gebhart/patrepeval huggingface dataset:
Methylation of membrane proteins in human erythrocytes. Identification and characterization of polypeptides methylated in lysed cells. (1981)

An in vitro system was developed for studying protein methylation reactions in human red blood cells. Packed erythrocytes were lysed by freeze-thawing in the presence of S-adenosyl[methyl-3H]methionine. Specific incorporation of base-labile methyl groups into the band 3 anion transport protein and the major sialoglycoprotein (glycophorin, periodic acid-Schiff reagent-1) was demonstrated by dodecyl sulfate gel electrophoresis at pH 2.4, selective extractions with Triton X-100 and lithium diiodosalicylate, and protease sensitivity. Two other unidentified intrinsic membrane proteins with Mr = 96,000 and 23,500 were also methylated. Little radioactivity was incorporated into membrane proteins when membranes were incubated with S-adenosyl-L-[methyl-3H]methionine in the absence of cytosol. No evidence was obtained for incorporation of methyl label into extrinsic proteins including bands 1, 2, 2.1, 4, 5, 6, or in zone 4.5. Proteolytic digestion of intact cells and isolated membranes revealed that one site of methylation on the band 3 polypeptide may be at the inner surface of the membrane near the junction of the cytoplasmic domain and the membrane domain. The rates of hydrolysis of the incorporated methyl groups were characterized over a range of pH values. These rates were compared to those of methyl esterified amino acids and peptides, including aspartic acid beta-methyl ester which has been isolated from proteolytic digests of methylated erythrocyte membranes (Janson, C. A., and Clarke, S. (1980) J. Biol. Chem. 225, 11640-11643). We find that the rates of base-catalyzed hydrolysis of beta-methyl esters of aspartic acid and gamma-methyl esters of glutamic acid are highly sensitive to the presence of substituents on the alpha-carboxyl and alpha-amino groups. The rate of hydrolysis of the membrane-incorporated methyl groups are consistent with those of internal aspartic acid and glutamic acid methyl ester residues.
Performance of Cage Floating Breakwater (1997)

Conventional floating breakwaters are examined and the feasibility of developing a cage floating breakwater is explored. Earlier studies on floating breakwaters reveal that, to achieve a transmission coefficient (Kt) less than 0.5, the breakwater width to wave length ratio (W/L) should necessarily be greater than 0.4 for most of the configurations. Recent studies on cost-effective floating breakwaters indicate that by fixing a row of pipes below the floating body, the W/L requirement can be reduced to 0.15 without any compromise in the performance. This concept has been adopted in developing a new configuration to serve as: (1) a floating breakwater; and (2) as a possible shallow water cage culture unit. Experiments were conducted to study the performance of the cage floating breakwater under wave and wave-current environment. The results on transmission and reflection coefficients are presented and compared with those reported in the literature. The variation of water surface oscillations and velocities within the cage, the effect of mooring line stiffness, and initial tension on transmission characteristics are also discussed.
The Evolutionarily Conserved Kaposi's Sarcoma-associated Herpesvirus ORF57 Protein Interacts with REF Protein and Acts as an RNA Export Factor (2004)

