gebhart/patrepeval huggingface dataset:
Construction and application of chimeric virus-like particles of tick-borne encephalitis virus and mosquito-borne Japanese encephalitis virus (2007)

We have previously reported a system for packaging tick-borne encephalitis (TBE) virus subgenomic replicon RNAs into single-round infectious virus-like particles (VLPs) by using in trans expression of viral C/prM/E structural proteins. In this study, the trans-packaging system was applied to the generation of chimeric VLPs with mosquito-borne Japanese encephalitis (JE) virus. Although trans-expression of TBE virus C and JE virus prM/E proteins resulted in the secretion of VLPs, the expression of JE virus C/prM/E proteins did not lead to the secretion of VLPs, suggesting that homologous interaction between C and non-structural proteins or the genomic RNA is important for efficient assembly of infectious particles. Neutralization testing showed that the antigenic characteristics of the VLPs were similar to those of the native virus. Furthermore, the infectivities of the TBE virus- and JE virus-enveloped VLPs for the ISE6 tick cell line and C6/36 mosquito cell line were investigated. The VLPs were able to enter only those cells that were derived from the natural vectors for the respective viruses. TBE virus replicon RNA packaged in VLPs produced TBE virus non-structural proteins in tick cells, but could neither replicate nor produce viral proteins in mosquito cells. These findings indicate the importance of specific cellular factors for virus entry and replication during flavivirus infection of arthropods. These results demonstrate that chimeric VLPs are useful tools for the study of viral genome packaging and cellular factors involved in vector specificity, with the additional safety aspect that these chimeric VLPs can be used instead of full-length chimeric viruses.
The Receptor Binding Site for the Methyltransferase of Bacterial Chemotaxis Is Distinct from the Sites of Methylation (1996)

The principal locus for binding interactions between the aspartate and serine receptors of escherichia coli and the methyltransferase was found to be in the last five amino acids of the receptor. The thermodynamic parameters of transferase-receptor interactions were determined by isothermal titration calorimetry. the serine receptor and three C-terminal fragments (C-fragments) of the aspartate receptor consisting of ether the last 297, 88, or 38 amino acids gave comparable values for binding (n=1, deltaH approximately 13 kcal/mol, and Ka approximately 4 x 10(5)M-1). Truncating either 16 or 36 amino acids form the C-terminus eliminated observable interactions. Finally the pentapeptide Asn-Trp-Glu-Thr-Phe, which corresponds to the last five amino acids of the receptor and is strictly conserved among E. coli serine amd aspartate receptors and the Salmonella typhimurium aspartate receptor, was found to have all the binding activity of the full-length receptor and the C-fragments. An in vitro methylation assay was used to obtain evidence for the physiological significance of this interaction in which excess peptide was able to completely block receptor methylation. The location of the binding site far from the methylation sites in the primary structure of the receptor suggests that the principle role of this interaction may be to hold the transferase in close proximity to all the methylation sites. Intersubunit methylation implication is proposed as plausible consequence of this "controlled proximity" mechanism since the ribose-galactose and dipeptide receptors lack the transferase binding sequence, and appear unable to bind transferase. Intersubunit methylation implies that transferase bound to eother the serine or aspartate receptor subunit may catalyze methylation of receptor subunits in a neighboring dimer, including those that have ligand specificity.
Performance evaluation of distributed compressed wideband sensing for cognitive radio networks (2008)

In emerging cognitive radio (CR) networks, the first cognitive task preceding any dynamic spectrum management is the sensing and identification of spectrum holes in wireless environments. This paper develops a distributed compressed spectrum sensing approach for (ultra-)wideband CR networks. First, compressive sampling is performed at local CRs to scan the very wide spectrum at practical signal-acquisition complexity. Then, measurements or states from multiple CR detectors are fused to collect spatial diversity gain, which improves the detection quality especially under fading channels. Distributed fusion algorithms are derived to effect collaborative fusion at low communication and computation load scalable to the network size. Multiple performance metrics are identified to evaluate the proposed spectrum sensing techniques, as well as to assess those performance-determining factors in system design. Computer simulations corroborate the effectiveness of the proposed techniques in identifying and locating the spectrum hole opportunities in wireless fading environments.
Androgens and masculinization of genitalia in the spotted hyaena ( <i>Crocuta crocuta</i> ). 2. Effects of prenatal anti-androgens (1998)

