gebhart/patrepeval huggingface dataset:
Superfast multifrequency phase-shifting technique with optimal pulse width modulation (2011)

The technique of generating sinusoidal fringe patterns by defocusing squared binary structured ones has numerous merits for high-speed three-dimensional (3D) shape measurement. However, it is challenging for this method to realize a multifrequency phase-shifting (MFPS) algorithm because it is difficult to simultaneously generate high-quality sinusoidal fringe patterns with different periods. This paper proposes to realize an MFPS algorithm utilizing an optimal pulse width modulation (OPWM) technique that can selectively eliminate high-order harmonics of squared binary patterns. We successfully develop a 556 Hz system utilizing a three-frequency algorithm for simultaneously measuring multiple objects.
Achieving energy efficient and secure communication in wireless sensor networks (2006)

Wireless sensor networks (WSN) are increasingly become viable solutions to many challenging problems and will successively be deployed in many areas in the future such as in environmental monitoring, business, and military applications. The huge challenge in WSN is due to inherent resource and computing constraints. Because the sensor nodes are battery powered, increasing the autonomous lifetime of a WSN is a challenging optimization problem. Transmission of data is one of the most energy expensive tasks a node undertakes - using data compression to reduce the number of bits sent reduces energy expended for transmission. Data compression which highly reduces the communication overhead by aggregating and compressing data packets is performed at intermediate sensor nodes. However, deploying new technology, without security in mind has often proved to be unreasonably dangerous. The problem of securing these networks emerges more and more as a hot topic. Symmetric key cryptography is commonly seen as infeasible on such networks. Public key cryptography has its own key distribution problem. In contrast to this prejudice this paper presents a method to increase the lifetime of a WSN by minimizing the energy cost of transporting information from a set of sources nodes to the sink nodes and for achieving security we have used a new public-key encryption technology called identity-based encryption (IBE) which allows to calculate a public key directly from a user's identity. By calculating public keys instead of generating them randomly, many of the difficulties that make encryption technology difficult to deploy and maintain are eliminated, making encrypted communications much easier to implement than in the past.
Interleukin-6 in the Injured Patient (1996)

Interleukin-6 appears to play an active role in the postinjury immune response, making it an attractive therapeutic target in attempts to control hyperinflammatory provoked organ injury.
In-hospital and thirty-day outcomes of the SAPIEN 3 Ultra balloon-expandable transcatheter aortic valve: the S3U registry (2020)

This first multicentre international experience of transfemoral TAVI with the SAPIEN 3 Ultra transcatheter heart valve shows good in-hospital and 30-day clinical outcomes.
Antitoxin Characteristic of Licorice Extract: The Inhibitory Effect on Aflatoxin Production in <scp><i>A</i></scp><i>spergillus parasiticus</i> (2014)

Abstract Recent investigations revealed the effects of herbal extracts on both fungal growth as well as aflatoxins production. In the present study, we tried to evaluate antifungal activity as well as antitoxin activity of G lycyrrhiza glabra (licorice) extract. Strain American Type Culture Collection 15517 of A spergillus parasiticus was used to perform antifungal susceptibility test according to C linical and L aboratory S tandards I nstitute document M 27‐ A 3, and the rate of aflatoxin production was determined using high‐performance liquid chromatography technique after exposure to different concentrations of licorice extract. Quantitative changes in the expression of the aflR gene were analyzed by measuring the cognate aflR mRNA level with quantitative real‐time reverse‐transcription polymerase chain reaction assay. Our obtained results demonstrated the inhibitory effect of licorice extract on A spergillus parasiticus growth at 500 mg/mL of licorice extract. In addition, a significant decrease in aflatoxin production was revealed at the same concentration. However, the production of aflatoxin B 1 was entirely inhibited in 10 g/mL of licorice extract. The level of aflR gene expression was significantly decreased after the exposure of fungal cells to 500 g/mL of licorice extract. Evaluation of the antifungal and antitoxin activity of licorice extract on A spergillus parasiticus revealed its antifungal properties as well as its effective ability to decrease aflatoxin production. Practical Application Several classes of synthetic antifungal drugs are used today to treat fungal infections in humans and crops. However, this antifungal therapy is not always effective because of emerging of drug‐resistant strains. Therefore, using effective natural antifungal agents with fewer side effects is a promising approach to combat fungal diseases either in medicine or in agriculture. In the present study, we introduced the licorice extract as a novel natural compound to inhibit the growth and production of aflatoxin in the filamentous fungi A spergillus parasiticus and evaluated this natural herbal extract as an antifungal agent.
Occurrence of Indole-3-Acetic Acid-Producing Bacteria on Pear Trees and Their Association with Fruit Russet (1998)

