gebhart/patrepeval huggingface dataset:
Coordinate Expression of the PRM1, PRM2, and TNP2 Multigene Locus in Human Testis (1995)

Maintenance of the transcriptionally inert state of the mature human spermatozoon requires the expression of the various members of the human protamine gene cluster prior to the final stages of spermatogenesis. During this process, known as spermiogenesis, round spermatids morphologically differentiate into mature spermatozoa. The expression of the PRM1, PRM2, and TNP2 genes facilitates the compaction and condensation of the genetic material within the developing spermatid. To understand better the coordinate control governing this transformation, we have examined the localization and distribution of the human protamines PRM1 and PRM2 and transition protein TNP2 transcripts during human spermatogenesis. The stage-specific expression of these transcripts was determined by in situ hybridization analysis using [α-35S]-labeled cRNA probes. PRM1, PRM2, and TNP2 transcripts were abundant in association with round and elongating spermatids, located in the adluminal region of the seminiferous epithelium. They were not observed in association with spermatogonia, spermatocytes, Sertoli cells, or interstitial cells. These data indicate that the human PRM1, PRM2, and TNP2 transcripts are expressed postmeiotically in round and elongating spermatids. The quantitative evaluation of each transcript was determined as a function of the relative optical density per unit area. In all cases examined, the relative level of each transcript was consistent with the following pattern, PRM2 > PRM1 ≃ TNP2.
Pharmacokinetics, induction of anaesthesia and safety characteristics of Propofol 6% SAZN <i>vs</i> Propofol 1% SAZN and Diprivan<sup>®</sup>‐10 after bolus injection (1999)

From the above results, we conclude that the alteration of the type of emulsion and the higher concentration of propofol in the new parenteral formulation of propofol does not affect the pharmacokinetics and induction characteristics of propofol, compared with the currently available product. Propofol 6% SAZN can be administered safely and has the advantage of a reduction of the load of fat and emulsifier which may be preferable when long term administration of propofol is required.
Transforming growth factor‐β: An important mediator of immunoregulation (1991)

Transforming growth factor-beta (TGF-beta) is synthesized and secreted by a wide variety of cells, including cells of the immune system. Lymphocytes and monocytes possess high affinity TGF-beta receptors and the addition of TGF-beta to in vitro cell cultures results in significant modulation of immune function. TGF-beta inhibits the proliferation of thymocytes, T cells, B cells, and natural killer cells. Additionally, it inhibits certain differentiative functions of lymphocytes including a marked inhibition of immunoglobulin production by human B lymphocytes. TGF-beta has dichotomous actions on monocytes. It is a potent chemoattractant for monocytes and induces interleukin 1 mRNA expression while inhibiting generation of reactive oxygen intermediates and monocyte killing. Evidence is accumulating that TGF-beta regulates immune function in vivo and that overproduction of TGF-beta may be associated with immunosuppression.
Cembrene, a Fourteen-Membered Ring Diterpene Hydrocarbon<sup>*,1,2</sup> (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCembrene, a Fourteen-Membered Ring Diterpene Hydrocarbon*,1,2William G. Dauben, William E. Thiessen, and Paul R. ResnickCite this: J. Org. Chem. 1965, 30, 6, 1693–1698Publication Date (Print):June 1, 1965Publication History Published online1 May 2002Published inissue 1 June 1965https://pubs.acs.org/doi/10.1021/jo01017a001https://doi.org/10.1021/jo01017a001research-articleACS PublicationsRequest reuse permissionsArticle Views230Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Totalsynthese eines Tetrasaccharid‐Antigens von <i>Bacillus anthracis</i> – Basis für einen Impfstoff gegen Anthrax (2005)

