gebhart/patrepeval huggingface dataset:
Protective effects of interferon-γ on squamous-cell carcinoma of head and neck targets in antibody-dependent cellular cytotoxicity mediated by human natural killer cells (1996)

An in vitro model of antibody-dependent cellular cytotoxicity (ADCC) was established, using squamous-cell carcinoma of the head and neck (SCCHN) targets,human/mouse chimeric monoclonal antibodies (cMAbs) SF-25 and 323/A3 and human peripheral blood mononuclear cells (PBMC). We previously showed that natural killer (NK) cells are the main effector population mediating ADCC in the presence of the cMAbs. ADCC was significantly inhibited by the overnight pre-treatment of SCCHN targets with exogenous interferon-gamma (IFN-gamma). This inhibition was dose-dependent, reproducible and consistently observed with various SCCHN cell lines. SCCHN cells pre-treated with IFN-gamma had a significantly higher expression of intercellular adhesion molecule-I (ICAM-I) and major histocompatibility complex (MHC) class I antigens compared with untreated target cells. No differences in expression of the SCCHN-associated antigens on these targets or in the formation of NK-SCCHN conjugates were found, using flow cytometry. IFN-gamma-pre-treated SCCHN cells were less effective in competing with untreated targets in cold target inhibition assays and in inducing cytokine production from NK cells in co-incubation experiments. Protective effects of IFN-gamma on target cell sensitivity to lysis were blocked by pre-treatment of target cells with actinomycin-D or cycloheximide. The susceptibility of the target cells was restored by removal of MHC class I antigens from their surface by acid stripping before ADCC. Our results suggest that the decreased ADCC seen with SCCHN targets pre-treated with IFN-gamma is related to post-binding events, possibly altered signaling from targets to effector cells, and requires protein synthesis in the target cells.
Prediction of cetane number by group additivity and carbon-13 Nuclear Magnetic Resonance (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPrediction of cetane number by group additivity and carbon-13 Nuclear Magnetic ResonanceTimothy H. DeFries, Rodney V. Kastrup, and Doren IndritzCite this: Ind. Eng. Chem. Res. 1987, 26, 2, 188–193Publication Date (Print):February 1, 1987Publication History Published online1 May 2002Published inissue 1 February 1987https://pubs.acs.org/doi/10.1021/ie00062a002https://doi.org/10.1021/ie00062a002research-articleACS PublicationsRequest reuse permissionsArticle Views286Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
High-Frequency Ultrasound Quantitative Analyses of Corneal Scarring Following Excimer Laser Keratectomy (1993)

High-frequency ultrasound signal processing is a noninvasive method that quantitatively grades excimer laser-induced corneal scarring.
A randomized study with or without intensified maintenance chemotherapy in patients with acute promyelocytic leukemia who have become negative for PML-RARα transcript after consolidation therapy: The Japan Adult Leukemia Study Group (JALSG) APL97 study (2007)

To examine the efficacy of intensified maintenance chemotherapy, we conducted a prospective multicenter trial in adult patients with newly diagnosed acute promyelocytic leukemia treated with all-trans retinoic acid and chemotherapy. Of the 302 registered, 283 patients were assessable and 267 (94%) achieved complete remission. Predicted 6-year overall survival in all assessable patients and disease-free survival in patients who achieved complete remission were 83.9% and 68.5%, respectively. A total of 175 patients negative for PML-RARalpha at the end of consolidation were randomly assigned to receive either intensified maintenance chemotherapy (n = 89) or observation (n = 86). Predicted 6-year disease-free survival was 79.8% for the observation group and 63.1% for the chemotherapy group, showing no statistically significant difference between the 2 groups (P = .20). Predicted 6-year survival of patients assigned to the observation was 98.8%, which was significantly higher than 86.2% in those allocated to the intensified maintenance (P = .014). These results indicate that the intensified maintenance chemotherapy did not improve disease-free survival, but rather conferred a significantly poorer chance of survival in acute promyelocytic leukemia patients who have become negative for the PML-RARalpha fusion transcript after 3 courses of intensive consolidation therapy.
Photoferroelectric image storage in antiferroelectric-phase PLZT ceramics (1979)

