gebhart/patrepeval huggingface dataset:
Inactivation of the Escherichia coli priA DNA replication protein induces the SOS response (1991)

Many of the proteins that operate at the replication fork in Escherichia coli have been defined genetically. These include some of the subunits of the DNA polymerase III holoenzyme, the DnaB replication fork helicase, and the DnaG primase. The multiprotein primosome (which includes the DnaB and DnaG proteins), defined biochemically on the basis of its requirement during bacteriophage phi X174 complementary-strand synthesis, could serve as the helicase-primase replication machine on the lagging-strand template. In order to determine if this is the case, we have begun an investigation of the phenotypes of mutants with mutations priA, priB, and priC, which encode the primosomal proteins factor Y (protein n'), n, and n", respectively. Inactivation of priA by insertional mutagenesis resulted in the induction of the SOS response, as evinced by induction of a resident lambda prophage, extreme filamentation, and derepression of an indicator operon in which beta-galactosidase production was controlled by the dinD1 promoter. In addition, the copy numbers of resident pBR322 plasmids were reduced four- to fivefold in these strains, and production of phi X174 phage was delayed considerably. These results are discussed in the context of existing models for SOS induction and possible roles for the PriA protein at the replication fork in vivo.
Distributed knowledge management for autonomous access control in computer networks (2004)

This work discusses a conceptual model for automatic acquisition and processing of knowledge about users and devices in computer networks. It employs autonomous agents for distributed knowledge management and integrates them into an autonomic middleware component. Agents grouped into distributed communities act as mediators between users, devices, and network resources. Communicating between each other they make decisions on whether a certain user or device can be given access to a requested resource. In other words, agents in our system perform user/device authentication, authorisation, and maintenance of user credentials.
Inferring sparsity: Compressed sensing using generalized restricted Boltzmann machines (2016)

In this work, we consider compressed sensing reconstruction from M measurements of K-sparse structured signals which do not possess a writable correlation model. Assuming that a generative statistical model, such as a Boltzmann machine, can be trained in an unsupervised manner on example signals, we demonstrate how this signal model can be used within a Bayesian framework of signal reconstruction. By deriving a message-passing inference for general distribution restricted Boltzmann machines, we are able to integrate these inferred signal models into approximate message passing for compressed sensing reconstruction. Finally, we show for the MNIST dataset that this approach can be very effective, even for M <; K.
Multivariable Servomechanism Controller for Autonomous Operation of a Distributed Generation Unit: Design and Performance Evaluation (2009)

A linear time-invariant (LTI) robust servomechanism controller for islanded (autonomous) operation of a distributed generation (DG) unit and its local load is proposed. The DG unit utilizes a voltage-sourced converter (VSC) as the interface medium. The controller design is obtained by introducing a new optimal controller design procedure, in conjunction with a proposed non-conservative robustness constraint. The proposed controller utilizes 1) an internal oscillator for frequency control and 2) a robust servomechanism controller (RSC) to regulate the island voltage. Despite uncertainty of the load parameters, the proposed controller guarantees robust stability and pre-specified performance criteria, e.g., fast transient response and zero steady-state error. The theoretical aspects of the proposed robust servomechanism controller including the existence conditions, design of the controller, and robust stability analysis of the closed-loop system are studied. Moreover, the performance of the robust servomechanism controller based on 1) simulation studies in the MATLAB software environment, and 2) experiments in a laboratory-scale setup, is presented in this paper. In particular, reference signal tracking and robustness of the closed-loop system with respect to the load parameter uncertainty are investigated. Both computer simulation studies and experimental results confirm that the proposed robust controller provides the specified performance characteristics of the closed-loop system.
High voltage operation (&gt;80 V) of GaN bipolar junction transistors with low leakage (2000)