Pregnant spotted hyaenas were treated with anti-androgens to interfere with the unusually masculine 'phallic' development that characterizes females of this species. The effects on genital morphology and plasma androgen concentrations of infants were studied during the first 6 months of life. Although there were consistent 'feminizing' effects of prenatal anti-androgen treatment on genital morphology in both sexes, such exposure did not produce males with extreme hypospadia, as it does in other species, nor did it produce females with a 'typical' mammalian clitoris and external vagina. 'Feminization' of males resulted in a penis with the morphological features of the hyaena clitoris, and 'feminization' of females exaggerated the sex differences that are typical of this species. The effects of treatment were present at birth and persisted for at least 6 months. Treatment of pregnant females with flutamide and finasteride also markedly reduced circulating concentrations of testosterone and dihydrotestosterone in maternal plasma during pregnancy. Plasma delta 4-androstenedione was reduced in the female, but not the male, infants of treated mothers, consistent with an epigenetic hypothesis previously advanced to explain hormonal 'masculinization' of females. The present 'feminizing' effects of prenatal anti-androgen treatment are consistent with contemporary understanding of sexual differentiation, which accounts for morphological variation between the sexes in terms of steroids. However, current theory does not account for the basic genital structure of females and the present data suggest that development of the male penis and scrotum, and the female clitoris and pseudoscrotum, in spotted hyaenas may involve both androgen-dependent and androgen-independent components.
[The effect of specific immunization and BCG vaccine on the growth of Krebs-2 ascitic tumor in mice]. (1976)

Immunization of CC57Br mice with irradiated Krebs-2 carcinoma cells renders no influence on the rate of ascites aggravation in subsequent transplantation of the tumor but increases considerably life-terms of tumor-bearing animals. Immunization with tumor cells in combination with not high doses of BCG results in a reverse development of ascites in some mice and a complete disappearance of tumor in them. With increased dosage of the vaccine the number of animals with ascites regression is increased, but their life-terms are reduced due to reactivation of the tumor process and, presumably, to the development of autoimmune lesions.
Developing therapeutic interview skills in medical students (1991)

There has been considerable interest in doctors' interviewing skills during the last few decades (Nuffield Working Party, 1978). Despite this interest, there has been no consensus as to how these skills may be taught to medical students. We describe the ongoing evolution of a teaching package for these skills as taught by the Liverpool University Department of Psychiatry.
Glucose oxidase electrodes of polyaniline, poly(<i>o</i>‐toluidine) and their copolymer as a biosensor: a comparative study (2004)

Abstract A simple technique is described for constructing a glucose sensor by the entrapment of glucose oxidase (GOD) in a polyaniline (PA), poly( o ‐toluidine) (POT) and their copolymer poly(aniline‐co‐ o ‐toluidine) (PA‐co‐POT) thin films, which were electrochemically deposited on a platinum plate in phosphate and acetate buffer. The maximum current response was observed for PA, POT, and PA‐co‐POT GOD electrodes at pH 5.5 and potential 0.60 V ( v . Ag/AgCl). The phosphate buffer gives fast response as compared to acetate buffer in amperometric measurements. PA GOD electrode shows the fastest response followed by PA‐co‐POT and POT GOD electrodes. Copyright © 2004 John Wiley & Sons, Ltd.
Comparison of automated and manual methods for islet isolation. (1990)

The authors used the principal features of a collagenase perfusion technique and an automated dissociation technique to determine if islets could be isolated from the large mammal pancreas and to compare the effects of the two methods on isolated islets. The pancreases of 16 dogs were cannulated and perfused with collagenase at 4 degrees C, then warmed to 37 degrees C. Group 1 (eight) pancreases were perfused at 37 degrees C until digested, then dissociated manually by teasing and trituration. Group 2 (eight) pancreases were transferred to a closed chamber for continued collagenase digestion and dissociation at 37 degrees C. Islets were purified using identical Ficoll gradients. Aliquots were stained with dithizone and evaluated for number, size and purity. Total islet volume was calculated. Group 2 pancreases were thoroughly digested leaving only a few residual ducts, but undigested fragments persisted in group 1 pancreases. Islet size was similar in both groups. There was a greater islet volume before and after Ficoll purification in group 2, but the difference was not significant. Purity was greater than 90% in both groups. Perifusion with 28 mM glucose elicited a biphasic insulin release from islets in both groups. The data show that the combined protocol enables mass isolation of purified islets from the canine pancreas. Compared with the manual technique, the automated protocol for pancreas dissociation tends to improve the yield of islets without compromising islet size and viability. It provides the advantages of a closed system with increased control over the extent of collagenase digestion.
Architecture and circuit techniques for low-throughput, energy-constrained systems across technology generations (2006)