ORF57 (MTA) one of the earliest Kaposi's sarcoma-associated herpesvirus (KSHV) regulatory proteins to be expressed is essential for virus lytic replication. A counterpart is present in every herpesvirus sequenced, indicating the importance of this signature viral protein and those examined act post-transcriptionally, affecting RNA splicing and transport. In KSHV-infected cells, ORF57 protein was present in a complex with REF (Aly) and TAP (NXF1), factors involved in cellular mRNA export. The ORF57 N-terminal region interacts with REF, whereas both N- and C-terminal domains of REF interact with ORF57. The ORF57-REF interaction was direct, whereas TAP appeared to be recruited via REF. In somatic cells, ectopically expressed ORF57 protein was shown to function as a CRM1-independent nuclear mRNA export factor, promoting export of mRNAs that are poor substrates for splicing. The gamma-herpesvirus ORF57 protein, and its alpha-1 herpesvirus ICP27 counterpart both export RNA through pathways involving REF and TAP proteins, although divergence of these herpesvirus subfamilies occurred some 180-210 million years ago. The TAP-mediated cellular mRNA export pathway is CRM1-independent. However, human immunodeficiency virus type 1 Rev protein-mediated RNA export, which is CRM1-dependent, was considerably inhibited by ORF57, suggesting that Rev and ORF57 compete for a common export component. These data strengthen arguments that TAP and CRM1 pathways converge in accessing similar components of the nuclear pore complex. We propose that ORF57-mediated RNA export may use different export factors to accommodate the KSHV-infected host cell environments, for example, in B-cells or endothelial cells and during the different phases of lytic virus replication.
Screening for gene mutations: will identification of<i>NT5C2</i>mutations help predict the chance of relapse in acute lymphoblastic leukemia? (2013)

"Screening for gene mutations: will identification of NT5C2 mutations help predict the chance of relapse in acute lymphoblastic leukemia?." Expert Review of Hematology, 6(3), pp. 223–224
Tyrosine phosphorylation and association of Syk with Fc<i>γ</i>RII in monocytic THP-1 cells (1995)

Although the cytoplasmic portion of the low-affinity receptor for immunoglobulin G, Fc gamma RII, does not contain a kinase domain, rapid tyrosine phosphorylation of intracellular substrates occurs in response to aggregation of the receptor. The use of specific tyrosine kinase inhibitors has suggested that these phosphorylations are required for subsequent cellular responses. We previously demonstrated the coprecipitation of a tyrosine kinase activity with Fc gamma RII, suggesting that non-receptor tyrosine kinases might associate with the cytoplasmic domain of Fc gamma RII. Anti-receptor immune complex kinase assays revealed the coprecipitation of several phosphoproteins, most notably p56/53lyn, an Src-family protein tyrosine kinase (PTK), and a 72 kDa phosphoprotein. Here we identify the 72 kDa Fc gamma RII-associated protein as p72syk (Syk), a member of a newly described family of non-receptor PTKs. A rapid and transient tyrosine phosphorylation of Syk was observed following Fc gamma RII activation. Syk was also tyrosyl-phosphorylated following aggregation of the high-affinity Fc gamma receptor, Fc gamma RI. The Fc gamma RI activation did not result in association of Syk with Fc gamma RII, implying that distinct pools of Syk are activated upon aggregation of each receptor in a localized manner. These results demonstrate a physical association between Syk and Fc gamma RII and suggest that the molecules involved in Fc gamma RII signalling are very similar to the ones utilized by multichain immune recognition receptors such as the B-cell antigen receptor and the high-affinity IgE receptor.
Science and the campaigners (2000)

Campaigners routinely use and abuse ‘science’ in their arguments, while often complaining about the ‘reductionist’ basis of science, and society's over‐reliance on scientific arguments. This paper examines the long‐running battle between old‐fashioned ideas derived from the Enlightenment and the newer ‘Post‐Modern’ and Romantic ideas now used by campaigners.
Discontinuous and Mixed Finite Elements for Two-Phase Incompressible Flow (1990)

Summary The simulation of multiphase flow presents several difficulties, including (1) the occurrence of sharp moving fronts when convection is dominating, (2) the need for a good approximation of velocities to calculate the convective terms of the equation, and (3) flow singularities around wells. To handle the first difficulty, we propose a Godunov-type higher-order scheme based on a piecewise linear approximation of the saturation associated with a multidimensional slope limiter. With respect to the second, the pressure equation is approximated by means of a mixed-hybrid formulation equivalent to the classic mixed formulation but yielding a positive-definite linear system. To solve the third difficulty, we introduce macroelements around wells. Numerical experiments illustrate the capabilities of the method.
A highly sensitive and specific assay for determination of IGF-I bioactivity in human serum (2003)