We have demonstrated the high voltage operation of n-p-n GaN bipolar junction transistors using regrown emitters. Devices were grown by metalorganic chemical vapor deposition on sapphire substrates. The n-type emitter was grown selectively on a base-collector p-n junction diode using a dielectric mask. A thin base (1000 Å) was used to increase the current gain over our previous result with a regrown emitter [J. B. Limb, L. McCarthy, P. Kozodoy, H. Xing, J. Ibbetson, Y. Smorchkova, S. P. DenBaars, and U. K. Mishra, Electron. Lett. 35, 19 (1999)]. The base contacts were better than expected despite the use of a thin base. Common emitter operation showing a voltage operation of over 80 V with negligible leakage has been demonstrated. Room temperature current gain was ∼3 corresponding to a current transfer ratio of ∼0.75. This results in a calculated minority carrier lifetime of about 80 pS in the base.
Antimicrobial activities of secondary metabolites produced by endophytic fungi from Spondias mombin (2000)

We performed a search for bioactive compounds produced by fungal endophytes from Spondias mombin (Anacardiaceae). Culture broth extracts of Guignardia sp., Phomopsis sp. and Pestalotiopsis guepinii were separated by chromatographic methods and tested for biological activities. The crude extracts of these endophytes were tested against fourteen organisms, including actinomycetes, Gram-negative and Gram-positive bacteria, yeast, and filamentous fungi. All fungal extracts inhibited actinomycete growth. Guignardia sp. was also active against Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae, Geotrichum sp., and Penicillium canadensis. Culture extracts ofP. guepinii were active against S. cerevisae, while strains of Phomopsis sp. showed a pronounced antifungal effect against Cladosporium elatum, Mycotypha sp. and S. cerevisae.
Selective oral ROCK2 inhibitor down-regulates IL-21 and IL-17 secretion in human T cells via STAT3-dependent mechanism (2014)

Rho-associated kinase 2 (ROCK2) regulates the secretion of proinflammatory cytokines and the development of autoimmunity in mice. Data from a phase 1 clinical trial demonstrate that oral administration of KD025, a selective ROCK2 inhibitor, to healthy human subjects down-regulates the ability of T cells to secrete IL-21 and IL-17 by 90% and 60%, respectively, but not IFN-γ in response to T-cell receptor stimulation in vitro. Pharmacological inhibition with KD025 or siRNA-mediated inhibition of ROCK2, but not ROCK1, significantly diminished STAT3 phosphorylation and binding to IL-17 and IL-21 promoters and reduced IFN regulatory factor 4 and nuclear hormone RAR-related orphan receptor γt protein levels in T cells derived from healthy subjects or rheumatoid arthritis patients. Simultaneously, treatment with KD025 also promotes the suppressive function of regulatory T cells through up-regulation of STAT5 phosphorylation and positive regulation of forkhead box p3 expression. The administration of KD025 in vivo down-regulates the progression of collagen-induced arthritis in mice via targeting of the Th17-mediated pathway. Thus, ROCK2 signaling appears to be instrumental in regulating the balance between proinflammatory and regulatory T-cell subsets. Targeting of ROCK2 in man may therefore restore disrupted immune homeostasis and have a role in the treatment of autoimmunity.
Hybrid Separation and Detection Device for Analysis of Benzene, Toluene, Ethylbenzene, and Xylenes in Complex Samples (2009)

We present a hybrid system for rapid detection and analysis of benzene, toluene, ethylbenzene, and xylenes (BTEX). The system combines selective and sensitive sensing elements with a fast and miniaturized chromatographic separation method. The sensing elements are an array of microfabricated quartz crystal tuning forks modified with selective molecularly imprinted polymers, and the separation method uses optimized short columns. The high sensitivity and selectivity of the sensing elements together with the help of the separation provides fast detection and analysis of BTEX in real samples containing highly concentrated interfering agents without preconcentration or heating of columns. The low cost, low power consumption, and small size of the hybrid device are particularly suitable for occupational health, industrial safety, and epidemiological applications.
First General, Direct, and Regioselective Synthesis of Substituted Methoxybenzoic Acids by Ortho Metalation (2007)

