gebhart/patrepeval huggingface dataset:
The Production of Catechols from Benzene and Toluene by<i>Pseudomonas Putida</i>in Glucose Fed-Batch Culture (1992)

Catechol and 3-methylcatechol were produced from benzene and toluene respectively using different mutants of Pseudomonas putida. P. putida 2313 lacked the extradiol cleavage enzyme, catechol 2,3-oxygenase, allowing overproduction of 3-methylcatechol from toluene to a level of 11.5 mM (1.27 g·1-1) in glucose fed-batch culture. P. putida 6(12), a mutant of P. putida 2313, lacked both catechol-oxygenase and catechol 1,2-oxygenase, and accumulated catechol from benzene to a level of 27.5mM(3g·1-1). In both biotransformations product formation ceased within 10 hours of feeding the aromatic substrate, and this was due to product inhibition by the catechols. The primary site of catechol toxicity was inhibition of the aromatic dioxygenase. Neither cis-toluene dihydrodiol cis-1,2-dihydroxy-3-methylcyclohexa-3,5-diene), nor cis-benzene dihydrodiol (cis-l,2-dihydroxy-3-methylcyclohexa-3,5-diene) dehydrogenase was significantly inhibited by catechol overproduction whereas both ring activating dioxygenases were inhibited within 4-6 hours of the maximum product concentration being attained. 3-Methylcatechol overproduction from toluene was also studied using a continuous product removal system. Granular activated charcoal removed 3-methylcatechol efficiently and was easily regenerated by washing with ethyl acetate. Using P. putida 2313, it was shown that the final product concentration increased approximately fourfold. Additional products were formed and the significance of these are discussed. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Model of Pulsed Electrical Discharge Machining (EDM) using RL Circuit (2014)

This article presents a model of pulsed Electrical Discharge Machining (EDM) using RL circuit. There are several mathematical models have been successfully developed based on the initial, ignition and discharge phase of current and voltage gap. According to these models, the circuit schematic of transistor pulse power generator has been designed using electrical model in Matlab Simulink software to identify the profile of voltage and current during machining process. Then, the simulation results are compared with the experimental results.
Relationships among Vitamin D Levels, Parathyroid Hormone, and Calcium Absorption in Young Adolescents (2005)

These data suggest that in adolescents, especially in the presence of vitamin D insufficiency, PTH secretion increases to adapt to higher rates of bone formation associated with growth. This results in higher serum 1,25(OH)2D concentrations and increased calcium absorption results. Vitamin D status, as reflected by the serum 25-OHD level, is not closely related to calcium absorption. Whether adaptation to low serum 25-OHD is adequate under physiologically stressful situations, including those leading to very low serum 25-OHD levels, is unknown.
High-speed 2D and 3D fluorescence microscopy of cardiac myocytes (2011)

Oblique plane microscopy (OPM) is a light sheet microscopy technique that uses a single high numerical aperture microscope objective to both illuminate a tilted plane within the specimen and to obtain an image of the tilted illuminated plane. In this paper, we present a new OPM configuration that enables both the illumination and detection focal planes to be swept simultaneously and remotely through the sample volume, enabling high speed volumetric imaging. We demonstrate the high speed imaging capabilities of the system by imaging calcium dynamics in cardiac myocytes in 2D at 926 frames per second and in 3D at 21 volumes per second. In the future, higher frame rate CCD cameras will enable volumetric imaging at much greater rates, leading to new capabilities to study dynamic events in cells at high speeds in two and three dimensions.
High-speed time interleaved ADCs (2016)