A recently discovered photoferroelectric (PFE) effect in which the electric-field-induced antiferroelectric (AFE)-to-ferroelectric (FE) phase transition is photoinhibited is used to store nonvolatile gray-scale images in lead lanthanum zirconate titanate (PLZT) ceramics. Stored images exhibit exceptionally high contrast and approximately linear gray-scale reproduction. Images are stored as spatial variations of the volume ratio of FE phase to the initial AFE phase. Localized variations of the ratio of FE to AFE phase produce related variations in light scattering. In the materials studied, the scattering effect partially depolarizes transmitted visible light, and the stored image can be viewed directly or projected onto a screen using crossed linear polarizers.
In vivo native cellular fluorescence and histological characteristics of head and neck cancer. (1998)

Native cellular fluorescence (NCF) represents the innate capacity of tissues to absorb and emit light of a specified wavelength. The ability to define the relationship of in vivo NCF with biological characteristics of neoplastic disease may allow for an improved understanding of the clinical course of disease. Head and neck cancers from 35 patients were evaluated in vivo for NCF characteristics using a xenon lamp-based spectrometer coupled to a handheld fiberoptic probe. Spectral assessment was limited to lambda 450-nm emission characteristics, in which tissues were excited at various wavelengths, ranging from lambda 290 nm to lambda 415 nm, and the intensity of lambda 450 nm emission was recorded. Each cancer was subsequently biopsied and assessed for histological differentiation by a pathologist who was blinded to NCF analysis. Considerable variation in spectral characteristics between head and neck cancers was identified, which was determined, in part, by NCF characteristics of the normal mucosa from the same patient. Poorly differentiated tumors were more likely than well- or moderately differentiated tumors to have lower excitation maxima (P < 0.05 by ANOVA). Most significantly, the tumor differentiation status, as well as the probability of demonstrating recurrent disease, could also be related to the NCF characteristics of the patient's normal mucosa from the same site within the upper aerodigestive tract. NCF analysis may represent an effective tool to identify biological characteristics of head and neck tumors in vivo without the need for invasive biopsies. Results suggest the need to explore the determinants of NCF characteristics expressed by clinically normal mucosa.
Characterization of the Rat Star Gene That Encodes the Predominant 3.5-Kilobase Pair mRNA (1998)

The steroidogenic acute regulatory protein (STAR) participates in steroidogenesis through the mitochondrial transfer of cholesterol to cytochrome P450scc. The rat adrenal Star gene is transcribed as a 3. 5-kilobase pair (kb) and 1.6-kb mRNA with the larger mRNA predominating ( approximately 85% of total) in vivo. Hypophysectomy (HPX) produced a 3-5-fold decrease in Star mRNA along with a loss of adrenal steroids, whereas P450scc mRNA decreased by less than 2-fold. Adrenocorticotropic hormone (ACTH) treatment of HPX rats maximally stimulated steroidogenesis rates within 5 min with over 10-fold elevation of steady state blood levels occurring within 10 min. For intact rats there was a 5-10-fold larger increase, paralleling previously observed elevations of cholesterol-cytochrome P450scc association and metabolism in subsequently isolated adrenal mitochondria. ACTH did not increase either total STAR protein or a group of modified forms until at least 30 min after completion of acute stimulation, indicating that elevated translation of STAR protein cannot alone mediate this acute stimulation. Parallel slow changes in STAR protein and corticosterone formation after ACTH treatment are consistent with participation of STAR forms as co-regulators of these hormonal responses. ACTH stimulation of HPX rats increased Star mRNA by 2.5-fold within 20 min and by 4.5-fold after 1 h, thus preceding the rise in the STAR protein. A 3.5-kb Star cDNA clone isolated from a rat adrenal cDNA library exhibited a 0.9-kb open reading frame and a 2.5-kb 3'-untranslated region (3'-UTR). The open reading frame sequence differed at only 12 amino acids from that of the mouse Star. The rat Star gene seven exons with exon 7 encoding the entire 2.5 kb of 3'-UTR of the 3.5-kb mRNA. The 3'-UTR sequence suggests that 1.6- and 3.5-kb mRNA are formed by an alternative usage of different polyadenylation signals. Multiple UUAUUUA(U/A)(U/A) motifs also suggest additional regulation through this extended 3'-UTR. Although elevation of STAR protein by ACTH does not cause the acute increase in adrenal cholesterol metabolism, changes in the turnover or distribution of an active STAR subfraction cannot be excluded.
Key management for restricted multicast using broadcast encryption (2000)