Rising interest in the applications of wireless sensor networks has spurred research in the development of computing systems for low-throughput, energy-constrained applications. Unlike traditional performance oriented applications, sensor network nodes are primarily constrained by operation lifetime, which is limited by power consumption. Advanced CMOS process technologies provide ever increasing transistor density and improved performance characteristics. However, shrinking feature size and decreasing threshold voltages also lead to significant increases in leakage current, which is especially troublesome for applications with significant idle times. This work investigates tradeoffs between leakage and active power for low-throughput applications. We study these issues across a range of process technologies on a computing architecture that provides explicit support for fine-grain leakage-control techniques such as Vdd-gating and adaptive body bias. We present a methodology for selecting design parameters, including choice of process technology, that makes the optimal tradeoff between active power and leakage power for a given workload. Our results show that leakage power will dominate the selection of process technology, and architectures that support advanced leakage control techniques at the circuit level will be essential. We argue that without advanced low-power architectures future nano-scale process technologies will not be suited for sensor network applications.
Financial Economics (1997)

Abstract Financial Economics is an exciting new field of study that integrates the theory of finance and financial institutions into the main body of economic theory. In doing so, it draws on insights from general equilibrium analysis, information economics, and the theory of contracts. Financial Economics is a self-contained and comprehensive introduction to the field for advanced undergraduate and post-graduate economists and finance specialists. It develops the main ideas in finance theory, including the CAPM, arbitrage pricing, option pricing, and the Modigliani-Miller theorem within an economic framework. Students of economics are shown how finance theory derives from foundations in economic theory, while students of finance are given a firmer appreciation of the economic logic underlying their favourite results. Financial Economics provides all of the technical apparatus necessary to read the modern literature in financial economics and the economics of financial institutions. The book is self-contained in that the reader is guided through branches of the theory, as necessary, in order to understand the main topics. Numerous examples and diagrams illustrate the key arguments, and the main chapters are followed by guides to the relevant literature and exercises for students.
Diastereoselective Coupling Reaction of Activated Cyclic Ketene Ortho Ester with Aldehydes Promoted by Lewis Acid Catalyst (1997)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTDiastereoselective Coupling Reaction of Activated Cyclic Ketene Ortho Ester with Aldehydes Promoted by Lewis Acid CatalystChan-Mo Yu, Ha-Soon Choi, Jeon-Koo Lee, and Sook-Kyung YoonView Author Information Department of Chemistry, Sung Kyun Kwan University, Suwon 440-746, KoreaCite this: J. Org. Chem. 1997, 62, 19, 6687–6689Publication Date (Web):September 19, 1997Publication History Received1 May 1997Published online19 September 1997Published inissue 1 September 1997https://pubs.acs.org/doi/10.1021/jo9707747https://doi.org/10.1021/jo9707747brief-reportACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views223Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Aldehydes,Catalysts,Lewis acids,Mixtures,Organic compounds Get e-Alerts
On Thermonuclear War (1961)

On Thermonuclear War was controversial when originally published and remains so today. It is iconoclastic, crosses disciplinary boundaries, and finally it is calm and compellingly reasonable. The book was widely read on both sides of the Iron Curtain and the result was serious revision in both Western and Soviet strategy and doctrine. As a result, both sides were better able to avoid disaster during the Cold War. The strategic concepts still apply: defense, local animosities, and the usual balance-of-power issues are still very much with us. Kahn's stated purpose in writing this book was simply: "avoiding disaster and buying time, without specifying the use of this time." By the late 1950s, with both sides H-bomb-armed, reason and time were in short supply. Kahn, a military analyst at Rand since 1948, understood that a defense based only on thermonuclear arnaments was inconceivable, morally questionable, and not credible.The book was the first to make sense of nuclear weapons. Originally created from a series of lectures, it provides insight into how policymakers consider such issues. One may agree with Kahn or disagree with him on specific issues, but he clearly defined the terrain of the argument. He also looks at other weapons of mass destruction such as biological and chemical, and the history of their use. The Cold War is over, but the nuclear genie is out of the bottle, and the lessons and principles developed in On Thermonuclear War apply as much to today's China, Russia, Iran, and North Korea as they did to the Soviets.
I/Q multiplexed code assignment for fast cell search in asynchronous DS/CDMA cellular systems (1998)

For flexible system deployment, we consider asynchronous cell site operation which requires much longer cell search time than in synchronous operation. A new code assignment scheme is proposed to minimize the cell search time in which a group code is assigned to the in-phase pilot channel and a cell-specific code assigned to the quadrature pilot channel. Simulation results show that cell search can be accomplished in less than about 200 ms at 90% of the locations when the pilot code of length 1024 and eight group codes (64 cell-specific codes/group) are used.
Abstract 5627: Antitumor activity of soluble beta-1,3/1,6 glucans: Structure matters (2010)