Kann man Anthrax mit seinen eigenen Waffen schlagen? Der Anthrax-Erreger, Bacillus anthracis, trägt spezifische Oligosaccharide auf seiner Oberfläche, die für die erste Interaktion mit dem Wirt wahrscheinlich von großer Bedeutung sind. Ausgehend von diesen spezifischen Oligosacchariden können Impfstoffe entwickelt werden, die eine Immunantwort hervorrufen. Die Synthese eines solchen Impfstoff-Kandidaten wird vorgestellt (siehe Bild; Pent=4-Pentenyl). Hintergrundinformationen zu diesem Beitrag sind im WWW unter http://www.wiley-vch.de/contents/jc_2001/2005/z502615_s.pdf zu finden oder können beim Autor angefordert werden. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
An Internet of Energy Things Based on Wireless LPWAN (2017)

Under intense environmental pressure, the global energy sector is promoting the integration of renewable energy into interconnected energy systems. The demand-side management (DSM) of energy systems has drawn considerable industrial and academic attention in attempts to form new flexibilities to respond to variations in renewable energy inputs to the system. However, many DSM concepts are still in the experimental demonstration phase. One of the obstacles to DSM usage is that the current information infrastructure was mainly designed for centralized systems, and does not meet DSM requirements. To overcome this barrier, this paper proposes a novel information infrastructure named the Internet of Energy Things (IoET) in order to make DSM practicable by basing it on the latest wireless communication technology: the low-power wide-area network (LPWAN). The primary advantage of LPWAN over general packet radio service (GPRS) and area Internet of Things (IoT) is its wide-area coverage, which comes with minimum power consumption and maintenance costs. Against this background, this paper briefly reviews the representative LPWAN technologies of narrow-band Internet of Things (NB-IoT) and Long Range (LoRa) technology, and compares them with GPRS and area IoT technology. Next, a wireless-to-cloud architecture is proposed for the IoET, based on the main technical features of LPWAN. Finally, this paper looks forward to the potential of IoET in various DSM application scenarios.
Botulinum ADP‐ribosyltransferase C3 (1988)

A novel ADP-ribosyltransferase C3 was purified to homogeneity from filtrates of certain strains of Clostridium botulinum type C by ammonium sulfate precipitation, gel filtration, ion-exchange chromatography and heat treatment. The molecular mass of botulinum ADP-ribosyltransferase C3 was found to be 25 kDa. In the presence of [32P]NAD but not with [carbonyl-14C]NAD, C3 labelled 21-24-kDa protein(s) in membranes of human platelets and other tissues. The Km value of the ADP-ribosylation reaction for NAD was about 2 microM. Labelling of the 21-24-kDa protein(s) by C3 was largely reduced by addition of nicotinamide. Snake venom phosphodiesterase cleaved the ADP-ribose attached to the 21-24-kDa protein(s) by C3 and released 5'AMP. C3 catalyzed hydrolysis of [carbonyl-14C]NAD and released [carbonyl-14C]nicotinamide. ADP-ribosylation of 21-24-kDa platelet membrane protein(s) was biphasically regulated by Mg2+, Mn2+ and Ca2+. In the absence of free divalent cations GTP, GTP[gamma S] and GDP but not GDP[beta S], GMP, ATP or ATP[gamma S] increased labelling by C3. In the presence of Mg2+, GTP[gamma S] was inhibitory. Guanine nucleotides prevented heat inactivation of the substrate protein(s) with the rank order GTP[gamma S] = GTP = GDP greater than GDP[beta S] greater than GMP much greater than ATP = GMP = ATP[gamma S]. The data support the view that the novel ADP-ribosyltransferase C3 modifies eukaryotic 21-24-kDa GTP-binding protein(s).
Continuous Solid‐State Polycondensation of Polyesters (2004)

This chapter contains sections titled: Introduction The Chemical Reactions of PET in the Solid State Crystallization of PET Continuous Solid-state Polycondensation Processing PET Recycling References
<sup>31</sup>P NMR spectroscopic study of bioenergetic changes in radiation‐induced fibrosarcoma‐1 after radiation therapy (1989)