The problem we address is how to communicate securely with a set of users (the target set) over an insecure broadcast channel. This problem occurs in two application domains: satellite/cable pay TV and the Internet MBone. In these systems, the parameters of major concern are the number of key transmissions and the number of keys held by each receiver. In the Internet domain, previous schemes suggest building a separate key tree for each multicast program, thus incurring a setup cost of at least k log k per program for target sets of size k. In the pay TV domain, a single key structure is used for all programs, but known theoretical bounds show that either very long transmissions are required, or that each receiver needs to keep prohibitively many keys. Our approach is targeted at both domains. Our schemes maintain a single key structure that requires each receiver to keep only a logarithmic number of establishment keys for its entire lifetime. At the same time our schemes admit low numbers of transmissions. In order to achieve these goals, and to break away from the theoretical bounds, we allow a controlled number of users outside the target set to occasionally receive the multicast. This relaxation is appropriate for many scenarios in which the encryption is used to force consumers to pay for a service, rather than to withhold sensitive information. For this purpose, we introduce f-redundant establishment key allocations, which guarantee that the total number of recipients is no more than f times the number of intended recipients. We measure the performance of such schemes by the number of key transmissions they require, by their redundancy f, and by the probability that a user outside the target set (a free-rider) will be able to decrypt the multicast. We prove a new lower bound, present several new establishment key allocations, and evaluate our schemes' performance by extensive simulation.
Pulse-train excitation of sodium for use as a synthetic beacon (1992)

The responses of two-level atoms and of sodium atoms to trains of repetitively pulsed resonance radiation are computed. It is found that the optimum backscatter from mesospheric sodium for a given irradiance is attained when the pulse trains are phase modulated and when the lengths of individual pulses are in the 500–700-ps range, corresponding to a transform-limited bandwidth somewhat smaller than the Doppler-broadened hyperfine-structure pattern of the D2 line.
Hetero-oligomerization-dependent Binding of Pig Oocyte Zona Pellucida Glycoproteins ZPB and ZPC to Boar Sperm Membrane Vesicles (1998)

The zona pellucida surrounding the pig oocyte contains two Mr 55,000 glycoproteins, pZPB and pZPC, which are orthologues of mouse zona proteins ZP1 and ZP3, respectively. We previously reported that isolated boar sperm membrane vesicles possess high affinity binding sites for partially purified pZPB, but not pZPC. Interestingly, co-incubation experiments also implicated pZPB-pZPC complexes as potential ligands. We now report that when depleted of a minor pZPC contaminant by size exclusion chromatography, pZPB lacks independent binding activity. In solid phase binding assays employing immobilized boar sperm membranes, pZPB failed to compete with biotin-(pZPB+pZPC) probe, and biotin-labeled pZPB yielded negligible binding. However, when co-incubated with pZPC prior to the binding assays, pZPB acted as a potent competitor, and biotin-labeled pZPB exhibited high affinity, saturable binding. Binding activity was attributed to pZPB-pZPC heterocomplexes, which were detected in co-incubation mixtures by size exclusion chromatography and Western blot analysis. In the pig, therefore, sperm membranes possess a zona-binding protein with high affinity sites for pZPB-pZPC heterocomplexes, but not free glycoprotein subunits. Consequently, associative interactions between zona molecules can contribute toward both the assembly of the zona matrix and generation of ligands important for sperm-zona interactions.
ANTIBODY-MEDIATED P53 PROTEIN THERAPY PREVENTS LIVER METASTASIS IN VIVO. (2007)

