is important that the water requirements of crops are known at different management levels within the irrigated area to accomplish effective irrigation management. Estimation of the evapotranspiration and effective rainfall are extremely useful for operation planning and management issues. CROPWAT is a computer model, was used to estimate the reference evapotranspiration, effective rainfall, crop water requirement and irrigation water requirement for the Bapatla region in Andhra Pradesh state, India. Daily meteorological data including rainfall, maximum and minimum temperatures, relative humidity, wind speed and sunshine hours were collected for the period of 2009 to 2013 and used as input data for CROPWAT. Average peak monthly ET 0 was observed to be 8.09 mm/day for the month of June and followed by the 7.55 mm/ day for the month of May. Whereas average minimum ET 0 were observed as 3.85 and 3.92 mm/day in the months of December and January, respectively. The average effective rainfall was estimated for the study area as 769.3 mm out of 1060.3 mm annual rainfall. The crop water requirement (ETc) and irrigation water requirement were estimated for vegetable crops during Rabi season in the study area (Bapatla) as 516.3 mm and 470.4 mm, respectively.
Regulation of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Promoter by Nuclear Receptors Liver Receptor Homologue-1 and Small Heterodimer Partner (2005)

Cholesterol homeostasis in mammals involves pathways for biosynthesis, cellular uptake, and hepatic conversion to bile acids. Key genes for all three pathways are regulated by negative feedback control. Uptake and biosynthesis are directly regulated by cholesterol through its inhibition of the proteolytic activation of the sterol regulatory element binding proteins. The conversion of cholesterol into bile acids in the liver is regulated through the bile acid-dependent induction of the negatively acting small heterodimer partner nuclear receptor. In this report, we have shown that the small heterodimer partner also directly regulates cholesterol biosynthesis through inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase but has no effect on low density lipoprotein receptor expression. This has significant metabolic significance, as it provides both a mechanism to independently regulate cholesterol synthesis from uptake (an essential regulatory feature known to occur in vivo) and a pathway for direct regulation of cholesterol biosynthesis by bile acids. This latter feature ensures that the early phase of bile acid synthesis (pre-cholesterol) is in metabolic communication with the later stages of the pathway to properly regulate whole pathway flux. This highlights an important regulatory feature that is shared with other key branched, multienzyme pathways, such as glycolysis, where pathway outflow through pyruvate kinase is regulated by the concentration of a key early intermediate, fructose 1,6-bisphosphate.
A Role for the Cytoskeleton-associated Protein Palladin in Neurite Outgrowth (2001)

The outgrowth of neurites is a critical step in neuronal maturation, and it is well established that the actin cytoskeleton is involved in this process. Investigators from our laboratory recently described a novel protein named palladin, which has been shown to play an essential role in organizing the actin cytoskeleton in cultured fibroblasts. We investigated the expression of palladin in the developing rat brain by Western blot and found that the E18 brain contained a unique variant of palladin that is significantly smaller (∼85 kDa) than the common form found in other developing tissues (90–92 kDa). Because the expression of a tissue-specific isoform suggests the possibility of a cell type-specific function, we investigated the localization and function of palladin in cultured cortical neurons. Palladin was found preferentially targeted to the developing axon but not the dendrites and was strongly localized to the axonal growth cone. When palladin expression was attenuated by transfection with antisense constructs in both the B35 neuroblastoma cell line and in primary cortical neurons, a reduction in the expression of palladin resulted in a failure of neurite outgrowth. These results implicate palladin as a critical component of the developing nervous system, with an important role in axonal extension.
Fusing camera and Wi-Fi sensors for opportunistic localization (2011)