ABSTRACT A relatively high percentage of epiphytic bacteria on pear leaf and fruit surfaces had the ability to produce indole-3-acetic acid (IAA) in culture media supplemented with tryptophan. While over 50% of the strains produced at least small amounts of IAA in culture, about 25% of the strains exhibited high IAA production as evidenced by both colorimetric and high-performance liquid chromatography analysis of culture supernatants. A majority of the strains that produced high amounts of IAA were identified as Erwinia herbicola (Pantoea agglomerans), while some strains of Pseudomonas syringae, Pseudomonas viridiflava, Pseudomonas fluorescens, Pseudomonas putida, and Rahnella aquaticus that produced high amounts of IAA also were found on pear. Fruit russeting was significantly increased in 39 out of 46 trials over an 8-year period in which IAA-producing bacteria were applied to trees compared with control trees. A linear relationship was observed between fruit russet severity and the logarithm of the population size of different IAA-producing bacteria on trees in the 30 days after inoculation, when normalized for the amount of IAA produced by each strain in culture. On average, the severity of fruit russet was only about 77% that on control trees when trees were treated at the time of bloom with Pseudomonas fluorescens strain A506, which does not produce IAA. Both total bacterial populations on pear in the 30-day period following full bloom and fruit russet severity varied greatly from year to year and in different commercial orchards over a 10-year period. There was a strong linear correlation between the logarithm of total bacterial population sizes and fruit russet severity.
Precision chemical heating for diagnostic devices (2015)

Decoupling nucleic acid amplification assays from infrastructure requirements such as grid electricity is critical for providing effective diagnosis and treatment at the point of care in low-resource settings. Here, we outline a complete strategy for the design of electricity-free precision heaters compatible with medical diagnostic applications requiring isothermal conditions, including nucleic acid amplification and lysis. Low-cost, highly energy dense components with better end-of-life disposal options than conventional batteries are proposed as an alternative to conventional heating methods to satisfy the unique needs of point of care use.
Visible Light inter-vehicle Communication for platooning of autonomous vehicles (2016)

In this paper, we study a use of Visible Light Communication (VLC) technology for a platoon of autonomous vehicles. We present a low-cost, low-latency and simple outdoor VLC prototype, which can be installed as a vehicular tail-lighting system. The architecture of our VLC system is introduced, followed by performance evaluation with an especial attention on the VLC link resilience to ambient noise and communication range. Through the experiments, we observe that a use of proper optical filter stage at the receiver side, together with narrowing the transmitter Field-of-view (FOV), result in an extended communication range and make the VLC system more resilient to the ambient noises. Experimental results show that the system can provide 30 meters of inter-vehicle communication with 36 ms of latency, yet on sunny day conditions. The benefit of using the VLC system for platooning control is showed using a Simulink system that integrates our VLC platform for inter-communications to simulates the performance of autonomous vehicles platoon.
Effect of antibody orientation on immunosorbent performance (1996)