New general methodology of value in aromatic chemistry based on ortho-metalation sites in o-, m-, and p-anisic acids (1-3) (Scheme 1) is described. The metalation can be selectively directed to either of the ortho positions by varying the base, metalation temperature, and exposure times. Metalation of o-anisic acid (1) with s-BuLi/TMEDA in THF at -78 degrees C occurs exclusively in the position adjacente to the carboxylate. On the other hand, a reversal of regioselectivity is observed with n-BuLi/t-BuOK. With LTMP at 0 degrees C, the two directors of m-anisic acid (2) function in concert to direct introduction of the metal between them while n-BuLi/t-BuOK removes preferentially the proton located ortho to the methoxy and para to the carboxylate (H-4). s-BuLi/TMEDA reacts with p-anisic acid (3) exclusively in the vicinity of the carboxylate. According to these methodologies, routes to very simple methoxybenzoic acids with a variety of functionalities that are not easily accessible by other means have been developed (Table 1).
Paper spray mass spectrometry-based method for analysis of droplets in a gravity-driven microfluidic chip (2013)

This work presents a paper spray mass spectrometry-based method, to analyze microdroplets produced in a gravity-driven microchip. Droplets at ambient pressure were passively transferred from the chip to a paper substrate by the capillary wicking effect. Paper spray ionization was then performed for mass spectrometry (MS) analysis of droplet contents. The qualitative and quantitative analytical performances of this technique for single droplets were demonstrated. This manually controlled interface is straightforward, low-cost and simple to implement. Moreover, paper spray ionization MS holds promise in the direct analysis of real biological/chemical microreaction samples because of its tolerance with complex matrices. As a proof-of-concept example, the droplet-based acetylcholine hydrolysis was carried out to demonstrate the validation of our method for the direct analysis of micro-chemical/biological reactions. We also introduced a flow injection analysis (FIA) system combined with our droplet system to generate a concentration gradient. As a result, the microreaction can be performed at different concentrations and kinetic information can be obtained in one sample injection. In conclusion, the combination of a microdroplet chip with paper spray ionization and the introduction of the FIA system and make our droplet-MS scheme a useful platform for monitoring and analyzing organic-phase chemical/biological reactions.
The retina: oxidative stress and diabetes (2003)

A prominent and early feature of the retinopathy of diabetes mellitus is a diffuse increase in vascular permeability. As the disease develops, the development of frank macular oedema may result in vision loss. That reactive oxygen species production is likely to be elevated in the retina, and that certain regions of the retina are enriched in substrates for lipid peroxidation, may create an environment susceptible to oxidative damage. This may be more so in the diabetic retina, where hyperglycaemia may lead to elevated oxidant production by a number of mechanisms, including the production of oxidants by vascular endothelium and leukocytes. There is substantial evidence from animal and clinical studies for both impaired antioxidant defences and increased oxidative damage in the retinae of diabetic subjects that have been, in the case of animal studies, reversible with antioxidant supplementation. Whether oxidative damage has a causative role in the pathology of diabetic retinopathy, and thus whether antioxidants can prevent or correct any retinal damage, has not been established, nor has the specific nature of any damaging species been characterised.
Differential effects of central and peripheral administration of growth hormone (GH) and insulin-like growth factor on hypothalamic GH- releasing hormone and somatostatin gene expression in GH-deficient dwarf rats (1993)