At present, the circulating bioactivity of insulin-like growth factor I (IGF-I) is estimated by immunological measurements of IGF-I levels. However, immunoassays ignore the modifying effects of the IGF-binding proteins (IGFBPs) on the interaction between IGF-I and the IGF-I receptor (IGF-IR). Therefore, we developed an IGF-I kinase receptor activation assay (KIRA) based on cells transfected with the human IGF-IR gene. The bioassay was sensitive (detection limit 0.08 microg/l), specific (cross-reactivity of insulin, insulin analogs, and proinsulin was <1%; IGF-II cross-reactivity was 12%), and accurate (within- and between-assay coefficients of variation <7 and <15%). The operational range of the assay (0.25-10.0 microg/l) allowed for determination of IGF-I bioactivity in serum from patients with, for example, growth hormone deficiency, type 1 diabetes, and acromegaly. Addition of IGFBPs dose dependently reduced the KIRA signal, whereas addition of IGF-II to preformed complexes (1:1 molar ratio) of IGF-I and IGFBP dose dependently increased IGF-I bioactivity by displacement of bound IGF-I. In conclusion, the KIRA will enable us to compare IGF-I bioactivity with existing immunological measurements of IGF-I in serum and, hopefully, to elucidate the factors that determine IGF-I bioactivity in vivo.
Linear-Time Approximation for Maximum Weight Matching (2014)

The maximum cardinality and maximum weight matching problems can be solved in Õ ( m √ n ) time, a bound that has resisted improvement despite decades of research. (Here m and n are the number of edges and vertices.) In this article, we demonstrate that this “ m √ n barrier” can be bypassed by approximation. For any ε > 0, we give an algorithm that computes a (1 − ε )-approximate maximum weight matching in O ( mε −1 log ε −1 ) time, that is, optimal linear time for any fixed ε . Our algorithm is dramatically simpler than the best exact maximum weight matching algorithms on general graphs and should be appealing in all applications that can tolerate a negligible relative error.
Isolated regional perfusion in the treatment of malignant melanomas of the extremities. (1975)

The authors present a survey of changed views on the treatment of patients with malignant melanoma of the extremities. In addition to conventional therapy, regional perfusion is discussed. Over the period 1964-1970, 115 perfusions were performed in 75 patients. The perfusions were confined to patients with a tumor infiltrating beyond the stratum papillare. The 4-year survival of 30 patients with a primary tumor, without demonstrable metastases at the moment of perfusion, was found to be 77%. The percentage of local recurrences was significantly lower after perfusion than after conventional surgery.
Smart Device Control Using Augmented Reality (2015)

In recent years, augmented reality technology is being widely used in various areas. Augmented Reality (AR) is basically a real view modified by a computer. It uses computer generated imagery and sounds to enhance one's current perception of reality. While virtual reality describes an imaginary world that exists in computers and our minds, augmented reality is a mixture of real world and virtual world. It is therefore different for the human mind to tell the difference between real world and augmented reality. In this paper, we describe the development of a prototype that uses augmented reality to control electrical devices like fan, motors, AC etc by hand gestures. Up to 8 electrical devices can be controlled. A virtual plane is present between user and display. The user interacts with this virtual plane and hands and fingers are tracked and gestures recognized. These gestures are then used to select the button on the display which are used for selecting a particular device and to turn it on or off as per the requirement of user. This project is very helpful for people of physical limitations like old age people, physically disabled people and also used for industrial and office work.
Politics, legislation and archaeology: A New South Wales case study (1996)