The impact of antibody orientation on immunosorbent efficiency was quantitatively assessed. A pH-dependent murine monoclonal antibody (Mab) against human protein C (hPC), recombinant hPC (rhPC) and two different immobilization chemistries and matrices were used as model systems. The lysyl groups of the rhPC were covalently modified with an acetic acid ester of N-hydroxysuccinimide and this modified rhPC was used as a Fab masking agent (FMA). The FMA was used to mask the antigen binding regions (Fab) of the Mab prior to and during covalent immobilization. Thereafter, the residual active sites of the support were inactivated and the FMA was removed. Mab was immobilizeed at low bead-averaged densities of about 0.4-1.1 mg Mab/mL matrix to minimize local density effects. Immunosorbents made using masked Mab (oriented coupling) gave antigen binding efficiencies (nAg) of 42-48% compared with 18-22% for those made by random coupling. The amount of (Fab)2 released from pepsin digestion of immunosorbents was about 3-4-fold higher for matrices having been made with FMA-masked Mab relative to unmasked Mab. Thus, the (Fab)2 accessibility to pepsin correlates well with higher functional efficiency (nAg) and serves as a measure of orientation. In summary, at low Mab density and a 2:1 molar rhPC to Mab binding stoichiometry, about 80% or more of the Mab randomly coupled through amino moieties was improperly oriented relative to oriented coupled Mab, which correlated with about 50% of lost Mab functionality upon immobilization.
Metastasis of unknown origin: The role of fine‐needle aspiration cytology (1998)

Metastasis of unknown origin (MUO) manifests either as a tumor deposit in an organ, often cervical lymphadenopathy, or as a multiorgan carcinomatosis. As a diagnostic and therapeutic challenge, the definition of its cell type and possibly its origin is imperative, although a limited diagnostic investigation is usually recommended. At Hines VA Hospital, from 1986 to 1994, there were 116 cases with admitting clinical diagnosis of MUO, evaluated by fine-needle aspiration cytology (FNAC). The ages of patients ranged from 29 to 82 years (mean 56 years). All were male; Caucasians predominated. The FNAC examination with Diff-Quik and Papanicolaou smears were complemented with special and immunostains in 48 cases (41%) and electron microscopy in 93 cases (80%). The FNAC involved lymph nodes in 57 cases (49%), liver in 27 cases (23%), skin in 22 cases (19%), bone in six cases (5%), lung in three cases (3%), and pleura in one case (1%). The neoplasm was generally cell typed, and there were 60 adenocarcinomas/poorly differentiated carcinomas, 26 small-cell carcinomas (SCC), 23 squamous-cell carcinomas (SQCC), and six undifferentiated large-cell carcinomas. The primary source was defined in 30 cases (26%), consisting of lung in 11 cases, prostate in nine, kidney in four, colon in four, pleura in one, and peritoneum in one. Clinical correlation also supported a lung origin for the SCC, while the majority of the SQCC in upper and middle cervical lymph nodes were considered head-and-neck tumors. Our results affirm that FNAC is a viable and simple procedure in MUO investigations. Diagn. Cytopathol. 1998;18:319–322. © 1998 Wiley-Liss, Inc.
Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths (2006)

The involvement of telomerase in cellular immortalization and senescence has often been assessed by means of telomerase expression at the RNA level and quantification of telomerase activity by the telomeric repeat amplification protocol assay. However, these methods either neglected the existence of various telomerase splice variants, or ignored the nonconventional functions of telomerase independent of its ability to elongate and maintain telomere length. Immunodetection of telomerase is now being recognized as a necessary approach to precisely elucidate its roles in oncogenesis and senescence. A few antibodies directed against the catalytic subunit of the human telomerase (hTERT) are currently used but their specificity is not always demonstrated. A survey of the literature showed inconsistencies and led us to comparatively re-evaluate the most frequently used antibodies. Surprisingly, mass spectrometry, two-dimensional gel analysis and immunofluorescent experiments revealed that the most frequently used hTERT immunoprobe, a mouse monoclonal antibody that was claimed to be directed against an hTERT protein epitope, in fact recognizes nucleolin rather than telomerase. Our findings have interesting implications regarding the biology of nucleolin and telomerase in the context of pathophysiological investigations recently carried out.
The co‐chaperone p23 promotes prostate cancer motility and metastasis (2014)