The roles of GH and insulin-like growth factor-I (IGF-I) in the regulation of hypothalamic GH-releasing hormone (GRH) and somatostatin (SRIH) gene expression were investigated in the GH-deficient dwarf (dw) rat, in which endogenous feedback signals are lacking. Adult male and female dw rats were treated with GH or IGF-I by systemic (sc) administration or intracerebroventricular (icv) infusion, and hypothalamic GRH and SRIH mRNA were determined by Northern blotting and densitometric analysis. Systemic sc injection of rGH (75 micrograms every 12 h for 3 days) decreased GRH mRNA levels in both sexes. However, systemic sc injection of human IGF-I (150 micrograms every 12 h for 3 days) did not affect GRH mRNA levels in either sex despite significant stimulation of body weight gain. The use of a continuous sc infusion, which normalized serum IGF-I levels, and prolongation of the treatment period to 7 days also failed to change GRH mRNA levels. SRIH mRNA was unaffected by systemic administration of either GH or IGF-I. Continuous icv infusion of GH (1 microgram/h for 7 days) decreased GRH mRNA levels in both sexes, but did not alter SRIH mRNA levels. Continuous icv infusion of IGF-I (100 ng/h for 7 days) decreased GRH mRNA in both sexes. In contrast, SRIH mRNA levels were increased in both sexes. IGF-I decreased GRH mRNA levels at icv infusion rates of 100 and 300 ng/h and stimulated SRIH mRNA levels at infusion rates of 30 and 100 ng/h. Food intake was unaffected at these infusion rates. Changes in GRH and SRIH mRNA levels in response to systemic or central GH and IGF-I administration were similar in both sexes, except that the decrease in GRH mRNA levels produced by the icv infusion of IGF-I was greater in female than in male rats. The results provide evidence for a direct inhibitory feedback effect of GH in the central nervous system on the regulation of hypothalamic GRH gene expression that is independent of peripheral IGF-I. IGF-I feedback, in contrast, appears to originate with central nervous system-derived IGF-I and exhibits a stimulatory effect on SRIH mRNA as well as an inhibitory effect on GRH mRNA.
Ultra stable all-fiber telecom-band entangled photon-pair source for turnkey quantum communication applications (2006)

We demonstrate a novel alignment-free all-fiber source for generating telecom-band polarization-entangled photon pairs. Polarization entanglement is created by injecting two relatively delayed, orthogonally polarized pump pulses into a piece of dispersion-shifted fiber, where each one independently engages in four-photon scattering, and then removing any distinguishability between the correlated photon-pairs produced by each pulse at the fiber output. Our scheme uses a Michelson-interferometer configuration with Faraday mirrors to achieve practically desirable features such as ultra-stable performance and turnkey operation. Up to 91.7% two-photon-interference visibility is observed without subtracting the accidental coincidences that arise from background photons while operating the source at room temperature.
The insulin-like growth factor II/mannose 6-phosphate receptor is required for proliferin-induced angiogenesis. (1996)

Proliferin stimulates endothelial cell migration in culture and neovascularization in vivo. Previous studies have demonstrated that proliferin can bind to the insulin-like growth factor II/mannose 6-phosphate receptor, and that binding can be blocked by mannose 6-phosphate. We have now found that this receptor plays an essential role in proliferin-induced angiogenesis. Proliferin binding to endothelial cells is blocked by the addition of mannose 6-phosphate, as is the ability of both recombinant and placental-derived proliferin to stimulate the migration of capillary endothelial cells in vitro and to induce neovascularization in the rat cornea. Consistent with a direct role of this receptor in angiogenesis, insulin-like growth factor II, as well as a mutant form of insulin-like growth factor II that binds to the insulin-like growth factor II/mannose 6-phosphate receptor but not to the insulin-like growth factor I receptor, also stimulate endothelial cell migration and neovascularization.
CUBIT mesh generation environment. Volume 1: Users manual (1994)

The CUBIT mesh generation environment is a two- and three-dimensional finite element mesh generation tool which is being developed to pursue the goal of robust and unattended mesh generation--effectively automating the generation of quadrilateral and hexahedral elements. It is a solid-modeler based preprocessor that meshes volume and surface solid models for finite element analysis. A combination of techniques including paving, mapping, sweeping, and various other algorithms being developed are available for discretizing the geometry into a finite element mesh. CUBIT also features boundary layer meshing specifically designed for fluid flow problems. Boundary conditions can be applied to the mesh through the geometry and appropriate files for analysis generated. CUBIT is specifically designed to reduce the time required to create all-quadrilateral and all-hexahedral meshes. This manual is designed to serve as a reference and guide to creating finite element models in the CUBIT environment.
Top-Gate Staggered a-IGZO TFTs Adopting the Bilayer Gate Insulator for Driving AMOLED (2012)