The effects of localized gamma-irradiation on the in vivo 31P NMR spectra of RIF-1 tumors grown subcutaneously in C3H/HeN mice have been examined before and during the week after treatment. Increases in the ratio of phosphocreatine (PCr) to inorganic phosphate (Pi) and in tumor pH, and decreases in the ratio of Pi to the beta phosphorus resonance of the nucleotide triphosphates (beta NTP) were observed in irradiated tumors. The time course of changes in the 31P spectrum following treatment was the opposite of the pattern during untreated growth, and the magnitude and duration of the changes increased with increasing radiation dose, decreasing clonogenic cell survival and increasing growth delay. To examine the possibility that nontherapeutic systemic effects of the tumor irradiation were responsible for the changes observed, a number of animals bearing two tumors were examined. One tumor on each mouse was selectively irradiated. Changes in tumor volume, Pi/beta NTP, PCr/Pi, the ratio of phosphomonoesters to beta NTP, and tumor pH were all significantly different in the treated compared to the untreated tumor on each animal, indicating that these changes in 31P NMR spectra were a response to radiation therapy and not a systemic response to radiation toxicity.
The Baptism of Edwin, King of Northumbria: A New Analysis of the British Tradition (2000)

(2000). The Baptism of Edwin, King of Northumbria: A New Analysis of the British Tradition. Northern History: Vol. 36, No. 1, pp. 5-15.
Second-Generation Low-Emission Combustors for ABB Gas Turbines: Burner Development and Tests at Atmospheric Pressure (1992)

Based on fundamental research concerning swirling flows, including the vortex breakdown phenomenon, as well as on stability considerations of premixed flames, a second generation of low-emission burners has been developed. The lean premixing technique provides NOx emissions below 25 ppmv for natural gas. For liquid fuels the oxides of nitrogen are limited to 42 ppmv (oil No. 2). The novel burner technology will be applied to the well-known ABB silo combustor. As a first step the Conical Premix Burner will be used to retrofit the ABB type 11N. For the ABB gas turbine type 8 the design of a novel fully annular combustor is in progress. Most of the conceptual work concerning burner aerodynamics and burner-burner interaction has been carried out on scaled-down burner and combustor models. For a second step a sector of the combustor in 1:1 scale has been tested at atmospheric pressure. Additional high-pressure tests provide information about the combustor performance at engine conditions. The present paper summarizes the results of the first two steps beginning with the early ideas in the conceptual phase up to the 1:1 tests, which prove the low-NOx capability for both gaseous and liquid fuels under atmospheric pressure conditions.
The Role of Equatorial and Axial Ligands in Promoting the Activity of Non‐Heme Oxidoiron(IV) Catalysts in Alkane Hydroxylation (2007)

Abstract The key electronic structural feature of the FeO 2+ moiety, which determines its activity as an alkane hydroxylation catalyst, is the presence of low‐lying acceptor orbitals, namely the 3σ* 3d –2p z antibonding orbital. Both the energetic position of this orbital and the spin state of the system (which in turn also affects the 3σ* energy) depend on the surrounding ligands. We present results of density functional theory (DFT) calculations performed on a series of gas‐phase complexes of composition [FeO(H 2 O) n (L) 5– n ] 2+ ( n = 4, 1, 0) derived from the recently characterised aqueous [FeO(H 2 O) 5 ] 2+ by substitution of ligand water molecules with L = NH 3 , CH 3 CN, H 2 S and BF 3 . The calculations reveal that the high‐spin (quintet) state is favoured by the weaker σ‐donating equatorial ligands, which is consistent with the literature. The high‐spin configuration is more reactive because of significant exchange stabilisation of the crucial 3σ*↑ orbital. Once the quintet state is formed by a judicious choice of equatorial ligands, the reactivity can be fine‐tuned by modulating the energy of the 3σ* orbital by varying the nature of the axial ligand. A linear relation between the σ‐donor properties of the axial ligand (estimated from the magnitude of the orbital interaction between the σ lone pair and the 3σ* orbital) and the activation barrier for the abstraction reaction is observed, and is related to a “push effect” of the σ donors that destabilises the 3σ* orbital. We propose that species with enhanced activation properties for hydrogen abstraction relative to [FeO(H 2 O) 5 ] 2+ might be obtainable by either replacing the axial ligand with a σ donor weaker than H 2 O or by preventing ligands from coordinating to iron in an axial position. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
IMS signalling for multiparty services based on network level multicast (2007)