Prostate cancer is an androgen receptor (AR)-dependent malignancy at initiation and progression, therefore hormone therapy is the primary line of systemic treatment. Despite initial disease regression, tumours inevitably recur and progress to an advanced castration-resistant state a major feature of which is metastasis to the bone. Up-regulation of AR cofactors and chaperones that overcome low hormone conditions to maintain basal AR activity has been postulated as a mechanism of therapy relapse. p23, an essential component of the apo-AR complex, acts also after ligand binding to increase AR transcriptional activity and target gene expression, partly by increasing chromatin-loaded holo-receptor-complexes. Immunohistochemical studies have demonstrated increased p23 expression in advanced prostate cancer. Here, we further characterise p23 roles in AR signalling and show that it modulates cytosolic AR levels in the absence of hormone, confirming a chaperoning function in the aporeceptor complex and suggesting p23 upregulates AR signalling at multiple stages. Moreover, p23 protein levels significantly increased upon treatment with not only androgen but also clinically relevant anti-androgens. This was in contrast to the HSP90 inhibitor 17-AAG, which did not modulate expression of the cochaperone - important given the HSP90-independent roles we and others have previously described for p23. Further, we demonstrate p23 is implicated in prostate cancer cell motility and in acquisition of invasiveness capacity through the expression of specific genes known to participate in cancer progression. This may drive metastatic processes in vivo since analysis of prostate tumour biopsies revealed that high nuclear p23 significantly correlated with shorter survival times and with development of metastases in patients with lower grade tumours. We propose that increased p23 expression may allow cells to acquire a more aggressive phenotype, contributing to disease progression, and that p23 is a plausible secondary target in combination with HSP90 inhibition as a potential therapy for advanced prostate cancer.
Functional synapses are formed between human NTera2 (NT2N, hNT) neurons grown on astrocytes (1999)

The formation of functional synapses is a late milestone of neuronal differentiation. The establishment of functional synapses can be used to assess neuronal characteristics of different cell lines. In the present study, we examined the in vitro conditions that influence the ability of human neurons derived from the NT2 cell line (NT2N neurons) to establish synapses. The morphologic, immunologic, and electrophysiologic characteristics of these synapses was examined. In the absence of astrocytes, NT2N neurons rarely formed synapses and their action potentials were weak and uncommon. In contrast, when plated on primary astrocytes, NT2N neurons were able to form both glutamatergic excitatory (71%) and GABAergic inhibitory (29%) functional synapses whose properties (kinetics, ion selectivity, pharmacology, and ultrastructure) were similar to those of synapses of neurons in primary cultures. In addition, coculture of NT2N neurons with astrocytes modified the morphology of the neurons and extended their in vitro viability to more than 1 year. Because astrocyte-conditioned medium did not produce these effects, we infer that direct contact between NT2N neurons and astrocytes is required. These results suggest that NT2N neurons are similar to primary neurons in their synaptogenesis and their requirement for glial support for optimal survival and maturation. This system provides a model for further investigations into the neurobiology of synapses formed by human neurons.
The Effect of Ln(III)/An(III) Separation on Feasibility of TRU Recycling in PWRs (2006)

This work examines the effect of recycling Ln(III) fission products together with An(III) TRU in existing PWRs without separating them as commonly assumed. TRU recycling in three different fuel forms was studied: U-MOX, Th-MOX, and Fertile Free Fuel. We analyzed the effect of Ln(III) separation on fuel cycle length, efficiency of TRU destruction, and the core reactivity feedback coefficients. Two-dimensional transport and fuel assembly burnup code BOXER was used for the analysis. The full core characteristics were approximated through the use of Linear Reactivity Model.\nThe results show that separation of Ln(III) from An(III) is not mandatory but results in significant deterioration of the PWR core characteristics: Larger TRU loading, reduced control materials reactivity worth, reduced TRU burning efficiency, and less favourable reactivity feedback coefficients. However, the decision on whether to recycle or to separate Ln fission products should be based on comprehensive comparison of the costs associated with each option.
DNA-binding activity of papillomavirus proteins (1987)