This thesis examined the political influences on protective legislation of archaeological resources. Pluralist theory was borrowed from political science to produce a critique on the changes by examining the reasons behind these changes. Through identification and comparison of 'pressure groups', how and why the legislation changed was shown. The National Parks and Wildlife Act 1974 (NSW) was used as a case study to historically analyse the influence of pressure groups in New South Wales. My analysis allows archaeologists to develop a political perspective on legislative change and participate more effectively in the legislative process. The proposed amendments to the National Parks and Wildlife Act reflect that social perspectives are changing. The National Parks and Wildlife (Aboriginal Ownership) bills have purported to give Aboriginal communities ownership of culturally significant areas. To date, these amendments have had trouble being passed. Such legislation, once passed, will undoubtedly have an impact on archaeology and cultural resource management in Australia Significantly, if the bills are left unpassed, this impact will be considerably greater. It was concluded that the National Park and Wildlife Act had developed in three chronological stages. From these it was seen that Aboriginal pressure groups were by far the most influential in initiating change. Among these changes, the most important was that the 'universality' of Aboriginal cultural heritage is being broadened. Archaeologists were left in a reactive role to all changes. Â
Collaborative real-time speaker identification for wearable systems (2010)

We present an unsupervised speaker identification system for personal annotations of conversations and meetings. The system dynamically learns new speakers and recognizes already known speakers using one audio channel and speech-independent modeling. Multiple personal systems could collaborate in robust unsupervised speaker identification and online learning. The system was optimized for real-time operation on a DSP system that can be worn during daily activities. The system was evaluated on the freely available 24-speaker Augmented Multiparty Interaction dataset. For 5 s recognition time, the system achieves 81% recognition rate. Collaboration between four identification systems resulted in a performance increase of up to 17%, however even two collaborating systems yield an performance improvement. A prototypical wearable DSP implementation could continuously operate for more than 8 hours from a 4.1 Ah battery.
WeChat Applet Promotion Strategies of Small Shops (2020)

Since being launched in 2017, the WeChat applet (or WeChat mini-program) has won wide popularity and given rise to a new field of online marketing by dint of its convenient loading functions and support from WeChat's large user base. WeChat applets have greatly facilitated connection between online and offline business, and revolutionary changes to small brick-and-mortar stores in particular. Compared with small and medium-sized stores, small stores know little about promotion on WeChat applets. Case studies on applet promotion of several small stores revealed that traditional website promotion strategies and app promotion strategies are not applicable to WeChat applet promotion. To promote small stores on WeChat applets, multiple promotion strategies including online, offline and free promotion strategies should be combined, and the customers' social network should be taken as the key point of network promotion. This paper makes an in-depth analysis of the promotion strategies of small stores on WeChat applets.
<title>High-speed electrically controlled GaAs quantum well spatial light modulators: device creation and applications</title> (1998)

GaAs asymmetric Fabry-Perot vertical cavity modulators are useful in a wide variety of applications. Vertical cavity devices have employed amplitude or phase modulation for optical switching. Amplitude modulators have been demonstrated in large format arrays. Additional uses for the pixels include directional modulation, detection, and light- emitting capability. When arrays of these deices are integrated with electronic circuits - most significantly silicon CMOS VLSI - at the pixel level, large, complex optical spatial light modulators, detectors, transceivers, computation devices, and emitters can be created for a wide variety of applications. These applications range from target recognition to SAR radar processing, to optical data routing, to optical interconnect systems, to optical memory access.
骨髓自家融解液の骨髓造血に及ぼす影響 第1編 腹腔内注射並に骨髄灌流実験 (1955)

The author, as a link in the serial study on improvement of medullary hematopoiesis by so-called bone-marrow substance, tried to investigate both effects and functions of parenteral administration of rabbit's red bone-marrow autolysate (called simply as "bone-marrow extract" hereafter); so that, having injected its certain amount into normal rabbit's abdominal cavity, the author inspected its hourly effect on peripheral blood picture as well as on blood picture of V. nuturitica femoralis, and also took observations of certain changes that occurred to intramedullary circulation. As a result, the author discovered that: 1) the biphasic blood cell increase was induced in both cases of single and successive injection alike; 2) in its secondary blood cell increase, the increase was more remarkable in case of successive, than in case of single, injection; 3) above all, an increase of reticulocytes, a comparative increase of pseudoeosinocytes, and a left shift of leucocytes, were remarkably induced. Moreover, in case of the primary blood cell increase, an expansion of medullary nutric vessels, a marked blood cell increase in nutric veinal blood, and a comparative increase of pseudoeosinocytes --- i. e. a liberation of accumulated blood cells within bone-marrow --- were brought about; and further, the blood stream within bone-marrow under the two phases of blood cell increase was noticed to be remarkably stimulated, as compared with the case in normal rabbit. From the above results, it may roughly be said that, in case the bone-marrow extract should be intraabdominally injected, a biphasic blood cell increase occures in both cases of single and successive injection, the latter being more powerful in stimulating medullary hematopoiesis; and that the primary blood cell increase is caused by the mobilization as well as the liberation of accumulated blood cells which is due to the stimulation to blood stream within bone-marrow, while the secondary blood cell increase owes much to the hematopoietic function of bone-marrow which is originated in the continuance of stimulation to blood stream within bone-marrow.
Gut microbiome: pertinence in fibromyalgia. (2020)