The standardization process of the UMTS technology has led to the development of the IP Multimedia Subsystem (IMS). IMS provides a framework that supports the negotiation of the next generation multimedia services with QoS requirements that are envisioned for 3G networks. But even though many of these services involve the participation of multiple users in a multiparty arrangement, the delivery technology at network level is still unicast based. This approach is not optimum, in terms of transmission efficiency. In this paper, a new approach is presented proposing to use a network level multicast delivery technology for the multiparty services that are signalled through IMS. The main advantages and drawbacks related with this new approach are analyzed in the article. Finally, as a starting point in the development of the presented solution, a new SIP signalling dialogue is proposed allowing the negotiation of a generic multiparty service, and supporting at the same time the configuration of the corresponding network level multicast delivery service with QoS requirements that will be used in the user plane.
Biosynthesis of 5‐deoxyflavanones in microorganisms (2007)

Flavanones are the common precursors of plant polyphenolic compounds collectively known as flavonoids. Leguminous plants have evolved a distinct class of flavanone molecules, known as 5-deoxyflavanones that play important roles in their symbiotic interactions. A four-step metabolic circuit was constructed in Escherichia coli with plant genes from heterologous origins: 4-coumarate:coenzyme A ligase from Petroselinum crispum, chalcone synthases (CHS) from Medicago sativa and Petunia x hybrida and chalcone reductase and chalcone isomerase from M. sativa. Evaluation of the different recombinant strains in shake flask experiments demonstrated that P. hybrida rather than M. sativa CHS resulted in the highest liquiritigenin production levels in glucose minimal medium, starting from precursor p-coumaric acid. Expression of the same recombinant pathway in Saccharomyces cerevisiae resulted in the accumulation of both 5-hydroxyflavanone and 5-deoxyflavanone, with the yields of the later lower than that achieved in E. coli. Other phenylpropanoid acid precursors, such as cinnamic acid and caffeic acid could also be metabolized through the recombinant pathway, yielding corresponding 5-deoxyflavanone compounds. The construction of such recombinant strains for 5-deoxyflavanone biosynthesis offers an alternative way to biochemically characterize flavonoid biosynthetic enzymes and promising production platforms for the biosynthesis of such high-value natural products.
Unsymmetrical Cyanostilbenes (1942)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUnsymmetrical CyanostilbenesJoseph B. Niederl and Albert ZieringCite this: J. Am. Chem. Soc. 1942, 64, 4, 885–886Publication Date (Print):April 1, 1942Publication History Published online1 May 2002Published inissue 1 April 1942https://pubs.acs.org/doi/10.1021/ja01256a042https://doi.org/10.1021/ja01256a042research-articleACS PublicationsRequest reuse permissionsArticle Views205Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Healthy Older Observers Show Equivalent Perceptual-Cognitive Training Benefits to Young Adults for Multiple Object Tracking (2013)

The capacity to process complex dynamic scenes is of critical importance in real life. For instance, traveling through a crowd while avoiding collisions and maintaining orientation and good motor control requires fluent and continuous perceptual-cognitive processing. It is well documented that effects of healthy aging can influence perceptual-cognitive processes (Faubert, 2002) and that the efficiency of such processes can improve with training even for older adults (Richards et al., 2006). Here we assess the capacity of older participants to improve their tracking speed thresholds in a dynamic, virtual reality environment. Results show that this capacity is significantly affected by healthy aging but that perceptual-cognitive training can significantly reduce age-related effects in older individuals, who show an identical learning function to younger healthy adults. Data support the notion that learning in healthy older persons is maintained for processing complex dynamic scenes.
The Alternative Sigma Factor SigH Regulates Major Components of Oxidative and Heat Stress Responses in<i>Mycobacterium tuberculosis</i> (2001)