We demonstrate DNA binding by papillomavirus (PV) open reading frame (ORF) proteins that correspond to the early transforming and trans-activating (E6 and E2) and late structural regions (L2 and L1) from bovine PV type 1 and human PV types 6b and 16. All PV proteins were synthesized in Escherichia coli and had a common 13-amino-acid leader sequence from the expression vector pRA10. Antibodies have been generated in rabbits against these PV proteins. The PV ORF proteins bind double-stranded DNA, and this activity is demonstrated to be inherent to the PV proteins. DNA-binding activity by PV proteins is optimal at 50 mM NaCl and at pH 7.0. For some PV proteins (e.g., bovine PV type 1 E2), DNA binding is enhanced at a lower pH (pH 6.0) and NaCl concentration (50 to 100 mM). DNA binding is inhibited by the appropriate antibodies. The possible significance of these findings is discussed in relation to the genetic and structural evidence on the function of these ORFs.
Structure and Mechanism of an Amino Acid Antiporter (2009)

Virulent enteric pathogens such as Escherichia coli strain O157:H7 rely on acid-resistance (AR) systems to survive the acidic environment in the stomach. A major component of AR is an arginine-dependent arginine:agmatine antiporter that expels intracellular protons. Here, we report the crystal structure of AdiC, the arginine:agmatine antiporter from E. coli O157:H7 and a member of the amino acid/polyamine/organocation (APC) superfamily of transporters at 3.6 A resolution. The overall fold is similar to that of several Na+-coupled symporters. AdiC contains 12 transmembrane segments, forms a homodimer, and exists in an outward-facing, open conformation in the crystals. A conserved, acidic pocket opens to the periplasm. Structural and biochemical analysis reveals the essential ligand-binding residues, defines the transport route, and suggests a conserved mechanism for the antiporter activity.
BioPen: direct writing of functional materials at the point of care (2014)

Rapid and precise patterning of functional biomaterials is desirable for point-of-care (POC) tissue engineering and diagnostics. However, existing technologies such as dip-pen nanolithography and inkjet printing are currently unsuitable for POC applications due to issues of cost and portability. Here, we report the development of 'BioPen', a portable tool for continuous, defined and scalable deposition of functional materials with micrometer spatial resolution and nanolitre volumetric resolution. BioPen is based upon the ballpoint pen but with multiple "ink sources" (functional material solutions) and with an apparatus that can be optimized for writing living cells, proteins, nucleic acids, etc. We demonstrate POC detection of human immunodeficiency virus type 1 (HIV-1) nucleic acid by writing on paper with BioPen using "ink" consisting of nucleic acid probes and nucleic acid-modified gold nanoparticles. We also demonstrate POC tissue engineering by writing a continuous pattern of living, functional, interconnected cells with a defined extracellular environment. Because it is simple, accurate, inexpensive and portable, BioPen has broad potential for POC detection of diagnostic biomarkers, and for POC engineering of tissues for a range of healing applications.
Antigenic Relationships of 14 Treponemes Demonstrated by Immunofluorescence (1967)

Immunofluorescence was used as an aid in the antigenic grouping of 14 cultivable treponemes. Antisera were prepared versus each treponemal strain, and the antiglobulins were conjugated with fluorescein isothiocyanate. A common antigen-antibody system, detected in the strains studied, was removed by absorption of each conjugate with Reiter or Borrelia vincentii treponemes. Thus, five categories based on shared group-specific antigens were revealed. Serogroup I: Reiter, English Reiter, Kazan, Kazan numbers 2, 4, 5, and 8. Serogroup II: Nichols and Noguchi. Serogroup III: three oral treponemes, MRB, FM, and N-39. Serogroup IV: B. vincentii. Serogroup V: Treponema zuelzerae. The five serogroups apparently are related by an immunofluorescent common antigen.
A review of graphite and gold surface studies for use as substrates in biological scanning tunneling microscopy studies (1992)

The current status of biological Scanning Tunneling Microscopy (STM) investigations and the importance of using a well-characterized substrate are discussed. The findings of over two years of experiments and over 1,000 images obtained on gold substrates prepared by a variety of different methods are statistically summarized and compared to a very flat reference substrate, highly oriented pyrolytic graphite (HOPG). In an effort to begin to corroborate STM results with those obtained from other more established techniques, the results of Auger Electron Spectroscopy (AES) and Electron Spectroscopy for Chemical Analysis (ESCA) of biomolecular STM samples are presented.