We report the successful implementation of top-gate staggered amorphous In-Ga-Zn-O (a-IGZO) thin-film transistors (TFTs) with decent performance and environmental stability by adopting the SiO <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</i> /SiN <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</i> bilayer gate-insulator stack. The PECVD SiO <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</i> and SiN <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</i> were used as the first and second gate insulators, respectively, in the TFT to simultaneously ensure the channel/gate-insulator interface properties for device performances and the water impermeability of the gate insulator for effective passivation of the channel layer. It was also found that the cleanliness of the back-channel interface (and thus the effectiveness of the source/drain etching process) is critical for the successful implementation of the top-gate staggered a-IGZO TFTs. In this paper, a two-step wet-etching process for source/drain was used to ensure the quality of the back-channel interface. Finally, we successfully integrated the top-gate staggered a-IGZO TFTs into a working 2.2-in active matrix organic light-emitting display panel, demonstrating the real use of the developed TFTs.
An overview of hard chromium plating using trivalent chromium solutions (2011)

http://www.pfonline.com/articles/an-overview-of-hard-chromium-plating-using-trivalent-chromium-solutions
The design of the control system for ANKA (2002)

ANKA is a low budget light source. It has to be built with minimal cost. This includes a short construction period to keep the personnel cost low. The use of ANKA as an industrial light source on the other hand places tight constraints on the reliability of its components and its operation. In the creation of the control system one has to minimise in-house development and maximise the use of commercial-quality products. Here, the term commercial-quality applies equally to off-the-shelf-products and to products in the public domain or from other accelerator centres that are field proven and are documented at a level equal to commercial products. It has been our goal to search for the simplest model and thus the most reliable one. Our extensive investigations have shown that none of the actions and responses of the control system for ANKA need a real-time operating system. Therefore, we have disposed of the whole middle layer of the control system including VME crates in favour of a fieldbus network which connects the individual devices directly to the console computers. The consoles will be PCs running Windows NT. The fieldbus candidate is LonWorks from Echelon which provides plug-and-play and requires almost no additional development. On the software side we have opted for TACO, an object oriented control system environment developed at the ESRF.
Rapeseed meal‐glucosinolates and their antinutritional effects Part 5. Animal reproduction (1994)

Although there is no clear evidence to fully describe the mechanism involved in glucosinolate-related effects on animal reproduction, lowered fertility in animals fed diets with rapeseed meal (RSM) inclusion is related to glucosinolate content in the diet. Negative effects can result both from multidirectional action of glucosinolates, malnutrition of mother due to the thyroid dysfunction, transfer of goitrogenic compounds to foetus and reduced transfer of nutritive compounds (e.g. iodine) through the placenta. The degree of reproduction impairment depends both on glucosinolate content and on the type of animal. Pregnant female rats are sensitive to the presence of glucosinolates, and the first symptoms of fertility impairment and lowering of offspring survival rate may occur at low glucosinolate levels, e.g. with diets containing low glucosinolate rapeseed meal (LG-RSM). In the case of swine, the limiting value above which sows fertility may be impaired is 4 mumol of total glucosinolates per g diet and 8 mmol of daily intake of these compounds. Opinions on the permitted RSM inclusion in diet for hens are very divergent. Some authors recommend limiting of the RSM inclusion to 10%, while others did not find any lowering of egg production in hens fed with two- or three-fold greater RSM inclusion rates. Most long-term experiments with heifers, cows and ewes indicate that ruminants tolerate the level of glucosinolate in LG-RSM although feeding this fodder as the only high-protein concentrate component may lead to impaired fertility.
A 2450 Mhz Slab-Loaded Direct Contact Applicator with Choke (1980)

A Teflon-slab-loaded, direct contact microwave diathermy applicator has been developed. It produces minimal leakage radiation during effective heating of simulated planar tissue models. Because of TEM mode excitation, heating patterns are relatively uniform.
Phosphoinositide 3-Kinase Signalling Pathways in Tumor Progression, Invasion and Angiogenesis (2004)

A more complete understanding of the role of the PI3 kinase pathway in cancer will lead the way to the development of more potent and selective inhibitors which should be a useful adjunct to conventional therapies, potentially interfering with tumor progression at several pivotal points; in particular cell survival, invasion and angiogenesis.