gebhart/patrepeval huggingface dataset:
High-resolution in vivo imaging of mouse brain through the intact skull (2015)

Multiphoton microscopy is the current method of choice for in vivo deep-tissue imaging. The long laser wavelength suffers less scattering, and the 3D-confined excitation permits the use of scattered signal light. However, the imaging depth is still limited because of the complex refractive index distribution of biological tissue, which scrambles the incident light and destroys the optical focus needed for high resolution imaging. Here, we demonstrate a wavefront-shaping scheme that allows clear imaging through extremely turbid biological tissue, such as the skull, over an extended corrected field of view (FOV). The complex wavefront correction is obtained and directly conjugated to the turbid layer in a noninvasive manner. Using this technique, we demonstrate in vivo submicron-resolution imaging of neural dendrites and microglia dynamics through the intact skulls of adult mice. This is the first observation, to our knowledge, of dynamic morphological changes of microglia through the intact skull, allowing truly noninvasive studies of microglial immune activities free from external perturbations.
Vascular pharmacology of a novel cannabinoid‐like compound, 3‐(5‐dimethylcarbamoyl‐pent‐1‐enyl)‐<i>N</i>‐(2‐hydroxy‐1‐methyl‐ethyl)benzamide (VSN16) in the rat (2007)

VSN16 is an agonist at a novel cannabinoid receptor of the vasculature. It acts on the endothelium to release nitric oxide and activate KCa and TRPV1. As it is water-soluble it might be useful in bringing about peripheral cannabinoid-like effects without accompanying central or severe cardiovascular responses.
Circulating levels of miR-146a and IL-17 are significantly correlated with the clinical activity of Graves’ ophthalmopathy (2014)

Graves' ophthalmopathy (GO) is a common autoimmune disease that is difficult to deal with due to limited clinical evaluation methods. Recently miR-146a and Interleukin-17 (IL-17) have been found to be involved in autoimmune disorders and correlated with disease activity. However, it is unclear whether they are involved in Graves' ophthalmopathy (GO). The aim of this study is to investigate the correlation of circulating levels of miR-146a and IL-17 with clinical activity in GO patients. Fifty-seven study subjects were enrolled in four groups according to the corresponding criteria: active-GO, inactive-GO, Graves disease (GD) without ophthalmopathy, and healthy control group. The circulating levels of miR-146a and IL-17 were determined by qRT-PCR and ELISA, respectively. Serum IL-17 levels of GD, inactive-GO, and active-GO groups were all significantly higher than that of control (all P < 0.001). Active-GO group had significantly higher IL-17 level than inactive-GO and GD groups (P = 0.024 and P = 0.001, respectively). Active-GO and inactive-GO group had significantly lower miR-146a expressions than control (P < 0.05). Active-GO group had significantly lower miR-146a than inactive-GO group (P < 0.05). Serum levels of IL-17 and miR-146a were both significantly correlated with clinical activity score (CAS) in GO patients (P < 0.001, P < 0.001, respectively). There was a significant negative correlation of circulating miR-146a expression with serum IL-17 levels (P < 0.01). These findings indicated that circulating levels of miR-146a and IL-17 may be potential biomarkers of active GO, and may play a key role in the progression of GO.
Autologous bone marrow transplantation for first remission acute myeloblastic leukemia in patients older than 50 years: a retrospective analysis of the European Bone Marrow Transplant Group (1995)