Software-defined multi-gigahertz receivers require high-speed ADCs at the frontend. Time interleaving has emerged as the most common method of achieving ultra-fast quantization at reasonably high resolution. However, this multi-path solution introduces systematic errors due to mismatches in signal paths, whereas in non-interleaved versions these were mixed to DC, where they appeared as a harmless offset. Mitigating all possible time-interleaved errors comes at a heavy cost in complexity, risk, and power. Knowing which errors are most important and which can be neglected in any given application is essential for picking an appropriate architecture and calibration scheme. Guidelines for reducing errors lead to potentially different architecture choices. When the goal is to use the fewest slices, an inerleaved pipelined ADC results, whereas when the overriding objective is to use the simplest slice possible, a large array of SAR slices is usually adopted. Both approaches have merit. This article addresses when and where to use each approach by discussing specification requirements and showing that different types of error sources should not be merged into one single metric like ENOB, but should be treated separately to determine their impact on overall system performance. An example of an eight-way interleaved pipelined ADC is presented, which illustrates these principles in the context of a real circuit.
Vibrational spectra nad force constants of the square-pyramidal anions SF5-, SeF5-, and TeF5- (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVibrational spectra nad force constants of the square-pyramidal anions SF5-, SeF5-, and TeF5-Karl O. Christe, E. C. Curtis, Carl J. Schack, and D. PilipovichCite this: Inorg. Chem. 1972, 11, 7, 1679–1682Publication Date (Print):July 1, 1972Publication History Published online1 May 2002Published inissue 1 July 1972https://doi.org/10.1021/ic50113a046Request reuse permissionsArticle Views283Altmetric-Citations82LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (496 KB) Get e-Alertsclose Get e-Alerts
Effect of Feeding Conjugated Linoleic Acid over Different Periods of Weight Gain on Swine Performance and Body Composition (1999)

Pigs fed conjugated linoleic acid (CLA) for the longest period had increased gain:feed ratios, decreased backfat thickness, and increased loin muscle area. Most of the performance responses to CLA, however, were obtained with shorter periods of feeding CLA.
Daptomycin Clearance during Modeled Continuous Renal Replacement Therapy (2006)

The extent of daptomycin's transmembrane clearance is dependent on hemodiafilter type, dialysate and ultrafiltration rates. CRRT with high ultrafiltrate or dialysate rates may result in substantial daptomycin clearances.
Design of a haptic device for teleoperation and virtual reality systems (2009)

Haptics technology has increased the precision and telepresence of the teleoperation and precision of the in-house robotic applications by force and surface information feedback. Force feedback is achieved through sending back the pressure and force information via a haptic device as the information is created or measured at the point of interest. In order to configure such a system, design, analysis and production processes of a haptic device, which is suitable for that specific application, becomes important. Today, haptic devices find use in assistive surgical robotics and most of the teleoperation systems. These devices are also extensively utilized in simulators to train medical and military personnel. The objective of this work is to design a haptic device with a new structure that has the potential to increase the precision of the robotic operation. Thus, literature is reviewed and possible robot manipulator designs are investigated to increase the precision in haptics applications. As a result of the investigations, conceptual designs are developed. Ultimately, final design is selected and produced after it is investigated in computer-aided-design (CAD) environment and its kinematic and structural analyses are carried out.
Pressure build-up characteristics in Austin Chalk wells (1983)

The vast amount of RNA-seq data deposited in Gene Expression Omnibus (GEO) and Sequence Read Archive (SRA) is still a grossly underutilized resource for biomedical research. To remove technical roadblocks for reusing these data, we have developed a web-application GREIN (GEO RNA-seq Experiments Interactive Navigator) which provides user-friendly interfaces to manipulate and analyze GEO RNA-seq data. GREIN is powered by the back-end computational pipeline for uniform processing of RNA-seq data and the large number (>6,500) of already processed datasets. The front-end user interfaces provide a wealth of user-analytics options including sub-setting and downloading processed data, interactive visualization, statistical power analyses, construction of differential gene expression signatures and their comprehensive functional characterization, and connectivity analysis with LINCS L1000 data. The combination of the massive amount of back-end data and front-end analytics options driven by user-friendly interfaces makes GREIN a unique open-source resource for re-using GEO RNA-seq data. GREIN is accessible at: https://shiny.ilincs.org/grein , the source code at: https://github.com/uc-bd2k/grein , and the Docker container at: https://hub.docker.com/r/ucbd2k/grein .
Ion Thruster Optical Performance Enhancement via Ion-emissive-surface Shaping (2005)