Purpose To test the clinical efficacy of antibody-mediated protein transduction of p53 into cancer cells in vivo in a mouse model of colon cancer metastasis to liver. Methods An Fv fragment of mAb 3E10 was previously shown to penetrate into cancer cells and localize in the nucleus. A His6-tagged Fv-p53 fusion protein was produced in Pichia pastoris X-33 yeast cells transfected with pPICZA-Fv-p53. Fv-p53 was isolated on NiNTA-agarose and assayed for killing CT26.CL25 colon cancer cells in vitro and CT26.CL25 cells injected into the portal vein of syngeneic Balb/c mice. Results Experiments in vitro demonstrated killing of CT26.CL25 cells by Fv-p53 but not Fv or p53 alone, and immunohistochemical staining confirmed that Fv was required for transport of p53 into cells. Prevention of liver metastasis in vivo was tested by portal vein injections of 100 nM Fv-p53 10 minutes and 1 week after injection of cancer cells. Mice were sacrificed 2 weeks after the second injection of Fv-p53 and assigned metastasis scores based on the following criteria: 0 - no liver metastasis; 1 - minimal infiltration (less than 1 cm2 area of metastasis); 2 - mild to moderate infiltration (1-2 cm2 area of metastasis); 3 - severe infiltration (greater than 2 cm2 area of metastasis but the liver was not entirely infiltrated); 4 - complete infiltration. As shown in Table 1, Fv-p53 prevented liver metastasis. Conclusion Fv-p53 treatment had a profound impact on liver metastasis. These results demonstrate the clinical potential of an antibody Fv fragment as a protein transduction domain for intracellular/intranuclear delivery of p53 for prevention of cancer metastasis in vivo.
Alteration of binding properties and cytoskeletal attachment of nerve growth factor receptors in PC12 cells by wheat germ agglutinin. (1982)

Incubation of PC12 cells preloaded with 125I-nerve growth factor (NGF) reveals rapidly and slowly dissociating binding components indicative of a heterogeneous population of receptors. If the cells are previously exposed to wheat germ agglutinin (WGA) for 30 min, NGF now binds to an apparently homogeneous receptor population which exhibit slow dissociation kinetics. Total binding is also reduced by 50%. If WGA is added subsequent to 125I-NGF, total binding is not diminished, but rapidly dissociating receptors occupied with NGF are all converted to the slowly dissociating form. This conversion of receptors occurs rapidly, reaching completion within 2 min at 37 degrees or 4 degrees C, and is unaffected by metabolic energy poisons, suggesting that WGA-induced slowly dissociating receptors are not the product of internalization. The effects of the lectin are blocked by the sugar N-acetyl-D-glucosamine, and the lectin-induced slowly dissociating receptors are converted back to rapidly dissociating receptors by addition of this same sugar. WGA also affects the association of the NGF receptor with the Triton X-100 cytoskeleton. Greater than 90% of bound 125I-NGF becomes associated with Triton X-100 insoluble cytoskeletons in the presence of the lectin, compared with less than 20% before lectin addition. Cytoskeleton association of the NGF receptor by WGA shows similar kinetics as the conversion of rapidly to slowly dissociating receptors. This interaction may be involved in the alteration of NGF-receptor binding properties produced by this lectin.
An Automated Formal Approach to Managing Dynamic Reconfiguration (2006)

Dynamic reconfiguration is the process of making changes to software at run-time. The motivation for this is typically to facilitate adaptive systems which change their behavior in response to changes in their operating environment or to allow systems with a requirement for continuous service to evolve uninterrupted. To enable development of reconfigurable applications, we have developed OpenRec, a framework which comprises a reflective component model plus an open and extensible reconfiguration management infrastructure. Recently we have extended OpenRec to verify whether an intended (re)configuration would result in an application's structural constraints being satisfied. Consequently OpenRec can automatically veto proposed changes that would violate configuration constraints. This functionality has been realized by integrating OpenRec with the ALLOY Analyzer tool via a service-oriented architecture. ALLOY is a formal modelling notation which can be used to specify systems and associated constraints. In this paper, we present an overview of the OpenRec framework. In addition, we describe the application of ALLOY to modelling re-configurable component based systems and highlight some interesting experiences with integrating OpenRec and the ALLOY Analyzer
MetaFIND: A feature analysis tool for metabolomics data (2008)