Abstract Beta-glucan is a pathogen-associated molecular pattern recognized by a variety of innate immune cells. Beta-1,3/1,6 glucans possess numerous immune potentiating activities. In particular, antitumor activity has been demonstrated with the soluble beta glucan Imprime PGG used in combination with complement-activating antitumor monoclonal antibodies (MAb) in several tumor models. Structurally, beta-1,3/1,6 glucans can vary on the basis of chain length, types of linkages, branching, and tertiary structure. Previously, the antitumor activity of soluble beta-1,3/1,6 glucans in combination with antitumor MAb has only been reported using beta glucans derived from yeast. The primary objective of this study was to compare the antitumor activity of three structurally distinct soluble beta-1,3/1,6 glucans in vivo in a mouse syngeneic lymphoma model. Tumor-bearing mice were administered antitumor MAb plus beta glucan 2x/week for 4 weeks. Antitumor activity was based on inhibition of tumor growth at the tumor implantation site and enhanced long-term survival. In addition, critical innate immune cells responsible for antitumor activity were identified focusing on the role of neutrophils. Also, characterization of relevant human immune cells and their beta glucan receptors will be shown focusing on complement receptor 3 (CR3) and dectin-1. Beta glucan binding to immune cells and identification of relevant receptors were determined by flow cytometry using beta glucan-specific antibody and fluorescently-labeled beta glucans. Key findings are that soluble beta glucans, which differ structurally, do not induce similar antitumor activity, and antitumor activity is abrogated in granulocyte-deficient mice. Finally, soluble glucans bind to human neutrophils in a CR3-dependent manner. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5627.
Liquid metal embrittlement: A state-of-the-art appraisal (1999)

Although the risk of embrittlement of materials exposed to liquid metals has been recognized for many years, its prediction reMayns problematical insofar as the knowledge of the mechanisms involved in the phenomenon is limited. However, Liquid Metal Embrittlement (LME) is of prime interest because the risk of damage exists wherever the handling of liquid metals is required in various industrial or scientifical fields (chemical plants, power-producing systems, soldering process, ...). The interest for this phenomenon needs thus to be pursued. The present paper reviews experimental results about the occurrence of LME and the influence of different parameters, and presents a number of mechanisms which have been proposed to explain LME.
Cognitive computing in education (2016)

Cognitive computing is the new wave of Artificial Intelligence (AI), relying on traditional techniques based on expert systems and also exploiting statistics and mathematical model. In particular, cognitive computing systems can be regarded as a "more human" artificial intelligence. In fact, they mimic human reasoning methodologies, showing special capabilities in dealing with uncertainties and in solving problems that typically entail computation consuming processes. Moreover, they can evolve, exploiting the accumulated experience to learn from the past, both from errors and from successful findings. From a theoretical point of view, cognitive computing could replace existing calculators in many fields of application but hardware requirements are still high, even if the cloud infrastructure, which is expected to uphold its rapid growth in the very next future, can support their diffusion and ease the penetration of such a novel variety of systems, fostering new services as well as changes in many settled paradigms. In this paper, we focus on benefits that this technology can bring when applied in the education field and we make a short review of relevant experiences.
Quantitative structure-activity relationships in 1-aryl-2-(alkylamino)ethanol antimalarials (1979)

A quantitative structure-activity relationship has been formulated for 646 antimalarials acting against P. berghei in mice. The equation developed has 14 terms, 9 of which are indicator variables. The correlation coefficient for the QSAR is 0.898 and the standard deviation is 0.309. The antimalarials are all arylcarbinols of the type X-ArCHOHCH2NR1R2. Sixty different aryl structures, including a variety of heterocyles, are contained in the study. The most important determinate of activity is found to be the electron-withdrawing ability of the substituents X; the hydrophobic character of X and R plays less important roles. Suggestions for more potent analogues are made and the lack of activity of about 100 additional analogues is also considered.
Ultra low nonlinearity low loss pure silica core fiber for long-haul WDM transmission (1999)

A new design of pure silica core fiber with an effective area over 110 /spl mu/m/sup 2/ and attenuation of 0.171 dB/km at 1.55 /spl mu/m has been successfully developed. It exhibits ultra low nonlinearity and excellent bending loss performance.
Structure of the NS2B-NS3 protease from Zika virus after self-cleavage (2016)

The recent outbreak of Zika virus (ZIKV) infections in the Americas represents a serious threat to the global public health. The viral protease that processes viral polyproteins during infection appears as an attractive drug target. Here we report a crystal structure at 1.84 Å resolution of ZIKV non-structural protein NS2B-NS3 protease with the last four amino acids of the NS2B cofactor bound at the NS3 active site. This structure represents a post-proteolysis state of the enzyme during viral polyprotein processing and provides insights into peptide substrate recognition by the protease. Nuclear magnetic resonance (NMR) studies and protease activity assays unravel the protein dynamics upon binding the protease inhibitor BPTI in solution and confirm this finding. The structural and functional insights of the ZIKV protease presented here should advance our current understanding of flavivirus replication and accelerate structure-based antiviral drug discovery against ZIKV.