The human gut microbiome constitutes a diverse and dynamic community of microorganisms that inhabit the digestive tract. In recent years, there is growing appreciation for the role of the gut microbiome in host health and disease. Gut bacteria are involved in the pathogenesis of numerous medical conditions in a variety of medical fields including gastroenterology, metabolic, rheumatologic, neurologic and psychiatric disorders. Recently, evidence is mounting that gut bacteria could also play a role in chronic pain and specifically fibromyalgia (FM). The composition of the gut bacterial community is altered in individuals with FM, with an altered abundance of a small subset of bacterial species. Some of these species, either with increased or decreased abundance in patients, have established metabolic activity which could have pertinence in the expression of FM symptoms. The putative mechanisms which could allow these bacterial species to affect pain, fatigue, mood and other symptoms include the entry of short-chain-fatty-acids, bile acids, neurotransmitters and bacterial antigens into the host circulation. While these are merely the first steps in understanding the role of the gut microbiome in chronic pain and specifically FM, one might envision exciting future perspectives for better mechanistic understanding of FM, for the development of objective diagnostic aids and potentially for new therapeutic modalities.
Ray tracing NPR-style feature lines (2008)

Though the goal of ray tracing and other physically based rendering techniques is ultimately to produce photorealistic images, it is often helpful to use non-photorealistic rendering techniques to illustrate or highlight certain features in a rendering. We present a method for ray tracing constant screen-width NPR-style feature lines on top of regularly rendered scenes, demonstrating how a variant of line rasterization can be included in a ray tracer, thus allowing for the inclusion of NPR-style enhancements. We are able to render silhouette edges, marking the boundary of an object in screen space against the background (or against farther parts of the same object), intersection lines, marking the curves along which two primitives intersect, and crease edges, indicating curves along which a primitivepsilas normal field is dicontinuous. Including these lines gives the viewer an additional cue to relative positions of objects within the scene, and also enhances particular features within objects, such as sharp corners. The method in this paper was developed in particular for enhancing glyph-based scientific visualization; however, the basic technique can be adapted for many illustrative purposes in different settings.
Tracking Conflicting Accesses Efficiently for Software Record and Replay (2012)

Record and replay, which records a multithreaded program’s execution in one run and reproduces it deterministically in a second run, is useful for program debugging, fault detection and analysis. The key challenge in multithreaded record and replay is ensuring that conflicting, cross-thread accesses to shared variables are properly detected, recorded and reproduced. Numerous solutions have been proposed in both hardware and software to track these cross-thread accesses, but to date all general-purpose software solutions suffer from high overhead or have serious limitations. This paper introduces ROCTET, an approach for performing softwareonly deterministic record and replay. ROCTET is built on top of a novel dynamic analysis infrastructure, OCTET, which can detect at low overhead any cross-thread dependences during execution by exploiting the fact that most accesses, even of shared objects, are not part of cross-thread dependences. We implement OCTET and ROCTET in a JVM and show they add low overhead for several multithreaded applications. We also show that our implementation of ROCTET can successfully replay recorded executions when it can successfully control or ignore extraneous sources of nondeterminism in the VM, libraries, and system.