High-dose chemotherapy, with or without radiotherapy, followed by autologous stem-cell rescue is used increasingly for the intensification of first remission in acute myeloblastic leukemia (AML). However, these treatments have been limited to young patients due to the increased risks of regimen-related toxicities and mortality with age. Several investigators have recently published the upper age limit for autologous bone marrow transplant (ABMT) in AML because of encouraging results. The results of ABMT for AML were studied in 111 patients > or = 50 years of age intensified in first remission. Median age at transplant was 53 years (range, 50 to 63 years). Fifty patients were conditioned with total body irradiation and 61 with polychemotherapy: 23 with busulfancyclophosphamide, 11 with the University College Hospital (UCH; London, UK) regimen, 6 with BAVC, and 21 with various other treatments. Marrow was purged in only 11 cases. Results were compared with 786 ABMTs performed for AML in patients between 16 and 49 years of age (median, 35 years). For AML in first remission, the probability of leukemia-free survival (LFS) at 4 years was 34% +/- 5% for patients aged 50 years or more and 43% +/- 2% for patients less than 50 years of age (P = .004), with a survival probability of 35% +/- 6% and 48% +/- 2%, respectively (P = .004). The probability of relapse was not significantly different between the two groups (52% +/- 7% v 50% +/- 2%), but transplant-related mortality was significantly higher in the older age group (28% +/- 5% v 14% +/- 2%; P < .0001) and mainly due to infectious complications. In a multivariate analysis, age less than 50 years was a favorable risk factor for LFS, treatment-related mortality (TRM), and survival but not for relapse incidence. These data suggest that ABMT should be considered in older AML patients.
An effective technique for extended proximal contacts in composite resin restorations. (1996)

Obtaining a firm anatomic contact has been a difficult criteria in placing Class II posterior composite restorations. Various techniques have been developed and a number of products marketed to accomplish this task, but none have combined the necessary qualifications of ease, simplicity, and effectiveness for consistent and predictable results. To address this challenge, two innovative devices--a precontoured (biplanar concave) sectional matrix band and a light--focusing tip-have been developed. Using a clinical case presentation, this article describes the application of these devices to obtain high quality proximal contacts, even in widely separated teeth. The learning objective of this article is to familiarize clinicians with this procedure. The technique is simple and universally applicable with a variety of posterior composite materials and placement methods.
Chemically etched-mirror GaInAsP/InP lasers - Review (1982)

Detailed results on stripe Ga <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</inf> In <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1-x</inf> As <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">y</inf> P <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1-y</inf> /InP lasers ( <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\lambda = 1.3 \mu</tex> m) with chemically etched-mirrors are reviewed. These devices are fabricated from GaInAsP/InP wafers grown by liquid phase epitaxy. A simple stripe laser structure with one etched mirror and one cleaved mirror is proposed. Monolithic passivation has been achieved using a Si <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> film and metal coatings on the etched facets. These processes not only increase the reflectivity of the etched mirrors, resulting in threshold currents even lower than uncoated cleaved devices, but also ease the problem of bonding of the chips on heat sinks. CW operation at room temperature has been achieved. Threshold currents of devices with 10 μm stripe electrodes were about 180-200 mA. Short cavity lasers and integrated monitoring detectors have also been demonstrated.
Synthesis and Study of a Lipophilic <i>α</i>-Keto Amide Inhibitor of Pancreatic Lipase (2000)

[reaction: see text] A lipophilic alpha-keto amide, inhibitor of pancreatic lipase, was synthesized using a lipidic 2-amino alcohol as backbone. The chiral key intermediate 2-(tert-butyloxycarbonylamino)-D-undecen-5-ol was synthesized starting from D-glutamic acid. The inhibitor formed a stable monomolecular film at the air/water interface as shown by a force/area curve. Inhibition studies using the monomolecular film technique with mixed films of 1,2-dicaprin containing variable proportions of the inhibitor showed a 50% decrease in lipase activity at a 0.14 molar fraction.
Zinc-secreting Paneth Cells Studied by ZP Fluorescence (2005)

We have used a new family of zinc-specific-responsive fluorescent dyes (ZPs) to study the sequestration and secretion of zinc from Paneth cells, which are located in the bases of the crypts of Lieberkühn within the rat small intestine. Vivid ZP fluorescence zinc staining of Paneth cell secretory granules is seen in both cryostat sections and isolated crypts, providing firm evidence for a pool of labile (rapidly exchangeable) zinc within these cells. We further demonstrate that this ionic zinc pool is secreted under physiological conditions. In vivo stimulation of the small intestine by IP injection of the secretagogue pilocarpine results in discrete zinc staining within the lumens of subsequently isolated crypts, concomitant with a decrease in the zinc staining of Paneth cell granules located within the same crypts. In contrast, the secretion of zinc into the lumens of isolated crypts stimulated in vitro with either carbachol or LPS (lipopolysaccharide) is not observed. However, a distinct change in Paneth cell morphology, suggesting attempted secretion, is seen in response to the direct application of cholinergics but not LPS. These findings suggest that zinc is coreleased with other Paneth cell anti-microbials, and that the intact intestine is necessary for secretion into the crypt lumen.
Corannulenylferrocenes: towards a 1D, non-covalent metal–organic nanowire (2012)