Standard feature selection techniques may fail to capture the full set of relevant features in the case of high dimensional, multi-collinear metabolomics data. We show that the MetaFIND 'post-feature selection' analysis tool may aid metabolite signature elucidation, feature discovery and inference of metabolic correlations.
[Diagnostic and differential diagnostic value of troponins]. (1996)

In all cases with acute damage of the skeletal muscles (post operationem) the concentrations of TnT and TnI were in normal ranges. - In patients with chronic muscle diseases (M. Duchenne, Dermatomyositis) in 75% the TnT concentrations increased, while TnI concentrations were < 0.4 microgram/l. - In end stage renal disease patients undergoing chronic maintenance hemodialysis we found in 39% constantly elevated concentrations of TnT. TnI in all cases were in normal range. - In acute myocardial infarction in 97% of all patients both Troponins were increased, beginning 2 to 4 hours after onset. A normalization was observed between the 5th and 9th day. Very important is the differentiation between stable, and instable angina pectoris. In stable angina the concentration of both Troponins were in normal range, in contrast in 33 to 41% of all patients with unstable angina TnT and TnI were increased, in 9 to 16% even if the ECG showed no alteration. Elevated TnT or TnI concentrations predicted a significantly increased mortality. After coronary angioplasty and heparin treatment all patients had once more an elevation of TnT and TnI 8 and 27 hours after the invasive therapy. One of these substantial reasons for these minor myocardial cell necrosis is beside the endothelial injury the activation of the platelets and the coagulation system. - During the therapy with hirudin increases of TnT and TnI was not observed. Following cardiac surgery (ACVB) 6 to 48 hours after aortic unclamping 76 patients with TnI < 1 microgram/l had no perioperative myocardial infarction, while 14 patients with TnI concentrations > 1 microgram/l in 71.4% (n = 10) developed an acute infarction. - In patients in critical care units we observed only increased TnI concentrations in shock, especially in septic shock with multiple organ failures.
Liquefaction plant design in the 1990s (1995)

Technological advancements and new design methodologies will continue to increase liquefied natural gas plant efficiency and capacity through the end of the decade. This paper discusses plant life extension, improved gas treating, and recent equipment developments. Among the equipment described are heat exchanger designs, liquid and gas expanders, bearing and seal design, and compressors and drivers. Economics of scale and installation of larger train sizes are briefly discussed. LNG plant reliability and availability are also discussed including scheduled and unscheduled plant downtime, designing for increased train capacity, and LNG plant auxiliary systems optimization.
Sensitive detection of oxidatively modified low density lipoprotein using a monoclonal antibody. (1996)

We have established a new method capable of measuring the very low concentrations of oxidized low density lipoprotein (OxLDL). In our previous study, we obtained a novel murine monoclonal antibody against oxidized lipoproteins (Itabe, H. et al. 1994. J. Biol. Chem. 269: 15274-15279). The epitope of this antibody resides in oxidized products of phosphatidylcholine that can form complexes with polypeptides, including apolipoprotein B. When the monoclonal antibody was precoated onto microtiter wells prior to carrying out a sandwich ELISA using an anti-human apolipoprotein B antibody, it was possible to detect 0.5 ng protein of copper-induced OxLDL. The detection of OxLDL was dependent on the presence of monoclonal antibody and was blocked by oxidized phosphatidylcholine (OxPC). Under the same sandwich ELISA condition, native LDL showed a dose-dependent increase of absorbance that was inhibited by complex of OxPC with BSA. These results suggest the possible occurrence of oxidative modification of human plasma LDL, which is recognized by the antibody against OxPC. The level of LDL oxidation of normal human subjects was found to be 0.52 +/- 0.35 units per 5 mu g protein of LDL, where one unit was defined as the reactivity corresponding to 1 ng of copper-induced OxLDL by this assay. Furthermore, we found that the LDL oxidation level in patients who had been receiving hemodialysis treatment was increased more than eightfold over that of normal subjects. We suggest that LDL in human plasma is oxidatively modified under certain conditions and this method for measurement of OxLDL could be used to study the relationship between in vivo oxidation reaction and various pathological conditions.