Mycobacterium tuberculosis is a specialized intracellular pathogen that must regulate gene expression to overcome stresses produced by host defenses during infection. SigH is an alternative sigma factor that we have previously shown plays a role in the response to stress of the saprophyte Mycobacterium smegmatis. In this work we investigated the role of sigH in the M. tuberculosis response to heat and oxidative stress. We determined that a M. tuberculosis sigH mutant is more susceptible to oxidative stresses and that the inducible expression of the thioredoxin reductase/thioredoxin genes trxB2/trxC and a gene of unknown function, Rv2466c, is regulated by sigH via expression from promoters directly recognized by SigH. We also determined that the sigH mutant is more susceptible to heat stress and that inducible expression of the heat shock genes dnaK and clpB is positively regulated by sigH. The induction of these heat shock gene promoters but not of other SigH-dependent promoters was markedly greater in response to heat versus oxidative stress, consistent with their additional regulation by a heat-labile repressor. To further understand the role of sigH in the M. tuberculosis stress response, we investigated the regulation of the stress-responsive sigma factor genes sigE and sigB. We determined that inducible expression of sigE is regulated by sigH and that basal and inducible expression of sigB is dependent on sigE and sigH. These data indicate that sigH plays a central role in a network that regulates heat and oxidative-stress responses that are likely to be important in M. tuberculosis pathogenesis.
Isolation of Bioactive Phytoconstituent from<i>Alpinia galanga</i>L. with Anti-Hyperlipidemic Activity (2013)

The present study was undertaken to explore the antihyperlipidemic effect of ethanolic extract of rhizomes of Alpinia galanga L. and its chloroform fraction in Triton-induced hyperlipidemic rats. Bioactivity guided fractionation was followed by chromatographic studies. Flash chromatography was done for the most active fraction resulting in the isolation of 5-(hydroxymethyl) furfural. Animals were administered with i.p. injection of Triton WR 1339 at dose of 400 mg/kg body weight. After 24 hr of Triton administration, the ethanolic extract and its fraction were administered orally at doses of 200 and 400 mg/kg body weight in rats. The treatment was continued for 5 days with a view to see the effect on lipid profile. Serum samples were subjected to biochemical analysis. The study dose dependently inhibited the total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL) level, and significantly increased high-density lipoprotein (HDL) level. Phytochemical screening revealed the presence of tannins, coumarins, flavanoids, sterols, and glycosides. Phytochemical investigation of the chloroform fraction of A. galanga L. resulted in the isolation of 5-(hydroxymethyl) furfural. UV λmax was found to be 276 nm for the isolated component. Acute treatment caused a stimulatory effect on the HDL level and inhibition in TC and TG elevation induced by triton.
Visualization of Communication Patterns in Collaborative Innovation Networks - Analysis of Some W3C Working Groups (2003)

Collaborative Innovation Networks (COINs) are groups of self-motivated individuals from various parts of an organization or from multiple organizations, empowered by the Internet, who work together on a new idea, driven by a common vision. In this paper we report first results of a project that examines innovation networks by analyzing the e-mail archives of some W3C (WWW consortium) working groups. These groups exhibit ideal characteristics for our purpose, as they form truly global networks working together over the Internet to develop next generation technologies. We first describe the software tools we developed to visualize the temporal communication flow, which represent communication patterns as directed acyclic graphs, We then show initial results, which revealed significant variations between the communication patterns and network structures of the different groups., We were also able to identify distinctive communication patterns among group leaders, both those who were officially appointed and other who were assuming unofficial coordinating roles.