A class of novel corannulene-derived ferrocene donor-acceptor systems has been synthesized by straight-forward Negishi-type coupling of iodocorannulene. Their solid state structures have been studied crystallographically and found to exhibit unique inter- and intramolecular slipped stacking interactions.
Heart rate spectrum analysis for sleep quality detection (2017)

To evaluate the quality of sleep, it is important to determine how much time was spent in each sleep stage during the night. The gold standard in this domain is an overnight polysomnography (PSG). But the recording of the necessary electrophysiological signals is extensive and complex and the environment of the sleep laboratory, which is unfamiliar to the patient, might lead to distorted results. In this paper, a sleep stage detection algorithm is proposed that uses only the heart rate signal, derived from electrocardiogram (ECG), as a discriminator. This would make it possible for sleep analysis to be performed at home, saving a lot of effort and money. From the heart rate, using the fast Fourier transformation (FFT), three parameters were calculated in order to distinguish between the different sleep stages. ECG data along with a hypnogram scored by professionals was used from Physionet database, making it easy to compare the results. With an agreement rate of 41.3%, this approach is a good foundation for future research.
TSPS No. 1: Peripheral Circuits (1970)

The TSPS No. 1 peripheral circuits permit the data processing ability of the Stored Program Control No. 1A to be applied to TSPS functions. These circuits include scanners, signal distributors, networks, trunk and service circuits, operator positions and the position subsystem. A description of each circuit is given, followed by a description of the features provided by the associated maintenance program.
Dibenzyl Sulfide Metabolism by White Rot Fungi (2003)

Microbial metabolism of organosulfur compounds is of interest in the petroleum industry for in-field viscosity reduction and desulfurization. Here, dibenzyl sulfide (DBS) metabolism in white rot fungi was studied. Trametes trogii UAMH 8156, Trametes hirsuta UAMH 8165, Phanerochaete chrysosporium ATCC 24725, Trametes versicolor IFO 30340 (formerly Coriolus sp.), and Tyromyces palustris IFO 30339 all oxidized DBS to dibenzyl sulfoxide prior to oxidation to dibenzyl sulfone. The cytochrome P-450 inhibitor 1-aminobenzotriazole eliminated dibenzyl sulfoxide oxidation. Laccase activity (0.15 U/ml) was detected in the Trametes cultures, and concentrated culture supernatant and pure laccase catalyzed DBS oxidation to dibenzyl sulfoxide more efficiently in the presence of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) than in its absence. These data suggest that the first oxidation step is catalyzed by extracellular enzymes but that subsequent metabolism is cytochrome P-450 mediated.
Cleaning robot control (2003)

A small and lightweight cleaning robot powered from the AC power supply is produced for testing purposes. The robot provides a cable-length control function which prevents tangling of the cables during traveling, an ultrasonic sensor function which detects obstacles and dodges them, and a distance measuring function which makes it possible to run parallel to the wall. In a simple room with few obstacles, the robot can travel even if it does not incorporate information but in areas with complicated placement of obstacles, it is necessary to teach the robot the obstacle positions and the room size in advance.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Body-centric design space for multi-surface interaction (2013)

We introduce BodyScape, a body-centric design space that allows us to describe, classify and systematically compare multi-surface interaction techniques, both individually and in combination. BodyScape reflects the relationship between users and their environment, specifically how different body parts enhance or restrict movement within particular interaction techniques and can be used to analyze existing techniques or suggest new ones. We illustrate the use of BodyScape by comparing two free-hand techniques, on-body touch and mid-air pointing, first separately, then combined. We found that touching the torso is faster than touching the lower legs, since it affects the user's balance; and touching targets on the dominant arm is slower than targets on the torso because the user must compensate for the applied force.
Sensitive Method for the Kinetic Measurement of Trace Species in Liquids Using Cavity Enhanced Absorption Spectroscopy with Broad Bandwidth Supercontinuum Radiation (2010)