Abstract: Numerical results that demonstrate how ion emissive surfaces should be shaped to induce the formation of well-focused, high-current beamlets are presented. Results obtained when the sheath shape and position are established and then held constant as operating conditions are changed are compared to corresponding results obtained with a conventional plasma sheath that moves. These comparisons show the shaped surface: 1) yields beamlets that remain focused over wider beamlet current ranges, 2) enables operation without a crossover limit, and 3) enables operation at lower net-to-total accelerating voltage ratios and, therefore, at lower specific impulses. It is also shown that even greater beamlet currents can be extracted before the perveance limit is encountered if the propellant utilization efficiency approaches 100% and it is no longer necessary to have a small accel hole diameter to limit neutral propellant loss. Such a propellant utilization efficiency is expected for the Emissive Membrane Ion Thruster because its membrane is able to pass only ionized propellant. d = diameter (ds – screen, da – accel, dd- decel) E = electric field strength J = current (Jb – beamlet, Ji – impingement) r = radius (rs – screen, ra – accel) lg = grid spacing u = Bezier function independent variable V = voltage (Va – accel, Vd – decel, VN – net, VT – total) z = axial position I.
Delivery Agents for Oligonucleotides (2004)

This chapter provides a basic overview of most of the oligonucleotide delivery systems available for an in vitro use. Two major classes are described: systems that act through an endocytosis process (e.g., lipid-based vectors, nanoparticles, and polycations) and systems that by-pass this endocytosis process (e.g., peptides and pore-forming agents). Each technique is briefly described to allow a critical choice of the best delivery systems suitable for specific purposes in cultured cells.
Dose-dependent effects of bupivacaine on maternal hemodynamics in combined with spinal-epidural anesthesia for cesarean delivery. (2010)

Objective To study dose-dependent effects on maternal hemodynamics of bupivacaine in combined-spinal epidural anesthesia for cesarean section.Methods Ninety patients ready for caesarean section were recruited and randomly divided into three groups(group A,group B and group C with 30 cases for each group)and received combined with spinal-epidural anesthesia(CSEA).Patients were injected intrathecally with 0.5% bupivacaine(0.75%bupivacaine 2 mL+NS 1 mL)1.3 mL(group A),160 cm:1.2 mL,160～165 cm:1.3 mL,165～170 cm:1.4 mL,170 cm:1.5 mL(group B)and 1.0 mL+2% epidural lidocaine 3 mL(group C).Additional 2% lidocaine were added if necessary.If the systolic pressure was less than 90 mmHg or the diastolic pressure was 20% less than standard values,10 mg ephedrine would be used to correct it.Then all medical indexes were observed as follows:time from anesthesia to skin incision and operation time were recorded;meanwhile the blockade effectiveness,and the dosage of ephedrine requirements as well as patients heart rate,blood pressure,and blood oxygen saturation at each post-anesthetic time interval were recorded.Side effects such as nausea,vomiting,hypotension and chest distress were also observed.Results There was no significant difference in the time from anesthesia to skin incision and operation time among three groups.Blockage effect of group A(1.0±0.0)and group B(1.0±0.0)were superior to group C(1.2±0.07)(P0.05);Dosage of ephedrine in group A(5.3±1.2)mg and group B(6.0±1.6)mg were more than group C(0.0±0.0)mg.Systolic pressures at post-anesthetic 5 minute、10 minute in group A[(101.9±2.4)mmHg,(102.3±2.7)mmHg]correspondingly were lower than group C[(112.9±1.8)mmHg,(111.6±2.2)mmHg]respectively(P0.05),and at delivery group A(109.8±2.3)mmHg and group B(113.8±2.0)mmHg were lower than group C(121.4±2.5)mmHg(P0.05).Diastolic pressure at post-anesthetic 10 minutes of group A(59.5±2.0)mmHg was lower than that of group C(68.7±1.5)mmHg(P0.05).But there was no difference at other time among three groups(P0.05).Also there was no difference in terms of heart rate,SpO2,Apgar scores of the neonate,nausea,vomit and chest distress among three groups(P0.05).Hypotension in group A(43.3%)and group B(40%)were more often than group C(P0.05).Conclusion 0.5% bupivacaine 5 mg spinal anesthesia combined with epidural 2% lidocaine 3 mL can provide reliable anesthesia and has the minimal effect on maternal hemodynamics.There was no significant difference between fixed dose group 1.3 mL and height adjusted dose group.
A distributed content-based search engine based on mobile code (2005)