There has been a lot of research done towards both camera and Wi-Fi tracking respectively, both these techniques have their benefits and drawbacks. By combining these technologies, it is possible to eliminate their respective weaknesses, to increase the possibilities of the system as a whole. This is accomplished by fusing the data from Wi-Fi and camera before inserting it in a particle filter. This will result in a more accurate and robust localization system. The measurement model for Wi-Fi data uses a difference feature vector for comparing data to the fingerprint. The images taken from the camera are analysed, and filtered to detect human shapes. In this paper it is proven that an increased accuracy can be achieved by fusing the sensor data of both Wi-Fi and camera.
Identification of Protein Vaccine Candidates from <i>Helicobacter p</i><i>ylori</i> Using a Preparative Two-Dimensional Electrophoretic Procedure and Mass Spectrometry (2000)

Helicobacter pylori is an important human gastric pathogen for which the entire genome sequence is known. This microorganism displays a uniquely complex pattern of binding to complex carbohydrates presented on host mucosal surfaces and other tissues, through adhesion molecules (adhesins) on the microbial cell surface. Adhesins and other membrane-associated proteins are important targets for vaccine development. The identification and characterization of cell-surface proteins expressed by H. pylori is a prerequisite for the development of vaccines designed to interfere with bacterial colonization of host tissues. However, identification of membrane proteins is difficult using a traditional proteomics approach employing 2D-PAGE. We have used a novel approach in the identification of microbial proteins that employs a rapid preparative two-dimensional electrophoretic separation followed by mass spectrometry and database searches. No pre-enrichment of bacterial membranes was required. The entire process, from sample preparation to protein identification, can be completed in less than 18 hours, and the presence of proteins can be monitored after both the first- and second-dimensional separations using mass spectrometry. We were able to identify 40 proteins from a detergent-solubilized H. pylori preparation; over one-third of these were membrane or membrane-associated proteins. A functionally characterized low-abundance membrane protein, the Leb-binding adhesin, was found in this group. The use of this rapid 2D electrophoretic separation in proteomic studies of H. pylori is expected to speed up the identification of expressed virulence proteins and vaccine targets in this and other microbial pathogens.
DbpA, but Not OspA, Is Expressed by <i>Borrelia burgdorferi</i> during Spirochetemia and Is a Target for Protective Antibodies (1998)

DbpA is a target for antibodies that protect mice against infection by cultured Borrelia burgdorferi. Infected mice exhibit early and sustained humoral responses to DbpA and DbpB, suggesting that these proteins are expressed in vivo. Many antigens expressed in mammals by B. burgdorferi are repressed in vitro at lower growth temperatures, and we have now extended these observations to include DbpA and DbpB. To confirm that the protective antigen DbpA is expressed in vivo and to address the question of its accessibility to antibodies during infection, we examined B. burgdorferi in blood samples from mice following cutaneous inoculation. B. burgdorferi was visualized by dark-field microscopy in plasma samples from spirochetemic mice, and an indirect immunofluorescence assay showed that these spirochetes were DbpA positive and OspA negative. We developed an ex vivo borreliacidal assay to show that hyperimmune antiserum against DbpA, but not OspA, killed these plasma-derived spirochetes, demonstrating that DbpA is accessible to antibodies during this phase of infection. Blood transferred from spirochetemic donor mice readily established B. burgdorferi infection in naive recipient mice or mice hyperimmunized with OspA, while mice hyperimmunized with DbpA showed significant protection against challenge with host-adapted spirochetes. Antiserum from persistently infected mice had borreliacidal activity against both cultured and plasma-derived spirochetes, and adsorption of this serum with DbpA substantially depleted this killing activity. Our observations show that immunization with DbpA blocks B. burgdorferi dissemination from the site of cutaneous inoculation and suggest that DbpA antibodies may contribute to control of persistent infection.
Distributing the power of a government to enhance the privacy of voters (1986)

Article Distributing the power of a government to enhance the privacy of voters Share on Authors: Josh C Benaloh Yale University Yale UniversityView Profile , Moti Yung Columbia University Columbia UniversityView Profile Authors Info & Claims PODC '86: Proceedings of the fifth annual ACM symposium on Principles of distributed computingNovember 1986 Pages 52–62https://doi.org/10.1145/10590.10595Online:01 November 1986Publication History 138citation559DownloadsMetricsTotal Citations138Total Downloads559Last 12 Months23Last 6 weeks5 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access