A novel spectrometer for the rapid and sensitive detection of liquid phase analytes at trace concentrations is presented. Broad bandwidth supercontinuum radiation was coupled into a linear optical cavity incorporating an intracavity liquid-sample cuvette. Cavity enhanced absorption spectra of trace species covering more than 300 nm were acquired on time scales of milliseconds. Single shot acquisition times of 10-50 ms are demonstrated here. The effective absorption path length exceeds 2 m in sample volumes measuring 2.7 mL. A key feature of the instrument is that it can be calibrated using cavity ring-down spectroscopy without the requirement of changing the optical alignment. The sensitivity of the instrument is exemplified by measurements of trace concentrations of dye molecules and nickel sulfate dissolved in water. A minimum detectable absorption coefficient of 9.1 x 10(-7) cm(-1) Hz(-1/2) at 550 nm was obtained. The capability to capture broad bandwidth absorption spectra on short time scales permits kinetic studies of liquid phase reactions. We demonstrate this by recording the oscillatory behavior of a Belousov-Zhabotinsky reaction.
Development of a new rhodamine-based FRET platform and its application as a Cu2+ probe (2014)

We have constructed a new rhodamine-based FRET platform, which was then used to develop a ratiometric fluorescent Cu(2+) probe. The novel Cu(2+) probe exhibits several favorable features including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, and high selectivity. Importantly, it is suitable for fluorescence imaging in living cells.
Bandwidth Packing: A Tabu Search Approach (1993)

The bandwidth packing (BWP) problem is a combinatorially difficult problem arising in the area of telecommunications. The problem consists of assigning calls to paths in a capacitated graph, such that capacities are not violated and the total profit is maximized. In this paper we discuss the development of a tabu search (TS) method for the BWP problem. The method makes use of an efficient implementation of the k-shortest path algorithm, that allows the identification of a controlled set of feasible paths for each call. A tabu search is then performed to find the best path assignment for each call. The TS method developed here incorporates a number of features that have proved useful for obtaining optimal and near optimal solutions to difficult combinatorial problems. We establish the effectiveness of our approach by comparing its performance in speed and solution quality to other specialized heuristics and to a standard optimization package applied to a 0-1 integer programming formulation of the problem.
Effect of alkaline leaching conditions on coal mineral matter (1985)

The fate of the mineral matter was observed when coal was leached with hot alkaline solutions. The principal solid conversion product of pyrite was hematite but under some conditions small amounts of magnetite and pyrrhotite were formed. The clay minerals were converted to various sodium hydroaluminosilicates, and the quartz was extracted. However, under some conditions amorphous silica was precipitated when the leachant was cooled. 7 refs., 3 tabs.
Adenosine 5-Triphosphate Induced Dilation of Human Coronary Microvessels In Vivo. (1999)

These results suggest that ATP induces maximal dilation of coronary microvessels, most likely through an endothelium-independent mechanism. The degradation of ATP to adenosine 5'-monophosphate (AMP) and adenosine, as well as the direct action of ATP on A2-adenosine receptors may be responsible for the dilation. We conclude that coronary flow reserve can be determined safely with intracoronary ATP administration.
Acyclic diene metathesis polymerization: The synthesis of unsaturated polyketones (1993)