Current search engines crawl the Web, download content, and digest this content locally. For multimedia content, this involves considerable volumes of data. Furthermore, this process covers only publicly available content because content providers are concerned that they otherwise loose control over the distribution of their intellectual property. We present the prototype of our secure and distributed search engine, which dynamically pushes content based feature extraction to image providers. Thereby, the volume of data that is transported over the network is significantly reduced, and the concerns mentioned above are alleviated. The distribution of feature extraction and matching algorithms is done by mobile software agents. We give a description of the search engine's architecture and implementation, quantitative evaluation results, and a discussion of related security mechanism for content protection and server security.
Experimental set-up and results of the process of co-extruded perforated gun propellants (2009)

Enhancement of gun performance can be obtained by increasing the propellant loading density or the energy content of the propellant. Serious consequences of these options are difficulties with regard to ignition and to gun barrel wear. Application of co-layered propellants is a good alternative, since with proper burning properties of the two layers both an increased muzzle velocity and a decreased gas temperature can be obtained. Many investigations focus on co-layered propellant disks, which may only be ignited by ETCignition. The manufacture of co-layered sheet propellant is labour intensive and difficult. Co-extrusion of co-layered propellants is a good alternative and offers a wide range of possible applications. Both non-, single-, and multi-perforated propellants can be produced by relatively simple and well known extrusion processes. These types of co-extruded propellants have superior properties with respect to erosivity and performance. Co-extruded propellants are therefore well suitable for use in direct-fire systems, for the upgrading of munitions of conventional howitzers, and for other systems. Besides, other possibilities are offered like the improvement of the ignition of difficult-to-ignite LOVA propellants, or the release of wear reducing agents shortly after ignition.
Effects of finite register length in digital filtering and the fast Fourier transform (1972)

When digital signal processing operations are implemented on a computer or with special-purpose hardware, errors and constraints due to finite word length are unavoidable. The main categories of finite register length effects are errors due to A/D conversion, errors due to roundoffs in the arithmetic, constraints on signal levels imposed by the need to prevent overflow, and quantization of system coefficients. The effects of finite register length on implementations of linear recursive difference equation digital filters, and the fast Fourier transform (FFT), are discussed in some detail. For these algorithms, the differing quantization effects of fixed point, floating point, and block floating point arithmetic are examined and compared. The paper is intended primarily as a tutorial review of a subject which has received considerable attention over the past few years. The groundwork is set through a discussion of the relationship between the binary representation of numbers and truncation or rounding, and a formulation of a statistical model for arithmetic roundoff. The analyses presented here are intended to illustrate techniques of working with particular models. Results of previous work are discussed and summarized when appropriate. Some examples are presented to indicate how the results developed for simple digital filters and the FFT can be applied to the analysis of more complicated systems which use these algorithms as building blocks.
A role for Ctr9p and Paf1p in the regulation of G1 cyclin expression in yeast (1999)

Entry into the cell cycle in budding yeast involves transcriptional activation of G1cyclin genes and DNA synthesis genes when cells reach a critical size in late G1. Expression of G1cyclins CLN1 and CLN2 is regulated by the transcription factor SBF (composed of Swi4p and Swi6p) and depends on the cyclin-dependent Cdc28 protein kinase and cyclin Cln3p. To identify novel regulators of SBF-dependent gene expression we screened for mutants that fail to activate transcription of G1cyclins. We found mutations in a gene called CTR9. ctr9 mutants are inviable at 37 degrees C and accumulate large cells. CTR9 is identical to CDP1. CTR9 encodes a conserved nuclear protein of 125 kDa containing several TPR repeats implicated in protein-protein interactions. We show that Ctr9p is a component of a high molecular weight protein complex. Using immuno-affinity chromatography we found that Ctr9p associates with polypeptides of 50 and 65 kDa. By mass spectrometry these were identified as Cdc73p and Paf1p. We show that Paf1p, like Ctr9p, is required for efficient CLN2 transcription, whereas Cdc73p is not. Paf1p and Cdc73p were previously reported to be RNA poly-merase II-associated proteins, suggesting that the Ctr9p complex may interact with the general transcription apparatus.
Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of <i>Enterococcus faecalis</i> from the root canal,<i>in vitro</i> (1997)

The effectiveness of 4.0% sodium hypochlorite (NaOCl) used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal was tested in vitro. Root canals contaminated with E. faecalis were treated as follows: (i) irrigation with 2 mL of NaOCl solution and agitation with hand files; (ii) irrigation with 2 mL of NaOCl solution and ultrasonic agitation; (iii) irrigation with NaOCl alternated with hydrogen peroxide. Contaminated canals irrigated with sterile saline solution served as the control. Paper points used to sample bacteria from the root canals were transferred to tubes containing 5 mL of brain heart infusion (BHI) broth. Tubes were incubated and the appearance of broth turbidity was indicative of bacteria remaining in the root canal. There were no statistically significant differences between the experimental groups. However, NaOCl applied by the three methods tested, was significantly more effective than the saline solution (control group) in disinfecting the root canal.
Induction of autoimmune response to the extracellular loop of the HERG channel pore induces QTc prolongation in guinea‐pigs (2016)

We recently demonstrated that anti-SSA/52 kDa Ro antibodies (Abs) from patients with autoimmune diseases and corrected QT (QTc) prolongation directly target and inhibit the human ether-à-go-go-related gene (HERG) K<sup>+</sup> channel at the extracellular pore (E-pore) region, where homology with SSA/52 kDa Ro antigen was demonstrated. We tested the hypothesis that immunization of guinea-pigs with a peptide corresponding to the E-pore region (E-pore peptide) will generate pathogenic inhibitory Abs and cause QTc prolongation. Guinea-pigs were immunized with a 31-amino-acid peptide corresponding to the E-pore region of HERG. On days 10-62 after immunization, ECGs were recorded and blood was sampled for the detection of E-pore peptide Abs. Serum samples from patients with autoimmune diseases were evaluated for reactivity to E-pore peptide by enzyme-linked immunosorbent assay (ELISA), and histology was performed on hearts using Masson's Trichrome. Inhibition of the HERG channel was assessed by electrophysiology and by computational modelling of the human ventricular action potential. The ELISA results revealed the presence of high titres of E-pore peptide Abs and significant QTc prolongation after immunization. High reactivity to E-pore peptide was found using anti-SSA/Ro Ab-positive sera from patients with QTc prolongation. Histological data showed no evidence of fibrosis in immunized hearts. Simulations of simultaneous inhibition of repolarizing currents by anti-SSA/Ro Ab-positive sera showed the predominance of the HERG channel in controlling action potential duration and the QT interval. These results are the first to demonstrate that inhibitory Abs to the HERG E-pore region induce QTc prolongation in immunized guinea-pigs by targeting the HERG channel independently from fibrosis. The reactivity of anti-SSA/Ro Ab-positive sera from patients with connective tissue diseases with the E-pore peptide opens novel pharmacotherapeutic avenues in the diagnosis and management of autoimmune-associated QTc prolongation.