Abstract The first acyclic diene metathesis (ADMET) polymerization of unsaturated ketone containing monomers using the molybdenum catalyst, Mo(CHCMe 2 Ph)(N‐2,6‐C 6 H 3 ‐ i ‐Pr 2 )[OCCH 3 (CF 3 ) 2 ] 2 is reported. 6,6,8,8‐Tetramethyl‐1,12‐tridecadiene‐7‐one undergoes homopolymerization; copoly‐merizations are carried out with 1,9‐decadiene and 2,12‐dimethyl‐2,12‐dipentenylcyclododecan‐1‐one, 2,12‐diallylcyclododecan‐1‐one, and trans ‐2,12‐diallyl‐2,12‐dimethylcyclododecan‐1‐one These polymerizations are initiated under bulk conditions and are continued in solution. No evidence of Wittig chemistry is observed between the carbonyl functional group and the catalyst when a high degree of steric hindrance exists around the carbonyl moiety. Polymer structures were characterized by IR, 1 H NMR, and 13 C NMR spectroscopy. Molecular weights were determined by endgroup analysis and gel permeation chromatography.
Choroid plexus epithelial expression of <i>MDR</i> <i>1</i> P glycoprotein and multidrug resistance-associated protein contribute to the blood–cerebrospinal-fluid drug-permeability barrier (1999)

The blood-brain barrier and a blood-cerebrospinal-fluid (CSF) barrier function together to isolate the brain from circulating drugs, toxins, and xenobiotics. The blood-CSF drug-permeability barrier is localized to the epithelium of the choroid plexus (CP). However, the molecular mechanisms regulating drug permeability across the CP epithelium are defined poorly. Herein, we describe a drug-permeability barrier in human and rodent CP mediated by epithelial-specific expression of the MDR1 (multidrug resistance) P glycoprotein (Pgp) and the multidrug resistance-associated protein (MRP). Noninvasive single-photon-emission computed tomography with 99mTc-sestamibi, a membrane-permeant radiopharmaceutical whose transport is mediated by both Pgp and MRP, shows a large blood-to-CSF concentration gradient across intact CP epithelium in humans in vivo. In rats, pharmacokinetic analysis with 99mTc-sestamibi determined the concentration gradient to be greater than 100-fold. In membrane fractions of isolated native CP from rat, mouse, and human, the 170-kDa Pgp and 190-kDa MRP are identified readily. Furthermore, the murine proteins are absent in CP isolated from their respective mdr1a/1b(-/-) and mrp(-/-) gene knockout littermates. As determined by immunohistochemical and drug-transport analysis of native CP and polarized epithelial cell cultures derived from neonatal rat CP, Pgp localizes subapically, conferring an apical-to-basal transepithelial permeation barrier to radiolabeled drugs. Conversely, MRP localizes basolaterally, conferring an opposing basal-to-apical drug-permeation barrier. Together, these transporters may coordinate secretion and reabsorption of natural product substrates and therapeutic drugs, including chemotherapeutic agents, antipsychotics, and HIV protease inhibitors, into and out of the central nervous system.
Editorial (2015)

Campos Salles agradecem aos autores que se dedicaram a desenvolver ideias e tecer
Macrophages have cell surface IL-10 that regulates macrophage bactericidal activity (1996)

IL-10, which is secreted by multiple cell types, has regulatory effects on macrophages, including decreasing their ability to kill some microorganisms. The experiments presented here test the hypothesis that endogenous IL-10 inhibits the ability of macrophages to kill the facultative intracellular bacterium, Listeria monocytogenes. We show that the nonbactericidal macrophage hybrid, H36.12j (12j), can kill Listeria after incubation for 3 days with anti-IL-10 mAb. IL-10 was not detected in 12j macrophage supernatants by ELISA. However, flow cytometric analysis revealed high levels of IL-10 on the 12j cell surface. This indicates that macrophages that fail to secrete IL-10 may express IL-10 on the cell surface, and this IL-10 appears to suppress listericidal activity. Surface IL-10 is not unique to the 12j macrophage hybrid and may correlate with the absence of bactericidal activity in other macrophages. For instance, nonlistericidal resident and thioglycolate-elicited peritoneal exudate cells have 24 to 72% IL-10-positive macrophages. In contrast listericidal proteose peptone-elicited peritoneal exudate cells contained < 5% IL-10-positive macrophages. Whether this IL-10 is an integral membrane protein or is bound to IL-10 receptors is not yet known. However, the IL-10 does not elute with acid as other passively bound molecules do, nor does exogenous IL-10 bind to macrophages. In either case, since anti-IL-10 induces nonbactericidal macrophages to become bactericidal, the surface IL-10 is biologically active, and it appears to regulate macrophage bactericidal activity.